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<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PIN7</accession>
    <name>A0A2D0PIN7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Protein kinase C-binding protein 1 isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">zmynd8</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="20">SDA103</strain>
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    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
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            <location evidence="2">Chromosome</location>
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            <location evidence="1">Nucleus</location>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:histone H3 reader activity"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:transcription corepressor activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Bromo_RACK7"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd15538">
        <property type="entry name" value="PHD_PRKCBP1"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd20160">
        <property type="entry name" value="PWWP_PRKCBP1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Protein kinase C-binding protein 1 isoform C"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Bromodomain-like"/>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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        <property type="entry name" value="MYND_ZMYND11_ZMYD8"/>
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        <property type="entry name" value="PRKCBP1_PHD"/>
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        <property type="entry name" value="PWWP_dom"/>
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        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
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        <property type="entry name" value="ZMYND8"/>
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        <property type="entry name" value="ZMYND8_Bromo_dom"/>
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        <property type="entry name" value="ZMYND8_CC"/>
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        <property type="entry name" value="Znf_FYVE_PHD"/>
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        <property type="entry name" value="Znf_MYND"/>
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    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
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    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
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    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46453">
        <property type="entry name" value="PROTEIN KINASE C-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN KINASE C-BINDING PROTEIN 1 ISOFORM X1"/>
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        <property type="entry name" value="DUF3544"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="FYVE/PHD zinc finger"/>
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        <property type="entry name" value="HIT/MYND zinc finger-like"/>
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        <property type="entry name" value="Tudor/PWWP/MBT"/>
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        <property type="entry name" value="BROMODOMAIN_2"/>
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        <property type="entry name" value="PWWP"/>
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        <property type="entry name" value="ZF_MYND_1"/>
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        <property type="entry name" value="ZF_PHD_1"/>
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    <keyword evidence="9 12" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="21" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="21" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13" id="KW-0863">Zinc-finger</keyword>
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    <sequence length="1174" mass="128444" checksum="6799577AF8217936" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PN20</accession>
    <name>A0A2D0PN20_ICTPU</name>
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        <name evidence="3" type="primary">osm</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017306816.1">
        <property type="nucleotide sequence ID" value="XM_017451327.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PN20"/>
    <dbReference type="GeneID" id="108255387"/>
    <dbReference type="KEGG" id="ipu:108255387"/>
    <dbReference type="CTD" id="5008"/>
    <dbReference type="OrthoDB" id="8922642at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012045035" evidence="1">
        <location>
            <begin position="24"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017306816.1"/>
        </source>
    </evidence>
    <sequence length="175" mass="20098" checksum="BE1E3A0F6E6B4CC4" modified="2017-12-20" version="1">MKSTAGIQTLCFVLSVNLCTLTAFPADLCRMEAILKRVKHELQKLQSGLDIHVKHNGEFIHHLMISKSLPTPQQYKSAACGLSYMEKALEEIEKHQKLIVNSEQIENLFKDVINRLKTHLPVYAHHTLGKCDNPPQLSWPESIFKAKQWTWNFLDSSDKFLQDLIHLLECHEANP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PNJ3</accession>
    <name>A0A2D0PNJ3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Methylcytosine dioxygenase TET</fullName>
            <ecNumber evidence="14">1.14.11.80</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18 19 20" type="primary">tet3</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18 19 20" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18 19 20">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming during embryonic development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>a 5-formyl-2'-deoxycytidine in DNA + 2-oxoglutarate + O2 = a 5-carboxyl-2'-deoxycytidine in DNA + succinate + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53832"/>
            <dbReference type="Rhea" id="RHEA-COMP:13656"/>
            <dbReference type="Rhea" id="RHEA-COMP:13657"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:137731"/>
            <dbReference type="ChEBI" id="CHEBI:137732"/>
            <dbReference type="EC" id="1.14.11.80"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a 5-hydroxymethyl-2'-deoxycytidine in DNA + 2-oxoglutarate + O2 = a 5-formyl-2'-deoxycytidine in DNA + succinate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:53828"/>
            <dbReference type="Rhea" id="RHEA-COMP:13315"/>
            <dbReference type="Rhea" id="RHEA-COMP:13656"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:136731"/>
            <dbReference type="ChEBI" id="CHEBI:137731"/>
            <dbReference type="EC" id="1.14.11.80"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 5-methyl-2'-deoxycytidine in DNA + 2-oxoglutarate + O2 = a 5-hydroxymethyl-2'-deoxycytidine in DNA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:52636"/>
            <dbReference type="Rhea" id="RHEA-COMP:11370"/>
            <dbReference type="Rhea" id="RHEA-COMP:13315"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:85454"/>
            <dbReference type="ChEBI" id="CHEBI:136731"/>
            <dbReference type="EC" id="1.14.11.80"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="14">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">The zinc ions have a structural role.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the TET family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.80" evidence="14"/>
    <dbReference type="RefSeq" id="XP_017306991.1">
        <property type="nucleotide sequence ID" value="XM_017451502.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017306992.1">
        <property type="nucleotide sequence ID" value="XM_017451503.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053530522.1">
        <property type="nucleotide sequence ID" value="XM_053674547.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108255510"/>
    <dbReference type="KEGG" id="ipu:108255510"/>
    <dbReference type="CTD" id="200424"/>
    <dbReference type="GeneTree" id="ENSGT00940000157631"/>
    <dbReference type="OrthoDB" id="8854879at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070579">
        <property type="term" value="F:DNA 5-methylcytosine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141166">
        <property type="term" value="P:chromosomal 5-methylcytosine DNA demethylation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024779">
        <property type="entry name" value="2OGFeDO_JBP1/TET_oxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040175">
        <property type="entry name" value="TET1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046942">
        <property type="entry name" value="TET_oxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23358">
        <property type="entry name" value="METHYLCYTOSINE DIOXYGENASE TET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23358:SF4">
        <property type="entry name" value="METHYLCYTOSINE DIOXYGENASE TET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12851">
        <property type="entry name" value="Tet_JBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01333">
        <property type="entry name" value="Tet_JBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 14 18" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 14" id="KW-0408">Iron</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Methylcytosine dioxygenase TET1-3 oxygenase" evidence="16">
        <location>
            <begin position="1102"/>
            <end position="1775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="50"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="206"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="283"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="547"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="657"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="750"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="892"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1270"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1465"/>
            <end position="1642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1665"/>
            <end position="1689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="207"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="223"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="240"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="294"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="318"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="333"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="369"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="388"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="657"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="713"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="772"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="803"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1279"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1300"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="1473"/>
            <end position="1483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1574"/>
            <end position="1583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1669"/>
            <end position="1685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017306991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017306992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_053530522.1"/>
        </source>
    </evidence>
    <sequence length="1874" mass="204770" checksum="EA06A71D64100A12" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PUI1</accession>
    <name>A0A2D0PUI1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">znrf2a</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="RefSeq" id="XP_017309689.1">
        <property type="nucleotide sequence ID" value="XM_017454200.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PUI1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005168"/>
    <dbReference type="GeneID" id="108256919"/>
    <dbReference type="KEGG" id="ipu:108256919"/>
    <dbReference type="CTD" id="100124611"/>
    <dbReference type="OMA" id="PLCSKFM"/>
    <dbReference type="OrthoDB" id="10057496at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16695">
        <property type="entry name" value="mRING-CH-C4HC2H_ZNRF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000363">
        <property type="entry name" value="E3 ubiquitin-protein ligase ZNRF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051878">
        <property type="entry name" value="ZNRF_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46661">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ZNRF1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46661:SF3">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ZNRF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="15" id="KW-0458">Lysosome</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="19">
        <location>
            <begin position="154"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017309689.1"/>
        </source>
    </evidence>
    <sequence length="197" mass="20965" checksum="3DAABDA2E222920C" modified="2017-12-20" version="1">MGPKQSSPAGSARLRAYSGSDVPTSTTTAAGSGSASAAAIRYYTGNQQQVGARGRYAADGTRPNSLFQSTFIAQGSETQSDDDADGDRSQSGPRLLIGSLPAHLSPHLLGAGFNCPVCSKFVASDEIDLHLVMCLTKPRLSYNEDVLMKDSGECAICLDDMVQGDTIARLPCLCIYHKGCIDEWFEVNRSCPEHPTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PXV7</accession>
    <name>A0A2D0PXV7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="16">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21 22" type="primary">yrk</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21 22" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21 22">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 16">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="16"/>
    <dbReference type="RefSeq" id="XP_017309950.1">
        <property type="nucleotide sequence ID" value="XM_017454461.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017309951.1">
        <property type="nucleotide sequence ID" value="XM_017454462.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PXV7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030003"/>
    <dbReference type="GeneID" id="108257057"/>
    <dbReference type="KEGG" id="ipu:108257057"/>
    <dbReference type="CTD" id="337571"/>
    <dbReference type="OMA" id="YRMACAP"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14203">
        <property type="entry name" value="PTKc_Src_Fyn_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000553">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000022">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000016">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000120">
        <property type="entry name" value="Tyrosine-protein kinase Fyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
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        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
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        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 16 21" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0564">Palmitate</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 14" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="3 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 16" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="SH3" evidence="18">
        <location>
            <begin position="86"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="17">
        <location>
            <begin position="153"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="275"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50001"/>
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    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
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    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017309950.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017309951.1"/>
        </source>
    </evidence>
    <sequence length="541" mass="60693" checksum="A1B68ABB032DFB91" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PZ04</accession>
    <name>A0A2D0PZ04_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanine nucleotide-binding protein subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">gngt1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">G proteins are composed of 3 units; alpha, beta and gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Lipid-anchor</topology>
            <orientation evidence="1 9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the G protein gamma family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017310671.1">
        <property type="nucleotide sequence ID" value="XM_017455182.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PZ04"/>
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    <dbReference type="OMA" id="CEEVMEY"/>
    <dbReference type="OrthoDB" id="9933679at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00068">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.260.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001770">
        <property type="entry name" value="G-protein_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13809">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00321">
        <property type="entry name" value="GPROTEING"/>
    </dbReference>
    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00224">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48670">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50058">
        <property type="entry name" value="G_PROTEIN_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="G protein gamma" evidence="11">
        <location>
            <begin position="8"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017310671.1"/>
        </source>
    </evidence>
    <sequence length="73" mass="8273" checksum="8E3250E41A58107F" modified="2017-12-20" version="1">MPVIDVDNLTDLDKAKMEVDQLKKEVKLEREKVSKCCEEVMEYIQGGMDDDPLVKGIPEEKNPFKEKGGCVIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q2I5</accession>
    <name>A0A2D0Q2I5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Mucin-5AC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">si:ch211-14k19.8</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
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    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
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        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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            <end position="987"/>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017312943.2"/>
        </source>
    </evidence>
    <sequence length="1154" mass="123975" checksum="EE1BC50F48904385" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q5L8</accession>
    <name>A0A2D0Q5L8_ICTPU</name>
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        <submittedName>
            <fullName evidence="6">Protein SGT1 homolog isoform X1</fullName>
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        <name evidence="6" type="primary">sugt1</name>
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    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
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    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
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        <text evidence="1">Belongs to the SGT1 family.</text>
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    <dbReference type="OMA" id="WIKKCEE"/>
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        <property type="component" value="Chromosome 26"/>
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    <dbReference type="GO" id="GO:0005737">
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <location>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017312996.1"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q6A8</accession>
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        <recommendedName>
            <fullName evidence="15">Galanin receptor type 1</fullName>
        </recommendedName>
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    <gene>
        <name evidence="20" type="primary">galr1b</name>
    </gene>
    <organism evidence="19 20">
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Receptor for the hormone galanin. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with GRP39 AND HTR1A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017313837.1">
        <property type="nucleotide sequence ID" value="XM_017458348.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q6A8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000025608"/>
    <dbReference type="GeneID" id="108259125"/>
    <dbReference type="KEGG" id="ipu:108259125"/>
    <dbReference type="CTD" id="561989"/>
    <dbReference type="OMA" id="CWAGATR"/>
    <dbReference type="OrthoDB" id="2132067at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004966">
        <property type="term" value="F:galanin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd15098">
        <property type="entry name" value="7tmA_Gal1_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000135">
        <property type="entry name" value="galanin receptor type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000405">
        <property type="entry name" value="Galanin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF25">
        <property type="entry name" value="GALANIN RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00663">
        <property type="entry name" value="GALANINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 16" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9 16 20" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 16" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="69"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="199"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="279"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="18">
        <location>
            <begin position="46"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00063558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00074701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017313837.1"/>
        </source>
    </evidence>
    <sequence length="346" mass="39554" checksum="B85F9C5F557D165A" modified="2017-12-20" version="1">MVLSRNETLWSEDQPDANRTELEPLSPEVVIVPVIFGCIFFLGIVGNTLVMVVIGRIKSKRSRSTTNIFILNLSIADLSFLLFCVPFQATIYSLPEWVFGAFLCKFVHYIFTLSMLVSIFTLVAMSVDRYVAVVLSKKSPCIRNRRNTLAGVCVIWAMSFVFAVPMAQHQILTNHPDAPNSSFCWEIWPESVARRTYKVTILVIGYLLPLLLITCCYTKVLYHLHKKIKNMSKKSERSKRKTAQTVLLVVAAFLFCWMPHHIIAMWVEFGDFPLNDASFAFRIMSHCLAYGNSCVNPILYAFLSENFRKACRQVFTCRLFYSPPPVKKIARIRMENFSTTHSTTNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QAZ0</accession>
    <name>A0A2D0QAZ0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Syntaxin-18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">stx18</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Syntaxin that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017315853.1">
        <property type="nucleotide sequence ID" value="XM_017460364.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QAZ0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000618"/>
    <dbReference type="GeneID" id="108260254"/>
    <dbReference type="KEGG" id="ipu:108260254"/>
    <dbReference type="CTD" id="53407"/>
    <dbReference type="OMA" id="FVFQCRE"/>
    <dbReference type="OrthoDB" id="342981at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15850">
        <property type="entry name" value="SNARE_syntaxin18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000015">
        <property type="entry name" value="Syntaxin-18, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019529">
        <property type="entry name" value="Syntaxin-18_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15959">
        <property type="entry name" value="SYNTAXIN-18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15959:SF0">
        <property type="entry name" value="SYNTAXIN-18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10496">
        <property type="entry name" value="Syntaxin-18_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="SNARE-complex protein Syntaxin-18 N-terminal" evidence="16">
        <location>
            <begin position="4"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="169"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="215"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF10496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017315853.1"/>
        </source>
    </evidence>
    <sequence length="318" mass="36606" checksum="981B099338946D9A" modified="2017-12-20" version="1">MAVDITLLFKASVKTVKTRNKAIGVGPESAKDELVRRNRPKNSFSSKAKEVISNITKLKDFLLQHRKDYVNAGSLLSSDVTRMTDCERDQIDQDAQIFMRTCSDAIKQLRTEAEKTVISAQMKQHRGAVLDLIEVYLKGVCKLYSEQRAIRVKRVVDKKRLSRLEPEQKIKVEKPQSEDTERTVPEESSEKPTCDSGVDLWEDSRVEDELSPEEIQMFEQENQRLVSEMNSLVDEVRQIEGKVVEISRLQEIFSEKVLQQETEIDNIHQLVVGATENVKEGNEDIREAIKNNAGFRVWILFFLVMCSFSLLFLDWYDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QCJ4</accession>
    <name>A0A2D0QCJ4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16 17">Uncharacterized protein LOC108260327 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16 17" type="primary">LOC108260327</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16 17" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16 17">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_047007814.1">
        <property type="nucleotide sequence ID" value="XM_047151858.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053533057.1">
        <property type="nucleotide sequence ID" value="XM_053677082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QCJ4"/>
    <dbReference type="GeneID" id="108260327"/>
    <dbReference type="KEGG" id="ipu:108260327"/>
    <dbReference type="OrthoDB" id="9898017at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042130">
        <property type="term" value="P:negative regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042102">
        <property type="term" value="P:positive regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031295">
        <property type="term" value="P:T cell costimulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051713">
        <property type="entry name" value="T-cell_Activation_Regulation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466:SF14">
        <property type="entry name" value="BUTYROPHILIN SUBFAMILY 2 MEMBER A2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466">
        <property type="entry name" value="T-LYMPHOCYTE ACTIVATION ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044573393" evidence="13">
        <location>
            <begin position="23"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="156"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="42"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="205"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_047007814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_053533057.1"/>
        </source>
    </evidence>
    <sequence length="254" mass="28613" checksum="0651E1115AB03079" modified="2017-12-20" version="1">MFCEMKHWILWLSLCHISHTRGESLCKSELLLSSNISTELQSDVLLPCNFNTTLLGSDKTADISVVWSQRNTTIHNLVEITLQGNEMFWESKRGRIKTFAKLSESGNFSILLQKVQPYDLGLYLCELFNGTGCRIAYQELHLNRWTSMNIVVSLPIWPFIAAGGGAAALILLVVLICLIKCACSNASNKHIYANSRFEKRNDKECSEEKHSSSNQHAAKARNKVDVNKPPNHSNIYANSHMEAETIYANQPHCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QDE5</accession>
    <name>A0A2D0QDE5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein LOC108260174</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC108260174</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SDA103</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017315737.1">
        <property type="nucleotide sequence ID" value="XM_017460248.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QDE5"/>
    <dbReference type="GeneID" id="108260174"/>
    <dbReference type="KEGG" id="ipu:108260174"/>
    <dbReference type="OrthoDB" id="9833817at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026238">
        <property type="entry name" value="INCA1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15142">
        <property type="entry name" value="INCA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_017315737.1"/>
        </source>
    </evidence>
    <sequence length="289" mass="32561" checksum="42C2D51491E3294D" modified="2017-12-20" version="1">MWQQWTTHGFQLYQVEPAETEESGQFLSFAKRSREVHRCSAATVCLPAPDLHLSSSLSQYGEEFWEKILRYPSEKAACYDPGCGDPLNMFYREPTEMVCTGVRGVREENLSVDTLPSPAQLMGRRKKGRGRVQRRDAAQRQAAAVTQHIGDLRRKQSAVDQMKTEKWWGSTLGYPPENSCSLDVINLTPPETPWRLDPSHTHAQYSAPSPTHLNTGTINPQVFGGCEEMMDLAPGGNPMMSFVVTGTAHREARRNWDTPCLDSPYVATPHLDTPYLHKPHSFWGFPSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QMN2</accession>
    <name>A0A2D0QMN2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Transcription factor IIIB 90 kDa subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">B-related factor 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">brf1a</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="13">TFIIIB comprises at least the TATA-binding protein (TBP) and the B-related factor 1 (BRF1/TFIIIB90). Interacts with BDP1. Interacts with MAF1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017318981.1">
        <property type="nucleotide sequence ID" value="XM_017463492.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QMN2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034706"/>
    <dbReference type="GeneID" id="108263064"/>
    <dbReference type="KEGG" id="ipu:108263064"/>
    <dbReference type="CTD" id="334402"/>
    <dbReference type="OrthoDB" id="511529at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000126">
        <property type="term" value="C:transcription factor TFIIIB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000995">
        <property type="term" value="F:RNA polymerase III general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20553">
        <property type="entry name" value="CYCLIN_TFIIIB90_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20554">
        <property type="entry name" value="CYCLIN_TFIIIB90_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000012">
        <property type="entry name" value="Putative transcription factor IIIB 90 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000002">
        <property type="entry name" value="Transcription factor IIIB 90 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transcription factor IIIB 90 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.650:FF:000001">
        <property type="entry name" value="transcription factor IIIB 90 kDa subunit isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.650">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BRF1_TBP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF4">
        <property type="entry name" value="TRANSCRIPTION FACTOR IIIB 90 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07741">
        <property type="entry name" value="BRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="19" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="17">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="373"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="429"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="489"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="546"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="637"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="489"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="505"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="644"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00031009"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00063003"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00072559"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017318981.1"/>
        </source>
    </evidence>
    <sequence length="676" mass="75398" checksum="B7C925455E1DB1D9" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QT60</accession>
    <name>A0A2D0QT60_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">B-type lectin plumieribetin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108264531</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017321623.1">
        <property type="nucleotide sequence ID" value="XM_017466134.2"/>
    </dbReference>
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    <dbReference type="GeneID" id="108264531"/>
    <dbReference type="KEGG" id="ipu:108264531"/>
    <dbReference type="OMA" id="HEIWNSA"/>
    <dbReference type="OrthoDB" id="1884773at2759"/>
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        <property type="component" value="Chromosome 4"/>
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        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Bulb-type_lectin_dom"/>
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        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017321623.1"/>
        </source>
    </evidence>
    <sequence length="152" mass="17073" checksum="86170FAA8EE9946E" modified="2017-12-20" version="1">MRVRALNSETPSACPYKPPSLITTQTEADHDSSPLPLSLRRTMSRNFLSMNDELQKGDFLLSNNRKYRAVFQEDGNFVVYGCGPVWASNTCGITDAHRLIMQEDCNLVIYTPCKPVWSTNTNRSGVKRCRLTLRDDGTLVVENDGAVVWKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QUN5</accession>
    <name>A0A2D0QUN5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mannosyltransferase</fullName>
            <ecNumber evidence="9">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">pigb</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Alpha-1,2-mannosyltransferase that catalyzes the transfer of the third mannose, via an alpha-1,2 bond, from a dolichol-phosphate-mannose (Dol-P-Man) to an alpha-D-Man-(1-&gt;6)-2-PEtn-alpha-D-Man-(1-&gt;4)-alpha-D-GlcN-(1-&gt;6)-(1-radyl,2-acyl-sn-glycero-3-phospho)-2-acyl-inositol intermediate to generate an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)-2-PEtn-alpha-D-Man-(1-&gt;4)-alpha-D-GlcN-(1-&gt;6)-(1-radyl,2-acyl-sn-glycero-3-phospho)-2-acyl-inositol (also termed H6) and participates in the nineth step of the glycosylphosphatidylinositol-anchor biosynthesis. May also add the third mannose to an alpha-D-Man-(1-&gt;6)-alpha-D-Man-(1-&gt;4)-alpha-D-GlcN-(1-&gt;6)-(1-radyl,2-acyl-sn-glycero-3-phospho)-2-acyl-inositol (also termed H3) intermediate generating an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)-alpha-D-Man-(1-&gt;4)-alpha-D-GlcN-(1-&gt;6)-(1-radyl,2-acyl-sn-glycero-3-phospho)-2-acyl-inositol (also termed H4).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Endoplasmic reticulum membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glycosyltransferase 22 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="9"/>
    <dbReference type="RefSeq" id="XP_017321446.3">
        <property type="nucleotide sequence ID" value="XM_017465957.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QUN5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000023580"/>
    <dbReference type="GeneID" id="108264437"/>
    <dbReference type="KEGG" id="ipu:108264437"/>
    <dbReference type="CTD" id="9488"/>
    <dbReference type="OMA" id="HHMVFNN"/>
    <dbReference type="OrthoDB" id="416834at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF4">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2 9 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="207"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="250"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="326"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="374"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00093333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017321446.3"/>
        </source>
    </evidence>
    <sequence length="531" mass="61747" checksum="146CBFB8BB12089C" modified="2023-06-28" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QVX5</accession>
    <name>A0A2D0QVX5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Interleukin-4 receptor subunit alpha isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC108264536</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017321635.1">
        <property type="nucleotide sequence ID" value="XM_017466146.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QVX5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000461"/>
    <dbReference type="GeneID" id="108264536"/>
    <dbReference type="KEGG" id="ipu:108264536"/>
    <dbReference type="OMA" id="CNEYCTL"/>
    <dbReference type="OrthoDB" id="8939865at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016064">
        <property type="term" value="P:immunoglobulin mediated immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037:SF42">
        <property type="entry name" value="CYTOKINE RECEPTOR COMMON SUBUNIT GAMMA ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012226374" evidence="9">
        <location>
            <begin position="22"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="254"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="317"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="319"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017321635.1"/>
        </source>
    </evidence>
    <sequence length="397" mass="45741" checksum="828BA961B8CE0CA8" modified="2017-12-20" version="1">MRCSLFFQLFVCFSHLRGTLMCLNMTDGHNYRLECLSDYLYTIRCELNMTSEPGPRDALCWLEFHYESETFECAFTSQTYSWLCVLNLYTRVEHTFMDTDIFQISLNYSFHGNNGSTVLKKDYMPVKNIQPVPPSKLMLLWKPDEAVFHWLSGYNENVMLVPFLQYQLSIHRTNKVLDVHSFQTNVSVPMSTFEPDTNYTAHLRSAPYGTSYKGVWSHWGPAIYWRTRRTKKVKLDTAATGPEADPLDMSYARVWLALFLLPLILFCYFSYTRWKRYVLNPGPAPHIGDFKCLGMLPENVGELLQKEESLQIDHLTEEPNPTQYKNMSNVNDSSGHTHITGSPRSPSTPQFSVDSKVSMSSWMQVLMSAENGSVTYSDDYCTLSHALSEHAVNTHKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QYY6</accession>
    <name>A0A2D0QYY6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="5">PPIase</shortName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ppiaa</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 5">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017323530.1">
        <property type="nucleotide sequence ID" value="XM_017468041.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QYY6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008436"/>
    <dbReference type="GeneID" id="108265559"/>
    <dbReference type="KEGG" id="ipu:108265559"/>
    <dbReference type="CTD" id="336612"/>
    <dbReference type="OMA" id="RICFELY"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001666">
        <property type="term" value="P:response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000013">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0697">Rotamase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017323530.1"/>
        </source>
    </evidence>
    <sequence length="164" mass="17586" checksum="3805486787107E8C" modified="2017-12-20" version="1">MANPRVFFDITAGGNPLGRVIMELRADVVPRTAENFRQLCTGQPGFGYKGSTFHRVIPNFMCQGGDFTNHNGTGGKSIYGHKFEDENFSLKHTGAGCLSMANAGPNTNGSQFFICTANTSWLDGKHVVFGQVVEGMEVIKKVEGYGSNSGKTSAQIVIADCGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QZS1</accession>
    <name>A0A2D0QZS1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5 6">BLOC-1 related complex subunit 6 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5 6 7" type="primary">borcs6</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4 5 6" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4 5 6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017323794.1">
        <property type="nucleotide sequence ID" value="XM_017468305.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017323795.1">
        <property type="nucleotide sequence ID" value="XM_017468306.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047011641.1">
        <property type="nucleotide sequence ID" value="XM_047155685.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047011642.1">
        <property type="nucleotide sequence ID" value="XM_047155686.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QZS1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009499"/>
    <dbReference type="GeneID" id="108265702"/>
    <dbReference type="KEGG" id="ipu:108265702"/>
    <dbReference type="CTD" id="54785"/>
    <dbReference type="OMA" id="MQPIQTL"/>
    <dbReference type="OrthoDB" id="21270at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099078">
        <property type="term" value="C:BORC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032418">
        <property type="term" value="P:lysosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019314">
        <property type="entry name" value="BORCS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046465">
        <property type="entry name" value="BORCS6_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13440">
        <property type="entry name" value="BLOC-1 RELATED COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13440:SF7">
        <property type="entry name" value="BLOC-1 RELATED COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10157">
        <property type="entry name" value="BORCS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BLOC-1-related complex subunit 6 C-terminal helix" evidence="2">
        <location>
            <begin position="341"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017323794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017323795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_047011641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_047011642.1"/>
        </source>
    </evidence>
    <sequence length="444" mass="48466" checksum="16FC112081EE9676" modified="2017-12-20" version="1">MSHSSGIGHDVQEETDGTDSPDSPEVCNWTAANGSASHMQKCRDGVACLEKDKQEGTPETHAHPRHSMDPAYRQIHTNSAPHTNTYACEDGPTHTDEYTPHSETVTKDSPYMETASMPHTQASDGWGGVEEEEEEEVRSQDDERDGEEVENREENQQTADFTVQPVKETTSEDSTTSQMPPTEPVNCISYDDGHLSASTPPDQAPPLVPAPPPSSVPPPLLPAPDDWPRPTHVMAEVRVRSAPGRERVVRGMQDSKSLDGISEACGSGMRAAAGEGRRATISSALELEGTVSRDGDLTHFIAKNLEQKIKMSSRPSLDTDSDCSGPVVRGRGSLRRPADIPPIDPSVLLDLHKHTQEVAQSVEMMLRSLNGTIQNMTALSVGYIQTYRDSVDSLGESVDMSIKGMYTLMARCEELDRSMQPIQTLAAQIRDIKRTLDTLEAICK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R529</accession>
    <name>A0A2D0R529_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DDB1- and CUL4-associated factor 6 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">dcaf6</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017325156.1">
        <property type="nucleotide sequence ID" value="XM_017469667.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R529"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033985"/>
    <dbReference type="GeneID" id="108266419"/>
    <dbReference type="KEGG" id="ipu:108266419"/>
    <dbReference type="CTD" id="55827"/>
    <dbReference type="OrthoDB" id="4869960at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000045">
        <property type="entry name" value="DDB1- and CUL4-associated factor 6 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045151">
        <property type="entry name" value="DCAF8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15574:SF39">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15574">
        <property type="entry name" value="WD REPEAT DOMAIN-CONTAINING FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="44"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="899"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="284"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="376"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="529"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="284"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="308"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="398"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="490"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="535"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="560"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="578"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="602"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="631"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="663"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="724"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="735"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="769"/>
            <end position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017325156.1"/>
        </source>
    </evidence>
    <sequence length="994" mass="107128" checksum="B842CC86F7289BCC" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R875</accession>
    <name>A0A2D0R875_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kinesin light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">klc2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Kinesin is a microtubule-associated force-producing protein that play a role in organelle transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Oligomeric complex composed of two heavy chains and two light chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
            <location evidence="1 11">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the kinesin light chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017326779.1">
        <property type="nucleotide sequence ID" value="XM_017471290.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R875"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013570"/>
    <dbReference type="GeneID" id="108267279"/>
    <dbReference type="KEGG" id="ipu:108267279"/>
    <dbReference type="CTD" id="64837"/>
    <dbReference type="OMA" id="DAECLYK"/>
    <dbReference type="OrthoDB" id="413723at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000003">
        <property type="entry name" value="kinesin light chain isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002151">
        <property type="entry name" value="Kinesin_light"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015792">
        <property type="entry name" value="Kinesin_light_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783">
        <property type="entry name" value="KINESIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783:SF2">
        <property type="entry name" value="KINESIN LIGHT CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00381">
        <property type="entry name" value="KINESINLIGHT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01160">
        <property type="entry name" value="KINESIN_LIGHT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 10" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="239"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="281"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="323"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="144"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="496"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="85"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="532"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="542"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="589"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017326779.1"/>
        </source>
    </evidence>
    <sequence length="616" mass="68514" checksum="A0CB644B1C879FCF" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R9U2</accession>
    <name>A0A2D0R9U2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Kv channel-interacting protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">kcnip4a</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the recoverin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017327339.1">
        <property type="nucleotide sequence ID" value="XM_017471850.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R9U2"/>
    <dbReference type="GeneID" id="108267605"/>
    <dbReference type="CTD" id="100536963"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005267">
        <property type="term" value="F:potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000043">
        <property type="entry name" value="Kv channel-interacting protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF30">
        <property type="entry name" value="KV CHANNEL-INTERACTING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="19" id="KW-0407">Ion channel</keyword>
    <keyword evidence="16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0630">Potassium</keyword>
    <keyword evidence="12" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="8" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="14" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="domain" description="EF-hand" evidence="21">
        <location>
            <begin position="99"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="21">
        <location>
            <begin position="135"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="21">
        <location>
            <begin position="183"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00040738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017327339.1"/>
        </source>
    </evidence>
    <sequence length="229" mass="26581" checksum="2B34B830CE7BEE94" modified="2017-12-20" version="1">MNLEGLEMIAVLVVVVLFVKVLEQFGLLEASYDDSAEDELELSAVRHRPEALEQLEAQTRFSRKELQILYRGFKNECPSGVVNEDTFKDIYSQFFPQGDASTYAHFLFNAFDTDHNGAVSFEDFVMGLSILLRGTVQEKLNWAFNLYDINKDGYITKEEMLDIMKAIYDMMGKWTYPILKEETPKQHVEIFFQKMDKNKDGVVTIDEFIDCCQNDENIMRSMQLFENVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RAK2</accession>
    <name>A0A2D0RAK2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Arf-GAP domain and FG repeat-containing protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">agfg2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017327136.1">
        <property type="nucleotide sequence ID" value="XM_017471647.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RAK2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000001620"/>
    <dbReference type="GeneID" id="108267520"/>
    <dbReference type="KEGG" id="ipu:108267520"/>
    <dbReference type="CTD" id="3268"/>
    <dbReference type="OrthoDB" id="6036at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001675">
        <property type="term" value="P:acrosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007289">
        <property type="term" value="P:spermatid nucleus differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08838">
        <property type="entry name" value="ArfGap_AGFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000005">
        <property type="entry name" value="Arf-GAP domain and FG repeat-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052248">
        <property type="entry name" value="Arf-GAP_FG-repeat_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46134:SF6">
        <property type="entry name" value="ARF-GAP DOMAIN AND FG REPEAT-CONTAINING PROTEIN 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46134">
        <property type="entry name" value="DRONGO, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="7">
        <location>
            <begin position="14"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="312"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="369"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="143"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="312"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="327"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="337"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="369"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="395"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="412"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017327136.1"/>
        </source>
    </evidence>
    <sequence length="467" mass="50229" checksum="B28E3C279BE7912C" modified="2017-12-20" version="1">MSNRKHRDNQEICARKVRELAQTGPNKHCFECGQPGVTYTDITVGCFVCTSCSGMLRGLNPPHRVKSISMTTFSQQEVEFLQNHGNEVGKRMWLCTFDPKTDGSFDPRDSQKFKEFLQDKYERKKWHFSKSKGRRDVEPAWSTGVQAPAQTPSGNPLNPAARTSRALSQAQLPSWDRVPAVSPADVRTEVFTARPTRSQSFRDHTIKDSLLSGMERQRPGTIYPGMGPQSRLPSFPALPRPSASSTFKNSFTLGRTVSSGGGAPFRTVPKSLSLDFGGLGSTPSALSHQAPPLVSQDRYAAVSQLDKAFSDTPTTVASTAPSSGPPQYSTLFGNRLSSSSTPASSPGVPEVTSASQTFANFPNPFCSTASSSQPALSPSNPFNSTVTGDSSTLVSPSPSAAFPHSASFPAPTSQNAFPHDPANNQETNGFSSFPALESTSKVQRSMSVNPFTGTVYPTRGTSLNPFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RBQ6</accession>
    <name>A0A2D0RBQ6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="5">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">phlpp1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 21">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017327571.1">
        <property type="nucleotide sequence ID" value="XM_017472082.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000018848"/>
    <dbReference type="GeneID" id="108267724"/>
    <dbReference type="KEGG" id="ipu:108267724"/>
    <dbReference type="CTD" id="23239"/>
    <dbReference type="OrthoDB" id="1394818at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000173">
        <property type="entry name" value="PH domain and leucine rich repeat protein phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000027">
        <property type="entry name" value="PH domain and leucine rich repeat protein phosphatase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000003">
        <property type="entry name" value="PH domain and leucine-rich repeat protein phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055071">
        <property type="entry name" value="RA_PHLPP-like"/>
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    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF43">
        <property type="entry name" value="PH DOMAIN AND LEUCINE RICH REPEAT PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23010">
        <property type="entry name" value="RA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="1100"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="100"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="242"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="311"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1368"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1475"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1513"/>
            <end position="1648"/>
        </location>
    </feature>
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        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="260"/>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="285"/>
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    </feature>
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        <location>
            <begin position="382"/>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1391"/>
            <end position="1411"/>
        </location>
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    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="1513"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1540"/>
            <end position="1549"/>
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        <location>
            <begin position="1550"/>
            <end position="1563"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1621"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017327571.1"/>
        </source>
    </evidence>
    <sequence length="1648" mass="180004" checksum="D3ACADCAD49F50D1" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RC07</accession>
    <name>A0A2D0RC07_ICTPU</name>
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        <submittedName>
            <fullName evidence="2 3">UPF0687 protein C20orf27 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2 3" type="primary">LOC108267974</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SDA103</strain>
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    </reference>
    <reference evidence="2 3" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2 3">Blood</tissue>
        </source>
    </reference>
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        <property type="component" value="Chromosome 7"/>
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        <property type="entry name" value="ADISSP"/>
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        <property type="entry name" value="ADIPOSE-SECRETED SIGNALING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SI:CH211-74F19.2"/>
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        <property type="entry name" value="DUF4517"/>
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    </dbReference>
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            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_017328077.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053537920.1"/>
        </source>
    </evidence>
    <sequence length="176" mass="19172" checksum="7CAF2FF3F44E1B86" modified="2023-06-28" version="2">MATAKKASSKAGGIRFAEANSAAAAAAHSHVHFDEKLHDSVVMVIPESDGNFLVKVGFLKTQHRYEIVFMLPEVPGLGKDVCPAPVPNPYLQIKDLTAAPSGGLKVTCEYVAHKEGVLCEEMLILSESKEDVCLKVKVHARVMDRHHGTPMLLEGVRCVGAELEYDSEQSDWQGFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RC38</accession>
    <name>A0A2D0RC38_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ADP-ribosylation factor-like protein 14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ADP-ribosylation factor 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">arl14</name>
    </gene>
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        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">GTPase that recruits MYO1E to MHC class II-containing vesicles via the effector protein ARL14EP and hence controls the movement of these vesicles along the actin cytoskeleton in dendritic cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with ARL14EP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017327440.1">
        <property type="nucleotide sequence ID" value="XM_017471951.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RC38"/>
    <dbReference type="GeneID" id="108267661"/>
    <dbReference type="KEGG" id="ipu:108267661"/>
    <dbReference type="CTD" id="80117"/>
    <dbReference type="OrthoDB" id="6228084at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000412">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="17"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="123"/>
            <end position="126"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00072405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00077764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017327440.1"/>
        </source>
    </evidence>
    <sequence length="196" mass="22570" checksum="2A470175EB6FF14A" modified="2017-12-20" version="1">MGLKGSKLPDARILLLGLDGAGKSTLLFKLKYNELFQTAPTIGFNVEMMELKRNRRKITLTVWDVGGQEKMRTHWRNYYQDTAGLLFVVDCWDRRRLDEAKRELERVLKNEHLRGLPVVFLANKQDKEGALTAIELTETFNLRKTCSDRDWFVQPCSGKTGAGLPEGFRRLAHLVETTSNNNIKETVKNFRKSIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RC96</accession>
    <name>A0A2D0RC96_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Clusterin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">clul1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the clusterin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017327746.1">
        <property type="nucleotide sequence ID" value="XM_017472257.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RC96"/>
    <dbReference type="GeneID" id="108267820"/>
    <dbReference type="CTD" id="27098"/>
    <dbReference type="OrthoDB" id="9894485at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000753">
        <property type="entry name" value="Clusterin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016015">
        <property type="entry name" value="Clusterin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016014">
        <property type="entry name" value="Clusterin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10970">
        <property type="entry name" value="CLUSTERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10970:SF2">
        <property type="entry name" value="CLUSTERIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01093">
        <property type="entry name" value="Clusterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00035">
        <property type="entry name" value="CLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00030">
        <property type="entry name" value="CLb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013311169" description="Clusterin" evidence="10">
        <location>
            <begin position="20"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Clusterin N-terminal" evidence="11">
        <location>
            <begin position="25"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Clusterin C-terminal" evidence="12">
        <location>
            <begin position="234"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="62"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017327746.1"/>
        </source>
    </evidence>
    <sequence length="458" mass="51882" checksum="683FFC1FA76ED1A7" modified="2017-12-20" version="1">MRVLLFSLALLALLKVFHSAPSHAPPAVSTETVKRLSLEGEKLVDEEVQRALYGIKQMKEVMASNEKKHKQLIKTLENSREKKKEVAEMAQHVVKKLDEAEQQCKETLKSLWDECQPCLQEACKSFYNTTCRRGFATFANKLEGFFQRVNTHFGVPSTREELTVNKSAEMVDLDLRRIEDSFNDLVSKVDILVNRSMALVFRFHSKLDETLQQVFAPKPQEDKEIEAPDSDFMQGIGVGLKEVLESFVDFTSSVMEEFESVITQAFDDLRGVIEEEETEQEKHLLPGFIQNRTLCRDLRRQSAECWQLQNKCQACQGPLFSECPSMRELHIELNEVSGLLEVAKEQYEEVLGIAERHTVDTISWINNMAGQFGWVAELADGGVTNENFFSVTTVENEEAVHVPGEGMNTPAADTKVEVNILNAPPLIFTVPGSLQPQDPAFIQYIANEALGFYKQKIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RCG0</accession>
    <name>A0A2D0RCG0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Matrix metalloproteinase-14</fullName>
            <ecNumber evidence="6">3.4.24.80</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="34 35" type="primary">mmp14b</name>
    </gene>
    <organism evidence="33 35">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="33">SDA103</strain>
        </source>
    </reference>
    <reference evidence="34 35" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="34 35">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endopeptidase activity. Activates progelatinase A by cleavage of the propeptide at 37-Asn-|-Leu-38. Other bonds hydrolyzed include 35-Gly-|-Ile-36 in the propeptide of collagenase 3, and 341-Asn-|-Phe-342, 441-Asp-|-Leu-442 and 354-Gln-|-Thr-355 in the aggrecan interglobular domain.</text>
            <dbReference type="EC" id="3.4.24.80"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="26">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="26">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="26">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="26">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Melanosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.80" evidence="6"/>
    <dbReference type="RefSeq" id="XP_017327770.1">
        <property type="nucleotide sequence ID" value="XM_017472281.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017327771.1">
        <property type="nucleotide sequence ID" value="XM_017472282.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RCG0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009733"/>
    <dbReference type="GeneID" id="108267832"/>
    <dbReference type="KEGG" id="ipu:108267832"/>
    <dbReference type="CTD" id="566945"/>
    <dbReference type="GeneTree" id="ENSGT00940000157808"/>
    <dbReference type="OrthoDB" id="406838at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048870">
        <property type="term" value="P:cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031638">
        <property type="term" value="P:zymogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000005">
        <property type="entry name" value="Matrix metallopeptidase 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.110.10.10:FF:000001">
        <property type="entry name" value="Matrix metallopeptidase 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.101.10:FF:000002">
        <property type="entry name" value="Matrix metalloproteinase-14 preproprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000585">
        <property type="entry name" value="Hemopexin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018486">
        <property type="entry name" value="Hemopexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021805">
        <property type="entry name" value="Pept_M10A_metallopeptidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021158">
        <property type="entry name" value="Pept_M10A_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF24">
        <property type="entry name" value="MATRIX METALLOPROTEINASE-14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11857">
        <property type="entry name" value="DUF3377"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001191">
        <property type="entry name" value="Peptidase_M10A_matrix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00546">
        <property type="entry name" value="CYSTEINE_SWITCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00024">
        <property type="entry name" value="HEMOPEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51642">
        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18 26" id="KW-0106">Calcium</keyword>
    <keyword evidence="11" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="23" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="21 30" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 25" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20 34 35" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0645">Protease</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0677">Repeat</keyword>
    <keyword evidence="14 31" id="KW-0732">Signal</keyword>
    <keyword evidence="12 30" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="19 30" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="17 25" id="KW-0862">Zinc</keyword>
    <keyword evidence="22" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="31">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013510315" description="Matrix metalloproteinase-14" evidence="31">
        <location>
            <begin position="23"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="560"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="32">
        <location>
            <begin position="111"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="28">
        <location>
            <begin position="316"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="28">
        <location>
            <begin position="365"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="28">
        <location>
            <begin position="412"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="28">
        <location>
            <begin position="461"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="273"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="513"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="283"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="29">
        <location>
            <begin position="515"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="active site" evidence="24">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="26">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="369"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphotyrosine; by PKDCC" evidence="27">
        <location>
            <position position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="RefSeq" id="XP_017327770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="RefSeq" id="XP_017327771.1"/>
        </source>
    </evidence>
    <sequence length="603" mass="68661" checksum="E5369A657AD91A21" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RIU2</accession>
    <name>A0A2D0RIU2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11 12">Cryptochrome-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 11 12" type="primary">cry2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11 12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11 12">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="5">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA photolyase class-1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017330449.1">
        <property type="nucleotide sequence ID" value="XM_017474960.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017330450.1">
        <property type="nucleotide sequence ID" value="XM_017474961.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053538181.1">
        <property type="nucleotide sequence ID" value="XM_053682206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RIU2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013259"/>
    <dbReference type="GeneID" id="108269229"/>
    <dbReference type="KEGG" id="ipu:108269229"/>
    <dbReference type="CTD" id="1408"/>
    <dbReference type="OMA" id="CPMAKEE"/>
    <dbReference type="OrthoDB" id="435881at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043153">
        <property type="term" value="P:entrainment of circadian clock by photoperiod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.579.10:FF:000001">
        <property type="entry name" value="Cryptochrome 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.579.10">
        <property type="entry name" value="DNA Cyclobutane Dipyrimidine Photolyase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036134">
        <property type="entry name" value="Crypto/Photolyase_FAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036155">
        <property type="entry name" value="Crypto/Photolyase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005101">
        <property type="entry name" value="Cryptochr/Photolyase_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002081">
        <property type="entry name" value="Cryptochrome/DNA_photolyase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006050">
        <property type="entry name" value="DNA_photolyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455">
        <property type="entry name" value="CRYPTOCHROME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455:SF15">
        <property type="entry name" value="CRYPTOCHROME-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00875">
        <property type="entry name" value="DNA_photolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03441">
        <property type="entry name" value="FAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48173">
        <property type="entry name" value="Cryptochrome/photolyase FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52425">
        <property type="entry name" value="Cryptochrome/photolyase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51645">
        <property type="entry name" value="PHR_CRY_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0157">Chromophore</keyword>
    <keyword evidence="3 5" id="KW-0274">FAD</keyword>
    <keyword evidence="2 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Photolyase/cryptochrome alpha/beta" evidence="8">
        <location>
            <begin position="3"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="508"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="513"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="569"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="289"/>
            <end position="296"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="387"/>
            <end position="389"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="6">
        <location>
            <position position="320"/>
        </location>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="6">
        <location>
            <position position="374"/>
        </location>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="6">
        <location>
            <position position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602081-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602081-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017330449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017330450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053538181.1"/>
        </source>
    </evidence>
    <sequence length="595" mass="68329" checksum="0FE8C0783926DE56" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RLS9</accession>
    <name>A0A2D0RLS9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Proton-coupled zinc antiporter SLC30A1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">slc30a1b</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017331101.1">
        <property type="nucleotide sequence ID" value="XM_017475612.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RLS9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035193"/>
    <dbReference type="GeneID" id="108269644"/>
    <dbReference type="KEGG" id="ipu:108269644"/>
    <dbReference type="CTD" id="100038803"/>
    <dbReference type="OMA" id="THDASQM"/>
    <dbReference type="OrthoDB" id="29444at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019855">
        <property type="term" value="F:calcium channel inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010312">
        <property type="term" value="P:detoxification of zinc ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097325">
        <property type="term" value="P:melanocyte proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45820">
        <property type="entry name" value="FI23527P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45820:SF1">
        <property type="entry name" value="PROTON-COUPLED ZINC ANTIPORTER SLC30A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="217"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="9">
        <location>
            <begin position="12"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="10">
        <location>
            <begin position="323"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017331101.1"/>
        </source>
    </evidence>
    <sequence length="470" mass="51781" checksum="D2883983575031D2" modified="2017-12-20" version="1">MALEPKQARLLCMLCLTCVFFVVEVVVSRVTGSLAMLSDSFHMLSDVIALLVALLAVRLADQTAATSLNTFGWIRAEVMGALVNAVFLTALCFTILLEAVERYTEPHEIEKPTVVVWVGVAGLVVNVLGLLLFHGYAGHGHSHSTSNRRYEKSETSSGEAYDLLVKSQRPGRGDHAVQVEADIVSKHNLEIPVNGNISPEKLEHDLDSSSQLNMRGVFLHVLGDAAGSVIVVINALIFTFVWRTCDNQEICINPCLSSYCSDHWPVNNTHLVHSNGTSAVAVAGPCWVLYLDPTLCVILVAILLYTTFPLLKESTLILLQSVPKQIDMQRLDKKLRSLEGVLAVHELHIWQLAGSRIIATAHVKCHDPASYMDTAKRIKNLFHEEGIHATTVQPEFSDDKELCVSECELPCYNQCTLKLCCDMSWKSRSESVLGCTYVSSSENTPQRSTASCNIQQNSEVFLQTEVESTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RMY4</accession>
    <name>A0A2D0RMY4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GPN-loop GTPase</fullName>
            <ecNumber evidence="11">3.6.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">gpn1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Small GTPase required for proper nuclear import of RNA polymerase II (RNAPII). May act at an RNAP assembly step prior to nuclear import. Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding proteins, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. May be involved in nuclear localization of XPA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Binds to RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterodimer with GPN3. Binds to RNA polymerase II (RNAPII). Interacts directly with RNAPII subunits RPB4 and RPB7 and the CTD of RPB1. Interacts with XPA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_017331300.1">
        <property type="nucleotide sequence ID" value="XM_017475811.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RMY4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000017577"/>
    <dbReference type="GeneID" id="108269792"/>
    <dbReference type="KEGG" id="ipu:108269792"/>
    <dbReference type="CTD" id="11321"/>
    <dbReference type="OMA" id="MIIVFNK"/>
    <dbReference type="OrthoDB" id="243313at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17870">
        <property type="entry name" value="GPN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000696">
        <property type="entry name" value="GPN-loop GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030230">
        <property type="entry name" value="Gpn1/Npa3/XAB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF8">
        <property type="entry name" value="GPN-LOOP GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="41"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="327"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="352"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="365"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017331300.1"/>
        </source>
    </evidence>
    <sequence length="398" mass="44597" checksum="FBB26D8510717A74" modified="2017-12-20" version="1">MATAQVESDGAAGGTEQNDGKGDENPETSSGKPSGNNAEQKPVCLIVLGMAGSGKTTFVQRLTAYLHSKKSPPYVINLDPAVHEVPFPANIDIRDTVNYKEVMKQYGLGPNGGIVTSLNLFATRFDQVMKFIEKKQQNHQYVLIDTPGQIEVFTWSASGTIITEALASSFPCVVMYVMDTSRSVNPVTFMSNMLYACSILYKTKLPFIVVMNKTDIIDHRFAVEWMQDFEVFQDALNQETSYVSNLTRSMSLVLDEFYTNLRVVGVSAVTGTGLDELFVQVTDAAKEYETEYRPEYERLHRELAEAQCKKQQEQLERLRKDMGEVSMETGVQPDVPDLSGPSDLIFTRGNPDEDEEDMDSDTDDIDHHATEESKEAKSFGNFLKERKEVVQVRNRKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RNU9</accession>
    <name>A0A2D0RNU9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">AT-rich interactive domain-containing protein 1A isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">arid1aa</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017331630.1">
        <property type="nucleotide sequence ID" value="XM_017476141.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000025039"/>
    <dbReference type="GeneID" id="108269953"/>
    <dbReference type="KEGG" id="ipu:108269953"/>
    <dbReference type="CTD" id="100141358"/>
    <dbReference type="OMA" id="DPYQANS"/>
    <dbReference type="OrthoDB" id="8709537at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
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<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
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        <name evidence="8" type="primary">fbxl8</name>
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        <name type="scientific">Ictalurus punctatus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="8">Blood</tissue>
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        <text evidence="1">Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.</text>
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        <text evidence="3">Directly interacts with SKP1 and CUL1.</text>
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        </source>
    </evidence>
    <sequence length="367" mass="41433" checksum="455F04A79142A0A2" modified="2017-12-20" version="1">MELPEEILAHIFSFLPLKDKGNAFQVCKTWSNSLTCSSAWTHTEIRCESEDCVPQRFSDLLPCIRHLKLIVSVAESAHRETGLWVLQQVLSRGDGRLRRLCISCLGNPPLFYAGQDLLQGLVDVLTNGSSLMALDLRGVPFTLSDSFVKSVATLCPALRSLFINNGSLVCGVVSETIRDVLKHCPALNTIGLFQASVSHDVLRDLLQPQRTDLKLLELRCERSLKYVTPLCDQVWEDMRRRHPGLRVDLELDHTLPELQVPSVLQPSIPVRQLRLHTWTWLLDEIRTVAQSYAGTLEVLELQTTASPELNTALVDLATQCTKLREVHCYCVVSQNVIEAFCSLCPDLHRYTLKTRKELHPWSCTTLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RX12</accession>
    <name>A0A2D0RX12_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Follistatin-related protein 3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">fstl3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017334490.1">
        <property type="nucleotide sequence ID" value="XM_017479001.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RX12"/>
    <dbReference type="GeneID" id="108271412"/>
    <dbReference type="KEGG" id="ipu:108271412"/>
    <dbReference type="CTD" id="10272"/>
    <dbReference type="OrthoDB" id="6614329at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005518">
        <property type="term" value="F:collagen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050840">
        <property type="term" value="F:extracellular matrix binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.30:FF:000028">
        <property type="entry name" value="Follistatin-related protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000021">
        <property type="entry name" value="follistatin-related protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.290.10">
        <property type="entry name" value="TGF-beta binding (TB) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003645">
        <property type="entry name" value="Fol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017878">
        <property type="entry name" value="TB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036773">
        <property type="entry name" value="TB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13866:SF29">
        <property type="entry name" value="FOLLISTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13866">
        <property type="entry name" value="SPARC OSTEONECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21333">
        <property type="entry name" value="FST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00274">
        <property type="entry name" value="FOLN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57581">
        <property type="entry name" value="TB module/8-cys domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51364">
        <property type="entry name" value="TB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012722861" evidence="5">
        <location>
            <begin position="21"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="6">
        <location>
            <begin position="27"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="7">
        <location>
            <begin position="111"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="7">
        <location>
            <begin position="184"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017334490.1"/>
        </source>
    </evidence>
    <sequence length="246" mass="26746" checksum="4D4B7E9780FF1181" modified="2017-12-20" version="1">MNLFSALHCVVLISLCGTLAKHRANAGICWLQQGQEQRCDMVLIRGVSREECCAGGRLDTAWSNTSLPINEVSLLGFLGIVSCKPCKETCEGVKCGSGKVCRMKGGRPQCICSPDCSNISRKHAVCGSDGTTYKDECALLMSRCRGHPDLEIMYQGECKKSCSNVVCPGTHTCVTDQTNSAHCVMCRTAQCPMPMLSGQTICGNNNITYPSACHLRRATCFFGRSIGVRHYGHCRSKEGSEENLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RZ97</accession>
    <name>A0A2D0RZ97_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Voltage-dependent calcium channel subunit alpha-2/delta-2a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">cacna2d2a</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calcium channel subunit alpha-2/delta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017335511.1">
        <property type="nucleotide sequence ID" value="XM_017480022.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RZ97"/>
    <dbReference type="GeneID" id="108271986"/>
    <dbReference type="KEGG" id="ipu:108271986"/>
    <dbReference type="CTD" id="541376"/>
    <dbReference type="OrthoDB" id="10054666at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12912">
        <property type="entry name" value="PDC2_MCP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000014">
        <property type="entry name" value="voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000006">
        <property type="entry name" value="voltage-dependent calcium channel subunit alpha-2/delta-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051173">
        <property type="entry name" value="Ca_channel_alpha-2/delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013680">
        <property type="entry name" value="VDCC_a2/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013608">
        <property type="entry name" value="VWA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL SUBUNIT ALPHA-2/DELTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166:SF7">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL SUBUNIT ALPHA-2_DELTA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08473">
        <property type="entry name" value="VGCC_alpha2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08399">
        <property type="entry name" value="VWA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013084666" evidence="18">
        <location>
            <begin position="32"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="19">
        <location>
            <begin position="260"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017335511.1"/>
        </source>
    </evidence>
    <sequence length="1110" mass="125870" checksum="19A92D41A6421498" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S0Q4</accession>
    <name>A0A2D0S0Q4_ICTPU</name>
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        <submittedName>
            <fullName evidence="3">Fidgetin-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">si:dkey-195m11.8</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017336021.1">
        <property type="nucleotide sequence ID" value="XM_017480532.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S0Q4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029690"/>
    <dbReference type="GeneID" id="108272232"/>
    <dbReference type="KEGG" id="ipu:108272232"/>
    <dbReference type="OMA" id="HWSSEWA"/>
    <dbReference type="OrthoDB" id="8803010at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017336021.1"/>
        </source>
    </evidence>
    <sequence length="271" mass="29901" checksum="FA7BEA5A93E45541" modified="2017-12-20" version="1">MHWSSEWAEQHYDITSTTSPPAHPKPVSYPQPSHPAFTGYSDDISALSASTLLKRYAERYSFTPVYPEPGAFRRSEPGQDAWSMGYNADGIDGIKGSVASASNLSEQFGNGAISQDYTSTYSGSHLYPRPTYLHQPAFALPPTYQTPAPVYTYPPGLTNPSSPSLLPPLSSGLQPTHSTQVLKRSEQFQEHNKTFGFDLNKSTRVSPYTARDNSEHPISNGIPNSSADLQSFRPDRILPQPVMKVDLVRKSSYLQPSETASYNGAEQYTYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S252</accession>
    <name>A0A2D0S252_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcription factor Sox-21-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">sox21a</name>
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    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017336526.1">
        <property type="nucleotide sequence ID" value="XM_017481037.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S252"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000015500"/>
    <dbReference type="GeneID" id="108272561"/>
    <dbReference type="KEGG" id="ipu:108272561"/>
    <dbReference type="CTD" id="30543"/>
    <dbReference type="OrthoDB" id="6247875at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01388">
        <property type="entry name" value="HMG-box_SoxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000002">
        <property type="entry name" value="transcription factor Sox-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050140">
        <property type="entry name" value="SRY-related_HMG-box_TF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270:SF327">
        <property type="entry name" value="PROTEIN CBG16280"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270">
        <property type="entry name" value="SOX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="5">
        <location>
            <begin position="8"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="4">
        <location>
            <begin position="8"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017336526.1"/>
        </source>
    </evidence>
    <sequence length="225" mass="25310" checksum="F68DF6DDAEF0866E" modified="2017-12-20" version="1">MSKQTDRVKRPMNAFMVWSRAQRRKMALDNPKMHNSEISKRLGGEWKLLSDSEKRPFIDEAKRLRAVHMKEHPDYKYRPRRKPKHSVKKHSYPCNVGYIFGEQDAPKGLLTSPEKTCAAVAAARALLSHSVPTTPYPYLDIHSKTSHLTATSFTHHSLGYSRHVVAFPRMGILGATHMHPSPGNPAQTMPCNCPAWTSAPPPLAYVVLPGMCKTLHEPYSCCSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S6C3</accession>
    <name>A0A2D0S6C3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Dynein axonemal intermediate chain 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">dnai2b</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein intermediate chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017338272.1">
        <property type="nucleotide sequence ID" value="XM_017482783.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S6C3"/>
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        <source>
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        </source>
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    <sequence length="584" mass="67236" checksum="F9F35333EE583CEF" modified="2017-12-20" version="1"> </sequence>
</entry>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
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                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">SDA103</strain>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017337970.1"/>
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    <sequence length="188" mass="21415" checksum="7C86398A1EB4C575" modified="2017-12-20" version="1">MVYTVMADVAEIESVKNYQIAFEKIDLNTSRYPYCIVWTPIPVLSWLFPFIGHMGICTSAGVIRDFAGPYFVSEDNMAFGRPTKYWMLDVSKVYASGSNAWDTAVHDASEEYKHRMHNLCCDNCHSHVAMALNLMHYDNSTSWNMVNLCLLALINGKHVSWAGFLKTWLPFLILCSVIVSLALYINLR</sequence>
</entry>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
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                <person name="Sonstegard T."/>
                <person name="Feng J."/>
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                <person name="Duke M.V."/>
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                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
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                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="2">Blood</tissue>
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    </dbReference>
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        <property type="entry name" value="ARMC7"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46263:SF1">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_017338998.1"/>
        </source>
    </evidence>
    <sequence length="198" mass="22149" checksum="297D16EAC3CE9AEF" modified="2017-12-20" version="1">MFNRWCSTGMNRFCASDRLEYLQGLVTEFQDTDSEEAKEQILANLANFAYDPRSMEALRMLQVTELFLDMLTEENENFVEFGIGGLCNLSMDRECRDQILQSGGIPLVTGCLSSHRDETVLSAITTLMNLTTAASRSQTTDSAVVQSMLRFSLTQNPRLSNLATVFLQDYCTQDQVDKARELLQGHSQSAVGIPLPKD</sequence>
</entry>
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    <accession>A0A2D0S8L1</accession>
    <name>A0A2D0S8L1_ICTPU</name>
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        <submittedName>
            <fullName evidence="9">Uncharacterized protein si:ch211-194k22.8 isoform X1</fullName>
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    <gene>
        <name evidence="9" type="primary">si:ch211-194k22.8</name>
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    <organism evidence="8 9">
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
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            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017338570.1">
        <property type="nucleotide sequence ID" value="XM_017483081.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S8L1"/>
    <dbReference type="GeneID" id="108273677"/>
    <dbReference type="KEGG" id="ipu:108273677"/>
    <dbReference type="OrthoDB" id="8858716at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045746">
        <property type="term" value="P:negative regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045666">
        <property type="term" value="P:positive regulation of neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2590">
        <property type="entry name" value="BEN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018379">
        <property type="entry name" value="BEN_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037496">
        <property type="entry name" value="BEND6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35346">
        <property type="entry name" value="BEN DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35346:SF1">
        <property type="entry name" value="BEN DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10523">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01025">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51457">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BEN" evidence="7">
        <location>
            <begin position="194"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="288"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="303"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017338570.1"/>
        </source>
    </evidence>
    <sequence length="327" mass="36994" checksum="090DD7BA9D1DC759" modified="2017-12-20" version="1">MRRKTKAKRILDYENEEQDEKEEQKLDEFYHGLQKRRKQTDAAKERAEENSPAAAGLQKLDMRGYGFDCSPTGRFDEGHPTAEQEAEYLQSYSKMELIAMVLCMQREMESLKEQLRCLTACGKLAKNLEALIERTQMLPNGDKKTSLPVPSSPMTEPVEVDALMPAMPYASGVMNGQWVKQETLTQKSNEVSHRNGLFTEFITPELLERCNTGTTAQKLTNDLLRGLYERDCLASHSISGVVYNKRGQPKPALPTEEVQAILRTVQYFFPGKTDAEIKGYIRQKLQNEAKRLRKKPPLSGPEPRALSLSSPRSSSHQLDLIGQKSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SD83</accession>
    <name>A0A2D0SD83_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein misato homolog 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">msto1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the regulation of mitochondrial distribution and morphology. Required for mitochondrial fusion and mitochondrial network formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the misato family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017340682.1">
        <property type="nucleotide sequence ID" value="XM_017485193.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SD83"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034121"/>
    <dbReference type="GeneID" id="108274805"/>
    <dbReference type="KEGG" id="ipu:108274805"/>
    <dbReference type="CTD" id="55154"/>
    <dbReference type="OMA" id="RMAAYGC"/>
    <dbReference type="OrthoDB" id="271881at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06060">
        <property type="entry name" value="misato"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049942">
        <property type="entry name" value="DML1/Misato"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029209">
        <property type="entry name" value="DML1/Misato_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019605">
        <property type="entry name" value="Misato_II_tubulin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13391">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION REGULATOR MISATO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13391:SF0">
        <property type="entry name" value="PROTEIN MISATO HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10644">
        <property type="entry name" value="Misat_Tub_SegII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14881">
        <property type="entry name" value="Tubulin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Misato Segment II tubulin-like" evidence="8">
        <location>
            <begin position="6"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="DML1/Misato tubulin" evidence="9">
        <location>
            <begin position="167"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017340682.1"/>
        </source>
    </evidence>
    <sequence length="589" mass="64694" checksum="F1B6644B3E0D4436" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SIP2</accession>
    <name>A0A2D0SIP2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Chemokine XC receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC108275738</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017342160.2">
        <property type="nucleotide sequence ID" value="XM_017486671.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SIP2"/>
    <dbReference type="GeneID" id="108275738"/>
    <dbReference type="KEGG" id="ipu:108275738"/>
    <dbReference type="OMA" id="RSHTKNR"/>
    <dbReference type="OrthoDB" id="10015690at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000130">
        <property type="entry name" value="Chemokine (C-C motif) receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000355">
        <property type="entry name" value="Chemokine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF730">
        <property type="entry name" value="CHEMOKINE XC RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00657">
        <property type="entry name" value="CCCHEMOKINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="31"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="69"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="47"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017342160.2"/>
        </source>
    </evidence>
    <sequence length="348" mass="39783" checksum="D9CF1289F2FB0C49" modified="2023-02-22" version="2">MDDNSQYDYAYVDNISDGMCNKEDVAKIGSIVVPLFLSVVVVLSLVGNILVLVILALYENLKSLTNIFILNLALSDLLFTFGLPFWACYYIWGWTLGDAACKTVNFFFYAGFYSSIVFLVLMTVQRYMAVVHPLSDWERGQGFALIPIVAWVMSIAVALPAPIYSSVVPDPANATQLYCEYNSTEGYFTVTYEQNWFFVCAFLIMGFCYVRILQTVFKTRTKKRHRTIRLIFCIVVTFFLGWAPYNLVIFLHTFTHHQIEFFTICEVTHHLDYAEYVSKLLAFSHCCLNPVFYVFVGVKFRNHLKEILQKILQTPLNKDMQQTRTGTIQSHGSLCSNGNNCRGHSTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SJB3</accession>
    <name>A0A2D0SJB3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein LOC108276035</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC108276035</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017342793.1">
        <property type="nucleotide sequence ID" value="XM_017487304.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SJB3"/>
    <dbReference type="GeneID" id="108276035"/>
    <dbReference type="KEGG" id="ipu:108276035"/>
    <dbReference type="OrthoDB" id="8746253at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46026:SF4">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46026">
        <property type="entry name" value="RHO-TYPE GUANINE NUCLEOTIDE EXCHANGE FACTOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="134"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017342793.1"/>
        </source>
    </evidence>
    <sequence length="402" mass="45843" checksum="4EE78659D4EC8FCD" modified="2017-12-20" version="1">MGCCSVTPITKGIDDVGPDEIELLEINGAGVWSLGESKLQVSLKNETDEPPKRSPPARNITKEGTDEPPHRSPPTKNLRNAMKEGSDPISAKESAQKRNSQVVLWGQTKDELHQKLYTHPIRDWEGRAAHTYGDIIYSSLVFLHNGYMRAMAERYLVLFSFHLLILALDNSAHDFIYEGILPLSAIDVRVISQHDPCAPHMFEISGPMVDSKIFICASAAEQRIWIENIKDRRCKSLRQQLSPSHSALSYLLPCDEGWKREELKRYLLRSPILQWEGSPIQHMGQPAYISLVHISNSQRQGSQERLIVLFPLDLLILSVDSQRLRVKYEGRLPRKSIRALERSARHGRLEFELTGDLMESLLVSCTYPEDYQSWIFQLQQPEKTQAFPNHTPPPLIPKKRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SLX1</accession>
    <name>A0A2D0SLX1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transferrin receptor protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22 23" type="primary">tfr2</name>
    </gene>
    <organism evidence="21 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22 23" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22 23">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Melanosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017343274.1">
        <property type="nucleotide sequence ID" value="XM_017487785.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017343275.1">
        <property type="nucleotide sequence ID" value="XM_017487786.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SLX1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005347"/>
    <dbReference type="GeneID" id="108276265"/>
    <dbReference type="KEGG" id="ipu:108276265"/>
    <dbReference type="CTD" id="7036"/>
    <dbReference type="OMA" id="QVVFNNH"/>
    <dbReference type="OrthoDB" id="5841748at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004998">
        <property type="term" value="F:transferrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140298">
        <property type="term" value="P:endocytic iron import into cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033572">
        <property type="term" value="P:transferrin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09848">
        <property type="entry name" value="M28_TfR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02128">
        <property type="entry name" value="PA_TfR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000101">
        <property type="entry name" value="N-acetylated alpha-linked acidic dipeptidase like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.40:FF:000002">
        <property type="entry name" value="Transferrin receptor protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000010">
        <property type="entry name" value="Transferrin receptor protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.40">
        <property type="entry name" value="Transferrin receptor-like, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007365">
        <property type="entry name" value="TFR-like_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036757">
        <property type="entry name" value="TFR-like_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037324">
        <property type="entry name" value="TfR1/2_PA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF33">
        <property type="entry name" value="TRANSFERRIN RECEPTOR PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04253">
        <property type="entry name" value="TFR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47672">
        <property type="entry name" value="Transferrin receptor-like dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0564">Palmitate</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14 22 23" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="18">
        <location>
            <begin position="227"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="20">
        <location>
            <begin position="404"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin receptor-like dimerisation" evidence="19">
        <location>
            <begin position="663"/>
            <end position="771"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017343274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017343275.1"/>
        </source>
    </evidence>
    <sequence length="782" mass="86020" checksum="E2CA46CB2783E61E" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SNU1</accession>
    <name>A0A2D0SNU1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Arf-GAP with dual PH domain-containing protein 1 isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC108276756</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017344161.1">
        <property type="nucleotide sequence ID" value="XM_017488672.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SNU1"/>
    <dbReference type="GeneID" id="108276756"/>
    <dbReference type="OrthoDB" id="73919at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902936">
        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005547">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13252">
        <property type="entry name" value="PH1_ADAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01251">
        <property type="entry name" value="PH2_ADAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000080">
        <property type="entry name" value="Arf-GAP with dual PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000099">
        <property type="entry name" value="Arf-GAP with dual PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052589">
        <property type="entry name" value="Arf-GAP_dual-PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037849">
        <property type="entry name" value="PH1_ADAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037851">
        <property type="entry name" value="PH2_ADAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46021:SF5">
        <property type="entry name" value="ARF-GAP WITH DUAL PH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46021">
        <property type="entry name" value="ARF-GAP WITH DUAL PH DOMAIN-CONTAINING PROTEIN 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="68"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="191"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017344161.1"/>
        </source>
    </evidence>
    <sequence length="313" mass="36712" checksum="0AFA4CE19F5EFA35" modified="2017-12-20" version="1">MGTARTAVLQLMASIGNSSAKAKYEQMVPAFYYSPTHTDCQLLREQWIRAKYERNEFMYVDRQEPYSAGYREGFLWKRGRDNGQFLSRKFILSERDGAMKYFNKHDAREPKALMKIQTINATFQPTKIGHPHGLQITYLKDNSTRNIFVYHEDGKEIVDWFNAIRAARFHYLQVAFPRAPDTELVPKLTRNYIKEGYMEKTGPKHTEGFKKRWFTLDDRRLMYFKDPLDAYARGEVFIGSKESGYTVVPGLPPSTQGYHWQFGITIVTPDRKFLFACETEEEQKEWIAVFQSVINRPMLPQEYAVEAHFKHKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SQC3</accession>
    <name>A0A2D0SQC3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Serine/threonine kinase 31 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">stk31</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
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    <dbReference type="OrthoDB" id="10023235at2759"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="term" value="P:P granule organization"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Ser_Thr_kinase"/>
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        <property type="entry name" value="SNase_OB-fold_sf"/>
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        <property type="entry name" value="Tudor"/>
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    <dbReference type="PANTHER" id="PTHR24361">
        <property type="entry name" value="MITOGEN-ACTIVATED KINASE KINASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24361:SF613">
        <property type="entry name" value="NUCLEAR RECEPTOR-BINDING PROTEIN-RELATED"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="S_TKc"/>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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        <location>
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    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="705"/>
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        </location>
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        <location>
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        <location>
            <begin position="216"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50304"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017344903.1"/>
        </source>
    </evidence>
    <sequence length="991" mass="112209" checksum="B415013E2B02F1CA" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SSJ9</accession>
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        <name evidence="7" type="primary">LOC108277345</name>
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    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
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        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
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    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="MFS_trans_sf"/>
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        <property type="entry name" value="THYMIC STROMAL COTRANSPORTER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZGC:174356"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter"/>
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    </dbReference>
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    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
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        <location>
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        </location>
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        </location>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017345476.1"/>
        </source>
    </evidence>
    <sequence length="420" mass="46303" checksum="041DB223DC80E4F6" modified="2017-12-20" version="1">MAFLRSHLEPVVFFAQIASSFFDTGLQMVVKQRYENTTIPGAGGEHRSIADFYMVFNILTKVVPIFPAFLLAQIGDKGYRKVPVVVPLIGYFVSRALLLLVIAFDWRVEVMYASPVVNGLCGGFSSYWPGVITLVSLSTSEEVRSLRIMRTELTYGLAGFLGSLASGHLFQLYALGHRQGFVLSCMSVVLYFVCLVYAAFILQVNTRTPSADERRDGESVGILNRDKVNIALLIAAGILYDVAVAGGVEILNVYVLVEPLSWTATQVGYGNAAGSVIFITSFLGVKLFTRCSLSDTSMIMIGMFSFLTGIYFMTFVTTTTTYYLARTLTLFALIPMPTIRSLLSKQIRGSSYGKTFTGLQLSFQFVGLATTYTYTEVYQSTLHILPGFVFTLSSIITFLAMIPISIIGCRTARQGCYERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0ST90</accession>
    <name>A0A2D0ST90_ICTPU</name>
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        <submittedName>
            <fullName evidence="6">Coiled-coil domain-containing protein 105</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ccdc105</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017345912.2">
        <property type="nucleotide sequence ID" value="XM_017490423.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0ST90"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000001496"/>
    <dbReference type="GeneID" id="108277611"/>
    <dbReference type="KEGG" id="ipu:108277611"/>
    <dbReference type="CTD" id="126402"/>
    <dbReference type="OrthoDB" id="9896158at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048256">
        <property type="entry name" value="Tektin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038949">
        <property type="entry name" value="TEKTL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35081">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35081:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03148">
        <property type="entry name" value="Tektin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="393"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017345912.2"/>
        </source>
    </evidence>
    <sequence length="452" mass="50400" checksum="FE51922D8CBD14E5" modified="2023-06-28" version="2">MKGVVRSAPCAAVRLGAQGRRDETLCSIRRAERLVLQYHVHAMDSATRYRTPPFPSKLPDLALLAHSVNGTQRDDDIKTRPKSPGTMLHSLGASSPFPPPALREKSAGLSVAVAAEYTRAVRQVEGRLRRQAGRITEEATRLDHQKEKLEKLLRSVRTALLINQKTTDERTRRPATETEKDGADHLLCHERKGLNELKQKLEILLRDTLTQLQILVQSSRQLLDCAFERSRVIDLLPQHGSPSAAVHLSLSPLSLKPDPSGPFTPECKEALESSAAVLQTSQQLSGSIEQVMCDAVIKQATLHRSVNEGLLKKIAETQSLQQRLTLSSAATRQAIYRKQRQMQCASYSHGRTLGPVSSDDLFCRERMNRPVVQAYERHPSVQLPESRLLTQGSVMLKQHLERAEKAVEELEVAHLQLEDDSCAKRVAASVDSAVVRLRRRNIPHMFVHSVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SYR2</accession>
    <name>A0A2D0SYR2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">GPI transamidase component PIG-S</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">pigs</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347807.1">
        <property type="nucleotide sequence ID" value="XM_017492318.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SYR2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012854"/>
    <dbReference type="GeneID" id="108278760"/>
    <dbReference type="KEGG" id="ipu:108278760"/>
    <dbReference type="CTD" id="94005"/>
    <dbReference type="OMA" id="AEHKYAV"/>
    <dbReference type="OrthoDB" id="28748at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019540">
        <property type="entry name" value="PtdIno-glycan_biosynth_class_S"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21072">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21072:SF13">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10510">
        <property type="entry name" value="PIG-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="527"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017347807.1"/>
        </source>
    </evidence>
    <sequence length="564" mass="62414" checksum="BE4777B5C025C8CE" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T659</accession>
    <name>A0A2D0T659_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Serine/threonine-protein kinase</fullName>
            <ecNumber evidence="12">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC108280133</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="12"/>
    <dbReference type="RefSeq" id="XP_017350387.1">
        <property type="nucleotide sequence ID" value="XM_017494898.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T659"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000015610"/>
    <dbReference type="GeneID" id="108280133"/>
    <dbReference type="KEGG" id="ipu:108280133"/>
    <dbReference type="OMA" id="WGTINGT"/>
    <dbReference type="OrthoDB" id="10252171at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14102">
        <property type="entry name" value="STKc_PIM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000209">
        <property type="entry name" value="Serine/threonine-protein kinase pim-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000232">
        <property type="entry name" value="Serine/threonine-protein kinase pim-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017348">
        <property type="entry name" value="PIM1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051138">
        <property type="entry name" value="PIM_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22984">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22984:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037993">
        <property type="entry name" value="STPK_Pim-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 12 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 12 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="41"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="47"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 15">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR037993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR037993-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR037993-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017350387.1"/>
        </source>
    </evidence>
    <sequence length="324" mass="36774" checksum="EA8F583FEDBB3528" modified="2017-12-20" version="1">MLLSKFGSFSHICSPNSVDHLPVKIQIQPVKVESEPFEKVYQVGSVLGSGGFGTVYAGSRISDGLPVAVKHVAKERVTEWGSINGVMVPLEIVLLKKVGSNFRGVIKLLDWYERPDGWLIVMERPELVKDLFDFITEKGALDEGTARGFFRQVLEAVRHCYSCGVVHRDIKDENLLVDLRTGELKLIDFGSGAILKDTVYTDFDGTRVYSPPEWIRFHRYHGRSATVWSLGVLLYDMVCGDIPFEQDEEILRGRLYFRRRISAECQQLIKWCLSLRPSDRPTLEQIFEHPWMRHAEASKTEEGDIRLCNISTDTSSMSSSKESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T9S3</accession>
    <name>A0A2D0T9S3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Serine/Arginine-related protein 53 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rsrc1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017351612.1">
        <property type="nucleotide sequence ID" value="XM_017496123.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T9S3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008725"/>
    <dbReference type="GeneID" id="108280754"/>
    <dbReference type="KEGG" id="ipu:108280754"/>
    <dbReference type="CTD" id="51319"/>
    <dbReference type="OMA" id="NYRQKYM"/>
    <dbReference type="OrthoDB" id="9946564at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000380">
        <property type="term" value="P:alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034604">
        <property type="entry name" value="SRRP53"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31968">
        <property type="entry name" value="SERINE/ARGININE-RELATED PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31968:SF4">
        <property type="entry name" value="SERINE_ARGININE-RELATED PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="203"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="25"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="79"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="93"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="131"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017351612.1"/>
        </source>
    </evidence>
    <sequence length="330" mass="38050" checksum="2EAAA3A2C35F8237" modified="2017-12-20" version="1">MGRRSSDSEDDSRSRRKKKHRRRSSSSSSSSSSSGSRVTSRSKHSSRRSHSRSRDRDRRRRHRSSSSSSRGSSHRKRSRSTERRRSRSADRGRSHRSRRSRSRSRRRSRSRHRRSRSRSSGRSSRRRSRSRDRDRSQRKTRDKDRDREKDRGRDRGRDKEDAKAKEKGKQGDAVNIKAGLEHLTAAEQAKVRLQLVLQAAAKTDEALRAKEKKEEEAKKIKEEEEVSLAEQVRRIKEIEAIESDSFVPQAFKSSRDAAKVTESLEIQEAGSGVTPTVKEEDIALPTVIKYSNSDSLAHPSLFIDKEEAEKLWLNRLLSLRQERLMGSPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0TAG0</accession>
    <name>A0A2D0TAG0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16 17">Polycomb complex protein BMI-1-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16 17" type="primary">bmi1b</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16 17" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16 17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of rnf2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of a PRC1-like complex. Homodimer. Interacts with cbx2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017351863.1">
        <property type="nucleotide sequence ID" value="XM_017496374.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017351865.1">
        <property type="nucleotide sequence ID" value="XM_017496376.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0TAG0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000004454"/>
    <dbReference type="GeneID" id="108280883"/>
    <dbReference type="KEGG" id="ipu:108280883"/>
    <dbReference type="CTD" id="394249"/>
    <dbReference type="GeneTree" id="ENSGT00940000156042"/>
    <dbReference type="OMA" id="MKMHRTT"/>
    <dbReference type="OrthoDB" id="1305878at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035102">
        <property type="term" value="C:PRC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021549">
        <property type="term" value="P:cerebellum development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17165">
        <property type="entry name" value="RAWUL_PCGF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16736">
        <property type="entry name" value="RING-HC_PCGF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000106">
        <property type="entry name" value="Polycomb complex protein BMI-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000082">
        <property type="entry name" value="Polycomb group ring finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032443">
        <property type="entry name" value="RAWUL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10825:SF21">
        <property type="entry name" value="POLYCOMB COMPLEX PROTEIN BMI-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10825">
        <property type="entry name" value="RING FINGER DOMAIN-CONTAINING, POLYCOMB GROUP COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16207">
        <property type="entry name" value="RAWUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="20"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="239"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="261"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00062186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017351863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017351865.1"/>
        </source>
    </evidence>
    <sequence length="328" mass="36657" checksum="F9BB7867BFBDB76C" modified="2017-12-20" version="1">MTMHRTTRIKITELNPHLMCVLCGGYFIDATTIVECLHSFCKMCIVRYLETSKYCPICDVQVHKTKPLLNIRSDKTLQDIVYKLVPGLFKNEMKRRRDFYAAHPSVDAANGSNEDRGEVADEDKRIIADDEIISLSIEFYDQNRLGSVDGEDKEPTKETNVKRYLQCPAAMTVMHLRKFLRSKMDIPCTFQIEVMYEDEPLKDYYTLMDIAYIYTWRRNGPLPLKYRVRPSCKKLKLSSAPSCETAGRKPETESDSGSEKLSSPVLRTPTPSTSSSLPSQSAPVKSSQSSFSHLAPVNGVSNKAGPNGQVSFSGKASKGSVNGSNSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979E1D0</accession>
    <name>A0A979E1D0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ADAMTS-like protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">adamtsl2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_047005767.1">
        <property type="nucleotide sequence ID" value="XM_047149811.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979E1D0"/>
    <dbReference type="GeneID" id="108256019"/>
    <dbReference type="CTD" id="9719"/>
    <dbReference type="OrthoDB" id="5781878at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.830:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000005">
        <property type="entry name" value="ADAM metallopeptidase with thrombospondin type 1 motif 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013273">
        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010909">
        <property type="entry name" value="PLAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF147">
        <property type="entry name" value="ADAMTS-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08686">
        <property type="entry name" value="PLAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50900">
        <property type="entry name" value="PLAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5037217578" evidence="8">
        <location>
            <begin position="26"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="domain" description="PLAC" evidence="9">
        <location>
            <begin position="911"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="375"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="378"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="69"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="73"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="84"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_047005767.1"/>
        </source>
    </evidence>
    <sequence length="951" mass="106324" checksum="07E13C2C272D7818" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979E5V5</accession>
    <name>A0A979E5V5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">E3 SUMO-protein ligase RanBP2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">Ran-binding protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="34" type="primary">ranbp2</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="33">SDA103</strain>
        </source>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="34">Blood</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="23">Belongs to the RanBP2 E3 ligase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047009618.2">
        <property type="nucleotide sequence ID" value="XM_047153662.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108261082"/>
    <dbReference type="CTD" id="5903"/>
    <dbReference type="OrthoDB" id="2357150at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019789">
        <property type="term" value="F:SUMO transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051168">
        <property type="term" value="P:nuclear export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="E3 SUMO-protein ligase RanBP2"/>
        <property type="match status" value="4"/>
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        <property type="entry name" value="E3 SUMO-protein ligase RanBP2"/>
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    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022011">
        <property type="entry name" value="IR1-M"/>
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    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
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    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
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        <property type="entry name" value="TPR-like_helical_dom_sf"/>
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        <property type="entry name" value="TPR_rpt"/>
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        <property type="entry name" value="Znf_RanBP2"/>
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        <property type="entry name" value="Znf_RanBP2_sf"/>
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        <property type="entry name" value="RAN BINDING PROTEIN"/>
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        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="4"/>
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        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="5"/>
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        <property type="entry name" value="CSAPPISMRASE"/>
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        <property type="entry name" value="RanBD"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Ran binding protein zinc finger-like"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="5"/>
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        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="5"/>
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    <keyword evidence="28" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="21" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="13" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="19" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="22" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0653">Protein transport</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="16" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="27" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="19" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 26" id="KW-0863">Zinc-finger</keyword>
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            <begin position="60"/>
            <end position="93"/>
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            <begin position="1149"/>
            <end position="1285"/>
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    </feature>
    <feature type="domain" description="RanBP2-type" evidence="32">
        <location>
            <begin position="1411"/>
            <end position="1440"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="32">
        <location>
            <begin position="1488"/>
            <end position="1517"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="32">
        <location>
            <begin position="1619"/>
            <end position="1648"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="32">
        <location>
            <begin position="1670"/>
            <end position="1699"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="32">
        <location>
            <begin position="1702"/>
            <end position="1731"/>
        </location>
    </feature>
    <feature type="domain" description="RanBD1" evidence="31">
        <location>
            <begin position="1887"/>
            <end position="2023"/>
        </location>
    </feature>
    <feature type="domain" description="RanBD1" evidence="31">
        <location>
            <begin position="2181"/>
            <end position="2317"/>
        </location>
    </feature>
    <feature type="domain" description="RanBD1" evidence="31">
        <location>
            <begin position="2729"/>
            <end position="2864"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="30">
        <location>
            <begin position="2885"/>
            <end position="3041"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="958"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="1056"/>
            <end position="1081"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="1118"/>
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    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="1283"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="1352"/>
            <end position="1390"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="1437"/>
            <end position="1464"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="1513"/>
            <end position="1543"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="1576"/>
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    <feature type="region of interest" description="Disordered" evidence="29">
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            <begin position="1734"/>
            <end position="1754"/>
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            <begin position="2150"/>
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            <begin position="2349"/>
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            <begin position="2397"/>
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        <location>
            <begin position="2588"/>
            <end position="2661"/>
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            <begin position="805"/>
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        <location>
            <begin position="1000"/>
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            <begin position="1123"/>
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            <begin position="2414"/>
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            <begin position="2607"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="2624"/>
            <end position="2635"/>
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    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004126"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00004718"/>
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            <dbReference type="ARBA" id="ARBA00022448"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    <evidence type="ECO:0000256" key="11">
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            <dbReference type="ARBA" id="ARBA00022771"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00070141"/>
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    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00081161"/>
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    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
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    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
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    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
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    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
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    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="RefSeq" id="XP_047009618.2"/>
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    <sequence length="3042" mass="339209" checksum="3826C8FF60F9687E" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QUP1</accession>
    <name>A0A9F7QUP1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC108255390 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108255390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">SDA103</strain>
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="3">Blood</tissue>
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<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Ictalurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="20">SDA103</strain>
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    <reference evidence="21" key="2">
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        <scope>IDENTIFICATION</scope>
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    <dbReference type="InterPro" id="IPR036770">
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    <dbReference type="InterPro" id="IPR040745">
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        <property type="entry name" value="Multifunctional_ANK_Repeat"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24123:SF49">
        <property type="entry name" value="ANKYRIN-2-LIKE ISOFORM X1"/>
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            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">SDA103</strain>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23005:SF4">
        <property type="entry name" value="OXYGEN-REGULATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23005">
        <property type="entry name" value="RETINITIS PIGMENTOSA 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_053537455.1"/>
        </source>
    </evidence>
    <sequence length="433" mass="48766" checksum="B4C8B267151C3B43" modified="2023-06-28" version="1">MTSSTKSRPFSPSSERYIVNQLHNSFAESAYDPTGSVEMETGQLLESVAETDTVTCIDGDPNECISMPTDDDVEKSFRVNQDGSMTVEMRVRLTIKEEETVHWTTTLSRQSVTSVMKSDLDSHSDLGSALANKIAPDVGSDGSAIQRCPEEYSNVSTNKCLKALYEEMTEGRTEESITVKDPLQPLPEQKQISVETKSQDTVQRLDTSREEASNEQEHCLVRQCNKHPVPKPRSKGTSQLQASLYKSAENLQLQDSGQETVLHIYEQQTRQKGFLANTQFYDQGICTCGTVFFAPLDMVPTSSKDLGTEPRPTTVYGSLQRQETFFPSLELQKSCEQNTSPEIKNKMSKMHTPLTEYSKTSAPRMIPTADTSKKRKKVRVIMKKSHIFHMATHEYWRKDNKIDILKEIKKIRAAIFNQAGVMREFSDANSKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R618</accession>
    <name>A0A9F7R618_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">AN1-type zinc finger protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">zfand1</name>
        <name evidence="7" type="synonym">zfan1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053530651.1">
        <property type="nucleotide sequence ID" value="XM_053674676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R618"/>
    <dbReference type="GeneID" id="100528619"/>
    <dbReference type="CTD" id="79752"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035617">
        <property type="term" value="P:stress granule disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057358">
        <property type="entry name" value="UBL_ZFAND1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14677:SF37">
        <property type="entry name" value="AN1-TYPE ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14677">
        <property type="entry name" value="ARSENITE INDUCUBLE RNA ASSOCIATED PROTEIN AIP-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25327">
        <property type="entry name" value="UBL_ZFAND1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="AN1-type" evidence="5">
        <location>
            <begin position="13"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053530651.1"/>
        </source>
    </evidence>
    <sequence length="225" mass="24884" checksum="49BFBFF611FF0B42" modified="2023-06-28" version="1">MVSRKRDVLDSGVITSYPCTLQDCKGRELLPVICQHCQKHFCFVHRHQEDHKCEKLETPQPRMVATQELVQKIVESKKNAPTSKGRKGAKNSATAAKVALMKLKLHATGDKGLPQTERTYFQVFLPKDLNTSSLPMFFSSKWTVGKVVDFAAAQASLKNNNNVLTAKKLRLCHPETGEAIKMDACLQALLSLSDSPLYNGGNVILEYLDNESSGVEDVTAYISSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R7F4</accession>
    <name>A0A9F7R7F4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Synaptonemal complex central element protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">si:ch211-199g17.9</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SYCE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053531401.1">
        <property type="nucleotide sequence ID" value="XM_053675426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R7F4"/>
    <dbReference type="GeneID" id="108256969"/>
    <dbReference type="KEGG" id="ipu:108256969"/>
    <dbReference type="OrthoDB" id="8931744at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000795">
        <property type="term" value="C:synaptonemal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007130">
        <property type="term" value="P:synaptonemal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026676">
        <property type="entry name" value="SYCE1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21731">
        <property type="entry name" value="SYNAPTONEMAL COMPLEX CENTRAL ELEMENT PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21731:SF1">
        <property type="entry name" value="SYNAPTONEMAL COMPLEX CENTRAL ELEMENT PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15233">
        <property type="entry name" value="SYCE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0469">Meiosis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="28"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="183"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053531401.1"/>
        </source>
    </evidence>
    <sequence length="205" mass="23926" checksum="FE30D17D80009E68" modified="2023-06-28" version="1">MSVVESSFNVEDVFRFPQAPGREKDLKIEELLSKLRKLQQVKLVLEEEVKEALSLCSTLRDEDNALTAEILQLQGILSEKEETCRSLQFKLEDLEQESQKQSELKQQKEELVQQYSCQIQETKLRHRKIRMKFENQLQQLIGQHKNFCTLFTPERIPTEIQSAEYATEQLLKAEKQKVEQVENLQEELSNAQSTNMPQDAKTAAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R9I4</accession>
    <name>A0A9F7R9I4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Rhamnose-binding lectin-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC108264892</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053535972.1">
        <property type="nucleotide sequence ID" value="XM_053679997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R9I4"/>
    <dbReference type="GeneID" id="108264892"/>
    <dbReference type="OrthoDB" id="1100386at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22836">
        <property type="entry name" value="Gal_Rha_Lectin_RBL_rpt2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.740:FF:000001">
        <property type="entry name" value="Adhesion G protein-coupled receptor L2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.740:FF:000003">
        <property type="entry name" value="Protein eva-1 homolog C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.740">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000922">
        <property type="entry name" value="Lectin_gal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043159">
        <property type="entry name" value="Lectin_gal-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46780">
        <property type="entry name" value="PROTEIN EVA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02140">
        <property type="entry name" value="SUEL_Lectin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50228">
        <property type="entry name" value="SUEL_LECTIN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0348">Hemagglutinin</keyword>
    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039938622" evidence="4">
        <location>
            <begin position="24"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="5">
        <location>
            <begin position="123"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="5">
        <location>
            <begin position="218"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="5">
        <location>
            <begin position="384"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="5">
        <location>
            <begin position="479"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053535972.1"/>
        </source>
    </evidence>
    <sequence length="572" mass="62431" checksum="BD55E80D1BDB7D6A" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RD19</accession>
    <name>A0A9F7RD19_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Elongation factor Tu</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">tufm</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with NLRX1. Interacts with ATG16L1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053539931.1">
        <property type="nucleotide sequence ID" value="XM_053683956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RD19"/>
    <dbReference type="GeneID" id="108272996"/>
    <dbReference type="KEGG" id="ipu:108272996"/>
    <dbReference type="CTD" id="7284"/>
    <dbReference type="OMA" id="EGDKEWG"/>
    <dbReference type="OrthoDB" id="2067at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070125">
        <property type="term" value="P:mitochondrial translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01884">
        <property type="entry name" value="EF_Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03697">
        <property type="entry name" value="EFTU_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03706">
        <property type="entry name" value="mtEFTU_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000068">
        <property type="entry name" value="Elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000071">
        <property type="entry name" value="Elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000003">
        <property type="entry name" value="Elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_B">
        <property type="entry name" value="EF_Tu_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041709">
        <property type="entry name" value="EF-Tu_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033720">
        <property type="entry name" value="EFTU_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004541">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_bac/org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00485">
        <property type="entry name" value="EF-Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000766">
        <property type="entry name" value="PRK00049.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009372">
        <property type="entry name" value="PRK12735.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009373">
        <property type="entry name" value="PRK12736.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF36">
        <property type="entry name" value="ELONGATION FACTOR TU, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 16 19" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="13 16" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tr-type G" evidence="17">
        <location>
            <begin position="50"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00065637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_053539931.1"/>
        </source>
    </evidence>
    <sequence length="446" mass="49082" checksum="6394347C7810B189" modified="2023-06-28" version="1">MAALVGIRACLSALQLSSPSLLHSSYKLCAVPLSRRNFAAEAKRVFSRDKPHVNIGTIGHVDHGKTTLTAAITKVLAEAGGARYKKYEDIDNAPEEKARGITINASHVEYTTANRHYAHTDCPGHADYVKNMITGTAQLDGCILVVAATDGQMPQTREHLLLARQIGVQHVVVYVNKADAVDDPELLQLVELEIRELLSEFGYDGDNTPVICGSALCALENRQPELGVNSIMKLLEVVDEYIPLPRRDLDRPFLLPIEGVYSIPGRGTVVTGTLERGVIKKGDECEFVGHNRSAKSVVTGIEMFHQSLDRAEAGDNLGALVRGMKREDMRRGVVMCKPGSVQPHQKIKAQVYILSKEEGGRHKPFVTNFMPVMFSLTWDMACRIQLPEGKEMVMPGEDTSLTLTLRQPMVLEKGQRFTLRDGNKTIGTGLVTDILPTTPEDQHNWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RF83</accession>
    <name>A0A9F7RF83_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Paired box protein Pax-2b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">pax2b</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053541086.1">
        <property type="nucleotide sequence ID" value="XM_053685111.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RF83"/>
    <dbReference type="GeneID" id="108273591"/>
    <dbReference type="KEGG" id="ipu:108273591"/>
    <dbReference type="CTD" id="60638"/>
    <dbReference type="OrthoDB" id="3225452at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048593">
        <property type="term" value="P:camera-type eye morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030902">
        <property type="term" value="P:hindbrain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00131">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000013">
        <property type="entry name" value="Paired box 8 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000003">
        <property type="entry name" value="Paired box protein Pax-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043182">
        <property type="entry name" value="PAIRED_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001523">
        <property type="entry name" value="Paired_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022130">
        <property type="entry name" value="Pax2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043565">
        <property type="entry name" value="PAX_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636:SF19">
        <property type="entry name" value="PAIRED BOX PROTEIN PAX-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636">
        <property type="entry name" value="PAIRED BOX PROTEIN PAX-6-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00292">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12403">
        <property type="entry name" value="Pax2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00027">
        <property type="entry name" value="PAIREDBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00351">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00034">
        <property type="entry name" value="PAIRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51057">
        <property type="entry name" value="PAIRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0563">Paired box</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Paired" evidence="9">
        <location>
            <begin position="16"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="144"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="277"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="144"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="209"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="281"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053541086.1"/>
        </source>
    </evidence>
    <sequence length="387" mass="41600" checksum="1D92A9ACB81DCE5F" modified="2023-06-28" version="1">MDIHCKADPFSAMHPGHGGVNQLGGVFVNGRPLPDVVRQRIVELAHQGVRPCDISRQLRVSHGCVSKILGRYYETGSIKPGVIGGSKPKVATPKVVDKIADYKRHNPTMFAWEIRDRLLAEGICDNDTVPSVSSINRIIRTKVQQQYHPSPDGTSLSTPGHTIVPSTASPPVSSGSSDPVGSYSINGILGIPRSNGEKRKRDDDGSEGSGQNSDSQGSVESLRKHLRADAFTQQQLEALDRVFERPAYPDVFHNTEHIKPEQASEYALPALNPGLDEVKPSLSSSSSSDLGASVSQSYPVGRDMANTTLPGYPPHVPPTGQGSYPTSTLAGMVPGSEFSGNPYSHPQYTAYNEAWRFSNPALLSSPYYYSASRASAPPAAAAAYDRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RGF4</accession>
    <name>A0A9F7RGF4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein fem-1 homolog B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">FEM1-beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">fem1b</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the fem-1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053541716.1">
        <property type="nucleotide sequence ID" value="XM_053685741.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RGF4"/>
    <dbReference type="GeneID" id="108275318"/>
    <dbReference type="KEGG" id="ipu:108275318"/>
    <dbReference type="CTD" id="10116"/>
    <dbReference type="OMA" id="ISTKTTC"/>
    <dbReference type="OrthoDB" id="4429489at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000205">
        <property type="entry name" value="Fem-1 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173">
        <property type="entry name" value="ANKYRIN REPEAT CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173:SF78">
        <property type="entry name" value="PROTEIN FEM-1 HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="87"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="120"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="153"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="186"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="531"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00072197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00076407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053541716.1"/>
        </source>
    </evidence>
    <sequence length="627" mass="70377" checksum="C7590B86A9F68033" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RH22</accession>
    <name>A0A9F7RH22_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Vigilin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC128634071</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053540022.1">
        <property type="nucleotide sequence ID" value="XM_053684047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RH22"/>
    <dbReference type="GeneID" id="128634071"/>
    <dbReference type="KEGG" id="ipu:128634071"/>
    <dbReference type="OrthoDB" id="6280827at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22417">
        <property type="entry name" value="KH-I_Vigilin_rpt14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22418">
        <property type="entry name" value="KH-I_Vigilin_rpt15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="31"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="104"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="217"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="217"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053540022.1"/>
        </source>
    </evidence>
    <sequence length="267" mass="29581" checksum="84E8046DA17687BE" modified="2023-06-28" version="1">METPCHSPGPKEGLLECIKGLQAELEDRALRSFKLTITVDPKYHLKIIARKGAIITQIHTDHDVNIQFPEKNDENHDQLSMTGYEQNTIAARDAIQGIVVELEEIISEDITLDSRVHARIIGARTKGIRKIMDEFKVDLRFPQSGAADPNLVTVTGRPEMVDEAIDHLLNLEEEYMADVVEDEAKMAYMKPSGTAAAKPEEPRGPSNGFVVREAPWATSSEKASDMNSSEDFPSFGTPVAAAKASPWGPERFRSDLWLVDVSVSSMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RQ04</accession>
    <name>A0A9F7RQ04_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">LOW QUALITY PROTEIN: zinc finger protein 665</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC108272479</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053539967.1">
        <property type="nucleotide sequence ID" value="XM_053683992.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108272479"/>
    <dbReference type="KEGG" id="ipu:108272479"/>
    <dbReference type="OrthoDB" id="8720956at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045596">
        <property type="term" value="P:negative regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19193">
        <property type="entry name" value="PR-SET_PRDM7_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001155">
        <property type="entry name" value="Zinc finger 30C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000512">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002005">
        <property type="entry name" value="Zinc finger protein 200"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050589">
        <property type="entry name" value="Ikaros_C2H2-ZF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044417">
        <property type="entry name" value="PRDM7_9_PR-SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404:SF114">
        <property type="entry name" value="KLUMPFUSS, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21549">
        <property type="entry name" value="PRDM2_PR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="22"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="14">
        <location>
            <begin position="182"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="362"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="431"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="459"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="487"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="515"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="543"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="571"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="599"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="627"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="655"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="683"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="711"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="739"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="767"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="795"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="823"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="851"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="879"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="907"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="935"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="963"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="991"/>
            <end position="1018"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_053539967.1"/>
        </source>
    </evidence>
    <sequence length="1030" mass="118696" checksum="AD7E72B32A2309A0" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TF24</accession>
    <name>A0A9F7TF24_ICTPU</name>
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        <submittedName>
            <fullName evidence="5 6 7">Uncharacterized protein LOC128628723 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6 7" type="primary">LOC128628723</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5 6 7" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5 6 7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053530806.1">
        <property type="nucleotide sequence ID" value="XM_053674831.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053530807.1">
        <property type="nucleotide sequence ID" value="XM_053674832.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053530808.1">
        <property type="nucleotide sequence ID" value="XM_053674833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TF24"/>
    <dbReference type="GeneID" id="128628723"/>
    <dbReference type="KEGG" id="ipu:128628723"/>
    <dbReference type="OrthoDB" id="8887244at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46013:SF4">
        <property type="entry name" value="B-CELL RECEPTOR CD22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46013">
        <property type="entry name" value="VASCULAR CELL ADHESION MOLECULE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="72"/>
            <end position="160"/>
        </location>
    </feature>
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        <location>
            <begin position="204"/>
            <end position="243"/>
        </location>
    </feature>
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        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053530806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_053530807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053530808.1"/>
        </source>
    </evidence>
    <sequence length="285" mass="31699" checksum="DC8134389286A91B" modified="2023-06-28" version="1">MLWCSMNSNTGVCTDPPYVYNSSSNTTSDFEYAGDDKSDCTLLIHNVQFSYSGEYRFRFITVQTGGRWTGVPGATLQVADLKVSLIRLSGIGTLKQGDSLNLTCDVNCTQSSSQFVWSKNNERLNTSGPVLHFPAVTVRDSGSYTCTWKTNRTSGSETISLLVEGENPERDFAQWHIWIIVLVIVAVILVIVAAVINHGRKRVEAEEENSGKGAEKAQQVGENTHDKPTKPQINNVPQLDDETLHTEEVTYATVFVKDKKLEQGRVHTERQEEDDSVIYSTVKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TFG9</accession>
    <name>A0A9F7TFG9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DOMON domain-containing protein FRRS1L</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC108256422</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053530956.1">
        <property type="nucleotide sequence ID" value="XM_053674981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TFG9"/>
    <dbReference type="GeneID" id="108256422"/>
    <dbReference type="KEGG" id="ipu:108256422"/>
    <dbReference type="OrthoDB" id="6418377at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900449">
        <property type="term" value="P:regulation of glutamate receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099072">
        <property type="term" value="P:regulation of postsynaptic membrane neurotransmitter receptor levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09628">
        <property type="entry name" value="DOMON_SDR_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005018">
        <property type="entry name" value="DOMON_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042789">
        <property type="entry name" value="FRRS1L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46902">
        <property type="entry name" value="DOMON DOMAIN-CONTAINING PROTEIN FRRS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46902:SF1">
        <property type="entry name" value="DOMON DOMAIN-CONTAINING PROTEIN FRRS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03351">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00664">
        <property type="entry name" value="DoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50836">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039932002" evidence="3">
        <location>
            <begin position="22"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="258"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="DOMON" evidence="4">
        <location>
            <begin position="105"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_053530956.1"/>
        </source>
    </evidence>
    <sequence length="279" mass="31659" checksum="890FA2415BF29D65" modified="2023-06-28" version="1">MFLVQLSLLLLEPSLWRLITGSPTDDNTLRGGHGEQGEPGHTEHHKDSYSTFASEFLESRYLSDEGYPFPTAPPVDPFARIKVSDCGVTKGCIRYGKPGCDAETCEYFLSYRRIGTDVEFEMSADTDGWVAVGFSSDKKMGGDDVMGCVHDDNGRVRIHHFYNVGQWAKEIRRNPARDEEGLFESNRVTCRFKRPLHVPREETLVDLHLSWYYLFAWGPAIQGSITRHDIDTPPVSDRMISIYKYEDIFMPATAYQTFSSPFCLLLIVALTFYLLMGSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TL38</accession>
    <name>A0A9F7TL38_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein si:dkey-21e13.3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">si:dkey-21e13.3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
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                <person name="Liu S."/>
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                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
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                <person name="Gao S."/>
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                <person name="Koren S."/>
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                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
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            <strain evidence="11">SDA103</strain>
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        <location>
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        </source>
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    </evidence>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053542146.1">
        <property type="nucleotide sequence ID" value="XM_053686171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TR28"/>
    <dbReference type="GeneID" id="108276136"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000015">
        <property type="entry name" value="Protein-L-isoaspartate (D-aspartate) O-methyltransferase domain-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579:SF2">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01135">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053542146.1"/>
        </source>
    </evidence>
    <sequence length="246" mass="27641" checksum="FE32BC25BA7DC404" modified="2023-06-28" version="1">MGGAVSAGEDNDELIDNLKEAHYIRSELVERAFRAIDRADYYLEEYKDSAYKDLAWRHGNLHLSAPCIYSEVMEALELQPGLSFLNLGSGTGYLSTMVGLILGPFGVNHGVELHVDVVEYAYQKLDYFIKTSDSFDRFEFCEPSFVVGNCLEILPESRQYDRVYCGAGVQKEYENYMKNLLKVGGILVLPLEEKLTKITRSGQNSWETKKIIAVTFAPLVQPKQSINGRGKSVPLQIPAPLCSEQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3TDQ3</accession>
    <name>E3TDQ3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">glycogenin glucosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.186</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">GLYG</name>
        <name evidence="19" type="synonym">glyg</name>
        <name evidence="19" type="synonym">gyg1b</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 19" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Glycogenin participates in the glycogen biosynthetic process along with glycogen synthase and glycogen branching enzyme. It catalyzes the formation of a short alpha (1,4)-glucosyl chain covalently attached via a glucose 1-O-tyrosyl linkage to internal tyrosine residues and these chains act as primers for the elongation reaction catalyzed by glycogen synthase.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Self-glucosylating initiator of glycogen synthesis. It catalyzes the formation of a short alpha (1,4)-glucosyl chain covalently attached via a glucose 1-O-tyrosyl linkage to internal tyrosine residues and these chains act as primers for the elongation reaction catalyzed by glycogen synthase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-tyrosyl-[glycogenin] + UDP-alpha-D-glucose = alpha-D-glucosyl-L-tyrosyl-[glycogenin] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23360"/>
            <dbReference type="Rhea" id="RHEA-COMP:14604"/>
            <dbReference type="Rhea" id="RHEA-COMP:14605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:140573"/>
            <dbReference type="EC" id="2.4.1.186"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:23361"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>[1,4-alpha-D-glucosyl](n)-L-tyrosyl-[glycogenin] + UDP-alpha-D-glucose = [1,4-alpha-D-glucosyl](n+1)-L-tyrosyl-[glycogenin] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56560"/>
            <dbReference type="Rhea" id="RHEA-COMP:14606"/>
            <dbReference type="Rhea" id="RHEA-COMP:14607"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:140574"/>
            <dbReference type="EC" id="2.4.1.186"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:56561"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyltransferase 8 family. Glycogenin subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.186" evidence="11"/>
    <dbReference type="EMBL" id="GU588483">
        <property type="protein sequence ID" value="ADO28439.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001188099.1">
        <property type="nucleotide sequence ID" value="NM_001201170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TDQ3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000001356"/>
    <dbReference type="CAZy" id="GT8">
        <property type="family name" value="Glycosyltransferase Family 8"/>
    </dbReference>
    <dbReference type="GeneID" id="100528900"/>
    <dbReference type="KEGG" id="ipu:100528900"/>
    <dbReference type="CTD" id="415152"/>
    <dbReference type="OrthoDB" id="2014201at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008466">
        <property type="term" value="F:glycogenin glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02537">
        <property type="entry name" value="GT8_Glycogenin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000092">
        <property type="entry name" value="Glycogenin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050587">
        <property type="entry name" value="GNT1/Glycosyltrans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11183">
        <property type="entry name" value="GLYCOGENIN SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ADO28439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="NP_001188099.1"/>
        </source>
    </evidence>
    <sequence length="323" mass="36754" checksum="CCB0AFA096A711FF" modified="2011-01-11" version="1">MADQAFVTLATNDKYAKGAMVLGKSLRNHKTSKKLVVLIGRHVSEPSRAVLQTLYDEVRLVDVLDSGDTAHLVMMQRPDLGVTFTKLHCWTLTHYSKCVFMDADTMVVANIDELFDREEFSAAPDPGWPDCFNSGVFVFRPSNETYGKLLEYCTEHGSFDGGDQGVLNGYFSDWATADIRKHLPFIYNMSSIAIYTYLPAFKQYGANAKVVHFLGKTKPWSYTYDTNQRRVWGNVQEASTHPGYLLEWWSLYSSCVLPLKHQEHGEEPFEFSVQQEEFQPQALPGPPPLSSAERKQRWEQGQADYMGMDSFENIEKKLAAFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3TER9</accession>
    <name>E3TER9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytochrome b-c1 complex subunit 10</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="1">Ubiquinol-cytochrome c reductase 6.4 kDa subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108280490</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="GU588851">
        <property type="protein sequence ID" value="ADO28805.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_017495513.3"/>
    </dbReference>
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    <dbReference type="STRING" id="7998.ENSIPUP00000028645"/>
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    <dbReference type="GeneTree" id="ENSGT01000000214836"/>
    <dbReference type="OMA" id="QHYWELA"/>
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        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Single_a-helix_sf"/>
    </dbReference>
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        <property type="entry name" value="UQCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15420:SF2">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15420">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 6.4 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08997">
        <property type="entry name" value="UCR_6-4kD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81518">
        <property type="entry name" value="Subunit XI (6.4 kDa protein) of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADO28805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017351002.1"/>
        </source>
    </evidence>
    <sequence length="55" mass="6107" checksum="E754670D3D1ACFF9" modified="2011-01-11" version="1">MLAKLIGQKYLSIGKAWVPTLAAWGGVGGIAVIHFTDWRLILDYVPYINGKFKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3TEY7</accession>
    <name>E3TEY7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ankyrin repeat domain-containing protein 16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 13" type="primary">ANR16</name>
        <name evidence="13" type="synonym">ankrd16</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Required to prevent the misactivation of serine (Ser) with tRNA(Ala) by promoting the hydrolysis of Ser-mischarged tRNA(Ala), thereby playing a role in translational fidelity. Binds directly to the catalytic domain of AARS/AlaRS and captures Ser that is misactivated by AARS/AlaRS, preventing the charging of Ser adenylates to tRNA(Ala) and precluding Ser misincorporation in nascent peptides.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with AARS; the interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GU588920">
        <property type="protein sequence ID" value="ADO28873.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001316232.1">
        <property type="nucleotide sequence ID" value="NM_001329303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TEY7"/>
    <dbReference type="GeneID" id="108279820"/>
    <dbReference type="KEGG" id="ipu:108279820"/>
    <dbReference type="CTD" id="54522"/>
    <dbReference type="OrthoDB" id="4772757at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000336">
        <property type="entry name" value="Ankyrin repeat domain-containing protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050889">
        <property type="entry name" value="Dendritic_Spine_Reg/Scaffold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24166:SF48">
        <property type="entry name" value="PROTEIN VAPYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24166">
        <property type="entry name" value="ROLLING PEBBLES, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 10" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="77"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="110"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="280"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADO28873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="NP_001316232.1"/>
        </source>
    </evidence>
    <sequence length="349" mass="39055" checksum="E04A0370F35E24E4" modified="2011-01-11" version="1">MSDENIYKLLIKLTQDGHLCKIKSQIEETSEIKQSMNAHVGKSGDTLLHYAARHGHLDIVKYFVEDLNVDVELHNNDYKRALHEASSMCHEQCVRYLLDKGAKIDCLKKADWTPLMMACTRRNLEVIRVLLDHGADPTLQNKDGWNSFHIACREGDPAVIEHLLHIRPEVWRTESKTRRTPLHTAAMHGCLEVVKILLERCDYKPDEKDSCGVTPFMDAVRNGHLGVAKLLLDKHQACPTAVDILGAQPLHQASVTAQEEALRFLVHELSVDVNVRATALELTALHYAAKEGHTDTIKTLLQLGADLHARDKKGRSALHMACIGQHAITARALLQLGLTDTEGQHGHYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2003-06-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q804T0</accession>
    <name>Q804T0_ICTPU</name>
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        <recommendedName>
            <fullName evidence="13">Protein-tyrosine sulfotransferase</fullName>
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        </recommendedName>
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    <gene>
        <name evidence="18" type="primary">tpst2</name>
        <name evidence="16" type="synonym">TPST2</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 18" key="1">
        <citation type="journal article" date="2003" name="J. Biol. Chem." volume="278" first="24243" last="24246">
            <title>The biology and enzymology of protein tyrosine O-sulfation.</title>
            <authorList>
                <person name="Moore K.L."/>
            </authorList>
            <dbReference type="PubMed" id="12730193"/>
            <dbReference type="DOI" id="10.1074/jbc.R300008200"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Head</tissue>
        </source>
    </reference>
    <reference evidence="16 18" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 13">Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>L-tyrosyl-[protein] + 3'-phosphoadenylyl sulfate = O-sulfo-L-tyrosine-[protein] + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16801"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:11688"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:65286"/>
            <dbReference type="EC" id="2.8.2.20"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the protein sulfotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.20" evidence="13"/>
    <dbReference type="EMBL" id="AF510735">
        <property type="protein sequence ID" value="AAO42985.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JT418716">
        <property type="protein sequence ID" value="AHH42822.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187093.1">
        <property type="nucleotide sequence ID" value="NM_001200164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q804T0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019627"/>
    <dbReference type="GeneID" id="100304582"/>
    <dbReference type="KEGG" id="ipu:100304582"/>
    <dbReference type="CTD" id="8459"/>
    <dbReference type="GeneTree" id="ENSGT00390000006030"/>
    <dbReference type="OMA" id="HNTERVL"/>
    <dbReference type="OrthoDB" id="545675at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008476">
        <property type="term" value="F:protein-tyrosine sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000290">
        <property type="entry name" value="Protein-tyrosine sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026634">
        <property type="entry name" value="TPST-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12788">
        <property type="entry name" value="PROTEIN-TYROSINE SULFOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12788:SF6">
        <property type="entry name" value="PROTEIN-TYROSINE SULFOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13469">
        <property type="entry name" value="Sulfotransfer_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14 18" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 13 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5011323989" description="Protein-tyrosine sulfotransferase" evidence="14 18">
        <location>
            <begin position="21"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAO42985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AHH42822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="NP_001187093.1"/>
        </source>
    </evidence>
    <sequence length="356" mass="40044" checksum="84D6564B4FE073B9" modified="2003-06-01" version="1">MRLSVRRGFILLLCLFGVYAAVKALYHMLMCQSGYPHSLGVSILVKGSNQTLYHYNQDSPMVFVGGVPRSGTTLMRAMLDAHPNIRCGEETRVIPRLLALRHGWRRSAEDRWALDEEEISQDMLDSATAAFLLEVIARHGEPAPLLCNKDPFTLKSSIYLSKLFPNSKFLLMVRDGRASVHSMISRQVTIAGFDLSSYRDCLSKWSQAIEVMLFQCTLVGSTRCLTIRYENLVLQPRTTMVRVLEFLKVPWHEGVLHHEEAIGQPGGVSLSRIERSTDQVIKPVNLEALTRWVGHIPADVLKDMENIAPMLNKLGYDPTANPPQYGQPDPEVIHNTERVLKGDFKPPASLKRPSQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-02-19" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>V5RK41</accession>
    <name>V5RK41_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription factor Jun</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Proto-oncogene c-Jun</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Transcription factor AP-1 subunit Jun</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">jun</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2013-08" db="EMBL/GenBank/DDBJ databases">
            <title>Transcription Factor Expression and Regulation in Innate Immunity of Channel Catfish (Ictalurus punctatus).</title>
            <authorList>
                <person name="Leary J.H.III."/>
                <person name="Jaso-Friedmann L."/>
                <person name="Evans D.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bZIP family. Jun subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="KF572026">
        <property type="protein sequence ID" value="AHB37084.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001316243.1">
        <property type="nucleotide sequence ID" value="NM_001329314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="V5RK41"/>
    <dbReference type="GeneID" id="108258130"/>
    <dbReference type="KEGG" id="ipu:108258130"/>
    <dbReference type="CTD" id="3725"/>
    <dbReference type="OrthoDB" id="2187714at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14696">
        <property type="entry name" value="bZIP_Jun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000012">
        <property type="entry name" value="Putative transcription factor AP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050946">
        <property type="entry name" value="AP-1_TF_bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005643">
        <property type="entry name" value="JNK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002112">
        <property type="entry name" value="Leuzip_Jun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008917">
        <property type="entry name" value="TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11462">
        <property type="entry name" value="JUN TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11462:SF8">
        <property type="entry name" value="TRANSCRIPTION FACTOR JUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03957">
        <property type="entry name" value="Jun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00043">
        <property type="entry name" value="LEUZIPPRJUN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47454">
        <property type="entry name" value="A DNA-binding domain in eukaryotic transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="245"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="250"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AHB37084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="NP_001316243.1"/>
        </source>
    </evidence>
    <sequence length="324" mass="35847" checksum="8EAE438CDCC68275" modified="2014-02-19" version="1">MSTKMETTFYDDSLSGAFSQHDGAAFGYNAKTLKHSMTLNLSDPSSTLKPHLRPKINDILTSPDVGLLKLASPELERLIIQSSNGLITTTPTPTQFLCPKNVTDEQEGFVEGFVRALAELHHQHMPPSSVTTTPQTTINNSMAPVSSIAGGAVYSSTVRSEPPVYADLSTFNPPITTAPSFTSANPAMSSYTTTSMPPQQQQQQQHTMAVQHPRLQALKEEPQTVPEMPGETPPLSLIDMESQERIKAERKRMRNRIAASKCRKRKLERISRLEDKVKTLKSQNSELASTANMLREQVAQLKQKVMNHVNSGCQLMLTQQLQTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5U715</accession>
    <name>W5U715_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Nectin-4</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="15">Poliovirus receptor-related protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">pvrl4</name>
        <name evidence="17" type="synonym">nectin4a</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nectin family.</text>
    </comment>
    <dbReference type="EMBL" id="JT405865">
        <property type="protein sequence ID" value="AHH37359.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017327524.1">
        <property type="nucleotide sequence ID" value="XM_017472035.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U715"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027424"/>
    <dbReference type="GeneID" id="108267696"/>
    <dbReference type="KEGG" id="ipu:108267696"/>
    <dbReference type="CTD" id="560512"/>
    <dbReference type="GeneTree" id="ENSGT00940000157535"/>
    <dbReference type="OMA" id="HFEHTNG"/>
    <dbReference type="OrthoDB" id="8872282at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051427">
        <property type="entry name" value="Nectin/Nectin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23277:SF11">
        <property type="entry name" value="NECTIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23277">
        <property type="entry name" value="NECTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540034" evidence="13">
        <location>
            <begin position="22"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="332"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="27"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="140"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="233"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="419"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="512"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="451"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AHH37359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017327524.1"/>
        </source>
    </evidence>
    <sequence length="633" mass="69781" checksum="DF5D286DB1B5CE18" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5U7A4</accession>
    <name>W5U7A4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">60S ribosomal export protein NMD3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 14" type="primary">nmd3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Acts as an adapter for the XPO1/CRM1-mediated export of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the NMD3 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT406105">
        <property type="protein sequence ID" value="AHH37454.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017351227.1">
        <property type="nucleotide sequence ID" value="XM_017495738.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U7A4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007436"/>
    <dbReference type="GeneID" id="108280593"/>
    <dbReference type="KEGG" id="ipu:108280593"/>
    <dbReference type="CTD" id="51068"/>
    <dbReference type="GeneTree" id="ENSGT00390000005104"/>
    <dbReference type="OMA" id="VILVRKH"/>
    <dbReference type="OrthoDB" id="203821at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039768">
        <property type="entry name" value="Nmd3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007064">
        <property type="entry name" value="Nmd3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048899">
        <property type="entry name" value="NMD_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048898">
        <property type="entry name" value="OB_NMD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746:SF2">
        <property type="entry name" value="60S RIBOSOMAL EXPORT PROTEIN NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04981">
        <property type="entry name" value="NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21193">
        <property type="entry name" value="NMD_SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21192">
        <property type="entry name" value="OB_NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nmd3 N-terminal" evidence="9">
        <location>
            <begin position="17"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="60S ribosomal export protein NMD3 SH3" evidence="11">
        <location>
            <begin position="249"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="60S ribosomal export protein NMD3 OB-fold" evidence="10">
        <location>
            <begin position="313"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AHH37454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017351227.1"/>
        </source>
    </evidence>
    <sequence length="504" mass="57910" checksum="3822FA7B8A1A2F41" modified="2014-04-16" version="1">MEYMQAPASSSQGNILCCTCGIPIPPNPANMCVSCLRTQVDISDGIPKQVSIHFCKQCERYLQPPATWVQCALESRELLTLCLKKIKGLMSKVRLIDAGFVWTEPHSKRIKLKLTIQKEVMNGAILQQVFVVDYIIQSQMCDDCHRVEAKDYWKAVVQVRQKTVHKKTFYYLEQLILKHRVHQNTLRIKEIHEGIDFYYSSKQHAQKMVDFLQCTVPCRSKSSQRLISHDVHSNSYNYKSTFSIEIVPICKDNVVCLSPRLAQSLGNLGQVCVCIQVTSTVHLIDPKTLQLAEIDANTYWRHPFNSLLNPRQLEEFIVMDADIIRDQRLGAGAGLRSNRHTLAEVWVQKTSEMNTSQQYHCRTHLGHLLNIGDLVLGYDFANSNLNDEFLNKMNPHHVPDVVLIKKSYDRTKRIKRRNWKLKELHRDREGTDTDDERQYQDFLEDLEEDEALRKNVNIFKDISKIPVESDTDDEGIPRISLAEMMEDLSLSDATGGEGADMMTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5U7B0</accession>
    <name>W5U7B0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Phosphomannomutase</fullName>
            <ecNumber evidence="5 13">5.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PMM1</name>
        <name evidence="16" type="synonym">LOC108272846</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>alpha-D-mannose 1-phosphate = D-mannose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11140"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="EC" id="5.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the eukaryotic PMM family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.8" evidence="5 13"/>
    <dbReference type="EMBL" id="JT406117">
        <property type="protein sequence ID" value="AHH37459.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017337135.1">
        <property type="nucleotide sequence ID" value="XM_017481646.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U7B0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029202"/>
    <dbReference type="GeneID" id="108272846"/>
    <dbReference type="KEGG" id="ipu:108272846"/>
    <dbReference type="CTD" id="5372"/>
    <dbReference type="OMA" id="FCLHYMA"/>
    <dbReference type="OrthoDB" id="10264771at2759"/>
    <dbReference type="UniPathway" id="UPA00126">
        <property type="reaction ID" value="UER00424"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004615">
        <property type="term" value="F:phosphomannomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02585">
        <property type="entry name" value="HAD_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1240.20:FF:000001">
        <property type="entry name" value="Phosphomannomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005002">
        <property type="entry name" value="PMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043169">
        <property type="entry name" value="PMM_cap"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10466">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10466:SF1">
        <property type="entry name" value="PHOSPHOMANNOMUTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03332">
        <property type="entry name" value="PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00445">
        <property type="entry name" value="alpha-phosphomannomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01143">
        <property type="entry name" value="C2.B.3:_Phosphomannomutase_Lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01140">
        <property type="entry name" value="C2.B:_Phosphomannomutase_and_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AHH37459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017337135.1"/>
        </source>
    </evidence>
    <sequence length="259" mass="29595" checksum="A214AA34FACE0FDB" modified="2014-04-16" version="1">MERRNLPDRDVLCLFDVDGTLTLPREKIDPQLDAFFQSLRSKVKIGIVGGSDYSKIAEQLGEGDDVIHKFDYVFAENGTVQYKDGRLLSKQAIQNQLGEELLQDLINFCLRYMGLIKLPKKRGTFIEFRNGMLNISPVGRSCTLEERIEFAEIDKKEKIREKFVAALQKEFAGKGLRFTRGGLISFDIFPEGWDKRLCLDVLEQEGLSTIYFFGNETSLGGNDYEIFEDPRTIGFTVYSPKDTVRLCQELFFTTPPNEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5U7R4</accession>
    <name>W5U7R4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dihydrofolate reductase</fullName>
            <ecNumber evidence="3">1.5.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Dhfr</name>
        <name evidence="11" type="synonym">dhfr</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15009"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dihydrofolate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.3" evidence="3"/>
    <dbReference type="EMBL" id="JT406487">
        <property type="protein sequence ID" value="AHH37609.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017307175.1">
        <property type="nucleotide sequence ID" value="XM_017451686.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U7R4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034016"/>
    <dbReference type="GeneID" id="108255600"/>
    <dbReference type="KEGG" id="ipu:108255600"/>
    <dbReference type="CTD" id="1719"/>
    <dbReference type="OMA" id="RDNQLPW"/>
    <dbReference type="OrthoDB" id="4664297at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004146">
        <property type="term" value="F:dihydrofolate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046452">
        <property type="term" value="P:dihydrofolate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046655">
        <property type="term" value="P:folic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00209">
        <property type="entry name" value="DHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.430.10:FF:000002">
        <property type="entry name" value="Dihydrofolate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="entry name" value="Dihydrofolate Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012259">
        <property type="entry name" value="DHFR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001796">
        <property type="entry name" value="DHFR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48069">
        <property type="entry name" value="DIHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48069:SF6">
        <property type="entry name" value="DIHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00186">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00070">
        <property type="entry name" value="DHFR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="Dihydrofolate reductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51330">
        <property type="entry name" value="DHFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHFR" evidence="8">
        <location>
            <begin position="4"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AHH37609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017307175.1"/>
        </source>
    </evidence>
    <sequence length="190" mass="21577" checksum="3C3A48A1CA631482" modified="2014-04-16" version="1">MGRVLNCIVAVCPDMGIGRNGNLPWHPIRLSKEFKHFQKMTMTPTVEGKKNVVIMGRKTWFSIPAQNRPLKNRINVVLSRELKTAPEGAHYLAADFSSALRILENAELEAQADQVWIIGGSSLYKEVMESSAHQRLFVTRILKQFDCDTFIPNIDLDKFRLLPEFPGVPAGLQEENGLQYVFEVYESVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5U834</accession>
    <name>W5U834_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8 10">Heat shock 70 kDa protein 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">hspa14</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT406759">
        <property type="protein sequence ID" value="AHH37714.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017350154.1">
        <property type="nucleotide sequence ID" value="XM_017494665.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U834"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029163"/>
    <dbReference type="GeneID" id="108279968"/>
    <dbReference type="KEGG" id="ipu:108279968"/>
    <dbReference type="CTD" id="51182"/>
    <dbReference type="OMA" id="DQVLMDH"/>
    <dbReference type="OrthoDB" id="29851at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10238">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000013">
        <property type="entry name" value="Heat shock 70 kDa protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000008">
        <property type="entry name" value="heat shock 70 kDa protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000010">
        <property type="entry name" value="heat shock 70 kDa protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000171">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000433">
        <property type="entry name" value="Heat shock protein family A (Hsp70) member 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042049">
        <property type="entry name" value="HSPA14_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0346">Stress response</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AHH37714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017350154.1"/>
        </source>
    </evidence>
    <sequence length="504" mass="54212" checksum="A650F79F649A6306" modified="2014-04-16" version="1">MAAIGVHFGYTCACVAVFKDGRADVVANDAGDRVTPAVVAYRETEQIVGIAAKQGRIRNAANTVVKVKQILGRCYDDPEAQAHRDESKCTIVKKGDLPMYELDMGETTKYVSPEDVAKLVFHKIKETAQSALGSDVKDAVITVPFEFGEMQKKALRNAAEGAGFNVLRLIHEPSAALLAYGIGQDTPSGKSHVLVYKLGGTSLSVTALEVNSGMYRVLATHTDHSTGGESFTQALAQYLAGEFKKLYKHDVSGNLRAMMKLMNSADVAKHTLSTLGSSNCFVDSLYDGVDFECNVSRARFELICSNLFSKSIQPIRKLLEQVNLSTTDVNKVVLSGGSARIPKLQQMIKDLFPDVELLCSIPPDEVIPVGAAMQAGILVGKDSLDLGEESITVDCCASDILVKEVDESGADLFTVLFPSGTPLPARRQHTLQGPGSLVSVCLELYQAQKPLAQIVLRDLEPKEEMHDIVTVVTMKRDGSLHVTCTEQGSGRSEAVTIETAAAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5U8B5</accession>
    <name>W5U8B5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">F-box only protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">FBXO11</name>
        <name evidence="19" type="synonym">fbxo11b</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the SCF(FBXO11) complex consisting of CUL1, RBX1, SKP1 and FBXO11. Interacts with CIITA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT406904">
        <property type="protein sequence ID" value="AHH37774.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017339658.1">
        <property type="nucleotide sequence ID" value="XM_017484169.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U8B5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012753"/>
    <dbReference type="GeneID" id="108274184"/>
    <dbReference type="KEGG" id="ipu:108274184"/>
    <dbReference type="CTD" id="567742"/>
    <dbReference type="OrthoDB" id="427974at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22091">
        <property type="entry name" value="F-box_FBXO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19676">
        <property type="entry name" value="UBR-box_UBR6_FBXO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000003">
        <property type="entry name" value="F-box only protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000005">
        <property type="entry name" value="F-box only protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000006">
        <property type="entry name" value="F-box only protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000007">
        <property type="entry name" value="F-box only protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006633">
        <property type="entry name" value="Carb-bd_sugar_hydrolysis-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047505">
        <property type="entry name" value="F-box_FBXO11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047504">
        <property type="entry name" value="FBXO11_UBR-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022441">
        <property type="entry name" value="Para_beta_helix_rpt-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051550">
        <property type="entry name" value="SCF-Subunits/Alg-Epimerases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03804">
        <property type="entry name" value="para_beta_helix"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22990">
        <property type="entry name" value="F-BOX ONLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22990:SF20">
        <property type="entry name" value="F-BOX ONLY PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00722">
        <property type="entry name" value="CASH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="F-box" evidence="15">
        <location>
            <begin position="105"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="UBR-type" evidence="16">
        <location>
            <begin position="785"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="13">
        <location>
            <begin position="785"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="64"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00063474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AHH37774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017339658.1"/>
        </source>
    </evidence>
    <sequence length="879" mass="98203" checksum="982F2473D3AE9742" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5U9H5</accession>
    <name>W5U9H5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8 10">Protein MANBAL</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MANBAL</name>
        <name evidence="10" type="synonym">manbal</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0239 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT408022">
        <property type="protein sequence ID" value="AHH38209.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017334643.1">
        <property type="nucleotide sequence ID" value="XM_017479154.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U9H5"/>
    <dbReference type="GeneID" id="108271526"/>
    <dbReference type="KEGG" id="ipu:108271526"/>
    <dbReference type="CTD" id="63905"/>
    <dbReference type="GeneTree" id="ENSGT00390000003422"/>
    <dbReference type="OMA" id="CEARPER"/>
    <dbReference type="OrthoDB" id="10040809at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009621">
        <property type="entry name" value="UPF0239"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14409">
        <property type="entry name" value="MANNOSIDASE, BETA A, LYSOSOMAL-LIKE, MANBAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14409:SF0">
        <property type="entry name" value="PROTEIN MANBAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06783">
        <property type="entry name" value="UPF0239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="46"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AHH38209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017334643.1"/>
        </source>
    </evidence>
    <sequence length="87" mass="9749" checksum="674B8F17E90A3556" modified="2014-04-16" version="1">MSGDLDLSPPEVPEPTFFESVLRYGLFLGAIFQLVCILAIIIPSSKSHEQEETSEPAEPKSSEPSRKPKAPAQQIRQKLKKESKKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5UB02</accession>
    <name>W5UB02_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14 16 17">Ectonucleotide pyrophosphatase/phosphodiesterase family member 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">Enpp5</name>
        <name evidence="16 17 18" type="synonym">enpp5</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16 17 18" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16 17 18">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="JT410857">
        <property type="protein sequence ID" value="AHH39211.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017311901.1">
        <property type="nucleotide sequence ID" value="XM_017456412.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053531571.1">
        <property type="nucleotide sequence ID" value="XM_053675596.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053531572.1">
        <property type="nucleotide sequence ID" value="XM_053675597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UB02"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000023678"/>
    <dbReference type="GeneID" id="108258082"/>
    <dbReference type="KEGG" id="ipu:108258082"/>
    <dbReference type="CTD" id="59084"/>
    <dbReference type="GeneTree" id="ENSGT00940000160562"/>
    <dbReference type="OMA" id="DEYVSRD"/>
    <dbReference type="OrthoDB" id="415411at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16018">
        <property type="entry name" value="Enpp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.180:FF:000004">
        <property type="entry name" value="Ectonucleotide pyrophosphatase/phosphodiesterase family member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF125">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE_PHOSPHODIESTERASE FAMILY MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540118" evidence="13">
        <location>
            <begin position="27"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="420"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AHH39211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017311901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_053531571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_053531572.1"/>
        </source>
    </evidence>
    <sequence length="470" mass="53655" checksum="F78F14B2987AD399" modified="2014-04-16" version="1">MNVVLMRKMYLLSCFLALLLPPLSHQEEHHKLLLVSFDGFRWDYVNRVPTPNFRALMDEGVQVERVENTYITKTFPNHYTLVTGLHAESHGIVANEMYDPVLNRSFSMDGPEVYDSRWWEEAVPLWVTNQMAGRRSGAAMWPGSDVSIGGIYPTRYMPYNASMPFEVRVEKLISWFSGPGAINFGVLYWEEPDESGHNLGPESPLMDVVIEDIDVKLGFLRDELRRAGLYDKINLIVTSDHGMTQLSRDKVIELDTYVSRELYTWIDKSPVVGILPKEGKFDEVYNLLENANPNMVVYKREDIPDHFHYKHNVRIMPIIIEVKEGWTIVQNKTGSFMLGNHGYDNHLPSMHPVFVARGPSFRTGYTKASMRSVDLYPLMCSVLGIRALPNNGSLMSVRDLLVETFTPRPVPPPIPKEPSYAWAVRTVLGSGLVLGFLIIFVKQLTQRQLPPLPLTNREISQPLLKDELHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5UB94</accession>
    <name>W5UB94_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein 138</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15 17 18 19 20 21 22" type="primary">tmem138</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17 18 19" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17 18 19">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for ciliogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Vacuole membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TMEM138 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT411001">
        <property type="protein sequence ID" value="AHH39271.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053541676.1">
        <property type="nucleotide sequence ID" value="XM_053685701.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053541677.1">
        <property type="nucleotide sequence ID" value="XM_053685702.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053541678.1">
        <property type="nucleotide sequence ID" value="XM_053685703.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053541679.1">
        <property type="nucleotide sequence ID" value="XM_053685704.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053541680.1">
        <property type="nucleotide sequence ID" value="XM_053685705.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053541681.1">
        <property type="nucleotide sequence ID" value="XM_053685706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UB94"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020101"/>
    <dbReference type="GeneID" id="108274734"/>
    <dbReference type="KEGG" id="ipu:108274734"/>
    <dbReference type="CTD" id="51524"/>
    <dbReference type="GeneTree" id="ENSGT00390000018587"/>
    <dbReference type="OMA" id="FYKRTAM"/>
    <dbReference type="OrthoDB" id="189688at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024133">
        <property type="entry name" value="TM_138"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13306">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 138"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13306:SF6">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 138"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14935">
        <property type="entry name" value="TMEM138"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AHH39271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_053541676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_053541677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_053541678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_053541679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_053541680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_053541681.1"/>
        </source>
    </evidence>
    <sequence length="162" mass="19179" checksum="B4A84A7CD93966F6" modified="2014-04-16" version="1">MLQTNNYSLVLLIQLSLLAFDLFVNSFCELLRTAPVIQLVLFIIQDIAILFNVIIILLMMFNTYVFQVGLLSLLLERFRALLILSAVYLTLSICLHCWLMNLRWMESNRFVWTDGLQVLFVFQRLAAVLYYYLYKRSTEYLGDPRLYEDCPWLRDAFARARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5UBC4</accession>
    <name>W5UBC4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5 7 8">Zinc finger and BTB domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ZBTB3</name>
        <name evidence="7 8" type="synonym">zbtb3</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7 8">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT409938">
        <property type="protein sequence ID" value="AHH38879.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017329496.1">
        <property type="nucleotide sequence ID" value="XM_017474007.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047013048.1">
        <property type="nucleotide sequence ID" value="XM_047157092.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UBC4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026684"/>
    <dbReference type="GeneID" id="108268802"/>
    <dbReference type="KEGG" id="ipu:108268802"/>
    <dbReference type="CTD" id="79842"/>
    <dbReference type="GeneTree" id="ENSGT00940000161486"/>
    <dbReference type="OMA" id="YPRGLVT"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000114">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000892">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF28">
        <property type="entry name" value="ZINC FINGER PROTEIN 37-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="24"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="4">
        <location>
            <begin position="507"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="4">
        <location>
            <begin position="535"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="131"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="357"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="453"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="561"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="160"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="181"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="244"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="278"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="463"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="561"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="572"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AHH38879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017329496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_047013048.1"/>
        </source>
    </evidence>
    <sequence length="585" mass="63637" checksum="CAFBB35CF020BC6E" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5UC23</accession>
    <name>W5UC23_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Fibroblast growth factor</fullName>
            <shortName evidence="9">FGF</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">FGF6</name>
        <name evidence="12" type="synonym">fgf6a</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="EMBL" id="JT410620">
        <property type="protein sequence ID" value="AHH39134.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053538212.1">
        <property type="nucleotide sequence ID" value="XM_053682237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UC23"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000017352"/>
    <dbReference type="GeneTree" id="ENSGT00940000157821"/>
    <dbReference type="OMA" id="GAHDENP"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23318">
        <property type="entry name" value="beta-trefoil_FGF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000033">
        <property type="entry name" value="Fibroblast growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00263">
        <property type="entry name" value="HBGFFGF"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00442">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00247">
        <property type="entry name" value="HBGF_FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7" id="KW-0339">Growth factor</keyword>
    <keyword evidence="8" id="KW-0497">Mitogen</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU049442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AHH39134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053538212.1"/>
        </source>
    </evidence>
    <sequence length="191" mass="21648" checksum="6CCAB036EC8F0814" modified="2014-04-16" version="1">MPRRESSARHTSTATARTAIVLLGFLLGVASSRRTHATTLEARWETLFARSVLGISGDNDYLMGVKRVRRLYCNVGIGFHLQVLPDGRINGVHDENQYSLIEISAVERGVVSLYGLKSELFVAMNRRGRLYATRDFRDECKFKETLLPNNYNAYESSIYKGFYIALSKHGRLKRGYKATPAMTVTHFLPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5UCT5</accession>
    <name>W5UCT5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6 8 9">14-3-3 protein zeta/delta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">YWHAZ</name>
        <name evidence="8 9 10" type="synonym">ywhaz</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8 9 10" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8 9 10">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer, and heterodimer with other family members.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the 14-3-3 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT412179">
        <property type="protein sequence ID" value="AHH39743.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017310439.1">
        <property type="nucleotide sequence ID" value="XM_017454950.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017310440.1">
        <property type="nucleotide sequence ID" value="XM_017454951.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047006480.1">
        <property type="nucleotide sequence ID" value="XM_047150524.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UCT5"/>
    <dbReference type="SMR" id="W5UCT5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000015867"/>
    <dbReference type="GeneID" id="108257302"/>
    <dbReference type="KEGG" id="ipu:108257302"/>
    <dbReference type="CTD" id="7534"/>
    <dbReference type="GeneTree" id="ENSGT01090000260040"/>
    <dbReference type="OMA" id="DISTENM"/>
    <dbReference type="OrthoDB" id="10260625at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.190.20:FF:000001">
        <property type="entry name" value="14-3-3 gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023409">
        <property type="entry name" value="14-3-3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00796">
        <property type="entry name" value="1433_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00797">
        <property type="entry name" value="1433_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="5">
        <location>
            <begin position="3"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="3">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="3">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000868-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AHH39743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017310439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017310440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_047006480.1"/>
        </source>
    </evidence>
    <sequence length="243" mass="27932" checksum="B53CC7170C7C46AA" modified="2014-04-16" version="1">MDKSELVQKAKLAEQAERYDDMASVMKEVTEKGEELTDEERNLLSVAYKNVVGACRSAWRVVSSIEQKMKLESSDKEQIAKEYRENIESELQTICNDVLHLLDKFLIRTSSPAESQVFYLKMKGDYYRYLAEVAVGDDKKSIVDKSREAYQAAFDISTENMQPTHPIRLGLALNFSVFYYEILGSREQACELAKKAFDDAIAELDQLTEDSYKDSTLIMQLLRDNLTLWTSDNQEETEEGREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5UEH4</accession>
    <name>W5UEH4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 12">Occludin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">OCLN</name>
        <name evidence="23" type="synonym">si:ch73-61d6.3</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cell junction</location>
            <location evidence="12">Tight junction</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 12 15">Belongs to the ELL/occludin family.</text>
    </comment>
    <dbReference type="EMBL" id="JT412762">
        <property type="protein sequence ID" value="AHH39984.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017322992.1">
        <property type="nucleotide sequence ID" value="XM_017467503.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UEH4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026249"/>
    <dbReference type="GeneID" id="108265315"/>
    <dbReference type="KEGG" id="ipu:108265315"/>
    <dbReference type="GeneTree" id="ENSGT00940000165052"/>
    <dbReference type="OMA" id="AFFAHKT"/>
    <dbReference type="OrthoDB" id="8867927at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070830">
        <property type="term" value="P:bicellular tight junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031176">
        <property type="entry name" value="ELL/occludin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002958">
        <property type="entry name" value="Occludin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010844">
        <property type="entry name" value="Occludin_ELL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288:SF6">
        <property type="entry name" value="OCCLUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288">
        <property type="entry name" value="OCCLUDIN AND RNA POLYMERASE II ELONGATION FACTOR ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07303">
        <property type="entry name" value="Occludin_ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005993">
        <property type="entry name" value="Occludin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01258">
        <property type="entry name" value="OCCLUDIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144292">
        <property type="entry name" value="occludin/ELL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51980">
        <property type="entry name" value="OCEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7 12" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0796">Tight junction</keyword>
    <keyword evidence="6 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="19">
        <location>
            <begin position="58"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="OCEL" evidence="20">
        <location>
            <begin position="407"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="352"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="436"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="215"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR005993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR005993-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="AHH39984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017322992.1"/>
        </source>
    </evidence>
    <sequence length="515" mass="58138" checksum="EF27EF00007C9FFF" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5UEW1</accession>
    <name>W5UEW1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5 7">Epoxide hydrolase 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Ephx4</name>
        <name evidence="7" type="synonym">ephx4</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="JT413084">
        <property type="protein sequence ID" value="AHH40104.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017335344.1">
        <property type="nucleotide sequence ID" value="XM_017479855.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UEW1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018346"/>
    <dbReference type="GeneID" id="108271913"/>
    <dbReference type="KEGG" id="ipu:108271913"/>
    <dbReference type="CTD" id="253152"/>
    <dbReference type="GeneTree" id="ENSGT00940000156233"/>
    <dbReference type="OMA" id="YRWMVRS"/>
    <dbReference type="OrthoDB" id="408373at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004301">
        <property type="term" value="F:epoxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43329">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1 5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="98"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AHH40104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017335344.1"/>
        </source>
    </evidence>
    <sequence length="369" mass="42758" checksum="A83D4ABD3D6DF66A" modified="2014-04-16" version="1">MARLLGRVLLLSVRLAVKLRAAGYWTLIYGCCGLCAVLALLRLCWNVLMRPTATFRWTVRETPPPCLNDTSLGTHCYVRIKESGLRFHYVAAGERGKPLMLFLHGFPEFWFSWRHQLREFKSEFRVVAVDMRGYGESDLPPSTECYKLDCLVTDIKDIVEYLGYNRCFLVGHDWGGIIAWLFAIHYPEMVVKLVVLNSPHPCVFADYALRHPSQMLKSSYFFFFQLPRFPEFMLSINDFKALKSLFTSRSTGIGLKGRWLTAEELEAYLYALSQPGALTAALNYYRNVFSCFPLNQNEVRSPVLLLWGERDAFLEQEMAEACRLYIRNHFRLNIISGASHWLQQDQPDIVNTLIWTFLKEGEGRKNYRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5UG80</accession>
    <name>W5UG80_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Alpha/beta hydrolase domain-containing protein 14A</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="7 8">Protein ABHD14A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 7 8" type="primary">abhd14a</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7 8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AB hydrolase superfamily. ABHD14 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT414308">
        <property type="protein sequence ID" value="AHH40574.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017305970.1">
        <property type="nucleotide sequence ID" value="XM_017450481.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017305971.1">
        <property type="nucleotide sequence ID" value="XM_017450482.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UG80"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000028966"/>
    <dbReference type="GeneID" id="108254949"/>
    <dbReference type="KEGG" id="ipu:108254949"/>
    <dbReference type="CTD" id="25864"/>
    <dbReference type="OMA" id="IQLPNHE"/>
    <dbReference type="OrthoDB" id="284184at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000077">
        <property type="entry name" value="Abhydrolase domain containing 14B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46197:SF1">
        <property type="entry name" value="PROTEIN ABHD14A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46197">
        <property type="entry name" value="PROTEIN ABHD14B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="90"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AHH40574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017305970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017305971.1"/>
        </source>
    </evidence>
    <sequence length="264" mass="29325" checksum="27D072DA5321DDC1" modified="2014-04-16" version="1">MSFLRNRAVVLGLVLLFTVLLYLLLPSSIRQSNTEPSLAVQRSQRLISASPLPINITVRTGQIPSDPPLFFREALPVDLSGRQVLPRLQVVLLHGQAFTSRTWEELGTLSLLAANRYQALALDLPGFGKSPASESVKSEQSRVDLLKHFLEALGVRAPVLITPSMSGHYALPFLQRFSDQLRAFIPIAPVATRTLTPQQYHDIQTPTLIVVGELDTNLGSQSLKNLQHLPHHSVVKLAGARHACYMDKPREFHQALLHFLNTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5UJD9</accession>
    <name>W5UJD9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein disulfide-isomerase A5</fullName>
            <ecNumber evidence="3">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PDIA5</name>
        <name evidence="14" type="synonym">pdia5</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="3"/>
    <dbReference type="EMBL" id="JT412217">
        <property type="protein sequence ID" value="AHH39757.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017324996.1">
        <property type="nucleotide sequence ID" value="XM_017469507.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UJD9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000714"/>
    <dbReference type="GeneID" id="108266349"/>
    <dbReference type="KEGG" id="ipu:108266349"/>
    <dbReference type="CTD" id="10954"/>
    <dbReference type="GeneTree" id="ENSGT00940000156797"/>
    <dbReference type="OrthoDB" id="74910at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02997">
        <property type="entry name" value="PDI_a_PDIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd03067">
        <property type="entry name" value="PDI_b_PDIR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000029">
        <property type="entry name" value="protein disulfide-isomerase A5 isoform X2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000105">
        <property type="entry name" value="protein disulfide-isomerase A5 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051063">
        <property type="entry name" value="PDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046374">
        <property type="entry name" value="PDI_a_PDIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041865">
        <property type="entry name" value="PDI_b_PDIR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672:SF2">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE C17H9.14C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
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        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="143"/>
            <end position="269"/>
        </location>
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    <feature type="domain" description="Thioredoxin" evidence="11">
        <location>
            <begin position="275"/>
            <end position="386"/>
        </location>
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    <feature type="domain" description="Thioredoxin" evidence="11">
        <location>
            <begin position="387"/>
            <end position="515"/>
        </location>
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        <location>
            <begin position="389"/>
            <end position="408"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AHH39757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017324996.1"/>
        </source>
    </evidence>
    <sequence length="528" mass="60494" checksum="9DF27217221A2F02" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5UJG7</accession>
    <name>W5UJG7_ICTPU</name>
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        <submittedName>
            <fullName evidence="10">E3 ubiquitin-protein ligase RNF113A</fullName>
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        <submittedName>
            <fullName evidence="8">RING finger protein 113A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">RNF113A</name>
        <name evidence="10" type="synonym">rnf113a</name>
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    <organism evidence="8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
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            <taxon>Ictaluridae</taxon>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
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    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT417187">
        <property type="protein sequence ID" value="AHH42289.1"/>
        <property type="molecule type" value="mRNA"/>
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        <property type="nucleotide sequence ID" value="XM_017459101.3"/>
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    <dbReference type="STRING" id="7998.ENSIPUP00000019807"/>
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    <dbReference type="KEGG" id="ipu:108259520"/>
    <dbReference type="CTD" id="7737"/>
    <dbReference type="GeneTree" id="ENSGT00390000016292"/>
    <dbReference type="OMA" id="WQLEADH"/>
    <dbReference type="OrthoDB" id="25761at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034247">
        <property type="term" value="P:snoRNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16539">
        <property type="entry name" value="RING-HC_RNF113A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING finger protein 113A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039971">
        <property type="entry name" value="CWC24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12930:SF0">
        <property type="entry name" value="RING FINGER PROTEIN 113B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12930">
        <property type="entry name" value="ZINC FINGER PROTEIN 183"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="185"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="251"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="185"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="21"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AHH42289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017314590.1"/>
        </source>
    </evidence>
    <sequence length="322" mass="37100" checksum="250304D465A3AB18" modified="2014-04-16" version="1">MAETEETKPATCTFLFKKSSKKFSARKRKASDSDKDRSSDEEKNAVVKREKKPSSHNPMIQRTKKVEKEVVSSSESDEDKEAKKITVAYKSTRSAKPEGPDDMGATAVYELDTERDKDAQAIFERSQKIQEELQGKEDDKIYRGINNYQKFIKPKDTTMGNASSGMVRKGPIRAPEHLRATVRWDYQPDICKDYKETGFCGFGDSCKFLHDRSDYKHGWQIERELEEGRYGANDEENYEVSSDDEDLPFKCFICRDSFKNPIITKCRHYFCEACALQHYRKSKRCYVCNQQTNGVFNPAKELMAKMQKREAAGDQPPSDEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5UJV4</accession>
    <name>W5UJV4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10 12">Transcriptional activator protein Pur-alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PURA</name>
        <name evidence="12" type="synonym">purab</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PUR DNA-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="JT412707">
        <property type="protein sequence ID" value="AHH39962.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017329564.1">
        <property type="nucleotide sequence ID" value="XM_017474075.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UJV4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003073"/>
    <dbReference type="GeneID" id="108268831"/>
    <dbReference type="KEGG" id="ipu:108268831"/>
    <dbReference type="CTD" id="557266"/>
    <dbReference type="GeneTree" id="ENSGT00950000183162"/>
    <dbReference type="OMA" id="TIPEHHW"/>
    <dbReference type="OrthoDB" id="523901at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032422">
        <property type="term" value="F:purine-rich negative regulatory element binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.700:FF:000001">
        <property type="entry name" value="Purine-rich element binding protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2450.30:FF:000001">
        <property type="entry name" value="Purine-rich element binding protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2450.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006628">
        <property type="entry name" value="PUR-bd_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12611">
        <property type="entry name" value="PUR-TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12611:SF5">
        <property type="entry name" value="PURINE-RICH ELEMENT-BINDING PROTEIN AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04845">
        <property type="entry name" value="PurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00712">
        <property type="entry name" value="PUR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AHH39962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017329564.1"/>
        </source>
    </evidence>
    <sequence length="282" mass="31685" checksum="85D57608E5A20EFA" modified="2014-04-16" version="1">MADRDSGSEHGGFATGPGGGPMHQGAMGASSRLQHDTEELASKRVDIQNKRFYLDVKQNAKGRFLKIAEVGAAGNKSRLTLSMSVAVEFRDYLGDFIEHYAQLGPSHPDTVQDEPRRALKSEFLVRENRKYYMDLKENQRGRFLRIRQTVNRGPGLGTAQGQTIALPAQGLIEFRDALAKLIDDYGVDEDPAELPEGTSLTVDNKRFFFDVGSNKYGVFMRVSEVKPTYRNSITVPCKVWSKFGATFCKYADEMKKIQERSRERRARELLPEALHGDDGEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5UJY8</accession>
    <name>W5UJY8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11 13 14">Growth factor receptor-bound protein 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">GRB10</name>
        <name evidence="13 14" type="synonym">LOC108273075</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13 14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GRB7/10/14 family.</text>
    </comment>
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        <property type="protein sequence ID" value="AHH42469.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_053684097.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053540073.1">
        <property type="nucleotide sequence ID" value="XM_053684098.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UJY8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000015738"/>
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        <property type="component" value="Chromosome 12"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:insulin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008286">
        <property type="term" value="P:insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046627">
        <property type="term" value="P:negative regulation of insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01259">
        <property type="entry name" value="PH_APBB1IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10415">
        <property type="entry name" value="SH2_Grb10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000062">
        <property type="entry name" value="growth factor receptor-bound protein 10 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000056">
        <property type="entry name" value="growth factor receptor-bound protein 10 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000015">
        <property type="entry name" value="Growth factor receptor-bound protein 10 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015042">
        <property type="entry name" value="BPS-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039664">
        <property type="entry name" value="GRB/APBB1IP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035037">
        <property type="entry name" value="Grb10_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039665">
        <property type="entry name" value="PH_APBB1IP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11243">
        <property type="entry name" value="GROWTH FACTOR RECEPTOR-BOUND PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11243:SF4">
        <property type="entry name" value="GROWTH FACTOR RECEPTOR-BOUND PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08947">
        <property type="entry name" value="BPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21989">
        <property type="entry name" value="RA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11 13 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="Ras-associating" evidence="10">
        <location>
            <begin position="171"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="9">
        <location>
            <begin position="297"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="8">
        <location>
            <begin position="500"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="465"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="479"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AHH42469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_053540072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_053540073.1"/>
        </source>
    </evidence>
    <sequence length="601" mass="68348" checksum="1DF5B67FD689F93B" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>W5UML4</accession>
    <name>W5UML4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Sodium/potassium-transporting ATPase subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">atp1a3</name>
        <name evidence="27" type="synonym">atp1a3a</name>
    </gene>
    <organism evidence="25">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="25">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">SDA103</strain>
        </source>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="27">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 22">Cell membrane</location>
            <topology evidence="1 22">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 22">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="JT419121">
        <property type="protein sequence ID" value="AHH42965.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053540157.1">
        <property type="nucleotide sequence ID" value="XM_053684182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UML4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007060"/>
    <dbReference type="KEGG" id="ipu:108272709"/>
    <dbReference type="OMA" id="WFDERIV"/>
    <dbReference type="OrthoDB" id="158672at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005391">
        <property type="term" value="F:P-type sodium:potassium-exchanging transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02608">
        <property type="entry name" value="P-type_ATPase_Na-K_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000106">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000001">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000004">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000095">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050510">
        <property type="entry name" value="Cation_transp_ATPase_P-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005775">
        <property type="entry name" value="P-type_ATPase_IIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01106">
        <property type="entry name" value="ATPase-IIC_X-K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294">
        <property type="entry name" value="SODIUM/POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294:SF15">
        <property type="entry name" value="SODIUM_POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00121">
        <property type="entry name" value="NAKATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="11 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17 22" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13 22" id="KW-0630">Potassium</keyword>
    <keyword evidence="5 22" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0915">Sodium</keyword>
    <keyword evidence="19" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7" id="KW-0740">Sodium/potassium transport</keyword>
    <keyword evidence="14" id="KW-1278">Translocase</keyword>
    <keyword evidence="8 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 22" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="292"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="322"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="777"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="803"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="849"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="914"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="954"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="986"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="24">
        <location>
            <begin position="43"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="13"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00037422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00038795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU362084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="AHH42965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_053540157.1"/>
        </source>
    </evidence>
    <sequence length="1024" mass="112897" checksum="5C62064C94029E45" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5UQT0</accession>
    <name>W5UQT0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Nerve growth factor receptor b</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="17">Tumor necrosis factor receptor superfamily member 16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">NGFR</name>
        <name evidence="19" type="synonym">ngfrb</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
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            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
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            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
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        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
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            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
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        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    </evidence>
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<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
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    <name>W5URL0_ICTPU</name>
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        <name type="scientific">Ictalurus punctatus</name>
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            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
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                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
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            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Mixed</tissue>
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    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Mediates the hydrolysis of oxidized nucleoside diphosphate derivatives. Hydrolyzes 8-oxo-7,8-dihydroguanine (8-oxo-Gua)-containing deoxyribo- and ribonucleoside diphosphates to the monophosphates. Hydrolyzes 8-oxo-dGDP and 8-oxo-GDP with the same efficiencies. Also hydrolyzes 8-OH-dADP and 2-OH-dADP. Exhibited no or minimal hydrolysis activity against 8-oxo-dGTP, 8-oxo-GTP, dGTP, GTP, dGDP and GDP. Probably removes oxidized guanine nucleotides from both the DNA and RNA precursor pools.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>2-oxo-dADP + H2O = 2-oxo-dAMP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35223"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:63212"/>
            <dbReference type="ChEBI" id="CHEBI:71363"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:35224"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>8-oxo-GDP + H2O = 8-oxo-GMP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:62356"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:143554"/>
            <dbReference type="ChEBI" id="CHEBI:145694"/>
            <dbReference type="EC" id="3.6.1.58"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:62357"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>8-oxo-dADP + H2O = 8-oxo-dAMP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35219"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:71361"/>
            <dbReference type="ChEBI" id="CHEBI:71362"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:35220"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>8-oxo-dGDP + H2O = 8-oxo-dGMP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32063"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:63224"/>
            <dbReference type="ChEBI" id="CHEBI:63715"/>
            <dbReference type="EC" id="3.6.1.58"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:32064"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.58" evidence="13"/>
    <dbReference type="EMBL" id="JT417090">
        <property type="protein sequence ID" value="AHH42253.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017324130.1">
        <property type="nucleotide sequence ID" value="XM_017468641.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5URL0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007894"/>
    <dbReference type="GeneID" id="108265859"/>
    <dbReference type="KEGG" id="ipu:108265859"/>
    <dbReference type="CTD" id="79873"/>
    <dbReference type="GeneTree" id="ENSGT00390000002931"/>
    <dbReference type="OMA" id="FPTCEIN"/>
    <dbReference type="OrthoDB" id="10005910at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044715">
        <property type="term" value="F:8-oxo-dGDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044716">
        <property type="term" value="F:8-oxo-GDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04671">
        <property type="entry name" value="NUDIX_8DGDPP_Nudt18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000080">
        <property type="entry name" value="8-oxo-dGDP phosphatase NUDT18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042970">
        <property type="entry name" value="NUDT18_NUDIX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22769:SF56">
        <property type="entry name" value="8-OXO-DGDP PHOSPHATASE NUDT18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22769">
        <property type="entry name" value="MUTT/NUDIX HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="19">
        <location>
            <begin position="41"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00050269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00066482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00071481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00076305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00080473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00083158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="AHH42253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017324130.1"/>
        </source>
    </evidence>
    <sequence length="328" mass="37289" checksum="48ADDE6FCC6B1393" modified="2014-04-16" version="1">MDSVDEDVEEKVEKMLNGEGLEVLDIDAVPDHVQAVTLKKNICYIVSAVIFNSEGQVLMVQEAKRECYGLWYLPAGRMEAGESIHEALRREVKEETGFECEPITMLLVQEQGRQWIRFAFLAKVTGGRLKTPSEADLESLQARWWDREAPLPLRSQDILCLIDAGLRYRQKPWFPVSHPVNLPCSVVCQRQLLIFGCEERIWLLLAKHATDPETRTRFPVTVSQKGYSVKCAGQRLVRECMRSRDHELHVNVHGILGVQHDGRVLGRSDGICFNTLATLERSGEGAELSSLPLLDSKGYTWHEVTDQSLKGKILRRIKDRSFLPMHSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5USQ9</accession>
    <name>W5USQ9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11 13">Peroxisome proliferator-activated receptor gamma coactivator 1-beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Ppargc1b</name>
        <name evidence="13" type="synonym">ppargc1b</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT418264">
        <property type="protein sequence ID" value="AHH42673.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017329790.1">
        <property type="nucleotide sequence ID" value="XM_017474301.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5USQ9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000002211"/>
    <dbReference type="GeneID" id="108268932"/>
    <dbReference type="CTD" id="133522"/>
    <dbReference type="OMA" id="EPTKPCC"/>
    <dbReference type="OrthoDB" id="10047851at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034605">
        <property type="entry name" value="PGC-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15528">
        <property type="entry name" value="PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA COACTIVATOR 1 PGC-1 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15528:SF12">
        <property type="entry name" value="PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR GAMMA COACTIVATOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="724"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="115"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="219"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="306"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="395"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="452"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="633"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="772"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="123"/>
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        </location>
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            <begin position="219"/>
            <end position="235"/>
        </location>
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        <location>
            <begin position="236"/>
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        </location>
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            <end position="325"/>
        </location>
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        <location>
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            <end position="474"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="666"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AHH42673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017329790.1"/>
        </source>
    </evidence>
    <sequence length="834" mass="93193" checksum="4B9C27D6CAD65053" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5USW1</accession>
    <name>W5USW1_ICTPU</name>
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        <recommendedName>
            <fullName evidence="8">Protein canopy homolog 3</fullName>
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    </protein>
    <gene>
        <name evidence="12 15" type="primary">cnpy3</name>
        <name evidence="13" type="synonym">CNPY3</name>
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    <organism evidence="12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2019-02" db="EMBL/GenBank/DDBJ databases">
            <title>Analysis of Canopy family in channel catfish (Ictalurus punctatus) and waking catfish (Clarias batrachus) and their involvement in response to stimulation of Aeromonas hydrophila.</title>
            <authorList>
                <person name="Jiang X."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Heart</tissue>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the canopy family.</text>
    </comment>
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        <property type="protein sequence ID" value="AHH42728.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="MK501826">
        <property type="protein sequence ID" value="QEG99567.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017313431.1">
        <property type="nucleotide sequence ID" value="XM_017457942.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5USW1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013730"/>
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    <dbReference type="KEGG" id="ipu:108258921"/>
    <dbReference type="CTD" id="10695"/>
    <dbReference type="GeneTree" id="ENSGT00390000014072"/>
    <dbReference type="OMA" id="GQDKACL"/>
    <dbReference type="OrthoDB" id="6020060at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021852">
        <property type="entry name" value="DUF3456"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15382">
        <property type="entry name" value="CTG4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15382:SF2">
        <property type="entry name" value="PROTEIN CANOPY HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11938">
        <property type="entry name" value="DUF3456"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540376" description="Protein canopy homolog 3" evidence="10">
        <location>
            <begin position="17"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3456" evidence="11">
        <location>
            <begin position="31"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="201"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="219"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="238"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF11938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AHH42728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="QEG99567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017313431.1"/>
        </source>
    </evidence>
    <sequence length="272" mass="31572" checksum="6E1E11676C3891CC" modified="2014-04-16" version="1">MNVLIFIALIFVSTQAARKSDDDDWVHLPNKCEVCKFLSIEMKSALEETGKTKEVIETNYRFLDDKSAPPIKYVKSDIRFIEVMESVCSRILQYNLHKERDGSNRFAKGMSETFSTLHNLVHKGVKVVMDIPYELWNETSAEVSDLKKQCDVMVEQYEDVIEDWYKGSQEEDLMTYLCEKHVLKGQDTSCLKEIWAEKKGDPAAIAQDKKKKKSKKKAKGGEHNQNREKKVKKKKSKMHDAHSEEQKMEERGHTSDEEIQRSAPLHQERTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PK39</accession>
    <name>A0A2D0PK39_ICTPU</name>
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        <submittedName>
            <fullName evidence="11">Homeobox expressed in ES cells 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">hesx1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ANF homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017305776.1">
        <property type="nucleotide sequence ID" value="XM_017450287.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PK39"/>
    <dbReference type="GeneID" id="108254881"/>
    <dbReference type="KEGG" id="ipu:108254881"/>
    <dbReference type="CTD" id="8820"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021983">
        <property type="term" value="P:pituitary gland development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000214">
        <property type="entry name" value="Homeobox expressed in ES cells 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043402">
        <property type="entry name" value="Hesx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46966">
        <property type="entry name" value="HOMEOBOX EXPRESSED IN ES CELLS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46966:SF1">
        <property type="entry name" value="HOMEOBOX EXPRESSED IN ES CELLS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="9">
        <location>
            <begin position="80"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="82"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017305776.1"/>
        </source>
    </evidence>
    <sequence length="158" mass="18507" checksum="A83D95D602AD3D4D" modified="2017-12-20" version="1">MASVSAQQSRHSLFTIDRILGLDRPEQRTTMYTPHRPWTDVQPTKNVCFVKTDGSLDVPVMDERKSEAPVESCRRSLNWCIGRRPRTAFSSLQIEILESVFQVNSYPGIDVREELAQKLHLDEDRIQIWFQNRRAKLKRSHRENQFLMVKKVINGLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PKB7</accession>
    <name>A0A2D0PKB7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ubiquitin-conjugating enzyme E2 variant 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ube2v1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017306467.1">
        <property type="nucleotide sequence ID" value="XM_017450978.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PKB7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003446"/>
    <dbReference type="GeneID" id="108255212"/>
    <dbReference type="KEGG" id="ipu:108255212"/>
    <dbReference type="CTD" id="7335"/>
    <dbReference type="OMA" id="GPESCSY"/>
    <dbReference type="OrthoDB" id="6508832at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="CDD" id="cd23807">
        <property type="entry name" value="UEV_UBE2V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000012">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 variant 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="12"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017306467.1"/>
        </source>
    </evidence>
    <sequence length="147" mass="16621" checksum="025E62E26755FD5C" modified="2017-12-20" version="1">MAASTGSGIKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDDDMTLTRWRGMIIGPPRTIYENRIYSLRVECGPRYPETPPLVRFVTKINLNGVHNSNGVVDVRAVSSLAKWQNSYSIRVVLQELRRLMMCKENMKLPQPPEGQIYSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PM31</accession>
    <name>A0A2D0PM31_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dynein light chain roadblock</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">dynlrb1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
            <location evidence="1 10">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the GAMAD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017306476.1">
        <property type="nucleotide sequence ID" value="XM_017450987.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PM31"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020955"/>
    <dbReference type="GeneID" id="108255214"/>
    <dbReference type="KEGG" id="ipu:108255214"/>
    <dbReference type="CTD" id="83658"/>
    <dbReference type="OrthoDB" id="9985637at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.30:FF:000002">
        <property type="entry name" value="Dynein light chain roadblock"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016561">
        <property type="entry name" value="DYNLRB1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10779">
        <property type="entry name" value="DYNEIN LIGHT CHAIN ROADBLOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009998">
        <property type="entry name" value="DLC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103196">
        <property type="entry name" value="Roadblock/LC7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6 10" id="KW-0243">Dynein</keyword>
    <keyword evidence="5 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Roadblock/LAMTOR2" evidence="11">
        <location>
            <begin position="4"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR009998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017306476.1"/>
        </source>
    </evidence>
    <sequence length="96" mass="10900" checksum="A6F93F711CDBFCCC" modified="2017-12-20" version="1">MAEVEETLKRIQNQKGVQGIIIINSEGIPIKTTLDNSSTVQYAGLLHQLVMKARSTVRDIDAQNDLTFLRVRSKKNEIMIAPDKDYFLIVIQNPME</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PPG8</accession>
    <name>A0A2D0PPG8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">G-rich sequence factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">grsf1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017307326.1">
        <property type="nucleotide sequence ID" value="XM_017451837.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PPG8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000015463"/>
    <dbReference type="GeneID" id="108255698"/>
    <dbReference type="KEGG" id="ipu:108255698"/>
    <dbReference type="CTD" id="2926"/>
    <dbReference type="OMA" id="WSCTAQD"/>
    <dbReference type="OrthoDB" id="431068at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12503">
        <property type="entry name" value="RRM1_hnRNPH_GRSF1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12504">
        <property type="entry name" value="RRM2_hnRNPH_CRSF1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050666">
        <property type="entry name" value="ESRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13976">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="99"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="194"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017307326.1"/>
        </source>
    </evidence>
    <sequence length="378" mass="42712" checksum="D545F87954BE772A" modified="2017-12-20" version="1">MAASGRMALVSALVRHFSGGRSSCVRKLLETNQRLRVVSNVKFGLERDSHVWAVNPAKAPFSWDTQRRALSSETPYKEDDYPPLPEYPSTPESNQKEVFIVRVKGLPWSCSAEDLLKFFSECRIHKGVNGIHLMYHKNGKPTGQAFIELEDEEDVNKALEMHRQYLGPRYVEVYEVTNRDAEAILKGTDENQDGVVRIRGLPFNCAEEEVIQFFSGLNIVNNGVTLVMDRSGRSSGDAFVQFATQEMANEALKRDREVIGNRYIEVYPSKKSEIQAQYSRGRGDTTVTAVRAQKTSSVPLPTHYVHMRGLPYQATAGDIINFFHPIRVAKVLLEYGPDGRPNGEAGAHFTTRQDALQAMTKDKQYIQDRYIELYLNST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PPH4</accession>
    <name>A0A2D0PPH4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Protein phosphatase 1E</fullName>
            <ecNumber evidence="5">3.1.3.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Ca(2+)/calmodulin-dependent protein kinase phosphatase N</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">CaMKP-nucleus</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Partner of PIX 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Partner of PIX-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26 27 28" type="primary">ppm1f</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">SDA103</strain>
        </source>
    </reference>
    <reference evidence="26 27 28" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26 27 28">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="17">Heterotrimer. Interacts with PAX1 and ARHGEF6 (or ARHGEF7).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="23">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017307907.1">
        <property type="nucleotide sequence ID" value="XM_017452418.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047005757.1">
        <property type="nucleotide sequence ID" value="XM_047149801.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047005758.1">
        <property type="nucleotide sequence ID" value="XM_047149802.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PPH4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030123"/>
    <dbReference type="GeneID" id="108255987"/>
    <dbReference type="KEGG" id="ipu:108255987"/>
    <dbReference type="CTD" id="9647"/>
    <dbReference type="OMA" id="DEMFLFK"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000021">
        <property type="entry name" value="Protein phosphatase, Mg2+/Mn2+-dependent, 1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF233">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 23" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12 23" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="24">
        <location>
            <begin position="138"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00070214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00075580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00075701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00078590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00079435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_017307907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_047005757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_047005758.1"/>
        </source>
    </evidence>
    <sequence length="411" mass="45499" checksum="0865DFF9D3FFBD37" modified="2017-12-20" version="1">MNEQDGAQSFLESFVEEFANPLGQDDPLPLNPLSRTVTLEEVKGESLDLGLRLLSSRKAPGWLSASMCHAAVTELLKDDLSPFHCPKDPEQQQETEAEVVLLQSEPVQRLFINKLQEVGVAWQKQLPNRTPRSSRSRLCSVHAIRNTRRKMEDRHVLLPEFNQLFGLQDGVEREYYAVFDGHGGVDAATYSSTHLHVILSQQAVLKSDPSTAFKKSFIQTDDMFRIKARRERMRSGSTGVAVLLAADWLTVSWLGDSQAMLVRKGEPVILMDPHKPEREDEKKRIEDLGGCIAFMGCWRVNGTYAVSRAIGDFDQKPYVSNAADCSSVQLNGDEDYVLLACDGFFDVLRPADVPALVLEALRETGGSGEDVAQSLVAQAKAAGSSDNITVLLVFLREPRDLLLSEITAGQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PW70</accession>
    <name>A0A2D0PW70_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein si:dkeyp-97a10.3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">si:dkeyp-97a10.3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017309375.2">
        <property type="nucleotide sequence ID" value="XM_017453886.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PW70"/>
    <dbReference type="GeneID" id="108256724"/>
    <dbReference type="KEGG" id="ipu:108256724"/>
    <dbReference type="OrthoDB" id="5969816at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052598">
        <property type="entry name" value="IgSF_CEA-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44337">
        <property type="entry name" value="CARCINOEMBRYONIC ANTIGEN-RELATED CELL ADHESION MOLECULE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44337:SF22">
        <property type="entry name" value="HEPACAM FAMILY MEMBER 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039929159" evidence="7">
        <location>
            <begin position="22"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="24"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="145"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="224"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="367"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="510"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="557"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="706"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="911"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="420"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="557"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="568"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="616"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="658"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017309375.2"/>
        </source>
    </evidence>
    <sequence length="939" mass="102236" checksum="8457AA9D73FB2954" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PYC2</accession>
    <name>A0A2D0PYC2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Kinesin-like protein KIF25</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">kif25</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017311032.1">
        <property type="nucleotide sequence ID" value="XM_017455543.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PYC2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034205"/>
    <dbReference type="GeneID" id="108257626"/>
    <dbReference type="KEGG" id="ipu:108257626"/>
    <dbReference type="CTD" id="3834"/>
    <dbReference type="OMA" id="CIGMSGV"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972:SF63">
        <property type="entry name" value="KINESIN FAMILY MEMBER 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KLP-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="8">
        <location>
            <begin position="174"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="431"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="439"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="268"/>
            <end position="275"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017311032.1"/>
        </source>
    </evidence>
    <sequence length="595" mass="66304" checksum="815744F6ACE5417F" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q132</accession>
    <name>A0A2D0Q132_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">E3 ubiquitin/ISG15 ligase TRIM25</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC108257667</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017311080.1">
        <property type="nucleotide sequence ID" value="XM_017455591.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q132"/>
    <dbReference type="GeneID" id="108257667"/>
    <dbReference type="KEGG" id="ipu:108257667"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16040">
        <property type="entry name" value="SPRY_PRY_SNTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF5">
        <property type="entry name" value="E3 UBIQUITIN_ISG15 LIGASE TRIM25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017311080.1"/>
        </source>
    </evidence>
    <sequence length="542" mass="62175" checksum="34213EC112917870" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q3X0</accession>
    <name>A0A2D0Q3X0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13 14 15">ETS-related transcription factor Elf-1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13 14 15 16" type="primary">LOC108258693</name>
    </gene>
    <organism evidence="12 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13 14 15" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13 14 15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017312990.1">
        <property type="nucleotide sequence ID" value="XM_017457501.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017312991.1">
        <property type="nucleotide sequence ID" value="XM_017457502.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017312992.1">
        <property type="nucleotide sequence ID" value="XM_017457503.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053532061.1">
        <property type="nucleotide sequence ID" value="XM_053676086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q3X0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018867"/>
    <dbReference type="GeneID" id="108258693"/>
    <dbReference type="KEGG" id="ipu:108258693"/>
    <dbReference type="OrthoDB" id="8196042at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000853">
        <property type="entry name" value="ETS-Like transcription Factor homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022084">
        <property type="entry name" value="TF_Elf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF191">
        <property type="entry name" value="ECDYSONE-INDUCED PROTEIN 74EF ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12310">
        <property type="entry name" value="Elf-1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ETS" evidence="11">
        <location>
            <begin position="183"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="141"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
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        <source>
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        <source>
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        </source>
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        </source>
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    <sequence length="595" mass="65195" checksum="A78181F63742B047" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q4J3</accession>
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        <submittedName>
            <fullName evidence="9">Sterile alpha motif domain-containing protein 1</fullName>
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        <name evidence="9" type="primary">samd1b</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
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                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="9">Blood</tissue>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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        <source>
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</entry>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
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                <person name="Kirby M.L."/>
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                <person name="Flanagan M.E."/>
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                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="8">Blood</tissue>
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        <location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="5">
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017313644.1"/>
        </source>
    </evidence>
    <sequence length="146" mass="16294" checksum="E57E7D336395F7B1" modified="2017-12-20" version="1">MGNCLSSQSADDLSLLNESEGASLPGEPPPPYQERVQVPVYHPTPSQARLATQLTEEEQIRIAQRIGLIQHLPRGIFNPGSEPSDKKIKECVICMLDFEYGDPIRFLPCMHIYHVACIDAWLMRSFTCPSCMEPVDAALLSSYETN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q7C0</accession>
    <name>A0A2D0Q7C0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein LOC108259526</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC108259526</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017314593.2">
        <property type="nucleotide sequence ID" value="XM_017459104.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q7C0"/>
    <dbReference type="GeneID" id="108259526"/>
    <dbReference type="KEGG" id="ipu:108259526"/>
    <dbReference type="OrthoDB" id="8939957at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5037940656" evidence="2">
        <location>
            <begin position="19"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="3">
        <location>
            <begin position="19"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017314593.2"/>
        </source>
    </evidence>
    <sequence length="215" mass="23965" checksum="0297EEBFFD1863BD" modified="2023-02-22" version="2">MTTVLWFYLLVCCGAAEAFTEKSVDLGQNVTLKCQVGVRDVFWFLIKPSESLVIILRSYSIMALEPIYSNMTFSKIYSVQYNSSLFIHNISTNELGVYYCLRTGSPPDIGSGIRLYIRNHSADNRTGSKEAVLLPILLISVIMNFVLAVPVAVRHCRRRPKTRAQPSNPGLQPRQDSTDHVYAEVQFSTTNGPVRTANGPVRTANLDSTYALVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q8B1</accession>
    <name>A0A2D0Q8B1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Sodium-independent sulfate anion transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Solute carrier family 26 member 11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22 23 24 25 26" type="primary">slc26a11</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22 23 24" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22 23 24">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Sodium-independent anion exchanger mediating bicarbonate, chloride, sulfate and oxalate transport. Exhibits sodium-independent sulfate anion transporter activity that may cooperate with SLC26A2 to mediate DIDS-sensitive sulfate uptake into high endothelial venules endothelial cells (HEVEC). In the kidney, mediates chloride-bicarbonate exchange, facilitating V-ATPase-mediated acid secretion. May function as a chloride channel, playing an important role in moderating chloride homeostasis and neuronal activity in the cerebellum.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>hydrogencarbonate(in) + chloride(out) = hydrogencarbonate(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:72363"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>oxalate(in) + chloride(out) = oxalate(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:72263"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>sulfate(in) + H(+)(in) = sulfate(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28574"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Basolateral cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017314537.1">
        <property type="nucleotide sequence ID" value="XM_017459048.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017314538.1">
        <property type="nucleotide sequence ID" value="XM_017459049.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047007544.1">
        <property type="nucleotide sequence ID" value="XM_047151588.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047007545.1">
        <property type="nucleotide sequence ID" value="XM_047151589.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047007546.1">
        <property type="nucleotide sequence ID" value="XM_047151590.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q8B1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013865"/>
    <dbReference type="GeneID" id="108259501"/>
    <dbReference type="KEGG" id="ipu:108259501"/>
    <dbReference type="CTD" id="284129"/>
    <dbReference type="OrthoDB" id="288203at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008509">
        <property type="term" value="F:monoatomic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008271">
        <property type="term" value="F:secondary active sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.24:FF:000013">
        <property type="entry name" value="Solute carrier family 26 member 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018045">
        <property type="entry name" value="S04_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01130">
        <property type="entry name" value="SLC26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0039">Anion exchange</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="93"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="335"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="373"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="400"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="438"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="20">
        <location>
            <begin position="473"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00054369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017314537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017314538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_047007544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_047007545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_047007546.1"/>
        </source>
    </evidence>
    <sequence length="581" mass="62450" checksum="AE2F43CFA21C2D76" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q8H6</accession>
    <name>A0A2D0Q8H6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DDB1- and CUL4-associated factor 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">dcaf15</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017313665.1">
        <property type="nucleotide sequence ID" value="XM_017458176.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q8H6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008800"/>
    <dbReference type="GeneID" id="108259042"/>
    <dbReference type="KEGG" id="ipu:108259042"/>
    <dbReference type="CTD" id="90379"/>
    <dbReference type="OMA" id="QILYTKG"/>
    <dbReference type="OrthoDB" id="6354267at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20913">
        <property type="entry name" value="DCAF15-CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20917">
        <property type="entry name" value="DCAF15-NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038914">
        <property type="entry name" value="DCAF15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047319">
        <property type="entry name" value="DCAF15_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032734">
        <property type="entry name" value="DCAF15_WD40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28541">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28541:SF1">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14939">
        <property type="entry name" value="DCAF15_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDB1- and CUL4-associated factor 15 WD40 repeat-containing" evidence="2">
        <location>
            <begin position="39"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="398"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017313665.1"/>
        </source>
    </evidence>
    <sequence length="633" mass="70644" checksum="8513D47826B41636" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QFH1</accession>
    <name>A0A2D0QFH1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Carotenoid-cleaving dioxygenase, mitochondrial</fullName>
            <ecNumber evidence="11">1.13.11.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">LOC108260375</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">SDA103</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="27">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Broad specificity mitochondrial dioxygenase that mediates the asymmetric oxidative cleavage of carotenoids. Cleaves carotenes (pure hydrocarbon carotenoids) such as all-trans-beta-carotene and lycopene as well as xanthophylls (oxygenated carotenoids) such as zeaxanthin, lutein and beta-cryptoxanthin at both the 9,10 and the 9',10' carbon-carbon double bond. Through its function in carotenoids metabolism regulates oxidative stress and the production of important signaling molecules.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(3R)-3-hydroxy-10'-apo-beta-carotenal + O2 = 4,9-dimethyldodeca-2,4,6,8,10-pentaenedial + (3R)-hydroxy-beta-ionone</text>
            <dbReference type="Rhea" id="RHEA:68424"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:53171"/>
            <dbReference type="ChEBI" id="CHEBI:53173"/>
            <dbReference type="ChEBI" id="CHEBI:177902"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:68425"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(3R,6R)-3-hydroxy-10'-apo-alpha-carotenal + O2 = (3R,6R)-hydroxy-alpha-ionone + 4,9-dimethyldodeca-2,4,6,8,10-pentaenedial</text>
            <dbReference type="Rhea" id="RHEA:68436"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:53171"/>
            <dbReference type="ChEBI" id="CHEBI:177903"/>
            <dbReference type="ChEBI" id="CHEBI:177904"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:68437"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>13-cis-lycopene + O2 = 13-cis-10'-apo-lycopenal + (3E,5E)-6,10-dimethylundeca-3,5,9-trien-2-one</text>
            <dbReference type="Rhea" id="RHEA:68448"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:67207"/>
            <dbReference type="ChEBI" id="CHEBI:177907"/>
            <dbReference type="ChEBI" id="CHEBI:177908"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:68449"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>5-cis-lycopene + O2 = 5-cis-10'-apo-lycopenal + (3E,5E)-6,10-dimethylundeca-3,5,9-trien-2-one</text>
            <dbReference type="Rhea" id="RHEA:68444"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:67207"/>
            <dbReference type="ChEBI" id="CHEBI:177905"/>
            <dbReference type="ChEBI" id="CHEBI:177906"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:68445"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>all-trans-10'-apo-beta-carotenal + O2 = beta-ionone + 4,9-dimethyldodeca-2,4,6,8,10-pentaenedial</text>
            <dbReference type="Rhea" id="RHEA:68452"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:32325"/>
            <dbReference type="ChEBI" id="CHEBI:53153"/>
            <dbReference type="ChEBI" id="CHEBI:53171"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:68453"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>all-trans-beta-carotene + O2 = beta-ionone + all-trans-10'-apo-beta-carotenal</text>
            <dbReference type="Rhea" id="RHEA:26389"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17579"/>
            <dbReference type="ChEBI" id="CHEBI:32325"/>
            <dbReference type="ChEBI" id="CHEBI:53153"/>
            <dbReference type="EC" id="1.13.11.71"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:26390"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>all-trans-zeaxanthin + 2 O2 = 4,9-dimethyldodeca-2,4,6,8,10-pentaenedial + 2 (3R)-hydroxy-beta-ionone</text>
            <dbReference type="Rhea" id="RHEA:26393"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:27547"/>
            <dbReference type="ChEBI" id="CHEBI:53171"/>
            <dbReference type="ChEBI" id="CHEBI:53173"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:26394"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>all-trans-zeaxanthin + O2 = (3R)-3-hydroxy-10'-apo-beta-carotenal + (3R)-hydroxy-beta-ionone</text>
            <dbReference type="Rhea" id="RHEA:68104"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:27547"/>
            <dbReference type="ChEBI" id="CHEBI:53173"/>
            <dbReference type="ChEBI" id="CHEBI:177902"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:68105"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>beta-cryptoxanthin + O2 = all-trans-10'-apo-beta-carotenal + (3R)-hydroxy-beta-ionone</text>
            <dbReference type="Rhea" id="RHEA:68440"/>
            <dbReference type="ChEBI" id="CHEBI:10362"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:53153"/>
            <dbReference type="ChEBI" id="CHEBI:53173"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:68441"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>lutein + O2 = (3R,6R)-3-hydroxy-10'-apo-alpha-carotenal + (3R)-hydroxy-beta-ionone</text>
            <dbReference type="Rhea" id="RHEA:68432"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:28838"/>
            <dbReference type="ChEBI" id="CHEBI:53173"/>
            <dbReference type="ChEBI" id="CHEBI:177903"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:68433"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>lutein + O2 = (3R,6R)-hydroxy-alpha-ionone + (3R)-3-hydroxy-10'-apo-beta-carotenal</text>
            <dbReference type="Rhea" id="RHEA:68428"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:28838"/>
            <dbReference type="ChEBI" id="CHEBI:177902"/>
            <dbReference type="ChEBI" id="CHEBI:177904"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:68429"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="23">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="23">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 24">Belongs to the carotenoid oxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.71" evidence="11"/>
    <dbReference type="RefSeq" id="XP_017316115.1">
        <property type="nucleotide sequence ID" value="XM_017460626.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QFH1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013950"/>
    <dbReference type="GeneID" id="108260375"/>
    <dbReference type="KEGG" id="ipu:108260375"/>
    <dbReference type="OMA" id="WHIGDYN"/>
    <dbReference type="OrthoDB" id="407010at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102076">
        <property type="term" value="F:beta,beta-carotene-9',10'-cleaving oxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003834">
        <property type="term" value="F:beta-carotene 15,15'-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010436">
        <property type="term" value="F:carotenoid dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016121">
        <property type="term" value="P:carotene catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042574">
        <property type="term" value="P:retinal metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004294">
        <property type="entry name" value="Carotenoid_Oase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543">
        <property type="entry name" value="BETA-CAROTENE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543:SF122">
        <property type="entry name" value="CAROTENOID-CLEAVING DIOXYGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03055">
        <property type="entry name" value="RPE65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 27" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6 23" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3 23" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="543"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00035797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00049207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR604294-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU003799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_017316115.1"/>
        </source>
    </evidence>
    <sequence length="550" mass="62635" checksum="98A1B0DDB75DA366" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QJR3</accession>
    <name>A0A2D0QJR3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Sulfotransferase</fullName>
            <ecNumber evidence="13">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17 18" type="primary">chst3a</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17 18">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>3'-phosphoadenylyl sulfate + keratan = adenosine 3',5'-bisphosphate + keratan 6'-sulfate.</text>
            <dbReference type="EC" id="2.8.2.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>chondroitin beta-D-glucuronate + n 3'-phosphoadenylyl sulfate = chondroitin 6'-sulfate + n adenosine 3',5'-bisphosphate + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:11108"/>
            <dbReference type="Rhea" id="RHEA-COMP:9827"/>
            <dbReference type="Rhea" id="RHEA-COMP:9828"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57652"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:62065"/>
            <dbReference type="EC" id="2.8.2.17"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:11109"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="13"/>
    <dbReference type="RefSeq" id="XP_017318547.1">
        <property type="nucleotide sequence ID" value="XM_017463058.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017318548.1">
        <property type="nucleotide sequence ID" value="XM_017463059.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QJR3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000036743"/>
    <dbReference type="GeneID" id="108262760"/>
    <dbReference type="KEGG" id="ipu:108262760"/>
    <dbReference type="CTD" id="559721"/>
    <dbReference type="GeneTree" id="ENSGT00940000161045"/>
    <dbReference type="OrthoDB" id="6138663at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008459">
        <property type="term" value="F:chondroitin 6-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045130">
        <property type="term" value="F:keratan sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001517">
        <property type="term" value="F:N-acetylglucosamine 6-O-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006790">
        <property type="term" value="P:sulfur compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000938">
        <property type="entry name" value="Sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016469">
        <property type="entry name" value="Carbohydrate_sulfotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051135">
        <property type="entry name" value="Gal/GlcNAc/GalNAc_ST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10704">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10704:SF73">
        <property type="entry name" value="SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005883">
        <property type="entry name" value="Carbohydrate_sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="22"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="15">
        <location>
            <begin position="92"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017318547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017318548.1"/>
        </source>
    </evidence>
    <sequence length="444" mass="51365" checksum="ED60FC8FAF3C69EE" modified="2017-12-20" version="1">MNLNSYTYRLWRDFQTETYSRMRNKYVILIICIVALVIIEKENNIISRVSEKLTHKQTQQTPLSLFNGSSLDDSEMSVNSTQLVDTDQAGHKHILLLATTRTGSSFVGEFFNQLGTNMFYLFEPLWHVERTLSQDTGIPNSNISSIAYRDVLLSLFLCNFSLLESFINPPPLEHVTPALFRRESSMALCEEQVCTPVQKKVFERYRCRTRRCGPLNFTLASQSCLRKKHRAIKTVRIRQLDSLRLLVEDPRLDIKVIQLVRDPRAVLASRMVAFSSKYQTWKSWAANGEVPTDDEEVKRLQGNCNQIRESAELGLSQPDWLENRYMLVRYEDIARYPMQKAAEMYRFTGIPFTAQAREWIIKNTHAAEVASGVYSTQKNSSEQVEKWRFSIPFKLAQAVQEVCGPTMELFGYRFVDSEETLVNRSISLLEEKQFNVSYVTDYNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QNI3</accession>
    <name>A0A2D0QNI3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Angiotensinogen</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Serpin A8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">agt</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone. Acts by binding to angiotensin receptors AGTR1 and AGTR2. Also binds the DEAR/FBXW7-AS1 receptor.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Is a ligand for the G-protein coupled receptor MAS1. Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Stimulates aldosterone release.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017319957.1">
        <property type="nucleotide sequence ID" value="XM_017464468.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QNI3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022235"/>
    <dbReference type="GeneID" id="108263554"/>
    <dbReference type="KEGG" id="ipu:108263554"/>
    <dbReference type="CTD" id="183"/>
    <dbReference type="OMA" id="FVPMMTV"/>
    <dbReference type="OrthoDB" id="7817921at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003081">
        <property type="term" value="P:regulation of systemic arterial blood pressure by renin-angiotensin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042310">
        <property type="term" value="P:vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000227">
        <property type="entry name" value="Angiotensinogen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF13">
        <property type="entry name" value="ANGIOTENSINOGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00654">
        <property type="entry name" value="ANGIOTENSNGN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 16" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0838">Vasoactive</keyword>
    <keyword evidence="10" id="KW-0839">Vasoconstrictor</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012452070" description="Angiotensinogen" evidence="16">
        <location>
            <begin position="17"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Serpin" evidence="17">
        <location>
            <begin position="88"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017319957.1"/>
        </source>
    </evidence>
    <sequence length="451" mass="51072" checksum="33D59646B1B67398" modified="2017-12-20" version="1">MKLFLLMLTCFAMGLANRVYIHPFSLLDSGDVTCEFTNSKDKGPLETIRSLTPIQDGTEPDPRPTIGELKNLTRWTAVVAELQNSQGLRMYQALSRKQEKVNVLFSPYASFATLLTLYLGTSKSTANDYQQFLGLIWNTEKPGCVKAINGHKVLRALNAISSVIDGPQDELRTRVWTFVSSDADLSKDFVRGIQEFTDTSFIRAVNFSQPKEAEAQVNLFIQKTSHSKVENLFKDISQSTNLLFATSVHFKGNWRTAFQPEKTTVQDFQIDDMSTVKVPLMTHTGDYMHLNDPDKKCTVVKLGLSKRTYMLLVLPSEKARLQDIENQLQADTISTWHKHLKEQYLELSLPKFSLTAVTDLRSLLSDMALDKLLLGSEANFQRFSTKGNFTIDKVINKVLFEMSEERKEVPNKSQDDRVPLRVTVDRPFLFTIVEGNTNAILMLGRILNPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QRE6</accession>
    <name>A0A2D0QRE6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serum response factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">srfb</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017320055.1">
        <property type="nucleotide sequence ID" value="XM_017464566.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QRE6"/>
    <dbReference type="GeneID" id="108263590"/>
    <dbReference type="KEGG" id="ipu:108263590"/>
    <dbReference type="CTD" id="393604"/>
    <dbReference type="OrthoDB" id="2284405at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010736">
        <property type="term" value="F:serum response element binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060379">
        <property type="term" value="P:cardiac muscle cell myoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002042">
        <property type="term" value="P:cell migration involved in sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902895">
        <property type="term" value="P:positive regulation of miRNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042060">
        <property type="term" value="P:wound healing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00266">
        <property type="entry name" value="MADS_SRF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000002">
        <property type="entry name" value="Serum response factor b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033897">
        <property type="entry name" value="SRF-like_MADS-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="8">
        <location>
            <begin position="187"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="62"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="122"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="264"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="464"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="292"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="465"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00072990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017320055.1"/>
        </source>
    </evidence>
    <sequence length="612" mass="61635" checksum="375859305301C34B" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QRU7</accession>
    <name>A0A2D0QRU7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">WAP four-disulfide core domain protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">wfdc1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Has growth inhibitory activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017321132.1">
        <property type="nucleotide sequence ID" value="XM_017465643.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QRU7"/>
    <dbReference type="GeneID" id="108264276"/>
    <dbReference type="KEGG" id="ipu:108264276"/>
    <dbReference type="CTD" id="58189"/>
    <dbReference type="OMA" id="RFYKEYP"/>
    <dbReference type="OrthoDB" id="5989673at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001558">
        <property type="term" value="P:regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.75.10:FF:000003">
        <property type="entry name" value="WAP four-disulfide core domain protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.75.10">
        <property type="entry name" value="Elafin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036645">
        <property type="entry name" value="Elafin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008197">
        <property type="entry name" value="WAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042357">
        <property type="entry name" value="WFDC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14308">
        <property type="entry name" value="WAP FOUR-DISULFIDE CORE DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14308:SF0">
        <property type="entry name" value="WAP FOUR-DISULFIDE CORE DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00095">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00217">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57256">
        <property type="entry name" value="Elafin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51390">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043377417" description="WAP four-disulfide core domain protein 1" evidence="10">
        <location>
            <begin position="26"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="WAP" evidence="11">
        <location>
            <begin position="49"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="30"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017321132.1"/>
        </source>
    </evidence>
    <sequence length="212" mass="23900" checksum="733D9084A5C1843E" modified="2017-12-20" version="1">MAGAPIPSRFVLIVCLLLLVTSTESKRRLRRRGLSQKDYEYPNNSQSTQHQKNDRCPPPPQMLPERACEVPGCRSDSECERHKRCCYNGCIYTCLESVQPPPVLDWLVQPKPRWLGGNGWLLDGPEEVLQAEACSTTEDGDEPLHCPTGYECHIINPGNPSVGIPNRGQCIKQRGNSDGRSLRHKYFKDHKDYLGSNSNIAVGYEKYHKHLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QS56</accession>
    <name>A0A2D0QS56_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Formin-like protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">fmnl1b</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6 7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin homology family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017321293.1">
        <property type="nucleotide sequence ID" value="XM_017465804.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017321303.1">
        <property type="nucleotide sequence ID" value="XM_017465814.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QS56"/>
    <dbReference type="GeneID" id="108264360"/>
    <dbReference type="KEGG" id="ipu:108264360"/>
    <dbReference type="CTD" id="100536330"/>
    <dbReference type="OrthoDB" id="1668162at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2220:FF:000001">
        <property type="entry name" value="Formin-like 1, isoform CRA_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000045">
        <property type="entry name" value="Formin-like protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010472">
        <property type="entry name" value="FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043592">
        <property type="entry name" value="FMNL_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010473">
        <property type="entry name" value="GTPase-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45857">
        <property type="entry name" value="FORMIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45857:SF2">
        <property type="entry name" value="FORMIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06367">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06371">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01139">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01140">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="3">
        <location>
            <begin position="36"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="4">
        <location>
            <begin position="573"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="447"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="534"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="181"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="455"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="541"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017321293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017321303.1"/>
        </source>
    </evidence>
    <sequence length="1044" mass="118700" checksum="0C20345F4EF18A73" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QTF2</accession>
    <name>A0A2D0QTF2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dynein regulatory complex subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Growth arrest-specific protein 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC108264575</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
            <location evidence="1">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DRC4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017321708.1">
        <property type="nucleotide sequence ID" value="XM_017466219.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QTF2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009710"/>
    <dbReference type="GeneID" id="108264575"/>
    <dbReference type="KEGG" id="ipu:108264575"/>
    <dbReference type="OrthoDB" id="767661at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030317">
        <property type="term" value="P:flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039308">
        <property type="entry name" value="GAS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025593">
        <property type="entry name" value="GAS8_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31543">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31543:SF0">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13851">
        <property type="entry name" value="GAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0969">Cilium</keyword>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Growth arrest-specific protein 8" evidence="15">
        <location>
            <begin position="247"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="200"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="292"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="19"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017321708.1"/>
        </source>
    </evidence>
    <sequence length="402" mass="47531" checksum="194A972239C7246F" modified="2017-12-20" version="1">MPPKKKGTGKSAKEKSSTVDQVPSTTEENGSTVAQGPSTAEEKSITVDQGPSKEQLEKQIIQMREELDREQKERNYFQQERDKIHDFWEISKSHLEEKYAEIRNRDKELEESAEKHQREIRKYKEKIKDMLLEHQNSIAECKHEGVVATKLLEKERADLESELQRDIHRLKVDSKEQELSYEKSIKSLKLSHDKEITKLRSEFEEKLREINATYEKKLEEQQEELDLRRKTEIHEIEKQKHAHIKKLIKSHDKAFSNIKNYYKDITYESVNQIDSLEEKVADKNSKMLEKEMPKVIQQNKRLTELLQKATQEVSELQKQLTDYQKDKSILAGKQACLKVADAELKNLIFEHELLEQRFRKVQQDRDELYKKFDKAILEVQQKRGFKNLLQEKKLVAQTETGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QTH9</accession>
    <name>A0A2D0QTH9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 16 17">Diphosphomevalonate decarboxylase</fullName>
            <ecNumber evidence="4 16 17">4.1.1.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">mvda</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 17">Catalyzes the ATP dependent decarboxylation of (R)-5-diphosphomevalonate to form isopentenyl diphosphate (IPP). Functions in the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoids and sterol synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>(R)-5-diphosphomevalonate + ATP = isopentenyl diphosphate + ADP + phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:23732"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57557"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.1.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 17">Steroid biosynthesis; cholesterol biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 16 17">Belongs to the diphosphomevalonate decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.33" evidence="4 16 17"/>
    <dbReference type="RefSeq" id="XP_017321722.1">
        <property type="nucleotide sequence ID" value="XM_017466233.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QTH9"/>
    <dbReference type="GeneID" id="108264584"/>
    <dbReference type="CTD" id="492781"/>
    <dbReference type="OrthoDB" id="10253702at2759"/>
    <dbReference type="UniPathway" id="UPA00063"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004163">
        <property type="term" value="F:diphosphomevalonate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006695">
        <property type="term" value="P:cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000018">
        <property type="entry name" value="Diphosphomevalonate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.890:FF:000005">
        <property type="entry name" value="Diphosphomevalonate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005935">
        <property type="entry name" value="Mev_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029765">
        <property type="entry name" value="Mev_diP_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053859">
        <property type="entry name" value="MVD-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041431">
        <property type="entry name" value="Mvd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01240">
        <property type="entry name" value="mevDPdecarb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10977">
        <property type="entry name" value="DIPHOSPHOMEVALONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10977:SF3">
        <property type="entry name" value="DIPHOSPHOMEVALONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18376">
        <property type="entry name" value="MDD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22700">
        <property type="entry name" value="MVD-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015950">
        <property type="entry name" value="Mev_P_decrbx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0152">Cholesterol biosynthesis</keyword>
    <keyword evidence="17" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="6 17" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11 16 17" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14 16 17" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 17" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="13 17" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="10 17" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="12 17" id="KW-1207">Sterol metabolism</keyword>
    <feature type="domain" description="Diphosphomevalonate decarboxylase-like N-terminal" evidence="19">
        <location>
            <begin position="16"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Mvd1 C-terminal" evidence="18">
        <location>
            <begin position="188"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR015950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU363086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF18376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF22700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017321722.1"/>
        </source>
    </evidence>
    <sequence length="391" mass="43339" checksum="34DE1D644A818C77" modified="2017-12-20" version="1">MSVCSDSYSQVTAEVGGKRDEELILPINSSLSVTLHQDQLKTTTTVACSRNFQEDRIWLNGKEEDINQPRLQSCLREIRRLARKRRNEEDPNAEITLSHRVHICSVNNFPTAAGLASSAAGYACLVYSLSRLFGVDAELSLVARQGSGSACRSMYGGFVQWKMGERQDGKDSVAEQVAPETHWPELRVLILVVSAERKPVGSTSGMQTSVQTSKLLKHRADSVVPARMDDMIRAVRGRDFATFAELTMKDSNQFHATCLDTFPPIFYLNDISRKVINLVHRYNRHYRETRVAYTFDAGPNAVIYLLRDHVPEFVQAVRHFFPPESNGGEFVKGLPVHSASLPDELVRDIGMEPTPKGIGYIISTKVGPGPCAVDDPTLHLLGADGLPKKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QTL6</accession>
    <name>A0A2D0QTL6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RING finger protein 141</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">rnf141</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8 9">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017321772.1">
        <property type="nucleotide sequence ID" value="XM_017466283.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017321773.1">
        <property type="nucleotide sequence ID" value="XM_017466284.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QTL6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027330"/>
    <dbReference type="GeneID" id="108264600"/>
    <dbReference type="KEGG" id="ipu:108264600"/>
    <dbReference type="CTD" id="50862"/>
    <dbReference type="GeneTree" id="ENSGT00390000003145"/>
    <dbReference type="OMA" id="RHRNCPV"/>
    <dbReference type="OrthoDB" id="1630758at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16545">
        <property type="entry name" value="RING-HC_RNF141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043400">
        <property type="entry name" value="RING-HC_RNF141"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109:SF3">
        <property type="entry name" value="RING FINGER PROTEIN 141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="153"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017321772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017321773.1"/>
        </source>
    </evidence>
    <sequence length="226" mass="24846" checksum="21DD22DF8110F37F" modified="2017-12-20" version="1">MGQQISGQAAMGKLPEKLLKHAGLVRDSSYLTYEEFLAHIAELNEVTAKLAIGQPKHLLFEVQPGSDASALWKVAVRIVCTKINKEDGMVEASRIMNLYQFIQLYKDITSQAAEVLAAEGSMECSSGQLFSAGSCQASMWMGRVKQLTDEEECCICMDGKADLILSCAHSFCQKCIDKWSGRNCPICRIQLTAANEPWVMSDAPTEEDIASYILNLADEAGHPHRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QU88</accession>
    <name>A0A2D0QU88_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhombotin-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">LIM domain only protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">lmo2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017321993.1">
        <property type="nucleotide sequence ID" value="XM_017466504.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QU88"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000234"/>
    <dbReference type="GeneID" id="108264719"/>
    <dbReference type="KEGG" id="ipu:108264719"/>
    <dbReference type="CTD" id="4005"/>
    <dbReference type="OMA" id="MCGGCQQ"/>
    <dbReference type="OrthoDB" id="6352355at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09384">
        <property type="entry name" value="LIM1_LMO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000016">
        <property type="entry name" value="LIM domain only 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050945">
        <property type="entry name" value="LMO_RBTN_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45787">
        <property type="entry name" value="LD11652P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45787:SF3">
        <property type="entry name" value="RHOMBOTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="28"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="92"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017321993.1"/>
        </source>
    </evidence>
    <sequence length="159" mass="18662" checksum="530915E813DE36D6" modified="2017-12-20" version="1">MASTIERKTMEVNEELVDEVLQVPPSMLMCGGCQQSIGDRFFLKAIEQYWHEDCLSCDLCGCRLGEVGRRLYYKLGRKLCRRDYLRLFGQDGLCASCEKRIRAFEMTMRVRDKVYHLECFKCAACQKHFCVGDRYLLINTDIVCEQDIFEWTKLNSNLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QUX0</accession>
    <name>A0A2D0QUX0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Selenoprotein O</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">selenoo1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SELO family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017322217.1">
        <property type="nucleotide sequence ID" value="XM_017466728.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000024031"/>
    <dbReference type="GeneID" id="108264825"/>
    <dbReference type="KEGG" id="ipu:108264825"/>
    <dbReference type="CTD" id="558648"/>
    <dbReference type="OrthoDB" id="10254721at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00692">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003846">
        <property type="entry name" value="SelO"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000658">
        <property type="entry name" value="PRK00029.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12153:SF15">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE SELO, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12153">
        <property type="entry name" value="SELENOPROTEIN O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02696">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0711">Selenium</keyword>
    <keyword evidence="13" id="KW-0712">Selenocysteine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="641"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="526"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="646"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="13">
        <location>
            <position position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017322217.1"/>
        </source>
    </evidence>
    <sequence length="689" mass="77677" checksum="B9B408142DE112EF" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QX44</accession>
    <name>A0A2D0QX44_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transcriptional repressor scratch 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Scratch homolog 1 zinc finger protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">scrt2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="11">Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the snail C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017322884.1">
        <property type="nucleotide sequence ID" value="XM_017467395.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QX44"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008370"/>
    <dbReference type="GeneID" id="108265254"/>
    <dbReference type="KEGG" id="ipu:108265254"/>
    <dbReference type="CTD" id="85508"/>
    <dbReference type="OMA" id="GVPRHTC"/>
    <dbReference type="OrthoDB" id="5428132at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000560">
        <property type="entry name" value="Scratch family transcriptional repressor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000043">
        <property type="entry name" value="Scratch family zinc finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000169">
        <property type="entry name" value="transcriptional repressor scratch 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000207">
        <property type="entry name" value="zinc finger protein SNAI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050527">
        <property type="entry name" value="Snail/Krueppel_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388:SF98">
        <property type="entry name" value="SCRATCH-A TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="162"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="193"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="219"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="247"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="275"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="90"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017322884.1"/>
        </source>
    </evidence>
    <sequence length="312" mass="34789" checksum="068CDB9EF53FD8D5" modified="2017-12-20" version="1">MPRSFLVKKIKLDDFSSSPVAASNHHHHHHHHHLDESFTFTRSITDSVTSLGIRLSENGYIQDYISPSAYQGEKKLSSAVSDPLYAPVNSGGEEYCEPDLEHPDSPQSGLTARGYYSGESESQSDGYTADGFFISDGRSRRRGEAKEARKGGVEVKESGARHTCNECGKTYATSSNLSRHKQTHRSLDSKMARKCPTCGKVYVSMPALAMHVLTHDLKHKCNVCSKAFSRPWLLQGHMRSHTGEKPFACAHCGKAFADRSNLRAHMQTHSAFKHYRCKRCHKTFALKSYLNKHYESACFKGSGDEDESGSEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R2M9</accession>
    <name>A0A2D0R2M9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">2-oxoglutarate receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC108266248</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017324824.1">
        <property type="nucleotide sequence ID" value="XM_017469335.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R2M9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000001372"/>
    <dbReference type="GeneID" id="108266248"/>
    <dbReference type="KEGG" id="ipu:108266248"/>
    <dbReference type="OMA" id="GPMLSMI"/>
    <dbReference type="OrthoDB" id="5967390at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231:SF15">
        <property type="entry name" value="2-OXOGLUTARATE RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231">
        <property type="entry name" value="PURINOCEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="37"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017324824.1"/>
        </source>
    </evidence>
    <sequence length="317" mass="36097" checksum="22C8BFDDBF97A31A" modified="2017-12-20" version="1">MELYDNDSGVCLSVDLSIKHYYLPAMYGIIFLVGFTGNLMAIIIYVVKLRPWKTGSIIMVNLAVSDLLYALSLPFLVNFYITRNWMLGEFMCRFLRFCFHFNLYGSILFLTCLSVFRYVVVVHPLRAAQVQRACWGVSACLTIWAISLLEIGPMLSMIMTKNDGNTTDCLDFASNEPETVWWYGWLLSVLGYLLPLVVVCWCYTQIRGALSNSLSASRPSRVRAYRLTVMILAVFVMCFLPYHILRLLRVGSLRSNVTSCLQKKIINDAYTLSRPLAGLNTFFNLGLFTLAGDKFQQAFYSLIFRRKPTISKVAVIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R634</accession>
    <name>A0A2D0R634_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ankyrin repeat and SOCS box protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">asb11</name>
        <name evidence="10" type="synonym">ASB5</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ankyrin SOCS box (ASB) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017325516.1">
        <property type="nucleotide sequence ID" value="XM_017470027.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R634"/>
    <dbReference type="GeneID" id="108266553"/>
    <dbReference type="CTD" id="140456"/>
    <dbReference type="OrthoDB" id="3246549at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045732">
        <property type="term" value="P:positive regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03716">
        <property type="entry name" value="SOCS_ASB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000001">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051573">
        <property type="entry name" value="Ankyrin-SOCS_box_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24136:SF14">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24136">
        <property type="entry name" value="SOWAH (DROSOPHILA) HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="69"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="134"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="8">
        <location>
            <begin position="228"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017325516.1"/>
        </source>
    </evidence>
    <sequence length="277" mass="30009" checksum="F6447312970FA4D1" modified="2017-12-20" version="1">MAAAHAEVCVWNELWQQDFQVYGGRVCNTLMEGSWADRTPLHDSALQGRLLPIKKFLSQGMNVGVATLDGITPLHEACLGGHFACAKLLLEHGADANAVSVDGATPLFNACFSGNTALLRLLLKHSSVHHPAHLRNSPIHAAAKQGHTACVELLLSYGVDADMELDEVGTPLYCACETRSTECVQRLLILAVRVGGAKEVALLLEHGADGKCRNSEGKTPLDLAIDNSIQHLLQTAGPWSLSQLSRLCIRRSLGQKRLNSARGLQLPDILHTYILYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R638</accession>
    <name>A0A2D0R638_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Arginine and glutamate-rich protein 1-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">arglu1a</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARGLU1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017325270.1">
        <property type="nucleotide sequence ID" value="XM_017469781.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R638"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000002142"/>
    <dbReference type="GeneID" id="108266450"/>
    <dbReference type="KEGG" id="ipu:108266450"/>
    <dbReference type="CTD" id="406834"/>
    <dbReference type="OMA" id="RMEVERY"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033371">
        <property type="entry name" value="ARGLU1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31711">
        <property type="entry name" value="ARGININE AND GLUTAMATE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31711:SF1">
        <property type="entry name" value="ARGININE AND GLUTAMATE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15346">
        <property type="entry name" value="ARGLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="231"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="62"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="182"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="231"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00049652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017325270.1"/>
        </source>
    </evidence>
    <sequence length="267" mass="32299" checksum="78229900329C820D" modified="2017-12-20" version="1">MGRSRSRSSSRPKHTKSGSKHSKKRGSRSKSRSRDRERSKKRSRSREAKRNRRRESRSRSRSNRERARPTSPVDRVDMFGRTLSKRTAAEEKQRKEEEEKKAEMERQRKIRQQEIEERLIEEETARRVEELVARRVEEELEKRRDEIEREVLRRVEEAKRIMEAQLLQELERQRQAELSAQKAREEEEKSKREELEKILEENNKKIAEAQAKLAEDQLRIVEEQRKIHEERMKLEQERQRQQKEEQKIILGKGKSRPRLSFTLKATD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R9A3</accession>
    <name>A0A2D0R9A3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Hexosyltransferase</fullName>
            <ecNumber evidence="14">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">b3gnt5b</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a beta-D-Gal-(1-&gt;4)-beta-D-Glc-(1&lt;-&gt;1)-Cer(d18:1(4E)) + UDP-N-acetyl-alpha-D-glucosamine = a beta-D-GlcNAc-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Glc-(1&lt;-&gt;1)-Cer(d18:1(4E)) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13905"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17103"/>
            <dbReference type="ChEBI" id="CHEBI:17950"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="EC" id="2.4.1.206"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:13906"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a neolactoside nLc4Cer(d18:1(4E)) + UDP-N-acetyl-alpha-D-glucosamine = a neolactoside IV(3)-beta-GlcNAc-nLc4Cer(d18:1(4E)) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23004"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17006"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:142448"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:23005"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Golgi apparatus membrane</location>
            <topology evidence="1 14">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="14"/>
    <dbReference type="RefSeq" id="XP_017326656.1">
        <property type="nucleotide sequence ID" value="XM_017471167.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R9A3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000014026"/>
    <dbReference type="GeneID" id="108267178"/>
    <dbReference type="KEGG" id="ipu:108267178"/>
    <dbReference type="CTD" id="791470"/>
    <dbReference type="OMA" id="VQLFATC"/>
    <dbReference type="OrthoDB" id="115198at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047256">
        <property type="term" value="F:lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000019">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF21">
        <property type="entry name" value="LACTOSYLCERAMIDE 1,3-N-ACETYL-BETA-D-GLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 14" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017326656.1"/>
        </source>
    </evidence>
    <sequence length="378" mass="44009" checksum="6784E8AB3103A8A9" modified="2017-12-20" version="1">MFVCFRRIRKCQLRQLMMMCFVLSVIKVYWESLDNNIGGHVKSYSFRLLNSLDFISANLTISHEEAQKFSNHRYLINHEQKCVNQDVLLLLLVKSSPENFERRQAIRSTWGNEVYIEKNLGLTVRVLFALGIHPQPMDRKKLQQRLLWEDQIYQDLIQQDFLDTFHNLTVKLLLQLSWTHAYCNHARFLMSADDDIFVHMPNLIRYLQEVDQTGVKDFWIGKVHRGAPPIRKKDSKYYVSHEMYPWILYPDYTPGAGYVVSRDVASRVYQASLTLNASLYIDDVFMGICANAIGISPQEHVFFSGEGRAPNHVCIYNQMMTSHGHVKDFHELWKLATDPEVQRVSSGLFGKLYCTITKIRLLCVLHTLNTYPCKAAFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RBC1</accession>
    <name>A0A2D0RBC1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exocyst complex component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">exoc6b</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the SEC15 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017327160.1">
        <property type="nucleotide sequence ID" value="XM_017471671.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RBC1"/>
    <dbReference type="GeneID" id="108267533"/>
    <dbReference type="KEGG" id="ipu:108267533"/>
    <dbReference type="CTD" id="23233"/>
    <dbReference type="OrthoDB" id="10267033at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090522">
        <property type="term" value="P:vesicle tethering involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.30:FF:000001">
        <property type="entry name" value="Exocyst complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.670:FF:000001">
        <property type="entry name" value="Exocyst complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.670">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.30">
        <property type="entry name" value="Exocyst complex subunit Sec15 C-terminal domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007225">
        <property type="entry name" value="EXOC6/Sec15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046361">
        <property type="entry name" value="EXOC6/Sec15_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042045">
        <property type="entry name" value="EXOC6/Sec15_C_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048359">
        <property type="entry name" value="EXOC6_Sec15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042044">
        <property type="entry name" value="EXOC6PINT-1/Sec15/Tip20_C_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12702:SF3">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12702">
        <property type="entry name" value="SEC15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20651">
        <property type="entry name" value="EXOC6_Sec15_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04091">
        <property type="entry name" value="Sec15_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025007">
        <property type="entry name" value="Sec15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component EXOC6/Sec15 N-terminal" evidence="7">
        <location>
            <begin position="44"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex subunit EXOC6/Sec15 C-terminal" evidence="6">
        <location>
            <begin position="410"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="233"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="236"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR025007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017327160.1"/>
        </source>
    </evidence>
    <sequence length="810" mass="93007" checksum="5FEFE1B7B0A02034" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RBF2</accession>
    <name>A0A2D0RBF2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2-oxoglutarate and iron-dependent oxygenase JMJD4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">JmjC domain-containing protein 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Jumonji domain-containing protein 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Lysyl-hydroxylase JMJD4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">jmjd4</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-lysyl-[protein] + 2-oxoglutarate + O2 = 4-hydroxy-L-lysyl-[protein] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:57156"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:15084"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:141495"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the JMJD6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017327877.1">
        <property type="nucleotide sequence ID" value="XM_017472388.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RBF2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008978"/>
    <dbReference type="GeneID" id="108267893"/>
    <dbReference type="KEGG" id="ipu:108267893"/>
    <dbReference type="CTD" id="65094"/>
    <dbReference type="OMA" id="HPCMFSR"/>
    <dbReference type="OrthoDB" id="203487at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140096">
        <property type="term" value="F:catalytic activity, acting on a protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045905">
        <property type="term" value="P:positive regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000030">
        <property type="entry name" value="JmjC domain-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
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        <name evidence="13" type="primary">LOC108267931</name>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
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    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <tissue evidence="13">Blood</tissue>
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    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="37"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="10">
        <location>
            <begin position="159"/>
            <end position="199"/>
        </location>
    </feature>
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        <location>
            <begin position="366"/>
            <end position="561"/>
        </location>
    </feature>
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        <location>
            <begin position="197"/>
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        </location>
    </feature>
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        <location>
            <begin position="260"/>
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        </location>
    </feature>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017327994.1"/>
        </source>
    </evidence>
    <sequence length="565" mass="64787" checksum="60FA625FC5FAFCD8" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RCB5</accession>
    <name>A0A2D0RCB5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Insulin-like 5b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">insl5b</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Heterodimer of a B chain and an A chain linked by two disulfide bonds.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017328182.1">
        <property type="nucleotide sequence ID" value="XM_017472693.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A2D0RCB5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019531"/>
    <dbReference type="GeneID" id="108268041"/>
    <dbReference type="KEGG" id="ipu:108268041"/>
    <dbReference type="CTD" id="100008569"/>
    <dbReference type="OrthoDB" id="9443437at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="CDD" id="cd04365">
        <property type="entry name" value="IlGF_relaxin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.100.10">
        <property type="entry name" value="Insulin-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR016179">
        <property type="entry name" value="Insulin-like"/>
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    <dbReference type="InterPro" id="IPR051777">
        <property type="entry name" value="Insulin-like_neuro_ligands"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036438">
        <property type="entry name" value="Insulin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20968">
        <property type="entry name" value="ILGF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20968:SF2">
        <property type="entry name" value="INSULIN-LIKE PEPTIDE INSL5"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00078">
        <property type="entry name" value="IlGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56994">
        <property type="entry name" value="Insulin-like"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0372">Hormone</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Insulin-like" evidence="6">
        <location>
            <begin position="27"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017328182.1"/>
        </source>
    </evidence>
    <sequence length="93" mass="10501" checksum="6A36D57F64DD3DA8" modified="2017-12-20" version="1">MKVLLLVYVLASLLVCTALTEASQISMRLCGREFLRAVVYTCGGSRWRRSLNEIFTQDSEPEDTLRFRKSEESALLDLCCSVGCRKSDLTLMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0REZ6</accession>
    <name>A0A2D0REZ6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Macrophage mannose receptor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">mrc1a</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017328465.2">
        <property type="nucleotide sequence ID" value="XM_017472976.3"/>
    </dbReference>
    <dbReference type="GeneID" id="108268171"/>
    <dbReference type="KEGG" id="ipu:108268171"/>
    <dbReference type="CTD" id="100286774"/>
    <dbReference type="OrthoDB" id="6356110at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005537">
        <property type="term" value="F:D-mannose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23407">
        <property type="entry name" value="beta-trefoil_Ricin_MRC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="CDD" id="cd00062">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.10.10:FF:000001">
        <property type="entry name" value="Fibronectin 1a isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000032">
        <property type="entry name" value="macrophage mannose receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000014">
        <property type="entry name" value="Macrophage mannose receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000016">
        <property type="entry name" value="macrophage mannose receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000023">
        <property type="entry name" value="Macrophage mannose receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000031">
        <property type="entry name" value="macrophage mannose receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000025">
        <property type="entry name" value="Mannose receptor C-type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000030">
        <property type="entry name" value="Mannose receptor C-type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000027">
        <property type="entry name" value="Mannose receptor, C type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.10.10">
        <property type="entry name" value="Fibronectin, type II, collagen-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000562">
        <property type="entry name" value="FN_type2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036943">
        <property type="entry name" value="FN_type2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24562">
        <property type="entry name" value="CysR_MRC2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00040">
        <property type="entry name" value="fn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00013">
        <property type="entry name" value="FNTYPEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00059">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00023">
        <property type="entry name" value="FN2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51092">
        <property type="entry name" value="FN2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0430">Lectin</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14 23" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039934395" description="Macrophage mannose receptor 1" evidence="19">
        <location>
            <begin position="24"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1388"/>
            <end position="1411"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-II" evidence="21">
        <location>
            <begin position="167"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="20">
        <location>
            <begin position="226"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="20">
        <location>
            <begin position="368"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="20">
        <location>
            <begin position="510"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="20">
        <location>
            <begin position="661"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="20">
        <location>
            <begin position="800"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="20">
        <location>
            <begin position="941"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="20">
        <location>
            <begin position="1108"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="20">
        <location>
            <begin position="1248"/>
            <end position="1363"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="172"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="186"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00071860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017328465.2"/>
        </source>
    </evidence>
    <sequence length="1455" mass="164395" checksum="ED1E00799F8BF7E7" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RFW2</accession>
    <name>A0A2D0RFW2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Anoctamin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">ano10a</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the anoctamin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017329424.1">
        <property type="nucleotide sequence ID" value="XM_017473935.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053538182.1">
        <property type="nucleotide sequence ID" value="XM_053682207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RFW2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000014338"/>
    <dbReference type="GeneID" id="108268765"/>
    <dbReference type="KEGG" id="ipu:108268765"/>
    <dbReference type="CTD" id="566425"/>
    <dbReference type="OMA" id="YENHRTA"/>
    <dbReference type="OrthoDB" id="296386at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF40">
        <property type="entry name" value="ANOCTAMIN-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="502"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="591"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="7">
        <location>
            <begin position="200"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU280814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017329424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053538182.1"/>
        </source>
    </evidence>
    <sequence length="660" mass="76444" checksum="2CEF245D5AB72838" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RGK7</accession>
    <name>A0A2D0RGK7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cilia- and flagella-associated protein 263</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ccdc113</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CFAP263 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017329677.1">
        <property type="nucleotide sequence ID" value="XM_017474188.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RGK7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012573"/>
    <dbReference type="GeneID" id="108268875"/>
    <dbReference type="KEGG" id="ipu:108268875"/>
    <dbReference type="CTD" id="29070"/>
    <dbReference type="OMA" id="MFESFIC"/>
    <dbReference type="OrthoDB" id="10259713at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051885">
        <property type="entry name" value="CC_CF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025254">
        <property type="entry name" value="CCDC113/CCDC96_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15654:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15654">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 113-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13870">
        <property type="entry name" value="CCDC113_CCDC96_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCDC113/CCDC96 coiled-coil" evidence="8">
        <location>
            <begin position="163"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="344"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017329677.1"/>
        </source>
    </evidence>
    <sequence length="354" mass="41992" checksum="0D81FA68EF44416D" modified="2017-12-20" version="1">MAESKEPVTKAALKQQVEELRHSNAVLQTENEIFESFLNRLDSLSLDSQLALPQSKKGAGQRGRFERCRPSQKPPRLLNQEQKYCIAMCEMEETRKELEQYRISLARNAEYYKAAIEDSELRLAEIKRERHHFEEFVDKIRQTTWVREQRAQKVIGYIGRKIKAKETLTERMHRRNKFLQMQNLKQQRMVHQKEQMQETPSWVDLEHLKQMNTQCQKRLQKFQAQLLNQRMLVRKGRQSLNVYKERLKKEMQESESLSRKIASHEERLSKIREKQQKVEKERVQAEALNKKLKAQLANFRVPDVMKYVEAKATNDRLRQTVKSLERKARVAEMALKTKKKSPEESSGCSRSSDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RJB6</accession>
    <name>A0A2D0RJB6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Olfactomedin-like protein 3A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">olfml1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017330617.1">
        <property type="nucleotide sequence ID" value="XM_017475128.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RJB6"/>
    <dbReference type="GeneID" id="108269321"/>
    <dbReference type="KEGG" id="ipu:108269321"/>
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    <dbReference type="OrthoDB" id="8626508at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003112">
        <property type="entry name" value="Olfac-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050605">
        <property type="entry name" value="Olfactomedin-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192:SF13">
        <property type="entry name" value="OLFACTOMEDIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192">
        <property type="entry name" value="OLFACTOMEDIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02191">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00284">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51132">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012338770" evidence="4">
        <location>
            <begin position="19"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Olfactomedin-like" evidence="5">
        <location>
            <begin position="135"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="136"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017330617.1"/>
        </source>
    </evidence>
    <sequence length="396" mass="45247" checksum="D1DED1670433F8D0" modified="2017-12-20" version="1">MFLKLLVMLFMSLCAIKAQRSTQDVFMMEYFQRRILEMEERLISCNQDLQSMNQRMYDMSAEMRGQVRSVNVLKSEIEGYVDSLATRVDRVERDVEYLHTKLPDTSSVEISDSLLDQQVKEAQSKRKAVIIQGKECSTRFSGIKSQKIVKKAGDVVGSWLKDPTVDSSKIYFFSGSRNNTLLQFGSLKSFTDSNSTTKHKVIQLPRPWQGTGHAVYDGFVYYHNADTRNEVLKVSLVNRTVADRMLLPAAGRIPTYGLAPHTFLDFAVDELGLWVIHADPDFGGNLVITKLDSRSLSVEHTWDTTCKSHNAEGAFIVCGTMYVVYNSHSGGRSSIQCLFDIHDSVWTEELPVMYFPKRYSSHSSMHYHPQDKQIYAWDDGYQTMYQLDVKKKLQVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RM46</accession>
    <name>A0A2D0RM46_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein TALPID3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">kiaa0586</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017331216.1">
        <property type="nucleotide sequence ID" value="XM_017475727.3"/>
    </dbReference>
    <dbReference type="GeneID" id="108269750"/>
    <dbReference type="KEGG" id="ipu:108269750"/>
    <dbReference type="CTD" id="9786"/>
    <dbReference type="OrthoDB" id="10057439at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007224">
        <property type="term" value="P:smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029246">
        <property type="entry name" value="TALPID3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15721">
        <property type="entry name" value="KIAA0586 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15721:SF2">
        <property type="entry name" value="PROTEIN TALPID3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15324">
        <property type="entry name" value="TALPID3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="345"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="535"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="676"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="939"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1148"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1448"/>
            <end position="1472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1538"/>
            <end position="1589"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="590"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="402"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="413"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="538"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="548"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="678"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="699"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="725"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="978"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1013"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1171"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1276"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1570"/>
            <end position="1579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1580"/>
            <end position="1589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017331216.1"/>
        </source>
    </evidence>
    <sequence length="1589" mass="172455" checksum="AD71C0ADF4BF7369" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S107</accession>
    <name>A0A2D0S107_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PRKCA-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Protein interacting with C kinase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Protein kinase C-alpha-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28 29" type="primary">pick1</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">SDA103</strain>
        </source>
    </reference>
    <reference evidence="28 29" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="28 29">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate ASIC1/ASIC3 channel. Regulates actin polymerization by inhibiting the actin-nucleating activity of the Arp2/3 complex; the function is competitive with nucleation promoting factors and is linked to neuronal morphology regulation and AMPA receptor (AMPAR) endocytosis. Via interaction with the Arp2/3 complex involved in regulation of synaptic plasicity of excitatory synapses and required for spine shrinkage during long-term depression (LTD). Involved in regulation of astrocyte morphology, antagonistic to Arp2/3 complex activator WASL/N-WASP function.</text>
    </comment>
    <comment type="subunit">
        <text evidence="23">Monomer and homodimer. Interacts with CXADR. Interacts presynaptically with the glutamate receptors GRIA2, GRIA3, GRIK3, isoform 3 of GRIA4, isoform A of GRM4, GRM7 and GRM8; with NAPA and NAPB; and with BTG2. The interaction with NAPA and NAPB disrupts the interaction with GRIA2, conducting to the internalization of GRIA2. Interacts with PRKCA; with the amine transporters SLC6A2 and SLC6A3; with the channels ASIC1 and ASIC2; with the GTP-binding proteins ARF1 and ARF3; with the ephrin receptor tyrosine kinases EPHA7, EPHB1 and EPHB2; with ERBB2 and through its PDZ domain with the C-terminal tail of PRLHR. Interacts with UNC5A. Interacts (via AH domain) with NCS1/FREQ; in a calcium-dependent manner. Interacts with F-actin and associates with the ARP2/3 complex. Interacts (via PDZ domain) with ARF1 (activated); the interaction blocks Arp2/3 complex inhibition. Interacts with SORCS3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Postsynaptic density</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Synapse</location>
            <location evidence="21">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017336429.1">
        <property type="nucleotide sequence ID" value="XM_017480940.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017336430.1">
        <property type="nucleotide sequence ID" value="XM_017480941.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S107"/>
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        <property type="term" value="C:perinuclear region of cytoplasm"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="GOC"/>
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        <property type="term" value="C:postsynaptic density"/>
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        <property type="term" value="C:postsynaptic early endosome"/>
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        <property type="term" value="C:synaptic vesicle"/>
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        <property type="term" value="F:actin binding"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="term" value="F:protein domain specific binding"/>
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        <property type="term" value="F:protein kinase C binding"/>
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        <property type="term" value="P:dendritic spine maintenance"/>
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        <property type="term" value="P:intracellular protein transport"/>
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        <property type="term" value="P:positive regulation of receptor internalization"/>
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        <property type="term" value="P:receptor clustering"/>
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    <dbReference type="GO" id="GO:0034315">
        <property type="term" value="P:regulation of Arp2/3 complex-mediated actin nucleation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07659">
        <property type="entry name" value="BAR_PICK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06722">
        <property type="entry name" value="PDZ_PICK1-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Interacting with PRKCA"/>
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        <property type="entry name" value="Interacting with PRKCA"/>
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        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
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        <property type="entry name" value="AH/BAR_dom_sf"/>
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        <property type="entry name" value="Arfaptin_fam"/>
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        <property type="entry name" value="PDZ"/>
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        <property type="entry name" value="PDZ_sf"/>
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        <property type="entry name" value="PICK1_BAR"/>
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        <property type="entry name" value="ARFAPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12141:SF1">
        <property type="entry name" value="PRKCA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06456">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01015">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="17" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0564">Palmitate</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0770">Synapse</keyword>
    <keyword evidence="8" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDZ" evidence="25">
        <location>
            <begin position="23"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="AH" evidence="26">
        <location>
            <begin position="145"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="370"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="24">
        <location>
            <begin position="377"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="390"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
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            <dbReference type="ARBA" id="ARBA00004635"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00017975"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
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    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023288"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00032804"/>
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            <dbReference type="ARBA" id="ARBA00034105"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00093501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_017336429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_017336430.1"/>
        </source>
    </evidence>
    <sequence length="408" mass="46179" checksum="B94B8AD26AD2B63E" modified="2017-12-20" version="1">MFSDTMDYELEEDKLGIPTVPGTVTLKKDAQNLIGISIGGGAQYCPCLYIVQVFDNTPAALDGTLAAGDEITGVNGKPVKGKTKVEVAKMIQAVQGEAVIHYNKLQADPKQGKSLDIVLKKVKHRLVENMSSGTADALGLSRAILCNDGLVKKLDELEKTAELYKGLMEHSKQLLRAFFELSQTHRAFGDVFSVIGVREPQAAASEAFVKFAEAHRNIEKYGIQLLKTIKPMLHDLNTYLHKAIPDTKLTIRKYLDVKFEYLSYCLKVKEMDDEEYSCIALGEPLYRVSTGNYEYRLILRCRQEARSRFAKMRKDVLEKIELLDQKHVQDIVFQLQRFVSGMSRYYDECYAVLKEADVFPIEVDLSRTTLNYTGPNYEEEDEEGEEERGEEEKNEQTENGAEKLIDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S4A7</accession>
    <name>A0A2D0S4A7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Arf-GAP with dual PH domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">zgc:92360</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017337271.1">
        <property type="nucleotide sequence ID" value="XM_017481782.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S4A7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000025773"/>
    <dbReference type="GeneID" id="108272920"/>
    <dbReference type="KEGG" id="ipu:108272920"/>
    <dbReference type="OMA" id="KNCWEES"/>
    <dbReference type="OrthoDB" id="73919at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902936">
        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005547">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08832">
        <property type="entry name" value="ArfGap_ADAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13252">
        <property type="entry name" value="PH1_ADAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01251">
        <property type="entry name" value="PH2_ADAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000011">
        <property type="entry name" value="Arf-GAP with dual PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000080">
        <property type="entry name" value="Arf-GAP with dual PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000099">
        <property type="entry name" value="Arf-GAP with dual PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052589">
        <property type="entry name" value="Arf-GAP_dual-PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037849">
        <property type="entry name" value="PH1_ADAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037851">
        <property type="entry name" value="PH2_ADAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46021:SF3">
        <property type="entry name" value="ARF-GAP WITH DUAL PH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46021">
        <property type="entry name" value="ARF-GAP WITH DUAL PH DOMAIN-CONTAINING PROTEIN 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="10">
        <location>
            <begin position="5"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="9">
        <location>
            <begin position="131"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="9">
        <location>
            <begin position="257"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017337271.1"/>
        </source>
    </evidence>
    <sequence length="380" mass="43867" checksum="CF6D0C0B9EA36F78" modified="2017-12-20" version="1">MAVNERAARALRDIMKRQGNGTCADCGAPDPEWGSCSLGVFICLTCSGIHRNIPNIGKVKSVLLSHWEDSEVQFMAENGNEVSKSKFEAYVPAYYYKPTHKDCQVLKEQWIRAKYERQEFIQDSKPLVYEEGTRDGVLMKRGRDNGQFLSRRFVLSEREGTLKYYTKFDAKEVNKAKAVIKVDTINASFQPEKIGNPNGLQITYLKDYNTRNIFLYHDNSKEIVDWYNSIRAVQLHYLKVAFPGASELELKPKLTRNFLKEGYMEKTGPRQTEGFKKRWFTLDHRRLMYFKDPLDAFAKGEVFLGHRDLGYSASPGLPASTQSNGTWQHGITIVTPERSFLFTCETEAEQLDWLKHFNHVISLQMSPQEYSMEALFKHKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S6I7</accession>
    <name>A0A2D0S6I7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Monocarboxylate transporter 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">slc16a8</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017338337.1">
        <property type="nucleotide sequence ID" value="XM_017482848.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S6I7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000001930"/>
    <dbReference type="GeneID" id="108273568"/>
    <dbReference type="KEGG" id="ipu:108273568"/>
    <dbReference type="CTD" id="23539"/>
    <dbReference type="OrthoDB" id="6499973at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008028">
        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17430">
        <property type="entry name" value="MFS_MCT3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000077">
        <property type="entry name" value="Proton-coupled monocarboxylate transporter 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004743">
        <property type="entry name" value="MCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00892">
        <property type="entry name" value="2A0113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF26">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="252"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="379"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="410"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="19"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017338337.1"/>
        </source>
    </evidence>
    <sequence length="470" mass="50837" checksum="1358F9F64DA634E7" modified="2017-12-20" version="1">MGSSVVERNRVDPPDGGWGWAVLFGGFIITGFSYAFPKAISVYFKELMRDFGCGYSDTAWISSIMLAMLYGSGPLSSIMVNRFGCRPVMLIGGLFASAGMIGASFTTNIIQLYLTAGVITGLGLALNFQPSLIMLGCYFDKRRPLANGLAAAGSPVFLSALSPLGQVLMDRFGWRGGFLIMGGLLLNCCICGAVMRPLGYKVRKSTTRDESNAHELKEMLPSKEENNLEKAQKESPKKKKLLDFSVLRDKGLIIYIIAKFVVVLGLFVPTILLVNYAKDQGVPDQEAAFLLSIIGFIDIFARPTCGLMAGVKCIRAKMPYFFSLALILNGLTDVCSARSTDYMGLVIFCVFFGLSYGMVGALQFEVLMGIVGTNSFSSALGLVLLIEAAAVLIGPPSAGRLVDTYKNYELIFYMAGGELMAAGIFLAIASYCCIKLQKNKDKSVIQEADIEGNSNHINHESNHAMNNSSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S6Q0</accession>
    <name>A0A2D0S6Q0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cytohesin-interacting protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">si:dkey-276j7.2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017337895.2">
        <property type="nucleotide sequence ID" value="XM_017482406.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S6Q0"/>
    <dbReference type="GeneID" id="108273252"/>
    <dbReference type="KEGG" id="ipu:108273252"/>
    <dbReference type="OrthoDB" id="10041077at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052122">
        <property type="entry name" value="Intracell_Traff_Signaling_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15963:SF1">
        <property type="entry name" value="CYTOHESIN-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15963">
        <property type="entry name" value="GENERAL RECEPTOR FOR PHOSPHOINOSITIDES 1-ASSOCIATED SCAFFOLD PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="47"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="137"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017337895.2"/>
        </source>
    </evidence>
    <sequence length="197" mass="22246" checksum="A4AC380376647BF2" modified="2023-06-28" version="2">MTMSSVMLIKKLLRKSSRAGCVAREGQRSTAMMTQIKHKTHYSERQNVSISKKDNEAFGFNIRTYEKNTTDAELFTCVCSVKENSPAENAGLRTGDIIISVNSICVEGLQHEQIVDLVQKGSCLLKMEVVRGTSVKQKELQMKLEHLQRHLREKRAELQMLITQEERLRGGELRCTQPRSCLASENSTLSSCLVLQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SD14</accession>
    <name>A0A2D0SD14_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Cyclin-dependent kinase inhibitor 1C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">cdkn1cb</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017340614.1">
        <property type="nucleotide sequence ID" value="XM_017485125.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017340615.1">
        <property type="nucleotide sequence ID" value="XM_017485126.3"/>
    </dbReference>
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    <dbReference type="STRING" id="7998.ENSIPUP00000022125"/>
    <dbReference type="GeneID" id="108274779"/>
    <dbReference type="KEGG" id="ipu:108274779"/>
    <dbReference type="CTD" id="100004175"/>
    <dbReference type="OMA" id="NFNFGAG"/>
    <dbReference type="OrthoDB" id="6373236at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004861">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045930">
        <property type="term" value="P:negative regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.365.10">
        <property type="entry name" value="p27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003175">
        <property type="entry name" value="CDI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044898">
        <property type="entry name" value="CDI_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10265">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10265:SF44">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02234">
        <property type="entry name" value="CDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 9 10" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-dependent kinase inhibitor" evidence="7">
        <location>
            <begin position="29"/>
            <end position="75"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="104"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="125"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="171"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017340614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017340615.1"/>
        </source>
    </evidence>
    <sequence length="191" mass="21238" checksum="7C8B6C79BF8B9574" modified="2017-12-20" version="1">MSSVQFSVSGSGAARESFPQPARRSVCRSLFGPVDHDELNRDIEAKLREISDRDQKRWNFNFVSGTPLLGDYEWEGAAVEATPAFYQESVQVGKNRAVVLNVKPRTDAPNRENSAQDAAAGLTVSPESASARKPSSARRKTSTRLTTAAPHRITDFYAKRMKQGDLKRSESAPQNPLSFRSSEQTPRKRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SE30</accession>
    <name>A0A2D0SE30_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Golgi apparatus protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">glg1a</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Golgi outpost</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017340983.1">
        <property type="nucleotide sequence ID" value="XM_017485494.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SE30"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000024983"/>
    <dbReference type="GeneID" id="108274991"/>
    <dbReference type="KEGG" id="ipu:108274991"/>
    <dbReference type="CTD" id="792865"/>
    <dbReference type="OrthoDB" id="2015434at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017134">
        <property type="term" value="F:fibroblast growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001893">
        <property type="entry name" value="Cys-rich_GLG1_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017873">
        <property type="entry name" value="Cys-rich_GLG1_repeat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039728">
        <property type="entry name" value="GLG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11884:SF1">
        <property type="entry name" value="GOLGI APPARATUS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11884">
        <property type="entry name" value="SELECTIN LIGAND RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00839">
        <property type="entry name" value="Cys_rich_FGFR"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51289">
        <property type="entry name" value="GLG1_C_RICH"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0730">Sialic acid</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012677626" description="Golgi apparatus protein 1" evidence="14">
        <location>
            <begin position="26"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1115"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="repeat" description="Cys-rich GLG1" evidence="11">
        <location>
            <begin position="245"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="repeat" description="Cys-rich GLG1" evidence="11">
        <location>
            <begin position="374"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="repeat" description="Cys-rich GLG1" evidence="11">
        <location>
            <begin position="496"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="repeat" description="Cys-rich GLG1" evidence="11">
        <location>
            <begin position="578"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="repeat" description="Cys-rich GLG1" evidence="11">
        <location>
            <begin position="698"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="repeat" description="Cys-rich GLG1" evidence="11">
        <location>
            <begin position="765"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="repeat" description="Cys-rich GLG1" evidence="11">
        <location>
            <begin position="881"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="repeat" description="Cys-rich GLG1" evidence="11">
        <location>
            <begin position="1010"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017340983.1"/>
        </source>
    </evidence>
    <sequence length="1171" mass="134408" checksum="6FF704E8D369A6C7" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SG62</accession>
    <name>A0A2D0SG62_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ETS homologous factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">ETS domain-containing transcription factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ehf</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017341699.1">
        <property type="nucleotide sequence ID" value="XM_017486210.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SG62"/>
    <dbReference type="GeneID" id="108275415"/>
    <dbReference type="CTD" id="26298"/>
    <dbReference type="OrthoDB" id="5961210at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000136">
        <property type="entry name" value="ETS homologous factor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003118">
        <property type="entry name" value="Pointed_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF171">
        <property type="entry name" value="ETS HOMOLOGOUS FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02198">
        <property type="entry name" value="SAM_PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00251">
        <property type="entry name" value="SAM_PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51433">
        <property type="entry name" value="PNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PNT" evidence="12">
        <location>
            <begin position="35"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="ETS" evidence="11">
        <location>
            <begin position="195"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="167"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017341699.1"/>
        </source>
    </evidence>
    <sequence length="288" mass="33542" checksum="C1A7C7B0AB337A95" modified="2017-12-20" version="1">MVLHSGCSMSIPASMSVESQLPTSWSPHYNSPDVSMVSPGYASRLWSQDLHPQYWSKYQVWEWLQQTLDMHQIDATSIPFSNFDLDGRQLSSMSYHDFTHLAGSVGPVLYQSLTDLKWSSQYSSSADMFTPGDIKTELDVLPYKEEPSFYATSEHLDIKTQFQPTVMTSPASYSPDPKRPHSRSHQVKKHNPRGTHLWEFIRDILLNPERNPGLIKWENRSEGVFRFLKSEAVAQLWGKKKNNSSMTYEKLSRAMRYYYKREILERVDGRRLVYKFGRNARGWRETEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SJR7</accession>
    <name>A0A2D0SJR7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Keratin, type II cytoskeletal 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">krt5</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017342932.2">
        <property type="nucleotide sequence ID" value="XM_017487443.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SJR7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009874"/>
    <dbReference type="GeneID" id="108276093"/>
    <dbReference type="KEGG" id="ipu:108276093"/>
    <dbReference type="CTD" id="3852"/>
    <dbReference type="OrthoDB" id="2441647at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030280">
        <property type="term" value="F:structural constituent of skin epidermis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031424">
        <property type="term" value="P:keratinization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000001">
        <property type="entry name" value="Keratin type II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000004">
        <property type="entry name" value="Keratin, type II cytoskeletal 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032444">
        <property type="entry name" value="Keratin_2_head"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003054">
        <property type="entry name" value="Keratin_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616">
        <property type="entry name" value="GATA-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616:SF21">
        <property type="entry name" value="KERATIN, TYPE II CYTOSKELETAL 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16208">
        <property type="entry name" value="Keratin_2_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01276">
        <property type="entry name" value="TYPE2KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
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    </dbReference>
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        <location>
            <begin position="179"/>
            <end position="490"/>
        </location>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="183"/>
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        </location>
    </feature>
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        <location>
            <begin position="388"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017342932.2"/>
        </source>
    </evidence>
    <sequence length="599" mass="61570" checksum="09E2101158F62365" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SK41</accession>
    <name>A0A2D0SK41_ICTPU</name>
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        <submittedName>
            <fullName evidence="18 19">Toll-like receptor 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18 19" type="primary">LOC108275971</name>
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    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18 19" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18 19">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Toll-like receptor family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017487181.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017342677.1">
        <property type="nucleotide sequence ID" value="XM_017487188.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SK41"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000016467"/>
    <dbReference type="GeneID" id="108275971"/>
    <dbReference type="KEGG" id="ipu:108275971"/>
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    <dbReference type="OrthoDB" id="6160824at2759"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002224">
        <property type="term" value="P:toll-like receptor signaling pathway"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="GD11101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000001">
        <property type="entry name" value="Toll-like receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Toll-like receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Leu-rich_rpt"/>
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        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017241">
        <property type="entry name" value="Toll-like_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365">
        <property type="entry name" value="TOLL-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365:SF525">
        <property type="entry name" value="TOLL-LIKE RECEPTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037595">
        <property type="entry name" value="Toll-like_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="13" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013510556" evidence="15">
        <location>
            <begin position="22"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="656"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="16">
        <location>
            <begin position="707"/>
            <end position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017342670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017342677.1"/>
        </source>
    </evidence>
    <sequence length="880" mass="100447" checksum="DAE13E52530F6F9E" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SKR1</accession>
    <name>A0A2D0SKR1_ICTPU</name>
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        <recommendedName>
            <fullName evidence="5">phosphatidylinositol-4,5-bisphosphate 3-kinase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">pik3cd</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1-octadecanoyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phospho-1D-myo-inositol 4,5-bisphosphate + ATP = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phospho-(1D-myo-inositol 3,4,5-triphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43396"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:77137"/>
            <dbReference type="ChEBI" id="CHEBI:83243"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:43397"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.153"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:21293"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.153" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017343076.2">
        <property type="nucleotide sequence ID" value="XM_017487587.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SKR1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005720"/>
    <dbReference type="GeneID" id="108276156"/>
    <dbReference type="KEGG" id="ipu:108276156"/>
    <dbReference type="CTD" id="5293"/>
    <dbReference type="OrthoDB" id="67688at2759"/>
    <dbReference type="UniPathway" id="UPA00220"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005942">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016303">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046934">
        <property type="term" value="F:1-phosphatidylinositol-4,5-bisphosphate 3-kinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035005">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043491">
        <property type="term" value="P:phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08693">
        <property type="entry name" value="C2_PI3K_class_I_beta_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00872">
        <property type="entry name" value="PI3Ka_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000001">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000046">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.770:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000005">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002420">
        <property type="entry name" value="PI3K-type_C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003113">
        <property type="entry name" value="PI3K_ABD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000341">
        <property type="entry name" value="PI3K_Ras-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF35">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE 3-KINASE CATALYTIC SUBUNIT DELTA ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00792">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02192">
        <property type="entry name" value="PI3K_p85B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00794">
        <property type="entry name" value="PI3K_rbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00142">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00143">
        <property type="entry name" value="PI3K_p85B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00144">
        <property type="entry name" value="PI3K_rbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51547">
        <property type="entry name" value="C2_PI3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51544">
        <property type="entry name" value="PI3K_ABD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51546">
        <property type="entry name" value="PI3K_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K-ABD" evidence="17">
        <location>
            <begin position="17"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K-RBD" evidence="19">
        <location>
            <begin position="188"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="C2 PI3K-type" evidence="20">
        <location>
            <begin position="322"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="PIK helical" evidence="18">
        <location>
            <begin position="498"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="16">
        <location>
            <begin position="747"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017343076.2"/>
        </source>
    </evidence>
    <sequence length="1044" mass="120278" checksum="34711EF3FB891DB3" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SM30</accession>
    <name>A0A2D0SM30_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="primary">LOC108276527</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="29">SDA103</strain>
        </source>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="30">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017343762.1">
        <property type="nucleotide sequence ID" value="XM_017488273.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SM30"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000037200"/>
    <dbReference type="GeneID" id="108276527"/>
    <dbReference type="KEGG" id="ipu:108276527"/>
    <dbReference type="OMA" id="VAWTWTW"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005005">
        <property type="term" value="F:transmembrane-ephrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="TYRKINASE"/>
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            <name>ATP</name>
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        <ligand>
            <name>ATP</name>
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    <evidence type="ECO:0000313" key="30">
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    <sequence length="987" mass="110602" checksum="E11AD82E2D5FA161" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SMD5</accession>
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        <submittedName>
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        <name evidence="10" type="primary">LOC108276592</name>
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        <name type="scientific">Ictalurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
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    </organism>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9">SDA103</strain>
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    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="nucleotide sequence ID" value="XM_017488379.3"/>
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            <end position="2261"/>
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</entry>
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        <name type="scientific">Ictalurus punctatus</name>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <strain evidence="5">SDA103</strain>
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        <citation type="submission" date="2025-04" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017344864.2"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_047017053.2"/>
        </source>
    </evidence>
    <sequence length="1941" mass="214039" checksum="2077DDF4777FF50C" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SQR9</accession>
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        <recommendedName>
            <fullName evidence="2">Very low-density lipoprotein receptor</fullName>
        </recommendedName>
    </protein>
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        <name evidence="29" type="primary">vldlr</name>
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            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
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            <taxon>Siluriformes</taxon>
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        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">SDA103</strain>
        </source>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="29">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="21">Homooligomer. Binds to the extracellular matrix protein Reelin/RELN. Interacts with LRP8. Interacts with LDLRAP1. Interacts with SNX17. Interacts with DAB1. Interacts with PCSK9. Interacts with PAFAH1B3 and PAFAH1B2, the catalytic complex of (PAF-AH (I)) heterotetrameric enzyme; these interactions may modulate the Reelin pathway. Interacts with STX5; this interaction mediates VLDLR translocation from the endoplasmic reticulum to the plasma membrane. Interacts with CLU.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <location evidence="1">Clathrin-coated pit</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="22">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017345048.1">
        <property type="nucleotide sequence ID" value="XM_017489559.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SQR9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000037055"/>
    <dbReference type="GeneID" id="108277138"/>
    <dbReference type="KEGG" id="ipu:108277138"/>
    <dbReference type="CTD" id="7436"/>
    <dbReference type="OrthoDB" id="5958943at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034361">
        <property type="term" value="C:very-low-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000009">
        <property type="entry name" value="Low-density lipoprotein receptor isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000052">
        <property type="entry name" value="low-density lipoprotein receptor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000002">
        <property type="entry name" value="low-density lipoprotein receptor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000025">
        <property type="entry name" value="Very low density lipoprotein receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000038">
        <property type="entry name" value="Very low density lipoprotein receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000043">
        <property type="entry name" value="Very low density lipoprotein receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000046">
        <property type="entry name" value="Very low density lipoprotein receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000049">
        <property type="entry name" value="Very low density lipoprotein receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000053">
        <property type="entry name" value="Very low density lipoprotein receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000057">
        <property type="entry name" value="Very low density lipoprotein receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
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        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051221">
        <property type="entry name" value="LDLR-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722:SF15">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="17" id="KW-0168">Coated pit</keyword>
    <keyword evidence="14 22" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 22" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="29" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="16 29" id="KW-0675">Receptor</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 26" id="KW-0732">Signal</keyword>
    <keyword evidence="19" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="15" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="7 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 25" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="20" id="KW-0850">VLDL</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013356685" description="Very low-density lipoprotein receptor" evidence="26">
        <location>
            <begin position="24"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="800"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="27">
        <location>
            <begin position="391"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="24">
        <location>
            <begin position="521"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="24">
        <location>
            <begin position="564"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="24">
        <location>
            <begin position="608"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="47"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="67"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="129"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="149"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="168"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="188"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="234"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="253"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="273"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="292"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="321"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="395"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00064645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_017345048.1"/>
        </source>
    </evidence>
    <sequence length="876" mass="97281" checksum="20B05FBBE6B2AFC4" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SRE8</accession>
    <name>A0A2D0SRE8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">MAM domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mamdc2b</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
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                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="5">Blood</tissue>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
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    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:actin filament organization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070830">
        <property type="term" value="P:bicellular tight junction assembly"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="POF1B_HlH"/>
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    </feature>
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        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF24617"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017348189.1"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T2D4</accession>
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            <fullName evidence="10">Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase</fullName>
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    </protein>
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        <name evidence="14" type="primary">ftsj1</name>
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    <organism evidence="13 14">
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs.</text>
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    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>cytidine(32)/guanosine(34) in tRNA + 2 S-adenosyl-L-methionine = 2'-O-methylcytidine(32)/2'-O-methylguanosine(34) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42396"/>
            <dbReference type="Rhea" id="RHEA-COMP:10246"/>
            <dbReference type="Rhea" id="RHEA-COMP:10247"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74445"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.205"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with WDR6; the interaction is direct, and required for 2'-O-methylation of position 34 in substrate tRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. TRM7 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.205" evidence="10"/>
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        <property type="nucleotide sequence ID" value="XM_017493482.3"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106340">
        <property type="term" value="F:tRNA (guanosine(34)-2'-O)-methyltransferase activity"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:cytoplasmic translation"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
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        <property type="entry name" value="Putative ribosomal RNA methyltransferase 1"/>
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        <property type="entry name" value="Vaccinia Virus protein VP39"/>
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        <property type="entry name" value="RNA_methyltr_E_TRM7"/>
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    </dbReference>
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        <property type="entry name" value="RNA_methyltr_E_TRM7"/>
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        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
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        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
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        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
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        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF12">
        <property type="entry name" value="TRNA (CYTIDINE(32)_GUANOSINE(34)-2'-O)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 10" id="KW-0819">tRNA processing</keyword>
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        <location>
            <begin position="21"/>
            <end position="209"/>
        </location>
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        <location>
            <begin position="299"/>
            <end position="326"/>
        </location>
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        <location>
            <begin position="311"/>
            <end position="326"/>
        </location>
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    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="55"/>
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        <ligand>
            <name>S-adenosyl-L-methionine</name>
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        </ligand>
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        <location>
            <position position="85"/>
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        <ligand>
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        </ligand>
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        <location>
            <position position="101"/>
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        <ligand>
            <name>S-adenosyl-L-methionine</name>
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        </ligand>
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        <ligand>
            <name>S-adenosyl-L-methionine</name>
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        <source>
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        <source>
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        <source>
            <dbReference type="RefSeq" id="XP_017348971.1"/>
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    <sequence length="326" mass="36284" checksum="3772F1115F3E2AB5" modified="2017-12-20" version="1">MGRSSKDKRDIYYRLAKEEGWRARSAFKLLQLDEEFKLFKGVRRAVDLCAAPGSWSQVLSRKLRPFVRCCRVKESSEEVKIVAVDLQAMAPLPGVTQIQGDITKISTAQEIIRHFEGQAADLVVCDGAPDVTGLHDVDEYIQAQLLLAALNITSHVLKPGGNFVAKIFRGKDVTLLYSQLKIFFSTVTCAKPRSSRNSSIEAFVVCQNYSPPEGYVPNMSNPLLDHSYDVDFNQLEGPNRVIVPFLACGDLSAFDSDRTYPLQLDSEKEYEYLPPTQPPIRPPYQQACHLRKNNLLAKEDSPSAVLDGALSTMNLGSEQETDPSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T612</accession>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein C12orf56 homolog</fullName>
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        <name evidence="3" type="primary">c19h12orf56</name>
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        <name type="scientific">Ictalurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">SDA103</strain>
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="3">Blood</tissue>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017350333.1"/>
        </source>
    </evidence>
    <sequence length="543" mass="61965" checksum="00D0466FD154EA98" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T727</accession>
    <name>A0A2D0T727_ICTPU</name>
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        <recommendedName>
            <fullName evidence="6">Protein phosphatase 1 regulatory subunit 7</fullName>
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        <name type="scientific">Ictalurus punctatus</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SDS22 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017350445.1">
        <property type="nucleotide sequence ID" value="XM_017494956.3"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="7451790at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Protein phosphatase 1 regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000127">
        <property type="entry name" value="protein phosphatase 1 regulatory subunit 7 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003603">
        <property type="entry name" value="U2A'_phosphoprotein32A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF23">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00446">
        <property type="entry name" value="LRRcap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="U2A'/phosphoprotein 32 family A C-terminal" evidence="8">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00446"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017350445.1"/>
        </source>
    </evidence>
    <sequence length="292" mass="33706" checksum="B0B35F452FB7EFF9" modified="2017-12-20" version="1">MDTITLDPNEEDVDLVHCRIGKIEGLEVLQKAKTLSLRQNLIKNIENLESLVSLRELDLYDNQIRKLENLQALTELEQLDVSFNLLRKIEGLERLTKLKKLFLLHNKITNISNLEHLTGLQMLELGSNRIRIIENLDTLTSLDSLFLGTNKIAQLQNLDGLHNLTVLSIQSNRITKLEGLQNLGNLKELYLSHNGIEVIEGLENNKKLTTLDIAANRIKKIENISHLTDLQEFWMNDNQIENWSDLDELKNAKGLETVYLERNPLQKDPQYRRKIMLALPSVRQIDATFIRF</sequence>
</entry>
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    <accession>A0A979F8L3</accession>
    <name>A0A979F8L3_ICTPU</name>
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        <submittedName>
            <fullName evidence="9">Protein SOGA3</fullName>
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    </protein>
    <gene>
        <name evidence="9" type="primary">LOC108275603</name>
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    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_047016403.2">
        <property type="nucleotide sequence ID" value="XM_047160447.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979F8L3"/>
    <dbReference type="GeneID" id="108275603"/>
    <dbReference type="KEGG" id="ipu:108275603"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="MTCL1-3"/>
    </dbReference>
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        <property type="entry name" value="SOGA_CC"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN SOGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11365">
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        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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            <begin position="184"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="SOGA coiled-coil" evidence="7">
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            <begin position="308"/>
            <end position="400"/>
        </location>
    </feature>
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            <end position="30"/>
        </location>
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            <end position="561"/>
        </location>
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            <end position="681"/>
        </location>
    </feature>
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            <end position="226"/>
        </location>
    </feature>
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        <location>
            <begin position="254"/>
            <end position="396"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <end position="176"/>
        </location>
    </feature>
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            <end position="426"/>
        </location>
    </feature>
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            <end position="456"/>
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    </feature>
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        </location>
    </feature>
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            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="639"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_047016403.2"/>
        </source>
    </evidence>
    <sequence length="681" mass="77946" checksum="FB61881D1FEBFE1A" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F9W1</accession>
    <name>A0A979F9W1_ICTPU</name>
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        <submittedName>
            <fullName evidence="17">Protocadherin beta-16 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC108276785</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_047016895.1">
        <property type="nucleotide sequence ID" value="XM_047160939.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A979F9W1"/>
    <dbReference type="GeneID" id="108276785"/>
    <dbReference type="OrthoDB" id="6252479at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031904">
        <property type="entry name" value="Cadherin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF114">
        <property type="entry name" value="PCDH2G3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15974">
        <property type="entry name" value="Cadherin_tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="728"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="106"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="175"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="284"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="397"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="494"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="618"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="842"/>
            <end position="868"/>
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            <begin position="991"/>
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</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
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    <name>A0A9F7QQU3_ICTPU</name>
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            <fullName evidence="2 11">Cleavage and polyadenylation specificity factor subunit 4</fullName>
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        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cleavage and polyadenylation specificity factor 30 kDa subunit</fullName>
        </alternativeName>
    </protein>
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        <name evidence="16" type="primary">cpsf4</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. CPSF4 binds RNA polymers with a preference for poly(U).</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the cleavage and polyadenylation specificity factor (CPSF) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the CPSF4/YTH1 family.</text>
    </comment>
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    </dbReference>
    <dbReference type="GO" id="GO:0005847">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 11" id="KW-0507">mRNA processing</keyword>
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    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
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        </location>
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    </feature>
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            <begin position="203"/>
            <end position="218"/>
        </location>
    </feature>
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            <begin position="18"/>
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    </feature>
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            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
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            <begin position="74"/>
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        </location>
    </feature>
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            <end position="125"/>
        </location>
    </feature>
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            <begin position="129"/>
            <end position="151"/>
        </location>
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            <begin position="170"/>
            <end position="196"/>
        </location>
    </feature>
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        <location>
            <begin position="139"/>
            <end position="151"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00016264"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_053531020.1"/>
        </source>
    </evidence>
    <sequence length="227" mass="25804" checksum="77F886420CBACABD" modified="2023-06-28" version="1">MRSACLKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFYSKFGECSNKECPFLHIDPESKIKDCPWYDRGFCKHGPDCRHRHTRRVICVNYLVGFCPEGKSCKFMHPRFELPMGTNEQPPLPQQAQTQQKQNAQNLNRSSLSLIQLTSNNSIANINHQKPPHILGASQPQNNTGGSRGPRPLDQVTCYKCGEKGHYANKCTKGHLAFLSGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QQY0</accession>
    <name>A0A9F7QQY0_ICTPU</name>
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        <submittedName>
            <fullName evidence="10">E3 ubiquitin-protein ligase TRIM35-like</fullName>
        </submittedName>
    </protein>
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        <name evidence="10" type="primary">LOC128629032</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
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    </reference>
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    <dbReference type="KEGG" id="ipu:128629032"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12893">
        <property type="entry name" value="SPRY_PRY_TRIM35"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="12"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="84"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="8">
        <location>
            <begin position="272"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="194"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053531080.1"/>
        </source>
    </evidence>
    <sequence length="470" mass="54206" checksum="FE5EC1664CA93C33" modified="2023-06-28" version="1">MASKFSEEEFSCPVCCEIFTDPVLLRCSHSVCKVCLQKFWENKGSRECPVCRRKSSMEDPPICLALKNLCETFLQERSQRSSSASETVCSLHSEKLKLFCLDDQQPVCWVCRDSKIHTNHKFCPIDEALTDCKEELKTALKPLQEKLKIFKDCKLHWSQTAEHIKIQAQHTEQQIKEEFEKLHQVLRDEEAARIAALREEEEQKSQMMKEKIEKLSRDISSLSDTIRGVEEEMRAEDVSFLQNYEATVRRAQCTLQHPEELSGTLIHVAKHLDNLKFRVWEKMQDAVQYTPVTLDPNTAHPKLIVSDDLTSVRVSGEKQKLPDNPERFDYRCCILGSEVFNSGTHCWDVEVGDCTVWYVGVMTESAQRKGKIFSRSGIWYVWYYGGKYGTCSTPQSPTLLSVTQNLQRIRVQLDWNRGKLSFSDPLTNTHIHTFTHTFTDKLLPFIRVTCNKSPVKILPLQCSVRVNQIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QVD7</accession>
    <name>A0A9F7QVD7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Synaptonemal complex protein 2-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">sycp2l</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SYCP2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053530680.1">
        <property type="nucleotide sequence ID" value="XM_053674705.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053530681.1">
        <property type="nucleotide sequence ID" value="XM_053674706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QVD7"/>
    <dbReference type="GeneID" id="108256207"/>
    <dbReference type="KEGG" id="ipu:108256207"/>
    <dbReference type="CTD" id="221711"/>
    <dbReference type="OrthoDB" id="10256849at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024835">
        <property type="entry name" value="SYCP2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041322">
        <property type="entry name" value="SYCP2_ARLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040560">
        <property type="entry name" value="SYCP2_SLD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15607:SF18">
        <property type="entry name" value="SYNAPTONEMAL COMPLEX PROTEIN 2-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15607">
        <property type="entry name" value="SYNAPTONEMAL COMPLEX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18581">
        <property type="entry name" value="SYCP2_ARLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18584">
        <property type="entry name" value="SYCP2_SLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Synaptonemal complex protein 2 armadillo-repeat-like" evidence="7">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Synaptonemal complex protein 2 Spt16M-like" evidence="8">
        <location>
            <begin position="178"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="740"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="744"/>
            <end position="757"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053530680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053530681.1"/>
        </source>
    </evidence>
    <sequence length="862" mass="97221" checksum="764D723E598E7BEB" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R0R4</accession>
    <name>A0A9F7R0R4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Myosin VIIAa</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">myo7aa</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053533860.1">
        <property type="nucleotide sequence ID" value="XM_053677885.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108263906"/>
    <dbReference type="KEGG" id="ipu:108263906"/>
    <dbReference type="CTD" id="252846"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045177">
        <property type="term" value="C:apical part of cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
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    <sequence length="2197" mass="253483" checksum="3DB516E9047F20EA" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R3Z4</accession>
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        <name evidence="4" type="primary">si:dkey-21a6.5</name>
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        <name type="scientific">Ictalurus punctatus</name>
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        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
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    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_053682440.1"/>
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        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
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        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
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    <dbReference type="InterPro" id="IPR001368">
        <property type="entry name" value="TNFR/NGFR_Cys_rich_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011641">
        <property type="entry name" value="Tyr-kin_ephrin_A/B_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46967">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN,N-TERMINAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46967:SF1">
        <property type="entry name" value="KERATIN-ASSOCIATED PROTEIN 16-1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07699">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00208">
        <property type="entry name" value="TNFR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="2">
        <location>
            <begin position="114"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="2">
        <location>
            <begin position="148"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="2">
        <location>
            <begin position="182"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="2">
        <location>
            <begin position="216"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053538415.1"/>
        </source>
    </evidence>
    <sequence length="360" mass="38797" checksum="B0A4D40AD4F9B6B9" modified="2023-06-28" version="1">MLHFIRKSRSQSLEEEWRGTESKMLEGAVRSQTTLTPALTATTVMENVTATTVSEIVTATSPIMLSTTTPGCSILNTSSCEACAPGTYYDNETLLCSCCPEPGLCIFPSGCRSCSPGFYQPLEGQQNCLPCTPGFYTNFTASPVCQACLPGSYTNESGSVSCQACAPGFYTSRQNATVCNPCPYGTFCNSSSCAQCQMCPAGSEALKTGSRQCSPCRPGMHKTSHQKICQICNIGFYQIHWGQENCDICPEDHYCPSPDVNPILCPSDAFCPEGSTAPGYCMETFLRKAGDTCELAPVTIALIVIGGGLFLLFMVLLVCRRRRDADSELSLSRAPLLRKDRPSCHVYGVPCDADPVYAGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R4I9</accession>
    <name>A0A9F7R4I9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitochondrial tRNA methylthiotransferase CDK5RAP1</fullName>
            <ecNumber evidence="8">2.8.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">CDK5 activator-binding protein C42</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">CDK5 regulatory subunit-associated protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">mt-tRNA-2-methylthio-N6-dimethylallyladenosine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">mt-tRNA-N6-(dimethylallyl)adenosine(37) methylthiotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">cdk5rap1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(6)-dimethylallyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-dimethylallyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:37067"/>
            <dbReference type="Rhea" id="RHEA-COMP:10375"/>
            <dbReference type="Rhea" id="RHEA-COMP:10376"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:74415"/>
            <dbReference type="ChEBI" id="CHEBI:74417"/>
            <dbReference type="EC" id="2.8.4.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:37068"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methylthiotransferase family. MiaB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.3" evidence="8"/>
    <dbReference type="RefSeq" id="XP_053529963.1">
        <property type="nucleotide sequence ID" value="XM_053673988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R4I9"/>
    <dbReference type="GeneID" id="108255030"/>
    <dbReference type="CTD" id="51654"/>
    <dbReference type="OrthoDB" id="190098at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035597">
        <property type="term" value="F:tRNA-2-methylthio-N(6)-dimethylallyladenosine(37) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051246">
        <property type="term" value="P:regulation of protein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12160:FF:000003">
        <property type="entry name" value="CDK5 regulatory subunit-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.30.20:FF:000003">
        <property type="entry name" value="CDK5 regulatory subunit-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="entry name" value="Methylthiotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01864">
        <property type="entry name" value="tRNA_metthiotr_MiaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006463">
        <property type="entry name" value="MiaB_methiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00089">
        <property type="entry name" value="MiaB/RimO family radical SAM methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43020">
        <property type="entry name" value="CDK5 REGULATORY SUBUNIT-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43020:SF2">
        <property type="entry name" value="MITOCHONDRIAL TRNA METHYLTHIOTRANSFERASE CDK5RAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00273">
        <property type="entry name" value="(dimethylallyl)adenosine_tRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00413">
        <property type="entry name" value="CDK5RAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01061">
        <property type="entry name" value="methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="MTTase N-terminal" evidence="16">
        <location>
            <begin position="90"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="17">
        <location>
            <begin position="232"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="15">
        <location>
            <begin position="503"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00077166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00078237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00078249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_053529963.1"/>
        </source>
    </evidence>
    <sequence length="587" mass="66029" checksum="F97886A68442BD10" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R8C4</accession>
    <name>A0A9F7R8C4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7 8">SH2 domain-containing protein 4B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7 8" type="primary">sh2d4ba</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">SDA103</strain>
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            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="6 7 8">Blood</tissue>
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        <source>
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<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
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            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
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            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
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        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
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    </feature>
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        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1459"/>
            <end position="1474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1532"/>
            <end position="1545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1552"/>
            <end position="1561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1605"/>
            <end position="1623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1625"/>
            <end position="1635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1650"/>
            <end position="1659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1798"/>
            <end position="1814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1822"/>
            <end position="1839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1859"/>
            <end position="1876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1880"/>
            <end position="1904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1908"/>
            <end position="1923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1924"/>
            <end position="1935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1952"/>
            <end position="1967"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_053533618.1"/>
        </source>
    </evidence>
    <sequence length="2044" mass="228998" checksum="AB44830A8F8CFB16" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7REW3</accession>
    <name>A0A9F7REW3_ICTPU</name>
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        <submittedName>
            <fullName evidence="8">Centrosomal protein of 83 kDa isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cep83</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053541674.1">
        <property type="nucleotide sequence ID" value="XM_053685699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7REW3"/>
    <dbReference type="GeneID" id="108275300"/>
    <dbReference type="CTD" id="51134"/>
    <dbReference type="OrthoDB" id="311279at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097539">
        <property type="term" value="C:ciliary transition fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051660">
        <property type="term" value="P:establishment of centrosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052116">
        <property type="entry name" value="Centro_Cilium_Assembly"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23170:SF2">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 83 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23170">
        <property type="entry name" value="NY-REN-58 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="717"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="66"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="241"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="689"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="721"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053541674.1"/>
        </source>
    </evidence>
    <sequence length="740" mass="87080" checksum="87F673D2EF769F76" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RKU0</accession>
    <name>A0A9F7RKU0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="20 27">Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN</fullName>
            <ecNumber evidence="6 27">3.1.3.16</ecNumber>
            <ecNumber evidence="5 27">3.1.3.48</ecNumber>
            <ecNumber evidence="4 27">3.1.3.67</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="27">Phosphatase and tensin homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="34" type="primary">ptena</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="33">SDA103</strain>
        </source>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="34">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="27">Dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also functions as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring of PtdIns(3,4,5)P3/phosphatidylinositol 3,4,5-trisphosphate, PtdIns(3,4)P2/phosphatidylinositol 3,4-diphosphate and PtdIns3P/phosphatidylinositol 3-phosphate with a preference for PtdIns(3,4,5)P3.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + H2O = 1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:43560"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83420"/>
            <dbReference type="ChEBI" id="CHEBI:83423"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:43561"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>1,2-dioctanoyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + H2O = 1,2-dioctanoyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:43552"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83416"/>
            <dbReference type="ChEBI" id="CHEBI:83419"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:43553"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>1D-myo-inositol 1,3,4,5,6-pentakisphosphate + H2O = 1D-myo-inositol 1,4,5,6-tetrakisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77143"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57627"/>
            <dbReference type="ChEBI" id="CHEBI:57733"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:77144"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>1D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77155"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57895"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:77156"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:20630"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:47005"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:10685"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:25017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="EC" id="3.1.3.67"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:25018"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="27">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="27">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="27">Nucleus</location>
            <location evidence="27">PML body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Postsynaptic density</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 27">Belongs to the PTEN phosphatase protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="6 27"/>
    <dbReference type="EC" id="3.1.3.48" evidence="5 27"/>
    <dbReference type="EC" id="3.1.3.67" evidence="4 27"/>
    <dbReference type="RefSeq" id="XP_053538691.1">
        <property type="nucleotide sequence ID" value="XM_053682716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RKU0"/>
    <dbReference type="GeneID" id="108269686"/>
    <dbReference type="KEGG" id="ipu:108269686"/>
    <dbReference type="CTD" id="794088"/>
    <dbReference type="OrthoDB" id="16692at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051717">
        <property type="term" value="F:inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016314">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051800">
        <property type="term" value="F:phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048870">
        <property type="term" value="P:cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010648">
        <property type="term" value="P:negative regulation of cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051129">
        <property type="term" value="P:negative regulation of cellular component organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051093">
        <property type="term" value="P:negative regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051241">
        <property type="term" value="P:negative regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023057">
        <property type="term" value="P:negative regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043491">
        <property type="term" value="P:phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051896">
        <property type="term" value="P:regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14509">
        <property type="entry name" value="PTP_PTEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1110:FF:000003">
        <property type="entry name" value="Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000029">
        <property type="entry name" value="Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017361">
        <property type="entry name" value="Bifunc_PIno_P3_Pase/Pase_PTEN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051281">
        <property type="entry name" value="Dual-spec_lipid-protein_phosph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045101">
        <property type="entry name" value="PTP_PTEN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014020">
        <property type="entry name" value="Tensin_C2-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029023">
        <property type="entry name" value="Tensin_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305">
        <property type="entry name" value="PHOSPHATASE WITH HOMOLOGY TO TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305:SF81">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE 3-PHOSPHATASE AND DUAL-SPECIFICITY PROTEIN PHOSPHATASE PTEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10409">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22785">
        <property type="entry name" value="Tc-R-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038025">
        <property type="entry name" value="PTEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01326">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01301">
        <property type="entry name" value="PTPlike_phytase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51182">
        <property type="entry name" value="C2_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51181">
        <property type="entry name" value="PPASE_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7 27" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 27" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 27" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 27" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="15 27" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12 27" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0770">Synapse</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Phosphatase tensin-type" evidence="31">
        <location>
            <begin position="13"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="30">
        <location>
            <begin position="101"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="C2 tensin-type" evidence="32">
        <location>
            <begin position="189"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="284"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="380"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="29">
        <location>
            <begin position="301"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="29">
        <location>
            <begin position="311"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="29">
        <location>
            <begin position="381"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="29">
        <location>
            <begin position="410"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="28">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00034268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00034338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00043734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00043760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00043762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00051341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRNR" id="PIRNR038025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PIRSR" id="PIRSR038025-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS51182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="RefSeq" id="XP_053538691.1"/>
        </source>
    </evidence>
    <sequence length="430" mass="50414" checksum="89291AF3687FAFB2" modified="2023-06-28" version="1">MAFIKQIVSREKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDHWLSEDENHVAAIHCKAGKGRTGVMICAYLLHRGKFMEAQEALDFYGEVRTRDKKGVTIPSQRRYVHYYSFLLKNHLDYKPVALLFHKMVFETLPMFSSGTCNPQFVVYQLKVKIHTSNPSHTRREDKLIIFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPDESGERVENGSAMNDSDSHSSTQSQSSPSERDRGMGGGERERDRERDRERDRERDGERDYLILMLTKNDLDKANKDKANRYFSPNFKVKLFFTKTVDETSNSEASTSTSITPDVSDNEPDHYRYSDTTDSDPENEPFDEEQHTQITKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RLV3</accession>
    <name>A0A9F7RLV3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Vitellogenin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC128628790</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Precursor of the major egg-yolk proteins that are sources of nutrients during early development of oviparous organisms.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053539336.1">
        <property type="nucleotide sequence ID" value="XM_053683361.1"/>
    </dbReference>
    <dbReference type="GeneID" id="128628790"/>
    <dbReference type="KEGG" id="ipu:128628790"/>
    <dbReference type="OrthoDB" id="5956066at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045735">
        <property type="term" value="F:nutrient reservoir activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071391">
        <property type="term" value="P:cellular response to estrogen stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032355">
        <property type="term" value="P:response to estradiol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.50.20:FF:000001">
        <property type="entry name" value="Vitellogenin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.20:FF:000002">
        <property type="entry name" value="Vitellogenin 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.80.10:FF:000001">
        <property type="entry name" value="Vitellogenin 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.230.10:FF:000002">
        <property type="entry name" value="Vitellogenin 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.230.10">
        <property type="entry name" value="Lipovitellin, beta-sheet shell regions, chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.80.10">
        <property type="entry name" value="Lipovitellin-phosvitin complex, chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.50.20">
        <property type="entry name" value="Lipovitellin. Chain A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.90.10">
        <property type="entry name" value="Vitellinogen, beta-sheet shell domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.20">
        <property type="entry name" value="Vitellinogen, superhelical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015819">
        <property type="entry name" value="Lipid_transp_b-sht_shell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011030">
        <property type="entry name" value="Lipovitellin_superhlx_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015816">
        <property type="entry name" value="Vitellinogen_b-sht_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015258">
        <property type="entry name" value="Vitellinogen_b-sht_shell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037088">
        <property type="entry name" value="Vitellinogen_b-sht_shell_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015255">
        <property type="entry name" value="Vitellinogen_open_b-sht"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015817">
        <property type="entry name" value="Vitellinogen_open_b-sht_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050733">
        <property type="entry name" value="Vitellogenin/Apolipophorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001747">
        <property type="entry name" value="Vitellogenin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23345">
        <property type="entry name" value="VITELLOGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23345:SF9">
        <property type="entry name" value="VITELLOGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09175">
        <property type="entry name" value="Vit_b-sht_shell"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09172">
        <property type="entry name" value="Vit_open_b-sht"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01347">
        <property type="entry name" value="Vitellogenin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01169">
        <property type="entry name" value="DUF1943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01170">
        <property type="entry name" value="DUF1944"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00638">
        <property type="entry name" value="LPD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56968">
        <property type="entry name" value="Lipovitellin-phosvitin complex, beta-sheet shell regions"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48431">
        <property type="entry name" value="Lipovitellin-phosvitin complex, superhelical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51211">
        <property type="entry name" value="VITELLOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0758">Storage protein</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039940083" evidence="9">
        <location>
            <begin position="16"/>
            <end position="1550"/>
        </location>
    </feature>
    <feature type="domain" description="Vitellogenin" evidence="10">
        <location>
            <begin position="24"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1058"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1058"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="162"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="204"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053539336.1"/>
        </source>
    </evidence>
    <sequence length="1550" mass="167751" checksum="875AEFF765E6A685" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RQX0</accession>
    <name>A0A9F7RQX0_ICTPU</name>
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        <submittedName>
            <fullName evidence="10">NLR family CARD domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC128634279</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053540702.1">
        <property type="nucleotide sequence ID" value="XM_053684727.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A9F7RQX0"/>
    <dbReference type="GeneID" id="128634279"/>
    <dbReference type="KEGG" id="ipu:128634279"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000210">
        <property type="entry name" value="Si:dkey-16p6.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="8">
        <location>
            <begin position="230"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="10"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="36"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053540702.1"/>
        </source>
    </evidence>
    <sequence length="733" mass="84215" checksum="70DE10644060CB1D" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RUC0</accession>
    <name>A0A9F7RUC0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein zgc:162608</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">zgc:162608</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053543372.1">
        <property type="nucleotide sequence ID" value="XM_053687397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RUC0"/>
    <dbReference type="GeneID" id="108277432"/>
    <dbReference type="KEGG" id="ipu:108277432"/>
    <dbReference type="OMA" id="NPVAEEM"/>
    <dbReference type="OrthoDB" id="8942424at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.20">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1230">
        <property type="entry name" value="Apolipoprotein A-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58113">
        <property type="entry name" value="Apolipoprotein A-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039885416" evidence="1">
        <location>
            <begin position="17"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053543372.1"/>
        </source>
    </evidence>
    <sequence length="312" mass="35051" checksum="3C2D6B59CCF1AD6F" modified="2023-06-28" version="1">MFRYVIFAVTLWITTAFPVSQDPASREGFWSIQDESANLAIDKKDGTKQSDGMWKSHVVSSDLYSPDSLNSMAADLRHKLSQESDRLRARLRQELLDLRQRLSPYPSHPQVTPTNVHELLSPFTKSLQLALESNTQQLCGHLMMTVQGVQSNGPSLYQDATEKISQAMDESHKRRTAAFEDFKTKAFEAVEEDKDSSRKDLWEEVTARLGQEICSFSLEAQGKIAALKVSLTTLLTSSEPLGAEMVSKVDQFCDGSSRQNHKFIADLVQQISRLEEKQSKGESSSHVNIDSIQEDFSTRLNALLQDIVHSIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TET6</accession>
    <name>A0A9F7TET6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">DNA polymerase epsilon catalytic subunit</fullName>
            <ecNumber evidence="16">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">pole</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">DNA polymerase II participates in chromosomal DNA replication.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21 22">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the DNA polymerase epsilon complex consisting of four subunits: the catalytic subunit POLE and the accessory subunits POLE2, POLE3 and POLE4. Interacts with RAD17 and TOPBP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="16"/>
    <dbReference type="RefSeq" id="XP_053530552.1">
        <property type="nucleotide sequence ID" value="XM_053674577.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108255721"/>
    <dbReference type="KEGG" id="ipu:108255721"/>
    <dbReference type="CTD" id="5426"/>
    <dbReference type="OrthoDB" id="10060449at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008622">
        <property type="term" value="C:epsilon DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006287">
        <property type="term" value="P:base-excision repair, gap-filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045004">
        <property type="term" value="P:DNA replication proofreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05779">
        <property type="entry name" value="DNA_polB_epsilon_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05535">
        <property type="entry name" value="POLBc_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000002">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000010">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1600.10:FF:000006">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013697">
        <property type="entry name" value="DNA_pol_e_suA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029703">
        <property type="entry name" value="POL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055191">
        <property type="entry name" value="POL2_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054475">
        <property type="entry name" value="Znf-DPOE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670:SF0">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08490">
        <property type="entry name" value="DUF1744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22634">
        <property type="entry name" value="POL2_thumb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22912">
        <property type="entry name" value="zf-DPOE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23250">
        <property type="entry name" value="zf_DPOE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01159">
        <property type="entry name" value="DUF1744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 16" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 16" id="KW-0235">DNA replication</keyword>
    <keyword evidence="13 16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 16" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="11 16" id="KW-0408">Iron</keyword>
    <keyword evidence="12 16" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 16" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase epsilon catalytic subunit A C-terminal" evidence="18">
        <location>
            <begin position="1525"/>
            <end position="1925"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1189"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1226"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1289"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1936"/>
            <end position="1975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1234"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1939"/>
            <end position="1951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1952"/>
            <end position="1975"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00062298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU365029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_053530552.1"/>
        </source>
    </evidence>
    <sequence length="2286" mass="262304" checksum="5D684F6497CE5BC3" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TH51</accession>
    <name>A0A9F7TH51_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Kinesin-like protein KIF15-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">kif15</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-12 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053535158.1">
        <property type="nucleotide sequence ID" value="XM_053679183.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108263075"/>
    <dbReference type="KEGG" id="ipu:108263075"/>
    <dbReference type="CTD" id="56992"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01373">
        <property type="entry name" value="KISc_KLP2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000034">
        <property type="entry name" value="Kinesin family member 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031794">
        <property type="entry name" value="HMMR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044986">
        <property type="entry name" value="KIF15/KIN-12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37739">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37739:SF8">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15908">
        <property type="entry name" value="HMMR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="13">
        <location>
            <begin position="25"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="686"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="370"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="718"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="905"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1035"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1179"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1281"/>
            <end position="1359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="109"/>
            <end position="116"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_053535158.1"/>
        </source>
    </evidence>
    <sequence length="1362" mass="155902" checksum="1BCFD52961F3699B" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TRM8</accession>
    <name>A0A9F7TRM8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Fibrillin-2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC108276642</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fibrillin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053542881.1">
        <property type="nucleotide sequence ID" value="XM_053686906.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108276642"/>
    <dbReference type="KEGG" id="ipu:108276642"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001527">
        <property type="term" value="C:microfibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000005">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3TDZ2</accession>
    <name>E3TDZ2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10 12">Krueppel-like factor 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">KLF6</name>
        <name evidence="12" type="synonym">klf6</name>
        <name evidence="12" type="synonym">klf6a</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GU588572">
        <property type="protein sequence ID" value="ADO28528.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187348.1">
        <property type="nucleotide sequence ID" value="NM_001200419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TDZ2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000001011"/>
    <dbReference type="GeneID" id="100528149"/>
    <dbReference type="KEGG" id="ipu:100528149"/>
    <dbReference type="CTD" id="280650"/>
    <dbReference type="GeneTree" id="ENSGT00940000158085"/>
    <dbReference type="OMA" id="HLWNAIT"/>
    <dbReference type="OrthoDB" id="4748970at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001974">
        <property type="term" value="P:blood vessel remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048565">
        <property type="term" value="P:digestive tract development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043542">
        <property type="term" value="P:endothelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043249">
        <property type="term" value="P:erythrocyte maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001889">
        <property type="term" value="P:liver development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021634">
        <property type="term" value="P:optic nerve formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035265">
        <property type="term" value="P:organ growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031016">
        <property type="term" value="P:pancreas development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000471">
        <property type="term" value="P:regulation of hematopoietic stem cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902033">
        <property type="term" value="P:regulation of hematopoietic stem cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd21586">
        <property type="entry name" value="KLF6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000021">
        <property type="entry name" value="Basic krueppel-like factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000018">
        <property type="entry name" value="Krueppel-like factor 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF50">
        <property type="entry name" value="KRUEPPEL-LIKE FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="201"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="231"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="261"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="90"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADO28528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="NP_001187348.1"/>
        </source>
    </evidence>
    <sequence length="284" mass="31737" checksum="E5FFA3EF135A592E" modified="2011-01-11" version="1">MDVLPMCSIFQELQIVHDTGYFSALPSLEEYWQQTCLELERYLQSEPYVSAAELKFDPQEDLWSKLILACGDGDKSEMDSNSNADLAHVKEEDAQDGPMSETRSVNSDASSEVSDSSEELSPTHIFSSNPLGPGHLASSIIATPPSSPEALQEPAIPQLWGSIHTDLHVAGKARHSGTAKGSDKLALPGGDASPDGRRRVHRCHFNGCRKVYTKSSHLKAHQRTHTGEKPYRCSWEGCEWRFARSDELTRHFRKHTGAKPFKCSHCDRCFSRSDHLALHMKRHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3TEA2</accession>
    <name>E3TEA2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein RACK1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Guanine nucleotide-binding protein subunit beta-2-like 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Receptor of activated protein kinase C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">GBLP</name>
        <name evidence="13" type="synonym">gblp</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Required for VANGL2 membrane localization, inhibits Wnt signaling and regulates cellular polarization and oriented cell division during gastrulation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat G protein beta family. Ribosomal protein RACK1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GU588682">
        <property type="protein sequence ID" value="ADO28638.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187435.1">
        <property type="nucleotide sequence ID" value="NM_001200506.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TEA2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029887"/>
    <dbReference type="GeneID" id="100528236"/>
    <dbReference type="KEGG" id="ipu:100528236"/>
    <dbReference type="CTD" id="100528236"/>
    <dbReference type="OMA" id="AQVPYCV"/>
    <dbReference type="OrthoDB" id="7875889at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045182">
        <property type="term" value="F:translation regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001252">
        <property type="entry name" value="Receptor of activated protein C kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045223">
        <property type="entry name" value="RACK1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19868">
        <property type="entry name" value="RECEPTOR FOR ACTIVATED PROTEIN KINASE C RACK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 10" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="11"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="59"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="101"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="144"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="188"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="287"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00083655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADO28638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="NP_001187435.1"/>
        </source>
    </evidence>
    <sequence length="317" mass="35004" checksum="6509ED99F73F1A68" modified="2011-01-11" version="1">MTEQMTLRGTLKGHSGWVTQIATTPQFPDMILSASRDKSIIMWKLTRDETNYGVPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSADNRQIVSGSRDKTIKLWNTLGVCKYTIQDEGHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGFLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDTINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELRQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLIRVWQVTIGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3TEA8</accession>
    <name>E3TEA8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">GPN-loop GTPase 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">GPN3</name>
        <name evidence="11" type="synonym">gpn3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Small GTPase required for proper localization of RNA polymerase II (RNAPII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="function">
        <text evidence="7">Small GTPase required for proper nuclear import of RNA polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Binds to RNA polymerase II (RNAPII).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="GU588688">
        <property type="protein sequence ID" value="ADO28644.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187440.1">
        <property type="nucleotide sequence ID" value="NM_001200511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TEA8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019584"/>
    <dbReference type="GeneID" id="100528241"/>
    <dbReference type="KEGG" id="ipu:100528241"/>
    <dbReference type="CTD" id="51184"/>
    <dbReference type="GeneTree" id="ENSGT00950000183172"/>
    <dbReference type="OMA" id="LYTHMTV"/>
    <dbReference type="OrthoDB" id="5839at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17872">
        <property type="entry name" value="GPN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000616">
        <property type="entry name" value="GPN-loop GTPase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030228">
        <property type="entry name" value="Gpn3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF7">
        <property type="entry name" value="GPN-LOOP GTPASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="259"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADO28644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="NP_001187440.1"/>
        </source>
    </evidence>
    <sequence length="285" mass="32804" checksum="EB8E0263B08F76E0" modified="2011-01-11" version="1">MPRYAQLVMGPAGSGKSTYCSTMIQHAEAINRSIQVINLDPAAEHFNYPVMADIRELIQVDDVMEDDSLRFGPNGGLVFCMEYFASNFDWLEESLGHVEDDYILFDCPGQIELYTHLPVMKHLVEQLQQWEFRVCGVFLVDSQFMVESFKFISGVMAALSAMVALEIPQVNIMTKMDLLSPKAKKEIEKYLDPDMYSMMEDSSATMRSKKFMKLTKAICGLIDDYSMVRFLPFDRTDEEGINIVLQHIDFSIQYGEDLEFKEPKEDDEEPGNQNYDEYFQDRVDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3TET0</accession>
    <name>E3TET0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cwc15-like</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="8">Protein CWC15 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CWC15</name>
        <name evidence="8" type="synonym">cwc15</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
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                <person name="Lee Y."/>
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                <person name="Peatman E."/>
                <person name="Abernathy J."/>
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                <person name="Liu S."/>
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                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
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                <person name="Liu Z."/>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
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                <person name="Zhou T."/>
                <person name="Zeng Q."/>
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                <person name="Gao S."/>
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                <person name="Koren S."/>
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                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
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                <person name="Duke M.V."/>
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                <person name="Abernathy J."/>
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                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">SDA103</strain>
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    <reference evidence="6" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <property type="molecule type" value="mRNA"/>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ADO29484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="NP_001316257.1"/>
        </source>
    </evidence>
    <sequence length="222" mass="24806" checksum="57C2E174243E2F20" modified="2011-01-11" version="1">MDKKSESDSIDDGVSEAESCSASCLGATGGSDSRVDSQLADAVRLKTEGNAFYREKNVRSAIGRYHRALLVLRGLDSEVTSSLKAFRPQSPTLTPEQEELLRSTQIDCYNNLAACLLQREVVDYARVQEYSLKVLQWRAGDIKALYRAGVATLELGDAQTARHYLLQASRGKPNDANVKKQLQRVEERLSKDFEKEKALYKGMFNKQKQAEEDAVEKNPNQV</sequence>
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    <accession>Q1L607</accession>
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        <name type="common">Channel catfish</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
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            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2005-04" db="EMBL/GenBank/DDBJ databases">
            <title>Novel hypoxia-induced factors (HIF) and their inhibiting factor (FIH-1) from channel catfish (Ictalurus punctatus) and their expression under hypoxia.</title>
            <authorList>
                <person name="Wang Y."/>
                <person name="He C."/>
                <person name="Wang Q."/>
                <person name="Bao B."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Arias C."/>
                <person name="Liu Z."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
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    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DQ020169">
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    <dbReference type="RefSeq" id="NP_001187141.1">
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    <dbReference type="STRING" id="7998.ENSIPUP00000007127"/>
    <dbReference type="GeneID" id="100304653"/>
    <dbReference type="KEGG" id="ipu:100304653"/>
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    <dbReference type="OrthoDB" id="6021714at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021537">
        <property type="entry name" value="HIF_alpha-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043:SF34">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 SUBUNIT ALPHA,-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23171">
        <property type="entry name" value="bHLH_HIF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11413">
        <property type="entry name" value="HIF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14598">
        <property type="entry name" value="PAS_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
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    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BHLH" evidence="12">
        <location>
            <begin position="13"/>
            <end position="66"/>
        </location>
    </feature>
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            <begin position="91"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="11">
        <location>
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            <end position="290"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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            <end position="537"/>
        </location>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAZ75953.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="NP_001187141.1"/>
        </source>
    </evidence>
    <sequence length="627" mass="70704" checksum="584D22EC792AD6A8" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-02-19" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>V5N4L6</accession>
    <name>V5N4L6_ICTPU</name>
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        <submittedName>
            <fullName evidence="9">Hemoglobin alpha 1</fullName>
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        <submittedName>
            <fullName evidence="11">Hemoglobin, alpha adult 2</fullName>
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        <name evidence="11" type="primary">hbaa2</name>
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            <taxon>Actinopterygii</taxon>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2014" name="Comp. Biochem. Physiol. Part D Genomics Proteomics" volume="9" first="11" last="22">
            <title>Channel catfish hemoglobin genes: identification, phylogenetic and syntenic analysis, and specific induction in response to heat stress.</title>
            <authorList>
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                <person name="Liu S."/>
                <person name="Wang X."/>
                <person name="Wang R."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Li C."/>
                <person name="Kaltenboeck L."/>
                <person name="Li J."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="24334243"/>
            <dbReference type="DOI" id="10.1016/j.cbd.2013.11.002"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the globin family.</text>
    </comment>
    <dbReference type="EMBL" id="KF471101">
        <property type="protein sequence ID" value="AHA82583.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017309920.1">
        <property type="nucleotide sequence ID" value="XM_017454431.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="V5N4L6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000015406"/>
    <dbReference type="GeneID" id="108257035"/>
    <dbReference type="KEGG" id="ipu:108257035"/>
    <dbReference type="CTD" id="497166"/>
    <dbReference type="GeneTree" id="ENSGT00940000163288"/>
    <dbReference type="OrthoDB" id="8751793at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072562">
        <property type="term" value="C:blood microparticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031838">
        <property type="term" value="C:haptoglobin-hemoglobin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005833">
        <property type="term" value="C:hemoglobin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031720">
        <property type="term" value="F:haptoglobin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043177">
        <property type="term" value="F:organic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005344">
        <property type="term" value="F:oxygen carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08927">
        <property type="entry name" value="Hb-alpha-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.490.10:FF:000002">
        <property type="entry name" value="Hemoglobin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002338">
        <property type="entry name" value="Hemoglobin_a-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050056">
        <property type="entry name" value="Hemoglobin_oxygen_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002339">
        <property type="entry name" value="Hemoglobin_pi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11442:SF91">
        <property type="entry name" value="EMBRYONIC ALPHA GLOBIN E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11442">
        <property type="entry name" value="HEMOGLOBIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00612">
        <property type="entry name" value="ALPHAHAEM"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00815">
        <property type="entry name" value="PIHAEM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3 7" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0561">Oxygen transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Globin" evidence="8">
        <location>
            <begin position="2"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AHA82583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017309920.1"/>
        </source>
    </evidence>
    <sequence length="143" mass="15634" checksum="1AC9147B04BF94A2" modified="2014-02-19" version="1">MSLTDIDKTLVKAFWGKVSGKADAIGHEALVRMLLVYPQTKIYFSHWPDLSPGSESVKKHGRTVIGALNDAVVNIDDLVGGLSALSDLHAYKLRVEPGNFKILSHNFLVTLAINFPADFTPEVHIAMDKFLAAVSAALADKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5U994</accession>
    <name>W5U994_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13 14">Probable G-protein coupled receptor 146</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="11">Putative G-protein coupled receptor 146</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 13 14" type="primary">gpr146</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13 14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT409166">
        <property type="protein sequence ID" value="AHH38581.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017310516.1">
        <property type="nucleotide sequence ID" value="XM_017455027.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053531314.1">
        <property type="nucleotide sequence ID" value="XM_053675339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U994"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000028760"/>
    <dbReference type="GeneID" id="108257346"/>
    <dbReference type="KEGG" id="ipu:108257346"/>
    <dbReference type="CTD" id="115330"/>
    <dbReference type="OMA" id="YICSHVA"/>
    <dbReference type="OrthoDB" id="8660770at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14990">
        <property type="entry name" value="7tmA_GPR146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047143">
        <property type="entry name" value="GPER1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037487">
        <property type="entry name" value="GPR146"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24226:SF3">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 146-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24226">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 182 AND ESTROGEN RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="150"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="234"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="44"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AHH38581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017310516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_053531314.1"/>
        </source>
    </evidence>
    <sequence length="329" mass="37155" checksum="A6D67848CC02F5F2" modified="2014-04-16" version="1">MWSCMVYNETEGNTDLAVCWDLGLALSIFSFFYLIVCFPMGLCYNALLVAVNLSNKMSMTMPDVYFVNMAIAGLVLNLVAPVVLLGPDFTRWPAWEHNDEVYITLLILFNISSLVIMYSTALLSLDYYIERALPRTYMSSVYNTKHVCGFIWGGAVLTSFSSLLFYVCNHVSAKILECSKMQNKEAADAIMTLIGYAVPAAAVLYALCLILRIRKEATPLDQDSGRMDPSIHRLLLACVGMQFVLWTPYYTTLLLLTVIDVPRMQTTGTYAFLRGFSQLLAFSSSFAMPLMYRKMNKNFAHKLQRLLKRLHCGKQSCPHEHSVIQQVIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5U9I0</accession>
    <name>W5U9I0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kish</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 12" type="primary">tmem167b</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Involved in the early part of the secretory pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the KISH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="JT408033">
        <property type="protein sequence ID" value="AHH38214.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017305612.1">
        <property type="nucleotide sequence ID" value="XM_017450123.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U9I0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035728"/>
    <dbReference type="GeneID" id="108254805"/>
    <dbReference type="KEGG" id="ipu:108254805"/>
    <dbReference type="CTD" id="56900"/>
    <dbReference type="OMA" id="IVMAFYI"/>
    <dbReference type="OrthoDB" id="10034655at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042863">
        <property type="entry name" value="Kish-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009653">
        <property type="entry name" value="Ksh1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46815">
        <property type="entry name" value="PROTEIN KISH-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46815:SF1">
        <property type="entry name" value="PROTEIN KISH-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06842">
        <property type="entry name" value="DUF1242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="44"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU910717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AHH38214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017305612.1"/>
        </source>
    </evidence>
    <sequence length="74" mass="8400" checksum="ACC8ECB15DABF2DD" modified="2014-04-16" version="1">MTNVYSFDGIVVFGLLFICTCAYLKKVPRLNSWLLSEKKGVWGVFYKAAIIGTRLHIAVALSCLFMAFYLVFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>W5UBK1</accession>
    <accession>A0A2D0RYK6</accession>
    <name>W5UBK1_ICTPU</name>
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            <fullName evidence="1">Peroxisomal membrane protein 4</fullName>
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    </protein>
    <gene>
        <name evidence="2" type="primary">PXMP4</name>
        <name evidence="4" type="synonym">pxmp4</name>
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    <organism evidence="2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Interacts with PEX19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT411137">
        <property type="protein sequence ID" value="AHH39318.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JT418489">
        <property type="protein sequence ID" value="AHH42754.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017335579.2">
        <property type="nucleotide sequence ID" value="XM_017480090.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UBK1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029278"/>
    <dbReference type="GeneID" id="108272024"/>
    <dbReference type="KEGG" id="ipu:108272024"/>
    <dbReference type="CTD" id="11264"/>
    <dbReference type="GeneTree" id="ENSGT00390000001562"/>
    <dbReference type="OrthoDB" id="39659at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019531">
        <property type="entry name" value="Pmp4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15460">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15460:SF3">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF013674">
        <property type="entry name" value="PXMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR013674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AHH39318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017335579.2"/>
        </source>
    </evidence>
    <sequence length="210" mass="23885" checksum="9BA997EF84FA5E6E" modified="2014-04-16" version="1">MAGSEVLKTLLYTINTLLQQEKYKAALAVLKGFRNGAVYGAKIRAPHALVMTFLFRSGSLQSKLKAIAQATYTHSRNLAYFVFSYKGLLALQKYIQGKSLQSHSFLAACLGGWLVFGDNNHINSQINMYLLSRILFALCQLAVEKGYIPKPKSDPFPLFAMLVWGIVLWLFEYHPHTLQPSLQSSMNYLYHDSNTWHDLSDFLIYNKPRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5UCA0</accession>
    <name>W5UCA0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">START domain-containing protein 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">PCTP-like protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">StAR-related lipid transfer protein 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">Stard10</name>
        <name evidence="21" type="synonym">stard14</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
            <location evidence="1">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT405716">
        <property type="protein sequence ID" value="AHH37292.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017329705.1">
        <property type="nucleotide sequence ID" value="XM_017474216.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UCA0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000028705"/>
    <dbReference type="GeneID" id="108268887"/>
    <dbReference type="KEGG" id="ipu:108268887"/>
    <dbReference type="CTD" id="100001416"/>
    <dbReference type="GeneTree" id="ENSGT00940000165821"/>
    <dbReference type="OMA" id="NTGPNSC"/>
    <dbReference type="OrthoDB" id="5403181at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08871">
        <property type="entry name" value="START_STARD10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000008">
        <property type="entry name" value="START domain containing 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041951">
        <property type="entry name" value="STARD10_START"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051213">
        <property type="entry name" value="START_lipid_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308:SF33">
        <property type="entry name" value="START DOMAIN CONTAINING 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0282">Flagellum</keyword>
    <keyword evidence="9" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="11" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="START" evidence="18">
        <location>
            <begin position="37"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="249"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00070345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00076937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00080073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AHH37292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017329705.1"/>
        </source>
    </evidence>
    <sequence length="272" mass="30960" checksum="13384CEF6AD6723F" modified="2014-04-16" version="1">MSLGSEMIRGEALFSDFRRQCSATENWQNKYNKNGMEVWVEVPPVNNSPQGNRNIYSKVHKVKCKIAINDVSAATIYDVLHDNIYRKTWDPVMLESFEIARLGPNSDVGYYSWLCPKPLKNRDVVTVRSWHASEDEYIIVNYSVKHPKYPPRKDLVRAVSILTGYLVKPTGPNSCSFIYLSQADPKGSLPKWAVNKATQVLAPRVMKSVYKAGQNYTVWKAQNSPEHKPWLYPIQSELPMMDPAELSIQRGDSLENVDETSTRDAVENEDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5UCT8</accession>
    <name>W5UCT8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">F-box only protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">fbxo9</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins and acts as a regulator of mTOR signaling.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXO9).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT412360">
        <property type="protein sequence ID" value="AHH39816.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017320071.1">
        <property type="nucleotide sequence ID" value="XM_017464582.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UCT8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000023166"/>
    <dbReference type="GeneID" id="108263595"/>
    <dbReference type="KEGG" id="ipu:108263595"/>
    <dbReference type="CTD" id="26268"/>
    <dbReference type="GeneTree" id="ENSGT00390000014256"/>
    <dbReference type="OMA" id="RWNRLDF"/>
    <dbReference type="OrthoDB" id="2117972at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22089">
        <property type="entry name" value="F-box_FBXO9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000012">
        <property type="entry name" value="F-box only protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045464">
        <property type="entry name" value="Hrt3/FBXO9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12874">
        <property type="entry name" value="F-BOX ONLY PROTEIN 48-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12874:SF29">
        <property type="entry name" value="F-BOX ONLY PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19270">
        <property type="entry name" value="FBO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="8">
        <location>
            <begin position="160"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AHH39816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017320071.1"/>
        </source>
    </evidence>
    <sequence length="423" mass="49445" checksum="464CD5D4991CD383" modified="2014-04-16" version="1">MDENNQNADVGSVEEGEDENSEDSNLQLELSAFRAQWMSELRPGSGYKTGLSKAADLKKTQELAREEKARELFLKAVEEEQNGAVYEAIKYYKSAMQLVPDIEFKINYSRSPDPDRVGGSYLEENKNDGEIDDLLAYLQQQLTVEHSSQKICQPEVETSQVHISALPLEVLMYIFRWVVSSDLDMRALEQLSLVCRGFYICARDPEIWRSACLRVWGRSCTKSLPFTSWREMFLERPRVRYDGVYISKTSYIRQGEESLDGFYRAWHQVDYYRYLRFFPDGHVMMLTTPEEPLVTLPRLRSKSTRMESIMIGHYRLSQDTDNQTKVYVVVSKRKEEKVPEYQRRRFCRQYPAPETERSFHVGLQLSSGGRQRFNKLVWIHHSCHITYRSTGETVITAFDLDKMYTPLFFARVKSYTAFSERPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5UCX0</accession>
    <name>W5UCX0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Serine incorporator 1</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="10">Serine incorporator 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Serinc1</name>
        <name evidence="10" type="synonym">serinc2l</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TDE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT406349">
        <property type="protein sequence ID" value="AHH37557.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017331411.1">
        <property type="nucleotide sequence ID" value="XM_017475922.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UCX0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000024116"/>
    <dbReference type="GeneID" id="108269842"/>
    <dbReference type="KEGG" id="ipu:108269842"/>
    <dbReference type="CTD" id="100329297"/>
    <dbReference type="GeneTree" id="ENSGT01030000234623"/>
    <dbReference type="OMA" id="KLSWINT"/>
    <dbReference type="OrthoDB" id="5963193at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005016">
        <property type="entry name" value="TDE1/TMS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383">
        <property type="entry name" value="SERINE INCORPORATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383:SF64">
        <property type="entry name" value="SERINE INCORPORATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03348">
        <property type="entry name" value="Serinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540155" evidence="7">
        <location>
            <begin position="17"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="198"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="384"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="426"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AHH37557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017331411.1"/>
        </source>
    </evidence>
    <sequence length="456" mass="50803" checksum="59BE443303392E00" modified="2014-04-16" version="1">MGACMALCSLASCASCLCGSAPCILCGCCPSSNNSTVTRLIFSFFLLLGTLVSIIMILPGMETQLRKIPGFCQGGSTIPGLENQFNCDVIVGYKSVYRMCFAMACFFFLFAAIMIRVRSSKDPRAAIQNGFWFFKFLILVGITVGAFFIPDGTFHNVWFYFGIVGSFMFILIQLILLIDFAHSWNEVWVRNAEEGSKGWFFGLLFFTILHYVLAFAAVVLFYLYYTKPDSCTEHKVFISLNLIFCVIISVVSILPKVQNASPQSGLLQSSVISLYTMYVTWSAMTNNPNRECNPSLLSLVSNVSVSTTQSPPTSAPGIVQWWDAQGIVGLVIFLFCTFYASIRSSSNAQVNRLMQTEEGHGSDVEGPVGEDGLRRVVDNEEDGVTYSYSFFHFHLFLASLYIMMTLTNWYKPDITTQAMQSSMPAVWVKICSSWLGLALYLWTLLAPLILPNRDFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5UEG1</accession>
    <name>W5UEG1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vesicle transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SFT2D2</name>
        <name evidence="12" type="synonym">sft2d2a</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">May be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the SFT2 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT407724">
        <property type="protein sequence ID" value="AHH38097.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053539611.1">
        <property type="nucleotide sequence ID" value="XM_053683636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UEG1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033691"/>
    <dbReference type="KEGG" id="ipu:108271516"/>
    <dbReference type="GeneTree" id="ENSGT00390000018525"/>
    <dbReference type="OMA" id="CVREAIW"/>
    <dbReference type="OrthoDB" id="73614at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011691">
        <property type="entry name" value="Vesicle_transpt_SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137:SF1">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN SFT2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AHH38097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053539611.1"/>
        </source>
    </evidence>
    <sequence length="171" mass="19069" checksum="ADC602DDAD8742E9" modified="2014-04-16" version="1">MDKLRAVLSGRDGDTDSTPNVIEAVNEASTLSWRTRVRGFAVCMGIGIFCSILGVCCLFLPKIGLILFIVFYTLGNICSLASTLFLMGPVKQLKRMFDKTRVVATVVMIVCLVLTLCAAFWWKIFALTLLFVILQAIAFAWYGLSYIPFARDAILKFFSSMCKMCIKCQCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5UGR2</accession>
    <name>W5UGR2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8 10 11">Homeobox protein PKNOX1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PKNOX1</name>
        <name evidence="10 11" type="synonym">pknox1.1</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TALE/MEIS homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="JT409865">
        <property type="protein sequence ID" value="AHH38852.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017325813.1">
        <property type="nucleotide sequence ID" value="XM_017470324.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017325814.1">
        <property type="nucleotide sequence ID" value="XM_017470325.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UGR2"/>
    <dbReference type="GeneID" id="108266681"/>
    <dbReference type="CTD" id="798151"/>
    <dbReference type="OrthoDB" id="10056939at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000004">
        <property type="entry name" value="Meis2 homeobox isoform 2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032453">
        <property type="entry name" value="PKNOX/Meis_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16493">
        <property type="entry name" value="Meis_PKNOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2 5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="7">
        <location>
            <begin position="251"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="253"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="389"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="393"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="405"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AHH38852.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017325813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017325814.1"/>
        </source>
    </evidence>
    <sequence length="420" mass="46889" checksum="F244A869D2977B8F" modified="2014-04-16" version="1">MMAGQPVSVHKYEEGVQEEVQQTDGDPEKSFSDTGMGRICNNALAESTRMETDKASIYRHPLFPLLALLFEKCEQSTQSSECITSESFDIDIQNFVLSQEKETKAFFSEDSELDSLMVKAIQVLRIHLLELEKVNDLCKDFCSRYISCLMSKMNSETLLSGETDDPYSHLHTQGIHGTVVPASPMQQGNISAVSQSQMVSGGAMYQPVTVVTPQGHVMAQTISPSKIHIQNSQHLQLQMNQDFGFFTSEDSSSKNKRGVLPKQATNVMRSWLFQHIAHPYPTEDEKKQIAHQTNLTLLQVNNWFINARRRILQPMLDASATEMPKTKKKTPQARPLQRFWPDSIASSVSQQQFTMEEVNIVTVGMSMDGYQTLSSDGATLAMQQVMMGNHSEDETDNSEDEGDTHLSSASMTDLGLNASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5UHA8</accession>
    <name>W5UHA8_ICTPU</name>
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        <submittedName>
            <fullName evidence="12 14">RING finger protein 150</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">rnf150</name>
        <name evidence="14" type="synonym">rnf150b</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT410443">
        <property type="protein sequence ID" value="AHH39062.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017342294.1">
        <property type="nucleotide sequence ID" value="XM_017486805.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UHA8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003258"/>
    <dbReference type="GeneID" id="108275805"/>
    <dbReference type="KEGG" id="ipu:108275805"/>
    <dbReference type="CTD" id="792211"/>
    <dbReference type="OMA" id="HERRACD"/>
    <dbReference type="OrthoDB" id="5357315at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02122">
        <property type="entry name" value="PA_GRAIL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16805">
        <property type="entry name" value="RING-H2_RNF150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000003">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000009">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ATL42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539:SF27">
        <property type="entry name" value="RING FINGER PROTEIN 128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540274" evidence="10">
        <location>
            <begin position="27"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="198"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="267"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AHH39062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017342294.1"/>
        </source>
    </evidence>
    <sequence length="424" mass="46778" checksum="D9814FD58AF897FB" modified="2014-04-16" version="1">MWRSVTEACRGLALSVWLVSLCSVRAPCLDFAAEKEEWYTAFVSVTYVDPDTAQLRTEKSECGHYGEHSPKRDAKGLLATAASARERHACDPNTRFAVPDRAGSWIALVTHGNCTFRDKIRHASGQNASAVVIFNVGASNANETITMPHPGTGDVVAIMIPEHKGRELVLLLERNVTVNMHITIGTRNLQKYVSRTSVVFVSISFIVLMIISLAWLVFYYIQRFRYANARDRNQRRLGDAAKKAISQLQVRTIRKGDQETESDFDNCAVCIEGYKPNDVVRILPCRHVFHKGCVDPWLVDHRTCPMCKMNILKALGLMSSAECLDELPLDYDLAVGGVALNAMVMSDDVLAGDITGGRDPGVRMVGLPQVLLDSEPITEDTLLTEQSELQPIVSSASEASLAEGAGQYDMDADSMECHLSSRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5UI90</accession>
    <name>W5UI90_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7 9">Surfeit locus protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">surf6</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SURF6 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT411310">
        <property type="protein sequence ID" value="AHH39377.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017315205.1">
        <property type="nucleotide sequence ID" value="XM_017459716.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UI90"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000011858"/>
    <dbReference type="GeneID" id="108259913"/>
    <dbReference type="KEGG" id="ipu:108259913"/>
    <dbReference type="CTD" id="6838"/>
    <dbReference type="GeneTree" id="ENSGT00390000006980"/>
    <dbReference type="OMA" id="QKKRTDN"/>
    <dbReference type="OrthoDB" id="444809at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029190">
        <property type="entry name" value="Rrp14/SURF6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007019">
        <property type="entry name" value="SURF6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14369">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14369:SF0">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04935">
        <property type="entry name" value="SURF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal RNA-processing protein 14/surfeit locus protein 6 C-terminal" evidence="6">
        <location>
            <begin position="134"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="17"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="297"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="237"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="184"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="297"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="324"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="351"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AHH39377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017315205.1"/>
        </source>
    </evidence>
    <sequence length="361" mass="42690" checksum="4B97FA3B494A5D09" modified="2014-04-16" version="1">MSSLVAKYEYLEKFTRKRWHSEDQKPKKRGYVPFQGRSDCEAGPHKKKQKNNWEPGKGRNMNTSYRKPTPPSIHKPAQNAVQVKVQRGKRVKMQQKTEGTKSEEFNAVDILKKRLHEKIEASRAQGTPKDPSSEEVQKRRAKRKQERERKKRKRKEFRMKKLAESASIEVKVENETEPAPVPLSDSTGSKPAKMDTNTIVFNKVEVGEEYVDKATQLKEKKKRKAKRGLTPLTGRNVKQLLSRVEARKARLEELREKDEKKAKKEEEKMRWTNVLYKAEGLKIKDNEDLLRASLKRKEKMKTRRKKKWAERSQQVLEKMQQRQDKRRKNIQKFKQNKMEKRKQRIRKKGRVLPEDLKKAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>W5UIK1</accession>
    <accession>A0A2D0QCF0</accession>
    <name>W5UIK1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">NADPH-dependent diflavin oxidoreductase 1</fullName>
            <ecNumber evidence="12">1.18.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">NADPH-dependent FMN and FAD-containing oxidoreductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15 17" type="primary">ndor1</name>
        <name evidence="12" type="synonym">NDOR1</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2023-07" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">NADPH-dependent reductase which is a central component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Transfers electrons from NADPH via its FAD and FMN prosthetic groups to the [2Fe-2S] cluster of CIAPIN1, another key component of the CIA machinery. In turn, this reduced cluster provides electrons for assembly of cytosolic iron-sulfur cluster proteins. It can also reduce the [2Fe-2S] cluster of CISD1 and activate this protein implicated in Fe/S cluster repair. In vitro can fully activate methionine synthase/MTR in the presence of soluble cytochrome b5/CYB5A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>2 oxidized [2Fe-2S]-[protein] + NADPH = 2 reduced [2Fe-2S]-[protein] + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67716"/>
            <dbReference type="Rhea" id="RHEA-COMP:17327"/>
            <dbReference type="Rhea" id="RHEA-COMP:17328"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:67717"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with CIAPIN1; as part of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Interacts with DCPS.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
            <location evidence="12">Perinuclear region</location>
        </subcellularLocation>
        <text evidence="12">Concentrated in perinuclear structure.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the NADPH-dependent diflavin oxidoreductase NDOR1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">In the N-terminal section; belongs to the flavodoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.18.1.-" evidence="12"/>
    <dbReference type="EMBL" id="JT416183">
        <property type="protein sequence ID" value="AHH41964.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017315977.2">
        <property type="nucleotide sequence ID" value="XM_017460488.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UIK1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000010310"/>
    <dbReference type="GeneID" id="108260312"/>
    <dbReference type="KEGG" id="ipu:108260312"/>
    <dbReference type="CTD" id="27158"/>
    <dbReference type="OrthoDB" id="1856718at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160246">
        <property type="term" value="F:NADPH-iron-sulfur [2Fe-2S] protein oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.990.10:FF:000008">
        <property type="entry name" value="NADPH-dependent diflavin oxidoreductase 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NADPH-dependent diflavin oxidoreductase 1"/>
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        <property type="entry name" value="NADPH-dependent diflavin oxidoreductase 1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03178">
        <property type="entry name" value="NDOR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
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    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
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    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
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        <property type="entry name" value="NDOR1"/>
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        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
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        <property type="entry name" value="NADPH-DEPENDENT DIFLAVIN OXIDOREDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
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        <property type="entry name" value="FPNCR"/>
    </dbReference>
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        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 12" id="KW-0274">FAD</keyword>
    <keyword evidence="4 12" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 12" id="KW-0288">FMN</keyword>
    <keyword evidence="7 12" id="KW-0521">NADP</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="13">
        <location>
            <begin position="6"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="14">
        <location>
            <begin position="204"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="59"/>
            <end position="62"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="381"/>
            <end position="384"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="415"/>
            <end position="418"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="459"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="514"/>
            <end position="515"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="520"/>
            <end position="524"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="595"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00059862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00063044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AHH41964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017315977.2"/>
        </source>
    </evidence>
    <sequence length="596" mass="67164" checksum="D7BD589F00408268" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5UJE1</accession>
    <name>W5UJE1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Iduronate 2-sulfatase</fullName>
            <ecNumber evidence="15">3.1.6.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Alpha-L-iduronate sulfate sulfatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">Ids</name>
        <name evidence="22" type="synonym">ids</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Lysosomal enzyme involved in the degradation pathway of dermatan sulfate and heparan sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Hydrolysis of the 2-sulfate groups of the L-iduronate 2-sulfate units of dermatan sulfate, heparan sulfate and heparin.</text>
            <dbReference type="EC" id="3.1.6.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">Monomer. The 58-kDa mature form is composed of two chains resulting from proteolitic processing, the 42-kDa chain and the 14-kDa chain that remain stably associated and form the 58-kDa intermediate form which is enzymatically active.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sulfatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.6.13" evidence="15"/>
    <dbReference type="EMBL" id="JT417133">
        <property type="protein sequence ID" value="AHH42269.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017329507.1">
        <property type="nucleotide sequence ID" value="XM_017474018.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UJE1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021624"/>
    <dbReference type="GeneID" id="108268807"/>
    <dbReference type="KEGG" id="ipu:108268807"/>
    <dbReference type="CTD" id="3423"/>
    <dbReference type="OrthoDB" id="96314at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043202">
        <property type="term" value="C:lysosomal lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004423">
        <property type="term" value="F:iduronate-2-sulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901136">
        <property type="term" value="P:carbohydrate derivative catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16030">
        <property type="entry name" value="iduronate-2-sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000027">
        <property type="entry name" value="iduronate 2-sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035874">
        <property type="entry name" value="IDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45953">
        <property type="entry name" value="IDURONATE 2-SULFATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45953:SF1">
        <property type="entry name" value="IDURONATE 2-SULFATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00149">
        <property type="entry name" value="SULFATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0458">Lysosome</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 18" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540313" description="Iduronate 2-sulfatase" evidence="18">
        <location>
            <begin position="26"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase N-terminal" evidence="19">
        <location>
            <begin position="28"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00062513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00066413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00068336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="AHH42269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017329507.1"/>
        </source>
    </evidence>
    <sequence length="563" mass="63707" checksum="ECFCDE6390EC02C4" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5UK01</accession>
    <name>W5UK01_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">carnitine O-palmitoyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">CPT1A</name>
        <name evidence="22 23 24" type="synonym">cpt1ab</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22 23 24" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22 23 24">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(R)-carnitine + hexadecanoyl-CoA = O-hexadecanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:12661"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:17490"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="EC" id="2.3.1.21"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:12662"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.21" evidence="4"/>
    <dbReference type="EMBL" id="JT416334">
        <property type="protein sequence ID" value="AHH42015.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017341222.1">
        <property type="nucleotide sequence ID" value="XM_017485733.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017341223.1">
        <property type="nucleotide sequence ID" value="XM_017485734.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017341224.1">
        <property type="nucleotide sequence ID" value="XM_017485735.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UK01"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007678"/>
    <dbReference type="GeneID" id="108275128"/>
    <dbReference type="KEGG" id="ipu:108275128"/>
    <dbReference type="CTD" id="560000"/>
    <dbReference type="OMA" id="KMDGTPT"/>
    <dbReference type="OrthoDB" id="240216at2759"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004095">
        <property type="term" value="F:carnitine O-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009437">
        <property type="term" value="P:carnitine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.70:FF:000001">
        <property type="entry name" value="Carnitine O-palmitoyltransferase 1, liver isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000042">
        <property type="entry name" value="Carnitine Palmitoyl Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032476">
        <property type="entry name" value="CPT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF55">
        <property type="entry name" value="CARNITINE O-PALMITOYLTRANSFERASE 1, BRAIN ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16484">
        <property type="entry name" value="CPT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00439">
        <property type="entry name" value="ACYLTRANSF_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00440">
        <property type="entry name" value="ACYLTRANSF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="13 16" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Carnitine O-palmitoyltransferase N-terminal" evidence="19">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="18">
        <location>
            <begin position="175"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF16484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="AHH42015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017341222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017341223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_017341224.1"/>
        </source>
    </evidence>
    <sequence length="774" mass="88605" checksum="27158B2F70B77C76" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5ULH3</accession>
    <name>W5ULH3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphorylase kinase</fullName>
            <ecNumber evidence="3">2.7.11.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">PHKG2</name>
        <name evidence="18 19" type="synonym">phkg2</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18 19" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18 19">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 ATP + phosphorylase b = 2 ADP + phosphorylase a.</text>
            <dbReference type="EC" id="2.7.11.19"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.19" evidence="3"/>
    <dbReference type="EMBL" id="JT418765">
        <property type="protein sequence ID" value="AHH42836.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017338566.1">
        <property type="nucleotide sequence ID" value="XM_017483077.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017338567.1">
        <property type="nucleotide sequence ID" value="XM_017483078.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ULH3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026814"/>
    <dbReference type="GeneID" id="108273676"/>
    <dbReference type="KEGG" id="ipu:108273676"/>
    <dbReference type="CTD" id="5261"/>
    <dbReference type="OMA" id="FPVMGPE"/>
    <dbReference type="OrthoDB" id="419455at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005964">
        <property type="term" value="C:phosphorylase kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004689">
        <property type="term" value="F:phosphorylase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000138">
        <property type="entry name" value="Phosphorylase b kinase gamma catalytic chain, liver/testis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000149">
        <property type="entry name" value="phosphorylase b kinase gamma catalytic chain, liver/testis isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002291">
        <property type="entry name" value="Phosph_kin_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01049">
        <property type="entry name" value="PHOSPHBKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="8 16 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="24"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AHH42836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017338566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017338567.1"/>
        </source>
    </evidence>
    <sequence length="390" mass="45139" checksum="739876CD870C3FEC" modified="2014-04-16" version="1">MTRDLMLGVELPHWVGAKEFHQKYESKEVIGRGVSSVVRRCVNKHTGQEFAVKIIEITAEKMTAQQLEEVKSSTLKEIHVLNVLKGHPSIITLIDSYESATFIFLVFDLMRRGELFDYLTEKVTLSEKETRNMMRALLEAVQYLHSLNIVHRDLKPENILLDDQGHIKLSDFGFSVQLQPGEKLRELCGTPGYLAPEILKCSMDETHEGYGKEVDLWACGVILFTLLAGSPPFWHRKQMLMLRMIMEGQYQFSSPEWDDRSDTVKDLISRLLVLDPAVRLTAEQALTHPFFLQYQKEDVRLFSPRKTFRVLILTVLSCIRMYCRYNRARPLTRELLMRDPYSLRAVRKLIDGCAFRIYGHWVKKGEQQNRAALFQNTTKIVLLGLEDLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5UM56</accession>
    <name>W5UM56_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ubiquitin-related modifier</fullName>
            <shortName evidence="7">SUMO</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SUMO3</name>
        <name evidence="11" type="synonym">sumo3a</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex sae1-sae2 and linkage to the E2 enzyme ube2i.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with SAE2 and UBE2I. Covalently attached to a number of proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">PML body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ubiquitin family. SUMO subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="JT419378">
        <property type="protein sequence ID" value="AHH43056.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017337511.1">
        <property type="nucleotide sequence ID" value="XM_017482022.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UM56"/>
    <dbReference type="SMR" id="W5UM56"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027451"/>
    <dbReference type="GeneID" id="108273059"/>
    <dbReference type="KEGG" id="ipu:108273059"/>
    <dbReference type="CTD" id="406398"/>
    <dbReference type="OMA" id="SKMMNAY"/>
    <dbReference type="OrthoDB" id="442921at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd16115">
        <property type="entry name" value="Ubl_SUMO2_3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000022">
        <property type="entry name" value="Small ubiquitin-related modifier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022617">
        <property type="entry name" value="Rad60/SUMO-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10562">
        <property type="entry name" value="SMALL UBIQUITIN-RELATED MODIFIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11976">
        <property type="entry name" value="Rad60-SLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="15"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00057717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AHH43056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017337511.1"/>
        </source>
    </evidence>
    <sequence length="94" mass="10786" checksum="DD2FCD3AB033A43F" modified="2014-04-16" version="1">MSEDKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGLSIRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGGHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5URI3</accession>
    <name>W5URI3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Ubiquitin associated and SH3 domain containing Bb</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="10">Ubiquitin-associated and SH3 domain-containing protein B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">UBASH3B</name>
        <name evidence="12" type="synonym">ubash3bb</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT407062">
        <property type="protein sequence ID" value="AHH37844.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JT417013">
        <property type="protein sequence ID" value="AHH42228.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017347221.1">
        <property type="nucleotide sequence ID" value="XM_017491732.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5URI3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018656"/>
    <dbReference type="GeneID" id="108278382"/>
    <dbReference type="KEGG" id="ipu:108278382"/>
    <dbReference type="CTD" id="569827"/>
    <dbReference type="GeneTree" id="ENSGT00940000156097"/>
    <dbReference type="OMA" id="YNFPTDH"/>
    <dbReference type="OrthoDB" id="414418at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14301">
        <property type="entry name" value="UBA_UBS3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000052">
        <property type="entry name" value="Ubiquitin-associated and SH3 domain-containing protein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000162">
        <property type="entry name" value="Ubiquitin-associated and SH3 domain-containing protein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000053">
        <property type="entry name" value="Ubiquitin-associated and SH3 domain-containing, A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051710">
        <property type="entry name" value="Phosphatase_SH3-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16469">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED AND SH3 DOMAIN-CONTAINING BA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16469:SF27">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED AND SH3 DOMAIN-CONTAINING BA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22562">
        <property type="entry name" value="UBA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="UBA" evidence="9">
        <location>
            <begin position="23"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="8">
        <location>
            <begin position="243"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="6">
        <location>
            <position position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AHH42228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017347221.1"/>
        </source>
    </evidence>
    <sequence length="640" mass="71988" checksum="2A8A7C12FB2C6921" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PJ96</accession>
    <name>A0A2D0PJ96_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Nuclear receptor coactivator 3</fullName>
            <ecNumber evidence="3">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ncoa3</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SRC/p160 nuclear receptor coactivator family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017306052.1">
        <property type="nucleotide sequence ID" value="XM_017450563.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000035575"/>
    <dbReference type="GeneID" id="108255004"/>
    <dbReference type="KEGG" id="ipu:108255004"/>
    <dbReference type="CTD" id="8202"/>
    <dbReference type="OMA" id="PELVNQX"/>
    <dbReference type="OrthoDB" id="10035882at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016922">
        <property type="term" value="F:nuclear receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061733">
        <property type="term" value="F:protein-lysine-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000008">
        <property type="entry name" value="Nuclear receptor coactivator"/>
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        <property type="entry name" value="bHLH_dom"/>
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        <property type="entry name" value="HLH_DNA-bd_sf"/>
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        <property type="entry name" value="NCO_DUF1518"/>
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        <property type="entry name" value="NCOA2/3_DUF4927"/>
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        <property type="entry name" value="Nuc_rcpt_coact"/>
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        <property type="entry name" value="Nuc_rcpt_coact_Ncoa_int_sf"/>
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        <property type="entry name" value="SRC/p160_LXXLL"/>
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    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="22"/>
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        </location>
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        <location>
            <begin position="114"/>
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        <location>
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    </feature>
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        </location>
    </feature>
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            <begin position="521"/>
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            <begin position="1196"/>
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        <source>
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        <source>
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    <sequence length="1498" mass="159820" checksum="F7CF636C0E4DF1FC" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PKN2</accession>
    <name>A0A2D0PKN2_ICTPU</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein LOC108255604</fullName>
        </submittedName>
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    <gene>
        <name evidence="2" type="primary">LOC108255604</name>
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    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
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    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SDA103</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053530411.1">
        <property type="nucleotide sequence ID" value="XM_053674436.1"/>
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    <dbReference type="GeneID" id="108255604"/>
    <dbReference type="KEGG" id="ipu:108255604"/>
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        <property type="component" value="Chromosome 22"/>
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        <property type="entry name" value="Polyubiquitin 5"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ubiquitin"/>
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        <property type="entry name" value="Ubiquitin-like"/>
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        <property type="entry name" value="UBIQUITIN_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_053530411.1"/>
        </source>
    </evidence>
    <sequence length="158" mass="17896" checksum="D320344F6BFE2301" modified="2017-12-20" version="1">MMELIIKLLSGETQSVYVNPNDTVAELKQKLASLFKARPSQLKLSITNGQILQLDHDQKTVRDYGLSSGSTVMLLICTTPAPFQVFVKNEKGQTKTYDVTDDETVDELMRKIYQKERTPVDQQRLIYSGRQLESGRKLQDYNIVSGSTIHMTLRLRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PNY0</accession>
    <name>A0A2D0PNY0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Phosphatidylinositol 4,5-bisphosphate 5-phosphatase A</fullName>
            <ecNumber evidence="4">3.1.3.36</ecNumber>
            <ecNumber evidence="3">3.1.3.56</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Inositol polyphosphate 5-phosphatase J</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Phosphatidylinositol 1,3,4,5-tetrakisphosphate 5-phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Phosphatidylinositol 1,4,5-trisphosphate 5-phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">inpp5jb</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Inositol 5-phosphatase, which converts inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate. Also converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate in vitro. May be involved in modulation of the function of inositol and phosphatidylinositol polyphosphate-binding proteins that are present at membranes ruffles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>1D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O = 1D-myo-inositol 1,3,4-trisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11392"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57895"/>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
            <dbReference type="EC" id="3.1.3.56"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:11393"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1D-myo-inositol 1,4,5-trisphosphate + H2O = 1D-myo-inositol 1,4-bisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19797"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58282"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.3.56"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19798"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:22764"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="EC" id="3.1.3.36"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:22765"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type II family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.36" evidence="4"/>
    <dbReference type="EC" id="3.1.3.56" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017307707.1">
        <property type="nucleotide sequence ID" value="XM_017452218.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PNY0"/>
    <dbReference type="GeneID" id="108255881"/>
    <dbReference type="KEGG" id="ipu:108255881"/>
    <dbReference type="CTD" id="100317089"/>
    <dbReference type="OrthoDB" id="62798at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004445">
        <property type="term" value="F:inositol-polyphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034485">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09094">
        <property type="entry name" value="INPP5c_INPP5J-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2840:FF:000003">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 5-phosphatase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000013">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 5-phosphatase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041611">
        <property type="entry name" value="SKICH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF127">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE 5-PHOSPHATASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17751">
        <property type="entry name" value="SKICH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0729">SH3-binding</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="20">
        <location>
            <begin position="188"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="31"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="615"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="615"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="666"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00059259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00067189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00080358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017307707.1"/>
        </source>
    </evidence>
    <sequence length="700" mass="77925" checksum="59F3C6B49BBE6BA1" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PT49</accession>
    <name>A0A2D0PT49_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Deleted in lung and esophageal cancer protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">dlec1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017309199.2">
        <property type="nucleotide sequence ID" value="XM_017453710.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000017310"/>
    <dbReference type="GeneID" id="108256643"/>
    <dbReference type="KEGG" id="ipu:108256643"/>
    <dbReference type="CTD" id="9940"/>
    <dbReference type="OrthoDB" id="2115465at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033304">
        <property type="entry name" value="DLEC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053879">
        <property type="entry name" value="HYDIN_VesB_CFA65-like_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059041">
        <property type="entry name" value="Ig_DLEC1_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46348">
        <property type="entry name" value="DELETED IN LUNG AND ESOPHAGEAL CANCER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46348:SF1">
        <property type="entry name" value="DELETED IN LUNG AND ESOPHAGEAL CANCER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22544">
        <property type="entry name" value="HYDIN_VesB_CFA65-like_Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23277">
        <property type="entry name" value="Ig_Dlec1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23316">
        <property type="entry name" value="Ig_DLEC1_6th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Deleted in lung and esophageal cancer protein 1 Ig-like" evidence="8">
        <location>
            <begin position="308"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="HYDIN/VesB/CFA65-like Ig-like" evidence="7">
        <location>
            <begin position="1575"/>
            <end position="1670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="218"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1272"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1274"/>
            <end position="1287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017309199.2"/>
        </source>
    </evidence>
    <sequence length="1697" mass="187853" checksum="A2466D2E29D8D41D" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PTT4</accession>
    <name>A0A2D0PTT4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Sodium/potassium-transporting ATPase subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC108256310</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017308505.2">
        <property type="nucleotide sequence ID" value="XM_017453016.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PTT4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000010489"/>
    <dbReference type="GeneID" id="108256310"/>
    <dbReference type="KEGG" id="ipu:108256310"/>
    <dbReference type="OrthoDB" id="158672at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005391">
        <property type="term" value="F:P-type sodium:potassium-exchanging transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02608">
        <property type="entry name" value="P-type_ATPase_Na-K_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000079">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000003">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000001">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000004">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000095">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050510">
        <property type="entry name" value="Cation_transp_ATPase_P-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005775">
        <property type="entry name" value="P-type_ATPase_IIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01106">
        <property type="entry name" value="ATPase-IIC_X-K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294:SF10">
        <property type="entry name" value="POTASSIUM-TRANSPORTING ATPASE ALPHA CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294">
        <property type="entry name" value="SODIUM/POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00121">
        <property type="entry name" value="NAKATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="15 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12 17" id="KW-0630">Potassium</keyword>
    <keyword evidence="4 17" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 17" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="295"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="325"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="794"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="959"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="990"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="20">
        <location>
            <begin position="46"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="22"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="218"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017308505.2"/>
        </source>
    </evidence>
    <sequence length="1028" mass="114890" checksum="016948C7B6A8B6C3" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PV00</accession>
    <name>A0A2D0PV00_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Prolactin releasing hormone 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">prlh2</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017308935.1">
        <property type="nucleotide sequence ID" value="XM_017453446.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PV00"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000004865"/>
    <dbReference type="GeneID" id="108256508"/>
    <dbReference type="KEGG" id="ipu:108256508"/>
    <dbReference type="CTD" id="110437754"/>
    <dbReference type="OMA" id="GMQPVLR"/>
    <dbReference type="OrthoDB" id="8587277at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026194">
        <property type="entry name" value="PrRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17206">
        <property type="entry name" value="PROLACTIN-RELEASING PEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17206:SF0">
        <property type="entry name" value="PRRP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15172">
        <property type="entry name" value="Prolactin_RP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012632696" evidence="1">
        <location>
            <begin position="45"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017308935.1"/>
        </source>
    </evidence>
    <sequence length="129" mass="14625" checksum="08871B0362A385BC" modified="2017-12-20" version="1">MLSPGAPMSRSTRTQQHQQRSFRLRWFSVLIATLLILSTSTTCAQCTTVEQDLHIVHNVDNRSPEIDPFWYVGRGVRPIGRFGKRESDAPLQHALLLLLLSALRNTESARSDASSPNTDYYPEVHKSYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PWK3</accession>
    <name>A0A2D0PWK3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Melanocortin receptor 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">mc5ra</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. This receptor is a possible mediator of the immunomodulation properties of melanocortins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017310424.1">
        <property type="nucleotide sequence ID" value="XM_017454935.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PWK3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000015382"/>
    <dbReference type="GeneID" id="108257298"/>
    <dbReference type="KEGG" id="ipu:108257298"/>
    <dbReference type="CTD" id="286834"/>
    <dbReference type="OrthoDB" id="5970330at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004977">
        <property type="term" value="F:melanocortin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000077">
        <property type="entry name" value="Melanocortin receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001908">
        <property type="entry name" value="MC3-5R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000621">
        <property type="entry name" value="Melancort_rcpt_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001671">
        <property type="entry name" value="Melcrt_ACTH_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22750">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00534">
        <property type="entry name" value="MCRFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00535">
        <property type="entry name" value="MELNOCORTINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01063">
        <property type="entry name" value="MELNOCORTN5R"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="245"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="62"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00025181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017310424.1"/>
        </source>
    </evidence>
    <sequence length="332" mass="37724" checksum="AA431CDA1FA5FB93" modified="2017-12-20" version="1">MNFSKTTTVSPSVWSFMVNSSQEVYSLNGTDAPPSRTKSKVCEQLNITPEVFLILGIISLLENILVICAIVKNKNLHSPMYFFVCSLAVADMLVSVSNAWETIVIYLLSNQQLVAEDHIIRQIDNVFDSMICISVVASMCSLLAIAVDRYVTTFYALRYHNIMTVKRASLIITGIWTFCTGCGIVFIIYSDSTLVIVCLVSMFFIMLALMASLYTHMFMLARSHVKRIAALPGYNSIHQRTNMKAAVTLTILLGIFIVCWAPFFLHLILMISCPRNLYCMCFMSHFNMYLILIMCNSVIDPLIYAFRSQEMRKTLKEIICCYSLRNVLRWSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PWT7</accession>
    <name>A0A2D0PWT7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13 14">P2Y purinoceptor 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13 14" type="primary">p2ry11</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13 14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017310487.1">
        <property type="nucleotide sequence ID" value="XM_017454998.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017310488.1">
        <property type="nucleotide sequence ID" value="XM_017454999.3"/>
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    <sequence length="302" mass="34643" checksum="05FAC5754C347600" modified="2017-12-20" version="1">MAAVINSSNSSYSSNCNSTFSKVLLPPIYSVEMCVALVGNILALWLLVTKERKNWHTGVVFSCNLVISDILYALTLPLLIDYYSRERKWIFGHTVCKIERFLFTCNLYVSIYFIMCISVNRYLAIVHPFFARNHVQPKHAKIISFLVWILVASISSPILYYSGTKGKRCSLFSDSNSEPKKFMYRVFMAVVGCLVPFLLTFASYFGVIWVVFKNENITSMEKKKVALMVGLVCVLYSMSFVPYHILQIWHFKLKGAKIYNYYVCNGYQVSKALACVNMCLHPILYMAVFDSIRTMCCRRSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PXJ2</accession>
    <name>A0A2D0PXJ2_ICTPU</name>
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        <submittedName>
            <fullName evidence="10">MICAL-like protein 2a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">micall2a</name>
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    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
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    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
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    </reference>
    <dbReference type="RefSeq" id="XP_017310151.3">
        <property type="nucleotide sequence ID" value="XM_017454662.3"/>
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        <property type="component" value="Chromosome 24"/>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="bMERB_dom"/>
    </dbReference>
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        <property type="entry name" value="CH_dom"/>
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        <property type="entry name" value="CH_dom_sf"/>
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    </dbReference>
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        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167">
        <property type="entry name" value="CALPONIN HOMOLOGY DOMAIN-CONTAINING PROTEIN DDB_G0272472-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MICAL-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="7">
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            <end position="294"/>
        </location>
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            <begin position="617"/>
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        </location>
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            <begin position="115"/>
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            <begin position="628"/>
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            <begin position="743"/>
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            <begin position="122"/>
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    </feature>
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    </evidence>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51848"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017310151.3"/>
        </source>
    </evidence>
    <sequence length="803" mass="88967" checksum="956DD75B54C853EA" modified="2023-06-28" version="3"> </sequence>
</entry>
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    <accession>A0A2D0PYG3</accession>
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        <submittedName>
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        <name evidence="4 5" type="primary">cracd</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
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            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4 5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017311538.1">
        <property type="nucleotide sequence ID" value="XM_017456049.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017311539.1">
        <property type="nucleotide sequence ID" value="XM_017456050.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PYG3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000015858"/>
    <dbReference type="GeneID" id="108257906"/>
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        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030277">
        <property type="term" value="P:maintenance of gastrointestinal epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000813">
        <property type="term" value="P:negative regulation of barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Actin_dynamics_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028030">
        <property type="entry name" value="DUF4592"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47574">
        <property type="entry name" value="CANCER-RELATED REGULATOR OF ACTIN DYNAMICS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47574:SF3">
        <property type="entry name" value="CAPPING PROTEIN-INHIBITING REGULATOR OF ACTIN DYNAMICS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15262">
        <property type="entry name" value="DUF4592"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4592" evidence="2">
        <location>
            <begin position="19"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="511"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="561"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="297"/>
        </location>
    </feature>
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        <location>
            <begin position="529"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="565"/>
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        </location>
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            <begin position="589"/>
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        </location>
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        </location>
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        </location>
    </feature>
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            <begin position="706"/>
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        <location>
            <begin position="1011"/>
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        </location>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="Pfam" id="PF15262"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017311538.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017311539.1"/>
        </source>
    </evidence>
    <sequence length="1095" mass="125578" checksum="7E473033B7C6F75A" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PZF2</accession>
    <name>A0A2D0PZF2_ICTPU</name>
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        <submittedName>
            <fullName evidence="3">Sterile alpha motif domain-containing protein 12 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">samd12</name>
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        <name type="scientific">Ictalurus punctatus</name>
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        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017310495.1">
        <property type="nucleotide sequence ID" value="XM_017455006.3"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="434324at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Transcription Factor, Ets-1"/>
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    </dbReference>
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        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN CONTAINING PROTEIN 10"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="100"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017310495.1"/>
        </source>
    </evidence>
    <sequence length="196" mass="22199" checksum="910F514C3648E303" modified="2017-12-20" version="1">MMLGQSPHLVGGILWIQNPMRAVHCNLSQNGIDHQVCMADASSHVEEESVVRGAGLIDDSSGFQHLSPGFQCCTPPQRSVSESELSKSGTVKLSKPVALWTQQDVCKWLKKHCPNQHQVYSDAFKQHDITGRALMRLTDRKLERMGIMQESQRQYILQQVLQLRVREEVRTLQLLTQDLVPFCCYKNLQSSGKAFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q058</accession>
    <name>A0A2D0Q058_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 15">Ribonuclease H1</fullName>
            <shortName evidence="15">RNase H1</shortName>
            <ecNumber evidence="6 15">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19 20" type="primary">rnaseh1</name>
    </gene>
    <organism evidence="18 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19 20" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19 20">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Plays a role in RNA polymerase II (RNAp II) transcription termination by degrading R-loop RNA-DNA hybrid formation at G-rich pause sites located downstream of the poly(A) site and behind the elongating RNAp II.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 21 22">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the RNase H family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="6 15"/>
    <dbReference type="RefSeq" id="XP_017312127.1">
        <property type="nucleotide sequence ID" value="XM_017456638.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017312128.1">
        <property type="nucleotide sequence ID" value="XM_017456639.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q058"/>
    <dbReference type="GeneID" id="108258173"/>
    <dbReference type="CTD" id="246243"/>
    <dbReference type="OMA" id="ELWYGLY"/>
    <dbReference type="OrthoDB" id="407198at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09280">
        <property type="entry name" value="RNase_HI_eukaryote_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000049">
        <property type="entry name" value="Ribonuclease H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.970.10:FF:000001">
        <property type="entry name" value="Ribonuclease H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.970.10">
        <property type="entry name" value="Ribonuclease H1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_bL9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050092">
        <property type="entry name" value="RNase_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017067">
        <property type="entry name" value="RNase_H1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011320">
        <property type="entry name" value="RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037056">
        <property type="entry name" value="RNase_H1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
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        <property type="entry name" value="RIBONUCLEASE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10642:SF26">
        <property type="entry name" value="RIBONUCLEASE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01693">
        <property type="entry name" value="Cauli_VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036852">
        <property type="entry name" value="Ribonuclease_H1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="L9 N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 15" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0540">Nuclease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="17">
        <location>
            <begin position="108"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="52"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00059147"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00068459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR036852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017312127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017312128.1"/>
        </source>
    </evidence>
    <sequence length="258" mass="28630" checksum="DB95609DC7A3A638" modified="2017-12-20" version="1">MGKKGSFFYAVKKGHRPGVYQTWEECKFQVDRFPDARYKKFGREDEAWAFVRNESTSTENSEATPRTPSVPVGSKRSFEGDEVDGGSVKRAKLGPSAANSSTSALTYMGDAVVVYTDGCCTQNGRHGARAGIGVYWGPSHPLNVAERLPGRQTNQRAELQAACKALEQAIHMEIKKLVVYTDSKFTINGITSWVKKWKTNGWRLQTGGKVVNKEDFQKLDELNEGLDVKWMHIPGHAGYLGNEEADRLSREGAAKPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q910</accession>
    <name>A0A2D0Q910_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Lysophosphatidylcholine acyltransferase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">lpcat2</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="16">Phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017314782.1">
        <property type="nucleotide sequence ID" value="XM_017459293.3"/>
    </dbReference>
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    <dbReference type="STRING" id="7998.ENSIPUP00000025125"/>
    <dbReference type="GeneID" id="108259642"/>
    <dbReference type="KEGG" id="ipu:108259642"/>
    <dbReference type="CTD" id="54947"/>
    <dbReference type="OMA" id="FLYHKSE"/>
    <dbReference type="OrthoDB" id="272512at2759"/>
    <dbReference type="UniPathway" id="UPA00085"/>
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        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047184">
        <property type="term" value="F:1-acylglycerophosphocholine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042171">
        <property type="term" value="F:lysophosphatidic acid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd07991">
        <property type="entry name" value="LPLAT_LPCAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calmodulin-2 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045252">
        <property type="entry name" value="LPCAT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063:SF21">
        <property type="entry name" value="LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063">
        <property type="entry name" value="PHOSPHOLIPID ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 20" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="18">
        <location>
            <begin position="372"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="18">
        <location>
            <begin position="409"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="18">
        <location>
            <begin position="448"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017314782.1"/>
        </source>
    </evidence>
    <sequence length="525" mass="59660" checksum="CCD1D4EDAA40F555" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QCP9</accession>
    <name>A0A2D0QCP9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Transcription factor HES-7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">her1</name>
        <name evidence="12" type="synonym">LOC128631200</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11 12">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017316069.1">
        <property type="nucleotide sequence ID" value="XM_017460580.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053535252.1">
        <property type="nucleotide sequence ID" value="XM_053679277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QCP9"/>
    <dbReference type="GeneID" id="108260347"/>
    <dbReference type="KEGG" id="ipu:108260347"/>
    <dbReference type="KEGG" id="ipu:128631200"/>
    <dbReference type="CTD" id="30287"/>
    <dbReference type="GeneTree" id="ENSGT00730000111282"/>
    <dbReference type="OMA" id="NTFVLHH"/>
    <dbReference type="OrthoDB" id="690068at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11462">
        <property type="entry name" value="bHLH-O_HES7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000063">
        <property type="entry name" value="transcription factor HES-7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032644">
        <property type="entry name" value="HES-7_bHLH-O"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="9">
        <location>
            <begin position="12"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017316069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053535252.1"/>
        </source>
    </evidence>
    <sequence length="302" mass="33602" checksum="A383C36C8E4F504B" modified="2017-12-20" version="1">MGTPKMSNRRASRRVLKPVIEKKRRDRINQRLDELRTLLLDHTLDSRLQNPKLEKAEILELTVEYIRKKATNTKENAAEIHRDPGECVPVALAQTAGRPRVPVTGLHDGPHVHAHAPTSPIYTAGFQECISRLTSFIECVDLSQRENFIQCLRHHLQSHTGVLSQVRGHGQTHTWVTGDVRAGAEPFSFANGLYPNSFVLHPPYPSPPYSLSPPPSPCYSSSSPTYLSVPRHFHYPPSVSPLSDSSSTSSSSFTTSTKAVSASLAQATQAPPRPLTPHCVSAQQTLRRELFPSHTHAIWRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QNG9</accession>
    <name>A0A2D0QNG9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Interferon-induced protein with tetratricopeptide repeats 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ifit8</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the IFIT family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017319942.1">
        <property type="nucleotide sequence ID" value="XM_017464453.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QNG9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006542"/>
    <dbReference type="GeneID" id="108263543"/>
    <dbReference type="KEGG" id="ipu:108263543"/>
    <dbReference type="CTD" id="641567"/>
    <dbReference type="OMA" id="RIDCPEM"/>
    <dbReference type="OrthoDB" id="10043504at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000032">
        <property type="entry name" value="Interferon-induced protein with tetratricopeptide repeats 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10271">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN WITH TETRATRICOPEPTIDE REPEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10271:SF25">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN WITH TETRATRICOPEPTIDE REPEATS 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017319942.1"/>
        </source>
    </evidence>
    <sequence length="432" mass="51034" checksum="ACC44C36CDFE2791" modified="2017-12-20" version="1">MFLEELKKLECHFTWDLQKEDADLNYLEIKFTESLSVQSDHKGNFRQRELNFCAYIHHLQGFSDKARECLEKAKEVQNDDSYNIVTYGNLAWLHHHMADDNMARVYLEKLAQISGAFPSPAEELHREVLSEKAWSFLRFSKKHYIKAKEIFQEVLEKEPEDKEWNTGYAFALFHLEGLEIREDKRIPFEESPAVSQLKRALKLDPDNAMIHVFMGLKCNKNERNSEAWGHMKQALDMAPYDLSVVLSVAKFMKKEQCYDMALGVLKKMLEKAPDSSRLHHEIGNNYRWKAMQLRDIHNPKLLSHCIYHLEEGARLNPDFIYPQIELALRYAEVGNYTKAKQKFQELFARPNLQLADLQAWHRIYGDFNMYRLGSEATAVKHYKEGMALQKVSTEWRICKNRLHKVLHNTRNDVYQIREFMKSLRENNERTEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QTI8</accession>
    <name>A0A2D0QTI8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein si:ch211-14c7.2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">si:ch211-14c7.2</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017320810.1">
        <property type="nucleotide sequence ID" value="XM_017465321.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QTI8"/>
    <dbReference type="GeneID" id="108264030"/>
    <dbReference type="KEGG" id="ipu:108264030"/>
    <dbReference type="OrthoDB" id="8965081at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="564"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="765"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="564"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017320810.1"/>
        </source>
    </evidence>
    <sequence length="807" mass="89393" checksum="015FFB3D78ACDE8D" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QVE9</accession>
    <name>A0A2D0QVE9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Serine/threonine-protein kinase NIM1</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">NIM1 serine/threonine-protein kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21 22" type="primary">nim1ka</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21 22" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21 22">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017322397.1">
        <property type="nucleotide sequence ID" value="XM_017466908.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053535981.1">
        <property type="nucleotide sequence ID" value="XM_053680006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QVE9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000028409"/>
    <dbReference type="GeneID" id="108264912"/>
    <dbReference type="KEGG" id="ipu:108264912"/>
    <dbReference type="CTD" id="100536336"/>
    <dbReference type="GeneTree" id="ENSGT00940000165543"/>
    <dbReference type="OMA" id="IMSNEWM"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14075">
        <property type="entry name" value="STKc_NIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000346">
        <property type="entry name" value="Serine/threonine-protein kinase NIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049571">
        <property type="entry name" value="NIM1K_STKc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF35">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NIM1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 21 22" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="73"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="17"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00080118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017322397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_053535981.1"/>
        </source>
    </evidence>
    <sequence length="436" mass="49406" checksum="7CBA2D5756C7BB16" modified="2017-12-20" version="1">MMTAVYPADVGVPSAACPQRHSRCTGQDSSDVLTDDEGVPERRRTPLEQLNLDMCQDDRVVREITIGRRIGFYKICGEIGCGNFSHVKLGVHALTKDKVAIKILDKTKLDQKTQKLLSREISSMEKLQHPNIIRLYEVVETLTRLHLVMEYAGGGELYTKITMEGKLSDIDSKIVFSQILSAVKHMHDNNIIHRDLKAENVFFACSTCVKVGDFGFSTVSRRDETLSTLCGSPPYAAPELFRDERYVGVYVDIWALGVMLFFMVSGTMPFRADTVAKLKRCILDGAFSVPAWVPEPCQRLIHGILQPQPSERCSLERMMNSEWLENVDFPKATEPFKLDPLYLAEEEDLEEDECEVKNSLQALGITTEHIRNNQGKDCRSSVTGVYRILLHRAHRRRAFESMPLVTHNVSEPNEAKKERLKTYKSLRHTSKLCAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QVT1</accession>
    <name>A0A2D0QVT1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Serine/threonine-protein kinase SBK1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">si:dkey-8e10.3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017322532.1">
        <property type="nucleotide sequence ID" value="XM_017467043.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QVT1"/>
    <dbReference type="GeneID" id="108264997"/>
    <dbReference type="KEGG" id="ipu:108264997"/>
    <dbReference type="OMA" id="HRQKGTT"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13987">
        <property type="entry name" value="STKc_SBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000999">
        <property type="entry name" value="Si:dkey-8e10.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359:SF34">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="55"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="333"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="344"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017322532.1"/>
        </source>
    </evidence>
    <sequence length="357" mass="40045" checksum="FE507C4BE38C0D60" modified="2017-12-20" version="1">MKEKISSTCNDQTHKTVQGRAMIELGPVEDGSSHIEELMELTAQCLSRLEIKEHFNVIKEIGRGKYGRVLLVTHRCRGTPMALKVLPKASTQLKGFLREYCISLRLSSHPCIVGLFGIAFQSNEHYGFAQELVVGRDLFAIIKPRVGISESVVKRCAVQIASALEFIHRLGLVHRDIKPENILLLDTQCRHVKLADFGLTQKRGTLICYISSTLPYMAPELCAMVAEDGQEKPQAPPLSIQPSLDTWAFGVLLFCILTGFFPWEHCTSTDSFYEEFAEWRQDTETTEIPTQWKRFTPACLEMFGKLLALEPNDRSTADVVKGYVGKEWVKPKRPNESEGVNAMATLSSPCRSSPVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QVW9</accession>
    <name>A0A2D0QVW9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peroxisomal 2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing]</fullName>
            <ecNumber evidence="9">1.3.1.124</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">2,4-dienoyl-CoA reductase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16 17" type="primary">decr2</name>
    </gene>
    <organism evidence="15 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16 17" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16 17">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(2E,4Z,7Z,10Z,13Z,16Z,19Z)-docosaheptaenoyl-CoA + NADPH + H(+) = (3E,7Z,10Z,13Z,16Z,19Z)-docosahexaenoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:44920"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:77559"/>
            <dbReference type="ChEBI" id="CHEBI:84791"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a (2E,4E)-dienoyl-CoA + NADPH + H(+) = a 4,5-saturated-(3E)-enoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:45912"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85101"/>
            <dbReference type="ChEBI" id="CHEBI:85493"/>
            <dbReference type="EC" id="1.3.1.124"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a (2E,4Z)-dienoyl-CoA + NADPH + H(+) = a 4,5-saturated-(3E)-enoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:61892"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85099"/>
            <dbReference type="ChEBI" id="CHEBI:85493"/>
            <dbReference type="EC" id="1.3.1.124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer, dimer and oligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2,4-dienoyl-CoA reductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.124" evidence="9"/>
    <dbReference type="RefSeq" id="XP_017321877.1">
        <property type="nucleotide sequence ID" value="XM_017466388.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017321895.1">
        <property type="nucleotide sequence ID" value="XM_017466406.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QVW9"/>
    <dbReference type="GeneID" id="108264653"/>
    <dbReference type="KEGG" id="ipu:108264653"/>
    <dbReference type="CTD" id="26063"/>
    <dbReference type="OrthoDB" id="1393670at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008670">
        <property type="term" value="F:2,4-dienoyl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05369">
        <property type="entry name" value="TER_DECR_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000477">
        <property type="entry name" value="Peroxisomal 2,4-dienoyl-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045017">
        <property type="entry name" value="DECR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43296">
        <property type="entry name" value="PEROXISOMAL 2,4-DIENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43296:SF2">
        <property type="entry name" value="PEROXISOMAL 2,4-DIENOYL-COA REDUCTASE [(3E)-ENOYL-COA-PRODUCING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017321877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017321895.1"/>
        </source>
    </evidence>
    <sequence length="294" mass="31643" checksum="714C96C9CA566C27" modified="2017-12-20" version="1">MAEAPEDVHTDECLSTYTHIYSQDLLKDQVAFITGGGSGIGFRITEVLMRHGCDTVIASRNLEKLTEAAKKLTSATGRHCLPLQMDVRQPDTISTAVDEMLKEFGRVDILINNAAGNFLCPAAALSFNAFKTVLEIDTMGTFNTSKVVYEKWFKDHGGSIVNISATLGYKGQALQVHAGSAKAANDAMTRHLAVEWGPSGVRVNTVAPGPISGTEGYRRLGGSHAESAGIFRTIPLQRAGNKTEMAHTVLFLASRAASYVTGATVVADGGAWLTSANDVERLLDLWSQEKRKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QW52</accession>
    <name>A0A2D0QW52_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Potassium voltage-gated channel subfamily C member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">kcnc4</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Presynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017322534.1">
        <property type="nucleotide sequence ID" value="XM_017467045.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QW52"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006262"/>
    <dbReference type="GeneID" id="108264999"/>
    <dbReference type="KEGG" id="ipu:108264999"/>
    <dbReference type="CTD" id="3749"/>
    <dbReference type="OrthoDB" id="10025005at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043679">
        <property type="term" value="C:axon terminus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032590">
        <property type="term" value="C:dendrite membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032809">
        <property type="term" value="C:neuronal cell body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <source>
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    <sequence length="599" mass="67574" checksum="6940DDF21E74D3E1" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QWW9</accession>
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        <submittedName>
            <fullName evidence="15">Melanocortin receptor 3</fullName>
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        <name evidence="15" type="primary">mc3r</name>
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    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
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    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="14">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <tissue evidence="15">Blood</tissue>
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    </reference>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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        <property type="entry name" value="MC3-5R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001671">
        <property type="entry name" value="Melcrt_ACTH_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22750">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GPCRRHODOPSN"/>
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        <property type="entry name" value="MCRFAMILY"/>
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        <property type="entry name" value="MELNOCORTINR"/>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    </dbReference>
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    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
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    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 10 12" id="KW-0812">Transmembrane</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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    <feature type="transmembrane region" description="Helical" evidence="12">
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            <begin position="173"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="257"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="300"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="67"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="341"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="342"/>
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        <source>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="ARBA" id="ARBA00023170"/>
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            <dbReference type="ARBA" id="ARBA00023224"/>
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        <source>
            <dbReference type="RuleBase" id="RU000688"/>
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        <source>
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            <dbReference type="PROSITE" id="PS50262"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017322804.3"/>
        </source>
    </evidence>
    <sequence length="367" mass="41044" checksum="492F87068C51C772" modified="2023-06-28" version="3">MNGSYHHMLLRDSSLTNLTEETEYENGSTSLWLSNGSVAPPPSGALCQQVQIQAEVFLALGIVSLLENILVISAVAKNKNLHSPMYFFLCSLAAADMLVSVSNSLETIVIAILRNNVLKLSDFFVRLMDNIFDSMICISLVASICNLLAIAIDRYVTIFYALRYHSIVTARRALSAIGIIWLTCIICGIVFIIYSESKMVIICLITMFFAMLALMATLYVHMFLLARLHVQRIAALPAAAVAAGDPAPRQRSCLKGAVTISILLGVFVCCWAPFFLHLILLVACPRHPLCLCYMSHFTTYLVLIMCNSVIDPIIYAFRSLEMRNTFREILCCFGTGCPSPGCEREREMDPERVRERRQEREKESETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QX91</accession>
    <name>A0A2D0QX91_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Carbonic anhydrase 15 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">car15</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017322361.1">
        <property type="nucleotide sequence ID" value="XM_017466872.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QX91"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012014"/>
    <dbReference type="GeneID" id="108264895"/>
    <dbReference type="KEGG" id="ipu:108264895"/>
    <dbReference type="CTD" id="80733"/>
    <dbReference type="OrthoDB" id="429145at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF134">
        <property type="entry name" value="CARBONIC ANHYDRASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013379493" evidence="2">
        <location>
            <begin position="20"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="3">
        <location>
            <begin position="30"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017322361.1"/>
        </source>
    </evidence>
    <sequence length="324" mass="36046" checksum="CA2748C584609F04" modified="2017-12-20" version="1">MQSWMSALISVLMLSTVSALSLKGFYVPPLNYCYTEDSCSPYNWVKMFPTCIAKESGLHSPINLQRVVIKNNSIDPLELQGFDVPQKSWTVVNVRDTVVVKFQAGMTVKGGSIKHNYRIVEMRFHWGSNTTNGSEHKLDARRFPMEMQIVGVAPGFADVEAASVVQSGLLIMGVFIDIASEENQAFKAIAHAVLNVPYPGDSVSASPPALSHLLPEDGTFYQYHGGQTTPPCRQTVTWIVFEKPIFISRKQHLPFITQLYYSDENDTVKKLLVGNYRFIQPSLNRQIFVSSAVKIRSASGAALSQRTLFIPLLILASELSHTML</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QXA6</accession>
    <name>A0A2D0QXA6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Hepatocyte nuclear factor 1-alpha isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">hnf1a</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="9">Binds DNA as a dimer. Can form homodimer or heterodimer with HNF1-alpha.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HNF1 homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017322376.1">
        <property type="nucleotide sequence ID" value="XM_017466887.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QXA6"/>
    <dbReference type="GeneID" id="108264904"/>
    <dbReference type="KEGG" id="ipu:108264904"/>
    <dbReference type="CTD" id="6927"/>
    <dbReference type="OrthoDB" id="10069265at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034672">
        <property type="term" value="P:anterior/posterior pattern specification involved in pronephros development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030073">
        <property type="term" value="P:insulin secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001889">
        <property type="term" value="P:liver development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031016">
        <property type="term" value="P:pancreas development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000043">
        <property type="entry name" value="Hepatocyte nuclear factor 1-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000009">
        <property type="entry name" value="Hepatocyte nuclear factor 1-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039066">
        <property type="entry name" value="HNF-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006899">
        <property type="entry name" value="HNF-1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044869">
        <property type="entry name" value="HNF-1_POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023219">
        <property type="entry name" value="HNF1_dimer_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006898">
        <property type="entry name" value="HNF1a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006897">
        <property type="entry name" value="HNF1b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044866">
        <property type="entry name" value="HNF_P1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11568">
        <property type="entry name" value="HEPATOCYTE NUCLEAR FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11568:SF4">
        <property type="entry name" value="HEPATOCYTE NUCLEAR FACTOR 1-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04814">
        <property type="entry name" value="HNF-1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04813">
        <property type="entry name" value="HNF-1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04812">
        <property type="entry name" value="HNF-1B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100957">
        <property type="entry name" value="Dimerization cofactor of HNF-1 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51937">
        <property type="entry name" value="HNF_P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51936">
        <property type="entry name" value="POU_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="4 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HNF-p1" evidence="14">
        <location>
            <begin position="12"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="POU-specific atypical" evidence="13">
        <location>
            <begin position="88"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="200"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="202"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="43"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="284"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="509"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="532"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017322376.1"/>
        </source>
    </evidence>
    <sequence length="580" mass="63112" checksum="B287AA7C0A0435F6" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QZU0</accession>
    <name>A0A2D0QZU0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transketolase</fullName>
            <ecNumber evidence="9">2.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC108265455</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = aldehydo-D-ribose 5-phosphate + D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:10508"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the transketolase family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.1" evidence="9"/>
    <dbReference type="RefSeq" id="XP_017323281.1">
        <property type="nucleotide sequence ID" value="XM_017467792.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QZU0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000036274"/>
    <dbReference type="GeneID" id="108265455"/>
    <dbReference type="KEGG" id="ipu:108265455"/>
    <dbReference type="OMA" id="VYCLCGD"/>
    <dbReference type="OrthoDB" id="10267175at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004802">
        <property type="term" value="F:transketolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07033">
        <property type="entry name" value="TPP_PYR_DXS_TK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02012">
        <property type="entry name" value="TPP_TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000028">
        <property type="entry name" value="Transketolase isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000033">
        <property type="entry name" value="Transketolase isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000008">
        <property type="entry name" value="transketolase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051424">
        <property type="entry name" value="Transketolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020826">
        <property type="entry name" value="Transketolase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049557">
        <property type="entry name" value="Transketolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004559">
        <property type="entry name" value="PRK05899.2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43195">
        <property type="entry name" value="TRANSKETOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43195:SF3">
        <property type="entry name" value="TRANSKETOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00456">
        <property type="entry name" value="Transketolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00801">
        <property type="entry name" value="TRANSKETOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00802">
        <property type="entry name" value="TRANSKETOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0007">Acetylation</keyword>
    <keyword evidence="15" id="KW-0106">Calcium</keyword>
    <keyword evidence="11" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <keyword evidence="17" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="22">
        <location>
            <begin position="315"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00002931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00007131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00013152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00016662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_017323281.1"/>
        </source>
    </evidence>
    <sequence length="629" mass="69146" checksum="2D145F8538DFA20B" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R2Q4</accession>
    <name>A0A2D0R2Q4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">usp9</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12 13">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017324886.1">
        <property type="nucleotide sequence ID" value="XM_017469397.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017324887.1">
        <property type="nucleotide sequence ID" value="XM_017469398.3"/>
    </dbReference>
    <dbReference type="GeneID" id="108266305"/>
    <dbReference type="KEGG" id="ipu:108266305"/>
    <dbReference type="CTD" id="568683"/>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02659">
        <property type="entry name" value="peptidase_C19C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056850">
        <property type="entry name" value="ARM_UBP34_24_USP9X_Y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021905">
        <property type="entry name" value="DUF3517"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055176">
        <property type="entry name" value="UBP24/USP9X/USP9Y_UBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF925">
        <property type="entry name" value="UBIQUITINYL HYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25010">
        <property type="entry name" value="ARM_UBP24_USP9X-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12030">
        <property type="entry name" value="DUF3517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22900">
        <property type="entry name" value="UCH_UBL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="10">
        <location>
            <begin position="1572"/>
            <end position="1973"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="983"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1607"/>
            <end position="1628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="2503"/>
            <end position="2607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="989"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1616"/>
            <end position="1628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="2533"/>
            <end position="2542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="2556"/>
            <end position="2567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017324886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017324887.1"/>
        </source>
    </evidence>
    <sequence length="2607" mass="296215" checksum="5F1158F9A905C0ED" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R6H5</accession>
    <name>A0A2D0R6H5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Epidermal growth factor-like protein 6 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">egfl6</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nephronectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017326198.1">
        <property type="nucleotide sequence ID" value="XM_017470709.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R6H5"/>
    <dbReference type="GeneID" id="108266885"/>
    <dbReference type="KEGG" id="ipu:108266885"/>
    <dbReference type="CTD" id="25975"/>
    <dbReference type="OrthoDB" id="10060424at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000049">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000187">
        <property type="entry name" value="nephronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000476">
        <property type="entry name" value="nephronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052235">
        <property type="entry name" value="Nephronectin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24050:SF24">
        <property type="entry name" value="EPIDERMAL GROWTH FACTOR-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24050">
        <property type="entry name" value="PA14 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="11" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 13" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013333926" evidence="15">
        <location>
            <begin position="22"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="171"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="216"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="17">
        <location>
            <begin position="396"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="324"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
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    <sequence length="550" mass="61641" checksum="6BE8E2D3DB718CF3" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R7W9</accession>
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        <submittedName>
            <fullName evidence="3">Regulator of G-protein signaling 5b</fullName>
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        <name evidence="3" type="primary">rgs5b</name>
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        <name type="scientific">Ictalurus punctatus</name>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
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                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">SDA103</strain>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Liu S."/>
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                <person name="Bao L."/>
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                <person name="Jiang C."/>
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                <person name="Geng X."/>
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                <person name="Yuan Z."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="entry name" value="PH_domain"/>
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        <property type="entry name" value="MYOSIN PHOSPHATASE RHO-INTERACTING PROTEIN"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017328626.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053540782.1"/>
        </source>
    </evidence>
    <sequence length="2413" mass="275740" checksum="9E1407D11892C073" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RFY8</accession>
    <name>A0A2D0RFY8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Four and a half LIM domains protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">fhl1a</name>
        <name evidence="18" type="synonym">fhl1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">May have an involvement in muscle development or hypertrophy. Isoform 2 binds to RBP-J and plays a negative regulatory role in the RBP-J-mediated transcription in mammalian systems.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017328810.1">
        <property type="nucleotide sequence ID" value="XM_017473321.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RFY8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032833"/>
    <dbReference type="GeneID" id="100529032"/>
    <dbReference type="CTD" id="399646"/>
    <dbReference type="OrthoDB" id="1112565at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09344">
        <property type="entry name" value="LIM1_FHL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09424">
        <property type="entry name" value="LIM2_FHL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09429">
        <property type="entry name" value="LIM3_FHL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09348">
        <property type="entry name" value="LIM4_FHL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000013">
        <property type="entry name" value="Four and a half LIM domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000052">
        <property type="entry name" value="Four and a half LIM domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000050">
        <property type="entry name" value="Four and a half LIM domains protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000072">
        <property type="entry name" value="Four and a half LIM domains protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042997">
        <property type="entry name" value="Fhl1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47029">
        <property type="entry name" value="FOUR AND A HALF LIM DOMAINS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47029:SF2">
        <property type="entry name" value="FOUR AND A HALF LIM DOMAINS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12 15" id="KW-0440">LIM domain</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="16">
        <location>
            <begin position="54"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="16">
        <location>
            <begin position="116"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="16">
        <location>
            <begin position="178"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="16">
        <location>
            <begin position="236"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00074675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017328810.1"/>
        </source>
    </evidence>
    <sequence length="297" mass="34400" checksum="F4FFABFC05A23FD4" modified="2017-12-20" version="1">MTFYRHSGPRSYTSSIMTERFNCFYCRDDLHGKKYIQKDEKHVCVRCFDKLCANTCAECRRPIGADAKELTHKNRHWHEDCFRCAKCYKPLANESFATKDDGKIMCGKCGAREDSPRCQGCYKVIMPGSQNVEYKHKVWHEECFICFECKQPIRSQSFLPKGDEFYCSACHEKKFAKNCARCKEPITSGGINYQDKPWHSECFVCNTCKKPLAGARFTAHEDDFYCVDCYKTSVAKKCSGCQNPITGFGRGTNVVNYEDHTWHEYCFNCKKCSLSLAHKRFVLHEENIYCPDCAKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RGT2</accession>
    <name>A0A2D0RGT2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">D site albumin promoter binding protein b isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">dbpb</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family. PAR subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017329110.1">
        <property type="nucleotide sequence ID" value="XM_017473621.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RGT2"/>
    <dbReference type="SMR" id="A0A2D0RGT2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000011824"/>
    <dbReference type="GeneID" id="108268581"/>
    <dbReference type="KEGG" id="ipu:108268581"/>
    <dbReference type="CTD" id="569109"/>
    <dbReference type="OMA" id="HRLKNPC"/>
    <dbReference type="OrthoDB" id="6022300at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14695">
        <property type="entry name" value="bZIP_HLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000007">
        <property type="entry name" value="hepatic leukemia factor isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040223">
        <property type="entry name" value="PAR_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988:SF7">
        <property type="entry name" value="D SITE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988">
        <property type="entry name" value="THYROTROPH EMBRYONIC FACTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BSD" evidence="10">
        <location>
            <begin position="253"/>
            <end position="288"/>
        </location>
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    <feature type="domain" description="BZIP" evidence="9">
        <location>
            <begin position="273"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="138"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="185"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="305"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="141"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="193"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017329110.1"/>
        </source>
    </evidence>
    <sequence length="344" mass="37488" checksum="D7110B05D40A03B9" modified="2017-12-20" version="1">MMARPPSQRLLPPSGPKIHFRSLDHAGGDSLNTGPVNTGPDLKSPPQTTLKGDQSFKDCCEMKDKDRLDMDEDSLGRCSIRDRNSNGQVADGSGLGTGPGTFQSLWERTVASDPGLFQLHYMDLGEFLTENGMAMHGAQIPSQSSQCPSTSPSPCSSSSSSSASAASPPTLLGLDMQVQQSMLGSAGCLHSPPQGSLEPTPSSSCPPLTAPPMTNANDMLTTFDPDPADVALSSVPGQEAFDPRTHSFTEEDLKPQPVIKKARKMLVPDSLKDDKYWSRRLKNNEAAKRSRDARRVKENQISVRAAFLERENAVLRQEVANMRKELGRCRNILNKYESRYVEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RID7</accession>
    <name>A0A2D0RID7_ICTPU</name>
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        <submittedName>
            <fullName evidence="3">ADP-ribose glycohydrolase MACROD1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">macrod1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017330287.1">
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        <property type="component" value="Chromosome 8"/>
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        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:DNA damage response"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:peptidyl-glutamate ADP-deribosylation"/>
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    </dbReference>
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        <property type="term" value="P:purine nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Macro_OAADPr_deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
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        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001664">
        <property type="entry name" value="PRK00431.1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106:SF93">
        <property type="entry name" value="ADP-RIBOSE GLYCOHYDROLASE MACROD1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GANGLIOSIDE INDUCED DIFFERENTIATION ASSOCIATED PROTEIN 2-RELATED"/>
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        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="1">
        <location>
            <begin position="165"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017330287.1"/>
        </source>
    </evidence>
    <sequence length="356" mass="38811" checksum="F4BC0FE263464108" modified="2017-12-20" version="1">MAFQISKLVARVSVSVLSRVLEKQKLSRGVNFSLFHTALNSPPSARLGINIAARSEKFSTSSATSRWFREGKGFSVSRGVLSRKGSVVVLFGTVGVSAVVLSSSAVCAMAARSPLNLDSDKSDWKDVKKELLSMSLKERREMYRTDYISLDQVPVWKHSGTVSAKPLYKANDDLSQKISLFRGDITKLEIDAVANAANKTLLGGGGVDGAIHRGAGPLLRKECATLGGCETGEAKITGAYGLPARYIIHTVGPIAHGSVGETERRALRDCYYNCLRTATENQLRTVAFPCISTGVYGYPPEQAVDVALKTVREYLEDNPAQMDRVIFCVFLKSDEDLYKGRLPAYFPKDAFPKSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RMD1</accession>
    <name>A0A2D0RMD1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Prolactin releasing hormone 2 receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">prlh2r</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017331105.2">
        <property type="nucleotide sequence ID" value="XM_017475616.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RMD1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000031088"/>
    <dbReference type="GeneID" id="108269650"/>
    <dbReference type="KEGG" id="ipu:108269650"/>
    <dbReference type="CTD" id="797157"/>
    <dbReference type="OrthoDB" id="9941925at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042923">
        <property type="term" value="F:neuropeptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004983">
        <property type="term" value="F:neuropeptide Y receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001402">
        <property type="entry name" value="Prolrel_pep_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24235">
        <property type="entry name" value="NEUROPEPTIDE Y RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24235:SF19">
        <property type="entry name" value="PROLACTIN RELEASING PEPTIDE RECEPTOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01018">
        <property type="entry name" value="PRPRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039927084" evidence="10">
        <location>
            <begin position="22"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="126"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="254"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="350"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="106"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017331105.2"/>
        </source>
    </evidence>
    <sequence length="427" mass="47973" checksum="D8DD9F202F4A2A39" modified="2023-06-28" version="2">MCVCVCVCVCVLAILQSAVQPQEDESSLSELIFGSSTVYLRCDQLDKRKGKAVVNMDSLLNGTWENTSFNISPPSLSSFNGLNLLMELKILFVPLYSMLVLVACCGNLFLLFLIGLNKKLHSTTNFLIGNLALADLVMCLFCVPLTVFYAFDERGWIFGNFMCRFVSLMQTATVFAAVLSLTAIAVDRYIVVAYPIRRRVGRHFCIRLVLSIWLCALVMSIPATLHTVYLDLSAAGHHMAVCEEFWQEQELGRLIYSCFFLLLSYFVPLAAVSISYCAISCHLQHRATQGLIAAIASNQEKWGRKRRKTFRLLLISVLCFAFSWLPLQVVNLIRDLDTDFVILSKSHVNVIQVSCHLLAMSSACFNPFIYASLHDKFLSYLCRHMFQRRRQSQAQSSCTTSSRLPRQHTSSTVAGIPMAVTKTLQDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RN51</accession>
    <name>A0A2D0RN51_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Neuroblast differentiation-associated protein AHNAK</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">si:ch211-125o16.4</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017331939.2">
        <property type="nucleotide sequence ID" value="XM_017476450.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RN51"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027121"/>
    <dbReference type="GeneID" id="108270109"/>
    <dbReference type="KEGG" id="ipu:108270109"/>
    <dbReference type="OrthoDB" id="447516at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043034">
        <property type="term" value="C:costamere"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052082">
        <property type="entry name" value="Myelin_sheath_structural"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23348:SF41">
        <property type="entry name" value="NEUROBLAST DIFFERENTIATION-ASSOCIATED PROTEIN AHNAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23348">
        <property type="entry name" value="PERIAXIN/AHNAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="279"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="355"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="464"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="551"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1006"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1182"/>
            <end position="1224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017331939.2"/>
        </source>
    </evidence>
    <sequence length="1246" mass="132132" checksum="87E829C9CEAD8807" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RNI2</accession>
    <name>A0A2D0RNI2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein FAM98B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">fam98b</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM98 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017332063.1">
        <property type="nucleotide sequence ID" value="XM_017476574.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RNI2"/>
    <dbReference type="GeneID" id="108270168"/>
    <dbReference type="KEGG" id="ipu:108270168"/>
    <dbReference type="CTD" id="283742"/>
    <dbReference type="OrthoDB" id="512356at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072669">
        <property type="term" value="C:tRNA-splicing ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018797">
        <property type="entry name" value="FAM98"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31353">
        <property type="entry name" value="FAM98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31353:SF11">
        <property type="entry name" value="PROTEIN FAM98B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10239">
        <property type="entry name" value="DUF2465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01228">
        <property type="entry name" value="EGGSHELL"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="292"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="320"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017332063.1"/>
        </source>
    </evidence>
    <sequence length="400" mass="43449" checksum="E7BE0876CB1E32A5" modified="2017-12-20" version="1">MECDILDGLDQVGPYCLFHSYSGSLDEKTLSAACEQGFNSQEFAHVCTWLSSKLQHCDTKDQKSEIPDDPEKAKHELSKLLKELFCPYKGLDGELKTKKEHLKILLFLVSELQAMQIITSKTVKDTKIEKKISPLQDLQAICKVLKLPEPDQQQTTDLFSSIENEVKVLLKKVPEKHTGKPVLKESLNSEQWRQLEKINGVLLADYECRRRMLIKRLDVTVQSFSWSDRAKAKTDTMARAYQPKRHSLTLKSSISLAHLLAAREDICHMVKTSSESCRERTACAVNKVLMGRVPDRGGRPSEIEAPPPEMPPWQKRQDRGGGGGGYRGGGYGGGGGRGGGGGGYGGGGGGYGGGGHRGGYGGGGHRSGGHGSSGQGGWGRDGGGGRNQGGRGFKGGYYQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RNJ9</accession>
    <name>A0A2D0RNJ9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Molybdenum cofactor biosynthesis protein 1</fullName>
            <ecNumber evidence="6">4.1.99.22</ecNumber>
            <ecNumber evidence="7">4.6.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">mocs1</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Isoform MOCS1A and isoform MOCS1B probably form a complex that catalyzes the conversion of 5'-GTP to cyclic pyranopterin monophosphate (cPMP). MOCS1A catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate and MOCS1B catalyzes the subsequent conversion of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate to cPMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:49580"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59648"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.6.1.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>GTP + AH2 + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49576"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.1.99.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Isoform MOCS1A and isoform MOCS1B probably form a heterooligomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the MoaC family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the radical SAM superfamily. MoaA family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.22" evidence="6"/>
    <dbReference type="EC" id="4.6.1.17" evidence="7"/>
    <dbReference type="RefSeq" id="XP_017331535.1">
        <property type="nucleotide sequence ID" value="XM_017476046.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RNJ9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000014785"/>
    <dbReference type="GeneID" id="108269907"/>
    <dbReference type="KEGG" id="ipu:108269907"/>
    <dbReference type="CTD" id="4337"/>
    <dbReference type="OrthoDB" id="429626at2759"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061798">
        <property type="term" value="F:GTP 3',8'-cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01420">
        <property type="entry name" value="MoaC_PE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21117">
        <property type="entry name" value="Twitch_MoaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000117">
        <property type="entry name" value="molybdenum cofactor biosynthesis protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.640:FF:000002">
        <property type="entry name" value="Molybdenum cofactor biosynthesis protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.640">
        <property type="entry name" value="Molybdopterin cofactor biosynthesis C (MoaC) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01225_B">
        <property type="entry name" value="MoaA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01224_B">
        <property type="entry name" value="MoaC_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013483">
        <property type="entry name" value="MoaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040064">
        <property type="entry name" value="MoaA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010505">
        <property type="entry name" value="MoaA_twitch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023045">
        <property type="entry name" value="MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047594">
        <property type="entry name" value="MoaC_bact/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036522">
        <property type="entry name" value="MoaC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050105">
        <property type="entry name" value="MoCo_biosynth_MoaA/MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002820">
        <property type="entry name" value="Mopterin_CF_biosynth-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02666">
        <property type="entry name" value="moaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00581">
        <property type="entry name" value="moaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006870">
        <property type="entry name" value="PRK09364.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960:SF0">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SYNTHESIS PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01967">
        <property type="entry name" value="MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06463">
        <property type="entry name" value="Mob_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01383">
        <property type="entry name" value="cyclic_pyranopterin_phosphate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55040">
        <property type="entry name" value="Molybdenum cofactor biosynthesis protein C, MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="17" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="21">
        <location>
            <begin position="72"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00015273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00054222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00063038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017331535.1"/>
        </source>
    </evidence>
    <sequence length="730" mass="81155" checksum="A69D478CF2D08F22" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RUP9</accession>
    <name>A0A2D0RUP9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Phosphodiesterase</fullName>
            <ecNumber evidence="21">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">pde1ca</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">SDA103</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>3',5'-cyclic AMP + H2O = AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25277"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:25278"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>3',5'-cyclic GMP + H2O = GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16957"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:16958"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a nucleoside 3',5'-cyclic phosphate + H2O = a nucleoside 5'-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14653"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57867"/>
            <dbReference type="ChEBI" id="CHEBI:58464"/>
            <dbReference type="EC" id="3.1.4.17"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:14654"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="21">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="21">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="21"/>
    <dbReference type="RefSeq" id="XP_017333906.1">
        <property type="nucleotide sequence ID" value="XM_017478417.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RUP9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018153"/>
    <dbReference type="GeneID" id="108271107"/>
    <dbReference type="KEGG" id="ipu:108271107"/>
    <dbReference type="CTD" id="101883995"/>
    <dbReference type="OrthoDB" id="189220at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047555">
        <property type="term" value="F:3',5'-cyclic-GMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HD/PDEase_dom"/>
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    <dbReference type="InterPro" id="IPR013706">
        <property type="entry name" value="PDE1_N"/>
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        <property type="entry name" value="PDEase"/>
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        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
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        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
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        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08499">
        <property type="entry name" value="PDEase_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
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        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="13" id="KW-0114">cAMP</keyword>
    <keyword evidence="6" id="KW-0140">cGMP</keyword>
    <keyword evidence="8 21" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0458">Lysosome</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 20 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDEase" evidence="23">
        <location>
            <begin position="161"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="477"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="526"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="676"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="492"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="526"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="649"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="684"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="18">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="238"/>
            <end position="242"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
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    <feature type="binding site" evidence="19">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
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    <feature type="binding site" evidence="20">
        <location>
            <position position="386"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
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    <feature type="binding site" evidence="19">
        <location>
            <position position="437"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00033709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_017333906.1"/>
        </source>
    </evidence>
    <sequence length="712" mass="81671" checksum="217206672116E3D5" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RW13</accession>
    <name>A0A2D0RW13_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Signal peptide, CUB and EGF-like domain-containing protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Signal peptide, CUB and EGF-like domain-containing protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">scube2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="11">Forms homooligomers. Forms heterooligomers with SCUBE1 and SCUBE2. Interacts with TGFBR2 through the CUB domain; this interaction does not affect TGFB1-binding to TGFBR2. Interacts with BMP2, BMP4 and BMP7; the interaction is mediated by the CUB domain. Interacts with BMPR1A, BMPR1B and BMPR2; the interaction with BMPR1A and BMPR1B is BMP2- and BMP4-dependent.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell surface</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017334371.1">
        <property type="nucleotide sequence ID" value="XM_017478882.2"/>
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    <dbReference type="KEGG" id="ipu:108271338"/>
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    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
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    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045669">
        <property type="term" value="P:positive regulation of osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000032">
        <property type="entry name" value="signal peptide, CUB and EGF-like domain-containing protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000002">
        <property type="entry name" value="signal peptide, CUB and EGF-like domain-containing protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000199">
        <property type="entry name" value="signal peptide, CUB and EGF-like domain-containing protein 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000256">
        <property type="entry name" value="Signal peptide, CUB domain and EGF like domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000028">
        <property type="entry name" value="Signal peptide, CUB domain and EGF-like domain-containing 2"/>
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    <dbReference type="FunFam" id="2.10.25.10:FF:000030">
        <property type="entry name" value="Signal peptide, CUB domain and EGF-like domain-containing 2"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000035">
        <property type="entry name" value="Signal peptide, CUB domain and EGF-like domain-containing 2"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000002">
        <property type="entry name" value="Signal peptide, CUB domain and EGF-like domain-containing 2"/>
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    </dbReference>
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        <property type="entry name" value="Signal peptide, CUB domain and EGF-like domain-containing 3"/>
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    </dbReference>
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        <property type="entry name" value="Signal peptide, CUB domain, EGF-like 2"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000032">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000240">
        <property type="entry name" value="Vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024731">
        <property type="entry name" value="NELL2-like_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052071">
        <property type="entry name" value="SCUB_EGF-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011641">
        <property type="entry name" value="Tyr-kin_ephrin_A/B_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24046">
        <property type="entry name" value="SIGNAL PEPTIDE, CUB AND EGF-LIKE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24046:SF3">
        <property type="entry name" value="SIGNAL PEPTIDE, CUB AND EGF-LIKE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12947">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07699">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 14" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012768001" description="Signal peptide, CUB and EGF-like domain-containing protein 2" evidence="15">
        <location>
            <begin position="25"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="31"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="114"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="318"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="359"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="398"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="16">
        <location>
            <begin position="845"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="363"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017334371.1"/>
        </source>
    </evidence>
    <sequence length="1033" mass="114142" checksum="D565934ACDB39B70" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RWP7</accession>
    <name>A0A2D0RWP7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Urocortin-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC108271721</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Suppresses food intake, delays gastric emptying and decreases heat-induced edema. Might represent an endogenous ligand for maintaining homeostasis after stress.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Binds with high affinity to CRF receptors 2-alpha and 2-beta.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017334924.1">
        <property type="nucleotide sequence ID" value="XM_017479435.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RWP7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000028470"/>
    <dbReference type="GeneID" id="108271721"/>
    <dbReference type="KEGG" id="ipu:108271721"/>
    <dbReference type="OrthoDB" id="9949770at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051431">
        <property type="term" value="F:corticotropin-releasing hormone receptor 2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031669">
        <property type="term" value="P:cellular response to nutrient levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007586">
        <property type="term" value="P:digestion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000187">
        <property type="entry name" value="CRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024270">
        <property type="entry name" value="Urocortin_II/III"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17575">
        <property type="entry name" value="UROCORTIN-2 AND 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17575:SF1">
        <property type="entry name" value="UROCORTIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00473">
        <property type="entry name" value="CRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0372">Hormone</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012564916" evidence="9">
        <location>
            <begin position="23"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Corticotropin-releasing factor" evidence="10">
        <location>
            <begin position="87"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017334924.1"/>
        </source>
    </evidence>
    <sequence length="124" mass="13895" checksum="2CA973E689B1577A" modified="2017-12-20" version="1">MRTTLMGFYLLLLLLLPYTSLSQRQSYNAVTDENDEERDKMFVDVLSRFGIESTQLSPKQRLEPRGVRTTSPMSSNPKRAMQGSRFALSLDVPTTILSVLIDMAKEHDMRAKAAANAALMARIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RZ52</accession>
    <name>A0A2D0RZ52_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ras-responsive element-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">rreb1a</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017335778.2">
        <property type="nucleotide sequence ID" value="XM_017480289.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000000662"/>
    <dbReference type="GeneID" id="108272103"/>
    <dbReference type="KEGG" id="ipu:108272103"/>
    <dbReference type="CTD" id="338237"/>
    <dbReference type="OrthoDB" id="3069995at2759"/>
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Ictalurus punctatus</name>
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            <taxon>Eukaryota</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        </source>
    </evidence>
    <sequence length="316" mass="36038" checksum="66FA9A0AB3D6E9B0" modified="2017-12-20" version="1">MADGWSSLLDSRCADWMSQLPPQLHDVPLWDLAIPGSHDTMTYCLDQHSAVLPSQPRILTALDHIIPCVIRPCIKKWATTQELSISSQLDSGIRFLDLRIAHKKEDSSWVFYFAHGIYSLLTVKEALTDVAHWLEHHTNEVVIISLSAFNDVSPVQHYNLIDFLIQLFGKKLCPKSETPSLNACWTHGYQVILSYADPSGAKHEELWPAWDYWWANESDPNLVISYLEHNKETGRPAGFFMAGINLTEDTRYVLSHLTQSMKSMTLQSYNMLMDWVKKQHPGSRKTSVNIICADFVGILRNEFCEIVIGLNIDPKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S433</accession>
    <name>A0A2D0S433_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphatidylcholine transfer protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">START domain-containing protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">StAR-related lipid transfer protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">pctp</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Interacts with ACOT13/THEM2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017337482.1">
        <property type="nucleotide sequence ID" value="XM_017481993.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S433"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032772"/>
    <dbReference type="GeneID" id="108273024"/>
    <dbReference type="KEGG" id="ipu:108273024"/>
    <dbReference type="CTD" id="58488"/>
    <dbReference type="OMA" id="DYVYMRE"/>
    <dbReference type="OrthoDB" id="1295045at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008525">
        <property type="term" value="F:phosphatidylcholine transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000017">
        <property type="entry name" value="Phosphatidylcholine transfer protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051213">
        <property type="entry name" value="START_lipid_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19308:SF39">
        <property type="entry name" value="PHOSPHATIDYLCHOLINE TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="START" evidence="12">
        <location>
            <begin position="26"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017337482.1"/>
        </source>
    </evidence>
    <sequence length="214" mass="24957" checksum="766D2E734EE368A0" modified="2017-12-20" version="1">MALQFTDADFQEAWKELDEPQLKGEWELFTETTGVKIYRLYDKETGLYEYKVIGTLSSCSPEDCADVYMDLAYRKQWDSYVKELHEKDYGGQKAIYWEVKYPFPLSNRDYVYIRERRDLQVNGRKIWVVLARSSDVSQCPERSGVIRVKDYKQTVAMETDGAQGTKVFMNYFDNPGGNLPTWLINWAAKSGVPGFLKDMQDACSKYANYCKNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S5E8</accession>
    <name>A0A2D0S5E8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Keratinocyte differentiation factor 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">kdf1b</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017337947.1">
        <property type="nucleotide sequence ID" value="XM_017482458.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S5E8"/>
    <dbReference type="GeneID" id="108273298"/>
    <dbReference type="KEGG" id="ipu:108273298"/>
    <dbReference type="CTD" id="101886060"/>
    <dbReference type="OMA" id="RIDDWED"/>
    <dbReference type="OrthoDB" id="8640515at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003334">
        <property type="term" value="P:keratinocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010482">
        <property type="term" value="P:regulation of epidermal cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028003">
        <property type="entry name" value="KDF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35085">
        <property type="entry name" value="KERATINOCYTE DIFFERENTIATION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35085:SF2">
        <property type="entry name" value="KERATINOCYTE DIFFERENTIATION FACTOR 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15551">
        <property type="entry name" value="DUF4656"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="335"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017337947.1"/>
        </source>
    </evidence>
    <sequence length="368" mass="41122" checksum="9EBA49A3DE5AF1C7" modified="2017-12-20" version="1">MSGDGKSFSQRWERAGRPEALSMHSSSIKSRPYEERCVDPISPETQYPGALHKVHQKDANGNTTEPETVRFLPGKTESTSGSCMCGPCVSVKGCRTFACSVLTCGLYRVCRRMPCLLSGETRDDEPEVLKAVTSVPDDKTDFYPDLCIGGVKVDPSALMEEHEPVSFSLPKIDLDSPTYLSGSKHFSDLQDEDFIYEGTDEDVDSLITRKLLEVFSEFEINELAKCTSDSMFLKRSQEISQIISDIVQERNIKEQEAECRLVREIIRISTRKSKKKVTVRPQELQRDSGNDTWRSSKRSSKKSSFNSMNDGSLKISEERADDIEARNLRNNSKQSYSFSFPAAHSASSTDNVTPITSDTPLLSHGVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S9I6</accession>
    <name>A0A2D0S9I6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">[heparan sulfate]-glucosamine N-sulfotransferase</fullName>
            <ecNumber evidence="5">2.8.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ndst2b</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Glycan metabolism; heparan sulfate biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism; heparin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sulfotransferase 1 family. NDST subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.8" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017338894.1">
        <property type="nucleotide sequence ID" value="XM_017483405.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S9I6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021503"/>
    <dbReference type="GeneID" id="108273828"/>
    <dbReference type="KEGG" id="ipu:108273828"/>
    <dbReference type="CTD" id="798226"/>
    <dbReference type="OrthoDB" id="8958249at2759"/>
    <dbReference type="UniPathway" id="UPA00756"/>
    <dbReference type="UniPathway" id="UPA00862"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019213">
        <property type="term" value="F:deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015016">
        <property type="term" value="F:heparan sulfate N-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015012">
        <property type="term" value="P:heparan sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030210">
        <property type="term" value="P:heparin proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000176">
        <property type="entry name" value="bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021930">
        <property type="entry name" value="Heparan_SO4_deacetylase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056793">
        <property type="entry name" value="HSNSD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037359">
        <property type="entry name" value="NST/OST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605:SF53">
        <property type="entry name" value="BIFUNCTIONAL HEPARAN SULFATE N-DEACETYLASE_N-SULFOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605">
        <property type="entry name" value="HEPARAN SULFATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12062">
        <property type="entry name" value="HSNSD-CE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25119">
        <property type="entry name" value="HSNSD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Heparan sulfate-N-deacetylase N-terminal" evidence="18">
        <location>
            <begin position="91"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Heparan sulphate-N-deacetylase deacetylase" evidence="17">
        <location>
            <begin position="316"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="16">
        <location>
            <begin position="610"/>
            <end position="860"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF12062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF25119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017338894.1"/>
        </source>
    </evidence>
    <sequence length="889" mass="102037" checksum="336E2E9CD75B2F16" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SGQ8</accession>
    <accession>A0A2D0SGS4</accession>
    <name>A0A2D0SGQ8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="17 18">Delta-type opioid receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17 18" type="primary">LOC108275540</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17 18">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017341908.1">
        <property type="nucleotide sequence ID" value="XM_017486419.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017341909.3">
        <property type="nucleotide sequence ID" value="XM_017486420.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SGQ8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032647"/>
    <dbReference type="GeneID" id="108275540"/>
    <dbReference type="KEGG" id="ipu:108275540"/>
    <dbReference type="GeneTree" id="ENSGT00940000160661"/>
    <dbReference type="OMA" id="VNICIWA"/>
    <dbReference type="OrthoDB" id="6076970at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042923">
        <property type="term" value="F:neuropeptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001626">
        <property type="term" value="F:nociceptin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019233">
        <property type="term" value="P:sensory perception of pain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000014">
        <property type="entry name" value="Kappa-type opioid receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001418">
        <property type="entry name" value="Opioid_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229">
        <property type="entry name" value="NEUROPEPTIDES RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229:SF11">
        <property type="entry name" value="NOCICEPTIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00384">
        <property type="entry name" value="OPIOIDR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9 13 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="205"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="290"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="59"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017341908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017341909.3"/>
        </source>
    </evidence>
    <sequence length="371" mass="41069" checksum="F40E2D41CF448735" modified="2017-12-20" version="1">MGLSDSLDSVGDELALHLLNDSLLSGNMSDLDEDDLLSTGVKTTVVALYCVVCVVGLVGNCLVMYVIIRYTKMKTATNIYIFNLALADALVLATLPFQVTDVLLGYWPFGLALCKMVVAIDYYNMFTSIFTLTIMSVNRYIAVCHPVHSLAVRTPQRAKLVNVAVWVLASAIGVPVMIMAQVEVESSDIECMVVLPDPRSYSEPVFGICVFLFSFLIPVVIISVCYGLMVRRLRSVRLLSGSRSKDRNLRRISQMVLAVVAAFVLCWMPIQVLTLVQTLIKVNLGGSQTTVAAMHFCIALGYANSSLNPVLYAFLDENFKRCFREFCVQTNTDVELEPQQRKSVRKEETEEQEFIGNGVVNEAGAIPRCEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SHC2</accession>
    <name>A0A2D0SHC2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein 800a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">znf800a</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017341670.1">
        <property type="nucleotide sequence ID" value="XM_017486181.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SHC2"/>
    <dbReference type="GeneID" id="108275400"/>
    <dbReference type="KEGG" id="ipu:108275400"/>
    <dbReference type="CTD" id="568719"/>
    <dbReference type="OMA" id="HITELHM"/>
    <dbReference type="OrthoDB" id="10066279at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041697">
        <property type="entry name" value="Znf-C2H2_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039149">
        <property type="entry name" value="ZNF800"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21020">
        <property type="entry name" value="ZINC FINGER PROTEIN 800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21020:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN 800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16622">
        <property type="entry name" value="zf-C2H2_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="257"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="318"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="389"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="485"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="698"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="173"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="359"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="439"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="573"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="718"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="206"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="218"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="590"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="622"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="653"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="680"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="722"/>
            <end position="737"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017341670.1"/>
        </source>
    </evidence>
    <sequence length="744" mass="84260" checksum="6A2261A118D883C8" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SHE5</accession>
    <name>A0A2D0SHE5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytosolic iron-sulfur assembly component 2A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">MIP18 family protein FAM96A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ciao2a</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Monomer and homodimer. Component of the CIA complex. Interacts with CIAO1. Interacts with IREB2. Interacts with APAF1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MIP18 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017341695.1">
        <property type="nucleotide sequence ID" value="XM_017486206.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SHE5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018369"/>
    <dbReference type="GeneID" id="108275413"/>
    <dbReference type="KEGG" id="ipu:108275413"/>
    <dbReference type="CTD" id="84191"/>
    <dbReference type="OMA" id="HELGYRN"/>
    <dbReference type="OrthoDB" id="2746at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.130:FF:000004">
        <property type="entry name" value="cytosolic iron-sulfur assembly component 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039796">
        <property type="entry name" value="MIP18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12377:SF2">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR ASSEMBLY COMPONENT 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12377">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR ASSEMBLY COMPONENT 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00062218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00074929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00082916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017341695.1"/>
        </source>
    </evidence>
    <sequence length="157" mass="17983" checksum="62964611A5A3F5CD" modified="2017-12-20" version="1">MEIISSVLNKVFQLSRLSSVNNVPRSQKMEEKALEVYDVIRTIRDPEKPNTLEELDVVTEKCVEVQDLGDDEYLIIIRFSPTVPHCSLATLIGLCLQVKLQRCLPFKHKLEIYITEGTHSTEEDINKQINDKERVAAAMENPNLREIVEQCVIESDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SM86</accession>
    <name>A0A2D0SM86_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">CXADR-like membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC108276559</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017343818.1">
        <property type="nucleotide sequence ID" value="XM_017488329.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SM86"/>
    <dbReference type="GeneID" id="108276559"/>
    <dbReference type="KEGG" id="ipu:108276559"/>
    <dbReference type="OMA" id="PSKPQCW"/>
    <dbReference type="OrthoDB" id="9446970at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042454">
        <property type="entry name" value="CLMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44783">
        <property type="entry name" value="CXADR-LIKE MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44783:SF1">
        <property type="entry name" value="CXADR-LIKE MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012429262" evidence="3">
        <location>
            <begin position="20"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="234"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="11"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="136"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017343818.1"/>
        </source>
    </evidence>
    <sequence length="387" mass="42038" checksum="3B2BAE53F586B4C6" modified="2017-12-20" version="1">MSATFGSLLLPLVLGVLQARGQTEMKRVVGDNATLPCHHQLWHADTSLLDIEWLLVKPNAKQKAVITYFAGRIYDPSEGEAGKLTLSGDYLKGDASLMISDLSLTDSGEYSCKVKNGGKYYWNTVNLIVLVKPSKPRCWMEGRLLEGSDVKMSCKSADGSDPIRYKWERVLDKGRYVGKLPPLALLDLKNPEIVTLRNLSRESAGVYKCTASNDVGEESCTVEVKIHYVRGMGVMAGAAVGVSLGVLLLVLIVWLVFRKKDKKKYEEEETPNEIREDAEAPKAKLMKPNSLSSSRSGSSRSGTSSTQSMVPSGAPRGHKLHPPPPSAGPRKDSVQPSTLPAMPPAYTQVAPKLPEPHSSPKLSPGNLARMGAMPVMIPAQTKAFQTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SP41</accession>
    <name>A0A2D0SP41_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Vacuolar protein sorting-associated protein 51 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">vps51</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in retrograde transport from early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of protein retrieval from endosomes to the TGN, acid hydrolase sorting, lysosome function, endosomal cholesterol traffic and autophagy. Acts as component of the EARP complex that is involved in endocytic recycling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the Golgi-associated retrograde protein (GARP) complex. Component of the endosome-associated retrograde protein (EARP) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
            <location evidence="3">trans-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Recycling endosome</location>
        </subcellularLocation>
        <text evidence="3">Localizes to the trans-Golgi network as part of the GARP complex, while it localizes to recycling endosomes as part of the EARP complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the VPS51 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017344469.1">
        <property type="nucleotide sequence ID" value="XM_017488980.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SP41"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000025971"/>
    <dbReference type="GeneID" id="108276898"/>
    <dbReference type="KEGG" id="ipu:108276898"/>
    <dbReference type="CTD" id="738"/>
    <dbReference type="OMA" id="DIICERG"/>
    <dbReference type="OrthoDB" id="203678at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990745">
        <property type="term" value="C:EARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007041">
        <property type="term" value="P:lysosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014812">
        <property type="entry name" value="Vps51"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15954">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 51 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15954:SF4">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 51 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08700">
        <property type="entry name" value="VPS51_Exo84_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0967">Endosome</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="3" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU368010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017344469.1"/>
        </source>
    </evidence>
    <sequence length="802" mass="90259" checksum="E3B57C2FF4024951" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SPE0</accession>
    <name>A0A2D0SPE0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Exonuclease mut-7 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">exd3</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4 5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017344150.1">
        <property type="nucleotide sequence ID" value="XM_017488661.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017344151.1">
        <property type="nucleotide sequence ID" value="XM_017488662.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SPE0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030116"/>
    <dbReference type="GeneID" id="108276757"/>
    <dbReference type="KEGG" id="ipu:108276757"/>
    <dbReference type="CTD" id="54932"/>
    <dbReference type="OMA" id="CSNWANR"/>
    <dbReference type="OrthoDB" id="18193at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000074">
        <property type="entry name" value="exonuclease mut-7 homolog isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052408">
        <property type="entry name" value="Exonuclease_MUT-7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002782">
        <property type="entry name" value="Mut7-C_RNAse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47765">
        <property type="entry name" value="3'-5' EXONUCLEASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47765:SF2">
        <property type="entry name" value="EXONUCLEASE MUT-7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01927">
        <property type="entry name" value="Mut7-C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3'-5' exonuclease" evidence="2">
        <location>
            <begin position="374"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="576"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017344150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017344151.1"/>
        </source>
    </evidence>
    <sequence length="874" mass="99706" checksum="A4E93299791DED10" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0ST80</accession>
    <name>A0A2D0ST80_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">LOW QUALITY PROTEIN: cytochrome P450 2D17-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC108277596</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017345899.3">
        <property type="nucleotide sequence ID" value="XM_017490410.3"/>
    </dbReference>
    <dbReference type="GeneID" id="108277596"/>
    <dbReference type="KEGG" id="ipu:108277596"/>
    <dbReference type="OrthoDB" id="2789670at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000238">
        <property type="entry name" value="Cytochrome P450 2A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF327">
        <property type="entry name" value="CYTOCHROME P450 2F2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017345899.3"/>
        </source>
    </evidence>
    <sequence length="226" mass="25638" checksum="E0D2F1097213FE36" modified="2023-06-28" version="3">MITAVFHQKVDGSSFTENQLLMHLLDLHFAGTDTTSNTLLTAFLYLITHSDVQEKCQQEIDTVLXGKAQASFEDRHNMPYTQAVIHESQRITNTVPLSVFHCTTRDTELMGYNIPKGTVIIPNLSSVLCEEGHWKFAHEFNPSNFLNEQGQFEKPEAFIPFSAGLRSCLGEGLACMELFLTFVTLLHRFQFIWPEDAGEPDFTPVYGITLTPKPYRMGVRLRQPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0STS0</accession>
    <name>A0A2D0STS0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Na(+)/citrate cotransporter isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">slc13a5b</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily.</text>
    </comment>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
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                <person name="Gao S."/>
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                <person name="Koren S."/>
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                <person name="Geng X."/>
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                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
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                <person name="Yuan Z."/>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
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                <person name="Gao S."/>
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                <person name="Koren S."/>
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                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
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                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
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                <person name="Kucuktas H."/>
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                <person name="Liu H."/>
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                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
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    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
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    <dbReference type="RefSeq" id="XP_017346477.1">
        <property type="nucleotide sequence ID" value="XM_017490988.3"/>
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        <property type="component" value="Chromosome 17"/>
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        <property type="term" value="C:cytosol"/>
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        <property type="term" value="C:postsynapse"/>
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        <property type="project" value="GOC"/>
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        <property type="term" value="F:GTPase activator activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:regulation of G protein-coupled receptor signaling pathway"/>
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    <dbReference type="GO" id="GO:0036465">
        <property type="term" value="P:synaptic vesicle recycling"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="ARF GTPase-activating protein GIT1 isoform 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ARF GTPase-activating protein GIT2 isoform 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.20.5.170:FF:000015">
        <property type="entry name" value="ARF GTPase-activating protein GIT2 isoform 1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin_rpt"/>
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    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047161">
        <property type="entry name" value="GIT-like"/>
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    <dbReference type="InterPro" id="IPR032352">
        <property type="entry name" value="GIT1/2_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022018">
        <property type="entry name" value="GIT1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013724">
        <property type="entry name" value="GIT_SHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46097:SF1">
        <property type="entry name" value="ARF GTPASE-ACTIVATING PROTEIN GIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46097">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE INTERACTING ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12205">
        <property type="entry name" value="GIT1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GIT_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08518">
        <property type="entry name" value="GIT_SHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
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    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00555">
        <property type="entry name" value="GIT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="12">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="166"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="363"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="504"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="558"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="440"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="380"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="402"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="511"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="599"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="624"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017346477.1"/>
        </source>
    </evidence>
    <sequence length="763" mass="83221" checksum="10CE863E048D6515" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SVA2</accession>
    <name>A0A2D0SVA2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">U2 snRNP-associated SURP motif-containing protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">140 kDa Ser/Arg-rich domain protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">U2-associated protein SR140</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">u2surp</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">Interacts with ERBB4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the splicing factor SR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017346614.1">
        <property type="nucleotide sequence ID" value="XM_017491125.3"/>
    </dbReference>
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    <dbReference type="STRING" id="7998.ENSIPUP00000011598"/>
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    <dbReference type="KEGG" id="ipu:108278012"/>
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    <dbReference type="OMA" id="FKSRVCN"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21370">
        <property type="entry name" value="cwf21_SR140"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12223">
        <property type="entry name" value="RRM_SR140"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.790:FF:000006">
        <property type="entry name" value="U2 snRNP-associated SURP motif-containing protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000013">
        <property type="entry name" value="U2 snRNP-associated SURP motif-containing protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000177">
        <property type="entry name" value="U2 snRNP-associated SURP motif-containing protein-like isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="6.10.140.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.790">
        <property type="entry name" value="Surp module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013170">
        <property type="entry name" value="mRNA_splic_Cwf21_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051485">
        <property type="entry name" value="SR-CTD_assoc_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047488">
        <property type="entry name" value="SR140_cwf21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035009">
        <property type="entry name" value="SR140_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000061">
        <property type="entry name" value="Surp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035967">
        <property type="entry name" value="SWAP/Surp_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140">
        <property type="entry name" value="RNA PROCESSING PROTEIN LD23810P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140:SF10">
        <property type="entry name" value="U2 SNRNP-ASSOCIATED SURP DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08312">
        <property type="entry name" value="cwf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01805">
        <property type="entry name" value="Surp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01115">
        <property type="entry name" value="cwf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00648">
        <property type="entry name" value="SWAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109905">
        <property type="entry name" value="Surp module (SWAP domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50128">
        <property type="entry name" value="SURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RRM" evidence="17">
        <location>
            <begin position="196"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="SURP motif" evidence="18">
        <location>
            <begin position="352"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="CID" evidence="19">
        <location>
            <begin position="459"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="299"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="429"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="708"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="781"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="126"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="439"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="717"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="736"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="747"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="803"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="878"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="918"/>
            <end position="954"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017346614.1"/>
        </source>
    </evidence>
    <sequence length="954" mass="108928" checksum="2FFAC77811C80D91" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SVV7</accession>
    <name>A0A2D0SVV7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15 16">Zinc finger and BTB domain-containing protein 16-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15 16" type="primary">zbtb32</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15 16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017346809.1">
        <property type="nucleotide sequence ID" value="XM_017491320.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017346810.1">
        <property type="nucleotide sequence ID" value="XM_017491321.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SVV7"/>
    <dbReference type="GeneID" id="108278134"/>
    <dbReference type="KEGG" id="ipu:108278134"/>
    <dbReference type="CTD" id="27033"/>
    <dbReference type="GeneTree" id="ENSGT00940000154616"/>
    <dbReference type="OMA" id="VQHEPQS"/>
    <dbReference type="OrthoDB" id="8922241at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000553">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002171">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF48">
        <property type="entry name" value="ZINC FINGER PROTEIN 771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="12">
        <location>
            <begin position="26"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="352"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="380"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="408"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="122"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="122"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="148"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="308"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017346809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017346810.1"/>
        </source>
    </evidence>
    <sequence length="479" mass="54239" checksum="8A2235C8ED5EE94F" modified="2017-12-20" version="1">MIRIHNPHPLPFLHQTEMFLREGTLCDTVLTVEGYTFKAHALVLSWASKTLERQLINQNPAKGYCCTVDGVSPQTVKQILDYVYSACVEVSIGDLPDLLRGAQHLEMQSLLEQCELQMGIQDRVKSREEESSTPKINGGECDSRKKIPSSNEVSLSSSLESPSLTHFDHERSHHVTSLSPPPQKPPLSITVASPTASREPISSTPSLAQTSSLHWPPVNPSRHMVLTYTDFMAFQHMVACSYPTPMYPFLQHSLQLQMPSSLVGYTGLLHPHQHLLRPRPLALDRPLVPEVKQKTDLISAVLPGAASQERERKTKVQSDKESHCRHCSKPMVENSWRQSSRSASTGLEELLHECKFCQRDMREKRSLRSLRRGHGGEKPYQCKHCNKRFSLKHQLDTHLRVHTGEKPFECRLCGQRSRDYSAMIKHLRTHGGATPYQCTLCLEFCSSLVTMQKHLKSHPSQEFPPNWSLSSTYLYTCHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SWD4</accession>
    <name>A0A2D0SWD4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Solute carrier family 12 member 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC108278164</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) + chloride(in) = K(+)(out) + chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:72427"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the SLC12A transporter family. K/Cl co-transporter subfamily.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017491347.3"/>
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    <dbReference type="STRING" id="7998.ENSIPUP00000029487"/>
    <dbReference type="GeneID" id="108278164"/>
    <dbReference type="KEGG" id="ipu:108278164"/>
    <dbReference type="OrthoDB" id="2020542at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015379">
        <property type="term" value="F:potassium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006884">
        <property type="term" value="P:cell volume homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055064">
        <property type="term" value="P:chloride ion homeostasis"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055075">
        <property type="term" value="P:potassium ion homeostasis"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Solute carrier family 12 (potassium/chloride transporter), member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterized protein, isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000076">
        <property type="entry name" value="KCL_cotranspt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018491">
        <property type="entry name" value="SLC12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004842">
        <property type="entry name" value="SLC12A_fam"/>
    </dbReference>
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        <property type="entry name" value="2a30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827:SF47">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12 MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03522">
        <property type="entry name" value="SLC12"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01081">
        <property type="entry name" value="KCLTRNSPORT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0868">Chloride</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0769">Symport</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="443"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="489"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="543"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="567"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="600"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="18">
        <location>
            <begin position="113"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="18">
        <location>
            <begin position="400"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="19">
        <location>
            <begin position="696"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="19">
        <location>
            <begin position="826"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="19">
        <location>
            <begin position="1041"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00046331"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF03522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017346836.1"/>
        </source>
    </evidence>
    <sequence length="1109" mass="122970" checksum="94DA28368CE09D65" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SYC2</accession>
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        <submittedName>
            <fullName evidence="4">Protein c-Fos</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">fosl1b</name>
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    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
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    <reference evidence="4" key="2">
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                <consortium name="RefSeq"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
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        <property type="component" value="Chromosome 18"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
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        <property type="entry name" value="bZIP_sf"/>
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        <property type="entry name" value="FOS TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23351:SF25">
        <property type="entry name" value="FOS-RELATED ANTIGEN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="LEUZIPPRFOS"/>
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        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="100"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="142"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017347331.1"/>
        </source>
    </evidence>
    <sequence length="196" mass="22270" checksum="CA97385A84229428" modified="2017-12-20" version="1">MYQSHGSAAFTYGLFTDMAARLELNTTTQMHKKITDAVTSSSNADSVTSRPDDEWLLESSLVSEAECIWDTMASFLPSSLPNCPCLLSQCFNPELLHSATKGRSQDPDKEHSNQPKSIEECERRRARRERNRIAAARCRDRRRMLTDTLQNETEQLERVKAQLEAEIAGLEREKERLELVLEAHKPVCKMDGSNPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SYW1</accession>
    <name>A0A2D0SYW1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4 5">Plasminogen receptor (KT)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4 5" type="primary">plgrkt</name>
        <name evidence="3 4 5" type="synonym">CI046</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3 4 5" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4 5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="NP_001188089.2">
        <property type="nucleotide sequence ID" value="NM_001201160.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017347857.1">
        <property type="nucleotide sequence ID" value="XM_017492368.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017347859.1">
        <property type="nucleotide sequence ID" value="XM_017492370.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SYW1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000025595"/>
    <dbReference type="GeneID" id="108278784"/>
    <dbReference type="KEGG" id="ipu:108278784"/>
    <dbReference type="CTD" id="55848"/>
    <dbReference type="GeneTree" id="ENSGT00390000000375"/>
    <dbReference type="OMA" id="MGYYTDW"/>
    <dbReference type="OrthoDB" id="10256697at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010756">
        <property type="term" value="P:positive regulation of plasminogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019319">
        <property type="entry name" value="Plg-R(KT)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13411">
        <property type="entry name" value="PLASMINOGEN RECEPTOR (KT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13411:SF6">
        <property type="entry name" value="PLASMINOGEN RECEPTOR (KT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10166">
        <property type="entry name" value="DUF2368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 4 5" id="KW-0675">Receptor</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="NP_001188089.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017347857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017347859.1"/>
        </source>
    </evidence>
    <sequence length="149" mass="17161" checksum="F878C97A3E4339F7" modified="2017-12-20" version="1">MGILVSTVMDQNMKKQQEFMLHNARIQMERQILMQNEMRERQMAMQIAWSREFLKYFGSFFGLAAVGLTAGAIKRRKPGLFAPLVPLSFILAYQMDMAYGSYVHRVREEAESIMVTEADRLNLPHGPPTFESIEKARRAKSHLPPLSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T1W3</accession>
    <name>A0A2D0T1W3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ribonucleoside-diphosphate reductase</fullName>
            <ecNumber evidence="11">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">rrm1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 11">Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + [thioredoxin]-disulfide + H2O = a ribonucleoside 5'-diphosphate + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the ribonucleoside diphosphate reductase large chain family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="11"/>
    <dbReference type="RefSeq" id="XP_017348781.1">
        <property type="nucleotide sequence ID" value="XM_017493292.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T1W3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000036398"/>
    <dbReference type="GeneID" id="108279224"/>
    <dbReference type="KEGG" id="ipu:108279224"/>
    <dbReference type="CTD" id="6240"/>
    <dbReference type="OrthoDB" id="3000483at2759"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005971">
        <property type="term" value="C:ribonucleoside-diphosphate reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009265">
        <property type="term" value="P:2'-deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006264">
        <property type="term" value="P:mitochondrial DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070318">
        <property type="term" value="P:positive regulation of G0 to G1 transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009185">
        <property type="term" value="P:ribonucleoside diphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01679">
        <property type="entry name" value="RNR_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.70.20:FF:000001">
        <property type="entry name" value="Ribonucleoside-diphosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013346">
        <property type="entry name" value="NrdE_NrdA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013509">
        <property type="entry name" value="RNR_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008926">
        <property type="entry name" value="RNR_R1-su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039718">
        <property type="entry name" value="Rrm1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02506">
        <property type="entry name" value="NrdE_NrdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573:SF6">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48168">
        <property type="entry name" value="R1 subunit of ribonucleotide reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00089">
        <property type="entry name" value="RIBORED_LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="4 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0215">Deoxyribonucleotide synthesis</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="12">
        <location>
            <begin position="43"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017348781.1"/>
        </source>
    </evidence>
    <sequence length="840" mass="95451" checksum="6A669B8A35BAA81B" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T243</accession>
    <name>A0A2D0T243_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">O-acyltransferase like protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">oacyl</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017348987.1">
        <property type="nucleotide sequence ID" value="XM_017493498.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T243"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000024238"/>
    <dbReference type="GeneID" id="108279328"/>
    <dbReference type="CTD" id="319888"/>
    <dbReference type="OMA" id="QGQYCKL"/>
    <dbReference type="OrthoDB" id="118951at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002656">
        <property type="entry name" value="Acyl_transf_3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006621">
        <property type="entry name" value="Nose-resist-to-fluoxetine_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052728">
        <property type="entry name" value="O2_lipid_transport_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161">
        <property type="entry name" value="O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11161:SF0">
        <property type="entry name" value="O-ACYLTRANSFERASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01757">
        <property type="entry name" value="Acyl_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20146">
        <property type="entry name" value="NRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00703">
        <property type="entry name" value="NRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="176"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="390"/>
            <end position="412"/>
        </location>
    </feature>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="653"/>
            <end position="675"/>
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    </feature>
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            <begin position="40"/>
            <end position="157"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00703"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017348987.1"/>
        </source>
    </evidence>
    <sequence length="687" mass="77005" checksum="A324FCF574F4EAF5" modified="2017-12-20" version="1"> </sequence>
</entry>
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        <submittedName>
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        <name evidence="8" type="primary">mcrip1</name>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MCRIP family.</text>
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    <dbReference type="RefSeq" id="XP_017348735.1">
        <property type="nucleotide sequence ID" value="XM_017493246.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T2C5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013888"/>
    <dbReference type="GeneID" id="108279196"/>
    <dbReference type="KEGG" id="ipu:108279196"/>
    <dbReference type="CTD" id="348262"/>
    <dbReference type="OMA" id="PQNHERN"/>
    <dbReference type="OrthoDB" id="8170061at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="FAM195"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
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        <location>
            <begin position="1"/>
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        </location>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
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        </location>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010821"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017348735.1"/>
        </source>
    </evidence>
    <sequence length="94" mass="10814" checksum="D9AA4D32C33CAEA4" modified="2017-12-20" version="1">MTSSSTPRMHTYKRTSSPRSPTNTGELYTPAHEENVRFIHDTWLCVLRDVKSPHNSERGPQEYVEKNPNPNLLSFTPVDLSELRRPSTQDCKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T3Q3</accession>
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        <submittedName>
            <fullName evidence="4">DEP domain-containing protein 4</fullName>
        </submittedName>
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        <name evidence="4" type="primary">depdc4</name>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
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                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
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                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
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                <person name="Gao S."/>
                <person name="Li N."/>
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                <person name="Jiang Y."/>
                <person name="Zimin A."/>
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                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
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                <person name="Wang X."/>
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                <person name="Yuan Z."/>
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                <person name="Qin Z."/>
                <person name="Dunham R."/>
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                <person name="Sonstegard T."/>
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                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
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                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
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    <dbReference type="STRING" id="7998.ENSIPUP00000010504"/>
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    <dbReference type="OMA" id="LMQNVVF"/>
    <dbReference type="OrthoDB" id="276323at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="DEP DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16206:SF10">
        <property type="entry name" value="DEP DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEP" evidence="2">
        <location>
            <begin position="43"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017349225.1"/>
        </source>
    </evidence>
    <sequence length="539" mass="61724" checksum="A22CEF0C995EF973" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T866</accession>
    <name>A0A2D0T866_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13 14 15">Muscleblind-like protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13 14 15 16 17 18" type="primary">mbnl1</name>
    </gene>
    <organism evidence="12 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13 14 15" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13 14 15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the muscleblind family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017351073.1">
        <property type="nucleotide sequence ID" value="XM_017495584.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017351075.1">
        <property type="nucleotide sequence ID" value="XM_017495586.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017351076.1">
        <property type="nucleotide sequence ID" value="XM_017495587.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017351077.1">
        <property type="nucleotide sequence ID" value="XM_017495588.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017351078.1">
        <property type="nucleotide sequence ID" value="XM_017495589.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017351079.1">
        <property type="nucleotide sequence ID" value="XM_017495590.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T866"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000014637"/>
    <dbReference type="GeneID" id="108280526"/>
    <dbReference type="KEGG" id="ipu:108280526"/>
    <dbReference type="CTD" id="4154"/>
    <dbReference type="OrthoDB" id="6285980at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.210:FF:000004">
        <property type="entry name" value="Muscleblind like splicing regulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.210:FF:000002">
        <property type="entry name" value="Muscleblind-like 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.210">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054429">
        <property type="entry name" value="Znf-CCCH_Muscleblind-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12675">
        <property type="entry name" value="MUSCLEBLIND-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12675:SF7">
        <property type="entry name" value="MUSCLEBLIND-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22628">
        <property type="entry name" value="zf-CCCH_10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14608">
        <property type="entry name" value="zf-CCCH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="13"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="47"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="189"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="225"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="13"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="47"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="189"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="225"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017351073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017351075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017351076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017351077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017351078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017351079.1"/>
        </source>
    </evidence>
    <sequence length="415" mass="44874" checksum="F2E16FA13BD9F75F" modified="2017-12-20" version="1">MAMNMAHIRDPKWLTLEVCREFQRGTCSRSDAECKFAHPAKSCQVENGRVIACFDSLKGRCSRENCKYLHPPPHLKTQLEINGRNNLIQQKNMAMLAQQMQIASAIMPGSQLQPMSFFSVTPNLATNASAAAAAAAATFNPYLSPVSPALMPTAADILPSAPVLIGGNPAAVAPVTSTANTAAQKLLRTDRLEVCREYQRGNCSRGETDCRFAHPADSTMIDSSENTVTVCMDYIKGRCSRDKCKYFHPPAHLQAKIKAAQHQVNQATATAAMTAVKSLKRPLEATFDLQGLPHSLIPPLPKRPALEKTNGATTMFNAGMLQYQQALANMQFQQQFIPSGSILCMTPATSMVPMMHGASPAAVSAATTSATTVPFASASANQDSSLSKLTTNEYMQLIPIISAEHLTSHKYLTQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T8L9</accession>
    <name>A0A2D0T8L9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 15">Transferrin receptor protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC108280488</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Cell membrane</location>
            <topology evidence="15">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Melanosome</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="15">Stearoylated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 15">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017351000.1">
        <property type="nucleotide sequence ID" value="XM_017495511.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T8L9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000016718"/>
    <dbReference type="GeneID" id="108280488"/>
    <dbReference type="KEGG" id="ipu:108280488"/>
    <dbReference type="OMA" id="RIIDPIY"/>
    <dbReference type="OrthoDB" id="5841748at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004998">
        <property type="term" value="F:transferrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033572">
        <property type="term" value="P:transferrin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09848">
        <property type="entry name" value="M28_TfR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02128">
        <property type="entry name" value="PA_TfR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000065">
        <property type="entry name" value="Transferrin receptor 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.40">
        <property type="entry name" value="Transferrin receptor-like, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007365">
        <property type="entry name" value="TFR-like_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036757">
        <property type="entry name" value="TFR-like_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037324">
        <property type="entry name" value="TfR1/2_PA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF26">
        <property type="entry name" value="TRANSFERRIN RECEPTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04253">
        <property type="entry name" value="TFR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47672">
        <property type="entry name" value="Transferrin receptor-like dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 15" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="13 15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 15" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12 15 21" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="17">
        <location>
            <begin position="233"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="19">
        <location>
            <begin position="393"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin receptor-like dimerisation" evidence="18">
        <location>
            <begin position="667"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017351000.1"/>
        </source>
    </evidence>
    <sequence length="775" mass="84210" checksum="7871259ED2295B6E" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979E0M1</accession>
    <name>A0A979E0M1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Interferon a3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC108256863</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alpha/beta interferon family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047006488.2">
        <property type="nucleotide sequence ID" value="XM_047150532.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979E0M1"/>
    <dbReference type="GeneID" id="108256863"/>
    <dbReference type="KEGG" id="ipu:108256863"/>
    <dbReference type="OrthoDB" id="8695376at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005126">
        <property type="term" value="F:cytokine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000471">
        <property type="entry name" value="Interferon_alpha/beta/delta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11691:SF73">
        <property type="entry name" value="INTERFERON BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11691">
        <property type="entry name" value="TYPE I INTERFERON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00143">
        <property type="entry name" value="Interferon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039912892" evidence="8">
        <location>
            <begin position="23"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_047006488.2"/>
        </source>
    </evidence>
    <sequence length="192" mass="22623" checksum="2B12CD8D500AE969" modified="2023-06-28" version="2">MTVLNVWVFIVIALNSWNCARACAWMKTQNKATLSNFQVLSTNSIVHLQQACRDAGHDHGHLIMEFPNKQYNLITHLQANDHILFILRTLKGVMRIYRRKSKEMNCDQHKLRIFLLEVDRQILELERCARYIVTDEVHKRMFKEMEIHFRSLVSHLRNTEYNAKSWNDVAGVILKHLQRLDLLATTTKTILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979ER73</accession>
    <name>A0A979ER73_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Galectin-3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">lgals3a</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053535286.1">
        <property type="nucleotide sequence ID" value="XM_053679311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979ER73"/>
    <dbReference type="SMR" id="A0A979ER73"/>
    <dbReference type="GeneID" id="108262995"/>
    <dbReference type="CTD" id="557373"/>
    <dbReference type="OrthoDB" id="8112755at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001772">
        <property type="term" value="C:immunological synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048030">
        <property type="term" value="F:disaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019863">
        <property type="term" value="F:IgE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043236">
        <property type="term" value="F:laminin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048245">
        <property type="term" value="P:eosinophil chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048246">
        <property type="term" value="P:macrophage chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002548">
        <property type="term" value="P:monocyte chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045806">
        <property type="term" value="P:negative regulation of endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001237">
        <property type="term" value="P:negative regulation of extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030593">
        <property type="term" value="P:neutrophil chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050918">
        <property type="term" value="P:positive chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090280">
        <property type="term" value="P:positive regulation of calcium ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00070">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000023">
        <property type="entry name" value="Galectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044156">
        <property type="entry name" value="Galectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001079">
        <property type="entry name" value="Galectin_CRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346:SF179">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00337">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00908">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00276">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51304">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="48"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="243"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="60"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="145"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="170"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="220"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053535286.1"/>
        </source>
    </evidence>
    <sequence length="418" mass="42171" checksum="D8D2CDF140ADE0DB" modified="2023-02-22" version="1">MRQLSRTSDAPRGHFIGSLLLKTFSGGKLGCCHVMSEFSLADAIGDDAPSQASKTAHNRPSAPAGSSWPGAAPAGPAAPAWPGAAPNAPAGPAAPAWPGATPNAPAGPAAPAWPGATPSAPAGPAAPAWPGATPAWPGAPPSVPGAPAAPSWPGASPAGPAWPGAAPGGPNLPPTGGPVAPAPGAPGQFPSMPSAPGGYPTGPGMPGQFPAAPGQFPGMYPSPPGAPQAPYPNMPYQGNMYGPGGPGHFPPTTGYSGGAFPPLPSGAWGQPTGPYGGPSAPGGMLPVPYDLPLHAGIMPRLLITIVGEPVPGGQRFQVDFMKGEDVVFHFNPRFSEQTIVRNSNLSGYWGPEEREGGFPFIQGRQFELKILVEEDVYKVAVDGVHVLEYEHRSGGMDQVTLLRVYGDVTLYSVAPSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F1P7</accession>
    <name>A0A979F1P7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cAMP-dependent protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">prkacba</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.11" evidence="2"/>
    <dbReference type="RefSeq" id="XP_047014116.1">
        <property type="nucleotide sequence ID" value="XM_047158160.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979F1P7"/>
    <dbReference type="GeneID" id="108270713"/>
    <dbReference type="KEGG" id="ipu:108270713"/>
    <dbReference type="CTD" id="393998"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14209">
        <property type="entry name" value="STKc_PKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044109">
        <property type="entry name" value="STKc_PKA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF116">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0114">cAMP</keyword>
    <keyword evidence="7 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="87"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="15">
        <location>
            <begin position="342"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_047014116.1"/>
        </source>
    </evidence>
    <sequence length="394" mass="46022" checksum="7BC354D7A1D1AE05" modified="2023-02-22" version="1">MAHARDQNVGQSMGTNIALQKLDNFATRFFFNMHRKGKHDKESDGPHVSAYTHSWDERMKEFLAKAREDFLRKWECPPQSTTCLDDFERLKTLGTGSFGRVMLVKHKGTEQHYAMKILDKQKVVKLKQIEHTLNEKRILQAVSFPFLVRLEFSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEHHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYERQVEAPIIPKCRGPDDTSNFDDYEEEEIRISDTEKCAKEFLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R550</accession>
    <name>A0A9F7R550_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glutamate receptor-interacting protein 2a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">grip2a</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
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        <property type="nucleotide sequence ID" value="XM_053680435.1"/>
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    <dbReference type="GeneID" id="108265196"/>
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    <dbReference type="OrthoDB" id="75502at2759"/>
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        <property type="component" value="Chromosome 5"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098887">
        <property type="term" value="P:neurotransmitter receptor transport, endosome to postsynaptic membrane"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="PDZ1_GRIP1-2-like"/>
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        <property type="entry name" value="PDZ2_GRIP1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06684">
        <property type="entry name" value="PDZ3_GRIP1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06686">
        <property type="entry name" value="PDZ4_GRIP1-2-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PDZ5_GRIP1-2-like"/>
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        <property type="entry name" value="Glutamate receptor interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Glutamate receptor interacting protein 1"/>
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        <property type="entry name" value="Glutamate receptor interacting protein 1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Glutamate receptor interacting protein 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Glutamate receptor interacting protein 1"/>
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        <property type="entry name" value="PDZ"/>
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        <property type="entry name" value="GLUTAMATE RECEPTOR INTERACTING PROTEIN 2 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46227">
        <property type="entry name" value="GLUTAMATE RECEPTOR-INTERACTING PROTEIN GRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
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        <location>
            <begin position="89"/>
            <end position="172"/>
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            <begin position="406"/>
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        <location>
            <begin position="451"/>
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        <location>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50106"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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        <source>
            <dbReference type="RefSeq" id="XP_053536410.1"/>
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    <sequence length="1129" mass="121893" checksum="EF1BBE6325186388" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R5C1</accession>
    <name>A0A9F7R5C1_ICTPU</name>
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            <fullName evidence="7">Class I histocompatibility antigen, F10 alpha chain isoform X1</fullName>
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    </protein>
    <gene>
        <name evidence="7" type="primary">LOC108261330</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="7">Blood</tissue>
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        <text evidence="2">Belongs to the MHC class I family.</text>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_sub"/>
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    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
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    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
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        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
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        <property type="entry name" value="MHC CLASS I ANTIGEN TRANSCRIPT VARIANT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MHCCLASSI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039919010" evidence="4">
        <location>
            <begin position="24"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="340"/>
            <end position="364"/>
        </location>
    </feature>
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            <begin position="221"/>
            <end position="318"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053530398.1"/>
        </source>
    </evidence>
    <sequence length="404" mass="46174" checksum="DA5C163CCB91EFD3" modified="2023-06-28" version="1">MEHFSAVVKVFLLLRFSVHHSSADTHSLQYLYTGVTPGISFPEFTAVGLVDGELSEYYDSNIKNIIPKTEWIKEVDSDDPHYWYREKQVWLVEQEWLKNDVATAMKCFNQSKGVHTVQLMFGCELDDDGSTRAYSHFGYDGEDFISLDLKTKTWTAANVKAVIVENKWNPTGHEAEDCALYLKTECIYWLKKFVSYGRESLERKGFISETLCSLTLEAEAGDNVTIWCQHDLKHPEPIFWFKHTCDSVPFLLACKRYLVSAPSENCLFFTESERIVMSVHDKSTSLTITAVNVSDTGVYYCSFMKQKRVNFRNSTSLQVKGVNKTFSKNPDRAKGSDSSAVFFMLNAVFGAVIVILLCVLIIILKHRKTHGGVEAEDNEFSEKILKRRRDEVEYSYVVYSSVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R6M2</accession>
    <name>A0A9F7R6M2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Otoancorin isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">otoa</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mesothelin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053536451.1">
        <property type="nucleotide sequence ID" value="XM_053680476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R6M2"/>
    <dbReference type="GeneID" id="108266121"/>
    <dbReference type="CTD" id="146183"/>
    <dbReference type="OrthoDB" id="8195838at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010335">
        <property type="entry name" value="Mesothelin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026664">
        <property type="entry name" value="Stereocilin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048992">
        <property type="entry name" value="Stereocilin_LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23412:SF21">
        <property type="entry name" value="OTOANCORIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23412">
        <property type="entry name" value="STEREOCILIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06060">
        <property type="entry name" value="Mesothelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21058">
        <property type="entry name" value="Stereocilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Stereocilin LRR" evidence="7">
        <location>
            <begin position="357"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_053536451.1"/>
        </source>
    </evidence>
    <sequence length="1193" mass="130793" checksum="D79948A8E4F58606" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R7M6</accession>
    <name>A0A9F7R7M6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Lysine-specific demethylase</fullName>
            <ecNumber evidence="14">1.14.11.65</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">kdm3b</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>N(6),N(6)-dimethyl-L-lysyl(9)-[histone H3] + 2 2-oxoglutarate + 2 O2 = L-lysyl(9)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:60188"/>
            <dbReference type="Rhea" id="RHEA-COMP:15541"/>
            <dbReference type="Rhea" id="RHEA-COMP:15546"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
            <dbReference type="EC" id="1.14.11.65"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="14">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.</text>
    </comment>
    <comment type="domain">
        <text evidence="14">The JmjC domain and the C6-type zinc-finger are required for the demethylation activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12 14">Belongs to the JHDM2 histone demethylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.65" evidence="14"/>
    <dbReference type="RefSeq" id="XP_053538059.1">
        <property type="nucleotide sequence ID" value="XM_053682084.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108268841"/>
    <dbReference type="CTD" id="51780"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140683">
        <property type="term" value="F:histone H3K9me/H3K9me2 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070988">
        <property type="term" value="P:demethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000004">
        <property type="entry name" value="Putative lysine-specific demethylase 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054294">
        <property type="entry name" value="DUF7030"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054503">
        <property type="entry name" value="KDM3AB_Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045109">
        <property type="entry name" value="LSDs-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054504">
        <property type="entry name" value="PWWP_KDM3B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING HISTONE DEMETHYLATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549:SF8">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22989">
        <property type="entry name" value="DUF7030"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22988">
        <property type="entry name" value="PWWP_KDM3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22987">
        <property type="entry name" value="Tudor_KDM3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8 14" id="KW-0408">Iron</keyword>
    <keyword evidence="2 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039893425" description="Lysine-specific demethylase" evidence="16">
        <location>
            <begin position="26"/>
            <end position="1776"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="17">
        <location>
            <begin position="1513"/>
            <end position="1736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="248"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="610"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="712"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="822"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1144"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1196"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1291"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="252"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="324"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="619"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="716"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="823"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="854"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1158"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1200"/>
            <end position="1215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU369087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_053538059.1"/>
        </source>
    </evidence>
    <sequence length="1776" mass="190908" checksum="DDE7FE22A8D109D2" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RH11</accession>
    <name>A0A9F7RH11_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">LOW QUALITY PROTEIN: son of sevenless homolog 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">sos2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053535287.1">
        <property type="nucleotide sequence ID" value="XM_053679312.1"/>
    </dbReference>
    <dbReference type="GeneID" id="128631210"/>
    <dbReference type="KEGG" id="ipu:128631210"/>
    <dbReference type="CTD" id="6655"/>
    <dbReference type="OrthoDB" id="546434at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22914">
        <property type="entry name" value="HFD_SOS1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22915">
        <property type="entry name" value="HFD_SOS1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000029">
        <property type="entry name" value="son of sevenless homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000015">
        <property type="entry name" value="son of sevenless homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF150">
        <property type="entry name" value="SON OF SEVENLESS HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="6">
        <location>
            <begin position="169"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="412"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="7">
        <location>
            <begin position="562"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="5">
        <location>
            <begin position="748"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="989"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1015"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1043"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1109"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1214"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1047"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1119"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1168"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1220"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1291"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_053535287.1"/>
        </source>
    </evidence>
    <sequence length="1312" mass="150295" checksum="47E83526B3CFC832" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RRR0</accession>
    <name>A0A9F7RRR0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC108262003 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108262003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053542501.1">
        <property type="nucleotide sequence ID" value="XM_053686526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RRR0"/>
    <dbReference type="GeneID" id="108262003"/>
    <dbReference type="KEGG" id="ipu:108262003"/>
    <dbReference type="OrthoDB" id="8770287at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053542501.1"/>
        </source>
    </evidence>
    <sequence length="327" mass="36925" checksum="3F6F24B2DDB5EA39" modified="2023-06-28" version="1">MDASFYFYSRRLKLLIEDPVPYIKEGDPSASCLFINTCVPDSIIAANHTASIKDLTVEDLLNKDDFFNKLLSLLKEKKYIEARKHFVKELHPDKINLFGTVKDYLPLFDKLACAEITYNQNPPNDSAIYKEIPSILKKCGDVIVLGNPSDPTLLLVHRDVNNALYRDYKSTEPPLSVDDEKKRTFMLQFLLLGKEEHMTTCFSSFDGMWHLYDNDPRKPSFQPFDLESLKDYDICLAGYVNISQEQECKLGVAETGVGAKPHFSHLEKAQEDPSRNFYPQQSVVDVEMEDLSGTISYSVADTGGGRLGAKPFIEDTEMPSCSSSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RV51</accession>
    <name>A0A9F7RV51_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Antigen WC1.1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC128635383</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053544107.1">
        <property type="nucleotide sequence ID" value="XM_053688132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RV51"/>
    <dbReference type="GeneID" id="128635383"/>
    <dbReference type="KEGG" id="ipu:128635383"/>
    <dbReference type="OrthoDB" id="536948at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000012">
        <property type="entry name" value="CD163 molecule like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000002">
        <property type="entry name" value="Scavenger receptor cysteine-rich type 1 protein M130"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000004">
        <property type="entry name" value="Scavenger receptor cysteine-rich type 1 protein M130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000009">
        <property type="entry name" value="WC1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331:SF468">
        <property type="entry name" value="SCAVENGER RECEPTOR CYSTEINE-RICH TYPE 1 PROTEIN M160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331">
        <property type="entry name" value="SCAVENGER RECEPTOR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="SRCR" evidence="13">
        <location>
            <begin position="64"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="13">
        <location>
            <begin position="166"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="13">
        <location>
            <begin position="280"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="13">
        <location>
            <begin position="391"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="13">
        <location>
            <begin position="497"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="13">
        <location>
            <begin position="599"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="13">
        <location>
            <begin position="726"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="133"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="305"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="318"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="458"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="522"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="535"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="566"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="637"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="667"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="751"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="795"/>
            <end position="805"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_053544107.1"/>
        </source>
    </evidence>
    <sequence length="852" mass="92666" checksum="D61229C62DEEDD91" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TB31</accession>
    <name>A0A9F7TB31_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutaminase</fullName>
            <ecNumber evidence="2">3.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gls2b</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.2" evidence="2"/>
    <dbReference type="RefSeq" id="XP_053529964.1">
        <property type="nucleotide sequence ID" value="XM_053673989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TB31"/>
    <dbReference type="GeneID" id="108255189"/>
    <dbReference type="CTD" id="556445"/>
    <dbReference type="OrthoDB" id="9995210at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006543">
        <property type="term" value="P:L-glutamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.710.10:FF:000002">
        <property type="entry name" value="glutaminase kidney isoform, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00313">
        <property type="entry name" value="Glutaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015868">
        <property type="entry name" value="Glutaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041541">
        <property type="entry name" value="Glutaminase_EF-hand"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03814">
        <property type="entry name" value="Gln_ase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12544">
        <property type="entry name" value="GLUTAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12544:SF33">
        <property type="entry name" value="GLUTAMINASE LIVER ISOFORM, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17959">
        <property type="entry name" value="EF-hand_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04960">
        <property type="entry name" value="Glutaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Glutaminase EF-hand" evidence="8">
        <location>
            <begin position="83"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="481"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="481"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053529964.1"/>
        </source>
    </evidence>
    <sequence length="610" mass="67504" checksum="7C9F597DA9550E30" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TGM3</accession>
    <name>A0A9F7TGM3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein LOC108265261</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC108265261</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SDA103</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053536549.1">
        <property type="nucleotide sequence ID" value="XM_053680574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TGM3"/>
    <dbReference type="GeneID" id="108265261"/>
    <dbReference type="KEGG" id="ipu:108265261"/>
    <dbReference type="OrthoDB" id="8954574at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_053536549.1"/>
        </source>
    </evidence>
    <sequence length="226" mass="24775" checksum="032FA4D2ADCA0118" modified="2023-06-28" version="1">MSVGRQQRRMLAAPFRFGSAFLNTGLGLKIIISAVERSRVCAPVFDSIRGSVTINYVGKNKVMKKILNVGEETYEAQAQMIAPDRRIDISAVEGSNVFAPVTYNSTGTLIINYFVITKFTNIGDAKTEPHAQVIVPLRRLSSYPPSGLRINICAFDGSTVCSPVFHNCTGTLTINYFRLVGIPKMLKLAPEARPEGGVCRQMSGNRATHVSQPVGWQSCDEQSQMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7THC3</accession>
    <name>A0A9F7THC3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Germ cell-specific gene 1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC108275002</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GSG1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053533014.1">
        <property type="nucleotide sequence ID" value="XM_053677039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7THC3"/>
    <dbReference type="GeneID" id="108275002"/>
    <dbReference type="KEGG" id="ipu:108275002"/>
    <dbReference type="OrthoDB" id="10001768at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000037">
        <property type="entry name" value="GSG1 like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012478">
        <property type="entry name" value="GSG-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050579">
        <property type="entry name" value="PMP-22/EMP/MP20-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671">
        <property type="entry name" value="EPITHELIAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671:SF92">
        <property type="entry name" value="GERM CELL-SPECIFIC GENE 1-LIKE PROTEIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07803">
        <property type="entry name" value="GSG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039896617" evidence="7">
        <location>
            <begin position="26"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_053533014.1"/>
        </source>
    </evidence>
    <sequence length="249" mass="28193" checksum="71543753778097C3" modified="2023-06-28" version="1">MKLQNGPRATLAFTLNCLAFTLAVSAVTTSYWCSGTRKIVKPLCTGPEKVKQNYCIHFNSSDANNTHLVQYIYETGEEKFILKKFHTGIWFTCEQAVDLLGFTCRAFLEISPNHERGVLWLCIIGECLYITLLFIGVSLMAVEMCHCCNTMNRLKLNAFAALFTALSGLIGMVAHMMFTTAFQLAVLMGPENWKPKTWDYSWSYLLAWASFATCMASAVTALNHYTKTMIQFKFKRRNIENNLELLGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7THT5</accession>
    <name>A0A9F7THT5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tripartite motif-containing protein 2</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">E3 ubiquitin-protein ligase TRIM2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">RING-type E3 ubiquitin transferase TRIM2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25 26 27 28" type="primary">trim2a</name>
    </gene>
    <organism evidence="24 26">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">SDA103</strain>
        </source>
    </reference>
    <reference evidence="25 26 27" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25 26 27">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Forms homooligomers. Interacts with TRIM3; this interaction reduces TRIM2 activity. Interacts with myosin V; myosin V may not be a substrate for ubiquitination. Interacts with NEFL. Interacts with phosphorylated BCL2L11. Interacts with SIRPA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRIM/RBCC family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_053533400.1">
        <property type="nucleotide sequence ID" value="XM_053677425.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053533401.1">
        <property type="nucleotide sequence ID" value="XM_053677426.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053533402.1">
        <property type="nucleotide sequence ID" value="XM_053677427.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053533403.1">
        <property type="nucleotide sequence ID" value="XM_053677428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7THT5"/>
    <dbReference type="GeneID" id="108260592"/>
    <dbReference type="KEGG" id="ipu:108260592"/>
    <dbReference type="CTD" id="100003782"/>
    <dbReference type="OrthoDB" id="342730at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14960">
        <property type="entry name" value="NHL_TRIM2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000004">
        <property type="entry name" value="Tripartite motif containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000032">
        <property type="entry name" value="Tripartite motif containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003649">
        <property type="entry name" value="Bbox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001258">
        <property type="entry name" value="NHL_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050952">
        <property type="entry name" value="TRIM-NHL_E3_ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057750">
        <property type="entry name" value="TRIM2/3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24104">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NHLRC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24104:SF58">
        <property type="entry name" value="TRIPARTITE MOTIF-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01436">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00502">
        <property type="entry name" value="BBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51125">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="20" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="22">
        <location>
            <begin position="26"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="23">
        <location>
            <begin position="111"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="18">
        <location>
            <begin position="318"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="19">
        <location>
            <begin position="473"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="19">
        <location>
            <begin position="518"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="19">
        <location>
            <begin position="562"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="19">
        <location>
            <begin position="607"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="19">
        <location>
            <begin position="654"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="19">
        <location>
            <begin position="698"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="424"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00043214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_053533400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_053533401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_053533402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_053533403.1"/>
        </source>
    </evidence>
    <sequence length="742" mass="80740" checksum="794F514D78715109" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TIW0</accession>
    <name>A0A9F7TIW0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ankyrin repeat domain-containing protein 63</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC128633596</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053538814.1">
        <property type="nucleotide sequence ID" value="XM_053682839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TIW0"/>
    <dbReference type="GeneID" id="128633596"/>
    <dbReference type="KEGG" id="ipu:128633596"/>
    <dbReference type="OrthoDB" id="5406014at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24124">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24124:SF14">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_25, WHOLE GENOME SHOTGUN SEQUENCE"/>
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    </dbReference>
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        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="46"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="83"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053538814.1"/>
        </source>
    </evidence>
    <sequence length="512" mass="57550" checksum="AB11454898E56A08" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TKH4</accession>
    <name>A0A9F7TKH4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">tmtc4</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TMTC family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="5"/>
    <dbReference type="RefSeq" id="XP_053536657.1">
        <property type="nucleotide sequence ID" value="XM_053680682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TKH4"/>
    <dbReference type="GeneID" id="108266657"/>
    <dbReference type="CTD" id="84899"/>
    <dbReference type="OrthoDB" id="19588at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052346">
        <property type="entry name" value="O-mannosyl-transferase_TMTC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013618">
        <property type="entry name" value="TMTC_DUF1736"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44227">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44227:SF3">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE TMTC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08409">
        <property type="entry name" value="TMTC_DUF1736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 13" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="231"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="413"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="441"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1736" evidence="15">
        <location>
            <begin position="322"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="511"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="545"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="579"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="715"/>
            <end position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_053536657.1"/>
        </source>
    </evidence>
    <sequence length="770" mass="86118" checksum="9462B6ECA7C3F8A7" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TKH8</accession>
    <name>A0A9F7TKH8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein cfap97d2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cfap97d2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CFAP97 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053536672.1">
        <property type="nucleotide sequence ID" value="XM_053680697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TKH8"/>
    <dbReference type="GeneID" id="124628279"/>
    <dbReference type="KEGG" id="ipu:124628279"/>
    <dbReference type="CTD" id="101929355"/>
    <dbReference type="OrthoDB" id="2163395at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038792">
        <property type="entry name" value="CFAP97D1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029488">
        <property type="entry name" value="Hmw/CFAP97"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33768:SF7">
        <property type="entry name" value="CFAP97 DOMAIN CONTAINING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33768">
        <property type="entry name" value="MIP11318P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13879">
        <property type="entry name" value="Hmw_CFAP97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="145"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053536672.1"/>
        </source>
    </evidence>
    <sequence length="173" mass="20372" checksum="9264372451BDDB09" modified="2023-06-28" version="1">MQHKAHQPLLPCASKYLQYRWDKSGYEIHMKKLEKERLSKIQRENLMLLDKISHIKRNTGRTDCRNEYADKRLDSDKRQEALLEIAKDNKIMLRHLTQCAPYYSVQVWNEQWIKTLEIMKSVGRFPPLNHAQIVHPVAHDACLWTGGGNRSTQRKPPNHRENMQTPHRGGRGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TLX1</accession>
    <name>A0A9F7TLX1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Zinc finger protein OZF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">si:ch211-119o8.6</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053537739.1">
        <property type="nucleotide sequence ID" value="XM_053681764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TLX1"/>
    <dbReference type="GeneID" id="108268351"/>
    <dbReference type="KEGG" id="ipu:108268351"/>
    <dbReference type="OrthoDB" id="1095242at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000032">
        <property type="entry name" value="Krueppel-like factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001370">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001171">
        <property type="entry name" value="Zinc finger protein 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002401">
        <property type="entry name" value="zinc finger protein 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000202">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000188">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399:SF70">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="242"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="270"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="298"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="326"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="354"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="382"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="410"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="438"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_053537739.1"/>
        </source>
    </evidence>
    <sequence length="471" mass="54609" checksum="065C944DF9FD140B" modified="2023-06-28" version="1">MDPMDQKAQTLSNAPSFLMALRANLCHECGKSFESTSALKTHRKSHSVQWLYGCSTCGKQYTRKKELSLHQRLHTNESLLYCAYCKRKFTRKRDLICHERSHTGEKPFLCTLCGTSFSRRSTLKLHIQRHKGIKLYQCDQCGMSFHTSAALKIHKAIHTGEKPFACHQCDKRFVLKAHLDIHLHKHAGETPYSCSVCGKAFKWKQSLTTHQLTHQEQRPFKCPDCDCHLKSHQLIHSSETPYKCSHCGKAFKRRNSLKVYERIHTGKKPYSCDQCGKNFVSSSELSVHRRVHSDERNYKCSECEKTFRYMPSLKVHQRTHSMERPYHCRECGKSFIASSHLRTHEEMHKNEQRHHCTECGQGFNCSSTLKSHQMTHSTRTEFRCDDCGKIFIRKDTFKIHQQIHTGARVYNCSECGNSFSSSSNLQRHLQNHKVKTMYHCSECEKSFSSVAYLRKHQLAVHAAEAPYVCTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TQG0</accession>
    <name>A0A9F7TQG0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="2">General transcription factor II-I repeat domain-containing protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC128634645</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SDA103</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053541399.1">
        <property type="nucleotide sequence ID" value="XM_053685424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TQG0"/>
    <dbReference type="GeneID" id="128634645"/>
    <dbReference type="KEGG" id="ipu:128634645"/>
    <dbReference type="OrthoDB" id="1101576at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913">
        <property type="entry name" value="EPM2A-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913:SF9">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR II-I REPEAT DOMAIN-CONTAINING PROTEIN 2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_053541399.1"/>
        </source>
    </evidence>
    <sequence length="453" mass="51417" checksum="342C7D1CDB701390" modified="2023-06-28" version="1">MISLKGTTTGRDIFEAVSEAVEKMGLKWDKLCGVTTDGAPAMTGDRKGMASMVCAKVKESGGEAFRMHCIIHQEALCAKTVQLGDVMNTVVKTVNIIRARGLYHREFQAFLSEVDAEYGDVFYHSEVRWLSRGYVLQRFYSMRSEIDQFMKEKGRPLHEPSDPLWLADLAFLVDLTHHLNTLNKNLQGKEQLVPHLYAHMKAFCVKLCLFETQLRSFNAAHFPALSEIKSAFPKADLSAKKEKYVSVIASLVTEFNQRFQDFSVIEKQIKLFSTPFLVDAEEVEESLQLELIEMQCDDSLKSQHQLLSLFDFYQSLDSAKFPLMRRHAKRMMSLFGSTYICEQAFSLLNQNKSRLRTRMAEAISVKSFVSQPPNFLLTFQPSFNPKDSITAPTECNVLHIIADNLLSPEELVALQAAIDPKGPSPSQKSTWQPISTYSTYWANSKEQFHLSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TQP2</accession>
    <name>A0A9F7TQP2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">LOW QUALITY PROTEIN: actin-like protein 6B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">actl6b</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053542342.1">
        <property type="nucleotide sequence ID" value="XM_053686367.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108275708"/>
    <dbReference type="KEGG" id="ipu:108275708"/>
    <dbReference type="CTD" id="51412"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="CDD" id="cd13395">
        <property type="entry name" value="ASKHA_NBD_Arp4_ACTL6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039896250" evidence="2">
        <location>
            <begin position="22"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053542342.1"/>
        </source>
    </evidence>
    <sequence length="666" mass="73954" checksum="DD93FD53FE10B9B9" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3TEX6</accession>
    <name>E3TEX6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15 17">G-protein coupled receptor 183-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">GP83A</name>
        <name evidence="17" type="synonym">gp83a</name>
        <name evidence="17" type="synonym">gpr183</name>
        <name evidence="17" type="synonym">gpr183a</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 17" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="GU588909">
        <property type="protein sequence ID" value="ADO28862.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187593.1">
        <property type="nucleotide sequence ID" value="NM_001200664.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TEX6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020800"/>
    <dbReference type="GeneID" id="100528394"/>
    <dbReference type="KEGG" id="ipu:100528394"/>
    <dbReference type="CTD" id="556770"/>
    <dbReference type="OrthoDB" id="10021141at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008142">
        <property type="term" value="F:oxysterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15159">
        <property type="entry name" value="7tmA_EBI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000017">
        <property type="entry name" value="lysophosphatidic acid receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047160">
        <property type="entry name" value="GP183-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24237">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24237:SF7">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 183"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 12 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="110"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="196"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="283"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="48"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ADO28862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="NP_001187593.1"/>
        </source>
    </evidence>
    <sequence length="366" mass="41112" checksum="4EC4666BC61620F4" modified="2011-01-11" version="1">MAMMATPNNSSMAVSIAGNCTNLYDHRGWAHVLLPVVYTFIFVVGLLGNVLALHVIWPNMKKINSTTVYSANLVASDILFALALPLRVAYYGLGFHWPMGEGLCKATALLFYINMYAGVNFMTCLSVDRFIAVVLPLRFSCFRKIHNVRYICVGVWMLVLAQTLPLLSMPMTHEEHDGYITCMEYPNFEQVDGLPYILIGAAFLGYGVPLVTILVCYSALCSKLRELAKTNQLTEKSGLSRKAIGVICCVILVFVVCYSPYHIDLLQYMIRKLHYQPDCGELHAFQISLHATVSFMNLNSCLDPFIYFFACKGYKKKVMKLLKRQVSMSLSSMVKTSPDESSRDVDKITMGTRSCHRERSSMLMGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3TGC7</accession>
    <name>E3TGC7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphopantothenoylcysteine decarboxylase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">CoaC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">COAC</name>
        <name evidence="9" type="synonym">coac</name>
        <name evidence="9" type="synonym">ppcdc</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the decarboxylation of the cysteine moiety of 4-phosphopantothenoylcysteine to form 4'-phosphopantotheine and this reaction forms part of the biosynthesis of coenzyme A.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HFCD (homooligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GU589413">
        <property type="protein sequence ID" value="ADO29363.1"/>
        <property type="molecule type" value="mRNA"/>
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        <property type="nucleotide sequence ID" value="NM_001201033.1"/>
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    <dbReference type="AlphaFoldDB" id="E3TGC7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000031578"/>
    <dbReference type="GeneID" id="100528763"/>
    <dbReference type="KEGG" id="ipu:100528763"/>
    <dbReference type="CTD" id="60490"/>
    <dbReference type="GeneTree" id="ENSGT00440000038107"/>
    <dbReference type="OrthoDB" id="1532798at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071513">
        <property type="term" value="C:phosphopantothenoylcysteine decarboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1950:FF:000004">
        <property type="entry name" value="Phosphopantothenoylcysteine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359">
        <property type="entry name" value="HOMO-OLIGOMERIC FLAVIN CONTAINING CYS DECARBOXYLASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359:SF6">
        <property type="entry name" value="PHOSPHOPANTOTHENOYLCYSTEINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="6">
        <location>
            <begin position="24"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00056708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00070201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00082063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADO29363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="NP_001187962.1"/>
        </source>
    </evidence>
    <sequence length="205" mass="22417" checksum="FCFB5596931DA344" modified="2011-01-11" version="1">MQPTSDCTNVSSALNLSCTGSGFHVLLGVTGSVAALKLPLLVTQLLEIPGIEVRVVTTDHATHFYKVAEVPVRVYTDNDEWEMWKTRSDPVLHIELRRWADLLVIAPLDANTLGKIASGICDNLLTCIVRAWDMSRPLLFCPAMNTAMWQHPITAQQVATLQGFGYVEIPCISKKLVCGDEGKGAMAEVSTIVDAIQQYLPRPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3TGJ7</accession>
    <name>E3TGJ7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Insulin-induced gene protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">INSI2</name>
        <name evidence="15" type="synonym">insi2</name>
        <name evidence="15" type="synonym">insig2</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Mediates feedback control of cholesterol synthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the INSIG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="GU589483">
        <property type="protein sequence ID" value="ADO29433.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001188015.1">
        <property type="nucleotide sequence ID" value="NM_001201086.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TGJ7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019191"/>
    <dbReference type="GeneID" id="100528816"/>
    <dbReference type="KEGG" id="ipu:100528816"/>
    <dbReference type="CTD" id="51141"/>
    <dbReference type="OrthoDB" id="205546at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032937">
        <property type="term" value="C:SREBP-SCAP-Insig complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032869">
        <property type="term" value="P:cellular response to insulin stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006695">
        <property type="term" value="P:cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032933">
        <property type="term" value="P:SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036316">
        <property type="term" value="P:SREBP-SCAP complex retention in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025929">
        <property type="entry name" value="INSIG_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15301">
        <property type="entry name" value="INSULIN-INDUCED GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15301:SF10">
        <property type="entry name" value="INSULIN-INDUCED GENE 2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07281">
        <property type="entry name" value="INSIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3 12" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 12" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="10" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU241113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADO29433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="NP_001188015.1"/>
        </source>
    </evidence>
    <sequence length="224" mass="24639" checksum="2B4F4C678362CD1E" modified="2011-01-11" version="1">MAEVTSGSMTSRQGPYLSMVTNRTTNLLIRGAMLFGVGVFLALVLNLLQVQRNVTLFPPDVISSIFSSAWWVPPCCGTASALIGLLYPCIDKRFGEPHKLKREWSSVMGCVAVFVGINHASAKVDFANNVQLSLTLAALSVGLWWTFDRSRSGFGLGVAIAFLATLTTQLLVYNGVFQYTSPDFLYIRSWLPCIFFAGVITMGNIGRQLALYECKVVQEKIHQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2026-01-28" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>Q8SF45</accession>
    <name>Q8SF45_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">NADH-ubiquinone oxidoreductase chain 6</fullName>
            <ecNumber evidence="3 15">7.1.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17 24" type="primary">ND6</name>
        <name evidence="24" type="ORF">KEF82_p02</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <geneLocation type="mitochondrion" evidence="17 24"/>
    <reference evidence="24" key="1">
        <citation type="submission" date="2002-03" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <consortium name="NCBI Genome Project"/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">Norris</strain>
        </source>
    </reference>
    <reference evidence="17 23 24" key="2">
        <citation type="journal article" date="2003" name="DNA Seq." volume="14" first="265" last="277">
            <title>Complete sequence and characterization of the channel catfish mitochondrial genome.</title>
            <authorList>
                <person name="Waldbieser G.C."/>
                <person name="Bilodeau A.L."/>
                <person name="Nonneman D.J."/>
            </authorList>
            <dbReference type="PubMed" id="14631650"/>
            <dbReference type="DOI" id="10.1080/1042517031000149057"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17 24">Norris</strain>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="journal article" date="2020" name="Conserv Genet Resour" volume="12" first="433" last="446">
            <title>Fish mitochondrial genome sequencing: expanding genetic resources to support species detection and biodiversity monitoring using environmental DNA.</title>
            <authorList>
                <person name="Schroeter J.C."/>
                <person name="Maloy A.P."/>
                <person name="Rees C.B."/>
                <person name="Bartron M.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">NEFC_F16-049</strain>
            <strain evidence="20">NEFC_F16-259</strain>
            <strain evidence="22">NEFC_F16-262</strain>
            <strain evidence="18">NEFC_F16-263</strain>
            <strain evidence="21">NEFC_F16-568</strain>
        </source>
    </reference>
    <reference evidence="24" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <strain evidence="24">Norris</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Mitochondrion membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the complex I subunit 6 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.2" evidence="3 15"/>
    <dbReference type="EMBL" id="AF482987">
        <property type="protein sequence ID" value="AAL93233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="MF621716">
        <property type="protein sequence ID" value="ATU84714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="MF621718">
        <property type="protein sequence ID" value="ATU84740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="MF621720">
        <property type="protein sequence ID" value="ATU84766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="MF621721">
        <property type="protein sequence ID" value="ATU84779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="MF621722">
        <property type="protein sequence ID" value="ATU84792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_612136.1">
        <property type="nucleotide sequence ID" value="NC_003489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8SF45"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000013"/>
    <dbReference type="GeneID" id="804886"/>
    <dbReference type="KEGG" id="ipu:804886"/>
    <dbReference type="CTD" id="4541"/>
    <dbReference type="OMA" id="WVIYDTG"/>
    <dbReference type="OrthoDB" id="9837654at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Mitochondrion MT"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050269">
        <property type="entry name" value="ComplexI_Subunit6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001457">
        <property type="entry name" value="NADH_UbQ/plastoQ_OxRdtase_su6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11435">
        <property type="entry name" value="NADH UBIQUINONE OXIDOREDUCTASE SUBUNIT ND6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11435:SF1">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00499">
        <property type="entry name" value="Oxidored_q3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 15 17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11 15" id="KW-0520">NAD</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="8 15" id="KW-1278">Translocase</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 15" id="KW-0813">Transport</keyword>
    <keyword evidence="15" id="KW-0830">Ubiquinone</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013536034" description="NADH-ubiquinone oxidoreductase chain 6" evidence="16">
        <location>
            <begin position="16"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="133"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AAL93233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ATU84714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ATU84740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ATU84766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ATU84779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="ATU84792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="NP_612136.1"/>
        </source>
    </evidence>
    <sequence length="171" mass="18088" checksum="D6466F8D406F979E" modified="2002-06-01" version="1">MTYFLDLFLLSLVVGLVGVASNPAPYFAALGLAVAGGVGCGVLVGHGGSLVGLMLFLIYLGGMLVVFAYSAALAAEPFPETWGAGSVKVYVLMYLFGVGVAVWWFWGGHGGYWVIDEFKEFFMVRGDIGGISLMYSVGGGMLVVCAWVLLLCLFVILELTRGLSRGALRAV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2026-01-28" version="87" xmlns="https://uniprot.org/uniprot">
    <accession>Q9YGP3</accession>
    <name>GLHA_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName>Glycoprotein hormones alpha chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>GTH-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Gonadotropin alpha chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cga</name>
    </gene>
    <organism>
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="Mol. Mar. Biol. Biotechnol." volume="6" first="217" last="227">
            <title>Gonadotropin alpha-subunit glycoprotein from channel catfish (Ictalurus punctatus) and its expression during hormone-induced ovulation.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Li P."/>
                <person name="Argue B.J."/>
                <person name="Dunham R.A."/>
            </authorList>
            <dbReference type="PubMed" id="9284560"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [MRNA]</scope>
        <source>
            <strain>Kansas</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Shared alpha chain of heterodimeric glycoprotein hormones. These hormones bind specific receptors on target cells that in turn activate downstream signaling pathways. Involved in gametogenesis and steroidogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer. Glycoprotein hormones are heterodimers composed of a common alpha chain described here and a unique beta chain which confers their biological specificity to the different hormones.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycoprotein hormones subunit alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="AF112190">
        <property type="protein sequence ID" value="AAD18004.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187007.1">
        <property type="nucleotide sequence ID" value="NM_001200078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q9YGP3"/>
    <dbReference type="SMR" id="Q9YGP3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008246"/>
    <dbReference type="GlyCosmos" id="Q9YGP3">
        <property type="glycosylation" value="2 sites, No reported glycans"/>
    </dbReference>
    <dbReference type="GeneID" id="100304479"/>
    <dbReference type="KEGG" id="ipu:100304479"/>
    <dbReference type="CTD" id="1081"/>
    <dbReference type="OrthoDB" id="9852859at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016914">
        <property type="term" value="C:follicle-stimulating hormone complex"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016913">
        <property type="term" value="F:follicle-stimulating hormone activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0000353"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010893">
        <property type="term" value="P:positive regulation of steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010469">
        <property type="term" value="P:regulation of signaling receptor activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006590">
        <property type="term" value="P:thyroid hormone generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000011">
        <property type="entry name" value="Glycoprotein hormones alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000476">
        <property type="entry name" value="Glyco_hormone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11509">
        <property type="entry name" value="GLYCOPROTEIN HORMONE ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11509:SF0">
        <property type="entry name" value="GLYCOPROTEIN HORMONES ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00236">
        <property type="entry name" value="Hormone_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00274">
        <property type="entry name" value="GLYCOHORMONE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00067">
        <property type="entry name" value="GHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00779">
        <property type="entry name" value="GLYCO_HORMONE_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00780">
        <property type="entry name" value="GLYCO_HORMONE_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50277">
        <property type="entry name" value="GLYCO_HORMONE_ALPHA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-0372">Hormone</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0964">Secreted</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000011667" description="Glycoprotein hormones alpha chain">
        <location>
            <begin position="25"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="2">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="35"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="53"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="57"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P01215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="P37204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="116" mass="13089" checksum="C87498B2515B39BA" modified="1999-05-01" version="1" precursor="true">MILILKYTGATIILLSVLIEIGQLFPNNDFGCEECKLKENNIFSKPGAPVYQCMGCCFSRAYPTPLRSEKTMLVPKNITSEATCCVAKEVKRVIVNDVKLMNHTDCHCSTCYYHKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5U9V3</accession>
    <name>W5U9V3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12 14 15">Sororin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">cdca5-a</name>
        <name evidence="14 15" type="synonym">cdca5</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sororin family.</text>
    </comment>
    <dbReference type="EMBL" id="JT408419">
        <property type="protein sequence ID" value="AHH38339.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017314865.1">
        <property type="nucleotide sequence ID" value="XM_017459376.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017314866.1">
        <property type="nucleotide sequence ID" value="XM_017459377.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U9V3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021798"/>
    <dbReference type="GeneID" id="108259693"/>
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        <property type="component" value="Chromosome 28"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:mitotic sister chromatid cohesion"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Sororin"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31092:SF2">
        <property type="entry name" value="SORORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09666">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
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    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sororin-like middle region" evidence="10">
        <location>
            <begin position="88"/>
            <end position="224"/>
        </location>
    </feature>
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        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
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        </location>
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        <location>
            <begin position="1"/>
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            <begin position="94"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00093465"/>
        </source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF09666"/>
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        <source>
            <dbReference type="Pfam" id="PF25220"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="AHH38339.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017314865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017314866.1"/>
        </source>
    </evidence>
    <sequence length="262" mass="28651" checksum="5366E5489095E45D" modified="2014-04-16" version="1">MSSRTARAASIGQTSKNNNQSRKSDGGTPPRRRSARLSANEENMPPKAEASCVPVKRSITVRKIAPRKTQAASEPNKENVNKRVSGDSRKRSMVSSPTPVVETTVSKPMILSSIEVSASPPPHTGQPEEDPVWSRKVRRSYSRLSAGNLSFESPKPQSASSPDRRETLFGFERLQTPEVICKADVSRGAASSVCVRSFNLSAADDSIANTSEPDLNIPGVCMVKKNARRKRVHQIKMSELDLLAAKMNAEFEEAECFELVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
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    <name>W5UBN0_ICTPU</name>
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            <fullName evidence="12">Calcium homeostasis modulator protein 5 isoform X2</fullName>
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            <fullName evidence="10">Protein FAM26E</fullName>
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    </protein>
    <gene>
        <name evidence="10" type="primary">fam26e</name>
        <name evidence="12" type="synonym">LOC108280884</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CALHM family.</text>
    </comment>
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        <property type="molecule type" value="mRNA"/>
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        <property type="nucleotide sequence ID" value="XM_017496377.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UBN0"/>
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    <dbReference type="GeneID" id="108280884"/>
    <dbReference type="KEGG" id="ipu:108280884"/>
    <dbReference type="GeneTree" id="ENSGT01030000234610"/>
    <dbReference type="OMA" id="HYSTIHR"/>
    <dbReference type="OrthoDB" id="5953668at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904669">
        <property type="term" value="P:ATP export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029569">
        <property type="entry name" value="CALHM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32261">
        <property type="entry name" value="CALCIUM HOMEOSTASIS MODULATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32261:SF8">
        <property type="entry name" value="CALCIUM HOMEOSTASIS MODULATOR PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14798">
        <property type="entry name" value="Ca_hom_mod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
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            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AHH39255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017351866.1"/>
        </source>
    </evidence>
    <sequence length="316" mass="35960" checksum="A87228149DF57C0E" modified="2014-04-16" version="1">MIPASLKTTMDSLNAVVRFFTNQKTKIGYGIMAIATIGGERIFTLLSFQCPCNVNQNMVYGLTYLLGPALVLFTVGLFLSTRLWRLYTGCCLNPRKLCPRANCINCLSVLFHVILGALVAPIMWLSIALLNGTFYECAVSGLEAQALVHWFCKNRTSTCRDQLAKVPCQTSNMSAADTKELLLLLHAQSQILGWVLIMSTAVAALVGTCYKNCRSQVSYLQLTFWKVYKEKENEKFDSFADEYSNRLAERNLKSFFENKDPEVFPFPNHKAWEQISTLYTYNRGEQYYSTLQRYVEDPNRDYTPENMPLEMEHSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5UBW0</accession>
    <name>W5UBW0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13 15">Sushi domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Susd2</name>
        <name evidence="15" type="synonym">susd2</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="protein sequence ID" value="AHH39506.1"/>
        <property type="molecule type" value="mRNA"/>
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        <property type="nucleotide sequence ID" value="XM_017451936.3"/>
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    <dbReference type="KEGG" id="ipu:108255750"/>
    <dbReference type="CTD" id="56241"/>
    <dbReference type="GeneTree" id="ENSGT00730000110943"/>
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        <property type="component" value="Chromosome 22"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="SUSHI DOMAIN-CONTAINING PROTEIN 2"/>
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            <end position="828"/>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50958"/>
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        <source>
            <dbReference type="PROSITE" id="PS51233"/>
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        <source>
            <dbReference type="EMBL" id="AHH39506.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017307425.1"/>
        </source>
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</entry>
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    <accession>W5UDK9</accession>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
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                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
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    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="5">Blood</tissue>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
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        <property type="entry name" value="CLATHRIN INTERACTOR 1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="436"/>
            <end position="461"/>
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        <location>
            <begin position="219"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="297"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="436"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AHH37752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017329500.1"/>
        </source>
    </evidence>
    <sequence length="612" mass="66910" checksum="FCBA17DE7AFCEC85" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5UFZ4</accession>
    <name>W5UFZ4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutathione S-transferase C-terminal domain-containing protein</fullName>
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    </protein>
    <gene>
        <name evidence="7 9" type="primary">gstcd</name>
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    <organism evidence="7">
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
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            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GSTCD family.</text>
    </comment>
    <dbReference type="EMBL" id="JT414078">
        <property type="protein sequence ID" value="AHH40489.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017318795.1">
        <property type="nucleotide sequence ID" value="XM_017463306.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UFZ4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012896"/>
    <dbReference type="GeneID" id="108262955"/>
    <dbReference type="KEGG" id="ipu:108262955"/>
    <dbReference type="CTD" id="79807"/>
    <dbReference type="OrthoDB" id="206598at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000125">
        <property type="entry name" value="Glutathione S-transferase C-terminal domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13369">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13369:SF0">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13679">
        <property type="entry name" value="Methyltransf_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="6">
        <location>
            <begin position="418"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="197"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="346"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="199"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AHH40489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017318795.1"/>
        </source>
    </evidence>
    <sequence length="628" mass="69563" checksum="0F7038DFC26C3651" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5UHC0</accession>
    <name>W5UHC0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">sphingomyelin phosphodiesterase</fullName>
            <ecNumber evidence="4">3.1.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Smpd3</name>
        <name evidence="14 15" type="synonym">smpd5</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N-(hexadecanoyl)-sphing-4-enine-1-phosphocholine + H2O = N-hexadecanoylsphing-4-enine + phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45644"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72959"/>
            <dbReference type="ChEBI" id="CHEBI:78646"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:45645"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a sphingomyelin + H2O = phosphocholine + an N-acylsphing-4-enine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19253"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17636"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
            <dbReference type="EC" id="3.1.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:19254"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Sphingolipid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the neutral sphingomyelinase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.12" evidence="4"/>
    <dbReference type="EMBL" id="JT415282">
        <property type="protein sequence ID" value="AHH40974.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017308404.1">
        <property type="nucleotide sequence ID" value="XM_017452915.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017308406.1">
        <property type="nucleotide sequence ID" value="XM_017452917.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UHC0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000011269"/>
    <dbReference type="GeneID" id="108256263"/>
    <dbReference type="KEGG" id="ipu:108256263"/>
    <dbReference type="CTD" id="392275"/>
    <dbReference type="OMA" id="LGCWAPA"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004767">
        <property type="term" value="F:sphingomyelin phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006684">
        <property type="term" value="P:sphingomyelin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09078">
        <property type="entry name" value="nSMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000114">
        <property type="entry name" value="Sphingomyelin phosphodiesterase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038772">
        <property type="entry name" value="Sph/SMPD2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017766">
        <property type="entry name" value="Sphingomyelinase/PLipase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320:SF9">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16320">
        <property type="entry name" value="SPHINGOMYELINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="53"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="11">
        <location>
            <begin position="263"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AHH40974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017308404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017308406.1"/>
        </source>
    </evidence>
    <sequence length="564" mass="62478" checksum="08BCEC0BA60289C8" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5UJ78</accession>
    <name>W5UJ78_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Forkhead box A sequence isoform X1</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="7">Forkhead box protein A2-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">foxa2-a</name>
        <name evidence="9" type="synonym">foxa</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT412082">
        <property type="protein sequence ID" value="AHH39722.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017329003.1">
        <property type="nucleotide sequence ID" value="XM_017473514.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UJ78"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027757"/>
    <dbReference type="GeneID" id="108268493"/>
    <dbReference type="KEGG" id="ipu:108268493"/>
    <dbReference type="CTD" id="30539"/>
    <dbReference type="GeneTree" id="ENSGT00940000155999"/>
    <dbReference type="OrthoDB" id="5954824at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000042">
        <property type="entry name" value="hepatocyte nuclear factor 3-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF380">
        <property type="entry name" value="PROTEIN FORK HEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fork-head" evidence="6">
        <location>
            <begin position="97"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="4">
        <location>
            <begin position="97"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="192"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="258"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="195"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="208"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="226"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AHH39722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017329003.1"/>
        </source>
    </evidence>
    <sequence length="381" mass="43319" checksum="5E3618E3B7CE016B" modified="2014-04-16" version="1">MIGGVKQERCDEWPSFYTNQTYRGADSMRLSSGPYSSPEHPYHVFSLQRESLAGEDIEKIETPPDVLVQQRCPEADHNRLELPDLKSVYRRSFNHTKPPYSYISLIYMAIQQSPSKKLTLNEIYDWIRQLFPYYRQNQQRWQNSIRHSLSFNDCFVRVPRSPDSPGKGSFWTLHPDSGNMFENGCYMRRQKRFRCQSTSPSTTKNASKKADRETVKEEGKKKTSEVKSTVLLSISQPQPSKPPMPTMFPTVMDCPPMSPSHIPPQQHTTHSLTPFPPSSESSAHLTNIPCPTMSTLQPTTSCSLEPCVHGEPFFHQSLSVPPIMDFQCYEPPVSYPVYYPSSSSSIHQYNPYLTGKEESSYAGDSVCYSGLMCPLPLLSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5UJC9</accession>
    <name>W5UJC9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10 12">Mannose-1-phosphate guanyltransferase alpha-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 12" type="primary">gmppab</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Regulatory subunit of the GMPPA-GMPPB mannose-1-phosphate guanylyltransferase complex; reduces the catalytic activity of GMPPB when part of the complex. Mediates allosteric feedback inhibition of GMPPB catalytic activity upon binding GDP-alpha-D-mannose. Together with GMPPB regulates GDP-alpha-D-mannose levels. One of two paralogs (gmppaa and gmppab) that may have redundant functions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EMBL" id="JT416708">
        <property type="protein sequence ID" value="AHH42121.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017338031.1">
        <property type="nucleotide sequence ID" value="XM_017482542.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UJC9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019646"/>
    <dbReference type="GeneID" id="108273364"/>
    <dbReference type="KEGG" id="ipu:108273364"/>
    <dbReference type="CTD" id="393469"/>
    <dbReference type="OrthoDB" id="285674at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06428">
        <property type="entry name" value="M1P_guanylylT_A_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000071">
        <property type="entry name" value="Mannose-1-phosphate guanyltransferase alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="8">
        <location>
            <begin position="3"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="9">
        <location>
            <begin position="285"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AHH42121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017338031.1"/>
        </source>
    </evidence>
    <sequence length="422" mass="47312" checksum="40EFBDB756E5BCE3" modified="2014-04-16" version="1">MLKAVILIGGPQKGTRFRPLSFEVPKPLFPVAGVPMLQHHIEACAKVPNMKEIILIGFYQPNEELNRFIASAQQEFKFSVRYLQEFAALGTGGGIYHFRDQILCGGPSAFFLMNADVCSEFPLQDMLHFHHQHGNSHCGIILGTTANRKQSLNYGCMVENQETNEVLHFVEKPSTFVSDIINCGIYLFTPKIFTHIGTVFQRKQEDRQLEEPPCARQLRELIRLEQDIFTMLAAQKQLYVYKTQHFWSQIKSAGSAIYASRLYLNRYNETHPERLARYQEGGPKIIGNVYIHQTANIDSTAVLGPNVSIGKGVIIGAGVRVRESIVLHGATLQDHCCVLNSIVGWDSTIGRWARVEGTPSDPNPNDPFAKIDSETLFRDGGLTPSITILGCNVNIPSEVLIRNSIVLPHKDLNRSFKNQIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5UKX6</accession>
    <name>W5UKX6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phosphatidylinositol transfer protein alpha isoform</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">PITPNA</name>
        <name evidence="18" type="synonym">pitpnaa</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the transfer of phosphatidylinositol (PI) and phosphatidylcholine (PC) between membranes. Shows a preference for PI and PC containing shorter saturated or monosaturated acyl chains at the sn-1 and sn-2 positions. Preference order for PC is C16:1 &gt; C16:0 &gt; C18:1 &gt; C18:0 &gt; C20:4 and for PI is C16:1 &gt; C16:0 &gt; C18:1 &gt; C18:0 &gt; C20:4 &gt; C20:3.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(out)</text>
            <dbReference type="Rhea" id="RHEA:38691"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:38692"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phosphocholine(out)</text>
            <dbReference type="Rhea" id="RHEA:38571"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:38572"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the PtdIns transfer protein family. PI transfer class I subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="JT413558">
        <property type="protein sequence ID" value="AHH40302.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017346131.1">
        <property type="nucleotide sequence ID" value="XM_017490642.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UKX6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034466"/>
    <dbReference type="GeneID" id="108277750"/>
    <dbReference type="KEGG" id="ipu:108277750"/>
    <dbReference type="CTD" id="393909"/>
    <dbReference type="GeneTree" id="ENSGT00940000157119"/>
    <dbReference type="OMA" id="NFTEWIS"/>
    <dbReference type="OrthoDB" id="18453at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008525">
        <property type="term" value="F:phosphatidylcholine transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043009">
        <property type="term" value="P:chordate embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd08888">
        <property type="entry name" value="SRPBCC_PITPNA-B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000004">
        <property type="entry name" value="Phosphatidylinositol transfer protein alpha isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001666">
        <property type="entry name" value="PI_transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055261">
        <property type="entry name" value="PI_transfer_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10658">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10658:SF28">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN ALPHA ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02121">
        <property type="entry name" value="IP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00391">
        <property type="entry name" value="PITRANSFER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Phosphatidylinositol transfer protein N-terminal" evidence="15">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="150"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="250"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AHH40302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017346131.1"/>
        </source>
    </evidence>
    <sequence length="270" mass="31695" checksum="2E7D37D67E1F846E" modified="2014-04-16" version="1">MLIKEYRVVLPISVEEYQVAQLYSVAEASKNETGGGEGVEVLKNEPYEKDGEKGQYTHKIYHLQSKVPGFVRMLAPASALKVHEKAWNAYPYCRTILTNEYMKDNFMIMIETWHKPDYGDQENVHKLDAEAWKKTEVVYIDIADRSQVDDKDYKPEEDPATFKSTKTGRGPLGPQWRKELPQKTDCPHMCAYKLVTVKFKWFGLQNKVEGFIHKQEKRLFTNFHRQLFCWIDRWIDLTMDDIRRMEAETQKELDKMREKDPVKGMSASDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5ULP7</accession>
    <name>W5ULP7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Atypical kinase COQ8A, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Chaperone activity of bc1 complex-like</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Coenzyme Q protein 8A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">aarF domain-containing protein kinase 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">adck3</name>
        <name evidence="23 24" type="synonym">coq8ab</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23 24" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23 24">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Atypical kinase involved in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration. Its substrate specificity is still unclear: may act as a protein kinase that mediates phosphorylation of COQ3. According to other reports, acts as a small molecule kinase, possibly a lipid kinase that phosphorylates a prenyl lipid in the ubiquinone biosynthesis pathway, as suggested by its ability to bind coenzyme Q lipid intermediates. However, the small molecule kinase activity was not confirmed by another publication. Shows an unusual selectivity for binding ADP over ATP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="JT418830">
        <property type="protein sequence ID" value="AHH42856.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017331776.1">
        <property type="nucleotide sequence ID" value="XM_017476287.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017331777.1">
        <property type="nucleotide sequence ID" value="XM_017476288.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ULP7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005257"/>
    <dbReference type="GeneID" id="108270043"/>
    <dbReference type="KEGG" id="ipu:108270043"/>
    <dbReference type="CTD" id="327332"/>
    <dbReference type="GeneTree" id="ENSGT00940000156810"/>
    <dbReference type="OMA" id="MSRELAW"/>
    <dbReference type="OrthoDB" id="201153at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13970">
        <property type="entry name" value="ABC1_ADCK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034646">
        <property type="entry name" value="ADCK3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051409">
        <property type="entry name" value="Atypical_kinase_ADCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851:SF1">
        <property type="entry name" value="ATYPICAL KINASE COQ8A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 23 24" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="20">
        <location>
            <begin position="254"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00033204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00058956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="AHH42856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017331776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_017331777.1"/>
        </source>
    </evidence>
    <sequence length="604" mass="68417" checksum="925CC22B583A97BF" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5UQX9</accession>
    <name>W5UQX9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Acetyl-coenzyme A synthetase</fullName>
            <ecNumber evidence="9">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ACSS2</name>
        <name evidence="15" type="synonym">acss2l</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + CoA = acetyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23176"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:23177"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>propanoate + ATP + CoA = propanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20373"/>
            <dbReference type="ChEBI" id="CHEBI:17272"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.17"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:20374"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer. Interacts with TFEB. AMPK-mediated phosphorylated form at Ser-659 interacts with KPNA1; this interaction results in nuclear translocation of ACSS2. Interacts with the 'Thr-172' phosphorylated form of PRKAA2. Interacts with CREBBP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="9"/>
    <dbReference type="EMBL" id="JT416219">
        <property type="protein sequence ID" value="AHH41978.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017351336.1">
        <property type="nucleotide sequence ID" value="XM_017495847.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UQX9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000025922"/>
    <dbReference type="GeneID" id="108280639"/>
    <dbReference type="KEGG" id="ipu:108280639"/>
    <dbReference type="CTD" id="560020"/>
    <dbReference type="OMA" id="ETPLFML"/>
    <dbReference type="OrthoDB" id="1706066at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050218">
        <property type="term" value="F:propionate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019427">
        <property type="term" value="P:acetyl-CoA biosynthetic process from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05966">
        <property type="entry name" value="ACS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000004">
        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000001">
        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011904">
        <property type="entry name" value="Ac_CoA_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02188">
        <property type="entry name" value="Ac_CoA_lig_AcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001208">
        <property type="entry name" value="PRK00174.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF146">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="12">
        <location>
            <begin position="32"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="10">
        <location>
            <begin position="103"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="11">
        <location>
            <begin position="556"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AHH41978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017351336.1"/>
        </source>
    </evidence>
    <sequence length="674" mass="76561" checksum="A295E5FF5318493A" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PK00</accession>
    <name>A0A2D0PK00_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">CMP-sialic acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">slc35a1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleotide-sugar transporter family. SLC35A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017306342.1">
        <property type="nucleotide sequence ID" value="XM_017450853.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PK00"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022890"/>
    <dbReference type="GeneID" id="108255137"/>
    <dbReference type="KEGG" id="ipu:108255137"/>
    <dbReference type="CTD" id="10559"/>
    <dbReference type="OMA" id="KCYVIAS"/>
    <dbReference type="OrthoDB" id="408493at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015165">
        <property type="term" value="F:pyrimidine nucleotide-sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007271">
        <property type="entry name" value="Nuc_sug_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00803">
        <property type="entry name" value="nst"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10231">
        <property type="entry name" value="NUCLEOTIDE-SUGAR TRANSMEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04142">
        <property type="entry name" value="Nuc_sug_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005799">
        <property type="entry name" value="UDP-gal_transpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="271"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="294"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017306342.1"/>
        </source>
    </evidence>
    <sequence length="337" mass="36760" checksum="5C4731EF43C8A434" modified="2017-12-20" version="1">MAAGSVSVVFKLYCLVVMTLVAAVYTVALRYTRTVQKELYFSTTAVCLAEIIKLFLSLGMLTRETGGLNRCKGVVVEQVFNPVDLLKLSVPSVVYAVQNNMAFLALSNLDAAVYQVTYQLKIPCTALCTVLMLNRSLSRIQWFSVFMLCLGVILVQWKPAELTKVQVEQNPVVGFGAIAIAVLCSGFAGVYFEKVLKSSETSLWVRNIQMYLSGICITLLGVFMNNGAQVMEKGFFFGYTPWVCFVVFLASVGGLYTSVVVKYTDNIMKGFSAAAAIVLSTIASVTLFGLQITVTFSTGALLVCVSIYMYGLPKQDTSTLTKASEDDKASREKLVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PLR8</accession>
    <name>A0A2D0PLR8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MOB kinase activator 1A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mob1a</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017307543.1">
        <property type="nucleotide sequence ID" value="XM_017452054.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PLR8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030776"/>
    <dbReference type="GeneID" id="108255806"/>
    <dbReference type="KEGG" id="ipu:108255806"/>
    <dbReference type="CTD" id="55233"/>
    <dbReference type="OMA" id="HKHAKAT"/>
    <dbReference type="OrthoDB" id="8170117at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.30:FF:000001">
        <property type="entry name" value="MOB kinase activator 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.30">
        <property type="entry name" value="MOB kinase activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005301">
        <property type="entry name" value="MOB_kinase_act_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036703">
        <property type="entry name" value="MOB_kinase_act_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22599">
        <property type="entry name" value="MPS ONE BINDER KINASE ACTIVATOR-LIKE MOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03637">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01388">
        <property type="entry name" value="Mob1_phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101152">
        <property type="entry name" value="Mob1/phocein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR605301-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017307543.1"/>
        </source>
    </evidence>
    <sequence length="216" mass="25064" checksum="6F22A40BA6D4E34B" modified="2017-12-20" version="1">MSFLFGSRSSKTFKPKKNIPEGSHQYELLKHAEATLGSGNLRAAVMLPEGEDLNEWIAVNTVDFFNQINMLYGTITEFCTETNCPVMSAGPKYEYHWADGTNIKKPIKCSAPKYIDYLMTWVQDQLDDETLFPSKIGVPFPKNFMSVAKTILKRLFRVYAHIYHQHFDSVMQLQEEAHLNTSFKHFIFFVQEFNLIDRRELVPLQELIEKLGTKDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PMB2</accession>
    <name>A0A2D0PMB2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BOLA class I histocompatibility antigen, alpha chain BL3-7 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC108255578</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the MHC class I family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017307139.1">
        <property type="nucleotide sequence ID" value="XM_017451650.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PMB2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034517"/>
    <dbReference type="GeneID" id="108255578"/>
    <dbReference type="KEGG" id="ipu:108255578"/>
    <dbReference type="OMA" id="WDGEKER"/>
    <dbReference type="OrthoDB" id="8846792at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07698">
        <property type="entry name" value="IgC1_MHC_I_alpha3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.500.10:FF:000001">
        <property type="entry name" value="H-2 class I histocompatibility antigen, alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001039">
        <property type="entry name" value="MHC_I_a_a1/a2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF237">
        <property type="entry name" value="MHC CLASS I ANTIGEN TRANSCRIPT VARIANT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00129">
        <property type="entry name" value="MHC_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01638">
        <property type="entry name" value="MHCCLASSI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5011977016" evidence="4">
        <location>
            <begin position="18"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="189"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017307139.1"/>
        </source>
    </evidence>
    <sequence length="341" mass="38627" checksum="B9E41AD25B3862B8" modified="2017-12-20" version="1">MMEILFLFTACFLHVSADTHSLQHLYTAVTSKIHFPSVEFTAVDLLNGEQITSYNGNNQTLIPKDWIKNITEETYWKSKTQDMQGNQETFTRKFTTVMESFNHSNEGHTLQRMYGCERDDDNTIRGYDVYGYDGEDFISLDLKTGTWTAASAKAVNIKHKWKSKEAKYQKNYLENECIEWLAKDRKVCPEASVFQKNSSSPEVVCHATGFFPKTVMITWQKDGEDVHEGVDLRETLPNQDGSFQKRSILTVSAEDLQKHTYTCVIQHSSLETDIIMPVRERPILRGRRLSRGRIGVIAAVAVVFVAIVAGIVVWKRYSSGFRNVPQNISSSGGALSSNNSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PRL4</accession>
    <name>A0A2D0PRL4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Epithelial splicing regulatory protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">esrp1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of fgfr2. Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of fgfr2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESRP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017308664.1">
        <property type="nucleotide sequence ID" value="XM_017453175.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PRL4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027536"/>
    <dbReference type="GeneID" id="108256377"/>
    <dbReference type="KEGG" id="ipu:108256377"/>
    <dbReference type="CTD" id="54845"/>
    <dbReference type="OMA" id="FTLTFHV"/>
    <dbReference type="OrthoDB" id="431068at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904888">
        <property type="term" value="P:cranial skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12742">
        <property type="entry name" value="RRM3_ESRP1_ESRP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000041">
        <property type="entry name" value="Epithelial splicing regulatory protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000070">
        <property type="entry name" value="Epithelial splicing regulatory protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000056">
        <property type="entry name" value="epithelial splicing regulatory protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050666">
        <property type="entry name" value="ESRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13976">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="224"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="325"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017308664.1"/>
        </source>
    </evidence>
    <sequence length="716" mass="78317" checksum="E9840B358ABAD372" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PTM4</accession>
    <name>A0A2D0PTM4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein asteroid homolog 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">aste1a</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <tissue evidence="4">Blood</tissue>
        </source>
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        <property type="entry name" value="PIN domain-like"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <begin position="133"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF12813"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017308445.1"/>
        </source>
    </evidence>
    <sequence length="649" mass="74019" checksum="646E67F7AEC10F0D" modified="2017-12-20" version="1"> </sequence>
</entry>
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            <fullName evidence="14">Olfactory receptor</fullName>
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        <name evidence="17" type="primary">LOC108256845</name>
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        <name type="scientific">Ictalurus punctatus</name>
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    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
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                <person name="Duke M.V."/>
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                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
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                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
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    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017309557.1">
        <property type="nucleotide sequence ID" value="XM_017454068.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PU70"/>
    <dbReference type="GeneID" id="108256845"/>
    <dbReference type="KEGG" id="ipu:108256845"/>
    <dbReference type="OrthoDB" id="10017003at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:olfactory receptor activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="22"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="236"/>
            <end position="255"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017309557.1"/>
        </source>
    </evidence>
    <sequence length="311" mass="36315" checksum="6B14E6F36BA7EDBC" modified="2017-12-20" version="1">MDYYNVTYLTLEGQVELEKYRYVYFVMVLTLYLLIISCNGLVIFVIFTNKCLHEPMYIFIAALLCNALYGSTALYPKLLIDFLSEIQIVSYEACIFQSFFIYTYAISEFTLLSAMAYDRYVSICKPLQYATIIKMSTVKRLLFCCWCLPCCENSIALTLTFQVKLCRFKLNRIYCNNSSVVKLSCGDISVRNSYALFIFVIAVFPPVIFVIYSYIRILHVCLRSSNDFRRKALQTCFPHIFIFISYTVTSCFEVINNRLESNQIPHIFTMIMSVENLVIPPLLNPIIYGLKMQKIFNCIKKLIWKVKQMLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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    </protein>
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    <organism evidence="7 8">
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            <taxon>Neopterygii</taxon>
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        </lineage>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM83 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017454287.2"/>
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        <property type="entry name" value="Endonuclease Chain A"/>
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    <sequence length="1232" mass="139328" checksum="1024586F3C79EC4B" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PXD5</accession>
    <name>A0A2D0PXD5_ICTPU</name>
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        <recommendedName>
            <fullName evidence="10">Phosphodiesterase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">pde4a</name>
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    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="13">SDA103</strain>
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    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
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        <reaction evidence="6">
            <text>3',5'-cyclic AMP + H2O = AMP + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
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            <dbReference type="ChEBI" id="CHEBI:456215"/>
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        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:25278"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.</text>
    </comment>
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        <property type="reaction ID" value="UER00747"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
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        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0114">cAMP</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="12">
        <location>
            <begin position="326"/>
            <end position="655"/>
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    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
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            <begin position="753"/>
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    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="402"/>
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    <feature type="binding site" evidence="8">
        <location>
            <begin position="402"/>
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        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
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    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="406"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
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    </feature>
    <feature type="binding site" evidence="9">
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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        <ligand>
            <name>AMP</name>
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            <name>Zn(2+)</name>
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            <name>Zn(2+)</name>
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        <ligand>
            <name>AMP</name>
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    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017310699.1"/>
        </source>
    </evidence>
    <sequence length="771" mass="86898" checksum="2C7EED09E213D22F" modified="2017-12-20" version="1"> </sequence>
</entry>
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    <accession>A0A2D0PXQ6</accession>
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        <submittedName>
            <fullName evidence="5">Uncharacterized protein LOC108257189 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC108257189</name>
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    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017310211.1">
        <property type="nucleotide sequence ID" value="XM_017454722.2"/>
    </dbReference>
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    <dbReference type="GeneID" id="108257189"/>
    <dbReference type="KEGG" id="ipu:108257189"/>
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        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="APPLE_Factor_XI_like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000177">
        <property type="entry name" value="Apple"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33946:SF4">
        <property type="entry name" value="COAGULATION FACTOR XI"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="8"/>
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    </dbReference>
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            <end position="316"/>
        </location>
    </feature>
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        <location>
            <begin position="415"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="3">
        <location>
            <begin position="595"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017310211.1"/>
        </source>
    </evidence>
    <sequence length="773" mass="89713" checksum="5B6E9EC245CA6844" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PZA0</accession>
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        <submittedName>
            <fullName evidence="7 8">Kinase D-interacting substrate of 220 kDa B isoform X1</fullName>
        </submittedName>
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        <name evidence="7 8" type="primary">kidins220b</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7 8">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017311813.1">
        <property type="nucleotide sequence ID" value="XM_017456324.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017311814.1">
        <property type="nucleotide sequence ID" value="XM_017456325.3"/>
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    <dbReference type="KEGG" id="ipu:108258057"/>
    <dbReference type="CTD" id="335881"/>
    <dbReference type="GeneTree" id="ENSGT00940000156714"/>
    <dbReference type="OrthoDB" id="6084525at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0030165">
        <property type="term" value="F:PDZ domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
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    <dbReference type="InterPro" id="IPR011646">
        <property type="entry name" value="KAP_P-loop"/>
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    <dbReference type="InterPro" id="IPR057092">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24116:SF2">
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        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07693">
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    </dbReference>
    <dbReference type="Pfam" id="PF23307">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="10"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="506"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="533"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="666"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
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            <end position="721"/>
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    </feature>
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        <location>
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    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
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            <end position="110"/>
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    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="111"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
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        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
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    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
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    </feature>
    <feature type="repeat" description="ANK" evidence="1">
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    </feature>
    <feature type="repeat" description="ANK" evidence="1">
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    </feature>
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    </feature>
    <feature type="repeat" description="ANK" evidence="1">
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    <feature type="region of interest" description="Disordered" evidence="2">
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    <feature type="region of interest" description="Disordered" evidence="2">
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
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            <begin position="1529"/>
            <end position="1541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1548"/>
            <end position="1560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1561"/>
            <end position="1574"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1632"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1661"/>
            <end position="1679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1684"/>
            <end position="1698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017311813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017311814.1"/>
        </source>
    </evidence>
    <sequence length="1738" mass="192750" checksum="FB16EA70E201FB80" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q4U2</accession>
    <name>A0A2D0Q4U2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Carboxypeptidase O</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">cpo</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Carboxypeptidase which preferentially cleaves C-terminal acidic residues from peptides and proteins. Can also cleave C-terminal hydrophobic amino acids, with a preference for small residues over large residues.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017312380.1">
        <property type="nucleotide sequence ID" value="XM_017456891.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q4U2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006974"/>
    <dbReference type="GeneID" id="108258336"/>
    <dbReference type="KEGG" id="ipu:108258336"/>
    <dbReference type="CTD" id="130749"/>
    <dbReference type="OMA" id="EEQWAGQ"/>
    <dbReference type="OrthoDB" id="3626597at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000050">
        <property type="entry name" value="Carboxypeptidase O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF19">
        <property type="entry name" value="CARBOXYPEPTIDASE O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 22" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 19" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012135470" description="Carboxypeptidase O" evidence="19">
        <location>
            <begin position="24"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="20">
        <location>
            <begin position="33"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="18">
        <location>
            <position position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00058352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00071404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017312380.1"/>
        </source>
    </evidence>
    <sequence length="362" mass="40924" checksum="5D8DECC436971D06" modified="2017-12-20" version="1">MLGSVFSVLLVLTLQTHDSSVEGLEQRAYDYTKYHTMDEIMQWMWTMEKDHPDVVTSVIYGTTYEKRNITLLKIGLNSTKQKKIIWMDCGIHAREWIAPAFCQHFVKEILGSYKTDSKISEMLKNLDFYITPVLNMDGYLYSWKDNTTRLWRKTRSPGSENCTCFGTDLNRNFYANWGMVGISRNCCSEIYNGKSPLSEPEAQAVTDFLSNNRDNILCFLTIHSYGQLILLPYGHPNITAPNQDELMKVGLAAAEAIKAVHSMSYTVGTPPDVLYPNSGSSRDFARLIGIPFSFTFELRDKGDHGFKLPEDQIQPTCEEAYQGALSIITYVHDKSYVRAAATSIAATLWSVVMAVWLSSATD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q4Z8</accession>
    <name>A0A2D0Q4Z8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Nicotinate phosphoribosyltransferase</fullName>
            <ecNumber evidence="3 11">6.3.4.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">naprt</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the first step in the biosynthesis of NAD from nicotinic acid, the ATP-dependent synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate. Helps prevent cellular oxidative stress via its role in NAD biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + nicotinate + ATP + H2O = nicotinate beta-D-ribonucleotide + ADP + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.</text>
    </comment>
    <comment type="PTM">
        <text evidence="11">Transiently phosphorylated on a His residue during the reaction cycle. Phosphorylation strongly increases the affinity for substrates and increases the rate of nicotinate D-ribonucleotide production. Dephosphorylation regenerates the low-affinity form of the enzyme, leading to product release.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the NAPRTase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.21" evidence="3 11"/>
    <dbReference type="RefSeq" id="XP_017313374.1">
        <property type="nucleotide sequence ID" value="XM_017457885.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q4Z8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005772"/>
    <dbReference type="GeneID" id="108258894"/>
    <dbReference type="KEGG" id="ipu:108258894"/>
    <dbReference type="CTD" id="93100"/>
    <dbReference type="OMA" id="VYFPGSP"/>
    <dbReference type="OrthoDB" id="193380at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00457"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004516">
        <property type="term" value="F:nicotinate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034355">
        <property type="term" value="P:NAD+ biosynthetic process via the salvage pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01570">
        <property type="entry name" value="NAPRTase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.140.10:FF:000012">
        <property type="entry name" value="Nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.140.10">
        <property type="entry name" value="nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
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        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
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        <property type="entry name" value="NAPRTase_put"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NICOTINATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11098:SF1">
        <property type="entry name" value="NICOTINATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NAPRTase_C"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NAPRTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAPRT"/>
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    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
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    </dbReference>
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        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        <location>
            <begin position="23"/>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017313374.1"/>
        </source>
    </evidence>
    <sequence length="544" mass="59512" checksum="2C16A675B82D4DB7" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q5W6</accession>
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        <submittedName>
            <fullName evidence="18">Adhesion G protein-coupled receptor E3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC108258741</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
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    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.</text>
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        <property type="nucleotide sequence ID" value="XM_017457607.3"/>
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    <dbReference type="GeneID" id="108258741"/>
    <dbReference type="KEGG" id="ipu:108258741"/>
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        <property type="component" value="Chromosome 26"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:cell surface receptor signaling pathway"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Adhesion G protein-coupled receptor E3"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GAIN_B"/>
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        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="GPCR_2-like_7TM"/>
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        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
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        <property type="entry name" value="GPS"/>
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        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
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        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
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    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
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    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012836002" evidence="14">
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        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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            <end position="601"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="621"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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        </location>
    </feature>
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        <location>
            <begin position="267"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="16">
        <location>
            <begin position="426"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017313096.1"/>
        </source>
    </evidence>
    <sequence length="707" mass="78077" checksum="460389FC91D0B108" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q9H8</accession>
    <name>A0A2D0Q9H8_ICTPU</name>
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        <submittedName>
            <fullName evidence="6">Uncharacterized protein LOC108259741 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC108259741</name>
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    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017314949.1">
        <property type="nucleotide sequence ID" value="XM_017459460.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q9H8"/>
    <dbReference type="GeneID" id="108259741"/>
    <dbReference type="KEGG" id="ipu:108259741"/>
    <dbReference type="OMA" id="WDHESIG"/>
    <dbReference type="OrthoDB" id="9928644at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032190">
        <property type="term" value="F:acrosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035803">
        <property type="term" value="P:egg coat formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000344">
        <property type="term" value="P:positive regulation of acrosome reaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.4100:FF:000002">
        <property type="entry name" value="Zona pellucida sperm-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055356">
        <property type="entry name" value="ZP-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576:SF3">
        <property type="entry name" value="SI:CH211-14A17.6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23344">
        <property type="entry name" value="ZP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012903682" evidence="3">
        <location>
            <begin position="21"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="326"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="4">
        <location>
            <begin position="25"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017314949.1"/>
        </source>
    </evidence>
    <sequence length="370" mass="41535" checksum="9E80C7A3ED9A8E5F" modified="2017-12-20" version="1">MKMILMQGILVLLFVVSTFEQKVTDCSANGVTIQWRPELSQVQKLDPSKARLGSCPPSSMSMEDDTLLFSVGFHDCGFRKLVSEDKVMYTNLLTYNLDAGLPPITELVACVYDLSSNSTDLDNVRNDLVFTLELMNSDFNGPAPSTMFTFGTRIPIRAKVEQQVLGPLWIYLQYCMLANTQDLTQASRLHTLISDDGCLIESKEGNATFLPRREPSEILLYFQAFKFASGEQVFLHCDMTTSDLQSFSVDKKACQYQNGWGWELLDDPTRSYICNCCDSTCIQRTGFESGVYTRQVLGPFTMVDSQHSAEDESTFRSDEKGLGVPVWVVGLSVCLVLMLIAGSIASSYYLCFWRGGRLGYRPSRELLNKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QEA3</accession>
    <name>A0A2D0QEA3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Hairy and enhancer of split-related protein HELT</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">HES/HEY-like transcription factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">helt</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the HEY family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017316637.1">
        <property type="nucleotide sequence ID" value="XM_017461148.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QEA3"/>
    <dbReference type="GeneID" id="108260700"/>
    <dbReference type="KEGG" id="ipu:108260700"/>
    <dbReference type="CTD" id="391723"/>
    <dbReference type="OMA" id="FGYHECM"/>
    <dbReference type="OrthoDB" id="6371181at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000054">
        <property type="entry name" value="hairy and enhancer of split-related protein HELT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07527">
        <property type="entry name" value="Hairy_orange"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158457">
        <property type="entry name" value="Orange domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="11">
        <location>
            <begin position="73"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="12">
        <location>
            <begin position="150"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017316637.1"/>
        </source>
    </evidence>
    <sequence length="276" mass="32069" checksum="0BDFBE217CC50DF2" modified="2017-12-20" version="1">MRTIRFIKRPDSRHTHTHTHTYTPTHTPQSQSHSLTQEPTVLCTATFLDLWQQLLFICISKEMMASKMKDRKRTPVSHKVIEKRRRDRINRCLNELGKTVPMALAKQNCGKLEKAEILEMTVQYLRALHSADFPRGREKGELLSEFANYFHYGYHECMKNLVHYLTTVERTETKDNKYARILAFLQSKVVSEPVFGSRYESAFTSPEPVDFLCQLRSPPECHQNPSEPVFQQSHGGHLSWHGSALAYPQQHGLDHHHYMNFMSHTHSLHTTPHVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QJW9</accession>
    <name>A0A2D0QJW9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Histone H2B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC108261899</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="12">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017318141.2">
        <property type="nucleotide sequence ID" value="XM_017462652.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QJW9"/>
    <dbReference type="GeneID" id="108261899"/>
    <dbReference type="KEGG" id="ipu:108261899"/>
    <dbReference type="OrthoDB" id="10036053at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000003">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 12" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11 12" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="14">
        <location>
            <begin position="23"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017318141.2"/>
        </source>
    </evidence>
    <sequence length="124" mass="13570" checksum="027CEB1D461EAD29" modified="2023-06-28" version="1">MPDPAKTAAKKGSKKAVTKTAGKGGKKRRKSRKESYAIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGESSRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QK21</accession>
    <name>A0A2D0QK21_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sine oculis homeobox homolog 1a</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Sine oculis homeobox homolog 1b</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">six2a</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIX/Sine oculis homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017318559.1">
        <property type="nucleotide sequence ID" value="XM_017463070.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QK21"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000014101"/>
    <dbReference type="GeneID" id="108262766"/>
    <dbReference type="KEGG" id="ipu:108262766"/>
    <dbReference type="CTD" id="83415"/>
    <dbReference type="OMA" id="VEHQYPI"/>
    <dbReference type="OrthoDB" id="3501850at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048793">
        <property type="term" value="P:pronephros development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014857">
        <property type="term" value="P:regulation of skeletal muscle cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048741">
        <property type="term" value="P:skeletal muscle fiber development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000063">
        <property type="entry name" value="SIX homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031701">
        <property type="entry name" value="SIX1_SD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10390">
        <property type="entry name" value="HOMEOBOX PROTEIN SIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10390:SF61">
        <property type="entry name" value="HOMEOBOX PROTEIN SIX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16878">
        <property type="entry name" value="SIX1_SD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 11 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 11 15" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="122"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="124"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="167"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="245"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="179"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="200"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="258"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017318559.1"/>
        </source>
    </evidence>
    <sequence length="298" mass="33442" checksum="AEB0E738AA2A79A3" modified="2017-12-20" version="1">MSMLPTFGFTQEQVACVCEVLQQGGNIERLGRFLWSLPACEHLHKNESVLKAKAVVAFHRGNFRELYKILESHQFSPHNHPKLQQLWLKAHYIEAEKLRGRPLGAVGKYRVRRKFPLPRSIWDGEETSYCFKEKSRSVLREWYTHNPYPSPREKRELAEATGLTTTQVSNWFKNRRQRDRAAEAKERENNENSNTNSHNALTSSMNGNKTMMGSSDEDKTPIGTPDHTSSSPALLLTSASGLQPLHGLAAPPAPSAVPVPSAVTVPSADSMHHQHHHHHGLHDTILNTMASNLVDLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QLT5</accession>
    <name>A0A2D0QLT5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Fibronectin type III domain-containing protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">fndc5a</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017318676.1">
        <property type="nucleotide sequence ID" value="XM_017463187.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QLT5"/>
    <dbReference type="GeneID" id="108262834"/>
    <dbReference type="KEGG" id="ipu:108262834"/>
    <dbReference type="CTD" id="795265"/>
    <dbReference type="OMA" id="YFSCASL"/>
    <dbReference type="OrthoDB" id="5843172at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000117">
        <property type="entry name" value="Fibronectin type III domain containing 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032073">
        <property type="entry name" value="FNDC5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052120">
        <property type="entry name" value="FNDC_type_III_4/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14470">
        <property type="entry name" value="FIBRONECTIN TYPE III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14470:SF1">
        <property type="entry name" value="FIBRONECTIN TYPE III DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16066">
        <property type="entry name" value="DUF4808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012836013" evidence="10">
        <location>
            <begin position="24"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="28"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="173"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017318676.1"/>
        </source>
    </evidence>
    <sequence length="202" mass="22177" checksum="51FB62B7E5453128" modified="2017-12-20" version="1">MTPCLARAVLCVLSCFSVSRVLADSLAPPVNVSIRDLKANSATVTWDIPGAEPVIGFAITQQKKDVHMLRFIQEVNTTTRSCALWDLEAETDYIVHVQSISAHGTSPPSETLRFRTPKETEIQASKNEVTMEEVGQASQLRAGELIIIVVVLVMWAGVIALFCRQYDIIKDNEPNNNKDKAKNSSECSTPEHPTGGLLRSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QNT0</accession>
    <name>A0A2D0QNT0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Calcium homeostasis modulator protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC108263125</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CALHM family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017319105.2">
        <property type="nucleotide sequence ID" value="XM_017463616.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QNT0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000031536"/>
    <dbReference type="GeneID" id="108263125"/>
    <dbReference type="KEGG" id="ipu:108263125"/>
    <dbReference type="OMA" id="LNTHTWN"/>
    <dbReference type="OrthoDB" id="9865653at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005261">
        <property type="term" value="F:monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904669">
        <property type="term" value="P:ATP export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029569">
        <property type="entry name" value="CALHM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32261">
        <property type="entry name" value="CALCIUM HOMEOSTASIS MODULATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32261:SF3">
        <property type="entry name" value="CALCIUM HOMEOSTASIS MODULATOR PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14798">
        <property type="entry name" value="Ca_hom_mod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017319105.2"/>
        </source>
    </evidence>
    <sequence length="318" mass="35497" checksum="515AD3B6D566F737" modified="2023-02-22" version="2">MAAFIAENFKFAALFFKSKDVVIFNGLIALGTVASQTAYNIFAFECPCSAQRNYLYGLAAIGVPALTFFLIGVMLSRSTWDLVSECRIRKCRKLSAAAAFALLGSIVGRAAVAPVTWSVISLLRGEAYVCALSEFVDPQSLENFPSTTQSSIIMAQFPCKDVPSELLHFWKDIERRLKYESQLLGWMLVAIISFTVFLVMCGKRCCSPLGYQQEAYWSSYRANEHALFQRTAEAHAKLRAAESIRTFFGFVALEEQERGLLAACHGAKLAISNLEWNQITGVYLYRENEGIPLYSRLNKWANFTIGNNTEAIEMDMLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QPM5</accession>
    <name>A0A2D0QPM5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">MARVEL domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">marveld1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017319410.1">
        <property type="nucleotide sequence ID" value="XM_017463921.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QPM5"/>
    <dbReference type="GeneID" id="108263273"/>
    <dbReference type="KEGG" id="ipu:108263273"/>
    <dbReference type="CTD" id="83742"/>
    <dbReference type="OMA" id="AAHKYEG"/>
    <dbReference type="OrthoDB" id="9938733at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019911">
        <property type="term" value="F:structural constituent of myelin sheath"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042552">
        <property type="term" value="P:myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050578">
        <property type="entry name" value="MARVEL-CKLF_proteins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776:SF28">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776">
        <property type="entry name" value="MARVEL-CONTAINING POTENTIAL LIPID RAFT-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="7">
        <location>
            <begin position="14"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017319410.1"/>
        </source>
    </evidence>
    <sequence length="162" mass="18254" checksum="BCE2474C486BF033" modified="2017-12-20" version="1">MSPQPQVTRTFQEFLKSFLGIVRILQILLGSGLWITVATSKYEGSVHFVLFVAVFFWLLTLALFFLTLLNKQDLVPILGGERWLLTNVIHDFAATLLYLSAIGIMIYKTSKNSYCNLPQYKHTCLYTVYLTGSVFACLTACAYLLSAIYGSCRKCRGEQTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QR58</accession>
    <name>A0A2D0QR58_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sprouty-related, EVH1 domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">spred3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017320784.1">
        <property type="nucleotide sequence ID" value="XM_017465295.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QR58"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000950"/>
    <dbReference type="GeneID" id="108264009"/>
    <dbReference type="KEGG" id="ipu:108264009"/>
    <dbReference type="CTD" id="399473"/>
    <dbReference type="OMA" id="QFCARWA"/>
    <dbReference type="OrthoDB" id="5786858at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10574">
        <property type="entry name" value="EVH1_SPRED-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000052">
        <property type="entry name" value="Sprouty-related, EVH1 domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023337">
        <property type="entry name" value="KBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041937">
        <property type="entry name" value="SPRE_EVH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007875">
        <property type="entry name" value="Sprouty"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000697">
        <property type="entry name" value="WH1/EVH1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202:SF19">
        <property type="entry name" value="SPROUTY-RELATED, EVH1 DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202">
        <property type="entry name" value="SPROUTY-RELATED, EVH1 DOMAIN-CONTAINING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05210">
        <property type="entry name" value="Sprouty"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00568">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00461">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51488">
        <property type="entry name" value="KBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51227">
        <property type="entry name" value="SPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50229">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="476"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="WH1" evidence="6">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="KBD" evidence="7">
        <location>
            <begin position="236"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="116"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="206"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="322"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="136"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="210"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="337"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017320784.1"/>
        </source>
    </evidence>
    <sequence length="518" mass="55231" checksum="B80CD2D003F7E8C8" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QU63</accession>
    <name>A0A2D0QU63_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Vasodilator-stimulated phosphoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">vaspb</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">SDA103</strain>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">Homotetramer. Interacts with PFN1, PFN2, LPP, ACTN1 and ACTG1. Interacts, via the EVH1 domain, with the Pro-rich regions of ZYX. This interaction is important for targeting to focal adhesions and the formation of actin-rich structures at the apical surface of cells. Interacts, via the EVH1 domain, with the Pro-rich domain of Listeria monocytogenes actA. Interacts with APBB1IP. Interacts, via the Pro-rich domain, with the C-terminal SH3 domain of DNMBP. Interacts weakly with MEFV.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell junction</location>
            <location evidence="4">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Cell projection</location>
            <location evidence="19">Filopodium membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Lamellipodium membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Ena/VASP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017321266.1">
        <property type="nucleotide sequence ID" value="XM_017465777.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QU63"/>
    <dbReference type="GeneID" id="108264341"/>
    <dbReference type="CTD" id="550487"/>
    <dbReference type="OrthoDB" id="31170at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031527">
        <property type="term" value="C:filopodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031258">
        <property type="term" value="C:lamellipodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005522">
        <property type="term" value="F:profilin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001843">
        <property type="term" value="P:neural tube closure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030838">
        <property type="term" value="P:positive regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01207">
        <property type="entry name" value="EVH1_Ena_VASP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000005">
        <property type="entry name" value="vasodilator-stimulated phosphoprotein isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000047">
        <property type="entry name" value="vasodilator-stimulated phosphoprotein isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038023">
        <property type="entry name" value="VASP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014885">
        <property type="entry name" value="VASP_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000697">
        <property type="entry name" value="WH1/EVH1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202">
        <property type="entry name" value="SPROUTY-RELATED, EVH1 DOMAIN-CONTAINING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202:SF12">
        <property type="entry name" value="VASODILATOR-STIMULATED PHOSPHOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08776">
        <property type="entry name" value="VASP_tetra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00568">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00461">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118370">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein, VASP, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50229">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="16" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="11" id="KW-0965">Cell junction</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0729">SH3-binding</keyword>
    <keyword evidence="6" id="KW-0796">Tight junction</keyword>
    <feature type="domain" description="WH1" evidence="24">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="150"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="150"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="23">
        <location>
            <begin position="181"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="23">
        <location>
            <begin position="236"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00046311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00059051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00061997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00074299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_017321266.1"/>
        </source>
    </evidence>
    <sequence length="428" mass="45294" checksum="7D69BAA513CEDDA1" modified="2017-12-20" version="1">MSELSVCNARATVMVYDEAGKRWVAAGTGPQVFSRVQIYHNPSTNSFRVVGRKMQPDQQVVMNCPIVKGLKYNQATANFHQWRDARQVWGLNFGSKEDAALFANGMMHALEVLSADGGYSAGPRLVSNGPTQEELEQQRRLEQQRLEQLERERQERERQERERLERERQERERQAASVMVPPAPPLAPGGPPAPPAPPPPPGPPPSVGGGPPPPPGPPPSGGGAPPPPAPPLPSASGGGGEGGLGGGGLAAALAGAKLRKVAKDETGSAAPSAPAPAPAPAKPDLSRCSGSGPSGGGGDLFGEMSAILARRRKAADKGEKAPPAPKTEEPSNDDSESQGQSDTLRRPWEKSATMPRNNSVTRGLDSPAFTALTPRLKAAGSSNDAESGDESELERVKQELLEEVRKELQKVKDEIIGAIIQELQRRST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QU92</accession>
    <name>A0A2D0QU92_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Translation initiation factor IF-2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">mtif2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017321987.1">
        <property type="nucleotide sequence ID" value="XM_017466498.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QU92"/>
    <dbReference type="GeneID" id="108264715"/>
    <dbReference type="KEGG" id="ipu:108264715"/>
    <dbReference type="CTD" id="4528"/>
    <dbReference type="OrthoDB" id="361630at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03702">
        <property type="entry name" value="IF2_mtIF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03692">
        <property type="entry name" value="mtIF2_IVc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000007">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10050:FF:000001">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000019">
        <property type="entry name" value="Translation initiation factor IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000072">
        <property type="entry name" value="translation initiation factor IF-2, mitochondrial isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00100_B">
        <property type="entry name" value="IF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053905">
        <property type="entry name" value="EF-G-like_DII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044145">
        <property type="entry name" value="IF2_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000178">
        <property type="entry name" value="TF_IF2_bacterial-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF20">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22042">
        <property type="entry name" value="EF-G_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01176">
        <property type="entry name" value="IF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 13" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="151"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017321987.1"/>
        </source>
    </evidence>
    <sequence length="699" mass="77573" checksum="9EABA6FB853797C3" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QVW2</accession>
    <name>A0A2D0QVW2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Formin-binding protein 1a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">fnbp1a</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FNBP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017322449.2">
        <property type="nucleotide sequence ID" value="XM_017466960.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QVW2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013460"/>
    <dbReference type="GeneID" id="108264955"/>
    <dbReference type="KEGG" id="ipu:108264955"/>
    <dbReference type="CTD" id="558990"/>
    <dbReference type="OrthoDB" id="8783038at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07676">
        <property type="entry name" value="F-BAR_FBP17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12071">
        <property type="entry name" value="SH3_FBP17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000002">
        <property type="entry name" value="Formin-binding protein 1-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000017">
        <property type="entry name" value="Formin-binding protein 1-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037449">
        <property type="entry name" value="FNBP1_F-BAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035492">
        <property type="entry name" value="FNBP1_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057870">
        <property type="entry name" value="HR1_TOCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735">
        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF22">
        <property type="entry name" value="FORMIN-BINDING PROTEIN 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25610">
        <property type="entry name" value="HR1_TOCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51860">
        <property type="entry name" value="REM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 14 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="10" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="18">
        <location>
            <begin position="18"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="19">
        <location>
            <begin position="420"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="569"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="350"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="512"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="427"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="354"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="515"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="542"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017322449.2"/>
        </source>
    </evidence>
    <sequence length="640" mass="74558" checksum="DF15ECDD471EA76B" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QXL4</accession>
    <name>A0A2D0QXL4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Regulator of MON1-CCZ1 complex</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rmc1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017322491.1">
        <property type="nucleotide sequence ID" value="XM_017467002.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QXL4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000002161"/>
    <dbReference type="GeneID" id="108264975"/>
    <dbReference type="KEGG" id="ipu:108264975"/>
    <dbReference type="CTD" id="29919"/>
    <dbReference type="OMA" id="VWVHNRE"/>
    <dbReference type="OrthoDB" id="26384at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035658">
        <property type="term" value="C:Mon1-Ccz1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040371">
        <property type="entry name" value="RMC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009755">
        <property type="entry name" value="RMC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049040">
        <property type="entry name" value="RMC1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12897">
        <property type="entry name" value="COLON CANCER-ASSOCIATED PROTEIN MIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12897:SF4">
        <property type="entry name" value="REGULATOR OF MON1-CCZ1 COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07035">
        <property type="entry name" value="RMC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21029">
        <property type="entry name" value="RMC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Regulator of MON1-CCZ1 complex N-terminal" evidence="2">
        <location>
            <begin position="25"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Mic1" evidence="1">
        <location>
            <begin position="381"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017322491.1"/>
        </source>
    </evidence>
    <sequence length="655" mass="74340" checksum="14198A01A1120399" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R4U1</accession>
    <name>A0A2D0R4U1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Sodium/hydrogen exchanger</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">slc9a2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017325627.1">
        <property type="nucleotide sequence ID" value="XM_017470138.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R4U1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007014"/>
    <dbReference type="GeneID" id="108266601"/>
    <dbReference type="KEGG" id="ipu:108266601"/>
    <dbReference type="CTD" id="6549"/>
    <dbReference type="OMA" id="INMFRTI"/>
    <dbReference type="OrthoDB" id="196264at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098719">
        <property type="term" value="P:sodium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004709">
        <property type="entry name" value="NaH_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032103">
        <property type="entry name" value="NHE_CaM-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00840">
        <property type="entry name" value="b_cpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF196">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16644">
        <property type="entry name" value="NEXCaM_BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01084">
        <property type="entry name" value="NAHEXCHNGR"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0050">Antiport</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="11 12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="162"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="312"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="356"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="384"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="425"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="451"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="15">
        <location>
            <begin position="82"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/hydrogen exchanger regulatory region" evidence="16">
        <location>
            <begin position="583"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="678"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="732"/>
            <end position="745"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017325627.1"/>
        </source>
    </evidence>
    <sequence length="808" mass="92235" checksum="D77CA8D5FDAE9D14" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R5I3</accession>
    <name>A0A2D0R5I3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein CMSS1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cmss1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017325829.1">
        <property type="nucleotide sequence ID" value="XM_017470340.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R5I3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000017069"/>
    <dbReference type="GeneID" id="108266691"/>
    <dbReference type="KEGG" id="ipu:108266691"/>
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    <dbReference type="OMA" id="DHFAQKA"/>
    <dbReference type="OrthoDB" id="1929311at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032704">
        <property type="entry name" value="Cms1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24030">
        <property type="entry name" value="PROTEIN CMSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24030:SF0">
        <property type="entry name" value="PROTEIN CMSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14617">
        <property type="entry name" value="CMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017325829.1"/>
        </source>
    </evidence>
    <sequence length="290" mass="32700" checksum="9B0B9BA5E54969B4" modified="2017-12-20" version="1">MGDDLGDEWWAQGEDLGESGEEDARDTPAEPEEPEEKAAKKRKLKTQEPDEVKRKKTVQKERIVPKQKAHPEEKKEAGKPKKRRKKKTITDVLATSAPAPGSPADLRSLVRSHFSGIRSVIEQEELDLPESCFLMSNDLTHSLSSFLKEVCPKWAKVQKQHTQTRSVVLLIVCSSALRTIELIKQLTTFKGEAKVLKLFAKHIKAEDQMKLLSKSVAHIGVGTPGRLSTLIEKEGLSLQGLRYLVLDWNHRDQKQRRMVDIPEVKGELLKLMETSIIKKCQEGAVKIALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R5L1</accession>
    <name>A0A2D0R5L1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cyclin-Y-like protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ccnyl1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin Y subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017325897.1">
        <property type="nucleotide sequence ID" value="XM_017470408.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R5L1"/>
    <dbReference type="GeneID" id="108266739"/>
    <dbReference type="KEGG" id="ipu:108266739"/>
    <dbReference type="CTD" id="151195"/>
    <dbReference type="OrthoDB" id="10250320at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20540">
        <property type="entry name" value="CYCLIN_CCNY_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000011">
        <property type="entry name" value="Cyclin-Y isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012399">
        <property type="entry name" value="Cyclin_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14248">
        <property type="entry name" value="CYCLIN Y, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028934">
        <property type="entry name" value="Cyclin_CG14939"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0195">Cyclin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="172"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="53"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="53"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017325897.1"/>
        </source>
    </evidence>
    <sequence length="340" mass="38506" checksum="C353733A59B6015B" modified="2017-12-20" version="1">MIRRPIEIDGILKEAGPGGFGGGTDGGDGQRIAILAGGVEEGITRRNLKKRPDLALESNPSDHARASTIFLSKSQTDVRDKRKSNHINHVSPGPLSKKYSSCSTIFIDDSTVSQPNLKSTIKCVTLAIYYHIKNRDSDRSLDIFDEKKHPLSREQVPDDYSRTDPEHKLIYRFVRTLFSAAQLTAECAIVTLVYLERLLTYAEMDICPCNWKRIVLGAILLASKVWDDQAVWNVDYCQILKDITVEDMNEMERHFLELLQFNINVPASVYAKYYFDLRSLADDNNLNFPLEPLSNERAQKLEAISRLCEDKYKDLSRAAMRRSFSADNLVGIRRSNAVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R768</accession>
    <name>A0A2D0R768_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Dipeptidyl peptidase 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">fap</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Invadopodium membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Lamellipodium membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017326432.1">
        <property type="nucleotide sequence ID" value="XM_017470943.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R768"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019937"/>
    <dbReference type="GeneID" id="108267048"/>
    <dbReference type="KEGG" id="ipu:108267048"/>
    <dbReference type="CTD" id="2191"/>
    <dbReference type="OMA" id="MRTPQEN"/>
    <dbReference type="OrthoDB" id="16520at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031258">
        <property type="term" value="C:lamellipodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002471">
        <property type="entry name" value="Pept_S9_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF204">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00708">
        <property type="entry name" value="PRO_ENDOPEP_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="7">
        <location>
            <begin position="103"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="6">
        <location>
            <begin position="548"/>
            <end position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017326432.1"/>
        </source>
    </evidence>
    <sequence length="751" mass="85726" checksum="F99C019CF67304CB" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R7D5</accession>
    <name>A0A2D0R7D5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Activin receptor type-1</fullName>
            <ecNumber evidence="5">2.7.11.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Activin receptor type I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">LOC108266766</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">SDA103</strain>
        </source>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="29">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-seryl-[receptor-protein] + ATP = O-phospho-L-seryl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18673"/>
            <dbReference type="Rhea" id="RHEA-COMP:11022"/>
            <dbReference type="Rhea" id="RHEA-COMP:11023"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-threonyl-[receptor-protein] + ATP = O-phospho-L-threonyl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44880"/>
            <dbReference type="Rhea" id="RHEA-COMP:11024"/>
            <dbReference type="Rhea" id="RHEA-COMP:11025"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.30" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017325966.1">
        <property type="nucleotide sequence ID" value="XM_017470477.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R7D5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008138"/>
    <dbReference type="GeneID" id="108266766"/>
    <dbReference type="KEGG" id="ipu:108266766"/>
    <dbReference type="OrthoDB" id="69842at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070724">
        <property type="term" value="C:BMP receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004675">
        <property type="term" value="F:transmembrane receptor protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032924">
        <property type="term" value="P:activin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14142">
        <property type="entry name" value="STKc_ACVR1_ALK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23535">
        <property type="entry name" value="TFP_LU_ECD_ALK2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000018">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000064">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.60.10:FF:000008">
        <property type="entry name" value="Serine/threonine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000472">
        <property type="entry name" value="Activin_recp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003605">
        <property type="entry name" value="GS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000333">
        <property type="entry name" value="TGFB_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255:SF69">
        <property type="entry name" value="ACTIVIN RECEPTOR TYPE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR TYPE I AND II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01064">
        <property type="entry name" value="Activin_recp"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08515">
        <property type="entry name" value="TGF_beta_GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00467">
        <property type="entry name" value="GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51256">
        <property type="entry name" value="GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 24" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="19" id="KW-0464">Manganese</keyword>
    <keyword evidence="16 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 24" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17 29" id="KW-0675">Receptor</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 25" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="38"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="GS" evidence="27">
        <location>
            <begin position="392"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="26">
        <location>
            <begin position="422"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="449"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00039914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00041365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_017325966.1"/>
        </source>
    </evidence>
    <sequence length="723" mass="80088" checksum="BD78A29A04198AF3" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R814</accession>
    <name>A0A2D0R814_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ankyrin repeat domain-containing protein 53</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ankrd53</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017326728.2">
        <property type="nucleotide sequence ID" value="XM_017471239.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R814"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000011724"/>
    <dbReference type="GeneID" id="108267248"/>
    <dbReference type="KEGG" id="ipu:108267248"/>
    <dbReference type="CTD" id="79998"/>
    <dbReference type="OrthoDB" id="10254927at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007080">
        <property type="term" value="P:mitotic metaphase chromosome alignment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031116">
        <property type="term" value="P:positive regulation of microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902412">
        <property type="term" value="P:regulation of mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060236">
        <property type="term" value="P:regulation of mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042335">
        <property type="entry name" value="ANKRD53"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24160">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24160:SF1">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="80"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="117"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017326728.2"/>
        </source>
    </evidence>
    <sequence length="382" mass="42379" checksum="50FC721573836AEB" modified="2023-06-28" version="2">MEGVCPAGARKLPAGDLFHAAVSGDPEWLCLSLERVQSPSQQQNKQGLTVLHVAAQHGQLNCLKLLLESCTVEVNACCPHGRRPLHMVLSPESKPNSYCCLTYLLEHGAEPNVSTDEGLTPLHLAAAEGLRECVEALVRVGADTHARDSRGHTPLEYACLWGHRVVARDKKTDMEKRKELQSLKQNMIRMHRKAEGMQKLAREAINKQKVSEWAESKGLPCLRSPTTGLWTLSHHCCHSAKPVKKHHATESRDMWNISPNPSRPPPANISRSKTVRIGVHPEEEADEPDLRQSVTLHCFGNGHIHCTTSWDDIPQQLPDLPLDVIQKDLFPSEFPSRISGPLQLQCSSVLDLSHRGGTRGNEASPWTEVAMHLAEELQPGHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R9A7</accession>
    <name>A0A2D0R9A7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein 198</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">tmem198a</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM198 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017326410.1">
        <property type="nucleotide sequence ID" value="XM_017470921.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R9A7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000015484"/>
    <dbReference type="GeneID" id="108267037"/>
    <dbReference type="KEGG" id="ipu:108267037"/>
    <dbReference type="CTD" id="553226"/>
    <dbReference type="OMA" id="AYSHTRM"/>
    <dbReference type="OrthoDB" id="115781at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090263">
        <property type="term" value="P:positive regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025256">
        <property type="entry name" value="TM7S3/TM198-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040236">
        <property type="entry name" value="TMEM198"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31247:SF7">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31247">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 198 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13886">
        <property type="entry name" value="TM7S3_TM198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="214"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="TM7S3/TM198-like" evidence="9">
        <location>
            <begin position="43"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="264"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="281"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="299"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017326410.1"/>
        </source>
    </evidence>
    <sequence length="386" mass="43910" checksum="84F11AFFD5D54B6D" modified="2017-12-20" version="1">MTSMAQLLASKLAPLSQVGSRDGLFSCDEEIERRYEVVPSVVCSMCCLFGIIYCFFGYRCFKAVMFLTGLMFGSIIIFMLCYKERVMDTQLSVEASVGIGLGIGTLCGLVTMLVRSVGLFMVGLLLGLLVALASLVVLEEFYHPHTVWLPLGVLLGSGMLFAVLTLQWQRCFTTLSTAVFGSAIVTVTVDYFVELFALTRYIYERVKVSPAQPVCWYTWVVMGVWPVLALLGVLIQWKVTADGYSHTEVFISHQQRRLQLMKIRQKEERRESRKKKKKQQQKPQQQQQQQQKQKYHHPPQSNHHATNPPAKTQHQEPTYRRKPNPTRRYDGDVLSPSYIQHFRDRHVDRRGYSHGRLVSSSHTVDIDYDCGSQVPLTAHTGPAVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R9Z5</accession>
    <name>A0A2D0R9Z5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Rab effector MyRIP isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">myripa</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017327389.3">
        <property type="nucleotide sequence ID" value="XM_017471900.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R9Z5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000004829"/>
    <dbReference type="GeneID" id="108267629"/>
    <dbReference type="KEGG" id="ipu:108267629"/>
    <dbReference type="CTD" id="561671"/>
    <dbReference type="OrthoDB" id="10072397at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017022">
        <property type="term" value="F:myosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000018">
        <property type="entry name" value="Synaptotagmin-like 5, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041282">
        <property type="entry name" value="FYVE_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051745">
        <property type="entry name" value="Intracell_Transport_Effector"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006788">
        <property type="entry name" value="Myrip/Melanophilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010911">
        <property type="entry name" value="Rab_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14555">
        <property type="entry name" value="MYELIN-ASSOCIATED OLIGODENDROCYTIC BASIC PROTEIN MOBP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14555:SF6">
        <property type="entry name" value="RAB EFFECTOR MYRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02318">
        <property type="entry name" value="FYVE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04698">
        <property type="entry name" value="Rab_eff_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50916">
        <property type="entry name" value="RABBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RabBD" evidence="8">
        <location>
            <begin position="25"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="413"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="478"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="549"/>
            <end position="652"/>
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            <end position="885"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="7">
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            <begin position="1151"/>
            <end position="1180"/>
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            <begin position="43"/>
            <end position="77"/>
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        <location>
            <begin position="1110"/>
            <end position="1144"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="7">
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            <begin position="497"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017327389.3"/>
        </source>
    </evidence>
    <sequence length="1180" mass="133578" checksum="76511ECDEEA2C6A0" modified="2023-06-28" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RCM2</accession>
    <name>A0A2D0RCM2_ICTPU</name>
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        <submittedName>
            <fullName evidence="14">Tenascin-N isoform X1</fullName>
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        <name evidence="14" type="primary">tnn</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
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    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tenascin family.</text>
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        <property type="nucleotide sequence ID" value="XM_017472798.3"/>
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    <dbReference type="STRING" id="7998.ENSIPUP00000026869"/>
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    <dbReference type="KEGG" id="ipu:108268097"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="Tenascin C"/>
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        <property type="entry name" value="Tenascin isoform 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="6"/>
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        <property type="match status" value="3"/>
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        <property type="entry name" value="ECM_Regulatory_Proteins"/>
    </dbReference>
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        <property type="entry name" value="EGF"/>
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        <property type="entry name" value="Fibrinogen-like_C"/>
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        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
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        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
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        <property type="entry name" value="Fibrinogen_CS"/>
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        <property type="entry name" value="FN3_dom"/>
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        <property type="entry name" value="FN3_sf"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TENASCIN"/>
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    </dbReference>
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        <property type="entry name" value="TENASCIN N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EGF_TEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="fn3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00514">
        <property type="entry name" value="FIBRINOGEN_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012112959" evidence="10">
        <location>
            <begin position="26"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="251"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="340"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="433"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="519"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="608"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="695"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="12">
        <location>
            <begin position="783"/>
            <end position="999"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017328287.1"/>
        </source>
    </evidence>
    <sequence length="1014" mass="112137" checksum="EC17DF2CF84B2FFF" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0REY7</accession>
    <name>A0A2D0REY7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">tcirg1b</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017329102.1">
        <property type="nucleotide sequence ID" value="XM_017473613.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0REY7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019252"/>
    <dbReference type="GeneID" id="108268578"/>
    <dbReference type="KEGG" id="ipu:108268578"/>
    <dbReference type="CTD" id="406342"/>
    <dbReference type="OMA" id="QFQMITE"/>
    <dbReference type="OrthoDB" id="10264220at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026028">
        <property type="entry name" value="V-type_ATPase_116kDa_su_euka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF21">
        <property type="entry name" value="V-TYPE PROTON ATPASE 116 KDA SUBUNIT A 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001293">
        <property type="entry name" value="ATP6V0A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="401"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="446"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="540"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="568"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="638"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="761"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017329102.1"/>
        </source>
    </evidence>
    <sequence length="831" mass="94505" checksum="DA3CE605D26F69C6" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RFL5</accession>
    <name>A0A2D0RFL5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="24">Neuronal acetylcholine receptor subunit beta-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">chrnb5b</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017329329.1">
        <property type="nucleotide sequence ID" value="XM_017473840.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RFL5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027437"/>
    <dbReference type="GeneID" id="108268717"/>
    <dbReference type="KEGG" id="ipu:108268717"/>
    <dbReference type="CTD" id="569662"/>
    <dbReference type="OrthoDB" id="5975154at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007271">
        <property type="term" value="P:synaptic transmission, cholinergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19064">
        <property type="entry name" value="LGIC_TM_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000006">
        <property type="entry name" value="Cholinergic receptor nicotinic beta 2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000008">
        <property type="entry name" value="Cholinergic receptor nicotinic beta 4 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000022">
        <property type="entry name" value="Nicotinic acetylcholine receptor subunit alpha4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15 20" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 20" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="11 24" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 20" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 20" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022267059" evidence="20">
        <location>
            <begin position="21"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="229"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="452"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="21">
        <location>
            <begin position="23"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="22">
        <location>
            <begin position="235"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_017329329.1"/>
        </source>
    </evidence>
    <sequence length="486" mass="56137" checksum="3FD846723F3BCD85" modified="2017-12-20" version="1">MAPRMEKGLWLLFLTTALCAEVEERLINNLLSPERYNKLIRPAVNKSQQVTIAIQVSLAQLISVNEREQIMTTNCWLTQVWNDYRLMWNPDEYEGVMKVRLPSQHIWLPDIVLYNNADGTYEVSFYSNAVVSSNGEVAWLPPAIYKSACKIEVRNFPFDQQNCTLKFRSWTYDHTEIDLVLLSDFASRDDFKPSGEWDIVSLPGRKNEDPDDFTYLDITYDFIIRRKPLFYTINLIVPCVLITSLAILVFYLPSDCGEKITLCISVLLALTVFLLLISKIVPPTSLAVPLIGKYLMFTMVLVTFSIVTSVCVLNIHHRSPSTHTMPPWVKSFFLNHLPALLFMQRPGSSNIREKFRRKHQHRSFSENTLKREQDALSDSTFFVNEDSKTLSRRNREMQDNTEIRKRLAVKCSADMEMAVEGVRYIADKIKSEDDDEAIIEDWKYVAMVIDRLFLWIFVLVCVSGTVGLFMQPLVNSYNTQLDDNTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RGJ9</accession>
    <name>A0A2D0RGJ9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">NF-kappa-B-repressing factor isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nkrf</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CARF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017329659.3">
        <property type="nucleotide sequence ID" value="XM_017474170.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RGJ9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033444"/>
    <dbReference type="GeneID" id="108268866"/>
    <dbReference type="CTD" id="55922"/>
    <dbReference type="OrthoDB" id="29523at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02640">
        <property type="entry name" value="R3H_NRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.50:FF:000004">
        <property type="entry name" value="NFKB repressing factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058828">
        <property type="entry name" value="DSRM_CARF/NKRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034071">
        <property type="entry name" value="R3H_NRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021859">
        <property type="entry name" value="XTBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16148:SF15">
        <property type="entry name" value="NF-KAPPA-B-REPRESSING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16148">
        <property type="entry name" value="NF-KAPPA-B-REPRESSING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26535">
        <property type="entry name" value="DSRM_CARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11952">
        <property type="entry name" value="XTBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00393">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51827">
        <property type="entry name" value="XTBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XRN2-binding (XTBD)" evidence="7">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="G-patch" evidence="5">
        <location>
            <begin position="651"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="R3H" evidence="6">
        <location>
            <begin position="700"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="39"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="175"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="182"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="394"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017329659.3"/>
        </source>
    </evidence>
    <sequence length="791" mass="88435" checksum="6E0F8EFA0DE7D098" modified="2023-06-28" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RHF3</accession>
    <name>A0A2D0RHF3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein LOC108268719</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC108268719</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017329330.1">
        <property type="nucleotide sequence ID" value="XM_017473841.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RHF3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000028202"/>
    <dbReference type="GeneID" id="108268719"/>
    <dbReference type="KEGG" id="ipu:108268719"/>
    <dbReference type="OrthoDB" id="8947657at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF111">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="22"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="161"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017329330.1"/>
        </source>
    </evidence>
    <sequence length="369" mass="40794" checksum="5C8091F230506679" modified="2017-12-20" version="1">MKVPSRKFSVVWFLNSFKHLDPSTMSFMRMALRWILILSFKIIFSAQAADFNRPSFVSMKSGERVTLNCTFQDEYSKELIVWYKQTFGELPQEVGKITANIDIEMSAEFRSGFKIEKSVNGISLTILQTKKSDEGLYFCGIYTWGNSAISSGTFIAVTGKPDLNVSVFQSGVSDSVPAGASVTLQCSVLSESRSAELQVLWFRAAPPQSHPQIIYTHHNSSHQCESGSSTHTCVYNFSKNILSLSDTGTYYCAVAVCGKIIFGNGTRVQLAMNTFTDQHHKGSDSVVISLAVALAVCVVVIFAQAILNCKKQNSVKLPQGALKVNQARDAVEMNYAALNFSERKTKSGRPKKRQPQDTVYSEVIHTPVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RJ97</accession>
    <name>A0A2D0RJ97_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fibroblast growth factor 6a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fgf6a</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017330593.1">
        <property type="nucleotide sequence ID" value="XM_017475104.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RJ97"/>
    <dbReference type="GeneID" id="108269308"/>
    <dbReference type="KEGG" id="ipu:108269308"/>
    <dbReference type="CTD" id="30560"/>
    <dbReference type="OrthoDB" id="8909943at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013379511" evidence="1">
        <location>
            <begin position="21"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017330593.1"/>
        </source>
    </evidence>
    <sequence length="240" mass="28060" checksum="6137B99E3B41DBF7" modified="2017-12-20" version="1">MPSVLLLCWAVLTLHTTATCYHLQWTSLASGADPADPETHQWLEEKNYFIRKKGNKIAKKPVRRGSEGNFNYRNDQYKSCFSVLNIVKKSPAFLCLDSKGKLYTSVFDSNEVCRFRRVKRYKHYDVFHSCKGDVLLVNHRQLKIYTHNLPKLSDFVFLKRPKNRTHHRKRRSWHTDPSDPLGSEYPYLSTVRNWRSDSEHGGNVSKETITSVDDPLEVLLSHSAQTPTLEKHRENRKVRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RJN6</accession>
    <name>A0A2D0RJN6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein clcf1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">clcf1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IL-6 superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047013273.1">
        <property type="nucleotide sequence ID" value="XM_047157317.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RJN6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000023750"/>
    <dbReference type="GeneID" id="108269401"/>
    <dbReference type="KEGG" id="ipu:108269401"/>
    <dbReference type="CTD" id="23529"/>
    <dbReference type="OMA" id="VGCVQVQ"/>
    <dbReference type="OrthoDB" id="8956155at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048680">
        <property type="term" value="P:positive regulation of axon regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010681">
        <property type="entry name" value="PRF/CT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21353">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21353:SF7">
        <property type="entry name" value="CARDIOTROPHIN-LIKE CYTOKINE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043523167" evidence="6">
        <location>
            <begin position="25"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="178"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="262"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="211"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="277"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_047013273.1"/>
        </source>
    </evidence>
    <sequence length="430" mass="48713" checksum="0F133651C6B5EA1C" modified="2017-12-20" version="1">MMRREVHQAQVVLLLAAAVGCVQVQDHALMLSNERSTIERTYELTKYLNHQLKEIKDTYLSYLGPPFSDPGFSPPRPNISSLAVPSAATREDLWRGLENGVRLAQNQRAYSVLLCAVRELARSTLCPYLQSSLLHFCSGLSGLLGSISGLMNALGHTPPLHGNAMPSQKYTPLLSSAYQRENGNGPAPLRNHASRVQSGLGGTRDATAGYETDRKRDRERERAERGRRGRRREGESWTEREDEEREEGLEKWKRRRRLLTVEDEDDGKQTSHSFDPNSNNNNNMNNTHTSSKHYFSKYGKGKHYNINSTDSDARLPTETEIERRISRDEEADEFQEDVPVLFSSPSLHSGRSGRPISSPHPPLSPLSLLYAYEDGVTEVDSHLSMAAPSPRPALNDFTRKVEGFWVLRELQSWLFRSAKDFTRLKKRLRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RLL9</accession>
    <name>A0A2D0RLL9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Adenylosuccinate synthetase isozyme 1</fullName>
            <shortName evidence="11">AMPSase 1</shortName>
            <shortName evidence="11">AdSS 1</shortName>
            <ecNumber evidence="11">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Adenylosuccinate synthetase, basic isozyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Adenylosuccinate synthetase, muscle isozyme</fullName>
            <shortName evidence="11">M-type adenylosuccinate synthetase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">IMP--aspartate ligase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">adss1</name>
        <name evidence="11" type="synonym">ADSS1</name>
        <name evidence="11" type="synonym">ADSSL1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the purine nucleotide cycle (PNC), which interconverts IMP and AMP to regulate the nucleotide levels in various tissues, and which contributes to glycolysis and ammoniagenesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Component of the purine nucleotide cycle (PNC), which interconverts IMP and AMP to regulate the nucleotide levels in various tissues, and which contributes to glycolysis and ammoniagenesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Plays an important role in the de novo pathway of purine nucleotide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11 13">
            <text>IMP + L-aspartate + GTP = N(6)-(1,2-dicarboxyethyl)-AMP + GDP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11 13">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 11">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 13">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="11"/>
    <dbReference type="RefSeq" id="XP_017330845.1">
        <property type="nucleotide sequence ID" value="XM_017475356.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RLL9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000028701"/>
    <dbReference type="GeneID" id="108269458"/>
    <dbReference type="KEGG" id="ipu:108269458"/>
    <dbReference type="CTD" id="122622"/>
    <dbReference type="OMA" id="GVPFKWI"/>
    <dbReference type="OrthoDB" id="10265645at2759"/>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046040">
        <property type="term" value="P:IMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03108">
        <property type="entry name" value="AdSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.170.10:FF:000001">
        <property type="entry name" value="Adenylosuccinate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.300.10:FF:000002">
        <property type="entry name" value="Adenylosuccinate synthetase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.300.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00011">
        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03126">
        <property type="entry name" value="Adenylosucc_synth_vert_basic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018220">
        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033128">
        <property type="entry name" value="Adenylosuccin_syn_Lys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042110">
        <property type="entry name" value="Adenylosuccinate_synth_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042111">
        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027509">
        <property type="entry name" value="AdSS_1_vert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002223">
        <property type="entry name" value="PRK01117.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00184">
        <property type="entry name" value="purA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846:SF2">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE ISOZYME 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00513">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 11 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 11 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 11 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 11 13" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="40"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="41"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="66"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="68"/>
            <end position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="329"/>
            <end position="335"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="361"/>
            <end position="363"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="443"/>
            <end position="446"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017330845.1"/>
        </source>
    </evidence>
    <sequence length="455" mass="50140" checksum="3C03032E32BE2CA6" modified="2017-12-20" version="1">MSSSRSGNDHFNPINASLGAKRPRAESANKVTVVLGAQWGDEGKGKVVDLLATESDIVCRCQGGNNAGHTVVVEGTEYDFHLLPSGIINTKCISLIGNGVVIHLPGLFEEIDKNEKKGLKGWEKRLVISDRAHIVFDFHQAVDGMQEVQRQAQEGKNIGTTKKGIGPTYACKASRTGVRICDLLADFKEFSTKFKNLAQQYQSMYPALKVDVEGELKKLKDYAERIRPMVRDGVYFMYDAIHGPLKKILVEGANAALLDIDFGTYPFVTSSNCTVGGVCTGLGIPPANIGDVYGVSKAYTTRVGIGAFPTEQLNAIGELLQTRGHEVGVTTGRKRRCGWLDLVILRYAHMINGFTAIALTKLDILDVLDEIKVAVAYKLNGRRIPHFPANMEVLQKVEVEYETLPGWKTDTSAARKWNDLPPKAQNYIRFVENHIGVPIKWVGVGKSRECMIQMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RMK1</accession>
    <name>A0A2D0RMK1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Trafficking protein particle complex subunit 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Tetratricopeptide repeat protein 15</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">trappc12</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the TRAPP complex, which is involved in endoplasmic reticulum to Golgi apparatus trafficking at a very early stage. Also plays a role in chromosome congression, kinetochore assembly and stability and controls the recruitment of CENPE to the kinetochores.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the multisubunit TRAPP (transport protein particle) complex, which includes at least TRAPPC2, TRAPPC2L, TRAPPC3, TRAPPC3L, TRAPPC4, TRAPPC5, TRAPPC8, TRAPPC9, TRAPPC10, TRAPPC11 and TRAPPC12. Interacts with CENPE.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum-Golgi intermediate compartment</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017331737.2">
        <property type="nucleotide sequence ID" value="XM_017476248.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RMK1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013242"/>
    <dbReference type="GeneID" id="108270009"/>
    <dbReference type="KEGG" id="ipu:108270009"/>
    <dbReference type="CTD" id="51112"/>
    <dbReference type="OrthoDB" id="428342at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000170">
        <property type="entry name" value="Trafficking protein particle complex subunit 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21581">
        <property type="entry name" value="D-ALANYL-D-ALANINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21581:SF6">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="665"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="13">
        <location>
            <begin position="706"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="26"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="127"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="313"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="337"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00066258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00074587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00081147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017331737.2"/>
        </source>
    </evidence>
    <sequence length="821" mass="90761" checksum="14B31D77016E4057" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RP48</accession>
    <name>A0A2D0RP48_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">zdhhc3a</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="RefSeq" id="XP_017332282.1">
        <property type="nucleotide sequence ID" value="XM_017476793.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RP48"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026480"/>
    <dbReference type="GeneID" id="108270265"/>
    <dbReference type="KEGG" id="ipu:108270265"/>
    <dbReference type="CTD" id="436998"/>
    <dbReference type="OrthoDB" id="331948at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="40"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="169"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="126"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017332282.1"/>
        </source>
    </evidence>
    <sequence length="315" mass="35521" checksum="EC3ACF893414FD95" modified="2017-12-20" version="1">MRSPVPRFRDVEKQANSLQPVQCVLPCHDRQSTMWFVRDACGIVCGVITWLLVFFADFVVLFVMLIPSKNLTYSLMNGAVFNSLAFLALASHFRAMCTDPGAVPKGNATKEYIESLQLKPGQVVYKCPKCCSIKPDRAHHCSVCKRCIRKMDHHCPWVNNCVGESNQKYFVLFTMYIALISLHALFMVVLHFLYCFEDDWTKCSTFSPPATVILLILLCFEGLLFLIFTSVMFGTQVHSICTDETGIERLKGETPTWEKTSCWEAMKLAFGGPLSLAWFSPFSDLSCRTNDSSNIPEAPQGEIIAEDVIEIPLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RQC4</accession>
    <name>A0A2D0RQC4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Rho-related GTP-binding protein RhoB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC108270316</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017332371.1">
        <property type="nucleotide sequence ID" value="XM_017476882.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RQC4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012374"/>
    <dbReference type="GeneID" id="108270316"/>
    <dbReference type="KEGG" id="ipu:108270316"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01870">
        <property type="entry name" value="RhoA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000095">
        <property type="entry name" value="Rho-related GTP-binding protein RhoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
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        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
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        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RHO"/>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00057048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017332371.1"/>
        </source>
    </evidence>
    <sequence length="196" mass="21986" checksum="3FAE0C331BE71B05" modified="2017-12-20" version="1">MAAIRKKLVVVGDGACGKTCLLIVFSKDEFPEVYVPTVFENYVADIEVDSKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSVDSPDSLENIPEKWVPEVKHFCPNVPIILVANKKDLRNDENVRNELARMKQEPVKGEDGRAMAVRIGAYDYLECSAKTKEGVREVFETATRAALQKRTRPAPGCGQCCKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RRP5</accession>
    <name>A0A2D0RRP5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Protein dispatched homolog 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">disp2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the dispatched family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017332630.1">
        <property type="nucleotide sequence ID" value="XM_017477141.3"/>
    </dbReference>
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        <property type="component" value="Chromosome 9"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Dispatched RND transporter family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052081">
        <property type="entry name" value="Dispatched_Hh_regulator"/>
    </dbReference>
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        <property type="entry name" value="PTHD_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45951:SF2">
        <property type="entry name" value="PROTEIN DISPATCHED HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45951">
        <property type="entry name" value="PROTEIN DISPATCHED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Patched"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="497"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="536"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="597"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="628"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="722"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="992"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1018"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1047"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1090"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="9">
        <location>
            <begin position="518"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1406"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1434"/>
            <end position="1479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1413"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1438"/>
            <end position="1447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1448"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1468"/>
            <end position="1479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017332630.1"/>
        </source>
    </evidence>
    <sequence length="1479" mass="163986" checksum="D21A2C611F4301C2" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S0U1</accession>
    <name>A0A2D0S0U1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Somatostatin receptor type 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">si:zfos-169g10.2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017336352.1">
        <property type="nucleotide sequence ID" value="XM_017480863.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S0U1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000025577"/>
    <dbReference type="GeneID" id="108272445"/>
    <dbReference type="KEGG" id="ipu:108272445"/>
    <dbReference type="OrthoDB" id="6076970at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042923">
        <property type="term" value="F:neuropeptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004994">
        <property type="term" value="F:somatostatin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15972">
        <property type="entry name" value="7tmA_SSTR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000039">
        <property type="entry name" value="somatostatin receptor type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000586">
        <property type="entry name" value="Somatstn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001856">
        <property type="entry name" value="Somatstn_rcpt_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229">
        <property type="entry name" value="NEUROPEPTIDES RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229:SF77">
        <property type="entry name" value="SI:ZFOS-169G10.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00246">
        <property type="entry name" value="SOMATOSTATNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00589">
        <property type="entry name" value="SOMATOSTTN3R"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="66"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="226"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="275"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="311"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="82"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017336352.1"/>
        </source>
    </evidence>
    <sequence length="497" mass="55389" checksum="8702605A89BAD88E" modified="2017-12-20" version="1">METPNRIPVSGFTKPWGNNSVLSGNLGLSQATVSPPPPNDTFLLNGSLQNWTESYNTGFMPSVAGILIPLIYIIVCVVGLGGNTLVIHIVLRYSQTQSVTNIYILNLAIADELFMLGLPFLAVQNALLSWPFGSLMCRLVMTVDAINQFTSIFCLTVMSIDRYLAVVHPLRSSRWRQPRVAKAVNATVWAISFVVVLPVVVFADVLEDDGNCSIVWPEPAEVWKAAFIVYTATVGFFGPLLVICMCYLLIVIKVRSSGRRVRATSIRRRKSERKITRMVVIVVAVFVFCWLPFYILNIVNLLVLLPGEFRGLYYFVVVLSYANSCANPILYGFLSDNFKRGFRKALCRSTRRVENQDLQQGTVGNHTLPLEEMKKDLELTDCLKESCKQIQQNCVEEEEEVQEADMGYMENGTQMTEICRSVKNGCRHRRCEGTKTIVSCMVDAKEAGPSNGNRTGENGDYISPTFGHVVSFNGSQRGSIRPVAEELGDTVLEISYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S6V0</accession>
    <name>A0A2D0S6V0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein buc2l isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">buc2l</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017338161.1">
        <property type="nucleotide sequence ID" value="XM_017482672.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S6V0"/>
    <dbReference type="GeneID" id="108273451"/>
    <dbReference type="KEGG" id="ipu:108273451"/>
    <dbReference type="CTD" id="100537720"/>
    <dbReference type="OrthoDB" id="9946561at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053309">
        <property type="entry name" value="Balbiani_Body_Formation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38654:SF1">
        <property type="entry name" value="BUCKY BALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38654">
        <property type="entry name" value="BUCKY BALL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="478"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="716"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="495"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017338161.1"/>
        </source>
    </evidence>
    <sequence length="762" mass="83815" checksum="53E57F04ABD3D488" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S9V4</accession>
    <name>A0A2D0S9V4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">DNA repair endonuclease XPF</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">DNA excision repair protein ERCC-4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">ercc4</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Catalytic component of a structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair, and which is essential for nucleotide excision repair (NER) and interstrand cross-link (ICL) repair.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="18">Heterodimer composed of ERCC1 and ERCC4/XPF. Interacts with SLX4/BTBD12; this interaction is direct and links the ERCC1-ERCC4/XPF complex to SLX4, which may coordinate the action of the structure-specific endonuclease during DNA repair.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the XPF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017339020.1">
        <property type="nucleotide sequence ID" value="XM_017483531.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S9V4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000002451"/>
    <dbReference type="GeneID" id="108273887"/>
    <dbReference type="KEGG" id="ipu:108273887"/>
    <dbReference type="CTD" id="2072"/>
    <dbReference type="OMA" id="THILDIM"/>
    <dbReference type="OrthoDB" id="361020at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901255">
        <property type="term" value="P:nucleotide-excision repair involved in interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009411">
        <property type="term" value="P:response to UV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20078">
        <property type="entry name" value="XPF_nuclease_XPF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10130:FF:000002">
        <property type="entry name" value="DNA repair endonuclease XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000040">
        <property type="entry name" value="DNA repair endonuclease XPF isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006167">
        <property type="entry name" value="XPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047520">
        <property type="entry name" value="XPF_nuclease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00596">
        <property type="entry name" value="rad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150:SF0">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 24" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ERCC4" evidence="22">
        <location>
            <begin position="663"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="434"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="486"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="622"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="451"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00060353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00064560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00072370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00078823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_017339020.1"/>
        </source>
    </evidence>
    <sequence length="899" mass="102019" checksum="F55B5C4AA84DF448" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SAP3</accession>
    <name>A0A2D0SAP3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Pseudokinase FAM20A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">fam20a</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM20 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017339310.1">
        <property type="nucleotide sequence ID" value="XM_017483821.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SAP3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020934"/>
    <dbReference type="GeneID" id="108274039"/>
    <dbReference type="KEGG" id="ipu:108274039"/>
    <dbReference type="CTD" id="54757"/>
    <dbReference type="OMA" id="PLTQCCI"/>
    <dbReference type="OrthoDB" id="8583677at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070166">
        <property type="term" value="P:enamel mineralization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024869">
        <property type="entry name" value="FAM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009581">
        <property type="entry name" value="FAM20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12450">
        <property type="entry name" value="DENTIN MATRIX PROTEIN 4 PROTEIN FAM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12450:SF12">
        <property type="entry name" value="PSEUDOKINASE FAM20A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06702">
        <property type="entry name" value="Fam20C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="FAM20 C-terminal" evidence="10">
        <location>
            <begin position="289"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="394"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="325"/>
            <end position="328"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="399"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="414"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="414"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017339310.1"/>
        </source>
    </evidence>
    <sequence length="528" mass="61037" checksum="A737ED73F87C26D8" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SC44</accession>
    <name>A0A2D0SC44_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Parathyroid hormone-like hormone b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pthlhb</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the parathyroid hormone family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017340299.2">
        <property type="nucleotide sequence ID" value="XM_017484810.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SC44"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034426"/>
    <dbReference type="GeneID" id="108274596"/>
    <dbReference type="KEGG" id="ipu:108274596"/>
    <dbReference type="CTD" id="751101"/>
    <dbReference type="OrthoDB" id="9892514at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030282">
        <property type="term" value="P:bone mineralization"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="PTH-rel"/>
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        <property type="entry name" value="PTH/PTH-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17223">
        <property type="entry name" value="PARATHYROID HORMONE-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="PARATHYROID HORMONE-RELATED PROTEIN"/>
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        <property type="entry name" value="Parathyroid"/>
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    </dbReference>
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        <property type="entry name" value="PTH"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039902601" evidence="6">
        <location>
            <begin position="22"/>
            <end position="159"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017340299.2"/>
        </source>
    </evidence>
    <sequence length="159" mass="18183" checksum="8C753B737665D22B" modified="2023-06-28" version="2">MMLRQWSFAVLLLSIPLPVQGQPIHALSNRMRRSVSQAQLMHDRGRYLQDRKRRLWLQELFEQVHTANVWDAPSSSGANLQHVTWGAQAPKTGSSTKDFLLDFQLESTGTIDILPQETNKEQPAATKRKKRVCLGKWRESNKRRGWPCSIQAQVALSAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SDA0</accession>
    <name>A0A2D0SDA0_ICTPU</name>
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        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="18 19" type="primary">tdrd12</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18 19" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18 19">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="RefSeq" id="XP_017340230.2">
        <property type="nucleotide sequence ID" value="XM_017484741.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053541982.1">
        <property type="nucleotide sequence ID" value="XM_053686007.1"/>
    </dbReference>
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    <dbReference type="STRING" id="7998.ENSIPUP00000024935"/>
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    <dbReference type="KEGG" id="ipu:108274529"/>
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    <dbReference type="OrthoDB" id="249932at2759"/>
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        <property type="component" value="Chromosome 14"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:nucleic acid binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:RNA helicase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042078">
        <property type="term" value="P:germ-line stem cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Tudor_TDRD12_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tudor domain containing 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22655">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE TDRD12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22655:SF2">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE TDRD12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7 18 19" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0469">Meiosis</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="9" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Tudor" evidence="16">
        <location>
            <begin position="63"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="15">
        <location>
            <begin position="882"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="13">
        <location>
            <begin position="882"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1174"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1221"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1187"/>
            <end position="1197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017340230.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_053541982.1"/>
        </source>
    </evidence>
    <sequence length="1242" mass="138501" checksum="800ADEA30EC907B7" modified="2023-02-22" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SEL2</accession>
    <name>A0A2D0SEL2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Plakophilin-3a isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pkp3a</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta-catenin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017341159.1">
        <property type="nucleotide sequence ID" value="XM_017485670.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SEL2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033018"/>
    <dbReference type="GeneID" id="108275089"/>
    <dbReference type="CTD" id="368397"/>
    <dbReference type="OMA" id="SAAHYAM"/>
    <dbReference type="OrthoDB" id="3245100at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028435">
        <property type="entry name" value="Plakophilin/d_Catenin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10372:SF1">
        <property type="entry name" value="PLAKOPHILIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10372">
        <property type="entry name" value="PLAKOPHILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="5" id="KW-0965">Cell junction</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="423"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="465"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="106"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017341159.1"/>
        </source>
    </evidence>
    <sequence length="859" mass="93916" checksum="F1E03DB45D67D461" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SF10</accession>
    <name>A0A2D0SF10_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Carboxylesterase 1D isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ces2b</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017341304.1">
        <property type="nucleotide sequence ID" value="XM_017485815.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SF10"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006063"/>
    <dbReference type="GeneID" id="108275184"/>
    <dbReference type="KEGG" id="ipu:108275184"/>
    <dbReference type="CTD" id="234669"/>
    <dbReference type="OrthoDB" id="3200163at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00312">
        <property type="entry name" value="Esterase_lipase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000011">
        <property type="entry name" value="Carboxylic ester hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050309">
        <property type="entry name" value="Type-B_Carboxylest/Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11559">
        <property type="entry name" value="CARBOXYLESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013311187" evidence="4">
        <location>
            <begin position="23"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="5">
        <location>
            <begin position="25"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="5">
        <location>
            <begin position="550"/>
            <end position="1060"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017341304.1"/>
        </source>
    </evidence>
    <sequence length="1081" mass="118231" checksum="B154F0C67F83821A" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SHV2</accession>
    <name>A0A2D0SHV2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">dhx35</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">May be involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017342287.1">
        <property type="nucleotide sequence ID" value="XM_017486798.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SHV2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000024274"/>
    <dbReference type="GeneID" id="108275800"/>
    <dbReference type="KEGG" id="ipu:108275800"/>
    <dbReference type="CTD" id="60625"/>
    <dbReference type="OMA" id="FHEVMET"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17980">
        <property type="entry name" value="DEXHc_DHX35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000007">
        <property type="entry name" value="Probable ATP-dependent RNA helicase DHX35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000578">
        <property type="entry name" value="probable ATP-dependent RNA helicase DHX35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000767">
        <property type="entry name" value="Putative ATP-dependent RNA helicase DHX35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF136">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX35-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="62"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="259"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00058987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017342287.1"/>
        </source>
    </evidence>
    <sequence length="699" mass="78472" checksum="C85FB20FB6F70D21" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SPL4</accession>
    <name>A0A2D0SPL4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="10">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">wrn</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 18">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="10"/>
    <dbReference type="RefSeq" id="XP_017344220.1">
        <property type="nucleotide sequence ID" value="XM_017488731.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000002921"/>
    <dbReference type="GeneID" id="108276782"/>
    <dbReference type="KEGG" id="ipu:108276782"/>
    <dbReference type="CTD" id="7486"/>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18017">
        <property type="entry name" value="DEXHc_RecQ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18794">
        <property type="entry name" value="SF2_C_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06141">
        <property type="entry name" value="WRN_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.80:FF:000005">
        <property type="entry name" value="Werner syndrome ATP-dependent helicase homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000941">
        <property type="entry name" value="Werner syndrome RecQ like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001023">
        <property type="entry name" value="Werner syndrome RecQ like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="entry name" value="HRDC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004589">
        <property type="entry name" value="DNA_helicase_ATP-dep_RecQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029491">
        <property type="entry name" value="Helicase_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002121">
        <property type="entry name" value="HRDC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032284">
        <property type="entry name" value="RecQ_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018982">
        <property type="entry name" value="RQC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00614">
        <property type="entry name" value="recQ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF120">
        <property type="entry name" value="BIFUNCTIONAL 3'-5' EXONUCLEASE_ATP-DEPENDENT HELICASE WRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00570">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14493">
        <property type="entry name" value="HTH_40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16124">
        <property type="entry name" value="RecQ_Zn_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09382">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00341">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00956">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50967">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="17" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 17" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="17" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="481"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="672"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="domain" description="HRDC" evidence="13">
        <location>
            <begin position="1070"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1032"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1381"/>
            <end position="1429"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="408"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1384"/>
            <end position="1396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1419"/>
            <end position="1429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017344220.1"/>
        </source>
    </evidence>
    <sequence length="1429" mass="159581" checksum="2BD793BD5A06FB09" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SQ62</accession>
    <name>A0A2D0SQ62_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5 6">Spindlin-Z</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5 6" type="primary">LOC108277052</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4 5 6" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4 5 6">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPIN/STSY family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017344837.1">
        <property type="nucleotide sequence ID" value="XM_017489348.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017344838.1">
        <property type="nucleotide sequence ID" value="XM_017489349.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017344839.1">
        <property type="nucleotide sequence ID" value="XM_017489350.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SQ62"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008826"/>
    <dbReference type="GeneID" id="108277052"/>
    <dbReference type="KEGG" id="ipu:108277052"/>
    <dbReference type="CTD" id="792822"/>
    <dbReference type="GeneTree" id="ENSGT00950000182925"/>
    <dbReference type="OMA" id="CMCEYRK"/>
    <dbReference type="OrthoDB" id="9944558at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007276">
        <property type="term" value="P:gamete generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.70:FF:000001">
        <property type="entry name" value="Spindlin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.70">
        <property type="entry name" value="Spindlin/Ssty"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003671">
        <property type="entry name" value="SPIN/Ssty"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042567">
        <property type="entry name" value="SPIN/Ssty_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10405">
        <property type="entry name" value="SPINDLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02513">
        <property type="entry name" value="Spin-Ssty"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017344837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017344838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017344839.1"/>
        </source>
    </evidence>
    <sequence length="261" mass="29486" checksum="93037403E1E4E918" modified="2017-12-20" version="1">MKTPFGKNAGQRSRADAGHAGVSANMMKKKNSHKKHKSSVGPTKVSQPRRNIVGCRIQHIWKEGSGAASQWKGTVLDQVPVNPSLYLIKYDGFDCVYGLELHKDERVQGLEVLPDRVASTRISDAHLADTMIGKAVEHMFETEEGSKDEWRGMVLARAPIMNTWFYITYEKDPVLYMYQLLDDYKDGDLRIMPDSNDSPPAEREPGEVVDSLVGKQVEYAKEDGSKRTGMVIHQVEAKPSVYFIKFDDDFHIYVYDLVKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SR34</accession>
    <name>A0A2D0SR34_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RING finger protein 208</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rnf208</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017345154.1">
        <property type="nucleotide sequence ID" value="XM_017489665.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SR34"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033465"/>
    <dbReference type="GeneID" id="108277176"/>
    <dbReference type="KEGG" id="ipu:108277176"/>
    <dbReference type="CTD" id="727800"/>
    <dbReference type="OrthoDB" id="342730at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16559">
        <property type="entry name" value="RING-HC_RNF208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051435">
        <property type="entry name" value="RING_finger_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040100">
        <property type="entry name" value="RNF208_RING-HC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22791:SF3">
        <property type="entry name" value="RING FINGER PROTEIN 208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22791">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="147"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017345154.1"/>
        </source>
    </evidence>
    <sequence length="262" mass="28802" checksum="D1D7BDD2336AA183" modified="2017-12-20" version="1">MSCLRRQPVSIPMDTVKIIQSEKFPRECPVPVTQPRFAPPPRVAWDGGGEGEVIVNQACSDLSVEVNCNTMVNPRPLASPPAPVLRREASYLAQRKASNSEICYHQFHYKMDDVIVNQYVLRSSSTSSSSSSSTSSGPVMPCEPLDCPTCGHTYNFAGKRPRILSCLHSVCEECLQILYESCPKYKFISCPTCRRETVLFTDYGLAALAINTSILSRLPSEPGGTVQWGGDADRSCYQTVRQYCQSACTCHIANPLSTCGIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SR58</accession>
    <name>A0A2D0SR58_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">receptor protein serine/threonine kinase</fullName>
            <ecNumber evidence="5">2.7.11.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">bmpr1bb</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">SDA103</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="27">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.30" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017344780.1">
        <property type="nucleotide sequence ID" value="XM_017489291.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SR58"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006921"/>
    <dbReference type="GeneID" id="108277027"/>
    <dbReference type="KEGG" id="ipu:108277027"/>
    <dbReference type="CTD" id="100149664"/>
    <dbReference type="OrthoDB" id="69842at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004675">
        <property type="term" value="F:transmembrane receptor protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071363">
        <property type="term" value="P:cellular response to growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23613">
        <property type="entry name" value="TFP_LU_ECD_BMPR1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000018">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.60.10:FF:000001">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000055">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000472">
        <property type="entry name" value="Activin_recp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003605">
        <property type="entry name" value="GS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000333">
        <property type="entry name" value="TGFB_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255:SF62">
        <property type="entry name" value="BONE MORPHOGENETIC PROTEIN RECEPTOR TYPE-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR TYPE I AND II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01064">
        <property type="entry name" value="Activin_recp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08515">
        <property type="entry name" value="TGF_beta_GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00467">
        <property type="entry name" value="GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51256">
        <property type="entry name" value="GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="20" id="KW-0464">Manganese</keyword>
    <keyword evidence="17 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19 27" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="GS" evidence="25">
        <location>
            <begin position="273"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="303"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_017344780.1"/>
        </source>
    </evidence>
    <sequence length="601" mass="68088" checksum="41501F9947CD1A2B" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SRB6</accession>
    <name>A0A2D0SRB6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Boophilin-G2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">wu:fb59d01</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the venom Kunitz-type family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017344840.1">
        <property type="nucleotide sequence ID" value="XM_017489351.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SRB6"/>
    <dbReference type="GeneID" id="108277053"/>
    <dbReference type="KEGG" id="ipu:108277053"/>
    <dbReference type="CTD" id="322379"/>
    <dbReference type="OMA" id="FENKENC"/>
    <dbReference type="OrthoDB" id="196393at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00109">
        <property type="entry name" value="Kunitz-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22593">
        <property type="entry name" value="Kunitz_conkunitzin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000020">
        <property type="entry name" value="Collagen, type VI, alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050098">
        <property type="entry name" value="TFPI/VKTCI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083:SF217">
        <property type="entry name" value="BOOPHILIN-H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083">
        <property type="entry name" value="KUNITZ-TYPE PROTEASE INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0800">Toxin</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012293887" evidence="7">
        <location>
            <begin position="20"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="8">
        <location>
            <begin position="29"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="8">
        <location>
            <begin position="94"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017344840.1"/>
        </source>
    </evidence>
    <sequence length="210" mass="23614" checksum="0B5EF9BAEEF6493A" modified="2017-12-20" version="1">MVSRLYFPFFLMGIMCAGAQELDVKHPGCTEMMNEGTGKENTLQFYYNEETQLCIPFFYKGEGGNSNRFTTELQCLEKCSSQYRELYPEGDAVCTLDMDPGSCFARLLRYYYSKEEKVCRLFLYGGCQGNGNRFDSKEACESMCRARSGRTFGQASNPDEQTVDMGLIVGILGGVVFAVAMIATIALFVVQRKAKHTNMKKVPTTDVELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SRV2</accession>
    <name>A0A2D0SRV2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Neuropeptide B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC108277132</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the neuropeptide B/W family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017345035.1">
        <property type="nucleotide sequence ID" value="XM_017489546.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SRV2"/>
    <dbReference type="GeneID" id="108277132"/>
    <dbReference type="KEGG" id="ipu:108277132"/>
    <dbReference type="OrthoDB" id="9942334at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007631">
        <property type="term" value="P:feeding behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013297">
        <property type="entry name" value="Neuropept_BW_pre"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28553">
        <property type="entry name" value="NEUROPEPTIDE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28553:SF2">
        <property type="entry name" value="NEUROPEPTIDE W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15180">
        <property type="entry name" value="NPBW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01888">
        <property type="entry name" value="NROPEPTIDEBW"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="8" id="KW-0527">Neuropeptide</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012474701" evidence="6">
        <location>
            <begin position="26"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017345035.1"/>
        </source>
    </evidence>
    <sequence length="138" mass="15080" checksum="C38588B91592D32E" modified="2017-12-20" version="1">MGKFDERALLFLAICVLAVHTPAEAWYKQAAGPSYYSVGRASGLLSGIRRSAIRRDEAETRDSGETTENNAVIQINSRNFALKKMQSPRTNHQALSLCAPSESSELFFQHHAGTNCACANRLPAQIAQTGSCEFSLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0ST14</accession>
    <name>A0A2D0ST14_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 20">Neuromodulin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17 20">Axonal membrane protein GAP-43</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18 20">Growth-associated protein 43</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">gap43</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16 20">This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="20">Cell membrane</location>
            <topology evidence="20">Peripheral membrane protein</topology>
            <orientation evidence="20">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 20">Cell projection</location>
            <location evidence="1 20">Growth cone membrane</location>
            <topology evidence="1 20">Peripheral membrane protein</topology>
            <orientation evidence="1 20">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19 20">Synapse</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Cell projection</location>
            <location evidence="20">Filopodium membrane</location>
            <topology evidence="20">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="20">Palmitoylated. Palmitoylation is essential for plasma membrane association.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 20">Belongs to the neuromodulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017345837.1">
        <property type="nucleotide sequence ID" value="XM_017490348.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0ST14"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029127"/>
    <dbReference type="GeneID" id="108277523"/>
    <dbReference type="KEGG" id="ipu:108277523"/>
    <dbReference type="CTD" id="2596"/>
    <dbReference type="OMA" id="HFPFISK"/>
    <dbReference type="OrthoDB" id="9397439at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031527">
        <property type="term" value="C:filopodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032584">
        <property type="term" value="C:growth cone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040008">
        <property type="term" value="P:regulation of growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001422">
        <property type="entry name" value="Neuromodulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018947">
        <property type="entry name" value="Neuromodulin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033137">
        <property type="entry name" value="Neuromodulin_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018243">
        <property type="entry name" value="Neuromodulin_palmitoyl_site"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10580">
        <property type="entry name" value="Neuromodulin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00215">
        <property type="entry name" value="NEUROMODULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00412">
        <property type="entry name" value="NEUROMODULIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00413">
        <property type="entry name" value="NEUROMODULIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 20" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5 20" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14 20" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4 20" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8 20" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7 20" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="15 20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 20" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="13 20" id="KW-0564">Palmitate</keyword>
    <keyword evidence="6 20" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 20" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="Neuromodulin N-terminal" evidence="22">
        <location>
            <begin position="2"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="9"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="66"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="121"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00025215"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00030597"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00033250"/>
        </source>
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        <source>
            <dbReference type="RuleBase" id="RU368113"/>
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    </evidence>
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            <dbReference type="Pfam" id="PF10580"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017345837.1"/>
        </source>
    </evidence>
    <sequence length="222" mass="24124" checksum="10BAE41EC4167976" modified="2017-12-20" version="1">MLCCIRRTKPVEKTEEADQEIKQDGTKPEDKAHKAATKIQASFRGHIIRKKMKDDDKEEDSPAAEAKAEDNEGEKKEEKATSPTVDKQDEAAKEEEKAKSPTNSPAAEESKAEAPATSPSEPKKDVKEEPKEAEKPKEAESADVEAEKPSENVDAEKKEEGNEEEKKAEAKQADVPVTASETADCEAEVNQTQDKPDAAEDSEQAEGDVSGAANEEDKAECA</sequence>
</entry>
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    <accession>A0A2D0SWG3</accession>
    <name>A0A2D0SWG3_ICTPU</name>
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        <submittedName>
            <fullName evidence="4">Protein moonraker isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">kiaa0753</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
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            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <tissue evidence="4">Blood</tissue>
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        <property type="term" value="P:centriole replication"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="MNR"/>
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        <property type="entry name" value="PROTEIN MOONRAKER"/>
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        <property type="entry name" value="MNR"/>
        <property type="match status" value="3"/>
    </dbReference>
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        </location>
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        </location>
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        <location>
            <begin position="540"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017347019.2"/>
        </source>
    </evidence>
    <sequence length="857" mass="95982" checksum="EB5EE54968929281" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SX10</accession>
    <name>A0A2D0SX10_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Multiple epidermal growth factor-like domains protein 6 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">megf6</name>
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    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="nucleotide sequence ID" value="XM_017491735.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A2D0SX10"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027632"/>
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    <dbReference type="CTD" id="1953"/>
    <dbReference type="OrthoDB" id="409374at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
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        <property type="entry name" value="Multiple epidermal growth factor-like domains 10"/>
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        <property type="entry name" value="Predicted protein"/>
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        <property type="entry name" value="Pro-epidermal growth factor"/>
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        <property type="entry name" value="Signal peptide, CUB domain and EGF-like domain-containing 2"/>
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        <property type="entry name" value="Tyrosine protein kinase receptor tie-1, putative"/>
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        <property type="entry name" value="vitamin K-dependent protein S"/>
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        <property type="entry name" value="Vitamin K-dependent protein S"/>
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        <property type="entry name" value="Laminin"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
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        <property type="entry name" value="cEGF"/>
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    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
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        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
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        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
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        <property type="entry name" value="EGF_Ca-bd_CS"/>
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        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
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        <property type="entry name" value="MEGF/SIB"/>
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        <property type="entry name" value="NOTCH1_EGF-like"/>
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        <property type="entry name" value="MULTIPLE EPIDERMAL GROWTH FACTOR-LIKE DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24035:SF138">
        <property type="entry name" value="MULTIPLE EPIDERMAL GROWTH FACTOR-LIKE DOMAINS PROTEIN 6"/>
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    </dbReference>
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    </dbReference>
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        <location>
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        </location>
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        <location>
            <begin position="141"/>
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    <feature type="domain" description="EGF-like" evidence="6">
        <location>
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    <feature type="domain" description="EGF-like" evidence="6">
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        </location>
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    <feature type="domain" description="EGF-like" evidence="6">
        <location>
            <begin position="471"/>
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    <feature type="domain" description="EGF-like" evidence="6">
        <location>
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    <feature type="domain" description="EGF-like" evidence="6">
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    <feature type="domain" description="EGF-like" evidence="6">
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    <feature type="domain" description="EGF-like" evidence="6">
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        <location>
            <begin position="1073"/>
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        <location>
            <begin position="166"/>
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        <location>
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        <location>
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    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="1098"/>
            <end position="1107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017347224.1"/>
        </source>
    </evidence>
    <sequence length="1211" mass="131468" checksum="6D10FCA8EDF8AF1A" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T2A6</accession>
    <name>A0A2D0T2A6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Migration and invasion enhancer 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">selenow2a</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Increases cell migration by inducing filopodia formation at the leading edge of migrating cells. Plays a role in regulation of apoptosis, possibly through control of CASP3. May be involved in a redox-related process.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with GPX1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
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        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the SelWTH family.</text>
    </comment>
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    <dbReference type="OrthoDB" id="10048028at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051491">
        <property type="term" value="P:positive regulation of filopodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="migration and invasion enhancer 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011893">
        <property type="entry name" value="Selenoprotein_Rdx-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051441">
        <property type="entry name" value="SelW_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02174">
        <property type="entry name" value="CXXU_selWTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15124:SF27">
        <property type="entry name" value="MIGRATION AND INVASION ENHANCER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15124">
        <property type="entry name" value="SELENOPROTEIN W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10262">
        <property type="entry name" value="Rdx"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0711">Selenium</keyword>
    <keyword evidence="17" id="KW-0712">Selenocysteine</keyword>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="17">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00065658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00069166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017349058.1"/>
        </source>
    </evidence>
    <sequence length="95" mass="10671" checksum="04D39E932B7A2318" modified="2017-12-20" version="1">MGVQIRVEYCGGUGYEPRYQELKRVVVAEFPEAEVSGFVGRQGSFEIEINGQLVFSKLETSGFPYEDDVMEAIQKAHDGKPVEKIIKSRPPCVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T349</accession>
    <name>A0A2D0T349_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Cyclin-dependent kinase 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC108279348</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017349020.1">
        <property type="nucleotide sequence ID" value="XM_017493531.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T349"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000031698"/>
    <dbReference type="GeneID" id="108279348"/>
    <dbReference type="KEGG" id="ipu:108279348"/>
    <dbReference type="OrthoDB" id="204883at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07865">
        <property type="entry name" value="STKc_CDK9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000203">
        <property type="entry name" value="Cyclin-dependent kinase 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000227">
        <property type="entry name" value="Cyclin-dependent kinase 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="41"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="365"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="374"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017349020.1"/>
        </source>
    </evidence>
    <sequence length="394" mass="45188" checksum="B4068832593B0099" modified="2017-12-20" version="1">MQRDKTGSSAVSGDKPDRETAIMSKYYDGVEFPFCDEFSKYEKLAKIGQGTFGEVFKAKHRQTGKKVALKKVLMENEKEGFPITALREIKILQLLKHENVVNLIEICRTKATQFNRYKGSIYLVFDFCEHELAGLLSNANVKFTLSEIKKVMQMLLNGLYWIHRNKILHRDMKAANVLITRDGVLKLADFGLARAFSLAKNSQGSCYTNRVVTLWYRPPELLLGERDYGPPIDLWGAGCIMAEMWTRSPIMQGNTEQHQLTLISQLCGSITAEVWPGVDKKYELYQKMELPKGQKRKVKERLKAYVKDPYALDLIDKLLVLDPAQRVDSDDALNHDFFWSDPMPSDLKHMLSTHNTSMFEYLAPPRRRGHMPQQPANQNRNPATASQSEFDRVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T3K2</accession>
    <name>A0A2D0T3K2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Interferon-induced GTP-binding protein Mx1-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC108279589</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017349396.3">
        <property type="nucleotide sequence ID" value="XM_017493907.3"/>
    </dbReference>
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    <dbReference type="KEGG" id="ipu:108279589"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:microtubule"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
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    </dbReference>
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        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016185">
        <property type="term" value="P:synaptic vesicle budding from presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1240:FF:000007">
        <property type="entry name" value="Interferon-induced GTP-binding protein Mx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003130">
        <property type="entry name" value="GED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF225">
        <property type="entry name" value="INTERFERON-INDUCED GTP-BINDING PROTEIN MX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02212">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00302">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="6">
        <location>
            <begin position="31"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="5">
        <location>
            <begin position="544"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017349396.3"/>
        </source>
    </evidence>
    <sequence length="629" mass="72591" checksum="929111068ECFDA84" modified="2023-06-28" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T3Q1</accession>
    <name>A0A2D0T3Q1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Sodium-coupled monocarboxylate transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">slc5a8</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017349538.2">
        <property type="nucleotide sequence ID" value="XM_017494049.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T3Q1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020176"/>
    <dbReference type="GeneID" id="108279688"/>
    <dbReference type="KEGG" id="ipu:108279688"/>
    <dbReference type="CTD" id="160728"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005343">
        <property type="term" value="F:organic acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015730">
        <property type="term" value="P:propanoate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051163">
        <property type="entry name" value="Sodium:Solute_Symporter_SSF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985:SF10">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="83"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="130"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="281"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="413"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="441"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="525"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017349538.2"/>
        </source>
    </evidence>
    <sequence length="618" mass="67330" checksum="E5D751E1A1726CD6" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T578</accession>
    <name>A0A2D0T578_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TBC1 domain family member 30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tbc1d30</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May act as a GTPase-activating protein for Rab family protein(s).</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017350062.1">
        <property type="nucleotide sequence ID" value="XM_017494573.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T578"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019429"/>
    <dbReference type="GeneID" id="108279925"/>
    <dbReference type="KEGG" id="ipu:108279925"/>
    <dbReference type="CTD" id="23329"/>
    <dbReference type="OrthoDB" id="289721at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000011">
        <property type="entry name" value="TBC1 domain family member 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000009">
        <property type="entry name" value="TBC1 domain family member 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032738">
        <property type="entry name" value="Tbc1d30_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13399:SF4">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13399">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN TRAP , GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15733">
        <property type="entry name" value="DUF4682"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="4">
        <location>
            <begin position="259"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="604"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="724"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="774"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="862"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="737"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="782"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="804"/>
            <end position="817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00043879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00067508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017350062.1"/>
        </source>
    </evidence>
    <sequence length="990" mass="110998" checksum="384DC4744C411C85" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T7S5</accession>
    <name>A0A2D0T7S5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="29">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="29">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="35" type="primary">lyn</name>
    </gene>
    <organism evidence="34 35">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="34">SDA103</strain>
        </source>
    </reference>
    <reference evidence="35" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="35">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="29">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="29"/>
    <dbReference type="RefSeq" id="XP_017350896.1">
        <property type="nucleotide sequence ID" value="XM_017495407.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T7S5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003695"/>
    <dbReference type="GeneID" id="108280443"/>
    <dbReference type="KEGG" id="ipu:108280443"/>
    <dbReference type="CTD" id="4067"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="CDD" id="cd12004">
        <property type="entry name" value="SH3_Lyn"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="SH2 domain"/>
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        <property type="entry name" value="SH3 Domains"/>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="Lyn_SH3"/>
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        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
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        <property type="entry name" value="SH2"/>
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        <property type="entry name" value="SH2_dom_sf"/>
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    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
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        <property type="entry name" value="TYRKINASE"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH2 domain"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 28 29" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="17" id="KW-0391">Immunity</keyword>
    <keyword evidence="22" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="10" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="14 29 35" id="KW-0418">Kinase</keyword>
    <keyword evidence="24" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0519">Myristate</keyword>
    <keyword evidence="13 28 29" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="26" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="6 27" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="11 29" id="KW-0808">Transferase</keyword>
    <keyword evidence="20 29" id="KW-0829">Tyrosine-protein kinase</keyword>
    <keyword evidence="16" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="SH3" evidence="32">
        <location>
            <begin position="67"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="31">
        <location>
            <begin position="133"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="33">
        <location>
            <begin position="251"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="30">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="30">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="RefSeq" id="XP_017350896.1"/>
        </source>
    </evidence>
    <sequence length="516" mass="58832" checksum="29545EDC7DEA0A26" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T9A0</accession>
    <name>A0A2D0T9A0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13 14 15">Muscleblind-like protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13 14 15 16" type="primary">LOC108280598</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13 14 15" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13 14 15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the muscleblind family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017351229.1">
        <property type="nucleotide sequence ID" value="XM_017495740.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017351230.1">
        <property type="nucleotide sequence ID" value="XM_017495741.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017351231.1">
        <property type="nucleotide sequence ID" value="XM_017495742.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017351233.1">
        <property type="nucleotide sequence ID" value="XM_017495744.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T9A0"/>
    <dbReference type="GeneID" id="108280598"/>
    <dbReference type="KEGG" id="ipu:108280598"/>
    <dbReference type="OMA" id="ATSKVPM"/>
    <dbReference type="OrthoDB" id="6285980at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.210:FF:000004">
        <property type="entry name" value="Muscleblind like splicing regulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.210:FF:000002">
        <property type="entry name" value="Muscleblind-like 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.210">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041367">
        <property type="entry name" value="Znf-CCCH_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054429">
        <property type="entry name" value="Znf-CCCH_Muscleblind-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12675">
        <property type="entry name" value="MUSCLEBLIND-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12675:SF7">
        <property type="entry name" value="MUSCLEBLIND-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22628">
        <property type="entry name" value="zf-CCCH_10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14608">
        <property type="entry name" value="zf-CCCH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18044">
        <property type="entry name" value="zf-CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="13"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="47"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="178"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="214"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="13"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="47"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="178"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="214"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017351229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017351230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017351231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017351233.1"/>
        </source>
    </evidence>
    <sequence length="408" mass="44438" checksum="DF77A0513BE149E9" modified="2017-12-20" version="1">MAVNVTPIRDTKWLTLEVCREFQRGTCSRPDSECKFAHPAKSCQVENGRVIACFDSLKGRCSRENCKYLHPPPHLKTQLEINGRNNLIQQKNMAMLAQQMQLANAMMPGTQLQPVPMFSVTPSLATNATALVNPYLGPVSPGLVPTEILPSAPVLMTSTPNVPVPAAAAAAAQKLMRTDRLEVCREYQRGNCTRGENDCRFAHPSDSAMIDTNDNTVTVCMDYIKGRCSRDKCKYFHPPAHLQAKIKAAQHQVNQAAAAAAMTQSAVKSLKRPLEATFDLGIPPVLPPLPKRPALEKTNGATAMFNTGMFQYQQALTNMQFHQQAAFIPSGSILCMTPAASVVPMMHGASAATVSAATTSATSVPFATATANQRTNLPVLYSIWEKNNMIFYEPTNPKDSNNICRSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EZ41</accession>
    <name>A0A979EZ41_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">AP-1 complex-associated regulatory protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">ap1ar</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3 4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047013085.1">
        <property type="nucleotide sequence ID" value="XM_047157129.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047013086.1">
        <property type="nucleotide sequence ID" value="XM_047157130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EZ41"/>
    <dbReference type="GeneID" id="108269278"/>
    <dbReference type="CTD" id="55435"/>
    <dbReference type="OrthoDB" id="10069720at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035650">
        <property type="term" value="F:AP-1 adaptor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000146">
        <property type="term" value="P:negative regulation of cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900025">
        <property type="term" value="P:negative regulation of substrate adhesion-dependent cell spreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034315">
        <property type="term" value="P:regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048203">
        <property type="term" value="P:vesicle targeting, trans-Golgi to endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031483">
        <property type="entry name" value="AP1AR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34529">
        <property type="entry name" value="AP-1 COMPLEX-ASSOCIATED REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34529:SF1">
        <property type="entry name" value="AP-1 COMPLEX-ASSOCIATED REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15745">
        <property type="entry name" value="AP1AR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_047013085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_047013086.1"/>
        </source>
    </evidence>
    <sequence length="296" mass="33176" checksum="6A62C516F6052A1C" modified="2023-02-22" version="1">MGNCWTQCTGIFWREANRIQRGGGSKYFRSSTTGEHYTIEFENLVESDEGESSQSCARPISEEEIGHLKELRYAAIADTQTLIDEKLNAELVAQEEKLRLEEEAIVAAQREAARLAKERKLKEQQLAQKKTEKEGSSSHRKQPSGGEFEAYLQSVKAQSEAFRSSRLSSETNLVTPNTESSWDFTCRTRSTNDDATSLDLEWEDEEGMNRTGATWERSKTEEDILRAALRPAGKQLGSGPTSASEDSNALEWENDFVSAHAEDSSEQCDDDEFEGFVNPVLDTPSEAEPNADQQDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R5M8</accession>
    <name>A0A9F7R5M8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Teneurin-3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Protein Odd Oz/ten-m homolog 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Tenascin-M3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Teneurin transmembrane protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">tenm3</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Axon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the tenascin family. Teneurin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053535881.1">
        <property type="nucleotide sequence ID" value="XM_053679906.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108263307"/>
    <dbReference type="KEGG" id="ipu:108263307"/>
    <dbReference type="CTD" id="55714"/>
    <dbReference type="OrthoDB" id="442731at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000016">
        <property type="entry name" value="Teneurin transmembrane protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000026">
        <property type="entry name" value="Teneurin transmembrane protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000003">
        <property type="entry name" value="Teneurin transmembrane protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.180.10.10:FF:000004">
        <property type="entry name" value="Teneurin transmembrane protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000013">
        <property type="entry name" value="Teneurin transmembrane protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000005">
        <property type="entry name" value="Teneurin transmembrane protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000008">
        <property type="entry name" value="teneurin-3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.180.10.10">
        <property type="entry name" value="RHS repeat-associated core"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057627">
        <property type="entry name" value="FN-plug_TEN1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH_b-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022385">
        <property type="entry name" value="Rhs_assc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056823">
        <property type="entry name" value="TEN-like_YD-shell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009471">
        <property type="entry name" value="Ten_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056822">
        <property type="entry name" value="TEN_NHL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056820">
        <property type="entry name" value="TEN_TTR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051216">
        <property type="entry name" value="Teneurin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057629">
        <property type="entry name" value="Teneurin1-4_GBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028916">
        <property type="entry name" value="Tox-GHH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006530">
        <property type="entry name" value="YD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03696">
        <property type="entry name" value="Rhs_assc_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01643">
        <property type="entry name" value="YD_repeat_2x"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219">
        <property type="entry name" value="TENEURIN AND N-ACETYLGLUCOSAMINE-1-PHOSPHODIESTER ALPHA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219:SF65">
        <property type="entry name" value="TENEURIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25024">
        <property type="entry name" value="EGF_TEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24329">
        <property type="entry name" value="FN-plug_TEN1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23093">
        <property type="entry name" value="GBD_Tenm3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06484">
        <property type="entry name" value="Ten_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25021">
        <property type="entry name" value="TEN_NHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25023">
        <property type="entry name" value="TEN_YD-shell"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23538">
        <property type="entry name" value="Teneurin_ABD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15636">
        <property type="entry name" value="Tox-GHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25020">
        <property type="entry name" value="TTR_TEN1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="Soluble quinoprotein glucose dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51361">
        <property type="entry name" value="TENEURIN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12 19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 19" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Teneurin N-terminal" evidence="22">
        <location>
            <begin position="1"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="640"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="54"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="644"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="665"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00077040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_053535881.1"/>
        </source>
    </evidence>
    <sequence length="2608" mass="290094" checksum="D7B5345591886682" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R692</accession>
    <name>A0A9F7R692_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Fumarylacetoacetate hydrolase domain-containing protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC128630369</name>
        <name evidence="5" type="synonym">LOC128629602</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5 6">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053534317.1">
        <property type="nucleotide sequence ID" value="XM_053678342.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534810.1">
        <property type="nucleotide sequence ID" value="XM_053678835.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R692"/>
    <dbReference type="GeneID" id="128630369"/>
    <dbReference type="KEGG" id="ipu:128629602"/>
    <dbReference type="KEGG" id="ipu:128630369"/>
    <dbReference type="OrthoDB" id="411064at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR051121">
        <property type="entry name" value="FAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42796:SF4">
        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42796">
        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE DOMAIN-CONTAINING PROTEIN 2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="3">
        <location>
            <begin position="60"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053534317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_053534810.1"/>
        </source>
    </evidence>
    <sequence length="202" mass="22842" checksum="5FF061D24B831144" modified="2023-06-28" version="1">MERLQRVLHYCHSSSAKTNEANRRHETYPGYVWCEDKNHAKLEIHSYKKEASYAHACYSQEEDARGHVAGFTVASEISARVWQMKNGKHWLLGKTFDKFCPLEPALVTTEALKDVHNLGIRCLVNGDVVQDSNTSQLIFKTEKVVVWVSQFVTLYPGDVFLTGTPPGVGVFRKPPIFLKVSSVSSFLHCVGLFSIFQFCESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R6Z3</accession>
    <name>A0A9F7R6Z3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Polyadenylate-binding protein</fullName>
            <shortName evidence="7">PABP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">pabpc1b</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds the poly(A) tail of mRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the polyadenylate-binding protein type-1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053531238.1">
        <property type="nucleotide sequence ID" value="XM_053675263.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R6Z3"/>
    <dbReference type="GeneID" id="108257311"/>
    <dbReference type="CTD" id="393856"/>
    <dbReference type="OrthoDB" id="19742at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12378">
        <property type="entry name" value="RRM1_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12379">
        <property type="entry name" value="RRM2_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12380">
        <property type="entry name" value="RRM3_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12381">
        <property type="entry name" value="RRM4_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1900.10:FF:000001">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000021">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000042">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000154">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.10">
        <property type="entry name" value="c-terminal domain of poly(a) binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036053">
        <property type="entry name" value="PABP-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006515">
        <property type="entry name" value="PABP_1234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034364">
        <property type="entry name" value="PABP_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045305">
        <property type="entry name" value="RRM2_I_PABPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01628">
        <property type="entry name" value="PABP-1234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00658">
        <property type="entry name" value="MLLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00517">
        <property type="entry name" value="PolyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63570">
        <property type="entry name" value="PABC (PABP) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="11"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="99"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="191"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="294"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="PABC" evidence="10">
        <location>
            <begin position="538"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="453"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="453"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053531238.1"/>
        </source>
    </evidence>
    <sequence length="633" mass="70982" checksum="841E3516BE7B319A" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R6Z6</accession>
    <name>A0A9F7R6Z6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="2">E3 ubiquitin-protein ligase UBR2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC128630033</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053534592.1">
        <property type="nucleotide sequence ID" value="XM_053678617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R6Z6"/>
    <dbReference type="GeneID" id="128630033"/>
    <dbReference type="KEGG" id="ipu:128630033"/>
    <dbReference type="OrthoDB" id="26387at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_053534592.1"/>
        </source>
    </evidence>
    <sequence length="94" mass="10852" checksum="B15DF16C1A88B843" modified="2023-06-28" version="1">MLQRVLHLIGMALLEEQQQLENIGDDDEVTFNFTLKISRPGEPPGNTTSILALLETLQCTLHLEVHKDMIRWILKVKLHLDYVFNLPYSLYACT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RC15</accession>
    <name>A0A9F7RC15_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Leucine-rich repeat-containing protein 71 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">lrrc71</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053540294.1">
        <property type="nucleotide sequence ID" value="XM_053684319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RC15"/>
    <dbReference type="GeneID" id="108272681"/>
    <dbReference type="CTD" id="149499"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053040">
        <property type="entry name" value="LRR-containing_protein_71"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46984">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46984:SF1">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053540294.1"/>
        </source>
    </evidence>
    <sequence length="500" mass="55234" checksum="9F1DFBB4DABE3A5A" modified="2023-06-28" version="1">MKKRMEKVSKEKGKEVEEEPSHTAEWAELNPSKCDGLLNIQDKCPVQSIDDYQCSGNLEVDFPELCALVGLREIPEVRPLTAAPPSSKRAMENIQDQIFSESCMTIWSPKPRLHIELENEDSLSVKRVRISGWRVDEAMARVLSKTLPSLGNLQSLEMWQIGLTDTILTSMKNIISMCSKLRTVVLEGTPIPENSYHILLGEDSMITHLSLRNNCIGEDGARLISLALSKTLQSLNMAFNSVGDAGAVYLAQGLRLNRTLLYLSLANNHIGDVGASHLAQVIGPFALTHEEIVERRRQLKRKDQLLISEDSECESALSIPSSSSLECNIKSATKRKDGTKKEEKLPTNQTVTTTGKKEDPKVAKKRTSDTKIPQGRGGKSDEKDKHPSVKEQETEEVVETQSPLLNPAIQSTGGKVIYPGNTTLLSLNLSGNKLTQQSLQMFLSSVACQGKRGLQYISLNRNSFPPDCEVFLKLQEIMSLRNPVNRTTSAQAEAEQGQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RL28</accession>
    <name>A0A9F7RL28_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rho GTPase-activating protein 39</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">arhgap46b</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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        <property type="nucleotide sequence ID" value="XM_053686312.1"/>
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    <dbReference type="GeneID" id="108275877"/>
    <dbReference type="CTD" id="568268"/>
    <dbReference type="OrthoDB" id="437889at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:GTPase activator activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04389">
        <property type="entry name" value="RhoGAP_KIAA1688"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rho GTPase activating protein 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000011">
        <property type="entry name" value="Rho GTPase-activating protein 39"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MyTH4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000857">
        <property type="entry name" value="MyTH4_dom"/>
    </dbReference>
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        <property type="entry name" value="MyTH4_dom_sf"/>
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    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
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        <property type="entry name" value="WW_dom"/>
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    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45876">
        <property type="entry name" value="FI04035P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45876:SF6">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 39-LIKE"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00784">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00139">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51016">
        <property type="entry name" value="MYTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="122"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="MyTH4" evidence="11">
        <location>
            <begin position="668"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="10">
        <location>
            <begin position="872"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="187"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
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            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="409"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="499"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="535"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="799"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="210"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="260"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="416"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="446"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="545"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
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            <end position="601"/>
        </location>
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        <location>
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    </feature>
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            <end position="811"/>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00070269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_053542287.1"/>
        </source>
    </evidence>
    <sequence length="1065" mass="119190" checksum="8C6C9AB449F80362" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RLI1</accession>
    <name>A0A9F7RLI1_ICTPU</name>
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        <submittedName>
            <fullName evidence="7">Beta-microseminoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC108276563</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta-microseminoprotein family.</text>
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    <dbReference type="RefSeq" id="XP_053543075.1">
        <property type="nucleotide sequence ID" value="XM_053687100.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RLI1"/>
    <dbReference type="GeneID" id="108276563"/>
    <dbReference type="KEGG" id="ipu:108276563"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PSP94"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05825">
        <property type="entry name" value="PSP94"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039907179" evidence="5">
        <location>
            <begin position="20"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053543075.1"/>
        </source>
    </evidence>
    <sequence length="102" mass="11507" checksum="3AFD63D3ABDC9F4F" modified="2023-06-28" version="1">MRSVFVSFVLLALIPVNHAACWSKIKPGVTNCWDDLDKKWHLVGSSWRTRECQTCNCEVDYMRCCDGWPSVSGGCTIEYDYKTCTYEVIVTDKSVPCTAVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RR21</accession>
    <name>A0A9F7RR21_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">5-hydroxytryptamine receptor 1E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC108255563</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053542021.1">
        <property type="nucleotide sequence ID" value="XM_053686046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RR21"/>
    <dbReference type="GeneID" id="108255563"/>
    <dbReference type="KEGG" id="ipu:108255563"/>
    <dbReference type="OrthoDB" id="5956310at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004938">
        <property type="term" value="F:alpha2-adrenergic receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071881">
        <property type="term" value="P:adenylate cyclase-inhibiting adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000375">
        <property type="entry name" value="5-hydroxytryptamine (serotonin) receptor 1Fa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002231">
        <property type="entry name" value="5HT_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF196">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR 1D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01101">
        <property type="entry name" value="5HTRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="75"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="194"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="54"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_053542021.1"/>
        </source>
    </evidence>
    <sequence length="350" mass="39731" checksum="65D601D5D592C5FB" modified="2023-06-28" version="1">MERYLVKSSEAPPLINYTHSNSSNQGAEFGVAVATERLAVASLLGLLTLSTAVMNAAVITAILTTKKLRLPANYLICSLAVTDFLVAVLVMPLSILYIATATWNLGRVVCEVWLSVDMTCCTCSILHLCVIALDRYWAITKAVEYTRKRTPRRAVTMVIVVWVASMFISAPPLFWRRRHDDVHECAIEHDQLGYSIYSTFGAFYIPMTLILILYYRIYTAAKSLYQKRGSSRHFSDSLNHFRVDLSNSDPGVELEKLNSPDERNQICSSRERKAARILGLILGAFILCWLPFFIKELLMALKVLEPSPHVSDFLTWLGYMNSLINPLLYTSFNEDFKQAFKRLLKRKEHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RTT8</accession>
    <name>A0A9F7RTT8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Trichohyalin isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC108279277</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053543956.1">
        <property type="nucleotide sequence ID" value="XM_053687981.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108279277"/>
    <dbReference type="OrthoDB" id="8779660at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="4">
        <location>
            <begin position="144"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="307"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="819"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="922"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1075"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1201"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1547"/>
            <end position="1634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1547"/>
            <end position="1565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1572"/>
            <end position="1583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1595"/>
            <end position="1619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_053543956.1"/>
        </source>
    </evidence>
    <sequence length="1665" mass="199705" checksum="634F0103D442ED48" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TD12</accession>
    <name>A0A9F7TD12_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Synaptotagmin-17</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Synaptotagmin XVII</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">syt17</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptotagmin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053532604.1">
        <property type="nucleotide sequence ID" value="XM_053676629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TD12"/>
    <dbReference type="GeneID" id="108280421"/>
    <dbReference type="KEGG" id="ipu:108280421"/>
    <dbReference type="CTD" id="51760"/>
    <dbReference type="OrthoDB" id="270970at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070382">
        <property type="term" value="C:exocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017156">
        <property type="term" value="P:calcium-ion regulated exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08390">
        <property type="entry name" value="C2A_Synaptotagmin-15-17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08410">
        <property type="entry name" value="C2B_Synaptotagmin-17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000053">
        <property type="entry name" value="synaptotagmin-17 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000064">
        <property type="entry name" value="synaptotagmin-17 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001565">
        <property type="entry name" value="Synaptotagmin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047897">
        <property type="entry name" value="Synaptotagmin-15/17_C2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014705">
        <property type="entry name" value="Syt17_C2B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF348">
        <property type="entry name" value="SYNAPTOTAGMIN-17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00399">
        <property type="entry name" value="SYNAPTOTAGMN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="9">
        <location>
            <begin position="203"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="9">
        <location>
            <begin position="334"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="74"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="96"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053532604.1"/>
        </source>
    </evidence>
    <sequence length="487" mass="54862" checksum="0E20E39B8D89A8D2" modified="2023-06-28" version="1">MRRRGGSEDRMAYTQLEPVNEGLLSTLTDLLLCRWSCRACWHWCWECSCCQTTEEEVEILGPFPAQTPSWLVNDCNDEKESDSHLGSRDQVHAAHGDAAGSSSPSCTSPSSRGSGSENSRSTFSLARQLSSLDSRRPSSPLVDTKPIEFWALSPRKEAVQPLRKPQPPSDDYFRKLEPRLYSSESGGDDVDSLSDEELLLRYQLGLLHFSTQYDLVHCQLIVRVIEARDLPPPGAGVAHSNPYVKMSLLPDHKNSRQTGVKRKTQNPVFEERFTFELPFLEAQRRTLLLSVLDFDKFSRHCVIGKVTLPLSEVDLVKGGHWWKALVPSSQNELELGELLLSLNYLPSAGRLNVDVIRAKQLLQTDMCQGSDPFVKVQLVAGLKLVKTKKTSCMRGTIDPYFNESFSFRVPHEELSEVSLVFTVYGHNMKSSNDFVGRIVIGQFSTGPAETTHWRRLLRSQRTPVEQWHSLRSRAECDRVSPASLEVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TLY0</accession>
    <name>A0A9F7TLY0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Tripartite motif-containing protein 16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC128633058</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053537631.1">
        <property type="nucleotide sequence ID" value="XM_053681656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TLY0"/>
    <dbReference type="GeneID" id="128633058"/>
    <dbReference type="KEGG" id="ipu:128633058"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16040">
        <property type="entry name" value="SPRY_PRY_SNTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF5">
        <property type="entry name" value="E3 UBIQUITIN_ISG15 LIGASE TRIM25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="15"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="9">
        <location>
            <begin position="146"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="10">
        <location>
            <begin position="361"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="259"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053537631.1"/>
        </source>
    </evidence>
    <sequence length="549" mass="62918" checksum="2DA5B305E141A06B" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TM99</accession>
    <name>A0A9F7TM99_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Nuclear pore complex protein Nup160</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">nup160</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053538114.1">
        <property type="nucleotide sequence ID" value="XM_053682139.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108268519"/>
    <dbReference type="KEGG" id="ipu:108268519"/>
    <dbReference type="CTD" id="23279"/>
    <dbReference type="OrthoDB" id="67716at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059141">
        <property type="entry name" value="Beta-prop_Nup120_160"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021717">
        <property type="entry name" value="Nucleoporin_Nup160"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056547">
        <property type="entry name" value="NUP160_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056536">
        <property type="entry name" value="TPR_NUP160_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056535">
        <property type="entry name" value="TPR_NUP160_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21286">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21286:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11715">
        <property type="entry name" value="Beta-prop_Nup120_160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23345">
        <property type="entry name" value="NUP160_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23354">
        <property type="entry name" value="TPR_NUP160_120_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23347">
        <property type="entry name" value="TPR_Nup160_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup120/160 beta-propeller" evidence="5">
        <location>
            <begin position="74"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="NUP160 helical" evidence="6">
        <location>
            <begin position="567"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="domain" description="NUP160 middle TPR" evidence="8">
        <location>
            <begin position="843"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="domain" description="NUP160 C-terminal TPR" evidence="7">
        <location>
            <begin position="1153"/>
            <end position="1404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1122"/>
            <end position="1144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF11715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053538114.1"/>
        </source>
    </evidence>
    <sequence length="1408" mass="158307" checksum="BF3F9F8C03F53345" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TP56</accession>
    <name>A0A9F7TP56_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Syntaxin-8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">stx8</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Vesicle trafficking protein that functions in the early secretory pathway, possibly by mediating retrograde transport from cis-Golgi membranes to the ER.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053540004.1">
        <property type="nucleotide sequence ID" value="XM_053684029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TP56"/>
    <dbReference type="GeneID" id="108272526"/>
    <dbReference type="KEGG" id="ipu:108272526"/>
    <dbReference type="CTD" id="9482"/>
    <dbReference type="OrthoDB" id="428895at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15852">
        <property type="entry name" value="SNARE_Syntaxin8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000036">
        <property type="entry name" value="Putative Syntaxin-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041875">
        <property type="entry name" value="Syntaxin-8_SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF124">
        <property type="entry name" value="SYNTAXIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="14">
        <location>
            <begin position="146"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="7"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00072662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_053540004.1"/>
        </source>
    </evidence>
    <sequence length="246" mass="27949" checksum="E82CEF60CB0C6CD9" modified="2023-06-28" version="1">MSQDLWLQNYDATCRLAQEIAENIQERNRQQRTGGNPAKLNMSLRASLQKLKQNITQLRETLARASSQRRIMQSEADRRQNLVDDLVTREKQLFSSFSRDPSAPEPSRSTLLTGGFGAEPAQNPWLVDESEETKGLTFREIKEQQQRIIQAQDEGLDVLADVISRQKRMGQEIGNELDEHNEIIDDITQLVDTTDSRIRNETRRVQLVETKSASCGLAVRFPAHMTPHVEVSLGKTLNPKLLLMVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TPG0</accession>
    <name>A0A9F7TPG0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">A-type potassium channel modulatory protein DPP6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Dipeptidyl aminopeptidase-like protein 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Dipeptidyl peptidase 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Dipeptidyl peptidase VI</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">dpp6a</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="14">Homodimer (in vitro). Interacts with KCND2. Identified in a complex with KCND2 and KCNIP2. Forms an octameric complex composed of four DPP6 subunits bound to the KCND2 tetramer. Interacts with KCND3; this interaction modulates the channel gating kinetics namely channel activation and inactivation kinetics and rate of recovery from inactivation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S9B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053540226.1">
        <property type="nucleotide sequence ID" value="XM_053684251.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TPG0"/>
    <dbReference type="GeneID" id="108279432"/>
    <dbReference type="KEGG" id="ipu:108279432"/>
    <dbReference type="CTD" id="100007104"/>
    <dbReference type="OrthoDB" id="16520at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF20">
        <property type="entry name" value="DIPEPTIDYL AMINOPEPTIDASE-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="18">
        <location>
            <begin position="147"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="17">
        <location>
            <begin position="594"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_053540226.1"/>
        </source>
    </evidence>
    <sequence length="807" mass="91901" checksum="281F10DCD7B2466B" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TR82</accession>
    <name>A0A9F7TR82_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zinc finger protein basonuclin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108275607</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053542341.1">
        <property type="nucleotide sequence ID" value="XM_053686366.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TR82"/>
    <dbReference type="GeneID" id="108275607"/>
    <dbReference type="KEGG" id="ipu:108275607"/>
    <dbReference type="OrthoDB" id="10070972at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040436">
        <property type="entry name" value="Disconnected-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15021:SF0">
        <property type="entry name" value="DISCO-RELATED, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15021">
        <property type="entry name" value="DISCONNECTED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="253"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053542341.1"/>
        </source>
    </evidence>
    <sequence length="310" mass="34134" checksum="776DB3797304870B" modified="2023-06-28" version="1">MITAPSDSTDTRAHTSNNNTADNFHFKPHTTITVGDSDSARQQQENPQDKNIENNKGELETTRGLSLSLSCVCVCVCVCVCVALSKLSVCSNQVEVVHSGVVCDLSALILFSTHAFPIRMKILLDRLFSVLTHQHVLDVLHTHGWTLHDYIRGYKLQDSSGKVLDRWLIMTPEDELVTLRQFLRFGETKSSAELMISQREQLPLPTNGSETTKPNGFFNTSHNLKMNGIIRGQRSPGSSHSENLSGGVEECSEPPSSLLLEFPSELCQLQQDPKTKPRENTESEAAIVTNPPLRGGGGGERKTPGNEKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2008-04-29" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>B1NQM9</accession>
    <name>B1NQM9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Hemopexin precursor</fullName>
            <ecNumber evidence="11">3.2.1.35</ecNumber>
        </submittedName>
        <submittedName>
            <fullName evidence="9">Warm temperature acclimation protein 65 kDa-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Wap65-1</name>
        <name evidence="11" type="synonym">hpxa</name>
        <name evidence="11" type="synonym">wap65-1</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11" key="1">
        <citation type="journal article" date="2008" name="Dev. Comp. Immunol." volume="32" first="693" last="705">
            <title>The two channel catfish intelectin genes exhibit highly differential patterns of tissue expression and regulation after infection with Edwardsiella ictaluri.</title>
            <authorList>
                <person name="Takano T."/>
                <person name="Sha Z."/>
                <person name="Peatman E."/>
                <person name="Terhune J."/>
                <person name="Liu H."/>
                <person name="Kucuktas H."/>
                <person name="Li P."/>
                <person name="Edholm E.S."/>
                <person name="Wilson M."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="18078992"/>
            <dbReference type="DOI" id="10.1016/j.dci.2007.10.008"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="9 11" key="2">
        <citation type="journal article" date="2008" name="Mol. Immunol." volume="45" first="1458" last="1469">
            <title>The warm temperature acclimation protein Wap65 as an immune response gene: its duplicates are differentially regulated by temperature and bacterial infections.</title>
            <authorList>
                <person name="Sha Z."/>
                <person name="Xu P."/>
                <person name="Takano T."/>
                <person name="Liu H."/>
                <person name="Terhune J."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="17920125"/>
            <dbReference type="DOI" id="10.1016/j.molimm.2007.08.012"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="10" key="4">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
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        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">SDA103</strain>
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    </reference>
    <reference evidence="8" key="4">
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        <source>
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        <source>
            <dbReference type="RefSeq" id="NP_001187382.1"/>
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    </evidence>
    <sequence length="290" mass="32327" checksum="15B7A827CB84DBC2" modified="2011-01-11" version="1">MDTVKKGEKDCEEETLQTAFKRLRVDAESSVAALRGSETLAPRAGTRVSTDGTKPKITSPKENWHGCSRKSSRGLTRNQRRRRSKSPILHPPKFTYCSSKMQFKHKSPTELEDTSTSLLVPAKKELLSSRAHSPIFGSMGYDSNITLESKISPLNRVATFGNENKSEGKDERTNISLEEAQAPAADFKSLCQLQESGVCQCTSWHGMEVYSFTGLRDVISECEMKLPSSSDAPQNTRTVNGSSVSTSPRSCSEQARVHVDDITIEDLSGYMEYYLYIPKKMSHMAEMMYT</sequence>
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    </organism>
    <reference evidence="10 12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
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                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the archaeal Rpo10/eukaryotic RPB10 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GU589069">
        <property type="protein sequence ID" value="ADO29021.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187712.1">
        <property type="nucleotide sequence ID" value="NM_001200783.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TFD5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000025757"/>
    <dbReference type="GeneID" id="100528513"/>
    <dbReference type="KEGG" id="ipu:100528513"/>
    <dbReference type="CTD" id="5441"/>
    <dbReference type="GeneTree" id="ENSGT00390000007087"/>
    <dbReference type="OMA" id="YCCRRMF"/>
    <dbReference type="OrthoDB" id="10258858at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000024">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00250">
        <property type="entry name" value="RNApol_arch_Rpo10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023580">
        <property type="entry name" value="RNA_pol_su_RPB10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020789">
        <property type="entry name" value="RNA_pol_suN_Zn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000268">
        <property type="entry name" value="RPABC5/Rpb10"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003089">
        <property type="entry name" value="PRK04016.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431:SF3">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01194">
        <property type="entry name" value="RNA_pol_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005653">
        <property type="entry name" value="RNA_pol_N/8_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46924">
        <property type="entry name" value="RNA polymerase subunit RPB10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01112">
        <property type="entry name" value="RNA_POL_N_8KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3 10 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADO29021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="NP_001187712.1"/>
        </source>
    </evidence>
    <sequence length="67" mass="7675" checksum="8099106667FE61DA" modified="2011-01-11" version="1">MIIPIRCFTCGKIVGNKWEAYLGLLQAEYTEGDALDALGLKRYCCRRMLLSHVDLIEKLLNYAPLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3TG18</accession>
    <name>E3TG18_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoserine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">O-phosphoserine phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SERB</name>
        <name evidence="18" type="synonym">psph</name>
        <name evidence="18" type="synonym">serb</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 18" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the last irreversible step in the biosynthesis of L-serine from carbohydrates, the dephosphorylation of O-phospho-L-serine to L-serine. L-serine can then be used in protein synthesis, to produce other amino acids, in nucleotide metabolism or in glutathione synthesis, or can be racemized to D-serine, a neuromodulator. May also act on O-phospho-D-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-D-serine + H2O = D-serine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24873"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35247"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58680"/>
            <dbReference type="EC" id="3.1.3.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:24874"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>O-phospho-L-serine + H2O = L-serine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21208"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57524"/>
            <dbReference type="EC" id="3.1.3.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:21209"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAD-like hydrolase superfamily. SerB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.3" evidence="4"/>
    <dbReference type="EMBL" id="GU589303">
        <property type="protein sequence ID" value="ADO29254.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001188090.1">
        <property type="nucleotide sequence ID" value="NM_001201161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TG18"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000011508"/>
    <dbReference type="GeneID" id="100528891"/>
    <dbReference type="KEGG" id="ipu:100528891"/>
    <dbReference type="CTD" id="5723"/>
    <dbReference type="OrthoDB" id="27226at2759"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00198"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036424">
        <property type="term" value="F:L-phosphoserine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04309">
        <property type="entry name" value="HAD_PSP_eu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.210:FF:000002">
        <property type="entry name" value="Phosphoserine phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000077">
        <property type="entry name" value="Phosphoserine phosphatase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000114">
        <property type="entry name" value="Phosphoserine phosphatase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.210">
        <property type="entry name" value="Phosphoserine phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050582">
        <property type="entry name" value="HAD-like_SerB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004469">
        <property type="entry name" value="PSP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01488">
        <property type="entry name" value="HAD-SF-IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00338">
        <property type="entry name" value="serB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344:SF2">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="active site" description="Nucleophile" evidence="15">
        <location>
            <position position="20"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR604469-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ADO29254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="NP_001188090.1"/>
        </source>
    </evidence>
    <sequence length="226" mass="24925" checksum="F0FFA8014C22D637" modified="2011-01-11" version="1">MITLAQTKETFRLAEAVCFDVDSTVIREEGIDELAKFCGVGDAVTEMTRKAMGGSVAFKTALTERPSIIRCSREQVNKLITDHPPQLTPGIKELVERLHQRSVKVFLISGGFRCIVEHVATQLGIPLDHVYANRLKFYFNGEYAGFDETQPTAESGGKGKVISLLKEKYGFKKIVMIGDGATDLEACPPASGFIGFGGNVVRQQVKEKSSWYVTSFGELLKELEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3TG97</accession>
    <name>E3TG97_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP synthase subunit g</fullName>
            <shortName evidence="10">ATPase subunit g</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ATP5L</name>
        <name evidence="13" type="synonym">atp5l</name>
        <name evidence="13" type="synonym">atp5mg</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ATPase g subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GU589383">
        <property type="protein sequence ID" value="ADO29333.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187940.1">
        <property type="nucleotide sequence ID" value="NM_001201011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TG97"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012698"/>
    <dbReference type="GeneID" id="100528741"/>
    <dbReference type="KEGG" id="ipu:100528741"/>
    <dbReference type="CTD" id="326977"/>
    <dbReference type="GeneTree" id="ENSGT01030000234890"/>
    <dbReference type="OMA" id="KSGAWKN"/>
    <dbReference type="OrthoDB" id="437at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016702">
        <property type="entry name" value="ATP5MG_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006808">
        <property type="entry name" value="ATP_synth_F0_gsu_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12386">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04718">
        <property type="entry name" value="ATP-synt_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017835">
        <property type="entry name" value="ATP-synth_g_mitoch_animal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9 10" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4 10" id="KW-0138">CF(0)</keyword>
    <keyword evidence="5 10" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR017835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADO29333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="NP_001187940.1"/>
        </source>
    </evidence>
    <sequence length="103" mass="10986" checksum="6B31BB8163CA18D8" modified="2011-01-11" version="1">MAQAVQKLVAKVPSLVGAAVSYSKPRLSTFWYYARVELVPPSPAEISKAVAGVSDLAKAFQAGRLGQTTVRDALRNGLVATEVLMWFYIGEIIGKGGIVGYDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5U856</accession>
    <name>W5U856_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Synaptosomal-associated protein 47</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Synaptosomal-associated 47 kDa protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">snap47</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the SVAP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT407820">
        <property type="protein sequence ID" value="AHH38133.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017327879.1">
        <property type="nucleotide sequence ID" value="XM_017472390.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U856"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009010"/>
    <dbReference type="GeneID" id="108267894"/>
    <dbReference type="KEGG" id="ipu:108267894"/>
    <dbReference type="CTD" id="116841"/>
    <dbReference type="OMA" id="DICIHTW"/>
    <dbReference type="OrthoDB" id="10009801at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031629">
        <property type="term" value="P:synaptic vesicle fusion to presynaptic active zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016082">
        <property type="term" value="P:synaptic vesicle priming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000269">
        <property type="entry name" value="Synaptosomal-associated protein 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305">
        <property type="entry name" value="SYNAPTOSOMAL ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305:SF1">
        <property type="entry name" value="SYNAPTOSOMAL-ASSOCIATED PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="6">
        <location>
            <begin position="363"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AHH38133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017327879.1"/>
        </source>
    </evidence>
    <sequence length="418" mass="46392" checksum="795258072CD21E56" modified="2014-04-16" version="1">MDGEALVHSWPCSYYVNGDKRWVSGRLTLTPSAVRFSSSSGVTGGLLLPLSRVTHINTESSCFIFSAVTLQERDAHKHWFSSLKPSASAVFHVLQHFWRESLAVRTHSTHTPLSRGQRLISMVSDAQHTLTHTGRALSQQGEQFHHMIHQIDKMESDLGVADKLLSELESPAWWPFGKLPWNRKCQLELGKAEATAPPPEGAARSSKEILSVPVVFYRGGDINAADVNSGALVLLVSALEVFNANGRLIHRFHKQEVDEIRVHGVCDISVRQRHIGKPDVCFRLLSAKMAEALCVLEMQYKKKVEFARDYTGFQATPSDDITPGSAAFWGAGASSDSDVPVQVPAGDLTPVQLHVQQKTVTQEEAQELKQMLLQLKSLALEADSELQRQDEDLDVLMSSSDRSTATINTHTRRMRKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5U8N3</accession>
    <name>W5U8N3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5 7">Copine-7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Cpne7</name>
        <name evidence="7" type="synonym">cpne7</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the copine family.</text>
    </comment>
    <dbReference type="EMBL" id="JT408411">
        <property type="protein sequence ID" value="AHH38336.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017333593.1">
        <property type="nucleotide sequence ID" value="XM_017478104.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U8N3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000014259"/>
    <dbReference type="GeneID" id="108270990"/>
    <dbReference type="KEGG" id="ipu:108270990"/>
    <dbReference type="CTD" id="27132"/>
    <dbReference type="OMA" id="CSIGTRD"/>
    <dbReference type="OrthoDB" id="5855668at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04048">
        <property type="entry name" value="C2A_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04047">
        <property type="entry name" value="C2B_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000132">
        <property type="entry name" value="Copine 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000163">
        <property type="entry name" value="Copine 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037768">
        <property type="entry name" value="C2B_Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF6">
        <property type="entry name" value="COPINE-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="137"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="4">
        <location>
            <begin position="307"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AHH38336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017333593.1"/>
        </source>
    </evidence>
    <sequence length="558" mass="62387" checksum="E90BD3AA7FC59B81" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5UCP4</accession>
    <name>W5UCP4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EKC/KEOPS complex subunit TPRKB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">EKC/KEOPS complex subunit Tprkb</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">PRPK-binding protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">TP53RK-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Tprkb</name>
        <name evidence="14" type="synonym">tprkb</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the CGI121/TPRKB family.</text>
    </comment>
    <dbReference type="EMBL" id="JT411184">
        <property type="protein sequence ID" value="AHH39334.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017350048.1">
        <property type="nucleotide sequence ID" value="XM_017494559.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UCP4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000014293"/>
    <dbReference type="GeneID" id="108279921"/>
    <dbReference type="KEGG" id="ipu:108279921"/>
    <dbReference type="CTD" id="51002"/>
    <dbReference type="GeneTree" id="ENSGT00390000012942"/>
    <dbReference type="OMA" id="IVCRMST"/>
    <dbReference type="OrthoDB" id="329139at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2380.10:FF:000001">
        <property type="entry name" value="EKC/KEOPS complex subunit TPRKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2380.10">
        <property type="entry name" value="CGI121/TPRKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013926">
        <property type="entry name" value="CGI121/TPRKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036504">
        <property type="entry name" value="CGI121/TPRKB_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011465">
        <property type="entry name" value="PRK14886.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15840">
        <property type="entry name" value="CGI-121 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15840:SF10">
        <property type="entry name" value="EKC_KEOPS COMPLEX SUBUNIT TPRKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08617">
        <property type="entry name" value="CGI-121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143870">
        <property type="entry name" value="PF0523-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00075578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AHH39334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017350048.1"/>
        </source>
    </evidence>
    <sequence length="175" mass="19414" checksum="335B8A06E4CA30C2" modified="2014-04-16" version="1">MHLSNSLELFPEFVVTQLLFKDVKNCAELRKMAMEGKIAGALINPSVVLDPFQTLLAANKAVHVQKIGKMKTRSLYSEIIFNLSPTNNISEAFKRFGVSDSDNAVLIVLVHNKEEAYNKDDVLSKVDGQQIPADQMSTLSDLAKIKKLYKVAPQEEKCGNVLDAVICRMAAKDFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5UCV4</accession>
    <name>W5UCV4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Vacuolar protein-sorting-associated protein 36</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">ESCRT-II complex subunit VPS36</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12 14" type="primary">vps36</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the endosomal sorting complex required for transport II (ESCRT-II).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the VPS36 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT411385">
        <property type="protein sequence ID" value="AHH39409.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017345162.1">
        <property type="nucleotide sequence ID" value="XM_017489673.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UCV4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005406"/>
    <dbReference type="GeneID" id="108277181"/>
    <dbReference type="KEGG" id="ipu:108277181"/>
    <dbReference type="CTD" id="51028"/>
    <dbReference type="OMA" id="TLNARVW"/>
    <dbReference type="OrthoDB" id="271448at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000814">
        <property type="term" value="C:ESCRT II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13226">
        <property type="entry name" value="PH-GRAM-like_Eap45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000203">
        <property type="entry name" value="Vacuolar protein sorting 36 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000241">
        <property type="entry name" value="Vacuolar protein sorting 36 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000170">
        <property type="entry name" value="Vacuolar protein-sorting-associated protein 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021648">
        <property type="entry name" value="GLUE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040608">
        <property type="entry name" value="Snf8/Vps36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037855">
        <property type="entry name" value="Vps36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13128">
        <property type="entry name" value="VACUOLAR PROTEIN-SORTING-ASSOCIATED PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13128:SF12">
        <property type="entry name" value="VACUOLAR PROTEIN-SORTING-ASSOCIATED PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04157">
        <property type="entry name" value="EAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11605">
        <property type="entry name" value="Vps36_ESCRT-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51495">
        <property type="entry name" value="GLUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0967">Endosome</keyword>
    <keyword evidence="6 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="GLUE N-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="159"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AHH39409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017345162.1"/>
        </source>
    </evidence>
    <sequence length="381" mass="43302" checksum="7215E27193F5204F" modified="2014-04-16" version="1">MDRFMWTNGLLEMNETLVIHQRGVRLYDGDDKAKLDVGVVLLSTHRLIWRDQKNHECCICIPLSQIIFFEEQAAGIGKSAKIVIHLHPAPANREPGPYQNSKFSYIKLSFKEHGQIEFYRRLTEEITQRRWENTPVSQPITPAGLKTGRTRAVGIVGIERKLEEKRKETDKNISEAFEDLSKLMEKAKEMVELSRSIANKIKDKQGDITEDETIRFKSYLLSMGIANPVTRETHGSGTHYHMQLAKQLGDILLAPLEERGGMMALTEVYCLVNRARGMELLSPEDMVNACKLFESLKLPLRLRVFDSGVMVVQLQSHSEEEMITSAIDNVSEKGSLTAEEFAKLLGLSVLLAKERLLLAEKMGHLCRDDSVEGLRFYPNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5UEN2</accession>
    <name>W5UEN2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">AP complex subunit sigma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Ap2s1</name>
        <name evidence="15" type="synonym">ap2s1</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1). Interacts with CCDC32; the interaction is direct and mediates association of CCDC32 with adaptor protein complex 2 (AP-2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <location evidence="2">Coated pit</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="JT412883">
        <property type="protein sequence ID" value="AHH40034.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017346359.1">
        <property type="nucleotide sequence ID" value="XM_017490870.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UEN2"/>
    <dbReference type="GeneID" id="108277870"/>
    <dbReference type="KEGG" id="ipu:108277870"/>
    <dbReference type="CTD" id="1175"/>
    <dbReference type="OMA" id="QSNFVEY"/>
    <dbReference type="OrthoDB" id="371463at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030122">
        <property type="term" value="C:AP-2 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14833">
        <property type="entry name" value="AP2_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000004">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027156">
        <property type="entry name" value="APS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000804">
        <property type="entry name" value="Clathrin_sm-chain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015588">
        <property type="entry name" value="AP_complex_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00989">
        <property type="entry name" value="CLAT_ADAPTOR_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0168">Coated pit</keyword>
    <keyword evidence="6" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="12">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR015588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AHH40034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017346359.1"/>
        </source>
    </evidence>
    <sequence length="142" mass="16991" checksum="54CDD86ED5AF0BE5" modified="2014-04-16" version="1">MIRFILIQNRAGKTRLAKWYMQFDDDEKQKLIEEVHAVVTVRDAKHTNFVEFRNFKIIYRRYAGLYFCICVDVTDNNLAYLEGIHNFVEVLNEYFHNVCELDLVFNFYKVYTVVDEMFLAGEIRETSQTKVLKQLLMLQSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5UF77</accession>
    <name>W5UF77_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16 18">Kin of IRRE-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">KIRREL</name>
        <name evidence="18" type="synonym">kirrel1a</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="JT414473">
        <property type="protein sequence ID" value="AHH40633.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017327654.1">
        <property type="nucleotide sequence ID" value="XM_017472165.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UF77"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027776"/>
    <dbReference type="GeneID" id="108267762"/>
    <dbReference type="CTD" id="405804"/>
    <dbReference type="OrthoDB" id="10048737at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05898">
        <property type="entry name" value="IgI_5_KIRREL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000077">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000094">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000103">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051275">
        <property type="entry name" value="Cell_adhesion_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640:SF165">
        <property type="entry name" value="KIN OF IRRE LIKE (DROSOPHILA)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640">
        <property type="entry name" value="NEPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540268" evidence="14">
        <location>
            <begin position="21"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="498"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="21"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="120"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="222"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="308"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="392"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="594"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="783"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="632"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AHH40633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017327654.1"/>
        </source>
    </evidence>
    <sequence length="802" mass="86651" checksum="0A34E6CFF0511FFD" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5UFA2</accession>
    <name>W5UFA2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Beta-crystallin B1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Beta-B1 crystallin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CRYBB1</name>
        <name evidence="12" type="synonym">crybb1</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homo/heterodimer, or complexes of higher-order. The structure of beta-crystallin oligomers seems to be stabilized through interactions between the N-terminal arms.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="EMBL" id="JT414344">
        <property type="protein sequence ID" value="AHH40590.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017343896.1">
        <property type="nucleotide sequence ID" value="XM_017488407.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UFA2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018852"/>
    <dbReference type="GeneID" id="108276608"/>
    <dbReference type="KEGG" id="ipu:108276608"/>
    <dbReference type="CTD" id="1414"/>
    <dbReference type="GeneTree" id="ENSGT00940000160516"/>
    <dbReference type="OrthoDB" id="5411518at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000005">
        <property type="entry name" value="Crystallin, beta B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000002">
        <property type="entry name" value="Crystallin, beta B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF12">
        <property type="entry name" value="BETA-CRYSTALLIN B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="9">
        <location>
            <begin position="79"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="9">
        <location>
            <begin position="129"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="9">
        <location>
            <begin position="171"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AHH40590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017343896.1"/>
        </source>
    </evidence>
    <sequence length="230" mass="26564" checksum="5754C6B489A4F4C2" modified="2014-04-16" version="1">MSQTKSASSQGTDAKDKGAPAPPASSKTTKTGDAGMGNYRMMLFDQENFQGRMMEIYNECMNICDHGIDRVKSIIVECGPFVAFEQTNFRGEMFILEKGEYPRWDTWSNSYRSDCLMSFRPIRMDPMEHKICLYELSDFQGNKMEIHEDDVPTLWAHGFCDRVGSVRVPGGAWVGYQYPGYRGYQYLFECGDYRHYNEFCAFQPQIQSIRRVRDMQFHQRGSFSLTAAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5UML0</accession>
    <name>W5UML0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Tropomyosin alpha-3 chain</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="12">Tropomyosin alpha-4 chain isoform X3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Tpm3</name>
        <name evidence="12" type="synonym">LOC108280389</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the tropomyosin family.</text>
    </comment>
    <dbReference type="EMBL" id="JT419113">
        <property type="protein sequence ID" value="AHH42960.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017350797.1">
        <property type="nucleotide sequence ID" value="XM_017495308.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UML0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005442"/>
    <dbReference type="GeneID" id="108280389"/>
    <dbReference type="KEGG" id="ipu:108280389"/>
    <dbReference type="OMA" id="HAEAKCA"/>
    <dbReference type="OrthoDB" id="128924at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000001">
        <property type="entry name" value="Tropomyosin alpha-1 chain isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000004">
        <property type="entry name" value="tropomyosin alpha-1 chain isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000533">
        <property type="entry name" value="Tropomyosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19269">
        <property type="entry name" value="TROPOMYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00261">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00194">
        <property type="entry name" value="TROPOMYOSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00326">
        <property type="entry name" value="TROPOMYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="7"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AHH42960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017350797.1"/>
        </source>
    </evidence>
    <sequence length="248" mass="28678" checksum="0B09FFAC2D4855A6" modified="2014-04-16" version="1">MAGVNSVEAVKRKIKVLQQQADEAEERAEVLQRQVEEEKRSREQAEADVASLNRRIQLVEEELDRAQERLTTALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEVQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAEVAEAKSAELEEELRNVTNTLKSLEAQTEKYSQKEDKYEEEIKILTDKLKEAETRAEFGERSVTKLEKTIDDLEERLATAKEENLKIHATLDATLQDLNNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5UQU8</accession>
    <name>W5UQU8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Golgi SNAP receptor complex member 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">27 kDa Golgi SNARE protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Membrin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">Gosr2</name>
        <name evidence="21" type="synonym">gosr2</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 17">Involved in transport of proteins from the cis/medial-Golgi to the trans-Golgi network.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Golgi apparatus</location>
            <location evidence="12">cis-Golgi network membrane</location>
            <topology evidence="12">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 17">Belongs to the GOSR2 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT416126">
        <property type="protein sequence ID" value="AHH41938.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017314679.1">
        <property type="nucleotide sequence ID" value="XM_017459190.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UQU8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022740"/>
    <dbReference type="GeneID" id="108259586"/>
    <dbReference type="KEGG" id="ipu:108259586"/>
    <dbReference type="CTD" id="9570"/>
    <dbReference type="OMA" id="LKYDSRH"/>
    <dbReference type="OrthoDB" id="158360at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15863">
        <property type="entry name" value="SNARE_GS27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000044">
        <property type="entry name" value="Golgi SNAP receptor complex member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027027">
        <property type="entry name" value="GOSR2/Membrin/Bos1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230:SF1">
        <property type="entry name" value="GOLGI SNAP RECEPTOR COMPLEX MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230">
        <property type="entry name" value="VESICLE TRANSPORT V-SNARE PROTEIN VTI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12352">
        <property type="entry name" value="V-SNARE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028865">
        <property type="entry name" value="Membrin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 17 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 17" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19 21" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 17" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00076985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00080151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR028865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AHH41938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017314679.1"/>
        </source>
    </evidence>
    <sequence length="212" mass="24564" checksum="F0030C7180C6F318" modified="2014-04-16" version="1">METLYHQTKHQIEEIQSYMGRLKTTERQSVHLVENELQARIEQVFTHLERLMILAGKEPPNRRHNAKQRVEQLKYDVQHLQSALRNFQQGRYTREAQEKEREELMSRTFTTNDADTSIPIDDTLQFNSSLHSAHRGMDDLLGSGSSILNGLRDQRSTLKGTHKKMLDVANMLGLSNTVMRLIEKRASQDKFIMVGGMLITCVFMFLVVKYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PJ19</accession>
    <name>A0A2D0PJ19_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Trans-2,3-enoyl-CoA reductase-like</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Steroid 5-alpha-reductase 2-like 2 protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">tecrl2a</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the steroid 5-alpha reductase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017306568.2">
        <property type="nucleotide sequence ID" value="XM_017451079.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PJ19"/>
    <dbReference type="GeneID" id="108255190"/>
    <dbReference type="KEGG" id="ipu:108255190"/>
    <dbReference type="CTD" id="798606"/>
    <dbReference type="OrthoDB" id="540503at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000131">
        <property type="entry name" value="trans-2,3-enoyl-CoA reductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039357">
        <property type="entry name" value="SRD5A/TECR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049127">
        <property type="entry name" value="TECR-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556:SF59">
        <property type="entry name" value="STEROID 5-ALPHA REDUCTASE C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21696">
        <property type="entry name" value="TECR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="256"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="TECR-like N-terminal" evidence="13">
        <location>
            <begin position="6"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="12">
        <location>
            <begin position="159"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF21696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017306568.2"/>
        </source>
    </evidence>
    <sequence length="312" mass="36258" checksum="02E938A1CD060C54" modified="2023-02-22" version="2">MSVLLQVEILDDKTKEQLCFLDKQVEYHSTIGDIKGLFHRVYPHWYPARQALKLHPKSGELRDDELLQNLPVGLTATLYFRDLGPRFGWTMVFLLEYVGPLLIYLLFYFRLPYIYTHPHTLTPPGPDPAVSLACVCHSLHYIKRLMETVFVHRFTHGTLPLGVIIRNCVYYWGFAAWLAYYINHPLYTPPSYGRPQIGCALVMFTLCEVGNFSIHWSLNTSGGDAGDKYRSFPHPSKNPFTWLFFFVSCPNYTYEVGVWVSFSIMTQCVPVAVFTLIGFVQMTIWAKWKHKAYTREFKDYPTLRMSILPFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PJS2</accession>
    <name>A0A2D0PJS2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Protein Mdm4 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">mdm4</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the MDM2/MDM4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017306209.1">
        <property type="nucleotide sequence ID" value="XM_017450720.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PJS2"/>
    <dbReference type="GeneID" id="108255070"/>
    <dbReference type="KEGG" id="ipu:108255070"/>
    <dbReference type="CTD" id="4194"/>
    <dbReference type="OrthoDB" id="24526at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17673">
        <property type="entry name" value="MDM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16784">
        <property type="entry name" value="mRING-HC-C2H2C4_MDM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.245.10">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051652">
        <property type="entry name" value="MDM2_MDM4_MUL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016495">
        <property type="entry name" value="p53_neg-reg_MDM_2/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036885">
        <property type="entry name" value="SWIB_MDM2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003121">
        <property type="entry name" value="SWIB_MDM2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12183">
        <property type="entry name" value="MITOCHONDRIAL UBIQUITIN LIGASE ACTIVATOR OF NFKB 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12183:SF37">
        <property type="entry name" value="PROTEIN MDM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02201">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006748">
        <property type="entry name" value="p53_MDM_2/4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47592">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51925">
        <property type="entry name" value="SWIB_MDM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DM2" evidence="11">
        <location>
            <begin position="24"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="10">
        <location>
            <begin position="305"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="117"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="251"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="121"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR006748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017306209.1"/>
        </source>
    </evidence>
    <sequence length="473" mass="52897" checksum="D98BB468DBC6F057" modified="2017-12-20" version="1">MNSMSSALHSASSPSCRALPGEGSQVQPKAPLLQILRVAGAQEEIFTLKEVMHYLGQYIIVKELYDKQRQHIVHCQDDPLGELLEVESFSVKSPSPVYEMLKRNLLILNGSDAAKNVSVGKDSNQSPCEDPGQVSSKMCSSGTSFSDASACSMQNSSQRRPRDEDDDSLDGMPRSACKRPKLDVSLDEWDLSGLPWWFLGNLRSNYTRRSNGSTDIHTNQLFCGQEEDTAVVSDTTDDLWFLNEAESEQVSVEMKEAALEQGSDSESPQEEEEQGRDSKEDKEMQEELDDDSQCLSDDTDTEISSQDAWQCSECRKFNSATQRYCMRCWALRKDWYKDCTRLAHSVSVPNLPDPKDEDEDDGIDVPDCPRTVSDPVILPSHWASRDLPALPASAKGKAPRRVLQSSQESQEGREVMLEPCRLCRVRPRDGNIIHGRTAHMLTCFPCARKLHRCHAPCPGCGQIIQKVIKMFVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PN39</accession>
    <name>A0A2D0PN39_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17 18" type="primary">araf</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17 18">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017307429.1">
        <property type="nucleotide sequence ID" value="XM_017451940.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017307430.1">
        <property type="nucleotide sequence ID" value="XM_017451941.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PN39"/>
    <dbReference type="GeneID" id="108255753"/>
    <dbReference type="KEGG" id="ipu:108255753"/>
    <dbReference type="CTD" id="369"/>
    <dbReference type="OrthoDB" id="774951at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20870">
        <property type="entry name" value="C1_A_C-Raf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17133">
        <property type="entry name" value="RBD_ARAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000015">
        <property type="entry name" value="B-Raf proto-oncogene serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000024">
        <property type="entry name" value="B-Raf proto-oncogene serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000004">
        <property type="entry name" value="B-Raf proto-oncogene serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000036">
        <property type="entry name" value="RAF proto-oncogene serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003116">
        <property type="entry name" value="RBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF55">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE A-RAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02196">
        <property type="entry name" value="RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00455">
        <property type="entry name" value="RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50898">
        <property type="entry name" value="RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 17 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RBD" evidence="15">
        <location>
            <begin position="23"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="14">
        <location>
            <begin position="102"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="308"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="241"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="275"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017307429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017307430.1"/>
        </source>
    </evidence>
    <sequence length="606" mass="68047" checksum="436C11F6709900DF" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PQJ1</accession>
    <name>A0A2D0PQJ1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA (uracil(54)-C(5))-methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">trmt2a</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12 13">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>uridine(54) in tRNA + S-adenosyl-L-methionine = 5-methyluridine(54) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42712"/>
            <dbReference type="Rhea" id="RHEA-COMP:10167"/>
            <dbReference type="Rhea" id="RHEA-COMP:10193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74447"/>
            <dbReference type="EC" id="2.1.1.35"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:42713"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.35" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017307354.1">
        <property type="nucleotide sequence ID" value="XM_017451865.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017307355.1">
        <property type="nucleotide sequence ID" value="XM_017451866.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PQJ1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000024945"/>
    <dbReference type="GeneID" id="108255710"/>
    <dbReference type="KEGG" id="ipu:108255710"/>
    <dbReference type="CTD" id="27037"/>
    <dbReference type="GeneTree" id="ENSGT00530000063723"/>
    <dbReference type="OMA" id="TPLWNMP"/>
    <dbReference type="OrthoDB" id="10250660at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030697">
        <property type="term" value="F:tRNA (uracil(54)-C5)-methyltransferase activity, S-adenosyl methionine-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12439">
        <property type="entry name" value="RRM_TRMT2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030390">
        <property type="entry name" value="MeTrfase_TrmA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
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        <property type="entry name" value="TRNA (URACIL-5-)-METHYLTRANSFERASE HOMOLOG A"/>
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        <property type="entry name" value="tRNA_U5-meth_tr"/>
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        <location>
            <position position="550"/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PS00</accession>
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        <submittedName>
            <fullName evidence="11">Catenin delta-2 isoform X1</fullName>
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        <name type="scientific">Ictalurus punctatus</name>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        </subcellularLocation>
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        <text evidence="2">Belongs to the beta-catenin family.</text>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Plakophilin/d_Catenin"/>
    </dbReference>
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        <property type="entry name" value="CATENIN DELTA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10372">
        <property type="entry name" value="PLAKOPHILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Arm"/>
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    </dbReference>
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        <property type="entry name" value="ARM"/>
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    </dbReference>
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        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM_REPEAT"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
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            <end position="25"/>
        </location>
    </feature>
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        </location>
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        <location>
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        </location>
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        <location>
            <begin position="214"/>
            <end position="230"/>
        </location>
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        <location>
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        <location>
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        </location>
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        <location>
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            <end position="1003"/>
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        <location>
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        </location>
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        <location>
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        </location>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005462"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017308802.1"/>
        </source>
    </evidence>
    <sequence length="1183" mass="128511" checksum="403C3BEB1D7B7EBC" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PTA8</accession>
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        <recommendedName>
            <fullName evidence="13">Thioredoxin domain-containing protein 5</fullName>
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            <fullName evidence="15">Endoplasmic reticulum resident protein 46</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Thioredoxin-like protein p46</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">txndc5</name>
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    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Protein disulfide isomerase of the endoplasmic reticulum lumen involved in the formation of disulfide bonds in proteins. Can reduce insulin disulfide bonds.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017308330.1">
        <property type="nucleotide sequence ID" value="XM_017452841.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PTA8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026972"/>
    <dbReference type="GeneID" id="108256213"/>
    <dbReference type="KEGG" id="ipu:108256213"/>
    <dbReference type="CTD" id="81567"/>
    <dbReference type="OMA" id="TKHQTLC"/>
    <dbReference type="OrthoDB" id="71336at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03005">
        <property type="entry name" value="PDI_a_ERp46"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000146">
        <property type="entry name" value="Thioredoxin domain containing 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000164">
        <property type="entry name" value="Thioredoxin domain containing 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000147">
        <property type="entry name" value="Thioredoxin domain-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051063">
        <property type="entry name" value="PDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005788">
        <property type="entry name" value="PDI_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01126">
        <property type="entry name" value="pdi_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE C17H9.14C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672:SF3">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012903669" description="Thioredoxin domain-containing protein 5" evidence="17">
        <location>
            <begin position="21"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="18">
        <location>
            <begin position="6"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="18">
        <location>
            <begin position="141"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="18">
        <location>
            <begin position="268"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00079986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00080189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017308330.1"/>
        </source>
    </evidence>
    <sequence length="403" mass="45776" checksum="8C4FCB772350C8C7" modified="2017-12-20" version="1">MHRVLISVLCSAFLFTVILCDNETDQDEHIKHTYTVEMFNNAISTAPHFVMFFAPWCGHCQRLQGTWNELADKYNSMETPPVYVVKVDCTQDTKFCSSNHGIRGYPTLKLFKPEQEAVKYQGPRDLHSLETWMLKTLQEETVAPQSEPEAPKVPEALQGMYELTASNFKEHVSKGAHFVKFFAPWCGHCKAMAPTWEQLAISFEHSDLVKISKLDCTQHYEICSENQVRGYPTLLFFTDGEKVDQYRGKRDLESFKEFVDNQLAAASLKVKAAAEDAKGNEIPQNTDSVKDESTVKILTESNFAESTAKGISFIKFYAPWCGHCKNLAPVWEDLSKKDFPGLTDVKIAKVDCTVERTLCNRFSVNGYPTLLIFRGGQLVEEYNSGRDLESLHSFVMKQARDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PUX8</accession>
    <name>A0A2D0PUX8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 3 subunit H</fullName>
            <shortName evidence="5">eIF3h</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Eukaryotic translation initiation factor 3 subunit 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">eIF-3 gamma</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">eIF3 p40 subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">eif3hb</name>
        <name evidence="5" type="synonym">EIF3H</name>
        <name evidence="5" type="synonym">EIF3S3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eIF-3 subunit H family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017310338.1">
        <property type="nucleotide sequence ID" value="XM_017454849.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PUX8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021252"/>
    <dbReference type="GeneID" id="108257247"/>
    <dbReference type="KEGG" id="ipu:108257247"/>
    <dbReference type="CTD" id="567151"/>
    <dbReference type="OMA" id="RLETMCA"/>
    <dbReference type="OrthoDB" id="10265695at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08065">
        <property type="entry name" value="MPN_eIF3h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000020">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03007">
        <property type="entry name" value="eIF3h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027524">
        <property type="entry name" value="eIF3h"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045810">
        <property type="entry name" value="eIF3h_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050242">
        <property type="entry name" value="JAMM_MPN+_peptidase_M67A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10410">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19445">
        <property type="entry name" value="eIF3h_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5 9" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPN" evidence="7">
        <location>
            <begin position="20"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="252"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="252"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00057041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017310338.1"/>
        </source>
    </evidence>
    <sequence length="333" mass="38157" checksum="36251FBE18AF4FC6" modified="2017-12-20" version="1">MAARKDASPGTSLESAVKQVQIEGLVVLKIIKHYQEEGQGSEVVQGVLLGLVVDDRLEITNCFPFPQHTEDDADFDEVQYQMEMMRSLRHVNIDHLHVGWYQSTFFGSFMSRALLDSQFSYQHAIEESVVLIYDPIKTSQGSLSLKAYRLTPKLMEICKEKDFSAEGLKKASVGYENMFEEVPIVIKNSHLINVLLWELEDKSTVVEKHELLSLSSSSHLEKSLQMLMDRVDDMCQDINKYNNFSRSLSKQQQQKHQYVQRRQQENAQRQSRGEPPLPEEDLTKLFKPPQPPPRMDSLLIAGQINTYCQTVKEFTSQNLGKLFMAEALQDHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PYG4</accession>
    <name>A0A2D0PYG4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">NACHT, LRR and PYD domains-containing protein 3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC108257668</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017311077.1">
        <property type="nucleotide sequence ID" value="XM_017455588.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PYG4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000023233"/>
    <dbReference type="GeneID" id="108257668"/>
    <dbReference type="KEGG" id="ipu:108257668"/>
    <dbReference type="OMA" id="QTINLFH"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000100">
        <property type="entry name" value="Si:dkey-11n14.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000210">
        <property type="entry name" value="Si:dkey-16p6.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="18"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="57"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017311077.1"/>
        </source>
    </evidence>
    <sequence length="989" mass="111831" checksum="CBC3DCADAB967D86" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q2Y3</accession>
    <name>A0A2D0Q2Y3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Proto-oncogene vav</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC108258736</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017313088.1">
        <property type="nucleotide sequence ID" value="XM_017457599.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q2Y3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000016661"/>
    <dbReference type="GeneID" id="108258736"/>
    <dbReference type="KEGG" id="ipu:108258736"/>
    <dbReference type="OMA" id="PYISRPT"/>
    <dbReference type="OrthoDB" id="5340910at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21262">
        <property type="entry name" value="CH_VAV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01223">
        <property type="entry name" value="PH_Vav"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000009">
        <property type="entry name" value="Vav guanine nucleotide exchange factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000015">
        <property type="entry name" value="Vav guanine nucleotide exchange factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000019">
        <property type="entry name" value="Vav guanine nucleotide exchange factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022613">
        <property type="entry name" value="CH_CAMSAP_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037832">
        <property type="entry name" value="PH_Vav"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003096">
        <property type="entry name" value="SM22_calponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45818">
        <property type="entry name" value="PROTEIN VAV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45818:SF2">
        <property type="entry name" value="PROTO-ONCOGENE VAV"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C1_1"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF11971">
        <property type="entry name" value="CAMSAP_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00888">
        <property type="entry name" value="SM22CALPONIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00741">
        <property type="entry name" value="DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 10" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 11" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="16">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="15">
        <location>
            <begin position="193"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="14">
        <location>
            <begin position="401"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="17">
        <location>
            <begin position="515"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="589"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="12">
        <location>
            <begin position="668"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="783"/>
            <end position="843"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017313088.1"/>
        </source>
    </evidence>
    <sequence length="846" mass="97879" checksum="7B8A67F3C367A5D6" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q5N9</accession>
    <name>A0A2D0Q5N9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Probable ribonuclease ZC3H12C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC108259022</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZC3H12 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017458135.3"/>
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    <dbReference type="KEGG" id="ipu:108259022"/>
    <dbReference type="OrthoDB" id="392925at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036464">
        <property type="term" value="C:cytoplasmic ribonucleoprotein granule"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18729">
        <property type="entry name" value="PIN_Zc3h12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11980:FF:000001">
        <property type="entry name" value="ZC3H12A isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040546">
        <property type="entry name" value="Rege-1_UBA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040757">
        <property type="entry name" value="Regnase_1/ZC3H12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021869">
        <property type="entry name" value="RNase_Zc3h12_NYN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051101">
        <property type="entry name" value="ZC3H12/N4BP1_RNase_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12876">
        <property type="entry name" value="N4BP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12876:SF36">
        <property type="entry name" value="RIBONUCLEASE ZC3H12C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18561">
        <property type="entry name" value="Regnase_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11977">
        <property type="entry name" value="RNase_Zc3h12a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18039">
        <property type="entry name" value="UBA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="39"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="168"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="417"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="584"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="662"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="703"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="786"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="174"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="430"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="454"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="482"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="584"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017313624.1"/>
        </source>
    </evidence>
    <sequence length="862" mass="95316" checksum="7B02A109EC581058" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q7E5</accession>
    <name>A0A2D0Q7E5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">U1 small nuclear ribonucleoprotein 70 kDa</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">snrnp70</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017314222.1">
        <property type="nucleotide sequence ID" value="XM_017458733.3"/>
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    <dbReference type="STRING" id="7998.ENSIPUP00000020652"/>
    <dbReference type="GeneID" id="108259335"/>
    <dbReference type="KEGG" id="ipu:108259335"/>
    <dbReference type="CTD" id="6625"/>
    <dbReference type="OMA" id="GRTTKGW"/>
    <dbReference type="OrthoDB" id="4207594at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:U1 snRNP"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="RRM_snRNP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000153">
        <property type="entry name" value="U1 small nuclear ribonucleoprotein 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
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        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034143">
        <property type="entry name" value="snRNP70_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051183">
        <property type="entry name" value="U1_U11-U12_snRNP_70-35kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022023">
        <property type="entry name" value="U1snRNP70_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952:SF5">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12220">
        <property type="entry name" value="U1snRNP70_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="103"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="48"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="186"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="60"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="207"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="257"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="285"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="298"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="312"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="328"/>
            <end position="337"/>
        </location>
    </feature>
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        <location>
            <begin position="338"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="353"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="380"/>
            <end position="448"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017314222.1"/>
        </source>
    </evidence>
    <sequence length="513" mass="59407" checksum="51F79F28BA838C6C" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q7U1</accession>
    <name>A0A2D0Q7U1_ICTPU</name>
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        <recommendedName>
            <fullName evidence="4">Heat shock cognate 71 kDa protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC108259092</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the heat shock protein 70 family.</text>
    </comment>
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    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10233">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000025">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000001">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000028">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000135">
        <property type="entry name" value="Heat shock cognate 71 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000134">
        <property type="entry name" value="Heat shock cognate 71 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000026">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="620"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017313781.2"/>
        </source>
    </evidence>
    <sequence length="643" mass="70727" checksum="F3FB036DCCF92BD7" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q930</accession>
    <name>A0A2D0Q930_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zinc transporter ZIP13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Solute carrier family 39 member 13</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Zrt- and Irt-like protein 13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">slc39a13</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Zn(2+)(in) = Zn(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29351"/>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017314802.1">
        <property type="nucleotide sequence ID" value="XM_017459313.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q930"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027150"/>
    <dbReference type="GeneID" id="108259661"/>
    <dbReference type="KEGG" id="ipu:108259661"/>
    <dbReference type="CTD" id="91252"/>
    <dbReference type="OMA" id="HEVPHHI"/>
    <dbReference type="OrthoDB" id="200954at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16950">
        <property type="entry name" value="ZINC TRANSPORTER SLC39A7 HISTIDINE-RICH MEMBRANE PROTEIN KE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16950:SF16">
        <property type="entry name" value="ZINC TRANSPORTER ZIP13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0864">Zinc transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="363"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="395"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="253"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017314802.1"/>
        </source>
    </evidence>
    <sequence length="417" mass="44036" checksum="BC94E0A21058EBE5" modified="2017-12-20" version="1">MAGTSRRRHFSILGKTPFCIAAPCMLIRSTLRKSGLMSAGPGRPIWALAAVLVGATLLAVTSTSSSSEKKVAQTALAHATAAAAGPGTWCAEEMLGFHSLTKLLSGEGVDVWFCSLVGSIAVGLSGIFPLLVIPIEAGAALKTEAGCQNLKKLLSFAIGGLLGDVFLHLLPEAWANTSSNNQREKHYRTQGLWVVMGLLSFLLLEKMFPDEDSASESSAINRANNSSSADHQCDYSTSSEVNGVCSNNNSNSKPTTDISSQPGREKIKTSGYLNLLANCIDNFTHGLAVAGSFLVSRKVGFLTTFAILLHEIPHEVGDFAILLRAGFDRWSAARMQLSTALGGVLGACFALSAQSQQGAENAAAWILPFSSGGFLYIALVSVVPDLLEETNFRHSLLQILLIFCGVGVMALLSAIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QB90</accession>
    <name>A0A2D0QB90_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Acetylcholine receptor subunit epsilon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">chrne</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017315559.1">
        <property type="nucleotide sequence ID" value="XM_017460070.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QB90"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032938"/>
    <dbReference type="GeneID" id="108260097"/>
    <dbReference type="KEGG" id="ipu:108260097"/>
    <dbReference type="CTD" id="1145"/>
    <dbReference type="OrthoDB" id="5975154at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19029">
        <property type="entry name" value="LGIC_ECD_nAChR_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19064">
        <property type="entry name" value="LGIC_TM_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000079">
        <property type="entry name" value="Cholinergic receptor, nicotinic, epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000010">
        <property type="entry name" value="Nicotinic acetylcholine receptor subunit epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000012">
        <property type="entry name" value="Nicotinic acetylcholine receptor subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15 19" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 19" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="11 23" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 19" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022272721" evidence="19">
        <location>
            <begin position="25"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="245"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="311"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="477"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="20">
        <location>
            <begin position="29"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="21">
        <location>
            <begin position="251"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017315559.1"/>
        </source>
    </evidence>
    <sequence length="517" mass="59079" checksum="D8873A4262DE9BD0" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QDZ5</accession>
    <name>A0A2D0QDZ5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">APC membrane recruitment protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">amer1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
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    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Amer family.</text>
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    <dbReference type="RefSeq" id="XP_017315946.1">
        <property type="nucleotide sequence ID" value="XM_017460457.3"/>
    </dbReference>
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    <dbReference type="STRING" id="7998.ENSIPUP00000024852"/>
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    <dbReference type="OMA" id="WRDFPGT"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060828">
        <property type="term" value="P:regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019003">
        <property type="entry name" value="AMER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22237:SF0">
        <property type="entry name" value="APC MEMBRANE RECRUITMENT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22237">
        <property type="entry name" value="APC MEMBRANE RECRUITMENT PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09422">
        <property type="entry name" value="AMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0879">Wnt signaling pathway</keyword>
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            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="138"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="378"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="550"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="853"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="936"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="179"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="221"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="240"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="268"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="384"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="418"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="453"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="550"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="567"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="955"/>
            <end position="973"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017315946.1"/>
        </source>
    </evidence>
    <sequence length="973" mass="105622" checksum="FB479424B3A31535" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QF35</accession>
    <name>A0A2D0QF35_ICTPU</name>
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        <recommendedName>
            <fullName evidence="7">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">slc6a16a</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017316904.1">
        <property type="nucleotide sequence ID" value="XM_017461415.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QF35"/>
    <dbReference type="GeneID" id="108260811"/>
    <dbReference type="KEGG" id="ipu:108260811"/>
    <dbReference type="CTD" id="555229"/>
    <dbReference type="OrthoDB" id="6581954at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="6">Blood</tissue>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017318749.2"/>
        </source>
    </evidence>
    <sequence length="339" mass="38959" checksum="BE18A86D5AB44A8E" modified="2023-02-22" version="2">MGLEKASEKAKANTVGGRNKPKKIFGKTLPTGVVQSQDDSSVRVKQSKPDAHELLRESHISLLQLSAVEVATQLSVRAFELFHAIEPTEYIDDLFRLRSCLPRTSGLKLFYESINRETFWVATEVLREPNQLKRMEIIKHFIKIALYCRECKNFNSMFAIISGLNLAPVSRMRGTWKKLPSKYEKLFNDLQDLFDPSRNMAKYRNVLKSQNMQLPIIPLFPIFIKDLTFLHDGNDSMVDGLVNLEKLRMIAKEIRHVGRMAAVNVDAELLFRTRCLNRGRANADVLDVTQTRRHKKLYEDTQMAPKVKQYLSNLTVETNEKSLQTLSIQCEPSITRTFL</sequence>
</entry>
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    <accession>A0A2D0QKU1</accession>
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            <fullName evidence="4">Centrosomal protein of 44 kDa</fullName>
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        <name evidence="12" type="primary">cep44</name>
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        <name type="scientific">Ictalurus punctatus</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Centriole-enriched microtubule-binding protein involved in centriole biogenesis. In collaboration with CEP295 and POC1B, is required for the centriole-to-centrosome conversion by ensuring the formation of bona fide centriole wall. Functions as a linker component that maintains centrosome cohesion. Associates with CROCC and regulates its stability and localization to the centrosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle pole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Midbody</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017318864.1">
        <property type="nucleotide sequence ID" value="XM_017463375.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QKU1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000004403"/>
    <dbReference type="GeneID" id="108263006"/>
    <dbReference type="KEGG" id="ipu:108263006"/>
    <dbReference type="CTD" id="80817"/>
    <dbReference type="OrthoDB" id="259598at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
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                <person name="Yuan Z."/>
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        <property type="project" value="UniProtKB-KW"/>
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            <end position="42"/>
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    <sequence length="211" mass="22414" checksum="9D0E08B4C87FC03D" modified="2017-12-20" version="1">MAQETNQTQVPMLCTMGCGFYGNPRTNGMCSVCYKEHLQRQQAGGRSSPPGDKAASSPSGSPGTPAVTVETDPDPVSETAAPSEETSSSSGPVTQQMTAMSISQDTAATDSDRADDEEDEASSKSTGPAAEAAQASSDGDQTPDQNKKKNRCFTCRKKVGLTGFNCRCSNLFCAVHRYSDKHDCPYDYRGAAAAQLRKENPIVVAEKIQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R0J1</accession>
    <name>A0A2D0R0J1_ICTPU</name>
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        <submittedName>
            <fullName evidence="19 20">Peregrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19 20" type="primary">brpf1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="18">SDA103</strain>
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        <citation type="submission" date="2025-04" db="UniProtKB">
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="19 20">Blood</tissue>
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    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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        <property type="entry name" value="Znf_C2H2_type"/>
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        <property type="entry name" value="Znf_FYVE_PHD"/>
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        <property type="entry name" value="Znf_PHD"/>
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        <property type="entry name" value="Znf_PHD-finger"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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        <property type="entry name" value="PHD FINGER PROTEINS"/>
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        <property type="entry name" value="BROMODOMAIN"/>
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        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS01359">
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    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8 10" id="KW-0103">Bromodomain</keyword>
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    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="15">
        <location>
            <begin position="314"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="17">
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        <location>
            <begin position="692"/>
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            <begin position="1147"/>
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            <begin position="46"/>
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    <feature type="region of interest" description="Disordered" evidence="13">
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        <location>
            <begin position="175"/>
            <end position="190"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017324158.1"/>
        </source>
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        <source>
            <dbReference type="RefSeq" id="XP_017324160.1"/>
        </source>
    </evidence>
    <sequence length="1276" mass="142762" checksum="ED6C720C0F19B59D" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R1Z8</accession>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="14">SDA103</strain>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <source>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R486</accession>
    <name>A0A2D0R486_ICTPU</name>
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        <name evidence="13" type="primary">cdca7a</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Teleostei</taxon>
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            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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        <property type="component" value="Chromosome 6"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="Znf-4CXXC_R1"/>
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    <dbReference type="PANTHER" id="PTHR31169:SF2">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31169">
        <property type="entry name" value="OS05G0300700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10497">
        <property type="entry name" value="zf-4CXXC_R1"/>
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    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Zinc-finger" evidence="11">
        <location>
            <begin position="292"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="27"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="176"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="71"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF10497"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017324615.1"/>
        </source>
    </evidence>
    <sequence length="400" mass="45358" checksum="00BCF824FE237A5B" modified="2017-12-20" version="1">MRSQRQQAPPPSTRMNLRSYRNLGLVPMETSSSEDSCDSFASDGFASMKRPLRQTRSSARQEKLLETQSVSEEEGCSGFEESDCSNEMSAMKMDSDSDSEPPKKTRRSFTLRVAMKYPTKNVPQKKSPSPEPAPKAKGRQLDAEEEDFIAKRALNIKENKAMLAKLMAELNKVPGLFPKKTSLPASDTPRRVPRRSLGTPGTRRQNPERLSRIHTRSRSLVDGPPTPTPEEEPEDRFSLVRRCDDDDEEPEPRRRRTYNGTLTIPHVVRPVEEITRAELDNICTNVREKVYNRATGSTCHQCRQKTTDTKTNCRNPECVGVRGQFCGPCLRNRYGEEVHDALLNPEWQCPPCRGICNCSFCRAREGRCATGVLVYLAKYHGFDNVHAYLNSLRKELEESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R4X6</accession>
    <name>A0A2D0R4X6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Follistatin-related protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Follistatin-like protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">fstl1b</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Secreted glycoprotein that is involved in various physiological processes, such as angiogenesis, regulation of the immune response, cell proliferation and differentiation. Plays a role in the development of the central nervous system, skeletal system, lungs, and ureter. Promotes endothelial cell survival, migration and differentiation into network structures in an AKT-dependent manner. Also promotes survival of cardiac myocytes. Initiates various signaling cascades by activating different receptors on the cell surface such as DIP2A, TLR4 or BMP receptors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer. Interacts with SCN10A. Interacts with DIP2A; DIP2A may act as a cell surface receptor for FSTL1. Interacts with BMP4. Interacts with CD14; this interaction promotes TL4-mediated signaling cascade.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017470164.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R4X6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035652"/>
    <dbReference type="GeneID" id="108266613"/>
    <dbReference type="KEGG" id="ipu:108266613"/>
    <dbReference type="CTD" id="324309"/>
    <dbReference type="OrthoDB" id="88467at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030510">
        <property type="term" value="P:regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16233">
        <property type="entry name" value="EFh_SPARC_FSTL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.30:FF:000017">
        <property type="entry name" value="Follistatin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Follistatin-like 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
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        <property type="entry name" value="EF-hand_FSTL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003645">
        <property type="entry name" value="Fol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015369">
        <property type="entry name" value="Follistatin/Osteonectin_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050653">
        <property type="entry name" value="Prot_Inhib_GrowthFact_Antg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10913">
        <property type="entry name" value="FOLLISTATIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10913:SF13">
        <property type="entry name" value="FOLLISTATIN-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23564">
        <property type="entry name" value="EF-hand_FSTL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09289">
        <property type="entry name" value="FOLN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23244">
        <property type="entry name" value="VWF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00274">
        <property type="entry name" value="FOLN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039954205" description="Follistatin-related protein 1" evidence="13">
        <location>
            <begin position="19"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="15">
        <location>
            <begin position="50"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="190"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017325653.2"/>
        </source>
    </evidence>
    <sequence length="309" mass="34721" checksum="54F9972A28DAB8F5" modified="2023-06-28" version="2">MFRSLAAVVLLAAVCCHAQETQSKSKVCANVFCGAGRECAVTEKGEPSCLCIEQCKPHKRSVCGSNGKTYRNHCELHRDACLTGLKIQVAHDGHCKERKTEKAAASPVVCYVGDRNELRRRVIEWLQTEVVPDGWFTKGSNFTDLLLKYFKNYDNGDSQLDSAELLRFIQHNESAVELHSYAEDENNRLLRSLCVDALIELSDQNADWKLSFDEFLNCLRPGFNPPEKKCALEDETYEDGAETQVDCNLCVCACGNWVCTAMTCDEKTPALELATGDEPMTEEEWSLRVAELNKHQETMEQMKSSTKEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R5I2</accession>
    <name>A0A2D0R5I2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ETS domain-containing transcription factor ERF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">erfl3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017325070.1">
        <property type="nucleotide sequence ID" value="XM_017469581.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R5I2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008605"/>
    <dbReference type="GeneID" id="108266380"/>
    <dbReference type="KEGG" id="ipu:108266380"/>
    <dbReference type="CTD" id="565909"/>
    <dbReference type="OMA" id="RQSNGQM"/>
    <dbReference type="OrthoDB" id="8964711at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000059">
        <property type="entry name" value="ETS translocation variant 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF313">
        <property type="entry name" value="ETS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00345">
        <property type="entry name" value="ETS_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ETS" evidence="9">
        <location>
            <begin position="27"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="139"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="356"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="428"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="150"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="384"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="431"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="457"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="506"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="554"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017325070.1"/>
        </source>
    </evidence>
    <sequence length="612" mass="67022" checksum="6C9F90B0B9917284" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R7J9</accession>
    <name>A0A2D0R7J9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="15">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC108266671</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Golgi apparatus membrane</location>
            <topology evidence="2 15">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="15"/>
    <dbReference type="RefSeq" id="XP_017325795.1">
        <property type="nucleotide sequence ID" value="XM_017470306.3"/>
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    <dbReference type="STRING" id="7998.ENSIPUP00000008280"/>
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    <dbReference type="KEGG" id="ipu:108266671"/>
    <dbReference type="OrthoDB" id="9982049at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000088">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF130">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 15" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 15" id="KW-0430">Lectin</keyword>
    <keyword evidence="14 15" id="KW-0464">Manganese</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ricin B lectin" evidence="16">
        <location>
            <begin position="682"/>
            <end position="802"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017325795.1"/>
        </source>
    </evidence>
    <sequence length="807" mass="91306" checksum="BF0AB70C4934AB3B" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R7U6</accession>
    <name>A0A2D0R7U6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein LOC108267183 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC108267183</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017326662.1">
        <property type="nucleotide sequence ID" value="XM_017471173.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R7U6"/>
    <dbReference type="GeneID" id="108267183"/>
    <dbReference type="KEGG" id="ipu:108267183"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="5"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017326662.1"/>
        </source>
    </evidence>
    <sequence length="110" mass="12914" checksum="4ED68CF074CA9821" modified="2017-12-20" version="1">MENNYYIVVKTHKGEVKTIFVSNSEDEFNKTTILAFKEKILEAFSYTPEDQIRIICHGSQLEDTDTFAMRKIKNEDTFHLCLRLRGGQLQPDEESKDRRHSRDLTLSPDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R8M7</accession>
    <name>A0A2D0R8M7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Kelch-like protein 18</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">klhl18</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017326937.1">
        <property type="nucleotide sequence ID" value="XM_017471448.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R8M7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003387"/>
    <dbReference type="GeneID" id="108267404"/>
    <dbReference type="KEGG" id="ipu:108267404"/>
    <dbReference type="CTD" id="23276"/>
    <dbReference type="OMA" id="DRWTIVT"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="CDD" id="cd18457">
        <property type="entry name" value="BACK_KLHL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18247">
        <property type="entry name" value="BTB_POZ_KLHL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000001">
        <property type="entry name" value="Kelch-like family member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030603">
        <property type="entry name" value="KLHL18_BTB/POZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF497">
        <property type="entry name" value="KELCH-LIKE PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="40"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017326937.1"/>
        </source>
    </evidence>
    <sequence length="576" mass="64018" checksum="D54DA3EA6FCCAFFF" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RAN7</accession>
    <name>A0A2D0RAN7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13">P2Y purinoceptor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">si:dkey-6n21.13</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12 13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017327617.1">
        <property type="nucleotide sequence ID" value="XM_017472128.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017327618.1">
        <property type="nucleotide sequence ID" value="XM_017472129.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RAN7"/>
    <dbReference type="GeneID" id="108267748"/>
    <dbReference type="KEGG" id="ipu:108267748"/>
    <dbReference type="OrthoDB" id="10011262at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008142">
        <property type="term" value="F:oxysterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15968">
        <property type="entry name" value="7tmA_P2Y6_P2Y3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000609">
        <property type="entry name" value="Si:dkey-6n21.13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047160">
        <property type="entry name" value="GP183-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24237">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24237:SF36">
        <property type="entry name" value="P2Y PURINOCEPTOR 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="75"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="118"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="161"/>
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            <begin position="229"/>
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    </feature>
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        <location>
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            <begin position="57"/>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017327618.1"/>
        </source>
    </evidence>
    <sequence length="428" mass="48900" checksum="CC43BCC8B16C4511" modified="2017-12-20" version="1">MVTDTPTGLSETGQGGDTFFLFSSNSSSAASCNIDESYKYIFLPICYSFTFVFSISLNSVVLYRSFRRTKHWNVSLIYMVNLATTDFMYGLSLPFLVASYIMHDNWVFGDFMCRLVRFLFYFNLYCSIFFLTCISVHRYLGICHPMRTITLETKRAVKGTCILVWLAVFALTCPIFRFAQTELLSRRKDSGAIEVLEVDGDTQKSNISPNAEMFHNCWDDAIDKEFQDYVPYGITLHLLGFFVPFSIIAWCYSRVVMTIFRTLHSQTPANEPRTRQGSKAGARGEGMSIILGAHSPYVNRRRKSIKTIITITLLFALCFFPFHVTRTIFLVLKVTSRVQCHTMQMVSICYKITRPLASFNAWLNALLYFLTKEKSGPCCNKPPPSRHGLLWPLRMLGKGGNQEEEAEEAVNHKNNELKAENNIFTDLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RIE2</accession>
    <name>A0A2D0RIE2_ICTPU</name>
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        <submittedName>
            <fullName evidence="11">Uncharacterized protein LOC108269173 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC108269173</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
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    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Recycling endosome</location>
        </subcellularLocation>
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        <property type="nucleotide sequence ID" value="XM_017474810.3"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:small GTPase binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0045055">
        <property type="term" value="P:regulated exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="rab11 family-interacting protein 2 isoform X1"/>
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    </dbReference>
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        <property type="entry name" value="C2_domain_sf"/>
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        <property type="entry name" value="FIP-RBD_C_sf"/>
    </dbReference>
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        <property type="entry name" value="FIP_classI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019018">
        <property type="entry name" value="Rab-bd_FIP-RBD"/>
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        <property type="entry name" value="RAB11 FAMILY-INTERACTING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15746">
        <property type="entry name" value="RAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RBD-FIP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Eferin C-derminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FIP_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="C2" evidence="8">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="FIP-RBD" evidence="9">
        <location>
            <begin position="1312"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="442"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
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            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="974"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1083"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1119"/>
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        </location>
    </feature>
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        <location>
            <begin position="1217"/>
            <end position="1266"/>
        </location>
    </feature>
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        <location>
            <begin position="1316"/>
            <end position="1362"/>
        </location>
    </feature>
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        <location>
            <begin position="509"/>
            <end position="518"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        </location>
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        <location>
            <begin position="1152"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1217"/>
            <end position="1228"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017330299.1"/>
        </source>
    </evidence>
    <sequence length="1378" mass="150740" checksum="C5F29BC2DAC3FB32" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RMK9</accession>
    <name>A0A2D0RMK9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">SR-related and CTD-associated factor 8 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">scaf8</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017331386.1">
        <property type="nucleotide sequence ID" value="XM_017475897.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RMK9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000016050"/>
    <dbReference type="GeneID" id="108269826"/>
    <dbReference type="KEGG" id="ipu:108269826"/>
    <dbReference type="CTD" id="22828"/>
    <dbReference type="OrthoDB" id="79367at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="CID_SCAF8"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="CID_dom"/>
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        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034370">
        <property type="entry name" value="SCAF8_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051485">
        <property type="entry name" value="SR-CTD_assoc_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140">
        <property type="entry name" value="RNA PROCESSING PROTEIN LD23810P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140:SF1">
        <property type="entry name" value="SR-RELATED CTD ASSOCIATED FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CID" evidence="10">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="478"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="338"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="385"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="601"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="864"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="346"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="400"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="448"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="1014"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1054"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1126"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1152"/>
            <end position="1163"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
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            <end position="1205"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
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            <begin position="1262"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017331386.1"/>
        </source>
    </evidence>
    <sequence length="1271" mass="139199" checksum="56EE89AB35A9C329" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RPI9</accession>
    <name>A0A2D0RPI9_ICTPU</name>
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        <recommendedName>
            <fullName evidence="15">Alpha-1,4 glucan phosphorylase</fullName>
            <ecNumber evidence="15">2.4.1.1</ecNumber>
        </recommendedName>
    </protein>
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        <name evidence="17" type="primary">pygb</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Glycogen phosphorylase that regulates glycogen mobilization. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + phosphate = [(1-&gt;4)-alpha-D-glucosyl](n-1) + alpha-D-glucose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:41732"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9586"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 15">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer. Dimers associate into a tetramer to form the enzymatically active phosphorylase A.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the glycogen phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.1" evidence="15"/>
    <dbReference type="RefSeq" id="XP_017331870.2">
        <property type="nucleotide sequence ID" value="XM_017476381.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RPI9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020200"/>
    <dbReference type="GeneID" id="108270071"/>
    <dbReference type="KEGG" id="ipu:108270071"/>
    <dbReference type="CTD" id="5834"/>
    <dbReference type="OrthoDB" id="9215500at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008184">
        <property type="term" value="F:glycogen phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04300">
        <property type="entry name" value="GT35_Glycogen_Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000005">
        <property type="entry name" value="Alpha-1,4 glucan phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000153">
        <property type="entry name" value="Alpha-1,4 glucan phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000197">
        <property type="entry name" value="Alpha-1,4 glucan phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011833">
        <property type="entry name" value="Glycg_phsphrylas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000811">
        <property type="entry name" value="Glyco_trans_35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035090">
        <property type="entry name" value="Pyridoxal_P_attach_site"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02093">
        <property type="entry name" value="P_ylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468">
        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468:SF29">
        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE, BRAIN FORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00343">
        <property type="entry name" value="Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000460">
        <property type="entry name" value="Pprylas_GlgP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00102">
        <property type="entry name" value="PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="11 15" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="7 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9 14 15" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="14">
        <location>
            <position position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000460-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017331870.2"/>
        </source>
    </evidence>
    <sequence length="843" mass="97359" checksum="76F4EA31F9B506A4" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RR06</accession>
    <name>A0A2D0RR06_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Eukaryotic translation initiation factor 4 gamma 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">eif4g2a</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with the serine/threonine protein kinases MKNK1 and MKNK2. Binds EIF4A and EIF3. Interacts with MIF4GD. Interacts with DAZAP2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic initiation factor 4G family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017332929.1">
        <property type="nucleotide sequence ID" value="XM_017477440.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RR06"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000015080"/>
    <dbReference type="GeneID" id="108270613"/>
    <dbReference type="KEGG" id="ipu:108270613"/>
    <dbReference type="CTD" id="323453"/>
    <dbReference type="OrthoDB" id="514777at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11559">
        <property type="entry name" value="W2_eIF4G1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000007">
        <property type="entry name" value="Eukaryotic translation initiation factor 4 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000011">
        <property type="entry name" value="Eukaryotic translation initiation factor 4 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000017">
        <property type="entry name" value="Eukaryotic translation initiation factor 4 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003307">
        <property type="entry name" value="W2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23253">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4 GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23253:SF9">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4 GAMMA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02847">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02020">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00515">
        <property type="entry name" value="eIF5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00544">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51363">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 18" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="MI" evidence="16">
        <location>
            <begin position="592"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="domain" description="W2" evidence="15">
        <location>
            <begin position="769"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="77"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="539"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="119"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017332929.1"/>
        </source>
    </evidence>
    <sequence length="955" mass="108292" checksum="D4CE3C56B07668C1" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RSY1</accession>
    <name>A0A2D0RSY1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cilia- and flagella-associated protein 418</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cfap418</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May be involved in photoreceptor outer segment disk morphogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Photoreceptor inner segment</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017333276.1">
        <property type="nucleotide sequence ID" value="XM_017477787.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RSY1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000031087"/>
    <dbReference type="GeneID" id="108270830"/>
    <dbReference type="KEGG" id="ipu:108270830"/>
    <dbReference type="CTD" id="157657"/>
    <dbReference type="OrthoDB" id="259905at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001917">
        <property type="term" value="C:photoreceptor inner segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029239">
        <property type="entry name" value="CFAP418"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33958:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33958">
        <property type="entry name" value="PROTEIN C8ORF37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14996">
        <property type="entry name" value="RMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017333276.1"/>
        </source>
    </evidence>
    <sequence length="226" mass="25431" checksum="B60980FB5A77AEEE" modified="2017-12-20" version="1">MQDSKALFIHNSARERVAKVTSSLYEVSSALRALYTTLISRGGAHGIFLTFRAARKRKMRRLLPNRVSNPHEVEGVDNNIEAVLQEILNDDDYPASSSHRPVLSKPCAMESCSHTALKKCCPVYLGGSSTACGVGTSVTQKACDQIRCTSCDFRVVMFDDQEWDSSCDYLFFRNNMPDCNKLRVKLKRRQGARAYACQCSWHSAFMLSELRHVPKLKWICGQHTAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RTS0</accession>
    <name>A0A2D0RTS0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane channel-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 11 12" type="primary">tmc6b</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11 12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11 12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TMC family.</text>
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    <dbReference type="RefSeq" id="XP_017333894.1">
        <property type="nucleotide sequence ID" value="XM_017478405.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017333895.1">
        <property type="nucleotide sequence ID" value="XM_017478406.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017333897.1">
        <property type="nucleotide sequence ID" value="XM_017478408.3"/>
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    <dbReference type="STRING" id="7998.ENSIPUP00000031952"/>
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    <dbReference type="OrthoDB" id="1936208at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="TMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012496">
        <property type="entry name" value="TMC_dom"/>
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    <dbReference type="PANTHER" id="PTHR23302:SF4">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07810">
        <property type="entry name" value="TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
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            <end position="283"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="363"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="430"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="484"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="614"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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    </feature>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU310713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017333894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017333895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017333897.1"/>
        </source>
    </evidence>
    <sequence length="808" mass="92939" checksum="93E9C9D827BDB2B9" modified="2017-12-20" version="1"> </sequence>
</entry>
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        <recommendedName>
            <fullName evidence="3 6">26S proteasome non-ATPase regulatory subunit 1</fullName>
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        <name evidence="11" type="primary">psmd1</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
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            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
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    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
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                <person name="Liu H."/>
                <person name="Armbruster J."/>
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                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
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                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
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    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
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        <text evidence="1 6">Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.</text>
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        <text evidence="6">Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP).</text>
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        <text evidence="2 6">Belongs to the proteasome subunit S1 family.</text>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="26S_Psome_Rpn2"/>
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        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
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        <property type="entry name" value="Proteasome/cyclosome_rpt"/>
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    <dbReference type="InterPro" id="IPR048570">
        <property type="entry name" value="PSMD1_RPN2_N"/>
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        <property type="entry name" value="RPN2_C"/>
    </dbReference>
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        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943:SF2">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01851">
        <property type="entry name" value="PC_rep"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18004">
        <property type="entry name" value="RPN2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21505">
        <property type="entry name" value="RPN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015947">
        <property type="entry name" value="26S_Psome_Rpn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="26S proteasome non-ATPase regulatory subunit 1/RPN2 N-terminal" evidence="9">
        <location>
            <begin position="3"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="26S proteasome regulatory subunit RPN2 C-terminal" evidence="8">
        <location>
            <begin position="787"/>
            <end position="935"/>
        </location>
    </feature>
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        </location>
    </feature>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
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            <end position="965"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017333785.1"/>
        </source>
    </evidence>
    <sequence length="965" mass="107523" checksum="AB98B3F48677D875" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RVX1</accession>
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    <protein>
        <submittedName>
            <fullName evidence="21">Potassium voltage-gated channel subfamily A member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">kcna3a</name>
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    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017334648.1">
        <property type="nucleotide sequence ID" value="XM_017479159.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RVX1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034972"/>
    <dbReference type="GeneID" id="108271529"/>
    <dbReference type="KEGG" id="ipu:108271529"/>
    <dbReference type="CTD" id="100150068"/>
    <dbReference type="OMA" id="HECCERM"/>
    <dbReference type="OrthoDB" id="415460at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000002">
        <property type="entry name" value="Potassium voltage-gated channel subfamily a member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000007">
        <property type="entry name" value="Potassium voltage-gated channel subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000021">
        <property type="entry name" value="Potassium voltage-gated channel subfamily A member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003972">
        <property type="entry name" value="K_chnl_volt-dep_Kv1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF28">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY A MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01496">
        <property type="entry name" value="SHAKERCHANEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="7" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="350"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="411"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="19">
        <location>
            <begin position="64"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017334648.1"/>
        </source>
    </evidence>
    <sequence length="514" mass="58382" checksum="A68A6CE8732A3EF4" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RZ65</accession>
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        <submittedName>
            <fullName evidence="9">Plakophilin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pkp1b</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
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    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta-catenin family.</text>
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    <dbReference type="RefSeq" id="XP_017335250.2">
        <property type="nucleotide sequence ID" value="XM_017479761.3"/>
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    <dbReference type="STRING" id="7998.ENSIPUP00000037018"/>
    <dbReference type="GeneID" id="108271871"/>
    <dbReference type="KEGG" id="ipu:108271871"/>
    <dbReference type="CTD" id="797838"/>
    <dbReference type="OrthoDB" id="3245100at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028435">
        <property type="entry name" value="Plakophilin/d_Catenin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10372:SF3">
        <property type="entry name" value="PLAKOPHILIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10372">
        <property type="entry name" value="PLAKOPHILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="5" id="KW-0965">Cell junction</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="277"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="319"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="87"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="167"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="50"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="102"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="185"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017335250.2"/>
        </source>
    </evidence>
    <sequence length="717" mass="78389" checksum="E59AD3A408D6FD47" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RZ81</accession>
    <name>A0A2D0RZ81_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6 7">Eukaryotic translation initiation factor 4E transporter isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6 7 8" type="primary">eif4enif1</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5 6 7" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5 6 7">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017335263.1">
        <property type="nucleotide sequence ID" value="XM_017479774.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017335265.1">
        <property type="nucleotide sequence ID" value="XM_017479776.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017335266.1">
        <property type="nucleotide sequence ID" value="XM_017479777.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047014500.1">
        <property type="nucleotide sequence ID" value="XM_047158544.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RZ81"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026015"/>
    <dbReference type="GeneID" id="108271877"/>
    <dbReference type="KEGG" id="ipu:108271877"/>
    <dbReference type="CTD" id="56478"/>
    <dbReference type="GeneTree" id="ENSGT00390000012071"/>
    <dbReference type="OMA" id="QKGNGTP"/>
    <dbReference type="OrthoDB" id="8916892at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036464">
        <property type="term" value="C:cytoplasmic ribonucleoprotein granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018862">
        <property type="entry name" value="eIF4E-T"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12269">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12269:SF1">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10477">
        <property type="entry name" value="EIF4E-T"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6 7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 6 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="330"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="649"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="705"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="901"/>
            <end position="928"/>
        </location>
    </feature>
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        <location>
            <begin position="332"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="347"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="652"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="679"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="721"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="913"/>
            <end position="923"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017335263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017335265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017335266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_047014500.1"/>
        </source>
    </evidence>
    <sequence length="963" mass="106195" checksum="89729810084FC091" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RZX9</accession>
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        <submittedName>
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        <name evidence="2" type="primary">glmnb</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SDA103</strain>
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    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Blood</tissue>
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    <dbReference type="RefSeq" id="XP_017335741.1">
        <property type="nucleotide sequence ID" value="XM_017480252.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A2D0RZX9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000014862"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0055105">
        <property type="term" value="F:ubiquitin-protein transferase inhibitor activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="InterPro" id="IPR019516">
        <property type="entry name" value="Glomulin/ALF4"/>
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    <dbReference type="InterPro" id="IPR013877">
        <property type="entry name" value="YAP-bd/ALF4/Glomulin"/>
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    <dbReference type="PANTHER" id="PTHR15430">
        <property type="entry name" value="GLOMULIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR15430:SF1">
        <property type="entry name" value="GLOMULIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF08568">
        <property type="entry name" value="Kinetochor_Ybp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_017335741.1"/>
        </source>
    </evidence>
    <sequence length="591" mass="67473" checksum="B9635277C0A7A9DD" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S0C1</accession>
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        <recommendedName>
            <fullName evidence="4">MORN repeat-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC108272347</name>
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    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Assembles a suppression complex (suppresome) by tethering SIRT1 and MDM2 to regulate composite modifications of p53/TP53. Confers both deacetylation-mediated functional inactivation, by SIRT1, and ubiquitination-dependent degradation, by MDM2, of p53/TP53, promoting a proliferative and cell survival behaviors. May play a role in the regulation of spermatogenesis.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Acrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017336198.1">
        <property type="nucleotide sequence ID" value="XM_017480709.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S0C1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000010878"/>
    <dbReference type="GeneID" id="108272347"/>
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    <dbReference type="OrthoDB" id="270720at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
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    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052472">
        <property type="entry name" value="MORN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46511">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46511:SF1">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00045851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017336198.1"/>
        </source>
    </evidence>
    <sequence length="237" mass="27920" checksum="23F919642914E570" modified="2017-12-20" version="1">MALIGKPKIKESRWKVVEKKTQKDGLLHTFYSPNGDQYTGEWRKNLRHGQGTQVWTSAKTMYEGEWKWDVRDGFGTLYKLQLPSERYLKVYLGNWRNDKKEGFGKYFYSSSSRYEGEWVRNERSGNGRMTYENGDVYEGEWFRDKPHGKGVLLLKNGNRYEGYMKDGKKQGHGRFVYKDMGQVYEGFWVDDVPKCGKLCEYNRKNIHTPELNPIPPVRLQDVHEVLREALGRFCSMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SAE1</accession>
    <name>A0A2D0SAE1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Inward rectifier potassium channel 16 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">kcnj16a</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017339702.1">
        <property type="nucleotide sequence ID" value="XM_017484213.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SAE1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006128"/>
    <dbReference type="GeneID" id="108274207"/>
    <dbReference type="KEGG" id="ipu:108274207"/>
    <dbReference type="CTD" id="100001432"/>
    <dbReference type="OMA" id="CVFEVRS"/>
    <dbReference type="OrthoDB" id="273257at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005242">
        <property type="term" value="F:inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000019">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1400:FF:000003">
        <property type="entry name" value="Potassium voltage-gated channel subfamily J member 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1400">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041647">
        <property type="entry name" value="IRK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016449">
        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013518">
        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040445">
        <property type="entry name" value="Kir_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF24">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01007">
        <property type="entry name" value="IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17655">
        <property type="entry name" value="IRK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005465">
        <property type="entry name" value="GIRK_kir"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01320">
        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12 14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0630">Potassium</keyword>
    <keyword evidence="3 12" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 12" id="KW-0813">Transport</keyword>
    <keyword evidence="5 12" id="KW-0851">Voltage-gated channel</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017339702.1"/>
        </source>
    </evidence>
    <sequence length="377" mass="43085" checksum="ADCC163496E9BEE3" modified="2017-12-20" version="1">MPASSMNSRTVYYSNIWTDDGYCSTKRRYVHKDGSCKIVLLHVPEKWSLYVTDLFTTLVEIRWRVMFLTFALSYILSWLFFGILFWFIAVIHGDTRDANNEPCIYEVRSFTAAFLFSLETQTTIGYGSRGMSENCMVAIITVTIQDVISVFIDTFIIGIVVAKMASARKRAQTVGFSNTAVINCHNGHLCLSWRVGDFRRNHMVEGTTHAQLVRHSLHTSGKANITYQDLEIQNRHVILATPVIIIHQINRSSPLYKMSLQELRKDSFDLVVTFTYTDDRSGILHQSRTSYTPSEILWGQQFQEMVRVTQRSYRVDYAVFHQTVKVSLAENSAEDCELNKHAPSCKQEPQMGCSLAVESEDILGQNNTNTVIQEEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SB16</accession>
    <name>A0A2D0SB16_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Inactive rhomboid protein</fullName>
            <shortName evidence="11">iRhom</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Rhomboid family member</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Rhomboid veinlet-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">rhbdf1b</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Regulates ADAM17 protease, a sheddase of the epidermal growth factor (EGF) receptor ligands and TNF, thereby plays a role in sleep, cell survival, proliferation, migration and inflammation. Does not exhibit any protease activity on its own.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Endoplasmic reticulum membrane</location>
            <topology evidence="2 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017339922.1">
        <property type="nucleotide sequence ID" value="XM_017484433.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SB16"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000002707"/>
    <dbReference type="GeneID" id="108274327"/>
    <dbReference type="KEGG" id="ipu:108274327"/>
    <dbReference type="CTD" id="563403"/>
    <dbReference type="OMA" id="SSAPHEW"/>
    <dbReference type="OrthoDB" id="2146116at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050709">
        <property type="term" value="P:negative regulation of protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042058">
        <property type="term" value="P:regulation of epidermal growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000001">
        <property type="entry name" value="Putative inactive rhomboid protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051512">
        <property type="entry name" value="Inactive_Rhomboid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022241">
        <property type="entry name" value="iRhom1_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45965">
        <property type="entry name" value="INACTIVE RHOMBOID PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45965:SF4">
        <property type="entry name" value="INACTIVE RHOMBOID PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12595">
        <property type="entry name" value="iRhom1-2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="410"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="661"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="697"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="722"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="752"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="775"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="809"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="domain" description="Inactive rhomboid protein 1/2 N-terminal" evidence="14">
        <location>
            <begin position="96"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="13">
        <location>
            <begin position="656"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF12595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017339922.1"/>
        </source>
    </evidence>
    <sequence length="860" mass="98276" checksum="B06DA0BD0C9EC51B" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SFT7</accession>
    <name>A0A2D0SFT7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex</fullName>
            <ecNumber evidence="5">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pdhx</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017341115.1">
        <property type="nucleotide sequence ID" value="XM_017485626.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SFT7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035945"/>
    <dbReference type="GeneID" id="108275063"/>
    <dbReference type="KEGG" id="ipu:108275063"/>
    <dbReference type="CTD" id="8050"/>
    <dbReference type="OMA" id="TIKQKPW"/>
    <dbReference type="OrthoDB" id="537444at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000010">
        <property type="entry name" value="Acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000003">
        <property type="entry name" value="Acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="entry name" value="E3-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045257">
        <property type="entry name" value="E2/Pdx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151">
        <property type="entry name" value="DIHYDROLIPOAMIDE ACETYL/SUCCINYL-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151:SF90">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3 5" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="10" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="7">
        <location>
            <begin position="46"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="8">
        <location>
            <begin position="172"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="136"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="214"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="142"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017341115.1"/>
        </source>
    </evidence>
    <sequence length="487" mass="51533" checksum="EC3390C385B7AD2A" modified="2017-12-20" version="1">MAASLRLGHRAVLTVLRFIPFQKCPHVTQSSFRQLFQSAYLLGVAPLKVQMPALSPTMEEGNIVKWLKKEGEAVAAGDALCEIETDKAVVTMESSDDGVLAKILMEEGSKSVRLGTLIALMVEEGEDWKQVEIPPLVSSQPAPAPPLPLAAPTPPASVAPPSRAAPAIGPLRLSPAARHILDTHGLDPHLATATGPRGLITKEDALNLLKEGDAGQPAAPVTAPAPPSPPPLSSPHVPPPAPVSGRPLQPAISVPEKPGAPGTFTEIPASNVRRVIAQRLTQSKTTIPHAYASVDCDLDAVMQLRKELAREEIKVSVNDFIIKAAAVTLKEMPEINVTWSGEGPQALDSIHISIAVATERGLITPIIKDAADKGVQEIATNAKVLAEKARAGKLLPEEYQGGSFSISNLGMFGISGFSAVINPPQACILAVGRSRAELTLAAEGLCTQHLMTVTLSSDGRLVDDELASRFLEKFRNNLENPQRMALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SI37</accession>
    <name>A0A2D0SI37_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Potassium voltage-gated channel subfamily A member 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Voltage-gated potassium channel subunit Kv1.2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">kcna2b</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.2/KCNA2 sub-subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017341946.1">
        <property type="nucleotide sequence ID" value="XM_017486457.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SI37"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020813"/>
    <dbReference type="GeneID" id="108275560"/>
    <dbReference type="KEGG" id="ipu:108275560"/>
    <dbReference type="CTD" id="562853"/>
    <dbReference type="OMA" id="GQPHDHY"/>
    <dbReference type="OrthoDB" id="415460at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043679">
        <property type="term" value="C:axon terminus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:dendrite"/>
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    <dbReference type="GO" id="GO:0044224">
        <property type="term" value="C:juxtaparanode region of axon"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:action potential"/>
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        <property type="term" value="P:protein homooligomerization"/>
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        <property type="entry name" value="Potassium voltage-gated channel subfamily a member"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Potassium voltage-gated channel subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000007">
        <property type="entry name" value="Potassium voltage-gated channel subfamily A member 2"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003972">
        <property type="entry name" value="K_chnl_volt-dep_Kv1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004049">
        <property type="entry name" value="K_chnl_volt-dep_Kv1.2"/>
    </dbReference>
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        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF23">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY A MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01509">
        <property type="entry name" value="KV12CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01496">
        <property type="entry name" value="SHAKERCHANEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="17" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0564">Palmitate</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0630">Potassium</keyword>
    <keyword evidence="9" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="6" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0851">Voltage-gated channel</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00033198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017341946.1"/>
        </source>
    </evidence>
    <sequence length="494" mass="56358" checksum="6CF4AE96ED1F6863" modified="2017-12-20" version="1">MTVATSEHIDEAAAHPGQPQDMYDPEPDHECCERVVINISGLRFETQLKTLSQFPDTLLGDPKKRMRYFDPLRNEYFFDRNRPSFDAILYYYQSGGRLRRPVNVTLDIFSEEIRFYELGEEAIEIFREDEGFIKEEEKLLPENEFQRQVWLLFEYPESSGPARIIAIISVMVILISIVSFCLETLPIFRNEEELRKTYNSDSNSTVISTSNYFTDPFFILETLCIIWFSFEFLVRFFACPSKAGFFVNIMNIIDIVAIIPYFITLGTELAESPEDGQQGQQAMSLAILRVIRLVRVFRIFKLSRHSKGLQILGQTLKASMRELGLLIFFLFIGVILFSSAVYFAEADEPDSQFISIPDAFWWAVVSMTTVGYGDMVPTTIGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYHRETEGEEQAQYLNVTSVPKIDSTEDLKKSRSVSSMSKSDYMEIQEAVNNSNEDFRGENLKTGNCTHANTNYVNITKMLTDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SR80</accession>
    <name>A0A2D0SR80_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Glycerol-3-phosphate acyltransferase 4 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">gpat4</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13">Phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017344805.1">
        <property type="nucleotide sequence ID" value="XM_017489316.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SR80"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000036893"/>
    <dbReference type="GeneID" id="108277038"/>
    <dbReference type="KEGG" id="ipu:108277038"/>
    <dbReference type="CTD" id="137964"/>
    <dbReference type="OrthoDB" id="10051137at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07991">
        <property type="entry name" value="LPLAT_LPCAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045252">
        <property type="entry name" value="LPCAT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063:SF37">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063">
        <property type="entry name" value="PHOSPHOLIPID ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="15">
        <location>
            <begin position="240"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017344805.1"/>
        </source>
    </evidence>
    <sequence length="454" mass="51398" checksum="CBBD60FA1184B33A" modified="2017-12-20" version="1">MESYLFPFDGLICMLLGISFTVWFTLLLVFIIVPAVFGMSFGIRRLYMNSLLKIFEWATLRMERGAKEKNQRLYKPYSNGIIAKEPASLEQEIQEVRRLGGAEAEFDMSDILFFCRRGVESIVDDEVTKRFTAEELESWNLLTRSNYNYNFQNISRRLTALWGLGVLIRYGILLPLRVTLAFTGVGLLVVLTSLIGLLPNGGMKNFLSEKAHLMCYRICVRALTAIITYHDSENKPKNGGICVANHTSPIDVIILASDGCYAMVGQVHGGLMGVIQRAMVKACPHIWFERSEVKDRHLVAKRLSDHVADKTKLPILIFPEGTCINNTSVMMFKKGSFEIGCTVYPVAIKYDPRFGDAFWNSSKFGMVNYLLHMMSSWAIVCSVWYLPPMSREEDEDAVQFANRVKAAIARKGGLADLLWDGGLKREKVKEVFKEEQQKLYSKVLVGNGEDRSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SRA8</accession>
    <name>A0A2D0SRA8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G protein-coupled receptor kinase</fullName>
            <ecNumber evidence="11">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">grk5</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[G-protein-coupled receptor] + ATP = [G-protein-coupled receptor]-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12008"/>
            <dbReference type="Rhea" id="RHEA-COMP:11260"/>
            <dbReference type="Rhea" id="RHEA-COMP:11261"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_017345228.1">
        <property type="nucleotide sequence ID" value="XM_017489739.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SRA8"/>
    <dbReference type="GeneID" id="108277214"/>
    <dbReference type="CTD" id="2869"/>
    <dbReference type="OrthoDB" id="354826at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050254">
        <property type="term" value="F:rhodopsin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000009">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000074">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000239">
        <property type="entry name" value="GPCR_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355:SF26">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00717">
        <property type="entry name" value="GPCRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RGS" evidence="14">
        <location>
            <begin position="58"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="180"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="15">
        <location>
            <begin position="443"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="526"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="570"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="543"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="570"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600239-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017345228.1"/>
        </source>
    </evidence>
    <sequence length="599" mass="69011" checksum="5097456487B54E8B" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SUI4</accession>
    <name>A0A2D0SUI4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">RWD domain-containing protein 2B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">rwdd2b</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3 4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017346354.1">
        <property type="nucleotide sequence ID" value="XM_017490865.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017346355.1">
        <property type="nucleotide sequence ID" value="XM_017490866.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SUI4"/>
    <dbReference type="GeneID" id="108277868"/>
    <dbReference type="KEGG" id="ipu:108277868"/>
    <dbReference type="CTD" id="10069"/>
    <dbReference type="GeneTree" id="ENSGT00390000007224"/>
    <dbReference type="OrthoDB" id="432412at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="CDD" id="cd23829">
        <property type="entry name" value="RWD_RWDD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd24163">
        <property type="entry name" value="RWDD2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017359">
        <property type="entry name" value="Phi-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010541">
        <property type="entry name" value="Prp3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059181">
        <property type="entry name" value="RWDD2A-B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15955">
        <property type="entry name" value="RWD DOMAIN CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15955:SF8">
        <property type="entry name" value="RWD DOMAIN-CONTAINING PROTEIN 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06544">
        <property type="entry name" value="Prp3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038021">
        <property type="entry name" value="UCP038021_RWDD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RWD" evidence="1">
        <location>
            <begin position="12"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017346354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017346355.1"/>
        </source>
    </evidence>
    <sequence length="288" mass="32591" checksum="7083768ADDD84FEB" modified="2017-12-20" version="1">MDQTEHAEAQLAEVELLLSMFPSQEELEVEQIAYAELRAYVEGAADCPPNTRPELCVKIRTHTGVDVSLSCTYPSDYPAVPPEIVVSAMCRRCGALSRAQHARIVSDLRSYLRESCTGDVCVLSAVDWVRDHTHKYLEEEDDSTKGAAETQCTETFTRLWIYSHHIYNKSKRKNILEWAKELQLSGFSMPGKPGVVCVEGLQAACEEFWARVKVLTWKRIMIRHREDIPLGPAGQSAESLRRFDGFEETAFDPHGNRGNHMDLGQLFQFLSERGCGQIFQLYFGVEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SVB7</accession>
    <name>A0A2D0SVB7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA repair protein XRCC1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">X-ray repair cross-complementing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18 19" type="primary">xrcc1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18 19" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18 19">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Scaffold protein involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes. Negatively regulates ADP-ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity. Recognizes and binds poly-ADP-ribose chains: specifically binds auto-poly-ADP-ribosylated PARP1, limiting its activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer. Interacts with polynucleotide kinase (PNK), DNA polymerase-beta (POLB) and DNA ligase III (LIG3). Interacts with APTX and APLF. Interacts with APEX1; the interaction is induced by SIRT1 and increases with the acetylated form of APEX1. Interacts with (poly-ADP-ribosylated) PARP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017346629.1">
        <property type="nucleotide sequence ID" value="XM_017491140.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017346630.1">
        <property type="nucleotide sequence ID" value="XM_017491141.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SVB7"/>
    <dbReference type="GeneID" id="108278020"/>
    <dbReference type="KEGG" id="ipu:108278020"/>
    <dbReference type="CTD" id="7515"/>
    <dbReference type="OrthoDB" id="25840at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000012">
        <property type="term" value="P:single strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17725">
        <property type="entry name" value="BRCT_XRCC1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17707">
        <property type="entry name" value="BRCT_XRCC1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000025">
        <property type="entry name" value="DNA repair protein XRCC1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000008">
        <property type="entry name" value="X-ray repair cross complementing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000012">
        <property type="entry name" value="X-ray repair cross complementing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045080">
        <property type="entry name" value="BRCT_XRCC1_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002706">
        <property type="entry name" value="Xrcc1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11370:SF5">
        <property type="entry name" value="DNA REPAIR PROTEIN XRCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11370">
        <property type="entry name" value="DNA-REPAIR PROTEIN XRCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01834">
        <property type="entry name" value="XRCC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="305"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="517"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="218"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="396"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="239"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="274"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="288"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="399"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="411"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="434"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="446"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="467"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="503"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00064453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00079580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017346629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017346630.1"/>
        </source>
    </evidence>
    <sequence length="610" mass="68728" checksum="F8F38AD37CCDA91C" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SY66</accession>
    <name>A0A2D0SY66_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Vacuolar protein sorting-associated protein 29</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 13">Vesicle protein sorting 29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">vps29</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the commander complex that is essential for endosomal recycling of transmembrane cargos; the commander complex is composed of the Csubcomplex and the retriever subcomplex. Component of the retriever complex, which is a heterotrimeric complex related to retromer cargo-selective complex (CSC) and essential for retromer-independent retrieval and recycling of numerous cargos. Component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. In the endosomes, retriever complex drives the retrieval and recycling of NxxY-motif-containing cargo proteins by coupling to snx17, a cargo essential for the homeostatic maintenance of numerous cell surface proteins associated with processes that include cell migration, cell adhesion, nutrient supply and cell signaling. The recruitment of the retriever complex to the endosomal membrane involves Cand WASH complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the VPS29 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347270.1">
        <property type="nucleotide sequence ID" value="XM_017491781.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SY66"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019659"/>
    <dbReference type="GeneID" id="100528207"/>
    <dbReference type="CTD" id="51699"/>
    <dbReference type="OrthoDB" id="10258130at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030904">
        <property type="term" value="C:retromer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07394">
        <property type="entry name" value="MPP_Vps29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000009">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028661">
        <property type="entry name" value="Vps29"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11124">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN VPS29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="14">
        <location>
            <begin position="58"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017347270.1"/>
        </source>
    </evidence>
    <sequence length="237" mass="26835" checksum="7CA53BE035CAC2F3" modified="2017-12-20" version="1">MMDDVALQLLYLLSSLFASLKINVTLYDLQIAFCFPKWMECCCSDCTFFTDAQYRDLVLVLGDLHIPHRCNTLPAKFKKLLVPGKIQHILCTGNLCTKESYDYLKTLAGDVHIVRGDFDENLNYPEQKVVTVGQFKIGLIHGHQVIPWGDMASLALLQRQLDVDILISGHTHKFEAFENENKFYINPGSATGAYNALESNIIPSFVLMDIQASTVVTYVYQLIGDDVKVERIEYKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SYY5</accession>
    <name>A0A2D0SYY5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="22">A disintegrin and metalloproteinase with thrombospondin motifs 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">adamts2a</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347550.1">
        <property type="nucleotide sequence ID" value="XM_017492061.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SYY5"/>
    <dbReference type="GeneID" id="108278612"/>
    <dbReference type="KEGG" id="ipu:108278612"/>
    <dbReference type="CTD" id="571682"/>
    <dbReference type="OMA" id="DGEMNNC"/>
    <dbReference type="OrthoDB" id="5855429at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04273">
        <property type="entry name" value="ZnMc_ADAMTS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000006">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.830:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1620.60:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000008">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1620.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013273">
        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041645">
        <property type="entry name" value="ADAMTS_CR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045371">
        <property type="entry name" value="ADAMTS_CR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002870">
        <property type="entry name" value="Peptidase_M12B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010909">
        <property type="entry name" value="PLAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF141">
        <property type="entry name" value="A DISINTEGRIN AND METALLOPROTEINASE WITH THROMBOSPONDIN MOTIFS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17771">
        <property type="entry name" value="ADAMTS_CR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19236">
        <property type="entry name" value="ADAMTS_CR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01562">
        <property type="entry name" value="Pep_M12B_propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50900">
        <property type="entry name" value="PLAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15" id="KW-0106">Calcium</keyword>
    <keyword evidence="12 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 15 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 22" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="9 15 17" id="KW-0862">Zinc</keyword>
    <keyword evidence="11" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase M12B" evidence="19">
        <location>
            <begin position="249"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="PLAC" evidence="20">
        <location>
            <begin position="1036"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1086"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="active site" evidence="14 17">
        <location>
            <position position="392"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15 17">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15 17">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15 17">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="451"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="451"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="326"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="369"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="408"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="475"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="486"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="495"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="522"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="556"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="560"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="571"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017347550.1"/>
        </source>
    </evidence>
    <sequence length="1277" mass="144634" checksum="D64838273A52037C" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SZ75</accession>
    <name>A0A2D0SZ75_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cysteine protease</fullName>
            <ecNumber evidence="13">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">atg4a</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>[protein]-C-terminal L-amino acid-glycyl-phosphatidylethanolamide + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine</text>
            <dbReference type="Rhea" id="RHEA:67548"/>
            <dbReference type="Rhea" id="RHEA-COMP:17323"/>
            <dbReference type="Rhea" id="RHEA-COMP:17324"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:172940"/>
            <dbReference type="ChEBI" id="CHEBI:172941"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:67549"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the peptidase C54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="13"/>
    <dbReference type="RefSeq" id="XP_017347640.1">
        <property type="nucleotide sequence ID" value="XM_017492151.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SZ75"/>
    <dbReference type="GeneID" id="108278677"/>
    <dbReference type="KEGG" id="ipu:108278677"/>
    <dbReference type="CTD" id="115201"/>
    <dbReference type="OrthoDB" id="2960936at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019786">
        <property type="term" value="F:protein-phosphatidylethanolamide deconjugating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005078">
        <property type="entry name" value="Peptidase_C54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046792">
        <property type="entry name" value="Peptidase_C54_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624:SF35">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03416">
        <property type="entry name" value="Peptidase_C54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 13 17" id="KW-0645">Protease</keyword>
    <keyword evidence="9 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Peptidase C54 catalytic" evidence="15">
        <location>
            <begin position="48"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="200"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017347640.1"/>
        </source>
    </evidence>
    <sequence length="426" mass="48485" checksum="E144BFA42EBAD229" modified="2017-12-20" version="1">MEAGLFVPVLAKYENQINVFSEFLDDLPDTEDPVWVLGQSYHVKTEKSELLSDVCSRLWFTYRRKFSPIGGTGPSSDTGWGCMLRCGQMILAQALVCSHLGRTWRWVSEESQPAEYQRILHCFLDRKDSCYSIHQMAQMGVGEGKSVGEWYGPNTVAQVLRKLTLFDDWSSLAVYVSMDNTVVIEDIKKQCHQEASSSRCIRGGVEVPPSGESSSGHKLTDDREQRLAPSPVSHSQQYSQQHSQHSLDWRPLLLLIPLRMGINNINPIYIQALKECFKMPQSCGVLGGKPNLAYYFIGFIDDELIYLDPHTTQSAVDSETDLGVDDQSYHCQRSPHRMKITSLDPSVALGFFCKSEEDFDSWCNQVQQEILKKRNLRMFELVEKHPSHWPPFIPPTKPEVQTTGAEFIESTDKLFESEEEFEILNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T0T3</accession>
    <name>A0A2D0T0T3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Centrosomal protein 57kDa-like protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Cep57-related protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">cep57l1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the translokin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017348529.1">
        <property type="nucleotide sequence ID" value="XM_017493040.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T0T3"/>
    <dbReference type="GeneID" id="108279073"/>
    <dbReference type="KEGG" id="ipu:108279073"/>
    <dbReference type="CTD" id="285753"/>
    <dbReference type="OrthoDB" id="76453at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051756">
        <property type="entry name" value="Centrosomal_MT-associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025913">
        <property type="entry name" value="Cep57_CLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024957">
        <property type="entry name" value="Cep57_MT-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19336:SF10">
        <property type="entry name" value="CENTROSOMAL PROTEIN CEP57L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19336">
        <property type="entry name" value="UNCHARACTERIZED DUF1167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14073">
        <property type="entry name" value="Cep57_CLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06657">
        <property type="entry name" value="Cep57_MT_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cep57 centrosome localisation" evidence="12">
        <location>
            <begin position="64"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Cep57 centrosome microtubule-binding" evidence="11">
        <location>
            <begin position="337"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="151"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="263"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="305"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="394"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="62"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="365"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="306"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="408"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017348529.1"/>
        </source>
    </evidence>
    <sequence length="478" mass="54571" checksum="BA0545A30E37A828" modified="2017-12-20" version="1">MDHLRDSSLCLDSPSKHSYIGSFYAPPDRIPSQLMVQHQVKPSSTLVKPSRLTQHTVPDSGSRAVIAALKTLQEKMRRLELERVQAEKNVERFAQAAHQFASSGNQTSQQLQREGESSKRKELVSQLHSAEARCSLLEKQLEYMRKMVESAQRETTAPVDREECVQQKKKNKRTEPQIQAQLQKLEEECIKLTNTQSEAERKIKLLERKLLEEEHERKLVQEKAEELQRELEANLLFSAAEVKPKKLKKQTKEKTLTKKSVNTEVPRHAPKPKRMPFVAGTSTSPSHSVNANVQTVLHLLKTRQPRMCERTQSEPKKCSGSHRAPQSPSDGAAAMGSLSELLLALQDELGHMSFEHQELVRQIDETDQRELREDLERELDCLVRRMEEKAAQITKLRKHQQTLSQRSPKQKPKPKPRRAASADSRCVSRVQVVPSCVGKATPSRPQRHQSAGHGRLQLLRDAQKMQSSLRQRDISWET</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T809</accession>
    <name>A0A2D0T809_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Junctophilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">jph1b</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Junctophilins contribute to the formation of junctional membrane complexes (JMCs) which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. Provides a structural foundation for functional cross-talk between the cell surface and intracellular calcium release channels.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the junctophilin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017351019.1">
        <property type="nucleotide sequence ID" value="XM_017495530.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017351020.1">
        <property type="nucleotide sequence ID" value="XM_017495531.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T809"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000002006"/>
    <dbReference type="GeneID" id="108280498"/>
    <dbReference type="KEGG" id="ipu:108280498"/>
    <dbReference type="CTD" id="556485"/>
    <dbReference type="GeneTree" id="ENSGT00940000156130"/>
    <dbReference type="OMA" id="FSAQHNA"/>
    <dbReference type="OrthoDB" id="284854at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030314">
        <property type="term" value="C:junctional membrane complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016529">
        <property type="term" value="C:sarcoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000001">
        <property type="entry name" value="Junctophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000003">
        <property type="entry name" value="Junctophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017191">
        <property type="entry name" value="Junctophilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23085">
        <property type="entry name" value="GH28348P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23085:SF6">
        <property type="entry name" value="JUNCTOPHILIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037387">
        <property type="entry name" value="Junctophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="650"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="166"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="226"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="438"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="560"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="246"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="259"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="459"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="565"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="605"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017351019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017351020.1"/>
        </source>
    </evidence>
    <sequence length="671" mass="72787" checksum="A8670B28B4B55E1F" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T876</accession>
    <name>A0A2D0T876_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14 15">Piwi-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">LOC108280521</name>
    </gene>
    <organism evidence="13 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the argonaute family. Piwi subfamily.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017495571.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017351061.1">
        <property type="nucleotide sequence ID" value="XM_017495572.3"/>
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    <dbReference type="STRING" id="7998.ENSIPUP00000024370"/>
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    <dbReference type="KEGG" id="ipu:108280521"/>
    <dbReference type="GeneTree" id="ENSGT00950000183200"/>
    <dbReference type="OrthoDB" id="445936at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043186">
        <property type="term" value="C:P granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="CDD" id="cd02845">
        <property type="entry name" value="PAZ_piwi_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04658">
        <property type="entry name" value="Piwi_piwi-like_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Piwi-like RNA-mediated gene silencing 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Piwi-like RNA-mediated gene silencing 2"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014811">
        <property type="entry name" value="ArgoL1"/>
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    <dbReference type="InterPro" id="IPR031320">
        <property type="entry name" value="GAGE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
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        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08699">
        <property type="entry name" value="ArgoL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05831">
        <property type="entry name" value="GAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23278">
        <property type="entry name" value="Piwi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00949">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8" id="KW-0469">Meiosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="PAZ" evidence="11">
        <location>
            <begin position="277"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="12">
        <location>
            <begin position="553"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017351060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017351061.1"/>
        </source>
    </evidence>
    <sequence length="859" mass="97747" checksum="C59CA663DF96FBBB" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T9P3</accession>
    <name>A0A2D0T9P3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Synaptotagmin-C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">syt3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptotagmin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017351589.1">
        <property type="nucleotide sequence ID" value="XM_017496100.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T9P3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013408"/>
    <dbReference type="GeneID" id="108280740"/>
    <dbReference type="KEGG" id="ipu:108280740"/>
    <dbReference type="CTD" id="84258"/>
    <dbReference type="OMA" id="SHNERCD"/>
    <dbReference type="OrthoDB" id="67700at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070382">
        <property type="term" value="C:exocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017156">
        <property type="term" value="P:calcium-ion regulated exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08385">
        <property type="entry name" value="C2A_Synaptotagmin-1-5-6-9-10"/>
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    </dbReference>
    <dbReference type="CDD" id="cd08403">
        <property type="entry name" value="C2B_Synaptotagmin-3-5-6-9-10"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Synaptotagmin 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000011">
        <property type="entry name" value="Synaptotagmin 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001565">
        <property type="entry name" value="Synaptotagmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF176">
        <property type="entry name" value="SYNAPTOTAGMIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00399">
        <property type="entry name" value="SYNAPTOTAGMN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="178"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="178"/>
            <end position="192"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="248"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017351589.1"/>
        </source>
    </evidence>
    <sequence length="559" mass="61370" checksum="F2A8AC5E894D984B" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0TB47</accession>
    <name>A0A2D0TB47_ICTPU</name>
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        <submittedName>
            <fullName evidence="4">Pro-FMRFamide-related neuropeptide FF like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">npffl</name>
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    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017352088.1">
        <property type="nucleotide sequence ID" value="XM_017496599.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0TB47"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000036823"/>
    <dbReference type="GeneID" id="108281053"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="InterPro" id="IPR008065">
        <property type="entry name" value="NPFF"/>
    </dbReference>
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        <property type="entry name" value="NEUROPEPTIDE FF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRO-FMRFAMIDE-RELATED NEUROPEPTIDE FF"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FMRFAMIDEPEP"/>
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    <keyword evidence="4" id="KW-0527">Neuropeptide</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="20"/>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="83"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017352088.1"/>
        </source>
    </evidence>
    <sequence length="117" mass="13223" checksum="6B6779E483CE0918" modified="2017-12-20" version="1">MDASVWFVVVLVIAGATHGVTDGDTAVDSEENMTETLVSEQQLRGVIDDRLLKMALSSLLHNFQRNTRDPSVLHHPQRFGRGSHSDVRTDERIQSRDWDSVPQQIWSLAVPQRFGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979E292</accession>
    <name>A0A979E292_ICTPU</name>
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        <submittedName>
            <fullName evidence="15">HERV-H LTR-associating protein 2 isoform X1</fullName>
        </submittedName>
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        <name evidence="15" type="primary">hhla2b.2</name>
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        <name type="scientific">Ictalurus punctatus</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_047006297.1">
        <property type="nucleotide sequence ID" value="XM_047150341.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979E292"/>
    <dbReference type="GeneID" id="124626238"/>
    <dbReference type="KEGG" id="ipu:124626238"/>
    <dbReference type="CTD" id="103909756"/>
    <dbReference type="OrthoDB" id="9983389at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042130">
        <property type="term" value="P:negative regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042102">
        <property type="term" value="P:positive regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031295">
        <property type="term" value="P:T cell costimulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000142">
        <property type="entry name" value="V-set domain-containing T-cell activation inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051713">
        <property type="entry name" value="T-cell_Activation_Regulation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466:SF14">
        <property type="entry name" value="BUTYROPHILIN SUBFAMILY 2 MEMBER A2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466">
        <property type="entry name" value="T-LYMPHOCYTE ACTIVATION ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5038022707" evidence="12">
        <location>
            <begin position="20"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="3"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="138"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="226"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_047006297.1"/>
        </source>
    </evidence>
    <sequence length="363" mass="40781" checksum="FAAE2D898F67C163" modified="2023-02-22" version="1">MKPVILTFVLFWSIPLTTEEDVTVYCIVTSNCVLPCTSTYHDIIHWYKEGKADSVHTFYNKADDLQYQDVDFKGRTSLFKDQIPQGNASLLLSSFRTEDQGKYKCYTATNSENREQFVLLKIKVPVKWVDLKMTSEGITCSTKDVYPEPNISWFRDGRSEDKFTVNRTGDKEGLFSVSSVLTQSVAENTTYSCSISLRDESQHYTASLRKENVEIYPGHNVTIHCPVSQDNTGNSNITLTYGESSTTLTYTQTSQLPTTTVQWRGKTLHLARDGTVTIHNPENVEYTGTYTCERLTAQSRHVVHTEVKICSANTIRIALGVTVAIVVVTIAAVCLIMWKRANNMRNRGESDVNPHNGEGNGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EER7</accession>
    <name>A0A979EER7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Calmodulin-binding transcription activator 1</fullName>
        </recommendedName>
    </protein>
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        <name evidence="18" type="primary">LOC108254811</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Transcriptional activator.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">May interact with calmodulin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CAMTA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047005375.1">
        <property type="nucleotide sequence ID" value="XM_047149419.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108254811"/>
    <dbReference type="KEGG" id="ipu:108254811"/>
    <dbReference type="OrthoDB" id="407555at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.190:FF:000038">
        <property type="entry name" value="calmodulin-binding transcription activator 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000165">
        <property type="entry name" value="calmodulin-binding transcription activator 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000089">
        <property type="entry name" value="calmodulin-binding transcription activator 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005559">
        <property type="entry name" value="CG-1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23335:SF11">
        <property type="entry name" value="CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23335">
        <property type="entry name" value="CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR CAMTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03859">
        <property type="entry name" value="CG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01076">
        <property type="entry name" value="CG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51437">
        <property type="entry name" value="CG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7 14" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CG-1" evidence="16">
        <location>
            <begin position="22"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="1189"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="244"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="546"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="612"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="717"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="776"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1100"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1294"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1330"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="256"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="281"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="311"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="612"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="632"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="732"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="781"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="802"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="1111"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
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            <end position="1154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1300"/>
            <end position="1317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1335"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1353"/>
            <end position="1362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_047005375.1"/>
        </source>
    </evidence>
    <sequence length="1766" mass="189887" checksum="14DCF269272C1E7B" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979ELP5</accession>
    <name>A0A979ELP5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="2">B-type lectin plumieribetin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC108264533</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SDA103</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047009511.1">
        <property type="nucleotide sequence ID" value="XM_047153555.2"/>
    </dbReference>
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    <dbReference type="GeneID" id="108264533"/>
    <dbReference type="KEGG" id="ipu:108264533"/>
    <dbReference type="OrthoDB" id="1884773at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_047009511.1"/>
        </source>
    </evidence>
    <sequence length="112" mass="12727" checksum="5D73DCF6D6774BAE" modified="2023-02-22" version="1">MSRNFLSMNSELKKGDFLLSNNGEYKAVFQEDGNFVVYGWKPVWQSNTSGSTAKRLIMQEDCNLVMYDDGPGLWHTNTHHSGVKVCRLTLRNDGMLVVENDGKVVWKSTPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EN69</accession>
    <name>A0A979EN69_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Oxytocin-neurophysin 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">oxt</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the vasopressin/oxytocin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047007911.1">
        <property type="nucleotide sequence ID" value="XM_047151955.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EN69"/>
    <dbReference type="GeneID" id="108260424"/>
    <dbReference type="CTD" id="5020"/>
    <dbReference type="OrthoDB" id="10056056at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005185">
        <property type="term" value="F:neurohypophyseal hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.9.10:FF:000001">
        <property type="entry name" value="oxytocin-neurophysin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.9.10">
        <property type="entry name" value="Neurohypophysial hormone domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000981">
        <property type="entry name" value="Neurhyp_horm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036387">
        <property type="entry name" value="Neurhyp_horm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022423">
        <property type="entry name" value="Neurohypophysial_hormone_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11681:SF5">
        <property type="entry name" value="ISOTOCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11681">
        <property type="entry name" value="NEUROPHYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00184">
        <property type="entry name" value="Hormone_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001815">
        <property type="entry name" value="Nonapeptide_hormone_precursor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00831">
        <property type="entry name" value="NEUROPHYSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00003">
        <property type="entry name" value="NH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49606">
        <property type="entry name" value="Neurophysin II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00264">
        <property type="entry name" value="NEUROHYPOPHYS_HORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0027">Amidation</keyword>
    <keyword evidence="2" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="6 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0372">Hormone</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="38"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="59"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="62"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="77"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="110"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="124"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001815-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_047007911.1"/>
        </source>
    </evidence>
    <sequence length="168" mass="17541" checksum="C6DCBBC090EE2E17" modified="2023-02-22" version="1">MGQSCFGGTRGAYTILKMSAAPLSVFCLLCLLSICTACYISNCPIGGKRAVQDIPTRQCMACGPGDKGRCFGPNICCGEEIGCMVGTLEAMRCLEEDYVPSPCETGGKTCGSVAGRCAAPGVCCDSESCTTDPSCLEGEGDAAAHSLPASGKDLLLKLLHWTNYRPYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EU07</accession>
    <name>A0A979EU07_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Anaphase-promoting complex subunit 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Cyclosome subunit 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21 22" type="primary">anapc5</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21 22" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21 22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the APC5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047011297.2">
        <property type="nucleotide sequence ID" value="XM_047155341.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053535992.1">
        <property type="nucleotide sequence ID" value="XM_053680017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EU07"/>
    <dbReference type="GeneID" id="108264899"/>
    <dbReference type="KEGG" id="ipu:108264899"/>
    <dbReference type="CTD" id="51433"/>
    <dbReference type="OrthoDB" id="2504561at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16270">
        <property type="entry name" value="Apc5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037679">
        <property type="entry name" value="Apc5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026000">
        <property type="entry name" value="Apc5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048968">
        <property type="entry name" value="Apc5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12830">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12830:SF9">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12862">
        <property type="entry name" value="ANAPC5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21371">
        <property type="entry name" value="Apc5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 5 N-terminal" evidence="19">
        <location>
            <begin position="30"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 5" evidence="18">
        <location>
            <begin position="261"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 5" evidence="18">
        <location>
            <begin position="466"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF12862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF21371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_047011297.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_053535992.1"/>
        </source>
    </evidence>
    <sequence length="762" mass="86243" checksum="01FFE0F134E266BB" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EYQ4</accession>
    <name>A0A979EYQ4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transmembrane inner ear expressed protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tmie</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047012923.1">
        <property type="nucleotide sequence ID" value="XM_047156967.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EYQ4"/>
    <dbReference type="GeneID" id="108267261"/>
    <dbReference type="KEGG" id="ipu:108267261"/>
    <dbReference type="CTD" id="259236"/>
    <dbReference type="OrthoDB" id="6154284at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042472">
        <property type="term" value="P:inner ear morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032006">
        <property type="entry name" value="TMIE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28635">
        <property type="entry name" value="TRANSMEMBRANE INNER EAR EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28635:SF1">
        <property type="entry name" value="TRANSMEMBRANE INNER EAR EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16038">
        <property type="entry name" value="TMIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_047012923.1"/>
        </source>
    </evidence>
    <sequence length="165" mass="18012" checksum="6A34EB7DADF870AC" modified="2023-02-22" version="1">MAMDWPTHPPPALVPAVVSAGAVLVSHYVFNAAAQIPDPALLPTDPPPKPDPITSETVVFWGLRLWQVVGIFSMFVLAIIIILCCIFKCRVPRTKKEIEARHAQRLAAKSYANTLETVPPLNELTEIPGVICTVSEHVQTRDGNQLPALNEEDEPTNQSAQNETG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F0Y3</accession>
    <name>A0A979F0Y3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Gamma-aminobutyric acid receptor subunit rho-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">GABA(A) receptor subunit rho-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC108270165</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">SDA103</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="18">Three rho subunits (rho-1/GBRR1, rho-2/GBRR2 and rho-3/GBRR3) coassemble either to form functional homopentamers or heteropentamers. Rho-1/GBRR1 subunits can also associate with alpha-1/GBRA1 subunits to form a functional GABAAR. Interacts with SQSTM1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRR1 sub-subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047013733.2">
        <property type="nucleotide sequence ID" value="XM_047157777.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979F0Y3"/>
    <dbReference type="GeneID" id="108270165"/>
    <dbReference type="KEGG" id="ipu:108270165"/>
    <dbReference type="OrthoDB" id="442503at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19059">
        <property type="entry name" value="LGIC_TM_GABAAR_rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000015">
        <property type="entry name" value="gamma-aminobutyric acid receptor subunit rho-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000005">
        <property type="entry name" value="Putative gamma-aminobutyric acid receptor subunit rho-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0868">Chloride</keyword>
    <keyword evidence="10" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 21" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 21" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="25" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 21" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="194"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="375"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="22">
        <location>
            <begin position="2"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="23">
        <location>
            <begin position="201"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00061453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00064308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00070181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00076172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_047013733.2"/>
        </source>
    </evidence>
    <sequence length="393" mass="45788" checksum="09332F9885BD36D4" modified="2023-06-28" version="1">MRPAFGGPPIPVGVDVQVESLDTISEVDMDFTMTLYLRHYWKDERLSFPSTNNQSMTFDSRLVKKIWVPDMFFVHSKRSFIHDTTTDNVMLRVYPDGNVLYSLRVTVTAMCNMDLSRFPLDTQTCSLEIESYAYTDDDLMLYWKKGNESLNTDDRISLSQFLIQKFHTTTKLAFYSSTGWYNRLYIHFTLRRHIFFFLLQTYFPATLMVMLSWVSFWIDRRAVPARVPLGITTVLTMSTIITGVNASMPRVSYIKAVDIYLWVSFVFVFLSVIEYAAVNYLTTVQERTERKLREQLPCTCGIAHPNEHMMVTTAYSDVDQSTTGNYGMPDNEVRQERIFAQFTLADNQTTSHVRSLRSYVNIWIDTHAIDKYSRVIFPGAYTLFNIIYWSIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F213</accession>
    <name>A0A979F213_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Plasmalemma vesicle associated protein b isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">plvapb</name>
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    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047014125.1">
        <property type="nucleotide sequence ID" value="XM_047158169.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979F213"/>
    <dbReference type="GeneID" id="108271200"/>
    <dbReference type="CTD" id="570276"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002693">
        <property type="term" value="P:positive regulation of cellular extravasation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043114">
        <property type="term" value="P:regulation of vascular permeability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009538">
        <property type="entry name" value="PV-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21687">
        <property type="entry name" value="PLASMALEMMA VESICLE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21687:SF6">
        <property type="entry name" value="PLASMALEMMA VESICLE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5038122906" evidence="3">
        <location>
            <begin position="30"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="352"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="155"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="262"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="370"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_047014125.1"/>
        </source>
    </evidence>
    <sequence length="384" mass="43441" checksum="61C0EC8A8FE6BEC7" modified="2023-02-22" version="1">MYNNNYTRPKSLIIVSLVLFLVYGQPAKSADEKRVEELELSFSKLSSDNTNLRKEKADLIAMLKTKTAEKDAADKKVVKITADLDAAKVNNTKCLQALAICNKPPIVRCPGTLIPSSVTNEHVRTLQILLDQQKSLYAILQANFSQTVQNLRFDLDNAVKDKNKHEMAMIKLKQEKDDLTSELQLYKKKCKEDFVMSLRGIQDVTTAFLTKIDNLFPDTFTFHLTCSKQQEQMQKIHANCTNLSRQVEDKFQNYLNSVGDKVSALQARSSRLEVENKRLTSDNQNCSQSRAQDAEKCLKRLQEAQESHDNLMESQLNRYKQLLQEKQRLDTTCITKPPVPKPNGMDPYMIYGQSAGNRLGQMGVPGLHTAVQSKPGSSQPPKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979FDR3</accession>
    <name>A0A979FDR3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribonuclease inhibitor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC108279561</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047018171.2">
        <property type="nucleotide sequence ID" value="XM_047162215.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979FDR3"/>
    <dbReference type="GeneID" id="108279561"/>
    <dbReference type="KEGG" id="ipu:108279561"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_047018171.2"/>
        </source>
    </evidence>
    <sequence length="224" mass="24133" checksum="0221FA65760B70AF" modified="2023-06-28" version="2">MLCSKLKGERSKSPAPSCISMKSDQSMEPPLKFKAEGPSDLHSISYDIISMRSWSALVSSLSSETSNLRELHLTVKTLDLSRNELGHSGLKSLCAVLENPHCKVETLRLCGCGVSDEGCAALTSALKSNPSHLRQLYLSDNKIGDSGVKSLSAVLENPLCKLEILGLRGCGVSDEGCAALTSALRSNPSHLRKLNLFDNKIGDSGKKLLSALKDEHCKRLELIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QWM2</accession>
    <name>A0A9F7QWM2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">zgc:112183</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_053530286.1">
        <property type="nucleotide sequence ID" value="XM_053674311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QWM2"/>
    <dbReference type="GeneID" id="108278300"/>
    <dbReference type="KEGG" id="ipu:108278300"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04112">
        <property type="entry name" value="Rab26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000459">
        <property type="entry name" value="ras-related protein Rab-37 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_053530286.1"/>
        </source>
    </evidence>
    <sequence length="230" mass="26029" checksum="F08EE158906C7496" modified="2023-06-28" version="1">MSTKKAPMSDNEVKNGTSRSMLERCASVNEYFDISFKVMLLGDSAVGKTCVLVRFKDGAFLGGNFIATVGIDFRNKVVTVDNMKVKLQIWDTAGQERFRSVTHAYYRDAQALLLLYDITRKSSFDNIRAWLTEIHEYAQKDVVIMLLGNKSDMAAERVIKPEEGQKLAKEYGVPFMETSAKTGVNVELAFLAIARQLKHRSAQQPHEPKFQIHDYIETQKQKSNCCSSLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QXC3</accession>
    <name>A0A9F7QXC3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Apoptosis regulator BAX isoform X2</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="6 7">Apoptosis regulator BAX-like isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC128629196</name>
        <name evidence="5" type="synonym">LOC128629195</name>
        <name evidence="7" type="synonym">LOC128629197</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5 6 7" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5 6 7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Bcl-2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053532081.1">
        <property type="nucleotide sequence ID" value="XM_053676106.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053532089.1">
        <property type="nucleotide sequence ID" value="XM_053676114.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053532094.1">
        <property type="nucleotide sequence ID" value="XM_053676119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QXC3"/>
    <dbReference type="GeneID" id="128629196"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051400">
        <property type="term" value="F:BH domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097192">
        <property type="term" value="P:extrinsic apoptotic signaling pathway in absence of ligand"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008630">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008053">
        <property type="term" value="P:mitochondrial fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001836">
        <property type="term" value="P:release of cytochrome c from mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06845">
        <property type="entry name" value="Bcl-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.437.10">
        <property type="entry name" value="Blc2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036834">
        <property type="entry name" value="Bcl-2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046371">
        <property type="entry name" value="Bcl-2_BH1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026298">
        <property type="entry name" value="Bcl-2_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002475">
        <property type="entry name" value="Bcl2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256:SF42">
        <property type="entry name" value="APOPTOSIS REGULATOR BAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256">
        <property type="entry name" value="BCL-2 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00452">
        <property type="entry name" value="Bcl-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01862">
        <property type="entry name" value="BCL2FAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00337">
        <property type="entry name" value="BCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56854">
        <property type="entry name" value="Bcl-2 inhibitors of programmed cell death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50062">
        <property type="entry name" value="BCL2_FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bcl-2 Bcl-2 homology region 1-3" evidence="3">
        <location>
            <begin position="63"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053532081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_053532089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053532094.1"/>
        </source>
    </evidence>
    <sequence length="190" mass="21372" checksum="D78FE74E69976F93" modified="2023-06-28" version="1">MAAALSGEGDGPDQILEVGAVLLKDFIYERVHRHGDSGTVVSRHELGGSELCDPTHKKLAQCLQQIGDELDNNVDLQRMLADSALQPTKEVFVKVAREIFSDGKFNWGRVVALFYFACRLVIKALLTKIPDIIRTIINWTLDYLREHVINWIREQGGWEGIQTYFGTPTWKTVGVFLAGVLTTVLVMRKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QXF9</accession>
    <name>A0A9F7QXF9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">NLR family CARD domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC128630377</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053534830.1">
        <property type="nucleotide sequence ID" value="XM_053678855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QXF9"/>
    <dbReference type="GeneID" id="128630377"/>
    <dbReference type="KEGG" id="ipu:128630377"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000210">
        <property type="entry name" value="Si:dkey-16p6.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="8">
        <location>
            <begin position="230"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053534830.1"/>
        </source>
    </evidence>
    <sequence length="733" mass="84073" checksum="159128EC3C1D69B4" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QXZ6</accession>
    <name>A0A9F7QXZ6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC128629202</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC128629202</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053532195.1">
        <property type="nucleotide sequence ID" value="XM_053676220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QXZ6"/>
    <dbReference type="GeneID" id="128629202"/>
    <dbReference type="KEGG" id="ipu:128629202"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053532195.1"/>
        </source>
    </evidence>
    <sequence length="254" mass="28457" checksum="583B415F0D752E37" modified="2023-06-28" version="1">METLPERPTPEPRPRSMLTQEEWSRVDATATLRRPRTPHDSSSERVQLNETSTVRRRPKVIAPTQRDDATDAAIRVRPVSEVEHVYRESDMSEDFRRALKPPVSPKPSLGLRKPEPPTPTRRVPLPGPENQHSTVEGKRVPPPVSPKPSPPPTLPKPIKIKPLPTHTLTPPSDLNTHPHSSTILPEQVDHPILSPSTPTPQTSRISHFVPTTFDISRSEPPNPPNSFYPWVRICPSQTPEIFHGRAVSGHSQPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R563</accession>
    <name>A0A9F7R563_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">threonine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Threonyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">tars3</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged tRNA(Thr) via its editing domain, at the post-transfer stage.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="3"/>
    <dbReference type="RefSeq" id="XP_053534135.1">
        <property type="nucleotide sequence ID" value="XM_053678160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R563"/>
    <dbReference type="GeneID" id="108264327"/>
    <dbReference type="KEGG" id="ipu:108264327"/>
    <dbReference type="CTD" id="123283"/>
    <dbReference type="OMA" id="WYADGMY"/>
    <dbReference type="OrthoDB" id="5423599at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01667">
        <property type="entry name" value="TGS_ThrRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00860">
        <property type="entry name" value="ThrRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000009">
        <property type="entry name" value="Threonine--tRNA ligase 2, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000003">
        <property type="entry name" value="Threonine--tRNA ligase 2, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000006">
        <property type="entry name" value="Threonine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000003">
        <property type="entry name" value="Threonine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047246">
        <property type="entry name" value="ThrRS_anticodon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF51">
        <property type="entry name" value="THREONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
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    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Class II aaRS ABD-related"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
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    </dbReference>
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        <property type="entry name" value="TGS-like"/>
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        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 18" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGS" evidence="16">
        <location>
            <begin position="179"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="15">
        <location>
            <begin position="448"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="81"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="2"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="112"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049515"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00058080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_053534135.1"/>
        </source>
    </evidence>
    <sequence length="822" mass="94271" checksum="3A5F873CDE7D6610" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R882</accession>
    <name>A0A9F7R882_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Complement component C7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="31" type="primary">c7a</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="31">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="22">Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis. The MAC is initiated by proteolytic cleavage of C5 into complement C5b in response to the classical, alternative, lectin and GZMK complement pathways. The complement pathways consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. C7 serves as a membrane anchor. During MAC assembly, associates with C5b and C6 to form the C5b-7 complex, a key lipophilic precursor of the MAC complex, which associates with the outer leaflet and reduces the energy for membrane bending.</text>
    </comment>
    <comment type="subunit">
        <text evidence="23">Monomer or dimer; as a C5b-7 complex it can also form multimeric rosettes. Component of the membrane attack complex (MAC), composed of complement C5b, C6, C7, C8A, C8B, C8G and multiple copies of the pore-forming subunit C9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Target cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complement C6/C7/C8/C9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053535297.1">
        <property type="nucleotide sequence ID" value="XM_053679322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R882"/>
    <dbReference type="GeneID" id="108264897"/>
    <dbReference type="KEGG" id="ipu:108264897"/>
    <dbReference type="CTD" id="562475"/>
    <dbReference type="OrthoDB" id="504708at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005579">
        <property type="term" value="C:membrane attack complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044218">
        <property type="term" value="C:other organism cell membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006958">
        <property type="term" value="P:complement activation, classical pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048825">
        <property type="entry name" value="C7_KAZAL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048831">
        <property type="entry name" value="C8A_B_C6_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003884">
        <property type="entry name" value="FacI_MAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040729">
        <property type="entry name" value="Kazal_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001862">
        <property type="entry name" value="MAC_perforin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020863">
        <property type="entry name" value="MACPF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
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        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742">
        <property type="entry name" value="COMPLEMENT COMPONENT C6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742:SF2">
        <property type="entry name" value="COMPLEMENT COMPONENT C7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21195">
        <property type="entry name" value="EGF_C8A_B_C6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18434">
        <property type="entry name" value="Kazal_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21330">
        <property type="entry name" value="Kazal_C7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00764">
        <property type="entry name" value="COMPLEMENTC9"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01705">
        <property type="entry name" value="TSP1REPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00057">
        <property type="entry name" value="FIMAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00279">
        <property type="entry name" value="MACPF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0180">Complement pathway</keyword>
    <keyword evidence="13" id="KW-0204">Cytolysis</keyword>
    <keyword evidence="18 25" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0391">Immunity</keyword>
    <keyword evidence="8" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0473">Membrane attack complex</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="11 27" id="KW-0732">Signal</keyword>
    <keyword evidence="9 25" id="KW-0768">Sushi</keyword>
    <keyword evidence="7" id="KW-1052">Target cell membrane</keyword>
    <keyword evidence="20" id="KW-1053">Target membrane</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="signal peptide" evidence="27">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039944650" description="Complement component C7" evidence="27">
        <location>
            <begin position="25"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="domain" description="MACPF" evidence="29">
        <location>
            <begin position="121"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="28">
        <location>
            <begin position="559"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="28">
        <location>
            <begin position="619"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="503"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="104"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="589"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00073222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00093281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00093478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="RefSeq" id="XP_053535297.1"/>
        </source>
    </evidence>
    <sequence length="837" mass="94804" checksum="FDB0755234BBF8F1" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R9A3</accession>
    <name>A0A9F7R9A3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Protein kinase C</fullName>
            <ecNumber evidence="27">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="39" type="primary">prkcba</name>
    </gene>
    <organism evidence="38 39">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="38" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="38">SDA103</strain>
        </source>
    </reference>
    <reference evidence="39" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="39">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25 27">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="31">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="31">Binds 3 Ca(2+) ions per subunit. The ions are bound to the C2 domain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 27">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="27"/>
    <dbReference type="RefSeq" id="XP_053532378.1">
        <property type="nucleotide sequence ID" value="XM_053676403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R9A3"/>
    <dbReference type="GeneID" id="108258960"/>
    <dbReference type="KEGG" id="ipu:108258960"/>
    <dbReference type="CTD" id="100006675"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20833">
        <property type="entry name" value="C1_cPKC_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20836">
        <property type="entry name" value="C1_cPKC_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04026">
        <property type="entry name" value="C2_PKC_alpha_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05616">
        <property type="entry name" value="STKc_cPKC_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000012">
        <property type="entry name" value="Kinase C alpha type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000023">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000080">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000103">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000006">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000031">
        <property type="entry name" value="Protein kinase C alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034664">
        <property type="entry name" value="cPKC-beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014375">
        <property type="entry name" value="Protein_kinase_C_a/b/g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000550">
        <property type="entry name" value="PKC_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="21" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="9" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="17 27 30" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16 31" id="KW-0106">Calcium</keyword>
    <keyword evidence="18" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="19" id="KW-0391">Immunity</keyword>
    <keyword evidence="14 27 39" id="KW-0418">Kinase</keyword>
    <keyword evidence="22" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 31" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 27 32" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="38" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 27" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="23" id="KW-0804">Transcription</keyword>
    <keyword evidence="20" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8 27" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <keyword evidence="13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="36">
        <location>
            <begin position="32"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="36">
        <location>
            <begin position="97"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="34">
        <location>
            <begin position="154"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="35">
        <location>
            <begin position="338"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="37">
        <location>
            <begin position="597"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="302"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="33">
        <location>
            <begin position="312"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="28">
        <location>
            <position position="462"/>
        </location>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="31">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="30">
        <location>
            <begin position="344"/>
            <end position="352"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="30 32">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00047470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRNR" id="PIRNR000550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PIRSR" id="PIRSR000550-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PIRSR" id="PIRSR000550-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="PIRSR" id="PIRSR000550-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="PIRSR" id="PIRSR000550-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="36">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="37">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="38">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="39">
        <source>
            <dbReference type="RefSeq" id="XP_053532378.1"/>
        </source>
    </evidence>
    <sequence length="668" mass="75961" checksum="FCB6A8A87296AB95" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RCE6</accession>
    <name>A0A9F7RCE6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein LOC128629551</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC128629551</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SDA103</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053534071.1">
        <property type="nucleotide sequence ID" value="XM_053678096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RCE6"/>
    <dbReference type="GeneID" id="128629551"/>
    <dbReference type="KEGG" id="ipu:128629551"/>
    <dbReference type="OrthoDB" id="419189at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46670">
        <property type="entry name" value="ENDO/EXONUCLEASE/PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46670:SF3">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_053534071.1"/>
        </source>
    </evidence>
    <sequence length="316" mass="35168" checksum="61B2EB9A1F0AC99B" modified="2023-06-28" version="1">MHFLVVYRPRGQLGKFIDEFDTPLSTIPDDGTPLLVLGDFNIHLDKPHAADFLALLPTFDLKQFTTPVTHKAGKQLDLILTRNCTTHNLLITPLHISDHFFIQFSTSLPSPPSLSPPSISFHRNLRSLSPSHFSSVVTTLLPSDSHLSSLDLNTATDMLCSTLTSSLDNICPLKSRPARTSPSSPWLSEALRNNWAKLRASERKWRKSNNPTDLTNYQTLLSSFSNSISIAKATFYQEKIGSSPNIRILFKTFSSLLCPPPPPSPTSLTADDFATFFTNKITSIRNQFSTPDMHRPTPPPCNSQLTSFFPLSETDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RML3</accession>
    <name>A0A9F7RML3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Disks large homolog 3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">dlg3</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053538193.1">
        <property type="nucleotide sequence ID" value="XM_053682218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RML3"/>
    <dbReference type="GeneID" id="108268922"/>
    <dbReference type="CTD" id="1741"/>
    <dbReference type="OrthoDB" id="78824at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035255">
        <property type="term" value="F:ionotropic glutamate receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097120">
        <property type="term" value="P:receptor localization to synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099072">
        <property type="term" value="P:regulation of postsynaptic membrane neurotransmitter receptor levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06723">
        <property type="entry name" value="PDZ1_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06724">
        <property type="entry name" value="PDZ2_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06795">
        <property type="entry name" value="PDZ3_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12029">
        <property type="entry name" value="SH3_DLG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000008">
        <property type="entry name" value="Disks large homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000001">
        <property type="entry name" value="Disks large homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000001">
        <property type="entry name" value="Disks large homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000091">
        <property type="entry name" value="disks large homolog 1 isoform X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000027">
        <property type="entry name" value="Disks large homolog 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000002">
        <property type="entry name" value="Disks large homolog 4 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.10">
        <property type="entry name" value="Guanylate Kinase phosphate binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.470">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019583">
        <property type="entry name" value="DLG1-4_PDZ_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016313">
        <property type="entry name" value="DLG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019590">
        <property type="entry name" value="DLG1_PEST_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035763">
        <property type="entry name" value="DLG3_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015143">
        <property type="entry name" value="L27_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036892">
        <property type="entry name" value="L27_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050614">
        <property type="entry name" value="Synaptic_Scaffolding_LAP-MAGUK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119">
        <property type="entry name" value="DISCS LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119:SF28">
        <property type="entry name" value="DISKS LARGE HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09058">
        <property type="entry name" value="L27_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10608">
        <property type="entry name" value="MAGUK_N_PEST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10600">
        <property type="entry name" value="PDZ_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001741">
        <property type="entry name" value="MAGUK_DLGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01277">
        <property type="entry name" value="MAGUK_N_PEST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101288">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51022">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="L27" evidence="12">
        <location>
            <begin position="8"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="11">
        <location>
            <begin position="151"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="11">
        <location>
            <begin position="246"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="11">
        <location>
            <begin position="411"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="9">
        <location>
            <begin position="526"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="10">
        <location>
            <begin position="685"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="647"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="655"/>
            <end position="664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_053538193.1"/>
        </source>
    </evidence>
    <sequence length="881" mass="96655" checksum="CACEEAC7C30D3E91" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RPS3</accession>
    <name>A0A9F7RPS3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="17">LOW QUALITY PROTEIN: zinc finger protein 850</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC108272211</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053539603.1">
        <property type="nucleotide sequence ID" value="XM_053683628.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108272211"/>
    <dbReference type="KEGG" id="ipu:108272211"/>
    <dbReference type="OrthoDB" id="9411774at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002682">
        <property type="term" value="P:regulation of immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001485">
        <property type="entry name" value="Krueppel-related zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002063">
        <property type="entry name" value="RB associated KRAB zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000638">
        <property type="entry name" value="Zinc finger protein 184"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000512">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000212">
        <property type="entry name" value="zinc finger protein 382 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002254">
        <property type="entry name" value="Zinc finger protein 540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001270">
        <property type="entry name" value="zinc finger protein 583 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001188">
        <property type="entry name" value="zinc finger protein 629 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399:SF54">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF26.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="24"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="122"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="150"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="206"/>
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    <sequence length="738" mass="86053" checksum="C7D1B53868C4C30F" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RQX5</accession>
    <name>A0A9F7RQX5_ICTPU</name>
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        <submittedName>
            <fullName evidence="13">Uncharacterized protein LOC108274896</fullName>
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        <name evidence="13" type="primary">LOC108274896</name>
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        <name type="scientific">Ictalurus punctatus</name>
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            <taxon>Eukaryota</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
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                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="13">Blood</tissue>
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            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
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</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Siluriformes</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053540893.1">
        <property type="nucleotide sequence ID" value="XM_053684918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RRK5"/>
    <dbReference type="GeneID" id="128634441"/>
    <dbReference type="KEGG" id="ipu:128634441"/>
    <dbReference type="OrthoDB" id="10072345at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="CDD" id="cd00229">
        <property type="entry name" value="SGNH_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12700">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ester_Hydrolysis_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30383:SF29">
        <property type="entry name" value="SGNH HYDROLASE-TYPE ESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30383">
        <property type="entry name" value="THIOESTERASE 1/PROTEASE 1/LYSOPHOSPHOLIPASE L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGNH hydrolase-type esterase" evidence="2">
        <location>
            <begin position="161"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="17"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053540893.1"/>
        </source>
    </evidence>
    <sequence length="302" mass="33359" checksum="F850DEBE11E879A4" modified="2023-06-28" version="1">MSAVSPVLSADEDSLELHMVRLELEDVEKQIRGLLDKQAQLLERQTALETSCASAHISKVSTQRGISTPSPSTPCVSLCRDRAPRTFPAVVSVTPAPTHLGPWVNQRRKARAGPSPPPVFETPTRNRFAPLRQTRPNTVIVGDSIVRNVRVASSKGKVRTHCFSGACVLDVAAQVSGILKKDERIGAVVLHAGTNDTRLRQTEVLKRDFSSLIETVRGRSPTAKIIVSGPLPTYRRGAEKFSRLLALNDWLVSWCNEQNLVFVNNWNLFWERPRLFRPDGLHPSSLGAELLSDNISKALHSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RSN2</accession>
    <name>A0A9F7RSN2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein si:ch211-241j12.3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">si:ch211-241j12.3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the actin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053542022.1">
        <property type="nucleotide sequence ID" value="XM_053686047.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108255437"/>
    <dbReference type="KEGG" id="ipu:108255437"/>
    <dbReference type="OrthoDB" id="10004999at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd13397">
        <property type="entry name" value="ASKHA_NBD_actin_Arp-T1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000007">
        <property type="entry name" value="Actin like 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000050">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1103"/>
            <end position="1227"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="904"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1112"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1203"/>
            <end position="1212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053542022.1"/>
        </source>
    </evidence>
    <sequence length="1698" mass="189010" checksum="AB02C47162822864" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TGP4</accession>
    <name>A0A9F7TGP4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="1">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC128630102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="1"/>
    <dbReference type="RefSeq" id="XP_053534648.1">
        <property type="nucleotide sequence ID" value="XM_053678673.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TGP4"/>
    <dbReference type="GeneID" id="128630102"/>
    <dbReference type="KEGG" id="ipu:128630102"/>
    <dbReference type="OrthoDB" id="26387at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF28">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053534648.1"/>
        </source>
    </evidence>
    <sequence length="96" mass="10694" checksum="BCB4F9AC374E3ABD" modified="2023-06-28" version="1">MLGAVLQWAMEPSGGHWSESMLQRVLHLIGMALLEEQQQLENIGDDDEVTFNFTLKISRPGEAPGNTPSILALLEALQRAPHLEVHKDMIRHGSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TGR3</accession>
    <name>A0A9F7TGR3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="1">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC108261659</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="1"/>
    <dbReference type="RefSeq" id="XP_053534708.1">
        <property type="nucleotide sequence ID" value="XM_053678733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TGR3"/>
    <dbReference type="GeneID" id="108261659"/>
    <dbReference type="KEGG" id="ipu:108261659"/>
    <dbReference type="OrthoDB" id="26387at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF28">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="294"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053534708.1"/>
        </source>
    </evidence>
    <sequence length="321" mass="36317" checksum="884D4B5C20513EF9" modified="2023-06-28" version="1">MHLQYLMVWCVCIYFLVHKCVLLNICLVLQDFVCACSSNCTAVSFSCKSIFLNYVSFSTQMVVSIKTKRECTAAAPPCEGTGHCHKETVWDKDKAERKRKAELCWKKTMAQTLQSQSHFINKYIDLLTQDSEDLDASASTSAEHSPSSCDGALVCVGPRRWRARGGERRQMEMCILCHEAQEILPDGAVMVLAAFVQRSTVMSENHKRPPHNPESYDPLFMHPDLSFGTHTGSCGHIMHSHCWQRSLNINDANREEKCNRYLHTSQYVCVCVCVCVCVCVCVCICVYMCVYVCICVYMCVYVCICVYMCVYVCICVCLCTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TQ03</accession>
    <name>A0A9F7TQ03_ICTPU</name>
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        <submittedName>
            <fullName evidence="8">SAM domain-containing protein SAMSN-1a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">samsn1a</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053543438.1">
        <property type="nucleotide sequence ID" value="XM_053687463.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="108277652"/>
    <dbReference type="CTD" id="336695"/>
    <dbReference type="OrthoDB" id="10047268at2759"/>
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        <property type="component" value="Chromosome 17"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051725">
        <property type="entry name" value="SAM-SH3_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
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        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021090">
        <property type="entry name" value="SPIDER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12301:SF4">
        <property type="entry name" value="SAM DOMAIN-CONTAINING PROTEIN SAMSN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12301">
        <property type="entry name" value="SAM-DOMAIN, SH3 AND NUCLEAR LOCALIZATION SIGNALS PROTEIN RELATED"/>
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    </dbReference>
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        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
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            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="6">
        <location>
            <begin position="455"/>
            <end position="519"/>
        </location>
    </feature>
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            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="300"/>
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        </location>
    </feature>
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            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
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        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
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        <location>
            <begin position="105"/>
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        </location>
    </feature>
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        <location>
            <begin position="315"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053543438.1"/>
        </source>
    </evidence>
    <sequence length="562" mass="62758" checksum="1823C752DF11C767" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TQ86</accession>
    <name>A0A9F7TQ86_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein LOC108261627 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC108261627</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053541787.1">
        <property type="nucleotide sequence ID" value="XM_053685812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TQ86"/>
    <dbReference type="GeneID" id="108261627"/>
    <dbReference type="KEGG" id="ipu:108261627"/>
    <dbReference type="OrthoDB" id="8960739at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042130">
        <property type="term" value="P:negative regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042102">
        <property type="term" value="P:positive regulation of T cell proliferation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031295">
        <property type="term" value="P:T cell costimulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051713">
        <property type="entry name" value="T-cell_Activation_Regulation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466:SF11">
        <property type="entry name" value="GALECTIN 17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466">
        <property type="entry name" value="T-LYMPHOCYTE ACTIVATION ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="396"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="14">
        <location>
            <begin position="39"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin V-set" evidence="13">
        <location>
            <begin position="49"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="14">
        <location>
            <begin position="156"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin V-set" evidence="13">
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            <begin position="166"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="14">
        <location>
            <begin position="268"/>
            <end position="372"/>
        </location>
    </feature>
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        <location>
            <begin position="427"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="433"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="449"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_053541787.1"/>
        </source>
    </evidence>
    <sequence length="488" mass="53726" checksum="2479D31C26086DC2" modified="2023-06-28" version="1">MMKMFTRSSRHSRGIMTSCYSICIVLGIVCVNPLSAVSEFTVSGQVGSTAVLPCKLQTEVNGPPYIKWSIESETVFERVGEETYQGKGYEGRVDVPEEELRKGNCSLVLRNLTPTDAALYWSAKIVSRTKGSVETETVEISRVHLSVEDPLSAVSEFTVSGHVGSTAVLPCKLQTEVNGPPYIKWSIESVTVFERVGEETYQGEGYEGRVDVPEEELRKGNCSLVLRNLTPTDAALYWSAKIVSRTKGSVETETVEISRVHLSVEVPEFTVSGHVGSTAVLPCELQTEVTGIPHIIWEIKSETVFERRGEETNQGEGYEGRVDVPEEELLKGNCTLVLRNLTPTDAAVYRSYKAVSGTKGSVETKTVEISRVNLSVEEKQKEEREAEKEVPRGVRVGVAGTIAVIAVIAVIGAVLYWCKRRRKQNCRNPDPPQTYSLPNTQINPANTSPDEDPNDQGEDLLNTSQDDHGSVVLEEGAVRYNPVRTDPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TRB2</accession>
    <name>A0A9F7TRB2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11 12">C4b-binding protein alpha chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 11 12 13" type="primary">LOC128635151</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11 12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11 12">Blood</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053542460.1">
        <property type="nucleotide sequence ID" value="XM_053686485.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053542461.1">
        <property type="nucleotide sequence ID" value="XM_053686486.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053542462.1">
        <property type="nucleotide sequence ID" value="XM_053686487.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053542463.1">
        <property type="nucleotide sequence ID" value="XM_053686488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TRB2"/>
    <dbReference type="GeneID" id="128635151"/>
    <dbReference type="KEGG" id="ipu:128635151"/>
    <dbReference type="OrthoDB" id="6480633at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000014">
        <property type="entry name" value="Membrane cofactor protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051277">
        <property type="entry name" value="SEZ6_CSMD_C4BPB_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656">
        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656:SF4">
        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <keyword evidence="1 5" id="KW-0768">Sushi</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044698509" evidence="7">
        <location>
            <begin position="21"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="441"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="8">
        <location>
            <begin position="20"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="8">
        <location>
            <begin position="82"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="8">
        <location>
            <begin position="142"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="8">
        <location>
            <begin position="202"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="8">
        <location>
            <begin position="261"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="8">
        <location>
            <begin position="320"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="8">
        <location>
            <begin position="379"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="84"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="172"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053542460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053542461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053542462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_053542463.1"/>
        </source>
    </evidence>
    <sequence length="489" mass="54714" checksum="2EFA1A8591000BA9" modified="2023-06-28" version="1">MLSYMIICSTLLMTAVTVRAQCERPDVGENRILTEDQQTFPDGSTVTFKCSTGYIPVKSSASRSITCTGSQWSELELQCKKRTCGSPGEIPNGKYIYQPTDGISFGATITAECNEGYMLVGTQTRNCRENGWDGRAPICEVTKCTKPPGITDGTFEPEYDSYDYGHAVIYSCNRGLDLIGLPELTCSSDGTFQPSPPRCLLVSCERPEIPNAVRTEGKSPPYKYNNFVRYRCNKGYRMNGSDYLVCTEDGWNPPPPQCNVITCLKPPDINNGIFNPQKELYEYGETVTWSCNQGFRLGGASTISCTDDGTFQTSPQCLDVICDRPQIEHGYIDSTAEYFVYKSSVRVHCNQGYKMEGSDYLTCEENGWNPPPPRCHLNVICDRPQIENGYIDSTEKYFVYKSSVRVHCNQGYKMEGSDYLTCGENGWNPPPPQCHLSPVPWIRIILIVLGVLVCALVCVGCYCWKKKKHIKTIQLDSKPEDDSLVLHHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TSU0</accession>
    <name>A0A9F7TSU0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC128635404</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="2"/>
    <dbReference type="RefSeq" id="XP_053544249.1">
        <property type="nucleotide sequence ID" value="XM_053688274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TSU0"/>
    <dbReference type="GeneID" id="128635404"/>
    <dbReference type="KEGG" id="ipu:128635404"/>
    <dbReference type="OrthoDB" id="8962756at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004868">
        <property type="entry name" value="DNA-dir_DNA_pol_B_mt/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33568">
        <property type="entry name" value="DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33568:SF3">
        <property type="entry name" value="DNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03175">
        <property type="entry name" value="DNA_pol_B_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B mitochondria/virus" evidence="9">
        <location>
            <begin position="63"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B mitochondria/virus" evidence="9">
        <location>
            <begin position="253"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053544249.1"/>
        </source>
    </evidence>
    <sequence length="506" mass="57838" checksum="C16BCC4E4F362332" modified="2023-06-28" version="1">MFLGNRLNSSQQQFDDKVEILEKAYGLDVEVMWECDWNNAKQNDVAVMAFMSTYIHPERLKPRNALFGGRTNAYKLYHKADNSEKIRYVDFTSLYPFCQAKKCYPIGHPQIIFKDFEPLENYYGFVKATVLPPRKLLHPVLPYRTGGKLMFPLCRTCAEHQNQTDPCGHTDEERAISGCWVSIELLKGVEKGYIVTNVDEVWHFPQRSETLFCDYVKTFLQYKQEASGFPAHVMTDADKESYISDYFEKEGIKMDAGKISRNPARRSINKLLLNSLWGRFSMRENLPCTELISDPEQFTQHIFGDGYDVKHFSFVSDSVALIQWCYGDGKGGQTRDINIFLGAFTTAHARLELYELMDKLGDRLLYSDTDSVIFTSREGDWEPPLGPYLGNLTDEVGAGDHIVEFCSGGPKTYGYRTAKGKVCMKAKGVTLNAVNSKAIRLDTLIGLVDHYVVGEDNSRHILAYTDTIVRNKKQFTLHNRSVVKKFKVVYNKRVLLPDFTTIPYGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3TFE2</accession>
    <name>E3TFE2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7 9">Cholesterol 25-hydroxylase-like protein</fullName>
            <ecNumber evidence="9">1.14.99.38</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CH25H</name>
        <name evidence="9" type="synonym">ch25h</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.14.99.38" evidence="9"/>
    <dbReference type="EMBL" id="GU589076">
        <property type="protein sequence ID" value="ADO29028.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001188084.1">
        <property type="nucleotide sequence ID" value="NM_001201155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TFE2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005282"/>
    <dbReference type="GeneID" id="100528885"/>
    <dbReference type="KEGG" id="ipu:100528885"/>
    <dbReference type="CTD" id="9023"/>
    <dbReference type="OrthoDB" id="1658724at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001567">
        <property type="term" value="F:cholesterol 25-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="6">
        <location>
            <begin position="132"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADO29028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="NP_001188084.1"/>
        </source>
    </evidence>
    <sequence length="270" mass="32010" checksum="D21FBCBA28C4444D" modified="2011-01-11" version="1">MSQLTQDQSNISQLTQAQSNMSQLQGIWNCILLYETWLRPPLFPVLFSLAVYLSFCLPFVLLDLLSSRVDLVRRYKIQQKPVTLRMMWNCLALSLYNHVVYIFPLSVLHWYWRPVIYPAEAPRVLEVIGHLVSCLLLFDFQYFIWHVLHHKVPWLYRTFHKVHHKHTSTFVLTTEYSGAWETLSLGLFAAMNPMLLDCHPFTEMLFHILNMWLSVEDHCRYDLPWATHRLVPFGLYGGAPHHDLHHQKFKANYAPYFTHWDKLFGTLHSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3TFS1</accession>
    <name>E3TFS1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative hydrolase rbbp9</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="3">Serine hydrolase RBBP9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">RBBP9</name>
        <name evidence="3" type="synonym">rbbp9</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="GU589205">
        <property type="protein sequence ID" value="ADO29157.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187815.1">
        <property type="nucleotide sequence ID" value="NM_001200886.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TFS1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018114"/>
    <dbReference type="GeneID" id="100528616"/>
    <dbReference type="KEGG" id="ipu:100528616"/>
    <dbReference type="CTD" id="10741"/>
    <dbReference type="OrthoDB" id="2369073at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010662">
        <property type="entry name" value="RBBP9/YdeN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15394">
        <property type="entry name" value="SERINE HYDROLASE RBBP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15394:SF3">
        <property type="entry name" value="SERINE HYDROLASE RBBP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06821">
        <property type="entry name" value="Ser_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADO29157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="NP_001187815.1"/>
        </source>
    </evidence>
    <sequence length="184" mass="20969" checksum="A163D3E1365FD479" modified="2011-01-11" version="1">MAEKRVVIVPGNGSGDVEHSNWYGWVYKQINQISGVCCELRNMPDPVTARESIWLPFMEKELRCDEETLIIGHSSGAAAAMRYSETHKVFGIILVGAYTSDLGDENERKSGYFNRPWEWERIRANVKHIIQFGSTDDPFLPWDEQQEVADGLEVELHKYTDRGHFQNTHFPELISAAQKLITAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3TGD4</accession>
    <accession>A0A979FCX1</accession>
    <name>E3TGD4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3 5 6">LYR motif-containing protein 5A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LYM5A</name>
        <name evidence="5 6" type="synonym">etfrf1</name>
        <name evidence="5 6" type="synonym">lym5a</name>
        <name evidence="5 6" type="synonym">lyrm5a</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2016" name="Biol. Reprod." volume="95" first="19" last="19">
            <title>Transcriptome Display During Testicular Differentiation of Channel Catfish (Ictalurus punctatus) as Revealed by RNA-Seq Analysis.</title>
            <authorList>
                <person name="Zeng Q."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Zhang Y."/>
                <person name="Yuan Z."/>
                <person name="Jiang C."/>
                <person name="Chen A."/>
                <person name="Fu Q."/>
                <person name="Su B."/>
                <person name="Dunham R."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="27307075"/>
            <dbReference type="DOI" id="10.1095/biolreprod.116.138818"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I LYR family.</text>
    </comment>
    <dbReference type="EMBL" id="GU589420">
        <property type="protein sequence ID" value="ADO29370.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187967.1">
        <property type="nucleotide sequence ID" value="NM_001201038.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047018025.2">
        <property type="nucleotide sequence ID" value="XM_047162069.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TGD4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020039"/>
    <dbReference type="GeneID" id="100528768"/>
    <dbReference type="KEGG" id="ipu:100528768"/>
    <dbReference type="CTD" id="553589"/>
    <dbReference type="GeneTree" id="ENSGT00390000001810"/>
    <dbReference type="OMA" id="HRAFMSK"/>
    <dbReference type="OrthoDB" id="10258445at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090324">
        <property type="term" value="P:negative regulation of oxidative phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:respiratory electron transport chain"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Complex1_LYR_dom"/>
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        <property type="entry name" value="Complex1_LYR_ETFRF1_LYRM5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052000">
        <property type="entry name" value="ETFRF1"/>
    </dbReference>
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        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN REGULATORY FACTOR 1"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR21024">
        <property type="entry name" value="GROWTH HORMONE-INDUCIBLE SOLUBLE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Complex1_LYR"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="8"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADO29370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="NP_001187967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_047018025.2"/>
        </source>
    </evidence>
    <sequence length="85" mass="10441" checksum="21CE64B1BAEEFA3C" modified="2011-01-11" version="1">MSNPLRGEVIRLYKNLLYLGREYPKGSVYFRERLKSAFLKNRDVTDPEKIQKLIHRGEFVIKELEALYFLRKYRAMKKRYYEPEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3TGN4</accession>
    <name>E3TGN4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3 5">death domain-containing protein CRADD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CRADD</name>
        <name evidence="5" type="synonym">cradd</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="GU589520">
        <property type="protein sequence ID" value="ADO29470.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001188242.1">
        <property type="nucleotide sequence ID" value="NM_001201313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TGN4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000015391"/>
    <dbReference type="GeneID" id="100529043"/>
    <dbReference type="KEGG" id="ipu:100529043"/>
    <dbReference type="CTD" id="8738"/>
    <dbReference type="GeneTree" id="ENSGT00390000014448"/>
    <dbReference type="OrthoDB" id="10031931at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070513">
        <property type="term" value="F:death domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08327">
        <property type="entry name" value="CARD_RAIDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08319">
        <property type="entry name" value="Death_RAIDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
        <property type="entry name" value="CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042148">
        <property type="entry name" value="CARD_RAIDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037939">
        <property type="entry name" value="CRADD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037926">
        <property type="entry name" value="CRADD_Death"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15034">
        <property type="entry name" value="DEATH DOMAIN-CONTAINING PROTEIN CRADD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15034:SF5">
        <property type="entry name" value="DEATH DOMAIN-CONTAINING PROTEIN CRADD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00619">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00114">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="domain" description="Death" evidence="1">
        <location>
            <begin position="123"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADO29470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="NP_001188242.1"/>
        </source>
    </evidence>
    <sequence length="206" mass="23520" checksum="58D15C0D568742B6" modified="2011-01-11" version="1">MEPKHRDLLRAQRLHLCEQLVVDETIVQYLYQEDILTEGQVEEIQSQKSNKNKTLLLLSILPNRGPNAFNVFVQSLEQDFPWIKEKLLLLADEDEQDGGRGTELSNTTAQWAIPKDILQMVPSDQQLNRLAARLGLEWEMVLLDLGLTSVEITRCRADHPHSSHSQVLAALVMWKQTQGRSATVQRLLQSLQYSEIHPSSLSQVFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-02-19" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>V5RLP3</accession>
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        <submittedName>
            <fullName evidence="6">Transcription factor p65</fullName>
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    <gene>
        <name evidence="6" type="primary">rela</name>
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    <organism evidence="4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2013-08" db="EMBL/GenBank/DDBJ databases">
            <title>Transcription Factor Expression and Regulation in Innate Immunity of Channel Catfish (Ictalurus punctatus).</title>
            <authorList>
                <person name="Leary J.H.III."/>
                <person name="Jaso-Friedmann L."/>
                <person name="Evans D.L."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="journal article" date="2016" name="Biol. Reprod." volume="95" first="19" last="19">
            <title>Transcriptome Display During Testicular Differentiation of Channel Catfish (Ictalurus punctatus) as Revealed by RNA-Seq Analysis.</title>
            <authorList>
                <person name="Zeng Q."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Zhang Y."/>
                <person name="Yuan Z."/>
                <person name="Jiang C."/>
                <person name="Chen A."/>
                <person name="Fu Q."/>
                <person name="Su B."/>
                <person name="Dunham R."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="27307075"/>
            <dbReference type="DOI" id="10.1095/biolreprod.116.138818"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="5">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="component" value="Chromosome 29"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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    <feature type="region of interest" description="Disordered" evidence="2">
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        <source>
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        <source>
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        <source>
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            <fullName evidence="16 18">Interleukin-6 receptor subunit alpha</fullName>
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        <name type="scientific">Ictalurus punctatus</name>
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        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
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                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
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            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="16">Mixed</tissue>
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    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
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    <reference evidence="18" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
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    <comment type="similarity">
        <text evidence="2">Belongs to the type I cytokine receptor family. Type 3 subfamily.</text>
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        <property type="component" value="Chromosome 1"/>
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        <property type="entry name" value="Hematopoietin_rcpt_L_F3_CS"/>
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        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="TypeI_recpt_CBD"/>
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        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CYTOKINE RECEPTOR COMMON SUBUNIT BETA"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="IL6Ra-bind"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 16 18" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540161" evidence="13">
        <location>
            <begin position="20"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="500"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="207"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="359"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AHH39193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017324414.1"/>
        </source>
    </evidence>
    <sequence length="588" mass="65711" checksum="4A8E236F8E8A9484" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5UBA9</accession>
    <name>W5UBA9_ICTPU</name>
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        <recommendedName>
            <fullName evidence="6">Purine-rich element-binding protein gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PURG</name>
        <name evidence="10" type="synonym">purg</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PUR DNA-binding protein family.</text>
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        <property type="protein sequence ID" value="AHH38859.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_017488736.3"/>
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    <dbReference type="KEGG" id="ipu:108276783"/>
    <dbReference type="CTD" id="29942"/>
    <dbReference type="GeneTree" id="ENSGT00950000183162"/>
    <dbReference type="OMA" id="EHSNNGQ"/>
    <dbReference type="OrthoDB" id="523901at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032422">
        <property type="term" value="F:purine-rich negative regulatory element binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Purine-rich element binding protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2450.30:FF:000002">
        <property type="entry name" value="purine-rich element-binding protein gamma isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2450.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006628">
        <property type="entry name" value="PUR-bd_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12611">
        <property type="entry name" value="PUR-TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12611:SF3">
        <property type="entry name" value="PURINE-RICH ELEMENT-BINDING PROTEIN GAMMA"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF04845">
        <property type="entry name" value="PurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00712">
        <property type="entry name" value="PUR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="119"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="141"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00072284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AHH38859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017344225.1"/>
        </source>
    </evidence>
    <sequence length="336" mass="38348" checksum="AEE9A8D54E6A6F96" modified="2014-04-16" version="1">MADGNTREMERGKGRITGDSFPRGGYAQQYSTALPALQGPDIQELASKRVDIQKKRFYLDVKQSARGRFLKIAEVWIGRGRHDSVRKSKLTLSMSMAPDLRYCLGDFIDYYAHVGLRGGHAPRPEEPSDAQRRRQPDHHAAPASPSGSAASEEPAHRVLKSESIEGDNRKYYLDLKENQRGRFLRIRQTVSRGQGAVGYYGQAVEQTIVLPAQGLIEFRDALSQLIEDYGDEREANGRTGRGERDREAACAELPEAASFRVDNKRFYFDVGSNRYGVFLKISEVRQPYRNTITVPLKAWARFGENFIRYEEEMRRLFTCRKEKRTEAQADSEERED</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5UCB1</accession>
    <name>W5UCB1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Single-stranded DNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SSBP1</name>
        <name evidence="11" type="synonym">ssbp1</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
            <location evidence="1">Mitochondrion nucleoid</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT411774">
        <property type="protein sequence ID" value="AHH39598.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017350360.1">
        <property type="nucleotide sequence ID" value="XM_017494871.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UCB1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000023738"/>
    <dbReference type="GeneID" id="108280094"/>
    <dbReference type="KEGG" id="ipu:108280094"/>
    <dbReference type="CTD" id="6742"/>
    <dbReference type="GeneTree" id="ENSGT00390000002796"/>
    <dbReference type="OMA" id="IKSRQWQ"/>
    <dbReference type="OrthoDB" id="1078367at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006264">
        <property type="term" value="P:mitochondrial DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04496">
        <property type="entry name" value="SSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000129">
        <property type="entry name" value="Single-stranded DNA-binding protein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00984">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000424">
        <property type="entry name" value="Primosome_PriB/ssb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011344">
        <property type="entry name" value="ssDNA-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00621">
        <property type="entry name" value="ssb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10302">
        <property type="entry name" value="SINGLE-STRANDED DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10302:SF0">
        <property type="entry name" value="SINGLE-STRANDED DNA-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00436">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002070">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50935">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1135">Mitochondrion nucleoid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR002070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AHH39598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017350360.1"/>
        </source>
    </evidence>
    <sequence length="146" mass="16686" checksum="E4BEBCAB2EBE566E" modified="2014-04-16" version="1">MLRSGSALLFRQIVRNRSTDASLILERSINRVQLLGRVGQDPVMRQVEGRNPVTIFSMATNEMWRSGEGEIPMTGDVSQKTTWHRISVFKPGLRDVAYNYVKKGSRILVEGKLDYGEYVDKNNVRRQATTIIADNIVFLSDIRDRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5UCI5</accession>
    <name>W5UCI5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3 5 6">U7 snRNA-associated Sm-like protein LSm10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">Lsm10</name>
        <name evidence="5 6 7" type="synonym">lsm10</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5 6 7" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5 6 7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT411788">
        <property type="protein sequence ID" value="AHH39605.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017340073.1">
        <property type="nucleotide sequence ID" value="XM_017484584.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017340074.1">
        <property type="nucleotide sequence ID" value="XM_017484585.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047015926.1">
        <property type="nucleotide sequence ID" value="XM_047159970.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UCI5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008452"/>
    <dbReference type="GeneID" id="108274422"/>
    <dbReference type="KEGG" id="ipu:108274422"/>
    <dbReference type="CTD" id="84967"/>
    <dbReference type="GeneTree" id="ENSGT00510000048364"/>
    <dbReference type="OrthoDB" id="10256176at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071254">
        <property type="term" value="C:cytoplasmic U snRNP body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071208">
        <property type="term" value="F:histone pre-mRNA DCP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071209">
        <property type="term" value="F:U7 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006398">
        <property type="term" value="P:mRNA 3'-end processing by stem-loop binding and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01733">
        <property type="entry name" value="LSm10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052840">
        <property type="entry name" value="U7_snRNA_Sm-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21196">
        <property type="entry name" value="U7 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21196:SF1">
        <property type="entry name" value="U7 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="2">
        <location>
            <begin position="15"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AHH39605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017340073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017340074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_047015926.1"/>
        </source>
    </evidence>
    <sequence length="138" mass="15732" checksum="1ECBAEE2DEE5B9C4" modified="2014-04-16" version="1">MATQSLAERTISENSLVVLLQGLHGQVTTVELRDESTARGRVLNVDAFMNVRLEDVLYRDRRGNVSEMADLFVTGRNVRYVHIPDHVDIVDTIQTQLEKIHRVRCFTEKGRKEYSKKKKGFPQSAHAASETHTQAQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5UDJ2</accession>
    <name>W5UDJ2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serine/threonine protein phosphatase 2A regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Ppp2r5a</name>
        <name evidence="6" type="synonym">ppp2r5a</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphatase 2A regulatory subunit B56 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT406814">
        <property type="protein sequence ID" value="AHH37732.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017311473.1">
        <property type="nucleotide sequence ID" value="XM_017455984.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UDJ2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032471"/>
    <dbReference type="GeneID" id="108257875"/>
    <dbReference type="KEGG" id="ipu:108257875"/>
    <dbReference type="CTD" id="5525"/>
    <dbReference type="OMA" id="RCMSSSH"/>
    <dbReference type="OrthoDB" id="10264446at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072542">
        <property type="term" value="F:protein phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000010">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
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        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE 2A PP2A REGULATORY SUBUNIT B"/>
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    </dbReference>
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        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A 56 KDA REGULATORY SUBUNIT ALPHA ISOFORM"/>
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    </dbReference>
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            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017311473.1"/>
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</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5UE26</accession>
    <name>W5UE26_ICTPU</name>
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        <submittedName>
            <fullName evidence="11 13">Zinc finger MIZ domain-containing protein 1</fullName>
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    <gene>
        <name evidence="11" type="primary">ZMIZ1</name>
        <name evidence="13" type="synonym">zmiz1a</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <tissue evidence="13">Blood</tissue>
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    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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        <property type="molecule type" value="mRNA"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Zinc finger MIZ domain-containing protein 2"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF7">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25527">
        <property type="entry name" value="GBD-like_ZMIZ1_ZMIZ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18028">
        <property type="entry name" value="Zmiz1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
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    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SP-RING-type" evidence="10">
        <location>
            <begin position="727"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="115"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="197"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="331"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="865"/>
            <end position="1068"/>
        </location>
    </feature>
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        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
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            <begin position="243"/>
            <end position="255"/>
        </location>
    </feature>
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            <begin position="345"/>
            <end position="355"/>
        </location>
    </feature>
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            <end position="440"/>
        </location>
    </feature>
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        <location>
            <begin position="480"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
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            <end position="521"/>
        </location>
    </feature>
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            <end position="895"/>
        </location>
    </feature>
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        <location>
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            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1024"/>
            <end position="1068"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AHH40218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017319779.1"/>
        </source>
    </evidence>
    <sequence length="1068" mass="114908" checksum="55BC5C4258DCAA82" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5UHF8</accession>
    <name>W5UHF8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14 16">Homeobox protein Hox-C10a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14 16" type="primary">hoxc10a</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Abd-B homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="JT410545">
        <property type="protein sequence ID" value="AHH39107.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017343130.1">
        <property type="nucleotide sequence ID" value="XM_017487641.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UHF8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033297"/>
    <dbReference type="GeneID" id="108276182"/>
    <dbReference type="KEGG" id="ipu:108276182"/>
    <dbReference type="CTD" id="100535576"/>
    <dbReference type="GeneTree" id="ENSGT00940000160855"/>
    <dbReference type="OMA" id="RLGDNSC"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000018">
        <property type="entry name" value="Homeobox A10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046333">
        <property type="entry name" value="HXA10/ABDB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45874">
        <property type="entry name" value="HOMEOBOX PROTEIN ABDOMINAL-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45874:SF2">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-C10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="263"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="265"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="126"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="193"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="215"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AHH39107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017343130.1"/>
        </source>
    </evidence>
    <sequence length="338" mass="38241" checksum="87714C32042E4130" modified="2014-04-16" version="1">MSCPNNVAAGSFLMDSLVGETSPYRGEGYSANSGMYISTSAEYGCQMMRNLGVAGMPHSKRDELPPSTVLISGYEHAHYLSQRDAWTTASKTYRGAQPVAQPLHPCSFTSSGVKEEAITCLYQSDIDSPRESTDSSTYIRLGDNSNRTDQSGVSSAGYFRGNQVYTTERVHGEDGFDSDFSRISRITTPTETRTVDSFVKSSKSQQDASGEETNAEEHRVDKRQQLRKEDGGPKNDSCTDDSESELKDEAKLENTTGNWLKAKSGRKKRCPYTKHQTLELEKEFLFNMYLTRERRLEISKSINLTDRQVKIWFQNRRMKLKKLNRENRVRDLTGYNFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>W5UK44</accession>
    <accession>A0A2D0Q373</accession>
    <name>W5UK44_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Galectin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LGALS8</name>
        <name evidence="7" type="synonym">lgals8a</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT412922">
        <property type="protein sequence ID" value="AHH40047.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017311810.2">
        <property type="nucleotide sequence ID" value="XM_017456321.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UK44"/>
    <dbReference type="SMR" id="W5UK44"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008997"/>
    <dbReference type="GeneID" id="108258055"/>
    <dbReference type="KEGG" id="ipu:108258055"/>
    <dbReference type="CTD" id="563573"/>
    <dbReference type="GeneTree" id="ENSGT00940000155727"/>
    <dbReference type="OrthoDB" id="6251307at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00070">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000023">
        <property type="entry name" value="Galectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000124">
        <property type="entry name" value="Galectin-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044156">
        <property type="entry name" value="Galectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001079">
        <property type="entry name" value="Galectin_CRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346:SF111">
        <property type="entry name" value="GALECTIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00337">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00908">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00276">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51304">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1 3" id="KW-0430">Lectin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Galectin" evidence="4">
        <location>
            <begin position="17"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Galectin" evidence="4">
        <location>
            <begin position="182"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU102079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AHH40047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017311810.2"/>
        </source>
    </evidence>
    <sequence length="312" mass="34753" checksum="5448AC866A4E85EF" modified="2014-04-16" version="1">MSVKQPKQIVLKPHIPIAVTILGGLHPGEMVLIQGSVPADADRFQVDFTCGSSSKPRADIAFHLNPRFKKDHIVCNTLQLEYWGKEEIHYQMPFRKGADFEIIVLVEKDLYKVAVNGAHVLEYKHRIDLARVDTLCVSGKVQIQAIGFIPSTSTSGSPVAVKGISTQAPVVVDDSCDMSLPFRRKLIKGLSPGNTITVKGQIIAYANSFSVNLRVGNSEDIALHLNPRLRSGLFIRNSYLSECWGPEENTLPSFPFTPGEYFEMIILCEAQQFKVAVNGAHQLTYKHRVQDLSRVDELELTGDLELLDVKIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5UK93</accession>
    <name>W5UK93_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3 5">Protein phosphatase 1H</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">ppm1h</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT413042">
        <property type="protein sequence ID" value="AHH40092.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017341179.1">
        <property type="nucleotide sequence ID" value="XM_017485690.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UK93"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018156"/>
    <dbReference type="GeneID" id="108275097"/>
    <dbReference type="KEGG" id="ipu:108275097"/>
    <dbReference type="CTD" id="57460"/>
    <dbReference type="GeneTree" id="ENSGT00940000165017"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004741">
        <property type="term" value="F:[pyruvate dehydrogenase (acetyl-transferring)]-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF838">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="131"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="209"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AHH40092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017341179.1"/>
        </source>
    </evidence>
    <sequence length="528" mass="58336" checksum="305C667002AD6E4C" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5UKR1</accession>
    <name>W5UKR1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Arylsulfatase K</fullName>
            <ecNumber evidence="12">3.1.6.1</ecNumber>
            <ecNumber evidence="14">3.1.6.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Glucuronate-2-sulfatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21 23" type="primary">arsk</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Catalyzes the hydrolysis of pseudosubstrates such as p-nitrocatechol sulfate and p-nitrophenyl sulfate. Catalyzes the hydrolysis of the 2-sulfate groups of the 2-O-sulfo-D-glucuronate residues of chondroitin sulfate, heparin and heparitin sulfate. Acts selectively on 2-sulfoglucuronate and lacks activity against 2-sulfoiduronate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Hydrolysis of the 2-sulfate groups of the 2-O-sulfo-D-glucuronate residues of chondroitin sulfate, heparin and heparitin sulfate.</text>
            <dbReference type="EC" id="3.1.6.18"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>an aryl sulfate + H2O = a phenol + sulfate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17261"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:33853"/>
            <dbReference type="ChEBI" id="CHEBI:140317"/>
            <dbReference type="EC" id="3.1.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sulfatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.6.1" evidence="12"/>
    <dbReference type="EC" id="3.1.6.18" evidence="14"/>
    <dbReference type="EMBL" id="JT417179">
        <property type="protein sequence ID" value="AHH42285.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017306938.1">
        <property type="nucleotide sequence ID" value="XM_017451449.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UKR1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027071"/>
    <dbReference type="GeneID" id="108255492"/>
    <dbReference type="KEGG" id="ipu:108255492"/>
    <dbReference type="CTD" id="153642"/>
    <dbReference type="OMA" id="RAYFGAC"/>
    <dbReference type="OrthoDB" id="1886626at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004065">
        <property type="term" value="F:arylsulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015024">
        <property type="term" value="F:glucuronate-2-sulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000039">
        <property type="entry name" value="arylsulfatase K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051849">
        <property type="entry name" value="GAG-degrading_sulfatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46615">
        <property type="entry name" value="ARYLSULFATASE K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46615:SF1">
        <property type="entry name" value="ARYLSULFATASE K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0458">Lysosome</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 19" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540343" description="Arylsulfatase K" evidence="19">
        <location>
            <begin position="27"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase N-terminal" evidence="20">
        <location>
            <begin position="29"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00035026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00035590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00035675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00035710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00035719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="AHH42285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017306938.1"/>
        </source>
    </evidence>
    <sequence length="524" mass="59453" checksum="A6B478D1CABB1668" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-06-07" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1V0JI28</accession>
    <name>A0A1V0JI28_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">C-C motif chemokine 18</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="8">Chemokine (C-C motif) ligand 36, duplicate 13 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CCL36.13</name>
        <name evidence="10" type="synonym">LOC108267255</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2017" name="Dev. Comp. Immunol." volume="73" first="97" last="108">
            <title>The chemokinome superfamily: II. The 64 CC chemokines in channel catfish and their involvement in disease and hypoxia responses.</title>
            <authorList>
                <person name="Fu Q."/>
                <person name="Yang Y."/>
                <person name="Li C."/>
                <person name="Zeng Q."/>
                <person name="Zhou T."/>
                <person name="Li N."/>
                <person name="Liu Y."/>
                <person name="Li Y."/>
                <person name="Wang X."/>
                <person name="Liu S."/>
                <person name="Li D."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="28322933"/>
            <dbReference type="DOI" id="10.1016/j.dci.2017.03.012"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the intercrine beta (chemokine CC) family.</text>
    </comment>
    <dbReference type="EMBL" id="KY421930">
        <property type="protein sequence ID" value="ARD08891.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053537195.1">
        <property type="nucleotide sequence ID" value="XM_053681220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1V0JI28"/>
    <dbReference type="KEGG" id="ipu:108267255"/>
    <dbReference type="OrthoDB" id="8934837at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00272">
        <property type="entry name" value="Chemokine_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000002">
        <property type="entry name" value="C-C motif chemokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039809">
        <property type="entry name" value="Chemokine_b/g/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015:SF183">
        <property type="entry name" value="C-C MOTIF CHEMOKINE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015">
        <property type="entry name" value="SMALL INDUCIBLE CYTOKINE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00199">
        <property type="entry name" value="SCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044566622" evidence="6">
        <location>
            <begin position="21"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="7">
        <location>
            <begin position="26"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ARD08891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053537195.1"/>
        </source>
    </evidence>
    <sequence length="93" mass="10363" checksum="6017402F0D161196" modified="2017-06-07" version="1">MFFRCLLLVLVCLQDFTTDADTANGASLCCFEFHKKPFPAANVVSYEETRSDCMLPGVILTTKKGYRICADPEVDWVKQIIANKNLAKTPATN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PK51</accession>
    <name>A0A2D0PK51_ICTPU</name>
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        <submittedName>
            <fullName evidence="5">SEC14-like lipid binding 8</fullName>
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        <name evidence="5" type="primary">sec14l8</name>
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    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017307003.1">
        <property type="nucleotide sequence ID" value="XM_017451514.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A2D0PK51"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030317"/>
    <dbReference type="GeneID" id="108255515"/>
    <dbReference type="KEGG" id="ipu:108255515"/>
    <dbReference type="CTD" id="558964"/>
    <dbReference type="OMA" id="RWQQQLF"/>
    <dbReference type="OrthoDB" id="1434354at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000009">
        <property type="entry name" value="SEC14-like 2 (S. cerevisiae)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GOLD domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
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        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
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        <property type="entry name" value="GOLD_dom"/>
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        <property type="entry name" value="GOLD_dom_sf"/>
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    <dbReference type="InterPro" id="IPR051064">
        <property type="entry name" value="SEC14/CRAL-TRIO_domain"/>
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    <dbReference type="PANTHER" id="PTHR23324">
        <property type="entry name" value="SEC14 RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23324:SF83">
        <property type="entry name" value="SEC14-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
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        <property type="entry name" value="CRETINALDHBP"/>
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        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
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        <property type="match status" value="1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="77"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="3">
        <location>
            <begin position="276"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="390"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="390"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017307003.1"/>
        </source>
    </evidence>
    <sequence length="409" mass="46666" checksum="35182274B915E56E" modified="2017-12-20" version="1">MSGRVGDLSVKQAEALAQFRERVQDVLPQCPSQSDHFLLRWLRARNFNLQKAEAMLRKHLEFRKQMKADTITTDWKPPEVLEKFFSGGMCGYDREGSPVWYDVIGPVDPKGLLLSASKQDFMKTKVRDCEILQKECSLQSERLGKNVESITMIYDCEGLGLKHLWKPAIETYGEVLTMFEDNYPEGLKRLLIIKAPKLFPVAYNLVKHFLSEDTRHKIMVLGSNWQEVLKKLIDPEELPAMYGGKLTDPDGDPRCRTRIKQGGLVPQSYYARDFIKMEYDQCVSISRGSSHQLEYEVLAPGCVLRWQFCCDGGDVGFGVFMKKKLGEWMKASQMQEVLVSQRYNAHLVPEDGSLTCTEPGVYVLRFDNTYSIFQSKKVSFSVEVLLLSNDSQSQPETSRTPGRDVPSDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PLR5</accession>
    <name>A0A2D0PLR5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">DnaJ homolog subfamily C member 16</fullName>
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    </protein>
    <gene>
        <name evidence="6 7" type="primary">dnajc16l</name>
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    <organism evidence="5 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6 7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017306360.1">
        <property type="nucleotide sequence ID" value="XM_017450871.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017306361.1">
        <property type="nucleotide sequence ID" value="XM_017450872.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PLR5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035661"/>
    <dbReference type="GeneID" id="108255153"/>
    <dbReference type="KEGG" id="ipu:108255153"/>
    <dbReference type="CTD" id="556590"/>
    <dbReference type="OMA" id="QPEFAST"/>
    <dbReference type="OrthoDB" id="10065037at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02963">
        <property type="entry name" value="TRX_DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052448">
        <property type="entry name" value="DnaJ_C16_autophagy_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043361">
        <property type="entry name" value="DNAJC16_TRX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44303">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44303:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013510445" evidence="3">
        <location>
            <begin position="28"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="528"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="4">
        <location>
            <begin position="31"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="558"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="569"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017306360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017306361.1"/>
        </source>
    </evidence>
    <sequence length="761" mass="87709" checksum="FC7DC34C850BEBE7" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PQ05</accession>
    <name>A0A2D0PQ05_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Neurogenic locus notch homolog protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="35" type="primary">notch1b</name>
    </gene>
    <organism evidence="34 35">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="34">SDA103</strain>
        </source>
    </reference>
    <reference evidence="35" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="35">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NOTCH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="29">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017307516.1">
        <property type="nucleotide sequence ID" value="XM_017452027.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000021852"/>
    <dbReference type="GeneID" id="108255797"/>
    <dbReference type="KEGG" id="ipu:108255797"/>
    <dbReference type="CTD" id="794892"/>
    <dbReference type="OMA" id="FICTCTP"/>
    <dbReference type="OrthoDB" id="283575at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009792">
        <property type="term" value="P:embryo development ending in birth or egg hatching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048568">
        <property type="term" value="P:embryonic organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060429">
        <property type="term" value="P:epithelium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031017">
        <property type="term" value="P:exocrine pancreas development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010001">
        <property type="term" value="P:glial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060218">
        <property type="term" value="P:hematopoietic stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050768">
        <property type="term" value="P:negative regulation of neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007422">
        <property type="term" value="P:peripheral nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045597">
        <property type="term" value="P:positive regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042659">
        <property type="term" value="P:regulation of cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901222">
        <property type="term" value="P:regulation of non-canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035282">
        <property type="term" value="P:segmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="28"/>
    </dbReference>
    <dbReference type="CDD" id="cd21702">
        <property type="entry name" value="JMTM_Notch1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000151">
        <property type="entry name" value="FAT atypical cadherin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000005">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000004">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000080">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000136">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000279">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.320:FF:000001">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000031">
        <property type="entry name" value="neurogenic locus notch homolog protein 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000100">
        <property type="entry name" value="neurogenic locus notch homolog protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000327">
        <property type="entry name" value="neurogenic locus notch homolog protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000060">
        <property type="entry name" value="Neurogenic locus notch protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000092">
        <property type="entry name" value="Neurogenic locus notch protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000127">
        <property type="entry name" value="Neurogenic locus notch protein 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000157">
        <property type="entry name" value="Neurogenic locus notch protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000253">
        <property type="entry name" value="Neurogenic locus notch protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000524">
        <property type="entry name" value="Neurogenic locus notch protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000125">
        <property type="entry name" value="Neurogenic locus notch protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000095">
        <property type="entry name" value="Notch, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000146">
        <property type="entry name" value="Putative neurogenic locus notch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000373">
        <property type="entry name" value="sushi, nidogen and EGF-like domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000309">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000472">
        <property type="entry name" value="Uncharacterized protein, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000391">
        <property type="entry name" value="Weary, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3310">
        <property type="match status" value="1"/>
    </dbReference>
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    <sequence length="2482" mass="268605" checksum="981289799BB76423" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PRG8</accession>
    <name>A0A2D0PRG8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15 16">Zinc finger and BTB domain-containing protein 47</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15 16" type="primary">zbtb47b</name>
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    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
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    <reference evidence="15 16" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15 16">Blood</tissue>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
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            <begin position="207"/>
            <end position="235"/>
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    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
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    <feature type="domain" description="C2H2-type" evidence="13">
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    <feature type="domain" description="C2H2-type" evidence="13">
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
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            <begin position="13"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="12">
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    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
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    <feature type="compositionally biased region" description="Pro residues" evidence="12">
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    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017309161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_053530821.1"/>
        </source>
    </evidence>
    <sequence length="505" mass="58030" checksum="77038EBA275CA919" modified="2017-12-20" version="1">MWTLLGINEVVDNTKSRLEHHTSSQAQAKHFYKKENEGGGSGRGLCKSEGGTSPEEAVPQESPASFNREQIIVEVNLNNQTLNVSKGSEGKNRERRQTHHCTTSDEDKDYEQRQEEMEISTEEEKYDEDEEEDDDDDDDDDDDDDYEEENDRSIPEVPQAIMERPRRGTRSSAISLCQGLGGGMLAQGKKEQESLGQKVKLEEKQHFACKKCPRVFNNRWYLEKHTNVTHNRMQICNKCGKRFLLESELLLHHQTSCEKSIQCVTCGKAFKKLWSLHEHNKVVHGYAEKKYSCEVCEKKFYTMAHVRKHMVAHTKDMPFTCETCGKSFKRSMSLKVHSLQHSGEKPFRCENCSERFQYKYQLRSHMSIHIGHKQFMCQWCGKDFNMKQYFDEHMKTHTGEKPYICEICGKSFTSRPNMKRHRRTHTGEKPYPCDVCGQRFRFSNMLKAHKEKCFRLSSSLGDDSSMTSGGEGSHPSSLPLLHSISGLPPSPPPPPPPPFSNSSND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PSM5</accession>
    <name>A0A2D0PSM5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ferroxidase</fullName>
            <ecNumber evidence="4">1.16.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC108256549</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.16.3.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017309017.1">
        <property type="nucleotide sequence ID" value="XM_017453528.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PSM5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013867"/>
    <dbReference type="GeneID" id="108256549"/>
    <dbReference type="KEGG" id="ipu:108256549"/>
    <dbReference type="OMA" id="KATHAYS"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000009">
        <property type="entry name" value="Ceruloplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000028">
        <property type="entry name" value="Ceruloplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000075">
        <property type="entry name" value="hephaestin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000002">
        <property type="entry name" value="Hephaestin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024715">
        <property type="entry name" value="Factor_5/8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF504">
        <property type="entry name" value="PLASTOCYANIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000354">
        <property type="entry name" value="Factors_V_VIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 18" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0915">Sodium</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012587411" description="ferroxidase" evidence="18">
        <location>
            <begin position="21"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="20">
        <location>
            <begin position="87"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="19">
        <location>
            <begin position="267"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="20">
        <location>
            <begin position="443"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="20">
        <location>
            <begin position="796"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="19">
        <location>
            <begin position="929"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="166"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="271"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="520"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="627"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000354-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017309017.1"/>
        </source>
    </evidence>
    <sequence length="1076" mass="121976" checksum="8FBF4E59523F858D" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PTW0</accession>
    <name>A0A2D0PTW0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein naked cuticle homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC108256774</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Cell autonomous antagonist of the canonical Wnt signaling pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the NKD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017309469.1">
        <property type="nucleotide sequence ID" value="XM_017453980.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PTW0"/>
    <dbReference type="GeneID" id="108256774"/>
    <dbReference type="OrthoDB" id="5953812at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040140">
        <property type="entry name" value="Nkd-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22611">
        <property type="entry name" value="PROTEIN NAKED CUTICLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22611:SF1">
        <property type="entry name" value="PROTEIN NAKED CUTICLE HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="229"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="356"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="237"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017309469.1"/>
        </source>
    </evidence>
    <sequence length="377" mass="43028" checksum="3D43F239E778334E" modified="2017-12-20" version="1">MGLLQSKNACKRRESPEGGTLATSSLLVQGESAHPSESHKIRNKQDMCGTELMDDHFLDHDCPLEERTAECQSYKVSSESADPPRRNAKTDDMECNVPLVDKNNQEWVFTLYNFDNSGRVTKEDMSTLMHTIYEVVEASVKQTALCDSKTLRVKLTVTPLISSSNRRERDISLPQNVQDHLEEHHPIHIRGQSTNPAVSSEKKIYRIDENTERRNHYLDLAGIENYTSKFNADHPHQSPNEEQCQDSPTYGTSSAHHRWPQLDRGSLGKSGGRRLPFLRSLRSRSKSNRIYGHHPVSWCCPPVPQQQSLHYSHSKRVHARPRDAKSASVPPLGLDLELQPGGISPGGFMPMAQRHEHHHLHEHHHHHHHHHHHYYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PW12</accession>
    <name>A0A2D0PW12_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Free fatty acid receptor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC108256693</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017309315.1">
        <property type="nucleotide sequence ID" value="XM_017453826.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PW12"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034633"/>
    <dbReference type="GeneID" id="108256693"/>
    <dbReference type="KEGG" id="ipu:108256693"/>
    <dbReference type="OrthoDB" id="5961208at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071398">
        <property type="term" value="P:cellular response to fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15170">
        <property type="entry name" value="7tmA_FFAR2_FFAR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013312">
        <property type="entry name" value="GPR40-rel_orph"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45822">
        <property type="entry name" value="FREE FATTY ACID RECEPTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45822:SF8">
        <property type="entry name" value="FREE FATTY ACID RECEPTOR 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01904">
        <property type="entry name" value="GPR40FAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="80"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017309315.1"/>
        </source>
    </evidence>
    <sequence length="382" mass="44073" checksum="946D149519D2DF7F" modified="2017-12-20" version="1">MIKYHTFTQCHLARKRGCKCPFQCVSRFPNHYIEHSSVLSDTSKQEMSHELTKPNMVWTTDLSNLVLAVDAITLIAGVPANLLALYTFIQKVKQRCTPIDVLLLSLTISDLIFLFFLPFRMKEAADMEWTLSYFLCPLSGFFFYTTIYNSTFHLTAISVERYLGVAFPIKYKLKRRPRYAVIACVMFWVVSMAHCSIVYIMQYYRHNNESFTDPSKKEVCYDGFSDEQLNTLLPVRLELFLVLFCTPFIICSFCYIKFIHILCHLPNIKPKKRFRAIGLALATLLVFIICFMPYNVSHVVGYIKWESPAWRVYALLPSTLNACLDPLVFYFSSSALRGTLKHLIQGSNKRRHPSGCRNFLNCPKLNCTAHEESTQSSNDTSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PXG5</accession>
    <name>A0A2D0PXG5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation factor 1-alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC100526699</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Translation elongation factor that catalyzes the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis. Base pairing between the mRNA codon and the aa-tRNA anticodon promotes GTP hydrolysis, releasing the aa-tRNA from EEF1A1 and allowing its accommodation into the ribosome. The growing protein chain is subsequently transferred from the P-site peptidyl tRNA to the A-site aa-tRNA, extending it by one amino acid through ribosome-catalyzed peptide bond formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017309805.1">
        <property type="nucleotide sequence ID" value="XM_017454316.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PXG5"/>
    <dbReference type="GeneID" id="100526699"/>
    <dbReference type="KEGG" id="ipu:100526699"/>
    <dbReference type="OrthoDB" id="342024at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000003">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000090">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_A">
        <property type="entry name" value="EF_Tu_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008969">
        <property type="entry name" value="PRK12317.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10 13" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="7 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="5"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017309805.1"/>
        </source>
    </evidence>
    <sequence length="462" mass="50054" checksum="2E0742C10A120562" modified="2017-12-20" version="1">MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETSKYYITIIDAPGHRDFIKNMITGTSQADCAVLIVAGGVGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSQARFEEITKEVSAYIKKIGYNPAAVAFVPISGWHGDNMLEPSTNMGWFKGWKVERKEGNASGTTLLDALDAILPPSRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHESLPEATPGDNVGFNVKNVSVKDIRRGNVAGDSKNDPPQEAGSFTAQVIILNHPGQISQGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDNPKNLKSGDAAIVEMVPGKPMCVESFSTYPPLGRFAVRDMRQTVAVGVIKSVEKKAAGAGKVTKSAQKAAKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q288</accession>
    <name>A0A2D0Q288_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">All-trans retinoic acid-induced differentiation factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">atraid</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017311470.1">
        <property type="nucleotide sequence ID" value="XM_017455981.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q288"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000024545"/>
    <dbReference type="GeneID" id="108257874"/>
    <dbReference type="KEGG" id="ipu:108257874"/>
    <dbReference type="CTD" id="51374"/>
    <dbReference type="OMA" id="KMAPHGP"/>
    <dbReference type="OrthoDB" id="9989713at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045669">
        <property type="term" value="P:positive regulation of osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042350">
        <property type="entry name" value="ATRAID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15926">
        <property type="entry name" value="ALL-TRANS RETINOIC ACID-INDUCED DIFFERENTIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15926:SF1">
        <property type="entry name" value="ALL-TRANS RETINOIC ACID-INDUCED DIFFERENTIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="2">
        <location>
            <begin position="182"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017311470.1"/>
        </source>
    </evidence>
    <sequence length="230" mass="25407" checksum="BFE528EAAFE90AD1" modified="2017-12-20" version="1">MTAGVSIFAFSVVCLFSVLVILSRVYVDCQLTDAQLCHMCEGAIQNHSAVWRFCLSEGRIEGRCCLQDEEENILGLDLSNCSLSQVEDLHVASAAVIVDLSSNPISNMSDFIFQGFNYLSHLILPIKLDCPGGNTSWDRVDVNHDTRLCEGQRNACNQTGQMSLDCPENSICMPYGPGFVQCSCTHNFHGYKCLREGHFPMLEVMAVLGGSTVVVSMLLWITQRRKVKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q8Z4</accession>
    <name>A0A2D0Q8Z4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dipeptidase</fullName>
            <ecNumber evidence="3">3.4.13.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">dpep2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6 7">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>an L-aminoacyl-L-amino acid + H2O = 2 an L-alpha-amino acid</text>
            <dbReference type="Rhea" id="RHEA:48940"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
            <dbReference type="ChEBI" id="CHEBI:77460"/>
            <dbReference type="EC" id="3.4.13.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Membrane</location>
            <topology evidence="1 3">Lipid-anchor</topology>
            <topology evidence="1 3">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-dependent hydrolases superfamily. Peptidase M19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.13.19" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017314181.1">
        <property type="nucleotide sequence ID" value="XM_017458692.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017314182.1">
        <property type="nucleotide sequence ID" value="XM_017458693.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q8Z4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000025350"/>
    <dbReference type="GeneID" id="108259315"/>
    <dbReference type="KEGG" id="ipu:108259315"/>
    <dbReference type="CTD" id="64174"/>
    <dbReference type="OMA" id="SRHNVFG"/>
    <dbReference type="OrthoDB" id="445695at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070573">
        <property type="term" value="F:metallodipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01301">
        <property type="entry name" value="rDP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000030">
        <property type="entry name" value="Dipeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000180">
        <property type="entry name" value="Dipep_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008257">
        <property type="entry name" value="Pept_M19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10443:SF9">
        <property type="entry name" value="DIPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10443">
        <property type="entry name" value="MICROSOMAL DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01244">
        <property type="entry name" value="Peptidase_M19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00869">
        <property type="entry name" value="RENAL_DIPEPTIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51365">
        <property type="entry name" value="RENAL_DIPEPTIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0224">Dipeptidase</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2 3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013419717" description="Dipeptidase" evidence="3">
        <location>
            <begin position="28"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="405"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU341113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017314181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017314182.1"/>
        </source>
    </evidence>
    <sequence length="430" mass="47815" checksum="25423AC18F7E171A" modified="2017-12-20" version="1">MLTLKGLGVCELSLCCAFLLLLRGVQSDSSKTLELMSMYRLIDGHNDLPLHLRMLGNNKLSQFDLYNSPKTATDITRLKAGHVGGQMFAAYVLCTAQDKDAVRLALEQIDVIRRMCTEYPELELATTAAEMNATKKIACLISVEGGHAIDSSLPALRMFYQLGVRSMALTHTCNTPWAESSSLYYPYYKRENKSLTEFGKAVVHEMNRLGMLIDLSHSSWATAEAVLQLSKAPVIFSHSAAYKICNNPRNVPDKLLLMLKANGGLIMVNFYSDFIACGDQSDVSLVADHFDYLKHVIGAEHIGIGSDYDGASGFPQGLEDVSKYPALIGELLRRHWSEADLAGVLRLNFLRVFEKVEKIRDELRNTLPSEVSIPLAEAQNECRLVLRHPNSSKMVKSSGMQASNLLGSGALFWTVLFFHFMLLHKPTYLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QAR7</accession>
    <name>A0A2D0QAR7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">Zona pellucida sperm-binding protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC108259470</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Zona pellucida</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Cell membrane</location>
            <topology evidence="14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.</text>
    </comment>
    <comment type="PTM">
        <text evidence="14">Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the ZP domain family. ZPC subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017314475.1">
        <property type="nucleotide sequence ID" value="XM_017458986.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QAR7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020964"/>
    <dbReference type="GeneID" id="108259470"/>
    <dbReference type="KEGG" id="ipu:108259470"/>
    <dbReference type="OrthoDB" id="8880842at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035805">
        <property type="term" value="C:egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032190">
        <property type="term" value="F:acrosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035804">
        <property type="term" value="F:structural constituent of egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035803">
        <property type="term" value="P:egg coat formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000344">
        <property type="term" value="P:positive regulation of acrosome reaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.3210:FF:000001">
        <property type="entry name" value="Zona pellucida sperm-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.4100:FF:000002">
        <property type="entry name" value="Zona pellucida sperm-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055356">
        <property type="entry name" value="ZP-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048290">
        <property type="entry name" value="ZP_chr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576:SF2">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23344">
        <property type="entry name" value="ZP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00023">
        <property type="entry name" value="ZPELLUCIDA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 14" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="12 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 14" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 14" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025707581" description="Zona pellucida sperm-binding protein 3" evidence="14">
        <location>
            <begin position="22"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="16">
        <location>
            <begin position="124"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="32"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017314475.1"/>
        </source>
    </evidence>
    <sequence length="434" mass="46998" checksum="21D2925561D32835" modified="2017-12-20" version="1">MGFNQVGFAVLLLLALELSAAQWPGLNVPAGLQPNKPGHPSLQVSAGLPSQWMQSGPQSGSPPVAPLGLHLQNPSDAQDQQVMQRPVKKLIWRFPAAPQIPTPPAPVNFMRQSNAVPTQSVTARCNETAVYVEVRKDLFGTSAPLNIAALMLGGCAAKGVDVSSQVLVYESPLHGCNSKLTVTANELVYIFTLGIASVPLSSAPILRSPGAMAFVECHYPRFHNVSSSALLPAWIPYASAQAAEEHLVFSLRLMMDDWISERPSNYNYQLGELINIEASVVQFSHVPLRVFVDSCVATAVPDVNAVPRYSFIENHGCLLDAKLTQSSSHFMPQTQAAKLGFQLEAFKFQQVNSSWFYIACILKATAASAPADAEHKACSFSDNRWIAAYGVDQVCSCCSSSCGSRKGRDLPSEQDLQMEREVSLGPIMVLENVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QFI7</accession>
    <name>A0A2D0QFI7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">LIM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17 18" type="primary">lims2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17 18">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Within the IPP (ILK-PINCH-PARVIN) complex, binds to F-actin, promoting F-actin bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017317438.1">
        <property type="nucleotide sequence ID" value="XM_017461949.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053536811.1">
        <property type="nucleotide sequence ID" value="XM_053680836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QFI7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000028926"/>
    <dbReference type="GeneID" id="108261084"/>
    <dbReference type="KEGG" id="ipu:108261084"/>
    <dbReference type="CTD" id="55679"/>
    <dbReference type="GeneTree" id="ENSGT00940000153518"/>
    <dbReference type="OMA" id="ASWHAEC"/>
    <dbReference type="OrthoDB" id="20689at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045216">
        <property type="term" value="P:cell-cell junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001046">
        <property type="term" value="P:positive regulation of integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900026">
        <property type="term" value="P:positive regulation of substrate adhesion-dependent cell spreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09331">
        <property type="entry name" value="LIM1_PINCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09332">
        <property type="entry name" value="LIM2_PINCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09333">
        <property type="entry name" value="LIM3_PINCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09334">
        <property type="entry name" value="LIM4_PINCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09335">
        <property type="entry name" value="LIM5_PINCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000011">
        <property type="entry name" value="Lim and senescent cell antigen-like-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000017">
        <property type="entry name" value="Lim and senescent cell antigen-like-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000019">
        <property type="entry name" value="Lim and senescent cell antigen-like-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000021">
        <property type="entry name" value="Lim and senescent cell antigen-like-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000062">
        <property type="entry name" value="LIM domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047944">
        <property type="entry name" value="LIMS1/2-like_LIM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017351">
        <property type="entry name" value="PINCH-1-4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047946">
        <property type="entry name" value="PINCH-1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24210:SF12">
        <property type="entry name" value="LIM AND SENESCENT CELL ANTIGEN-LIKE-CONTAINING DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24210">
        <property type="entry name" value="LIM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038003">
        <property type="entry name" value="PINCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 13 14" id="KW-0440">LIM domain</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="69"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="132"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="252"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="312"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR038003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017317438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_053536811.1"/>
        </source>
    </evidence>
    <sequence length="394" mass="45399" checksum="B3B29CB0151066BA" modified="2017-12-20" version="1">MEAQKRGIPTSIPENEELIEVQMNGVRQNGGEAEVPLSKSQRRRSDVKVYKEFCDFYARFNMANALANAICELCKAGFTPAEKIVNSNGELYHEQCFVCAQCFQQFSEGLYYEFDDRKYCEHDFHMLFAPCCKQCGEFITGRVIKAMNNSWHPECFCCDICHTVLADVGFAKNAGRHLCRPCHLRERACGLGKYICQKCHTIIDEQPLIFRNDPYHPDHFNCSNCGKELTADARELKNELYCLPCHDKMGVPICRACRRPIEGRVVNALGKQWHVEHFVCAKCEKPFLGHQHYERKGLAYCETHFNLLFGDVCHHCNRVIEGEVISALNKAWCINCFACSTCSSKLTLKDKFVELDLKPICKQCYERMPTQLKYRLAKREHDSKDRRKKIGICL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QFQ3</accession>
    <name>A0A2D0QFQ3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation machinery-associated protein 16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tma16</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the biogenesis of the 60S ribosomal subunit in the nucleus.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Associates with pre-60S ribosomal particles.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TMA16 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017317134.3">
        <property type="nucleotide sequence ID" value="XM_017461645.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QFQ3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000004133"/>
    <dbReference type="GeneID" id="108260919"/>
    <dbReference type="KEGG" id="ipu:108260919"/>
    <dbReference type="CTD" id="55319"/>
    <dbReference type="OMA" id="SWFLGQI"/>
    <dbReference type="OrthoDB" id="270284at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.170:FF:000001">
        <property type="entry name" value="Translation machinery-associated 16 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.170">
        <property type="entry name" value="Translation machinery-associated protein 16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021346">
        <property type="entry name" value="Tma16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038356">
        <property type="entry name" value="Tma16_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13349">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13349:SF2">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11176">
        <property type="entry name" value="Tma16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="162"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="173"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017317134.3"/>
        </source>
    </evidence>
    <sequence length="201" mass="23519" checksum="5E31BE8E72BC586D" modified="2023-06-28" version="3">MPKALKGKSAVEKKVIHPYSRKAACLARSAHRQDRKERLKSEKAQRLNLIREKLSWFQSQLDSGKVEYSKQDACEIIERYLHRFDGELEQIELANSIKGRQGRLHFSRETVIKQTMERERAQYEGNGFEIPDIINSKHLKTFREWNGDLKKLPNIKMRKVSAKKNTAVKETHTEEEEHEEDEAEEEDVSQDSDVELLEDEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QHK8</accession>
    <name>A0A2D0QHK8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Trace amine-associated receptor 13c-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC108261629</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017317938.1">
        <property type="nucleotide sequence ID" value="XM_017462449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QHK8"/>
    <dbReference type="GeneID" id="108261629"/>
    <dbReference type="KEGG" id="ipu:108261629"/>
    <dbReference type="OMA" id="GECYLVL"/>
    <dbReference type="OrthoDB" id="10042731at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001594">
        <property type="term" value="F:trace-amine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009132">
        <property type="entry name" value="TAAR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF381">
        <property type="entry name" value="TRACE AMINE ASSOCIATED RECEPTOR 19P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01830">
        <property type="entry name" value="TRACEAMINER"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="28"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="143"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="44"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017317938.1"/>
        </source>
    </evidence>
    <sequence length="330" mass="37426" checksum="7F5BA4BF694C14BD" modified="2017-12-20" version="1">MTLTEFNQSDRCEHFSCPERSGSPAVYILLYVCSAAVVLLTVCGNMLVIISVLHFKQLHTPTNLLVFSLAVSDFLVGAFVMPSMLMWTIESCWIFGRDFCTSFWLISGFIAISSIYDIALIAVDRYLALSNPFLYMNRVSVRITGIVIVCVWCVVMAYSSALLYFNGNFTNTVMCPGECYLVLNEVSYVIDLIFSFIFPLSVIIILYTRVFVIAKKHATAIRELNNHTRPKTQKITSHSMKSERKAAKVLGILVSVFLMCLLPYFIYSLLGDVIEIQTETFLKLVILSCLNSTINPVIYALFYPWFRRCIKLIITLRIFQTDSALINMLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QK10</accession>
    <name>A0A2D0QK10_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Gamma-aminobutyric acid receptor subunit pi</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">GABA(A) receptor subunit pi</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC108262825</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">SDA103</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Pi subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA). GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interfaces. When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient. Pi-containing GABAARs are mostly located in peripheral tissues. In the uterus, pi subunits modulate uterus contraction by altering the sensitivity of GABAARs to pregnanolone. In the lungs, pi-containing GABAARs contribute to pulmonary fluid transport via luminal secretion of chloride.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="17">Heteropentamer, formed by a combination of alpha (GABRA1-6), beta (GABRB1-3), gamma (GABRG1-3), delta (GABRD), epsilon (GABRE), rho (GABRR1-3), pi (GABRP) and theta (GABRQ) chains, each subunit exhibiting distinct physiological and pharmacological properties.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRP sub-subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017318662.1">
        <property type="nucleotide sequence ID" value="XM_017463173.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QK10"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000028057"/>
    <dbReference type="GeneID" id="108262825"/>
    <dbReference type="KEGG" id="ipu:108262825"/>
    <dbReference type="OMA" id="PMSVMKR"/>
    <dbReference type="OrthoDB" id="8890589at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004890">
        <property type="term" value="F:GABA-A receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022851">
        <property type="term" value="F:GABA-gated chloride ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19004">
        <property type="entry name" value="LGIC_ECD_GABAAR_pi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000011">
        <property type="entry name" value="Gamma-aminobutyric acid receptor subunit pi isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0868">Chloride</keyword>
    <keyword evidence="10" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 20" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 20" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 20" id="KW-0732">Signal</keyword>
    <keyword evidence="4 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 20" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022252030" description="Gamma-aminobutyric acid receptor subunit pi" evidence="20">
        <location>
            <begin position="20"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="436"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="22">
        <location>
            <begin position="37"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="23">
        <location>
            <begin position="245"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="357"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00061437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00064898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00070417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00079775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_017318662.1"/>
        </source>
    </evidence>
    <sequence length="456" mass="52097" checksum="CCA977967015C707" modified="2017-12-20" version="1">MRPSCFILFAFILFSSILEEPLLSAEVKEGEILPPTIQKLMKGYNKYLRPFFDDGPVTVGMSLDIASIDKISEINMDYTATIFLRQRWTDERLVFEGNKSLSLDGRLVELLWVPDTFIVDSKKSFLHDITVENRLIRIFPNGTVLYALRITTTVACNMDLTKYPMDKQTCTLQLESWGYNVNDVMFYWVRGNDSVSGLDTLRLAQYTVEDHYTSASEAVYETGNYPKLIFHFELKRSILYFILETYVPSSLLVVLSWVSFWISQSSVPARICIGVTTVLTMTTLMMGARTSLPNANCFIKAIDVYLGICFSFIFGALIEYAVAHFCTLQHPEYNSAFTHMQDCEEEINGIVTTIASDSSRAKRRKDTPTPAPTSTPSGEPDLMAQTPSHLEPMTPEALEDPLNRCIRILSLIRQMLRSVNCCHVENPHYIDNYSRVTFPLSFVVVNLLYWTYYLYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QNC5</accession>
    <name>A0A2D0QNC5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vinculin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Metavinculin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">vcla</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">SDA103</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell junction</location>
            <location evidence="5">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <location evidence="4">Sarcolemma</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Podosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the vinculin/alpha-catenin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017319226.1">
        <property type="nucleotide sequence ID" value="XM_017463737.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QNC5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000031382"/>
    <dbReference type="GeneID" id="108263186"/>
    <dbReference type="KEGG" id="ipu:108263186"/>
    <dbReference type="CTD" id="552980"/>
    <dbReference type="OMA" id="INFETHA"/>
    <dbReference type="OrthoDB" id="29742at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030016">
        <property type="term" value="C:myofibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002102">
        <property type="term" value="C:podosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000041">
        <property type="entry name" value="Vinculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000010">
        <property type="entry name" value="Vinculin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.810:FF:000001">
        <property type="entry name" value="Vinculin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000013">
        <property type="entry name" value="Vinculin b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.810:FF:000002">
        <property type="entry name" value="Vinculin b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.810:FF:000003">
        <property type="entry name" value="Vinculin b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.230">
        <property type="entry name" value="Alpha-catenin/vinculin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.810">
        <property type="entry name" value="Vinculin, Vh2 four-helix bundle"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036723">
        <property type="entry name" value="Alpha-catenin/vinculin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017997">
        <property type="entry name" value="Vinculin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006077">
        <property type="entry name" value="Vinculin/catenin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000633">
        <property type="entry name" value="Vinculin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46180">
        <property type="entry name" value="VINCULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01044">
        <property type="entry name" value="Vinculin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00806">
        <property type="entry name" value="VINCULIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47220">
        <property type="entry name" value="alpha-catenin/vinculin-like"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00663">
        <property type="entry name" value="VINCULIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00664">
        <property type="entry name" value="VINCULIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="12" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="13" id="KW-0965">Cell junction</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="22" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="19" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="836"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="22">
        <location>
            <begin position="361"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="22">
        <location>
            <begin position="539"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="23">
        <location>
            <begin position="861"/>
            <end position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00014125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00024757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00033411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_017319226.1"/>
        </source>
    </evidence>
    <sequence length="1131" mass="124426" checksum="F6CA152C576E35C3" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QPE3</accession>
    <name>A0A2D0QPE3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PDZ domain-containing protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">pdzd7a</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">In cochlear developing hair cells, essential in organizing the USH2 complex at stereocilia ankle links. Blocks inhibition of adenylate cyclase activity mediated by ADGRV1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimerizes (via PDZ2 domain). Component of USH2 complex, composed of ADGRV1, PDZD7, USH2A and WHRN. Interacts (via PDZ domains) with WHRN; the interaction is direct. Interacts with USH1G. Interacts with ADGRV1 (via the cytoplasmic region). Interacts with USH2A (via the cytoplasmic region). Interacts with MYO7A (via MyTH4-FERM domains).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Stereocilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017320338.1">
        <property type="nucleotide sequence ID" value="XM_017464849.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QPE3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008310"/>
    <dbReference type="GeneID" id="108263748"/>
    <dbReference type="KEGG" id="ipu:108263748"/>
    <dbReference type="CTD" id="100332580"/>
    <dbReference type="OrthoDB" id="10029564at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002142">
        <property type="term" value="C:stereocilia ankle link complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032426">
        <property type="term" value="C:stereocilium tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060088">
        <property type="term" value="P:auditory receptor cell stereocilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07358">
        <property type="entry name" value="HN_PDZD7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10833">
        <property type="entry name" value="PDZ1_PDZD7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10834">
        <property type="entry name" value="PDZ2_PDZD7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06751">
        <property type="entry name" value="PDZ3_PDZD7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.20:FF:000007">
        <property type="entry name" value="PDZ domain containing 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000090">
        <property type="entry name" value="PDZ domain containing 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000092">
        <property type="entry name" value="PDZ domain containing 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000171">
        <property type="entry name" value="PDZ domain containing 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042786">
        <property type="entry name" value="PDZD7_HN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051844">
        <property type="entry name" value="USH2_Complex_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23116">
        <property type="entry name" value="PDZ DOMAIN CONTAINING WHIRLIN AND HARMONIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23116:SF29">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ" evidence="12">
        <location>
            <begin position="71"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="12">
        <location>
            <begin position="195"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="12">
        <location>
            <begin position="840"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="302"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="348"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="438"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="489"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="714"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="351"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="438"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="725"/>
            <end position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017320338.1"/>
        </source>
    </evidence>
    <sequence length="933" mass="102673" checksum="1FAB0E563D7C26A0" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QPJ8</accession>
    <name>A0A2D0QPJ8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Methylcytosine dioxygenase TET</fullName>
            <ecNumber evidence="11">1.14.11.80</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">tet2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming during embryonic development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>a 5-formyl-2'-deoxycytidine in DNA + 2-oxoglutarate + O2 = a 5-carboxyl-2'-deoxycytidine in DNA + succinate + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53832"/>
            <dbReference type="Rhea" id="RHEA-COMP:13656"/>
            <dbReference type="Rhea" id="RHEA-COMP:13657"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:137731"/>
            <dbReference type="ChEBI" id="CHEBI:137732"/>
            <dbReference type="EC" id="1.14.11.80"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>a 5-hydroxymethyl-2'-deoxycytidine in DNA + 2-oxoglutarate + O2 = a 5-formyl-2'-deoxycytidine in DNA + succinate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:53828"/>
            <dbReference type="Rhea" id="RHEA-COMP:13315"/>
            <dbReference type="Rhea" id="RHEA-COMP:13656"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:136731"/>
            <dbReference type="ChEBI" id="CHEBI:137731"/>
            <dbReference type="EC" id="1.14.11.80"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 5-methyl-2'-deoxycytidine in DNA + 2-oxoglutarate + O2 = a 5-hydroxymethyl-2'-deoxycytidine in DNA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:52636"/>
            <dbReference type="Rhea" id="RHEA-COMP:11370"/>
            <dbReference type="Rhea" id="RHEA-COMP:13315"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:85454"/>
            <dbReference type="ChEBI" id="CHEBI:136731"/>
            <dbReference type="EC" id="1.14.11.80"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="11">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">The zinc ions have a structural role.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TET family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.80" evidence="11"/>
    <dbReference type="RefSeq" id="XP_017320393.1">
        <property type="nucleotide sequence ID" value="XM_017464904.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000002768"/>
    <dbReference type="GeneID" id="108263772"/>
    <dbReference type="KEGG" id="ipu:108263772"/>
    <dbReference type="CTD" id="54790"/>
    <dbReference type="OMA" id="QQPNNHC"/>
    <dbReference type="OrthoDB" id="8854879at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070579">
        <property type="term" value="F:DNA 5-methylcytosine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141166">
        <property type="term" value="P:chromosomal 5-methylcytosine DNA demethylation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030099">
        <property type="term" value="P:myeloid cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024779">
        <property type="entry name" value="2OGFeDO_JBP1/TET_oxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040175">
        <property type="entry name" value="TET1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046942">
        <property type="entry name" value="TET_oxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23358">
        <property type="entry name" value="METHYLCYTOSINE DIOXYGENASE TET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23358:SF3">
        <property type="entry name" value="METHYLCYTOSINE DIOXYGENASE TET2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12851">
        <property type="entry name" value="Tet_JBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01333">
        <property type="entry name" value="Tet_JBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 11 15" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8 11" id="KW-0408">Iron</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Methylcytosine dioxygenase TET1-3 oxygenase" evidence="13">
        <location>
            <begin position="1085"/>
            <end position="1675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="436"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="573"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="607"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="876"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1253"/>
            <end position="1310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1332"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1695"/>
            <end position="1734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="19"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="440"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="580"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="607"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1275"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1332"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1373"/>
            <end position="1382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1695"/>
            <end position="1707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017320393.1"/>
        </source>
    </evidence>
    <sequence length="1772" mass="195553" checksum="7434DAB8E29E2683" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QVD9</accession>
    <name>A0A2D0QVD9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Mediator of RNA polymerase II transcription subunit 18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">Mediator complex subunit 18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">med18</name>
        <name evidence="10" type="synonym">MED18</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12 13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 10">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Mediator complex subunit 18 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017322319.1">
        <property type="nucleotide sequence ID" value="XM_017466830.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017322387.1">
        <property type="nucleotide sequence ID" value="XM_017466898.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QVD9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021327"/>
    <dbReference type="GeneID" id="100528830"/>
    <dbReference type="CTD" id="54797"/>
    <dbReference type="OMA" id="ARGYMFR"/>
    <dbReference type="OrthoDB" id="10018982at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.320.10:FF:000001">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.320.10">
        <property type="entry name" value="Hypothetical Protein Pfu-838710-001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019095">
        <property type="entry name" value="Mediator_Med18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13321:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13321">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION, SUBUNIT 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09637">
        <property type="entry name" value="Med18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0010">Activator</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 10" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017322319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017322387.1"/>
        </source>
    </evidence>
    <sequence length="208" mass="23200" checksum="29B40571978FEE75" modified="2017-12-20" version="1">MEAPPVTVMPVTGGTINMMEYLLQGSVLDQSLDSLLHRLRGLCDNIEPESFADHELVYLLKGQQGNPFILRARRSLAHPTAPWHLRYLGQPEVGDKSRHALVRNCVDVAASHSLPDFLNEMGFRMDHEFVAKGHIFRKGALKVVVSKLSRVLVPGNTDNTEPLSLSYLVELSVLAPAGQDTVSEDMRSFAEQLKPLVHLEKIDPKRLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QYU0</accession>
    <name>A0A2D0QYU0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Zinc finger protein 646 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">zgc:66448</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017322921.1">
        <property type="nucleotide sequence ID" value="XM_017467432.3"/>
    </dbReference>
    <dbReference type="GeneID" id="108265278"/>
    <dbReference type="KEGG" id="ipu:108265278"/>
    <dbReference type="OMA" id="WLSELHP"/>
    <dbReference type="OrthoDB" id="8117402at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
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    <sequence length="1360" mass="151796" checksum="6222E12D673322A4" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QZA5</accession>
    <name>A0A2D0QZA5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Uridine-cytidine kinase</fullName>
            <ecNumber evidence="11">2.7.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">uck2a</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. Does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Can use ATP or GTP as a phosphate donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 11">
            <text>cytidine + ATP = CMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>uridine + ATP = UMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16825"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the uridine kinase family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017468233.3"/>
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    <dbReference type="KEGG" id="ipu:108265664"/>
    <dbReference type="CTD" id="100005763"/>
    <dbReference type="OMA" id="HRQPFLI"/>
    <dbReference type="OrthoDB" id="10257085at2759"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00637"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00579">
        <property type="reaction ID" value="UER00640"/>
    </dbReference>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043771">
        <property type="term" value="F:cytidine kinase activity"/>
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        <property type="project" value="RHEA"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0044211">
        <property type="term" value="P:CTP salvage"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
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        <property type="term" value="P:UMP salvage"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02023">
        <property type="entry name" value="UMPK"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Uridine-cytidine kinase 2"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="PRK/URK"/>
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        <property type="entry name" value="URIDINE KINASE"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00988">
        <property type="entry name" value="URIDINKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="13">
        <location>
            <begin position="25"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017323722.1"/>
        </source>
    </evidence>
    <sequence length="263" mass="29879" checksum="F769AFCF6D886EE0" modified="2017-12-20" version="1">MAGESEADLEQREPEHTHTIRQPFLIGVAGGTASGKSSVCGKIMELLGQNKIDHHQRQVAILSQDSFYKVLTPEQKAKALKGQFNFDHPDAFDNELIIKTLWDIMEGRTVQIPVYDFVTHSRKKETVTVYPADVVLFEGILMFYSQEIRDLFQMKLFVDTDADTRLSRRVLRDISERGRDLEQVLSQYITFVKPAFEEFCLPTKKYADVIIPRGADNLVAINLIVQHIQDILNGGLTKRLNGCLNGHTTPRKRQPSESSSRPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QZE3</accession>
    <name>A0A2D0QZE3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Axonemal dynein light intermediate polypeptide 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Inner dynein arm light chain, axonemal</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC108265256</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in sperm flagellum assembly.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the inner dynein arm light chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017322885.1">
        <property type="nucleotide sequence ID" value="XM_017467396.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QZE3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012520"/>
    <dbReference type="GeneID" id="108265256"/>
    <dbReference type="KEGG" id="ipu:108265256"/>
    <dbReference type="OMA" id="ARMTGIC"/>
    <dbReference type="OrthoDB" id="273640at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097546">
        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019347">
        <property type="entry name" value="Axonemal_dynein_light_chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13183:SF0">
        <property type="entry name" value="AXONEMAL DYNEIN LIGHT INTERMEDIATE POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13183">
        <property type="entry name" value="AXONEMAL INNER ARM DYNEIN LIGHT CHAIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10211">
        <property type="entry name" value="Ax_dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0243">Dynein</keyword>
    <keyword evidence="3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="172"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017322885.1"/>
        </source>
    </evidence>
    <sequence length="243" mass="28285" checksum="9A1C877479A68184" modified="2017-12-20" version="1">MFTESLLMYDTPVLVSKKTDESPKLQRKISGPDPCPPKPKSSSSDIIKQQTEELLNAMLPPREWRENDQLWVQKVSSTPCTRTDIIHLQEELDLKLQQRKANDTGVCPVRRELYTQCFDELVRQVTINCAERGLLLLRVRDEIRMTISSYQNLYESSVAFGMRKALEAEKEKSDNVKRIADLADELTELERQLNEKKAECEEMERQANEQRLVEEQKHAEEIESLKYTNLQIKDQLNTVITQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R1C6</accession>
    <name>A0A2D0R1C6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">RING finger protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">rnf11b</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of TNFAIP3 to RIPK1 after TNF stimulation. TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Recruits STAMBP to the E3 ubiquitin-ligase SMURF2 for ubiquitination, leading to its degradation by the 26S proteasome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Interacts (when phosphorylated) with 14-3-3. Interacts with the E3 ubiquitin-ligases NEDD4, ITCH, SMURF2 and WWP1. Also interacts with the E2 ubiquitin-conjugating enzymes UBE2D1 and UBE2N, but neither with CDC34, nor with UBE2L3. Interacts with ZNF350, EPS15 and STAMBP. After TNF stimulation, interacts with TAX1BP1, TNFAIP3 and RIPK1; these interactions are transient and they are lost after 1 hour of stimulation with TNF. Interacts with GGA1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017324453.1">
        <property type="nucleotide sequence ID" value="XM_017468964.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R1C6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000002053"/>
    <dbReference type="GeneID" id="108266025"/>
    <dbReference type="KEGG" id="ipu:108266025"/>
    <dbReference type="CTD" id="100150568"/>
    <dbReference type="OMA" id="HLDCIDN"/>
    <dbReference type="OrthoDB" id="9984778at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16468">
        <property type="entry name" value="RING-H2_RNF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000134">
        <property type="entry name" value="Ring finger protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042981">
        <property type="entry name" value="RNF11_RING-H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052804">
        <property type="entry name" value="UEC_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46359">
        <property type="entry name" value="GEO07743P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46359:SF1">
        <property type="entry name" value="RING FINGER PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0519">Myristate</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-0564">Palmitate</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="22">
        <location>
            <begin position="99"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00055330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00066037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00072629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_017324453.1"/>
        </source>
    </evidence>
    <sequence length="154" mass="17417" checksum="2239B087446E0687" modified="2017-12-20" version="1">MGNCLKSPTSDDISLLHESQSDRASFGDGTFPDQEPPPPYQEQIHVPVYHPTPSQTRLATQLTEEEQVRIAQRIGLIQHLPKGVFDPGSDGTEKKIRECVICMLDFVYGDPIRFLPCMHIYHMDCIDDWLMRSFTCPSCMEPVDAALLSSYETN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R1L2</accession>
    <name>A0A2D0R1L2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Interferon-induced GTP-binding protein Mx isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC108266073</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017324538.1">
        <property type="nucleotide sequence ID" value="XM_017469049.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R1L2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018334"/>
    <dbReference type="GeneID" id="108266073"/>
    <dbReference type="KEGG" id="ipu:108266073"/>
    <dbReference type="OMA" id="MKTWEAI"/>
    <dbReference type="OrthoDB" id="5061070at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016185">
        <property type="term" value="P:synaptic vesicle budding from presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1240:FF:000007">
        <property type="entry name" value="Interferon-induced GTP-binding protein Mx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000621">
        <property type="entry name" value="Interferon-induced GTP-binding protein Mx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003130">
        <property type="entry name" value="GED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF199">
        <property type="entry name" value="INTERFERON-INDUCED GTP-BINDING PROTEIN MXC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02212">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00302">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00410">
        <property type="entry name" value="G_DYNAMIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017324538.1"/>
        </source>
    </evidence>
    <sequence length="639" mass="72493" checksum="07C20F1D177F436B" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R1U9</accession>
    <name>A0A2D0R1U9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">wnt6b</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Secreted</location>
            <location evidence="1 10">Extracellular space</location>
            <location evidence="1 10">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017324607.1">
        <property type="nucleotide sequence ID" value="XM_017469118.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R1U9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000015210"/>
    <dbReference type="GeneID" id="108266110"/>
    <dbReference type="KEGG" id="ipu:108266110"/>
    <dbReference type="CTD" id="561831"/>
    <dbReference type="OMA" id="EWTNCNC"/>
    <dbReference type="OrthoDB" id="5945655at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19338">
        <property type="entry name" value="Wnt_Wnt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000001">
        <property type="entry name" value="Wnt homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009143">
        <property type="entry name" value="Wnt6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF72">
        <property type="entry name" value="PROTEIN WNT-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01845">
        <property type="entry name" value="WNT6PROTEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 10" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017324607.1"/>
        </source>
    </evidence>
    <sequence length="353" mass="39664" checksum="CB1FDAE1C1E28A8E" modified="2017-12-20" version="1">MHSQYQLALFFILLCPVNIIGMWWAVGSPLVMDPNSICRKTKRLGSKQADLCQNHPEIVTEVVKGARLGVRECQYQFRFRRWNCTSHNKYFGKILQQDIRETAFVYAITAAGVTHAVTQACSMGELLQCGCEAPPPRRADTPPIVDGAWDGGGAWEWGGCGDDVEFGYEKSKQFMDAKRRKDKSDIRTLIDLHNNEAGRLAVKNYMRTECKCHGLSGSCTLRTCWKKLPRFREVGNRLLERFNGASKVMGGNDGKTLIPVGQNIKAPDKQDLIYSAESPDFCLPNRKTGSPGTRGRTCNSTALDVSGCDLLCCERGYRDEEVVLEENCLCRFHWCCVVQCKKCSVRKELSLCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R2R7</accession>
    <name>A0A2D0R2R7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cohesin subunit SA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">SCC3 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Stromal antigen</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">si:ch211-269e2.1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6 7">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the cohesin complex which is composed of a heterodimer between a SMC1 protein (SMC1A or SMC1B) and SMC3, which are attached via their hinge domain, and RAD21 which link them at their heads, and one STAG protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the SCC3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017324897.1">
        <property type="nucleotide sequence ID" value="XM_017469408.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017324898.1">
        <property type="nucleotide sequence ID" value="XM_017469409.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R2R7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034654"/>
    <dbReference type="GeneID" id="108266307"/>
    <dbReference type="KEGG" id="ipu:108266307"/>
    <dbReference type="OMA" id="QCMSFAM"/>
    <dbReference type="OrthoDB" id="498590at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039662">
        <property type="entry name" value="Cohesin_Scc3/SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056396">
        <property type="entry name" value="HEAT_SCC3-SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020839">
        <property type="entry name" value="SCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013721">
        <property type="entry name" value="STAG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199:SF2">
        <property type="entry name" value="COHESIN SUBUNIT SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199">
        <property type="entry name" value="STROMAL ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24571">
        <property type="entry name" value="HEAT_SCC3-SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21581">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08514">
        <property type="entry name" value="STAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51425">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="2" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCD" evidence="4">
        <location>
            <begin position="318"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="14"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="14"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017324897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017324898.1"/>
        </source>
    </evidence>
    <sequence length="1176" mass="133526" checksum="9D68D7ED5EAA32E8" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R3J3</accession>
    <name>A0A2D0R3J3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Leucine-rich repeat flightless-interacting protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">lrrfip1a</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LRRFIP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017325167.2">
        <property type="nucleotide sequence ID" value="XM_017469678.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R3J3"/>
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11">SDA103</strain>
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        <citation type="submission" date="2025-04" db="UniProtKB">
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <tissue evidence="12 13 14">Blood</tissue>
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        <text evidence="8">Necessary for the splicing of pre-mRNA.</text>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017326098.1"/>
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    </evidence>
    <sequence length="464" mass="52203" checksum="43CFCEBF596D167C" modified="2017-12-20" version="1">MSDFDEFERQLTENKQAADRDKENKQHRRSSSRSRSRERDRKRRSRDRERRGSRDHHGDTKERRHRRSRSPHREKKKAKVKKYWDVPPPGFEHVTPVQYKAMQAAGQIPATALLPTMTSEGLAVTPSPVPVVGSQMTRQARRLYVGNIPFGMTEESMMDFFNAQMRLGGLTQALGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATCLSKGYAFCEYVDVNISDQAIAGLNGMQLGDKKLLVQRASVGSKNTTLPGINQTPVTLQVPGLMNSSMNQMGGIPTEVLCLMNMVSPEELLDDEEYEEIVEDVRDECSKYGQVKSIEIPRPVDGLEVPGTGKIFVEFMSVYDSQKAMQGLTGRKFANRVVVTKYCDPDAYHSRDFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R8V2</accession>
    <name>A0A2D0R8V2_ICTPU</name>
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        <recommendedName>
            <fullName evidence="18">Branchpoint-bridging protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">sf1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.</text>
    </comment>
    <comment type="function">
        <text evidence="18">Necessary for the splicing of pre-mRNA. Has a role in the recognition of the branch site (5'-UACUAAC-3'), the pyrimidine tract and the 3'-splice site at the 3'-end of introns.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the BBP/SF1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017327018.1">
        <property type="nucleotide sequence ID" value="XM_017471529.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R8V2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034116"/>
    <dbReference type="GeneID" id="108267458"/>
    <dbReference type="KEGG" id="ipu:108267458"/>
    <dbReference type="CTD" id="7536"/>
    <dbReference type="OMA" id="PGMPSMY"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045131">
        <property type="term" value="F:pre-mRNA branch point binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048024">
        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22382">
        <property type="entry name" value="KH-I_SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000016">
        <property type="entry name" value="Putative splicing factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032570">
        <property type="entry name" value="SF1-HH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047086">
        <property type="entry name" value="SF1-HH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF45">
        <property type="entry name" value="SPLICING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16275">
        <property type="entry name" value="SF1-HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 18" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="14 18" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="15 18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 18" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9 18" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 17 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="20">
        <location>
            <begin position="346"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="395"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="19">
        <location>
            <begin position="443"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="455"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="489"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="500"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="541"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="581"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="623"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="643"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00055181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU367126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017327018.1"/>
        </source>
    </evidence>
    <sequence length="660" mass="70386" checksum="E5DFB4D5771238B3" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R981</accession>
    <name>A0A2D0R981_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fibroblast growth factor</fullName>
            <shortName evidence="4">FGF</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">fgf11a</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017326635.1">
        <property type="nucleotide sequence ID" value="XM_017471146.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R981"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034292"/>
    <dbReference type="GeneID" id="108267160"/>
    <dbReference type="KEGG" id="ipu:108267160"/>
    <dbReference type="CTD" id="497640"/>
    <dbReference type="OMA" id="THLYCRH"/>
    <dbReference type="OrthoDB" id="6158176at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23309">
        <property type="entry name" value="beta-trefoil_FGF11-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000001">
        <property type="entry name" value="Fibroblast growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00263">
        <property type="entry name" value="HBGFFGF"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00442">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00247">
        <property type="entry name" value="HBGF_FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="212"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU049442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017326635.1"/>
        </source>
    </evidence>
    <sequence length="247" mass="28080" checksum="7BE534F0F3255ADE" modified="2017-12-20" version="1">MAALASSLIRQRREVKDQQANRQISNKRKPCPKSNKSLCQKQILILISKVRLCGSRGRKLEKRPEPQLKGIVTRLYCRHGFYLQMLPDGTMDGTKDENSSFLLFNLIPVGLRIVAIQSTKTGLYVAMNSEGYLYTSEHFTPECKFKECVFENYYVTYSSILYRQTQSGRAWYIGINRDGQVMKGNRVKKTKGAAHFLPKLIEVAMYREPSLHDIGEQSPPWKTAKTSDSPVNNDDKNDPLPPKTSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R9I1</accession>
    <name>A0A2D0R9I1_ICTPU</name>
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        <submittedName>
            <fullName evidence="7">DENN domain-containing protein 4B isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC108267550</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
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    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017327233.1">
        <property type="nucleotide sequence ID" value="XM_017471744.3"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="75250at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
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        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:regulation of Rab protein signal transduction"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="C-myc promoter-binding protein isoform X1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="dDENN_dom"/>
    </dbReference>
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        <property type="entry name" value="DENN_C"/>
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        <property type="entry name" value="DENN_Domain_GEFs"/>
    </dbReference>
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        <property type="entry name" value="MABP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
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        <property type="entry name" value="uDENN_dom"/>
    </dbReference>
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        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296:SF18">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51498">
        <property type="entry name" value="MABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MABP" evidence="5">
        <location>
            <begin position="37"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="UDENN" evidence="4">
        <location>
            <begin position="188"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="798"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="908"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="948"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
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        </location>
    </feature>
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        </location>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="1238"/>
            <end position="1250"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017327233.1"/>
        </source>
    </evidence>
    <sequence length="1487" mass="167270" checksum="C909AB368F64BC03" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0REM6</accession>
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        <submittedName>
            <fullName evidence="2">Synaptonemal complex central element protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">syce3</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SDA103</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017328994.1">
        <property type="nucleotide sequence ID" value="XM_017473505.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0REM6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027260"/>
    <dbReference type="GeneID" id="108268486"/>
    <dbReference type="KEGG" id="ipu:108268486"/>
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    <dbReference type="OrthoDB" id="9944849at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007130">
        <property type="term" value="P:synaptonemal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028145">
        <property type="entry name" value="Synaptonemal_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36686">
        <property type="entry name" value="SYNAPTONEMAL COMPLEX CENTRAL ELEMENT PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36686:SF1">
        <property type="entry name" value="SYNAPTONEMAL COMPLEX CENTRAL ELEMENT PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15191">
        <property type="entry name" value="Synaptonemal_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_017328994.1"/>
        </source>
    </evidence>
    <sequence length="94" mass="10751" checksum="A42DCE816EDF5BA1" modified="2017-12-20" version="1">MVDNASAGEQYEDFGRETLECSKDLERMTEQMEKISVNVTWMTYDMVVLRTDPQLAKSLKRLKNEFVQCKAVICGSGDNLVDQRIVANQKTTQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RJ38</accession>
    <name>A0A2D0RJ38_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">G/T mismatch-specific thymine DNA glycosylase</fullName>
            <ecNumber evidence="15">3.2.2.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Thymine-DNA glycosylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">tdg.1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Hydrolyzes mismatched double-stranded DNA and polynucleotides, releasing free thymine.</text>
            <dbReference type="EC" id="3.2.2.29"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homodimer. Interacts with AICDA and GADD45A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the uracil-DNA glycosylase (UDG) superfamily. TDG/mug family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.29" evidence="15"/>
    <dbReference type="RefSeq" id="XP_017330544.1">
        <property type="nucleotide sequence ID" value="XM_017475055.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RJ38"/>
    <dbReference type="GeneID" id="108269283"/>
    <dbReference type="CTD" id="553789"/>
    <dbReference type="OrthoDB" id="565731at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141016">
        <property type="term" value="F:G/T mismatch-specific thymine-DNA glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032183">
        <property type="term" value="F:SUMO binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10028">
        <property type="entry name" value="UDG-F2_TDG_MUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.470.10:FF:000002">
        <property type="entry name" value="G/T mismatch-specific thymine DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015637">
        <property type="entry name" value="MUG/TDG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12159">
        <property type="entry name" value="G/T AND G/U MISMATCH-SPECIFIC DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12159:SF11">
        <property type="entry name" value="G_T MISMATCH-SPECIFIC THYMINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="19">
        <location>
            <begin position="113"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="348"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="383"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00064519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00066769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00071248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00083221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF03167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017330544.1"/>
        </source>
    </evidence>
    <sequence length="393" mass="43574" checksum="DBD7E668216BBB0F" modified="2017-12-20" version="1">MANRNVAHFMEGHGEEPGMMEMPAQEPPAAPPEPGAPQKAPTKGKRGRPASKEPKAKPGPKPKKAKAVKAGQQAEGTQEKIDESFKKVKRKIDRFKGMSEEEVLKKTLPDILTYNLDYVIIGINPGLMAAYIGRWFPGPGNHFWKCLFLSGFTEQLLNHMHDETLPEKYGIGFTNMVARATPGSKDLSSKELREGGKILVEKIKKFKPLIAVFNGKCIYEMFCREIFGKKPKTLEFGLQPHRIPDSETALYLMPSSSARCAQFPRAQDKVHFYIKLRELRDELKGVAKPREVEEVNYTFDLGLAKEDAKRMAIKEEQYDPGYEAAYGGAYGEPGNEEGNGHCAFASGEAEAAEKQPRSPNAVSQLPDGQWMTNSFADQIPEIGSSSQVQAEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RK65</accession>
    <name>A0A2D0RK65_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">E3 ubiquitin-protein ligase Hakai</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Casitas B-lineage lymphoma-transforming sequence-like protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">RING-type E3 ubiquitin transferase Hakai</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21 22" type="primary">LOC108269280</name>
    </gene>
    <organism evidence="20 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21 22" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21 22">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the Hakai family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017330535.1">
        <property type="nucleotide sequence ID" value="XM_017475046.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053538221.1">
        <property type="nucleotide sequence ID" value="XM_053682246.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RK65"/>
    <dbReference type="GeneID" id="108269280"/>
    <dbReference type="KEGG" id="ipu:108269280"/>
    <dbReference type="OrthoDB" id="547746at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036396">
        <property type="term" value="C:RNA N6-methyladenosine methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030155">
        <property type="term" value="P:regulation of cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16508">
        <property type="entry name" value="RING-HC_HAKAI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000140">
        <property type="entry name" value="E3 ubiquitin-protein ligase Hakai isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.140.2210:FF:000001">
        <property type="entry name" value="Putative e3 ubiquitin-protein ligase hakai"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040380">
        <property type="entry name" value="HAKAI-like_RING-HC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040383">
        <property type="entry name" value="HAKAI/CBLL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041042">
        <property type="entry name" value="Znf_Hakai"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13480:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HAKAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13480">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HAKAI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18408">
        <property type="entry name" value="zf_Hakai"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="19">
        <location>
            <begin position="170"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="263"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="349"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="270"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="367"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="395"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="435"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="481"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038499"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00041081"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00076432"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00079012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017330535.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_053538221.1"/>
        </source>
    </evidence>
    <sequence length="542" mass="59505" checksum="B8BFC9993DBEAB96" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RNV0</accession>
    <name>A0A2D0RNV0_ICTPU</name>
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        <submittedName>
            <fullName evidence="14">Polycomb group protein ASXL2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">asxl2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Asx family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017332183.1">
        <property type="nucleotide sequence ID" value="XM_017476694.3"/>
    </dbReference>
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    <dbReference type="STRING" id="7998.ENSIPUP00000035181"/>
    <dbReference type="GeneID" id="108270232"/>
    <dbReference type="KEGG" id="ipu:108270232"/>
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    <dbReference type="OMA" id="WELKQSE"/>
    <dbReference type="OrthoDB" id="9348951at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
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    <dbReference type="GO" id="GO:0035517">
        <property type="term" value="C:PR-DUB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0042975">
        <property type="term" value="F:peroxisome proliferator activated receptor binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="ASX-like_PHD"/>
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        <property type="entry name" value="ASX/ASX-like"/>
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        <property type="entry name" value="ASX_DEUBAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007759">
        <property type="entry name" value="Asxl_HARE-HTH"/>
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    <dbReference type="InterPro" id="IPR044867">
        <property type="entry name" value="DEUBAD_dom"/>
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        <property type="entry name" value="ADDITIONAL SEX COMBS LIKE PROTEIN ASXL"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR13578:SF11">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN ASXL2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13919">
        <property type="entry name" value="ASXH"/>
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    </dbReference>
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        <property type="entry name" value="HARE-HTH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PHD_3"/>
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    </dbReference>
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        <property type="entry name" value="DEUBAD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HTH_HARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HTH HARE-type" evidence="11">
        <location>
            <begin position="11"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="DEUBAD" evidence="12">
        <location>
            <begin position="272"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="95"/>
            <end position="192"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="206"/>
            <end position="257"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="992"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="103"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="136"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="409"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="424"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="444"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="462"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="501"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="541"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="565"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="608"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="642"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="679"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="705"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017332183.1"/>
        </source>
    </evidence>
    <sequence length="1183" mass="128014" checksum="479366D063B57922" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RP93</accession>
    <name>A0A2D0RP93_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Transforming growth factor beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">tgfb3</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Required to maintain the Transforming growth factor beta-1 (TGF-beta-1) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-1 and regulates its activation via interaction with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS, that control activation of TGF-beta-1. Interaction with integrins (ITGAV:ITGB6 or ITGAV:ITGB8) results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-1.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Required to maintain the Transforming growth factor beta-3 (TGF-beta-3) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-3 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-3. Interaction with integrins results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-3.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Transforming growth factor beta-3 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains, which constitute the regulatory and active subunit of TGF-beta-3, respectively.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Transforming growth factor beta-3: Multifunctional protein that regulates embryogenesis and cell differentiation and is required in various processes such as secondary palate development. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains remain non-covalently linked rendering TGF-beta-3 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP that control activation of TGF-beta-3 and maintain it in a latent state during storage in extracellular milieus. TGF-beta-3 is released from LAP by integrins: integrin-binding results in distortion of the LAP chain and subsequent release of the active TGF-beta-3. Once activated following release of LAP, TGF-beta-3 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Latency-associated peptide: Homodimer; disulfide-linked. Latency-associated peptide: Interacts with Transforming growth factor beta-1 (TGF-beta-1) chain; interaction is non-covalent and maintains (TGF-beta-1) in a latent state; each Latency-associated peptide (LAP) monomer interacts with TGF-beta-1 in the other monomer. Transforming growth factor beta-1: Homodimer; disulfide-linked. Transforming growth factor beta-1: Interacts with TGF-beta receptors (tgfbr1 and tgfbr2), leading to signal transduction. Interacts with EFEMP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16 18">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017331981.1">
        <property type="nucleotide sequence ID" value="XM_017476492.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RP93"/>
    <dbReference type="SMR" id="A0A2D0RP93"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000011293"/>
    <dbReference type="GeneID" id="108270130"/>
    <dbReference type="KEGG" id="ipu:108270130"/>
    <dbReference type="CTD" id="7043"/>
    <dbReference type="OMA" id="SHMKMYV"/>
    <dbReference type="OrthoDB" id="6092228at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005160">
        <property type="term" value="F:transforming growth factor beta receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060218">
        <property type="term" value="P:hematopoietic stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061035">
        <property type="term" value="P:regulation of cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030278">
        <property type="term" value="P:regulation of ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19386">
        <property type="entry name" value="TGF_beta_TGFB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000004">
        <property type="entry name" value="Transforming growth factor beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.970:FF:000006">
        <property type="entry name" value="Transforming growth factor beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016319">
        <property type="entry name" value="TGF-beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015618">
        <property type="entry name" value="TGFB3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF34">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR BETA-3 PROPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001787">
        <property type="entry name" value="TGF-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01423">
        <property type="entry name" value="TGFBETA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01426">
        <property type="entry name" value="TGFBETA3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="9 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 16 18" id="KW-0339">Growth factor</keyword>
    <keyword evidence="11 16" id="KW-0497">Mitogen</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 16" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5016195798" description="Transforming growth factor beta" evidence="16">
        <location>
            <begin position="19"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="19">
        <location>
            <begin position="294"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="304"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="312"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="341"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="345"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Interchain" evidence="17">
        <location>
            <position position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00057824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00065283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR001787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR001787-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017331981.1"/>
        </source>
    </evidence>
    <sequence length="409" mass="47066" checksum="DA3DB245FD1D2755" modified="2017-12-20" version="1">MHLGKALLFFLLLNSVTTIFSLSTCTTVDIDHIKKKRVEAIRGQILSKLRLTSPPQALGPSQVPYPVLALYNSTKELIEELGRERQQSCGQDNTETEYYAKEIYKFNMIQGPPDNNDVVYCPKGKTSKVFRFNVSSMEKNSTNLFRAEFRALRVPNSSASRNEQRIELYQIIKHYEHIAKQRYIGGKNVLTKGTAEWVSFDVTETVREWLTHRETNLGLEVSVHCPCHTFNPNGDINDHKNEVLEVKFIGMDGDYYEHRVDHSRLSKQKEQPLPHLILMMLPPHRLNVPPTSRRRKRALDTNYCFSNYEENCCVRPLYIDFRQDLGWRWIHEPKGYHANFCSGPCPYLRSADTTHSSLLSLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMIVKSCKCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RUX0</accession>
    <name>A0A2D0RUX0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="13">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">polrmt</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="function">
        <text evidence="10">DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA. In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Has DNA primase activity. Catalyzes the synthesis of short RNA primers that are necessary for the initiation of lagging-strand DNA synthesis from the origin of light-strand DNA replication (OriL).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 13">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT. In this complex TFAM recruits POLRMT to the promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Upon metabolic stress, forms a complex composed of FOXO3, SIRT3 and mitochondrial RNA polymerase POLRMT; the complex is recruited to mtDNA in a SIRT3-dependent manner. Also forms a complex composed of FOXO3, SIRT3, TFAM and POLRMT. Interacts with TFB1M and TFB2M, leading to the stimulation of transcription. Interacts with TEFM. Interacts with MTRES1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the phage and mitochondrial RNA polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="13"/>
    <dbReference type="RefSeq" id="XP_017334298.1">
        <property type="nucleotide sequence ID" value="XM_017478809.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000001156"/>
    <dbReference type="GeneID" id="108271309"/>
    <dbReference type="KEGG" id="ipu:108271309"/>
    <dbReference type="CTD" id="5442"/>
    <dbReference type="OrthoDB" id="276422at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034245">
        <property type="term" value="C:mitochondrial DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001018">
        <property type="term" value="F:mitochondrial promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006390">
        <property type="term" value="P:mitochondrial transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1320.10:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000031">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.280:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1320.10">
        <property type="entry name" value="DNA-directed RNA polymerase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046950">
        <property type="entry name" value="DNA-dir_Rpol_C_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002092">
        <property type="entry name" value="DNA-dir_Rpol_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037159">
        <property type="entry name" value="RNA_POL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029262">
        <property type="entry name" value="RPOL_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00940">
        <property type="entry name" value="RNA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14700">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01311">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00900">
        <property type="entry name" value="RNA_POL_PHAGE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00489">
        <property type="entry name" value="RNA_POL_PHAGE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13 17" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="12">
        <location>
            <begin position="319"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase N-terminal" evidence="15">
        <location>
            <begin position="461"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="109"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="109"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="127"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00063316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017334298.1"/>
        </source>
    </evidence>
    <sequence length="1302" mass="148587" checksum="09DCCE6FCF6D6E2C" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RVA0</accession>
    <name>A0A2D0RVA0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Heat shock factor-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">hsbp1a</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8 9">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Negative regulator of the heat shock response. Negatively affects HSF1 DNA-binding activity. May have a role in the suppression of the activation of the stress response during the aging process.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homohexamer. Associates with heptad repeats of HSF1 trimers and probably also HSF1 monomers, and with HSP70. Association with HSF1 trimers and HSP70 coincides with attenuation of heat shock response and the conversion of HSF1 trimer to monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HSBP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017334427.1">
        <property type="nucleotide sequence ID" value="XM_017478938.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017334428.1">
        <property type="nucleotide sequence ID" value="XM_017478939.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RVA0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006283"/>
    <dbReference type="GeneID" id="108271362"/>
    <dbReference type="KEGG" id="ipu:108271362"/>
    <dbReference type="CTD" id="541432"/>
    <dbReference type="OMA" id="KFQTVSY"/>
    <dbReference type="OrthoDB" id="4159489at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070370">
        <property type="term" value="P:cellular heat acclimation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.430:FF:000002">
        <property type="entry name" value="Heat shock factor-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009643">
        <property type="entry name" value="HS1-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19424">
        <property type="entry name" value="HEAT SHOCK FACTOR BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19424:SF0">
        <property type="entry name" value="HEAT SHOCK FACTOR BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06825">
        <property type="entry name" value="HSBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0346">Stress response</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017334427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017334428.1"/>
        </source>
    </evidence>
    <sequence length="77" mass="8530" checksum="61F0AF5FFA1B1346" modified="2017-12-20" version="1">MSDMEPKSAQDLTVVVQSLLQQMQDKFQTMSDQIIGRIDDMSTRIDDLEKNISDLMTQAGVEEFDGENKMGDASGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RW24</accession>
    <name>A0A2D0RW24_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">N-terminal EF-hand calcium-binding protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">necab3</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017334694.1">
        <property type="nucleotide sequence ID" value="XM_017479205.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RW24"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000036658"/>
    <dbReference type="GeneID" id="108271560"/>
    <dbReference type="KEGG" id="ipu:108271560"/>
    <dbReference type="CTD" id="63941"/>
    <dbReference type="OMA" id="RNNMNKS"/>
    <dbReference type="OrthoDB" id="289247at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000137">
        <property type="term" value="C:Golgi cis cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042984">
        <property type="term" value="P:regulation of amyloid precursor protein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000490">
        <property type="entry name" value="N-terminal EF-hand calcium binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000063">
        <property type="entry name" value="N-terminal EF-hand calcium-binding protein 1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007138">
        <property type="entry name" value="ABM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039862">
        <property type="entry name" value="NECAB1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12178">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12178:SF3">
        <property type="entry name" value="N-TERMINAL EF-HAND CALCIUM-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03992">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51725">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="32"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="ABM" evidence="4">
        <location>
            <begin position="259"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017334694.1"/>
        </source>
    </evidence>
    <sequence length="359" mass="41391" checksum="5E22FD459606CD8D" modified="2017-12-20" version="1">MLACTEMITMCLQSAKRDYLRTEQEQQLQHNHGLSIFHDIFRRADKNDDGKLSFEEFKAYFSDGILTTEELQELFYSIDGRQTNNLDTDKLSDYFSQHLGEYLNVLSALEKLNVAILKAMDKTKEEYQGSSVLGQFVTRFMLKETSSQLLSLQMSLQCAMEAVEEQSSPAPVEVKVPDHLSLQKNNRRCGRRVQKNMCLSPTDPYSGILTTGVTVDTDDHWSSQINRLQQLIDKLECQSPKLEPLKEDSLASTYKSNILLVQRQMAVTEDNLEEFQKALKSYADSTTNQSDNLHVSIQKLPEKSCYIIYEFWQDRISWMSYLQSEASKTFQRSIIDMLEDPELVSTMLMPASWWIMTNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S0M7</accession>
    <name>A0A2D0S0M7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Kelch-like protein 38</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">klhl38a</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SDA103</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047014739.1">
        <property type="nucleotide sequence ID" value="XM_047158783.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S0M7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018739"/>
    <dbReference type="GeneID" id="108272409"/>
    <dbReference type="KEGG" id="ipu:108272409"/>
    <dbReference type="CTD" id="559083"/>
    <dbReference type="OMA" id="EVALTCF"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="CDD" id="cd18476">
        <property type="entry name" value="BACK_KLHL38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056737">
        <property type="entry name" value="Beta-prop_ATRN-MKLN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030568">
        <property type="entry name" value="KLHL38_BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF462">
        <property type="entry name" value="KELCH-LIKE PROTEIN 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24981">
        <property type="entry name" value="Beta-prop_ATRN-LZTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_047014739.1"/>
        </source>
    </evidence>
    <sequence length="583" mass="67110" checksum="CD73F9821ADC8CB5" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S460</accession>
    <name>A0A2D0S460_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Islet cell autoantigen 1-like isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ical1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017337221.2">
        <property type="nucleotide sequence ID" value="XM_017481732.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S460"/>
    <dbReference type="GeneID" id="108272898"/>
    <dbReference type="CTD" id="378720"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051049">
        <property type="term" value="P:regulation of transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07661">
        <property type="entry name" value="BAR_ICA69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000015">
        <property type="entry name" value="Islet cell autoantigen 1, 69kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010504">
        <property type="entry name" value="AH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024114">
        <property type="entry name" value="Islet_autoAg_Ica1/Ica1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006723">
        <property type="entry name" value="Islet_autoAg_Ica1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10164">
        <property type="entry name" value="ISLET CELL AUTOANTIGEN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10164:SF5">
        <property type="entry name" value="ISLET CELL AUTOANTIGEN 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06456">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04629">
        <property type="entry name" value="ICA69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01015">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01237">
        <property type="entry name" value="ICA69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50870">
        <property type="entry name" value="AH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AH" evidence="2">
        <location>
            <begin position="46"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017337221.2"/>
        </source>
    </evidence>
    <sequence length="502" mass="55633" checksum="CDAC6753278CBCD0" modified="2023-06-28" version="2">MDGYGFSSPVLGEDHSVMARMQKKFWKTKQVLIKATGKKEDEHVVASDADLDAKLEFFRSIQTTCTELLKVIEKYQQRITRLSQEENELGLFLRFQAEHDKTKAGSMMNATSKALCTSAKQRLALCAPLQRMEQEMETFRRRAIADTLLTVTRMEKARTEYRGALLWMKDVSQELDPDTYKQLEKFRKVQAQVRGTKVHFDKLNNDVCQKVDMLGASRCNMLSHSLCTYQTTLLQFWEKTANIMSGIHEAFKGYVPYNFTTLKELRDPLEHLLEGPSLEDNKDKEKEMNADKLVSLEEEKTGKSALEAGSLAGFDQDRDSDSSLPASLDSVGRDLLSGLVPHTGPEEEEAERGDLYFLQDLLSPGASSGTDDFSKAWQDAFGCFEAPASAPCSTNAAAASSTAPIRSPSQPPSPTGFLPSQLLDHGISTTGWMTPPMFKTPPLPAPPTPGQVPAAQNSSRMLDNAPKGGARDMSAWFNIFADLDPLSNPDAIGRNDEELLNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S4U3</accession>
    <name>A0A2D0S4U3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein disulfide-isomerase</fullName>
            <ecNumber evidence="13">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">pdia8</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Melanosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="13"/>
    <dbReference type="RefSeq" id="XP_017337773.1">
        <property type="nucleotide sequence ID" value="XM_017482284.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S4U3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000024605"/>
    <dbReference type="GeneID" id="108273190"/>
    <dbReference type="KEGG" id="ipu:108273190"/>
    <dbReference type="CTD" id="445123"/>
    <dbReference type="OrthoDB" id="427280at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02995">
        <property type="entry name" value="PDI_a_PDI_a'_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03073">
        <property type="entry name" value="PDI_b'_ERp72_ERp57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000045">
        <property type="entry name" value="Disulfide-isomerase A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000054">
        <property type="entry name" value="Disulfide-isomerase A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000077">
        <property type="entry name" value="Protein disulfide-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000017">
        <property type="entry name" value="Protein disulfide-isomerase A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005788">
        <property type="entry name" value="PDI_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005792">
        <property type="entry name" value="Prot_disulphide_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01130">
        <property type="entry name" value="ER_PDI_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01126">
        <property type="entry name" value="pdi_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929:SF45">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13848">
        <property type="entry name" value="Thioredoxin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 13 16" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10 11" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5011816501" description="Protein disulfide-isomerase" evidence="13">
        <location>
            <begin position="25"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="14">
        <location>
            <begin position="21"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="14">
        <location>
            <begin position="357"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="11">
        <location>
            <begin position="56"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="11">
        <location>
            <begin position="404"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605792-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017337773.1"/>
        </source>
    </evidence>
    <sequence length="495" mass="56241" checksum="A824ECD16C50830B" modified="2017-12-20" version="1">MADFVSRLLLLCVFSAVVWSSVLAARSDVMELKDLDFDYIAAEQETMLVKFYAPWCGHCKKLAPEFESAAARLKGIATLAKVDCTSNTEVCGRFGVTGYPTLKLFKNGVETSTYDGPRSSDGIVDQVKKQAGPDSMLLNSEKDLEDFISHFDPSVVGVFESSDSPQLVEFLKGANLMRDSFRFAHTTDMQLGLKYELTAEGVLLFRPPRLASKFEDSVVRHRGSFSITGLRRFIRDNIYGICPHLTKENKDQLRKRDLLTAYYDLDYRRNPKGSNYWRNRVLKVASQYKDHGLHFSVANRQDFLDELEEEYGLGTTEGSDMPFVTVRTRLGHKYTMREEFTRDGKSLERFLEDYLAGRLKRYVKSEPVPTNNNGPVKVVVADTFEEIVNDPEKDVLIEFYAPWCGHCKKLEPKYTELGQELSSDPSIVIAKMDATANDVPQGYDVEGFPTIYFVPALRKNEPRRYEGGREVKDFLSFLKREATSPLVLKGVKEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S8A6</accession>
    <name>A0A2D0S8A6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Catechol O-methyltransferase domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC108273856</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017338978.1">
        <property type="nucleotide sequence ID" value="XM_017483489.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S8A6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000002081"/>
    <dbReference type="GeneID" id="108273856"/>
    <dbReference type="KEGG" id="ipu:108273856"/>
    <dbReference type="OMA" id="VCFEGVF"/>
    <dbReference type="OrthoDB" id="10251242at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050362">
        <property type="entry name" value="Cation-dep_OMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002935">
        <property type="entry name" value="SAM_O-MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10509:SF93">
        <property type="entry name" value="CATECHOL O-METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10509">
        <property type="entry name" value="O-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01596">
        <property type="entry name" value="Methyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51682">
        <property type="entry name" value="SAM_OMT_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017338978.1"/>
        </source>
    </evidence>
    <sequence length="259" mass="28968" checksum="EF73FD1EE3AC85C7" modified="2017-12-20" version="1">MLIHAVSKEALISSATLGVVFITVYYIRKHRSVRFSMSNFKSHVAGKDDPVMQYVLNHSLREHPALKKLRLKTMEHRGNPMMVACEQSQFMANLARLIKAKKAIEIGVYTGYNTLSIALALPDDGKVVACDVSEEYADIGKPFWKEAGVEHRIDLRVQPALKTLDDLLAAGEAETFDFVFIDADKQNYDNYYEKSLQLVRKGGIIAIDNVLWSGRVLSPAEDDVDTQSIATLNKKLHRDVRVNLSMLTVGDGLSLAFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SBU4</accession>
    <name>A0A2D0SBU4_ICTPU</name>
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        <submittedName>
            <fullName evidence="5">Cilia- and flagella- associated protein 210</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cfap210</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017340204.1">
        <property type="nucleotide sequence ID" value="XM_017484715.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SBU4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034459"/>
    <dbReference type="GeneID" id="108274510"/>
    <dbReference type="KEGG" id="ipu:108274510"/>
    <dbReference type="CTD" id="129881"/>
    <dbReference type="OMA" id="EMHFRSQ"/>
    <dbReference type="OrthoDB" id="331765at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005879">
        <property type="term" value="C:axonemal microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039986">
        <property type="entry name" value="CFAP210"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043597">
        <property type="entry name" value="TPH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28663:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA- ASSOCIATED PROTEIN 210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28663">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13868">
        <property type="entry name" value="TPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0969">Cilium</keyword>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trichohyalin-plectin-homology" evidence="3">
        <location>
            <begin position="139"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="89"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="204"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017340204.1"/>
        </source>
    </evidence>
    <sequence length="555" mass="64800" checksum="267FCF8F7FECEC0F" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SBY2</accession>
    <name>A0A2D0SBY2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">guanylate cyclase</fullName>
            <ecNumber evidence="2">4.6.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC108274535</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="4.6.1.2" evidence="2"/>
    <dbReference type="RefSeq" id="XP_047015955.1">
        <property type="nucleotide sequence ID" value="XM_047159999.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SBY2"/>
    <dbReference type="GeneID" id="108274535"/>
    <dbReference type="KEGG" id="ipu:108274535"/>
    <dbReference type="OrthoDB" id="6127067at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008074">
        <property type="term" value="C:guanylate cyclase complex, soluble"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004383">
        <property type="term" value="F:guanylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038060">
        <property type="term" value="P:nitric oxide-cGMP-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070482">
        <property type="term" value="P:response to oxygen levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000007">
        <property type="entry name" value="Guanylate cyclase soluble subunit alpha-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1520.10">
        <property type="entry name" value="H-NOX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.260">
        <property type="entry name" value="Haem NO binding associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038158">
        <property type="entry name" value="H-NOX_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011644">
        <property type="entry name" value="Heme_NO-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011645">
        <property type="entry name" value="HNOB_dom_associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042463">
        <property type="entry name" value="HNOB_dom_associated_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45655">
        <property type="entry name" value="GUANYLATE CYCLASE SOLUBLE SUBUNIT BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45655:SF10">
        <property type="entry name" value="SOLUBLE GUANYLATE CYCLASE 88E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07700">
        <property type="entry name" value="HNOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07701">
        <property type="entry name" value="HNOBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0141">cGMP biosynthesis</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="691"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="701"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="719"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_047015955.1"/>
        </source>
    </evidence>
    <sequence length="756" mass="85840" checksum="5AFDC2553260A9A5" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SEA8</accession>
    <name>A0A2D0SEA8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Coiled-coil domain-containing protein 146 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ccdc146</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017341054.2">
        <property type="nucleotide sequence ID" value="XM_017485565.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SEA8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026513"/>
    <dbReference type="GeneID" id="108275026"/>
    <dbReference type="KEGG" id="ipu:108275026"/>
    <dbReference type="CTD" id="57639"/>
    <dbReference type="OrthoDB" id="10262929at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32083">
        <property type="entry name" value="CILIA AND FLAGELLA-ASSOCIATED PROTEIN 58-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32083:SF34">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="908"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="88"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="185"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="401"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017341054.2"/>
        </source>
    </evidence>
    <sequence length="948" mass="110655" checksum="185DDDE99BFE7151" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SEU2</accession>
    <name>A0A2D0SEU2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">diphosphoinositol-polyphosphate diphosphatase</fullName>
            <ecNumber evidence="5">3.6.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">nudt4b</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>diphospho-myo-inositol polyphosphate + H2O = myo-inositol polyphosphate + phosphate.</text>
            <dbReference type="EC" id="3.6.1.52"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Nudix hydrolase family. DIPP subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.52" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017341242.1">
        <property type="nucleotide sequence ID" value="XM_017485753.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SEU2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000023981"/>
    <dbReference type="GeneID" id="108275142"/>
    <dbReference type="KEGG" id="ipu:108275142"/>
    <dbReference type="CTD" id="440672"/>
    <dbReference type="OMA" id="HRTQVWV"/>
    <dbReference type="OrthoDB" id="2011998at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034431">
        <property type="term" value="F:bis(5'-adenosyl)-hexaphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034432">
        <property type="term" value="F:bis(5'-adenosyl)-pentaphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008486">
        <property type="term" value="F:diphosphoinositol-polyphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000298">
        <property type="term" value="F:endopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901911">
        <property type="term" value="P:adenosine 5'-(hexahydrogen pentaphosphate) catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901909">
        <property type="term" value="P:diadenosine hexaphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901907">
        <property type="term" value="P:diadenosine pentaphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071543">
        <property type="term" value="P:diphosphoinositol polyphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04666">
        <property type="entry name" value="NUDIX_DIPP2_like_Nudt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000002">
        <property type="entry name" value="diphosphoinositol polyphosphate phosphohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047198">
        <property type="entry name" value="DDP-like_NUDIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12629">
        <property type="entry name" value="DIPHOSPHOINOSITOL POLYPHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12629:SF6">
        <property type="entry name" value="DIPHOSPHOINOSITOL POLYPHOSPHATE PHOSPHOHYDROLASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="12">
        <location>
            <begin position="16"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017341242.1"/>
        </source>
    </evidence>
    <sequence length="173" mass="19644" checksum="E1A74000239CCB41" modified="2017-12-20" version="1">MKFKPNQTRTYDGEGFKQRAACLCFRNEREDEVLLVSSSRHPDQWIVPGGGMEPEEEPCGAAVREVYEEAGVKGKLGRLLGVFEHNQDRKHRTHVYVLTVTETLEAWEDSVNIGRKREWFPVEEAIKILQSHKPVHAEYLRHLQLSCSTTNNGNILLPPPPPNDNTARAAACR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SF53</accession>
    <name>A0A2D0SF53_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">L-lactate dehydrogenase</fullName>
            <ecNumber evidence="5 11">1.1.1.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ldhbb</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(S)-lactate + NAD(+) = pyruvate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23444"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16651"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LDH/MDH superfamily. LDH family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.27" evidence="5 11"/>
    <dbReference type="RefSeq" id="XP_017341101.1">
        <property type="nucleotide sequence ID" value="XM_017485612.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SF53"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034378"/>
    <dbReference type="GeneID" id="108275055"/>
    <dbReference type="KEGG" id="ipu:108275055"/>
    <dbReference type="CTD" id="436747"/>
    <dbReference type="OMA" id="DICHNIL"/>
    <dbReference type="OrthoDB" id="5405561at2759"/>
    <dbReference type="UniPathway" id="UPA00554">
        <property type="reaction ID" value="UER00611"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006089">
        <property type="term" value="P:lactate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05293">
        <property type="entry name" value="LDH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000029">
        <property type="entry name" value="L-lactate dehydrogenase A chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.110.10:FF:000003">
        <property type="entry name" value="L-lactate dehydrogenase A chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00488">
        <property type="entry name" value="Lactate_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011304">
        <property type="entry name" value="L-lactate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018177">
        <property type="entry name" value="L-lactate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01771">
        <property type="entry name" value="L-LDH-NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000824">
        <property type="entry name" value="PRK00066.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128">
        <property type="entry name" value="L-2-HYDROXYCARBOXYLATE DEHYDROGENASE (NAD(P)(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128:SF2">
        <property type="entry name" value="L-LACTATE DEHYDROGENASE B CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00064">
        <property type="entry name" value="L_LDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10 11" id="KW-0520">NAD</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="12">
        <location>
            <begin position="44"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="13">
        <location>
            <begin position="185"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="49"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="158"/>
            <end position="160"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017341101.1"/>
        </source>
    </evidence>
    <sequence length="355" mass="39405" checksum="9553436DB7CBBBDB" modified="2017-12-20" version="1">MLISWWYLYLGAHSIYKQVQNMASVLQKLITPLFNGPVEPPRNKVTVVGVGQVGMACAVSILLQELADELALVDVMEDKLKGEMLDLQHGSLFFKTPKIVSGKDYSVTANSRIVVVTAGVRQQEGESRLNLVQRNINVFQHIIPQIIKYSPNCILIVVSNPVDVLTYVTWKLSGLPTHRVIGSGTNLDSARFRYLMAERLGIHPSSFNGWILGEHGDSSVPVWSGANVAGVNLQKLNPDIGQDSDRENWKKTHKMVVDSAYEVIRLKGYTNWAIGLSVADLTESLIKNLNRVHPISTMVKGMYGITDHVFLSLPCVLNSRGVSSVVNMNLTQDEVSQLKKSADTLWNIQKDLKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SFD5</accession>
    <name>A0A2D0SFD5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Enoyl-CoA hydratase domain-containing protein 3, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">echdc3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May play a role in fatty acid biosynthesis and insulin sensitivity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017340955.1">
        <property type="nucleotide sequence ID" value="XM_017485466.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SFD5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022866"/>
    <dbReference type="GeneID" id="108274980"/>
    <dbReference type="KEGG" id="ipu:108274980"/>
    <dbReference type="CTD" id="79746"/>
    <dbReference type="OMA" id="SCDMVVC"/>
    <dbReference type="OrthoDB" id="2139957at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052377">
        <property type="entry name" value="Mitochondrial_ECH-domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006008">
        <property type="entry name" value="PRK08139.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43602">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43602:SF1">
        <property type="entry name" value="ENOYL-COA HYDRATASE DOMAIN-CONTAINING PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017340955.1"/>
        </source>
    </evidence>
    <sequence length="288" mass="31361" checksum="53E470BD24AFBCBA" modified="2017-12-20" version="1">MSQVVFSRFSSFCRLSARRFCTQATSPLTIREQEDGIRRIILNNPKKRNALSLAMLTSLQENILSGIDDKDLRVIIIAAQGPVFSSGHDLKELTSVQGRDYHTRVFRVCSEVMTLIQDLPVPVIAMVNGIATAAGCQLVASCDIAVATDKSSFATPGVNVGLFCSTPGVAIGRAVPRKVAMEMLFTGRPITAQEALLHGLVSKVVREECLEEETLAIARRICDSSRPVVALGKAAFQRQMAQARDAAYATATAVMVDNLALRDGQEGIQAFLEKRRPLWTNSTEKAHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SH38</accession>
    <name>A0A2D0SH38_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Class E basic helix-loop-helix protein 23</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">bhlhe23</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017342027.1">
        <property type="nucleotide sequence ID" value="XM_017486538.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SH38"/>
    <dbReference type="GeneID" id="108275634"/>
    <dbReference type="KEGG" id="ipu:108275634"/>
    <dbReference type="CTD" id="128408"/>
    <dbReference type="OrthoDB" id="10011855at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070888">
        <property type="term" value="F:E-box binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000026">
        <property type="entry name" value="Basic helix-loop-helix family, member e23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050359">
        <property type="entry name" value="bHLH_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290:SF53">
        <property type="entry name" value="CLASS E BASIC HELIX-LOOP-HELIX PROTEIN 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="104"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="58"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="89"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017342027.1"/>
        </source>
    </evidence>
    <sequence length="228" mass="24466" checksum="B5F77F71F0A97047" modified="2017-12-20" version="1">MNAREENLLKSISNDTLLDLTQRYGQAALGFGAGHGASSPARFPLASSAAADFLTAQTAKSGESGGEHTSDDDDSFDVDARKQSSAFGEDPKHPAAKKPKEQRSLRLSINARERRRMHDLNDALDGLRAVIPYAHSPSVRKLSKIATLLLAKNYILMQAQALEEMRRLVAYLNQGQTLTSAVPSALAPFGQTPATVYPFSGTALAACVEKCSYSAAPSSLFKHCSDKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SI67</accession>
    <name>A0A2D0SI67_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Baculoviral IAP repeat-containing protein 5b isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">birc5b</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the IAP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017341975.1">
        <property type="nucleotide sequence ID" value="XM_017486486.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SI67"/>
    <dbReference type="GeneID" id="108275580"/>
    <dbReference type="KEGG" id="ipu:108275580"/>
    <dbReference type="CTD" id="246726"/>
    <dbReference type="OrthoDB" id="2196114at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00022">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1170.10:FF:000009">
        <property type="entry name" value="Baculoviral IAP repeat-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051190">
        <property type="entry name" value="Baculoviral_IAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001370">
        <property type="entry name" value="BIR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46771:SF2">
        <property type="entry name" value="BACULOVIRAL IAP REPEAT-CONTAINING PROTEIN 5.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46771">
        <property type="entry name" value="DETERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00653">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00238">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50143">
        <property type="entry name" value="BIR_REPEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="17" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017341975.1"/>
        </source>
    </evidence>
    <sequence length="194" mass="22563" checksum="7F50DF209E8139D0" modified="2017-12-20" version="1">MNYSDVIAARFLTFDQMYSYEKRLQTFADWPFREDCQCTPEQMAKAGFVHCPSENEPDVACCFYCLKELEGWEPEDIPWFEHAKRSPDCGFLQMKSPDELTAVDFFRLEHGRMVNYLRKMAHMKIAYFRDQVDVTSRNLRLLFEIFRPHGGSVMQGSSLDPCNVMRGALNPSSICSAFPQILTKSCRRLTELNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SJQ0</accession>
    <name>A0A2D0SJQ0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">mitogen-activated protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">zgc:171775</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017342933.1">
        <property type="nucleotide sequence ID" value="XM_017487444.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SJQ0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009827"/>
    <dbReference type="GeneID" id="108276094"/>
    <dbReference type="KEGG" id="ipu:108276094"/>
    <dbReference type="OMA" id="YIVTDCM"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07851">
        <property type="entry name" value="STKc_p38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000684">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000769">
        <property type="entry name" value="Mitogen-activated protein kinase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008352">
        <property type="entry name" value="MAPK_HOG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01773">
        <property type="entry name" value="P38MAPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="12" id="KW-0346">Stress response</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="25"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017342933.1"/>
        </source>
    </evidence>
    <sequence length="359" mass="41378" checksum="F012B16490E668E7" modified="2017-12-20" version="1">MASPLRAGFHRLEVQKTSWEIPDRYTALNPVGSGAYGTVCSAIDQKTKEKVAIKKLYRPFQSLIHAKRAYRELRLLRHIQHDNVICLLDVFTPDSSLDKFQTFYLVMPFVAQDLGHIMKRRRLTEKVIVYLFYQLLRGLKYIHSAGIIHRDLKPNNLAVDENCELKILDFGLARHAEMEMTGYVVTRWYRAPEVIFNWMHYTQTVDIWSAGCILAEMITGEVLFPGNDSMDQLNMILRLTGTPHSSLVQKMQSKDARSYVQSLPVQKKKVLRDVFPSMDSKAVNLLEKTLMLDPEVRLTAKDGLTHPYLSDFHDPENEPESPSYDDSFESLELAVSEWKSLIHMEIMTFDPDNPRQTAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SN00</accession>
    <name>A0A2D0SN00_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein si:ch211-198p11.6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">si:ch211-198p11.6</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017344082.1">
        <property type="nucleotide sequence ID" value="XM_017488593.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SN00"/>
    <dbReference type="GeneID" id="108276725"/>
    <dbReference type="KEGG" id="ipu:108276725"/>
    <dbReference type="OrthoDB" id="6365775at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017344082.1"/>
        </source>
    </evidence>
    <sequence length="114" mass="12667" checksum="56DB491508D78812" modified="2017-12-20" version="1">MMTVVPLWDLSLPLPAVVLISVGLYMLFLAGVLWCHHCLKTQCNPQCTNCCPSVSPFEYCHMCAQSCDCHPPSLHTCLDHSCPSPNCAMWDCTCNPQPPQCDSINCLCCEIKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SNJ7</accession>
    <name>A0A2D0SNJ7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coagulation factor IX</fullName>
            <ecNumber evidence="4">3.4.21.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Christmas factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="primary">f9b</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30">SDA103</strain>
        </source>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="31">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Selective cleavage of Arg-|-Ile bond in factor X to form factor Xa.</text>
            <dbReference type="EC" id="3.4.21.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="25">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.22" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017344279.1">
        <property type="nucleotide sequence ID" value="XM_017488790.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SNJ7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026283"/>
    <dbReference type="GeneID" id="108276806"/>
    <dbReference type="KEGG" id="ipu:108276806"/>
    <dbReference type="CTD" id="678552"/>
    <dbReference type="OMA" id="SYECWCR"/>
    <dbReference type="OrthoDB" id="8909918at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000162">
        <property type="entry name" value="Coagulation factor X (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000120">
        <property type="entry name" value="Putative serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.740.10:FF:000001">
        <property type="entry name" value="vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.740.10">
        <property type="entry name" value="Coagulation Factor IX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017857">
        <property type="entry name" value="Coagulation_fac-like_Gla_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035972">
        <property type="entry name" value="GLA-like_dom_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000294">
        <property type="entry name" value="GLA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012224">
        <property type="entry name" value="Pept_S1A_FX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050442">
        <property type="entry name" value="Peptidase_S1_coag_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278">
        <property type="entry name" value="COAGULATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278:SF31">
        <property type="entry name" value="COAGULATION FACTOR IX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00594">
        <property type="entry name" value="Gla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001143">
        <property type="entry name" value="Factor_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00001">
        <property type="entry name" value="GLABLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00069">
        <property type="entry name" value="GLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57630">
        <property type="entry name" value="GLA-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00011">
        <property type="entry name" value="GLA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50998">
        <property type="entry name" value="GLA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="18" id="KW-0094">Blood coagulation</keyword>
    <keyword evidence="16" id="KW-0106">Calcium</keyword>
    <keyword evidence="20 25" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 25" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6" id="KW-0301">Gamma-carboxyglutamic acid</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0356">Hemostasis</keyword>
    <keyword evidence="14 26" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="22" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10 26" id="KW-0645">Protease</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="15 26" id="KW-0720">Serine protease</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="19" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Gla" evidence="29">
        <location>
            <begin position="45"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="27">
        <location>
            <begin position="91"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="28">
        <location>
            <begin position="260"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="24">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="24">
        <location>
            <position position="351"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="24">
        <location>
            <position position="446"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00031357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR001143-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="RefSeq" id="XP_017344279.1"/>
        </source>
    </evidence>
    <sequence length="505" mass="56390" checksum="AEC44FB83B6A3295" modified="2017-12-20" version="1">MEKISLLALIYGIFILISNLCLTSSVSVFVSRQMAQSVLSRQKRFNKGALEEVVRDNLERECIEEKCNFEEAREVFENVEKTREFWIGYIDGDQCLSSPCQNGGTCKDGLSSYACWCPIGFNGKNCELELVRQCDVNNGGCMQFCVVDKIRGVVCNCADGYTLGADERSCEPKDNYPCGRLGKKIANTLSTRTLITEETVIQIHAIKHVNVTERNLTAANSTNSTELSSATIRPTPGENYWDFFPTLPTVIKETNTDQRIVGGLDATPGEIPWQVALVHKEKKVVFCGGSLLSDMWVITAAHCLVEGGIGEFFIRLGEHNVKIDEKRESDHEIDEHHVHPNYQYQQSHNHDIALLKLKTPATFSDYIIPICLGPKTFTENLLKSATLSLVSGWGRIRYGGLESDTLQKVEVPYVDRTECKGSDKISRFMFCAGFLTTRKDSCQGDSGGPHATRLGTNTWFLTGVISWGDECAKEGKYGIYTRVSRYMNWITNMTGIRAGILNDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SNL4</accession>
    <name>A0A2D0SNL4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">RNA-binding Raly-like protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC108276674</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRM HNRPC family. RALY subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017344091.1">
        <property type="nucleotide sequence ID" value="XM_017488602.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SNL4"/>
    <dbReference type="GeneID" id="108276674"/>
    <dbReference type="KEGG" id="ipu:108276674"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000019">
        <property type="entry name" value="heterogeneous nuclear ribonucleoproteins C1/C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017347">
        <property type="entry name" value="hnRNP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051186">
        <property type="entry name" value="RRM_HNRPC/RALY_subfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13968">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13968:SF21">
        <property type="entry name" value="RNA-BINDING RALY-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037992">
        <property type="entry name" value="hnRNP-C_Raly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="21"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="154"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="246"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="160"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="246"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="301"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017344091.1"/>
        </source>
    </evidence>
    <sequence length="310" mass="34315" checksum="169A9701A40E6566" modified="2017-12-20" version="1">MTGKSQTSNVTNKNDPRSLNSRVFIGNLNTAIVKKADIEVIFAKYGKIVGCSVHKGYAFVQYMSERNARAAVAGENARIIAGQPLDINMAGEPKPYRPKAGSKRPLSAVYSGYEFDYDYYREDFYNRLFDYHGRVAPPPRAVIPLKRSRVVVPSTRRGKTSFSIKTSTSSSSRPPSSSSSSFSSGLKPFQMEGSGTRVIPDGREIMKTDQLQTIKRELTQIKLKIDSLLGRLEKIEKQQRAEAEAQRKYEDNCDSLHEESVSETAENSAEEAGEGTLDAEAGEMTDGGEDDYDEEGSTDLIENHVSDIDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SNS2</accession>
    <name>A0A2D0SNS2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Integral membrane protein DGCR2/IDD isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">dgcr2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017344352.1">
        <property type="nucleotide sequence ID" value="XM_017488863.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SNS2"/>
    <dbReference type="GeneID" id="108276840"/>
    <dbReference type="CTD" id="9993"/>
    <dbReference type="OrthoDB" id="8998425at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042378">
        <property type="entry name" value="IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15256">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN DGCR2/IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15256:SF6">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN DGCR2_IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5011977069" evidence="5">
        <location>
            <begin position="29"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="6">
        <location>
            <begin position="121"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="468"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="513"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="64"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017344352.1"/>
        </source>
    </evidence>
    <sequence length="526" mass="58859" checksum="26FF3BD70186152E" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0STN9</accession>
    <name>A0A2D0STN9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">rhomboid protease</fullName>
            <ecNumber evidence="4">3.4.21.105</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC108277433</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.</text>
            <dbReference type="EC" id="3.4.21.105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017345675.1">
        <property type="nucleotide sequence ID" value="XM_017490186.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0STN9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000002729"/>
    <dbReference type="GeneID" id="108277433"/>
    <dbReference type="KEGG" id="ipu:108277433"/>
    <dbReference type="CTD" id="792889"/>
    <dbReference type="OMA" id="WVKQELW"/>
    <dbReference type="OrthoDB" id="10260614at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000005">
        <property type="entry name" value="Presenilins-associated rhomboid-like protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050925">
        <property type="entry name" value="Rhomboid_protease_S54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731:SF29">
        <property type="entry name" value="PRESENILINS-ASSOCIATED RHOMBOID-LIKE PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731">
        <property type="entry name" value="RHOMBOID PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="222"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="13">
        <location>
            <begin position="183"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017345675.1"/>
        </source>
    </evidence>
    <sequence length="358" mass="40175" checksum="A8A5B67BC9688AD2" modified="2017-12-20" version="1">MAWRGCVIRWVKEDFISQASRRNGFILHQQRCFRKGVKKVEPKMEAEQAELIQKKGGTPAIEPPAPSQPERSLRRLVRPMVFTIGFTGCSFGTAAIWQYESLKSRVQSYFDEVRADWLDKLRPQKHGDLRKQINQWWNSLSEGQRSVTGIIAANAVVFCCWRIPSLQRSMLKYFTSNPTSSARCWPMLLSSFSHYSFFHMAANMYVLWSFSSSAVSMMGKEQFVAVYLSAGVISSFASYVCKTLTGRMGPSLGASGAIMTVLAAVCTKMPEAKLAIVFLPMYTFTAGNALKAIVALDTAGLIMGWRFFDHAAHLGGALFGIWYIIYGNELIWKNREPLVKMWHNFRTRGPGTGGNGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SVN8</accession>
    <name>A0A2D0SVN8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC108278103</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017346748.1">
        <property type="nucleotide sequence ID" value="XM_017491259.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SVN8"/>
    <dbReference type="GeneID" id="108278103"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14126">
        <property type="entry name" value="STKc_CK1_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001113">
        <property type="entry name" value="Casein kinase 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000018">
        <property type="entry name" value="Casein kinase I isoform gamma-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022247">
        <property type="entry name" value="Casein_kinase-1_gamma_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12605">
        <property type="entry name" value="CK1gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="51"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="345"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="403"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="351"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017346748.1"/>
        </source>
    </evidence>
    <sequence length="454" mass="51235" checksum="B1100AD07A184FF0" modified="2017-12-20" version="1">MDFDKRGGGGGGGKGDTEEGKKMSKTGGSRSGHTGRTSAPNSGVLMVGPNFRVGKKIGCGNFGELRLGKNLYTNEYVAIKLEPIKSRAPQLHLEYRFYKQLGTSEGVPQVYYFGPCGKYNAMVLELLGPSLEDLFDLCDRTFSLKTVLMIAIQLITRMEYVHTRSLIYRDVKPENFLVGRPGSKRQHTIHIIDFGLAKEYIDPETKKHIPYREHKSLTGTARYMSINTHLGKEQSRRDDLEALGHMFMYFLRGSLPWQGLKADTLKERYQKIGDTKRATPIEVLCESFPEEMATYLRYVRRLDFFEKPDYDYLRKLFTDLFDRNGYVFDYEYDWVGKTLPTPIGPIPTDAPIQSGSRDKAQQQTKNQSPEPKGSESQPTPVTNRELLGSHLTADRLGGSVQVMSSTNGEVNTDDPTAGHSNAPITAPAEVEVTDDTKCCCFFKRRKRKGLQRHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T6S5</accession>
    <name>A0A2D0T6S5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Anti-dorsalizing morphogenic protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">admp</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017350597.1">
        <property type="nucleotide sequence ID" value="XM_017495108.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T6S5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000024797"/>
    <dbReference type="GeneID" id="108280256"/>
    <dbReference type="KEGG" id="ipu:108280256"/>
    <dbReference type="CTD" id="140619"/>
    <dbReference type="OMA" id="VFGMDDP"/>
    <dbReference type="OrthoDB" id="5987191at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072359">
        <property type="term" value="P:circulatory system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035239">
        <property type="term" value="P:tube morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13765">
        <property type="entry name" value="TGF_beta_ADMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.970:FF:000023">
        <property type="entry name" value="Anti-dorsalizing morphogenetic protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000001">
        <property type="entry name" value="Bone morphogenetic protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF241">
        <property type="entry name" value="ANTI-DORSALIZING MORPHOTIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0339">Growth factor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012790839" evidence="9">
        <location>
            <begin position="19"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="10">
        <location>
            <begin position="277"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017350597.1"/>
        </source>
    </evidence>
    <sequence length="395" mass="44262" checksum="5B88A7AE81A3B3D5" modified="2017-12-20" version="1">MFSAVVFFLVSIAGLGNSRPSIDYSENRFPVENDSEEVRSAAIKRLLEVFGMEDPPASLSRGHKQPPQYMLDLYNTVADVDGVTKDPTLLEGNTVRSFFDKLHSQQVEFMFNLSTVARNEKVLTAELHLFKLRPQASLIFNRHHFCQVSVYQRLDNTKMNVSQGKKLVSSRLIPVHSNGWEVFTITQAVRTWMTDEHSNLGLLVTVRTVADVQLDVRNVRFASGQNHHHSKQPMLVLFTDDGRRVTSPEGALKGSDDSLAGAKLPLPSLILPGPGHRSARSLDYDEDPDRKPCQRLPLYVDFEEIGWSGWIVSPRGYNAYHCKGSCPFPLGQNMRPTNHATVQSIINALKLTKGIDTPCCVPDKLFSINLLYFDDDENVVLKQYDDMVAGSCGCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T966</accession>
    <name>A0A2D0T966_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein fuzzy homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">fuz</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fuzzy family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017351418.1">
        <property type="nucleotide sequence ID" value="XM_017495929.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T966"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000014912"/>
    <dbReference type="GeneID" id="108280658"/>
    <dbReference type="KEGG" id="ipu:108280658"/>
    <dbReference type="CTD" id="80199"/>
    <dbReference type="OMA" id="LDQYSCS"/>
    <dbReference type="OrthoDB" id="74835at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043972">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043971">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043970">
        <property type="entry name" value="FUZ/MON1/HPS1_longin_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026069">
        <property type="entry name" value="Fuzzy"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13559">
        <property type="entry name" value="INTRACELLULAR TRAFFIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13559:SF1">
        <property type="entry name" value="PROTEIN FUZZY HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19036">
        <property type="entry name" value="Fuz_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19037">
        <property type="entry name" value="Fuz_longin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19038">
        <property type="entry name" value="Fuz_longin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FUZ/MON1/HPS1 first Longin" evidence="5">
        <location>
            <begin position="17"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="FUZ/MON1/HPS1 second Longin" evidence="6">
        <location>
            <begin position="185"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="FUZ/MON1/HPS1 third Longin" evidence="7">
        <location>
            <begin position="306"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF19036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF19037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF19038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017351418.1"/>
        </source>
    </evidence>
    <sequence length="435" mass="48222" checksum="BA2E54C860E5DA72" modified="2017-12-20" version="1">MFSIPSSLGMLHSQSVQLLCLTASSGVPLFCRGSTKQLPFSIIGSLNGVHMFGTGHDAALSCCETERGGRVLWRVFHDSLMLIGISSNADVSELHLRRLLENAWNCMVLVLGLDELANTRNVERLKRELRSCYRLIDMLLERGMDDRDGDRGLMGDLTHCADCILFPQADLLQETLDAFTRAAESEFGCLLVRGRLAVATEKWWRLAPQELVLLSALANLPPGKTSCDCPVFLPQGSPNVALRLLCFQLLPGAIACVLCGPRPSLYHVENEQVCRFWSPVVENLRSCLDQAKRSALSPSVGLRRDVLALLLINRESRRSVESLGHACSKATPLLSPARRWELLRLFYTFAATRYFSSEESPSSERPTAELLHEEFSAGFTHLPVHCYLVTDECKCYAVQTPQHQLFLLTDLSVPTFALRSVATHTLDAVTTATGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979E4V1</accession>
    <name>A0A979E4V1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Trichohyalin isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC108260022</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047007987.1">
        <property type="nucleotide sequence ID" value="XM_047152031.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979E4V1"/>
    <dbReference type="GeneID" id="108260022"/>
    <dbReference type="KEGG" id="ipu:108260022"/>
    <dbReference type="OrthoDB" id="8951333at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002274">
        <property type="entry name" value="Si:dkeyp-69e1.8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF167">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="6">
        <location>
            <begin position="428"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="359"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="441"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="690"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="787"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="458"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_047007987.1"/>
        </source>
    </evidence>
    <sequence length="821" mass="96892" checksum="A3BD34523FBCF99B" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EWA6</accession>
    <name>A0A979EWA6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Low-density lipoprotein receptor-related protein 1B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">lrp1bb</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Membrane</location>
            <location evidence="15">Coated pit</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LDLR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047012062.2">
        <property type="nucleotide sequence ID" value="XM_047156106.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108266785"/>
    <dbReference type="CTD" id="559293"/>
    <dbReference type="OrthoDB" id="10066840at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043226">
        <property type="term" value="C:organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005041">
        <property type="term" value="F:low-density lipoprotein particle receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="30"/>
    </dbReference>
    <dbReference type="CDD" id="cd12087">
        <property type="entry name" value="TM_EGFR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000022">
        <property type="entry name" value="LDL receptor related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000012">
        <property type="entry name" value="Low density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000007">
        <property type="entry name" value="Low density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000010">
        <property type="entry name" value="Low density lipoprotein receptor-related protein 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000009">
        <property type="entry name" value="Low-density lipoprotein receptor isoform 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000024">
        <property type="entry name" value="Low-density lipoprotein RecePtor related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000129">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000505">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000014">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000018">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000019">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000002">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000004">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000011">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000012">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000015">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000018">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000072">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000498">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000005">
        <property type="entry name" value="low-density lipoprotein receptor-related protein 1B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000061">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000070">
        <property type="entry name" value="low-density lipoprotein receptor-related protein 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000073">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000080">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000110">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000241">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000088">
        <property type="entry name" value="Prolow-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000020">
        <property type="entry name" value="Prolow-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000009">
        <property type="entry name" value="Putative low-density lipoprotein receptor-related protein 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000065">
        <property type="entry name" value="Transmembrane protease serine 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="32"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056588">
        <property type="entry name" value="EGF_LRP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051221">
        <property type="entry name" value="LDLR-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722:SF5">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24468">
        <property type="entry name" value="EGF_LRP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="30"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="32"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="32"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="20"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="13" id="KW-0168">Coated pit</keyword>
    <keyword evidence="11 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 16" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="23" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 23" id="KW-0675">Receptor</keyword>
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</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EWS3</accession>
    <name>A0A979EWS3_ICTPU</name>
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        <submittedName>
            <fullName evidence="12">Uncharacterized protein LOC108266668</fullName>
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        <name evidence="12" type="primary">LOC108266668</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047012298.1">
        <property type="nucleotide sequence ID" value="XM_047156342.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EWS3"/>
    <dbReference type="GeneID" id="108266668"/>
    <dbReference type="KEGG" id="ipu:108266668"/>
    <dbReference type="CTD" id="220416"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040643">
        <property type="entry name" value="MLVIN_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF46">
        <property type="entry name" value="LEUCINE RICH REPEAT-CONTAINING DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18697">
        <property type="entry name" value="MLVIN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="230"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Murine leukemia virus integrase C-terminal" evidence="9">
        <location>
            <begin position="382"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="353"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="445"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="353"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="445"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="461"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_047012298.1"/>
        </source>
    </evidence>
    <sequence length="474" mass="53797" checksum="ACAD9A444E9A8D47" modified="2023-02-22" version="1">MLPVFLLGEIAESIPAQVQEEGTERRNEWSDHGNALPWNNGLPSSHLSFLPAETPIVPYKIINSFPSRPRRSIPKARISDVLRDDPRYTVQPPNFLLEFLKPTQSESEPDQTSQSQKKVVQYLSGYNYRKLVDHLLSELHDGQQREVASQILSCEQLPQLERRIPQRQIICVVLSFQGSCRSHSPSLAPVSPSERAVVECLKKGGATLNLKAHFIDQLPDLSLLISTICYLNLSFNDFSLFPVEVCELTKLEVLKLRDNPIEEIPAEIQKLINLKMLVVSFCKLTALPSKLYQLPSLQHLDVSHNLIGQLSNSISNLRSLRYLNIEGNQLAALPAGLLRLLLSQLKLDRSYTHSRLSKQDTHTQQEQSRGPREETEVPCPLAPGEQVYLRVLRMKWNEPRREGPYKVVRATPKAVQVEGSTSWYHLNHCTRVPKIKPRRLEVQVVEEEGGQAADTSEESQEDTREGLKIRVKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F488</accession>
    <name>A0A979F488_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Endoplasmic reticulum membrane protein complex subunit 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ER membrane protein complex subunit 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC108273281</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047014921.1">
        <property type="nucleotide sequence ID" value="XM_047158965.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979F488"/>
    <dbReference type="GeneID" id="108273281"/>
    <dbReference type="KEGG" id="ipu:108273281"/>
    <dbReference type="OrthoDB" id="27095at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019008">
        <property type="entry name" value="Beta_sandwich_EMC7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039163">
        <property type="entry name" value="EMC7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13605">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13605:SF4">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09430">
        <property type="entry name" value="EMC7_beta-sandw"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036963550" description="Endoplasmic reticulum membrane protein complex subunit 7" evidence="10">
        <location>
            <begin position="24"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="ER membrane protein complex subunit 7 beta-sandwich" evidence="11">
        <location>
            <begin position="51"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF09430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_047014921.1"/>
        </source>
    </evidence>
    <sequence length="207" mass="23178" checksum="9A506DC2281270D3" modified="2023-02-22" version="1">MQHLKRILDVYIVLQSAFVLSCCFTESEPGPGAVNGDRLKIEGRVVVSGVKAQDWVSSARVLVEGGEYIGFLRTNESFAVHDVPSGSYVVEIISPSYKLKPARVDITSKGKMRARLVNYIKTSVVSKVIRQPYPLHMRASGPHSYFMKRETWEWTDLLMNPMVMMMVLPLLIIVLEREILQGQEPLGYPGVGVLGLGHPLEWDVVSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QQ41</accession>
    <name>A0A9F7QQ41_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13 14">C-X-C motif chemokine 11-6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC108261914</name>
        <name evidence="12" type="synonym">LOC128628939</name>
        <name evidence="13" type="synonym">LOC128628945</name>
    </gene>
    <organism evidence="11 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12 13 14" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12 13 14">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Ligand for cxcr3.2. Chemotactic for macrophages.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the intercrine alpha (chemokine CxC) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053530423.1">
        <property type="nucleotide sequence ID" value="XM_053674448.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053530511.1">
        <property type="nucleotide sequence ID" value="XM_053674536.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053530600.1">
        <property type="nucleotide sequence ID" value="XM_053674625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QQ41"/>
    <dbReference type="GeneID" id="108261914"/>
    <dbReference type="KEGG" id="ipu:108261914"/>
    <dbReference type="KEGG" id="ipu:128628939"/>
    <dbReference type="KEGG" id="ipu:128628945"/>
    <dbReference type="GeneTree" id="ENSGT01030000234997"/>
    <dbReference type="OMA" id="SAYCENV"/>
    <dbReference type="OrthoDB" id="8872899at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042056">
        <property type="term" value="F:chemoattractant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00273">
        <property type="entry name" value="Chemokine_CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000004">
        <property type="entry name" value="C-X-C motif chemokine"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001089">
        <property type="entry name" value="Chemokine_CXC"/>
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    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033899">
        <property type="entry name" value="CXC_Chemokine_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015:SF191">
        <property type="entry name" value="C-X-C MOTIF CHEMOKINE 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015">
        <property type="entry name" value="SMALL INDUCIBLE CYTOKINE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00436">
        <property type="entry name" value="INTERLEUKIN8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00437">
        <property type="entry name" value="SMALLCYTKCXC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00199">
        <property type="entry name" value="SCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="4" id="KW-0202">Cytokine</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044698391" evidence="9">
        <location>
            <begin position="21"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="10">
        <location>
            <begin position="26"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053530423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_053530511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_053530600.1"/>
        </source>
    </evidence>
    <sequence length="95" mass="10268" checksum="ECCFF42D342A66E8" modified="2023-06-28" version="1">MKSAAVFVVFACLLIVHVQGQARTSVTRCLCQGPAANVVRPQRIDKIEIHPASATCENVEIIVTLKNGAGKKCLNPESEFTKKYITAGLEKRSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QX82</accession>
    <name>A0A9F7QX82_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">26S proteasome regulatory subunit 6B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">26S proteasome AAA-ATPase subunit RPT3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Proteasome 26S subunit ATPase 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">psmc4</name>
        <name evidence="18" type="synonym">prs6b</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC4 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases including PSMC4 and few additional components. Interacts with NR1I3. Interacts with PAAF1. Interacts with TRIM5. Interacts with ZFAND1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053530661.1">
        <property type="nucleotide sequence ID" value="XM_053674686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QX82"/>
    <dbReference type="GeneID" id="100528979"/>
    <dbReference type="CTD" id="5704"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000018">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000046">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000033">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9 18" id="KW-0647">Proteasome</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="181"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="20"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_053530661.1"/>
        </source>
    </evidence>
    <sequence length="401" mass="45529" checksum="4757FA18DC9055E6" modified="2023-06-28" version="1">MLSSRPQTGLSFLAPEPEDLEDLYSRYKKLQQELEFLEVQEEYIKDEQKNLKKEFLHAQEEVKRIQSIPLVIGQFLEAVDQNTAIVGSTTGSNYYVRILSTIDRELLKPNASVALHKHSNALVDVLPPEADSSIMMLTSDQKPDVMYADIGGMDIQKQEVREAVELPLTHFELYKQIGIDPPRGVLMYGPPGCGKTMLAKAVAHHTTAAFIRVVGSEFVQKYLGEGPRMVRDVFRLAKENAPAIIFIDEIDAIATKRFDAQTGADREVQRILLELLNQMDGFDQNVNVKVIMATNRADTLDPALLRPGRLDRKIEFPVPDRRQKRLIFTTITSKMNLSEEVDLEDYVARPDKISGADINSICQEAGMLAVRENRYIVLAKDFEKAYKTVIKKDEQEHEFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QXX4</accession>
    <name>A0A9F7QXX4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Metalloendopeptidase</fullName>
            <ecNumber evidence="10">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC108259574</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9 10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9 10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="10"/>
    <dbReference type="RefSeq" id="XP_053532434.1">
        <property type="nucleotide sequence ID" value="XM_053676459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QXX4"/>
    <dbReference type="GeneID" id="108259574"/>
    <dbReference type="KEGG" id="ipu:108259574"/>
    <dbReference type="OrthoDB" id="291007at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000040">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF779">
        <property type="entry name" value="METALLOENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 9 10" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="5 9 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039963732" description="Metalloendopeptidase" evidence="10">
        <location>
            <begin position="18"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12A" evidence="11">
        <location>
            <begin position="49"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_053532434.1"/>
        </source>
    </evidence>
    <sequence length="250" mass="28478" checksum="8CD8014E3BC93DB2" modified="2023-06-28" version="1">MIHYLTYFNSLLVLSVGELLERANRDIVRGADEPTVVGDIAVEDDNGRNADPCVSTGCKWPKSSDGLVYVPYIIANHFSSRELQVIQRGLDSFASVSCIRFVPRYNERDYLSIESRSGCYSYVGRLGYGQTVSLDRNGCIYHNTVQHELLHALGFNHEQTRSDRDNHIRILWENIIENMKYNFDKIATLNQGTPYDYDSVMQYHKTGFSKNGKPTMEPIPNSNVEIGLAREMSQNDIVRINRVYECSYGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R999</accession>
    <name>A0A9F7R999_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Caspase-6</fullName>
            <ecNumber evidence="14">3.4.22.59</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC108262968</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Strict requirement for Asp at position P1 and has a preferred cleavage sequence of Val-Glu-His-Asp-|-.</text>
            <dbReference type="EC" id="3.4.22.59"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (Caspase-6 subunit p18) and a 11 kDa (Caspase-6 subunit p11) subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the peptidase C14A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.59" evidence="14"/>
    <dbReference type="RefSeq" id="XP_053535837.1">
        <property type="nucleotide sequence ID" value="XM_053679862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R999"/>
    <dbReference type="GeneID" id="108262968"/>
    <dbReference type="KEGG" id="ipu:108262968"/>
    <dbReference type="OrthoDB" id="6116485at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043525">
        <property type="term" value="P:positive regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00032">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1460:FF:000001">
        <property type="entry name" value="Caspase-3 preproprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016129">
        <property type="entry name" value="Caspase_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002398">
        <property type="entry name" value="Pept_C14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002138">
        <property type="entry name" value="Pept_C14_p10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015917">
        <property type="entry name" value="Pept_C14A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10454">
        <property type="entry name" value="CASPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10454:SF206">
        <property type="entry name" value="CASPASE-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00376">
        <property type="entry name" value="IL1BCENZYME"/>
    </dbReference>
    <dbReference type="SMART" id="SM00115">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01121">
        <property type="entry name" value="CASPASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50207">
        <property type="entry name" value="CASPASE_P10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="9" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Caspase family p20" evidence="18">
        <location>
            <begin position="24"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p10" evidence="17">
        <location>
            <begin position="172"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_053535837.1"/>
        </source>
    </evidence>
    <sequence length="266" mass="30276" checksum="42E32424A8428187" modified="2023-06-28" version="1">MSGTGAQSKSKTDSPMEYKMDHKRRGMALIFNHENFKNEKKLTKRCGTNIDRDNLKERFQGLGFEVNCHNDKSKAEVLKEIRKAAAANHKDADCFVCIFLTHGEEGKINAYDDKIKIKEITDYFRGDQCKSLVGKPKIFIFQACAGKKREVAVTGMMAGDSDDEEQVEEAANIYTIPAGADFLMCYSVAEGFVSFRLIESGTFYIQDLCETVQQYGSTMEFTELLTLVNLKVSRRTADGQDFRNKKQMPCFTSMLTKRLYFRSKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RAI2</accession>
    <name>A0A9F7RAI2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phytanoyl-CoA 2-hydroxylase interacting protein-like a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">phyhipla</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May play a role in the development of the central system.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PHYHIP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053538581.1">
        <property type="nucleotide sequence ID" value="XM_053682606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RAI2"/>
    <dbReference type="GeneID" id="108270363"/>
    <dbReference type="CTD" id="790943"/>
    <dbReference type="OrthoDB" id="6101761at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000277">
        <property type="entry name" value="Phytanoyl-CoA hydroxylase-interacting protein-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042868">
        <property type="entry name" value="PHYHIP/PHYHIPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045545">
        <property type="entry name" value="PHYIP/PHIPL_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15698:SF8">
        <property type="entry name" value="PHYTANOYL-COA HYDROXYLASE-INTERACTING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15698">
        <property type="entry name" value="PROTEIN CBG15099"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19281">
        <property type="entry name" value="PHYHIP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="44"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00037571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053538581.1"/>
        </source>
    </evidence>
    <sequence length="369" mass="41558" checksum="8B87E537E353005C" modified="2023-06-28" version="1">MAVPGQLSPREGTVAKNLSLESLQLCERDGKVEESGVVEMELPVPMNIRISNITCDSFKICWDMDPRTKEKITHYFIDLNKKENKNSNKFKHKDVPTKLVAKAVPLPMTVRGHWFLSPRTEYTVAVQTASKQGDGDYAVSEWSDIVEFCTADYSPVHLTQLLQKAEVIAGRMLPFSVFYRNQNQEYFDYCSNAQGGRMLPSVKDNSGSHGSPLSGKLEGIFFSCNTEFNTGEPPQDSPYGRYRFQISAEELFGPDTRLYFGDFYCMYTAYHYVILVLAPRGSPGDTYCSERLPELDATNNRFLTRVCAEDGRVEFRHAQDVILEIIYTEPVELTRGTVAQISGHQLMSLSTINAKKDPSCKTCNISVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RAJ0</accession>
    <name>A0A9F7RAJ0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">PR domain zinc finger protein 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">PR domain-containing protein 12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC108259800</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Transcriptional regulator necessary for the development of nociceptive neurons, playing a key role in determining the nociceptive lineage from neural crest cell progenitors. Initiates neurogenesis and activates downstream pro-neuronal transcription factors, such as NEUROD1, BRN3A, and ISL1, specifically within nociceptive neurons, while repressing non-nociceptor cell fates. Essential for the proper function of nociceptors in adults, influencing both their excitability and their gene expression, thereby impacting how these neurons respond to various pain stimuli.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with EHMT2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053532978.1">
        <property type="nucleotide sequence ID" value="XM_053677003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RAJ0"/>
    <dbReference type="GeneID" id="108259800"/>
    <dbReference type="KEGG" id="ipu:108259800"/>
    <dbReference type="OrthoDB" id="8117402at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140713">
        <property type="term" value="F:histone chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990226">
        <property type="term" value="F:histone methyltransferase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022008">
        <property type="term" value="P:neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19196">
        <property type="entry name" value="PR-SET_PRDM12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000018">
        <property type="entry name" value="PR domain zinc finger protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000501">
        <property type="entry name" value="PR domain zinc finger protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000526">
        <property type="entry name" value="PR domain zinc finger protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001416">
        <property type="entry name" value="PR domain zinc finger protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044406">
        <property type="entry name" value="PRDM12_PR/SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF20">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21549">
        <property type="entry name" value="PRDM2_PR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="19">
        <location>
            <begin position="111"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="297"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="325"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="353"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="47"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="371"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00079747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00093312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00093576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_053532978.1"/>
        </source>
    </evidence>
    <sequence length="415" mass="46904" checksum="B0F4F86F0ADD2391" modified="2023-06-28" version="1">MGSVFPADALRMKSGFKSPNLSLCEIITSDVLHSFLYGRWRNVLGEQPHHHHHPHPLHPHPHHHHPHPHHLHQVSEDKPQHPFSKTAFTAEVLAQSFSGEVQKLSSLVLPSEVIIAQSSIPGEGLGIFSKTWIKAGTEMGPFTGRVLYPQHVDLLKNNNLMWEVFNEDGTVRCFIDASQEDQRSWMTYIKCARNEQEQNLEVVQIGSSIFYKAVETIPPDQELLVWYGNSHNSFLGIPGVPGIEEEHQKRTRNGKYDKRDFKNLTLSSVFFSDDSHSSDSNPSTSPPSMSSLASSRMRCVICHRGFNSRSNLRSHMRIHTLDKPFACRFCNRRFSQSSTLRNHVRLHTGERPYKCHVCQSAYSQLAGLRAHQKSARHRPAGNNTETRRPSPASPSQVASMAQTVPLVHHIATMVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RD39</accession>
    <name>A0A9F7RD39_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16 17 18">Zinc finger protein 135</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16 17 18" type="primary">LOC128628793</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16 17 18" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16 17 18">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053539961.1">
        <property type="nucleotide sequence ID" value="XM_053683986.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053539962.1">
        <property type="nucleotide sequence ID" value="XM_053683987.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053539963.1">
        <property type="nucleotide sequence ID" value="XM_053683988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RD39"/>
    <dbReference type="GeneID" id="128628793"/>
    <dbReference type="KEGG" id="ipu:128628793"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:004935">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000966">
        <property type="entry name" value="ZFP90 zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000512">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000184">
        <property type="entry name" value="Zinc finger protein 333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002402">
        <property type="entry name" value="Zinc finger protein 347"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001270">
        <property type="entry name" value="zinc finger protein 583 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF31">
        <property type="entry name" value="LOW QUALITY PROTEIN: ZINC FINGER PROTEIN 721-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="137"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_053539961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_053539962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_053539963.1"/>
        </source>
    </evidence>
    <sequence length="223" mass="25501" checksum="2F43EE2B0BFA907F" modified="2023-06-28" version="1">MKSESRRRSLGKSPQTPAATGSKMYHCSDCGKSFSKSHHLKDHKRIHTGEKPYDCSQCGKSFARQSTLQQHQRIHTGEKPYHCSQCGKSFTCRSHLKQHQRIHTGEKPYHCSYCGKSFTCRSHLQQHEHIHTGQKPYHCSQCGKSFTCQSSLQRHERIHTGEKPYYCLQCGKGFRDQSALYSHQQCHTGQKPYLCGQCGRSYTHSGSLRTHKCSKIKPSALDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RFM0</accession>
    <name>A0A9F7RFM0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">NACHT, LRR and PYD domains-containing protein 12-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC128634613</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053541296.1">
        <property type="nucleotide sequence ID" value="XM_053685321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RFM0"/>
    <dbReference type="GeneID" id="128634613"/>
    <dbReference type="KEGG" id="ipu:128634613"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000782">
        <property type="entry name" value="Si:ch211-196h16.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000210">
        <property type="entry name" value="Si:dkey-16p6.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001632">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="7">
        <location>
            <begin position="99"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_053541296.1"/>
        </source>
    </evidence>
    <sequence length="1016" mass="114927" checksum="F7804A00B2A85765" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RP90</accession>
    <name>A0A9F7RP90_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">deoxyribonuclease II</fullName>
            <ecNumber evidence="3">3.1.22.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">dnase2b</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.</text>
            <dbReference type="EC" id="3.1.22.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNase II family.</text>
    </comment>
    <dbReference type="EC" id="3.1.22.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_053539228.1">
        <property type="nucleotide sequence ID" value="XM_053683253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RP90"/>
    <dbReference type="GeneID" id="108270716"/>
    <dbReference type="CTD" id="58511"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004531">
        <property type="term" value="F:deoxyribonuclease II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006309">
        <property type="term" value="P:apoptotic DNA fragmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004947">
        <property type="entry name" value="DNase_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10858">
        <property type="entry name" value="DEOXYRIBONUCLEASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10858:SF2">
        <property type="entry name" value="DEOXYRIBONUCLEASE-2-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03265">
        <property type="entry name" value="DNase_II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039910507" description="deoxyribonuclease II" evidence="5">
        <location>
            <begin position="25"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053539228.1"/>
        </source>
    </evidence>
    <sequence length="297" mass="34404" checksum="1CF58D5690B8B391" modified="2023-06-28" version="1">MFLFRPFRLIFFQVSFFLSSLCEGEISCLNEDGQPVDWFIMYKLPKYKQGNVGSGVDYMYLDPSLPNWQMSKYSVNTSEGALGRTLSQLYQRYMSNSSAYMLYNDAPPLLKYEYNYGHTKAQQLAYINPYTYNCSMPSAYYTEMAEMAQICAGKTVTVVPRRRLEKLMSVKGETFLSFAKSHSYVDDIYAGWIAQTLNTDFLVETWQREAHQLPSNCSLPYHVMNIKRVSLSELVTFSSYDDHSKWCVSWEHQTQWTCLGDLNRESRQAWRGGGLICTPNSAMYKAFRSAVAWYKNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RRN7</accession>
    <name>A0A9F7RRN7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transmembrane protein 238</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108273541</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053541257.1">
        <property type="nucleotide sequence ID" value="XM_053685282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RRN7"/>
    <dbReference type="GeneID" id="108273541"/>
    <dbReference type="KEGG" id="ipu:108273541"/>
    <dbReference type="OrthoDB" id="9047238at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029365">
        <property type="entry name" value="TMEM238"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28613">
        <property type="entry name" value="SI:CH211-232M10.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28613:SF7">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 238"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15125">
        <property type="entry name" value="TMEM238"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053541257.1"/>
        </source>
    </evidence>
    <sequence length="175" mass="19296" checksum="4C14222E972F3D0E" modified="2023-06-28" version="1">MVPRCIGGCVPLFVLGVVFDVVGLIVLLVGALANLRLDGRFYGDFLVYTGSIVVFLSLMWWVLWYTGNATIPSDELEKTPLDNLALWARRLSTSIAHTGAKSMEVGEKNKSVDAQELMNGSMPVYINIPTRTAWQISGMEGYDNAAFERSVDMPPTSQKTVQLDILGNGRIERLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RTX5</accession>
    <name>A0A9F7RTX5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cystine/glutamate transporter-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC128635371</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053544031.1">
        <property type="nucleotide sequence ID" value="XM_053688056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RTX5"/>
    <dbReference type="GeneID" id="128635371"/>
    <dbReference type="KEGG" id="ipu:128635371"/>
    <dbReference type="OrthoDB" id="10062876at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF246">
        <property type="entry name" value="CYSTINE_GLUTAMATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053544031.1"/>
        </source>
    </evidence>
    <sequence length="124" mass="14510" checksum="C0EE8BDE1FB6FF0F" modified="2023-06-28" version="1">MLARGDIYQLINFASFSCWFFIALVTLGLLIHRYRFPDHPRTFKVPLAVPVIFTVVCFFIVGLSLYSDPWNTGCSFAVTFTGIPVYYLTIKHSYIPNRWRKAINYYTNQLQILLEVVQQEVQTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TD85</accession>
    <name>A0A9F7TD85_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Vesicle-trafficking protein SEC22c isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">sec22c</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in vesicle transport between the ER and the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053530703.1">
        <property type="nucleotide sequence ID" value="XM_053674728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TD85"/>
    <dbReference type="GeneID" id="108256373"/>
    <dbReference type="CTD" id="9117"/>
    <dbReference type="OMA" id="QDRTNDC"/>
    <dbReference type="OrthoDB" id="1719357at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059071">
        <property type="entry name" value="SEC22a-c_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043546">
        <property type="entry name" value="Sec22a/c"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46258">
        <property type="entry name" value="LONGIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46258:SF2">
        <property type="entry name" value="VESICLE-TRAFFICKING PROTEIN SEC22C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25970">
        <property type="entry name" value="SEC22a_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039928835" evidence="12">
        <location>
            <begin position="16"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="250"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Longin" evidence="13">
        <location>
            <begin position="8"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_053530703.1"/>
        </source>
    </evidence>
    <sequence length="348" mass="38903" checksum="77D64E8D66532661" modified="2023-06-28" version="1">MSLIVFVLVARLRDGLPLSASTDFQHSRELQERKQQIRTVSKSLNSLPERGIIMGHELHIYFFMSDGVAYLSVCMCSVSAAVAFSFLEDLRWEFMASFDSSAVARACRPYPFLDFDSVIQKLQQHYNQSGGPALGVTLSTVEEELSLSPPQVLTMDEIQLSNGVANGHAEQTASSGQTQRLEPVSAPGILSLILNIMCAALNLIRGVHLIEYTFQDDYDGLWNVVAFFVAFVCCVCQCQLYLFHSSRKKIKSLTLLTAIVLCNGFLFGLRNVWQLTFHVFVAFLSTLLILNRKLLDRSSDCGVISQQEDQRAVYGRKASQFEAEKRGKINQRHSQTLGIASTTIWNVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TEM6</accession>
    <name>A0A9F7TEM6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Opioid growth factor receptor isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC128628671</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the opioid growth factor receptor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053530041.1">
        <property type="nucleotide sequence ID" value="XM_053674066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TEM6"/>
    <dbReference type="GeneID" id="128628671"/>
    <dbReference type="KEGG" id="ipu:128628671"/>
    <dbReference type="OrthoDB" id="9030204at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140625">
        <property type="term" value="F:opioid growth factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006757">
        <property type="entry name" value="OGF_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039574">
        <property type="entry name" value="OGFr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14015:SF1">
        <property type="entry name" value="OPIOID GROWTH FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14015">
        <property type="entry name" value="OPIOID GROWTH FACTOR RECEPTOR OGFR ZETA-TYPE OPIOID RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04664">
        <property type="entry name" value="OGFr_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Opioid growth factor receptor (OGFr) conserved" evidence="3">
        <location>
            <begin position="82"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="296"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="368"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="391"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053530041.1"/>
        </source>
    </evidence>
    <sequence length="432" mass="50854" checksum="651FB5DFE64733EF" modified="2023-06-28" version="1">MDRSPRSLRQDRVMGNTPNFSTDYDEQYDSTWEDESDGEETRESSMQWSHRNTHAAKDMQTFRHKFRKTQHDDSTDDYLEDDEELPNLRFYQNDMPFYPDGVLIKDFHIFWFGDYDRLEYVHSYIQWLFPIQEKGMNYASRELSLKEIKLFRTDEEVQRRLLTSYKLMLDFYGIELVSEDSGEVKRAKNWKEQFANLNRNTHNNLRIIRILKCLGLLGFRHYQKPLVHFFLEETLVNNQLPRVKYSVLDYFMFAVLDKDERKDLIKYAYCHFKPKEDFVWCPRRIRNKLEKETVSHKLNAGSNEGESSDSTSQSEQNDLMNSRVHKKNEFKTDTNEDTGNNKSLRCESRNDENVSGESQDSEEQASQHTERDSKENDSKEIQKSDSESSEDSMATGTATNTEANSTHYDAEKSSDQNSSKYTFSPGEANASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TFD9</accession>
    <name>A0A9F7TFD9_ICTPU</name>
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        <submittedName>
            <fullName evidence="15">NACHT, LRR and PYD domains-containing protein 12 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC108256308</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053530836.1">
        <property type="nucleotide sequence ID" value="XM_053674861.1"/>
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    <dbReference type="KEGG" id="ipu:108256308"/>
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        <property type="component" value="Chromosome 23"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Bbox2_TRIM16-like"/>
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        <property type="entry name" value="LRR_RI"/>
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        <property type="entry name" value="Si:dkey-16p6.1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
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    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
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    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
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    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
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    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
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    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
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    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
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    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="13"/>
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    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="11">
        <location>
            <begin position="194"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="13">
        <location>
            <begin position="406"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="12">
        <location>
            <begin position="1313"/>
            <end position="1505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="52"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_053530836.1"/>
        </source>
    </evidence>
    <sequence length="1505" mass="169456" checksum="7DE84E1C50F6A02D" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TGJ8</accession>
    <name>A0A9F7TGJ8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Histone H2B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC128629970</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053534498.1">
        <property type="nucleotide sequence ID" value="XM_053678523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TGJ8"/>
    <dbReference type="GeneID" id="128629970"/>
    <dbReference type="KEGG" id="ipu:128629970"/>
    <dbReference type="OrthoDB" id="8903231at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5 6" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_053534498.1"/>
        </source>
    </evidence>
    <sequence length="100" mass="11004" checksum="B90594781688AA5C" modified="2023-06-28" version="1">MPDPAKTAPKKGSKKAVTKTAGKGGKKRRKSRKESYAIYVYKVLKQRIAGESSRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TH98</accession>
    <name>A0A9F7TH98_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">zgc:55461</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053533162.1">
        <property type="nucleotide sequence ID" value="XM_053677187.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TH98"/>
    <dbReference type="GeneID" id="108260450"/>
    <dbReference type="KEGG" id="ipu:108260450"/>
    <dbReference type="OrthoDB" id="1662883at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000006">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000003">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="14">
        <location>
            <begin position="63"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="15">
        <location>
            <begin position="262"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="442"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="445"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_053533162.1"/>
        </source>
    </evidence>
    <sequence length="461" mass="51721" checksum="40F2FAE859D8863E" modified="2023-06-28" version="1">MSFPQGRECVWETLLKMREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEGEFEEEAEEELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TRP1</accession>
    <name>A0A9F7TRP1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">VPS37B subunit of ESCRT-I b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">vps37bb</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in cell growth and differentiation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS37 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053542926.1">
        <property type="nucleotide sequence ID" value="XM_053686951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TRP1"/>
    <dbReference type="GeneID" id="128628634"/>
    <dbReference type="KEGG" id="ipu:128628634"/>
    <dbReference type="CTD" id="566797"/>
    <dbReference type="OrthoDB" id="10004364at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036258">
        <property type="term" value="P:multivesicular body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043162">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039702">
        <property type="term" value="P:viral budding via host ESCRT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.660:FF:000003">
        <property type="entry name" value="vacuolar protein sorting-associated protein 37B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037202">
        <property type="entry name" value="ESCRT_assembly_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009851">
        <property type="entry name" value="Mod_r"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13678">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13678:SF9">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 37B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07200">
        <property type="entry name" value="Mod_r"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140111">
        <property type="entry name" value="Endosomal sorting complex assembly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51314">
        <property type="entry name" value="VPS37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VPS37 C-terminal" evidence="9">
        <location>
            <begin position="50"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053542926.1"/>
        </source>
    </evidence>
    <sequence length="222" mass="24350" checksum="A78E012A9A8B4CAC" modified="2023-06-28" version="1">MRPTSSTNALLGCSCSRTYPGNTGYEEGIHPEWNATPPLCTMCTHNPPSGRFLGDHNSGKSSLDTLLALLQAEGAKIEEETENMADSFLTGDLSLDNFIETYQSKRKLAHLRRVKIDKLQEMVLKCFQMPQASVEHVQSQTPTSTPPFDGQVNGFPVPMRAAPSTSIQLTGQQPALPYPATPYPPVQMPNMVPSYTGPLTQQYTPAFPQRPGLPPRTGFIMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2007-09-11" modified="2026-01-28" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>A7LFX3</accession>
    <name>A7LFX3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Growth hormone</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="13">Somatotropin precursor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">gh1</name>
        <name evidence="13" type="synonym">gh</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="1992" name="Mol. Mar. Biol. Biotechnol." volume="1" first="239" last="249">
            <title>Chemical identification of catfish growth hormone and prolactin.</title>
            <authorList>
                <person name="Watanabe K."/>
                <person name="Igarashi A."/>
                <person name="Noso T."/>
                <person name="Chen T.T."/>
                <person name="Durham R.A."/>
                <person name="Kawauchi H."/>
            </authorList>
            <dbReference type="PubMed" id="1308206"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="1993" name="Mol. Mar. Biol. Biotechnol." volume="2" first="198" last="206">
            <title>Structure of the channel catfish (Ictalurus punctatus) growth hormone gene and its evolutionary implications.</title>
            <authorList>
                <person name="Tang Y."/>
                <person name="Lin C.M."/>
                <person name="Chen T.T."/>
                <person name="Kawauchi H."/>
                <person name="Dunham R.A."/>
                <person name="Powers D.A."/>
            </authorList>
            <dbReference type="PubMed" id="8293072"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2007-07" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Lin T."/>
                <person name="Yan Q.-G."/>
                <person name="Fing T."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="13" key="4">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="12" key="5">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="6">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the somatotropin/prolactin family.</text>
    </comment>
    <dbReference type="EMBL" id="EU009499">
        <property type="protein sequence ID" value="ABS70461.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187174.1">
        <property type="nucleotide sequence ID" value="NM_001200245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A7LFX3"/>
    <dbReference type="GeneID" id="100305005"/>
    <dbReference type="KEGG" id="ipu:100305005"/>
    <dbReference type="CTD" id="2688"/>
    <dbReference type="OrthoDB" id="9925773at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070186">
        <property type="term" value="F:growth hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005131">
        <property type="term" value="F:growth hormone receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060396">
        <property type="term" value="P:growth hormone receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042538">
        <property type="term" value="P:hyperosmotic salinity response"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045927">
        <property type="term" value="P:positive regulation of growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046427">
        <property type="term" value="P:positive regulation of receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903576">
        <property type="term" value="P:response to L-arginine"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043434">
        <property type="term" value="P:response to peptide hormone"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="CDD" id="cd10285">
        <property type="entry name" value="somatotropin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.10:FF:000009">
        <property type="entry name" value="Growth hormone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034975">
        <property type="entry name" value="Somatotropin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001400">
        <property type="entry name" value="Somatotropin/Prolactin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018116">
        <property type="entry name" value="Somatotropin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11417:SF2">
        <property type="entry name" value="SOMATOTROPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11417">
        <property type="entry name" value="SOMATOTROPIN,PROLACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00103">
        <property type="entry name" value="Hormone_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00836">
        <property type="entry name" value="SOMATOTROPIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00266">
        <property type="entry name" value="SOMATOTROPIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00338">
        <property type="entry name" value="SOMATOTROPIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 9" id="KW-0372">Hormone</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10 13" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035982252" evidence="10 13">
        <location>
            <begin position="21"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABS70461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="NP_001187174.1"/>
        </source>
    </evidence>
    <sequence length="200" mass="22441" checksum="9098605F29DC7D73" modified="2007-09-11" version="1">MARALVLLSVVVASLLFSQGATFESQRLFNNAVIRVQHLHQLAAKMMDDFEEALLPEERKQLSKIFPLSFCNSDSIEAPAGKDEAQKSSVLKLLHTSYRLIESWEFPSRNLGNPNHISEKLADLKMGIGVLIEGCVDGQTGLDENDSLAPPFEDFYQTLSEGNLRKSFRLLSCFKKDMHKVETYLSVAKCRRSLDSNCTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3TDX7</accession>
    <name>E3TDX7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ER membrane protein complex subunit 9</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="4">Upf0172 protein fam158a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">F158A</name>
        <name evidence="6" type="synonym">emc9</name>
        <name evidence="6" type="synonym">f158a</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Component of the ER membrane protein complex (EMC). EMC8 and EMC9 are mutually exclusive subunits of the EMC complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the EMC8/EMC9 family.</text>
    </comment>
    <dbReference type="EMBL" id="GU588557">
        <property type="protein sequence ID" value="ADO28513.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001188072.1">
        <property type="nucleotide sequence ID" value="NM_001201143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TDX7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000031291"/>
    <dbReference type="GeneID" id="100528873"/>
    <dbReference type="KEGG" id="ipu:100528873"/>
    <dbReference type="CTD" id="51016"/>
    <dbReference type="GeneTree" id="ENSGT00390000006738"/>
    <dbReference type="OMA" id="CLSDCVP"/>
    <dbReference type="OrthoDB" id="194468at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08060">
        <property type="entry name" value="MPN_UPF0172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005366">
        <property type="entry name" value="EMC8/9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12941">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12941:SF12">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03665">
        <property type="entry name" value="UPF0172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPN" evidence="3">
        <location>
            <begin position="4"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00046436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADO28513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="NP_001188072.1"/>
        </source>
    </evidence>
    <sequence length="212" mass="23503" checksum="BE23329B082BD932" modified="2011-01-11" version="1">MSEVELSCMACVKMFLHASVYPRCSVNGLLLAPAGGGRGGGVCEEGVCVTDCVPLLHSHLPLAMIAQLALTQVDVWCSQTQQRIVGYYQANATLSDSSPTACAFKMADKILEQSSNAVLLMIDGKKMSPGYRVPPIVMYEHKDSRWTLKDKRTIMLRQWEETRAITAQILNSRDHMLLVDFDSHLDDITKDWTNQKLNAKITELICPANGNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3TF55</accession>
    <name>E3TF55_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Sodium/potassium-transporting ATPase subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">AT233</name>
        <name evidence="22" type="synonym">at233</name>
        <name evidence="22" type="synonym">atp1b1</name>
        <name evidence="22" type="synonym">atp1b1a</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 22" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the X(+)/potassium ATPases subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="GU588989">
        <property type="protein sequence ID" value="ADO28941.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001188080.1">
        <property type="nucleotide sequence ID" value="NM_001201151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TF55"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013986"/>
    <dbReference type="GeneID" id="100528881"/>
    <dbReference type="KEGG" id="ipu:100528881"/>
    <dbReference type="CTD" id="64267"/>
    <dbReference type="OrthoDB" id="5912413at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005890">
        <property type="term" value="C:sodium:potassium-exchanging ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000062">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1660">
        <property type="entry name" value="Na, k-atpase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000402">
        <property type="entry name" value="Na/K_ATPase_sub_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038702">
        <property type="entry name" value="Na/K_ATPase_sub_beta_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01107">
        <property type="entry name" value="Na_K_ATPase_bet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11523">
        <property type="entry name" value="SODIUM/POTASSIUM-DEPENDENT ATPASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11523:SF10">
        <property type="entry name" value="SODIUM_POTASSIUM-TRANSPORTING ATPASE SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00287">
        <property type="entry name" value="Na_K-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00390">
        <property type="entry name" value="ATPASE_NA_K_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00391">
        <property type="entry name" value="ATPASE_NA_K_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="20" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 19" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="11" id="KW-0915">Sodium</keyword>
    <keyword evidence="16" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6" id="KW-0740">Sodium/potassium transport</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 19" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="35"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00025540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00038795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ADO28941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="NP_001188080.1"/>
        </source>
    </evidence>
    <sequence length="301" mass="34456" checksum="63D300C119783C70" modified="2011-01-11" version="1">MPANKEGDGGWKAFLWNSEKKEFLGRTGGSWFKIFIFYVIFYGCLAGIFIGTIQAMLMTLSNYKPTYQDRVAPPGLSHTPRSDKSEISYILGEEDSYRDYTKAMKELLEPYKDEKQVDDSKYDDCGNAPGKYIERGDLESDTGVRKACRFKRSWLGDCSGLEDTSFGFKAGKPCLIVKLNRIVNFRPRPPKTNTSLPVVAGGKIPQFLIPVYCSSKKEEDADKLREVKYFGLGQGYPLQYYPYYGKLLHPHYLQPLVGIQFTNISINQELRVECKVYGENIDYSDKDRYQGRFDLKIMIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3TF66</accession>
    <name>E3TF66_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transmembrane protein 170A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">T170A</name>
        <name evidence="14" type="synonym">t170a</name>
        <name evidence="14" type="synonym">tmem170a</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TMEM170 family.</text>
    </comment>
    <dbReference type="EMBL" id="GU589000">
        <property type="protein sequence ID" value="ADO28952.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187661.1">
        <property type="nucleotide sequence ID" value="NM_001200732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TF66"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005889"/>
    <dbReference type="GeneID" id="100528462"/>
    <dbReference type="KEGG" id="ipu:100528462"/>
    <dbReference type="CTD" id="124491"/>
    <dbReference type="GeneTree" id="ENSGT00940000159189"/>
    <dbReference type="OMA" id="GRFMSVG"/>
    <dbReference type="OrthoDB" id="13807at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071786">
        <property type="term" value="P:endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019334">
        <property type="entry name" value="TMEM170A/B/YPR153W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22779">
        <property type="entry name" value="SD17342P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22779:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 170A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10190">
        <property type="entry name" value="Tmemb_170"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADO28952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="NP_001187661.1"/>
        </source>
    </evidence>
    <sequence length="140" mass="15173" checksum="B25FECE710D120A5" modified="2011-01-11" version="1">MQDDYNLPEIGFVQQILSLNLVPRKNATCGRNDTSLCDFSEMWYGVFLWAGVSSLVFHLPAALLALATLRGHKMARFFPLAIMLMAIIGPLLGGVLTSAAIAGVYMAAGKRMISLEAFVFGVGQSFCVLVISFLRVLATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3TG16</accession>
    <name>E3TG16_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein LOC100529014</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein c17orf72-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CQ072</name>
        <name evidence="5" type="synonym">cq072</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2016" name="Biol. Reprod." volume="95" first="19" last="19">
            <title>Transcriptome Display During Testicular Differentiation of Channel Catfish (Ictalurus punctatus) as Revealed by RNA-Seq Analysis.</title>
            <authorList>
                <person name="Zeng Q."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Zhang Y."/>
                <person name="Yuan Z."/>
                <person name="Jiang C."/>
                <person name="Chen A."/>
                <person name="Fu Q."/>
                <person name="Su B."/>
                <person name="Dunham R."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="27307075"/>
            <dbReference type="DOI" id="10.1095/biolreprod.116.138818"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="GU589301">
        <property type="protein sequence ID" value="ADO29252.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001188213.1">
        <property type="nucleotide sequence ID" value="NM_001201284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TG16"/>
    <dbReference type="GeneID" id="100529014"/>
    <dbReference type="KEGG" id="ipu:100529014"/>
    <dbReference type="CTD" id="100529014"/>
    <dbReference type="OrthoDB" id="8962708at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027904">
        <property type="entry name" value="DUF4587"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038915">
        <property type="entry name" value="PRR29-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28604">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28604:SF1">
        <property type="entry name" value="PROLINE-RICH PROTEIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15248">
        <property type="entry name" value="DUF4587"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4587" evidence="2">
        <location>
            <begin position="44"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADO29252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="NP_001188213.1"/>
        </source>
    </evidence>
    <sequence length="187" mass="21094" checksum="0484CE42D11B1533" modified="2011-01-11" version="1">MEQSAQIQQDDFSCFQLIQQPAPQPVALFQQFPSAMAPPAPTLRRGHIREDLVELMMMQNAQMHQVIMNNMTMSALSYFGYMQAPEHPGPPVTAEVDPEVYHHHYPCPPCESYPVWVPMSLAPMQSQVPLQTIPSFQDPTYPLHNTHVEHRDRKAVPPPPPPSATGTVGPDVPLAAEHYEVERRQQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5U6A3</accession>
    <name>W5U6A3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9 11">ATP-binding cassette sub-family D member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Abcd4</name>
        <name evidence="11" type="synonym">abcd4</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2013" name="PLoS ONE" volume="8" first="E63895" last="E63895">
            <title>Genome-wide identification, characterization and phylogenetic analysis of 50 catfish ATP-binding cassette (ABC) transporter genes.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Li Q."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23696857"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="JT405742">
        <property type="protein sequence ID" value="AHH37305.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017331077.1">
        <property type="nucleotide sequence ID" value="XM_017475588.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U6A3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000129"/>
    <dbReference type="GeneID" id="108269622"/>
    <dbReference type="KEGG" id="ipu:108269622"/>
    <dbReference type="CTD" id="5826"/>
    <dbReference type="GeneTree" id="ENSGT00950000182955"/>
    <dbReference type="OrthoDB" id="422637at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF59">
        <property type="entry name" value="LYSOSOMAL COBALAMIN TRANSPORTER ABCD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="266"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="44"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="385"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AHH37305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017331077.1"/>
        </source>
    </evidence>
    <sequence length="604" mass="68495" checksum="F03731157E0564DA" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>W5U7G6</accession>
    <name>W5U7G6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6 8 9 10">Nucleosome assembly protein 1-like 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Nap1l4</name>
        <name evidence="8 9 10" type="synonym">nap1l4b</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8 9 10" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8 9 10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the nucleosome assembly protein (NAP) family.</text>
    </comment>
    <dbReference type="EMBL" id="JT407193">
        <property type="protein sequence ID" value="AHH37893.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053532624.1">
        <property type="nucleotide sequence ID" value="XM_053676649.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053532625.1">
        <property type="nucleotide sequence ID" value="XM_053676650.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053532626.1">
        <property type="nucleotide sequence ID" value="XM_053676651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U7G6"/>
    <dbReference type="KEGG" id="ipu:108259663"/>
    <dbReference type="OrthoDB" id="27325at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1500:FF:000001">
        <property type="entry name" value="Nucleosome assembly protein 1-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.90:FF:000001">
        <property type="entry name" value="Nucleosome assembly protein 1-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.90">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037231">
        <property type="entry name" value="NAP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002164">
        <property type="entry name" value="NAP_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00956">
        <property type="entry name" value="NAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143113">
        <property type="entry name" value="NAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="329"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AHH37893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053532624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_053532625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053532626.1"/>
        </source>
    </evidence>
    <sequence length="360" mass="41334" checksum="68AAF602B045A06A" modified="2014-04-16" version="1">MEGDKIEAKGKVGDLVMQNPQALAALQDRLDNLTQGPSVMDSLPLSVRRRVNALKNLQVKSSHIEAKFYEEVHELERKYAALYQPLFDKRKSIVTGTVEPTDEECEWQSESDHEDEELADELQKKASIEEKKAEEGGAAEENPKGIPEFWLTIFRSVDMLSDMLQEADEPILKHLEDIKVTFSGPDEPMSFTLEFHFEPNSYFKNTVLTKVYKMKSEPDTDEPFSFEGPEIIDCEGCEIEWHKGKDVTVKTIKKKQKHKGRGVVRMVTKQVPTDSFFNFFNPIKVSPDSEWDEDSEITLATDFEIGHFFRERIVPRAVLYFTGEALEDEESFEDVELDEGEEEDQEDDMEEEEEEGDAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5U940</accession>
    <name>W5U940_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Rhodopsin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">RH11</name>
        <name evidence="27" type="synonym">LOC108278254</name>
    </gene>
    <organism evidence="25">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="25">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">SDA103</strain>
        </source>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="27">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 22">Membrane</location>
            <topology evidence="1 22">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="21">Contains one covalently linked retinal chromophore.</text>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="JT408360">
        <property type="protein sequence ID" value="AHH38320.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017346967.1">
        <property type="nucleotide sequence ID" value="XM_017491478.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U940"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000023669"/>
    <dbReference type="GeneID" id="108278254"/>
    <dbReference type="KEGG" id="ipu:108278254"/>
    <dbReference type="GeneTree" id="ENSGT01030000234549"/>
    <dbReference type="OMA" id="FTWFMAM"/>
    <dbReference type="OrthoDB" id="5962323at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000018">
        <property type="entry name" value="Rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050125">
        <property type="entry name" value="GPCR_opsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001760">
        <property type="entry name" value="Opsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027430">
        <property type="entry name" value="Retinal_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000732">
        <property type="entry name" value="Rhodopsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019477">
        <property type="entry name" value="Rhodopsin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24240">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10413">
        <property type="entry name" value="Rhodopsin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00238">
        <property type="entry name" value="OPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00579">
        <property type="entry name" value="RHODOPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00238">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 21 22" id="KW-0157">Chromophore</keyword>
    <keyword evidence="11 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 22" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13 19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="2 22" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="12 22" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 21 22" id="KW-0681">Retinal protein</keyword>
    <keyword evidence="4 22" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="14 22" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="15" id="KW-0844">Vision</keyword>
    <keyword evidence="16" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="107"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="257"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="24">
        <location>
            <begin position="57"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="337"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="site" description="Plays an important role in the conformation switch to the active conformation" evidence="17">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(retinylidene)lysine" evidence="21">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="S-palmitoyl cysteine" evidence="20">
        <location>
            <position position="326"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="19">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="113"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600732-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600732-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600732-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR600732-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR600732-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR600732-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU004951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="AHH38320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_017346967.1"/>
        </source>
    </evidence>
    <sequence length="352" mass="39134" checksum="BF0463400595207C" modified="2014-04-16" version="1">MSGLHGFEGENYYIPMSNRTGLVRDPFMYEQYYLAEPWQFRLLAVYMFFLIIFGFPINALTLLVTAQHKRLRQPLNYILVNLALAGLIMVLFGFTITISSAVNGYFYFGYTACAVEGFMATLGGEVALWSLVVLAIERYVVVCKPMGSFKFSATHAIGGIGFTWFMAMTCAGPPLVGWSRYIPEGLQCSCGPDYYTLNPKYNNESYVIYMFVVHFILPVTVIFFTYGRLVCTVKAAAAAQQESASTQKAEKEVTRMVILMVVGFLVAWVPYASVAAWIFFNKGAAFSAQFMAIPAFFSKSSAIFNPIIYVLLNKQFRNCMLTTLFCGKNPLGDDESSSVSTSKTEVSSVSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5U9Y5</accession>
    <name>W5U9Y5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">Ddr2</name>
        <name evidence="26" type="synonym">ddr2l</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="24">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">SDA103</strain>
        </source>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="EMBL" id="JT409595">
        <property type="protein sequence ID" value="AHH38743.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017307075.1">
        <property type="nucleotide sequence ID" value="XM_017451586.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U9Y5"/>
    <dbReference type="GeneID" id="108255554"/>
    <dbReference type="KEGG" id="ipu:108255554"/>
    <dbReference type="CTD" id="565333"/>
    <dbReference type="OMA" id="HPIVYLN"/>
    <dbReference type="OrthoDB" id="6071166at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005518">
        <property type="term" value="F:collagen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038062">
        <property type="term" value="F:protein tyrosine kinase collagen receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010976">
        <property type="term" value="P:positive regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000007">
        <property type="entry name" value="Discoidin domain receptor tyrosine kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000053">
        <property type="entry name" value="Epithelial discoidin domain-containing receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000082">
        <property type="entry name" value="Epithelial discoidin domain-containing receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048525">
        <property type="entry name" value="DDR1-2_DS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002011">
        <property type="entry name" value="Tyr_kinase_rcpt_2_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF297">
        <property type="entry name" value="RECEPTOR PROTEIN-TYROSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21114">
        <property type="entry name" value="DDR1-2_DS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01285">
        <property type="entry name" value="FA58C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01286">
        <property type="entry name" value="FA58C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00239">
        <property type="entry name" value="RECEPTOR_TYR_KIN_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15 24 26" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 21" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540089" description="receptor protein-tyrosine kinase" evidence="21">
        <location>
            <begin position="16"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="410"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="23">
        <location>
            <begin position="22"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="569"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="382"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="385"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00061639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="AHH38743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_017307075.1"/>
        </source>
    </evidence>
    <sequence length="864" mass="98192" checksum="B8C350DDD058676C" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W5UBT2</accession>
    <name>W5UBT2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mothers against decapentaplegic homolog</fullName>
            <shortName evidence="7">MAD homolog</shortName>
            <shortName evidence="7">Mothers against DPP homolog</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">SMAD family member</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SMAD6</name>
        <name evidence="13" type="synonym">smad6b</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the dwarfin/SMAD family.</text>
    </comment>
    <dbReference type="EMBL" id="JT411127">
        <property type="protein sequence ID" value="AHH39315.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017321688.1">
        <property type="nucleotide sequence ID" value="XM_017466199.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UBT2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032185"/>
    <dbReference type="GeneID" id="108264565"/>
    <dbReference type="KEGG" id="ipu:108264565"/>
    <dbReference type="CTD" id="564395"/>
    <dbReference type="GeneTree" id="ENSGT00940000158146"/>
    <dbReference type="OMA" id="LLVCKVY"/>
    <dbReference type="OrthoDB" id="5946219at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071144">
        <property type="term" value="C:heteromeric SMAD protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070411">
        <property type="term" value="F:I-SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140416">
        <property type="term" value="F:transcription regulator inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060395">
        <property type="term" value="P:SMAD protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10493">
        <property type="entry name" value="MH1_SMAD_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10499">
        <property type="entry name" value="MH2_SMAD_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.10:FF:000004">
        <property type="entry name" value="Mothers against decapentaplegic homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.520.10:FF:000003">
        <property type="entry name" value="Mothers against decapentaplegic homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.520.10">
        <property type="entry name" value="SMAD MH1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013790">
        <property type="entry name" value="Dwarfin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003619">
        <property type="entry name" value="MAD_homology1_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013019">
        <property type="entry name" value="MAD_homology_MH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017855">
        <property type="entry name" value="SMAD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001132">
        <property type="entry name" value="SMAD_dom_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036578">
        <property type="entry name" value="SMAD_MH1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703:SF28">
        <property type="entry name" value="MOTHERS AGAINST DECAPENTAPLEGIC HOMOLOG 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703">
        <property type="entry name" value="SMAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03165">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03166">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00523">
        <property type="entry name" value="DWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00524">
        <property type="entry name" value="DWB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56366">
        <property type="entry name" value="SMAD MH1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51075">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51076">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MH1" evidence="9">
        <location>
            <begin position="124"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="MH2" evidence="10">
        <location>
            <begin position="320"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="14"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="113"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="33"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AHH39315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017321688.1"/>
        </source>
    </evidence>
    <sequence length="488" mass="53297" checksum="11C9A36538716FAC" modified="2014-04-16" version="1">MFRTKRSGLVRRLWRSRLVPDNEEGDGNGKSGDCGRDDVGEHPEKIGKTEFGAMTPSGSAGAELGGGVCSSAGWGVTLDQHGGAVVCVPERGSPRSAQDIDSRTVTCCLFKDRDSSGPGSTEAPTVARVKEPSSCHWAPRSPGSATVESPETKRRSFAEQELKAATYSLLKRLKEKTLDALLEAVESRGGVPSECVMVARGELRLGAHAAPPQLLVCKLYRWTDLQHTAQLKALYECKSFGGRDGPLVCCNPYHYSRLCGPESPPPPYSRLSPSDEHKPLDLSDSTLSYTEMEAASSPNITQGEFSDASLSPDAPKQSHWCSVAYWEHRTRVGRLYPVYEPSVSIFYDLPQGTGLCLGQLGGEHRGGTSQRTRSKIGYGLLLSKEPDGVWAYNRSQHPIFVNSPTLEAPGGRGLLVRKVMPGYSIKVFDYELSARLLLRHEAEPALPDGPYDPNSVRISFAKGWGPCYSRQFITSCPCWLEILLNNHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5UCI6</accession>
    <name>W5UCI6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="7">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">USP14</name>
        <name evidence="12" type="synonym">usp14</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family. USP14/UBP6 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7"/>
    <dbReference type="EMBL" id="JT411967">
        <property type="protein sequence ID" value="AHH39678.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047012508.1">
        <property type="nucleotide sequence ID" value="XM_047156552.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UCI6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007671"/>
    <dbReference type="GeneID" id="108267081"/>
    <dbReference type="KEGG" id="ipu:108267081"/>
    <dbReference type="GeneTree" id="ENSGT00390000009615"/>
    <dbReference type="OrthoDB" id="333239at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061136">
        <property type="term" value="P:regulation of proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02657">
        <property type="entry name" value="Peptidase_C19A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16104">
        <property type="entry name" value="Ubl_USP14_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000119">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000032">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044635">
        <property type="entry name" value="UBP14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43982">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43982:SF1">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="4"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="105"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU366025"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50053"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AHH39678.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_047012508.1"/>
        </source>
    </evidence>
    <sequence length="494" mass="56135" checksum="D275485B595E6AEA" modified="2014-04-16" version="1">MPVFTVNVKWGKEKFEEVELNTEEPPMVFKAQLFALTGVQPDRQKVMVKGGTLKDEEWGNIKIKSGMTFLMMGSADALPEEPSVRPMFVEDMTEEQLASAMELPCGLTNLGNTCYMNATVQCLRSVPELRTSLKKYSGALRSSGAGAPSQYITAALRDLYESMDKTSSSIPPIILLQFLHMAFPQFAEKGDQGQYLQQDANECWVQLMRVLQQKLESQEPETPMETGDVDGGAAASSAKKNFIDQFFGLEFDTTMKCTESEDEEPTKGTESQLQLSCFINQEVKYLATGLRLRLQEELTKFSPRLQRDALYSKTSKISRLPAYLTVQMVRFFYKEKESVNAKVLKDVKFPLMLDVYELCTAELQEKMVSVRSKFKDMEDKKLEKQQQKINKTVEAPKEVKYEDFSFPDDLGSNNSGYYDLQAVLTHQGRSSSSGHYLAWVKRKEDEWVKFDDDKVSVVTPEDILKLSGGGDWHIAYVLVYGPRRLEVMEEEQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5UCW3</accession>
    <name>W5UCW3_ICTPU</name>
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        <submittedName>
            <fullName evidence="19">Potassium voltage-gated channel subfamily H member 6a isoform X2</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="17">Potassium voltage-gated channel subfamily H member 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">KCNH7</name>
        <name evidence="19" type="synonym">kcnh6a</name>
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    <organism evidence="17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
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        <reaction evidence="13">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="molecule type" value="mRNA"/>
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        <property type="nucleotide sequence ID" value="XM_017494636.3"/>
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    <dbReference type="OMA" id="IFGEPNH"/>
    <dbReference type="OrthoDB" id="432483at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005242">
        <property type="term" value="F:inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086013">
        <property type="term" value="P:membrane repolarization during cardiac muscle cell action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086091">
        <property type="term" value="P:regulation of heart rate by cardiac conduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060307">
        <property type="term" value="P:regulation of ventricular cardiac muscle cell membrane repolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Potassium channel, voltage-gated eag-related subfamily H, member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.260:FF:000001">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000001">
        <property type="entry name" value="Potassium voltage-gated channel subfamily H member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003967">
        <property type="entry name" value="K_chnl_volt-dep_ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050818">
        <property type="entry name" value="KCNH_animal-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217:SF638">
        <property type="entry name" value="ERG K+ CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10217">
        <property type="entry name" value="VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01470">
        <property type="entry name" value="ERGCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="512"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="609"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="16">
        <location>
            <begin position="712"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="218"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="841"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1063"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1110"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="853"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="864"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1084"/>
            <end position="1096"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AHH39773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017350125.1"/>
        </source>
    </evidence>
    <sequence length="1172" mass="131270" checksum="BE49881091CBDDB0" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5UD55</accession>
    <name>W5UD55_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">2',5'-phosphodiesterase 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Mitochondrial deadenylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Pde12</name>
        <name evidence="19" type="synonym">pde12</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CCR4/nocturin family.</text>
    </comment>
    <dbReference type="EMBL" id="JT412522">
        <property type="protein sequence ID" value="AHH39883.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017306001.1">
        <property type="nucleotide sequence ID" value="XM_017450512.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UD55"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000010433"/>
    <dbReference type="GeneID" id="108254968"/>
    <dbReference type="KEGG" id="ipu:108254968"/>
    <dbReference type="CTD" id="201626"/>
    <dbReference type="OMA" id="FRLKSAC"/>
    <dbReference type="OrthoDB" id="412787at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000018">
        <property type="entry name" value="2',5'-phosphodiesterase 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048821">
        <property type="entry name" value="PDE12-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF37">
        <property type="entry name" value="2',5'-PHOSPHODIESTERASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21171">
        <property type="entry name" value="PDE12-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="2',5'-phosphodiesterase 12-like N-terminal" evidence="16">
        <location>
            <begin position="147"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="15">
        <location>
            <begin position="272"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00072755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF21171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AHH39883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017306001.1"/>
        </source>
    </evidence>
    <sequence length="584" mass="65553" checksum="63959AEE4030525C" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5UE22</accession>
    <name>W5UE22_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">KDEL motif-containing protein 1</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="15">Protein O-glucosyltransferase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">kdelc1</name>
        <name evidence="15" type="synonym">poglut2</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[EGF-like domain protein] + UDP-alpha-D-glucose = 3-O-(beta-D-glucosyl)-L-seryl-[EGF-like domain protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:58116"/>
            <dbReference type="Rhea" id="RHEA-COMP:14610"/>
            <dbReference type="Rhea" id="RHEA-COMP:16010"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:140576"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[EGF-like domain protein] + UDP-alpha-D-xylose = 3-O-(beta-D-xylosyl)-L-seryl-[EGF-like domain protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:62016"/>
            <dbReference type="Rhea" id="RHEA-COMP:16010"/>
            <dbReference type="Rhea" id="RHEA-COMP:16011"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132085"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KDELC family.</text>
    </comment>
    <dbReference type="EMBL" id="JT413183">
        <property type="protein sequence ID" value="AHH40145.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017325927.1">
        <property type="nucleotide sequence ID" value="XM_017470438.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UE22"/>
    <dbReference type="GeneID" id="108266749"/>
    <dbReference type="KEGG" id="ipu:108266749"/>
    <dbReference type="CTD" id="79070"/>
    <dbReference type="GeneTree" id="ENSGT00940000158318"/>
    <dbReference type="OMA" id="MFMDATL"/>
    <dbReference type="OrthoDB" id="541052at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046527">
        <property type="term" value="F:glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000419">
        <property type="entry name" value="KDEL (Lys-Asp-Glu-Leu) containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006598">
        <property type="entry name" value="CAP10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051091">
        <property type="entry name" value="O-Glucosyltr/Glycosyltrsf_90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203">
        <property type="entry name" value="KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203:SF21">
        <property type="entry name" value="PROTEIN O-GLUCOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05686">
        <property type="entry name" value="Glyco_transf_90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00672">
        <property type="entry name" value="CAP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540182" evidence="11">
        <location>
            <begin position="25"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="10">
        <location>
            <begin position="26"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase CAP10" evidence="12">
        <location>
            <begin position="228"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AHH40145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017325927.1"/>
        </source>
    </evidence>
    <sequence length="503" mass="58391" checksum="C846337F9D8B6368" modified="2014-04-16" version="1">MLWRALSGFVLYCTLSCLVTVAVCSSALPSASKSLVWGLGLEANAVLPARFFFIQSADKAGKNFTTSPGANTFEVKITSLSEPFSRIWVQVLDRQDGSFLVRYRMYASYTDLSIQILLKNKHVAKSPYILKGPVYHESCNCPEPSGALWEKHMHCPSSFSQIQYDLSFFPKVDLERNSHEIPQRFGQRQSLCHYTIKNNQVYIKTHGEHVGFRIFMDAFLLSLTRKVRLPDVEFFVNLGDWPLEKRRTSEKLHPIFSWCGSNDTRDIVMPTYDLTESILETMGRVSLDMMSVQANTGPEWNEKNSTAFWRGRDSRRERLELVKMARANPTLLDAAFTNFFFFKHDESLYGPLVKHVSFFDFFKYKYQINVDGTVAAYRMPYLLAGDSVVLKQDSIYYEHFYSQLQPWVHFIPFKADLSDLLEKIRWAKDHDEEARKIAQAAQQFARIHLMGDSIFCYYFKLFQEYAKLQVTEPKIRDDMELVEQPKDDLFPCYCHRTKGKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>W5UH65</accession>
    <accession>A0A2D0T796</accession>
    <name>W5UH65_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="25 27">Neurocan core protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">Ncan</name>
        <name evidence="27" type="synonym">ncana</name>
    </gene>
    <organism evidence="25">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="25">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">SDA103</strain>
        </source>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="27">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the HAPLN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="JT410326">
        <property type="protein sequence ID" value="AHH39012.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017350515.3">
        <property type="nucleotide sequence ID" value="XM_017495026.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UH65"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000028997"/>
    <dbReference type="GeneID" id="108280200"/>
    <dbReference type="KEGG" id="ipu:108280200"/>
    <dbReference type="CTD" id="407661"/>
    <dbReference type="OMA" id="FFQRHVP"/>
    <dbReference type="OrthoDB" id="5860362at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072534">
        <property type="term" value="C:perineuronal net"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005540">
        <property type="term" value="F:hyaluronic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010001">
        <property type="term" value="P:glial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002052">
        <property type="term" value="P:positive regulation of neuroblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd03517">
        <property type="entry name" value="Link_domain_CSPGs_modules_1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000001">
        <property type="entry name" value="Hyaluronan proteoglycan link protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000002">
        <property type="entry name" value="Hyaluronan proteoglycan link protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000003">
        <property type="entry name" value="Versican core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000003">
        <property type="entry name" value="Versican core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000006">
        <property type="entry name" value="Versican core protein-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050691">
        <property type="entry name" value="Hyaluronan_bind_Proteoglycan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000538">
        <property type="entry name" value="Link_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22804">
        <property type="entry name" value="AGGRECAN/VERSICAN PROTEOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22804:SF24">
        <property type="entry name" value="NEUROCAN CORE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00193">
        <property type="entry name" value="Xlink"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01265">
        <property type="entry name" value="LINKMODULE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00445">
        <property type="entry name" value="LINK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01241">
        <property type="entry name" value="LINK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50963">
        <property type="entry name" value="LINK_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 15" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0373">Hyaluronic acid</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5 16" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540278" evidence="19">
        <location>
            <begin position="29"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="22">
        <location>
            <begin position="52"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Link" evidence="24">
        <location>
            <begin position="151"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Link" evidence="24">
        <location>
            <begin position="252"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="20">
        <location>
            <begin position="776"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="20">
        <location>
            <begin position="811"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="21">
        <location>
            <begin position="860"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="23">
        <location>
            <begin position="978"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="345"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="397"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="524"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="577"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1040"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="348"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="403"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="431"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="524"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="799"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="837"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="980"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="1009"/>
            <end position="1036"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="AHH39012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_017350515.3"/>
        </source>
    </evidence>
    <sequence length="1070" mass="118217" checksum="764EE1F6E4F8CD7F" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>W5UK87</accession>
    <name>W5UK87_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 23">Protein kinase C</fullName>
            <ecNumber evidence="5 23">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="33" type="primary">PRKCA</name>
        <name evidence="35" type="synonym">prkcaa</name>
    </gene>
    <organism evidence="33">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="33">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="34" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="34">SDA103</strain>
        </source>
    </reference>
    <reference evidence="35" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="35">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21 23">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="27">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="27">Binds 3 Ca(2+) ions per subunit. The ions are bound to the C2 domain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 23">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="5 23"/>
    <dbReference type="EMBL" id="JT416689">
        <property type="protein sequence ID" value="AHH42115.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017337648.1">
        <property type="nucleotide sequence ID" value="XM_017482159.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UK87"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022638"/>
    <dbReference type="GeneID" id="108273127"/>
    <dbReference type="KEGG" id="ipu:108273127"/>
    <dbReference type="CTD" id="497384"/>
    <dbReference type="GeneTree" id="ENSGT00940000156104"/>
    <dbReference type="OMA" id="VHEIKSH"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20833">
        <property type="entry name" value="C1_cPKC_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20836">
        <property type="entry name" value="C1_cPKC_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04026">
        <property type="entry name" value="C2_PKC_alpha_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000012">
        <property type="entry name" value="Kinase C alpha type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000023">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000080">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000103">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000006">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000031">
        <property type="entry name" value="Protein kinase C alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014375">
        <property type="entry name" value="Protein_kinase_C_a/b/g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000550">
        <property type="entry name" value="PKC_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="10" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="18 23 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17 27" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15 23 33" id="KW-0418">Kinase</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 27" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 23 28" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 23" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 23" id="KW-0808">Transferase</keyword>
    <keyword evidence="16" id="KW-0862">Zinc</keyword>
    <keyword evidence="14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="31">
        <location>
            <begin position="32"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="31">
        <location>
            <begin position="97"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="29">
        <location>
            <begin position="154"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="30">
        <location>
            <begin position="335"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="32">
        <location>
            <begin position="594"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="24">
        <location>
            <position position="459"/>
        </location>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <begin position="341"/>
            <end position="349"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26 28">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRNR" id="PIRNR000550"/>
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    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR000550-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR000550-2"/>
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    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR000550-3"/>
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    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR000550-4"/>
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    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
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    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
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    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="EMBL" id="AHH42115.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="RefSeq" id="XP_017337648.1"/>
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    </evidence>
    <sequence length="668" mass="76295" checksum="EBFB439460B424F6" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5UL27</accession>
    <name>W5UL27_ICTPU</name>
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        <submittedName>
            <fullName evidence="9 11">Differentially expressed in FDCP 8</fullName>
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    <gene>
        <name evidence="9" type="primary">DEF8</name>
        <name evidence="11" type="synonym">def8</name>
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    <organism evidence="9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts. Involved in bone resorption.</text>
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    <comment type="similarity">
        <text evidence="5">Belongs to the DEF8 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017466336.3"/>
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        <property type="component" value="Chromosome 4"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="CDD" id="cd20819">
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        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
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    <dbReference type="InterPro" id="IPR051366">
        <property type="entry name" value="DEF8"/>
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    <dbReference type="InterPro" id="IPR047983">
        <property type="entry name" value="DEF8_C1"/>
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    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
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    <dbReference type="InterPro" id="IPR025258">
        <property type="entry name" value="RH_dom"/>
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    <dbReference type="PANTHER" id="PTHR12326:SF3">
        <property type="entry name" value="DIFFERENTIALLY EXPRESSED IN FDCP 8 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12326">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF13901">
        <property type="entry name" value="RH_dom"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DUF4206"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
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    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="8">
        <location>
            <begin position="132"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AHH42777.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017321825.1"/>
        </source>
    </evidence>
    <sequence length="439" mass="51419" checksum="DFEA6D1E16A63431" modified="2014-04-16" version="1">MEYDERLARFRQGHVNPFDRSESDGTSDRKDPLKQEVRPELFSTETKIQNSDRTMDLGLAEDHFSRPVGSFVASDIEQLKQAIEECKKLILELPEHSERQKDTVAKLIHLRLKLQELKDPEEDEPNLRILLEHRFSKEKSKSVKQTCDKCSTIIWGLIQTWYTCTGCYYRCHSKCMNLITKQCVRSKVSHQSEYELNICPEIGLDKQDYRCAECRAQISLRGIPSEARQCDYTGQYYCNSCHWNDTAIVPARVIHNWEFEPKKVCRSSMRYLTLMISRPVLKLKEINPLLFNFVEELVEIRKLRQDILLMKPYFITCKEAMEARLLLQLQDRQHFVENDEMYSLQDLIDISSGRLSCSLTEIHTTFAKHIKLDCERCQAKGFVCELCKEGDILFPFDSHTSVCHDCSAVFHRDCYYDNSMTCPRCARMTERKQDELAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5UQQ4</accession>
    <name>W5UQQ4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10 12">Arylsulfatase B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ARSB</name>
        <name evidence="12" type="synonym">LOC108279373</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfatase family.</text>
    </comment>
    <dbReference type="EMBL" id="JT415954">
        <property type="protein sequence ID" value="AHH41878.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017349082.1">
        <property type="nucleotide sequence ID" value="XM_017493593.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UQQ4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003783"/>
    <dbReference type="GeneID" id="108279373"/>
    <dbReference type="KEGG" id="ipu:108279373"/>
    <dbReference type="GeneTree" id="ENSGT00940000158270"/>
    <dbReference type="OMA" id="HEHCVFI"/>
    <dbReference type="OrthoDB" id="103349at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008484">
        <property type="term" value="F:sulfuric ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16029">
        <property type="entry name" value="4-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000007">
        <property type="entry name" value="Arylsulfatase family, member J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047115">
        <property type="entry name" value="ARSB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10342">
        <property type="entry name" value="ARYLSULFATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10342:SF274">
        <property type="entry name" value="ARYLSULFATASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00523">
        <property type="entry name" value="SULFATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00149">
        <property type="entry name" value="SULFATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540406" evidence="8">
        <location>
            <begin position="22"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase N-terminal" evidence="9">
        <location>
            <begin position="25"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="504"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AHH41878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017349082.1"/>
        </source>
    </evidence>
    <sequence length="527" mass="59334" checksum="31ACF33FE1DBA743" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PKS0</accession>
    <name>A0A2D0PKS0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">G-protein coupled receptor-associated protein LMBRD2B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">lmbrd2a</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LIMR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017307212.1">
        <property type="nucleotide sequence ID" value="XM_017451723.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017307213.1">
        <property type="nucleotide sequence ID" value="XM_017451724.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PKS0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000001350"/>
    <dbReference type="GeneID" id="108255635"/>
    <dbReference type="KEGG" id="ipu:108255635"/>
    <dbReference type="CTD" id="550238"/>
    <dbReference type="OrthoDB" id="203099at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071875">
        <property type="term" value="P:adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051584">
        <property type="entry name" value="GPCR-associated_LMBR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006876">
        <property type="entry name" value="LMBR1-like_membr_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21355">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR-ASSOCIATED PROTEIN LMBRD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21355:SF0">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR-ASSOCIATED PROTEIN LMBRD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04791">
        <property type="entry name" value="LMBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="386"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="433"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="531"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="232"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017307212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017307213.1"/>
        </source>
    </evidence>
    <sequence length="671" mass="78222" checksum="9161E3845144AEFA" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PQ37</accession>
    <name>A0A2D0PQ37_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Corticotropin releasing hormone b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">crhb</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017308129.1">
        <property type="nucleotide sequence ID" value="XM_017452640.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PQ37"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021228"/>
    <dbReference type="GeneID" id="108256093"/>
    <dbReference type="KEGG" id="ipu:108256093"/>
    <dbReference type="CTD" id="492507"/>
    <dbReference type="OMA" id="EMMEMSK"/>
    <dbReference type="OrthoDB" id="9837731at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018446">
        <property type="entry name" value="Corticotropin-releasing_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000187">
        <property type="entry name" value="CRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003620">
        <property type="entry name" value="Urocortin_CRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15035:SF9">
        <property type="entry name" value="CORTICOLIBERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15035">
        <property type="entry name" value="CORTICOLIBERIN/UROCORTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00473">
        <property type="entry name" value="CRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01612">
        <property type="entry name" value="CRFFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00039">
        <property type="entry name" value="CRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00511">
        <property type="entry name" value="CRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0027">Amidation</keyword>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="5" id="KW-0372">Hormone</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012858663" evidence="9">
        <location>
            <begin position="27"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Corticotropin-releasing factor" evidence="10">
        <location>
            <begin position="129"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="72"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017308129.1"/>
        </source>
    </evidence>
    <sequence length="170" mass="19630" checksum="A70B689D85CAC011" modified="2017-12-20" version="1">MKLHFLVTTAAALLVAFPPRMNECRAVDGPDRRASLSSPSANALEEEEDQRRTRPIWARLGEEYFIRVGNRNQRERESEPETRPEPASAVFKRALQLRLTQHLLRQDHEVAEAARELRDAPAERPRRAEEPPISLDLTFHLLREVLEMAKAEQMAQQAHSNRKMMEIFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PQF7</accession>
    <name>A0A2D0PQF7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Leucine-rich repeat-containing protein 34</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">lrrc34</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017308818.2">
        <property type="nucleotide sequence ID" value="XM_017453329.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PQF7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000025279"/>
    <dbReference type="GeneID" id="108256442"/>
    <dbReference type="KEGG" id="ipu:108256442"/>
    <dbReference type="CTD" id="151827"/>
    <dbReference type="OrthoDB" id="272549at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052201">
        <property type="entry name" value="LRR-containing_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24111">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24111:SF4">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017308818.2"/>
        </source>
    </evidence>
    <sequence length="432" mass="48199" checksum="9F5207D83EAF1DC0" modified="2023-02-22" version="2">MGTHNQSCVFSLSHFRSIVSMESLNNLYSSICSELQLSLNTYISQELKETEKCDRDVSLKLRGNNRLKQVFRLTDEDVKALSKTLRNNLHVKALDLRYNKITNEGAVHLADLIQENVALQELDLMCNSIEAGGAERIAKSLHHNTTLKKLRMTGNKIGNKGAMHFASMLQINTTLEELDVSDCDLDTQSLITFAIVLSNNRSLVSINVSRPLLFSLQDETTVHTAHMLEVNRSLKELHMGKHSMTDSGVQRLCEALISNHSLRYLDLRCNRITRDGARCLAGLLRNNDTIQILDLSFNRIEDDGAVYLSDAIALKHTKLRALSIQSNNVSTTGLLSLREAINANPHLTHIYIWGNRLEEPVCEAFSQLISSSRLSEQHTDVTPYTADGRVHLAEVSNGLCRHYYWTPSYSEDGGAASNAALTLNITPTHTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PRE4</accession>
    <name>A0A2D0PRE4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">E3 ubiquitin-protein ligase ZNRF3</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">RING-type E3 ubiquitin transferase ZNRF3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Zinc/RING finger protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">znrf3</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 25">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ZNRF3 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017307650.2">
        <property type="nucleotide sequence ID" value="XM_017452161.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PRE4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026950"/>
    <dbReference type="GeneID" id="108255857"/>
    <dbReference type="KEGG" id="ipu:108255857"/>
    <dbReference type="CTD" id="84133"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16799">
        <property type="entry name" value="RING-H2_ZNRF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000018">
        <property type="entry name" value="E3 ubiquitin-protein ligase ZNRF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000075">
        <property type="entry name" value="Putative e3 ubiquitin-protein ligase rnf43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040700">
        <property type="entry name" value="ZNRF-3_ecto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051073">
        <property type="entry name" value="ZNRF3_Arkadia_E3_ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045903">
        <property type="entry name" value="ZNRF3_Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16200">
        <property type="entry name" value="RING ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18212">
        <property type="entry name" value="ZNRF_3_ecto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0879">Wnt signaling pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="12 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="22">
        <location>
            <begin position="295"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00077187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_017307650.2"/>
        </source>
    </evidence>
    <sequence length="876" mass="96086" checksum="51883DD1853B3C40" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PSC6</accession>
    <name>A0A2D0PSC6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Riboflavin transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">si:ch73-196l6.5</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Plasma membrane transporter mediating the uptake by cells of the water soluble vitamin B2/riboflavin that plays a key role in biochemical oxidation-reduction reactions of the carbohydrate, lipid, and amino acid metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>riboflavin(in) = riboflavin(out)</text>
            <dbReference type="Rhea" id="RHEA:35015"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cell membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the riboflavin transporter family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017309463.2">
        <property type="nucleotide sequence ID" value="XM_017453974.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PSC6"/>
    <dbReference type="GeneID" id="108256769"/>
    <dbReference type="KEGG" id="ipu:108256769"/>
    <dbReference type="CTD" id="555631"/>
    <dbReference type="OrthoDB" id="9995836at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032217">
        <property type="term" value="F:riboflavin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009357">
        <property type="entry name" value="Riboflavin_transptr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12929:SF19">
        <property type="entry name" value="RIBOFLAVIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12929">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06237">
        <property type="entry name" value="SLC52_ribofla_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="404"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="437"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="470"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017309463.2"/>
        </source>
    </evidence>
    <sequence length="509" mass="56183" checksum="F44EF112E447D136" modified="2023-02-22" version="1">MLVLISGGSIIKCVHLLIKHQQAGHHGWSVNAHLQSQTKVNTLLSCQMTLTNEDSKQIKDMSLLTHVLACLFGMGSWVTICGLWVELPLIVPQVPEGWYLPSYLSILTQVANVGPLFVTLMHRFRPGKLNETAVIYFIVCFGTLASVLLAFLWKETVIVQGVERSIYLLILTFFISTVDCTSSVTFLPFMMQLKAEYLTTYYVGEGLSGLVPAVVALIQGVGAMHCVNSSQNFNPNETISGSLDSANYLIPHYLPANFSAKAFFFFLAAMMFVCFVAFLLLNKLPVVARERSISLKYQGAIRMSDFVREKKNSEQKPMMKSPNVIKKFSGMYGKGIYSWAQIVYIFIILAWVNALTNSVLLSVQSYSCLPYGNRAYHWSATLASLANPLACFIAMFYAQRSLVLMGFLTAFGSVIGVYIMGMAVLSPCPLLVNHISGAILIVAAWTFFVLTLSYVKVIIAVILRDEGHSALVWCGAVVQLGSLLGAVIMFPLVNVYDFFSSGDPCNTRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PT26</accession>
    <name>A0A2D0PT26_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="5">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20 21 22 23" type="primary">otud7b</name>
    </gene>
    <organism evidence="19 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20 21 22" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20 21 22">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase C64 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017308238.1">
        <property type="nucleotide sequence ID" value="XM_017452749.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017308239.1">
        <property type="nucleotide sequence ID" value="XM_017452750.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017308240.1">
        <property type="nucleotide sequence ID" value="XM_017452751.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053530939.1">
        <property type="nucleotide sequence ID" value="XM_053674964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PT26"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009474"/>
    <dbReference type="GeneID" id="108256168"/>
    <dbReference type="KEGG" id="ipu:108256168"/>
    <dbReference type="CTD" id="56957"/>
    <dbReference type="OMA" id="KEWEWGK"/>
    <dbReference type="OrthoDB" id="10064699at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070530">
        <property type="term" value="F:K63-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071947">
        <property type="term" value="P:protein deubiquitination involved in ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035871">
        <property type="term" value="P:protein K11-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070536">
        <property type="term" value="P:protein K63-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22772">
        <property type="entry name" value="OTU_OTUD7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14347">
        <property type="entry name" value="UBA_Cezanne_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000017">
        <property type="entry name" value="OTU domain-containing protein 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051346">
        <property type="entry name" value="OTU_Deubiquitinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054109">
        <property type="entry name" value="UBA_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002653">
        <property type="entry name" value="Znf_A20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13367:SF8">
        <property type="entry name" value="OTU DOMAIN-CONTAINING PROTEIN 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13367">
        <property type="entry name" value="UBIQUITIN THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22566">
        <property type="entry name" value="UBA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51036">
        <property type="entry name" value="ZF_A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="OTU" evidence="17">
        <location>
            <begin position="180"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="A20-type" evidence="18">
        <location>
            <begin position="864"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="439"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="674"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="836"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="452"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="468"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="507"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="532"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="565"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="601"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="674"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="704"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017308238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017308239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017308240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_053530939.1"/>
        </source>
    </evidence>
    <sequence length="907" mass="99066" checksum="79682004066F8BDA" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PW22</accession>
    <name>A0A2D0PW22_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Homeobox-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">zgc:91944</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">SDA103</strain>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Binds directly to 5'-TTAGGG-3' repeats in telomeric DNA. Associates with the telomerase complex at sites of active telomere processing and positively regulates telomere elongation. Important for TERT binding to chromatin, indicating a role in recruitment of the telomerase complex to telomeres. Also plays a role in the alternative lengthening of telomeres (ALT) pathway in telomerase-negative cells where it promotes formation and/or maintenance of ALT-associated promyelocytic leukemia bodies (APBs). Enhances formation of telomere C-circles in ALT cells, suggesting a possible role in telomere recombination. Might also be involved in the DNA damage response at telomeres.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Associates with the telomerase holoenzyme complex. Interacts with DKC1, XRCC6 and COIL.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Chromosome</location>
            <location evidence="4">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Cajal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">PML body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017310217.1">
        <property type="nucleotide sequence ID" value="XM_017454728.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PW22"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000002214"/>
    <dbReference type="GeneID" id="108257190"/>
    <dbReference type="KEGG" id="ipu:108257190"/>
    <dbReference type="OMA" id="CCEAEPR"/>
    <dbReference type="OrthoDB" id="5856131at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003691">
        <property type="term" value="F:double-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000038">
        <property type="entry name" value="Homeobox-containing protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000011">
        <property type="entry name" value="homeobox-containing protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040363">
        <property type="entry name" value="HMBOX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006899">
        <property type="entry name" value="HNF-1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044869">
        <property type="entry name" value="HNF-1_POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044866">
        <property type="entry name" value="HNF_P1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
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        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
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        <property type="entry name" value="HOMEOBOX-CONTAINING PROTEIN 1"/>
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        <property type="entry name" value="HOMEODOX-CONTAINING PROTEIN 1 HMBOX1"/>
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    </dbReference>
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        <property type="entry name" value="HNF-1_N"/>
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        <property type="entry name" value="Homeodomain"/>
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        <property type="entry name" value="HOX"/>
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        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
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    <keyword evidence="13 19 20" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
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    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
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        <location>
            <begin position="35"/>
            <end position="66"/>
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        <location>
            <begin position="141"/>
            <end position="237"/>
        </location>
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    <feature type="domain" description="Homeobox" evidence="22">
        <location>
            <begin position="303"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="19">
        <location>
            <begin position="305"/>
            <end position="379"/>
        </location>
    </feature>
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            <begin position="73"/>
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        </location>
    </feature>
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        <location>
            <begin position="429"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="83"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="454"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00059255"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00064808"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00072740"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51936"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51937"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_017310217.1"/>
        </source>
    </evidence>
    <sequence length="469" mass="53221" checksum="9CF579A5FEBC67A1" modified="2017-12-20" version="1">MRQWSLRAVAPRAEPLPTGRVSPQLSDARMECCDVEPRYTIEQIDLLQRLRLSGLTKPQILQALESLERLDPEHRSPFCDNHSAPPRAPTSAAPAAPSSSSSSSSSLTSATTQTPVLDAALSPSNSYDASSPPLYPPGVQRSFAYDLAEEDWDLEEKVEEYMRRDSNLVKEEIKAFLNNRRISQAIVGQVTGISQSYISQWLLQQGLEMSDSKRRAFYRWYLLERNSPGFRWTENQHAQGPRSRTDTPWSRQRELLMHLLPWYSAQQAQPGATLSMRSLVKEEPDWRVAGCPGDRAAVGGPFRLRRGSRFTWRKECQSIMESFFIENQYPDEAKREEIANTCNSVIQKPGCKLSEFERVTALKVYNWFANRRKEMKRRANIEAAILESHGIEVPSPSCHSNGEEVENQEFGDQVVNQRFPDQEELAQRKVTEPDGATLPAVEVVPLPSPATQHMDQKMDETKREAADEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PX55</accession>
    <name>A0A2D0PX55_ICTPU</name>
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        <submittedName>
            <fullName evidence="4">Pdgfa associated protein 1b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pdap1b</name>
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    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017310607.1">
        <property type="nucleotide sequence ID" value="XM_017455118.3"/>
    </dbReference>
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    <dbReference type="STRING" id="7998.ENSIPUP00000025292"/>
    <dbReference type="GeneID" id="108257385"/>
    <dbReference type="KEGG" id="ipu:108257385"/>
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    <dbReference type="OMA" id="IDMESPR"/>
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        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019380">
        <property type="entry name" value="Casein_kinase_sb_PP28"/>
    </dbReference>
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        <property type="entry name" value="HAP28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22055">
        <property type="entry name" value="28 KDA HEAT- AND ACID-STABLE PHOSPHOPROTEIN PDGF-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="80"/>
            <end position="157"/>
        </location>
    </feature>
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            <begin position="1"/>
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            <begin position="161"/>
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        </location>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF10252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017310607.1"/>
        </source>
    </evidence>
    <sequence length="178" mass="19964" checksum="B29B27A55F14DD0F" modified="2017-12-20" version="1">MPKGGKKGGHKGRMRTYTSPEEIDAQMKAEKERKKNEEAEEGGAVNDMKDDKLAGSGSEDSDDESSHKRKGVEGLIDIENPNRAAQKTKKVTELELEEPKQLSRREREEIEKQKAKERYMKMHLAGKTDQAKADLARLAIIRKQREEAAKKKEEERKAKEAAQAAQAAAKGLHTLSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PZ54</accession>
    <name>A0A2D0PZ54_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Vesicle-associated membrane protein 4 isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">vamp4</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017311329.1">
        <property type="nucleotide sequence ID" value="XM_017455840.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PZ54"/>
    <dbReference type="GeneID" id="108257792"/>
    <dbReference type="CTD" id="8674"/>
    <dbReference type="OrthoDB" id="190375at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090161">
        <property type="term" value="P:Golgi ribbon formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035493">
        <property type="term" value="P:SNARE complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15869">
        <property type="entry name" value="R-SNARE_VAMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000004">
        <property type="entry name" value="Vesicle-associated membrane protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042887">
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        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN 4"/>
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        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN 4"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00417">
        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="52"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017311329.1"/>
        </source>
    </evidence>
    <sequence length="140" mass="16413" checksum="547E9D9392142A0A" modified="2017-12-20" version="1">MPPKFKRHLNDDEVTGSVRSERRNLLEEDSDEEEDFFLRGPTGPRFGPQNDKIRQVQSQVDEVIDVMQENISKVIERGERLEDLQDKSESLSDNASAFSSRAKQLHRRMWWRDIKMKMVVALVIVVLLLIIIIPVILRYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PZV6</accession>
    <name>A0A2D0PZV6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 21">Clusterin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">clu</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">SDA103</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="27">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Secretory vesicle</location>
            <location evidence="3">Chromaffin granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Mitochondrion membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 21">Belongs to the clusterin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017312023.1">
        <property type="nucleotide sequence ID" value="XM_017456534.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PZV6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008397"/>
    <dbReference type="GeneID" id="108258125"/>
    <dbReference type="KEGG" id="ipu:108258125"/>
    <dbReference type="CTD" id="1191"/>
    <dbReference type="OMA" id="QGSKYIN"/>
    <dbReference type="OrthoDB" id="9018825at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042583">
        <property type="term" value="C:chromaffin granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000753">
        <property type="entry name" value="Clusterin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016015">
        <property type="entry name" value="Clusterin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033986">
        <property type="entry name" value="Clusterin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016014">
        <property type="entry name" value="Clusterin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10970">
        <property type="entry name" value="CLUSTERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10970:SF1">
        <property type="entry name" value="CLUSTERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01093">
        <property type="entry name" value="Clusterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00035">
        <property type="entry name" value="CLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00030">
        <property type="entry name" value="CLb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00492">
        <property type="entry name" value="CLUSTERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0143">Chaperone</keyword>
    <keyword evidence="22" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="20" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0964">Secreted</keyword>
    <keyword evidence="11 23" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012564874" description="Clusterin" evidence="23">
        <location>
            <begin position="20"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Clusterin N-terminal" evidence="24">
        <location>
            <begin position="24"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Clusterin C-terminal" evidence="25">
        <location>
            <begin position="213"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="22">
        <location>
            <begin position="57"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="22">
        <location>
            <begin position="320"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00010069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00020334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="SMART" id="SM00030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="SMART" id="SM00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_017312023.1"/>
        </source>
    </evidence>
    <sequence length="442" mass="51064" checksum="B3B096A56593EBFC" modified="2017-12-20" version="1">MRACWFLLGLSLLYISAECLLSPSTDDLKKISQLGEKYVDKEIENAINGVKKMKTVMERSEEDHKKFLSSLDETKKQKEDALKVAQEMEEKLNEEQSVCNETTQALWEECKPCLKNTCIKYYSRTCSSGSGLVGRQLEEFLNRSSPFSIWINGQNIDTLEKEDQQQRRHFQDLERRYTDVADGVDSIFVDSMKVFDHMRSLHQPGWFHRPSPIYRSLFHDPQFPDFHSMFQPMRQMTQDVFGSIGLYMGGDSNFPSEDGSVNEDVIITRPFGNDKMTCREIRRNSAGCIKLREECEKCKEIQHIDCSGKKPLEGPLKEDLEQALALAERFTKEYNNLLRRFEEEMFNTSKLLDTLRNQFSWVSSLANHTTSDDGIFKIQTIICKDTENPETPGDTNVSVKLFDEPEMSFTVPGEIPWTDPKFSEVVAQEALDRYKHNTIIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q1E5</accession>
    <name>A0A2D0Q1E5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Chloride intracellular channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">clic5b</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Membrane</location>
            <topology evidence="16">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="16">Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the chloride channel CLIC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017312107.1">
        <property type="nucleotide sequence ID" value="XM_017456618.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q1E5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027703"/>
    <dbReference type="GeneID" id="108258161"/>
    <dbReference type="KEGG" id="ipu:108258161"/>
    <dbReference type="CTD" id="405833"/>
    <dbReference type="OrthoDB" id="1935530at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10297">
        <property type="entry name" value="GST_C_CLIC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03061">
        <property type="entry name" value="GST_N_CLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000001">
        <property type="entry name" value="Chloride intracellular channel 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000021">
        <property type="entry name" value="Chloride intracellular channel 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002946">
        <property type="entry name" value="CLIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042069">
        <property type="entry name" value="CLIC5_C_GST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053823">
        <property type="entry name" value="CLIC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00862">
        <property type="entry name" value="O-ClC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45476:SF4">
        <property type="entry name" value="CHLORIDE INTRACELLULAR CHANNEL PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45476">
        <property type="entry name" value="CHLORIDE INTRACELLULAR CHANNEL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22441">
        <property type="entry name" value="CLIC-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01263">
        <property type="entry name" value="INTCLCHANNEL"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13 16" id="KW-0868">Chloride</keyword>
    <keyword evidence="12 16" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="5 16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10 16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <keyword evidence="7 16" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="domain" description="GST C-terminal" evidence="18">
        <location>
            <begin position="244"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="60"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="18"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017312107.1"/>
        </source>
    </evidence>
    <sequence length="416" mass="46579" checksum="30F5A3F6903C782A" modified="2017-12-20" version="1">MAEENIYEKIDEGNLYEKPFDEPDVSVKEEPKYENQSAVSYDTPETDALYCEIDVQSKAGRSLSPDYMNTNESTAEAEENSSEVQNGVGLYEVPAGENGEEGSSRHSSTLSSDHFGEPCDEEQLQESIYEDNAEDGMMMAFSLPKGRGAEPDEEVVDYSLKPVQNNTVEEEEKPVDPSLPEIALFVKAGSDGESIGNCPFSQRLFMILWLKGVVFNVTTVDLKRKPADLHNLAPGTHPPFLTYNGEVKTDINKIEEFLEEVLAPPKYPKLAAKHRESNTAGIDIFAKFSAYIKNSRPEANGGLEKNVTKALQKLDDYLNSALPEEIDADSMEEEKSSNRKFLDGNELTLADCNLLPKLHIVKVVAKKYRNYEIPTNLTGVWRYLNSAYDRDEFINTCPAEKEIELAYLDVAKRLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q1I2</accession>
    <name>A0A2D0Q1I2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Hairy/enhancer-of-split related with YRPW motif protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">hey2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the HEY family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017312159.1">
        <property type="nucleotide sequence ID" value="XM_017456670.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q1I2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008061"/>
    <dbReference type="GeneID" id="108258193"/>
    <dbReference type="KEGG" id="ipu:108258193"/>
    <dbReference type="CTD" id="23493"/>
    <dbReference type="OMA" id="DTTPCRL"/>
    <dbReference type="OrthoDB" id="6371181at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18920">
        <property type="entry name" value="bHLH-O_HEY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000012">
        <property type="entry name" value="hairy/enhancer-of-split related with YRPW motif protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07527">
        <property type="entry name" value="Hairy_orange"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00511">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158457">
        <property type="entry name" value="Orange domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="11">
        <location>
            <begin position="48"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="12">
        <location>
            <begin position="125"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="251"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="251"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017312159.1"/>
        </source>
    </evidence>
    <sequence length="289" mass="31063" checksum="425C4E95B75FFDC8" modified="2017-12-20" version="1">MKRPCEDTTSDSDMDETIDVGSENNYPAQSSSSFLRCASPTTTSQVMARKKRRGIIEKRRRDRINNSLSELRRLVPTAFEKQGSAKLEKAEILQMTVDHLKMLQATGGKGYFDFHSLAMDFMSIGFRECLTEVARYLSSVEGLDSSDPLRVRLVSHLSSCAAQLVASSITHHPQALHPLHPLHQLPAAFVARATPSASSSSSSSEPHRSSFSHSSSIAPPLSTSLLSLSATFPLSFPAGFPLFAPGMTSSATISPSISSSSTSSTSQQSSSGSSSAKPYRPWGTEVGAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q1P1</accession>
    <name>A0A2D0Q1P1_ICTPU</name>
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        <recommendedName>
            <fullName evidence="7">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tm9sf2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017312653.2">
        <property type="nucleotide sequence ID" value="XM_017457164.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q1P1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032634"/>
    <dbReference type="GeneID" id="108258490"/>
    <dbReference type="KEGG" id="ipu:108258490"/>
    <dbReference type="CTD" id="9375"/>
    <dbReference type="OrthoDB" id="1666796at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF111">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="396"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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    <feature type="transmembrane region" description="Helical" evidence="7">
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        <location>
            <begin position="623"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017312653.2"/>
        </source>
    </evidence>
    <sequence length="662" mass="75517" checksum="20D145293AB68F3B" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q1X8</accession>
    <name>A0A2D0Q1X8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ectonucleotide pyrophosphatase/phosphodiesterase family member 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">zgc:153896</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017311691.2">
        <property type="nucleotide sequence ID" value="XM_017456202.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q1X8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029576"/>
    <dbReference type="GeneID" id="108257993"/>
    <dbReference type="KEGG" id="ipu:108257993"/>
    <dbReference type="OrthoDB" id="415411at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="CDD" id="cd16018">
        <property type="entry name" value="Enpp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF65">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE_PHOSPHODIESTERASE FAMILY MEMBER 7-LIKE PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039936540" evidence="1">
        <location>
            <begin position="24"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017311691.2"/>
        </source>
    </evidence>
    <sequence length="447" mass="50692" checksum="37A2C3ACD405E382" modified="2023-06-28" version="2">MNPSVNMKLELLLLFAVLLCVDGVPLSSTGRRKHNKLLLISFDGFRWNYDEDVHTPNMDLLVREGVKAKYITPPMITMTSPSHFTTITGRWIEDHGVVHNMMFNSTTLLKLTHKNTLKRSVWWDNGALPLWITAQNQGLKTASYFYPGGGVNYSGQAVDRSLVEAEGHPDANETEWRLKIDIVLGWFTKENFDFVTLYYGEPDNVGHKVGPETEERKKIIEQIDRLIGYLRESIQKNDLADQLNVIITSDHGMTTIKKSPAVKEIKLSEYMNFVQLTHFDILDYGGFGMIRPKVGKVQEVYDKLKNAHPNLTVYKKEEIPENFHIAKNSRIQDLVLIGDLGFNLNSRIIFYVNKGDHGFSNHEMDMKTIFRAFGPDFRKNFLAEPFDSVSIYPLMCKLLGVDPAPNNGSLSDTQAMLVENSDPGGHAGRIRTSVGLVVITMFILRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q395</accession>
    <name>A0A2D0Q395_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tuberin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">tsc2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. Within the TSC-TBC complex, TSC2 acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. In absence of nutrients, the TSC-TBC complex inhibits mTORC1, thereby preventing phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) by the mTORC1 signaling. The TSC-TBC complex is inactivated in response to nutrients, relieving inhibition of mTORC1. Involved in microtubule-mediated protein transport via its ability to regulate mTORC1 signaling. Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Lysosome membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
        </subcellularLocation>
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        <property type="nucleotide sequence ID" value="XM_017457290.3"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:nucleus"/>
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    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0033596">
        <property type="term" value="C:TSC1-TSC2 complex"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:GTPase activator activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:negative regulation of insulin receptor signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
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        <property type="term" value="P:negative regulation of TOR signaling"/>
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        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:regulation of cell cycle"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0016241">
        <property type="term" value="P:regulation of macroautophagy"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Tuberin isoform X3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ARM-like"/>
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        <property type="entry name" value="ARM-type_fold"/>
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    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
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        <property type="entry name" value="Rap/Ran-GAP_sf"/>
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        <property type="entry name" value="Rap/Ran_GAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003913">
        <property type="entry name" value="Tuberin"/>
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        <property type="entry name" value="Tuberin-type_domain"/>
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    <dbReference type="InterPro" id="IPR027107">
        <property type="entry name" value="Tuberin/Ral-act_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024584">
        <property type="entry name" value="Tuberin_N"/>
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    <dbReference type="PANTHER" id="PTHR10063">
        <property type="entry name" value="TUBERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10063:SF0">
        <property type="entry name" value="TUBERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11864">
        <property type="entry name" value="DUF3384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02145">
        <property type="entry name" value="Rap_GAP"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF03542">
        <property type="entry name" value="Tuberin"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR01431">
        <property type="entry name" value="TUBERIN"/>
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        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111347">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAPGAP"/>
        <property type="match status" value="1"/>
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    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
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    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
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        <location>
            <begin position="488"/>
            <end position="526"/>
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    <feature type="domain" description="Rap-GAP" evidence="13">
        <location>
            <begin position="1575"/>
            <end position="1804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="653"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1075"/>
            <end position="1123"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1145"/>
            <end position="1185"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1251"/>
            <end position="1274"/>
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    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1314"/>
            <end position="1412"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1424"/>
            <end position="1467"/>
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    <feature type="region of interest" description="Disordered" evidence="12">
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            <begin position="1483"/>
            <end position="1532"/>
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    <feature type="region of interest" description="Disordered" evidence="12">
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            <begin position="1804"/>
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        <location>
            <begin position="658"/>
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        <location>
            <begin position="1092"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1111"/>
            <end position="1120"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1254"/>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1349"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1399"/>
            <end position="1412"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
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            <end position="1526"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1806"/>
            <end position="1838"/>
        </location>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023765"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00054764"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00070662"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017312779.1"/>
        </source>
    </evidence>
    <sequence length="1855" mass="205656" checksum="15C274223EB18B90" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q3F3</accession>
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        <recommendedName>
            <fullName evidence="3">LIM and SH3 domain protein 1</fullName>
        </recommendedName>
    </protein>
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        <name evidence="24" type="primary">lasp1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
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    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017312834.1">
        <property type="nucleotide sequence ID" value="XM_017457345.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A2D0Q3F3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034609"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="CDD" id="cd09447">
        <property type="entry name" value="LIM_LASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11934">
        <property type="entry name" value="SH3_Lasp1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000087">
        <property type="entry name" value="LIM zinc-binding domain-containing Nebulette"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000007">
        <property type="entry name" value="nebulin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035630">
        <property type="entry name" value="Lasp1_SH3"/>
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    <dbReference type="InterPro" id="IPR051759">
        <property type="entry name" value="LIM-SH3_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000900">
        <property type="entry name" value="Nebulin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46218">
        <property type="entry name" value="LASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46218:SF2">
        <property type="entry name" value="LIM AND SH3 DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00880">
        <property type="entry name" value="Nebulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00227">
        <property type="entry name" value="NEBU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51216">
        <property type="entry name" value="NEBULIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="15" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 18" id="KW-0440">LIM domain</keyword>
    <keyword evidence="9 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 19" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="11 18" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="22">
        <location>
            <begin position="3"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="21">
        <location>
            <begin position="174"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="129"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="142"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00025477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_017312834.1"/>
        </source>
    </evidence>
    <sequence length="233" mass="26569" checksum="2252F29C7CE82568" modified="2017-12-20" version="1">MNPLCSRCNRVVYPTEKVNCLDKFWHKGCFSCEVCKMTLNMKNYKGFDKKPYCNAHYPKTSFTSVADTPENLRLKQQSKMQSQVLYKEEFEKSKGKGFSVVADTPEMQRIKKTQDQISNIKYHEDFEKSKMGGEVHHPPPQQNTTPAYQPPAASQNYHYEPEPVRQAAAAPPPSSGKRYRAVYDYAAADEDEVSFLDGDVIVDVQSIDEGWMYGRVERTGQQGMLPANYVEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q6L0</accession>
    <name>A0A2D0Q6L0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Gamma-crystallin M2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC108258879</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017313341.2">
        <property type="nucleotide sequence ID" value="XM_017457852.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q6L0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000036242"/>
    <dbReference type="GeneID" id="108258879"/>
    <dbReference type="KEGG" id="ipu:108258879"/>
    <dbReference type="OMA" id="MGRSHEC"/>
    <dbReference type="OrthoDB" id="8407241at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000001">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000003">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF62">
        <property type="entry name" value="CRYGM2B PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="28"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="67"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="159"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017313341.2"/>
        </source>
    </evidence>
    <sequence length="203" mass="24442" checksum="6A23CA155AD38221" modified="2023-02-22" version="2">MAEVKPMSSVSSPPGKCWDTHTRIVNMGRVIFYEDRNFQGRSYECSGDCSDMSTYLSRCHSCRVESGCWMAYDRPNYMGNQYFMRRGEYADYMNMWGWGNNCIRSCRMIPMHRGAYRMRLYERDNFMGQMNEVMDDCDSVSDRYHWSSNGCMSCHVMDGHWLMYEHPHYKGRMWYFGPGEYRNFRDYGGMRFMSMRRIMDSWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q918</accession>
    <name>A0A2D0Q918_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">TOX high mobility group box family member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tox3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017314789.1">
        <property type="nucleotide sequence ID" value="XM_017459300.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q918"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000024161"/>
    <dbReference type="GeneID" id="108259654"/>
    <dbReference type="KEGG" id="ipu:108259654"/>
    <dbReference type="CTD" id="27324"/>
    <dbReference type="OMA" id="AYSKFGN"/>
    <dbReference type="OrthoDB" id="10027956at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21995">
        <property type="entry name" value="HMG-box_TOX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="256"/>
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Ictalurus punctatus</name>
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        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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            <taxon>Ostariophysi</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
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    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
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        <text evidence="10">Together with the RING-H2 protein ANAPC11, constitutes the catalytic component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 drives presynaptic differentiation.</text>
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        <text evidence="1">Protein modification; protein ubiquitination.</text>
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    <comment type="subunit">
        <text evidence="11">The mammalian APC/C is composed at least of 14 distinct subunits ANAPC1, ANAPC2, CDC27/APC3, ANAPC4, ANAPC5, CDC16/APC6, ANAPC7, CDC23/APC8, ANAPC10, ANAPC11, CDC26/APC12, ANAPC13, ANAPC15 and ANAPC16 that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa; APC/C interacts with FZR1 and FBXO5. In the context of the APC/C complex, directly interacts with UBE2C and UBE2S. Interacts (via cullin domain) with ANAPC11 and with UBCH10. Interacts with NEUROD2. Interacts with FBXO43; the interaction is direct.</text>
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        <text evidence="13">Belongs to the cullin family.</text>
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    </dbReference>
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        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 2"/>
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    </dbReference>
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    </dbReference>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cullin family profile" evidence="15">
        <location>
            <begin position="482"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="436"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="701"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="447"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
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    </evidence>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017315473.1"/>
        </source>
    </evidence>
    <sequence length="805" mass="91421" checksum="433AEB3A2AD86E75" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QC55</accession>
    <name>A0A2D0QC55_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">RuvB-like helicase</fullName>
            <ecNumber evidence="15">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ruvbl2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Proposed core component of the chromatin remodeling Ino80 complex which exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Dynein axonemal particle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the RuvB family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="15"/>
    <dbReference type="RefSeq" id="XP_017316263.1">
        <property type="nucleotide sequence ID" value="XM_017460774.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QC55"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000014544"/>
    <dbReference type="GeneID" id="108260457"/>
    <dbReference type="KEGG" id="ipu:108260457"/>
    <dbReference type="CTD" id="10856"/>
    <dbReference type="OMA" id="IINTEPY"/>
    <dbReference type="OrthoDB" id="10060499at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120293">
        <property type="term" value="C:dynein axonemal particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070286">
        <property type="term" value="P:axonemal dynein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003351">
        <property type="term" value="P:epithelial cilium movement involved in extracellular fluid movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060420">
        <property type="term" value="P:regulation of heart growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002221">
        <property type="entry name" value="RuvB-like 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000010">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.360:FF:000002">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.360">
        <property type="entry name" value="RuvB-like helicase, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027238">
        <property type="entry name" value="RuvB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041048">
        <property type="entry name" value="RuvB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042487">
        <property type="entry name" value="RuvBL1/2_DNA/RNA_bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010339">
        <property type="entry name" value="TIP49_P-loop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11093">
        <property type="entry name" value="RUVB-RELATED REPTIN AND PONTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06068">
        <property type="entry name" value="TIP49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17856">
        <property type="entry name" value="TIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 15" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="11 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 15" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="69"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017316263.1"/>
        </source>
    </evidence>
    <sequence length="463" mass="51277" checksum="6A448318151CE0F8" modified="2017-12-20" version="1">MAAQMTTTKVPEVRDLTRIERIGAHSHIRGLGLDDALEPRQVSQGMVGQLAARRAAGLILEMIKDGQIAGRAVLIAGQPGTGKTAIAMGIAQSLGQDTPFTALAGSEIFSLEMSKTEALSQAFRKAIGVRIKEETEIIEGEVVEIQIDRPATGTGAKVGKLTLKTTEMETIYDLGNKMIESLTKEKVQAGDVITIDKATGKICKLGRSFTRARDYDAMGAQTQFVQCPEGELQKRKEVVHTVSLHEIDVINSRTQGFLALFSGDTGEIKSEIREQINAKVSEWREEGKAEIIPGVLFIDEVHMLDIECFSFLNRALESDLSPVLIMATNRGITRIRGTNYQSPHGIPIDLLDRLLIIATSPYTEKETRQILKIRCEEEDVELSEEAHTVLTRIGQETSLRYAIQLISTAGLVCRKRRGTEVQVEDIKRVYSLFLDESRSSQYMKECQDSFLFNEAQPSQMDMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QEF2</accession>
    <name>A0A2D0QEF2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Krueppel-like factor 3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">klf3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017316669.1">
        <property type="nucleotide sequence ID" value="XM_017461180.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QEF2"/>
    <dbReference type="GeneID" id="108260714"/>
    <dbReference type="KEGG" id="ipu:108260714"/>
    <dbReference type="CTD" id="51274"/>
    <dbReference type="OMA" id="RIPHEYE"/>
    <dbReference type="OrthoDB" id="4748970at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21577">
        <property type="entry name" value="KLF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000021">
        <property type="entry name" value="Basic krueppel-like factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000018">
        <property type="entry name" value="Krueppel-like factor 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF120">
        <property type="entry name" value="KRUPPEL-LIKE FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="253"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="283"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="313"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="93"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="179"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="194"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017316669.1"/>
        </source>
    </evidence>
    <sequence length="338" mass="37265" checksum="2A151BD25197DEC0" modified="2017-12-20" version="1">MDVYNLTTFTIPVGAAMLMYDYPVKADRETGCPSLVKSQAKTYSAMFSIGPHPSHYCCAPHYVPYHSPTHTHTHTPPHGPDAPTHAQLEPVDLSLSKKSSPPPPSSSSSPSSSSPSPYSSSRATPPSPYERIGRSDSASMMLSFPSLAPGPGLIPVLSSMILPSCTMLYPPHVLLFPSTNDDQKGGASSLKSDGAQDHQPIKSEPHSKRAPSMHRQEIPSPPMPSYNEVNTPSVIVHAPKAESPDPLKKRRIHRCDFGGCNKVYTKSSHLKAHRRTHTGEKPYKCSWDGCTWKFARSDELTRHYRKHTGSKPFKCSDCDRSFSRSDHLALHRKRHLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QES7</accession>
    <name>A0A2D0QES7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Derlin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">derl2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functional component of endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by forming a channel that allows the retrotranslocation of misfolded proteins into the cytosol where they are ubiquitinated and degraded by the proteasome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the derlin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017316231.1">
        <property type="nucleotide sequence ID" value="XM_017460742.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QES7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034056"/>
    <dbReference type="GeneID" id="108260437"/>
    <dbReference type="KEGG" id="ipu:108260437"/>
    <dbReference type="CTD" id="51009"/>
    <dbReference type="OMA" id="FKSQYWR"/>
    <dbReference type="OrthoDB" id="1716531at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000016">
        <property type="entry name" value="Derlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007599">
        <property type="entry name" value="DER1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11009">
        <property type="entry name" value="DER1-LIKE PROTEIN, DERLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04511">
        <property type="entry name" value="DER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="153"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="229"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017316231.1"/>
        </source>
    </evidence>
    <sequence length="239" mass="27695" checksum="616EB13AA4A04939" modified="2017-12-20" version="1">MAYQTFQQEYLQIPVVTRTYTTACVLTTAAVQLELITPFQLYFNPDLILKQYQVWRLVTNFLFFGPVGFNFLFNMIFLYRYCRMLEEGSFRGRTADFVFMFLFGGLLMTIFGMFVSLVFLGQAFTIMLVYVWSRRNPNVRMNFFGLLNFQAPFLPWVLMGFSLLLGNSIIVDLLGIAVGHVYFFLEDVFPNQPGGSRWLRTPSFLKMLFDTPEEDPNYNPLPEDRPGGFPWGEGQRLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QG54</accession>
    <name>A0A2D0QG54_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Clathrin heavy chain linker domain-containing protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC108260732</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017316707.1">
        <property type="nucleotide sequence ID" value="XM_017461218.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QG54"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022517"/>
    <dbReference type="GeneID" id="108260732"/>
    <dbReference type="KEGG" id="ipu:108260732"/>
    <dbReference type="OMA" id="IGTMNKF"/>
    <dbReference type="OrthoDB" id="2113814at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012331">
        <property type="entry name" value="Clathrin_H-chain_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017212">
        <property type="entry name" value="CLHC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032755">
        <property type="entry name" value="TSNAXIP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10292:SF11">
        <property type="entry name" value="CLATHRIN HEAVY CHAIN LINKER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10292">
        <property type="entry name" value="CLATHRIN HEAVY CHAIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13838">
        <property type="entry name" value="Clathrin_H_link"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15739">
        <property type="entry name" value="TSNAXIP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037469">
        <property type="entry name" value="Clathrin_H-chain-rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translin-associated factor X-interacting protein 1 N-terminal" evidence="2">
        <location>
            <begin position="25"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017316707.1"/>
        </source>
    </evidence>
    <sequence length="618" mass="69620" checksum="F100378A067C4354" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QK79</accession>
    <name>A0A2D0QK79_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Endonuclease domain-containing 1 protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC108261698</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017318000.1">
        <property type="nucleotide sequence ID" value="XM_017462511.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QK79"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000015995"/>
    <dbReference type="GeneID" id="108261698"/>
    <dbReference type="KEGG" id="ipu:108261698"/>
    <dbReference type="OMA" id="FREKCAD"/>
    <dbReference type="OrthoDB" id="69221at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.570.10">
        <property type="entry name" value="Extracellular Endonuclease, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044929">
        <property type="entry name" value="DNA/RNA_non-sp_Endonuclease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001604">
        <property type="entry name" value="Endo_G_ENPP1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039015">
        <property type="entry name" value="ENDOD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020821">
        <property type="entry name" value="ENPP1-3/EXOG-like_nuc-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044925">
        <property type="entry name" value="His-Me_finger_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21472">
        <property type="entry name" value="ENDONUCLEASE DOMAIN-CONTAINING 1 PROTEIN ENDOD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21472:SF30">
        <property type="entry name" value="ENDONUCLEASE DOMAIN-CONTAINING 1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01223">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00892">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00477">
        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54060">
        <property type="entry name" value="His-Me finger endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013379424" evidence="1">
        <location>
            <begin position="19"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/RNA non-specific endonuclease/pyrophosphatase/phosphodiesterase" evidence="3">
        <location>
            <begin position="59"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="ENPP1-3/EXOG-like endonuclease/phosphodiesterase" evidence="2">
        <location>
            <begin position="60"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017318000.1"/>
        </source>
    </evidence>
    <sequence length="272" mass="30250" checksum="232BC05C42241A5F" modified="2017-12-20" version="1">MKLLSVVLLLLPLSLCLARVVNDFTKSCPGFFATPNDVVSPPTGFTGDRFKRICQTLNNKAEFATLYDTTYRIPVYSAYRFTGRVDCNRTTRNNWYIEPQLEDGNLGPNMATESAVRNIVNQAVNADYAGSPYDRGHLAPVFHAASQSCSDATFTLTNAAPQNLSFNRGQWKVTERKMAVSLNESCRNVGLSAFVVTGVVPGNYKMNNRVQIPSHFWTAFCCLDNNNIPRRSGGYIGENINNVVSGPMTNRDLERQLYNLYGRTVTLFGGRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QMG6</accession>
    <name>A0A2D0QMG6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6 7">Mitochondrial transcription rescue factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6 7" type="primary">mtres1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5 6 7" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5 6 7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017319592.1">
        <property type="nucleotide sequence ID" value="XM_017464103.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017319593.1">
        <property type="nucleotide sequence ID" value="XM_017464104.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053535276.1">
        <property type="nucleotide sequence ID" value="XM_053679301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QMG6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003129"/>
    <dbReference type="GeneID" id="108263382"/>
    <dbReference type="KEGG" id="ipu:108263382"/>
    <dbReference type="CTD" id="51250"/>
    <dbReference type="OMA" id="SLFCSCQ"/>
    <dbReference type="OrthoDB" id="4150at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903108">
        <property type="term" value="P:regulation of mitochondrial transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057896">
        <property type="entry name" value="MTRES1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13633">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION RESCUE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13633:SF3">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION RESCUE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25818">
        <property type="entry name" value="MTRES1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Mitochondrial transcription rescue factor 1 C-terminal" evidence="3">
        <location>
            <begin position="121"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="62"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017319592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017319593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053535276.1"/>
        </source>
    </evidence>
    <sequence length="229" mass="26113" checksum="476A45D1973D8E01" modified="2017-12-20" version="1">MQSLSVQALTLRQLGRLTSLQVPCAWHGASLLLRCPRALHARQLWGPSAPLVQRSAALLKHRGAARGPPYQQTRFKGYKKKKGRAGSAQEEEDEEEEEEDPEASDYEDELQEDPSLPKDYKDHQKAVQSLRFDLVMKTGLDVGRHKIEDAFYDHKLRLNGQKLIKKSKTVKVGDTLDLIVEEDKEKDEVTLKRVVLKKIVGETNDGEKQKVLLRSWKHLQLPKRDAFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QQA3</accession>
    <name>A0A2D0QQA3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Flap endonuclease 1</fullName>
            <shortName evidence="17">FEN-1</shortName>
            <ecNumber evidence="17">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Flap structure-specific endonuclease 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">fen1</name>
        <name evidence="21" type="synonym">fen1b</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14 17">Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="17">Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with PCNA. Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity. The C-terminal domain binds EP300; can bind simultaneously to both PCNA and EP300. Interacts with DDX11; this interaction is direct and increases flap endonuclease activity of FEN1. Interacts with WDR4; regulating its endonuclease activity. Interacts with POLB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Nucleus</location>
            <location evidence="17">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Nucleus</location>
            <location evidence="17">Nucleoplasm</location>
        </subcellularLocation>
        <text evidence="17">Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15 17">Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="17"/>
    <dbReference type="RefSeq" id="XP_017320548.1">
        <property type="nucleotide sequence ID" value="XM_017465059.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QQA3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000001864"/>
    <dbReference type="GeneID" id="100528612"/>
    <dbReference type="CTD" id="2237"/>
    <dbReference type="OMA" id="MGIPWVQ"/>
    <dbReference type="OrthoDB" id="1937206at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09867">
        <property type="entry name" value="PIN_FEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000009">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000003">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00614">
        <property type="entry name" value="Fen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023426">
        <property type="entry name" value="Flap_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF51">
        <property type="entry name" value="FLAP ENDONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00842">
        <property type="entry name" value="XPG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 17" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 17" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 17" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6 17 21" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9 17" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 17" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 17" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="19">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="18">
        <location>
            <begin position="146"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017320548.1"/>
        </source>
    </evidence>
    <sequence length="380" mass="42573" checksum="CE70FF2DB86DAC67" modified="2017-12-20" version="1">MGIHGLAKLIADQAPTAIREQDIKNYFGRKIAIDASMCIYQFLIAVRQDGNVLQNDDGETTSHLMGMFYRTIRMLDSGIKPVYVFDGKPPQLKSGELEKRGERRAEAEKLLAQAQETGEQENIDKFSKRLVKVTKQHNDECKKLLTLMGVPYIEAPCEAEASCAALVKAGKVYATATEDMDGLTFGTSVLLRHLTASEAKKLPIQEFHFSRVLQEMSLSHQQFIDLCILLGCDYCGTIKGIGPKRAIDLIKQHGSIEEILDNIDQSKHPAPEDWLYKEARALFLEPEVVDCTAMELKWNEPDEDGLVQFMCSEKQFSEDRIRNGCKKIMKSRQGSTQGRLDTFFTVTGSISSKRKEPEVKGSAKKKQKTSATPGKFKKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QW13</accession>
    <name>A0A2D0QW13_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Exosome complex component 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">exosc10</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">SDA103</strain>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="21">Component of the RNA exosome complex. The catalytically inactive RNA exosome core complex (Exo-9) associates with the catalytic subunit EXOSC10/RRP6 (via its N-terminus). Exo-9 may associate with DIS3 to form the nucleolar exosome complex, or DIS3L to form the cytoplasmic exosome complex. The RNA exosome complex interacts with cofactors C1D/RRP47, MPHOSPH6/MPP6 and MTREX/MTR4. Interacts with MTREX; the interaction with MTREX mediates the association of MTREX with nuclear RNA exosomes. Part of the small subunit (SSU) processome, composed of more than 70 proteins and the RNA chaperone small nucleolar RNA (snoRNA) U3. Interacts with ALYREF/THOC4. Interacts with DHX36; this interaction occurs in a RNase-insensitive manner. Interacts with NRDE2. Interacts (via C-terminus) with USP36 (via C-terminus); the interaction is facilitated by the association with RNA and promotes sumoylation of EXOSC10.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the exosome component 10/RRP6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017322612.1">
        <property type="nucleotide sequence ID" value="XM_017467123.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QW13"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032681"/>
    <dbReference type="GeneID" id="108265041"/>
    <dbReference type="KEGG" id="ipu:108265041"/>
    <dbReference type="CTD" id="5394"/>
    <dbReference type="OMA" id="NIMRPQM"/>
    <dbReference type="OrthoDB" id="2250022at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071044">
        <property type="term" value="P:histone mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071040">
        <property type="term" value="P:nuclear polyadenylation-dependent antisense transcript catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071039">
        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071036">
        <property type="term" value="P:nuclear polyadenylation-dependent snoRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071037">
        <property type="term" value="P:nuclear polyadenylation-dependent snRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019219">
        <property type="term" value="P:regulation of nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06147">
        <property type="entry name" value="Rrp6p_like_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000022">
        <property type="entry name" value="Exosome component 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.80:FF:000001">
        <property type="entry name" value="Putative exosome component 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="entry name" value="HRDC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012588">
        <property type="entry name" value="Exosome-assoc_fac_Rrp6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002121">
        <property type="entry name" value="HRDC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045092">
        <property type="entry name" value="Rrp6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049559">
        <property type="entry name" value="Rrp6p-like_exo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12124:SF47">
        <property type="entry name" value="EXOSOME COMPONENT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12124">
        <property type="entry name" value="POLYMYOSITIS/SCLERODERMA AUTOANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00570">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08066">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="14" id="KW-0269">Exonuclease</keyword>
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    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="7" id="KW-0698">rRNA processing</keyword>
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        </location>
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        <location>
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            <end position="632"/>
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        <location>
            <begin position="725"/>
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        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
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            <begin position="14"/>
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        </location>
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            <end position="840"/>
        </location>
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        </source>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00004604"/>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022499"/>
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        </source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00043957"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00065628"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00070703"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50967"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017322612.1"/>
        </source>
    </evidence>
    <sequence length="900" mass="102267" checksum="3C7F0172FCB524EA" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
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    <name>A0A2D0QW51_ICTPU</name>
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        <name type="scientific">Ictalurus punctatus</name>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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        </subcellularLocation>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    </dbReference>
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    </dbReference>
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    <proteinExistence type="predicted"/>
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        </location>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="381" mass="41372" checksum="C08900B5E794B04F" modified="2017-12-20" version="1">MTAEVQQPSVQPPAQSSPMSAPEKAHAQSAAMDAASSTTKAKKTNAGIRRPEKPPYSYIALIVMAIQSSPTKRLTLSEIYQFLQSRFPFFRGSYQGWKNSVRHNLSLNECFIKLPKGLGRPGKGHYWTIDPASEFMFEEGSFRRRPRGFRRKCQALKPSMYSMMNGLGFNHLPESYNFQGSGGGLSCPPNSLSLESGIGMMNGHLAGNMEGMGLAGHSMSHLSANSGHSYMGNCTGSTGSEYPHHDNSASPLLSGGGVMEPHPVYSSTTSAWAPAPSASLNNGASYIKQQPLSPCNPGANPLQPSLPTHSLDQSYLHQNGHSTTDLQGIPRYHSQSPSMCDRKEFVFSFNAMTSSTMHSPGSGSYYHHQQVAYQDIKPCVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R2N9</accession>
    <name>A0A2D0R2N9_ICTPU</name>
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        <submittedName>
            <fullName evidence="10">Helix-loop-helix protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">nhlh2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017324839.1">
        <property type="nucleotide sequence ID" value="XM_017469350.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R2N9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000017886"/>
    <dbReference type="GeneID" id="108266261"/>
    <dbReference type="KEGG" id="ipu:108266261"/>
    <dbReference type="CTD" id="4808"/>
    <dbReference type="OrthoDB" id="10067827at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19701">
        <property type="entry name" value="bHLH_TS_HEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000027">
        <property type="entry name" value="Nescient helix-loop-helix 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040238">
        <property type="entry name" value="TAL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13864:SF10">
        <property type="entry name" value="HELIX-LOOP-HELIX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13864">
        <property type="entry name" value="T-CELL ACUTE LYMPHOCYTIC LEUKEMIA/STEM CELL LEUKEMIA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="2" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="66"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017324839.1"/>
        </source>
    </evidence>
    <sequence length="124" mass="14309" checksum="76207A868886A103" modified="2017-12-20" version="1">MKMMLSPDQTDPDLMWAQSDPETVLNVIKVECMPKESPLADGKQRALAPPALTREEKRRRRRATAKYRLAHATRERIRVEAFNVAFAELRKLLPTLPPDKKLSKIEILRLAICYISYLNHVLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R9S6</accession>
    <name>A0A2D0R9S6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Interleukin-27 subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ebi3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type I cytokine receptor family. Type 3 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017326585.1">
        <property type="nucleotide sequence ID" value="XM_017471096.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R9S6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012504"/>
    <dbReference type="GeneID" id="108267125"/>
    <dbReference type="KEGG" id="ipu:108267125"/>
    <dbReference type="CTD" id="10148"/>
    <dbReference type="OMA" id="FQVCAKE"/>
    <dbReference type="OrthoDB" id="6381660at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056621">
        <property type="entry name" value="FN3_IL27B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003530">
        <property type="entry name" value="Hematopoietin_rcpt_L_F3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053073">
        <property type="entry name" value="IL11/IL27_subunit_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48483">
        <property type="entry name" value="INTERLEUKIN-27 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48483:SF2">
        <property type="entry name" value="INTERLEUKIN-27 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24031">
        <property type="entry name" value="FN3_IL27B_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01354">
        <property type="entry name" value="HEMATOPO_REC_L_F3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012722848" evidence="6">
        <location>
            <begin position="22"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="7">
        <location>
            <begin position="197"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017326585.1"/>
        </source>
    </evidence>
    <sequence length="294" mass="33044" checksum="30F4A64F232D62B3" modified="2017-12-20" version="1">MCLKSSLYALSLFAFFPQFWSQDTSSEPKDLYAALGSSVELPCVNGRTNGSEWRLNGSFLISSPVLRLHNTSLADQGFYTCHDSNGEIVETVRLQLGYPPSLPDVHCWSPSYPLKALCSWTLPQDPILPTYYISTYRYLEDIYSCQRQSEQDRQCVLEELDLFSIVPYLVNITAVNALGSRSRVLPVILEDIVKPDPPVNLKVTALPGKKLHLQWAPPPTWPDPVTFPLKYKVQFQWGHNDATSIMGPTESESMVRSGVMPGRTYRIRVSVMDLLGHGQSSDWSDTVNITLPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R9T2</accession>
    <name>A0A2D0R9T2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">Zona pellucida sperm-binding protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17 18" type="primary">LOC108267598</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17 18">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Zona pellucida</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Cell membrane</location>
            <topology evidence="14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.</text>
    </comment>
    <comment type="PTM">
        <text evidence="14">Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the ZP domain family. ZPC subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017327328.2">
        <property type="nucleotide sequence ID" value="XM_017471839.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047012789.1">
        <property type="nucleotide sequence ID" value="XM_047156833.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R9T2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000529"/>
    <dbReference type="GeneID" id="108267598"/>
    <dbReference type="KEGG" id="ipu:108267598"/>
    <dbReference type="OrthoDB" id="8880842at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035805">
        <property type="term" value="C:egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032190">
        <property type="term" value="F:acrosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035804">
        <property type="term" value="F:structural constituent of egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035803">
        <property type="term" value="P:egg coat formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000344">
        <property type="term" value="P:positive regulation of acrosome reaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.3210:FF:000001">
        <property type="entry name" value="Zona pellucida sperm-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.4100:FF:000002">
        <property type="entry name" value="Zona pellucida sperm-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055356">
        <property type="entry name" value="ZP-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576:SF2">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23344">
        <property type="entry name" value="ZP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 14" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="12 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 14" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 14" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044514881" description="Zona pellucida sperm-binding protein 3" evidence="14">
        <location>
            <begin position="17"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="15">
        <location>
            <begin position="71"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017327328.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_047012789.1"/>
        </source>
    </evidence>
    <sequence length="399" mass="44702" checksum="5FB832252195BB28" modified="2023-02-22" version="2">MIQSVLLISLAVHAASVPRASYEDLNSDYFSRQKTADIQFSNQESPKIFPGRLFSNLENVSESESGGLVVKCEENNWRIVVKRQYFGPGLRLSSRSVRLGEDGAPGECTPLKDFITPTEMVISAGLQDCGSESRVSDNWLIYSNKLVFSHETHLTWNGVHVLKHPPTIVPIECRYKRKFRVSAEPLSPTWLPMTSTIEAVGLLHFSLDVIKGDVGSLHQSTVYQQGEPLLLEAAVHSPMHRPLRIYVDLCEASVDSDTLSWPSYEFISDHGCLIDSMFPYSSSKFYSRPKQNILRFSIQAFFFPQYQRKQVFISCHLRAAPIYSLPDSQNKACYFHGPSLSWHAVEGSSNVCLCCETATCKSGMKDPSSKYLDEQTRVVGPLQILPANVHWTGKLTIGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R9X0</accession>
    <name>A0A2D0R9X0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GON-4-like protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">GON-4 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">gon4lb</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Has transcriptional repressor activity, probably as part of a complex with YY1, SIN3A and HDAC1. Required for B cell lymphopoiesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Found in a complex with YY1, SIN3A and HDAC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017327368.1">
        <property type="nucleotide sequence ID" value="XM_017471879.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000006356"/>
    <dbReference type="GeneID" id="108267623"/>
    <dbReference type="KEGG" id="ipu:108267623"/>
    <dbReference type="CTD" id="555764"/>
    <dbReference type="OrthoDB" id="6257037at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12202">
        <property type="entry name" value="CASP8AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000191">
        <property type="entry name" value="GON-4-like protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000006">
        <property type="entry name" value="GON-4-like protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.11">
        <property type="entry name" value="Paired amphipathic helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049257">
        <property type="entry name" value="Gon4l/CASP8AP2_myb-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003822">
        <property type="entry name" value="PAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036600">
        <property type="entry name" value="PAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052435">
        <property type="entry name" value="YY1-Transcr_Regul"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16088:SF3">
        <property type="entry name" value="GON-4-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16088">
        <property type="entry name" value="YY1 ASSOCIATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21227">
        <property type="entry name" value="Myb_DNA-binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02671">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47762">
        <property type="entry name" value="PAH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51477">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="51"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="244"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="328"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="445"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="497"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1106"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1171"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1390"/>
            <end position="1702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1920"/>
            <end position="1943"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1974"/>
            <end position="2134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2201"/>
            <end position="2224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="253"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="497"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1108"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1182"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1416"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1493"/>
            <end position="1512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1526"/>
            <end position="1552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1553"/>
            <end position="1568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1594"/>
            <end position="1614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1630"/>
            <end position="1651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1676"/>
            <end position="1694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1974"/>
            <end position="1983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="2006"/>
            <end position="2034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="2063"/>
            <end position="2082"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="2095"/>
            <end position="2108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="2121"/>
            <end position="2132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="2208"/>
            <end position="2224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017327368.1"/>
        </source>
    </evidence>
    <sequence length="2224" mass="246350" checksum="CCD878663C1028C8" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RB73</accession>
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        <recommendedName>
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        <name type="scientific">Ictalurus punctatus</name>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
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                <person name="Gao S."/>
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                <person name="Sun F."/>
                <person name="Li C."/>
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                <person name="Yuan Z."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="33">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="34">Blood</tissue>
        </source>
    </reference>
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        <subcellularLocation>
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            <location evidence="1">Cilium</location>
        </subcellularLocation>
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        <subcellularLocation>
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            <location evidence="7">Extracellular exosome</location>
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            <location evidence="5">Extracellular space</location>
            <location evidence="5">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="29">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017472310.3"/>
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        <property type="component" value="Chromosome 7"/>
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        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Olfactomedin-like_domain"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="26" id="KW-0966">Cell projection</keyword>
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            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="Olfactomedin-like" evidence="32">
        <location>
            <begin position="175"/>
            <end position="435"/>
        </location>
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    <feature type="coiled-coil region" evidence="30">
        <location>
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        </location>
    </feature>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="SAM" id="Coils"/>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RCD9</accession>
    <name>A0A2D0RCD9_ICTPU</name>
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        <submittedName>
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    <gene>
        <name evidence="5" type="primary">apol1</name>
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    <organism evidence="4 5">
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        <name type="common">Channel catfish</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the apolipoprotein L family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017327550.1">
        <property type="nucleotide sequence ID" value="XM_017472061.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RCD9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020355"/>
    <dbReference type="GeneID" id="108267717"/>
    <dbReference type="KEGG" id="ipu:108267717"/>
    <dbReference type="CTD" id="8542"/>
    <dbReference type="OrthoDB" id="6363454at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042157">
        <property type="term" value="P:lipoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008405">
        <property type="entry name" value="ApoL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14096">
        <property type="entry name" value="APOLIPOPROTEIN L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14096:SF59">
        <property type="entry name" value="APOLIPOPROTEIN L, 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05461">
        <property type="entry name" value="ApoL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="412"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="491"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="491"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="511"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017327550.1"/>
        </source>
    </evidence>
    <sequence length="532" mass="57720" checksum="009DFD75EFC4CE2E" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RDV7</accession>
    <name>A0A2D0RDV7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Ventral anterior homeobox 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">vax2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMX homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017328075.1">
        <property type="nucleotide sequence ID" value="XM_017472586.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RDV7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000011588"/>
    <dbReference type="GeneID" id="108267973"/>
    <dbReference type="KEGG" id="ipu:108267973"/>
    <dbReference type="CTD" id="25806"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061386">
        <property type="term" value="P:closure of optic fissure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902254">
        <property type="term" value="P:negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021554">
        <property type="term" value="P:optic nerve development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090259">
        <property type="term" value="P:regulation of retinal ganglion cell axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060042">
        <property type="term" value="P:retina morphogenesis in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000375">
        <property type="entry name" value="Ventral anterior homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050877">
        <property type="entry name" value="EMX-VAX-Noto_Homeobox_TFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24339">
        <property type="entry name" value="HOMEOBOX PROTEIN EMX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24339:SF34">
        <property type="entry name" value="VENTRAL ANTERIOR HOMEOBOX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="98"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="100"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="194"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="284"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="13"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="46"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="156"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="202"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="291"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017328075.1"/>
        </source>
    </evidence>
    <sequence length="307" mass="32680" checksum="18F109D495B445D0" modified="2017-12-20" version="1">MFDQAKSMGDGVAEDRNHCGPNTLRRERGGDAKTLAEVGSRSSGDAPAGTSASTPSSSSEDGHDKLLGVDPDYCRRILVRDAKGTIREIVLPKGLDLDRPKRTRTSFTAEQLYRLELEFQRCQYVVGRERTELARQLNLSETQVKVWFQNRRTKQKKDQTKDSDKRSSSTSESLATCNILRLLEQGRLLSVPAPPPNPLLAPPHAANGSLLGGPSVSSSSPGVSSTGTPPAIVASGGGTFGLSLSSLGATPPSPRLGVAPPSLFSMPLMSGAHHELTSSYGCGSSAFEPYTRTERKDGDLGGKKTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RG67</accession>
    <name>A0A2D0RG67_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transmembrane protein 216</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">tmem216</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Part of the tectonic-like complex which is required for tissue-specific ciliogenesis and may regulate ciliary membrane composition.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017329529.1">
        <property type="nucleotide sequence ID" value="XM_017474040.3"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_047157206.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RG67"/>
    <dbReference type="GeneID" id="108268819"/>
    <dbReference type="KEGG" id="ipu:108268819"/>
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    <dbReference type="GeneTree" id="ENSGT00940000153899"/>
    <dbReference type="OrthoDB" id="262535at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:convergent extension involved in gastrulation"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:photoreceptor cell outer segment organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019184">
        <property type="entry name" value="Uncharacterised_TM-17"/>
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        <property type="entry name" value="GEO07735P1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR13531:SF5">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09799">
        <property type="entry name" value="Transmemb_17"/>
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    </dbReference>
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    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
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        <location>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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            <end position="151"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037712"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00039543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017329529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_047013162.1"/>
        </source>
    </evidence>
    <sequence length="157" mass="17999" checksum="17CEB6D5E53A8720" modified="2017-12-20" version="1">MSLTFRVFQQKPILILPFLTKSQLSSMPLQVLFYLNSWYFAAFFIAEILMFIYKGVLLPYPQANLILDIVLLLVYLGLEALRLFYGWKGNLCERSLMLIVSVGVLVPCTVLSVYYMLLQTFILRLEFVLSAILLCFYALELVLALITIAAFSRASVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RL51</accession>
    <name>A0A2D0RL51_ICTPU</name>
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        <submittedName>
            <fullName evidence="12">Interleukin 2 receptor, gamma b isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">il2rgb</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
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        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017330675.1">
        <property type="nucleotide sequence ID" value="XM_017475186.3"/>
    </dbReference>
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    <dbReference type="STRING" id="7998.ENSIPUP00000032372"/>
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    <dbReference type="KEGG" id="ipu:108269358"/>
    <dbReference type="CTD" id="100144554"/>
    <dbReference type="OrthoDB" id="8942047at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016064">
        <property type="term" value="P:immunoglobulin mediated immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CRLF2-like_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
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        <property type="entry name" value="FN3_sf"/>
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        <property type="entry name" value="Hempt_rcpt_S_F1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037:SF42">
        <property type="entry name" value="CYTOKINE RECEPTOR COMMON SUBUNIT GAMMA ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21604">
        <property type="entry name" value="CRLF2_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fibronectin type III"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HEMATOPO_REC_S_F1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012926215" evidence="9">
        <location>
            <begin position="20"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="234"/>
            <end position="257"/>
        </location>
    </feature>
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        <location>
            <begin position="129"/>
            <end position="225"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017330675.1"/>
        </source>
    </evidence>
    <sequence length="334" mass="37772" checksum="E2B6186018A71EB1" modified="2017-12-20" version="1">MTYLLTAAVILFFFQGCWSRAQFSPSVGCTSTDYEVNVSCLIINLDYVECNWTKPQMQHINYTFSSAFSWDSFSECPEYLQEQGQSVGCKIPLDATQRFQPFRTKLYVGGYQTICKNYTDLKQRVKLNPPSILSVNSSAREGEVCVRWIGSKIKKNCVNYTVAYRKASGPWKISIPVMSSSYCVSPVASGVLYTFQVRSSMSDMCGASDFWSDWSDPVQWSNNKDVMTAEQPQVYWHVLGSVLGVIVLICLSLLLYYSERIRVVFVPVVPDPSKSLQDLFKKYNGNVESWVYINRELKDAFETDFTESPPCVVCEAGPTFETKTEDGPVEQPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RM38</accession>
    <name>A0A2D0RM38_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rhotekin-2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108269931</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017331577.1">
        <property type="nucleotide sequence ID" value="XM_017476088.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RM38"/>
    <dbReference type="GeneID" id="108269931"/>
    <dbReference type="KEGG" id="ipu:108269931"/>
    <dbReference type="OrthoDB" id="5817051at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030097">
        <property type="term" value="P:hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13249">
        <property type="entry name" value="PH_rhotekin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051364">
        <property type="entry name" value="Cytokinesis/Rho-signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21538">
        <property type="entry name" value="ANILLIN/RHOTEKIN RTKN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21538:SF21">
        <property type="entry name" value="RHOTEKIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="1">
        <location>
            <begin position="27"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017331577.1"/>
        </source>
    </evidence>
    <sequence length="222" mass="24967" checksum="2FD3A6284126FA67" modified="2017-12-20" version="1">MEASSWLPLYGNLCCQLVAQPDCMTQGMMSGYLSQQQSVEGLQHCCRLYCVLKAGLLSCYFTPEEIQAKIEPSVIISINKDTRLRVVECNQKAGARLSIIDPGTGNPATHVFIAETPDLLQEWLSVLLQHIYDQSQWQHTCDKLMEIEVLSPPKPPLFLTKQADSVYNDLSIGSLWTSFNLAALESFASRRRFSHQLCSLGQSHRSNHKGHEKPYSHCINLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RWH2</accession>
    <name>A0A2D0RWH2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Alpha/beta hydrolase domain-containing protein 17C</fullName>
            <ecNumber evidence="3">3.1.2.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">abhd17c</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>S-hexadecanoyl-L-cysteinyl-[protein] + H2O = L-cysteinyl-[protein] + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="3.1.2.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Postsynaptic density membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the AB hydrolase superfamily. ABHD17 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017334300.1">
        <property type="nucleotide sequence ID" value="XM_017478811.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RWH2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034572"/>
    <dbReference type="GeneID" id="108271310"/>
    <dbReference type="KEGG" id="ipu:108271310"/>
    <dbReference type="CTD" id="58489"/>
    <dbReference type="OMA" id="TGQASEC"/>
    <dbReference type="OrthoDB" id="446723at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099175">
        <property type="term" value="P:regulation of postsynapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000008">
        <property type="entry name" value="Alpha/beta hydrolase domain-containing protein 17B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF55">
        <property type="entry name" value="ALPHA_BETA HYDROLASE DOMAIN-CONTAINING PROTEIN 17C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="6 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="17">
        <location>
            <begin position="97"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017334300.1"/>
        </source>
    </evidence>
    <sequence length="294" mass="32687" checksum="B11AADC25A79CC3C" modified="2017-12-20" version="1">MPEQGPRMNGFSLGELCWLFCCPPCPSRIAAKLAFLPPEPTYSVHTDTEGACSLHLSERADWQYSQRELDAVEVFGTRTSRGNRVLCMFVRCAPSSRYTLLFSHGNAVDLGQMCSFYIGLGSRINCNVFSYDYSGYGVSTGKPSEKNLYADIEAAWQVLRTKYGVTPENIILYGQSIGTVPTVDLAARYECAAVILHSPLMSGLRVAFPNTRKTYCFDAFPSIDKVSKVASPVLVIHGTEDEVIDFSHGLAIYERCPRAVEPLWVEGAGHNDIELYAQYLERLKQFISFELATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RYB4</accession>
    <name>A0A2D0RYB4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="17">RNA-binding protein 5 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">rbm5</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017335493.1">
        <property type="nucleotide sequence ID" value="XM_017480004.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RYB4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000897"/>
    <dbReference type="GeneID" id="108271979"/>
    <dbReference type="KEGG" id="ipu:108271979"/>
    <dbReference type="CTD" id="10181"/>
    <dbReference type="OMA" id="MYDDRSP"/>
    <dbReference type="OrthoDB" id="29221at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12755">
        <property type="entry name" value="RRM2_RBM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000110">
        <property type="entry name" value="RNA-binding protein 10 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1060.10:FF:000005">
        <property type="entry name" value="RNA-binding protein 10 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041591">
        <property type="entry name" value="OCRE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034993">
        <property type="entry name" value="RBM5_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
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        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA-BINDING PROTEIN 5"/>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
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    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="6 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="106"/>
            <end position="186"/>
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        <location>
            <begin position="763"/>
            <end position="809"/>
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            <begin position="1"/>
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    <feature type="region of interest" description="Disordered" evidence="11">
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            <begin position="694"/>
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        <location>
            <begin position="1"/>
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        <location>
            <begin position="536"/>
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        <location>
            <begin position="645"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
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        <source>
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        <source>
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    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50174"/>
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        <source>
            <dbReference type="PROSITE" id="PS50199"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017335493.1"/>
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    <sequence length="835" mass="93199" checksum="82D0C8D29B9B707E" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RZH2</accession>
    <name>A0A2D0RZH2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Membrane-associated guanylate kinase inverted 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC108272162</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MAGUK family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="PDZ0_MAGI-1_3-like"/>
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        <property type="entry name" value="PDZ1_MAGI-1_3-like"/>
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        <property type="entry name" value="PDZ2_MAGI-1_3-like"/>
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        <property type="entry name" value="PDZ3_MAGI-1_3-like"/>
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        <property type="entry name" value="PDZ5_MAGI-1_3-like"/>
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    </dbReference>
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        <property type="entry name" value="Guanylate Kinase phosphate binding domain"/>
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    </dbReference>
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        <property type="entry name" value="GK/Ca_channel_bsu"/>
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        <property type="entry name" value="Guanylate_kin-like_dom"/>
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        <property type="entry name" value="Guanylate_kinase_CS"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
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        <property type="entry name" value="PDZ_sf"/>
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    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
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    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
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    <dbReference type="PANTHER" id="PTHR10316">
        <property type="entry name" value="MEMBRANE ASSOCIATED GUANYLATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10316:SF65">
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    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GuKc"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0796">Tight junction</keyword>
    <keyword evidence="18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PDZ" evidence="16">
        <location>
            <begin position="65"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="15">
        <location>
            <begin position="111"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="297"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="343"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="16">
        <location>
            <begin position="422"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="16">
        <location>
            <begin position="596"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="16">
        <location>
            <begin position="749"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="16">
        <location>
            <begin position="874"/>
            <end position="961"/>
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
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                <person name="Duke M.V."/>
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                <person name="Kucuktas H."/>
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                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
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</entry>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
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    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="small_GTP"/>
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    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017338797.1"/>
        </source>
    </evidence>
    <sequence length="229" mass="25931" checksum="106A778AD65C35A7" modified="2017-12-20" version="1">MSSAMESRVARRCKIVVVGDSRCGKTALLHVFAKDSYPESYVPTVFENYTASFEIDKRRIELNMWDTSGSTYYDNVRPLAYPDSDAVLICFDISRPETLDNVLKKWQGETQEYCPNAKVVLVGCKLDMRTDLNTLRELSKLRLIPITHEQGTSLARQIGAAAYAECTSKNSEDSVRDVFHIATVTCVGYDTQRLKRTGSRRGLKRASQNPQRSEILELRKDRAKSCTLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S8L2</accession>
    <name>A0A2D0S8L2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">taok2b</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017339077.1">
        <property type="nucleotide sequence ID" value="XM_017483588.3"/>
    </dbReference>
    <dbReference type="GeneID" id="108273910"/>
    <dbReference type="KEGG" id="ipu:108273910"/>
    <dbReference type="CTD" id="100151551"/>
    <dbReference type="OrthoDB" id="10016527at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000030">
        <property type="entry name" value="Serine/threonine-protein kinase TAO2, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000029">
        <property type="entry name" value="Serine/threonine-protein kinase TAO2, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051234">
        <property type="entry name" value="TAO_STE20_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47167">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TAO1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47167:SF6">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE TAO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1011"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1043"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1074"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="28"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="346"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="566"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="720"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1243"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="468"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="347"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="423"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="734"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="765"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="804"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="846"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="893"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1243"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1259"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017339077.1"/>
        </source>
    </evidence>
    <sequence length="1291" mass="147491" checksum="8D542CFF812B6E75" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S8U1</accession>
    <name>A0A2D0S8U1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tectonin beta-propeller repeat-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">tecpr1a</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Autophagosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TECPR1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017339163.1">
        <property type="nucleotide sequence ID" value="XM_017483674.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S8U1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000004066"/>
    <dbReference type="GeneID" id="108273961"/>
    <dbReference type="KEGG" id="ipu:108273961"/>
    <dbReference type="CTD" id="556500"/>
    <dbReference type="OrthoDB" id="72441at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000690">
        <property type="entry name" value="Tectonin beta-propeller repeat-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006624">
        <property type="entry name" value="Beta-propeller_rpt_TECPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006614">
        <property type="entry name" value="Peroxin/Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010482">
        <property type="entry name" value="TECPR1-like_DysF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051513">
        <property type="entry name" value="Tectonin_beta-prop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23250">
        <property type="entry name" value="DYSFERLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23250:SF11">
        <property type="entry name" value="TECTONIN BETA-PROPELLER REPEAT-CONTAINING PROTEIN 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06462">
        <property type="entry name" value="Hyd_WA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06398">
        <property type="entry name" value="Pex24p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19193">
        <property type="entry name" value="Tectonin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00694">
        <property type="entry name" value="DysFC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00693">
        <property type="entry name" value="DysFN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00706">
        <property type="entry name" value="TECPR"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0458">Lysosome</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Peroxin/Ferlin" evidence="12">
        <location>
            <begin position="64"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Peroxin/Ferlin" evidence="13">
        <location>
            <begin position="137"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Peroxin/Ferlin" evidence="12">
        <location>
            <begin position="836"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="domain" description="Peroxin/Ferlin" evidence="13">
        <location>
            <begin position="908"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="409"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="460"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1172"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="462"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017339163.1"/>
        </source>
    </evidence>
    <sequence length="1194" mass="133423" checksum="0CBF6E7B23DA2FAE" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SB24</accession>
    <name>A0A2D0SB24_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Coiled-coil domain-containing protein 186</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ccdc186</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017339430.1">
        <property type="nucleotide sequence ID" value="XM_017483941.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SB24"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000025384"/>
    <dbReference type="GeneID" id="108274085"/>
    <dbReference type="KEGG" id="ipu:108274085"/>
    <dbReference type="CTD" id="55088"/>
    <dbReference type="OMA" id="TLSNGMC"/>
    <dbReference type="OrthoDB" id="5583482at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099518">
        <property type="term" value="P:vesicle cytoskeletal trafficking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038830">
        <property type="entry name" value="CCDC186"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18911:SF5">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18911">
        <property type="entry name" value="CTCL TUMOR ANTIGEN HD-CL-01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="69"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="774"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="839"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="174"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="774"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="789"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017339430.1"/>
        </source>
    </evidence>
    <sequence length="972" mass="110671" checksum="34096D514600C1F7" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SEW7</accession>
    <name>A0A2D0SEW7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Electron transfer flavoprotein beta subunit lysine methyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ETFB lysine methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Protein N-lysine methyltransferase METTL20</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">etfbkmt</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54192"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13826"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:54193"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methyltransferase superfamily. ETFBKMT family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017341267.1">
        <property type="nucleotide sequence ID" value="XM_017485778.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SEW7"/>
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        <property type="entry name" value="Vaccinia Virus protein VP39"/>
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    <keyword evidence="1 9" id="KW-0489">Methyltransferase</keyword>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037932"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00040322"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00041867"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00042266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017341267.1"/>
        </source>
    </evidence>
    <sequence length="256" mass="29439" checksum="D41A000BA5630D99" modified="2017-12-20" version="1">MNRGLFICLRGKDALRKPHQFVRYGSIRQYMKSKSENEIKRFIEENTEIVKGQSLTPEISLRLFTVKCRFWTERPEFWPFPDPFWAIYWPGGQALTRYLLNNPELSVGRRVLDLGCGCGASAIAAKLSGAAHVVANDIDPIAAIATKMNCELNGLEPLPCVTENLISSEPENWDLILLGDMFYDETLTDSLHKWLYKCVKLYRTQIFIGDPGRAQFESCDIKTLLYEQARFELPDSVREENYGLTSSTVWCYRPEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SMB3</accession>
    <name>A0A2D0SMB3_ICTPU</name>
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        <submittedName>
            <fullName evidence="8">Granulin a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">grna</name>
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    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the granulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017343844.2">
        <property type="nucleotide sequence ID" value="XM_017488355.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SMB3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000014593"/>
    <dbReference type="GeneID" id="108276572"/>
    <dbReference type="KEGG" id="ipu:108276572"/>
    <dbReference type="CTD" id="791524"/>
    <dbReference type="OrthoDB" id="5854875at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.160:FF:000001">
        <property type="entry name" value="Granulin precursor"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.160">
        <property type="entry name" value="Granulin"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000118">
        <property type="entry name" value="Granulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039036">
        <property type="entry name" value="Granulin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037277">
        <property type="entry name" value="Granulin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12274">
        <property type="entry name" value="GRANULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12274:SF8">
        <property type="entry name" value="GRANULIN-A ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00396">
        <property type="entry name" value="Granulin"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00277">
        <property type="entry name" value="GRAN"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57277">
        <property type="entry name" value="Granulin repeat"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00799">
        <property type="entry name" value="GRANULINS"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039885784" evidence="5">
        <location>
            <begin position="17"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="domain" description="Granulins" evidence="6">
        <location>
            <begin position="50"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Granulins" evidence="6">
        <location>
            <begin position="132"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Granulins" evidence="6">
        <location>
            <begin position="215"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Granulins" evidence="6">
        <location>
            <begin position="299"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Granulins" evidence="6">
        <location>
            <begin position="383"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Granulins" evidence="6">
        <location>
            <begin position="467"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Granulins" evidence="6">
        <location>
            <begin position="551"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="Granulins" evidence="6">
        <location>
            <begin position="635"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="Granulins" evidence="6">
        <location>
            <begin position="717"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="domain" description="Granulins" evidence="6">
        <location>
            <begin position="801"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="domain" description="Granulins" evidence="6">
        <location>
            <begin position="885"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="domain" description="Granulins" evidence="6">
        <location>
            <begin position="1050"/>
            <end position="1063"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017343844.2"/>
        </source>
    </evidence>
    <sequence length="1090" mass="117738" checksum="3D21FF14A168C563" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SV82</accession>
    <name>A0A2D0SV82_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coiled-coil domain-containing protein 150 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ccdc150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017346431.1">
        <property type="nucleotide sequence ID" value="XM_017490942.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SV82"/>
    <dbReference type="GeneID" id="108277901"/>
    <dbReference type="KEGG" id="ipu:108277901"/>
    <dbReference type="CTD" id="284992"/>
    <dbReference type="OrthoDB" id="416454at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038807">
        <property type="entry name" value="CCDC150"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35352">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35352:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="92"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="498"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="610"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017346431.1"/>
        </source>
    </evidence>
    <sequence length="775" mass="89361" checksum="6D9B402898023CFE" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SWH1</accession>
    <name>A0A2D0SWH1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Trafficking protein particle complex subunit 6B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 11 12" type="primary">trappc6bl</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11 12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11 12">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of a transport protein particle (TRAPP) complex that may function in specific stages of inter-organelle traffic. Specifically involved in the early development of neural circuitry, likely by controlling the frequency and amplitude of intracellular calcium transients implicated in the regulation of neuron differentiation and survival.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347032.1">
        <property type="nucleotide sequence ID" value="XM_017491543.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017347033.1">
        <property type="nucleotide sequence ID" value="XM_017491544.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017347034.1">
        <property type="nucleotide sequence ID" value="XM_017491545.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SWH1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026962"/>
    <dbReference type="GeneID" id="108278287"/>
    <dbReference type="KEGG" id="ipu:108278287"/>
    <dbReference type="CTD" id="393634"/>
    <dbReference type="GeneTree" id="ENSGT00390000012948"/>
    <dbReference type="OMA" id="PACELHY"/>
    <dbReference type="OrthoDB" id="941624at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14944">
        <property type="entry name" value="TRAPPC6A_Trs33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1380.20:FF:000004">
        <property type="entry name" value="Trafficking protein particle complex subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037992">
        <property type="entry name" value="TRAPPC6/Trs33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12817:SF7">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX 6B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12817">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00074542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017347032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017347033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017347034.1"/>
        </source>
    </evidence>
    <sequence length="161" mass="18503" checksum="CBB7645E982C70F4" modified="2017-12-20" version="1">MADDILFEFLHMEMVSHIYTEHTTREDIERERVTCVSVLEGMGFRVGQGLIERFTKDCPTFKDDLDIMKFLCKDFWNGIFRKQIDNLRTNHQGTYVLQDNKFALLTRVSGRTQQLEESSKYLSYTCGLIRGALSNLGVETVVTAEVSSMPSCKFQVVVQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SY10</accession>
    <name>A0A2D0SY10_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Gamma-aminobutyric acid receptor subunit alpha-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">GABA(A) receptor subunit alpha-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">gabra1</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">SDA103</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="27">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="21">Postsynaptic cell membrane</location>
            <topology evidence="21">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347411.1">
        <property type="nucleotide sequence ID" value="XM_017491922.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SY10"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000024634"/>
    <dbReference type="GeneID" id="108278498"/>
    <dbReference type="KEGG" id="ipu:108278498"/>
    <dbReference type="CTD" id="2554"/>
    <dbReference type="OMA" id="YLWAYLF"/>
    <dbReference type="OrthoDB" id="203862at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004890">
        <property type="term" value="F:GABA-A receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19034">
        <property type="entry name" value="LGIC_ECD_GABAAR_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19052">
        <property type="entry name" value="LGIC_TM_GABAAR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000001">
        <property type="entry name" value="Gamma-aminobutyric acid A receptor subunit alpha-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000002">
        <property type="entry name" value="Putative gamma-aminobutyric acid receptor subunit alpha-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001390">
        <property type="entry name" value="GABAAa_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005431">
        <property type="entry name" value="GABBAa1_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047024">
        <property type="entry name" value="Gabra-1-6_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047079">
        <property type="entry name" value="GABRA1_ECD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01079">
        <property type="entry name" value="GABAARALPHA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01614">
        <property type="entry name" value="GABAARALPHA1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0868">Chloride</keyword>
    <keyword evidence="13" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="18 22" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9 22" id="KW-0406">Ion transport</keyword>
    <keyword evidence="17" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="10 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="12 27" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 22" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 22" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022256733" description="Gamma-aminobutyric acid receptor subunit alpha-1" evidence="22">
        <location>
            <begin position="23"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="248"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="313"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="24">
        <location>
            <begin position="41"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="25">
        <location>
            <begin position="255"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="385"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="397"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00033008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_017347411.1"/>
        </source>
    </evidence>
    <sequence length="456" mass="51384" checksum="B3281DB05500DB4D" modified="2017-12-20" version="1">MMWARLGAAWIWAWLLAVNVLGKSSNQNGSDEQKDNTTVFTRILDSLLDGYDNRLRPGLGERVTEVKTDIFVTSIGPVSDHDMEYTIDVFFRQSWKDERLKFKGPMAVLRLNNLMASKIWTPDTFFHNGKKSVAHNMTMPNKLLRITEEGTLLYTMRLTVRAECPMHLEDFPMDAHACPLKFGSYAYTRAEVVYVWTRGAAQSVVVAEDGSRLNQYDLMGQTVDSGVVQSSTGEYVVMTTHFHLKRKIGYFVIQTYLPCIMTVILSQVSFWLNRESVPARTVFGVTTVLTMTTLSISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKRGYAWDGKSVVPEKQKKKKESLLKKNNTYTAATATAFAPNIARDPGLATIAKSAPPPPTEPKEEPKPKPAEAKKTFNSVSKIDRIARIAFPLLFGTFNLVYWATYLNKKPKIQDALTPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SYC3</accession>
    <name>A0A2D0SYC3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Glutamine synthetase</fullName>
            <ecNumber evidence="3 11">6.3.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC108278589</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-glutamate + NH4(+) + ATP = L-glutamine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="3 11"/>
    <dbReference type="RefSeq" id="XP_017347526.1">
        <property type="nucleotide sequence ID" value="XM_017492037.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SYC3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000015072"/>
    <dbReference type="GeneID" id="108278589"/>
    <dbReference type="KEGG" id="ipu:108278589"/>
    <dbReference type="OMA" id="NAKYMAL"/>
    <dbReference type="OrthoDB" id="1936100at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.70:FF:000004">
        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000011">
        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027302">
        <property type="entry name" value="Gln_synth_N_conserv_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050292">
        <property type="entry name" value="Glutamine_Synthetase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852:SF43">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00180">
        <property type="entry name" value="GLNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017347526.1"/>
        </source>
    </evidence>
    <sequence length="380" mass="42400" checksum="71400E056CCBC4D5" modified="2017-12-20" version="1">MSSSLSVSSQLNKVLRSHYLSLPQGGLCLVTYVWIDGSGESLRSKTRTLESEPSSIEDIPEWNFDGSSTNQAETSSSDMILVPVCIFRDPFTLDPNKLVLCEVLKHNCKPADTNHRHVCKKIMEKVKDFHPWFGMEQEYTLLGIDGHPYAWPSKGYPKPQGPYYCGVGADRAYGRDIVECHYKACIYAGIKICGANAEVKPSQWEFQVGPCEGIEVGDHLWMARFLLHRVCEDFGVIATLDPKPIPGNWNGAGCHTNFSTEATRAEGGLEHIEKIIEKLRAHHVQHIRVSDPHGGQDNQRRLTGFNETSSIHEFSAAVADRGVSIRIPLQVGKDKRGYLEDRRPAANCDPYAVTSAIAQTSLMEEEEEDTLNDLDINDLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SYU9</accession>
    <name>A0A2D0SYU9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Guanine nucleotide-binding protein subunit alpha-13b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">gna13b</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-alpha family. G(12) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347701.2">
        <property type="nucleotide sequence ID" value="XM_017492212.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SYU9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000001520"/>
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        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
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        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT ALPHA-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
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    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00440">
        <property type="entry name" value="GPROTEINA12"/>
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        <property type="entry name" value="G_alpha"/>
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        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
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        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="58"/>
            <end position="63"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
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        <location>
            <position position="62"/>
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        <ligand>
            <name>Mg(2+)</name>
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            <begin position="219"/>
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        <ligand>
            <name>GTP</name>
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        <location>
            <begin position="288"/>
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        <ligand>
            <name>GTP</name>
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        <source>
            <dbReference type="RefSeq" id="XP_017347701.2"/>
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    <sequence length="374" mass="43557" checksum="E0933DD4E48D0587" modified="2023-02-22" version="2">MADFLPSRSFLSICFPKCLLNTSEAEQLRISKEIDRCISKDKTYVKRLVKILLLGAGESGKSTFLKQMRIIHGQDFDQLAREEFRATIYSNVIKGIRVLVDAREKLHIPWGDASNQAHGEAVMSFDTRSTQGMVEPKVFQQYIPAVRALWADSGIQHAYDRRREFQLGESVKYFLDNLEKLGMPDYLPSQQDILLARRPTKGIHEYDFEIKNVPFKMVDVGGQRSERRRWFECFDSVTSILFLVSSSEFDQVLMEDRLTNRLTESLNIFGTIINNRVFSNVSIILFLNKTDLLEEKVKIVSIKDFFPEYEGDPHSLDDVQKFLVTCFRNQRREQQNKPLYHHFTTAINTENIRLVFRDVKDTILHDNLKQLMLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T4W9</accession>
    <name>A0A2D0T4W9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Uridylate-specific endoribonuclease</fullName>
            <ecNumber evidence="11">4.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">si:dkey-222f8.3</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ribonucleotidyl-uridine-RNA = a 5'-end dephospho-uridine-RNA + a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA</text>
            <dbReference type="Rhea" id="RHEA:67792"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:17354"/>
            <dbReference type="Rhea" id="RHEA-COMP:17356"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:173117"/>
            <dbReference type="ChEBI" id="CHEBI:173224"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 11">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ENDOU family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_017349655.1">
        <property type="nucleotide sequence ID" value="XM_017494166.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T4W9"/>
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    <dbReference type="KEGG" id="ipu:108279736"/>
    <dbReference type="OrthoDB" id="430326at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21159">
        <property type="entry name" value="XendoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039787">
        <property type="entry name" value="ENDOU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037227">
        <property type="entry name" value="EndoU-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018998">
        <property type="entry name" value="EndoU_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12439">
        <property type="entry name" value="PLACENTAL PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12439:SF33">
        <property type="entry name" value="URIDYLATE-SPECIFIC ENDORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09412">
        <property type="entry name" value="XendoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142877">
        <property type="entry name" value="EndoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51959">
        <property type="entry name" value="ENDOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="EndoU" evidence="12">
        <location>
            <begin position="7"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017349655.1"/>
        </source>
    </evidence>
    <sequence length="289" mass="32828" checksum="542CBFE1BA5C88B9" modified="2017-12-20" version="1">MAKRPAVDQELSAILNELWKLDVNRVKPGKDYTINLQGKADYVAQGSNSARDKANVPLFTYVNEDKLKSIATYASFINLLDNYEMSTGVAEKVTSEELEENNIFLNAILQTQVMKRAHGYLVSKGQASSDVAQFKKLLFDIWFKLYHRDRSGGEDSCGFEHVFVGETKYGKEIMGLHNWVQFYLQEKLNFIDYKGYKARDKDMPDANDHVINLQFSWKGLVKPLGSSFIGVSPEFEVALFTIIFLTSNERVTNAIVKVDEYLLEIVVCRHGRAIGTSYPKLLSSNNRDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T6I4</accession>
    <name>A0A2D0T6I4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein FAM227A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108280194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM227 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017350507.3">
        <property type="nucleotide sequence ID" value="XM_017495018.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T6I4"/>
    <dbReference type="GeneID" id="108280194"/>
    <dbReference type="KEGG" id="ipu:108280194"/>
    <dbReference type="OrthoDB" id="192208at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029417">
        <property type="entry name" value="FAM227"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33560:SF1">
        <property type="entry name" value="PROTEIN FAM227A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33560">
        <property type="entry name" value="PROTEIN FAM227B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14922">
        <property type="entry name" value="FWWh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017350507.3"/>
        </source>
    </evidence>
    <sequence length="360" mass="40842" checksum="2AFEE090F38030E4" modified="2023-06-28" version="3">MATERIRGLEPIGTASKMEDTPKMVELYQCPQFRDVPVPLPYNTAYRAIITRVIQAQRHLVRKPSLKAVFHNILSSPNVEHFVISSFWWLFLENFQPDRKAQDRLFGQIAENYISILTQSLSSNSGTIFLQDFPSTLSQMLFCCFSCCFPQSCCFRDPAFLTALCSTVYQWTGGLSPAPEIYKQWDFEALEENETSNIIDREKKEKDSDSSLTSTDSVFSSIFLSEPSGSLTAMKNRMKSPFEVSQAKALYSKNKLTTTNHASTCEVEKAASQNVADSDLKVLTDQRNHTLARESHAVDGQIKVKHCVFNVWGNSPLVQHYMNTLHLQCNVGYNILVRRTQLQALPQYPHTCAIYQDAHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T7J0</accession>
    <name>A0A2D0T7J0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rho GTPase-activating protein 29</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Rho-type GTPase-activating protein 29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC108280415</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017350844.1">
        <property type="nucleotide sequence ID" value="XM_017495355.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T7J0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000024079"/>
    <dbReference type="GeneID" id="108280415"/>
    <dbReference type="KEGG" id="ipu:108280415"/>
    <dbReference type="OrthoDB" id="79452at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20816">
        <property type="entry name" value="C1_GMIP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054713">
        <property type="entry name" value="GMIP/FCHO2-like_FCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057028">
        <property type="entry name" value="RHG29_45_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051025">
        <property type="entry name" value="RhoGAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15228:SF7">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15228">
        <property type="entry name" value="SPERMATHECAL PHYSIOLOGY VARIANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22699">
        <property type="entry name" value="GMIP-like_FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24235">
        <property type="entry name" value="RHG29_45_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="F-BAR" evidence="12">
        <location>
            <begin position="176"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="10">
        <location>
            <begin position="605"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="11">
        <location>
            <begin position="666"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="319"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="463"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1048"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1174"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="476"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="526"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="577"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1060"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1083"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1097"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1174"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1210"/>
            <end position="1224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017350844.1"/>
        </source>
    </evidence>
    <sequence length="1224" mass="136186" checksum="ED9D89917A1C730E" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T9P8</accession>
    <name>A0A2D0T9P8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">von Willebrand factor A domain-containing protein 5B1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">vwa5b2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017351603.1">
        <property type="nucleotide sequence ID" value="XM_017496114.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000013696"/>
    <dbReference type="GeneID" id="108280745"/>
    <dbReference type="KEGG" id="ipu:108280745"/>
    <dbReference type="CTD" id="90113"/>
    <dbReference type="OMA" id="QPPRCHV"/>
    <dbReference type="OrthoDB" id="1729737at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013694">
        <property type="entry name" value="VIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052627">
        <property type="entry name" value="VWA_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46299:SF2">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46299">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5B2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13757">
        <property type="entry name" value="VIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13768">
        <property type="entry name" value="VWA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51468">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="366"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="962"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1135"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1021"/>
            <end position="1033"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017351603.1"/>
        </source>
    </evidence>
    <sequence length="1300" mass="143650" checksum="9249E1FA74CE9045" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979E4B1</accession>
    <name>A0A979E4B1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Protein mono-ADP-ribosyltransferase PARP12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC124626777</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047008698.2">
        <property type="nucleotide sequence ID" value="XM_047152742.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979E4B1"/>
    <dbReference type="GeneID" id="124626777"/>
    <dbReference type="KEGG" id="ipu:124626777"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990404">
        <property type="term" value="F:NAD+-protein mono-ADP-ribosyltransferase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01439">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051712">
        <property type="entry name" value="ARTD-AVP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056226">
        <property type="entry name" value="WH_PARP12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004170">
        <property type="entry name" value="WWE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037197">
        <property type="entry name" value="WWE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057602">
        <property type="entry name" value="Zfn-CCCH_PARP12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45740">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45740:SF6">
        <property type="entry name" value="PROTEIN MONO-ADP-RIBOSYLTRANSFERASE PARP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24356">
        <property type="entry name" value="WHD_PARP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02825">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23466">
        <property type="entry name" value="WWE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25261">
        <property type="entry name" value="zf-CCCH_PARP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117839">
        <property type="entry name" value="WWE domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50918">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="WWE" evidence="13">
        <location>
            <begin position="354"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="PARP catalytic" evidence="14">
        <location>
            <begin position="467"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_047008698.2"/>
        </source>
    </evidence>
    <sequence length="669" mass="76215" checksum="645D27D6F3720C33" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F010</accession>
    <name>A0A979F010_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Prominin-1-A isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">prom1a</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Microvillus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prominin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047013279.1">
        <property type="nucleotide sequence ID" value="XM_047157323.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979F010"/>
    <dbReference type="GeneID" id="108269184"/>
    <dbReference type="CTD" id="322857"/>
    <dbReference type="OrthoDB" id="6229420at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031528">
        <property type="term" value="C:microvillus membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071914">
        <property type="term" value="C:prominosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008795">
        <property type="entry name" value="Prominin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22730">
        <property type="entry name" value="PROMININ PROM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22730:SF3">
        <property type="entry name" value="PROMININ-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05478">
        <property type="entry name" value="Prominin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5038054111" evidence="8">
        <location>
            <begin position="24"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="437"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="487"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="805"/>
            <end position="824"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_047013279.1"/>
        </source>
    </evidence>
    <sequence length="894" mass="99038" checksum="1146D0A12022A431" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F4C9</accession>
    <name>A0A979F4C9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein 239 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC108273009</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047014915.2">
        <property type="nucleotide sequence ID" value="XM_047158959.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979F4C9"/>
    <dbReference type="GeneID" id="108273009"/>
    <dbReference type="KEGG" id="ipu:108273009"/>
    <dbReference type="OrthoDB" id="427030at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000475">
        <property type="entry name" value="zinc finger protein 32 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001270">
        <property type="entry name" value="zinc finger protein 583 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002331">
        <property type="entry name" value="Zinc finger protein 672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050589">
        <property type="entry name" value="Ikaros_C2H2-ZF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404:SF111">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="137"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="193"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="221"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_047014915.2"/>
        </source>
    </evidence>
    <sequence length="279" mass="32071" checksum="6FAD3358A22600A9" modified="2023-06-28" version="2">MKSESRRRSLGKSPQTPAATGSKMYHCSDCGKSFTKTHHLKNHKCIHTEDKPHHCGQCGKSFTCQSHLKQHQRIHTGEKLYHCSQCGKSFTFQSNLQVHERIHTGEKPYHCSQCGKSFTSQSNLQVHKRIHTGEKRYYCSQCGNSFTRQSHLQRHELIHTGERPYHCSQCGKSFTRETHLQQHHRIHTGEKPYHCGQCGKSFNQEGNLRIHQRIHTGEKPYHCSQCGKCFHDPSAFHSHQQCHTGQKPYLCGHCGRSYAHARSLRIHKCSNIKPSALDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F8E1</accession>
    <name>A0A979F8E1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">Zona pellucida sperm-binding protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC124626046</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Zona pellucida</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Cell membrane</location>
            <topology evidence="14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.</text>
    </comment>
    <comment type="PTM">
        <text evidence="14">Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the ZP domain family. ZPC subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047016317.2">
        <property type="nucleotide sequence ID" value="XM_047160361.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979F8E1"/>
    <dbReference type="GeneID" id="124626046"/>
    <dbReference type="KEGG" id="ipu:124626046"/>
    <dbReference type="OrthoDB" id="8880842at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035805">
        <property type="term" value="C:egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032190">
        <property type="term" value="F:acrosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035804">
        <property type="term" value="F:structural constituent of egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035803">
        <property type="term" value="P:egg coat formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000344">
        <property type="term" value="P:positive regulation of acrosome reaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.3210:FF:000001">
        <property type="entry name" value="Zona pellucida sperm-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.4100:FF:000002">
        <property type="entry name" value="Zona pellucida sperm-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055356">
        <property type="entry name" value="ZP-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048290">
        <property type="entry name" value="ZP_chr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576:SF2">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23344">
        <property type="entry name" value="ZP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00023">
        <property type="entry name" value="ZPELLUCIDA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 14" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="12 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 14" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 14" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039963565" description="Zona pellucida sperm-binding protein 3" evidence="14">
        <location>
            <begin position="22"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="15">
        <location>
            <begin position="125"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_047016317.2"/>
        </source>
    </evidence>
    <sequence length="435" mass="47302" checksum="B59FAD7519040BD7" modified="2023-06-28" version="2">MGFNQVGFGVLLILAVGLSAAQWPGLRNVSAELQPNKPGHSLLQVSARLPSQWMQSGPQSGSPAVVPLGLHLQNPSDAQDQQVMQRPVKKLTWRFPAAPQIPTPPAPVNFMRQSNAVPVQSVTARCNETAVYVEVRKDLFGTRAPLNITALTLGGCAAKGVDVSSQVLVYESPLHGCNSKLTVTANELVYIFTLGIASVPLSSAPILRSPGAMVFIECHYMRFHNVSSGALLPAWIPYASAQAAEERLLFFLRLMTDDWIFERPSDYVFQLGELINIEASVVQFNHVPLRVFVDSCVATAVPDVNAVPRYSFIENHGCLIDAKLAHSSSRFMPQTQAAKLGFQLEAFKFQHVNSNWFYIACILKATAVSAPADAEHKACSFSGNRWTAAYGADQVCSCCSSSCGLRKGRDLSSEQGLQLEKEVSLGPIMVLENAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QT81</accession>
    <name>A0A9F7QT81_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ATP-binding cassette sub-family D member 3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC108280417</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053529644.1">
        <property type="nucleotide sequence ID" value="XM_053673669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QT81"/>
    <dbReference type="GeneID" id="108280417"/>
    <dbReference type="KEGG" id="ipu:108280417"/>
    <dbReference type="OrthoDB" id="422637at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000636">
        <property type="entry name" value="ATP-binding cassette sub-family D member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF62">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY D MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
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                <person name="Kucuktas H."/>
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                <person name="Liu H."/>
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                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
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                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="12">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="13">Blood</tissue>
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            <location evidence="1">Membrane</location>
        </subcellularLocation>
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        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    <proteinExistence type="inferred from homology"/>
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    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
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</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R2I3</accession>
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        <submittedName>
            <fullName evidence="6">Synaptotagmin-14 isoform X1</fullName>
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        <name evidence="6" type="primary">syt14a</name>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Liu S."/>
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                <person name="Bao L."/>
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                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
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                <person name="Gao S."/>
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                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
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                <person name="Yuan Z."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptotagmin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053535209.1">
        <property type="nucleotide sequence ID" value="XM_053679234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R2I3"/>
    <dbReference type="GeneID" id="108263192"/>
    <dbReference type="KEGG" id="ipu:108263192"/>
    <dbReference type="CTD" id="564702"/>
    <dbReference type="OrthoDB" id="5978493at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08408">
        <property type="entry name" value="C2B_Synaptotagmin-14_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000062">
        <property type="entry name" value="synaptotagmin-14 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043541">
        <property type="entry name" value="SYT14/14L/16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46129">
        <property type="entry name" value="SYNAPTOTAGMIN 14, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46129:SF3">
        <property type="entry name" value="SYNAPTOTAGMIN-14-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="4">
        <location>
            <begin position="388"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="157"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="178"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_053535209.1"/>
        </source>
    </evidence>
    <sequence length="528" mass="58499" checksum="DCD56B9D73F2A02F" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R2U2</accession>
    <name>A0A9F7R2U2_ICTPU</name>
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        <submittedName>
            <fullName evidence="10 11 12">NACHT, LRR and PYD domains-containing protein 3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 11 12" type="primary">LOC128630835</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11 12" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11 12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053535074.1">
        <property type="nucleotide sequence ID" value="XM_053679099.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053535075.1">
        <property type="nucleotide sequence ID" value="XM_053679100.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053535076.1">
        <property type="nucleotide sequence ID" value="XM_053679101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R2U2"/>
    <dbReference type="GeneID" id="128630835"/>
    <dbReference type="KEGG" id="ipu:128630835"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00116">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000714">
        <property type="entry name" value="Si:ch211-149a19.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000210">
        <property type="entry name" value="Si:dkey-16p6.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001632">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="8">
        <location>
            <begin position="230"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053535074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053535075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053535076.1"/>
        </source>
    </evidence>
    <sequence length="1039" mass="117894" checksum="C0B96CB1C239AA72" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R350</accession>
    <name>A0A9F7R350_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein LOC124626077</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC124626077</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053532492.1">
        <property type="nucleotide sequence ID" value="XM_053676517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R350"/>
    <dbReference type="GeneID" id="124626077"/>
    <dbReference type="KEGG" id="ipu:124626077"/>
    <dbReference type="OrthoDB" id="6780341at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053532492.1"/>
        </source>
    </evidence>
    <sequence length="201" mass="21960" checksum="61325663160585E5" modified="2023-06-28" version="1">MWYRNGQPVTDKLTKHIKLYLNCSEDAGNYSCAVRGREDLRSPELTVTCEPQNSGSVNYTVLKVIMVGLAVFLAVTLLSGALCMWKRKSSSANGHSSTGESGQRHSPAVYENVSASDRSGRVASDDQDDVHYASVVFKNSHTQEVSPSPRRRPDTTEYEDVQYAAVNVSRSTAAIQLVADEAADDPSQLYSKIQKLPTSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R3W6</accession>
    <name>A0A9F7R3W6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Calpain-5 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC108260142</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053532912.1">
        <property type="nucleotide sequence ID" value="XM_053676937.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R3W6"/>
    <dbReference type="GeneID" id="108260142"/>
    <dbReference type="OMA" id="SIHKTWH"/>
    <dbReference type="OrthoDB" id="424753at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04046">
        <property type="entry name" value="C2_Calpain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00214">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.380:FF:000003">
        <property type="entry name" value="Calpain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000027">
        <property type="entry name" value="Calpain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000173">
        <property type="entry name" value="Calpain 5b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033884">
        <property type="entry name" value="C2_Calpain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033883">
        <property type="entry name" value="C2_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022682">
        <property type="entry name" value="Calpain_domain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022683">
        <property type="entry name" value="Calpain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036213">
        <property type="entry name" value="Calpain_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF381">
        <property type="entry name" value="CALPAIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01067">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00704">
        <property type="entry name" value="CALPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00720">
        <property type="entry name" value="calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49758">
        <property type="entry name" value="Calpain large subunit, middle domain (domain III)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="8">
        <location>
            <begin position="24"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="7">
        <location>
            <begin position="498"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR622684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053532912.1"/>
        </source>
    </evidence>
    <sequence length="641" mass="73027" checksum="4B04B43822F5ADB6" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R582</accession>
    <name>A0A9F7R582_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">5-formyltetrahydrofolate cyclo-ligase</fullName>
            <ecNumber evidence="5 7">6.3.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">mthfs</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 7">
            <text>(6S)-5-formyl-5,6,7,8-tetrahydrofolate + ATP = (6R)-5,10-methenyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10488"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the 5-formyltetrahydrofolate cyclo-ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.2" evidence="5 7"/>
    <dbReference type="RefSeq" id="XP_053539000.1">
        <property type="nucleotide sequence ID" value="XM_053683025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R582"/>
    <dbReference type="GeneID" id="100528421"/>
    <dbReference type="CTD" id="10588"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030272">
        <property type="term" value="F:5-formyltetrahydrofolate cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009396">
        <property type="term" value="P:folic acid-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02727">
        <property type="entry name" value="MTHFS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407:SF1">
        <property type="entry name" value="5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407">
        <property type="entry name" value="ATPASE INHIBITOR/5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01812">
        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006806">
        <property type="entry name" value="FTHF_cligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="4"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="89"/>
            <end position="97"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00036539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006806-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_053539000.1"/>
        </source>
    </evidence>
    <sequence length="144" mass="16409" checksum="53CCE50573F7C7EC" modified="2023-06-28" version="1">MHDEVRTEAILQHMFSSGKVCFIPRYQSDSNHMDMLQLNSMEDLNSLPITSWNIRQPADNDTQREEALATGGLDLILMPGLGFDKNGNRLGRGKGFYDLYLQRCMRHSKGKPYTIALAFKEQLCEQIPVDDTDILIDEVLCEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R6B2</accession>
    <name>A0A9F7R6B2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA helicase</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">smarcad1a</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">DNA helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is both required for DNA repair and heterochromatin organization. Promotes DNA end resection of double-strand breaks (DSBs) following DNA damage: probably acts by weakening histone DNA interactions in nucleosomes flanking DSBs. Required for the restoration of heterochromatin organization after replication.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="RefSeq" id="XP_053536271.1">
        <property type="nucleotide sequence ID" value="XM_053680296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R6B2"/>
    <dbReference type="GeneID" id="108265971"/>
    <dbReference type="CTD" id="557875"/>
    <dbReference type="OrthoDB" id="448448at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14279">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17998">
        <property type="entry name" value="DEXHc_SMARCAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000014">
        <property type="entry name" value="SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000639">
        <property type="entry name" value="SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CUE" evidence="18">
        <location>
            <begin position="239"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="19">
        <location>
            <begin position="570"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="20">
        <location>
            <begin position="915"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="18"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="197"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="279"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="222"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="286"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="306"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="317"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="329"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="340"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="352"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="373"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="400"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="419"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_053536271.1"/>
        </source>
    </evidence>
    <sequence length="1082" mass="122814" checksum="17D0D8C19130783A" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R7W2</accession>
    <name>A0A9F7R7W2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">choline-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="11">2.7.7.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17 18" type="primary">LOC124628309</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17 18">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the key rate-limiting step in the CDP-choline pathway for phosphatidylcholine biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>phosphocholine + CTP + H(+) = CDP-choline + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18997"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
            <dbReference type="EC" id="2.7.7.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:18998"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.15" evidence="11"/>
    <dbReference type="RefSeq" id="XP_053537126.1">
        <property type="nucleotide sequence ID" value="XM_053681151.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053537127.1">
        <property type="nucleotide sequence ID" value="XM_053681152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R7W2"/>
    <dbReference type="GeneID" id="124628309"/>
    <dbReference type="KEGG" id="ipu:124628309"/>
    <dbReference type="OrthoDB" id="17102at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004105">
        <property type="term" value="F:choline-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000016">
        <property type="entry name" value="Putative choline-phosphate cytidylyltransferase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041723">
        <property type="entry name" value="CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045049">
        <property type="entry name" value="Pcy1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739:SF19">
        <property type="entry name" value="CHOLINE-PHOSPHATE CYTIDYLYLTRANSFERASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739">
        <property type="entry name" value="CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 17 18" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="15">
        <location>
            <begin position="78"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="237"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_053537126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_053537127.1"/>
        </source>
    </evidence>
    <sequence length="333" mass="38760" checksum="9BE3D5D09C8E1725" modified="2023-06-28" version="1">MDAHTSSHAPSRKRKRDDSNGETEEGETTARYKHHTVALRDPAPYVDQLHSLEDQPYERISMEEARRGTPPDRPVRVYADGIFDMFHSGHARALMQAKDLFPNTHLIVGVCSDDLTHKLKGFTVMNEEERYDAVTHCRYVDEVVRNAPWTLTPDFLTKHRIDFVAHDDIPYSSAGSDDVYKHIKEAGMFAPTQRTEGISTSDIITRIVRDYDVYVRRNLQRGYTAKELNVSFINEKKYHLQERVDKVKQKVRDVEEKSKEFVQKVEEQSIDLIQKWEEKSREFIGNFLQMFGPEGALVSRAAHKRKNLRILRRVNVHIRKSANVNVYMLFCRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R8H8</accession>
    <name>A0A9F7R8H8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein LOC128633015 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC128633015</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053537471.1">
        <property type="nucleotide sequence ID" value="XM_053681496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R8H8"/>
    <dbReference type="GeneID" id="128633015"/>
    <dbReference type="KEGG" id="ipu:128633015"/>
    <dbReference type="OrthoDB" id="6151406at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053537471.1"/>
        </source>
    </evidence>
    <sequence length="217" mass="23725" checksum="2BD1111267399757" modified="2023-06-28" version="1">MTISTVSKSDEGFYHCKHPKSGESPKSWISVRTSDSGSSGVIIGLSLAFLFVILMIVLILLLHFKKKKGVEYQTPSTVNQDTNQPADGDNIYENADIRTTEDSAAGKSSMIHSQDVHRNKTQNDALAGLSDVTYADIELKNQKKPKRKQGKSSEGADTVYSELKQNTDKDAAAGPSDVTYANIELKNQKKPKRKQGKSSEGADTVYSELKQNTDKGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R9V0</accession>
    <name>A0A9F7R9V0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tyrosine-protein phosphatase non-receptor type 20</fullName>
            <ecNumber evidence="1">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 12 13" type="primary">LOC108265758</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11 12 13" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11 12 13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Tyrosine-protein phosphatase targeted to sites of actin polymerization in response of varied extracellular stimuli. Has tyrosine phosphatase activity towards various tyrosyl phosphorylated substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="1"/>
    <dbReference type="RefSeq" id="XP_053536151.1">
        <property type="nucleotide sequence ID" value="XM_053680176.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053536152.1">
        <property type="nucleotide sequence ID" value="XM_053680177.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053536153.1">
        <property type="nucleotide sequence ID" value="XM_053680178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R9V0"/>
    <dbReference type="GeneID" id="108265758"/>
    <dbReference type="OrthoDB" id="8609993at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000102">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF557">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE ETA-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11 12 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="8">
        <location>
            <begin position="123"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="9">
        <location>
            <begin position="300"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="398"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="398"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="411"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="430"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00058215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00072470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053536151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053536152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_053536153.1"/>
        </source>
    </evidence>
    <sequence length="480" mass="55054" checksum="120122B7A092A67E" modified="2023-06-28" version="1">MTASRRFLYEEINRLQYSGVILAHSSKHIVFKSCSVGFQSGDGLGHSNALIFFSLQPIESFLCCMLWIVVLLEVHPHLIFIILVDGSRFFSGIFWIRTYPRAHILQKFHAHCQSLAANGNAGYQREFEELCDNAKEFSCRAGELDANKNKNRYPFILPYDHCRVKLSLLESQPHSDYINASYVPGGCTEHDFICTQAPLPSTMADFWRMVWEQNVQVIIMMTALKESDKVLCNQYWPPERGTGCYGALQVTTMSRHHGPYCYITTIHLRQHGSPTDRHITHYYYPGWPDQGVPKDHTSLSNFTEHVRKLLDDIPRLGPTVVHCSAGIGRSGTFVAILWLMQLCARGIPPDVHLAVRDLRRHRVLMVQNVEQYILVHQCLLHWLAGNTERPKTRNTVVHFQAAQPQTDRQSRRERKKQRRNSGHTHRPESTHSSLQVSHTPQPQHTAWQSLRDSLHAFNPGKLLQRIMPPSSQLTNSQTQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RB20</accession>
    <name>A0A9F7RB20_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Bifunctional endo-1,4-beta-xylanase XylA-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC128629171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053531923.1">
        <property type="nucleotide sequence ID" value="XM_053675948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RB20"/>
    <dbReference type="GeneID" id="128629171"/>
    <dbReference type="KEGG" id="ipu:128629171"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053531923.1"/>
        </source>
    </evidence>
    <sequence length="207" mass="25693" checksum="86026CFEAAC096C3" modified="2023-06-28" version="1">MARALNHMTASQVDAAHYSHEEQENRDRLSGEETQPIDLSDYPVDNQQTIQLTNQWTNQRTYQWTIQQTIQWTNQQTIQLTIQLTNQWTNQRTIQWSNQRTIHWTNQWTNQQTNQWTYQRTNQWNKQQTIQQAIQWTIQWTIQWTNQWTIQWTNQRTIQWTNQRTIQWSYQRTNQWNKQQTIQQAIQWTIQWTNQWTTQWTNQQTIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7REB8</accession>
    <name>A0A9F7REB8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="1">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC128630403</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="1"/>
    <dbReference type="RefSeq" id="XP_053534913.1">
        <property type="nucleotide sequence ID" value="XM_053678938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7REB8"/>
    <dbReference type="GeneID" id="128630403"/>
    <dbReference type="KEGG" id="ipu:128630403"/>
    <dbReference type="OrthoDB" id="26387at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF28">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053534913.1"/>
        </source>
    </evidence>
    <sequence length="133" mass="15189" checksum="4F550020C09CF3D6" modified="2023-06-28" version="1">MEMCILCHEVQEILPDGTAMVLAAFVQGSTVMSKNRKRPPHNPESCDPLFMHPDLSFGTRTGSCGHIMHSHCWQRYFETLQSQDAQWLHEQTSYDVENGEYLCPLCKCRSNTVIPLLPLTETTCRYSHTNLCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RIG5</accession>
    <name>A0A9F7RIG5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Hemicentin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Fibulin-6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="primary">hmcn1</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="29">SDA103</strain>
        </source>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="30">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Involved in transforming growth factor beta-mediated rearrangement of the podocyte cytoskeleton which includes reduction of F-actin fibers and broadening, flattening and elongation of podocytes. Plays a role in basement membrane organization. May promote cleavage furrow maturation during cytokinesis in preimplantation embryos. May play a role in the architecture of adhesive and flexible epithelial cell junctions. May play a role during myocardial remodeling by imparting an effect on cardiac fibroblast migration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
            <location evidence="4">Extracellular space</location>
            <location evidence="4">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="24">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053542916.1">
        <property type="nucleotide sequence ID" value="XM_053686941.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108280233"/>
    <dbReference type="KEGG" id="ipu:108280233"/>
    <dbReference type="CTD" id="83872"/>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
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                <person name="Zhang Y."/>
                <person name="Zhou T."/>
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                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
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                <person name="Yuan Z."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
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            <authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Insulin-like_neuro_ligands"/>
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        <property type="entry name" value="Insulin_CS"/>
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    <dbReference type="PANTHER" id="PTHR20968">
        <property type="entry name" value="ILGF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RELAXIN-3"/>
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    <dbReference type="Pfam" id="PF00049">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="IlGF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Insulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="INSULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0372">Hormone</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039886028" description="Relaxin-3" evidence="11">
        <location>
            <begin position="20"/>
            <end position="152"/>
        </location>
    </feature>
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        <location>
            <begin position="27"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00040355"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000406"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00078"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_053530612.1"/>
        </source>
    </evidence>
    <sequence length="152" mass="16540" checksum="F4AD4664508780D6" modified="2023-06-28" version="1">MMWKAMVLAVCLLMAGVRAYDGPSYGVKLCGREFIRAVIFTCGGSRWRRSITSTDDLSVDPFGSGIRDAAEGLVLPSHPKFSLQHDTEPISFDMVRGGQEASEFSRPARSIISDEVLEALRTSSRKGRDVVVGLSNACCKWGCSKSEISSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TFI4</accession>
    <name>A0A9F7TFI4_ICTPU</name>
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        <submittedName>
            <fullName evidence="10 11 12">Transcriptional repressor p66-beta isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 11 12" type="primary">gatad2b</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11 12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11 12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_053675326.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_053675327.1"/>
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        <property type="nucleotide sequence ID" value="XM_053675328.1"/>
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    <dbReference type="GeneTree" id="ENSGT00390000004097"/>
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        <property type="component" value="Chromosome 24"/>
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        <property type="term" value="C:NuRD complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="entry name" value="P66"/>
    </dbReference>
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        <property type="entry name" value="P66_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13455:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR P66-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13455">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR P66-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16563">
        <property type="entry name" value="P66_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="8">
        <location>
            <begin position="491"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="15"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="140"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="325"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="165"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="197"/>
            <end position="209"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053531301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053531302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053531303.1"/>
        </source>
    </evidence>
    <sequence length="693" mass="75417" checksum="189F1D521267FDE6" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
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    <name>A0A9F7TGN8_ICTPU</name>
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        <submittedName>
            <fullName evidence="7">Zinc finger protein 804A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">znf804a</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053536594.1">
        <property type="nucleotide sequence ID" value="XM_053680619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TGN8"/>
    <dbReference type="GeneID" id="108266150"/>
    <dbReference type="KEGG" id="ipu:108266150"/>
    <dbReference type="CTD" id="91752"/>
    <dbReference type="OrthoDB" id="4822at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052445">
        <property type="entry name" value="ZnF-G_patch_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17614:SF13">
        <property type="entry name" value="ZINC FINGER PROTEIN 804A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17614">
        <property type="entry name" value="ZINC FINGER-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="5">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="258"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="365"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="418"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="564"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="618"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="665"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="368"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="444"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="517"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="567"/>
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                <person name="Liu S."/>
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                <person name="Bao L."/>
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                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
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                <person name="Zhang Y."/>
                <person name="Zhou T."/>
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                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
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                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
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                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
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                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
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                <person name="Kucuktas H."/>
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                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
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                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
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        <property type="term" value="P:intracellular signal transduction"/>
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        <property type="term" value="P:negative regulation of signal transduction"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0008277">
        <property type="term" value="P:regulation of G protein-coupled receptor signaling pathway"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="DEP_RGS7-like"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Regulator of G-protein signaling 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1240.60:FF:000001">
        <property type="entry name" value="Regulator of G-protein signaling 6"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Regulator of G-protein signaling 6"/>
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        <property type="entry name" value="Regulator of G-protein signaling 6 isoform 9"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
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        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
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        <property type="entry name" value="GGL_sf"/>
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    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047016">
        <property type="entry name" value="RGS6/7/9/11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047017">
        <property type="entry name" value="RGS6/7/9/11_DHEX_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040759">
        <property type="entry name" value="RGS_DHEX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45746">
        <property type="entry name" value="LP21163P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45746:SF7">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 7"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
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    <dbReference type="Pfam" id="PF18148">
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    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
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        <property type="entry name" value="GGL"/>
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    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF48670">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0734">Signal transduction inhibitor</keyword>
    <feature type="domain" description="DEP" evidence="9">
        <location>
            <begin position="37"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="8">
        <location>
            <begin position="333"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
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    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053541259.1"/>
        </source>
    </evidence>
    <sequence length="459" mass="53715" checksum="B61E9562722A16B6" modified="2023-06-28" version="1">MAQTNSYGQSSNGVADESPNMLVYRKMEDIIARMQEEKNGIPIRTVKSFLSKIPSVFSGSDIVQWMIKNLAIEDQVEALHLGTLMSAHGYFFPISDHVLTLKDDGTFYRFQTPYFWPSNCWEPENTDYAVYLCKRTMQNKARLELADYEAESLARLQRAFARKWEFIFMQAEAQAKVDKKRDKIERKILDSQERAFWDVHRPVPGCVNTTEADIKKSSRMKQPHKTRKSVYGLQNDVRSHSPTHTSTPEVKEPTEEELQELIQYWQAQLDRHRLKMSKVAESLLSYTEQYMEYDPFITSPELSNPWISDDTALWELEASKEPGQQRVRRWGFNFDEVLKDPVGREQFLKFLESEFSSENLCFWLAVQELKKRPIREVPSRVQEIWDEFLASGAPSAINLDSKSYDKTMQNVKDPGRYTFEDAQEHIYKLMKSDSYSRFIRSSAYQELLQAKKKKSKNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TMF7</accession>
    <name>A0A9F7TMF7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein LOC128628728</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC128628728</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053538156.1">
        <property type="nucleotide sequence ID" value="XM_053682181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TMF7"/>
    <dbReference type="GeneID" id="128628728"/>
    <dbReference type="KEGG" id="ipu:128628728"/>
    <dbReference type="OrthoDB" id="8924181at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF111">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039905360" evidence="9">
        <location>
            <begin position="20"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="32"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053538156.1"/>
        </source>
    </evidence>
    <sequence length="271" mass="30541" checksum="FBFE2AA81C1A52F5" modified="2023-06-28" version="1">MTSTWILILIFSTMYTVQPGRRWVTAQFLTKPPVYQPDKELSVNSGDSATLQCCVSVNEVVIIVWFKQPKRKKPDPIVRVYKSGLDAFYNESQKSHFQIERSSNCINLIILNTAQSDEAMYYCALTFPNTVFADGTYLKIKGDHVTTASETSKPALCDNSVVCEPTLHGNNTNVNTHEKTVLGLGTALGLCTLLIFCLIYFTLRRRKCDKTCIEDSPGMKQVRESEAETLNYAAVQFTKRKAKAEKKKNSSADECVYADVKKTVRYNRNNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TMI8</accession>
    <name>A0A9F7TMI8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">MAGUK p55 subfamily member 7 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">mpp7a</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053540813.1">
        <property type="nucleotide sequence ID" value="XM_053684838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TMI8"/>
    <dbReference type="GeneID" id="108273933"/>
    <dbReference type="KEGG" id="ipu:108273933"/>
    <dbReference type="CTD" id="30166"/>
    <dbReference type="OrthoDB" id="439127at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06799">
        <property type="entry name" value="PDZ_MPP3-MPP4-MPP7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000002">
        <property type="entry name" value="Guanylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.650">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014775">
        <property type="entry name" value="L27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036892">
        <property type="entry name" value="L27_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050716">
        <property type="entry name" value="MAGUK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23122">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE MAGUK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02828">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00569">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101288">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51022">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="L27" evidence="8">
        <location>
            <begin position="10"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="L27" evidence="8">
        <location>
            <begin position="65"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="139"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="228"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="6">
        <location>
            <begin position="367"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="301"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="301"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053540813.1"/>
        </source>
    </evidence>
    <sequence length="575" mass="64254" checksum="957E7739D03CB764" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3TEC8</accession>
    <name>E3TEC8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein Churchill</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CHUR</name>
        <name evidence="11" type="synonym">chur</name>
        <name evidence="11" type="synonym">churc1</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Churchill family.</text>
    </comment>
    <dbReference type="EMBL" id="GU588708">
        <property type="protein sequence ID" value="ADO28664.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001188076.1">
        <property type="nucleotide sequence ID" value="NM_001201147.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TEC8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029392"/>
    <dbReference type="GeneID" id="100528877"/>
    <dbReference type="KEGG" id="ipu:100528877"/>
    <dbReference type="CTD" id="91612"/>
    <dbReference type="GeneTree" id="ENSGT00390000011163"/>
    <dbReference type="OMA" id="ASHEYTF"/>
    <dbReference type="OrthoDB" id="5954706at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008543">
        <property type="term" value="P:fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.4240:FF:000001">
        <property type="entry name" value="Churchill domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4240">
        <property type="entry name" value="Transcription activator, Churchill"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038543">
        <property type="entry name" value="Churchill_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009508">
        <property type="entry name" value="Transcrpt_activator_Churchill"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31931">
        <property type="entry name" value="PROTEIN CHURCHILL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31931:SF2">
        <property type="entry name" value="PROTEIN CHURCHILL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06573">
        <property type="entry name" value="Churchill"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADO28664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="NP_001188076.1"/>
        </source>
    </evidence>
    <sequence length="112" mass="12823" checksum="A70DA36EE895E964" modified="2011-01-11" version="1">MCTGCVEKEYPDRGNTCLDNGSYLMNFLGCANCQQRDFVLISNKTLVDEDEEEIVTYLHKCKNCDHVIARHEYTFSVVDDYQEYTMLCMLCGKAEDSISVMPDDPRQSAPLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3TEW7</accession>
    <name>E3TEW7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Complex I-SGDH</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">NADH-ubiquinone oxidoreductase SGDH subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">NDUB5</name>
        <name evidence="21" type="synonym">ndub5</name>
        <name evidence="21" type="synonym">ndufb5</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 21" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2016" name="Biol. Reprod." volume="95" first="19" last="19">
            <title>Transcriptome Display During Testicular Differentiation of Channel Catfish (Ictalurus punctatus) as Revealed by RNA-Seq Analysis.</title>
            <authorList>
                <person name="Zeng Q."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Zhang Y."/>
                <person name="Yuan Z."/>
                <person name="Jiang C."/>
                <person name="Chen A."/>
                <person name="Fu Q."/>
                <person name="Su B."/>
                <person name="Dunham R."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="27307075"/>
            <dbReference type="DOI" id="10.1095/biolreprod.116.138818"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="4">
        <citation type="submission" date="2022-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Complex I is composed of 45 different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFB5 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GU588900">
        <property type="protein sequence ID" value="ADO28853.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187586.1">
        <property type="nucleotide sequence ID" value="NM_001200657.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187586.1">
        <property type="nucleotide sequence ID" value="NM_001200657.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TEW7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013284"/>
    <dbReference type="GeneID" id="100528387"/>
    <dbReference type="KEGG" id="ipu:100528387"/>
    <dbReference type="CTD" id="4711"/>
    <dbReference type="OrthoDB" id="9995605at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019173">
        <property type="entry name" value="NADH_UbQ_OxRdtase_B5_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13178:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13178">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE SGDH SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09781">
        <property type="entry name" value="NDUF_B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="19" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="127"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00032550"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ADO28853.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="NP_001187586.1"/>
        </source>
    </evidence>
    <sequence length="186" mass="21576" checksum="880CB231D95C12B1" modified="2011-01-11" version="1">MAALSVLRAVAAFAARINPLKRVNQSSDLLSRAVVRSEKVAVRYGSHGKRMFVIKPTDFYDRRFLKLLQYYILLTGIPVAAFITFVNVFIGEAELVEVPEGYEPEHWEYYKHPITRWIARNIYDSPVKDYEKMMALIQIEAEKAELRQKQLEVRKHMRAKGDGPWFYIDTLDKNLIDQSPKATPDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3TEZ1</accession>
    <name>E3TEZ1_ICTPU</name>
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        <recommendedName>
            <fullName evidence="2">STING ER exit protein</fullName>
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    <gene>
        <name evidence="5" type="primary">CX056</name>
        <name evidence="7" type="synonym">c28hxorf56</name>
        <name evidence="7" type="synonym">cx056</name>
        <name evidence="7" type="synonym">steep1</name>
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    <organism evidence="5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="5 7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="journal article" date="2016" name="Biol. Reprod." volume="95" first="19" last="19">
            <title>Transcriptome Display During Testicular Differentiation of Channel Catfish (Ictalurus punctatus) as Revealed by RNA-Seq Analysis.</title>
            <authorList>
                <person name="Zeng Q."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Zhang Y."/>
                <person name="Yuan Z."/>
                <person name="Jiang C."/>
                <person name="Chen A."/>
                <person name="Fu Q."/>
                <person name="Su B."/>
                <person name="Dunham R."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="27307075"/>
            <dbReference type="DOI" id="10.1095/biolreprod.116.138818"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="5">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Molecular adapter that stimulates membrane curvature formation and subsequent endoplasmic reticulum exit site (ERES) establishment by recruiting PI3K complex I, leading to COPII vesicle-mediated transport. Promotes endoplasmic reticulum (ER) exit of cGAMP-activated STING1 oligomers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the STEEP1 family.</text>
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        <property type="protein sequence ID" value="ADO28877.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NM_001200677.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187606.1">
        <property type="nucleotide sequence ID" value="NM_001200677.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TEZ1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000024279"/>
    <dbReference type="GeneID" id="100528407"/>
    <dbReference type="KEGG" id="ipu:100528407"/>
    <dbReference type="CTD" id="63932"/>
    <dbReference type="OrthoDB" id="418131at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
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        <property type="term" value="P:endoplasmic reticulum membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029704">
        <property type="entry name" value="STEEP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057965">
        <property type="entry name" value="STEEP1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46355:SF1">
        <property type="entry name" value="STING ER EXIT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46355">
        <property type="entry name" value="UPF0428 PROTEIN CXORF56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25809">
        <property type="entry name" value="STEEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STEEP1" evidence="4">
        <location>
            <begin position="22"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00046019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADO28877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="NP_001187606.1"/>
        </source>
    </evidence>
    <sequence length="224" mass="25753" checksum="6FC4CB969827AC24" modified="2011-01-11" version="1">MPKVVSRSVVCSDTRDREEYDDGEKPLHVYYCLCGQMVLVLDCQLEKLPMWPRDKARVIDAAKHAHKFCNVEEDESVYLKRPEGIERQYRKKCGKCGLLLFYQHQLKNTPVTLIVFDAVVKFGEGFGNTSVYSQKQETPKKVRVMMTKRTKDMGKFSSVTVSTIDEEEEEIEAREVADSYAQNAKVIEKQLEGKGMSKKRLQELAELEAKKAKMKGTLIDNQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3TFI5</accession>
    <name>E3TFI5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Ribonuclease P protein subunit p20</fullName>
            <shortName evidence="11">RNaseP protein p20</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">POP7</name>
        <name evidence="15" type="synonym">pop7</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="journal article" date="2016" name="Biol. Reprod." volume="95" first="19" last="19">
            <title>Transcriptome Display During Testicular Differentiation of Channel Catfish (Ictalurus punctatus) as Revealed by RNA-Seq Analysis.</title>
            <authorList>
                <person name="Zeng Q."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Zhang Y."/>
                <person name="Yuan Z."/>
                <person name="Jiang C."/>
                <person name="Chen A."/>
                <person name="Fu Q."/>
                <person name="Su B."/>
                <person name="Dunham R."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="27307075"/>
            <dbReference type="DOI" id="10.1095/biolreprod.116.138818"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="14" key="4">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="5">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8 11">Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of nuclear RNase P and RNase MRP complexes. RNase P consists of a catalytic RNA moiety and 10 different protein chains; POP1, POP4, POP5, POP7, RPP14, RPP21, RPP25, RPP30, RPP38 and RPP40. Within the RNase P complex, POP1, POP7 and RPP25 form the 'finger' subcomplex, POP5, RPP14, RPP40 and homodimeric RPP30 form the 'palm' subcomplex, and RPP21, POP4 and RPP38 form the 'wrist' subcomplex. All subunits of the RNase P complex interact with the catalytic RNA. Several subunits of RNase P are also part of the RNase MRP complex. RNase MRP consists of a catalytic RNA moiety and about 8 protein subunits; POP1, POP7, RPP25, RPP30, RPP38, RPP40 and possibly also POP4 and POP5. Interacts with SMN1. POP7 forms a heterodimer with RPP25 that binds to the P3 stem loop of the catalytic RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 11">Nucleus</location>
            <location evidence="2 11">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the histone-like Alba family.</text>
    </comment>
    <dbReference type="EMBL" id="GU589119">
        <property type="protein sequence ID" value="ADO29071.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187749.1">
        <property type="nucleotide sequence ID" value="NM_001200820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TFI5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000543"/>
    <dbReference type="GeneID" id="100528550"/>
    <dbReference type="KEGG" id="ipu:100528550"/>
    <dbReference type="CTD" id="10248"/>
    <dbReference type="GeneTree" id="ENSGT00510000048483"/>
    <dbReference type="OMA" id="EVFLHCT"/>
    <dbReference type="OrthoDB" id="416729at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.20:FF:000002">
        <property type="entry name" value="Ribonuclease P protein subunit p20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014612">
        <property type="entry name" value="Pop7/Rpp20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15314">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15314:SF1">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12328">
        <property type="entry name" value="Rpp20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036572">
        <property type="entry name" value="RPP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6 11" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR036572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADO29071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="NP_001187749.1"/>
        </source>
    </evidence>
    <sequence length="151" mass="16667" checksum="B4C0EF2D68ABA048" modified="2011-01-11" version="1">MAEPRSQVSTSMSQKSMGFSASDGPATEMDPVEYTLRKRLPRKLPKRRNDVYINMKTDFKAQLARCQKLLDGSAGHKEICIHGLGLAINRAINIALQLQAFSQGAIQLAANTSTVELIDDLEPEDPDEAGEPLTRTRNNSAIHIKVFYPNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3TGN2</accession>
    <name>E3TGN2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Elongation of very long chain fatty acids protein 5</fullName>
            <ecNumber evidence="21">2.3.1.199</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">3-keto acyl-CoA synthase ELOVL5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">ELOVL fatty acid elongase 5</fullName>
            <shortName evidence="21">ELOVL FA elongase 5</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Very long chain 3-ketoacyl-CoA synthase 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Very long chain 3-oxoacyl-CoA synthase 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">ELOV5</name>
        <name evidence="26" type="synonym">elov5</name>
        <name evidence="21" type="synonym">ELOVL5</name>
        <name evidence="26" type="synonym">elovl5</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 26" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">SDA103</strain>
        </source>
    </reference>
    <reference evidence="26" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that acts specifically toward polyunsaturated acyl-CoA with the higher activity toward C18:3(n-6) acyl-CoA. May participate in the production of monounsaturated and of polyunsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. In conditions where the essential linoleic and alpha linoleic fatty acids are lacking it is also involved in the synthesis of Mead acid from oleic acid.</text>
    </comment>
    <comment type="function">
        <text evidence="21">Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that acts specifically toward polyunsaturated acyl-CoA with the higher activity toward C18:3(n-6) acyl-CoA. May participate to the production of monounsaturated and of polyunsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(11Z)-octadecenoyl-CoA + malonyl-CoA + H(+) = 3-oxo-(13Z)-eicosenoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:39679"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:75121"/>
            <dbReference type="ChEBI" id="CHEBI:76559"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:39680"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + malonyl-CoA + H(+) = (7Z,10Z,13Z,16Z)-3-oxodocosatetraenoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:36475"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:73852"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:36476"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(6Z,9Z,12Z)-octadecatrienoyl-CoA + malonyl-CoA + H(+) = 3-oxo-(8Z,11Z,14Z)-eicosatrienoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:35379"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57363"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:71481"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:35380"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(6Z,9Z,12Z,15Z)-octadecatetraenoyl-CoA + malonyl-CoA + H(+) = 3-oxo-(8Z,11Z,14Z,17Z)-eicosatetraenoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:35391"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:71489"/>
            <dbReference type="ChEBI" id="CHEBI:71491"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:35392"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(9Z)-hexadecenoyl-CoA + malonyl-CoA + H(+) = 3-oxo-(11Z)-octadecenoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:39675"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:61540"/>
            <dbReference type="ChEBI" id="CHEBI:76555"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:39676"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(9Z)-octadecenoyl-CoA + malonyl-CoA + H(+) = 3-oxo-(11Z)-eicosenoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:36511"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:74011"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:36512"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(9Z,12Z)-octadecadienoyl-CoA + malonyl-CoA + H(+) = (11Z,14Z)-3-oxoicosa-11,14-dienoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:36503"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57383"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:74012"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:36504"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(9Z,12Z,15Z)-octadecatrienoyl-CoA + malonyl-CoA + H(+) = (11Z,14Z,17Z)-3-oxoeicosatrienoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:36523"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:74034"/>
            <dbReference type="ChEBI" id="CHEBI:74054"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:36524"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21 22">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="21">Lipid metabolism; polyunsaturated fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="21">Endoplasmic reticulum membrane</location>
            <topology evidence="21">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Cell projection</location>
            <location evidence="21">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="21">In Purkinje cells, the protein localizes to the soma and proximal portion of the dendritic tree.</text>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the ELO family. ELOVL5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.199" evidence="21"/>
    <dbReference type="EMBL" id="GU589518">
        <property type="protein sequence ID" value="ADO29468.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001188041.1">
        <property type="nucleotide sequence ID" value="NM_001201112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TGN2"/>
    <dbReference type="GeneID" id="100528842"/>
    <dbReference type="KEGG" id="ipu:100528842"/>
    <dbReference type="CTD" id="60481"/>
    <dbReference type="OrthoDB" id="434092at2759"/>
    <dbReference type="UniPathway" id="UPA00658"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035338">
        <property type="term" value="P:long-chain fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03205">
        <property type="entry name" value="VLCF_elongase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033677">
        <property type="entry name" value="ELOVL5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF18">
        <property type="entry name" value="ELONGATION OF VERY LONG CHAIN FATTY ACIDS PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="11 21" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5 21" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 21 22" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6 21 22" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 21 22" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 21 22" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 21 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 21 22" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 21 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 21 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21 22">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21 22">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21 22">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21 22">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21 22">
        <location>
            <begin position="201"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21 22">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="259"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00052042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="ADO29468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="NP_001188041.1"/>
        </source>
    </evidence>
    <sequence length="288" mass="34270" checksum="02A58C52E5940363" modified="2011-01-11" version="1">MLNKHIDSWMGPRDPRVRGWLLLDNYPPTLAFTIMYLLIVWMGPKYMRNRQPFSCRGILVLYNLVLTFLSFCMFYELVTAVWQGGYNFFCQDIHSGGEADDRIIHVLWWYYFSKLIEFMDTFFFILRKNNHQITFLHIYHHATMPNIWWFVMNWVPCGHSYFGASFNSFIHVLMYSYYGLSAVPAMRPYLWWKKYITQGQLIQFVLTMIQTSCAVVWPCGFPMGWLYFQISYMITLIMFFLNFYIQTYKRPGAGLKKDHRHNGVKSANGHSNGATHTDMVKHRKPRAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5U6F6</accession>
    <name>W5U6F6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endoplasmic reticulum membrane sensor NFE2L1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Nuclear factor erythroid 2-related factor 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Nuclear factor, erythroid derived 2, like 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">NFE2L1</name>
        <name evidence="29 30 31 32" type="synonym">nfe2l1b</name>
    </gene>
    <organism evidence="27">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="27">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="28" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">SDA103</strain>
        </source>
    </reference>
    <reference evidence="29 30 31" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="29 30 31">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type III membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the bZIP family. CNC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="JT406206">
        <property type="protein sequence ID" value="AHH37498.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017338557.1">
        <property type="nucleotide sequence ID" value="XM_017483068.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017338558.1">
        <property type="nucleotide sequence ID" value="XM_017483069.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053541370.1">
        <property type="nucleotide sequence ID" value="XM_053685395.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053541371.1">
        <property type="nucleotide sequence ID" value="XM_053685396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U6F6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026889"/>
    <dbReference type="GeneID" id="108273673"/>
    <dbReference type="KEGG" id="ipu:108273673"/>
    <dbReference type="CTD" id="558772"/>
    <dbReference type="GeneTree" id="ENSGT00950000182892"/>
    <dbReference type="OrthoDB" id="7458135at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14720">
        <property type="entry name" value="bZIP_NFE2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.880.10:FF:000001">
        <property type="entry name" value="Nuclear factor erythroid 2-related factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.880.10">
        <property type="entry name" value="Transcription factor, Skn-1-like, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004826">
        <property type="entry name" value="bZIP_Maf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047167">
        <property type="entry name" value="NFE2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008917">
        <property type="entry name" value="TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24411:SF31">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MEMBRANE SENSOR NFE2L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24411">
        <property type="entry name" value="NUCLEAR FACTOR ERYTHROID 2-RELATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03131">
        <property type="entry name" value="bZIP_Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47454">
        <property type="entry name" value="A DNA-binding domain in eukaryotic transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="17" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="20" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="21" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="19" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="18" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="BZIP" evidence="26">
        <location>
            <begin position="683"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="115"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="292"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="350"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="490"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="781"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="146"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="211"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="350"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="503"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="25">
        <location>
            <begin position="792"/>
            <end position="801"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00030985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00031659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="AHH37498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_017338557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="RefSeq" id="XP_017338558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="RefSeq" id="XP_053541370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="RefSeq" id="XP_053541371.1"/>
        </source>
    </evidence>
    <sequence length="801" mass="88711" checksum="192D20BEB3FCFBE9" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5U8C5</accession>
    <name>W5U8C5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like modifier-activating enzyme 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">UFM1-activating enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">UBA5</name>
        <name evidence="12 13" type="synonym">uba5</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12 13">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ubiquitin-activating E1 family. UBA5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="JT406940">
        <property type="protein sequence ID" value="AHH37789.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JT414063">
        <property type="protein sequence ID" value="AHH40483.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017322723.1">
        <property type="nucleotide sequence ID" value="XM_017467234.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053535738.1">
        <property type="nucleotide sequence ID" value="XM_053679763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U8C5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000002568"/>
    <dbReference type="GeneID" id="108265114"/>
    <dbReference type="KEGG" id="ipu:108265114"/>
    <dbReference type="CTD" id="79876"/>
    <dbReference type="GeneTree" id="ENSGT00940000156177"/>
    <dbReference type="OMA" id="MNIVKDY"/>
    <dbReference type="OrthoDB" id="206053at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071566">
        <property type="term" value="F:UFM1 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071569">
        <property type="term" value="P:protein ufmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00757">
        <property type="entry name" value="ThiF_MoeB_HesA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000066">
        <property type="entry name" value="Putative ubiquitin-like modifier-activating enzyme 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF9">
        <property type="entry name" value="UBIQUITIN-LIKE MODIFIER-ACTIVATING ENZYME 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="9">
        <location>
            <begin position="52"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AHH37789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017322723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_053535738.1"/>
        </source>
    </evidence>
    <sequence length="401" mass="44808" checksum="D7F1781E08242B20" modified="2014-04-16" version="1">MATVEELKLRIRELENELIKCKQQRSQENLPNPVLRAKIEKMSAEVVDSNPYSRLMALKRMGIVEDYEKIRSFAVAVVGVGGVGSVTAEMLTRCGIGKLLLFDYDKVELANMNRLFFQPHQAGLSKVEAAEHTLRNINPDVIFETHNYNITTMDNFTHFMDRISNGGLQEGKRVDLVLSCVDNFEARMAINTACNELGQTWMESGVSENAVSGHIQLIIPGETACFACAPPLVVAANIDEKTLKKDGVCAASLPTTMGVVAGILVQNVLKYLLRFGTVSFYLGYNAMQDFFPTMSMKANPQCDDRHCRRQQEEYIKKEAEHPKQEVVEEEEEVVHEENEWGIELVSEITVAELQDASGPKPDLPEGITVAYTIPNKDVKSGETVEETDQSLEELMAQMKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5U8N6</accession>
    <name>W5U8N6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">H-RAS</name>
        <name evidence="14" type="synonym">zgc:55558</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="EMBL" id="JT408294">
        <property type="protein sequence ID" value="AHH38293.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017313508.1">
        <property type="nucleotide sequence ID" value="XM_017458019.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U8N6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034432"/>
    <dbReference type="GeneID" id="108258948"/>
    <dbReference type="GeneTree" id="ENSGT00940000155653"/>
    <dbReference type="OMA" id="NQRRCII"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04138">
        <property type="entry name" value="H_N_K_Ras_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000096">
        <property type="entry name" value="KRAS proto-oncogene, GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AHH38293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017313508.1"/>
        </source>
    </evidence>
    <sequence length="187" mass="21406" checksum="415DB63A20B06BA0" modified="2014-04-16" version="1">MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNIKSFEDVHLYREQINRVKDSDNVPMVLVGNKSDLSSRTVETRQAQELARGYGIPFVETSAKTRQGVEEAFYSLVREIRRYKETNRSNKKSKKHPQRRCILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5UC28</accession>
    <name>W5UC28_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Divergent protein kinase domain 1A</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="11">Protein FAM69A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">fam69a</name>
        <name evidence="13" type="synonym">dipk1aa</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DIPK family.</text>
    </comment>
    <dbReference type="EMBL" id="JT411725">
        <property type="protein sequence ID" value="AHH39576.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017351620.1">
        <property type="nucleotide sequence ID" value="XM_017496131.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UC28"/>
    <dbReference type="GeneID" id="108280758"/>
    <dbReference type="KEGG" id="ipu:108280758"/>
    <dbReference type="CTD" id="678606"/>
    <dbReference type="OMA" id="TMRVLKC"/>
    <dbReference type="OrthoDB" id="8860232at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022049">
        <property type="entry name" value="FAM69_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029244">
        <property type="entry name" value="FAM69_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21093:SF8">
        <property type="entry name" value="DIVERGENT PROTEIN KINASE DOMAIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21093">
        <property type="entry name" value="DIVERGENT PROTEIN KINASE DOMAIN 1C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12260">
        <property type="entry name" value="PIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14875">
        <property type="entry name" value="PIP49_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01299">
        <property type="entry name" value="PIP49_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="FAM69 N-terminal" evidence="10">
        <location>
            <begin position="19"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AHH39576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017351620.1"/>
        </source>
    </evidence>
    <sequence length="428" mass="48412" checksum="887C2794DE3FC9A1" modified="2014-04-16" version="1">MARGLFARAWITKSYLFHVRLSYVRVKYLFLTWLAVFVGSWVVYVQYSSYTELCRAHECYSTICDKYRAGVIDGAACSSLCEKASLYFRRCLSSKPNNQVYTASWGDVEAVIRCWPGDVLHYELGEELEPRKEPTLFDKPTRGTSVEKFKEMVHGHVKAKVGEQADLTTLVSLVLSFADADNDGRVSLPEARSAWALLQLDEVLLSVVLQGRSHTPKLLGFCGDLYVVERVAYTPLYGLDLSPALKPWITAVVGRGLDHFFAPSWPRKVKISIGLLELVEDVFHGTFGSFLLCDMKPGSFGYTERYELRLLDGRHVVPEEAFRRVMRVRPCQEDSDCVYSGECRASCRRTERRCGAEPSRTNLARACSALEDFLLQGAPASLRDELERQLNACMELTGSGEQMQMEHSLILNNLKMLLWKQISHTIDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5UCK6</accession>
    <name>W5UCK6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cytochrome P450 1A</fullName>
            <ecNumber evidence="14">1.14.14.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">cyp1a1</name>
        <name evidence="17" type="synonym">LOC124627053</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an organic molecule + reduced [NADPH--hemoprotein reductase] + O2 = an alcohol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17149"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:142491"/>
            <dbReference type="EC" id="1.14.14.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12 14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endoplasmic reticulum membrane</location>
            <topology evidence="14">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Microsome membrane</location>
            <topology evidence="14">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.1" evidence="14"/>
    <dbReference type="EMBL" id="JT412024">
        <property type="protein sequence ID" value="AHH39698.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047009349.1">
        <property type="nucleotide sequence ID" value="XM_047153393.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UCK6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000015458"/>
    <dbReference type="KEGG" id="ipu:124627053"/>
    <dbReference type="GeneTree" id="ENSGT00950000183037"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004508">
        <property type="term" value="F:steroid 17-alpha-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042446">
        <property type="term" value="P:hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042448">
        <property type="term" value="P:progesterone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20676">
        <property type="entry name" value="CYP1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000002">
        <property type="entry name" value="Cytochrome P450 1A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008066">
        <property type="entry name" value="Cyt_P450_E_grp-I_CYP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24289:SF21">
        <property type="entry name" value="CYTOCHROME P450 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24289">
        <property type="entry name" value="STEROID 17-ALPHA-HYDROXYLASE/17,20 LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01683">
        <property type="entry name" value="EP450ICYP1A"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 12 13" id="KW-0349">Heme</keyword>
    <keyword evidence="8 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14" id="KW-0492">Microsome</keyword>
    <keyword evidence="9 13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="463"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU368045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AHH39698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_047009349.1"/>
        </source>
    </evidence>
    <sequence length="525" mass="59339" checksum="F60B0A0990053E32" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5UFD1</accession>
    <name>W5UFD1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7 9">C-myc promoter-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">DENND4A</name>
        <name evidence="9" type="synonym">dennd4a</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
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        <property type="protein sequence ID" value="AHH40688.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_017466610.3"/>
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    <dbReference type="GeneTree" id="ENSGT00940000155836"/>
    <dbReference type="OrthoDB" id="75250at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:regulation of Rab protein signal transduction"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="C-myc promoter-binding protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.100.10.50:FF:000001">
        <property type="entry name" value="DENN domain containing 4C"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="cDENN_dom"/>
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        <property type="entry name" value="dDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
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        <property type="entry name" value="DENN_Domain_GEFs"/>
    </dbReference>
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        <property type="entry name" value="MABP"/>
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    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
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    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296:SF16">
        <property type="entry name" value="C-MYC PROMOTER-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03455">
        <property type="entry name" value="dDENN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03456">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="43"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="UDENN" evidence="5">
        <location>
            <begin position="192"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="808"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="711"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="892"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1151"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1444"/>
            <end position="1479"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="718"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="902"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="937"/>
            <end position="951"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="952"/>
            <end position="979"/>
        </location>
    </feature>
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        <location>
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    </feature>
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            <begin position="1193"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1221"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1458"/>
            <end position="1467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50211"/>
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        <source>
            <dbReference type="PROSITE" id="PS51498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AHH40688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017322099.1"/>
        </source>
    </evidence>
    <sequence length="1720" mass="191042" checksum="EC416B6447EFBF07" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5UJ81</accession>
    <name>W5UJ81_ICTPU</name>
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        <recommendedName>
            <fullName evidence="3">Tektin</fullName>
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    <gene>
        <name evidence="6" type="primary">Tekt2</name>
        <name evidence="8" type="synonym">tekt2</name>
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    <organism evidence="6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the tektin family.</text>
    </comment>
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        <property type="protein sequence ID" value="AHH41745.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_017480823.3"/>
    </dbReference>
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    <dbReference type="OMA" id="FDHRGKM"/>
    <dbReference type="OrthoDB" id="440745at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048256">
        <property type="entry name" value="Tektin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000435">
        <property type="entry name" value="Tektins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19960">
        <property type="entry name" value="TEKTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19960:SF7">
        <property type="entry name" value="TEKTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03148">
        <property type="entry name" value="Tektin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00511">
        <property type="entry name" value="TEKTIN"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="392"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="240"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="392"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AHH41745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017336312.1"/>
        </source>
    </evidence>
    <sequence length="429" mass="49218" checksum="105B3736667CA710" modified="2014-04-16" version="1">MATLSIKPGLRCTVSDWMNTNNEISVTAEQRRHLSHEIRQEGRALCNDTTNKTIWGEYDNNRRLSDRISEVKLWKKSLEACAEEVDTEMDALTLTKEAVERALAATVLPLEVTNECLTLREGRRGNELLSDPVEAELKKEVEVIDKAQEVLQQCIDQAFRHLCLLQEARHQLTLDLQHKMQALDVDISCLSLSVTSSEISLKPNPTRVPPGSTTPQQWVQFSQNNITQAKEEMQASLHLRENINITIAEVQNELESQRIATRFAFRKRTHQLEQAHQELQWQIKTTQDEINELKEDILRLERDMQSKMAPLKLVHTRLEYRTRRPGTDLCRDEVQHGLVEETRQLASSILVLKQKLAQAQDSLQALQLHEARMLEDLVRKQEALSLEQRSMQTRQRLNSAQKESEPSAVSPLTNSSGMHKLQLKYTDSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>W5UJE8</accession>
    <name>W5UJE8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sorting nexin-16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">SNX16</name>
        <name evidence="20" type="synonym">snx16</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="14">Homooligomer. Interacts with EGFR.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Late endosome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT415770">
        <property type="protein sequence ID" value="AHH41815.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053539881.1">
        <property type="nucleotide sequence ID" value="XM_053683906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UJE8"/>
    <dbReference type="GeneID" id="108272138"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045022">
        <property type="term" value="P:early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006622">
        <property type="term" value="P:protein targeting to lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07276">
        <property type="entry name" value="PX_SNX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000011">
        <property type="entry name" value="Putative sorting nexin-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037911">
        <property type="entry name" value="SNX16_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051837">
        <property type="entry name" value="SortingNexin/PXDomain-PKLike"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22999">
        <property type="entry name" value="PX SERINE/THREONINE KINASE PXK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22999:SF23">
        <property type="entry name" value="SORTING NEXIN-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="13" id="KW-0458">Lysosome</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="17">
        <location>
            <begin position="100"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="14"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="273"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="282"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="313"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00063452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00071931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AHH41815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_053539881.1"/>
        </source>
    </evidence>
    <sequence length="339" mass="37553" checksum="2115DE739EE67639" modified="2014-04-16" version="1">MATPFVPVPVPIDQVLSASSGARRPKRASSVGSASSSSESSSTKGAAEDSGLGRSGNVSCFIASCQSPVTKARVNGVESTVEYSSCVRPDTPTDALGWEERPITPTVLGYEVMEERAKFTVYKILVKRTQDESWVVFRRYTDFSRLNDKLKEMFPGFRLALPPKRWFKDNYDTDFLEDRQLGLQAFLQNLVAHKDIANCVPVREFLCLDEPPGPFDSLEESRAFCETLEEGNYRLQKELLEKQKEISSLKKILEEKELYIHLLEKRINGESLTPESPYGLSAQGSESSVDAESSTAEADQDMPEETGCEHQASRSSSACWCGPTISSSPPVIQVTQVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5UKF8</accession>
    <name>W5UKF8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Latent-transforming growth factor beta-binding protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Transforming growth factor beta-1-binding protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">Ltbp1</name>
        <name evidence="22" type="synonym">LOC108269599</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 22" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space. Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta. Outcompeted by LRRC32/GARP for binding to LAP regulatory chain of TGF-beta.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with TGFB1; associates via disulfide bonds with the Latency-associated peptide chain (LAP) regulatory chain of TGFB1, leading to regulate activation of TGF-beta-1. LTBP1 does not bind directly to TGF-beta-1, the active chain of TGFB1. Interacts (via C-terminal domain) with FBN1 (via N-terminal domain). Interacts with FBN2. Interacts with ADAMTSL2. Interacts with EFEMP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the LTBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="JT413204">
        <property type="protein sequence ID" value="AHH40152.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053535332.1">
        <property type="nucleotide sequence ID" value="XM_053679357.1"/>
    </dbReference>
    <dbReference type="KEGG" id="ipu:108269599"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019838">
        <property type="term" value="F:growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000005">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000205">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000019">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 1 isoform X2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000046">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 1 isoform x2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000002">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000014">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000001">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000002">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000056">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000115">
        <property type="entry name" value="latent-transforming growth factor beta-binding protein 4 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.290.10">
        <property type="entry name" value="TGF-beta binding (TB) domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017878">
        <property type="entry name" value="TB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036773">
        <property type="entry name" value="TB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052080">
        <property type="entry name" value="vWF_C/EGF_Fibrillin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47333:SF4">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47333">
        <property type="entry name" value="VON WILLEBRAND FACTOR C AND EGF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00683">
        <property type="entry name" value="TB"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57581">
        <property type="entry name" value="TB module/8-cys domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51364">
        <property type="entry name" value="TB"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 17" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0340">Growth factor binding</keyword>
    <keyword evidence="11" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="69"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="280"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="19">
        <location>
            <begin position="394"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="459"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="19">
        <location>
            <begin position="512"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="709"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="833"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="874"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="915"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="999"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="19">
        <location>
            <begin position="1094"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="domain" description="TB" evidence="19">
        <location>
            <begin position="1267"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="1422"/>
            <end position="1466"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="284"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00062844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00072992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="AHH40152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_053535332.1"/>
        </source>
    </evidence>
    <sequence length="1477" mass="160532" checksum="35FEF23DF43B6668" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5ULZ5</accession>
    <name>W5ULZ5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sidoreflexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SFXN1</name>
        <name evidence="14 15" type="synonym">sfxn3</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mitochondrial serine transporter that mediates transport of serine into mitochondria, an important step of the one-carbon metabolism pathway. Mitochondrial serine is converted to glycine and formate, which then exits to the cytosol where it is used to generate the charged folates that serve as one-carbon donors.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-serine(in) = L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:35031"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sideroflexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="JT418459">
        <property type="protein sequence ID" value="AHH42745.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017319752.1">
        <property type="nucleotide sequence ID" value="XM_017464263.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017319753.1">
        <property type="nucleotide sequence ID" value="XM_017464264.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ULZ5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008183"/>
    <dbReference type="GeneID" id="108263463"/>
    <dbReference type="KEGG" id="ipu:108263463"/>
    <dbReference type="CTD" id="81855"/>
    <dbReference type="OMA" id="TIDDVWQ"/>
    <dbReference type="OrthoDB" id="6608471at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015075">
        <property type="term" value="F:monoatomic ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140300">
        <property type="term" value="P:serine import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004686">
        <property type="entry name" value="Mtc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00798">
        <property type="entry name" value="mtc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153">
        <property type="entry name" value="SIDEROFLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11153:SF20">
        <property type="entry name" value="SIDEROFLEXIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03820">
        <property type="entry name" value="SFXNs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AHH42745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017319752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017319753.1"/>
        </source>
    </evidence>
    <sequence length="322" mass="35400" checksum="03D69A8678CDED57" modified="2014-04-16" version="1">MSGDLPLNINIKEPQWDQSTFMGRAQHFFTVTDPRNVLLSNEVLEGARVIVEDYRKGIVKPGLTEDQLWRAKYIYDSAFHPDTGEKMLLIGRMSAQVPMNMTITGCMLTFYRTTPAVVFWQWVNQSFNAIVNYTNRSGDAPISVNQLGAAYVSATTGAVITALGLKSLTKHLPAIIGRFVPFAAVAAANCINIPFMRQRELKYGIPVTDEEGNRLGESASAAQQAIIQVVVSRIGMAVPAMAIPPVIMNALEKKAFMKRFPVLNAPVQVGLVGLCLVFATPLCCALFPQKSSMKLSRLEPDLQESIRQSSPNTTVVYFNKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5UQW7</accession>
    <name>W5UQW7_ICTPU</name>
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        <name evidence="22" type="primary">Antxr1</name>
        <name evidence="24" type="synonym">antxr1a</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
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    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton and thereby plays an important role in normal extracellular matrix (ECM) homeostasis. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells. May also act as a receptor for PLAU. Upon ligand binding, stimulates the phosphorylation of EGFR and ERK1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with gelatin and type 1 collagen. Interacts with the actin cytoskeleton.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cell projection</location>
            <location evidence="14">Filopodium membrane</location>
            <topology evidence="14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Cell projection</location>
            <location evidence="13">Lamellipodium membrane</location>
            <topology evidence="13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATR family.</text>
    </comment>
    <dbReference type="EMBL" id="JT416176">
        <property type="protein sequence ID" value="AHH41963.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017323066.1">
        <property type="nucleotide sequence ID" value="XM_017467577.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UQW7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009618"/>
    <dbReference type="GeneID" id="108265351"/>
    <dbReference type="CTD" id="793153"/>
    <dbReference type="OMA" id="ANNMRRP"/>
    <dbReference type="OrthoDB" id="10035766at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031527">
        <property type="term" value="C:filopodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031258">
        <property type="term" value="C:lamellipodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01474">
        <property type="entry name" value="vWA_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000017">
        <property type="entry name" value="Anthrax toxin receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008399">
        <property type="entry name" value="Anthrax_toxin_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008400">
        <property type="entry name" value="Anthrax_toxin_rcpt_extracel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16059">
        <property type="entry name" value="ANTHRAX TOXIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16059:SF11">
        <property type="entry name" value="ANTHRAX TOXIN RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05586">
        <property type="entry name" value="Ant_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05587">
        <property type="entry name" value="Anth_Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10 22 24" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 20" id="KW-0732">Signal</keyword>
    <keyword evidence="4 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540396" description="Anthrax toxin receptor 1" evidence="20">
        <location>
            <begin position="26"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="21">
        <location>
            <begin position="45"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="356"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="403"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="472"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="514"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="529"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="540"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="552"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="568"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00061894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00068139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00093334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="AHH41963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_017323066.1"/>
        </source>
    </evidence>
    <sequence length="602" mass="66466" checksum="A72984D6F75D83A1" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5UTH7</accession>
    <name>W5UTH7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rho guanine nucleotide exchange factor 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="28">Guanine nucleotide exchange factor H1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="34" type="primary">ARHGEF2</name>
        <name evidence="36" type="synonym">arhgef1a</name>
    </gene>
    <organism evidence="34">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="34">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="35" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="35">SDA103</strain>
        </source>
    </reference>
    <reference evidence="36" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="36">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
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        <subcellularLocation>
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        <location>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00031760"/>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50003"/>
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        <source>
            <dbReference type="PROSITE" id="PS50010"/>
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        <source>
            <dbReference type="PROSITE" id="PS50081"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="AHH42943.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="RefSeq" id="XP_017336844.1"/>
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    </evidence>
    <sequence length="927" mass="104835" checksum="7C1B290348539054" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PI37</accession>
    <name>A0A2D0PI37_ICTPU</name>
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        <submittedName>
            <fullName evidence="3">Aerolysin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108254737</name>
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    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017305499.2">
        <property type="nucleotide sequence ID" value="XM_017450010.3"/>
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    <dbReference type="KEGG" id="ipu:108254737"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="CDD" id="cd09302">
        <property type="entry name" value="Jacalin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20221">
        <property type="entry name" value="PFM_Dln1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.100.10.30">
        <property type="entry name" value="Jacalin-like lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.15.10">
        <property type="entry name" value="Proaerolysin, chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053280">
        <property type="entry name" value="Aerolysin-like_pore-former"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001229">
        <property type="entry name" value="Jacalin-like_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036404">
        <property type="entry name" value="Jacalin-like_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34007">
        <property type="entry name" value="AEROLYSIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34007:SF1">
        <property type="entry name" value="AEROLYSIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01419">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56973">
        <property type="entry name" value="Aerolisin/ETX pore-forming domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51752">
        <property type="entry name" value="JACALIN_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Jacalin-type lectin" evidence="1">
        <location>
            <begin position="4"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017305499.2"/>
        </source>
    </evidence>
    <sequence length="312" mass="34133" checksum="5E6C5EACECE9499F" modified="2023-06-28" version="2">MSYLSPILVIGGGGGKSFNFNGVGNGATLKKIWVWAGGWQIKAIKVWLTDGQIGQFGEPVGRFSEFSFEDGEHFTSLSLWGNGAGTRLGAIKFRTNRSREFFAQMTDWGLKTEYPIDVGSGICVGITGHSGADMDSLGFEFINTVKSTKLTNVQYPTLHDVIPNVAVEEIKSTTYENKSTQTQEYKLETSKTIIRKSSWSVTNKLEFTFSMEVKAGIPEVVEVSAGFGFKVGTESSYGLENSEERTELFSFPVKVPPGKTVHVDITIGRATVDLPYTGTVQITCYNGSVLEFPTSGIYKGLNYTDAKVVVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PKQ7</accession>
    <name>A0A2D0PKQ7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Vesicle-associated membrane protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">vamp3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017305640.1">
        <property type="nucleotide sequence ID" value="XM_017450151.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PKQ7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005421"/>
    <dbReference type="GeneID" id="108254815"/>
    <dbReference type="KEGG" id="ipu:108254815"/>
    <dbReference type="CTD" id="9341"/>
    <dbReference type="OMA" id="MWVILIA"/>
    <dbReference type="OrthoDB" id="10042941at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15870">
        <property type="entry name" value="R-SNARE_VAMP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000013">
        <property type="entry name" value="Vesicle-associated membrane protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016444">
        <property type="entry name" value="Synaptobrevin/VAMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45701">
        <property type="entry name" value="SYNAPTOBREVIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005409">
        <property type="entry name" value="Synaptobrevin_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00417">
        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="20"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017305640.1"/>
        </source>
    </evidence>
    <sequence length="105" mass="11710" checksum="8785811D3152CBA6" modified="2017-12-20" version="1">MSAPVGEGPGTSGIAGSNRRLQQTQAQVDEVVDIMRVNVDKVLERDQKLSELDDRADALQAGASQFETNAAKLKRKYWWKNCKMWAILIAVLVIIIVIIIIWSNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PMA4</accession>
    <name>A0A2D0PMA4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Coilin isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">coil</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017306556.1">
        <property type="nucleotide sequence ID" value="XM_017451067.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PMA4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000001803"/>
    <dbReference type="GeneID" id="108255243"/>
    <dbReference type="KEGG" id="ipu:108255243"/>
    <dbReference type="CTD" id="8161"/>
    <dbReference type="OMA" id="CEYKKQT"/>
    <dbReference type="OrthoDB" id="74813at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030576">
        <property type="term" value="P:Cajal body organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024822">
        <property type="entry name" value="Coilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031722">
        <property type="entry name" value="Coilin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056398">
        <property type="entry name" value="Tudor_Coilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15197:SF0">
        <property type="entry name" value="COILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15197">
        <property type="entry name" value="COILIN P80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15862">
        <property type="entry name" value="Coilin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23086">
        <property type="entry name" value="Tudor_Coilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coilin N-terminal" evidence="2">
        <location>
            <begin position="20"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Coilin tudor" evidence="3">
        <location>
            <begin position="550"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="442"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="482"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="266"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="385"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="498"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017306556.1"/>
        </source>
    </evidence>
    <sequence length="659" mass="71909" checksum="119050C70A5C933E" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PN36</accession>
    <name>A0A2D0PN36_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fibroblast growth factor</fullName>
            <shortName evidence="4">FGF</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">fgf5</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017306831.1">
        <property type="nucleotide sequence ID" value="XM_017451342.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PN36"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013557"/>
    <dbReference type="GeneID" id="108255401"/>
    <dbReference type="KEGG" id="ipu:108255401"/>
    <dbReference type="CTD" id="2250"/>
    <dbReference type="OMA" id="TQVKYWP"/>
    <dbReference type="OrthoDB" id="9947297at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23317">
        <property type="entry name" value="beta-trefoil_FGF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00263">
        <property type="entry name" value="HBGFFGF"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00442">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00247">
        <property type="entry name" value="HBGF_FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5011827528" description="Fibroblast growth factor" evidence="4">
        <location>
            <begin position="19"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU049442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017306831.1"/>
        </source>
    </evidence>
    <sequence length="215" mass="24587" checksum="BB41E226BC43E6D8" modified="2017-12-20" version="1">MNVALLLVLVAQLIGTAAVWLEDHLVASGRRTGTLYCRVGIGFHLQIHPNGRVNGSHEPSNLSLLELFSISQGVIGIRGVFSNRFLAMNKRGWLHATEKFTDDCKFRERFQENSYNTYASVIHKNHRTGREWFVALNKRGKAKMGSSPRVKSQHVSTHFLPRMNLHMKLQQGFTITDKTQERTQALPKPRAPKVTMNMGQKQTRTVKYWPKFRFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PRH2</accession>
    <name>A0A2D0PRH2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">H(+)-transporting two-sector ATPase</fullName>
            <ecNumber evidence="2">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">atp6v1ab</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. May play a role in neurite development and synaptic connectivity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017309168.1">
        <property type="nucleotide sequence ID" value="XM_017453679.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PRH2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000014493"/>
    <dbReference type="GeneID" id="108256618"/>
    <dbReference type="KEGG" id="ipu:108256618"/>
    <dbReference type="CTD" id="337583"/>
    <dbReference type="OMA" id="RIVKTFW"/>
    <dbReference type="OrthoDB" id="1676488at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033180">
        <property type="term" value="C:proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042592">
        <property type="term" value="P:homeostatic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18111">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18119">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01134">
        <property type="entry name" value="V_A-ATPase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000008">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000052">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00309">
        <property type="entry name" value="ATP_synth_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031686">
        <property type="entry name" value="ATP-synth_a_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005725">
        <property type="entry name" value="ATPase_V1-cplx_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022878">
        <property type="entry name" value="V-ATPase_asu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003220">
        <property type="entry name" value="PRK04192.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01042">
        <property type="entry name" value="V-ATPase_V1_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607:SF1">
        <property type="entry name" value="H(+)-TRANSPORTING TWO-SECTOR ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607">
        <property type="entry name" value="V-TYPE PROTON ATPASE CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16886">
        <property type="entry name" value="ATP-synt_ab_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="12">
        <location>
            <begin position="22"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="ATPsynthase alpha/beta subunit barrel-sandwich" evidence="13">
        <location>
            <begin position="126"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="11">
        <location>
            <begin position="230"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase A/B type C-terminal" evidence="14">
        <location>
            <begin position="463"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF22919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017309168.1"/>
        </source>
    </evidence>
    <sequence length="617" mass="68339" checksum="C826BCAF8A89D5CB" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PSX2</accession>
    <name>A0A2D0PSX2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tetratricopeptide repeat protein 30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">flr</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Required for polyglutamylation of axonemal tubulin. Plays a role in anterograde intraflagellar transport (IFT), the process by which cilia precursors are transported from the base of the cilium to the site of their incorporation at the tip.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell projection</location>
            <location evidence="1 10">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TTC30/dfy-1/fleer family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017308521.1">
        <property type="nucleotide sequence ID" value="XM_017453032.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PSX2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022843"/>
    <dbReference type="GeneID" id="108256318"/>
    <dbReference type="KEGG" id="ipu:108256318"/>
    <dbReference type="CTD" id="797829"/>
    <dbReference type="OMA" id="CCKHELY"/>
    <dbReference type="OrthoDB" id="10249577at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005879">
        <property type="term" value="C:axonemal microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030992">
        <property type="term" value="C:intraciliary transport particle B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120170">
        <property type="term" value="F:intraciliary transport particle B binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070738">
        <property type="term" value="F:tubulin-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007368">
        <property type="term" value="P:determination of left/right symmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001736">
        <property type="term" value="P:establishment of planar polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036372">
        <property type="term" value="P:opsin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000226">
        <property type="entry name" value="Tetratricopeptide repeat protein 30A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000211">
        <property type="entry name" value="tetratricopeptide repeat protein 30B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039941">
        <property type="entry name" value="TT30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20931">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20931:SF0">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7 10" id="KW-0969">Cilium</keyword>
    <keyword evidence="4 10" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 9 10" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="42"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017308521.1"/>
        </source>
    </evidence>
    <sequence length="651" mass="75201" checksum="FB20CBA36F5FDE12" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PY96</accession>
    <name>A0A2D0PY96_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4 5">Uncharacterized protein si:ch211-142k18.1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4 5" type="primary">si:ch211-142k18.1</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3 4 5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3 4 5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017311006.1">
        <property type="nucleotide sequence ID" value="XM_017455517.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017311007.1">
        <property type="nucleotide sequence ID" value="XM_017455518.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017311008.1">
        <property type="nucleotide sequence ID" value="XM_017455519.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PY96"/>
    <dbReference type="GeneID" id="108257612"/>
    <dbReference type="KEGG" id="ipu:108257612"/>
    <dbReference type="OrthoDB" id="9946098at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013510183" evidence="1">
        <location>
            <begin position="22"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017311006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017311007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017311008.1"/>
        </source>
    </evidence>
    <sequence length="218" mass="24093" checksum="9954970CED8B93E7" modified="2017-12-20" version="1">MEQRWTLCVLLFIATLILGHCQSGDGGDWGSGDMPELLFTTTLTPIPNTVCNDPANPGSTDWTKPLFHVIPDSKADGCSVNFHTSQAMSRRLQVAKEEMAYLKALQHGNQAVMENLIQFVGAEMGDQSYQEIIQENIAGIKENHASCVGVLKKATEELENQLEGADLAGIQKISEESLTFEGMLRATTDIANQLETSSRTLHMQITEQLRKSLKLKRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q4P7</accession>
    <name>A0A2D0Q4P7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">tfdp1b</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">Belongs to the E2F/DP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017313688.1">
        <property type="nucleotide sequence ID" value="XM_017458199.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q4P7"/>
    <dbReference type="GeneID" id="108259062"/>
    <dbReference type="KEGG" id="ipu:108259062"/>
    <dbReference type="CTD" id="393749"/>
    <dbReference type="OrthoDB" id="552115at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14458">
        <property type="entry name" value="DP_DD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000047">
        <property type="entry name" value="Transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.80:FF:000001">
        <property type="entry name" value="Transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.80">
        <property type="entry name" value="Transcription factor DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037241">
        <property type="entry name" value="E2F-DP_heterodim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003316">
        <property type="entry name" value="E2F_WHTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038168">
        <property type="entry name" value="TF_DP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014889">
        <property type="entry name" value="Transc_factor_DP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015648">
        <property type="entry name" value="Transcrpt_fac_DP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548:SF4">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08781">
        <property type="entry name" value="DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02319">
        <property type="entry name" value="WHD_E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009404">
        <property type="entry name" value="Transcription_factor_DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01138">
        <property type="entry name" value="DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01372">
        <property type="entry name" value="E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144074">
        <property type="entry name" value="E2F-DP heterodimerization region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="E2F/DP family winged-helix DNA-binding" evidence="11">
        <location>
            <begin position="129"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor DP C-terminal" evidence="10">
        <location>
            <begin position="218"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="100"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="359"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="113"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR009404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017313688.1"/>
        </source>
    </evidence>
    <sequence length="414" mass="45957" checksum="5CBFF2DDECDF1583" modified="2017-12-20" version="1">MMARDGGLKEASGELKVFTTQNVSPGTGEFPPRNPGVLFVREGAVVSLVTVHPSSVTGVKQIMPKALGGPGVNVLSHVVIGTPQRPSAPAAAALLTSPRTPSAQFLTPQTQARESSPWSSGRSSRRGDKNGKGLRHFSMKVCEKVQKKGVTSYNEVADELVTELSSGDNISPNDAHVYDQKNIRRRVYDALNVLMAMNIISKDKKEIKWIGFPTNSAQECKDLEAERQRRLERIKQKQSQLQELILQQIAFKRLVQRNREAEQKNHRPPPNTVIHLPFIIVNTSKKTVIDCSISNDKFEYLFNFDNMFEIHDDIEVLKRMGMALGLDSGKCTPENLKIARTLIPKALEPYVTDMAQGVHRHSHTNSDNGAVATMASSSNSSYHGSSRDTPASFLEDEEEDEDEDDEDFDDDDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q677</accession>
    <name>A0A2D0Q677_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Melanocortin-2 receptor accessory protein 2A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">mrap2a</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MRAP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017313206.1">
        <property type="nucleotide sequence ID" value="XM_017457717.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q677"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000036231"/>
    <dbReference type="GeneID" id="108258772"/>
    <dbReference type="KEGG" id="ipu:108258772"/>
    <dbReference type="CTD" id="100003330"/>
    <dbReference type="OMA" id="WDYEYYE"/>
    <dbReference type="OrthoDB" id="9904651at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031780">
        <property type="term" value="F:corticotropin hormone receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030545">
        <property type="term" value="F:signaling receptor regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070996">
        <property type="term" value="F:type 1 melanocortin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031781">
        <property type="term" value="F:type 3 melanocortin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031782">
        <property type="term" value="F:type 4 melanocortin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031783">
        <property type="term" value="F:type 5 melanocortin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048589">
        <property type="term" value="P:developmental growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097009">
        <property type="term" value="P:energy homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006112">
        <property type="term" value="P:energy reserve metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106072">
        <property type="term" value="P:negative regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034394">
        <property type="term" value="P:protein localization to cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040008">
        <property type="term" value="P:regulation of growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028111">
        <property type="entry name" value="MRAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28675">
        <property type="entry name" value="MELANOCORTIN-2 RECEPTOR ACCESSORY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28675:SF1">
        <property type="entry name" value="MELANOCORTIN-2 RECEPTOR ACCESSORY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15183">
        <property type="entry name" value="MRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="128"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017313206.1"/>
        </source>
    </evidence>
    <sequence length="219" mass="24697" checksum="EF555A89D6D2F221" modified="2017-12-20" version="1">MPMLQHPNTTAGVSHTDYEWRYEYYDDEEPVSFEGLKAHKYSIVIGFWVGLAVFVIFMFFVLTLLTKTGAPHPESAEPCEDCVHLTSCVEELGRPREPDNTRAGLPRPLLEESHFLFNCYINEEEQAGERSLSQTHRVHTQSSSSESSGQVKTGRLISQSTHLEMMEDNEAAFLAHFNIPNFVNSELSSTLGDDDLLLGEPPIIMESESHSHSTSHIRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QG50</accession>
    <name>A0A2D0QG50_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Reticulon</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rtn4b</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Endoplasmic reticulum membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017316701.1">
        <property type="nucleotide sequence ID" value="XM_017461212.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QG50"/>
    <dbReference type="GeneID" id="108260730"/>
    <dbReference type="KEGG" id="ipu:108260730"/>
    <dbReference type="CTD" id="678586"/>
    <dbReference type="OrthoDB" id="567788at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2480:FF:000001">
        <property type="entry name" value="Reticulon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003388">
        <property type="entry name" value="Reticulon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046964">
        <property type="entry name" value="RTN1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45799">
        <property type="entry name" value="RETICULON-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45799:SF2">
        <property type="entry name" value="RETICULON-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02453">
        <property type="entry name" value="Reticulon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50845">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="777"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="881"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="domain" description="Reticulon" evidence="8">
        <location>
            <begin position="763"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="101"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="222"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="297"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="501"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="580"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="662"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="257"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="298"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="392"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="501"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="515"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="590"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="622"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU210713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017316701.1"/>
        </source>
    </evidence>
    <sequence length="950" mass="104701" checksum="9F428B6198D65003" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QN02</accession>
    <name>A0A2D0QN02_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">GTPase IMAP family member 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Immune-associated nucleotide-binding protein 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC108263508</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Exerts an anti-apoptotic effect in the immune system and is involved in responses to infections.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017319868.1">
        <property type="nucleotide sequence ID" value="XM_017464379.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QN02"/>
    <dbReference type="GeneID" id="108263508"/>
    <dbReference type="KEGG" id="ipu:108263508"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000536">
        <property type="entry name" value="GTPase IMAP family member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF170">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="AIG1-type G" evidence="17">
        <location>
            <begin position="11"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017319868.1"/>
        </source>
    </evidence>
    <sequence length="310" mass="34831" checksum="21C690A85874981D" modified="2017-12-20" version="1">MSAMETEKGAKEHLRLVLVGLQGVGKSAAGNTILGRGEFRSDISSSALTLQTECREGLVCGRSVIVVDTPGLFNSTLSDTEMKQEMEKAVTLCQPGPHAFLIIIQLGRFTEQERHVMDELTELLGSNISLYSMVLFTYGDKLKKKTIDQFVKEDKYLMKLIGKCGGRYHVFNNSDTENKAQVSDFLEKIDTMIKRRKKHPLYVKSARHVQYPWAKILCITLPVVTLLAVTMMYTKKQVPAESQPSSVKEVVSNEVTKKVIESVPVQPEDVLETSARFWQMLHGVDFNWKGLIPIGLGLGAGWWILRKLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QNT8</accession>
    <name>A0A2D0QNT8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA binding protein fox-1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">rbfox1l</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">RNA-binding protein that regulates alternative splicing events.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017320067.1">
        <property type="nucleotide sequence ID" value="XM_017464578.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QNT8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000045"/>
    <dbReference type="GeneID" id="108263593"/>
    <dbReference type="KEGG" id="ipu:108263593"/>
    <dbReference type="CTD" id="407613"/>
    <dbReference type="OrthoDB" id="5382468at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12407">
        <property type="entry name" value="RRM_FOX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000004">
        <property type="entry name" value="RNA binding fox-1 homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025670">
        <property type="entry name" value="Fox-1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034237">
        <property type="entry name" value="FOX1_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017325">
        <property type="entry name" value="RBFOX1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047131">
        <property type="entry name" value="RBFOX1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15597">
        <property type="entry name" value="ATAXIN 2-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15597:SF40">
        <property type="entry name" value="RNA BINDING PROTEIN FOX-1 HOMOLOG 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12414">
        <property type="entry name" value="Fox-1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037932">
        <property type="entry name" value="Ataxin_2_bd_A2BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="145"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="31"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR037932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR037932-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017320067.1"/>
        </source>
    </evidence>
    <sequence length="379" mass="40299" checksum="CABAC200209B8DE2" modified="2017-12-20" version="1">MLSSPAVILQPYGLPVYPQTSCYPSLVQGAPAQEAGPGSSDPALTQVYAAPPSYPPPGQAPPTPAARLPPLDFSSAHSSSDYPDHHQLRVYQSAQHEGAEPIPANNTDEGLAPVTSDPQPLNVPLATGGAGSGSEEEGSGKAQPKRLHVSNIPFRFRDPDLRQMFGQFGKILDVEIIFNERGSKGFGFVTFENAAEADRAREKLNGTIVEGRKIEVNNATARVVTKKPQTPIVNAAGWKINPMMGAMYAPELYTVASFPYPVPTATLAYRGSALRGRGRAVYNTIRSAAAAAATPTAVPAYPGVVYQDGLYGTEVYGGYPAAYRVAQSPSATATATYSDGYGRLYTTDPYHHSVGPTTTYGVSTMASLYRGGYNRFTPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R0Q4</accession>
    <name>A0A2D0R0Q4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA excision repair protein ERCC-6-like 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ercc6l2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017324222.1">
        <property type="nucleotide sequence ID" value="XM_017468733.3"/>
    </dbReference>
    <dbReference type="GeneID" id="108265899"/>
    <dbReference type="KEGG" id="ipu:108265899"/>
    <dbReference type="CTD" id="375748"/>
    <dbReference type="OMA" id="PDSEPCH"/>
    <dbReference type="OrthoDB" id="448448at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18005">
        <property type="entry name" value="DEXHc_ERCC6L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000019">
        <property type="entry name" value="DNA excision repair protein ERCC-6-like 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058052">
        <property type="entry name" value="DEXHc_ERCC6L2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029256">
        <property type="entry name" value="Heliccase-ass-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057931">
        <property type="entry name" value="RHH_ERCC6L2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25806">
        <property type="entry name" value="RHH_ERCC6L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14773">
        <property type="entry name" value="VIGSSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="725"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="795"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1066"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1124"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1198"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="800"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1066"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1133"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1151"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1198"/>
            <end position="1208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017324222.1"/>
        </source>
    </evidence>
    <sequence length="1283" mass="144706" checksum="6196A8E4FAF20062" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R1L1</accession>
    <name>A0A2D0R1L1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sulfotransferase</fullName>
            <ecNumber evidence="6">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC108265623</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="6"/>
    <dbReference type="RefSeq" id="XP_017323660.1">
        <property type="nucleotide sequence ID" value="XM_017468171.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R1L1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033276"/>
    <dbReference type="GeneID" id="108265623"/>
    <dbReference type="KEGG" id="ipu:108265623"/>
    <dbReference type="OrthoDB" id="205623at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006584">
        <property type="term" value="P:catecholamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000433">
        <property type="entry name" value="Estrogen sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0128">Catecholamine metabolism</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="7">
        <location>
            <begin position="43"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017323660.1"/>
        </source>
    </evidence>
    <sequence length="301" mass="35433" checksum="CBDC343DAAD61973" modified="2017-12-20" version="1">MEGVDLSFLKSISRPEMFEFEGISMVHYFTDNWENVQNFQARPDDILIATYPKAGTTWVSYILELLYFGNVAPERQTSMPIYIRVPFLESQFPGIPSGVELADNLPTTPRLIKTHLPVQLVPKSFFEQNCKIVYVARNAKDNVVSYFHFERMNMGQPDPGNWNNFLHSFMEGKKVFGPWYDHVTGYWEKKQTYSNLHYMFFEDMVEDMGCEMERLCSFLGLSTPVEERERIIKSTHFDTMKQNKMTNYSTIPIMDFTISPFMRKGKVGDWKNHFTVAQNEQFDEHYKQKMKNTTVQFRTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R306</accession>
    <name>A0A2D0R306_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Semaphorin-5A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Semaphorin-F</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20 21 22" type="primary">sema5ba</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20 21 22" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20 21 22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Binds PLXNB3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017324988.2">
        <property type="nucleotide sequence ID" value="XM_017469499.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017324989.2">
        <property type="nucleotide sequence ID" value="XM_017469500.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017324990.2">
        <property type="nucleotide sequence ID" value="XM_017469501.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R306"/>
    <dbReference type="GeneID" id="108266347"/>
    <dbReference type="KEGG" id="ipu:108266347"/>
    <dbReference type="CTD" id="556471"/>
    <dbReference type="GeneTree" id="ENSGT00940000156712"/>
    <dbReference type="OrthoDB" id="9988752at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:positive regulation of cell migration"/>
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    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="semaphorin-5A isoform X1"/>
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        <property type="entry name" value="semaphorin-5B isoform X1"/>
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        <property type="entry name" value="Thrombospondin 1"/>
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        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="6"/>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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        <property type="entry name" value="PSI"/>
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        <property type="entry name" value="Semap_dom"/>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SEMAPHORIN-5B"/>
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        <property type="entry name" value="TSP1_2"/>
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        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01705">
        <property type="entry name" value="TSP1REPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
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    </dbReference>
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        <property type="entry name" value="Plexin repeat"/>
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    </dbReference>
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        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="TSP-1 type 1 repeat"/>
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        <property type="entry name" value="SEMA"/>
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    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044573413" description="Semaphorin-5A" evidence="17">
        <location>
            <begin position="31"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="977"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="18">
        <location>
            <begin position="53"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00056685"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00062302"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00074104"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00083067"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017324988.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017324989.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017324990.2"/>
        </source>
    </evidence>
    <sequence length="1097" mass="122444" checksum="29D59219AF613269" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R4Q7</accession>
    <name>A0A2D0R4Q7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ddx3xa</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="RefSeq" id="XP_017325031.1">
        <property type="nucleotide sequence ID" value="XM_017469542.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A2D0R4Q7"/>
    <dbReference type="GeneID" id="108266363"/>
    <dbReference type="KEGG" id="ipu:108266363"/>
    <dbReference type="CTD" id="566947"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18051">
        <property type="entry name" value="DEADc_DDX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000160">
        <property type="entry name" value="ATP-dependent RNA helicase DDX3X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10 16" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="14">
        <location>
            <begin position="265"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="296"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="503"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="18"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="171"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="711"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="9">
        <location>
            <begin position="265"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="723"/>
            <end position="733"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017325031.1"/>
        </source>
    </evidence>
    <sequence length="767" mass="84370" checksum="64B31A2103E26C06" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R755</accession>
    <name>A0A2D0R755_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Stress-associated endoplasmic reticulum protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">serp2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Interacts with target proteins during their translocation into the lumen of the endoplasmic reticulum. Protects unfolded target proteins against degradation during ER stress. May facilitate glycosylation of target proteins after termination of ER stress. May modulate the use of N-glycosylation sites on target proteins.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Interacts with target proteins during translocation into the lumen of the endoplasmic reticulum. Protects unfolded target proteins against degradation and facilitate correct glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with SEC61B, SEC61A1 and the SEC61 complex. Interacts with CANX.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the RAMP4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017325886.1">
        <property type="nucleotide sequence ID" value="XM_017470397.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R755"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033116"/>
    <dbReference type="GeneID" id="108266728"/>
    <dbReference type="KEGG" id="ipu:108266728"/>
    <dbReference type="CTD" id="387923"/>
    <dbReference type="OMA" id="ATKFITQ"/>
    <dbReference type="OrthoDB" id="16679at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010580">
        <property type="entry name" value="ER_stress-assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15601">
        <property type="entry name" value="STRESS ASSOCIATED ENDOPLASMIC RETICULUM PROTEIN SERP1/RAMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15601:SF20">
        <property type="entry name" value="STRESS-ASSOCIATED ENDOPLASMIC RETICULUM PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06624">
        <property type="entry name" value="RAMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0834">Unfolded protein response</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017325886.1"/>
        </source>
    </evidence>
    <sequence length="65" mass="7413" checksum="4A689CEF04336C06" modified="2017-12-20" version="1">MVAKQRIRMANEKHSKNITQRGNVAKTLRPQEEKYPVGPWLLALFVFVVCGSAIFQIIQSIRMGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R811</accession>
    <name>A0A2D0R811_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein crumbs homolog 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC108267236</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017326717.2">
        <property type="nucleotide sequence ID" value="XM_017471228.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R811"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000017674"/>
    <dbReference type="GeneID" id="108267236"/>
    <dbReference type="KEGG" id="ipu:108267236"/>
    <dbReference type="OrthoDB" id="283575at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000348">
        <property type="entry name" value="Crumbs 1, cell polarity complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000282">
        <property type="entry name" value="Crumbs cell polarity complex component 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000252">
        <property type="entry name" value="Crumbs homolog 1 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000007">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000279">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000143">
        <property type="entry name" value="Protein crumbs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916:SF14">
        <property type="entry name" value="CRUMBS 1, CELL POLARITY COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE VIC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5037780395" evidence="6">
        <location>
            <begin position="19"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="23"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="67"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="7">
        <location>
            <begin position="109"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="293"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="7">
        <location>
            <begin position="336"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="510"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="7">
        <location>
            <begin position="573"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="762"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="800"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="837"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="892"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="932"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
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        <source>
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        </source>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017326717.2"/>
        </source>
    </evidence>
    <sequence length="1011" mass="112703" checksum="619EDB989E17DEEA" modified="2023-02-22" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RFM6</accession>
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            <fullName evidence="12">Uncharacterized protein LOC108268729</fullName>
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        <name evidence="12" type="primary">LOC108268729</name>
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        <name type="scientific">Ictalurus punctatus</name>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
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                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
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                <person name="Liu H."/>
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                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
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                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
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                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
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                <person name="Sonstegard T."/>
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                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
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                <person name="Kirby M.L."/>
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                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="3">Blood</tissue>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(3S)-3-hydroxy-3-methylglutaryl-CoA = acetoacetate + acetyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:24404"/>
            <dbReference type="ChEBI" id="CHEBI:13705"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="4.1.3.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate metabolism; (S)-3-hydroxy-3-methylglutaryl-CoA degradation; acetoacetate from (S)-3-hydroxy-3-methylglutaryl-CoA: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HMG-CoA lyase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.4" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017330839.1">
        <property type="nucleotide sequence ID" value="XM_017475350.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RJY2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000023129"/>
    <dbReference type="GeneID" id="108269453"/>
    <dbReference type="KEGG" id="ipu:108269453"/>
    <dbReference type="CTD" id="3155"/>
    <dbReference type="OMA" id="FQMRNTH"/>
    <dbReference type="OrthoDB" id="1905920at2759"/>
    <dbReference type="UniPathway" id="UPA00896">
        <property type="reaction ID" value="UER00863"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004419">
        <property type="term" value="F:hydroxymethylglutaryl-CoA lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046951">
        <property type="term" value="P:ketone body biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006552">
        <property type="term" value="P:L-leucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07938">
        <property type="entry name" value="DRE_TIM_HMGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000038">
        <property type="entry name" value="Hydroxymethylglutaryl-CoA lyase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043594">
        <property type="entry name" value="HMGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004283">
        <property type="entry name" value="PRK05692.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42738">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42738:SF18">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA LYASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="7">
        <location>
            <begin position="37"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017330839.1"/>
        </source>
    </evidence>
    <sequence length="329" mass="34909" checksum="FBF298C96A974957" modified="2017-12-20" version="1">MAAVMMRIVQASFSGRCCYSVAKSVSLCATRPLPEKVKIVEVGPRDGLQNEKTIVPAEVKIRLIDMLSEAGLPVIEATSFVSPKWVPQMADREEVMQGINRKPGVNYPVLTPNLKGYRAAVTAGAKEVAIFGAASELFSKKNINCTVEESLQRFEEVTSAAKQEGVPVRGYVSCVLGCPYEGNVSPMKVADVAKRLYAMGCYEISLGDTIGVGTPGGMSQMLEAVMKEVPVDALAVHCHDTYGQALANILVALQNGVSVVDSSVSGLGGCPYAQGASGNVATEDVVYMLHGLGIHTGVDLQKLMDAGSFICRSLNRRTSSKVAQASCKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RLH9</accession>
    <name>A0A2D0RLH9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16 17" type="primary">gja1a</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16 17" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16 17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 9">Cell membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017330805.1">
        <property type="nucleotide sequence ID" value="XM_017475316.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017330806.1">
        <property type="nucleotide sequence ID" value="XM_017475317.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RLH9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008102"/>
    <dbReference type="GeneID" id="108269439"/>
    <dbReference type="KEGG" id="ipu:108269439"/>
    <dbReference type="CTD" id="565638"/>
    <dbReference type="OrthoDB" id="8773830at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010644">
        <property type="term" value="P:cell communication by electrical coupling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.80:FF:000001">
        <property type="entry name" value="Gap junction alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013124">
        <property type="entry name" value="Connexin43_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034634">
        <property type="entry name" value="Connexin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF33">
        <property type="entry name" value="GAP JUNCTION ALPHA-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03508">
        <property type="entry name" value="Connexin43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118220">
        <property type="entry name" value="Connexin43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00407">
        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0303">Gap junction</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="209"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="13">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="14">
        <location>
            <begin position="165"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="338"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="338"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017330805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017330806.1"/>
        </source>
    </evidence>
    <sequence length="358" mass="41268" checksum="67682A267A36F02E" modified="2017-12-20" version="1">MGDWSTLAKLLDKVQVYSTAGGKLWLSVLFIFRILVLGTAVESAWGDEQSAFECNTQQPGCENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLGHVLFLMHKDEKLKKKEDRLRKAEREGSNVHARLHKIQAKKFKYGLEEPGKIKMRGALFYTYIVSVVLKSVFEVAFLLIQWYLYGFRLSAVYTCERFPCPHKVDCFLSRPTEKTVFIMFMLVVSLISLALNVFEFFAAIFKRMKDHFREAEKLLDMESTIRPCRGEDMSGYGYHCSAPASNLGYNLDTADKSNSSDNFDKQANEQNWTNYSTEQNQLGQRDYCPSQTFVYPQKMPLGKDLLLLKQLDPRPQSRSSSRARPDDLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RNE1</accession>
    <name>A0A2D0RNE1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 15">BRISC and BRCA1-A complex member 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17 18" type="primary">babam2</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17 18">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">May play a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. May also act as a death receptor-associated anti-apoptotic protein, which inhibits the mitochondrial apoptotic pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the ARISC complex. Component of the BRCA1-A complex. Component of the BRISC complex. Binds polyubiquitin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
        <text evidence="15">Localizes at sites of DNA damage at double-strand breaks (DSBs).</text>
    </comment>
    <comment type="domain">
        <text evidence="15">Contains 2 ubiquitin-conjugating enzyme family-like (UEV-like) regions. These regions lack the critical Cys residues required for ubiquitination but retain the ability to bind ubiquitin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14 15">Belongs to the BABAM2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017332021.1">
        <property type="nucleotide sequence ID" value="XM_017476532.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017332022.1">
        <property type="nucleotide sequence ID" value="XM_017476533.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RNE1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000014945"/>
    <dbReference type="GeneID" id="108270148"/>
    <dbReference type="KEGG" id="ipu:108270148"/>
    <dbReference type="CTD" id="9577"/>
    <dbReference type="OMA" id="AGSTWRH"/>
    <dbReference type="OrthoDB" id="538811at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070531">
        <property type="term" value="C:BRCA1-A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070552">
        <property type="term" value="C:BRISC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045739">
        <property type="term" value="P:positive regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23664">
        <property type="entry name" value="BRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010358">
        <property type="entry name" value="BRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15189">
        <property type="entry name" value="BRISC AND BRCA1-A COMPLEX MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15189:SF7">
        <property type="entry name" value="BRISC AND BRCA1-A COMPLEX MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06113">
        <property type="entry name" value="BRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="13 15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 15" id="KW-0132">Cell division</keyword>
    <keyword evidence="10 15" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 15" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017332021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017332022.1"/>
        </source>
    </evidence>
    <sequence length="386" mass="43814" checksum="DD888434F37D5AF5" modified="2017-12-20" version="1">MNSLSPELALSRISPELRPLLCTVVRNGRVGLDSTNCLRITDLKSGCTSLTPGPCCDRFKLHIPYAGETLKWDIIFNARDPELPPDFIFGEDAEFLPEPSELPHLVAWDAGDTECLLQLVKELLQQYHQYQCQRLRDSSRLLFEYDSLLEDPNYGRSMEIYAGRKNSWTGEFSARFLLKLPVDFSNIPIYLLKDTALDPGEDVALLSVSFEDAEATQVFPKLFLSPSIEHALGGPSALHIPAFPSGGCLIDYVPQVCQLLTNKVQYVIQGYHRRREYIAAFLSHFGMGVVEYDAVGFTKLTLLLMWKDFCFLVHVDLPLYFPRDQPTLTFQSIYHFSSSGQLYSQVQKSYPYSPRWDGNEMAKRAKAYFKSFIPQFQEGAFANGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RRA2</accession>
    <name>A0A2D0RRA2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Monoacylglycerol lipase ABHD2</fullName>
            <ecNumber evidence="5">3.1.1.23</ecNumber>
            <ecNumber evidence="23">3.1.1.6</ecNumber>
            <ecNumber evidence="4">3.1.1.79</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">2-arachidonoylglycerol hydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">Acetylesterase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Triacylglycerol lipase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="primary">abhd2a</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="29">SDA103</strain>
        </source>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="30">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="22">Progesterone-dependent acylglycerol lipase that catalyzes hydrolysis of endocannabinoid arachidonoylglycerol (AG) from cell membrane. Acts as a progesterone receptor: progesterone-binding activates the acylglycerol lipase activity, mediating degradation of 1-arachidonoylglycerol (1AG) and 2-arachidonoylglycerol (2AG) to glycerol and arachidonic acid (AA). Also displays an ester hydrolase activity against acetyl ester, butanoate ester and hexadecanoate ester. Plays a key role in sperm capacitation in response to progesterone by mediating degradation of 2AG, an inhibitor of the sperm calcium channel CatSper, leading to calcium influx via CatSper and sperm activation. May also play a role in smooth muscle cells migration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + H2O = glycerol + (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:26132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:52392"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:26133"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolyzes glycerol monoesters of long-chain fatty acids.</text>
            <dbReference type="EC" id="3.1.1.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a butanoate ester + H2O = an aliphatic alcohol + butanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47348"/>
            <dbReference type="ChEBI" id="CHEBI:2571"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17968"/>
            <dbReference type="ChEBI" id="CHEBI:50477"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:47349"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.79"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:12045"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>an acetyl ester + H2O = an aliphatic alcohol + acetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12957"/>
            <dbReference type="ChEBI" id="CHEBI:2571"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:47622"/>
            <dbReference type="EC" id="3.1.1.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:12958"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>hexadecanoate ester + H2O = an aliphatic alcohol + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47392"/>
            <dbReference type="ChEBI" id="CHEBI:2571"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25835"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:47393"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AB hydrolase superfamily. AB hydrolase 4 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.23" evidence="5"/>
    <dbReference type="EC" id="3.1.1.6" evidence="23"/>
    <dbReference type="EC" id="3.1.1.79" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017333032.1">
        <property type="nucleotide sequence ID" value="XM_017477543.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RRA2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034133"/>
    <dbReference type="GeneID" id="108270704"/>
    <dbReference type="KEGG" id="ipu:108270704"/>
    <dbReference type="CTD" id="393888"/>
    <dbReference type="OMA" id="HCTGEDV"/>
    <dbReference type="OrthoDB" id="5954035at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036126">
        <property type="term" value="C:sperm flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097524">
        <property type="term" value="C:sperm plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008126">
        <property type="term" value="F:acetylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046464">
        <property type="term" value="P:acylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051792">
        <property type="term" value="P:medium-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051793">
        <property type="term" value="P:medium-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048240">
        <property type="term" value="P:sperm capacitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043401">
        <property type="term" value="P:steroid hormone receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000053">
        <property type="entry name" value="Abhydrolase domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050960">
        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012020">
        <property type="entry name" value="ABHD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF45">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE ABHD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005211">
        <property type="entry name" value="Ab_hydro_YheT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="28">
        <location>
            <begin position="132"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="27">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="27">
        <location>
            <position position="347"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="27">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00033629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00050185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00050565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00051791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00060066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00067090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00071478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00075295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00076966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR005211-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="RefSeq" id="XP_017333032.1"/>
        </source>
    </evidence>
    <sequence length="433" mass="48377" checksum="2046284E996E39C2" modified="2017-12-20" version="1">MGTHESEVYTVAPEMPAMFDGMKLAAVATVLYIIVRCLNLKSPTAPPDITYQDTPLSRFLLKSCPLLTKEYIPPLLWGKSGHLQTALYGKLGRVSSPHPTGLRKYLPMQDGATATFDLFEPLADHQSGADVTMVICPGIGNHSEKHYIRTFVDYSQKQGYRCAVLNHLGALPNIELTSPRMFTYGCTWEFAAMVGYIKKTYPQSQLIVVGFSLGGNIVCKFLGENHANQERVLCCVSMCQGYSALRAQETFLQWDQCRRFYNFLMADNMKKIILSHRDSLFGGGSPKMVDADLRRLYTATSLMQIDDNVMRKFHGHDSLKEYYEKESCVHYIHNVNVPLLLVNSADDPLVHQSLLTIPRTLAEKKENVIFALTLHGGHLGFFEGAVLFPQPLTWMDKVIVGYANAICQWQKQKPACQSQDAQASGGTCLNTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RRW9</accession>
    <name>A0A2D0RRW9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ELAV-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">elavl1b</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the RRM elav family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017333246.1">
        <property type="nucleotide sequence ID" value="XM_017477757.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017333248.1">
        <property type="nucleotide sequence ID" value="XM_017477759.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RRW9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000023305"/>
    <dbReference type="GeneID" id="108270814"/>
    <dbReference type="KEGG" id="ipu:108270814"/>
    <dbReference type="CTD" id="30065"/>
    <dbReference type="GeneTree" id="ENSGT00940000155528"/>
    <dbReference type="OMA" id="QANTCIS"/>
    <dbReference type="OrthoDB" id="266020at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12769">
        <property type="entry name" value="RRM1_HuR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12653">
        <property type="entry name" value="RRM3_HuR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000006">
        <property type="entry name" value="ELAV-like 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000005">
        <property type="entry name" value="ELAV-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000215">
        <property type="entry name" value="ELAV-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006548">
        <property type="entry name" value="ELAD_HU_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002343">
        <property type="entry name" value="Hud_Sxl_RNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01661">
        <property type="entry name" value="ELAV_HUD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00961">
        <property type="entry name" value="HUDSXLRNA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="57"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="143"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="284"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017333246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017333248.1"/>
        </source>
    </evidence>
    <sequence length="366" mass="40517" checksum="AA01E0EEEE99AEFE" modified="2017-12-20" version="1">MAVRRGHIRYLKVCEVQSQGNDRDLQQLGKGTSMNDAYDLPNGFESHLGDEAKDAKTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKMGGHSLGYGFVNYINPSDAERAISTLNGLRLQSKTIKVSYARPSSDTIKDANLYISGLPKTMTQKDVEDLFTQYGRIINSRVLVDQASGNDCLSRGVAFIRFDKRIEAEDAIKGLNGQKPPGASEPITVKFAANPNQAKNSQLLSQFYNTQSRRFGGPVHHQAQRFRFSPMSVDHMSGVSSMNVAGSSTSGWCIFIYNLGQDSDEGLLWQMFGPFGAVTNVKVIRDFNTNKCRGFGFVTMTNYEEAAMAIASLNGYRLGDKVLQVSFKTSKSHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RTA4</accession>
    <name>A0A2D0RTA4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 17">Dihydroorotate dehydrogenase (quinone), mitochondrial</fullName>
            <shortName evidence="17">DHOdehase</shortName>
            <ecNumber evidence="4 17">1.3.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">dhodh</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor. Required for UMP biosynthesis via de novo pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>(S)-dihydroorotate + a quinone = orotate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:30187"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="17">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 17">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Mitochondrion inner membrane</location>
            <topology evidence="1 17">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.2" evidence="4 17"/>
    <dbReference type="RefSeq" id="XP_017333406.1">
        <property type="nucleotide sequence ID" value="XM_017477917.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RTA4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000017084"/>
    <dbReference type="GeneID" id="108270907"/>
    <dbReference type="KEGG" id="ipu:108270907"/>
    <dbReference type="CTD" id="1723"/>
    <dbReference type="OMA" id="ERIKMGA"/>
    <dbReference type="OrthoDB" id="14784at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00946"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106430">
        <property type="term" value="F:dihydroorotate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04738">
        <property type="entry name" value="DHOD_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000066">
        <property type="entry name" value="Dihydroorotate dehydrogenase (quinone), mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00225">
        <property type="entry name" value="DHO_dh_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050074">
        <property type="entry name" value="DHO_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005719">
        <property type="entry name" value="Dihydroorotate_DH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003645">
        <property type="entry name" value="PRK05286.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003652">
        <property type="entry name" value="PRK05286.2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01036">
        <property type="entry name" value="pyrD_sub2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109:SF4">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00911">
        <property type="entry name" value="DHODEHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 17" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 17" id="KW-0288">FMN</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9 17" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Dihydroorotate dehydrogenase catalytic" evidence="19">
        <location>
            <begin position="77"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="386"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="396"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017333406.1"/>
        </source>
    </evidence>
    <sequence length="409" mass="43971" checksum="EB4691BC958D505D" modified="2017-12-20" version="1">MAGQLKERLKDAVKILGCGSTLFLGYLTATGDERFYATTLMPVLQRLVGAETAHVMAVRLLSLGLVPRSRYQDPASLEVNVMGRRFGNPVGIAAGFDKHGEAVDALYRLGFGFVEVGTVTPKPQEGNPKPRVFRLEADQAVINRYGFNSCGVLAVQERLKARQGIQSELTKAGKPLGINLGKNKLSTDAVADYLEGVRTLGPLADYLVVNVSSPNTPGLRDLQGKKELRHLLDKVLKARDTLQGATRPPVMVKIAPDLSQQDKQDIAEVITELGVDGLIVSNTTVSRPETLQDHNRNEAGGLSGQPLKERSTRTVREMYTLTKGKVPIVGVGGVASGQDALDKIRAGASLVQLYTALVYQGPPVVTKIKRELHELLTEQGFSSVSEAVGADHRTSDGMTSSHTEGATVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RU54</accession>
    <name>A0A2D0RU54_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ankyrin repeat and death domain-containing protein 1A isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ankdd1a</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017334029.1">
        <property type="nucleotide sequence ID" value="XM_017478540.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RU54"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034260"/>
    <dbReference type="GeneID" id="108271176"/>
    <dbReference type="KEGG" id="ipu:108271176"/>
    <dbReference type="CTD" id="348094"/>
    <dbReference type="OMA" id="RFYKWEQ"/>
    <dbReference type="OrthoDB" id="448455at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="47"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="80"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="148"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="181"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="214"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="247"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="280"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="313"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Death" evidence="4">
        <location>
            <begin position="420"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017334029.1"/>
        </source>
    </evidence>
    <sequence length="507" mass="56627" checksum="6FDA961493AA576B" modified="2017-12-20" version="1">MEDGLASEDDILVQSEKVFHNAAKRNDTGKMLELIRKGVDIKAKNKADRKALHWAAGAGGEDALRLLLEHDTALDDADSFGMTALLLAAWFGHLKILQILVSSGAKLSCENKKGLSLLHCAAQRGHIRILEYIMEDLEDIGLDKVDKSGKTAFHLAAEHGQLEVTEYLIGMGCAHDLKDKEENTALHLAAKHGHTEVLKKILETGENINEKNIDGLTALHLSVDGGHYDCVQLLLEAHCNVNELTNRNLTALHFAVQHGFEREVRLLVEAGVSVDSVDTNHTTALHLAVFNNYPEIVKILIDAECDLDVFDERLQTALHIAAEHGRQIIAELILIAGVNLKLLDKQGKTSLDIAARSNHVNVVDMIIKADRFYKWEQDSVNCSESLVGRNVTFRQDHQQETQHIRSILWKLATKHLKAGEWKTLAQHWHFTDAHIRAIEHEWTGTKSYKDHGHRMLLIWLHGVLIAGQNPIKCLYEGLMGISRMDLAESMRQQANADPNTSKKCTTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RVW9</accession>
    <name>A0A2D0RVW9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Interferon regulatory factor 2-binding protein 2-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">irf2bp2b</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Acts as a transcriptional repressor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IRF2BP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017334105.1">
        <property type="nucleotide sequence ID" value="XM_017478616.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RVW9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032608"/>
    <dbReference type="GeneID" id="108271215"/>
    <dbReference type="KEGG" id="ipu:108271215"/>
    <dbReference type="CTD" id="406614"/>
    <dbReference type="OMA" id="HPGMEQV"/>
    <dbReference type="OrthoDB" id="45007at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16716">
        <property type="entry name" value="vRING-HC_IRF2BP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1580:FF:000001">
        <property type="entry name" value="interferon regulatory factor 2-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1580">
        <property type="entry name" value="Interferon regulatory factor 2-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044882">
        <property type="entry name" value="I2BP1/2_C3HC4-RING_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058682">
        <property type="entry name" value="IRF-2BP1/2-like_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022750">
        <property type="entry name" value="IRF-2BP1_2-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057414">
        <property type="entry name" value="Zf-C3HC4_IRF-2BP1_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10816:SF18">
        <property type="entry name" value="INTERFERON REGULATORY FACTOR 2-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10816">
        <property type="entry name" value="MYELIN TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11261">
        <property type="entry name" value="IRF-2BP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25457">
        <property type="entry name" value="IRF-2BP1_2_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25454">
        <property type="entry name" value="zf-C3HC4_IRF-2BP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Interferon regulatory factor 2-binding protein 1/2-like zinc finger" evidence="12">
        <location>
            <begin position="10"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="IRF-2BP1/2-like middle" evidence="14">
        <location>
            <begin position="191"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Interferon regulatory factor 2-binding protein 1/2-like C3HC4 zinc finger" evidence="13">
        <location>
            <begin position="434"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="61"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="176"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="242"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="346"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="400"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="186"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="361"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00059947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF11261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF25454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF25457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017334105.1"/>
        </source>
    </evidence>
    <sequence length="517" mass="54625" checksum="270532364AAD9AFF" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RXK4</accession>
    <name>A0A2D0RXK4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glucose-fructose oxidoreductase domain-containing protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">gfod1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfo/Idh/MocA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017334690.1">
        <property type="nucleotide sequence ID" value="XM_017479201.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RXK4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000037175"/>
    <dbReference type="GeneID" id="108271555"/>
    <dbReference type="KEGG" id="ipu:108271555"/>
    <dbReference type="CTD" id="54438"/>
    <dbReference type="OMA" id="NQKDYVH"/>
    <dbReference type="OrthoDB" id="446809at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050463">
        <property type="entry name" value="Gfo/Idh/MocA_oxidrdct_glycsds"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055170">
        <property type="entry name" value="GFO_IDH_MocA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43818">
        <property type="entry name" value="BCDNA.GH03377"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43818:SF2">
        <property type="entry name" value="GLUCOSE-FRUCTOSE OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22725">
        <property type="entry name" value="GFO_IDH_MocA_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="2">
        <location>
            <begin position="5"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="GFO/IDH/MocA-like oxidoreductase" evidence="3">
        <location>
            <begin position="128"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017334690.1"/>
        </source>
    </evidence>
    <sequence length="391" mass="43281" checksum="B3C5F199E71EB8BD" modified="2017-12-20" version="1">MLPGVGVFGTSLTARVIIPLLKSEGFSVEALWGRTQEEAEELAREMDVPFYTSRIDEVLLHQDVDLVCINLPPPLTRQIAVKTLGIGKNVICDRTATPLDAFRMMSAAQYYPKLLSIMGNVLRFLPAFVRMKELLEEGYVGELLVCEAQVHGGSLLGKKYNWSCDDLMGGGGLHSVGTYIIDLLTFLTSRRAIKVHGFLKTFIKQTDHIKGIRQITSDDFCTFQMVLEGGACCTVTLNFNVPGEFRQEVVVVGTAGRLTVSGTDLYGQKNSGMADRELLLKDSTPLGNASLPDKAFSDIPSPYLTGTICMVQAIRQAFQGQEDRRTWDGRPLTMAATFEDCLYALCVVDTIKKSNQCGEWQNIVVMTEEPEVSPAYLISEAMRRSRMSLYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RXM6</accession>
    <name>A0A2D0RXM6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcriptional enhancer factor TEF-5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">tead3b</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017335252.1">
        <property type="nucleotide sequence ID" value="XM_017479763.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017335253.1">
        <property type="nucleotide sequence ID" value="XM_017479764.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RXM6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000037043"/>
    <dbReference type="GeneID" id="108271874"/>
    <dbReference type="KEGG" id="ipu:108271874"/>
    <dbReference type="CTD" id="794293"/>
    <dbReference type="GeneTree" id="ENSGT00950000182956"/>
    <dbReference type="OMA" id="PKFADEP"/>
    <dbReference type="OrthoDB" id="10006572at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048568">
        <property type="term" value="P:embryonic organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035329">
        <property type="term" value="P:hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.50.80:FF:000001">
        <property type="entry name" value="Transcriptional enhancer factor TEF-1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.40">
        <property type="entry name" value="TEA/ATTS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000818">
        <property type="entry name" value="TEA/ATTS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038096">
        <property type="entry name" value="TEA/ATTS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050937">
        <property type="entry name" value="TEC1_TEAD_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027253">
        <property type="entry name" value="TEF-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016361">
        <property type="entry name" value="TEF_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041086">
        <property type="entry name" value="YBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834">
        <property type="entry name" value="TRANSCRIPTIONAL ENHANCER FACTOR TEF RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834:SF7">
        <property type="entry name" value="TRANSCRIPTIONAL ENHANCER FACTOR TEF-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01285">
        <property type="entry name" value="TEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17725">
        <property type="entry name" value="YBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002603">
        <property type="entry name" value="TEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500720">
        <property type="entry name" value="TEF-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00065">
        <property type="entry name" value="TEADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00426">
        <property type="entry name" value="TEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00554">
        <property type="entry name" value="TEA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51088">
        <property type="entry name" value="TEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TEA" evidence="8">
        <location>
            <begin position="2"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="TEA" evidence="7">
        <location>
            <begin position="2"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017335252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017335253.1"/>
        </source>
    </evidence>
    <sequence length="427" mass="48350" checksum="5AD314F78CCA7D35" modified="2017-12-20" version="1">MDSDAEGVWSPDIEQSFQEALAIYPPCGRRKIILSDEGKMYGRNELIARYIKLRTGKTRTRKQVSSHIQVLARKKVREFQAGIKVSSHLQVLARRKSREIQSKLKAMNLDQASKDKALQNMASLSSAQIVSPNLIKSQLPPLPQPPYAPPPRFWPSPIPGQPGTSQDIKPFVHTYTNLSGPVPPPISAYEPLAPPLPPAATAVPVWQDRTIASSKLRMLEYSAFMEVPRDQDTYSKHLFVHIGQTNPSYSDPLLEAVDIRQIYDKFPEKKGGLKELYEKGPQNAFFLVKFWADLNSSDMQDGPGSFYGVSSQYCSTENMTITVSTKVCSFGKQVVEKVETEYARVEGGKCVYRIHRSPMCEYMINFIHKLKHLPEKYMMNSVLENFTILQVVTNRETQETLLCIAFVFEVSTSEHGAQYHVYRLIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RY44</accession>
    <name>A0A2D0RY44_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10 11">RING finger protein 223</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10 11" type="primary">LOC108271944</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9 10 11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10 11">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017335422.1">
        <property type="nucleotide sequence ID" value="XM_017479933.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017335423.1">
        <property type="nucleotide sequence ID" value="XM_017479934.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053539837.1">
        <property type="nucleotide sequence ID" value="XM_053683862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RY44"/>
    <dbReference type="GeneID" id="108271944"/>
    <dbReference type="KEGG" id="ipu:108271944"/>
    <dbReference type="GeneTree" id="ENSGT00940000163259"/>
    <dbReference type="OrthoDB" id="252722at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16556">
        <property type="entry name" value="RING-HC_RNF183-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051435">
        <property type="entry name" value="RING_finger_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22791:SF4">
        <property type="entry name" value="RING FINGER PROTEIN 223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22791">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="46"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017335422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017335423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053539837.1"/>
        </source>
    </evidence>
    <sequence length="234" mass="26187" checksum="85CEE5F766861D47" modified="2017-12-20" version="1">MPICIEDNKMQEPEVLPTTMSNATSDTPETSLPKDADQVWDTAPECSICFTAYDNTFKTPKVLLCNHTFCLECLSRFVAVLPEQKGTQITCPLCRQPTSVPENGPPALATSLEVLGQLPSHQQQEEHVWLDGKRLCYTNPQTPSDVCIDIGGNKQQNENRQEETETNRSILGSCTGLFRRWQRLLIFLLVLAILLIIVIWPIQCIFSRGSTAGCFERQDEHVTTFPTASTSKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S203</accession>
    <name>A0A2D0S203_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6 7">Alpha-taxilin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6 7 8" type="primary">txlna</name>
    </gene>
    <organism evidence="4 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5 6 7" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5 6 7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the taxilin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017336777.1">
        <property type="nucleotide sequence ID" value="XM_017481288.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017336778.1">
        <property type="nucleotide sequence ID" value="XM_017481289.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017336779.1">
        <property type="nucleotide sequence ID" value="XM_017481290.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017336781.1">
        <property type="nucleotide sequence ID" value="XM_017481292.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S203"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021155"/>
    <dbReference type="GeneID" id="108272687"/>
    <dbReference type="KEGG" id="ipu:108272687"/>
    <dbReference type="CTD" id="200081"/>
    <dbReference type="OMA" id="VTEAPCC"/>
    <dbReference type="OrthoDB" id="425555at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026183">
        <property type="entry name" value="Taxilin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16127:SF12">
        <property type="entry name" value="ALPHA-TAXILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16127">
        <property type="entry name" value="TAXILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09728">
        <property type="entry name" value="Taxilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="83"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="454"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="160"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="37"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="514"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017336777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017336778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017336779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017336781.1"/>
        </source>
    </evidence>
    <sequence length="524" mass="59474" checksum="90E27779389E9C6D" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S4K7</accession>
    <name>A0A2D0S4K7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC108272854</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108272854</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017337156.1">
        <property type="nucleotide sequence ID" value="XM_017481667.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S4K7"/>
    <dbReference type="GeneID" id="108272854"/>
    <dbReference type="KEGG" id="ipu:108272854"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017337156.1"/>
        </source>
    </evidence>
    <sequence length="150" mass="16424" checksum="358096536CDD4D09" modified="2017-12-20" version="1">MGERQFFLSPLLEGLDLDLNTCYQLAHLTADITMLLEVLHTPSENVGFPPLLGYRIRPANVCVNASTQTDSLPAVPAVQSSNYQTDEDMAFSDLGSDVSPNFSPVSPEYTPPYTSAGYHSSPGHTPFYQPFSPYRSSLPDYSPTSPMNSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S550</accession>
    <name>A0A2D0S550_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Membrane-spanning 4-domains subfamily A member 4A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC108273082</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MS4A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017337561.1">
        <property type="nucleotide sequence ID" value="XM_017482072.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S550"/>
    <dbReference type="GeneID" id="108273082"/>
    <dbReference type="KEGG" id="ipu:108273082"/>
    <dbReference type="OMA" id="ISCIMAC"/>
    <dbReference type="OrthoDB" id="10071849at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007237">
        <property type="entry name" value="CD20-like"/>
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        <property type="entry name" value="MS4A"/>
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    <dbReference type="PANTHER" id="PTHR23320:SF128">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MEMBER 4A"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF04103">
        <property type="entry name" value="CD20"/>
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            <begin position="52"/>
            <end position="74"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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    <feature type="transmembrane region" description="Helical" evidence="6">
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="167"/>
            <end position="188"/>
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    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004141"/>
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    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017337561.1"/>
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    <sequence length="250" mass="26495" checksum="F84893B9EEBE009D" modified="2017-12-20" version="1">MASAPIPLTNVGSGYTIVTQVLPSSTSSNTADQNSPQTLGPLQKFLKGEPKALGTVQIMIGSLMILFGIVMAVYPQTISVYSGVMFWGSLLHIAAGALAVSASNKLNACVVKGAMVVNIFSTIAAAIAIIMLSLDFMFGAFYRPCYYYDFSFVCHINYITESRTDGIVGVLLVFSLLQFAISIAISAFTCKATCTNEPTLNIVNVVPNPEGCVPVVSSFPAHHAQPGDSTMNAFAMSSAPPAYREKSQPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S5C6</accession>
    <name>A0A2D0S5C6_ICTPU</name>
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        <submittedName>
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        </submittedName>
    </protein>
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        <name type="scientific">Ictalurus punctatus</name>
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        <name type="synonym">Silurus punctatus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
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            <taxon>Siluriformes</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="23">SDA103</strain>
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    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
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    <comment type="similarity">
        <text evidence="1">Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.</text>
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        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    </dbReference>
    <dbReference type="CDD" id="cd06366">
        <property type="entry name" value="PBP1_GABAb_receptor"/>
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    </dbReference>
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        <property type="entry name" value="ANF_lig-bd_rcpt"/>
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        <property type="entry name" value="GPCR3_GABA-B"/>
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        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
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        <property type="entry name" value="GPCR_3_GABA_rcpt_B1"/>
    </dbReference>
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        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
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        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519">
        <property type="entry name" value="GABA-B RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10519:SF77">
        <property type="entry name" value="GAMMA-AMINOBUTYRIC ACID TYPE B RECEPTOR SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01177">
        <property type="entry name" value="GABAB1RECPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01176">
        <property type="entry name" value="GABABRECEPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="12 24" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="14" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039909579" evidence="20">
        <location>
            <begin position="20"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="588"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="625"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="664"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="706"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="766"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="807"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="832"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="22">
        <location>
            <begin position="95"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="21">
        <location>
            <begin position="595"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="864"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="916"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="864"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="960"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
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    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
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    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_017337959.2"/>
        </source>
    </evidence>
    <sequence length="996" mass="112802" checksum="E4B870B3332AF3D9" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S663</accession>
    <name>A0A2D0S663_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">NAD(P)(+)--arginine ADP-ribosyltransferase</fullName>
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        <alternativeName>
            <fullName evidence="14">Mono(ADP-ribosyl)transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC108273493</name>
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    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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            <taxon>Actinopterygii</taxon>
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        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>L-arginyl-[protein] + NAD(+) = N(omega)-(ADP-D-ribosyl)-L-arginyl-[protein] + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19149"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:15087"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:142554"/>
            <dbReference type="EC" id="2.4.2.31"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the Arg-specific ADP-ribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.31" evidence="14"/>
    <dbReference type="RefSeq" id="XP_017338248.1">
        <property type="nucleotide sequence ID" value="XM_017482759.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S663"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005637"/>
    <dbReference type="GeneID" id="108273493"/>
    <dbReference type="KEGG" id="ipu:108273493"/>
    <dbReference type="OrthoDB" id="423533at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106274">
        <property type="term" value="F:NAD+-protein-arginine ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.176.10:FF:000001">
        <property type="entry name" value="NAD(P)(+)--arginine ADP-ribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.176.10">
        <property type="entry name" value="Toxin ADP-ribosyltransferase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050999">
        <property type="entry name" value="ADP-ribosyltransferase_ARG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000768">
        <property type="entry name" value="ART"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10339">
        <property type="entry name" value="ADP-RIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10339:SF25">
        <property type="entry name" value="SECRETED EXOENZYME S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01129">
        <property type="entry name" value="ART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00970">
        <property type="entry name" value="RIBTRNSFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51996">
        <property type="entry name" value="TR_MART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 14" id="KW-0520">NAD</keyword>
    <keyword evidence="9 14" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0800">Toxin</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0843">Virulence</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017338248.1"/>
        </source>
    </evidence>
    <sequence length="258" mass="29954" checksum="262DD7989E11E23E" modified="2017-12-20" version="1">MNKAVTFSTFAKNAATAVIIMSLVCHTLSLNWTSDPVFQLDMAMDSVDDQYIDCENDMYNLTITTILPNELNSNKEFRTFWNMFNIPDYYERSIKVYTDTELYAELNNKVRSGRKSYKHNFNYKAFHFLLTRGIQIRKVNKCTDVFRRTGNHYVTKVLREMRFGQFASSSLTKNPNGFGNASCFKIKTCFGANISNISVFKHEKEVLIPPYEKFKITNIEKNSNEMNCEVLYTLESSGRCSEMNCKLLNRKKKMCCIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S8Q0</accession>
    <name>A0A2D0S8Q0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Cilia- and flagella-associated protein 57 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">LOC108273918</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7 8">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017339102.1">
        <property type="nucleotide sequence ID" value="XM_017483613.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017339112.1">
        <property type="nucleotide sequence ID" value="XM_017483623.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S8Q0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006246"/>
    <dbReference type="GeneID" id="108273918"/>
    <dbReference type="KEGG" id="ipu:108273918"/>
    <dbReference type="OrthoDB" id="10251741at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052993">
        <property type="entry name" value="CFA-57"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32215">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32215:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101908">
        <property type="entry name" value="Putative isomerase YbhE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7 8" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="341"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="5">
        <location>
            <begin position="349"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="463"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="581"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="660"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="996"/>
            <end position="1047"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017339102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017339112.1"/>
        </source>
    </evidence>
    <sequence length="1157" mass="134336" checksum="7F86BC0380F52951" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SBP1</accession>
    <name>A0A2D0SBP1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">wnk4a</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017339650.1">
        <property type="nucleotide sequence ID" value="XM_017484161.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000021147"/>
    <dbReference type="GeneID" id="108274180"/>
    <dbReference type="KEGG" id="ipu:108274180"/>
    <dbReference type="CTD" id="100330953"/>
    <dbReference type="OMA" id="HPNMWPS"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14033">
        <property type="entry name" value="STKc_WNK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000007">
        <property type="entry name" value="Serine/threonine-protein kinase WNK1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000006">
        <property type="entry name" value="Serine/threonine-protein kinase WNK1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000494">
        <property type="entry name" value="serine/threonine-protein kinase WNK2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056865">
        <property type="entry name" value="CCTL2_WNK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024678">
        <property type="entry name" value="Kinase_OSR1/WNK_CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050588">
        <property type="entry name" value="WNK_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13902">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24889">
        <property type="entry name" value="CCTL2_WNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12202">
        <property type="entry name" value="OSR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="141"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="58"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="487"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="651"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="810"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="893"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1050"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1287"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1442"/>
            <end position="1461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1478"/>
            <end position="1514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="66"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="495"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="656"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="810"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="910"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1061"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1077"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1102"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1157"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1290"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1306"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1480"/>
            <end position="1496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1497"/>
            <end position="1514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017339650.1"/>
        </source>
    </evidence>
    <sequence length="1514" mass="167502" checksum="074296530DE53DE1" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SGK6</accession>
    <name>A0A2D0SGK6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Plexin-B1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">plxnb1a</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Receptor for SEMA4D. Plays a role in GABAergic synapse development. Mediates SEMA4A- and SEMA4D-dependent inhibitory synapse development. Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017486363.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000009715"/>
    <dbReference type="GeneID" id="108275501"/>
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    <dbReference type="OMA" id="TPYFYET"/>
    <dbReference type="OrthoDB" id="125363at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002116">
        <property type="term" value="C:semaphorin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017154">
        <property type="term" value="F:semaphorin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048675">
        <property type="term" value="P:axon extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007162">
        <property type="term" value="P:negative regulation of cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050772">
        <property type="term" value="P:positive regulation of axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01180">
        <property type="entry name" value="IPT_plexin_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12793">
        <property type="entry name" value="RasGAP_plexin_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000131">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000010">
        <property type="entry name" value="Plexin B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000012">
        <property type="entry name" value="Plexin B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000126">
        <property type="entry name" value="Plexin B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000705">
        <property type="entry name" value="Plexin B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000120">
        <property type="entry name" value="Plexin b1a"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Plexin B2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_E-set"/>
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    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
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        <property type="entry name" value="Plexin"/>
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        <property type="entry name" value="Plexin_RBD"/>
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        <property type="entry name" value="Plexin_repeat"/>
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        <property type="entry name" value="PSI"/>
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        <property type="entry name" value="PSI_Plexin-B"/>
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    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
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    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
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    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
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        <property type="entry name" value="TIG1_plexin"/>
    </dbReference>
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        <property type="entry name" value="TIG2_plexin"/>
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    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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    <dbReference type="PANTHER" id="PTHR22625">
        <property type="entry name" value="PLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PLEXIN-B1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08337">
        <property type="entry name" value="Plexin_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sema"/>
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        <property type="entry name" value="TIG"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="TIG_2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="TIG_plexin"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
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        <property type="entry name" value="Plexin repeat"/>
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        <property type="entry name" value="Sema domain"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1515"/>
            <end position="1535"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="19">
        <location>
            <begin position="36"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1295"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="1303"/>
            <end position="1312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00057668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017341852.1"/>
        </source>
    </evidence>
    <sequence length="2158" mass="240049" checksum="8FB358A2E76E803C" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SJ12</accession>
    <name>A0A2D0SJ12_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Cation-transporting ATPase 13A2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">atp13a2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017342481.1">
        <property type="nucleotide sequence ID" value="XM_017486992.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SJ12"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032296"/>
    <dbReference type="GeneID" id="108275900"/>
    <dbReference type="KEGG" id="ipu:108275900"/>
    <dbReference type="CTD" id="23400"/>
    <dbReference type="OrthoDB" id="48943at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015662">
        <property type="term" value="F:P-type ion transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015203">
        <property type="term" value="F:polyamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061909">
        <property type="term" value="P:autophagosome-lysosome fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016243">
        <property type="term" value="P:regulation of autophagosome size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010821">
        <property type="term" value="P:regulation of mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07542">
        <property type="entry name" value="P-type_ATPase_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000023">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000060">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000045">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047819">
        <property type="entry name" value="P5A-ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047821">
        <property type="entry name" value="P5B-type_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF2">
        <property type="entry name" value="POLYAMINE-TRANSPORTING ATPASE 13A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12409">
        <property type="entry name" value="P5-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01229">
        <property type="entry name" value="COF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="407"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="443"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="914"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="945"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="975"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1029"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1059"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1098"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="domain" description="P5B-type ATPase N-terminal" evidence="14">
        <location>
            <begin position="36"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="13">
        <location>
            <begin position="171"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="12">
        <location>
            <begin position="271"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF12409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017342481.1"/>
        </source>
    </evidence>
    <sequence length="1172" mass="129234" checksum="9E3F946C54716EAF" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SKD4</accession>
    <name>A0A2D0SKD4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Synaptonemal complex protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">sycp2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SYCP2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017342951.1">
        <property type="nucleotide sequence ID" value="XM_017487462.3"/>
    </dbReference>
    <dbReference type="GeneID" id="108276099"/>
    <dbReference type="KEGG" id="ipu:108276099"/>
    <dbReference type="CTD" id="10388"/>
    <dbReference type="OrthoDB" id="10256849at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000779">
        <property type="term" value="C:condensed chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000800">
        <property type="term" value="C:lateral element"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007143">
        <property type="term" value="P:female meiotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007140">
        <property type="term" value="P:male meiotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024835">
        <property type="entry name" value="SYCP2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041322">
        <property type="entry name" value="SYCP2_ARLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040560">
        <property type="entry name" value="SYCP2_SLD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15607:SF12">
        <property type="entry name" value="SYNAPTONEMAL COMPLEX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15607">
        <property type="entry name" value="SYNAPTONEMAL COMPLEX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18581">
        <property type="entry name" value="SYCP2_ARLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18584">
        <property type="entry name" value="SYCP2_SLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Synaptonemal complex protein 2 armadillo-repeat-like" evidence="7">
        <location>
            <begin position="9"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Synaptonemal complex protein 2 Spt16M-like" evidence="8">
        <location>
            <begin position="279"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="629"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="678"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="869"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1115"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1337"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="638"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="705"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="928"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1125"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1349"/>
            <end position="1362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017342951.1"/>
        </source>
    </evidence>
    <sequence length="1633" mass="182686" checksum="87EF24937138B40F" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SKP9</accession>
    <name>A0A2D0SKP9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">WNT1-inducible-signaling pathway protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ccn5</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CCN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017343282.1">
        <property type="nucleotide sequence ID" value="XM_017487793.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SKP9"/>
    <dbReference type="GeneID" id="108276268"/>
    <dbReference type="KEGG" id="ipu:108276268"/>
    <dbReference type="CTD" id="8839"/>
    <dbReference type="OMA" id="RLVHHGC"/>
    <dbReference type="OrthoDB" id="365605at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045597">
        <property type="term" value="P:positive regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050941">
        <property type="entry name" value="CCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006207">
        <property type="entry name" value="Cys_knot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006208">
        <property type="entry name" value="Glyco_hormone_CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000867">
        <property type="entry name" value="IGFBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012395">
        <property type="entry name" value="IGFBP_CNN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017891">
        <property type="entry name" value="Insulin_GF-bd_Cys-rich_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043973">
        <property type="entry name" value="TSP1_CCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11348:SF22">
        <property type="entry name" value="CCN FAMILY MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11348">
        <property type="entry name" value="CONNECTIVE TISSUE GROWTH FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00007">
        <property type="entry name" value="Cys_knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00219">
        <property type="entry name" value="IGFBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19035">
        <property type="entry name" value="TSP1_CCN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00093">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036495">
        <property type="entry name" value="IGFBP_rP_CNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00041">
        <property type="entry name" value="CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00121">
        <property type="entry name" value="IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01185">
        <property type="entry name" value="CTCK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01225">
        <property type="entry name" value="CTCK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00222">
        <property type="entry name" value="IGFBP_N_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51323">
        <property type="entry name" value="IGFBP_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012090413" evidence="7">
        <location>
            <begin position="27"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="IGFBP N-terminal" evidence="10">
        <location>
            <begin position="21"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="9">
        <location>
            <begin position="100"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="CTCK" evidence="8">
        <location>
            <begin position="257"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017343282.1"/>
        </source>
    </evidence>
    <sequence length="345" mass="37916" checksum="22A56639889BAE45" modified="2017-12-20" version="1">MDKKVQNTYTLLARALLLYLCSEVCCQQCVRPCQCPSSVLACPEGVALVVDGCGCCQVCARQKGEACTNILVCDKQHGLECDYSASFPGDPGECVSQEELSCELNGVSYQEGEVFQPSCSTQCKCVGGGVTCVPLCGEDIRLPSSDCPYPQKVQLPGKCCKEWVCENSDNSVIQDAQTAYTDAQVMPALPGFQASPSLNCIYQSTEWSACSSTCGPGISTRVSNQNAACRLEQQIRLCKIRPCQTLPYQTPVWPRKCQPSYRSNVPTRLLHQGCYSTQFYRLRYCGLCTDGRCCTPYRTRTVTVTFRCPGGRLVRQAVMMIKSCVCHYNCPYSSGGTYRRLAPWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SP81</accession>
    <name>A0A2D0SP81_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Tsukushi</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC108276918</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017344518.1">
        <property type="nucleotide sequence ID" value="XM_017489029.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SP81"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000031030"/>
    <dbReference type="GeneID" id="108276918"/>
    <dbReference type="KEGG" id="ipu:108276918"/>
    <dbReference type="OMA" id="CIDTQEP"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050328">
        <property type="entry name" value="Dev_Immune_Receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373">
        <property type="entry name" value="SLIT RELATED LEUCINE-RICH REPEAT NEURONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24373:SF352">
        <property type="entry name" value="TSUKUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013333971" evidence="4">
        <location>
            <begin position="23"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017344518.1"/>
        </source>
    </evidence>
    <sequence length="346" mass="38181" checksum="A6AAA31777A2D5EC" modified="2017-12-20" version="1">MAILLEGRVSALLVLFFSTVKTCHPGCRCEEQSFGLFNSFSLMKVDCSGLGPNTIPVSIPLETSSLDLSSNGIRSIEGAVFSGPGYTTLSSLDLSNNLISSINGSVFSKLHYLETLDLSWNSILNLVEGFPGLPLTEVDLSDNRIHEINLNVFRVQDRGRPLNVDLSYNLIMSVIRNKLSQPLNIHSLNLAGNELSSVPSLRDISLRFLTLDDNPISVIDEQSFAGLKYLVHLSLSSMPELHTVKPQSLHDLQNLQVLDLSDNHKLKTLSAEVFRGLKSLQELNLSNCWLTSLPANILQLLPSIKSIALRDGVNCRRIQRQEQFHRHVGRNKAEAALTCDVTGVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T330</accession>
    <name>A0A2D0T330_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Anoctamin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13 14" type="primary">ano2b</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13 14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the anoctamin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017349216.1">
        <property type="nucleotide sequence ID" value="XM_017493727.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017349217.1">
        <property type="nucleotide sequence ID" value="XM_017493728.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T330"/>
    <dbReference type="GeneID" id="108279462"/>
    <dbReference type="KEGG" id="ipu:108279462"/>
    <dbReference type="CTD" id="566373"/>
    <dbReference type="OrthoDB" id="296386at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005229">
        <property type="term" value="F:intracellularly calcium-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032394">
        <property type="entry name" value="Anoct_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF20">
        <property type="entry name" value="ANOCTAMIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16178">
        <property type="entry name" value="Anoct_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="360"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="548"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="592"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="638"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="765"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="918"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin dimerisation" evidence="11">
        <location>
            <begin position="81"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="10">
        <location>
            <begin position="349"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="478"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="988"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="491"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="503"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1028"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1038"/>
            <end position="1047"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU280814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017349216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017349217.1"/>
        </source>
    </evidence>
    <sequence length="1047" mass="119997" checksum="A43B44D2CD936890" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0TA63</accession>
    <name>A0A2D0TA63_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DnaJ homolog subfamily B member 6a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">dnajb6a</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017351763.1">
        <property type="nucleotide sequence ID" value="XM_017496274.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0TA63"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000002323"/>
    <dbReference type="GeneID" id="108280836"/>
    <dbReference type="KEGG" id="ipu:108280836"/>
    <dbReference type="CTD" id="436626"/>
    <dbReference type="OMA" id="FTYDEYD"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000021">
        <property type="entry name" value="DnaJ (Hsp40) homolog, subfamily B, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043183">
        <property type="entry name" value="DNJB2/6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45168">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45168:SF4">
        <property type="entry name" value="SIMILAR TO DNAJ HOMOLOG SUBFAMILY B MEMBER 6 (HEAT SHOCK PROTEIN J2) (HSJ-2) (MRJ) (MDJ4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="284"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="218"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="297"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="332"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017351763.1"/>
        </source>
    </evidence>
    <sequence length="342" mass="36689" checksum="1A08DC1EB30584E1" modified="2017-12-20" version="1">MVEYYQILGVQKDASPDDIKKAYRKLALKWHPDKNPDNKDEAERKFKEISEAYEVLSDASKRSIYDQYGKEGLSGGGGGGRYHNGDPFEGFTFRSPDEVFRDFFGGRDPFADFFGDGLFGDDFFGGGRRPQHRGMSRSRTGGSFFSSGFGGFGGFGSVPSFGPAFSSFDGGFSSFGHMGPMGQLGPMGQMGQMGQMGQMGQLGQMGGGGFTSFSSSSFGGGGGGGGGGSGMGNFRSVSTSTKFVNGRKITTKRIVENGQERVEVEEDGQLKSLTINGQALDLGLGAERKQNKSLPVSSSNSAHSNSSHSQSSQRERQPTTEQNGMKRKRPPLKSETKKSRES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979DZE8</accession>
    <name>A0A979DZE8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">ksr2</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_047005688.1">
        <property type="nucleotide sequence ID" value="XM_047149732.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979DZE8"/>
    <dbReference type="GeneID" id="108255889"/>
    <dbReference type="KEGG" id="ipu:108255889"/>
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    <dbReference type="OrthoDB" id="774951at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="FunFam" id="1.10.510.10:FF:000107">
        <property type="entry name" value="kinase suppressor of Ras 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000034">
        <property type="entry name" value="Kinase suppressor of Ras 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000031">
        <property type="entry name" value="Kinase suppressor of Ras 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000010">
        <property type="entry name" value="Putative kinase suppressor of Ras 1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR025561">
        <property type="entry name" value="KSR_SAM-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
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    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046861">
        <property type="entry name" value="SAM_KSR1_N"/>
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    <dbReference type="InterPro" id="IPR046933">
        <property type="entry name" value="SAM_KSR1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050167">
        <property type="entry name" value="Ser_Thr_protein_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257:SF775">
        <property type="entry name" value="KINASE SUPPRESSOR OF RAS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257">
        <property type="entry name" value="SERINE-THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13543">
        <property type="entry name" value="SAM_KSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20406">
        <property type="entry name" value="SAM_KSR1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 22" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="20">
        <location>
            <begin position="424"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="683"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="159"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="265"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="518"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="173"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="534"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="550"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_047005688.1"/>
        </source>
    </evidence>
    <sequence length="970" mass="109440" checksum="5EC81FD6AFF3264E" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EG62</accession>
    <name>A0A979EG62_ICTPU</name>
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        <submittedName>
            <fullName evidence="6">Hormone-sensitive lipase isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">lipeb</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047006225.1">
        <property type="nucleotide sequence ID" value="XM_047150269.2"/>
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    <dbReference type="CTD" id="557893"/>
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        <property type="component" value="Chromosome 23"/>
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    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004771">
        <property type="term" value="F:sterol ester esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010468">
        <property type="entry name" value="HSL_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033140">
        <property type="entry name" value="Lipase_GDXG_put_SER_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23025:SF1">
        <property type="entry name" value="HORMONE-SENSITIVE LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23025">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06350">
        <property type="entry name" value="HSL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01174">
        <property type="entry name" value="LIPASE_GDXG_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hormone-sensitive lipase N-terminal" evidence="3">
        <location>
            <begin position="43"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="4">
        <location>
            <begin position="392"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="4">
        <location>
            <begin position="722"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_047006225.1"/>
        </source>
    </evidence>
    <sequence length="860" mass="94640" checksum="09A71B872B5308D9" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EIA7</accession>
    <name>A0A979EIA7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC108281035</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108281035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047005251.2">
        <property type="nucleotide sequence ID" value="XM_047149295.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EIA7"/>
    <dbReference type="GeneID" id="108281035"/>
    <dbReference type="KEGG" id="ipu:108281035"/>
    <dbReference type="OrthoDB" id="8936013at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_047005251.2"/>
        </source>
    </evidence>
    <sequence length="484" mass="54257" checksum="BB6278E5405DCEFE" modified="2023-06-28" version="2">MTLPGNHGDGGRYEYLPNTPRDLEDTRTVPRHDPEFSPKVAQKQNCLLSCCSSNMEEPSSFQSQTEDEDYMLEKTVGYLPLHHELDSGLGWTDGSFHQGELSGVETEEGLDECHAHQGGRGARVRGGSPSSESFISSELSDSGFYSVSTAEFLRFQRLLEKRMRVYNSRLHHQGELQSLCMDAQKAHRELEAIPEALAMQPDSEGVEMDAQPPQMVRVASVQLRKSERPCLNRHSTSSAAIFSTCSSPSGQQRGATPHASAGMLRRSRTLHHRPVHVEQRRRASHPASPNHCSVTLNRYAHALQQQLTEETETDVLLYIMLTTSYILRNMLISLHTPFMPLFPPNTHTTPTVHSPHMLHTMTMPTLRSPPNTNPTPMIHMHYTPHTMAMPTLRSPSNTHITPMVHLHHTPHTTVTLRSPSNTHTMPTVHLHHMVHTTATPTLHSPPKTHTMPMVYSQHTPCPTTMLHSPPNIHITWHGCAPNML</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EK44</accession>
    <name>A0A979EK44_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15 16">Class E basic helix-loop-helix protein 41</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15 16" type="primary">bhlhe41</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15 16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_047008815.1">
        <property type="nucleotide sequence ID" value="XM_047152859.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053533923.1">
        <property type="nucleotide sequence ID" value="XM_053677948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EK44"/>
    <dbReference type="GeneID" id="124626808"/>
    <dbReference type="KEGG" id="ipu:124626808"/>
    <dbReference type="CTD" id="79365"/>
    <dbReference type="GeneTree" id="ENSGT00940000161014"/>
    <dbReference type="OrthoDB" id="690068at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19750">
        <property type="entry name" value="bHLH-O_DEC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000020">
        <property type="entry name" value="class E basic helix-loop-helix protein 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07527">
        <property type="entry name" value="Hairy_orange"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00511">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158457">
        <property type="entry name" value="Orange domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BHLH" evidence="12">
        <location>
            <begin position="44"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="13">
        <location>
            <begin position="131"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="357"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="366"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="384"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="397"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_047008815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_053533923.1"/>
        </source>
    </evidence>
    <sequence length="409" mass="44876" checksum="255525E8F0BDFDD8" modified="2023-02-22" version="1">MDERISRLQDRAFLDHADFLGVDYSSLYMCKTKRGMKREEGKDAYKLPHRLIEKKRRDRINECIGQLKDLLPEHLKLTTLGHLEKAVVLELTLKHLNALTAVTEQQHQKILALQNGDRTLKSSIRADLDAFHSGFQACAKEVLKYLGTVEKWTSCEQRCTQLLEHLHKVLARLVQPQERDGQANCVPVIQRTQNPEANENDTDTDSGYGGEAEKSDARAEKACDTVRVAGVKIKQEFGDERVAKKPKMSWSEKSGAESAEQPNLAFMNSLMGMAGVGQQTPFCVPFYFINPSAAGSYMPFFDKSSMEKLVYPALSGPFPWLYPGITAHTSAAAAAAAAAAAAAAFPAGSLEKSLGFGAASKENDCPSPSNDAKQSNRLGASSPTDEEQNSDPDLNEDQTQMKETSSTTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979ENP1</accession>
    <name>A0A979ENP1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Coiled-coil domain-containing protein 191 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ccdc191</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_047150130.2"/>
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        <property type="component" value="Chromosome 23"/>
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            <begin position="362"/>
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        <location>
            <begin position="582"/>
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        <location>
            <begin position="625"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_047006086.1"/>
        </source>
    </evidence>
    <sequence length="893" mass="106515" checksum="806C87F1ABDFE5B7" modified="2023-02-22" version="1"> </sequence>
</entry>
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        <name type="scientific">Ictalurus punctatus</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047014706.2">
        <property type="nucleotide sequence ID" value="XM_047158750.2"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="8062037at2759"/>
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        <property type="component" value="Chromosome 11"/>
    </dbReference>
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        <property type="entry name" value="LNP1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_047014706.2"/>
        </source>
    </evidence>
    <sequence length="279" mass="32439" checksum="98F551EDA4C01968" modified="2023-06-28" version="2">RLETHVSYRPDLNELGDQIICTSSEPRSSFPFPLRSSRYLRVFPSGAPARAPHTPASKLSLRLFPSIIVDNDDDDDGNFTKWMSSYWGHGGRDERAKERKRSFRRSRQSTSDRRASLPCMSQLEAMRLKHPSTPSHLKSHEEKEVRSHPHARRVSSDEYRGNKAVGAESRISTVPELTESFKRMLRFRNHRVPSLTDSDSACLICREEMRRRGGEPGRSGQELHCSHRLHKEVRRLEQGARRRSQSSAAVRENRKEVPFCSEQEEHRLPRQHRSLRRQR</sequence>
</entry>
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        <submittedName>
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        <name evidence="3" type="primary">LOC108281062</name>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017305499.2">
        <property type="nucleotide sequence ID" value="XM_017450010.3"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_053674135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QUE8"/>
    <dbReference type="GeneID" id="108281062"/>
    <dbReference type="KEGG" id="ipu:108254737"/>
    <dbReference type="KEGG" id="ipu:108281062"/>
    <dbReference type="OMA" id="MSYLAPM"/>
    <dbReference type="OrthoDB" id="3758675at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="CDD" id="cd09302">
        <property type="entry name" value="Jacalin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PFM_Dln1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.100.10.30">
        <property type="entry name" value="Jacalin-like lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Proaerolysin, chain A, domain 3"/>
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    </dbReference>
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    <dbReference type="InterPro" id="IPR036404">
        <property type="entry name" value="Jacalin-like_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34007">
        <property type="entry name" value="AEROLYSIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34007:SF1">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01419">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF56973">
        <property type="entry name" value="Aerolisin/ETX pore-forming domain"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51752">
        <property type="entry name" value="JACALIN_LECTIN"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="4"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053530110.1"/>
        </source>
    </evidence>
    <sequence length="312" mass="34077" checksum="FCF748D655A43323" modified="2023-06-28" version="1">MSYLSPILVIGGGGGKSFNFNGVGNGATLKKIWVWAGGWQIKAIKVWLTDGQIGQFGEPAGRFSEFSFEDGEHFTSLSLWGNGAGTRLGAIKFRTNRSREFFAQMTDWGLKTEYPIDVGSGICVGITGHSGADMDSLGFEFINTVKSTKLTNVQYPTLHDVIPNVAVEEIKSTTYENKSTQTQEYKLETSKTIIRKSSWSVTNKLEFTFSMEVKAGIPEVVEVSAGFGFTVGTESSYGLENSEERTELFSFPVKVPPGKTVHVDITIGRATVDLPYTGTVQITCYNGSVLEFPTSGIYKGLNYTDAKVVVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QUU1</accession>
    <name>A0A9F7QUU1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Urea transporter 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">slc14a2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>urea(in) = urea(out)</text>
            <dbReference type="Rhea" id="RHEA:32799"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the urea transporter family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053530604.1">
        <property type="nucleotide sequence ID" value="XM_053674629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QUU1"/>
    <dbReference type="GeneID" id="108255523"/>
    <dbReference type="CTD" id="8170"/>
    <dbReference type="OrthoDB" id="426293at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015204">
        <property type="term" value="F:urea transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000002">
        <property type="entry name" value="urea transporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004937">
        <property type="entry name" value="Urea_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10464">
        <property type="entry name" value="UREA TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10464:SF4">
        <property type="entry name" value="UREA TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03253">
        <property type="entry name" value="UT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="293"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="357"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="382"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="427"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053530604.1"/>
        </source>
    </evidence>
    <sequence length="503" mass="56213" checksum="8888FDECB7731239" modified="2023-06-28" version="1">MWASWWAHDLSTLPGTPSGPAAFLGFTARNIRLTSCSLTELQPLMANTVLQTQKNSQLQQNEQQHTQTESSKLQKIKVKLTRFASYFSGNMAPFDTWMKEQCILLQICDWVLRGAAQVMFVNNPLSGLIIFAGLILQNRWWALNGFVGTLFATISALILKQNRGAIAAGLYGYNGILVGLLMAVFSNAGDWYWWLLLPNIFMSMACPIISSALASINSRWDLPVLNLPFNILVCLHMVATGHYNHHFPQVLIQPITSFPNITWSELEYTLLFQSIPVGIGQVYGCDNPWTGGIFIVALFISSPITCMHAVIGSTIGMVSGLALAAPFQSIYFGLWGYNCALACIAIGGIYYALTWQVHLLSIVCAFFCAYLGFAISNVMATFGLPACTWPFCLSVLTFLLITTETKTIYKLPLSIVTYPEKNLIFYCKVKEEKAEKKQKKVQEEMLQEKDKEAQMTKDMDVKIMMENQEKEQHNVCIKISRGHLENLSITGNTEPTTLELTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R1I8</accession>
    <name>A0A9F7R1I8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cartilage intermediate layer protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC128632930</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053537080.1">
        <property type="nucleotide sequence ID" value="XM_053681105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R1I8"/>
    <dbReference type="GeneID" id="128632930"/>
    <dbReference type="KEGG" id="ipu:128632930"/>
    <dbReference type="OrthoDB" id="6049857at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039675">
        <property type="entry name" value="CILP1/CILP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025155">
        <property type="entry name" value="WxxW_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15031:SF4">
        <property type="entry name" value="CARTILAGE INTERMEDIATE LAYER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15031">
        <property type="entry name" value="CARTILAGE INTERMEDIATE LAYER PROTEIN CLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13330">
        <property type="entry name" value="Mucin2_WxxW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039942430" evidence="5">
        <location>
            <begin position="18"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="WxxW" evidence="6">
        <location>
            <begin position="29"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053537080.1"/>
        </source>
    </evidence>
    <sequence length="124" mass="13872" checksum="B4752AE3541DEE80" modified="2023-06-28" version="1">MKLLIFGVILIATHTGAQDLEKRPAVCYTQWFDRDNPTGNGDYETLKDLRAEYPKKICSKPLTIQAATLTGVPAESIGQVFQVYDTVLGFACVNSQQKNDECCLDYKVRFGCPCPWPPIKPEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R1Y8</accession>
    <name>A0A9F7R1Y8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5 6">TBC1 domain family member 10A isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5 6 7 8" type="primary">LOC128630389</name>
    </gene>
    <organism evidence="3 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4 5 6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053534876.1">
        <property type="nucleotide sequence ID" value="XM_053678901.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534877.1">
        <property type="nucleotide sequence ID" value="XM_053678902.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534878.1">
        <property type="nucleotide sequence ID" value="XM_053678903.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534879.1">
        <property type="nucleotide sequence ID" value="XM_053678904.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534880.1">
        <property type="nucleotide sequence ID" value="XM_053678905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R1Y8"/>
    <dbReference type="GeneID" id="128630389"/>
    <dbReference type="KEGG" id="ipu:128630389"/>
    <dbReference type="OrthoDB" id="159449at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000008">
        <property type="entry name" value="TBC1 domain family member 10A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF4">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 10A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053534876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053534877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_053534878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053534879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053534880.1"/>
        </source>
    </evidence>
    <sequence length="252" mass="29076" checksum="D835F6B96B6BDB49" modified="2023-06-28" version="1">MMEWFMCAFSRTLPWASVLRVWDMFLCDGVKMIFCVGLVVLTSMLGTRDKLKACPGHYETMEVLRAIEPRYMQEGFFVLQVLELQVSAQDVEREQRTQLKRWKKKRGEPGPKLPQRMHSARTIMATEPHTHQDLRQKPTIMVQYPSVPEEKSEPNLRKKRGSLKKNQALIPNPYALPGESKPSQILDIQLLNQENLNPVLPNPPSHPPRLPTMQENQVKETSTSTERLVQRPRIPPSIRNRGNLSLCNIYPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R2C5</accession>
    <name>A0A9F7R2C5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">CD48 antigen-like isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC108261143</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053537515.1">
        <property type="nucleotide sequence ID" value="XM_053681540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R2C5"/>
    <dbReference type="GeneID" id="108261143"/>
    <dbReference type="OrthoDB" id="9835793at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CD2/SLAM_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12080:SF48">
        <property type="entry name" value="IMMUNOGLOBULIN SUBTYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12080">
        <property type="entry name" value="SIGNALING LYMPHOCYTIC ACTIVATION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_053537515.1"/>
        </source>
    </evidence>
    <sequence length="216" mass="23376" checksum="443A80DFD8F34759" modified="2023-06-28" version="1">MRYCHEYRVSMSGALMFWILQGVCGVLADAVMVLKVGSSLDLPVQYPIESLLLVQWVFNGKIFAEYSTDQSYTLLESQFSGRLKEDNDRVGVTVQDLQPQDSGTFSVTAEGTETQLPTQTFKVYIQIPITAVQIGKNQAWILSTNSCDVDVKCAAPGAESVSYLWSGYKTGSGAQLQFSLSPAEGAVTLNCTAANNVSSSSATETLNCSVKITLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R609</accession>
    <name>A0A9F7R609_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ras GTPase-activating protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rasa2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053534037.1">
        <property type="nucleotide sequence ID" value="XM_053678062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R609"/>
    <dbReference type="GeneID" id="108264552"/>
    <dbReference type="KEGG" id="ipu:108264552"/>
    <dbReference type="CTD" id="5922"/>
    <dbReference type="OrthoDB" id="1562946at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04010">
        <property type="entry name" value="C2B_RasA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000011">
        <property type="entry name" value="Ras GTPase-activating protein 2 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023152">
        <property type="entry name" value="RasGAP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF21">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="153"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GAP" evidence="4">
        <location>
            <begin position="358"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="607"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_053534037.1"/>
        </source>
    </evidence>
    <sequence length="827" mass="94050" checksum="E39B43FB13AF1F5B" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R937</accession>
    <name>A0A9F7R937_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Dedicator of cytokinesis protein 8 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">dock8</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the DOCK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053536280.1">
        <property type="nucleotide sequence ID" value="XM_053680305.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108265417"/>
    <dbReference type="CTD" id="81704"/>
    <dbReference type="OrthoDB" id="47328at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031252">
        <property type="term" value="C:cell leading edge"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903905">
        <property type="term" value="P:positive regulation of establishment of T cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
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                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
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                <person name="Duke M.V."/>
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                <person name="Kucuktas H."/>
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</entry>
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
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                <person name="Yuan Z."/>
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                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
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                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">SDA103</strain>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="2">Blood</tissue>
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    </dbReference>
    <dbReference type="Pfam" id="PF14469">
        <property type="entry name" value="AKAP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_053539665.1"/>
        </source>
    </evidence>
    <sequence length="174" mass="20193" checksum="6157C829D321E70B" modified="2023-06-28" version="1">MDTKPGDDLLDLRASELVKSVLDGAQCTLTRAPTPDSNQLDFKIRNIDWVACKDFTISVGKTQIEQYMSTWEVEPRWLFSVQFLQENELEFEKQFHYRALWSMPSRRCPVPHSTASVYFTFSTSSTKPSTMPVRVRFQVESSETVHTPGKTRFREKWLKDVIESKALLMDTITF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RBF9</accession>
    <name>A0A9F7RBF9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein LOC108260598</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC108260598</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053533443.1">
        <property type="nucleotide sequence ID" value="XM_053677468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RBF9"/>
    <dbReference type="GeneID" id="108260598"/>
    <dbReference type="KEGG" id="ipu:108260598"/>
    <dbReference type="OrthoDB" id="8965174at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.960">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000082">
        <property type="entry name" value="SEA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036364">
        <property type="entry name" value="SEA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01390">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82671">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50024">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="SEA" evidence="2">
        <location>
            <begin position="98"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="60"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053533443.1"/>
        </source>
    </evidence>
    <sequence length="244" mass="25851" checksum="D502B41586C1DE24" modified="2023-06-28" version="1">MVINSFRNGSIVTNSTLEFTANGTIPTNSTVQDALVNATNTTSSLNIIPTSINVTQDPVTNNSSTNTSTNSTSTNTSTNSTTNTSSTNTTSTNTTNTPAVAAAFNLVFSINETFYPALANISSPNFTAKAQSIRNQIEPVYRKIFNNFIRMVINSFRNGSILTNCSLEFSSNGTIPTVSLVKEALLNATSTTSSLNIIPSSINVTQTFGNSTSNSTSNSPVIASSLSMIWMSLLSLLLSVALHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RC92</accession>
    <name>A0A9F7RC92_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">E3 ubiquitin-protein ligase RNF130 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">rnf130</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053537975.1">
        <property type="nucleotide sequence ID" value="XM_053682000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RC92"/>
    <dbReference type="GeneID" id="108268926"/>
    <dbReference type="KEGG" id="ipu:108268926"/>
    <dbReference type="CTD" id="55819"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02122">
        <property type="entry name" value="PA_GRAIL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16803">
        <property type="entry name" value="RING-H2_RNF130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000003">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000009">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ATL42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539:SF28">
        <property type="entry name" value="RING FINGER PROTEIN 130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039897634" evidence="11">
        <location>
            <begin position="32"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="403"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="277"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="371"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_053537975.1"/>
        </source>
    </evidence>
    <sequence length="436" mass="48351" checksum="0721E27F8F41F4B6" modified="2023-06-28" version="1">MQKRRRTSSSCVTLFLWSVLLSLVLPRCVSGAGTDKTDKTVVNKEEYYSATINATVQDVRGNAKRVISKEDGRYGQNSPRSEVKGIIITPAAVNGVVDMQGCCPYTRFIIPPKTTHWIALLQRGKCMFKEKILKAAAFNASAVLIYNNSSKEDTVTMAHEGTGDTVAVMITESLGKDLLSFLEKNETVWVSVSVGSRGSVKNLNRGSLVFVSVSFIVLMIISSAWLIFYFIQKIRDTNARDRSQRRLGDAAKKAISKLTTRTVKRGDKEAEPDFNHCAVCIEGYQLNDVVRILPCKHVFHKMCVDPWLNEHCTCPMCKLNILKALGIMPNLKCVDNVAFDMERMTRTHSSSQRVALMDVSSETSVSVEPLTRSGSSQLLSESELSHTHTHRSGEINIAVTKEWFIVGSFAVLSVLTLCYMIIRATGNYDNTALDTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RE55</accession>
    <name>A0A9F7RE55_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Nuclear protein localization protein 4 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">nploc4</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Acts as a negative regulator of type I interferon production via the complex formed with VCP and UFD1, which binds to RIGI and recruits RNF125 to promote ubiquitination and degradation of RIGI.</text>
    </comment>
    <comment type="pathway">
        <text evidence="14">Protein degradation; proteasomal ubiquitin-dependent pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Heterodimer with UFD1. The heterodimer binds ubiquitinated proteins. The heterodimer binds to VCP and inhibits Golgi membrane fusion. Interacts with ZFAND2B; probably through VCP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NPL4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053541120.1">
        <property type="nucleotide sequence ID" value="XM_053685145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RE55"/>
    <dbReference type="GeneID" id="108274306"/>
    <dbReference type="KEGG" id="ipu:108274306"/>
    <dbReference type="CTD" id="55666"/>
    <dbReference type="OrthoDB" id="10251089at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08061">
        <property type="entry name" value="MPN_NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.380:FF:000008">
        <property type="entry name" value="nuclear protein localization protein 4 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000084">
        <property type="entry name" value="nuclear protein localization protein 4 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000012">
        <property type="entry name" value="nuclear protein localization protein 4 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016563">
        <property type="entry name" value="Npl4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007717">
        <property type="entry name" value="NPL4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024682">
        <property type="entry name" value="Npl4_Ub-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007716">
        <property type="entry name" value="NPL4_Zn-bd_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12710">
        <property type="entry name" value="NUCLEAR PROTEIN LOCALIZATION 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12710:SF0">
        <property type="entry name" value="NUCLEAR PROTEIN LOCALIZATION PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05021">
        <property type="entry name" value="NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11543">
        <property type="entry name" value="UN_NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05020">
        <property type="entry name" value="zf-NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010052">
        <property type="entry name" value="Polyub_prc_Npl4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MPN" evidence="20">
        <location>
            <begin position="235"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="19">
        <location>
            <begin position="588"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="85"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00061946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00074519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_053541120.1"/>
        </source>
    </evidence>
    <sequence length="616" mass="68665" checksum="753A0078A6093F04" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RHS1</accession>
    <name>A0A9F7RHS1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Roundabout homolog 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">robo2</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the immunoglobulin superfamily. ROBO family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053543145.1">
        <property type="nucleotide sequence ID" value="XM_053687170.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108277721"/>
    <dbReference type="KEGG" id="ipu:108277721"/>
    <dbReference type="CTD" id="6092"/>
    <dbReference type="OrthoDB" id="428111at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022603">
        <property type="term" value="P:regulation of anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd07693">
        <property type="entry name" value="IgC_1_Robo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05726">
        <property type="entry name" value="IgI_4_Robo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20952">
        <property type="entry name" value="IgI_5_Robo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000053">
        <property type="entry name" value="Roundabout guidance receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000055">
        <property type="entry name" value="roundabout homolog 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000065">
        <property type="entry name" value="roundabout homolog 1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000026">
        <property type="entry name" value="roundabout homolog 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000008">
        <property type="entry name" value="roundabout homolog 2 isoform X2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000043">
        <property type="entry name" value="roundabout homolog 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000058">
        <property type="entry name" value="roundabout homolog 2 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
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        <property type="entry name" value="ROUNDABOUT HOMOLOG 2"/>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="RefSeq" id="XP_053536592.1"/>
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</entry>
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    <accession>A0A9F7RPI4</accession>
    <name>A0A9F7RPI4_ICTPU</name>
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        <submittedName>
            <fullName evidence="20">Zinc finger and BTB domain-containing protein 38 isoform X1</fullName>
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        <name evidence="20" type="primary">zbtb4</name>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
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    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
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        <subcellularLocation>
            <location evidence="2">Chromosome</location>
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        <subcellularLocation>
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        <property type="component" value="Chromosome 13"/>
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        <property type="entry name" value="Classic Zinc Finger"/>
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        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24408:SF58">
        <property type="entry name" value="TRANSCRIPTION FACTOR (TFIIIA), PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05150)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24408">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
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        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
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    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 16" id="KW-0863">Zinc-finger</keyword>
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        </location>
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        <source>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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        <source>
            <dbReference type="RefSeq" id="XP_053540838.1"/>
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</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RS00</accession>
    <name>A0A9F7RS00_ICTPU</name>
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            <fullName evidence="10">Short-chain specific acyl-CoA dehydrogenase, mitochondrial</fullName>
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            <fullName evidence="9">Butyryl-CoA dehydrogenase</fullName>
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    <gene>
        <name evidence="20" type="primary">acads</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Short-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Among the different mitochondrial acyl-CoA dehydrogenases, short-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 4 to 6 carbons long primary chains.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>butanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-butenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:24004"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57332"/>
            <dbReference type="ChEBI" id="CHEBI:57371"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.3.8.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:24005"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>hexanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-hexenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43464"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:62077"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:43465"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>pentanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-pentenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43456"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57389"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:86160"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:43457"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; mitochondrial fatty acid beta-oxidation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_053542593.1">
        <property type="nucleotide sequence ID" value="XM_053686618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RS00"/>
    <dbReference type="GeneID" id="100305084"/>
    <dbReference type="KEGG" id="ipu:100305084"/>
    <dbReference type="CTD" id="35"/>
    <dbReference type="OrthoDB" id="9988775at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016937">
        <property type="term" value="F:short-chain fatty acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046359">
        <property type="term" value="P:butyrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01158">
        <property type="entry name" value="SCAD_SBCAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase FadE19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000004">
        <property type="entry name" value="Acyl-CoA dehydrogenase FadE25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000001">
        <property type="entry name" value="Acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF12">
        <property type="entry name" value="ISOVALERYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016578">
        <property type="entry name" value="HsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0274">FAD</keyword>
    <keyword evidence="5 15" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="18">
        <location>
            <begin position="43"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="17">
        <location>
            <begin position="158"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="16">
        <location>
            <begin position="265"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_053542593.1"/>
        </source>
    </evidence>
    <sequence length="419" mass="45142" checksum="0B26434F36F91BD2" modified="2023-06-28" version="1">MSAEYISDEEKSKMAALFKARKALNRAVRFGKNCFTQLAGLPELHQILRQTCQDYAAKELAPIAAQLDKNHSFPAKQIQELGAMGVLAMEVPENLGGAGMDYLAYSLAVEEISRGCASTGVIVSVNNSLYLGPILKFGTEEQKQQWISPFTTGEKVGCFALSEPGNGSDAGAASTLAHQEGDEWVLNGTKAWITNCWDASAAVVFATTDKSLKHKGISAFLVPMPHPGLSLGKKEDKLGIRASSTANLILEDCRIPLVNLLGQCGMGFKIAMQTLDSGRIGIASQALGIAQAAFDCATDYAQKRIAFGAPIAKLQAIQFKLADMSVAIESARLLTWKAALLRDGKKPYSKAAAMAKLTASEAATFVSHQAIQVLGGMGYVTDMPAERHYRDARITEIYEGTSEIQRLVIANEILKEYQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RT24</accession>
    <name>A0A9F7RT24_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Ephrin-A2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">efna2a</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the ephrin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053543386.1">
        <property type="nucleotide sequence ID" value="XM_053687411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RT24"/>
    <dbReference type="GeneID" id="108277532"/>
    <dbReference type="KEGG" id="ipu:108277532"/>
    <dbReference type="CTD" id="30218"/>
    <dbReference type="OrthoDB" id="6250301at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046875">
        <property type="term" value="F:ephrin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048013">
        <property type="term" value="P:ephrin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030316">
        <property type="term" value="P:osteoclast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10425">
        <property type="entry name" value="Ephrin-A_Ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000005">
        <property type="entry name" value="Ephrin A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031328">
        <property type="entry name" value="Ephrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034252">
        <property type="entry name" value="Ephrin-A_Ecto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019765">
        <property type="entry name" value="Ephrin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001799">
        <property type="entry name" value="Ephrin_RBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11304">
        <property type="entry name" value="EPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11304:SF69">
        <property type="entry name" value="EPHRIN-A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00812">
        <property type="entry name" value="Ephrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01347">
        <property type="entry name" value="EPHRIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01299">
        <property type="entry name" value="EPHRIN_RBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51551">
        <property type="entry name" value="EPHRIN_RBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039940744" evidence="12">
        <location>
            <begin position="21"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Ephrin RBD" evidence="13">
        <location>
            <begin position="27"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="60"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_053543386.1"/>
        </source>
    </evidence>
    <sequence length="199" mass="23346" checksum="015296731FCCA3B3" modified="2023-06-28" version="1">MEVQVLVLVFAVLCMVCVWTEDRIPPSDRHAVYWNSSNTRFWHGEYTVAVNINDYLDVYCPYYEEPQPLSRMERYILFMVNHDGYTTCEHRMRGFKRWECNRPQNVDGPLRFSEKFQLFTPFSLGFEFRPGHEYYYISSPHPNHAGKPCLKLKVYVKPADGSAFESPEPFLIDRSVTVGLHLCVLMLWAAASVLMLMRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RU97</accession>
    <name>A0A9F7RU97_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Tripartite motif-containing protein 16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC108277565</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053543327.1">
        <property type="nucleotide sequence ID" value="XM_053687352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RU97"/>
    <dbReference type="GeneID" id="108277565"/>
    <dbReference type="KEGG" id="ipu:108277565"/>
    <dbReference type="OrthoDB" id="8843305at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16040">
        <property type="entry name" value="SPRY_PRY_SNTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000066">
        <property type="entry name" value="Si:ch211-208f21.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF5">
        <property type="entry name" value="E3 UBIQUITIN_ISG15 LIGASE TRIM25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="15"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="9">
        <location>
            <begin position="149"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="10">
        <location>
            <begin position="358"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="204"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053543327.1"/>
        </source>
    </evidence>
    <sequence length="546" mass="62880" checksum="5F226781ECD24398" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TB56</accession>
    <name>A0A9F7TB56_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Achaete-scute homolog 5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC128628911</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053530054.1">
        <property type="nucleotide sequence ID" value="XM_053674079.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TB56"/>
    <dbReference type="GeneID" id="128628911"/>
    <dbReference type="KEGG" id="ipu:128628911"/>
    <dbReference type="OrthoDB" id="5976910at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060429">
        <property type="term" value="P:epithelium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000038">
        <property type="entry name" value="achaete-scute homolog 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015660">
        <property type="entry name" value="MASH1/Ascl1a-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13935:SF106">
        <property type="entry name" value="ACHAETE-SCUTE COMPLEX PROTEIN T5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13935">
        <property type="entry name" value="ACHAETE-SCUTE TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="89"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="152"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_053530054.1"/>
        </source>
    </evidence>
    <sequence length="185" mass="20794" checksum="B4A48B8CEFC4620A" modified="2023-06-28" version="1">MDSAFSRPLSEHYCPYSVVSPSAGHALKHAHAQFHPARLRHTLPLLIYPRTTEPRLYDASYPGAAAPLMPYLSPFHGRFGVYECALEPAFIQKRNERERQRVKCVNQGYAKLREHLPGAAADKRMSKVETLRAAIRYIKHLQRLVEEHETSVEDVHADEEDSPLQLSITSSPVNCCDASQDSSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TDZ7</accession>
    <name>A0A9F7TDZ7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Integral membrane protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">itm2ca</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Negative regulator of amyloid-beta peptide production. May inhibit the processing of APP by blocking its access to alpha- and beta-secretase. Binding to the beta-secretase-cleaved APP C-terminal fragment is negligible, suggesting that ITM2C is a poor gamma-secretase cleavage inhibitor. May play a role in TNF-induced cell death and neuronal differentiation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with BACE1. Interacts with APP. Interacts with STMN2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Lysosome membrane</location>
            <topology evidence="14">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Membrane</location>
            <topology evidence="16">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the ITM2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053529667.1">
        <property type="nucleotide sequence ID" value="XM_053673692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TDZ7"/>
    <dbReference type="GeneID" id="108280418"/>
    <dbReference type="KEGG" id="ipu:108280418"/>
    <dbReference type="CTD" id="436822"/>
    <dbReference type="OMA" id="NCHPIRH"/>
    <dbReference type="OrthoDB" id="9982095at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001540">
        <property type="term" value="F:amyloid-beta binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042985">
        <property type="term" value="P:negative regulation of amyloid precursor protein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007084">
        <property type="entry name" value="BRICHOS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040145">
        <property type="entry name" value="ITM2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10962:SF5">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10962">
        <property type="entry name" value="INTEGRAL TRANSMEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04089">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01039">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50869">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 16" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="40"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="BRICHOS" evidence="17">
        <location>
            <begin position="122"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_053529667.1"/>
        </source>
    </evidence>
    <sequence length="257" mass="29774" checksum="B05F11740C109B1C" modified="2023-06-28" version="1">MVKISCATINGIKAEKEEREEPEHSYIPPQHVGVSRRSSLSGLCCLLIALIISTCSLVYASIYIYRYYIIPQADESQFRCNVYYEDTLKAPLKHDREELQENVNINLQNNYERISINAGNTNPHTRPATIIHDFNKGLTAYHDITLDKCYISVLNSSIVLPPRNLWKLLINVKLRTGNEASLPQTYMVQEEMVVSEQVMNTRVFGVFIHRLCEGKPTYQIRRRLTRRRIAKREEQICHSIRHFENTFVMDTLICDAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TED1</accession>
    <name>A0A9F7TED1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Semaphorin-4E-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC128628838</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053529756.1">
        <property type="nucleotide sequence ID" value="XM_053673781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TED1"/>
    <dbReference type="GeneID" id="128628838"/>
    <dbReference type="KEGG" id="ipu:128628838"/>
    <dbReference type="OrthoDB" id="9988752at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043931">
        <property type="term" value="P:ossification involved in bone maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001703">
        <property type="entry name" value="Semaphorin 4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
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    <sequence length="754" mass="84544" checksum="4CEB759BDFC338CD" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TEG8</accession>
    <name>A0A9F7TEG8_ICTPU</name>
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        <recommendedName>
            <fullName evidence="19">Voltage-dependent P/Q-type calcium channel subunit alpha-1A</fullName>
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        <alternativeName>
            <fullName evidence="20">Voltage-gated calcium channel subunit alpha Cav2.1</fullName>
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        <name evidence="29" type="primary">cacna1aa</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
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    </organism>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="28">SDA103</strain>
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    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="29">Blood</tissue>
        </source>
    </reference>
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            <text>Ca(2+)(in) = Ca(2+)(out)</text>
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            <dbReference type="ChEBI" id="CHEBI:29108"/>
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            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="23">Membrane</location>
            <topology evidence="23">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1A subfamily.</text>
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    <keyword evidence="6 23" id="KW-0107">Calcium channel</keyword>
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    <keyword evidence="2" id="KW-0813">Transport</keyword>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
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</entry>
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    <accession>A0A9F7THX1</accession>
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        <name type="scientific">Ictalurus punctatus</name>
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            <taxon>Eukaryota</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Plays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
            <location evidence="1 7">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the thymosin beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053533521.1">
        <property type="nucleotide sequence ID" value="XM_053677546.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053533522.1">
        <property type="nucleotide sequence ID" value="XM_053677547.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7THX1"/>
    <dbReference type="GeneID" id="128629433"/>
    <dbReference type="KEGG" id="ipu:128629433"/>
    <dbReference type="OrthoDB" id="2151618at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003785">
        <property type="term" value="F:actin monomer binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.520:FF:000001">
        <property type="entry name" value="Thymosin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.520">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001152">
        <property type="entry name" value="Beta-thymosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038386">
        <property type="entry name" value="Beta-thymosin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12021">
        <property type="entry name" value="THYMOSIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12021:SF10">
        <property type="entry name" value="THYMOSIN BETA-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01290">
        <property type="entry name" value="Thymosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001828">
        <property type="entry name" value="Thymosin_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00152">
        <property type="entry name" value="THY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR001828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053533521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053533522.1"/>
        </source>
    </evidence>
    <sequence length="43" mass="4961" checksum="A742884AA3FBE169" modified="2023-06-28" version="1">MADKPDVSEISHFDKSKLKKTETQEKNILPTKEVIEQEKQSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TMV7</accession>
    <name>A0A9F7TMV7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">LOC124627088</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12 13">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="2"/>
    <dbReference type="RefSeq" id="XP_053541173.1">
        <property type="nucleotide sequence ID" value="XM_053685198.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053541174.1">
        <property type="nucleotide sequence ID" value="XM_053685199.1"/>
    </dbReference>
    <dbReference type="GeneID" id="124627088"/>
    <dbReference type="KEGG" id="ipu:124627088"/>
    <dbReference type="OrthoDB" id="8962756at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.960.10">
        <property type="entry name" value="VSR Endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004868">
        <property type="entry name" value="DNA-dir_DNA_pol_B_mt/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33568">
        <property type="entry name" value="DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33568:SF3">
        <property type="entry name" value="DNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03175">
        <property type="entry name" value="DNA_pol_B_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B mitochondria/virus" evidence="10">
        <location>
            <begin position="736"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B mitochondria/virus" evidence="10">
        <location>
            <begin position="1070"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="250"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="130"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="269"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053541173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_053541174.1"/>
        </source>
    </evidence>
    <sequence length="1511" mass="174816" checksum="14F551E1E492B4A3" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TMW8</accession>
    <name>A0A9F7TMW8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">LIM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">lims1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Within the IPP (ILK-PINCH-PARVIN) complex, binds to F-actin, promoting F-actin bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053539237.1">
        <property type="nucleotide sequence ID" value="XM_053683262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TMW8"/>
    <dbReference type="GeneID" id="108270729"/>
    <dbReference type="CTD" id="3987"/>
    <dbReference type="OrthoDB" id="20689at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:cell surface receptor signaling pathway"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0045216">
        <property type="term" value="P:cell-cell junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001046">
        <property type="term" value="P:positive regulation of integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900026">
        <property type="term" value="P:positive regulation of substrate adhesion-dependent cell spreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="LIM1_PINCH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LIM2_PINCH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LIM3_PINCH"/>
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    </dbReference>
    <dbReference type="CDD" id="cd09334">
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    </dbReference>
    <dbReference type="CDD" id="cd09335">
        <property type="entry name" value="LIM5_PINCH"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000011">
        <property type="entry name" value="Lim and senescent cell antigen-like-containing"/>
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    </dbReference>
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        <property type="entry name" value="Lim and senescent cell antigen-like-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000019">
        <property type="entry name" value="Lim and senescent cell antigen-like-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000021">
        <property type="entry name" value="Lim and senescent cell antigen-like-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000062">
        <property type="entry name" value="LIM domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047944">
        <property type="entry name" value="LIMS1/2-like_LIM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017351">
        <property type="entry name" value="PINCH-1-4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047946">
        <property type="entry name" value="PINCH-1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24210:SF12">
        <property type="entry name" value="LIM AND SENESCENT CELL ANTIGEN-LIKE-CONTAINING DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24210">
        <property type="entry name" value="LIM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038003">
        <property type="entry name" value="PINCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 13 14" id="KW-0440">LIM domain</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="19"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="82"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="202"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="262"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR038003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_053539237.1"/>
        </source>
    </evidence>
    <sequence length="345" mass="39425" checksum="6ED6D7DC9F6CC90B" modified="2023-06-28" version="1">MLGVSEMTGSNMANALANAACERCKSGFAPAEKIVNSNGELYHEQCFVCAQCFQQFPEGLFYEFEGRKYCEHDFQMLFAPCCHQCGEFIIGRVIKAMNNSWHPDCFCCDICQAVLADVGFVKNAGRHLCRPCHNREKARGLGKYFCQKCHAIIDEQPLIFKNDPYHPDHFNCSNCGKELTADARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNAMGKQWHVEHFVCAKCEKPFLGHRHYERKGLAYCETHYNQLFGDVCYHCNRVIEGDVVSALNKAWCVNCFSCSTCNTKLTLKDKFVEVDLKPVCKHCYDRLPEELKRRLAKRERDTRERKKKTVAVCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TNZ5</accession>
    <name>A0A9F7TNZ5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP-dependent RNA helicase TDRD9-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC128634125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SDA103</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Blood</tissue>
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    </reference>
    <dbReference type="RefSeq" id="XP_053540362.1">
        <property type="nucleotide sequence ID" value="XM_053684387.1"/>
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    <dbReference type="GeneID" id="128634125"/>
    <dbReference type="KEGG" id="ipu:128634125"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="RBD_domain_sf"/>
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    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_053540362.1"/>
        </source>
    </evidence>
    <sequence length="186" mass="21467" checksum="91CEB9E82654E578" modified="2023-06-28" version="1">MTESPWNRLKITHTLRRRRTRGGEDELEWGKENCIQIKRIQEVAELFEDLKNRVSQFNMHISETSPSDYTSTHTQKFIVQVAIAGAFYPNCFLQGTVDEELASKELYGNDPRTTVVVCNLPPFAFLYYKQLQSLFRHCGQVKSISFDSSRAYVEFYRSSMRGSGVLPEVSLSLLLTQQRLPLHLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TQN8</accession>
    <name>A0A9F7TQN8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Multiple epidermal growth factor-like domains protein 6 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">megf6a</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053542327.1">
        <property type="nucleotide sequence ID" value="XM_053686352.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108276135"/>
    <dbReference type="CTD" id="565108"/>
    <dbReference type="OrthoDB" id="409374at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <location>
            <begin position="1"/>
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        </location>
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            <begin position="23"/>
            <end position="1554"/>
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            <end position="93"/>
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            <begin position="177"/>
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            <begin position="457"/>
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        <source>
            <dbReference type="RefSeq" id="XP_053542327.1"/>
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    <sequence length="1554" mass="167045" checksum="C470BEE5E38C1C93" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TRV2</accession>
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            <fullName evidence="8">TBC1 domain family member 13</fullName>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
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        <text evidence="6">Acts as a GTPase-activating protein for RAB35. Together with RAB35 may be involved in regulation of insulin-induced glucose transporter SLC2A4/GLUT4 translocation to the plasma membrane in adipocytes.</text>
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    <comment type="subunit">
        <text evidence="7">Interacts with RAB1A and RAB10; in a GTP-dependent manner.</text>
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            <location evidence="2">Cytoplasm</location>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
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        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 13"/>
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        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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            <begin position="35"/>
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    </feature>
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        <location>
            <begin position="148"/>
            <end position="175"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053543842.1"/>
        </source>
    </evidence>
    <sequence length="399" mass="46731" checksum="437AA3C7A1E6623D" modified="2023-06-28" version="1">MSSSYKNRIQEFKTSLSEEKIDLKALRELCFGGIPFEGGIRSLCWKILLNFLPLDQTLWDSFLKKQREIYSQFLKEMIIQPGIAKANLGLSREDVTMEDHPLNPNPESRWNNYFKDNEVLLQIDKDVRRLYPDMAFFQRPTEFPCQLILDSQNEYETLRRRVEQTTLKAQTVNRNRSGVTNVSSPGKALNLYPSNEYEVLPNGCEAHWEVVERILFIYAKLNPGIAYVQGMNEIVGPIYYTFATDPNPQWKEHAEADTFFCFTNLMSENRDNFIKSLDDSQCGITYKMESVFSMLKEKDLELYMKLEQNIKPQYFTFRWLTLLLSQEFLLPDVIRIWDSLFSDQDRFHFLILVCCAMLTLIRNQLLAGDFTTNMRLLQDYPISDVHTILIKAKELQDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3TDP8</accession>
    <name>E3TDP8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3 5">LYR motif-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LYM4A</name>
        <name evidence="5" type="synonym">lym4a</name>
        <name evidence="5" type="synonym">lyrm4</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I LYR family.</text>
    </comment>
    <dbReference type="EMBL" id="GU588478">
        <property type="protein sequence ID" value="ADO28434.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187277.1">
        <property type="nucleotide sequence ID" value="NM_001200348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TDP8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000481"/>
    <dbReference type="GeneID" id="100528078"/>
    <dbReference type="KEGG" id="ipu:100528078"/>
    <dbReference type="CTD" id="103909737"/>
    <dbReference type="GeneTree" id="ENSGT00940000157289"/>
    <dbReference type="OMA" id="YTTDKLV"/>
    <dbReference type="OrthoDB" id="275715at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990221">
        <property type="term" value="C:L-cysteine desulfurase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20264">
        <property type="entry name" value="Complex1_LYR_LYRM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045297">
        <property type="entry name" value="Complex1_LYR_LYRM4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051522">
        <property type="entry name" value="ISC_assembly_LYR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13166:SF7">
        <property type="entry name" value="LYR MOTIF-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13166">
        <property type="entry name" value="PROTEIN C6ORF149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="2">
        <location>
            <begin position="8"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADO28434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="NP_001187277.1"/>
        </source>
    </evidence>
    <sequence length="91" mass="10778" checksum="C757735408560D67" modified="2011-01-11" version="1">MAAPSRTQVLSLYRMLLKESNKFPTYNYRTYALRRVRDAFRENRNVEDPKVLDQLLNKARDNLAIIQRQVVIGRLYEVQRTVVEAQSGKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3TEW8</accession>
    <name>E3TEW8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8 10">Fam183a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">F183A</name>
        <name evidence="10" type="synonym">f183a</name>
        <name evidence="10" type="synonym">fam183a</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CFAP144 family.</text>
    </comment>
    <dbReference type="EMBL" id="GU588901">
        <property type="protein sequence ID" value="ADO28854.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187587.1">
        <property type="nucleotide sequence ID" value="NM_001200658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TEW8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007952"/>
    <dbReference type="GeneID" id="100528388"/>
    <dbReference type="KEGG" id="ipu:100528388"/>
    <dbReference type="CTD" id="440585"/>
    <dbReference type="OrthoDB" id="446290at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097546">
        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029214">
        <property type="entry name" value="CFAP144"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33865">
        <property type="entry name" value="PROTEIN FAM183B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33865:SF3">
        <property type="entry name" value="PROTEIN FAM183B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14886">
        <property type="entry name" value="FAM183"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADO28854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="NP_001187587.1"/>
        </source>
    </evidence>
    <sequence length="147" mass="17645" checksum="E9ADBE2A107470FE" modified="2011-01-11" version="1">MKRRQQAETTMAAKTKAKEKEPVDIVHQNAIHVETIQKEKRCEKLYTEFTINPFKKLHILPDGPMSRKMHEEVEEDPTFLKRFHEAQSEPRKKYTRPMTTSQEIGWISNPLITADHEDRRLNFRRHKSEITKHMEAAWRLKEKNQNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3TFB8</accession>
    <name>E3TFB8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Calumenin</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="12">Reticulocalbin-3 precursor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CALU</name>
        <name evidence="12" type="synonym">calu</name>
        <name evidence="12" type="synonym">rcn3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CREC family.</text>
    </comment>
    <dbReference type="EMBL" id="GU589052">
        <property type="protein sequence ID" value="ADO29004.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187699.1">
        <property type="nucleotide sequence ID" value="NM_001200770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TFB8"/>
    <dbReference type="GeneID" id="100528500"/>
    <dbReference type="KEGG" id="ipu:100528500"/>
    <dbReference type="CTD" id="57333"/>
    <dbReference type="GeneTree" id="ENSGT01010000222360"/>
    <dbReference type="OrthoDB" id="293868at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16226">
        <property type="entry name" value="EFh_CREC_Calumenin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000109">
        <property type="entry name" value="calumenin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000110">
        <property type="entry name" value="calumenin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827">
        <property type="entry name" value="RETICULOCALBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827:SF91">
        <property type="entry name" value="RETICULOCALBIN 3, EF-HAND CALCIUM BINDING DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5011323927" evidence="8 12">
        <location>
            <begin position="16"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="63"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="99"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="148"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="185"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="262"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADO29004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="NP_001187699.1"/>
        </source>
    </evidence>
    <sequence length="313" mass="36609" checksum="D95EEE446C919306" modified="2011-01-11" version="1">MQLFILAVVLALGLAVPPQEKRVHHQRDLSDHAHHDTQGYQYDHDAFLGKEEAKTFDQLTPEESKARLGKIVERIDTDKDGFVSHAELHYWIKHRQRRYIEENVDKNWKEYDQNKDGKISWIEYKNTTYGTYLDDESEDLEDRESYKAMHARDQRRFKMADKDGDGIATREEFTAFLHPEEFDYMKGLVVQETMEDIDKNGDGKINLEEYIGDMFTPEEGESEPEWVATERKHFSEYRDANKDGFLDADEVAHWILPNDVDHTDNEAKHLIHETDKNNDGLLSVSEIMDELDVIKISTITDYGSLLQTEHDEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2026-01-28" version="114" xmlns="https://uniprot.org/uniprot">
    <accession>O73853</accession>
    <name>CP17A_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName>Steroid 17-alpha-hydroxylase/17,20 lyase</fullName>
            <ecNumber evidence="2">1.14.14.19</ecNumber>
            <ecNumber evidence="2">1.14.14.32</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>17-alpha-hydroxyprogesterone aldolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>CYPXVII</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Cytochrome P450 17A1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Cytochrome P450-C17</fullName>
            <shortName>Cytochrome P450c17</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cyp17a1</name>
        <name type="synonym">cyp17</name>
    </gene>
    <organism>
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="1998-05" db="EMBL/GenBank/DDBJ databases">
            <title>Isolation and heterologous expression of the cDNA encoding the cytochrome P450 17-hydroxylase from the channel catfish (Ictalurus punctatus).</title>
            <authorList>
                <person name="Trant J.M."/>
                <person name="Berard C."/>
                <person name="Byrne B.J."/>
                <person name="Wunder J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [MRNA]</scope>
        <source>
            <tissue>Ovary</tissue>
        </source>
    </reference>
    <comment type="function">
        <text>Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a C21-steroid + reduced [NADPH--hemoprotein reductase] + O2 = a 17alpha-hydroxy-C21-steroid + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:65760"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:61313"/>
            <dbReference type="ChEBI" id="CHEBI:138141"/>
            <dbReference type="EC" id="1.14.14.19"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>17alpha-hydroxyprogesterone + reduced [NADPH--hemoprotein reductase] + O2 = androst-4-ene-3,17-dione + acetate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14753"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16422"/>
            <dbReference type="ChEBI" id="CHEBI:17252"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.32"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>17alpha-hydroxypregnenolone + reduced [NADPH--hemoprotein reductase] + O2 = 3beta-hydroxyandrost-5-en-17-one + acetate + oxidized [NADPH--hemoprotein reductase] + H2O + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:50244"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:28689"/>
            <dbReference type="ChEBI" id="CHEBI:28750"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.32"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text>Lipid metabolism; steroid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.19" evidence="2"/>
    <dbReference type="EC" id="1.14.14.32" evidence="2"/>
    <dbReference type="EMBL" id="AF063837">
        <property type="protein sequence ID" value="AAC16551.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187242.1">
        <property type="nucleotide sequence ID" value="NM_001200313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O73853"/>
    <dbReference type="SMR" id="O73853"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000036506"/>
    <dbReference type="GeneID" id="100313515"/>
    <dbReference type="KEGG" id="ipu:100313515"/>
    <dbReference type="CTD" id="105601846"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="UniPathway" id="UPA00062"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004508">
        <property type="term" value="F:steroid 17-alpha-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042446">
        <property type="term" value="P:hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042448">
        <property type="term" value="P:progesterone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006694">
        <property type="term" value="P:steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd20673">
        <property type="entry name" value="CYP17A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000002">
        <property type="entry name" value="Cytochrome P450 1A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24289:SF14">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 17, SUBFAMILY A, POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24289">
        <property type="entry name" value="STEROID 17-ALPHA-HYDROXYLASE/17,20 LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword id="KW-0349">Heme</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0503">Monooxygenase</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0755">Steroidogenesis</keyword>
    <feature type="chain" id="PRO_0000051945" description="Steroid 17-alpha-hydroxylase/17,20 lyase">
        <location>
            <begin position="1"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="1">
        <location>
            <position position="445"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P05093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="514" mass="58134" checksum="7CDF5CC0F6982053" modified="1998-08-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5U722</accession>
    <name>W5U722_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="2 4">Protein OSCP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">Oscp1</name>
        <name evidence="4" type="synonym">oscp1b</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT406832">
        <property type="protein sequence ID" value="AHH37741.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017330982.1">
        <property type="nucleotide sequence ID" value="XM_017475493.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U722"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022938"/>
    <dbReference type="GeneID" id="108269545"/>
    <dbReference type="KEGG" id="ipu:108269545"/>
    <dbReference type="CTD" id="100334608"/>
    <dbReference type="OMA" id="GTMFNKR"/>
    <dbReference type="OrthoDB" id="2157380at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019332">
        <property type="entry name" value="OSCP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21439">
        <property type="entry name" value="OXIDORED-NITRO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21439:SF0">
        <property type="entry name" value="PROTEIN OSCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10188">
        <property type="entry name" value="Oscp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="356"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AHH37741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017330982.1"/>
        </source>
    </evidence>
    <sequence length="380" mass="42849" checksum="516DB4C55387EFBD" modified="2014-04-16" version="1">MSTRTLPLVFINLGGEMLYILDQRLRAQNIPTDKAKKVMNDIITTMFNKKFLEELFKPQELYSKKALRTVFDRLAHASIMRLNQASMDKLYDLMTMAFKYQVLLCPRPKDILLVTFNHMDAIKEFVRDTPSILSQVDETYTQLIEIYTPLSCGEFQLIRQTLLIFFQDMHIRVSIFLKDKVQNSNGRFVLPTSGPVPYGTHSPGLIRMLSCNGEEVKRLQFHDGGNYTAALREGSFELFGDRVIKLGTNMYSVSRPVETHMSGTSKISSQHTKVNTIPNPLAKEELNLLAKLMGGLEVRKPSNTDSGFRVNLFATDEEEEEALISRPAELSYGVINIQATKDNLANAELAKIMGEFTESDEPVPSSSSKGDDLLAMMDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5U8X0</accession>
    <name>W5U8X0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Actin-like protein 6A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">BRG1-associated factor 53A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">ACTL6A</name>
        <name evidence="25" type="synonym">actl6a</name>
    </gene>
    <organism evidence="23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">SDA103</strain>
        </source>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="19">Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 22">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="JT408112">
        <property type="protein sequence ID" value="AHH38240.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017335325.1">
        <property type="nucleotide sequence ID" value="XM_017479836.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U8X0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013405"/>
    <dbReference type="GeneID" id="108271902"/>
    <dbReference type="KEGG" id="ipu:108271902"/>
    <dbReference type="CTD" id="86"/>
    <dbReference type="GeneTree" id="ENSGT00940000156305"/>
    <dbReference type="OMA" id="MTEAPWN"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071564">
        <property type="term" value="C:npBAF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045739">
        <property type="term" value="P:positive regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033044">
        <property type="term" value="P:regulation of chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13395">
        <property type="entry name" value="ASKHA_NBD_Arp4_ACTL6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000050">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.36.70:FF:000004">
        <property type="entry name" value="Actin-like 6A, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000009">
        <property type="entry name" value="Actin-like 6A, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000375">
        <property type="entry name" value="Actin-related protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.36.70">
        <property type="entry name" value="Actin, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="14" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="16" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="17" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="10" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0558">Oxidation</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00038582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00072655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00076522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="AHH38240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_017335325.1"/>
        </source>
    </evidence>
    <sequence length="429" mass="47414" checksum="4F95F77F2F36FC93" modified="2014-04-16" version="1">MSGGVYGGDEVGALVFDIGSYSVRAGYAGEDCPKADFPTVIGVTVDREDGTTPMETDGDKGKQSGTTYYIDTNQLRVPRENMEVMSPLKNGMIEDWDSFQAILDHTYRMHFKSEPSLHPVLMSEASWNTRAKREKLTELMFEHYNIPAFFLCKTAVLSAFANGRSTGLVLDSGATHTTAIPVHDGYVLQQGIVKSPLAGDFMSMQCRELFQELSVEIIPTYMIASKDSVREGSPASWKKKEKLPQVTRSWHNYMCNTVIQDFQASVLQVSDSPYDEQVAAQMPTVHYELPNGYNCDFGAERLKIPEGLFDPSNAKGLSGNTMLGVGHVVTTSVGMCDVDIRPGLYGSVIVTGGNTLIQGFTDRLNRELSQKTPPSMRLKLIANNTTLERRFSAWIGGSILASLGTFQQMWISKQEYEEGGKQCVERKCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5U970</accession>
    <name>W5U970_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3 5">Transmembrane protein 240</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TMEM240</name>
        <name evidence="5" type="synonym">tmem240a</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT408496">
        <property type="protein sequence ID" value="AHH38355.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017341977.1">
        <property type="nucleotide sequence ID" value="XM_017486488.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U970"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032371"/>
    <dbReference type="GeneID" id="108275582"/>
    <dbReference type="KEGG" id="ipu:108275582"/>
    <dbReference type="CTD" id="568378"/>
    <dbReference type="GeneTree" id="ENSGT00400000023987"/>
    <dbReference type="OMA" id="HHSQYDE"/>
    <dbReference type="OrthoDB" id="9922101at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027947">
        <property type="entry name" value="TMEM240"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28666">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28666:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15207">
        <property type="entry name" value="TMEM240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1 3 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540076" evidence="2">
        <location>
            <begin position="21"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AHH38355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017341977.1"/>
        </source>
    </evidence>
    <sequence length="175" mass="20412" checksum="A807282EFFDD3B85" modified="2014-04-16" version="1">MHMITTTMIFMILGASVVMAIACLMDMNALLDRFHNYILPHLRGEDRVCHCNCGRHHVHYVIPYDGDHSLVDSSENYFVSDSVTKQEMDLMLGLLLGFCISWLLLWLDGALHCAVRAWRASRYYDTPSWSWLPQFCNLRDLRRRTQLRQLEDSSGNMVHIKQKLYHNGHPSPRHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5U9G6</accession>
    <name>W5U9G6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 1</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="4">BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Kctd10</name>
        <name evidence="6" type="synonym">kctd13</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BACURD family.</text>
    </comment>
    <dbReference type="EMBL" id="JT409358">
        <property type="protein sequence ID" value="AHH38646.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017345425.1">
        <property type="nucleotide sequence ID" value="XM_017489936.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U9G6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000002125"/>
    <dbReference type="GeneID" id="108277315"/>
    <dbReference type="KEGG" id="ipu:108277315"/>
    <dbReference type="CTD" id="253980"/>
    <dbReference type="GeneTree" id="ENSGT00950000183143"/>
    <dbReference type="OMA" id="GCRIPMI"/>
    <dbReference type="OrthoDB" id="2333377at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021952">
        <property type="term" value="P:central nervous system projection neuron axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035024">
        <property type="term" value="P:negative regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061351">
        <property type="term" value="P:neural precursor cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000046">
        <property type="entry name" value="BTB/POZ domain-containing protein KCTD7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045068">
        <property type="entry name" value="BACURD1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING ADAPTER FOR CUL3-MEDIATED RHOA DEGRADATION PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145:SF26">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING ADAPTER FOR CUL3-MEDIATED RHOA DEGRADATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="37"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="282"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AHH38646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017345425.1"/>
        </source>
    </evidence>
    <sequence length="330" mass="36543" checksum="A924C6B344A31CAD" modified="2014-04-16" version="1">MSAEASGENVVPDPSCAPPSPSHGVEKKVGPGLAGSKYVKLNVGGSLHYTTVQTLSKEDSLLRRICDGSVEVTIDSEGWVVLDRCGRHFSLVLNFLRDGTVPLPESQRELEEVLKEAQYYRLQGLIQHCLSTLQNRSEVYEGGCRIPMITSAKEEQRMIATCKKPVVKLQNNRGNNKYSYTSNSDDNLLKNIELFDKLGLRFNGRVLFMKDVLGDEICCWSFYGDGRKIAEVCCTSIVYATEKKQTKVEFPEARIFEETLNILIYENGRGSGHGGMGLLDSSGVSSPGVGQSEEDGAGVGGGDRRVRRIHVRRHIMHDERGHGQQTVYKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5UB82</accession>
    <name>W5UB82_ICTPU</name>
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        <alternativeName>
            <fullName evidence="9">Rac/Cdc42 guanine nucleotide exchange factor 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ARHGEF9</name>
        <name evidence="19" type="synonym">arhgef9a</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Acts as a guanine nucleotide exchange factor (GEF) for CDC42. Promotes formation of GPHN clusters.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with GPHN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Postsynaptic density</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="mRNA"/>
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    <dbReference type="GeneTree" id="ENSGT00940000160622"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
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        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="PH_Collybistin_ASEF"/>
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    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Rho guanine nucleotide exchange factor 9"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Dbl homology (DH) domain"/>
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    </dbReference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    </dbReference>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARHGEF9_SH3"/>
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        <property type="entry name" value="DH_dom"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DBL homology domain (DH-domain)"/>
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    </dbReference>
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        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
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        <location>
            <begin position="10"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="16">
        <location>
            <begin position="113"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="15">
        <location>
            <begin position="328"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011232"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00017486"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
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        <source>
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            <dbReference type="EMBL" id="AHH39218.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017315404.1"/>
        </source>
    </evidence>
    <sequence length="517" mass="60579" checksum="A9411BA4D62D71E4" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5UBL5</accession>
    <name>W5UBL5_ICTPU</name>
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        <recommendedName>
            <fullName evidence="3">Activated RNA polymerase II transcriptional coactivator p15</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">SUB1 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SUB1</name>
        <name evidence="15" type="synonym">sub1a</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transcriptional coactivator PC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT410940">
        <property type="protein sequence ID" value="AHH39240.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017344703.1">
        <property type="nucleotide sequence ID" value="XM_017489214.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UBL5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000036373"/>
    <dbReference type="GeneID" id="108276987"/>
    <dbReference type="KEGG" id="ipu:108276987"/>
    <dbReference type="CTD" id="553561"/>
    <dbReference type="GeneTree" id="ENSGT00390000008802"/>
    <dbReference type="OMA" id="VTINEFR"/>
    <dbReference type="OrthoDB" id="2505440at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.31.10:FF:000001">
        <property type="entry name" value="Activated RNA polymerase II transcriptional coactivator p15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.31.10">
        <property type="entry name" value="Transcriptional Coactivator Pc4, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003173">
        <property type="entry name" value="PC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009044">
        <property type="entry name" value="ssDNA-bd_transcriptional_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045125">
        <property type="entry name" value="Sub1/Tcp4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13215">
        <property type="entry name" value="RNA POLYMERASE II TRANSCRIPTIONAL COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02229">
        <property type="entry name" value="PC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54447">
        <property type="entry name" value="ssDNA-binding transcriptional regulator domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcriptional coactivator p15 (PC4) C-terminal" evidence="12">
        <location>
            <begin position="64"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="43"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AHH39240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017344703.1"/>
        </source>
    </evidence>
    <sequence length="127" mass="14331" checksum="F6466FE2C7748880" modified="2014-04-16" version="1">MPKSKEVLSSTSTSDSDSEADTKAKRKKQMTEKPAKKQKSGESSKTSSAPKSGGSSANKKDNMFQIGKMRYVSVREFKGKVLIDIREYWMDQEGEMKPGKKGISLNPEQWNQLKEQIDDIDDALRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>W5UCG3</accession>
    <accession>A0A2D0SAF4</accession>
    <name>W5UCG3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Receptor expression-enhancing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">reep3</name>
        <name evidence="16" type="synonym">reep3b</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Microtubule-binding protein required to ensure proper cell division and nuclear envelope reassembly by sequestering the endoplasmic reticulum away from chromosomes during mitosis. Probably acts by clearing the endoplasmic reticulum membrane from metaphase chromosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the DP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT405910">
        <property type="protein sequence ID" value="AHH37377.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017339712.2">
        <property type="nucleotide sequence ID" value="XM_017484223.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UCG3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000010109"/>
    <dbReference type="GeneID" id="108274211"/>
    <dbReference type="KEGG" id="ipu:108274211"/>
    <dbReference type="CTD" id="393130"/>
    <dbReference type="GeneTree" id="ENSGT00940000158794"/>
    <dbReference type="OrthoDB" id="434647at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071782">
        <property type="term" value="C:endoplasmic reticulum tubular network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071786">
        <property type="term" value="P:endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF39">
        <property type="entry name" value="RECEPTOR EXPRESSION-ENHANCING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="167"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AHH37377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017339712.2"/>
        </source>
    </evidence>
    <sequence length="262" mass="29717" checksum="44BD7C9F25D90BB4" modified="2014-04-16" version="1">MVSWIISRAVVLLFGTLYPAYYSYKAVKTKNVKEYVRWMMYWIVFALYTVAETIADLSVAWFPLYYEIKIACVIWLVSPYTRGASVIYRKILHPLLTSKEREIDDYIVQAKERSYETMLHFGKQGLSIAATAAVSAAVKGQGAITDKLRSFSMVDLTQIPHDDAVGEQYSSYSSSNPARRALASSQHDGAGYYEDAEEERGGNDSDTVFSEDEAVTQRGLRRSQSTKSTRSKHRKDFRYGSLKIKGRRRPALNAMTYTSTDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>W5UCY6</accession>
    <name>W5UCY6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">E3 ubiquitin-protein ligase Midline-1</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">RING finger protein Midline-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">RING-type E3 ubiquitin transferase Midline-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Tripartite motif-containing protein 18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">MID1</name>
        <name evidence="29 30" type="synonym">mid1</name>
    </gene>
    <organism evidence="27">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="27">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="28" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">SDA103</strain>
        </source>
    </reference>
    <reference evidence="29 30" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="29 30">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
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        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRIM/RBCC family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
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        <property type="protein sequence ID" value="AHH39803.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017313548.1">
        <property type="nucleotide sequence ID" value="XM_017458059.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047007131.1">
        <property type="nucleotide sequence ID" value="XM_047151175.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UCY6"/>
    <dbReference type="GeneID" id="108258982"/>
    <dbReference type="KEGG" id="ipu:108258982"/>
    <dbReference type="CTD" id="4281"/>
    <dbReference type="OMA" id="IMANYCK"/>
    <dbReference type="OrthoDB" id="9049620at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19836">
        <property type="entry name" value="Bbox1_MID1_C-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19822">
        <property type="entry name" value="Bbox2_MID1_C-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000153">
        <property type="entry name" value="Probable E3 ubiquitin-protein ligase MID2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003649">
        <property type="entry name" value="Bbox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017903">
        <property type="entry name" value="COS_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050617">
        <property type="entry name" value="E3_ligase_FN3/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047095">
        <property type="entry name" value="MID1_Bbox1_Zfn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027727">
        <property type="entry name" value="MID1_Bbox2_Zfn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040859">
        <property type="entry name" value="Midline-1_COS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099:SF23">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MIDLINE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22586">
        <property type="entry name" value="ANCHR-like_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18568">
        <property type="entry name" value="COS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00502">
        <property type="entry name" value="BBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51262">
        <property type="entry name" value="COS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="12 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="22">
        <location>
            <begin position="10"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="23">
        <location>
            <begin position="180"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="COS" evidence="26">
        <location>
            <begin position="330"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="25">
        <location>
            <begin position="391"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="24">
        <location>
            <begin position="476"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00032675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00033203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="AHH39803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_017313548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="RefSeq" id="XP_047007131.1"/>
        </source>
    </evidence>
    <sequence length="677" mass="75767" checksum="D5AF49C3CC1C5D78" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5UFL6</accession>
    <name>W5UFL6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ornithine decarboxylase</fullName>
            <ecNumber evidence="9">4.1.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">Odc1</name>
        <name evidence="18" type="synonym">LOC108269971</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the first and rate-limiting step of polyamine biosynthesis that converts ornithine into putrescine, which is the precursor for the polyamines, spermidine and spermine. Polyamines are essential for cell proliferation and are implicated in cellular processes, ranging from DNA replication to apoptosis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-ornithine + H(+) = putrescine + CO2</text>
            <dbReference type="Rhea" id="RHEA:22964"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="4.1.1.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amine and polyamine biosynthesis; putrescine biosynthesis via L-ornithine pathway; putrescine from L-ornithine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the Orn/Lys/Arg decarboxylase class-II family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.17" evidence="9"/>
    <dbReference type="EMBL" id="JT408883">
        <property type="protein sequence ID" value="AHH38482.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017331656.1">
        <property type="nucleotide sequence ID" value="XM_017476167.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UFL6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012652"/>
    <dbReference type="GeneID" id="108269971"/>
    <dbReference type="KEGG" id="ipu:108269971"/>
    <dbReference type="OMA" id="AYCRSMA"/>
    <dbReference type="OrthoDB" id="5034579at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004586">
        <property type="term" value="F:ornithine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033387">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00622">
        <property type="entry name" value="PLPDE_III_ODC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.37.10:FF:000005">
        <property type="entry name" value="Ornithine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.10:FF:000006">
        <property type="entry name" value="Ornithine decarboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="entry name" value="Lyase, Ornithine Decarboxylase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022643">
        <property type="entry name" value="De-COase2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022657">
        <property type="entry name" value="De-COase2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022653">
        <property type="entry name" value="De-COase2_pyr-phos_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002433">
        <property type="entry name" value="Orn_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11482">
        <property type="entry name" value="ARGININE/DIAMINOPIMELATE/ORNITHINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11482:SF42">
        <property type="entry name" value="ORNITHINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00278">
        <property type="entry name" value="Orn_DAP_Arg_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01182">
        <property type="entry name" value="ORNDCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="Alanine racemase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00878">
        <property type="entry name" value="ODR_DC_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00879">
        <property type="entry name" value="ODR_DC_2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="5 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn/DAP/Arg decarboxylase 2 C-terminal" evidence="14">
        <location>
            <begin position="38"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Orn/DAP/Arg decarboxylase 2 N-terminal" evidence="15">
        <location>
            <begin position="43"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="12">
        <location>
            <position position="359"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="12">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR600183-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AHH38482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017331656.1"/>
        </source>
    </evidence>
    <sequence length="459" mass="50453" checksum="70328BDE6CC7FDB7" modified="2014-04-16" version="1">MTSFSDFEFTLLEEGFCARDIVEQKIRESTSSDDKDAFYVADLGDILKKHLRWARALPRVKPFYAVKCNDGLAVVETLAMLGTGFDCASKTEIQLVQSLGVEPSRIIYANPCKQVSQIKYASAHGVQMMTFDSEVELMKVARSHDNAKLVLRIATDDSKAVCRLSVKFGATLKSSRLLLERAKELGLDIIGVSFHVGSGCTDPATYTQAIADARYVFDMGAELGYNMTLLDIGGGFPGSDDSKLKFEEITSVINPALDKYFPVDCGVRIIAEPGRFYAASAYTLAVNIIAKKVNMKEQSGSDDEDDGASDRTLMYYVNDGVYGSFNCILFDHAHVTPIVHKKPKPDEHMYPCSIWGPTCDGLDRIVELCTLPDRQVGDWLLFENMGAYTVAASSTFNGFQKPDIYYVMSRSAWQRMQQIHAQGMPALVEEQSPGNMPGHCGCGSTLELPAKPCATSVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5UIH3</accession>
    <name>W5UIH3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5 7">N-alpha-acetyltransferase 15, NatA auxiliary subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">NAA15</name>
        <name evidence="7" type="synonym">naa15b</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT415773">
        <property type="protein sequence ID" value="AHH41816.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017319921.1">
        <property type="nucleotide sequence ID" value="XM_017464432.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UIH3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000014244"/>
    <dbReference type="GeneID" id="108263533"/>
    <dbReference type="CTD" id="334099"/>
    <dbReference type="OMA" id="INPDHPW"/>
    <dbReference type="OrthoDB" id="10263032at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1010:FF:000001">
        <property type="entry name" value="N-alpha-acetyltransferase 15, NatA auxiliary subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1040:FF:000001">
        <property type="entry name" value="N-alpha-acetyltransferase 15, NatA auxiliary subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021183">
        <property type="entry name" value="NatA_aux_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767:SF6">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 15, NATA AUXILIARY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12569">
        <property type="entry name" value="NatA_aux_su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000422">
        <property type="entry name" value="N-terminal-AcTrfase-A_aux_su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="80"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="224"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="595"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="595"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="606"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AHH41816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017319921.1"/>
        </source>
    </evidence>
    <sequence length="865" mass="100588" checksum="DBCA57ECCAAEBDA8" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5UIU5</accession>
    <name>W5UIU5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4 6">Fas-activated serine/threonine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">FASTK</name>
        <name evidence="6" type="synonym">fastk</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT416529">
        <property type="protein sequence ID" value="AHH42069.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017327735.1">
        <property type="nucleotide sequence ID" value="XM_017472246.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UIU5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003566"/>
    <dbReference type="GeneID" id="108267814"/>
    <dbReference type="KEGG" id="ipu:108267814"/>
    <dbReference type="CTD" id="10922"/>
    <dbReference type="GeneTree" id="ENSGT01030000234607"/>
    <dbReference type="OMA" id="PSDYYCS"/>
    <dbReference type="OrthoDB" id="8881103at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035770">
        <property type="term" value="C:ribonucleoprotein granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000963">
        <property type="term" value="P:mitochondrial RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044528">
        <property type="term" value="P:regulation of mitochondrial mRNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="FAST_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050870">
        <property type="entry name" value="FAST_kinase"/>
    </dbReference>
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        <property type="entry name" value="FAST_Leu-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013584">
        <property type="entry name" value="RAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228:SF4">
        <property type="entry name" value="FAS-ACTIVATED SERINE_THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228">
        <property type="entry name" value="FAST LEU-RICH DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06743">
        <property type="entry name" value="FAST_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08368">
        <property type="entry name" value="FAST_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08373">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00952">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51286">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RAP" evidence="3">
        <location>
            <begin position="844"/>
            <end position="902"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AHH42069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017327735.1"/>
        </source>
    </evidence>
    <sequence length="909" mass="101111" checksum="16C2CF47A5E40E75" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5UJ49</accession>
    <name>W5UJ49_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16 18">Protocadherin-18</fullName>
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    </protein>
    <gene>
        <name evidence="16" type="primary">PCDH18</name>
        <name evidence="18" type="synonym">pcdh18b</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT410447">
        <property type="protein sequence ID" value="AHH39064.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JT415493">
        <property type="protein sequence ID" value="AHH41715.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017330561.1">
        <property type="nucleotide sequence ID" value="XM_017475072.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UJ49"/>
    <dbReference type="GeneID" id="108269290"/>
    <dbReference type="KEGG" id="ipu:108269290"/>
    <dbReference type="CTD" id="562031"/>
    <dbReference type="GeneTree" id="ENSGT00940000156295"/>
    <dbReference type="OrthoDB" id="6252479at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000003">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF306">
        <property type="entry name" value="PROTOCADHERIN 18B ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
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    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540338" evidence="14">
        <location>
            <begin position="31"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="710"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="36"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="149"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="258"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="380"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="477"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="608"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="781"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="876"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="952"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="876"/>
            <end position="885"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AHH41715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017330561.1"/>
        </source>
    </evidence>
    <sequence length="1162" mass="126572" checksum="CE9BE4C233162AA9" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5UJ66</accession>
    <name>W5UJ66_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="19 21">Feline leukemia virus subgroup C receptor-related protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">Flvcr2</name>
        <name evidence="21" type="synonym">flvcr2b</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Choline uniporter that specifically mediates choline uptake at the blood-brain-barrier. Responsible for the majority of choline uptake across the blood-brain-barrier from the circulation into the brain. Choline, a nutrient critical for brain development, is a precursor of phosphatidylcholine, as well as betaine. Also mediates transport of ethanolamine. Choline and ethanolamine transport is not coupled with proton transport and is exclusively driven by the choline gradient across the plasma membrane. Also acts as a heme b transporter.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>choline(out) = choline(in)</text>
            <dbReference type="Rhea" id="RHEA:32751"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ethanolamine(in) = ethanolamine(out)</text>
            <dbReference type="Rhea" id="RHEA:32747"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>heme b(in) = heme b(out)</text>
            <dbReference type="Rhea" id="RHEA:75443"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the major facilitator superfamily. Feline leukemia virus subgroup C receptor (TC 2.A.1.28.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="JT416395">
        <property type="protein sequence ID" value="AHH42041.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017306456.1">
        <property type="nucleotide sequence ID" value="XM_017450967.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UJ66"/>
    <dbReference type="GeneID" id="108255188"/>
    <dbReference type="KEGG" id="ipu:108255188"/>
    <dbReference type="CTD" id="569329"/>
    <dbReference type="OrthoDB" id="422206at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015220">
        <property type="term" value="F:choline transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015232">
        <property type="term" value="F:heme transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097037">
        <property type="term" value="P:heme export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000101">
        <property type="entry name" value="feline leukemia virus subgroup C receptor-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000092">
        <property type="entry name" value="Feline leukemia virus subgroup C receptor-related protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049680">
        <property type="entry name" value="FLVCR1-2_SLC49-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10924:SF3">
        <property type="entry name" value="HEME TRANSPORTER FLVCR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10924">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="19 21" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="349"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="411"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="437"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="18">
        <location>
            <begin position="57"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="468"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="468"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AHH42041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017306456.1"/>
        </source>
    </evidence>
    <sequence length="492" mass="54391" checksum="377DDA983506B696" modified="2014-04-16" version="1">MGEQRNWDGEADENCFSNSETQKESQVQVFSEPSASARLPESRDQMETHLYKRRWLIVILFSSYSLCNSYQWIQYGIINNIFMKFYNVDSFTIDWMSMIYMLTYIPLIFPVTWLLEKKGLRVVALAATALNCAGTWIKVASARPDLFPVTFLGQVTCSVAQVFILGMPSRIASVWFGSNEVSTACSIGVFGNQLGIAIGFLVPPILVPNVEDMDELANHIQVMFYITAGVATFLFILVIMVFQERPALPPSQSQASARLIPTESYSYLSSILRLLRNTPFILLIISYGLNVGCFYAVGTLLNQMIIKHYPGEEVNAGRIGLTIIIAGMVSSLICGIWLDRTKMYKQTTLAVYTMSLVGLAVYSFTLDLGHLWVVFITAGALGFFMTGYLPLGFEFAVELTYPESEGTSSGLLNCSAQVFGIIFTICQGKIINTFGTLAGNLFLCAFLITGTVLTGFIKSELRRQRANQLAKTPETSDGSHANSTAVGPATRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5UJM8</accession>
    <name>W5UJM8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein polybromo-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">PBRM1</name>
        <name evidence="18" type="synonym">pbrm1l</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT417172">
        <property type="protein sequence ID" value="AHH42282.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017335518.1">
        <property type="nucleotide sequence ID" value="XM_017480029.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000035423"/>
    <dbReference type="GeneID" id="108271990"/>
    <dbReference type="KEGG" id="ipu:108271990"/>
    <dbReference type="CTD" id="445141"/>
    <dbReference type="OMA" id="SKHEYPQ"/>
    <dbReference type="OrthoDB" id="10009055at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04717">
        <property type="entry name" value="BAH_polybromo"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05524">
        <property type="entry name" value="Bromo_polybromo_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05517">
        <property type="entry name" value="Bromo_polybromo_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05520">
        <property type="entry name" value="Bromo_polybromo_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05518">
        <property type="entry name" value="Bromo_polybromo_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05515">
        <property type="entry name" value="Bromo_polybromo_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05526">
        <property type="entry name" value="Bromo_polybromo_VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21984">
        <property type="entry name" value="HMG-box_PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000009">
        <property type="entry name" value="Protein polybromo-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000011">
        <property type="entry name" value="Protein polybromo-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000013">
        <property type="entry name" value="Protein polybromo-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000006">
        <property type="entry name" value="protein polybromo-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000010">
        <property type="entry name" value="protein polybromo-1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000015">
        <property type="entry name" value="protein polybromo-1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.490:FF:000002">
        <property type="entry name" value="protein polybromo-1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.490:FF:000003">
        <property type="entry name" value="protein polybromo-1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037968">
        <property type="entry name" value="PBRM1_BD5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037382">
        <property type="entry name" value="Rsc/polybromo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16062:SF19">
        <property type="entry name" value="PROTEIN POLYBROMO-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16062">
        <property type="entry name" value="SWI/SNF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 10" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="13">
        <location>
            <begin position="63"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="13">
        <location>
            <begin position="202"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="13">
        <location>
            <begin position="371"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="13">
        <location>
            <begin position="506"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="13">
        <location>
            <begin position="645"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="domain" description="BAH" evidence="15">
        <location>
            <begin position="931"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="domain" description="BAH" evidence="15">
        <location>
            <begin position="1128"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="domain" description="HMG box" evidence="14">
        <location>
            <begin position="1350"/>
            <end position="1418"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="11">
        <location>
            <begin position="1350"/>
            <end position="1418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="154"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="461"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="589"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
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            <begin position="888"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1298"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1405"/>
            <end position="1437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="10"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="156"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="466"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="477"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
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            <begin position="888"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1313"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00069785"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
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    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="EMBL" id="AHH42282.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017335518.1"/>
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    </evidence>
    <sequence length="1633" mass="186813" checksum="E19FCA02CF8D7937" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5UL66</accession>
    <name>W5UL66_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10 12">Alpha-actinin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Actn2</name>
        <name evidence="12" type="synonym">actn2b</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alpha-actinin family.</text>
    </comment>
    <dbReference type="EMBL" id="JT418868">
        <property type="protein sequence ID" value="AHH42879.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017332685.1">
        <property type="nucleotide sequence ID" value="XM_017477196.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UL66"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022215"/>
    <dbReference type="GeneID" id="108270464"/>
    <dbReference type="KEGG" id="ipu:108270464"/>
    <dbReference type="CTD" id="641575"/>
    <dbReference type="OrthoDB" id="10017054at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21214">
        <property type="entry name" value="CH_ACTN_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21216">
        <property type="entry name" value="CH_ACTN_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000004">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000001">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000004">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000005">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000005">
        <property type="entry name" value="Actinin alpha 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000018">
        <property type="entry name" value="Actinin, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000002">
        <property type="entry name" value="Actinin, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000003">
        <property type="entry name" value="Actinin, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014837">
        <property type="entry name" value="EF-hand_Ca_insen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08726">
        <property type="entry name" value="EFhand_Ca_insen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01184">
        <property type="entry name" value="efhand_Ca_insen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="38"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="151"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="753"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="789"/>
            <end position="824"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AHH42879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017332685.1"/>
        </source>
    </evidence>
    <sequence length="894" mass="103197" checksum="A32B84D62455B107" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5ULW8</accession>
    <name>W5ULW8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Alpha-soluble NSF attachment protein</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="10 11">N-ethylmaleimide-sensitive factor attachment protein, alpha b isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Napa</name>
        <name evidence="10 11" type="synonym">napab</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SNAP family.</text>
    </comment>
    <dbReference type="EMBL" id="JT418389">
        <property type="protein sequence ID" value="AHH42720.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017321812.1">
        <property type="nucleotide sequence ID" value="XM_017466323.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017321813.1">
        <property type="nucleotide sequence ID" value="XM_017466324.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ULW8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000509"/>
    <dbReference type="GeneID" id="108264617"/>
    <dbReference type="KEGG" id="ipu:108264617"/>
    <dbReference type="CTD" id="327082"/>
    <dbReference type="GeneTree" id="ENSGT00390000005826"/>
    <dbReference type="OMA" id="WSVKEYL"/>
    <dbReference type="OrthoDB" id="9984275at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070044">
        <property type="term" value="C:synaptobrevin 2-SNAP-25-syntaxin-1a complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043195">
        <property type="term" value="C:terminal bouton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005483">
        <property type="term" value="F:soluble NSF attachment protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010807">
        <property type="term" value="P:regulation of synaptic vesicle priming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035494">
        <property type="term" value="P:SNARE complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035249">
        <property type="term" value="P:synaptic transmission, glutamatergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15832">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000028">
        <property type="entry name" value="beta-soluble NSF attachment protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000744">
        <property type="entry name" value="NSF_attach"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768:SF23">
        <property type="entry name" value="ALPHA-SOLUBLE NSF ATTACHMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768">
        <property type="entry name" value="SOLUBLE NSF ATTACHMENT PROTEIN SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14938">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00448">
        <property type="entry name" value="NSFATTACHMNT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AHH42720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017321812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017321813.1"/>
        </source>
    </evidence>
    <sequence length="295" mass="33348" checksum="13603DDF26234208" modified="2014-04-16" version="1">MDNSTKEKEAIALMAEAEKKVRSSQSFFGSLFGGSSKMEEACDTYGRAANMFKMAKNWSAAGNAFCQAALLHLQMQSKHDAATNFIDAGNAFKKADPQEAINCLIRAIEIYTDMGRFTIAAKHHVTIAEIYETELVDIDKAIAHYEQAADYYKGEESTSSANKCLLKVAAYAAQLEQYPKAIEIYEQVGTQAMDSTLLKYSAKDYFFKAALCHFCVDMLNAKLALQKYEEMFPAFSDSRECKLVKKLLDAYEEQNVDMYTDSVKEYDIISRLDQWLTTILLRIKKTIQEDESDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PJY1</accession>
    <name>A0A2D0PJY1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Tenascin-X isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC108255457</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017306923.1">
        <property type="nucleotide sequence ID" value="XM_017451434.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PJY1"/>
    <dbReference type="GeneID" id="108255457"/>
    <dbReference type="KEGG" id="ipu:108255457"/>
    <dbReference type="OrthoDB" id="7357196at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050991">
        <property type="entry name" value="ECM_Regulatory_Proteins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708:SF11">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE ETA-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708">
        <property type="entry name" value="TENASCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="4">
        <location>
            <begin position="29"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="409"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="411"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="444"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="458"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017306923.1"/>
        </source>
    </evidence>
    <sequence length="468" mass="51980" checksum="30EA7991AA21B9B2" modified="2017-12-20" version="1">MQMICLFHAFVKKGNKEGQILRPSSLASPPKSVGVIIKARTETLLTLEWSKVNNFSYILRDSNGTEIPLTVSADGSVLRCTVSSLSPGTKYSFVLYAMLKGLKHSTFNFTAVTSPSNVASVSVKARSETALTFEWSKVNNNNAYIYILRQSNRSEMYTPMYWGESMARRTVSSLRPGTKYTFTLYTVFFGERSSGYNFSAATMPVNVARVNVTQRFTDQLEFSWEEVKSSNISYILRDSSKAETTIAALGKDSVIRHTVSALSPGTRYRFTLYTVFEGVRSRGLTFTSVTASLTVTGVHCEHLPGGTALVLVWDAPSGQWTGVEVQMKGRNPQYLNGTRLEFHDLHPALWYDVTLKLCSGDVKGAPVSIRCQTDPRGVIAGVILVLLFIIFLVCLGVVTHRRSVLKRKAQSDFNTNQASSQEAEEGAHNAPVLAKQQSSDGKKHCGQRMNKKKRVKPIQQNPYINPYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PKN5</accession>
    <name>A0A2D0PKN5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">EF-hand and coiled-coil domain-containing protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">efcc1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017305976.1">
        <property type="nucleotide sequence ID" value="XM_017450487.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PKN5"/>
    <dbReference type="GeneID" id="108254953"/>
    <dbReference type="KEGG" id="ipu:108254953"/>
    <dbReference type="CTD" id="79825"/>
    <dbReference type="OrthoDB" id="10054715at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031601">
        <property type="entry name" value="CCD48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15799">
        <property type="entry name" value="CCD48"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="44"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="148"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="661"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="181"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="160"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017305976.1"/>
        </source>
    </evidence>
    <sequence length="681" mass="77707" checksum="0186F7B0A8AFC1E2" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PNF9</accession>
    <name>A0A2D0PNF9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ADP-ribosylation factor-like protein 6-interacting protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">arl6ip4</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ARL6IP4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017307546.1">
        <property type="nucleotide sequence ID" value="XM_017452057.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017307547.1">
        <property type="nucleotide sequence ID" value="XM_017452058.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PNF9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030799"/>
    <dbReference type="GeneID" id="108255808"/>
    <dbReference type="KEGG" id="ipu:108255808"/>
    <dbReference type="CTD" id="51329"/>
    <dbReference type="OMA" id="WHKSARE"/>
    <dbReference type="OrthoDB" id="48562at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019532">
        <property type="entry name" value="Nucl_RNA-splicing_assoc_SR-25"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10500">
        <property type="entry name" value="SR-25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="164"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="17"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="33"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="76"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="95"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="114"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="232"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017307546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017307547.1"/>
        </source>
    </evidence>
    <sequence length="253" mass="28633" checksum="386C3CC830F03A80" modified="2017-12-20" version="1">MGRSRSRSRSPAISVSRHGKERRSSKKERHRSSSSSSSSSSASPSPHKKRAHGSSKSRSAECKSEKKSRKRKHRSSSSSSSSSSSSSSSDDEAKKRKHKKAKKQLKKIKEKERKKVKKEKKRLKKMKKLAARAEAAANKAPLMIPVPVPVQIEKTQSYIETWQSDDTTEHGPVMTDEQKASFSTKRPMTKEEYEARQSVIRRVVDPQTGRTRLVRGDGEILEEIVSREKHKDINKQATKRDGDAFQKRLGINR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PRJ2</accession>
    <name>A0A2D0PRJ2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="31" type="primary">grin1b</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30">SDA103</strain>
        </source>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="31">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="28">Receptor for glutamate that functions as a ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="28">Postsynaptic cell membrane</location>
            <topology evidence="28">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Postsynaptic density membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047005912.1">
        <property type="nucleotide sequence ID" value="XM_047149956.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PRJ2"/>
    <dbReference type="GeneID" id="108255876"/>
    <dbReference type="KEGG" id="ipu:108255876"/>
    <dbReference type="CTD" id="100005675"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017146">
        <property type="term" value="C:NMDA selective glutamate receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004972">
        <property type="term" value="F:NMDA glutamate receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070588">
        <property type="term" value="P:calcium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06379">
        <property type="entry name" value="PBP1_iGluR_NMDA_NR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13719">
        <property type="entry name" value="PBP2_iGluR_NMDA_Nr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000010">
        <property type="entry name" value="glutamate receptor ionotropic, NMDA 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000025">
        <property type="entry name" value="glutamate receptor ionotropic, NMDA 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000012">
        <property type="entry name" value="glutamate receptor ionotropic, NMDA 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000204">
        <property type="entry name" value="glutamate receptor ionotropic, NMDA 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000025">
        <property type="entry name" value="glutamate receptor ionotropic, NMDA 1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000087">
        <property type="entry name" value="glutamate receptor ionotropic, NMDA 1 isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049872">
        <property type="entry name" value="NMDA1-like_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049873">
        <property type="entry name" value="NMDA1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00177">
        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 28" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15 27" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="20 28" id="KW-0407">Ion channel</keyword>
    <keyword evidence="13 28" id="KW-0406">Ion transport</keyword>
    <keyword evidence="19 28" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 28" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18 28" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="16 28 31" id="KW-0675">Receptor</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 28" id="KW-0732">Signal</keyword>
    <keyword evidence="12 28" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 28" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 28" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 28" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="28">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039777223" description="Glutamate receptor" evidence="28">
        <location>
            <begin position="18"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="578"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="651"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="829"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="906"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="922"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="532"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="534"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="539"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="704"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="748"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="26">
        <location>
            <position position="680"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="26">
        <location>
            <position position="710"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="27">
        <location>
            <begin position="760"/>
            <end position="814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00034112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="RuleBase" id="RU367118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="RefSeq" id="XP_047005912.1"/>
        </source>
    </evidence>
    <sequence length="937" mass="105628" checksum="95113EA9B4904A65" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PT46</accession>
    <name>A0A2D0PT46_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="18 19 20">DNA topoisomerase 2-binding protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18 19 20" type="primary">topbp1</name>
    </gene>
    <organism evidence="17 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18 19 20" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18 19 20">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
            <location evidence="4">Spindle pole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the TOPBP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017308599.1">
        <property type="nucleotide sequence ID" value="XM_017453110.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017308600.1">
        <property type="nucleotide sequence ID" value="XM_017453111.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017308601.1">
        <property type="nucleotide sequence ID" value="XM_017453112.3"/>
    </dbReference>
    <dbReference type="GeneID" id="108256351"/>
    <dbReference type="KEGG" id="ipu:108256351"/>
    <dbReference type="CTD" id="11073"/>
    <dbReference type="OrthoDB" id="251770at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17737">
        <property type="entry name" value="BRCT_TopBP1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17731">
        <property type="entry name" value="BRCT_TopBP1_rpt2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17718">
        <property type="entry name" value="BRCT_TopBP1_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17749">
        <property type="entry name" value="BRCT_TopBP1_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18434">
        <property type="entry name" value="BRCT_TopBP1_rpt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17727">
        <property type="entry name" value="BRCT_TopBP1_rpt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17738">
        <property type="entry name" value="BRCT_TopBP1_rpt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17728">
        <property type="entry name" value="BRCT_TopBP1_rpt8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000018">
        <property type="entry name" value="DNA topoisomerase 2-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000028">
        <property type="entry name" value="DNA topoisomerase 2-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000010">
        <property type="entry name" value="DNA topoisomerase II binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000020">
        <property type="entry name" value="DNA topoisomerase II binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000021">
        <property type="entry name" value="DNA topoisomerase II binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000023">
        <property type="entry name" value="DNA topoisomerase II binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000029">
        <property type="entry name" value="DNA topoisomerase II binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059215">
        <property type="entry name" value="BRCT2_TopBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049542">
        <property type="entry name" value="TopBP1-like_BRCT0"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044737">
        <property type="entry name" value="TopBP1_BRCT_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049936">
        <property type="entry name" value="TopBP1_BRCT_8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13561">
        <property type="entry name" value="DNA REPLICATION REGULATOR DPB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13561:SF20">
        <property type="entry name" value="DNA TOPOISOMERASE 2-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12738">
        <property type="entry name" value="PTCB-BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21298">
        <property type="entry name" value="TopBP1_BRCT0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="165"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="258"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="417"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="598"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="691"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="970"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="1345"/>
            <end position="1423"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="1458"/>
            <end position="1555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="349"/>
            <end position="375"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="521"/>
            <end position="546"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="790"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1074"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1157"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1236"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1291"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1561"/>
            <end position="1605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="358"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="521"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1074"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1118"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1564"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017308599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017308600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017308601.1"/>
        </source>
    </evidence>
    <sequence length="1624" mass="179976" checksum="239B592AF620DC84" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PWJ7</accession>
    <name>A0A2D0PWJ7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">CD164 sialomucin-like 2 protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cd164l2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD164 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047006425.1">
        <property type="nucleotide sequence ID" value="XM_047150469.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PWJ7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029838"/>
    <dbReference type="GeneID" id="108256971"/>
    <dbReference type="CTD" id="388611"/>
    <dbReference type="OMA" id="VQGGCKQ"/>
    <dbReference type="OrthoDB" id="6160056at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007947">
        <property type="entry name" value="CD164_MGC24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11337:SF11">
        <property type="entry name" value="CD164 SIALOMUCIN-LIKE 2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11337">
        <property type="entry name" value="MUCIN/PORIMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05283">
        <property type="entry name" value="MGC-24"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_047006425.1"/>
        </source>
    </evidence>
    <sequence length="153" mass="16532" checksum="6F0AF82870DF4680" modified="2017-12-20" version="1">MRCVTVVLCCTVLLMQLIMTSSQTAEDSAISDCSQLDSCDRCTSGDPALNLTRCIWRSCDNDNNTRCISDTEDSGDCAVYNDTNSCPFRESFDDGGPDDSSSGSAPELSQASFDLSSFIGGIILVLSLQAGAFFALRFLKTKDSTYETIDQPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PWP7</accession>
    <name>A0A2D0PWP7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18 19" type="primary">LOC108257321</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18 19" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18 19">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017310464.1">
        <property type="nucleotide sequence ID" value="XM_017454975.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017310465.1">
        <property type="nucleotide sequence ID" value="XM_017454976.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PWP7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000017530"/>
    <dbReference type="GeneID" id="108257321"/>
    <dbReference type="KEGG" id="ipu:108257321"/>
    <dbReference type="OMA" id="TYWNLRK"/>
    <dbReference type="OrthoDB" id="1854502at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120015">
        <property type="term" value="F:sterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006699">
        <property type="term" value="P:bile acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13287">
        <property type="entry name" value="PH_ORP3_ORP6_ORP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000011">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000001">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3490:FF:000002">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041680">
        <property type="entry name" value="PH_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF146">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15409">
        <property type="entry name" value="PH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 14" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="11" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PH" evidence="16">
        <location>
            <begin position="57"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="461"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017310464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017310465.1"/>
        </source>
    </evidence>
    <sequence length="894" mass="101514" checksum="2B280D0890D658A0" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q039</accession>
    <name>A0A2D0Q039_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-binding protein RFX6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Regulatory factor X 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">rfx6</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017311664.1">
        <property type="nucleotide sequence ID" value="XM_017456175.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q039"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030923"/>
    <dbReference type="GeneID" id="108257977"/>
    <dbReference type="KEGG" id="ipu:108257977"/>
    <dbReference type="CTD" id="222546"/>
    <dbReference type="OMA" id="FAKNWEH"/>
    <dbReference type="OrthoDB" id="10056949at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031018">
        <property type="term" value="P:endocrine pancreas development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000211">
        <property type="entry name" value="Regulatory factor X, 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003150">
        <property type="entry name" value="DNA-bd_RFX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039779">
        <property type="entry name" value="RFX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057321">
        <property type="entry name" value="RFX1-4/6/8-like_BCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12619:SF28">
        <property type="entry name" value="DNA-BINDING PROTEIN RFX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12619">
        <property type="entry name" value="RFX TRANSCRIPTION FACTOR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25340">
        <property type="entry name" value="BCD_RFX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02257">
        <property type="entry name" value="RFX_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51526">
        <property type="entry name" value="RFX_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RFX-type winged-helix" evidence="11">
        <location>
            <begin position="109"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00077088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017311664.1"/>
        </source>
    </evidence>
    <sequence length="907" mass="100624" checksum="98651DDA3DA42BF2" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q1B7</accession>
    <name>A0A2D0Q1B7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">T cell activation RhoGTPase activating protein b isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tagapb</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017311476.2">
        <property type="nucleotide sequence ID" value="XM_017455987.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q1B7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032424"/>
    <dbReference type="GeneID" id="108257876"/>
    <dbReference type="KEGG" id="ipu:108257876"/>
    <dbReference type="CTD" id="447809"/>
    <dbReference type="OrthoDB" id="27389at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035023">
        <property type="term" value="P:regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04402">
        <property type="entry name" value="RhoGAP_ARHGAP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047886">
        <property type="entry name" value="ARHGAP20-like_RhoGAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047887">
        <property type="entry name" value="ARHGAP20_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23179">
        <property type="entry name" value="T-CELL ACTIVATION RHO GTPASE ACTIVATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23179:SF26">
        <property type="entry name" value="T-CELL ACTIVATION RHO GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22286">
        <property type="entry name" value="RHG20_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="272"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="467"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="727"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="735"/>
            <end position="745"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017311476.2"/>
        </source>
    </evidence>
    <sequence length="841" mass="93827" checksum="5C080119708BE8B9" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q238</accession>
    <name>A0A2D0Q238_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Homeobox protein OTX1 A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">otx2a</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017312368.1">
        <property type="nucleotide sequence ID" value="XM_017456879.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q238"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008433"/>
    <dbReference type="GeneID" id="108258325"/>
    <dbReference type="KEGG" id="ipu:108258325"/>
    <dbReference type="CTD" id="30462"/>
    <dbReference type="OMA" id="YFSGVEC"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003406">
        <property type="term" value="P:retinal pigment epithelium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035295">
        <property type="term" value="P:tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000142">
        <property type="entry name" value="homeobox protein OTX2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003025">
        <property type="entry name" value="Otx_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013851">
        <property type="entry name" value="Otx_TF_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45793">
        <property type="entry name" value="HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45793:SF28">
        <property type="entry name" value="HOMEOBOX PROTEIN OTX1 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03529">
        <property type="entry name" value="TF_Otx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01255">
        <property type="entry name" value="OTXHOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="38"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="93"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="254"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="95"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="139"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
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            <begin position="181"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="275"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017312368.1"/>
        </source>
    </evidence>
    <sequence length="354" mass="37870" checksum="9187330618076E2C" modified="2017-12-20" version="1">MMSYLKQAPYAVNGLGLGGAPMELLHPSVGYPANPRKQRRERTTFTRTQLDILESLFAKTRYPDIFMREEVALKINLPESRVQVWFKNRRAKCRQQQQSSGSSNSGNSAKIRPAKKKASPSRESPGSESSGQFSPPAVSSTSSSSSSSSSSSSNSNLGGLGMTSSSASGATPVSSIWSPAPISPLPAPPSVPDPAPPTSASCMQRAVSGSGGPAGVASYPVSYNQTPSYAQSYPSTASSSYFGSMDCSSYLAPMHSHHPHHPQLSSMAGSGVSGHHTHPHHPHHLGQSNGHHHPHHHPHAHTHQGYGGAPLPFTSTDCVDYKDYKDQAASPWKLNFSADCLDYKDQAAWRFQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q602</accession>
    <name>A0A2D0Q602_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Galectin-3-binding protein A isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC108258750</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017313131.1">
        <property type="nucleotide sequence ID" value="XM_017457642.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q602"/>
    <dbReference type="GeneID" id="108258750"/>
    <dbReference type="OrthoDB" id="25028at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000001">
        <property type="entry name" value="Lysyl oxidase 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051481">
        <property type="entry name" value="BTB-POZ/Galectin-3-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24410:SF16">
        <property type="entry name" value="GALECTIN-3-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24410">
        <property type="entry name" value="HL07962P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013107491" evidence="9">
        <location>
            <begin position="22"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="11">
        <location>
            <begin position="38"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="10">
        <location>
            <begin position="170"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="63"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="76"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017313131.1"/>
        </source>
    </evidence>
    <sequence length="572" mass="65592" checksum="B896F6DD7E59F50C" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q7J8</accession>
    <name>A0A2D0Q7J8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BTB/POZ domain-containing protein KCTD19 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC108259361</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017314272.1">
        <property type="nucleotide sequence ID" value="XM_017458783.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q7J8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000025937"/>
    <dbReference type="GeneID" id="108259361"/>
    <dbReference type="KEGG" id="ipu:108259361"/>
    <dbReference type="OMA" id="EEMFYAR"/>
    <dbReference type="OrthoDB" id="10003873at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499:SF20">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN KCTD19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499">
        <property type="entry name" value="POTASSIUM CHANNEL TETRAMERIZATION DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Potassium channel tetramerisation-type BTB" evidence="2">
        <location>
            <begin position="11"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="475"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="494"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017314272.1"/>
        </source>
    </evidence>
    <sequence length="873" mass="98451" checksum="662B48AAE1338C28" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QBM9</accession>
    <name>A0A2D0QBM9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Glucose-6-phosphate exchanger SLC37A2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Solute carrier family 37 member 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">slc37a2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>D-glucose 6-phosphate(in) + phosphate(out) = D-glucose 6-phosphate(out) + phosphate(in)</text>
            <dbReference type="Rhea" id="RHEA:71535"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017316098.1">
        <property type="nucleotide sequence ID" value="XM_017460609.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QBM9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033814"/>
    <dbReference type="GeneID" id="108260365"/>
    <dbReference type="KEGG" id="ipu:108260365"/>
    <dbReference type="CTD" id="219855"/>
    <dbReference type="OMA" id="AMPYLID"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061513">
        <property type="term" value="F:glucose 6-phosphate:phosphate antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17344">
        <property type="entry name" value="MFS_SLC37A1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000050">
        <property type="entry name" value="glucose-6-phosphate exchanger SLC37A2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000028">
        <property type="entry name" value="Sugar phosphate exchanger 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044740">
        <property type="entry name" value="SLC37A1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000849">
        <property type="entry name" value="Sugar_P_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43184:SF9">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE EXCHANGER SLC37A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43184">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER 16, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002808">
        <property type="entry name" value="Hexose_phosphate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="142"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="332"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="356"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="387"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="422"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="456"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="15">
        <location>
            <begin position="28"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017316098.1"/>
        </source>
    </evidence>
    <sequence length="495" mass="54139" checksum="D95BA2C9664CC0F5" modified="2017-12-20" version="1">MKSSLAPGIRVITSFSRDSWYRGFILFLTFLFYTAYHLSRKPISIVKSQLHRNCSYVIRPTDLNVTDNSTWCDWAPFDRDNYQNLFGWLDNCFLVAYAIGMFFSGIFGERLPLRYYLTTGMLLSGLFTALFGLGFYWNIHSLWYYCLVQVLNGLVQTTGWPAVVACVGNWFGKGKRGFIMGVWNSHTSVGNILGSLIAGAFVSTAWGMSFIVPGIIIATTGVICFFFLVEHPEDVSCSPPQHHESSEREPLLRTSLNSEEIFNNHTTAVVEAGEEEAEAISFVGALRIPGVVEFSLCLLFAKLVSYTFLYWLPLYIANVAHFDPKAAGDMSTLFDVGGILGGILAGLVSDYTGGRASTCCVMLIVAAPMLFLYNLVGQRGVPTTVGMLLLCGALVNGPYALITTAVSADLGTHECLRGNARALSTVTAIIDGTGSIGAAVGPLLAGLISPTGWNNVFYMLITADVLACLLLSRLVFKEIRGWCGYSTRPRGFKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QGI4</accession>
    <name>A0A2D0QGI4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aprataxin</fullName>
            <ecNumber evidence="4">3.6.1.71</ecNumber>
            <ecNumber evidence="3">3.6.1.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Forkhead-associated domain histidine triad-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">aptx</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non-ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined. Also able to hydrolyze adenosine 5'-monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity. Likewise, catalyzes the release of 3'-linked guanosine (DNAppG) and inosine (DNAppI) from DNA, but has higher specific activity with 5'-linked adenosine (AppDNA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 3'-end 2'-deoxyribonucleotide-3'-diphospho-5'-guanosine-DNA + H2O = a 3'-end 2'-deoxyribonucleotide 3'-phosphate-DNA + GMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:52140"/>
            <dbReference type="Rhea" id="RHEA-COMP:13186"/>
            <dbReference type="Rhea" id="RHEA-COMP:13187"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:136419"/>
            <dbReference type="ChEBI" id="CHEBI:136420"/>
            <dbReference type="EC" id="3.6.1.72"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 5'-end adenosine-5'-diphospho-5'-2'-deoxyribonucleoside-DNA + H2O = a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:52128"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:13181"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:136413"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.6.1.71"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 5'-end adenosine-5'-diphospho-5'-ribonucleoside-2'-deoxyribonucleotide-DNA + H2O = a 5'-end 5'-phospho-ribonucleoside-2'-deoxyribonucleotide-DNA + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:52132"/>
            <dbReference type="Rhea" id="RHEA-COMP:13182"/>
            <dbReference type="Rhea" id="RHEA-COMP:13183"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136414"/>
            <dbReference type="ChEBI" id="CHEBI:136415"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.6.1.71"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.6.1.71" evidence="4"/>
    <dbReference type="EC" id="3.6.1.72" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017316515.1">
        <property type="nucleotide sequence ID" value="XM_017461026.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QGI4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000025931"/>
    <dbReference type="GeneID" id="108260610"/>
    <dbReference type="KEGG" id="ipu:108260610"/>
    <dbReference type="CTD" id="54840"/>
    <dbReference type="OMA" id="QFRTGYH"/>
    <dbReference type="OrthoDB" id="3512845at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033699">
        <property type="term" value="F:DNA 5'-adenosine monophosphate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120108">
        <property type="term" value="F:DNA-3'-diphospho-5'-guanosine diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990165">
        <property type="term" value="F:single-strand break-containing DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000012">
        <property type="term" value="P:single strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01278">
        <property type="entry name" value="aprataxin_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000004">
        <property type="entry name" value="aprataxin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041388">
        <property type="entry name" value="FHA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032566">
        <property type="entry name" value="Znf-C2HE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12486:SF4">
        <property type="entry name" value="APRATAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12486">
        <property type="entry name" value="APRATAXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11969">
        <property type="entry name" value="DcpS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17913">
        <property type="entry name" value="FHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16278">
        <property type="entry name" value="zf-C2HE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HIT" evidence="21">
        <location>
            <begin position="165"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="104"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="19">
        <location>
            <begin position="255"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="121"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="151"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00045142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017316515.1"/>
        </source>
    </evidence>
    <sequence length="339" mass="38698" checksum="DE052C230E914CE7" modified="2017-12-20" version="1">MPTCWLVRADESHKPIQLLHYHTHTLGRGPETQIKDQKCSRQQVELRADCNRGFVTVKQLGSNPTSLDDVVVGKGNQVTFKPGQKLYMVNRLYPYTLQFREESTSAGASGEKSKAMKRPHQRDAHEERDQDKEKSCRVSAPSCSNSAARPDQTHAPEKEPAAHWSQGLKVSMQDPSMQVYKDEKVVVIKDKYPKARFHWLVLPWDSISSLKALRPEHVELLKHMERIGARMAQQCPGSDTLRFRLGYHAIPSMSHVHLHVISQDFDSPCLKNKKHWNSFTTDYFIESQDVIAMLERDGRVSVKDGTSELLKLPLRCHVCHQEQTTIPKLKEHLRGHASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QHN2</accession>
    <name>A0A2D0QHN2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Sodium/potassium/calcium exchanger 2-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">slc24a2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Ca(2+)(out) + K(+)(out) + 4 Na(+)(in) = Ca(2+)(in) + K(+)(in) + 4 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:69967"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC24A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017316990.1">
        <property type="nucleotide sequence ID" value="XM_017461501.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QHN2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033380"/>
    <dbReference type="GeneID" id="108260852"/>
    <dbReference type="KEGG" id="ipu:108260852"/>
    <dbReference type="CTD" id="25769"/>
    <dbReference type="OrthoDB" id="2127281at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008273">
        <property type="term" value="F:calcium, potassium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060292">
        <property type="term" value="P:long-term synaptic depression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060291">
        <property type="term" value="P:long-term synaptic potentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000002">
        <property type="entry name" value="Sodium/potassium/calcium exchanger 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000004">
        <property type="entry name" value="Sodium/potassium/calcium exchanger 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004481">
        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00367">
        <property type="entry name" value="calcium/sodium antiporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="SODIUM/POTASSIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846:SF41">
        <property type="entry name" value="SODIUM_POTASSIUM_CALCIUM EXCHANGER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0769">Symport</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="389"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="418"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="487"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="520"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="550"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="16">
        <location>
            <begin position="99"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="16">
        <location>
            <begin position="419"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017316990.1"/>
        </source>
    </evidence>
    <sequence length="580" mass="65109" checksum="0CA9D5EACE3BB17F" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QIB7</accession>
    <name>A0A2D0QIB7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Neuronal acetylcholine receptor subunit alpha-7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">Nicotinic acetylcholine receptor subunit alpha-7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="primary">chrna8</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="29">SDA103</strain>
        </source>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="30">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-arginine(in) = L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:32143"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NH4(+)(in) = NH4(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28747"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>choline(out) = choline(in)</text>
            <dbReference type="Rhea" id="RHEA:32751"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>guanidine(out) = guanidine(in)</text>
            <dbReference type="Rhea" id="RHEA:73883"/>
            <dbReference type="ChEBI" id="CHEBI:30087"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="23">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub-subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017317265.1">
        <property type="nucleotide sequence ID" value="XM_017461776.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QIB7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000002398"/>
    <dbReference type="GeneID" id="108260988"/>
    <dbReference type="KEGG" id="ipu:108260988"/>
    <dbReference type="CTD" id="100537531"/>
    <dbReference type="OrthoDB" id="5975154at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19051">
        <property type="entry name" value="LGIC_TM_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000007">
        <property type="entry name" value="Neuronal acetylcholine receptor subunit alpha-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000011">
        <property type="entry name" value="neuronal acetylcholine receptor subunit alpha-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000009">
        <property type="entry name" value="Neuronal acetylcholine receptor subunit alpha-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15 26" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 26" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="11 30" id="KW-0675">Receptor</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 26" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
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    <keyword evidence="6 26" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 26" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
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        <location>
            <begin position="27"/>
            <end position="497"/>
        </location>
    </feature>
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        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="295"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="465"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="27">
        <location>
            <begin position="30"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="28">
        <location>
            <begin position="240"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00034423"/>
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            <dbReference type="ARBA" id="ARBA00036239"/>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00081631"/>
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        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="RefSeq" id="XP_017317265.1"/>
        </source>
    </evidence>
    <sequence length="497" mass="55990" checksum="4DB991E7EC41B261" modified="2017-12-20" version="1">MPRFTGPLYVVLFMWSSFCIKDTIQGEHQRRLYKELLESYNRLERPVANDSAPLVVELGLTLLQIIDVDEKNQVLITNAWLQMDWTDVYLSWNPENYPGVNNLRFPSSQIWTPDILLYNSADERFDATFHTNVLVNSSGACQYIPPGILKSTCYIDVRWFPFDVQKCDLKFGSWTHNGWLLDLQMREVDISTYIPNGEWDLVGVPGKRNELYYECCKEPYPDVTYTVTIRRRTLYYGLNLLIPCVLISGLALLVFLLPADSGEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIAQYFASTMMIVGLSVVVTVLVLQFHHHDPHGGKMPKWVRVILLHWCAWFLRMKKPGDERKSPYSYSSTSSLQMGSVPGQMGTNGHTNLYFGFHSPDSTCCPNSSDSGVSCGVSCGGGHREAAPALSGEQRELQRLLVEVSYMAQRFRAQDEAEAVRGEWKFAAAVVDRLCLVAFSLFSIVCTFAILMSAPNFIEAVSKDFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QUZ0</accession>
    <name>A0A2D0QUZ0_ICTPU</name>
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        <submittedName>
            <fullName evidence="6">Ran-binding protein 10 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ranbp10</name>
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    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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            <taxon>Ictaluridae</taxon>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
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    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RANBP9/10 family.</text>
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        <property type="nucleotide sequence ID" value="XM_017465816.3"/>
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        <property type="component" value="Chromosome 4"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="LISH"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="68"/>
            <end position="255"/>
        </location>
    </feature>
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            <begin position="324"/>
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        </location>
    </feature>
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        <location>
            <begin position="388"/>
            <end position="484"/>
        </location>
    </feature>
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        <location>
            <begin position="388"/>
            <end position="401"/>
        </location>
    </feature>
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        <location>
            <begin position="416"/>
            <end position="431"/>
        </location>
    </feature>
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        <location>
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            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="466"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017321305.1"/>
        </source>
    </evidence>
    <sequence length="661" mass="72359" checksum="EDE6CED68D8E1D21" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QWQ0</accession>
    <name>A0A2D0QWQ0_ICTPU</name>
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        <submittedName>
            <fullName evidence="3">VPS9 domain-containing protein 1</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">vps9d1</name>
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    <organism evidence="2 3">
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    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047010832.1">
        <property type="nucleotide sequence ID" value="XM_047154876.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QWQ0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000023422"/>
    <dbReference type="GeneID" id="124627950"/>
    <dbReference type="KEGG" id="ipu:124627950"/>
    <dbReference type="CTD" id="9605"/>
    <dbReference type="OMA" id="CLKPAMP"/>
    <dbReference type="OrthoDB" id="10264848at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="RAB GDP/GTP EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101:SF98">
        <property type="entry name" value="VPS9 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="382"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_047010832.1"/>
        </source>
    </evidence>
    <sequence length="692" mass="77488" checksum="731CD4653BD42AFB" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QZ90</accession>
    <name>A0A2D0QZ90_ICTPU</name>
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        <submittedName>
            <fullName evidence="8">Piercer of microtubule wall 1 protein</fullName>
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        <name evidence="8" type="primary">pierce1</name>
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    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PIERCE1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017323639.1">
        <property type="nucleotide sequence ID" value="XM_017468150.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QZ90"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013320"/>
    <dbReference type="GeneID" id="108265611"/>
    <dbReference type="KEGG" id="ipu:108265611"/>
    <dbReference type="CTD" id="138162"/>
    <dbReference type="OMA" id="MFRNNTF"/>
    <dbReference type="OrthoDB" id="546383at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005879">
        <property type="term" value="C:axonemal microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060971">
        <property type="term" value="P:embryonic heart tube left/right pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026507">
        <property type="entry name" value="PIRC1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20899">
        <property type="entry name" value="PIERCE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20899:SF1">
        <property type="entry name" value="PIERCER OF MICROTUBULE WALL 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14892">
        <property type="entry name" value="PIRC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017323639.1"/>
        </source>
    </evidence>
    <sequence length="141" mass="16107" checksum="422498960CF168F6" modified="2017-12-20" version="1">MDAECLDAGINTEQQQSDKNTDLKTSDVYRVNENLPKRFNNPACFKGYSMKMAHPLYQTTNQIYGSKKPTVHEMPTTFHGSWHKFSDLLLKSGMFRDTGFNTSLEKSQITGPNTINMRDDRITFHRLYHPGGNEQAETNVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QZS8</accession>
    <name>A0A2D0QZS8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-alpha-acetyltransferase 35, NatC auxiliary subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Protein MAK10 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">naa35</name>
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    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAK10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017323030.1">
        <property type="nucleotide sequence ID" value="XM_017467541.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QZS8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013478"/>
    <dbReference type="GeneID" id="108265334"/>
    <dbReference type="KEGG" id="ipu:108265334"/>
    <dbReference type="CTD" id="60560"/>
    <dbReference type="OMA" id="QMEWIVQ"/>
    <dbReference type="OrthoDB" id="269405at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031417">
        <property type="term" value="C:NatC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057983">
        <property type="entry name" value="NAA35-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007244">
        <property type="entry name" value="Naa35_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057982">
        <property type="entry name" value="TPR_NAA35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373">
        <property type="entry name" value="GLUCOSE REPRESSIBLE PROTEIN MAK10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373:SF0">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 35, NATC AUXILIARY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04112">
        <property type="entry name" value="Mak10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25789">
        <property type="entry name" value="TPR_NAA35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAA35-like N-terminal" evidence="7">
        <location>
            <begin position="45"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="NAA35-like N-terminal" evidence="7">
        <location>
            <begin position="143"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="NAA35-like TPR repeats" evidence="8">
        <location>
            <begin position="337"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="547"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="558"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017323030.1"/>
        </source>
    </evidence>
    <sequence length="725" mass="83297" checksum="85703E32AAF87986" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R420</accession>
    <name>A0A2D0R420_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphodiesterase</fullName>
            <ecNumber evidence="11">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15 16 17" type="primary">si:dkey-219c10.4</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15 16 17" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15 16 17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Specifically hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. Highly specific: compared to other members of the cyclic nucleotide phosphodiesterase family, has the highest affinity and selectivity for cGMP. Specifically regulates natriuretic-peptide-dependent cGMP signaling in heart, acting as a regulator of cardiac hypertrophy in myocytes and muscle. Does not regulate nitric oxide-dependent cGMP in heart. Additional experiments are required to confirm whether its ability to hydrolyze natriuretic-peptide-dependent cGMP is specific to heart or is a general feature of the protein. In brain, involved in cognitive function, such as learning and long-term memory.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3',5'-cyclic GMP + H2O = GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16957"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="EC" id="3.1.4.35"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="11">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the cyclic nucleotide phosphodiesterase family. PDE9 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_017324796.1">
        <property type="nucleotide sequence ID" value="XM_017469307.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047012130.1">
        <property type="nucleotide sequence ID" value="XM_047156174.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053536874.1">
        <property type="nucleotide sequence ID" value="XM_053680899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R420"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000001720"/>
    <dbReference type="GeneID" id="108266238"/>
    <dbReference type="KEGG" id="ipu:108266238"/>
    <dbReference type="GeneTree" id="ENSGT00940000165301"/>
    <dbReference type="OMA" id="AYQINAQ"/>
    <dbReference type="OrthoDB" id="546632at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047555">
        <property type="term" value="F:3',5'-cyclic-GMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000006">
        <property type="entry name" value="Phosphodiesterase 9A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0140">cGMP</keyword>
    <keyword evidence="4 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="13">
        <location>
            <begin position="168"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="497"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="497"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="519"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="251"/>
            <end position="255"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="452"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017324796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_047012130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_053536874.1"/>
        </source>
    </evidence>
    <sequence length="534" mass="62136" checksum="92367455795A4C94" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R4N5</accession>
    <name>A0A2D0R4N5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ornithine carbamoyltransferase</fullName>
            <ecNumber evidence="5">2.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">otc</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Nitrogen metabolism; urea cycle; L-citrulline from L-ornithine and carbamoyl phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.3" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017325011.1">
        <property type="nucleotide sequence ID" value="XM_017469522.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R4N5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000016338"/>
    <dbReference type="GeneID" id="108266357"/>
    <dbReference type="KEGG" id="ipu:108266357"/>
    <dbReference type="CTD" id="5009"/>
    <dbReference type="OMA" id="DGNNVCN"/>
    <dbReference type="OrthoDB" id="10252326at2759"/>
    <dbReference type="UniPathway" id="UPA00158">
        <property type="reaction ID" value="UER00271"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019240">
        <property type="term" value="P:citrulline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000050">
        <property type="term" value="P:urea cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000009">
        <property type="entry name" value="Ornithine carbamoyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000010">
        <property type="entry name" value="Ornithine carbamoyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002292">
        <property type="entry name" value="Orn/put_carbamltrans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00658">
        <property type="entry name" value="orni_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001986">
        <property type="entry name" value="PRK00779.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753">
        <property type="entry name" value="ORNITHINE CARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753:SF3">
        <property type="entry name" value="ORNITHINE TRANSCARBAMYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00102">
        <property type="entry name" value="OTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0835">Urea cycle</keyword>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase carbamoyl-P binding" evidence="14">
        <location>
            <begin position="63"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase Asp/Orn-binding" evidence="13">
        <location>
            <begin position="210"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017325011.1"/>
        </source>
    </evidence>
    <sequence length="377" mass="42221" checksum="4330A65169812D72" modified="2017-12-20" version="1">MKAQAGFEKSLGFILVCAVTRLLISMLALRNTVLHSLKAVKSDQARSFRLGRTSLGCVNLKGRSFLTLKDFNPDEIKHILWVSADLKHRIKHEGQYLPLLQGKSIAMIFEKRSTRTRMSTETGFALLGGHPCFLTPQDIHLGVNESTKDTARVLSSFTDIVLARVYSHSTLEQLDKEASIPIINGLSDLYHPIQILADLLTLQEHYGSLKGLTMSWIGDGNNVLHSFMMSSAKLGINLRIATPKGYAPDANVTQEALRLSKEFGTELHLFSEPLEAAQGSNVLVTDTWISMGQEDEKMKRLKDFHGYKITMKTGSVAAQDWTFLHCLPRKSEEVDDEVFYSPRSLVFPEAENRKWTIMGLIACILTDYSPQIPKLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R5G3</accession>
    <name>A0A2D0R5G3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inhibin beta B chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Activin beta-B chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">inhbb</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017325809.1">
        <property type="nucleotide sequence ID" value="XM_017470320.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R5G3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035270"/>
    <dbReference type="GeneID" id="108266679"/>
    <dbReference type="KEGG" id="ipu:108266679"/>
    <dbReference type="CTD" id="3625"/>
    <dbReference type="OMA" id="SERMNID"/>
    <dbReference type="OrthoDB" id="6516235at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19405">
        <property type="entry name" value="TGF_beta_INHBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000005">
        <property type="entry name" value="Inhibin beta A chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000381">
        <property type="entry name" value="INHBB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF29">
        <property type="entry name" value="INHIBIN BETA B CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00671">
        <property type="entry name" value="INHIBINBB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 13" id="KW-0339">Growth factor</keyword>
    <keyword evidence="7" id="KW-0372">Hormone</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012609905" description="Inhibin beta B chain" evidence="14">
        <location>
            <begin position="21"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="15">
        <location>
            <begin position="264"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017325809.1"/>
        </source>
    </evidence>
    <sequence length="383" mass="42801" checksum="FACDCBFB74EF9FEE" modified="2017-12-20" version="1">MKVIMRVMCCLMACVLSVRGAGSDTHALPRDSCASCGLGRPDVPDAMDTDFLEAVKRHILSRLQMRQRPNITHPIPKAAMVTALRKLHAGKVREDGRFEIPSFDGQAAYSNEAQEETSEIISFAESDDLTSVKSSLYFVISNEGNQNLYVLQANLWLYIKLLPGGLEKGVRRKVTVRVHYQEPGGQQKWVEKRLELKRSGWHTFPLSEAVRVALEKDERRQDLDVHCESCEVANVAPVLVDVGDPSHRPFLVVRARQAEGKHRIHKRGLECDGNHGGLCCRQQFYIDFRLIGWNDWIIAPAGYFGNYCDGSCPAYMAGVPGSASSFHTAVVNQYRMRGISPGSVNSCCIPTKLSTMSMLYFDDEYNIVKRDVPNMIVEECGCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R804</accession>
    <name>A0A2D0R804_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Zinc finger protein 585A isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC108266882</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017326191.2">
        <property type="nucleotide sequence ID" value="XM_017470702.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R804"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021830"/>
    <dbReference type="GeneID" id="108266882"/>
    <dbReference type="OrthoDB" id="9439903at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002682">
        <property type="term" value="P:regulation of immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19193">
        <property type="entry name" value="PR-SET_PRDM7_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001155">
        <property type="entry name" value="Zinc finger 30C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RBS5</accession>
    <name>A0A2D0RBS5_ICTPU</name>
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        <name evidence="12" type="primary">epoa</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
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    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
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        <text evidence="9">Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.</text>
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        <subcellularLocation>
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        <text evidence="2 9">Belongs to the EPO/TPO family.</text>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:erythrocyte maturation"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 9" id="KW-0265">Erythrocyte maturation</keyword>
    <keyword evidence="5 9" id="KW-0372">Hormone</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="42"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="64"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR001951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001951-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017327325.1"/>
        </source>
    </evidence>
    <sequence length="193" mass="21573" checksum="B1790E0904F002BF" modified="2017-12-20" version="1">MDVTGHKERSKTIKRSAGLLALLLIILEWIRPGLPSPLRPICDLRVLNHFIKEARDAEAALRFCTEECSIAVPLTVPLTRVDFDVWEKQNTEEQAQEVQTGLWLLNMAISSLGASITNSALHSHIEASIRNIASLKQVLRSLSIQDYVPTSGGTEDTWKVSSASELFQVHINFLRGKVRLLLSNAPICQQHIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RBZ3</accession>
    <name>A0A2D0RBZ3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">poln</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA polymerase type-A family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017328059.1">
        <property type="nucleotide sequence ID" value="XM_017472570.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RBZ3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000010940"/>
    <dbReference type="GeneID" id="108267960"/>
    <dbReference type="KEGG" id="ipu:108267960"/>
    <dbReference type="CTD" id="353497"/>
    <dbReference type="OMA" id="LAMESHK"/>
    <dbReference type="OrthoDB" id="275278at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08638">
        <property type="entry name" value="DNA_pol_A_theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000002">
        <property type="entry name" value="DNA polymerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1060.10:FF:000001">
        <property type="entry name" value="DNA polymerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.10">
        <property type="entry name" value="Taq DNA Polymerase, Chain T, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001098">
        <property type="entry name" value="DNA-dir_DNA_pol_A_palm_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040940">
        <property type="entry name" value="DNA_pol_P_Exo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002298">
        <property type="entry name" value="DNA_polymerase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10133">
        <property type="entry name" value="DNA POLYMERASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10133:SF27">
        <property type="entry name" value="DNA POLYMERASE NU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00476">
        <property type="entry name" value="DNA_pol_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18049">
        <property type="entry name" value="DNA_pol_P_Exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00868">
        <property type="entry name" value="DNAPOLI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00482">
        <property type="entry name" value="POLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family A palm" evidence="12">
        <location>
            <begin position="931"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="87"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="401"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="107"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="409"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017328059.1"/>
        </source>
    </evidence>
    <sequence length="1192" mass="134477" checksum="F4E3DE5CED6CB183" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RDQ5</accession>
    <name>A0A2D0RDQ5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">HIG1 domain family member 1A, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">higd1a</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017328674.1">
        <property type="nucleotide sequence ID" value="XM_017473185.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RDQ5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008886"/>
    <dbReference type="GeneID" id="108268302"/>
    <dbReference type="KEGG" id="ipu:108268302"/>
    <dbReference type="CTD" id="25994"/>
    <dbReference type="OMA" id="SRGNTQM"/>
    <dbReference type="OrthoDB" id="10003563at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097250">
        <property type="term" value="P:mitochondrial respirasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007667">
        <property type="entry name" value="Hypoxia_induced_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050355">
        <property type="entry name" value="RCF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12297:SF3">
        <property type="entry name" value="HIG1 DOMAIN FAMILY MEMBER 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12297">
        <property type="entry name" value="HYPOXIA-INDUCBILE GENE 1 HIG1 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04588">
        <property type="entry name" value="HIG_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51503">
        <property type="entry name" value="HIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="22"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="HIG1" evidence="7">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017328674.1"/>
        </source>
    </evidence>
    <sequence length="95" mass="10882" checksum="C2AC17E4CE67FAE1" modified="2017-12-20" version="1">MSFMSFDEHEESKFLRKAKENPFVPVGMAGFFAIVAHRLYKMKYRGSMKMSVHLIHMRVAAQGFVVGAMTLGVVYSMYKDYVLKPAEAQRALENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RE32</accession>
    <name>A0A2D0RE32_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transmembrane protein 144b isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tmem144b</name>
        <name evidence="9" type="synonym">tm144</name>
        <name evidence="9" type="synonym">tmem144</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM144 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017328802.1">
        <property type="nucleotide sequence ID" value="XM_017473313.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RE32"/>
    <dbReference type="GeneID" id="100528843"/>
    <dbReference type="CTD" id="449810"/>
    <dbReference type="OrthoDB" id="426527at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015144">
        <property type="term" value="F:carbohydrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010651">
        <property type="entry name" value="Sugar_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012435">
        <property type="entry name" value="TMEM144"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16119">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 144"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16119:SF17">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 144"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07857">
        <property type="entry name" value="TMEM144"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013016968" evidence="7">
        <location>
            <begin position="26"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="233"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="369"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017328802.1"/>
        </source>
    </evidence>
    <sequence length="390" mass="41644" checksum="376E2C2BCEC23D77" modified="2017-12-20" version="1">MNSDGAVRSLGAVLFLVIFLSLMSGEVGDPQDEGDAGDAAGGVSGSSVHMEKVYGFVSCVVAVLLYGTIYVPVKKVETGDGMFYQWISCAAIWVVALIGDILLRSSKAHPAAMLGGMIWATGNICLVPVVKCVGLGLGMLLWGASGLLIGWASSLFGLFGLEPEVVSRPLLNYCGAALCLLSTLLFFFVKIDAQKPSCSEATPILIQERSFSDGLRSTDSWVDTISPKSRRPLGCIIAILSGLLFGSSFVPIIYIKHHAFSNNSMFTGSSQNDLDYCFAQSSGIFLMSMLYFVIYCAVMKNRPRVYPRAVLPGVLSGVMWGIATYCWLLANRRLGPVVTFPIVTAGYGLVAALWGSVVFKEVRGLGNCLLFAVACCMVLTGSILTVLSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0REI0</accession>
    <name>A0A2D0REI0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acyl-protein thioesterase 1</fullName>
            <ecNumber evidence="7">3.1.2.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Lysophospholipase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Lysophospholipase I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Palmitoyl-protein hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">lypla1</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">SDA103</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="27">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Acts as an acyl-protein thioesterase. Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Acts as a palmitoyl thioesterase that catalyzes depalmitoylation of proteins, such as ADRB2, KCNMA1 and SQSTM1. Acts as a negative regulator of autophagy by mediating palmitoylation of SQSTM1, decreasing affinity between SQSTM1 and ATG8 proteins and recruitment of ubiquitinated cargo proteins to autophagosomes. Acts as a lysophospholipase and hydrolyzes lysophosphatidylcholine (lyso-PC). Also hydrolyzes lysophosphatidylethanolamine (lyso-PE), lysophosphatidylinositol (lyso-PI) and lysophosphatidylserine (lyso-PS). Has much higher thioesterase activity than lysophospholipase activity. Contributes to the production of lysophosphatidic acid (LPA) during blood coagulation by recognizing and cleaving plasma phospholipids to generate lysophospholipids which in turn act as substrates for ENPP2 to produce LPA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40435"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:40436"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>S-hexadecanoyl-L-cysteinyl-[protein] + H2O = L-cysteinyl-[protein] + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="3.1.2.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>a 1-(9Z-octadecenoyl)-2-acyl-sn-glycero-3-phosphocholine + H2O = a 2-acyl-sn-glycero-3-phosphocholine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:57875"/>
            <dbReference type="ChEBI" id="CHEBI:78421"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:41721"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="7"/>
    <dbReference type="RefSeq" id="XP_017328295.1">
        <property type="nucleotide sequence ID" value="XM_017472806.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0REI0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000023977"/>
    <dbReference type="GeneID" id="108268102"/>
    <dbReference type="KEGG" id="ipu:108268102"/>
    <dbReference type="CTD" id="10434"/>
    <dbReference type="OMA" id="LMFRTYN"/>
    <dbReference type="OrthoDB" id="2418081at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000010">
        <property type="entry name" value="Acyl-protein thioesterase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050565">
        <property type="entry name" value="LYPA1-2/EST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655:SF22">
        <property type="entry name" value="ACYL-PROTEIN THIOESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phospholipase/carboxylesterase/thioesterase" evidence="25">
        <location>
            <begin position="11"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00014923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00042324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00045255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_017328295.1"/>
        </source>
    </evidence>
    <sequence length="229" mass="24526" checksum="07DC67DC3638BD7F" modified="2017-12-20" version="1">MCGNNMSAPLPAIVPAARKATAAVIFLHGLGDTGHGWADAMAAIRTPYVKYICPHAPVMPVTLNMNMAMPSWFDIIGLGPDAVEDETGIKKAAESINALIDQEVKNGIPSHRIVLGGFSQGGALSLYTALKTHQKLAGVVALSCWLPLRNSLSKSVIGTNKGIPVLQCHGEADPLVPLIFGCLTVEKLKTMLNPNSITFKTYPGMPHSACQEEMMDIKQFIEKQLPPID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RGT7</accession>
    <name>A0A2D0RGT7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Engulfment and cell motility protein 3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">elmo3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017329744.1">
        <property type="nucleotide sequence ID" value="XM_017474255.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RGT7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018380"/>
    <dbReference type="GeneID" id="108268913"/>
    <dbReference type="KEGG" id="ipu:108268913"/>
    <dbReference type="CTD" id="79767"/>
    <dbReference type="OrthoDB" id="28413at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048870">
        <property type="term" value="P:cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032835">
        <property type="term" value="P:glomerulus development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001212">
        <property type="term" value="P:regulation of vasculogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd13359">
        <property type="entry name" value="PH_ELMO1_CED-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000053">
        <property type="entry name" value="Engulfment and cell motility protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024574">
        <property type="entry name" value="ELMO_ARM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006816">
        <property type="entry name" value="ELMO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050868">
        <property type="entry name" value="ELMO_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12771">
        <property type="entry name" value="ENGULFMENT AND CELL MOTILITY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12771:SF16">
        <property type="entry name" value="ENGULFMENT AND CELL MOTILITY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11841">
        <property type="entry name" value="ELMO_ARM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04727">
        <property type="entry name" value="ELMO_CED12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16457">
        <property type="entry name" value="PH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51335">
        <property type="entry name" value="ELMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="2" id="KW-0581">Phagocytosis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0729">SH3-binding</keyword>
    <feature type="domain" description="ELMO" evidence="6">
        <location>
            <begin position="338"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="570"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017329744.1"/>
        </source>
    </evidence>
    <sequence length="746" mass="85563" checksum="848601ECCFC2BFA2" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RKM1</accession>
    <name>A0A2D0RKM1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Calponin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cnn3a</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the calponin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017331063.1">
        <property type="nucleotide sequence ID" value="XM_017475574.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RKM1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000016611"/>
    <dbReference type="GeneID" id="108269615"/>
    <dbReference type="KEGG" id="ipu:108269615"/>
    <dbReference type="CTD" id="326931"/>
    <dbReference type="OMA" id="YHSEYQD"/>
    <dbReference type="OrthoDB" id="21595at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21284">
        <property type="entry name" value="CH_CNN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000040">
        <property type="entry name" value="Calponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050606">
        <property type="entry name" value="Calponin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001997">
        <property type="entry name" value="Calponin/LIMCH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000557">
        <property type="entry name" value="Calponin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003096">
        <property type="entry name" value="SM22_calponin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385">
        <property type="entry name" value="CALPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385:SF17">
        <property type="entry name" value="CALPONIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00402">
        <property type="entry name" value="Calponin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00889">
        <property type="entry name" value="CALPONIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00888">
        <property type="entry name" value="SM22CALPONIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01052">
        <property type="entry name" value="CALPONIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51122">
        <property type="entry name" value="CALPONIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 6" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="26"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017331063.1"/>
        </source>
    </evidence>
    <sequence length="329" mass="36557" checksum="2CCED9AC1E4E3EA3" modified="2017-12-20" version="1">MTQFNKGPAYGLSAEVKSKIAQKYDMQKEEELRFWIEEVTGMPIGDNFQKGLKDGVILCELINKLQPGSIKKINHSQLNWHKLENLGNFIKAILTYGLKPNDIFEANDLFENGNMTQVQTTLLALASMAKTKGLDTKVDIGVKYADKQERHFDDDKMKAGQCVIGLQMGTNKCASQAGMTAYGTRRHLYDPKTQTDKPYDQTTISLQMGTNKGASQAGMSAPGTRRDIFDQKVAVQPLDNSTISLQMGTNKVASQKGMSVYGLGRQVYDPKYCAPATEPIIHANGSQGTGTNGSEISDSDYHVEYQEEEYQGNYHDEYSGHYNDQGIDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RLQ4</accession>
    <name>A0A2D0RLQ4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Bromodomain adjacent to zinc finger domain protein 1A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">baz1a</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WAL family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017331442.1">
        <property type="nucleotide sequence ID" value="XM_017475953.3"/>
    </dbReference>
    <dbReference type="GeneID" id="108269861"/>
    <dbReference type="KEGG" id="ipu:108269861"/>
    <dbReference type="CTD" id="11177"/>
    <dbReference type="OrthoDB" id="332390at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008623">
        <property type="term" value="C:CHRAC"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045740">
        <property type="term" value="P:positive regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031445">
        <property type="term" value="P:regulation of heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15627">
        <property type="entry name" value="PHD_BAZ1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000300">
        <property type="entry name" value="Bromodomain adjacent to zinc finger domain protein 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047171">
        <property type="entry name" value="BAZ1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018501">
        <property type="entry name" value="DDT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028942">
        <property type="entry name" value="WHIM1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028941">
        <property type="entry name" value="WHIM2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013136">
        <property type="entry name" value="WSTF_Acf1_Cbp146"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46510">
        <property type="entry name" value="BROMODOMAIN ADJACENT TO ZINC FINGER DOMAIN PROTEIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46510:SF1">
        <property type="entry name" value="BROMODOMAIN ADJACENT TO ZINC FINGER DOMAIN PROTEIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02791">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10537">
        <property type="entry name" value="WAC_Acf1_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15612">
        <property type="entry name" value="WHIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15613">
        <property type="entry name" value="WSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00571">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50827">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51136">
        <property type="entry name" value="WAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="8 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="648"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="900"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1055"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1187"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="306"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="648"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="705"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="900"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="921"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1074"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1197"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1245"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1280"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1304"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1315"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1354"/>
            <end position="1368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017331442.1"/>
        </source>
    </evidence>
    <sequence length="1490" mass="169464" checksum="65EB284636033CF6" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RLV7</accession>
    <name>A0A2D0RLV7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Multiple inositol polyphosphate phosphatase 1</fullName>
            <ecNumber evidence="4">3.1.3.62</ecNumber>
            <ecNumber evidence="3">3.1.3.80</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">2,3-bisphosphoglycerate 3-phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">minpp1a</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">SDA103</strain>
        </source>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="28">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>(2R)-2,3-bisphosphoglycerate + H2O = (2R)-2-phosphoglycerate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27381"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58248"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="3.1.3.80"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:27382"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>1D-myo-inositol 1,2,3,5,6-pentakisphosphate + H2O = 1D-myo-inositol 1,2,3,6-tetrakisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77111"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58747"/>
            <dbReference type="ChEBI" id="CHEBI:195534"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:77112"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>1D-myo-inositol 1,2,3,6-tetrakisphosphate + H2O = 1D-myo-inositol 1,2,3-trisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77123"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:195534"/>
            <dbReference type="ChEBI" id="CHEBI:195536"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:77124"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1D-myo-inositol 1,2,3-trisphosphate + H2O = 1D-myo-inositol 2,3-bisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77127"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:195536"/>
            <dbReference type="ChEBI" id="CHEBI:195538"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:77128"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>1D-myo-inositol 1,2,4,5,6-pentakisphosphate + H2O = 1D-myo-inositol 1,2,5,6-tetrakisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77115"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57798"/>
            <dbReference type="ChEBI" id="CHEBI:195535"/>
            <dbReference type="EC" id="3.1.3.62"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:77116"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1D-myo-inositol 1,2,5,6-tetrakisphosphate + H2O = 1D-myo-inositol 1,2,6-trisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77119"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:195535"/>
            <dbReference type="ChEBI" id="CHEBI:195537"/>
            <dbReference type="EC" id="3.1.3.62"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:77120"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>1D-myo-inositol 1,2,6-trisphosphate + H2O = 1D-myo-inositol 1,2-bisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77131"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:195537"/>
            <dbReference type="ChEBI" id="CHEBI:195539"/>
            <dbReference type="EC" id="3.1.3.62"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:77132"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1D-myo-inositol 1,2-bisphosphate + H2O = 1D-myo-inositol 2-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77135"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:84142"/>
            <dbReference type="ChEBI" id="CHEBI:195539"/>
            <dbReference type="EC" id="3.1.3.62"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:77136"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>1D-myo-inositol 1,3,4,5,6-pentakisphosphate + H2O = 1D-myo-inositol 1,4,5,6-tetrakisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77143"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57627"/>
            <dbReference type="ChEBI" id="CHEBI:57733"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:77144"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>1D-myo-inositol 1,4,5,6-tetrakisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77147"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57627"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:77148"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>1D-myo-inositol 2,3-bisphosphate + H2O = 1D-myo-inositol 2-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77139"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:84142"/>
            <dbReference type="ChEBI" id="CHEBI:195538"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:77140"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>1D-myo-inositol hexakisphosphate + H2O = 1D-myo-inositol 1,2,3,5,6-pentakisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20960"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:58747"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:20961"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>1D-myo-inositol hexakisphosphate + H2O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16989"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57798"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="EC" id="3.1.3.62"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:16990"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histidine acid phosphatase family. MINPP1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.62" evidence="4"/>
    <dbReference type="EC" id="3.1.3.80" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017331131.1">
        <property type="nucleotide sequence ID" value="XM_017475642.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RLV7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026585"/>
    <dbReference type="GeneID" id="108269684"/>
    <dbReference type="KEGG" id="ipu:108269684"/>
    <dbReference type="CTD" id="561891"/>
    <dbReference type="OMA" id="WCAAFTR"/>
    <dbReference type="OrthoDB" id="6509975at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034417">
        <property type="term" value="F:bisphosphoglycerate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052745">
        <property type="term" value="F:inositol phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000014">
        <property type="entry name" value="Multiple inositol polyphosphate phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016274">
        <property type="entry name" value="Histidine_acid_Pase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963:SF41">
        <property type="entry name" value="MULTIPLE INOSITOL POLYPHOSPHATE PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963">
        <property type="entry name" value="MULTIPLE INOSITOL POLYPHOSPHATE PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000894">
        <property type="entry name" value="Acid_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="25" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 26" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5011977051" description="Multiple inositol polyphosphate phosphatase 1" evidence="26">
        <location>
            <begin position="20"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="73"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="267"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00043671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00043674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00043691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00043733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00043739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00043746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00043747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00043757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00043762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00043801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00043829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00043832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR000894-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_017331131.1"/>
        </source>
    </evidence>
    <sequence length="456" mass="52071" checksum="131267BBBEEBA4D6" modified="2017-12-20" version="1">MGFLCLLVAAINLHAVSFAFPSSWCNVMSPPGPIPDNVLYFNTKSRYEEVNPYLMSDVLAINQSWVKPPSPQCRAVHLSAIIRHGTRYPTSGNIKKMIQFTKLVKNRADLSCVKELQSWKMWYKEDMDGRLVEKGRSDHRHLARRLIKAFPTLITKENLREGRVKFLTSSKHRCVNSTLAFKHGVLEGLGLQGLDLPHTIDDKLMRFFDQCQRLVETVENNKDAVREVELFKNGPEMKRVQEKLADRLQLPYINVSTDAVETAFYLCAYEFTIRSMNTPWCRLFDDADGQVLEYSGDLKQFWKRGFGHDINSKASCILFHDLFKRLDAAANQTRSGGRVSEAVMVQVGHAETLLPLLTLLDLFKDDVPLNSSNFASHHSRVFRTGQIVPYAANLLMALFNCPDGLRLQARLNERPLILPGLDELSPLYLDVRKRYEQLLQGCDQDTVCKLDSYKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RR09</accession>
    <name>A0A2D0RR09_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zinc finger and BTB domain-containing protein 7A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Leukemia/lymphoma-related factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17 18 19 20" type="primary">zbtb7a</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17 18 19" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17 18 19">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017332942.2">
        <property type="nucleotide sequence ID" value="XM_017477453.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047014065.1">
        <property type="nucleotide sequence ID" value="XM_047158109.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047014066.1">
        <property type="nucleotide sequence ID" value="XM_047158110.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047014067.1">
        <property type="nucleotide sequence ID" value="XM_047158111.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RR09"/>
    <dbReference type="GeneID" id="108270628"/>
    <dbReference type="KEGG" id="ipu:108270628"/>
    <dbReference type="CTD" id="51341"/>
    <dbReference type="GeneTree" id="ENSGT00940000166752"/>
    <dbReference type="OMA" id="MRRCQDQ"/>
    <dbReference type="OrthoDB" id="8922241at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001448">
        <property type="entry name" value="Zinc finger and BTB domain containing 7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000115">
        <property type="entry name" value="Zinc finger and BTB domain containing 7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000138">
        <property type="entry name" value="Zinc finger and BTB domain containing 7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF6">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING PROTEIN 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="14">
        <location>
            <begin position="42"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="381"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="409"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="437"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="465"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="204"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="353"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="485"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="277"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="357"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="529"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="548"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017332942.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_047014065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_047014066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_047014067.1"/>
        </source>
    </evidence>
    <sequence length="575" mass="63682" checksum="2AB7FD8631CAE428" modified="2023-02-22" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RT18</accession>
    <name>A0A2D0RT18_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Lysine-specific demethylase 4B</fullName>
            <ecNumber evidence="4">1.14.11.66</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">JmjC domain-containing histone demethylation protein 3B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Jumonji domain-containing protein 2B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">[histone H3]-trimethyl-L-lysine(9) demethylase 4B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">kdm4b</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">SDA103</strain>
        </source>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="29">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate. Plays a critical role in the development of the central nervous system (CNS).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(9)-[histone H3] + 2 2-oxoglutarate + 2 O2 = N(6)-methyl-L-lysyl(9)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:60200"/>
            <dbReference type="Rhea" id="RHEA-COMP:15538"/>
            <dbReference type="Rhea" id="RHEA-COMP:15542"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="1.14.11.66"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the JHDM3 histone demethylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.66" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017333647.1">
        <property type="nucleotide sequence ID" value="XM_017478158.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RT18"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000037342"/>
    <dbReference type="GeneID" id="108271011"/>
    <dbReference type="KEGG" id="ipu:108271011"/>
    <dbReference type="CTD" id="23030"/>
    <dbReference type="OMA" id="KFYQVEF"/>
    <dbReference type="OrthoDB" id="9547406at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140684">
        <property type="term" value="F:histone H3K9me2/H3K9me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000029">
        <property type="entry name" value="lysine-specific demethylase 4C isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000003">
        <property type="entry name" value="Lysine-specific demethylase 4D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.70:FF:000001">
        <property type="entry name" value="Putative lysine-specific demethylase 4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040477">
        <property type="entry name" value="KDM4-like_Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF7">
        <property type="entry name" value="[HISTONE H3]-TRIMETHYL-L-LYSINE(9) DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13831">
        <property type="entry name" value="PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18104">
        <property type="entry name" value="Tudor_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="23" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjN" evidence="25">
        <location>
            <begin position="60"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="26">
        <location>
            <begin position="188"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="27">
        <location>
            <begin position="747"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="482"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="999"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1049"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="23">
        <location>
            <begin position="408"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="1001"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="1050"/>
            <end position="1065"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00054423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00069270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00076122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00080011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00082446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_017333647.1"/>
        </source>
    </evidence>
    <sequence length="1105" mass="124139" checksum="2E7F1BC5D2083182" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RUS2</accession>
    <name>A0A2D0RUS2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nucleolin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ncl</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017333710.1">
        <property type="nucleotide sequence ID" value="XM_017478221.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RUS2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000036668"/>
    <dbReference type="GeneID" id="108271032"/>
    <dbReference type="KEGG" id="ipu:108271032"/>
    <dbReference type="CTD" id="4691"/>
    <dbReference type="OrthoDB" id="167718at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12404">
        <property type="entry name" value="RRM2_NCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12405">
        <property type="entry name" value="RRM3_NCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12406">
        <property type="entry name" value="RRM4_NCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000278">
        <property type="entry name" value="Nucleolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034233">
        <property type="entry name" value="Nucleolin_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034234">
        <property type="entry name" value="Nucleolin_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034235">
        <property type="entry name" value="Nucleolin_RRM4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF119">
        <property type="entry name" value="NUCLEAR RNA-BINDING PROTEIN SART-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
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        <location>
            <begin position="273"/>
            <end position="349"/>
        </location>
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    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="363"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="453"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="540"/>
            <end position="615"/>
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    </feature>
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        <location>
            <begin position="1"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="611"/>
            <end position="696"/>
        </location>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="68"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="81"/>
            <end position="91"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="96"/>
            <end position="114"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="123"/>
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        <location>
            <begin position="144"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="163"/>
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        </location>
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            <begin position="187"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
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            <begin position="222"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
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    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="620"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00017108"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017333710.1"/>
        </source>
    </evidence>
    <sequence length="696" mass="73844" checksum="8C5F224CBBB62D10" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RUU6</accession>
    <name>A0A2D0RUU6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Activating signal cointegrator 1</fullName>
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        <alternativeName>
            <fullName evidence="19">Thyroid receptor-interacting protein 4</fullName>
        </alternativeName>
    </protein>
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        <name evidence="23" type="primary">trip4</name>
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    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
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    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Transcription coactivator which associates with nuclear receptors, transcriptional coactivators including EP300, CREBBP and NCOA1, and basal transcription factors like TBP and TFIIA to facilitate nuclear receptors-mediated transcription. May thereby play an important role in establishing distinct coactivator complexes under different cellular conditions. Plays a role in thyroid hormone receptor and estrogen receptor transactivation. Also involved in androgen receptor transactivation. Plays a pivotal role in the transactivation of NF-kappa-B, SRF and AP1. Acts as a mediator of transrepression between nuclear receptor and either AP1 or NF-kappa-B. May play a role in the development of neuromuscular junction. May play a role in late myogenic differentiation. Also functions as part of the RQC trigger (RQT) complex that activates the ribosome quality control (RQC) pathway, a pathway that degrades nascent peptide chains during problematic translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with the thyroid hormone receptor/TR (via the ligand-binding domain); this interaction requires the presence of thyroid hormone. Interacts with the androgen receptor/AR; in an androgen, testosterone and dihydrotestosterone-dependent manner. Interacts with ESR1 (estrogen ligand-bound); competes with UFSP2. Interacts with UFSP2; competes with ligand-bound ESR1. Interacts with DDRGK1 and UFL1; the interaction with DDRGK1 is direct. Interacts with NCOA1. Interacts with EP300. Part of the ASC-1 complex, that contains TRIP4, ASCC1, ASCC2 and ASCC3. Identified in the RQT (ribosome quality control trigger) complex, that contains ASCC2, ASCC3 and TRIP4. Interacts with NEK6. Interacts with CSRP1. Interacts with ZCCHC4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="STRING" id="7998.ENSIPUP00000019496"/>
    <dbReference type="GeneID" id="108271295"/>
    <dbReference type="KEGG" id="ipu:108271295"/>
    <dbReference type="CTD" id="9325"/>
    <dbReference type="OMA" id="EFNSYRH"/>
    <dbReference type="OrthoDB" id="338816at2759"/>
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        <property type="component" value="Chromosome 10"/>
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    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005634">
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180022">
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="ASCH_ASC-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Hypothetical protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ASCH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056994">
        <property type="entry name" value="TRI4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039128">
        <property type="entry name" value="TRIP4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009349">
        <property type="entry name" value="TRIP4/RQT4_C2HC5_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056993">
        <property type="entry name" value="TRIP4_3rd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12963:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12963">
        <property type="entry name" value="THYROID RECEPTOR INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04266">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23135">
        <property type="entry name" value="TRI4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23134">
        <property type="entry name" value="TRIP4_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06221">
        <property type="entry name" value="zf-C2HC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01022">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ASCH" evidence="21">
        <location>
            <begin position="423"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="76"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="382"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00055901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00065803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00070627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00075052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017334264.1"/>
        </source>
    </evidence>
    <sequence length="564" mass="64573" checksum="CE635E2FBC0C309E" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RW72</accession>
    <name>A0A2D0RW72_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Neurexophilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC108271592</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May be signaling molecules that resemble neuropeptides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the neurexophilin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017334747.1">
        <property type="nucleotide sequence ID" value="XM_017479258.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RW72"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035594"/>
    <dbReference type="GeneID" id="108271592"/>
    <dbReference type="KEGG" id="ipu:108271592"/>
    <dbReference type="OMA" id="LDQTGYH"/>
    <dbReference type="OrthoDB" id="9834165at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010450">
        <property type="entry name" value="Nxph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026845">
        <property type="entry name" value="NXPH/NXPE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17103">
        <property type="entry name" value="NEUREXOPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17103:SF10">
        <property type="entry name" value="NEUREXOPHILIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06312">
        <property type="entry name" value="Neurexophilin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038019">
        <property type="entry name" value="Neurexophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012474686" description="Neurexophilin" evidence="5">
        <location>
            <begin position="22"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR038019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017334747.1"/>
        </source>
    </evidence>
    <sequence length="291" mass="32925" checksum="B13EA5341A12E50C" modified="2017-12-20" version="1">MRLLAWSFLILNQWILRKVHGLEKQVGKSLDYLELGTAGSVLKPLPYGGAGGGGIGAGGGVKPPFQTSRIFSSMDHTPMKPKPPTFGLPNPYDWARNQSHLLDQTGYHSKRKPPLKTAMKTKKIFGWGDFYFSVKTLKFNLLVTGKIVDHVNGTFTVYFRHNSSSLSNVSVSIVPPSKVVEFEVVQQQSTLHQPEIQFHQPHQSTIDPKDIKTFNCRVEYEKTNRSKKPKPCLYDPSQTCFTEHTQSNAAWLCAKPFKVICIFISFFSIDYKLVQKVCPDYNFQSEQPYFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RZ93</accession>
    <name>A0A2D0RZ93_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Histone deacetylase complex subunit SAP130a isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">sap130a</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAP130 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017335506.1">
        <property type="nucleotide sequence ID" value="XM_017480017.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RZ93"/>
    <dbReference type="GeneID" id="108271984"/>
    <dbReference type="CTD" id="100073341"/>
    <dbReference type="OrthoDB" id="10048604at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070822">
        <property type="term" value="C:Sin3-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003231">
        <property type="term" value="P:cardiac ventricle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021508">
        <property type="term" value="P:floor plate formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008345">
        <property type="term" value="P:larval locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001966">
        <property type="term" value="P:thigmotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024137">
        <property type="entry name" value="His_deAcase_cplx_SAP130"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031963">
        <property type="entry name" value="SAP130_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13497">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT SAP130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13497:SF3">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT SAP130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16014">
        <property type="entry name" value="SAP130_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase complex subunit SAP130 C-terminal" evidence="8">
        <location>
            <begin position="638"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="628"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="664"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="798"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="628"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="803"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="880"/>
            <end position="892"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017335506.1"/>
        </source>
    </evidence>
    <sequence length="1059" mass="110886" checksum="E07914F79C66D4B0" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RZZ4</accession>
    <name>A0A2D0RZZ4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">E1 ubiquitin-activating enzyme</fullName>
            <ecNumber evidence="4">6.2.1.45</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Ubiquitin-activating enzyme E1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">uba7</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="6.2.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.45" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017335756.1">
        <property type="nucleotide sequence ID" value="XM_017480267.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RZZ4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000001007"/>
    <dbReference type="GeneID" id="108272095"/>
    <dbReference type="KEGG" id="ipu:108272095"/>
    <dbReference type="CTD" id="7318"/>
    <dbReference type="OMA" id="CIREKCN"/>
    <dbReference type="OrthoDB" id="10252231at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004839">
        <property type="term" value="F:ubiquitin activating enzyme activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="P:DNA damage response"/>
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        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
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        <property type="entry name" value="SUMO-activating enzyme subunit 2"/>
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        <property type="entry name" value="ubiquitin-like modifier-activating enzyme 1"/>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
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        <property type="entry name" value="Ubiquitin-activating enzyme E1, FCCH domain"/>
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        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 1"/>
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        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 2"/>
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        <property type="entry name" value="Ubiquitin-activating enzyme E1, SCCH domain"/>
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        <property type="entry name" value="Ubiquitin-activating enzyme E1, UFD domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="E1_FCCH_sf"/>
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        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
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        <property type="entry name" value="Ub-activating_enz_E1_C"/>
    </dbReference>
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        <property type="entry name" value="Ub-E1_IAD_1"/>
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        <property type="entry name" value="UBA_E1_C_sf"/>
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        <property type="entry name" value="UBA_E1_SCCH"/>
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        <property type="entry name" value="Ubi_acti_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
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        <property type="entry name" value="UBQ-activ_enz_E1"/>
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        <property type="entry name" value="UBQ-activ_enz_E1_CS"/>
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        <property type="entry name" value="UBQ-activ_enz_E1_Cys_AS"/>
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        <property type="entry name" value="UBQ/SUMO-activ_enz_E1-like"/>
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        <property type="entry name" value="Ube1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR10953:SF198">
        <property type="entry name" value="E1 UBIQUITIN-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="E1_UFD"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ThiF"/>
        <property type="match status" value="2"/>
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    <dbReference type="Pfam" id="PF10585">
        <property type="entry name" value="UBA_E1_SCCH"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="UBIQUITINACT"/>
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        <property type="entry name" value="UBA_e1_C"/>
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        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-activating enzyme E1 C-terminal" evidence="12">
        <location>
            <begin position="883"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="10">
        <location>
            <position position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012990"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030371"/>
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    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10132"/>
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    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000519"/>
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        <source>
            <dbReference type="SMART" id="SM00985"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017335756.1"/>
        </source>
    </evidence>
    <sequence length="1015" mass="113624" checksum="52A4C7745029F8C2" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S1Y4</accession>
    <name>A0A2D0S1Y4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="26 27 28">Histone-lysine N-methyltransferase ASH1L isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="26 27 28 29" type="primary">ash1l</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">SDA103</strain>
        </source>
    </reference>
    <reference evidence="26 27 28" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26 27 28">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017336768.2">
        <property type="nucleotide sequence ID" value="XM_017481279.3"/>
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        <property type="nucleotide sequence ID" value="XM_017481280.3"/>
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        <property type="nucleotide sequence ID" value="XM_017481281.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017336771.2">
        <property type="nucleotide sequence ID" value="XM_017481282.3"/>
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        <property type="component" value="Chromosome 12"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042800">
        <property type="term" value="F:histone H3K4 methyltransferase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="BAH_polybromo"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15548">
        <property type="entry name" value="PHD_ASH1L"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SET_ASH1L"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ash1 (absent, small, or homeotic)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
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    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
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        <property type="entry name" value="Bromo_ASH1L"/>
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    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
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    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
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        <property type="entry name" value="PHD_ASH1L"/>
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        <property type="entry name" value="Post-SET_dom"/>
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        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
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        <property type="entry name" value="Znf_FYVE_PHD"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE ASH1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20826">
        <property type="entry name" value="PHD_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="7"/>
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        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S7D3</accession>
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        <name type="scientific">Ictalurus punctatus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Ostariophysi</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="19">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="20">Blood</tissue>
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            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
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        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="entry name" value="Kringle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018056">
        <property type="entry name" value="Kringle_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038178">
        <property type="entry name" value="Kringle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050127">
        <property type="entry name" value="Serine_Proteases_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264:SF40">
        <property type="entry name" value="HYALURONAN-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264">
        <property type="entry name" value="TRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00051">
        <property type="entry name" value="Kringle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00018">
        <property type="entry name" value="KRINGLE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00130">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00021">
        <property type="entry name" value="KRINGLE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50070">
        <property type="entry name" value="KRINGLE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 12" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 13" id="KW-0420">Kringle</keyword>
    <keyword evidence="5 14" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 14" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012858744" description="trypsin" evidence="15">
        <location>
            <begin position="21"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="71"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="148"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Kringle" evidence="17">
        <location>
            <begin position="191"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="18">
        <location>
            <begin position="314"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="157"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="176"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017338652.1"/>
        </source>
    </evidence>
    <sequence length="559" mass="61689" checksum="4C18F2C98A4284DC" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S872</accession>
    <name>A0A2D0S872_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ER lumen protein-retaining receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">kdelr2b</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">COPI-coated vesicle membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 14">Endoplasmic reticulum membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the ERD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017338932.2">
        <property type="nucleotide sequence ID" value="XM_017483443.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S872"/>
    <dbReference type="GeneID" id="108273834"/>
    <dbReference type="KEGG" id="ipu:108273834"/>
    <dbReference type="CTD" id="325464"/>
    <dbReference type="OMA" id="WKSRSCE"/>
    <dbReference type="OrthoDB" id="7694678at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030663">
        <property type="term" value="C:COPI-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046923">
        <property type="term" value="F:ER retention sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000133">
        <property type="entry name" value="ER_ret_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10585">
        <property type="entry name" value="ER LUMEN PROTEIN RETAINING RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00810">
        <property type="entry name" value="ER_lumen_recept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00660">
        <property type="entry name" value="ERLUMENR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00951">
        <property type="entry name" value="ER_LUMEN_RECEPTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00952">
        <property type="entry name" value="ER_LUMEN_RECEPTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12 14 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017338932.2"/>
        </source>
    </evidence>
    <sequence length="212" mass="24380" checksum="C0CE9D92A3F4B7F5" modified="2023-02-22" version="2">MNIFRLTGDLSHLAAIIILLLKIWKTRSCAGISGKSQLLFALVFTTRYLDLLTSFISLYNTTMKIIYVGCSYATVYLMYAKFKATYDGNHDTFRVEFLVVPVGGLAFLVNHDFSPLEILWTFSIYLESVAILPQLFMISKTGEAETITTHYLFFLGLYRALYLINWIWRFYFEGFFDMIAVVAGVVQTILYCDFFYLYVTKVLKGKKLSLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S994</accession>
    <name>A0A2D0S994_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Carbonic anhydrase</fullName>
            <ecNumber evidence="2 7">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC108274041</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="2 7"/>
    <dbReference type="RefSeq" id="XP_017339314.2">
        <property type="nucleotide sequence ID" value="XM_017483825.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S994"/>
    <dbReference type="GeneID" id="108274041"/>
    <dbReference type="KEGG" id="ipu:108274041"/>
    <dbReference type="OrthoDB" id="429145at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.200.10:FF:000003">
        <property type="entry name" value="Carbonic anhydrase 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018338">
        <property type="entry name" value="Carbonic_anhydrase_a-class_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF200">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00162">
        <property type="entry name" value="ALPHA_CA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039960769" description="Carbonic anhydrase" evidence="7">
        <location>
            <begin position="22"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="9">
        <location>
            <begin position="25"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="284"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="287"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017339314.2"/>
        </source>
    </evidence>
    <sequence length="342" mass="37158" checksum="5C62C7FB2A3E2EE0" modified="2023-06-28" version="2">MQMNVQLVPFLIACLLSTVYSEDSEEFCYNSPGCNATTWPIAQLYCNGTRQSPINIVTADVKSDSNLTAFSFTGFNDSSTLRNINNTGKSVIIDLEGKKMSVSGGGLQNTYTSEEFHIHWGNGSSFSGSEHTVDGKQYAMEIHILNLRSDVSVAAANDSTAYAVLGFFIEATNDSGKPESWKNLTSFLSNISKKGDSFDIMNQLTVDSLLQGVDRTKYYRYLGSRTTPPCNEIVVWTVFKDPVKVSKDLIDLFSNTVHINTTADPFITNNHRVLQALNGRVVTSQPTSSANTTSSANTTSSANTTSSANTTSSANTTSSASTLHHTLSISTALLCYVLFWCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SBN5</accession>
    <name>A0A2D0SBN5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">LOC108274466</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8 9">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017340144.1">
        <property type="nucleotide sequence ID" value="XM_017484655.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017340145.1">
        <property type="nucleotide sequence ID" value="XM_017484656.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SBN5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006417"/>
    <dbReference type="GeneID" id="108274466"/>
    <dbReference type="KEGG" id="ipu:108274466"/>
    <dbReference type="OrthoDB" id="2017893at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF519">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE MPK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="6">
        <location>
            <begin position="305"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="170"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="170"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017340144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017340145.1"/>
        </source>
    </evidence>
    <sequence length="404" mass="44545" checksum="1BF5DC0696DEC714" modified="2017-12-20" version="1">METVKKWNKDFIALDQLETKIHSKQNGMIAKNLLNTLKICNHLQLQPISRSACAIGSCPDLMMSRNEQRGFRPAPVALTPQLPPRTHRPLCLSVSSDSNGRFKALETQEWKNNLKAQMEQAHSAGAASSTGSLERASLFCASGSTTASSSGLSSPVELLGKSKSSSRFSLFSPPWNSSSESDSNPPSRTGSKKLRRRAGPGGVKTSPETPEPKPSGPEHFHYSEPVISKVTDYIYVGNVNAAYSGRMLCRNNIDSIIDMSSLPGETCLNLIPCTCSRGTKHSWSRLKVHMEESRELGEDGAELHQRCFDAINECIDASAGKRKRVLVHCRDGFSLAPTCVIQYLMIKHNMRLIAAYELLRAKHPVNIRECHQNALVSLERTLRPGGNTDPECFKQAISRKIAWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SGR2</accession>
    <name>A0A2D0SGR2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Solute carrier family 26 member 10 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">slc26a10</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="nucleotide sequence ID" value="XM_017486410.3"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
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        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
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        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00815">
        <property type="entry name" value="sulP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SpoIIaa-like"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="186"/>
        </location>
    </feature>
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        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="401"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="455"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="6">
        <location>
            <begin position="511"/>
            <end position="670"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017341899.1"/>
        </source>
    </evidence>
    <sequence length="686" mass="76104" checksum="C2C6E3BBDB4ED3BB" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SIC1</accession>
    <name>A0A2D0SIC1_ICTPU</name>
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            <fullName evidence="8">trypsin</fullName>
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        </recommendedName>
    </protein>
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        <name evidence="13" type="primary">zmp:0000001088</name>
    </gene>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="8"/>
    <dbReference type="RefSeq" id="XP_017342035.3">
        <property type="nucleotide sequence ID" value="XM_017486546.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SIC1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033993"/>
    <dbReference type="GeneID" id="108275641"/>
    <dbReference type="KEGG" id="ipu:108275641"/>
    <dbReference type="CTD" id="560086"/>
    <dbReference type="OrthoDB" id="10059102at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000077">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050127">
        <property type="entry name" value="Serine_Proteases_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264:SF20">
        <property type="entry name" value="TRYPSIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264">
        <property type="entry name" value="TRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0720">Serine protease</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039911989" description="trypsin" evidence="10">
        <location>
            <begin position="17"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="11">
        <location>
            <begin position="27"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017342035.3"/>
        </source>
    </evidence>
    <sequence length="263" mass="28127" checksum="6A5CEF91027DFBB6" modified="2023-06-28" version="3">MNLLIFLLCILLEVLAVTCQETMQGRIIGGYAPAPYSIKYIVSIQTIVGQHFCGGTLINKYWVLTAAHCNVGAGNMRIVTGDYSVSVYEGTEQYRSPQLLVPHPQYNKTTNNADIMLIKMQTPVVVNNFVSPAPLPRQGDNIAEGRVCRVSGWGFTSPTGGIPTSLHTATVPIVSSAHCNSADSYDGNITENMICAGYADGGTDACSGDSGGPLVCEGLVYGIVSWGNGCADPRYPGVYTAVSKFRKWIDNTVFGFYGNCGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SIC2</accession>
    <name>A0A2D0SIC2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">RNA-binding protein 10 isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">rbm10</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
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    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017342241.1">
        <property type="nucleotide sequence ID" value="XM_017486752.3"/>
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    <dbReference type="GeneID" id="108275778"/>
    <dbReference type="KEGG" id="ipu:108275778"/>
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    <dbReference type="OrthoDB" id="29221at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:RNA binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:mRNA splicing, via spliceosome"/>
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        <property type="entry name" value="OCRE_RBM10"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RRM1_RBM10"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RRM2_RBM10"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.30.70.330:FF:000110">
        <property type="entry name" value="RNA-binding protein 10 isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RNA-binding protein 10 isoform X2"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF7066"/>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
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        <property type="entry name" value="OCRE"/>
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        <property type="entry name" value="RBD_domain_sf"/>
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    <dbReference type="InterPro" id="IPR035618">
        <property type="entry name" value="RBM10_OCRE"/>
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        <property type="entry name" value="RBM10_RRM2"/>
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    <dbReference type="InterPro" id="IPR000504">
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        <property type="entry name" value="Znf_C2H2_type"/>
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    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
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        <property type="entry name" value="Znf_RanBP2_sf"/>
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        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13948:SF4">
        <property type="entry name" value="RNA-BINDING PROTEIN 10"/>
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        <property type="entry name" value="DUF7066"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17780">
        <property type="entry name" value="OCRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="ZF_RANBP2_1"/>
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    </dbReference>
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        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="172"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="11">
        <location>
            <begin position="255"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="337"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="764"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="110"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="551"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="631"/>
            <end position="698"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="722"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="800"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="82"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="110"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="145"/>
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        </location>
    </feature>
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        <location>
            <begin position="633"/>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="660"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="810"/>
            <end position="819"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50199"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017342241.1"/>
        </source>
    </evidence>
    <sequence length="827" mass="92635" checksum="40E11B5BC0707DE3" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SNL7</accession>
    <name>A0A2D0SNL7_ICTPU</name>
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        <recommendedName>
            <fullName evidence="14">PHD finger protein 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">PHD factor 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">phf12a</name>
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    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
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    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="13">Component of SIN3 complexes. Interacts with SIN3A in a complex composed of HDAC1, SAP30 and SIN3A. Component of the SIN3B complex, which includes SIN3B, HDAC2 or HDAC1, PHF12 and MORF4L1; interacts directly with all subunits. Interacts with TLE5.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017488810.3"/>
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    <dbReference type="OMA" id="CLKSMMK"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070822">
        <property type="term" value="C:Sin3-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22703">
        <property type="entry name" value="FHA_PHF12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15533">
        <property type="entry name" value="PHD1_PHF12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15534">
        <property type="entry name" value="PHD2_PHF12_Rco1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000154">
        <property type="entry name" value="PHD finger protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000164">
        <property type="entry name" value="PHD finger protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.60">
        <property type="entry name" value="PHD finger protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042163">
        <property type="entry name" value="PHF12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031966">
        <property type="entry name" value="PHF12_MRG-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038098">
        <property type="entry name" value="PHF12_MRG-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46309">
        <property type="entry name" value="PHD FINGER PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46309:SF1">
        <property type="entry name" value="PHD FINGER PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16737">
        <property type="entry name" value="PHF12_MRG_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="19">
        <location>
            <begin position="56"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="19">
        <location>
            <begin position="276"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="18">
        <location>
            <begin position="729"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="29"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="157"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="232"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="452"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="520"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="575"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="811"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="232"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="470"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="529"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="602"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="814"/>
            <end position="825"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00065785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00076589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017344299.1"/>
        </source>
    </evidence>
    <sequence length="929" mass="101558" checksum="3155B76111A22E89" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SQT1</accession>
    <name>A0A2D0SQT1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Dicarboxylate carrier UCP2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Mitochondrial uncoupling protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Solute carrier family 25 member 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">LOC108277060</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">SDA103</strain>
        </source>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="29">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="24">Antiporter that exports dicarboxylate intermediates of the Krebs cycle in exchange for phosphate plus a proton across the inner membrane of mitochondria, a process driven by mitochondrial motive force with an overall impact on glycolysis, glutaminolysis and glutathione-dependent redox balance. Continuous export of oxaloacetate and related four-carbon dicarboxylates from mitochondrial matrix into the cytosol negatively regulates the oxidation of acetyl-CoA substrates via the Krebs cycle, lowering the ATP/ADP ratio and reactive oxygen species (ROS) production. May mediate inducible proton entry into the mitochondrial matrix affecting ATP turnover as a protection mechanism against oxidative stress. The proton currents are most likely associated with fatty acid flipping across the inner membrane of mitochondria in a metabolic process regulated by free fatty acids and purine nucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(S)-malate(out) + phosphate(in) + H(+)(in) = (S)-malate(in) + phosphate(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:73299"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(S)-malate(out) = (S)-malate(in)</text>
            <dbReference type="Rhea" id="RHEA:74555"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>H(+)(in) = H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34979"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-aspartate(out) + phosphate(in) + H(+)(in) = L-aspartate(in) + phosphate(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:73307"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-aspartate(out) = L-aspartate(in)</text>
            <dbReference type="Rhea" id="RHEA:66332"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a long-chain fatty acid(out) = a long-chain fatty acid(in)</text>
            <dbReference type="Rhea" id="RHEA:39283"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>malonate(out) + phosphate(in) + H(+)(in) = malonate(in) + phosphate(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:73387"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15792"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>oxaloacetate(out) + phosphate(in) + H(+)(in) = oxaloacetate(in) + phosphate(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:73303"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>phosphate(in) = phosphate(out)</text>
            <dbReference type="Rhea" id="RHEA:32823"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>sulfate(out) + phosphate(in) + H(+)(in) = sulfate(in) + phosphate(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:73391"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homotetramer. Adopts an asymmetrical dimer of dimers functional form.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 27">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017344856.1">
        <property type="nucleotide sequence ID" value="XM_017489367.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SQT1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032535"/>
    <dbReference type="GeneID" id="108277060"/>
    <dbReference type="KEGG" id="ipu:108277060"/>
    <dbReference type="OMA" id="HARRYCS"/>
    <dbReference type="OrthoDB" id="448427at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000008">
        <property type="entry name" value="Mitochondrial uncoupling protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050391">
        <property type="entry name" value="Mito_Metabolite_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45618">
        <property type="entry name" value="MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00784">
        <property type="entry name" value="MTUNCOUPLING"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 26 27" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 27" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="26">
        <location>
            <begin position="11"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="26">
        <location>
            <begin position="116"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="26">
        <location>
            <begin position="215"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00035830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00050147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00050345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00050909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00051520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00052608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00054589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00074760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_017344856.1"/>
        </source>
    </evidence>
    <sequence length="312" mass="33585" checksum="237C44827DE8A588" modified="2017-12-20" version="1">MVGFGPADVPPTAAVKFIGAGTAACIADLVTFPLDTAKVRLQIQGETVGTAKAGSAQAMKYRGVFGTIATMVRTEGPRSLYNGLVAGLQRQMSFASVRIGLYDSVKQFYTKGSDHVGIGSRLLAGCTTGAMAVAMAQPTDVVKVRFQAQVSAGAANKRYHGTMDAYRTIAKEEGFRGLWKGTGPNITRNAIVNCTELVTYDLIKDALLRNTPMTDDLPCHFTSAFGAGFCTTVIASPVDVVKTRYMNSAPGQYSTALNCALAMLTKEGPMAFYKGFMPSFLRLGSWNVVMFVTYEQLKRAMMVARHNWSIPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SRF8</accession>
    <name>A0A2D0SRF8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CUE domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108277236</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017345277.1">
        <property type="nucleotide sequence ID" value="XM_017489788.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SRF8"/>
    <dbReference type="GeneID" id="108277236"/>
    <dbReference type="KEGG" id="ipu:108277236"/>
    <dbReference type="OrthoDB" id="5794653at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14366">
        <property type="entry name" value="CUE_CUED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040195">
        <property type="entry name" value="CUE_CUED1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040192">
        <property type="entry name" value="CUEDC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13467">
        <property type="entry name" value="CUE DOMAIN CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13467:SF3">
        <property type="entry name" value="CUE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00546">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017345277.1"/>
        </source>
    </evidence>
    <sequence length="383" mass="43218" checksum="FAC07A505F306137" modified="2017-12-20" version="1">MMTSLFRRSASSNGSHGSRANGNGGSELNNSKSSRQHVRRLEFNQAMDDFKTMFPSMDHEVIECVLRANNGAVDSTIDQLLQMSLDGQGTDDSSDSDDSIPPEILERTLEPDSSDEEPPPVYSPPSYEMHIYDRKHPADVPPFPPPRFEANPPTEHRQVSSYRNWNPPLLGNLPDDFLRILPQQLDSLQLSQSSLSQPSSSSSSSLSSLTQYSASEQDRKLKQYLEDERVALFLQNEEFMRELQRNRDFLIALERDRQRFESLKSQRSHSSACMERSTEGDHNASGSEEACTSISDDALFRDKLKHMGKSTRKKLFEIAKSFSEKTRRRKTKKRALLKHQSLTTAASTANLLEDVEGLTAEDEGKLRRSGAQEKDESNKEVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SVJ9</accession>
    <name>A0A2D0SVJ9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Gamma-interferon-inducible lysosomal thiol reductase</fullName>
            <ecNumber evidence="4">1.8.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Gamma-interferon-inducible protein IP-30</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC108278070</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Lysosomal thiol reductase that can reduce protein disulfide bonds. Facilitates the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Dimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the GILT family.</text>
    </comment>
    <dbReference type="EC" id="1.8.-.-" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017346704.1">
        <property type="nucleotide sequence ID" value="XM_017491215.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SVJ9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012144"/>
    <dbReference type="GeneID" id="108278070"/>
    <dbReference type="KEGG" id="ipu:108278070"/>
    <dbReference type="OMA" id="SHKEVCF"/>
    <dbReference type="OrthoDB" id="958254at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016671">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004911">
        <property type="entry name" value="Interferon-induced_GILT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234">
        <property type="entry name" value="GAMMA-INTERFERON INDUCIBLE LYSOSOMAL THIOL REDUCTASE GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234:SF43">
        <property type="entry name" value="GAMMA-INTERFERON-INDUCIBLE LYSOSOMAL THIOL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03227">
        <property type="entry name" value="GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0458">Lysosome</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012361579" description="Gamma-interferon-inducible lysosomal thiol reductase" evidence="5">
        <location>
            <begin position="23"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017346704.1"/>
        </source>
    </evidence>
    <sequence length="238" mass="25697" checksum="091B575F646EB355" modified="2017-12-20" version="1">MCVLKSALALACLAVLVYEGASSPADRCGVLGQCVQVNSSRSADSVTVSLYYESLCPGCRQFLALQLLPTFIMLNEIMNVELVPYGNAQEQKVGEKYQFTCQHGPDECLGNMIETCVLNKLKGGAQYAVIYCMEAAADVVKAAESCLALFSPETRFGDIMACANGDEGNQLMHQNAKKTAALQPPHQYVPWITVNGEHTDDLQQKAMGSLFQLVCSLYKGQAPAACTLGQKVVKTSYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SWM6</accession>
    <name>A0A2D0SWM6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein tp53i13 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">tp53i13</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017346931.1">
        <property type="nucleotide sequence ID" value="XM_017491442.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SWM6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006893"/>
    <dbReference type="GeneID" id="108278229"/>
    <dbReference type="KEGG" id="ipu:108278229"/>
    <dbReference type="CTD" id="90313"/>
    <dbReference type="OMA" id="ICMDYLI"/>
    <dbReference type="OrthoDB" id="5960270at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021454">
        <property type="entry name" value="DUF3105"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34179">
        <property type="entry name" value="TUMOR PROTEIN P53-INDUCIBLE PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34179:SF1">
        <property type="entry name" value="TUMOR PROTEIN P53-INDUCIBLE PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11303">
        <property type="entry name" value="DUF3105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013288399" evidence="3">
        <location>
            <begin position="24"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="477"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017346931.1"/>
        </source>
    </evidence>
    <sequence length="499" mass="55490" checksum="46DC760C1EB6E47D" modified="2017-12-20" version="1">MRLRAEALMWGLVWLSVLTTCHSGLTQHCDNGKAKLETDLPPPDEFLCLKQRAVTSAPQLLDISTNYSLQPAEHVCMDLPITYNHTIPNSGAHRPVGAQSGQYLYCPPQRWINNLKDGATVLLFHPCSFKDAHRSLAAVAHSCLPHFILTAHPQLSQRRPFALVSWGHTLEMSHITTTRVCEWLLMFSSNDNQTTASQSQNYNLYLTKSAPVDRALDTSVKSLKACCEEALSVYEATTRARMIRSILQHAKREMEKDDEKHSENPTPISSESDTLNPTLTQPLNNSEVKGSLSIGPHGHVTPPLHTAVHSEEDGGGVVETDEKTTATQMKRVKEPHGNTRGEETVVKLHDSQTDVKSHKMSSTMAAGKQRVKTDVAELSAGGRCTVPGHCRPPDVATAVLGGPPRGRKMTFQRTDEAVWAAAALGFLLVLLTLSVLHTRLYRNCRTPSSLYWRESQQDYESVADIIRRRLRMVGRRKRRASQSRRQECPLLSDSSTDND</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SYJ9</accession>
    <name>A0A2D0SYJ9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Retinol dehydrogenase 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">zgc:64106</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347606.1">
        <property type="nucleotide sequence ID" value="XM_017492117.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SYJ9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000016091"/>
    <dbReference type="GeneID" id="108278657"/>
    <dbReference type="KEGG" id="ipu:108278657"/>
    <dbReference type="OMA" id="CNIAIPS"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF30">
        <property type="entry name" value="RETINOL DEHYDROGENASE 11-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012632782" evidence="3">
        <location>
            <begin position="28"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017347606.1"/>
        </source>
    </evidence>
    <sequence length="323" mass="35639" checksum="BFDE473E0F6E94B8" modified="2017-12-20" version="1">MPWEMTDFISHPLWMVCTLVLAAVARAQRRGRWDPRACPVRLTGKTAIVTGANTGIGKFIALDFACRGAKVILACRSEARGMAALREIRQLSGNQNVHLRIIDTSSLESVRKFAAQILEEEKQLHILVNNAGASGLPKGITADGLEVSFATNHIGPFLLTNLLLDLLKKSAPARIVNVSSANHKRGKVDFSHFHGENLTYRMDTVYNHTKLHNVIWTNALALKLQGTGVTANSLHPGIVMTEVMRHYNWIFRLIFNIVGMFFFKSAEEGAVSSIYCAVAEETEGISGKYFDSDCSLVLPAPEARDPAIGAKEFEYCERLTAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T1X5</accession>
    <name>A0A2D0T1X5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ras-related protein Rab</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">rab32a</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The small GTPases Rab are key regulators in vesicle trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endomembrane system</location>
            <topology evidence="6">Lipid-anchor</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017348595.1">
        <property type="nucleotide sequence ID" value="XM_017493106.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053543644.1">
        <property type="nucleotide sequence ID" value="XM_053687669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T1X5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003975"/>
    <dbReference type="GeneID" id="108279117"/>
    <dbReference type="KEGG" id="ipu:108279117"/>
    <dbReference type="CTD" id="378969"/>
    <dbReference type="GeneTree" id="ENSGT00940000162477"/>
    <dbReference type="OMA" id="FCKEGGF"/>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090385">
        <property type="term" value="P:phagosome-lysosome fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04107">
        <property type="entry name" value="Rab32_Rab38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000222">
        <property type="entry name" value="RAB32, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030697">
        <property type="entry name" value="Rab29/Rab38/Rab32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981">
        <property type="entry name" value="RAB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981:SF41">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB-32 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017348595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053543644.1"/>
        </source>
    </evidence>
    <sequence length="213" mass="23825" checksum="3719555156DEF733" modified="2017-12-20" version="1">MAGGSVSECKEYLFKVLVIGELGVGKTSIIKRYVHQLFSQHYRATIGVDFALKVLNWDSKTLVRLQLWDIAGQERFGNMTRVYYKEAVGAFVVFDVTRSSTFEAVSKWKHDLDSKVKLANGNPIPSVLLANKCDQKKDSSNNASLMDNFCKEAGFLGWFETSAKDNINVDEAARFLVEHILLNDKGVPYEESNGDRIKLDQDPMPAESKSGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979E3E4</accession>
    <name>A0A979E3E4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Transmembrane protein 131-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">tmem131l</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM131 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047008208.2">
        <property type="nucleotide sequence ID" value="XM_047152252.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108260649"/>
    <dbReference type="CTD" id="23240"/>
    <dbReference type="OrthoDB" id="168404at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055435">
        <property type="entry name" value="Ig_TMEM131L_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055436">
        <property type="entry name" value="Ig_TMEM131L_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039877">
        <property type="entry name" value="TMEM131-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045695">
        <property type="entry name" value="TMEM131-like_Ig_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055437">
        <property type="entry name" value="TMEM131L_Ig_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022113">
        <property type="entry name" value="TMEM131L_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22050">
        <property type="entry name" value="RW1 PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22050:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 131-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19532">
        <property type="entry name" value="Ig_TMEM131L_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24498">
        <property type="entry name" value="Ig_TMEM131L_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24499">
        <property type="entry name" value="Ig_TMEM131L_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24501">
        <property type="entry name" value="Ig_TMEM131L_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12371">
        <property type="entry name" value="TMEM131_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Transmembrane protein 131-like N-terminal" evidence="8">
        <location>
            <begin position="110"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein 131-like second Ig-like" evidence="9">
        <location>
            <begin position="220"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="TMEM131L third Ig-like" evidence="10">
        <location>
            <begin position="408"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="TMEM131L fourth Ig-like" evidence="11">
        <location>
            <begin position="586"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="domain" description="TMEM131L fifth Ig-like" evidence="12">
        <location>
            <begin position="767"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="898"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="988"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1067"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1193"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1388"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1489"/>
            <end position="1509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="898"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="953"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="988"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1000"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1072"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1122"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1212"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1238"/>
            <end position="1251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1489"/>
            <end position="1503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF19532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF24499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF24501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_047008208.2"/>
        </source>
    </evidence>
    <sequence length="1509" mass="167982" checksum="F79B07576A5D4DFA" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979ECA7</accession>
    <name>A0A979ECA7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Mitotic-spindle organizing protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">mzt1</name>
        <name evidence="6" type="synonym">cm037</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MOZART1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047007094.1">
        <property type="nucleotide sequence ID" value="XM_047151138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979ECA7"/>
    <dbReference type="GeneID" id="100528704"/>
    <dbReference type="CTD" id="440145"/>
    <dbReference type="OrthoDB" id="48571at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000931">
        <property type="term" value="C:gamma-tubulin ring complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031021">
        <property type="term" value="C:interphase microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033566">
        <property type="term" value="P:gamma-tubulin complex localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051415">
        <property type="term" value="P:microtubule nucleation by interphase microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022214">
        <property type="entry name" value="MZT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28520">
        <property type="entry name" value="MITOTIC-SPINDLE ORGANIZING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28520:SF2">
        <property type="entry name" value="MITOTIC-SPINDLE ORGANIZING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12554">
        <property type="entry name" value="MOZART1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_047007094.1"/>
        </source>
    </evidence>
    <sequence length="84" mass="9121" checksum="48920EB96780B070" modified="2023-02-22" version="1">MASAANTNMNSVRETMDVLYPFVCPSVLLEISRLLNTGLDMESLSICVRLCEQGINPEALSSVIKELRRATDTLKASDNSTSQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EI53</accession>
    <name>A0A979EI53_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">dnal4b</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Consists of at least two heavy chains and a number of intermediate and light chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the dynein light chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047007495.1">
        <property type="nucleotide sequence ID" value="XM_047151539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EI53"/>
    <dbReference type="GeneID" id="108259448"/>
    <dbReference type="CTD" id="100005529"/>
    <dbReference type="OrthoDB" id="6506078at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21453">
        <property type="entry name" value="DLC-like_DNAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.740.10:FF:000002">
        <property type="entry name" value="Dynein light chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.740.10">
        <property type="entry name" value="Protein Inhibitor Of Neuronal Nitric Oxide Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037177">
        <property type="entry name" value="DLC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001372">
        <property type="entry name" value="Dynein_light_chain_typ-1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886:SF2">
        <property type="entry name" value="DYNEIN AXONEMAL LIGHT CHAIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01221">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01375">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54648">
        <property type="entry name" value="DLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6 12" id="KW-0243">Dynein</keyword>
    <keyword evidence="5 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_047007495.1"/>
        </source>
    </evidence>
    <sequence length="114" mass="13085" checksum="5F13D9400111D068" modified="2023-02-22" version="1">MRSCRTDAMAETAESKKEEADYKRLHSFPLIRHTDMPEEMRTETMELCVTACEKFATNNESAARMIKESMDKKFGSSWHVVIGEGFGFEITHEVKNLLYMFFGGSLAVCVWRCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F954</accession>
    <name>A0A979F954_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ferroxidase</fullName>
            <ecNumber evidence="4">1.16.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC108276644</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.16.3.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_047016593.1">
        <property type="nucleotide sequence ID" value="XM_047160637.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979F954"/>
    <dbReference type="GeneID" id="108276644"/>
    <dbReference type="KEGG" id="ipu:108276644"/>
    <dbReference type="OMA" id="PEPDMCV"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000009">
        <property type="entry name" value="Ceruloplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000028">
        <property type="entry name" value="Ceruloplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000075">
        <property type="entry name" value="hephaestin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000002">
        <property type="entry name" value="Hephaestin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF233">
        <property type="entry name" value="FERROXIDASE HEPHL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 17" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0915">Sodium</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
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    <feature type="chain" id="PRO_5037311773" description="ferroxidase" evidence="17">
        <location>
            <begin position="26"/>
            <end position="1099"/>
        </location>
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        <location>
            <begin position="100"/>
            <end position="206"/>
        </location>
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    <feature type="domain" description="Plastocyanin-like" evidence="19">
        <location>
            <begin position="470"/>
            <end position="574"/>
        </location>
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        <location>
            <begin position="800"/>
            <end position="911"/>
        </location>
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        <location>
            <begin position="968"/>
            <end position="1069"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
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        <source>
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        <source>
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            <dbReference type="Pfam" id="PF07731"/>
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        <source>
            <dbReference type="Pfam" id="PF07732"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
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    <sequence length="1099" mass="124197" checksum="949FDFE27A081F53" modified="2023-02-22" version="1"> </sequence>
</entry>
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            <fullName evidence="4">Uncharacterized protein LOC108260902</fullName>
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        <name evidence="4" type="primary">LOC108260902</name>
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        <name type="scientific">Ictalurus punctatus</name>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
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                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
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                <person name="Kucuktas H."/>
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                <person name="Liu H."/>
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                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
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    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053533375.1">
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    <dbReference type="KEGG" id="ipu:108260902"/>
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        <location>
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        <location>
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        <source>
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    </evidence>
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        <source>
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    <sequence length="259" mass="29139" checksum="A1ECF9CFCE3B8A1E" modified="2023-06-28" version="1">METSRVALIALVCVVFSGQKRISGAEVETRVRRGDDVTLYSDCVWKSGFYPVWFRNCSEKDHPRVTISLADLINSDVLGGDFTRYSFLVNNSINTHTLLIKNVSESDQGVYYCALSEKKILDNNIGVAVSVYRYGNRSTRLSVLEEATVSPNTPTPCVSEWSVSWKLVLGVCAVCVLLSSLLSSICVYCLCTNTTKETEGELAGSDKRTTWRHDEVEVEVCYASLDIQNLKKKKKKKRRVQNSDFSTYSEVRTERTEHC</sequence>
</entry>
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        <name evidence="11" type="primary">LOC128629094</name>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the alpha/beta interferon family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053531400.1">
        <property type="nucleotide sequence ID" value="XM_053675425.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QWL8"/>
    <dbReference type="GeneID" id="128629094"/>
    <dbReference type="KEGG" id="ipu:128629094"/>
    <dbReference type="OrthoDB" id="8922121at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005126">
        <property type="term" value="F:cytokine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000471">
        <property type="entry name" value="Interferon_alpha/beta/delta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11691:SF73">
        <property type="entry name" value="INTERFERON BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11691">
        <property type="entry name" value="TYPE I INTERFERON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00143">
        <property type="entry name" value="Interferon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00266">
        <property type="entry name" value="INTERFERONAB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00076">
        <property type="entry name" value="IFabd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="3 8" id="KW-0202">Cytokine</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039914820" evidence="9">
        <location>
            <begin position="25"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053531400.1"/>
        </source>
    </evidence>
    <sequence length="188" mass="21467" checksum="61AD0637F5F90C0F" modified="2023-06-28" version="1">MNHMELMKCVLLLVFVSSVCEAVALPTCQWAHYKELSEKSKNLLERMGGQLPQKCLEGKNSSFPSFPKDVFIKAQNEDLVLVALETLSGVKNIFKNNHTLVKWESDSLALFRSLVFRQVRKLQECTGKKFQTAMEKPADSSTATLRSFFEKLEERLKEKEFSSCAWEIVRTELQGGLEELQTVLESKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R0P6</accession>
    <name>A0A9F7R0P6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC108264339</name>
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    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053534114.1">
        <property type="nucleotide sequence ID" value="XM_053678139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R0P6"/>
    <dbReference type="GeneID" id="108264339"/>
    <dbReference type="KEGG" id="ipu:108264339"/>
    <dbReference type="OrthoDB" id="6581954at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005332">
        <property type="term" value="F:gamma-aminobutyric acid:sodium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11496">
        <property type="entry name" value="SLC6sbd-TauT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF237">
        <property type="entry name" value="TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="215"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="262"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="291"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="350"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="394"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="432"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="471"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="509"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053534114.1"/>
        </source>
    </evidence>
    <sequence length="564" mass="64327" checksum="89A1EFC889F81CDA" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R1E1</accession>
    <name>A0A9F7R1E1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histone H1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC128629997</name>
        <name evidence="8" type="synonym">LOC128629996</name>
        <name evidence="10" type="synonym">LOC128630098</name>
        <name evidence="11" type="synonym">LOC128630471</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9 10" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8 9 10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the histone H1/H5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053534548.1">
        <property type="nucleotide sequence ID" value="XM_053678573.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534549.1">
        <property type="nucleotide sequence ID" value="XM_053678574.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534640.1">
        <property type="nucleotide sequence ID" value="XM_053678665.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534951.1">
        <property type="nucleotide sequence ID" value="XM_053678976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R1E1"/>
    <dbReference type="GeneID" id="128629997"/>
    <dbReference type="KEGG" id="ipu:128629996"/>
    <dbReference type="KEGG" id="ipu:128629997"/>
    <dbReference type="KEGG" id="ipu:128630098"/>
    <dbReference type="KEGG" id="ipu:128630471"/>
    <dbReference type="OrthoDB" id="8964560at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00073">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000075">
        <property type="entry name" value="Histone H1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005819">
        <property type="entry name" value="H1/H5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00624">
        <property type="entry name" value="HISTONEH5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H15" evidence="6">
        <location>
            <begin position="27"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="85"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="157"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053534548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_053534549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053534640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053534951.1"/>
        </source>
    </evidence>
    <sequence length="204" mass="20724" checksum="A9A003DD93D3F97A" modified="2023-06-28" version="1">MAEVAPAPAAAPAKAPKKKAASRAKKAGPSVGELIVKAVSSSKERSGVSLAALKKALAAGGYDVEKNNSRVKIAVKGLVTKGTLVQTKGTGASGSFKLNKKQTEAKKPVKKAAPKAKKPAAKKPAATKKPKKVAAKKPAAAAKKSPKAKKPTAAAKKATKSPKKAKKPATPKKAAKSPKKAKAVKPKTTKPKAAKAKKAAPKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R1K1</accession>
    <name>A0A9F7R1K1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Stimulated by retinoic acid gene 6 protein-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC108260691</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
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                <person name="Geng X."/>
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                <person name="Sun F."/>
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                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
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                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
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                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
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                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
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                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
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                <person name="Flanagan M.E."/>
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            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053536605.1">
        <property type="nucleotide sequence ID" value="XM_053680630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R5G6"/>
    <dbReference type="GeneID" id="108266216"/>
    <dbReference type="KEGG" id="ipu:108266216"/>
    <dbReference type="CTD" id="8481"/>
    <dbReference type="OrthoDB" id="206339at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060287">
        <property type="term" value="P:epithelial cilium movement involved in determination of left/right asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055289">
        <property type="entry name" value="OFD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39063:SF1">
        <property type="entry name" value="OFD1 CENTRIOLE AND CENTRIOLAR SATELLITE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39063">
        <property type="entry name" value="ORAL-FACIAL-DIGITAL SYNDROME 1 PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16045">
        <property type="entry name" value="LisH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="668"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="759"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="809"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="889"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="234"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="377"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="594"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="713"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="759"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="811"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="892"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="908"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="921"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="945"/>
            <end position="959"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053536605.1"/>
        </source>
    </evidence>
    <sequence length="990" mass="115502" checksum="702652FA5626DC67" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RFV3</accession>
    <name>A0A9F7RFV3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histone H1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC128629647</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the histone H1/H5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053534413.1">
        <property type="nucleotide sequence ID" value="XM_053678438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RFV3"/>
    <dbReference type="GeneID" id="128629647"/>
    <dbReference type="KEGG" id="ipu:128629647"/>
    <dbReference type="OrthoDB" id="8964560at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00073">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000075">
        <property type="entry name" value="Histone H1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005819">
        <property type="entry name" value="H1/H5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00624">
        <property type="entry name" value="HISTONEH5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H15" evidence="6">
        <location>
            <begin position="27"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="82"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="153"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053534413.1"/>
        </source>
    </evidence>
    <sequence length="200" mass="20369" checksum="E12A1AE0F0E6C14B" modified="2023-06-28" version="1">MAEVAPAPAAAPAKAPKKKAASRAKKAGPSVGELIVKAVSSSKERSGVSLAALKKALAAGGYDVEKNNTRVKLAVKGLVTKGTLVQTKGTGASGSFKLNKKQTEAKKPVKKAAPKAKKPAATKKPKKVAAKKPAAAAKKSPKKAKKPAAAAKKATKSPKKAKKPATPKKAAKSPKKAKAVKPKTTKPKVAKAKKAAPKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RHD8</accession>
    <name>A0A9F7RHD8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Zinc finger protein 420</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC108272998</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053540187.1">
        <property type="nucleotide sequence ID" value="XM_053684212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RHD8"/>
    <dbReference type="GeneID" id="108272998"/>
    <dbReference type="KEGG" id="ipu:108272998"/>
    <dbReference type="OrthoDB" id="9439903at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19193">
        <property type="entry name" value="PR-SET_PRDM7_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001968">
        <property type="entry name" value="chorion transcription factor Cf2 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000966">
        <property type="entry name" value="ZFP90 zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001872">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000020">
        <property type="entry name" value="Zinc finger protein 14 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001119">
        <property type="entry name" value="zinc finger protein 408"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001343">
        <property type="entry name" value="Zinc finger protein 568"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001270">
        <property type="entry name" value="zinc finger protein 583 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000839">
        <property type="entry name" value="Zinc finger protein 691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044417">
        <property type="entry name" value="PRDM7_9_PR-SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393:SF151">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21549">
        <property type="entry name" value="PRDM2_PR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="20"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="18">
        <location>
            <begin position="170"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="316"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="412"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="439"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="467"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="495"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="523"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="551"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="579"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="737"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="765"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="793"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="821"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="849"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="877"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="905"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="933"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="961"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="989"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="1017"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="1045"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="42"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_053540187.1"/>
        </source>
    </evidence>
    <sequence length="1081" mass="124556" checksum="C6913E7AF954AAED" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RNH1</accession>
    <name>A0A9F7RNH1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cortexin domain-containing 1 protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC128634107</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="nucleotide sequence ID" value="XM_053684321.1"/>
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    <dbReference type="KEGG" id="ipu:128634107"/>
    <dbReference type="OrthoDB" id="8959371at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Cortexin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11057">
        <property type="entry name" value="Cortexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053540296.1"/>
        </source>
    </evidence>
    <sequence length="61" mass="6853" checksum="2D2D1BF5945B8CC6" modified="2023-06-28" version="1">MDHPALTRSSLEIDVDLGFALFFLFLLCLFLVITVVRCAQTVIDPYGSIPTSTYQEEQISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RU03</accession>
    <name>A0A9F7RU03_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase</fullName>
            <ecNumber evidence="11">2.7.4.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ppip5k2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Bifunctional inositol kinase that acts in concert with the IP6K kinases to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP-InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4. PP-InsP5 and (PP)2-InsP4, also respectively called InsP7 and InsP8, may regulate a variety of cellular processes, including apoptosis, vesicle trafficking, cytoskeletal dynamics, and exocytosis. Phosphorylates inositol hexakisphosphate (InsP6).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>1D-myo-inositol hexakisphosphate + ATP = 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:37459"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:74946"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:37460"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate + ATP + H(+) = 1,5-bis(diphospho)-1D-myo-inositol 2,3,4,6-tetrakisphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:10276"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58628"/>
            <dbReference type="ChEBI" id="CHEBI:77983"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:10277"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
            <location evidence="1 11">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the histidine acid phosphatase family. VIP1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.24" evidence="11"/>
    <dbReference type="RefSeq" id="XP_053542678.1">
        <property type="nucleotide sequence ID" value="XM_053686703.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108277047"/>
    <dbReference type="CTD" id="23262"/>
    <dbReference type="OrthoDB" id="18042at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033857">
        <property type="term" value="F:5-diphosphoinositol pentakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000828">
        <property type="term" value="F:inositol hexakisphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006020">
        <property type="term" value="P:inositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000003">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11950:FF:000001">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11950:FF:000003">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000013">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033379">
        <property type="entry name" value="Acid_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037446">
        <property type="entry name" value="His_Pase_VIP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040557">
        <property type="entry name" value="VIP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12750">
        <property type="entry name" value="DIPHOSPHOINOSITOL PENTAKISPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12750:SF10">
        <property type="entry name" value="INOSITOL HEXAKISPHOSPHATE AND DIPHOSPHOINOSITOL-PENTAKISPHOSPHATE KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18086">
        <property type="entry name" value="PPIP5K2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00616">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="VIP1 N-terminal" evidence="13">
        <location>
            <begin position="60"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="914"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="931"/>
            <end position="944"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF18086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_053542678.1"/>
        </source>
    </evidence>
    <sequence length="1369" mass="154312" checksum="D8C5D9B6BE42AA65" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TFM5</accession>
    <name>A0A9F7TFM5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC128629097</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC128629097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053531437.1">
        <property type="nucleotide sequence ID" value="XM_053675462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TFM5"/>
    <dbReference type="GeneID" id="128629097"/>
    <dbReference type="KEGG" id="ipu:128629097"/>
    <dbReference type="OrthoDB" id="8929197at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47306:SF2">
        <property type="entry name" value="CORE-BINDING (CB) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47306">
        <property type="entry name" value="SI:CH211-178J18.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053531437.1"/>
        </source>
    </evidence>
    <sequence length="284" mass="31531" checksum="FF8FCAFAB22A3D96" modified="2023-06-28" version="1">MPPPHNRVRLLIRDMMTVTQYQAILAVARNDFLKIFRKLVSSAQVLSTEETSYRRYCTALLVLRHFQCPAAVQGLTVSEWVNRKEVDDRVVIGFGRNETMQRAACFALTKEEEAWLQAYYSHIRPRVIRSDLNCDRFFLSSADTALLTVSNDLGVLHKAYEKSWSMGTLENFLRATSRQARGALETAAANLTDTEKEAVSSYLAYNTAVAKLRRVPTPQTVVATATLLDSLAGSDTDRFSGPSGTKSLDFAAFLMRFPASADSKAPTKLDRLKTGSGGGPHQSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TG19</accession>
    <name>A0A9F7TG19_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein cornichon homolog 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cnih3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cornichon family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053531887.1">
        <property type="nucleotide sequence ID" value="XM_053675912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TG19"/>
    <dbReference type="GeneID" id="108257624"/>
    <dbReference type="KEGG" id="ipu:108257624"/>
    <dbReference type="CTD" id="149111"/>
    <dbReference type="OrthoDB" id="434393at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003377">
        <property type="entry name" value="Cornichon"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12290">
        <property type="entry name" value="CORNICHON-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03311">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01398">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053531887.1"/>
        </source>
    </evidence>
    <sequence length="238" mass="28294" checksum="B0ED0A5BFFAC1376" modified="2023-06-28" version="1">MFTFAAFCYMLSLVLCASLIFFAIWHITAFDELHADFKVPIDQGNPFHARERLRNIERICCLLRKDTDLKCEPLKTCEPFFCWRSKATSTKYVTWTIDFAAFYEDPSMCKFHINNNVFVEFVSFFYVLGYWVTVMITVCLSFHQLVLPEYSIHGLFCIMFMCAQEWLTVGLNIPLLFYNTWRYFHSPTDTKELLYDPASVMDDDTLKFCQKEAWCKMSFFVLSFFYYLYCMIYNLVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TGG1</accession>
    <name>A0A9F7TGG1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Male-specific lethal 3 homolog isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC108258842</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053532307.1">
        <property type="nucleotide sequence ID" value="XM_053676332.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TGG1"/>
    <dbReference type="GeneID" id="108258842"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072487">
        <property type="term" value="C:MSL complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18983">
        <property type="entry name" value="CBD_MSL3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.30:FF:000003">
        <property type="entry name" value="Male-specific lethal 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000042">
        <property type="entry name" value="male-specific lethal 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.30">
        <property type="entry name" value="MRG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008676">
        <property type="entry name" value="MRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038217">
        <property type="entry name" value="MRG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026541">
        <property type="entry name" value="MRG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053820">
        <property type="entry name" value="MSL3_chromo-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10880">
        <property type="entry name" value="MORTALITY FACTOR 4-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10880:SF15">
        <property type="entry name" value="MSL COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05712">
        <property type="entry name" value="MRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22732">
        <property type="entry name" value="MSL3_chromo-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51640">
        <property type="entry name" value="MRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Chromo" evidence="7">
        <location>
            <begin position="10"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="112"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="305"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="411"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="160"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="316"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="415"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_053532307.1"/>
        </source>
    </evidence>
    <sequence length="500" mass="56329" checksum="502E1049D29E87C8" modified="2023-06-28" version="1">MNSRGMKFKFHKGEKVLCFEPDPSKAKVLYDAKVVDIVVGKDEKGKRIPKYLIHFNGWNRSWDRWAAEDHVLRDSEDNRKLQRKLARKALARMRRKGWRKRRCRLPGVNSVLKSLPEDEKEESDDPLISSSEDGDDDNSDPETIKSESESSEDLDEMKEEQEAHTKRESEEKNITINIDIPDVLKKKLEDDCYYINKRKKLVKLPCQMNIVNILESYVKHFTINAAFSANERFRHPQSSTQSNMSPHYVPPEKNEELCKEMVDGLRITFDFTLPMILLYPNEQAQFKKVSSSKFFQPIGDGAGCSTRSLGERSPSPGLNPSTPQSTDGHSAHNETSISPVITATTPKRRRCTNPDSDSTHSLRRSTRNTSGGEKSAEGGGGSSNTSPQPKRRLAEVPTPLPKFFLNLEKRTPVHSGSSSPLPLTPSKDGSGVFSGLESRRNNELNEQNNAAGHVLEKSQPFPSCRLSQCLKTQTERKLKERLTVPDTGDSEACFLKQGCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TKD9</accession>
    <name>A0A9F7TKD9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="1">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC128634184</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="1"/>
    <dbReference type="RefSeq" id="XP_053540419.1">
        <property type="nucleotide sequence ID" value="XM_053684444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TKD9"/>
    <dbReference type="GeneID" id="128634184"/>
    <dbReference type="KEGG" id="ipu:128634184"/>
    <dbReference type="OrthoDB" id="26387at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF28">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053540419.1"/>
        </source>
    </evidence>
    <sequence length="209" mass="23444" checksum="BDFD584802D1BE9E" modified="2023-06-28" version="1">MLGAVLQWAMEPSGGHWSESMLQRMVVSIKTKRECTAAAPPCEGTGHCHKETVRDKDKAERKRKAELCWKKTMTQTLESQSHFINKYIDLLTQDSEDLDASASTSAEHGPSSCDGALVCVGPRRWRARGGERRQMEMCILCHEAQEILPDGTAMVLAAFVQRSTVMSKNRKRPPHNPESCDPLFMHPDLSFGTHTGSCGHTMYSHCWQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TLT1</accession>
    <name>A0A9F7TLT1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ptprjb.1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 20">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="RefSeq" id="XP_053541904.1">
        <property type="nucleotide sequence ID" value="XM_053685929.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108274724"/>
    <dbReference type="KEGG" id="ipu:108274724"/>
    <dbReference type="CTD" id="101883510"/>
    <dbReference type="OrthoDB" id="10253954at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000009">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041201">
        <property type="entry name" value="PTPRJ_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF5">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18861">
        <property type="entry name" value="PTP_tm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="19" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039939472" description="protein-tyrosine-phosphatase" evidence="14">
        <location>
            <begin position="30"/>
            <end position="1852"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1496"/>
            <end position="1517"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="34"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="121"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="209"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="294"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="382"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="468"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="641"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="728"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="816"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="901"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="989"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="1075"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="15">
        <location>
            <begin position="1569"/>
            <end position="1825"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="16">
        <location>
            <begin position="1743"/>
            <end position="1816"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_053541904.1"/>
        </source>
    </evidence>
    <sequence length="1852" mass="197940" checksum="85E6089F7D06D840" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TN60</accession>
    <name>A0A9F7TN60_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein furry homolog isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">frya</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053543374.1">
        <property type="nucleotide sequence ID" value="XM_053687399.1"/>
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    <dbReference type="GeneID" id="108277770"/>
    <dbReference type="CTD" id="566822"/>
    <dbReference type="OrthoDB" id="6287725at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025614">
        <property type="entry name" value="Cell_morpho_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025481">
        <property type="entry name" value="Cell_Morphogen_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045842">
        <property type="entry name" value="Fry_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039867">
        <property type="entry name" value="Furry/Tao3/Mor2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029473">
        <property type="entry name" value="MOR2-PAG1_mid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12295">
        <property type="entry name" value="FURRY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12295:SF29">
        <property type="entry name" value="PROTEIN FURRY HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19421">
        <property type="entry name" value="Fry_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14225">
        <property type="entry name" value="MOR2-PAG1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14228">
        <property type="entry name" value="MOR2-PAG1_mid"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14222">
        <property type="entry name" value="MOR2-PAG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cell morphogenesis protein N-terminal" evidence="2">
        <location>
            <begin position="184"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis central region" evidence="4">
        <location>
            <begin position="1226"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis central region" evidence="4">
        <location>
            <begin position="1655"/>
            <end position="1717"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis central region" evidence="4">
        <location>
            <begin position="1798"/>
            <end position="1911"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis protein C-terminal" evidence="3">
        <location>
            <begin position="2057"/>
            <end position="2309"/>
        </location>
    </feature>
    <feature type="domain" description="Protein furry C-terminal" evidence="5">
        <location>
            <begin position="2335"/>
            <end position="3040"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1761"/>
            <end position="1804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2449"/>
            <end position="2471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2515"/>
            <end position="2549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2581"/>
            <end position="2688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1764"/>
            <end position="1782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2637"/>
            <end position="2654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF19421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053543374.1"/>
        </source>
    </evidence>
    <sequence length="3040" mass="341002" checksum="D201630662E25664" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TNH0</accession>
    <name>A0A9F7TNH0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyrosyl-DNA phosphodiesterase 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">5'-tyrosyl-DNA phosphodiesterase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC108272320</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">PML body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053539221.1">
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    </dbReference>
    <dbReference type="GeneID" id="108272320"/>
    <dbReference type="KEGG" id="ipu:108272320"/>
    <dbReference type="CTD" id="101887157"/>
    <dbReference type="OrthoDB" id="9975959at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070260">
        <property type="term" value="F:5'-tyrosyl-DNA phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09080">
        <property type="entry name" value="TDP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000024">
        <property type="entry name" value="Tyrosyl-DNA phosphodiesterase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051547">
        <property type="entry name" value="TDP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15822">
        <property type="entry name" value="TRAF AND TNF RECEPTOR-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15822:SF4">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="14">
        <location>
            <begin position="158"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="94"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="94"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_053539221.1"/>
        </source>
    </evidence>
    <sequence length="407" mass="46659" checksum="9B56C7395EFEF917" modified="2023-06-28" version="1">MEDKQNHEQSLASTEIPAGGDKESNKTSEAKIQGKVAKKKKRVRNKQRTERASDQIITPHNTYHPSDKEKQCLKPQHMPTLTCDHSTQTETLKNMSQNQATQTPSLERIHTQPTESKSTQTKPAKLSYKQLYEGQTSVDEGQDCAPDQSRNPEKLTVLTWNIDGLDPEDIRERIPSLLFHLGKYRADVVLLQELIPPYLKILKEIMTDYEFLPGSDDGYFTGILLRRCRIELVQRNIVNYPTTEMGRNLLMAEVNFLGHQLCIMTSHLESLKASSQERLNQLRRVWKRMREAPDSQTVIFGGDTNMRDWEVKKLGGLPDGITDVWEMLGEPEDCKYTWDTVNNNNKGLPFSARLRFDRVYMRAAREGTQLRPDTMTLXGLKRLVTCGRFTSDHWGILCTFSFVSSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TPW1</accession>
    <name>A0A9F7TPW1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cytoplasmic dynein 2 heavy chain 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Cytoplasmic dynein 2 heavy chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Dynein cytoplasmic heavy chain 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Dynein heavy chain isotype 1B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">LOC108277592</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">SDA103</strain>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="19">The cytoplasmic dynein complex 2 is probably composed by a heavy chain DYNC2H1 homodimer and a number of DYNC2LI1 light intermediate chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053543288.1">
        <property type="nucleotide sequence ID" value="XM_053687313.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108277592"/>
    <dbReference type="KEGG" id="ipu:108277592"/>
    <dbReference type="CTD" id="79659"/>
    <dbReference type="OrthoDB" id="10252139at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005858">
        <property type="term" value="C:axonemal dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Region D6 of dynein motor"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
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    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="2283"/>
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            <begin position="1082"/>
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            <dbReference type="ARBA" id="ARBA00022473"/>
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            <dbReference type="ARBA" id="ARBA00022475"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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            <dbReference type="ARBA" id="ARBA00022794"/>
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        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023902"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00064100"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00078768"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00083259"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00083782"/>
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    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="SMART" id="SM00382"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_053543288.1"/>
        </source>
    </evidence>
    <sequence length="4295" mass="488893" checksum="147EA5B644F20450" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TPZ5</accession>
    <name>A0A9F7TPZ5_ICTPU</name>
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        <recommendedName>
            <fullName evidence="8">Phospholipase</fullName>
            <ecNumber evidence="8">3.1.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 15 16 17" type="primary">pld1a</name>
    </gene>
    <organism evidence="13 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14 15 16" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15 16">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14445"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.4.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the phospholipase D family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.4" evidence="8"/>
    <dbReference type="RefSeq" id="XP_053543406.1">
        <property type="nucleotide sequence ID" value="XM_053687431.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053543407.1">
        <property type="nucleotide sequence ID" value="XM_053687432.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053543408.1">
        <property type="nucleotide sequence ID" value="XM_053687433.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053543409.1">
        <property type="nucleotide sequence ID" value="XM_053687434.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TPZ5"/>
    <dbReference type="GeneID" id="108278083"/>
    <dbReference type="KEGG" id="ipu:108278083"/>
    <dbReference type="CTD" id="556641"/>
    <dbReference type="OrthoDB" id="14911at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032534">
        <property type="term" value="P:regulation of microvillus assembly"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060627">
        <property type="term" value="P:regulation of vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01254">
        <property type="entry name" value="PH_PLD"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.30.1520.10:FF:000021">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000005">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000009">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000018">
        <property type="entry name" value="Phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016555">
        <property type="entry name" value="PLipase_D_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF57">
        <property type="entry name" value="PHOSPHOLIPASE D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00614">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
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    </dbReference>
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        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phospholipase_D_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PX" evidence="12">
        <location>
            <begin position="67"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="10">
        <location>
            <begin position="207"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="11">
        <location>
            <begin position="452"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="11">
        <location>
            <begin position="827"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="134"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="153"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR009376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_053543406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_053543407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_053543408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_053543409.1"/>
        </source>
    </evidence>
    <sequence length="1010" mass="116461" checksum="70F5E7B4929EF426" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TQB1</accession>
    <name>A0A9F7TQB1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein LOC108273863</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC108273863</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053541246.1">
        <property type="nucleotide sequence ID" value="XM_053685271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TQB1"/>
    <dbReference type="GeneID" id="108273863"/>
    <dbReference type="KEGG" id="ipu:108273863"/>
    <dbReference type="OrthoDB" id="9947981at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002768">
        <property type="term" value="P:immune response-regulating cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039257">
        <property type="entry name" value="BTLA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37996">
        <property type="entry name" value="B- AND T-LYMPHOCYTE ATTENUATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37996:SF1">
        <property type="entry name" value="B- AND T-LYMPHOCYTE ATTENUATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039940608" evidence="2">
        <location>
            <begin position="21"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="31"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="140"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053541246.1"/>
        </source>
    </evidence>
    <sequence length="323" mass="36646" checksum="67BB79BE8D28421B" modified="2023-06-28" version="1">MMASLIISSLLILVLLSVSANTDESHADCIPSIMVPRNTLWRARVTDLVMINCTVDTESHCWKNVDVSWCRIDNKSECRSLKHSDHMTTGRRSITESKVMLFLIFWKISMQDAGLYRCEIKSLTPTVSHAINVTVTESDPDCTPSITGPRNTVWRARVTDFLMINCTVVSESHCWKNMEVSWCRIDDKNGCRPLKHSSNIHIITELRSISDSKRTSFVIFWKISMQDAGLYRCEITSPEYTVNHTMNVTVTGTPAITVNVTDETHTPLSGEKWFYVIYFSVFVFALILAVPLVLSRGLKKFPKDEVTMTTVTYEEAEKSSVQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3TDW7</accession>
    <name>E3TDW7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cilia- and flagella-associated protein 299</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CD022</name>
        <name evidence="10" type="synonym">c22h4orf22</name>
        <name evidence="10" type="synonym">cd022</name>
        <name evidence="10" type="synonym">cfap299</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2016" name="Biol. Reprod." volume="95" first="19" last="19">
            <title>Transcriptome Display During Testicular Differentiation of Channel Catfish (Ictalurus punctatus) as Revealed by RNA-Seq Analysis.</title>
            <authorList>
                <person name="Zeng Q."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Zhang Y."/>
                <person name="Yuan Z."/>
                <person name="Jiang C."/>
                <person name="Chen A."/>
                <person name="Fu Q."/>
                <person name="Su B."/>
                <person name="Dunham R."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="27307075"/>
            <dbReference type="DOI" id="10.1095/biolreprod.116.138818"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in spermatogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GU588547">
        <property type="protein sequence ID" value="ADO28503.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187330.1">
        <property type="nucleotide sequence ID" value="NM_001200401.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TDW7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013511"/>
    <dbReference type="GeneID" id="100528131"/>
    <dbReference type="KEGG" id="ipu:100528131"/>
    <dbReference type="CTD" id="255119"/>
    <dbReference type="GeneTree" id="ENSGT00390000016547"/>
    <dbReference type="OrthoDB" id="2136125at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027887">
        <property type="entry name" value="DUF4464"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33588">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33588:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14713">
        <property type="entry name" value="DUF4464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADO28503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="NP_001187330.1"/>
        </source>
    </evidence>
    <sequence length="259" mass="29827" checksum="2DD548A123C0E05E" modified="2011-01-11" version="1">MEKDDAKTDLDEAVNEFLTYDEYLDSKITPSDLYYLEDEKLARQVVELGLRGTGEVWKREEFESRKAAAQPSRLSKRSEQNCHNIRGKGPRGRRLNVKYSLNICLRTLASTGKELKDNFLRALAEREEANRSGKNHSIIFIRDRSACGQEISGYIDYAHRLKCEDFEPYFSGKKRLLPQPTDLSFYNWKTNEVASSESANYQVIADHPTTGVLFMNKKDRNIVNVDPQASPCEQSPRMTLKSDIYLHVAIFDHYIKCTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3TF09</accession>
    <name>E3TF09_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">5-formyltetrahydrofolate cyclo-ligase</fullName>
            <ecNumber evidence="5 7">6.3.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MTHFS</name>
        <name evidence="10" type="synonym">mthfs</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="journal article" date="2016" name="Biol. Reprod." volume="95" first="19" last="19">
            <title>Transcriptome Display During Testicular Differentiation of Channel Catfish (Ictalurus punctatus) as Revealed by RNA-Seq Analysis.</title>
            <authorList>
                <person name="Zeng Q."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Zhang Y."/>
                <person name="Yuan Z."/>
                <person name="Jiang C."/>
                <person name="Chen A."/>
                <person name="Fu Q."/>
                <person name="Su B."/>
                <person name="Dunham R."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="27307075"/>
            <dbReference type="DOI" id="10.1095/biolreprod.116.138818"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="5">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 7">
            <text>(6S)-5-formyl-5,6,7,8-tetrahydrofolate + ATP = (6R)-5,10-methenyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10488"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the 5-formyltetrahydrofolate cyclo-ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.2" evidence="5 7"/>
    <dbReference type="EMBL" id="GU588942">
        <property type="protein sequence ID" value="ADO28895.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187620.1">
        <property type="nucleotide sequence ID" value="NM_001200691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TF09"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034516"/>
    <dbReference type="GeneID" id="100528421"/>
    <dbReference type="KEGG" id="ipu:100528421"/>
    <dbReference type="CTD" id="10588"/>
    <dbReference type="OrthoDB" id="2015992at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030272">
        <property type="term" value="F:5-formyltetrahydrofolate cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009396">
        <property type="term" value="P:folic acid-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10420:FF:000007">
        <property type="entry name" value="5-formyltetrahydrofolate cyclo-ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02727">
        <property type="entry name" value="MTHFS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407:SF1">
        <property type="entry name" value="5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407">
        <property type="entry name" value="ATPASE INHIBITOR/5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01812">
        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006806">
        <property type="entry name" value="FTHF_cligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="8"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="144"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00036539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006806-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADO28895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="NP_001187620.1"/>
        </source>
    </evidence>
    <sequence length="199" mass="22777" checksum="BDD1CE585CB4D3C4" modified="2011-01-11" version="1">MAVLRAAKQALRKEIKKRVAALSDEEKLRQSQVVSRKLFKHHNYRSSHRVAVFLSMHDEVRAEAILQHMFSSGKVCFIPRYQSDSNHMDMLQLNSMEDLNSLPITSWNIRQPADNDTQREEALATGGLDLILMPGLGFDKNGNRLGRGKGFYDLYLQRCMRHSKGKPYTIALAFKEQLCEQIPVDDTDILIDEVLCEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-07-05" modified="2026-01-28" version="97" xmlns="https://uniprot.org/uniprot">
    <accession>Q6WVG1</accession>
    <name>Q6WVG1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9 11">Interferon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">IFN</name>
        <name evidence="11" type="synonym">ifn</name>
        <name evidence="11" type="synonym">ifnphia1</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2003-04" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Long S.M."/>
                <person name="Wilson M.R."/>
                <person name="Bengten E."/>
                <person name="Bryan L.J."/>
                <person name="Clem L.W."/>
                <person name="Miller N.W."/>
                <person name="Chinchar V.G."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="8 11" key="2">
        <citation type="journal article" date="2004" name="Dev. Comp. Immunol." volume="28" first="97" last="111">
            <title>Identification of a cDNA encoding channel catfish interferon.</title>
            <authorList>
                <person name="Long S."/>
                <person name="Wilson M."/>
                <person name="Bengten E."/>
                <person name="Bryan L."/>
                <person name="Clem L.W."/>
                <person name="Miller N.W."/>
                <person name="Chinchar V.G."/>
            </authorList>
            <dbReference type="PubMed" id="12969796"/>
            <dbReference type="DOI" id="10.1016/S0145-305X(03)00122-8"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="9 11" key="3">
        <citation type="journal article" date="2006" name="Fish Shellfish Immunol." volume="21" first="42" last="59">
            <title>Identification and expression analysis of cDNAs encoding channel catfish type I interferons.</title>
            <authorList>
                <person name="Long S."/>
                <person name="Milev-Milovanovic I."/>
                <person name="Wilson M."/>
                <person name="Bengten E."/>
                <person name="Clem L.W."/>
                <person name="Miller N.W."/>
                <person name="Chinchar V.G."/>
            </authorList>
            <dbReference type="PubMed" id="16426862"/>
            <dbReference type="DOI" id="10.1016/j.fsi.2005.10.008"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="4">
        <citation type="journal article" date="2012" name="Dev. Comp. Immunol." volume="38" first="169" last="180">
            <title>Transcriptomic signatures of attachment, NF-kappaB suppression and IFN stimulation in the catfish gill following columnaris bacterial infection.</title>
            <authorList>
                <person name="Sun F."/>
                <person name="Peatman E."/>
                <person name="Li C."/>
                <person name="Liu S."/>
                <person name="Jiang Y."/>
                <person name="Zhou Z."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="22669032"/>
            <dbReference type="DOI" id="10.1016/j.dci.2012.05.006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="10" key="5">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="6">
        <citation type="journal article" date="2022" name="Fish Shellfish Immunol." volume="123" first="442" last="452">
            <title>Interferons and interferon receptors in the channel catfish, Ictalurus punctatus.</title>
            <authorList>
                <person name="Quiniou S.M.A."/>
                <person name="Crider J."/>
                <person name="Felch K.L."/>
                <person name="Bengten E."/>
                <person name="Boudinot P."/>
            </authorList>
            <dbReference type="PubMed" id="35304241"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="7">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alpha/beta interferon family.</text>
    </comment>
    <dbReference type="EMBL" id="AY267538">
        <property type="protein sequence ID" value="AAP92146.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AY847297">
        <property type="protein sequence ID" value="AAV97701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187107.1">
        <property type="nucleotide sequence ID" value="NM_001200178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q6WVG1"/>
    <dbReference type="SMR" id="Q6WVG1"/>
    <dbReference type="GeneID" id="100304604"/>
    <dbReference type="KEGG" id="ipu:100304604"/>
    <dbReference type="CTD" id="100304604"/>
    <dbReference type="OrthoDB" id="8924072at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005126">
        <property type="term" value="F:cytokine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0000315"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043330">
        <property type="term" value="P:response to exogenous dsRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060337">
        <property type="term" value="P:type I interferon-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0000315"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000471">
        <property type="entry name" value="Interferon_alpha/beta/delta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11691:SF73">
        <property type="entry name" value="INTERFERON BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11691">
        <property type="entry name" value="TYPE I INTERFERON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00143">
        <property type="entry name" value="Interferon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAP92146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAV97701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="NP_001187107.1"/>
        </source>
    </evidence>
    <sequence length="162" mass="18978" checksum="B9D26047903D906D" modified="2004-07-05" version="1">MISQYRAKNNFCVSLLKEMGGEIVPVNSSFPCEEYTEIEKAKAEDQVRFLAQATKQIISLFNAVSKVDEVKWDRRALDDFLNILNTRQLTELTKCTSTYAKRGHSPTERKLRKRFKELKKLLREAKYSADSLERIRNVVQRDLWRMDIIAANVKHKLLKRTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-12-01" modified="2026-01-28" version="95" xmlns="https://uniprot.org/uniprot">
    <accession>Q90YR9</accession>
    <name>Q90YR9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">rps5</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12" key="1">
        <citation type="journal article" date="2002" name="Gene" volume="291" first="177" last="186">
            <title>Translational machinery of channel catfish: I. A transcriptomic approach to the analysis of 32 40S ribosomal protein genes and their expression.</title>
            <authorList>
                <person name="Karsi A."/>
                <person name="Patterson A."/>
                <person name="Feng J."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="12095691"/>
            <dbReference type="DOI" id="10.1016/S0378-1119(02)00595-4"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the small ribosomal subunit. Part of the small subunit (SSU) processome, composed of more than 70 proteins and the RNA chaperone small nucleolar RNA (snoRNA) U3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="AF402813">
        <property type="protein sequence ID" value="AAK95187.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187070.1">
        <property type="nucleotide sequence ID" value="NM_001200141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q90YR9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007024"/>
    <dbReference type="GeneID" id="100304559"/>
    <dbReference type="KEGG" id="ipu:100304559"/>
    <dbReference type="CTD" id="6193"/>
    <dbReference type="GeneTree" id="ENSGT00390000010806"/>
    <dbReference type="OMA" id="AMKHATY"/>
    <dbReference type="OrthoDB" id="10264639at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14867">
        <property type="entry name" value="uS7_Eukaryote"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.455.10:FF:000003">
        <property type="entry name" value="40S ribosomal protein S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_uS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020606">
        <property type="entry name" value="Ribosomal_uS7_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_uS7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005716">
        <property type="entry name" value="Ribosomal_uS7_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003106">
        <property type="entry name" value="PRK04027.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01028">
        <property type="entry name" value="uS7_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00052">
        <property type="entry name" value="RIBOSOMAL_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 8 10" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS7" evidence="9">
        <location>
            <begin position="44"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAK95187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="NP_001187070.1"/>
        </source>
    </evidence>
    <sequence length="203" mass="22761" checksum="6797B2005C87D650" modified="2001-12-01" version="1">MADWETAPAVAETPEIKLFGKWSTDDVQINDISLQDYIAVKEKYAKYLPHSGGRYAAKRFRKAQCPIVERLTNSMMMHGRNNGKKLMTVRIVKHAFEIIHLLTGENPLQVLVNAIINSGPREDSTRIGRAGTVRRQAVDVSPLRRVNQAIWLLCTGAREAAFRNIKTIAECLADELINAAKGSSNSYAIKKKDELERVAKSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5U7Y6</accession>
    <accession>A0A2D0SZ98</accession>
    <name>W5U7Y6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="25 26">SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4a isoform X1</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="23">Transcription activator BRG1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">SMARCA4</name>
        <name evidence="25 26" type="synonym">smarca4a</name>
    </gene>
    <organism evidence="23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">SDA103</strain>
        </source>
    </reference>
    <reference evidence="25 26" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25 26">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="JT407207">
        <property type="protein sequence ID" value="AHH37900.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017348003.2">
        <property type="nucleotide sequence ID" value="XM_017492514.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053530601.1">
        <property type="nucleotide sequence ID" value="XM_053674626.1"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000009202"/>
    <dbReference type="GeneID" id="108278850"/>
    <dbReference type="KEGG" id="ipu:108278850"/>
    <dbReference type="CTD" id="353295"/>
    <dbReference type="GeneTree" id="ENSGT00940000156887"/>
    <dbReference type="OrthoDB" id="6017at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05516">
        <property type="entry name" value="Bromo_SNF2L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000008">
        <property type="entry name" value="Chromatin structure-remodeling complex subunit snf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000004">
        <property type="entry name" value="probable global transcription activator SNF2L2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.5.120:FF:000001">
        <property type="entry name" value="probable global transcription activator SNF2L2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000089">
        <property type="entry name" value="Putative global transcription activator SNF2L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003020">
        <property type="entry name" value="SNF2-related domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006576">
        <property type="entry name" value="BRK_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037259">
        <property type="entry name" value="BRK_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014978">
        <property type="entry name" value="Gln-Leu-Gln_QLQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014012">
        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029295">
        <property type="entry name" value="SnAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07533">
        <property type="entry name" value="BRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07529">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08880">
        <property type="entry name" value="QLQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14619">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00592">
        <property type="entry name" value="BRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00573">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00951">
        <property type="entry name" value="QLQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01314">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160481">
        <property type="entry name" value="BRK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51204">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51666">
        <property type="entry name" value="QLQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="10 15" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="QLQ" evidence="22">
        <location>
            <begin position="197"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="HSA" evidence="21">
        <location>
            <begin position="479"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="18">
        <location>
            <begin position="587"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="19">
        <location>
            <begin position="780"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="20">
        <location>
            <begin position="1098"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="17">
        <location>
            <begin position="1460"/>
            <end position="1530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="232"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="597"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="666"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1342"/>
            <end position="1444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1547"/>
            <end position="1629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="74"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="132"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="179"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="279"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="322"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
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            <begin position="598"/>
            <end position="607"/>
        </location>
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        <location>
            <begin position="677"/>
            <end position="691"/>
        </location>
    </feature>
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        <location>
            <begin position="1354"/>
            <end position="1374"/>
        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="16">
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            <begin position="1387"/>
            <end position="1396"/>
        </location>
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        <location>
            <begin position="1413"/>
            <end position="1422"/>
        </location>
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        <location>
            <begin position="1550"/>
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            <end position="1619"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
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            <dbReference type="ARBA" id="ARBA00022902"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022990"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
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    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50014"/>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51192"/>
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    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="AHH37900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_017348003.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_053530601.1"/>
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    <sequence length="1629" mass="182937" checksum="10A9749C5F1FA731" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5UCA8</accession>
    <name>W5UCA8_ICTPU</name>
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        <recommendedName>
            <fullName evidence="3">Erythropoietin receptor</fullName>
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    <gene>
        <name evidence="17" type="primary">EPOR</name>
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    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
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    <comment type="similarity">
        <text evidence="2">Belongs to the type I cytokine receptor family. Type 1 subfamily.</text>
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        <property type="component" value="Chromosome 2"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Erythropoietin_rcpt"/>
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        <property type="entry name" value="FN3_dom"/>
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        <property type="entry name" value="FN3_sf"/>
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        <property type="entry name" value="Growth/epo_recpt_lig-bind"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ERYTHROPOIETIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EpoR_lig-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EPO_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
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    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 17 19" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540151" description="Erythropoietin receptor" evidence="15">
        <location>
            <begin position="23"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="16">
        <location>
            <begin position="138"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="325"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007885"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018355"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001959-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AHH37302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017314843.1"/>
        </source>
    </evidence>
    <sequence length="529" mass="59998" checksum="2C205A8508C33131" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5UDE5</accession>
    <name>W5UDE5_ICTPU</name>
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        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS9m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">28S ribosomal protein S9, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MRPS9</name>
        <name evidence="13" type="synonym">mrps9</name>
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    <organism evidence="11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT406757">
        <property type="protein sequence ID" value="AHH37712.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017337578.1">
        <property type="nucleotide sequence ID" value="XM_017482089.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UDE5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018257"/>
    <dbReference type="GeneID" id="108273090"/>
    <dbReference type="KEGG" id="ipu:108273090"/>
    <dbReference type="CTD" id="64965"/>
    <dbReference type="OMA" id="HHFLFYT"/>
    <dbReference type="OrthoDB" id="10254627at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000035">
        <property type="entry name" value="28S ribosomal protein S9, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023035">
        <property type="entry name" value="Ribosomal_uS9_bac/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_uS9_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001099">
        <property type="entry name" value="PRK00132.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US9M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 9 11" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="361"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00076042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AHH37712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017337578.1"/>
        </source>
    </evidence>
    <sequence length="385" mass="44198" checksum="723E5FAF9153A16B" modified="2014-04-16" version="1">MAASSSRAVRTIFSKCAKCSWSLFISTPNMNRQLISRQICVSSAAWRKNLAAAGPEKFSEEYIKKQIDEFNIGKRHLANMMGEDPETFTQEDIDRCIAYLFPSGLFEKGARPIMKHPDLIFPKQAAVQWGEDGRPFHYLFYTGKQSYYSLMHDMFGKVLAIEKYQDKLRSKGLFTQDTKQISLSGSRWLLKEELQQLLVENISDHDYARFLQLMERLLSMPYCSMEEEFVQRFRRKLEVQSSQQEIPEIQHDEEGVAFSQADGRRKSSKASVTLRDRGSGHITINGSNYMQYFPILQDREQLMFPLHFVDMLERFDVEASVEGGGHSSKAGALRLAISRALLSFVSNGDVENMRQAGLLTSDPRVKERKKPGQAGARKKFTWKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5UDM9</accession>
    <name>W5UDM9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">cAMP-dependent protein kinase type I-alpha regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">PRKAR1A</name>
        <name evidence="18" type="synonym">prkar1ab</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cAMP-dependent kinase regulatory chain family.</text>
    </comment>
    <dbReference type="EMBL" id="JT411969">
        <property type="protein sequence ID" value="AHH39679.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017339311.1">
        <property type="nucleotide sequence ID" value="XM_017483822.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UDM9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020870"/>
    <dbReference type="GeneID" id="108274040"/>
    <dbReference type="KEGG" id="ipu:108274040"/>
    <dbReference type="CTD" id="550427"/>
    <dbReference type="GeneTree" id="ENSGT00940000155148"/>
    <dbReference type="OMA" id="DQWERAN"/>
    <dbReference type="OrthoDB" id="417078at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd12101">
        <property type="entry name" value="DD_RIalpha_PKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000013">
        <property type="entry name" value="cAMP-dependent protein kinase type I regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.890.10:FF:000001">
        <property type="entry name" value="cAMP-dependent protein kinase type I-alpha regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000006">
        <property type="entry name" value="cAMP-dependent protein kinase type I-alpha regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.890.10">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012198">
        <property type="entry name" value="cAMP_dep_PK_reg_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003117">
        <property type="entry name" value="cAMP_dep_PK_reg_su_I/II_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF129">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I-ALPHA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02197">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000548">
        <property type="entry name" value="PK_regulatory"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00103">
        <property type="entry name" value="CAMPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00394">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47391">
        <property type="entry name" value="Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10 13" id="KW-0114">cAMP</keyword>
    <keyword evidence="5 13" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="15">
        <location>
            <begin position="132"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="15">
        <location>
            <begin position="250"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="61"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000548-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AHH39679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017339311.1"/>
        </source>
    </evidence>
    <sequence length="376" mass="42838" checksum="61B0E5B06226825A" modified="2014-04-16" version="1">MASSSEEERSLRECELYVQKHNIQQLLKDCIVQLCTARPERPMAFLREYFERLEKEEAKQLINQQKASSRSDSREDEVSPPMNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVTYIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWATSIGEGGSFGELALIYGTPRAATVRAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDSQKIVVQGEPGDEFFIILEGCAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5UEY2</accession>
    <name>W5UEY2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein FAM101A</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="9">Refilin-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">FAM101A</name>
        <name evidence="9" type="synonym">rflna</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Interacts with FLNA and FLNB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Refilin family.</text>
    </comment>
    <dbReference type="EMBL" id="JT408367">
        <property type="protein sequence ID" value="AHH38321.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JT414167">
        <property type="protein sequence ID" value="AHH40523.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017323213.1">
        <property type="nucleotide sequence ID" value="XM_017467724.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UEY2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007546"/>
    <dbReference type="GeneID" id="108265428"/>
    <dbReference type="KEGG" id="ipu:108265428"/>
    <dbReference type="CTD" id="144347"/>
    <dbReference type="GeneTree" id="ENSGT00390000016836"/>
    <dbReference type="OMA" id="AKDGKVM"/>
    <dbReference type="OrthoDB" id="9946281at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031005">
        <property type="term" value="F:filamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061572">
        <property type="term" value="P:actin filament bundle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900158">
        <property type="term" value="P:negative regulation of bone mineralization involved in bone maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061182">
        <property type="term" value="P:negative regulation of chondrocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048705">
        <property type="term" value="P:skeletal system morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028215">
        <property type="entry name" value="Refilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31848">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31848:SF0">
        <property type="entry name" value="REFILIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15068">
        <property type="entry name" value="FAM101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="14"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AHH40523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017323213.1"/>
        </source>
    </evidence>
    <sequence length="219" mass="25038" checksum="6D8EB9A547368050" modified="2014-04-16" version="1">MVGHLHLQAMEESLKGKSREGLLDSPDSGLPPSPSPPYCPLSPSTTEPRTPITADHHGGMEWRKENREGKLLPYLLLNSQGADVKSRMHPVVFGESIEVNPRPTTEMRFSSEVKYDSHRHYRDEVYCARVPTVTSYSETVVALQNHTWRSYKSEVHFLPRHRPVHYQSTAIVYPKHARNTYRTTLHYNANAAGRRRFVSTVRLESSEDGSPRIIYSEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5UIG6</accession>
    <name>W5UIG6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Matrix metalloproteinase-14</fullName>
            <ecNumber evidence="6">3.4.24.80</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="33" type="primary">MMP15</name>
        <name evidence="35" type="synonym">mmp15a</name>
    </gene>
    <organism evidence="33">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="33">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="34" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="34">SDA103</strain>
        </source>
    </reference>
    <reference evidence="35" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="35">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endopeptidase activity. Activates progelatinase A by cleavage of the propeptide at 37-Asn-|-Leu-38. Other bonds hydrolyzed include 35-Gly-|-Ile-36 in the propeptide of collagenase 3, and 341-Asn-|-Phe-342, 441-Asp-|-Leu-442 and 354-Gln-|-Thr-355 in the aggrecan interglobular domain.</text>
            <dbReference type="EC" id="3.4.24.80"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="25">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="25">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="25">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="25">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Melanosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.80" evidence="6"/>
    <dbReference type="EMBL" id="JT411516">
        <property type="protein sequence ID" value="AHH39467.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017330439.1">
        <property type="nucleotide sequence ID" value="XM_017474950.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UIG6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018582"/>
    <dbReference type="MEROPS" id="M10.016"/>
    <dbReference type="GeneID" id="108269224"/>
    <dbReference type="KEGG" id="ipu:108269224"/>
    <dbReference type="CTD" id="561041"/>
    <dbReference type="GeneTree" id="ENSGT00940000156939"/>
    <dbReference type="OMA" id="VWGSIPA"/>
    <dbReference type="OrthoDB" id="406838at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048870">
        <property type="term" value="P:cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000005">
        <property type="entry name" value="Matrix metallopeptidase 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.110.10.10:FF:000001">
        <property type="entry name" value="Matrix metallopeptidase 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.101.10:FF:000002">
        <property type="entry name" value="Matrix metalloproteinase-14 preproprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Hemopexin-like domain"/>
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        <property type="entry name" value="Hemopexin-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
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        <property type="entry name" value="Hemopexin_CS"/>
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    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
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    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
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        <property type="entry name" value="Pept_M10A"/>
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        <property type="entry name" value="Peptidase_Metallo"/>
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    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
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    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
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    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF25">
        <property type="entry name" value="MATRIX METALLOPROTEINASE-15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11857">
        <property type="entry name" value="DUF3377"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="4"/>
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        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MATRIXIN"/>
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    <dbReference type="SMART" id="SM00120">
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        <property type="match status" value="4"/>
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    <dbReference type="SUPFAM" id="SSF55486">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00024">
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    <dbReference type="PROSITE" id="PS51642">
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        <property type="match status" value="4"/>
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    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="23" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="21 30" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 25" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20 35" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0645">Protease</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0677">Repeat</keyword>
    <keyword evidence="14 31" id="KW-0732">Signal</keyword>
    <keyword evidence="12 30" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="19 30" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="17 25" id="KW-0862">Zinc</keyword>
    <keyword evidence="22" id="KW-0865">Zymogen</keyword>
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        <location>
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        </location>
    </feature>
    <feature type="chain" id="PRO_5013540302" description="Matrix metalloproteinase-14" evidence="31">
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            <begin position="28"/>
            <end position="665"/>
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        <location>
            <begin position="617"/>
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    </feature>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="32">
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            <begin position="114"/>
            <end position="283"/>
        </location>
    </feature>
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        <location>
            <begin position="362"/>
            <end position="410"/>
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        <location>
            <begin position="411"/>
            <end position="456"/>
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            <begin position="457"/>
            <end position="505"/>
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        <location>
            <begin position="506"/>
            <end position="553"/>
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    </feature>
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            <begin position="283"/>
            <end position="363"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="560"/>
            <end position="605"/>
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            <begin position="90"/>
            <end position="106"/>
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        <location>
            <begin position="288"/>
            <end position="318"/>
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    </feature>
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        <location>
            <begin position="327"/>
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        <location>
            <begin position="560"/>
            <end position="579"/>
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        <location>
            <begin position="580"/>
            <end position="603"/>
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            <position position="238"/>
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            <position position="92"/>
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        <ligand>
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            <note>catalytic</note>
        </ligand>
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    <feature type="binding site" evidence="25">
        <location>
            <position position="174"/>
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        <ligand>
            <name>Ca(2+)</name>
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        </ligand>
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    <feature type="binding site" evidence="25">
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        </ligand>
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        <location>
            <position position="186"/>
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        <ligand>
            <name>Zn(2+)</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
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            <position position="191"/>
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        </ligand>
    </feature>
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        <location>
            <position position="192"/>
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        </ligand>
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        <location>
            <position position="199"/>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
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            <position position="208"/>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="212"/>
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        <ligand>
            <name>Zn(2+)</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="214"/>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="217"/>
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        <ligand>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="217"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="237"/>
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        <ligand>
            <name>Zn(2+)</name>
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            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="241"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="247"/>
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            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="372"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="415"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="463"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphotyrosine; by PKDCC" evidence="26">
        <location>
            <position position="445"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00001718"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004223"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
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    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
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    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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        <source>
            <dbReference type="PIRSR" id="PIRSR621190-1"/>
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    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-2"/>
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    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-4"/>
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    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-5"/>
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    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01011"/>
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    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="EMBL" id="AHH39467.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="RefSeq" id="XP_017330439.1"/>
        </source>
    </evidence>
    <sequence length="665" mass="75855" checksum="6FA244BE8A7D8D33" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5UIM2</accession>
    <name>W5UIM2_ICTPU</name>
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        <recommendedName>
            <fullName evidence="21 25">Cystathionine beta-synthase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">CBS</name>
        <name evidence="30" type="synonym">cbsa</name>
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    <organism evidence="28">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="28">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="29" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="29">SDA103</strain>
        </source>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="30">Blood</tissue>
        </source>
    </reference>
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        <text evidence="22">Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine. Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23 25">
            <text>L-homocysteine + L-serine = L,L-cystathionine + H2O</text>
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            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
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            <dbReference type="ChEBI" id="CHEBI:58199"/>
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    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 25">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 25">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
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    <dbReference type="EMBL" id="JT416037">
        <property type="protein sequence ID" value="AHH41907.1"/>
        <property type="molecule type" value="mRNA"/>
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        <property type="nucleotide sequence ID" value="XM_017458119.3"/>
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    <dbReference type="AlphaFoldDB" id="W5UIM2"/>
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    <dbReference type="GeneTree" id="ENSGT00510000047027"/>
    <dbReference type="OMA" id="KFADDEW"/>
    <dbReference type="OrthoDB" id="728at2759"/>
    <dbReference type="UniPathway" id="UPA00136">
        <property type="reaction ID" value="UER00201"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004122">
        <property type="term" value="F:cystathionine beta-synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0019343">
        <property type="term" value="P:cysteine biosynthetic process via cystathionine"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0050667">
        <property type="term" value="P:homocysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04608">
        <property type="entry name" value="CBS_pair_CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000014">
        <property type="entry name" value="Cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000003">
        <property type="entry name" value="Cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000118">
        <property type="entry name" value="Related to CYS4-cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046353">
        <property type="entry name" value="CBS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005857">
        <property type="entry name" value="Cysta_beta_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01137">
        <property type="entry name" value="cysta_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="11 25" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="17 24 25" id="KW-0129">CBS domain</keyword>
    <keyword evidence="18 25" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0349">Heme</keyword>
    <keyword evidence="16" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="19 25" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15 25" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="CBS" evidence="27">
        <location>
            <begin position="421"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="26">
        <location>
            <begin position="23"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00026192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00045425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU361204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="EMBL" id="AHH41907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="RefSeq" id="XP_017313608.1"/>
        </source>
    </evidence>
    <sequence length="563" mass="62110" checksum="EDB0B88979E24C58" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5UIP0</accession>
    <name>W5UIP0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13 15">Sodium/glucose cotransporter 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13 15" type="primary">slc5a9</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="JT416113">
        <property type="protein sequence ID" value="AHH41932.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017324164.1">
        <property type="nucleotide sequence ID" value="XM_017468675.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UIP0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000001807"/>
    <dbReference type="GeneID" id="108265873"/>
    <dbReference type="KEGG" id="ipu:108265873"/>
    <dbReference type="CTD" id="200010"/>
    <dbReference type="OMA" id="WKRMTPT"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005412">
        <property type="term" value="F:D-glucose:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1730.10:FF:000004">
        <property type="entry name" value="sodium/glucose cotransporter 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018212">
        <property type="entry name" value="Na/solute_symporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11819:SF96">
        <property type="entry name" value="SODIUM_GLUCOSE COTRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11819">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00456">
        <property type="entry name" value="NA_SOLUT_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00457">
        <property type="entry name" value="NA_SOLUT_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="145"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="175"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="415"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="448"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="477"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="519"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="633"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AHH41932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017324164.1"/>
        </source>
    </evidence>
    <sequence length="657" mass="72363" checksum="47481FCEB975CF0E" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>W5UKA9</accession>
    <name>W5UKA9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16 18">Protein quaking-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">qkib</name>
        <name evidence="18" type="synonym">qki2</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="14">Homodimer; does not require RNA to homodimerize.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the quaking family.</text>
    </comment>
    <dbReference type="EMBL" id="JT417719">
        <property type="protein sequence ID" value="AHH42487.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053540865.1">
        <property type="nucleotide sequence ID" value="XM_053684890.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UKA9"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048024">
        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014866">
        <property type="term" value="P:skeletal myofibril assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22465">
        <property type="entry name" value="KH-I_Hqk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4010:FF:000001">
        <property type="entry name" value="protein quaking isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000055">
        <property type="entry name" value="protein quaking isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032377">
        <property type="entry name" value="STAR_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF152">
        <property type="entry name" value="PROTEIN QUAKING-B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16544">
        <property type="entry name" value="STAR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="K Homology" evidence="15">
        <location>
            <begin position="80"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00064982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AHH42487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_053540865.1"/>
        </source>
    </evidence>
    <sequence length="317" mass="35118" checksum="41ED872B9ADBF601" modified="2014-04-16" version="1">MVGEMETKEKPKPTPDYLMQLMNDKKLMSSLPNFCGIFNHLERLLDEEIGRVRKDMYNDTLNSSTDKRTSELPDAVGPSAQLQEKLYVPVKEYPDFNFVGRILGPRGLTAKQLEAETGCKIMVRGKGSMRDKKKEEQNRGKPNWEHLNEDLHVLITVEDSQNRAEIKLKRAVEEVKKLLVPAAEGEDNLKKMQLMELAILNGTYRDANIKSPALAFSLAAQAPRLITGPSPVMPTAALRTPAPTGPTLMPLIRQIQTSALMPAGTPHPTATLMPQTPESGIIYTPYEYPYTLAPATSILEYPIDSSGVLGMAFPTKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5UKE0</accession>
    <name>W5UKE0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NHERF family PDZ scaffold protein 4b</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="4">Na(+)/H(+) exchange regulatory cofactor NHE-RF3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">PDZK1</name>
        <name evidence="6" type="synonym">nherf4b</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT417845">
        <property type="protein sequence ID" value="AHH42522.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017315812.1">
        <property type="nucleotide sequence ID" value="XM_017460323.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UKE0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012766"/>
    <dbReference type="GeneID" id="108260234"/>
    <dbReference type="KEGG" id="ipu:108260234"/>
    <dbReference type="CTD" id="566852"/>
    <dbReference type="OMA" id="HEELGCR"/>
    <dbReference type="OrthoDB" id="10009200at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06768">
        <property type="entry name" value="PDZ_NHERF-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051067">
        <property type="entry name" value="NHER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14191:SF20">
        <property type="entry name" value="NA(+)_H(+) EXCHANGE REGULATORY COFACTOR NHE-RF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14191">
        <property type="entry name" value="PDZ DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ" evidence="3">
        <location>
            <begin position="34"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="3">
        <location>
            <begin position="145"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="3">
        <location>
            <begin position="254"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="3">
        <location>
            <begin position="397"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="356"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="489"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="364"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="496"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="507"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AHH42522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017315812.1"/>
        </source>
    </evidence>
    <sequence length="517" mass="57135" checksum="B93911276530421F" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5ULC8</accession>
    <name>W5ULC8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8 10">Fibroblast growth factor receptor substrate 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">FRS2</name>
        <name evidence="10" type="synonym">frs2b</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT414082">
        <property type="protein sequence ID" value="AHH40492.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053538115.1">
        <property type="nucleotide sequence ID" value="XM_053682140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ULC8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012103"/>
    <dbReference type="KEGG" id="ipu:108268588"/>
    <dbReference type="GeneTree" id="ENSGT00940000157033"/>
    <dbReference type="OMA" id="THNHRGQ"/>
    <dbReference type="OrthoDB" id="8817077at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005104">
        <property type="term" value="F:fibroblast growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005068">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008543">
        <property type="term" value="P:fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01202">
        <property type="entry name" value="PTB_FRS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050996">
        <property type="entry name" value="Docking_Protein_DOK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038742">
        <property type="entry name" value="FRS2_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002404">
        <property type="entry name" value="IRS_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21258">
        <property type="entry name" value="DOCKING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21258:SF40">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR RECEPTOR SUBSTRATE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02174">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01244">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00310">
        <property type="entry name" value="PTBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51064">
        <property type="entry name" value="IRS_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0519">Myristate</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IRS-type PTB" evidence="7">
        <location>
            <begin position="13"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="128"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="208"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="263"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="438"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="161"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="269"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="453"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AHH40492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053538115.1"/>
        </source>
    </evidence>
    <sequence length="514" mass="55946" checksum="C8704395380830D1" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5UM00</accession>
    <name>W5UM00_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PAK2</name>
        <name evidence="15" type="synonym">pak2b</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JT418482">
        <property type="protein sequence ID" value="AHH42750.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047013988.1">
        <property type="nucleotide sequence ID" value="XM_047158032.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UM00"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000037221"/>
    <dbReference type="KEGG" id="ipu:108271286"/>
    <dbReference type="GeneTree" id="ENSGT00950000182988"/>
    <dbReference type="OMA" id="FGCAQIT"/>
    <dbReference type="OrthoDB" id="2914378at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01093">
        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06655">
        <property type="entry name" value="STKc_PAK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000011">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000069">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000001">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035064">
        <property type="entry name" value="STK_PAK2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF21">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 13 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="12">
        <location>
            <begin position="77"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="256"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="146"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="223"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AHH42750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_047013988.1"/>
        </source>
    </evidence>
    <sequence length="531" mass="58797" checksum="9D0983C9FDD836FB" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5UMB4</accession>
    <name>W5UMB4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peroxisomal targeting signal 1 receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">PTS1-BP</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Peroxin-5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">Pex5</name>
        <name evidence="22" type="synonym">pex5</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">In addition to promoting peroxisomal translocation of proteins containing a PTS1 peroxisomal targeting signal, mediates peroxisomal import of proteins containing a C-terminal PTS2-type peroxisomal targeting signal via its interaction with PEX7. Interaction with PEX7 only takes place when PEX7 is associated with cargo proteins containing a PTS2 peroxisomal targeting signal. PEX7 along with PTS2-containing cargo proteins are then translocated through the PEX13-PEX14 docking complex together with PEX5.</text>
    </comment>
    <comment type="function">
        <text evidence="18">Receptor that mediates peroxisomal import of proteins containing a C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type). Binds to cargo proteins containing a PTS1 peroxisomal targeting signal in the cytosol, and translocates them into the peroxisome matrix by passing through the PEX13-PEX14 docking complex along with cargo proteins. PEX5 receptor is then retrotranslocated into the cytosol, leading to release of bound cargo in the peroxisome matrix, and reset for a subsequent peroxisome import cycle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxisomal targeting signal receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="JT414900">
        <property type="protein sequence ID" value="AHH40802.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017325620.1">
        <property type="nucleotide sequence ID" value="XM_017470131.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UMB4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009892"/>
    <dbReference type="GeneID" id="108266596"/>
    <dbReference type="KEGG" id="ipu:108266596"/>
    <dbReference type="CTD" id="5830"/>
    <dbReference type="GeneTree" id="ENSGT00940000156605"/>
    <dbReference type="OrthoDB" id="10006023at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005052">
        <property type="term" value="F:peroxisome matrix targeting signal-1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000034">
        <property type="entry name" value="Peroxisomal biogenesis factor 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024111">
        <property type="entry name" value="PEX5/PEX5L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10130:SF2">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 1 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10130">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 1 RECEPTOR PEX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="14" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0882">Thioester bond</keyword>
    <keyword evidence="9 19" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="13" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="repeat" description="TPR" evidence="19">
        <location>
            <begin position="477"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="19">
        <location>
            <begin position="511"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="19">
        <location>
            <begin position="545"/>
            <end position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00046072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00046106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="AHH40802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017325620.1"/>
        </source>
    </evidence>
    <sequence length="641" mass="71958" checksum="AAC003EA04F54D27" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5UMN5</accession>
    <name>W5UMN5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Heat shock protein beta-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Heat shock 27 kDa protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14 16" type="primary">hspb1</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="JT419201">
        <property type="protein sequence ID" value="AHH42990.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017316198.1">
        <property type="nucleotide sequence ID" value="XM_017460709.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UMN5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018290"/>
    <dbReference type="GeneID" id="108260422"/>
    <dbReference type="KEGG" id="ipu:108260422"/>
    <dbReference type="CTD" id="3315"/>
    <dbReference type="GeneTree" id="ENSGT00940000155882"/>
    <dbReference type="OMA" id="QWPSHTS"/>
    <dbReference type="OrthoDB" id="10060792at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014881">
        <property type="term" value="P:regulation of myofibril size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046685">
        <property type="term" value="P:response to arsenic-containing substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd06475">
        <property type="entry name" value="ACD_HspB1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000024">
        <property type="entry name" value="heat shock protein beta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037876">
        <property type="entry name" value="ACD_HspB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001436">
        <property type="entry name" value="Alpha-crystallin/sHSP_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640:SF7">
        <property type="entry name" value="HEAT SHOCK PROTEIN BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640">
        <property type="entry name" value="HEAT SHOCK PROTEIN HSP-12.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14 16" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="13">
        <location>
            <begin position="90"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AHH42990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017316198.1"/>
        </source>
    </evidence>
    <sequence length="211" mass="23425" checksum="EA4EBF0D728C716B" modified="2014-04-16" version="1">MTERRIPFTFLRTPSWDPFRDWYQGSRIFDQAFGMPAVPEEMHVFPSTHWPGYMRPSIGADMASLMHSAQVPPLTMPTPIVPHPAAYARALSRQLSTGLSEIKQTQDGWKVSLDVNHFAPEELTVKTKDGVVEITGKHEERKDEHGVISRSFTRKYTLPPGADAEKITSSLSPEGFLTVEAPLPKPAIQSSEITIPVNTSSSVVQKQDGAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5URY3</accession>
    <name>W5URY3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-binding cassette sub-family F member 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Abcf2-1</name>
        <name evidence="13 14 15" type="synonym">abcf2b</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2013" name="PLoS ONE" volume="8" first="E63895" last="E63895">
            <title>Genome-wide identification, characterization and phylogenetic analysis of 50 catfish ATP-binding cassette (ABC) transporter genes.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Li Q."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23696857"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13 14 15" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13 14 15">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="JT417384">
        <property type="protein sequence ID" value="AHH42363.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017328439.1">
        <property type="nucleotide sequence ID" value="XM_017472950.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017328442.1">
        <property type="nucleotide sequence ID" value="XM_017472953.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017328443.1">
        <property type="nucleotide sequence ID" value="XM_017472954.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5URY3"/>
    <dbReference type="GeneID" id="108268161"/>
    <dbReference type="KEGG" id="ipu:108268161"/>
    <dbReference type="CTD" id="100000576"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000467">
        <property type="entry name" value="ATP-binding cassette sub-family F member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000104">
        <property type="entry name" value="ATP-binding cassette sub-family F member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF15">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 11 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="80"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="390"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="156"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AHH42363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017328439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017328442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017328443.1"/>
        </source>
    </evidence>
    <sequence length="610" mass="69877" checksum="BE02CD00031F2418" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W6A3K9</accession>
    <name>W6A3K9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Protein unc-13 B</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="14">Protein unc-13 homolog B isoform X5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">UNC13B</name>
        <name evidence="14" type="synonym">unc13bb</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT407515">
        <property type="protein sequence ID" value="AHI50409.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JT455259">
        <property type="protein sequence ID" value="AHI50460.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053542918.1">
        <property type="nucleotide sequence ID" value="XM_053686943.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098831">
        <property type="term" value="C:presynaptic active zone cytoplasmic component"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043195">
        <property type="term" value="C:terminal bouton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019992">
        <property type="term" value="F:diacylglycerol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017075">
        <property type="term" value="F:syntaxin-1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061789">
        <property type="term" value="P:dense core granule priming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099525">
        <property type="term" value="P:presynaptic dense core vesicle exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035249">
        <property type="term" value="P:synaptic transmission, glutamatergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016081">
        <property type="term" value="P:synaptic vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016082">
        <property type="term" value="P:synaptic vesicle priming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20859">
        <property type="entry name" value="C1_Munc13-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08394">
        <property type="entry name" value="C2A_Munc13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04027">
        <property type="entry name" value="C2B_Munc13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08395">
        <property type="entry name" value="C2C_Munc13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.50:FF:000001">
        <property type="entry name" value="Protein unc-13 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000002">
        <property type="entry name" value="Protein unc-13 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000014">
        <property type="entry name" value="protein unc-13 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000031">
        <property type="entry name" value="Protein unc-13 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000001">
        <property type="entry name" value="Protein unc-13 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1100:FF:000002">
        <property type="entry name" value="Unc-13, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010439">
        <property type="entry name" value="MUN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014770">
        <property type="entry name" value="Munc13_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014772">
        <property type="entry name" value="Munc13_dom-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027080">
        <property type="entry name" value="Unc-13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037302">
        <property type="entry name" value="Unc-13_C2B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10480">
        <property type="entry name" value="PROTEIN UNC-13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10480:SF8">
        <property type="entry name" value="PROTEIN UNC-13 HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06292">
        <property type="entry name" value="MUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01145">
        <property type="entry name" value="DUF1041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51258">
        <property type="entry name" value="MHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51259">
        <property type="entry name" value="MHD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2" evidence="8">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="9">
        <location>
            <begin position="464"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="8">
        <location>
            <begin position="570"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="MHD1" evidence="10">
        <location>
            <begin position="1001"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="domain" description="MHD2" evidence="11">
        <location>
            <begin position="1249"/>
            <end position="1388"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="8">
        <location>
            <begin position="1402"/>
            <end position="1529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="176"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="291"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="184"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="234"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="246"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="317"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AHI50460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_053542918.1"/>
        </source>
    </evidence>
    <sequence length="1574" mass="178675" checksum="A16EE04CE6B86CFD" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PHM1</accession>
    <name>A0A2D0PHM1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6 7">Synaptonemal complex protein 3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6 7 8 9" type="primary">LOC108254925</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5 6 7" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5 6 7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the XLR/SYCP3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017305893.1">
        <property type="nucleotide sequence ID" value="XM_017450404.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017305895.1">
        <property type="nucleotide sequence ID" value="XM_017450406.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017305896.1">
        <property type="nucleotide sequence ID" value="XM_017450407.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017305897.1">
        <property type="nucleotide sequence ID" value="XM_017450408.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017305898.1">
        <property type="nucleotide sequence ID" value="XM_017450409.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PHM1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000024699"/>
    <dbReference type="GeneID" id="108254925"/>
    <dbReference type="KEGG" id="ipu:108254925"/>
    <dbReference type="GeneTree" id="ENSGT00390000000062"/>
    <dbReference type="OrthoDB" id="9621324at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000795">
        <property type="term" value="C:synaptonemal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007286">
        <property type="term" value="P:spermatid development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051443">
        <property type="entry name" value="XLR/SYCP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006888">
        <property type="entry name" value="XLR/SYCP3/FAM9_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19368">
        <property type="entry name" value="XLR/SCP3/FAM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19368:SF15">
        <property type="entry name" value="XLR_SYCP3_FAM9 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04803">
        <property type="entry name" value="Cor1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XLR/SYCP3/FAM9" evidence="3">
        <location>
            <begin position="53"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017305893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017305895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017305896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017305897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017305898.1"/>
        </source>
    </evidence>
    <sequence length="206" mass="23943" checksum="EBC29964B47CF87F" modified="2017-12-20" version="1">MSLKDCTSSRDTNNTGATVRRPKRRSSTDRSLTVSHGGEVKTMLDTFGAEINKSFVAKRKCLESFTSSSLKTSQQKIEALWRSHQRERAQACEDYSTRFSSVFQQWESDMQRNKDQDKKLTSLFQHQQIMFQQMRASQTERLKMLKEIVDHYIKSTQALQDAHEEQNIVAMSELRQETALLHKKILMNTQQEEMVSVRNALQSMLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PKN7</accession>
    <name>A0A2D0PKN7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Protein timeless homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">timeless</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the timeless family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017306586.1">
        <property type="nucleotide sequence ID" value="XM_017451097.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053530100.1">
        <property type="nucleotide sequence ID" value="XM_053674125.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PKN7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018630"/>
    <dbReference type="GeneID" id="108255266"/>
    <dbReference type="KEGG" id="ipu:108255266"/>
    <dbReference type="CTD" id="8914"/>
    <dbReference type="OMA" id="LTRNVAM"/>
    <dbReference type="OrthoDB" id="310853at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031298">
        <property type="term" value="C:replication fork protection complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043111">
        <property type="term" value="P:replication fork arrest"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044998">
        <property type="entry name" value="Timeless"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007725">
        <property type="entry name" value="TIMELESS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006906">
        <property type="entry name" value="Timeless_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22940:SF4">
        <property type="entry name" value="PROTEIN TIMELESS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22940">
        <property type="entry name" value="TIMEOUT/TIMELESS-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26019">
        <property type="entry name" value="HTH_TIMELESS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04821">
        <property type="entry name" value="TIMELESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05029">
        <property type="entry name" value="TIMELESS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Timeless N-terminal" evidence="6">
        <location>
            <begin position="20"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Timeless C-terminal" evidence="7">
        <location>
            <begin position="1014"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="523"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="935"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1110"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1155"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="523"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="959"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="970"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="987"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1158"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1170"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1217"/>
            <end position="1226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017306586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053530100.1"/>
        </source>
    </evidence>
    <sequence length="1245" mass="144107" checksum="2F0E629630A7A605" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PL01</accession>
    <name>A0A2D0PL01_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ABC-type oligopeptide transporter ABCB9</fullName>
            <ecNumber evidence="16">7.4.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">ATP-binding cassette sub-family B member 9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">ATP-binding cassette transporter 9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">TAP-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">abcb9</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">SDA103</strain>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">ATP-dependent low-affinity peptide transporter which translocates a broad spectrum of peptides from the cytosol to the lysosomal lumen for degradation. Displays a broad peptide length specificity from 6-mer up to at least 59-mer peptides with an optimum of 23-mers. Binds and transports smaller and larger peptides with the same affinity. Favors positively charged, aromatic or hydrophobic residues in the N- and C-terminal positions whereas negatively charged residues as well as asparagine and methionine are not favored.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a [oligopeptide](in) + ATP + H2O = a [oligopeptide](out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14429"/>
            <dbReference type="Rhea" id="RHEA-COMP:10531"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83228"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.4.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:14430"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer. Interacts (via TMD0 region) with LAMP1; this interaction strongly stabilizes ABCB9 and protects ABCB9 against lysosomal degradation. Interacts (via TMD0 region) with LAMP2 (isoform LAMP-2B). Interacts (via TMD0) with YIF1B; this interaction allows (but is not essential) the ER-to-Golgi trafficking and strongly depends on a salt bridge within TMD0.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. MHC peptide exporter (TC 3.A.1.209) subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.4.2.6" evidence="16"/>
    <dbReference type="RefSeq" id="XP_017306684.1">
        <property type="nucleotide sequence ID" value="XM_017451195.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PL01"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033282"/>
    <dbReference type="GeneID" id="108255310"/>
    <dbReference type="KEGG" id="ipu:108255310"/>
    <dbReference type="CTD" id="23457"/>
    <dbReference type="OMA" id="CRLYEPQ"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015440">
        <property type="term" value="F:ABC-type peptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18784">
        <property type="entry name" value="ABC_6TM_ABCB9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000031">
        <property type="entry name" value="ATP-binding cassette sub-family B member 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000140">
        <property type="entry name" value="Lipid A export ATP-binding/permease protein MsbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030254">
        <property type="entry name" value="ABCB9_6-TMD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF21">
        <property type="entry name" value="ATP BINDING CASSETTE SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002773">
        <property type="entry name" value="ABC_prm/ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="11 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="238"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="24">
        <location>
            <begin position="199"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="23">
        <location>
            <begin position="513"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="754"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="156"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00062472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00066336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00068474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00079330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00083142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00084061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_017306684.1"/>
        </source>
    </evidence>
    <sequence length="787" mass="86956" checksum="3EEBE71DE99F7EC7" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PLB4</accession>
    <name>A0A2D0PLB4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein FAM219A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">fam219ab</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM219 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017306784.1">
        <property type="nucleotide sequence ID" value="XM_017451295.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PLB4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032574"/>
    <dbReference type="GeneID" id="108255368"/>
    <dbReference type="KEGG" id="ipu:108255368"/>
    <dbReference type="CTD" id="553801"/>
    <dbReference type="OMA" id="TACWDEA"/>
    <dbReference type="OrthoDB" id="6119141at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029339">
        <property type="entry name" value="FAM219"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31281">
        <property type="entry name" value="PROTEIN FAM219A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31281:SF0">
        <property type="entry name" value="PROTEIN FAM219A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15260">
        <property type="entry name" value="FAM219A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="65"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017306784.1"/>
        </source>
    </evidence>
    <sequence length="184" mass="20290" checksum="7494811E2E518FB1" modified="2017-12-20" version="1">MMEEIDRFQVPTEAEMQALDPASSTASEGDSDTRESEPATLNYKPSPLQRKIEKQRELARKGSLKNGTAGSPVNQQPKKNNVMARTRLVVPNKGYSSLDQSPDEKPLVALDTDSDDDFDVSRYSSSGYSSAEQINQDLNIQLLKDGYRLDEIPDDEDLDLIPPKSVNPTCMCCQATSSSACQIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PNW7</accession>
    <name>A0A2D0PNW7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Nuclear receptor ROR-beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">rorb</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Nuclear receptor that binds DNA as a monomer to ROR response elements (RORE). Required for proper cerebellum development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the nuclear hormone receptor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017306770.1">
        <property type="nucleotide sequence ID" value="XM_017451281.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PNW7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000031922"/>
    <dbReference type="GeneID" id="108255355"/>
    <dbReference type="KEGG" id="ipu:108255355"/>
    <dbReference type="CTD" id="6096"/>
    <dbReference type="OrthoDB" id="8832025at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06968">
        <property type="entry name" value="NR_DBD_ROR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06939">
        <property type="entry name" value="NR_LBD_ROR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000005">
        <property type="entry name" value="Nuclear orphan receptor ROR-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000003">
        <property type="entry name" value="Nuclear orphan receptor ROR-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044101">
        <property type="entry name" value="NR_DBD_ROR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003079">
        <property type="entry name" value="ROR_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45805">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR HR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45805:SF6">
        <property type="entry name" value="NUCLEAR RECEPTOR ROR-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01293">
        <property type="entry name" value="RORNUCRECPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9 12 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="14">
        <location>
            <begin position="7"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="15">
        <location>
            <begin position="207"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="91"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017306770.1"/>
        </source>
    </evidence>
    <sequence length="456" mass="51665" checksum="99B9D7BE3CBD6CFF" modified="2017-12-20" version="1">MRAQIEVIPCKICGDKSSGIHYGVITCEGCKGFFRRSQQNNASYSCPRQRNCLIDRTNRNRCQHCRLQKCLALGMSRDAVKFGRMSKKQRDSLYAEVQKHQQRLQEQRQQQTGDVYSSSLSTLGHDLGGAYTNGHVIDMAKTHANGAPAAYYSMDSTQPSPDQSGLDMAGMKPIKQEPVYDLTPVPDLFPYNSYQDSQLAPGVSMGELDRIAQNIIKSHLETCQYTGEELQQLSWQTHSYEEVKMYQSKSREALWQQCAIQITHAIQYVVEFAKRIAGFMELCQNDQILLLKSGCLEVVLVRMCRAFNPLNNTVLFEGKYGSIQMFKALGCDDLVTAMFDFAKSLCSLQLTEEEIALFSAAVLISTDRPWLMEPRKVQKLQEKIYLALQHIMQKNHMDEDALTKLIGRIPTLAALCTLHTEELQAFQQLHPETVNMLFPPLYKELFNPDAASVMSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PPZ6</accession>
    <name>A0A2D0PPZ6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Coiled-coil domain-containing protein 158 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC108256072</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017308072.1">
        <property type="nucleotide sequence ID" value="XM_017452583.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PPZ6"/>
    <dbReference type="GeneID" id="108256072"/>
    <dbReference type="KEGG" id="ipu:108256072"/>
    <dbReference type="OrthoDB" id="10072099at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031809">
        <property type="entry name" value="CCDC158"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47615">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 158"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47615:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 158"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15921">
        <property type="entry name" value="CCDC158"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="861"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="156"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="310"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="455"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="968"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="861"/>
            <end position="872"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017308072.1"/>
        </source>
    </evidence>
    <sequence length="1031" mass="118848" checksum="5A424AFBB47409A9" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PS12</accession>
    <name>A0A2D0PS12_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Merlin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Moesin-ezrin-radixin-like protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Neurofibromin-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Schwannomin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21 22" type="primary">nf2a</name>
    </gene>
    <organism evidence="20 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21 22" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21 22">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017307869.1">
        <property type="nucleotide sequence ID" value="XM_017452380.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017307870.1">
        <property type="nucleotide sequence ID" value="XM_017452381.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PS12"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000028920"/>
    <dbReference type="GeneID" id="108255967"/>
    <dbReference type="KEGG" id="ipu:108255967"/>
    <dbReference type="CTD" id="561184"/>
    <dbReference type="OMA" id="PGMLANE"/>
    <dbReference type="OrthoDB" id="6018897at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13194">
        <property type="entry name" value="FERM_C_ERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000103">
        <property type="entry name" value="Merlin isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000003">
        <property type="entry name" value="Radixin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000002">
        <property type="entry name" value="radixin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.450:FF:000001">
        <property type="entry name" value="radixin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011174">
        <property type="entry name" value="ERM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011259">
        <property type="entry name" value="ERM_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041789">
        <property type="entry name" value="ERM_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046810">
        <property type="entry name" value="ERM_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008954">
        <property type="entry name" value="Moesin_tail_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23281">
        <property type="entry name" value="MERLIN/MOESIN/EZRIN/RADIXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00769">
        <property type="entry name" value="ERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20492">
        <property type="entry name" value="ERM_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002305">
        <property type="entry name" value="ERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00661">
        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48678">
        <property type="entry name" value="Moesin tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
        <property type="entry name" value="FERM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="FERM" evidence="19">
        <location>
            <begin position="22"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="320"/>
            <end position="345"/>
        </location>
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        <location>
            <begin position="515"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="76"/>
            <end position="79"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)</name>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)</name>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00079831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00081139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR002305-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017307869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017307870.1"/>
        </source>
    </evidence>
    <sequence length="591" mass="69403" checksum="42F48EE168A840D5" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PS46</accession>
    <name>A0A2D0PS46_ICTPU</name>
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        <submittedName>
            <fullName evidence="8">Carcinoembryonic antigen-related cell adhesion molecule 6</fullName>
        </submittedName>
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        <name evidence="8" type="primary">vsig10l</name>
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    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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            <taxon>Ostariophysi</taxon>
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            <taxon>Ictalurus</taxon>
        </lineage>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017308854.2">
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    <dbReference type="GeneID" id="108256460"/>
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    <dbReference type="OrthoDB" id="6159398at2759"/>
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        <property type="component" value="Chromosome 23"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_sub"/>
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        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052598">
        <property type="entry name" value="IgSF_CEA-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44337:SF20">
        <property type="entry name" value="CARCINOEMBRYONIC ANTIGEN-RELATED CELL ADHESION MOLECULE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44337">
        <property type="entry name" value="CARCINOEMBRYONIC ANTIGEN-RELATED CELL ADHESION MOLECULE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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    </dbReference>
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        <source>
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    </evidence>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017308854.2"/>
        </source>
    </evidence>
    <sequence length="411" mass="43861" checksum="474D365D4EEE7FB5" modified="2023-02-22" version="1">MCESMEDMMFYFAITGTDCLLFIWPNGPTLVNAFVGTNVTLGVSYSGVTEPLVTWLKGTFILATWTIGLNTPPPAIAPAYSNVLSIDQTGSLVFNKVPASYSGTYTVQMVKPGAHEASVNFTLSVYNIITAVSVVAVSQDVIEGGPPFTLSYSSMQGPVLTSMWYFKGSAVVNSSRYLITPKSLTINQPNHNDTGIYSVVLTNPFSNVTQSENITVLYGPDQPVLEVSPTKAAFVSGETVSLSCRAEGVPPPSASWVFNGQPLPSSNGTLQLAHVQASQSGVYKCVLVNSRTSMGITRNVTINVLGLSGSAIAGIAAGVPCAILLLLLFAGLVYLSFHCYKKKATQNLRYPIAGAVKKAVISQPELTKPQHLLTSGFKLPPPYNPHHHQAQSERSGWLPLGIPPVRMATTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PS58</accession>
    <name>A0A2D0PS58_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rapunzel 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpz4</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SDA103</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017308256.1">
        <property type="nucleotide sequence ID" value="XM_017452767.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PS58"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009203"/>
    <dbReference type="GeneID" id="108256180"/>
    <dbReference type="KEGG" id="ipu:108256180"/>
    <dbReference type="CTD" id="550609"/>
    <dbReference type="OMA" id="DREARTN"/>
    <dbReference type="OrthoDB" id="9939466at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039051">
        <property type="entry name" value="SE-CTX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40472:SF9">
        <property type="entry name" value="RAPUNZEL 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40472">
        <property type="entry name" value="RICIN B-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_017308256.1"/>
        </source>
    </evidence>
    <sequence length="391" mass="44712" checksum="98148DE6C69C4715" modified="2017-12-20" version="1">MAENLQKLVASKKDVVENVMEVFEQGAEMLASIAGDLFPVFSIAAPIVKMALDNVESKEAEYMKEQFQKVRERLEVISEEIQRINDEVRKSGMDAAYFSVEENITNQFRKYMDILNAKPKFREAKKKQFLEHFSKSGGDKNLHTLYGAVTGDSFSGESVLEITLNYEQKSRRAMEDFCARLKQLFCIGLIALMGHTALKGGDDEEELLRNWAENMKVVQSKMNVVIEDCINSFPSQAEIDIKRLVRNHKDKSNQQLADMIIENLKGKYDWVSWSVRVFNSPKGLFTSKKDFQCATGKSRFQVPSPDENLNVVLSYSASPEPLDKAQIQQLVQDQKKVTVPGIAELIFEKTPMCVVHAVKTSCKEMAYSWSFRDELHYFEEFKNVYLFVHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PTA0</accession>
    <name>A0A2D0PTA0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Sentrin-specific protease 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">SUMO-1-specific protease 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Sentrin/SUMO-specific protease SENP6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">senp6a</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. Processes preferentially poly-SUMO2 and poly-SUMO3 chains, but does not efficiently process SUMO1, SUMO2 and SUMO3 precursors. Deconjugates SUMO1 from RXRA, leading to transcriptional activation. Involved in chromosome alignment and spindle assembly, by regulating the kinetochore CENPH-CENPI-CENPK complex. Desumoylates PML and CENPI, protecting them from degradation by the ubiquitin ligase RNF4, which targets polysumoylated proteins for proteasomal degradation. Also desumoylates RPA1, thus preventing recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombination.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with RXRA. Forms a complex with KAT5-TIP60 and UBE2I in response to UV irradiation. Interacts with RPA1 to maintain it in hyposumoylated state during S phase preventing DNA repair initiation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017308651.2">
        <property type="nucleotide sequence ID" value="XM_017453162.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PTA0"/>
    <dbReference type="GeneID" id="108256370"/>
    <dbReference type="KEGG" id="ipu:108256370"/>
    <dbReference type="CTD" id="503939"/>
    <dbReference type="OrthoDB" id="442460at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070139">
        <property type="term" value="F:SUMO-specific endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090234">
        <property type="term" value="P:regulation of kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090169">
        <property type="term" value="P:regulation of spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.130:FF:000001">
        <property type="entry name" value="sentrin-specific protease 6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.20:FF:000005">
        <property type="entry name" value="sentrin-specific protease 6 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.20:FF:000010">
        <property type="entry name" value="sentrin-specific protease 6 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.130:FF:000004">
        <property type="entry name" value="sentrin-specific protease 6 isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.130">
        <property type="entry name" value="Ubiquitin-related"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051947">
        <property type="entry name" value="Sentrin-specific_protease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46896">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46896:SF1">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6 20" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="18">
        <location>
            <begin position="710"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="326"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="828"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="931"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="250"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="349"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="833"/>
            <end position="846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00058874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00062458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00079020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017308651.2"/>
        </source>
    </evidence>
    <sequence length="1121" mass="124739" checksum="5F8C4FF2D63ACD06" modified="2023-02-22" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PU09</accession>
    <name>A0A2D0PU09_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sulfotransferase</fullName>
            <ecNumber evidence="15">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">hs3st2</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="15"/>
    <dbReference type="RefSeq" id="XP_017309519.1">
        <property type="nucleotide sequence ID" value="XM_017454030.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PU09"/>
    <dbReference type="GeneID" id="108256804"/>
    <dbReference type="KEGG" id="ipu:108256804"/>
    <dbReference type="CTD" id="9956"/>
    <dbReference type="OMA" id="FCRSTIM"/>
    <dbReference type="OrthoDB" id="411451at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008467">
        <property type="term" value="F:[heparan sulfate]-glucosamine 3-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000194">
        <property type="entry name" value="Sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037359">
        <property type="entry name" value="NST/OST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605:SF10">
        <property type="entry name" value="HEPARAN SULFATE GLUCOSAMINE 3-O-SULFOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605">
        <property type="entry name" value="HEPARAN SULFATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="18">
        <location>
            <begin position="140"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="97"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="active site" description="For sulfotransferase activity" evidence="12">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="148"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="354"/>
            <end position="358"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="337"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017309519.1"/>
        </source>
    </evidence>
    <sequence length="391" mass="44403" checksum="BE16715AA57D6E1C" modified="2017-12-20" version="1">MANRVLSSRAAPSSSSKFSSRFMFIFTLSLSFTYVCYSVLFCSAPVMPSSRFEERRCARGDSANAKKLLQRSAACAEARNSSRSELKATAAPLRLAPNRSELSGTRSSRARGADTARSKPANVSTVSASAQRYGNKKLPNALIVGVKKGGTRAVLEFIRIHPDVRALGTEPHFFDRNYDKGMDWYRGLMPRTLDSQITMEKTPSYYVTREAPRRIASMSQDTKLIVVVRNPVTRAISDYTQTLSKKPDIPSFEELAFKNKSQGLVDTSWNAIRIGMYILHLENWLQYFRISQIHFVSGERLITDPAGELGRVQDFLGLKRIITDKHFYFNRTKGFPCLKKPESSSTPRCLGKSKGRTHVQIDQEVIEQLRDFYRPFNVKFYEMVGHDFRWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q7P9</accession>
    <name>A0A2D0Q7P9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="2">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC108259605</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ADP-ribosyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017314713.2">
        <property type="nucleotide sequence ID" value="XM_017459224.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q7P9"/>
    <dbReference type="GeneID" id="108259605"/>
    <dbReference type="KEGG" id="ipu:108259605"/>
    <dbReference type="OrthoDB" id="10028716at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016849">
        <property type="term" value="F:phosphorus-oxygen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030890">
        <property type="term" value="P:positive regulation of B cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.82.10">
        <property type="entry name" value="ADP Ribosyl Cyclase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003193">
        <property type="entry name" value="ADP-ribosyl_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10912">
        <property type="entry name" value="ADP-RIBOSYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10912:SF9">
        <property type="entry name" value="ADP-RIBOSYL CYCLASE_CYCLIC ADP-RIBOSE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02267">
        <property type="entry name" value="Rib_hydrolayse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52309">
        <property type="entry name" value="N-(deoxy)ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="17"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017314713.2"/>
        </source>
    </evidence>
    <sequence length="286" mass="31396" checksum="DAD105CA79891D22" modified="2023-06-28" version="2">MEDGKVRDHSYTKRRKLFLLIGVAVLVLVAVPAIVLGVSAAGKNSASSSDSFKNVVITRCETYLKENGNMSGKNDCEIIWRAFEQAYIGKDPCDVPPDAYDPLINSVKQDVACNTMLFWSKTKTMVHAFTDNRDCLVTLEDTLLGFLFDELTWCSKNESKETFTTDCPRCQNNPVRSFWIRASANFAANVSCGNVSAMLNGSLEAPFSSTSVFGSVEVKNLDPDKVAGLTVLLVTKDTDTTTCNHSSLNMLQTTLDTKIAYNCREVPYSTVEGCISDPEIPCSDCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q8D7</accession>
    <name>A0A2D0Q8D7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Protein BEAN1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">bean1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3 4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017313981.1">
        <property type="nucleotide sequence ID" value="XM_017458492.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017313982.1">
        <property type="nucleotide sequence ID" value="XM_017458493.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q8D7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020915"/>
    <dbReference type="GeneID" id="108259225"/>
    <dbReference type="KEGG" id="ipu:108259225"/>
    <dbReference type="CTD" id="146227"/>
    <dbReference type="OMA" id="EFPPACD"/>
    <dbReference type="OrthoDB" id="9085892at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039352">
        <property type="entry name" value="BEAN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36464">
        <property type="entry name" value="PROTEIN BEAN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36464:SF1">
        <property type="entry name" value="PROTEIN BEAN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017313981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017313982.1"/>
        </source>
    </evidence>
    <sequence length="197" mass="21569" checksum="968198B4E0A1A4C4" modified="2017-12-20" version="1">MIVAVTMCVFVSPLVVAGIVIGLVLFLSLIIIIIGSMRKRKHSHLNTDAADGFSFGGSTGELRSDCVDEFPPAFDFDSYTETIPIPQISAGYPDPPPRYDECVGRDATQIFTPTDDPPPYSHTSHTYPCTEGAELPEGTSWWSAHAHAGSIHPIRFQDVSPVFVLSLDELPPYEAVMEQQNRTIPLIANDDVKHTAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q9M0</accession>
    <name>A0A2D0Q9M0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="17 18 19">Zinc finger protein 280C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17 18 19 20" type="primary">znf280d</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17 18 19" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17 18 19">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May function as a transcription factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017314074.1">
        <property type="nucleotide sequence ID" value="XM_017458585.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017314075.1">
        <property type="nucleotide sequence ID" value="XM_017458586.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047007618.1">
        <property type="nucleotide sequence ID" value="XM_047151662.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053532539.1">
        <property type="nucleotide sequence ID" value="XM_053676564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q9M0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034213"/>
    <dbReference type="GeneID" id="108259268"/>
    <dbReference type="KEGG" id="ipu:108259268"/>
    <dbReference type="CTD" id="54816"/>
    <dbReference type="OMA" id="GDLMSKH"/>
    <dbReference type="OrthoDB" id="10032537at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000298">
        <property type="entry name" value="zinc finger protein 280D isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050527">
        <property type="entry name" value="Snail/Krueppel_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059074">
        <property type="entry name" value="zf-C2H2_Z280C_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057618">
        <property type="entry name" value="Znf_POGZ/Z280C-D-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388:SF34">
        <property type="entry name" value="ZINC FINGER PROTEIN 280D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25414">
        <property type="entry name" value="zf-C2H2_Z280C_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25429">
        <property type="entry name" value="zf-POGZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="460"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="205"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="589"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="736"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="799"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="217"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="595"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="762"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="810"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="897"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="912"/>
            <end position="924"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017314074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017314075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_047007618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_053532539.1"/>
        </source>
    </evidence>
    <sequence length="930" mass="103796" checksum="B94DC81D8AFEA943" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QBZ5</accession>
    <name>A0A2D0QBZ5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Threonine synthase-like 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">thnsl2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the threonine synthase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017316203.1">
        <property type="nucleotide sequence ID" value="XM_017460714.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QBZ5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022489"/>
    <dbReference type="GeneID" id="108260425"/>
    <dbReference type="KEGG" id="ipu:108260425"/>
    <dbReference type="CTD" id="55258"/>
    <dbReference type="OMA" id="NFERYLY"/>
    <dbReference type="OrthoDB" id="5203861at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046360">
        <property type="term" value="P:2-oxobutyrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009071">
        <property type="term" value="P:serine family amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01560">
        <property type="entry name" value="Thr-synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1380.10:FF:000003">
        <property type="entry name" value="THR4p Threonine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000047">
        <property type="entry name" value="Threonine synthase like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1380.10">
        <property type="entry name" value="Threonine synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029144">
        <property type="entry name" value="Thr_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037158">
        <property type="entry name" value="Thr_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051166">
        <property type="entry name" value="Threonine_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42690">
        <property type="entry name" value="THREONINE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42690:SF1">
        <property type="entry name" value="THREONINE SYNTHASE-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24857">
        <property type="entry name" value="THR4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14821">
        <property type="entry name" value="Thr_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Threonine synthase N-terminal" evidence="8">
        <location>
            <begin position="2"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="7">
        <location>
            <begin position="103"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604450-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017316203.1"/>
        </source>
    </evidence>
    <sequence length="485" mass="54033" checksum="D50EEEDC2321B15E" modified="2017-12-20" version="1">MQYCSTRGGVHGWDFQNVLFSGYAPDGGMFMPETIPSISPATLRSWCSLSYQQLVCEVCSLFIPEQLIPKHDLEALVSSALSKFSVPDAVKMVRLKGSLSILELFHGKTLAFKDLAMTCTTRFLEYFLRKDNRRAIILVGTSGDTGGSAISSVCDLSAVDVVVVFPRGRISQVQERQMTTHIEDNVHVFAADGSSDEIDVPLRKLFADQDLVNRYGLMSLNSVNWSRILVQLAHFIYAYLQLSGVRDTDRDRLPALEVVVPTGGAGNITAGCIVKHMGVPLHLVAMVNSNDVVHRAVQSGDFSMADCVKQTLAPAIDIQDPYNMERVFWLLSGKDGALVKKMMEQFQDTHTLSIPETLHKKLCSVMCSGSVSDEGIMEAIKRCWEENQYLVCPHTAVGVWHHYHCPVRNGENRCCIATASPAKFLEVVQKAGLTESIDVPEAVRQLETKETRYKHLERNENWERALRERIEAIGSARQQGAQFYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QDX5</accession>
    <name>A0A2D0QDX5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">UPF0184 protein C9orf16 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bbln</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017315926.1">
        <property type="nucleotide sequence ID" value="XM_017460437.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QDX5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000028425"/>
    <dbReference type="GeneID" id="108260290"/>
    <dbReference type="KEGG" id="ipu:108260290"/>
    <dbReference type="CTD" id="79095"/>
    <dbReference type="OMA" id="INLMLDQ"/>
    <dbReference type="OrthoDB" id="10050612at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005374">
        <property type="entry name" value="BBLN_eukaryota"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34344:SF1">
        <property type="entry name" value="BUBLIN COILED-COIL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34344">
        <property type="entry name" value="UPF0184 PROTEIN C9ORF16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03670">
        <property type="entry name" value="UPF0184"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="37"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017315926.1"/>
        </source>
    </evidence>
    <sequence length="91" mass="10382" checksum="F439CB8AB954B8AC" modified="2017-12-20" version="1">MKMSGPNGDPEIRADEGLLEDEEDFNEQEYAAINSMLDQINSCLDNLEERNDALNGKLHELLESNRQARQEFRAQLNETQPVEQKHPPSEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QER2</accession>
    <name>A0A2D0QER2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Sodium/potassium/calcium exchanger 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC108260433</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Ca(2+)(out) + K(+)(out) + 4 Na(+)(in) = Ca(2+)(in) + K(+)(in) + 4 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:69967"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC24A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017316216.1">
        <property type="nucleotide sequence ID" value="XM_017460727.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QER2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021274"/>
    <dbReference type="GeneID" id="108260433"/>
    <dbReference type="KEGG" id="ipu:108260433"/>
    <dbReference type="OMA" id="DATVVWW"/>
    <dbReference type="OrthoDB" id="2127281at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008273">
        <property type="term" value="F:calcium, potassium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000005">
        <property type="entry name" value="sodium/potassium/calcium exchanger 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000038">
        <property type="entry name" value="Solute carrier family 24 member 6a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004481">
        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00367">
        <property type="entry name" value="calcium/sodium antiporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="SODIUM/POTASSIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846:SF28">
        <property type="entry name" value="SODIUM_POTASSIUM_CALCIUM EXCHANGER 3-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="221"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="446"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="481"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="516"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="554"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="581"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="10">
        <location>
            <begin position="98"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="10">
        <location>
            <begin position="447"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017316216.1"/>
        </source>
    </evidence>
    <sequence length="602" mass="66910" checksum="14329E64C1FFB2C6" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QFD4</accession>
    <name>A0A2D0QFD4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Zinc finger protein 281</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">znf281a</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Transcription repressor that plays a role in regulation of embryonic stem cells (ESCs) differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs. Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G-rich box in the enhancer region of these genes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017317009.1">
        <property type="nucleotide sequence ID" value="XM_017461520.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QFD4"/>
    <dbReference type="GeneID" id="108260862"/>
    <dbReference type="KEGG" id="ipu:108260862"/>
    <dbReference type="CTD" id="100005963"/>
    <dbReference type="OMA" id="HYAQKPD"/>
    <dbReference type="OrthoDB" id="9899552at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000067">
        <property type="entry name" value="Vascular endothelial zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000042">
        <property type="entry name" value="Zinc finger protein 148"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000313">
        <property type="entry name" value="Zinc finger protein 281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF155">
        <property type="entry name" value="EARLY GROWTH RESPONSE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="193"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="221"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="249"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="277"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="133"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="320"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="637"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="355"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="377"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="681"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00067216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017317009.1"/>
        </source>
    </evidence>
    <sequence length="812" mass="87210" checksum="ABEBB1E8F61B4F05" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QG16</accession>
    <name>A0A2D0QG16_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Ankyrin repeat and protein kinase domain-containing protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">ripk3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017317249.1">
        <property type="nucleotide sequence ID" value="XM_017461760.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017317250.1">
        <property type="nucleotide sequence ID" value="XM_017461761.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QG16"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000014818"/>
    <dbReference type="GeneID" id="108260981"/>
    <dbReference type="KEGG" id="ipu:108260981"/>
    <dbReference type="CTD" id="11035"/>
    <dbReference type="OMA" id="CTTVMEE"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004706">
        <property type="term" value="F:JUN kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025735">
        <property type="entry name" value="RHIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF297">
        <property type="entry name" value="RECEPTOR-INTERACTING SERINE_THREONINE-PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12721">
        <property type="entry name" value="RHIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="17"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="424"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="434"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017317249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017317250.1"/>
        </source>
    </evidence>
    <sequence length="452" mass="50079" checksum="83D1BA742A2131AF" modified="2017-12-20" version="1">MDLLSCPTPPVVHDDCLESWQQIGAGGFGQIHRAKHKKWGMDVAIKILHDNDGSNSYLLREADLMRHGGNPNVLRILGVYKSQAQGDRSAIQMGLVMEYMERGSLADLQMALNGSLPWALTFRIINQIALGMNFLHQLEPPLLHLDLKPSNVLLDDSLNAKLTDFGLSKIAKNTSKVIEEEDEAVAGTTSYMPPEAFRSSQYVPTFSSDVYSYGILLWSVITGKQPYIIKFSSLVRFRVREGDRPDLTSLDSTQADGLGDLIDLMKRCWDSDPEKRPSFMDCIEVTKNVYELHKQHVPDAVHSVLKQLDNKDKNSSSFSSLCAHPQTENGLKEHTDPTVKTHIPTQEMGPIPTASGAGTFVQSRSPILPGMYCKPATSAPPINCRPLNTLRQNSVPSTVSICLSNVKGVQIGNNNYMNVNIQGTRQRQRHPTAPSGTRQPSKRQQNPPNIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QI45</accession>
    <name>A0A2D0QI45_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Neutral cholesterol ester hydrolase 1</fullName>
            <ecNumber evidence="16">3.1.1.71</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Acetylalkylglycerol acetylhydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Arylacetamide deacetylase-like 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">LOC108261838</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">SDA103</strain>
        </source>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="28">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>1-O-hexadecyl-2-acetyl-sn-glycerol + H2O = 1-O-hexadecyl-sn-glycerol + acetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38563"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:34115"/>
            <dbReference type="ChEBI" id="CHEBI:75936"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:38564"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>a 1-O-alkyl-2-acetyl-sn-glycerol + H2O = a 1-O-alkyl-sn-glycerol + acetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11552"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15850"/>
            <dbReference type="ChEBI" id="CHEBI:16291"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="EC" id="3.1.1.71"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:11553"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a cholesterol ester + H2O = cholesterol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36403"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:17002"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:36404"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>cholesteryl (9Z-octadecenoate) + H2O = cholesterol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33875"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:46898"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:33876"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Microsome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.71" evidence="16"/>
    <dbReference type="RefSeq" id="XP_017318098.1">
        <property type="nucleotide sequence ID" value="XM_017462609.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QI45"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013804"/>
    <dbReference type="GeneID" id="108261838"/>
    <dbReference type="KEGG" id="ipu:108261838"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047378">
        <property type="term" value="F:acetylalkylglycerol acetylhydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017157">
        <property type="entry name" value="Arylacetamide_deacetylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050300">
        <property type="entry name" value="GDXG_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033140">
        <property type="entry name" value="Lipase_GDXG_put_SER_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081">
        <property type="entry name" value="AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48081:SF29">
        <property type="entry name" value="NEUTRAL CHOLESTEROL ESTER HYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037251">
        <property type="entry name" value="Arylacetamide_deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01174">
        <property type="entry name" value="LIPASE_GDXG_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 28" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="25" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="25">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012745356" description="Neutral cholesterol ester hydrolase 1" evidence="25">
        <location>
            <begin position="19"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="26">
        <location>
            <begin position="113"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="26">
        <location>
            <begin position="325"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="active site" evidence="23 24">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <feature type="active site" evidence="23">
        <location>
            <position position="353"/>
        </location>
    </feature>
    <feature type="active site" evidence="23">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00049214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR037251-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_017318098.1"/>
        </source>
    </evidence>
    <sequence length="413" mass="46223" checksum="55326712D2F36A31" modified="2017-12-20" version="1">MMIRMRLLLFSSVLLALALSYFRVPALPGTVAEPCKLLLLDAVFRSMMWAAKLVQDLGLSNQMCVLNYMALFAELRAPRSSESVRVTDTSFSGVQARVFESMAPGSRRLKRGVVYFHGGGWALGSARMRSYDLLCRMMAEELDAVVISVDYRLAPEVYFPGQYEDAIQACRSILTDEVLARFSVDPHRLAVSGDSAGGNLAAAVAQELASDSSVSIRFKVQALIYPVLQALDFNTPSYQQNGDVPILYRSLMVCFWLEYLNGDQNLVPALLVNNHTALDQNRDVASAREKLNWTRLLAPKFTKNYKPVVPHHGSPEILQQLPGLLDTRASPLLAEPEVLKLVPPAYIMTCEHDVLRDDGLMYARRLEEAGVPVTSDHYEQGFHGIMMFGFFPASFSVGWQSQRNYLHWLQHNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QIA6</accession>
    <name>A0A2D0QIA6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">C-C motif chemokine 4-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC108261919</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017318153.2">
        <property type="nucleotide sequence ID" value="XM_017462664.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QIA6"/>
    <dbReference type="GeneID" id="108261919"/>
    <dbReference type="KEGG" id="ipu:108261919"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00272">
        <property type="entry name" value="Chemokine_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039809">
        <property type="entry name" value="Chemokine_b/g/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015">
        <property type="entry name" value="SMALL INDUCIBLE CYTOKINE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00199">
        <property type="entry name" value="SCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0202">Cytokine</keyword>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
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        <location>
            <begin position="22"/>
            <end position="99"/>
        </location>
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        <location>
            <begin position="29"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017318153.2"/>
        </source>
    </evidence>
    <sequence length="99" mass="11112" checksum="D9C2C57E3B653A33" modified="2023-06-28" version="2">MRNLTALLFLLLLCSLQLVSSAPAGTDFRITCCAKTTKVKIPLKSIVTYWWTINDCPIKAIVFETLNKKQHCVDPTAVWVNHHMKAVDLRNSTTITAKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QLR6</accession>
    <name>A0A2D0QLR6_ICTPU</name>
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        <submittedName>
            <fullName evidence="5">Kelch-like protein 10</fullName>
        </submittedName>
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        <name evidence="5" type="primary">LOC108263297</name>
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    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017319448.3">
        <property type="nucleotide sequence ID" value="XM_017463959.3"/>
    </dbReference>
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    <dbReference type="STRING" id="7998.ENSIPUP00000014605"/>
    <dbReference type="GeneID" id="108263297"/>
    <dbReference type="KEGG" id="ipu:108263297"/>
    <dbReference type="OMA" id="KIRLAWM"/>
    <dbReference type="OrthoDB" id="191037at2759"/>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
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        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
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    </dbReference>
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        <property type="entry name" value="BACK"/>
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        <property type="entry name" value="BTB-kelch_protein"/>
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        <property type="entry name" value="BTB/POZ_dom"/>
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        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
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    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
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    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF172">
        <property type="entry name" value="KELCH-LIKE PROTEIN 10"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="21"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017319448.3"/>
        </source>
    </evidence>
    <sequence length="566" mass="65176" checksum="CC015FDDB4EEF223" modified="2023-06-28" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QR97</accession>
    <name>A0A2D0QR97_ICTPU</name>
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        <submittedName>
            <fullName evidence="8">Interleukin-12 subunit beta</fullName>
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        <name evidence="8" type="primary">si:dkey-88e18.2</name>
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        <name type="scientific">Ictalurus punctatus</name>
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        <lineage>
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            <taxon>Actinopterygii</taxon>
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        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053533766.1">
        <property type="nucleotide sequence ID" value="XM_053677791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QR97"/>
    <dbReference type="GeneID" id="108264170"/>
    <dbReference type="KEGG" id="ipu:108264170"/>
    <dbReference type="CTD" id="100001799"/>
    <dbReference type="OrthoDB" id="9945899at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050676">
        <property type="entry name" value="IL-12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48485:SF3">
        <property type="entry name" value="INTERLEUKIN-12 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48485">
        <property type="entry name" value="INTERLEUKIN-12 SUBUNIT BETA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043399973" evidence="6">
        <location>
            <begin position="21"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="295"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="297"/>
            <end position="322"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053533766.1"/>
        </source>
    </evidence>
    <sequence length="322" mass="36850" checksum="59C42C725E463001" modified="2017-12-20" version="1">MRASLSIITVLCLCSLKVSALDTFPEKFVAGLNGSSVTLSCDKAIKTPVTWNFEDEIVESHEDMFELEGNTLRLPKLEVDVIGNFTCWSEGQEVDYTYVLMDMSDTITDSPVSCTAENFNCTSTISCTMKEKEYQYFRLRDERNDGWLTPSTDGRFLLTHKSNPFAEEAEPIVLIGEAVSNLKYYFKIHYSFYIRDIIRPGCPRVSVLKKNNLSVLDVNPPDSWTLPLSFFPLQHEIEFQKRTDGKIISQLYPEPNNTDSSIGSITIPTGTSKLRARCRDSLLLSQWSEWTVWQNVGKRRPKNGKNPKKKDKKKKRIHHKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QSU7</accession>
    <name>A0A2D0QSU7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquitin-like protein 7b isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ubl7b</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
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            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017321369.1">
        <property type="nucleotide sequence ID" value="XM_017465880.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QSU7"/>
    <dbReference type="GeneID" id="108264398"/>
    <dbReference type="CTD" id="393331"/>
    <dbReference type="OrthoDB" id="10016665at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14326">
        <property type="entry name" value="UBA_UBL7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000041">
        <property type="entry name" value="ubiquitin-like protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000139">
        <property type="entry name" value="ubiquitin-like protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015496">
        <property type="entry name" value="Ubiquilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047878">
        <property type="entry name" value="UBL7_UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10677">
        <property type="entry name" value="UBIQUILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10677:SF25">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="287"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017321369.1"/>
        </source>
    </evidence>
    <sequence length="368" mass="39213" checksum="6FBA3F2454E3D36E" modified="2017-12-20" version="1">MENTFWHLSLKLHDRPKSVLPFPEDVEPGRYMVATIKQLVSAQLPDSLPDPELIELVHCGCKLKDDLTLDAYGIKSGSTLHILKKVWPEPEVKPEPVNRSAAAREFRLLQATMHSNIAYRDAVFKMLSNKESLDQIIVASPGLSSDPVALGVLQDKDLFVQFTDPNLLDVLVRSHPALVNAIILILHSVTSSMPGQSNASSSHNTPASSYSEMPGGFLFEGMSDDEDDFQSGSRAGSSQRPRPPTNLSGGGATGPRPITQSELATALALANTPESSAATPILGSQDVSTGSSSAAAGTPVTSSVLSRALQQALEASGVTPLQGRWQTQMQQLRDMGIRDEDLALRALQATDGDLQAALELIFAGGGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QT21</accession>
    <name>A0A2D0QT21_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Extracellular calcium-sensing receptor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC108263927</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017320640.3">
        <property type="nucleotide sequence ID" value="XM_017465151.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QT21"/>
    <dbReference type="GeneID" id="108263927"/>
    <dbReference type="KEGG" id="ipu:108263927"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000016">
        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF528">
        <property type="entry name" value="C-FAMILY ODORANT RECEPTOR OLFCD2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="544"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="582"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="614"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="658"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="703"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="738"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="770"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="544"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017320640.3"/>
        </source>
    </evidence>
    <sequence length="813" mass="90764" checksum="2A05B25B9AFC23C6" modified="2023-06-28" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QY36</accession>
    <name>A0A2D0QY36_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="3">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ube2d4</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017323312.1">
        <property type="nucleotide sequence ID" value="XM_017467823.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QY36"/>
    <dbReference type="SMR" id="A0A2D0QY36"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005278"/>
    <dbReference type="GeneID" id="108265473"/>
    <dbReference type="KEGG" id="ipu:108265473"/>
    <dbReference type="CTD" id="51619"/>
    <dbReference type="OMA" id="MEMAVTH"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23792">
        <property type="entry name" value="UBCc_UBE2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000101">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="10">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="8">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017323312.1"/>
        </source>
    </evidence>
    <sequence length="147" mass="16663" checksum="EF90A2C6BC677254" modified="2017-12-20" version="1">MALKRIQKELTDLQRDPPAQCSAGPVGEDLFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPEIAHTYKADREKYNRLAREWTQKYAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QZQ1</accession>
    <name>A0A2D0QZQ1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16 17 18">Mitofusin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16 17 18" type="primary">mfn2</name>
    </gene>
    <organism evidence="15 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16 17 18" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16 17 18">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017323865.1">
        <property type="nucleotide sequence ID" value="XM_017468376.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017323866.1">
        <property type="nucleotide sequence ID" value="XM_017468377.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047011420.1">
        <property type="nucleotide sequence ID" value="XM_047155464.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QZQ1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030433"/>
    <dbReference type="GeneID" id="108265739"/>
    <dbReference type="KEGG" id="ipu:108265739"/>
    <dbReference type="CTD" id="9927"/>
    <dbReference type="OMA" id="YRINCES"/>
    <dbReference type="OrthoDB" id="6256226at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008053">
        <property type="term" value="P:mitochondrial fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051646">
        <property type="term" value="P:mitochondrion localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09912">
        <property type="entry name" value="DLP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000012">
        <property type="entry name" value="Mitofusin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000214">
        <property type="entry name" value="Mitofusin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006884">
        <property type="entry name" value="Fzo/mitofusin_HR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027094">
        <property type="entry name" value="Mitofusin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10465:SF1">
        <property type="entry name" value="MITOFUSIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10465">
        <property type="entry name" value="TRANSMEMBRANE GTPASE FZO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04799">
        <property type="entry name" value="Fzo_mitofusin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111479">
        <property type="entry name" value="Fzo-like conserved region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="629"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="Dynamin-type G" evidence="14">
        <location>
            <begin position="93"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="700"/>
            <end position="737"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017323865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017323866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_047011420.1"/>
        </source>
    </evidence>
    <sequence length="757" mass="85898" checksum="696507D4E1D2682D" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R0Z9</accession>
    <name>A0A2D0R0Z9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Host cell factor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">C1 factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22 23" type="primary">hcfc1b</name>
    </gene>
    <organism evidence="21 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
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    </reference>
    <reference evidence="22 23" key="2">
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            <authorList>
                <consortium name="RefSeq"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22 23">Blood</tissue>
        </source>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="host cell factor 1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Kelch-type beta propeller"/>
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        <property type="entry name" value="HCF1/2"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Kelch_HCF"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="HOST CELL FACTOR 1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="18" id="KW-0175">Coiled coil</keyword>
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        <location>
            <begin position="1610"/>
            <end position="1700"/>
        </location>
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            <end position="1817"/>
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            <end position="437"/>
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            <begin position="1101"/>
            <end position="1118"/>
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            <begin position="1128"/>
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        <location>
            <begin position="1140"/>
            <end position="1153"/>
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            <begin position="1175"/>
            <end position="1184"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00074287"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00081526"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50853"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017323700.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017323701.2"/>
        </source>
    </evidence>
    <sequence length="1846" mass="189130" checksum="E87B85F04A578551" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R550</accession>
    <name>A0A2D0R550_ICTPU</name>
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        <submittedName>
            <fullName evidence="24 25">Metalloreductase STEAP3</fullName>
        </submittedName>
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        <name evidence="24 25" type="primary">steap3</name>
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    <organism evidence="23 25">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
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    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
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    <reference evidence="24 25" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24 25">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>2 Cu(+) + NADP(+) + H(+) = 2 Cu(2+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:71771"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
            <dbReference type="ChEBI" id="CHEBI:49552"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
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        <physiologicalReaction direction="right-to-left" evidence="18">
            <dbReference type="Rhea" id="RHEA:71773"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>2 Fe(2+) + NADP(+) + H(+) = 2 Fe(3+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:71767"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="19">
            <dbReference type="Rhea" id="RHEA:71769"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the STEAP family.</text>
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    <dbReference type="GeneTree" id="ENSGT00390000008042"/>
    <dbReference type="OMA" id="WESKRNC"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
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    <dbReference type="GO" id="GO:0010008">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008823">
        <property type="term" value="F:cupric reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000051">
        <property type="entry name" value="STEAP2 metalloreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051267">
        <property type="entry name" value="STEAP_metalloreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14239">
        <property type="entry name" value="DUDULIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14239:SF8">
        <property type="entry name" value="METALLOREDUCTASE STEAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0410">Iron transport</keyword>
    <keyword evidence="17 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="302"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="357"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="396"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="429"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="22">
        <location>
            <begin position="28"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Ferric oxidoreductase" evidence="21">
        <location>
            <begin position="259"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF01794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_017325698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_017325699.1"/>
        </source>
    </evidence>
    <sequence length="486" mass="53901" checksum="F8EC763D1038021B" modified="2017-12-20" version="1">MSNEMHVPLLLDELDTVDAVHVTPASSTVAILGSGDFSRSLAMRLVACGFGVVVGSRCVRRISPGLFLDAVELNSQEAAVAKAKSLVFMALFPEYYPSLLGLRSALAGKILVDVSNAVAMNSKRPSNAEQLAEMFPDSVVVKGFNTISAWSLQTGAQDGNKQVLLCSDSAEAKHEVGKLARRMGFYPVDVGGLCCAQNLERKPLHLFPSWRGPVLTTFLLFFFFYCYGFIRGILLPYLAYGYNSFYRLVLDLVNESLPCVALVSLALVYLPGLFAAWLQLWRGTKYQRFPRWLDAWLLRRKQLGLLSFLCAALHGVYSLCLPLRTITRRRLINAAFSQVKSGVEMPWDEQDVWRTELYVSSGVLGLGILSLLAITSLPSVGNILTWREFTFIQSGLGYAALTLSVMHTLVLGWNFAFSPQSYAFYMPPSYMLAVALPCAVLVCRCFLLLPCVSAKLARIRRGWESTRKCPVSQHHRVQANRTVPQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R7I0</accession>
    <name>A0A2D0R7I0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein aqp1a.2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">aqp1a.2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017326544.2">
        <property type="nucleotide sequence ID" value="XM_017471055.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R7I0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007755"/>
    <dbReference type="GeneID" id="108267090"/>
    <dbReference type="KEGG" id="ipu:108267090"/>
    <dbReference type="CTD" id="559284"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035379">
        <property type="term" value="F:carbon dioxide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015168">
        <property type="term" value="F:glycerol transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006972">
        <property type="term" value="P:hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003097">
        <property type="term" value="P:renal water transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139">
        <property type="entry name" value="AQUAPORIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139:SF161">
        <property type="entry name" value="AQUAPORIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="9"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="304"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="343"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="385"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="426"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="458"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="500"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017326544.2"/>
        </source>
    </evidence>
    <sequence length="560" mass="59209" checksum="93EECE2F67444DE1" modified="2023-02-22" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RA68</accession>
    <name>A0A2D0RA68_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Tax1-binding protein 1 homolog B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">tax1bp1b</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May have anti-apoptotic activity.</text>
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    <dbReference type="RefSeq" id="XP_017326996.1">
        <property type="nucleotide sequence ID" value="XM_017471507.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RA68"/>
    <dbReference type="GeneID" id="108267440"/>
    <dbReference type="KEGG" id="ipu:108267440"/>
    <dbReference type="CTD" id="324152"/>
    <dbReference type="OrthoDB" id="10015001at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21969">
        <property type="entry name" value="Zn-C2H2_TAX1BP1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21970">
        <property type="entry name" value="Zn-C2H2_TAX1BP1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2840:FF:000002">
        <property type="entry name" value="Tax1-binding protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012852">
        <property type="entry name" value="CALCOCO1-like"/>
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    <dbReference type="InterPro" id="IPR041641">
        <property type="entry name" value="CALCOCO1/2_Zn_UBZ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041611">
        <property type="entry name" value="SKICH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051002">
        <property type="entry name" value="UBA_autophagy_assoc_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31915">
        <property type="entry name" value="SKICH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31915:SF8">
        <property type="entry name" value="TAX1-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07888">
        <property type="entry name" value="CALCOCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17751">
        <property type="entry name" value="SKICH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18112">
        <property type="entry name" value="Zn-C2H2_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51905">
        <property type="entry name" value="ZF_UBZ1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBZ1-type" evidence="11">
        <location>
            <begin position="753"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="domain" description="UBZ1-type" evidence="11">
        <location>
            <begin position="780"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="329"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="472"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="602"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="662"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="147"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="343"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="602"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="693"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017326996.1"/>
        </source>
    </evidence>
    <sequence length="815" mass="93341" checksum="7211F74DBD907ED1" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RBT4</accession>
    <name>A0A2D0RBT4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase</fullName>
            <ecNumber evidence="15">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">plcb3</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + H2O = 1D-myo-inositol 1-phosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58433"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:43485"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:33180"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="17">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="15"/>
    <dbReference type="RefSeq" id="XP_017327335.1">
        <property type="nucleotide sequence ID" value="XM_017471846.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RBT4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000002329"/>
    <dbReference type="GeneID" id="108267602"/>
    <dbReference type="KEGG" id="ipu:108267602"/>
    <dbReference type="CTD" id="5331"/>
    <dbReference type="OMA" id="IWSEELF"/>
    <dbReference type="OrthoDB" id="269822at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120548">
        <property type="term" value="F:phosphatidylinositol phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13361">
        <property type="entry name" value="PH_PLC_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08591">
        <property type="entry name" value="PI-PLCc_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000048">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000008">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1230.10">
        <property type="entry name" value="Phospholipase C beta, distal C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016280">
        <property type="entry name" value="PLC-beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014815">
        <property type="entry name" value="PLC-beta_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042531">
        <property type="entry name" value="PLC-beta_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037862">
        <property type="entry name" value="PLC-beta_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053945">
        <property type="entry name" value="PLCB1-4-like_EFh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF11">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE BETA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17787">
        <property type="entry name" value="PH_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08703">
        <property type="entry name" value="PLC-beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22631">
        <property type="entry name" value="PLCB1-4-like_EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000956">
        <property type="entry name" value="PLC-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
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        <property type="entry name" value="PLCYc"/>
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        <property type="entry name" value="C-terminal domain of PLC-beta"/>
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    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
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    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 17" id="KW-0106">Calcium</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 15 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 15 18" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="9 15 18" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PI-PLC Y-box" evidence="22">
        <location>
            <begin position="570"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="21">
        <location>
            <begin position="689"/>
            <end position="813"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="472"/>
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    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1195"/>
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        <location>
            <begin position="930"/>
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    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="1004"/>
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    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="1064"/>
            <end position="1148"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="516"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="537"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="548"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1195"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="1224"/>
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    <feature type="active site" evidence="16">
        <location>
            <position position="334"/>
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    <feature type="active site" evidence="16">
        <location>
            <position position="381"/>
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    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="335"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="364"/>
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        <ligand>
            <name>Ca(2+)</name>
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        </ligand>
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    <feature type="binding site" evidence="17">
        <location>
            <position position="366"/>
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        <ligand>
            <name>Ca(2+)</name>
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    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
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    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023674"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023726"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000956-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000956-2"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_017327335.1"/>
        </source>
    </evidence>
    <sequence length="1246" mass="142489" checksum="BC350EA3E42C5185" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RC03</accession>
    <name>A0A2D0RC03_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="29 30">Non-lysosomal glucosylceramidase</fullName>
            <shortName evidence="30">NLGase</shortName>
            <ecNumber evidence="30">3.2.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="34" type="primary">gba2</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="33">SDA103</strain>
        </source>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="34">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="30">Non-lysosomal glucosylceramidase that catalyzes the hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>1-(beta-D-galactosyl)-N-dodecanoylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + N-dodecanoylsphing-4-enine</text>
            <dbReference type="Rhea" id="RHEA:70255"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:72956"/>
            <dbReference type="ChEBI" id="CHEBI:73432"/>
            <dbReference type="ChEBI" id="CHEBI:189066"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:70256"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="21">
            <dbReference type="Rhea" id="RHEA:70257"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a beta-D-galactosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-galactose</text>
            <dbReference type="Rhea" id="RHEA:14297"/>
            <dbReference type="ChEBI" id="CHEBI:4139"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18390"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="EC" id="3.2.1.46"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:14298"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>a beta-D-galactosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine + cholesterol = cholesteryl 3-beta-D-galactoside + an N-acylsphing-4-enine</text>
            <dbReference type="Rhea" id="RHEA:70235"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:18390"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:189066"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:70236"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="22">
            <dbReference type="Rhea" id="RHEA:70237"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine + H2O = an N-acylsphing-4-enine + D-glucose</text>
            <dbReference type="Rhea" id="RHEA:13269"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:22801"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="EC" id="3.2.1.45"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:13270"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate + chenodeoxycholate = beta-D-glucosyl-(1-&gt;3)-O-chenodeoxycholate + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:59104"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36234"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:142610"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:59105"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>beta-D-glucosyl-(1-&gt;3)-O-chenodeoxycholate + H2O = chenodeoxycholate + D-glucose</text>
            <dbReference type="Rhea" id="RHEA:58340"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:36234"/>
            <dbReference type="ChEBI" id="CHEBI:142610"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:58341"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>beta-D-glucosyl-(1-&gt;3)-O-lithocholate + H2O = lithocholate + D-glucose</text>
            <dbReference type="Rhea" id="RHEA:58344"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29744"/>
            <dbReference type="ChEBI" id="CHEBI:142611"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:58345"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>beta-D-glucosyl-N-(9Z-octadecenoyl)-sphing-4E-enine + cholesterol = N-(9Z-octadecenoyl)-sphing-4-enine + cholesteryl 3-beta-D-glucoside</text>
            <dbReference type="Rhea" id="RHEA:58324"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:17495"/>
            <dbReference type="ChEBI" id="CHEBI:77996"/>
            <dbReference type="ChEBI" id="CHEBI:139140"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:58325"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="23">
            <dbReference type="Rhea" id="RHEA:58326"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>cholesteryl 3-beta-D-glucoside + H2O = cholesterol + D-glucose</text>
            <dbReference type="Rhea" id="RHEA:11956"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:17495"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:11957"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>octyl beta-D-glucose + chenodeoxycholate = beta-D-glucosyl-(1-&gt;3)-O-chenodeoxycholate + octan-1-ol</text>
            <dbReference type="Rhea" id="RHEA:59108"/>
            <dbReference type="ChEBI" id="CHEBI:16188"/>
            <dbReference type="ChEBI" id="CHEBI:36234"/>
            <dbReference type="ChEBI" id="CHEBI:41128"/>
            <dbReference type="ChEBI" id="CHEBI:142610"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:59109"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Steroid metabolism; cholesterol metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="28 30">Belongs to the non-lysosomal glucosylceramidase family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.45" evidence="30"/>
    <dbReference type="RefSeq" id="XP_017328072.1">
        <property type="nucleotide sequence ID" value="XM_017472583.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RC03"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000011522"/>
    <dbReference type="GeneID" id="108267970"/>
    <dbReference type="KEGG" id="ipu:108267970"/>
    <dbReference type="CTD" id="57704"/>
    <dbReference type="OMA" id="HDLGAPN"/>
    <dbReference type="OrthoDB" id="730489at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:glycosyltransferase activity"/>
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        <property type="term" value="F:steryl-beta-glucosidase activity"/>
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        <property type="entry name" value="Non-lysos_glucosylceramidase"/>
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        <property type="entry name" value="BILE ACID BETA-GLUCOSIDASE-RELATED"/>
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        <property type="entry name" value="NON-LYSOSOMAL GLUCOSYLCERAMIDASE"/>
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        <property type="entry name" value="DUF608"/>
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        <property type="entry name" value="Glyco_hydr_116N"/>
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        <property type="entry name" value="Six-hairpin glycosidases"/>
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    <keyword evidence="18 30" id="KW-0326">Glycosidase</keyword>
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        <location>
            <begin position="95"/>
            <end position="399"/>
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            <end position="834"/>
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        <source>
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            <dbReference type="ARBA" id="ARBA00022676"/>
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        <source>
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        <source>
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        <source>
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            <dbReference type="ARBA" id="ARBA00051669"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00051786"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00060895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00074567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="PIRNR" id="PIRNR028944"/>
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        <source>
            <dbReference type="Pfam" id="PF04685"/>
        </source>
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        <source>
            <dbReference type="Pfam" id="PF12215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="RefSeq" id="XP_017328072.1"/>
        </source>
    </evidence>
    <sequence length="871" mass="98672" checksum="6450FB22A44DE65D" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RDJ5</accession>
    <name>A0A2D0RDJ5_ICTPU</name>
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        <recommendedName>
            <fullName evidence="9">Homeobox protein HB9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">mnx1</name>
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    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
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    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017473123.3"/>
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    <dbReference type="OMA" id="DERTHMS"/>
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        <property type="component" value="Chromosome 7"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0031018">
        <property type="term" value="P:endocrine pancreas development"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0048812">
        <property type="term" value="P:neuron projection morphogenesis"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021520">
        <property type="term" value="P:spinal cord motor neuron cell fate specification"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000243">
        <property type="entry name" value="Motor neuron and pancreas homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
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        <property type="entry name" value="HD_metazoa"/>
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    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
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    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
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    <dbReference type="InterPro" id="IPR042768">
        <property type="entry name" value="MNX1/Ceh-12"/>
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    <dbReference type="PANTHER" id="PTHR24335">
        <property type="entry name" value="MOTOR NEURON AND PANCREAS HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24335:SF3">
        <property type="entry name" value="MOTOR NEURON AND PANCREAS HOMEOBOX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="139"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="141"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="20"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="199"/>
            <end position="272"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="23"/>
            <end position="50"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="202"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="241"/>
            <end position="250"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="262"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00079148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017328612.1"/>
        </source>
    </evidence>
    <sequence length="272" mass="30419" checksum="1E0B509F9D40C1E7" modified="2017-12-20" version="1">MEKSKNFRIDAILAVEPPKAQTSPLALVTSLSSSSSTSSSSSGRRSVPGSDLLNSPSAMVGLHPPSSALYHPHMFSYPLGQPPVLSYPYPPLPHHEEPLKLPPGSLQLDHWLRISTAGLALPKLQPDFNPQAHQSTLLGKCRRPRTAFTSQQLLELEHQFKLNKYLSRPKRFEVATSLMLTETQVKIWFQNRRMKWKRSKKAKEQEAAQEAAEKHKGTKGKEKSEQPDSAKGSGRLKDFRDSDDDDDEADSYMCNSSECSSEDERTHMSPQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0REE4</accession>
    <name>A0A2D0REE4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13 14">Zinc finger protein 84</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13 14" type="primary">LOC108268204</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13 14">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017328511.1">
        <property type="nucleotide sequence ID" value="XM_017473022.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017328520.1">
        <property type="nucleotide sequence ID" value="XM_017473031.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0REE4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003532"/>
    <dbReference type="GeneID" id="108268204"/>
    <dbReference type="KEGG" id="ipu:108268204"/>
    <dbReference type="GeneTree" id="ENSGT00940000166158"/>
    <dbReference type="OMA" id="SVQPYQH"/>
    <dbReference type="OrthoDB" id="6910977at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000761">
        <property type="entry name" value="Zinc finger protein 449"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24408:SF58">
        <property type="entry name" value="TRANSCRIPTION FACTOR (TFIIIA), PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05150)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24408">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02178">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00384">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="122"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="150"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="316"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="345"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="373"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="168"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="196"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="211"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="246"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="262"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="294"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017328511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017328520.1"/>
        </source>
    </evidence>
    <sequence length="408" mass="45615" checksum="282BA4357B6D0C18" modified="2017-12-20" version="1">MDSDILKIEEIVMGGGETAAPADVNAEKAGKPYTAIIIQDPPPAPVLQSYQHESLQCFQCFITFCNSKAKERHMKKSHREEYKQQLQQCDTLFTCYVCDRTFPSSEELTLHQSTHNKEDKPFKCAHCGESFRTFSELTTHRRQVCYGRQFLCRECNETFRSQALLRTHRLAQHPARPDGDDTDNPSKTHRCGKCGRGFEEESELLSHQENHAANQHCNGSSPVKRRGRPPKAETSLATGEKKQKRSKNEENEIAEKSEDAPAAPPAPPAPPAVEAKPKTVGRRGRPPKSASQDPKSDEDKSDSKKTKAASPTARQIPCPECDLFFSAPAQLRAHKKEKHTQRKAHPCQECEESFNRAEQLEAHMARAHKAGRYSCSTCGKSFGRESNLKAHQQSHGKEDEKSAGGGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RES8</accession>
    <name>A0A2D0RES8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Centromere protein F isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">si:dkeyp-115e12.6</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017329042.3">
        <property type="nucleotide sequence ID" value="XM_017473553.3"/>
    </dbReference>
    <dbReference type="GeneID" id="108268529"/>
    <dbReference type="KEGG" id="ipu:108268529"/>
    <dbReference type="OrthoDB" id="10255522at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051310">
        <property type="term" value="P:metaphase chromosome alignment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010389">
        <property type="term" value="P:regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043513">
        <property type="entry name" value="Cenp-F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018302">
        <property type="entry name" value="CenpF/LEK1_Rb-prot-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018463">
        <property type="entry name" value="Centromere_CenpF_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18874:SF10">
        <property type="entry name" value="CENTROMERE PROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18874">
        <property type="entry name" value="CMF/LEK/CENP CELL DIVISION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10490">
        <property type="entry name" value="CENP-F_C_Rb_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10481">
        <property type="entry name" value="CENP-F_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Centromere protein Cenp-F N-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Kinetochore protein Cenp-F/LEK1 Rb protein-binding" evidence="4">
        <location>
            <begin position="1712"/>
            <end position="1745"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="179"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="343"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="518"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="689"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="913"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1459"/>
            <end position="1481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1509"/>
            <end position="1593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1625"/>
            <end position="1675"/>
        </location>
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        <location>
            <begin position="41"/>
            <end position="75"/>
        </location>
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        <location>
            <begin position="104"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="958"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1200"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1319"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="180"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="343"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="353"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="518"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="539"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="557"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="625"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="638"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="698"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="711"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="732"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1466"/>
            <end position="1476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1509"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1532"/>
            <end position="1552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1648"/>
            <end position="1663"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017329042.3"/>
        </source>
    </evidence>
    <sequence length="1771" mass="200561" checksum="E73279DFD285591E" modified="2023-06-28" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RGU9</accession>
    <name>A0A2D0RGU9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carbonic anhydrase</fullName>
            <ecNumber evidence="4">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ca5a</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017329758.1">
        <property type="nucleotide sequence ID" value="XM_017474269.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RGU9"/>
    <dbReference type="GeneID" id="108268921"/>
    <dbReference type="CTD" id="763"/>
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    <dbReference type="OrthoDB" id="429145at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018338">
        <property type="entry name" value="Carbonic_anhydrase_a-class_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF25">
        <property type="entry name" value="CARBONIC ANHYDRASE 5B, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00162">
        <property type="entry name" value="ALPHA_CA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="5">
        <location>
            <begin position="8"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017329758.1"/>
        </source>
    </evidence>
    <sequence length="278" mass="32034" checksum="C1ADF49CBB16FC90" modified="2017-12-20" version="1">MLCHKACEAAEYIKKMHPMWQEPLEMPGGERQSPIDISLRKSVFDPNLKPFVTQYDPRTCQQIWNNGYSFLVEYDDTTDKSTLRGGPLEDKFRLCQFHFHWGENNTWGSEHTVDRHLYPAELHLVHWNADKYALFEEAIMEENGLAVIGVFLKVGKRHEGLQKLVDALPAVRHKDSVVEFTKFDPSCLLPESMNEYWTYAGSLTTPPLTEAVTWIIMKQAIEVSHEQLAVFRSLLFTSAEEEVQKSMVNNFRVQQPLKGRTVRSSFSPFLENVPSTDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RHR3</accession>
    <name>A0A2D0RHR3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Phospholipase A and acyltransferase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">LOC108269055</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8 9">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the H-rev107 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017330064.1">
        <property type="nucleotide sequence ID" value="XM_017474575.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047013173.1">
        <property type="nucleotide sequence ID" value="XM_047157217.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RHR3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003831"/>
    <dbReference type="GeneID" id="108269055"/>
    <dbReference type="KEGG" id="ipu:108269055"/>
    <dbReference type="OMA" id="PYCIFRG"/>
    <dbReference type="OrthoDB" id="421951at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016410">
        <property type="term" value="F:N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008970">
        <property type="term" value="F:phospholipase A1 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070292">
        <property type="term" value="P:N-acylphosphatidylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.10">
        <property type="entry name" value="endopeptidase domain like (from Nostoc punctiforme)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051496">
        <property type="entry name" value="H-rev107_PLA/AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007053">
        <property type="entry name" value="LRAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13943">
        <property type="entry name" value="HRAS-LIKE SUPPRESSOR - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13943:SF31">
        <property type="entry name" value="PHOSPHOLIPASE A AND ACYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04970">
        <property type="entry name" value="LRAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51934">
        <property type="entry name" value="LRAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="LRAT" evidence="6">
        <location>
            <begin position="15"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017330064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_047013173.1"/>
        </source>
    </evidence>
    <sequence length="171" mass="18815" checksum="87238FAD9C4C920E" modified="2017-12-20" version="1">MAPGQFEEKPQPGDLIEIFRGTYQHWGIYVGDGYIIHLAPPTESAQAGAYSMMSVLCDKAVVKKEQLWDVAGTDKYTINNLLDKKYEPRPIRVILQEAHSLLGQGLPYCVLSGNCEHFVTELRYGKRESRQVRKAVEVGVAVSVGTLIGVGILAAASAFFGSSNKEKNNKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RIY0</accession>
    <name>A0A2D0RIY0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Protein NDRG4 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">ndrg4</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6 7">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NDRG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017330477.2">
        <property type="nucleotide sequence ID" value="XM_017474988.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053538125.1">
        <property type="nucleotide sequence ID" value="XM_053682150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RIY0"/>
    <dbReference type="GeneID" id="108269244"/>
    <dbReference type="KEGG" id="ipu:108269244"/>
    <dbReference type="CTD" id="65009"/>
    <dbReference type="OrthoDB" id="191979at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000009">
        <property type="entry name" value="NDRG family member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004142">
        <property type="entry name" value="NDRG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11034">
        <property type="entry name" value="N-MYC DOWNSTREAM REGULATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03096">
        <property type="entry name" value="Ndr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="402"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="434"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017330477.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053538125.1"/>
        </source>
    </evidence>
    <sequence length="456" mass="50114" checksum="A3248306D5DBD69C" modified="2023-06-28" version="2">MGPGLFWNFPSKTSCTVSQTLLQTMLLSKMAGMKEQQFIEEKPLLPETRGQDTEMARGACVNAGPCPQPARPTRKWHEIARHWLGVTRRHRTSGGCILENCESFMPVGDWKEHDIETPYGMLHVVIRGAPKGNKPAILTYHDVGLNHKLCFNTLFHNEDMQEITKHFVVCHIDAPGQQVGAPQMPAGYQYPTMDNLAGMLPSVVEHFGFKSIVGIGVGAGAYILAKFALIFPDLVEGLVLLNIDPNGKGWIDWAASKLSGLTSALPDTVLPHLFSQEELMNNSELVQSYRQQINNTINQFNLQLFWNMYNSRRDLEMNRSGTVLNAKTLRCPVMLVVGDNAPAEEGVVECNSKLDPTNTTFLKMADSGGLPQITQPGKLTEAFKYFLQGMGYIASIKDRRMSGGPVPSASMTRLARSRTASLTSGGSVDGSRNRACTHSDSTEGVGQLSHTMEVSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RN69</accession>
    <name>A0A2D0RN69_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">creatine kinase</fullName>
            <ecNumber evidence="2">2.7.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC108269944</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>creatine + ATP = N-phosphocreatine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17157"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57947"/>
            <dbReference type="ChEBI" id="CHEBI:58092"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.3.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:17158"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 10">Belongs to the ATP:guanido phosphotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.3.2" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017331606.1">
        <property type="nucleotide sequence ID" value="XM_017476117.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RN69"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000001162"/>
    <dbReference type="GeneID" id="108269944"/>
    <dbReference type="KEGG" id="ipu:108269944"/>
    <dbReference type="OMA" id="MRICAFN"/>
    <dbReference type="OrthoDB" id="430219at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004111">
        <property type="term" value="F:creatine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046314">
        <property type="term" value="P:phosphocreatine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00716">
        <property type="entry name" value="creatine_kinase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000026">
        <property type="entry name" value="Creatine kinase B-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.135.10:FF:000001">
        <property type="entry name" value="Creatine kinase M-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.135.10">
        <property type="entry name" value="ATP:guanido phosphotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000749">
        <property type="entry name" value="ATP-guanido_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022415">
        <property type="entry name" value="ATP-guanido_PTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022414">
        <property type="entry name" value="ATP-guanido_PTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022413">
        <property type="entry name" value="ATP-guanido_PTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036802">
        <property type="entry name" value="ATP-guanido_PTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547">
        <property type="entry name" value="ARGININE OR CREATINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547:SF23">
        <property type="entry name" value="CREATINE KINASE B-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00217">
        <property type="entry name" value="ATP-gua_Ptrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02807">
        <property type="entry name" value="ATP-gua_PtransN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48034">
        <property type="entry name" value="Guanido kinase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00112">
        <property type="entry name" value="PHOSPHAGEN_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51510">
        <property type="entry name" value="PHOSPHAGEN_KINASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51509">
        <property type="entry name" value="PHOSPHAGEN_KINASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphagen kinase N-terminal" evidence="11">
        <location>
            <begin position="11"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphagen kinase C-terminal" evidence="12">
        <location>
            <begin position="125"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="128"/>
            <end position="132"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="292"/>
            <end position="296"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="320"/>
            <end position="325"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017331606.1"/>
        </source>
    </evidence>
    <sequence length="381" mass="42837" checksum="1976A2CBFCF9BD29" modified="2017-12-20" version="1">MPFGNTHNQLKMKYSSKAEYPDLTKHNNHMAKVLTPALYKRLRNKQTPSGFTLDDVIQTGVDNPGHPFIMTVGCVAGDEETYEVFKELLDPVIKDRHGGYKPTDKHKTDLNPANLKGGDDLDPNYVLSSRVRTGRSIRGFCLPPHCSRGERRIIEKLSVEALGALSGDLKGKYYALKNMSEVEQQQLIDDHFLFDKPVSPLLLASGMARDWPDGRGIWHNVNKTFLVWVNEEDHLRVISMQKGGNMKEVFTRFCTGLTKIEELFKSKGHEFMWNEHLGYVLTCPSNLGTGLRGGVHVKLPNLSKHKKFEEVLKRLRLQKRGTGGVDTAAVGGVFDISNADRLGFSEVELVQMVVDGVKLLVEMEKQLEKGQDIDDLIPVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RRU3</accession>
    <name>A0A2D0RRU3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">2-aminoethanethiol (Cysteamine) dioxygenase b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">adob</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017332680.1">
        <property type="nucleotide sequence ID" value="XM_017477191.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RRU3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035611"/>
    <dbReference type="GeneID" id="108270460"/>
    <dbReference type="KEGG" id="ipu:108270460"/>
    <dbReference type="CTD" id="406474"/>
    <dbReference type="OMA" id="HFWCGGE"/>
    <dbReference type="OrthoDB" id="271433at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20289">
        <property type="entry name" value="cupin_ADO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012864">
        <property type="entry name" value="PCO/ADO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22966">
        <property type="entry name" value="2-AMINOETHANETHIOL DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22966:SF61">
        <property type="entry name" value="2-AMINOETHANETHIOL DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07847">
        <property type="entry name" value="PCO_ADO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017332680.1"/>
        </source>
    </evidence>
    <sequence length="253" mass="28395" checksum="EB5AEF377378705C" modified="2017-12-20" version="1">MMPQDNMSSLIQRLARQALTTFRNRASEESKAFVENQSKLRSLLGEVRAADLKIVPRCVDSSCVSPQLLGPPVTYMHICETDSFSMGVFLLKHGSCIPLHDHPNMYGMLKVLYGKVRVSCYDRLDCSPDSANGRHFNPPLLPFQKSGMRLSVQRSVEEYTEDSPPCVLSPYRDNLHQIEAVDGPTAFLDILAPPYDPDDGRDCHYYKVLQQGLESAERTAGGQEQTELWLMEIPQPSEFWCGAEPYPGPKVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RX81</accession>
    <name>A0A2D0RX81_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Solute carrier family 22 member 17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">slc22a17</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017334565.1">
        <property type="nucleotide sequence ID" value="XM_017479076.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RX81"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000036993"/>
    <dbReference type="GeneID" id="108271489"/>
    <dbReference type="KEGG" id="ipu:108271489"/>
    <dbReference type="CTD" id="51310"/>
    <dbReference type="OMA" id="HHVIYSS"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="283"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="398"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="426"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="452"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="482"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="514"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="118"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="323"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="542"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="325"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017334565.1"/>
        </source>
    </evidence>
    <sequence length="576" mass="62288" checksum="7A84FFAC538E5CD3" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S103</accession>
    <name>A0A2D0S103_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13 14">Polyhomeotic-like protein 2b isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13 14" type="primary">phc2b</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
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    <reference evidence="13 14" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <tissue evidence="13 14">Blood</tissue>
        </source>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S146</accession>
    <name>A0A2D0S146_ICTPU</name>
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        <recommendedName>
            <fullName evidence="8">NADH dehydrogenase (ubiquinone) complex I, assembly factor 6</fullName>
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        <name type="scientific">Ictalurus punctatus</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9">SDA103</strain>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="10">Blood</tissue>
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    <comment type="function">
        <text evidence="7">Involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I) at early stages. May play a role in the biogenesis of complex I subunit MT-ND1.</text>
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        <property type="entry name" value="Squ/phyt_synthse"/>
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        <property type="entry name" value="NADH DEHYDROGENASE (UBIQUINONE) COMPLEX I, ASSEMBLY FACTOR 6"/>
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        <source>
            <dbReference type="RefSeq" id="XP_017336469.1"/>
        </source>
    </evidence>
    <sequence length="325" mass="37645" checksum="C90AAEC6348AE686" modified="2017-12-20" version="1">MAAGVRIKCGLLNPRSLFCSKIFPHIVGSQTRTEVMCLRLATHSASQHDQKYCIDLVRLRDYEGFVGSLLLPEAARRSSLALRAFNVELAQVKDSVSQKTIGLMRMQFWKTAVEDIYRDEPPVQPVSAELWRAVRKHKLTRRWLLRIIAERENDLEDRAYRNLQELEAYAENTQSSLLYLLLETLGVKDVHADHAASHIGKAQGIVTCLRATLYHSRRYRVYLPMDICMLHGTSQEDFIRGSREQNVRDVVYDIASQAHIHLQHARSFNKNVPDAALPAFLQTVAIDDYLERVRKVDFDVFHPSLQRRNPLMPIQLYFRSWKKKY</sequence>
</entry>
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    <accession>A0A2D0S297</accession>
    <name>A0A2D0S297_ICTPU</name>
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        <submittedName>
            <fullName evidence="6">Pejvakin</fullName>
        </submittedName>
    </protein>
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        <name evidence="6" type="primary">pjvk</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the gasdermin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017336878.1">
        <property type="nucleotide sequence ID" value="XM_017481389.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S297"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026871"/>
    <dbReference type="GeneID" id="108272727"/>
    <dbReference type="KEGG" id="ipu:108272727"/>
    <dbReference type="CTD" id="494513"/>
    <dbReference type="OrthoDB" id="9436533at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012501">
        <property type="term" value="P:programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040460">
        <property type="entry name" value="Gasdermin_pore"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042377">
        <property type="entry name" value="GSDME"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15207">
        <property type="entry name" value="NONSYNDROMIC HEARING IMPAIRMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15207:SF2">
        <property type="entry name" value="PEJVAKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04598">
        <property type="entry name" value="Gasdermin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gasdermin pore forming" evidence="4">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017336878.1"/>
        </source>
    </evidence>
    <sequence length="369" mass="41695" checksum="8C34EAAD9C48EC34" modified="2017-12-20" version="1">MFAAATKNFVKQVGDTGRLIPVPSLSEADRYQPLSLVTRKRRRYFWKKNKYGSTPFSLKDILVGEKEITAGVSSYQLLNYEDKSDVSLNGRLGNHLIHEVGVNISGSDSVAVKASFGVVTKHEVEVPTLLRELNSRKVDLDHCLIRQSKDSGRTVLCVVMESIRTTRQCSLTVHAGMHGATMRFQIDDIHNPRGRDKSIVIPAHTTIAFSIFDLYVRLDGRLDICVAPESVGGFEKEQMREQLGGFIGRFSVGRLRRFLSGIIYGNPFRADDRTFEELTHSDLYMDDVVDYYEKAASMTDLSTSYLREDKHTRVNLLNHNIPKGLCALCSKSHQRRETVYGCLECSSGGQKYVRLHAVPCFDLWHKSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S392</accession>
    <name>A0A2D0S392_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vesicle transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">sft2d2b</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">May be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the SFT2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017336906.1">
        <property type="nucleotide sequence ID" value="XM_017481417.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S392"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000031609"/>
    <dbReference type="GeneID" id="108272735"/>
    <dbReference type="KEGG" id="ipu:108272735"/>
    <dbReference type="CTD" id="553724"/>
    <dbReference type="OMA" id="GNIFCLC"/>
    <dbReference type="OrthoDB" id="73614at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011691">
        <property type="entry name" value="Vesicle_transpt_SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137:SF1">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN SFT2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017336906.1"/>
        </source>
    </evidence>
    <sequence length="161" mass="17809" checksum="F96B57AE01EA89B3" modified="2017-12-20" version="1">MDKLKKVLSGQDGNDDLNVLQSAGDVTTLGWGTRVKAFIACFVLGVVCSILGSCLLWVPKKGLILFAVFYTFGNIASLLSTVFLMGPLNQLKRMCDKTRALATAVMITCLVLTLCSAFWWQNKGLAVLFCILQFLALTWYSLSYIPFARDALIKMFSMCLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SBA8</accession>
    <name>A0A2D0SBA8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 20">Cholesterol side-chain cleavage enzyme, mitochondrial</fullName>
            <ecNumber evidence="4 20">1.14.15.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Cholesterol desmolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">cyp11a</name>
        <name evidence="22" type="synonym">cyp11a1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones. Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>6 reduced [adrenodoxin] + cholesterol + 3 O2 + 6 H(+) = 4-methylpentanal + pregnenolone + 6 oxidized [adrenodoxin] + 4 H2O</text>
            <dbReference type="Rhea" id="RHEA:35739"/>
            <dbReference type="Rhea" id="RHEA-COMP:9998"/>
            <dbReference type="Rhea" id="RHEA-COMP:9999"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:16581"/>
            <dbReference type="ChEBI" id="CHEBI:17998"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="EC" id="1.14.15.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 20">Lipid metabolism; C21-steroid hormone metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="20">Mitochondrion inner membrane</location>
            <topology evidence="20">Peripheral membrane protein</topology>
        </subcellularLocation>
        <text evidence="20">Localizes to the matrix side of the mitochondrion inner membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.15.6" evidence="4 20"/>
    <dbReference type="RefSeq" id="XP_017340018.1">
        <property type="nucleotide sequence ID" value="XM_017484529.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SBA8"/>
    <dbReference type="GeneID" id="100313513"/>
    <dbReference type="CTD" id="100313513"/>
    <dbReference type="OrthoDB" id="3945418at2759"/>
    <dbReference type="UniPathway" id="UPA00229"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008386">
        <property type="term" value="F:cholesterol monooxygenase (side-chain-cleaving) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006700">
        <property type="term" value="P:C21-steroid hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071375">
        <property type="term" value="P:cellular response to peptide hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034650">
        <property type="term" value="P:cortisol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006704">
        <property type="term" value="P:glucocorticoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050479">
        <property type="entry name" value="CYP11_CYP27_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279:SF3">
        <property type="entry name" value="CHOLESTEROL SIDE-CHAIN CLEAVAGE ENZYME, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 20" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="7 20" id="KW-0349">Heme</keyword>
    <keyword evidence="12 20" id="KW-0408">Iron</keyword>
    <keyword evidence="14 20" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15 20" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13 20" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="11 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18 20" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="19 20" id="KW-0755">Steroidogenesis</keyword>
    <keyword evidence="17 20" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="10 20" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU364077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017340018.1"/>
        </source>
    </evidence>
    <sequence length="466" mass="53146" checksum="FA514AD8E3056555" modified="2017-12-20" version="1">MGMGMGMGMGVMAVRCTTWNSSLARSLAMLEGPGQVRACHSGSMPVAKETLFPASSSTVKPFNEIPGMWKNSVGNLYTFWKLDGFKNIHHIMVHNFNTFGPIYREKIGYYESVNIINPEDAAILFKAEGHYPKRLRVEPWTSYRDFRNRKYGVLLKDGEDWRSNRVILNREVISPKVQGNFVPLLDEVGQDFVARVYKKIQRSGQNKWTTDLSHELFKYALESVSAVLYGERLGLLLDYIDPEAQHFIDCITLMFKTTCPMLYIPPSMLRRLGAKVWKDHVEAWDGIFNQADHCIQNIYRQLRKESGEQNKYPGVLASLLMLDRLSIEEIKASVTELMAGGVDTTSITLLWTLYELARHPDLQEELRAEIIAARAASQGDTVQMLKMVPLLKGALKETLRLHPVAVSLQRYLTQDVVIQNYHIPSGMLENFKIEKQRQLEVRSTFELILVPEKPILLTIKPLHSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SD69</accession>
    <name>A0A2D0SD69_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">T-cell leukemia homeobox protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC108274503</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017340195.1">
        <property type="nucleotide sequence ID" value="XM_017484706.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SD69"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000011800"/>
    <dbReference type="GeneID" id="108274503"/>
    <dbReference type="KEGG" id="ipu:108274503"/>
    <dbReference type="OMA" id="VHHEPIS"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000040">
        <property type="entry name" value="T-cell leukemia homeobox protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042247">
        <property type="entry name" value="TLX1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45921">
        <property type="entry name" value="IP01054P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45921:SF1">
        <property type="entry name" value="T-CELL LEUKEMIA HOMEOBOX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="9">
        <location>
            <begin position="147"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="149"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="37"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017340195.1"/>
        </source>
    </evidence>
    <sequence length="275" mass="30776" checksum="2A08AC7EB7A574A0" modified="2017-12-20" version="1">MESAQSPRPEQSSKPSHEPIRFGIDRILGSSDRQHGSESSTDSQTGTTTTFTALLAPLSAFPGSLEDPSEFVPNRTLGHRGVIRVPAHRPLGAAIAPSVVSAAPGFGPLCFPWVDNHRRFTKDRLPALLALPITRRIGHPYQNRTPPKRKKPRTSFSRVQICELEKRFHRQKYLASAERAALAKTLKMTDAQVKTWFQNRRTKWRRQTAEEREAERQQANRMLLQLQADALQKSISESVPVDPLCVHNSSLYALQNLQPWAQDKAGKMATTTSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SDS2</accession>
    <name>A0A2D0SDS2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ankyrin repeat domain-containing protein 26 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">si:ch211-272n13.3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_017485375.3"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
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        <property type="entry name" value="CC144C-like_CC_dom"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <location>
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        <location>
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        <location>
            <begin position="1243"/>
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        <location>
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        <location>
            <begin position="2217"/>
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        </location>
    </feature>
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        <location>
            <begin position="261"/>
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    <feature type="compositionally biased region" description="Gly residues" evidence="4">
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    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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    </feature>
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        <location>
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        <location>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF12001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14915"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017340864.1"/>
        </source>
    </evidence>
    <sequence length="2365" mass="268058" checksum="2529F76B8F54E182" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SE92</accession>
    <name>A0A2D0SE92_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 19">Polypeptide N-acetylgalactosaminyltransferase</fullName>
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        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">galnt14</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + UDP + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:12788"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:53604"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + UDP + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="ChEBI" id="CHEBI:87075"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 19">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 19">Golgi apparatus membrane</location>
            <topology evidence="2 19">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017485297.3"/>
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    <dbReference type="OrthoDB" id="429263at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
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    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000020">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF8">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 19" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13 19" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 19" id="KW-0430">Lectin</keyword>
    <keyword evidence="16 19" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B lectin" evidence="20">
        <location>
            <begin position="433"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00052209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017340786.1"/>
        </source>
    </evidence>
    <sequence length="562" mass="64675" checksum="FF08E26FF49E806B" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SGZ5</accession>
    <name>A0A2D0SGZ5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Photoreceptor ankyrin repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ankrd33aa</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017341988.1">
        <property type="nucleotide sequence ID" value="XM_017486499.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SGZ5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012358"/>
    <dbReference type="GeneID" id="108275591"/>
    <dbReference type="KEGG" id="ipu:108275591"/>
    <dbReference type="CTD" id="557568"/>
    <dbReference type="OrthoDB" id="10057496at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173">
        <property type="entry name" value="ANKYRIN REPEAT CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173:SF29">
        <property type="entry name" value="PHOTORECEPTOR ANKYRIN REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="159"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="394"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="420"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017341988.1"/>
        </source>
    </evidence>
    <sequence length="455" mass="50583" checksum="27F2A8D9480B03A7" modified="2017-12-20" version="1">MANVKVIVHEDPDLGFGPDEDDSESDSRDDSDSGSILSDDSVLPDYKLEDNMEGTVNTLYQACAKNHTSSLCKMLEQGVTQEQVMELDINGRNGLMLAVSKGFVDIVYGLNGCPFLDINHQDNDGNTALMIAAQAGFVTILTYILNYYSGVDIELRDHRGFTALIKAALQGNNDCVASLLMAGADINSVDATQRKDVREWALKTGHFETFMRLTQLSSRPRAEQFCETYVPEWPSLKELVRKATTTKSAGQKVAHRLKATFTFSFPQDPQENGVLDHMVRITTSIHSPLVVTGCRPLCPMSPPEMGKRHLAMPRPMQQNPDHKLKNHSNGSIFSASSTISAGSSVSQASCYSDTEHKSSMFSRASSRIRRLVPHSIACHNSIFPTSCIPQIKVTKSADPTPKKEKKRKMTKGYLEPPVWKYKEAKMEKKKEKQQKEKEKACKTNTEKSAKKKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SJN7</accession>
    <name>A0A2D0SJN7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">plus-end-directed kinesin ATPase</fullName>
            <ecNumber evidence="17">5.6.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">kif1b</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O + a kinesin associated with a microtubule at position (n) = ADP + phosphate a kinesin associated with a microtubule at position (n+1, toward the plus end).</text>
            <dbReference type="EC" id="5.6.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.3" evidence="17"/>
    <dbReference type="RefSeq" id="XP_017342902.1">
        <property type="nucleotide sequence ID" value="XM_017487413.3"/>
    </dbReference>
    <dbReference type="GeneID" id="108276079"/>
    <dbReference type="KEGG" id="ipu:108276079"/>
    <dbReference type="CTD" id="23095"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010970">
        <property type="term" value="P:transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01365">
        <property type="entry name" value="KISc_KIF1A_KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01233">
        <property type="entry name" value="PH_KIFIA_KIFIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000023">
        <property type="entry name" value="Kinesin family member 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000001">
        <property type="entry name" value="Kinesin family member 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000004">
        <property type="entry name" value="Kinesin-like protein isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022164">
        <property type="entry name" value="Kinesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022140">
        <property type="entry name" value="Kinesin-like_KIF1-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032405">
        <property type="entry name" value="Kinesin_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049780">
        <property type="entry name" value="PH_KIFIA_KIFIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117:SF4">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF1B ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12473">
        <property type="entry name" value="DUF3694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12423">
        <property type="entry name" value="KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16183">
        <property type="entry name" value="Kinesin_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11 18" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="Kinesin motor" evidence="21">
        <location>
            <begin position="5"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="20">
        <location>
            <begin position="1697"/>
            <end position="1795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="422"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="717"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="938"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1520"/>
            <end position="1539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1546"/>
            <end position="1567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1614"/>
            <end position="1658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="722"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="938"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1521"/>
            <end position="1539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1554"/>
            <end position="1563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1615"/>
            <end position="1634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1646"/>
            <end position="1658"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="97"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050273"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00066390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
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    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017342902.1"/>
        </source>
    </evidence>
    <sequence length="1812" mass="203656" checksum="E00802C63FE81F30" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SL69</accession>
    <name>A0A2D0SL69_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Interleukin-17 receptor D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">si:ch211-207e14.4</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017487742.3"/>
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        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030368">
        <property type="term" value="F:interleukin-17 receptor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2160">
        <property type="entry name" value="Interleukin-17 receptor A/B, fibronectin-III-like domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039465">
        <property type="entry name" value="IL-17_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031951">
        <property type="entry name" value="IL17R_D_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038683">
        <property type="entry name" value="IL17RA/B_FnIII-like_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013568">
        <property type="entry name" value="SEFIR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15583">
        <property type="entry name" value="INTERLEUKIN-17 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15583:SF17">
        <property type="entry name" value="INTERLEUKIN-17 RECEPTOR D ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16742">
        <property type="entry name" value="IL17R_D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08357">
        <property type="entry name" value="SEFIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51534">
        <property type="entry name" value="SEFIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012338793" evidence="10">
        <location>
            <begin position="23"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="317"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="SEFIR" evidence="11">
        <location>
            <begin position="381"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017343231.1"/>
        </source>
    </evidence>
    <sequence length="576" mass="65236" checksum="45D8B0CCE696814E" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SLH8</accession>
    <name>A0A2D0SLH8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA methyltransferase</fullName>
            <ecNumber evidence="6">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 11 12" type="primary">mepceb</name>
    </gene>
    <organism evidence="9 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11 12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11 12">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="6"/>
    <dbReference type="RefSeq" id="XP_017343555.1">
        <property type="nucleotide sequence ID" value="XM_017488066.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017343556.1">
        <property type="nucleotide sequence ID" value="XM_017488067.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017343557.1">
        <property type="nucleotide sequence ID" value="XM_017488068.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SLH8"/>
    <dbReference type="GeneID" id="108276418"/>
    <dbReference type="KEGG" id="ipu:108276418"/>
    <dbReference type="CTD" id="100151157"/>
    <dbReference type="OrthoDB" id="10017101at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017069">
        <property type="term" value="F:snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040031">
        <property type="term" value="P:snRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039772">
        <property type="entry name" value="Bin3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010675">
        <property type="entry name" value="Bin3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024160">
        <property type="entry name" value="BIN3_SAM-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12315:SF0">
        <property type="entry name" value="7SK SNRNA METHYLPHOSPHATE CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12315">
        <property type="entry name" value="BICOID-INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06859">
        <property type="entry name" value="Bin3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51515">
        <property type="entry name" value="BIN3_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Bin3-type SAM" evidence="8">
        <location>
            <begin position="387"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="275"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="634"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="285"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="301"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017343555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017343556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017343557.1"/>
        </source>
    </evidence>
    <sequence length="657" mass="73122" checksum="7D479F64DA719045" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SLM9</accession>
    <name>A0A2D0SLM9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">High mobility group protein B2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">High mobility group protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">hmgb2b</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">SDA103</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the HMGB family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017343614.1">
        <property type="nucleotide sequence ID" value="XM_017488125.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SLM9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005467"/>
    <dbReference type="GeneID" id="108276448"/>
    <dbReference type="KEGG" id="ipu:108276448"/>
    <dbReference type="CTD" id="447936"/>
    <dbReference type="OMA" id="IPPKGMG"/>
    <dbReference type="OrthoDB" id="1919336at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21978">
        <property type="entry name" value="HMG-box_HMGB_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21979">
        <property type="entry name" value="HMG-box_HMGB_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000006">
        <property type="entry name" value="High mobility group protein B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000018">
        <property type="entry name" value="High mobility group protein B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF3">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09011">
        <property type="entry name" value="HMG_box_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00886">
        <property type="entry name" value="HIGHMOBLTY12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="17" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-0391">Immunity</keyword>
    <keyword evidence="18" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="9" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0558">Oxidation</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <keyword evidence="16" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="23">
        <location>
            <begin position="8"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="HMG box" evidence="23">
        <location>
            <begin position="96"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="71"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="163"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="22">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00040400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00041514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_017343614.1"/>
        </source>
    </evidence>
    <sequence length="215" mass="24354" checksum="71A876BB41754234" modified="2017-12-20" version="1">MVKDANKPKGRTSAYAFFILSCREEHKKKSPGTTVNFTKFSKTCSERWKTLAATEKKKFEDLARADKDRYDREMKNYIPPKGMGKKERKKKDPNAPKRPPSAFFVFCSEHRPTVKGEYPSLTIGDIAKKLAEMWGKQSPKDRTPYEQKAAALRQKYEKDVAAYRAKGGAGGGASKRGPGRPTGSTRKPMVEADDDDDEDDEDEEEDDDEEDEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SMI2</accession>
    <name>A0A2D0SMI2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Bone morphogenetic protein 10 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC108276621</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017343912.1">
        <property type="nucleotide sequence ID" value="XM_017488423.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SMI2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007391"/>
    <dbReference type="GeneID" id="108276621"/>
    <dbReference type="KEGG" id="ipu:108276621"/>
    <dbReference type="OMA" id="FQHKYEG"/>
    <dbReference type="OrthoDB" id="5987191at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072359">
        <property type="term" value="P:circulatory system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035239">
        <property type="term" value="P:tube morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000001">
        <property type="entry name" value="Bone morphogenetic protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF39">
        <property type="entry name" value="BONE MORPHOGENETIC PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0339">Growth factor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012903730" evidence="9">
        <location>
            <begin position="31"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="10">
        <location>
            <begin position="336"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017343912.1"/>
        </source>
    </evidence>
    <sequence length="448" mass="50682" checksum="A3155849618DBA23" modified="2017-12-20" version="1">MAVSEMSVLGTRYVRSLILCILLLPRCGQTSPIPSLDEAFSGGLGAVDASTLDRDDNVDVQALLGQFLNVLNLTAHEPKPRPRAARIEPPEYMLEMYNHYTKDRSTRPAANIARSFKNEDSSPCSVTRRGVIMYPLMFNISVPRQERIIAAELRLYMLVHRDPQRDNGVDWKVTVFERQVEGGPSFRRSEGETVETNLTSMQEIVVRHGQVKESGWEVFDLTDAVLRWRDLERTSQWLELQVESENDSELQDEDKSAIFISLDIERSVGGKHEPVLIVFSDNESDKDQSKKTTEDNTGLLELSELLLDENDDSGKQQNEALLMQMRSNMIYDSAPRTRRNAKGSQCKRTALYVDFKEIGWDSWIVAPPGFEAYTCHGVCNYPLLPQVTPTKHAIIQTLVNLKSPQKVSQACCVPTKLEPISLLYENENGHVIFQHKYEGMVVAECGCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SV15</accession>
    <name>A0A2D0SV15_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">BCL2-associated athanogene 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15 16 17" type="primary">bag6</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15 16 17" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15 16 17">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
            <location evidence="3">Extracellular exosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017346532.1">
        <property type="nucleotide sequence ID" value="XM_017491043.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017346533.1">
        <property type="nucleotide sequence ID" value="XM_017491044.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017346534.1">
        <property type="nucleotide sequence ID" value="XM_017491045.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000034779"/>
    <dbReference type="GeneID" id="108277959"/>
    <dbReference type="KEGG" id="ipu:108277959"/>
    <dbReference type="CTD" id="7917"/>
    <dbReference type="OrthoDB" id="1885901at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071818">
        <property type="term" value="C:BAT3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="UBIQUITIN_2"/>
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    <keyword evidence="7" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="13">
        <location>
            <begin position="9"/>
            <end position="70"/>
        </location>
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            <begin position="80"/>
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            <begin position="215"/>
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            <begin position="261"/>
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            <dbReference type="Proteomes" id="UP000221080"/>
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    <sequence length="1305" mass="136878" checksum="9B8549E4D2E0F91C" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SXC3</accession>
    <name>A0A2D0SXC3_ICTPU</name>
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            <fullName evidence="4 13">Small nuclear ribonucleoprotein Sm D3</fullName>
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            <fullName evidence="11 13">snRNP core protein D3</fullName>
        </alternativeName>
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        <name evidence="16" type="primary">snrpd3l</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
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    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs. As part of the U7 snRNP it is involved in histone pre-mRNA 3'-end processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the snRNP core protein family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01721">
        <property type="entry name" value="Sm_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Small nuclear ribonucleoprotein Sm D3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LSm4/Sm_D1/D3"/>
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        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Sm"/>
    </dbReference>
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        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034099">
        <property type="entry name" value="SmD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8 13" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13 16" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="14">
        <location>
            <begin position="5"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00033126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017347334.1"/>
        </source>
    </evidence>
    <sequence length="128" mass="13948" checksum="B52A22F3EF936B82" modified="2017-12-20" version="1">MSIGVPIKVLHEAEGHIVTCETNTGEVYRGKLIEAEDNMNCQMSNITVTYRDGRVAQLEQVYIRGSKIRFLILPDMLKNAPMLKSVKNKNQGAGAGRGKAAILKAQVAARGRGRGGPGGRGNIFQKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SY14</accession>
    <name>A0A2D0SY14_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein si:dkeyp-72g9.4 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">si:dkeyp-72g9.4</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017347579.1">
        <property type="nucleotide sequence ID" value="XM_017492090.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A2D0SY14"/>
    <dbReference type="GeneID" id="108278628"/>
    <dbReference type="KEGG" id="ipu:108278628"/>
    <dbReference type="OrthoDB" id="9891865at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
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    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="DEPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15426">
        <property type="entry name" value="PROTEIN DEPP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15426:SF6">
        <property type="entry name" value="PROTEIN DEPP1"/>
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            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="139"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017347579.1"/>
        </source>
    </evidence>
    <sequence length="277" mass="30818" checksum="AD4153077507BBCC" modified="2017-12-20" version="1">MIVRSYGDGNEPVALFLAQAKGGWRKQAGEQMMMRPRSRLLAKRGLPTIREGSEELVHDLNQTNSQHSSAQDYFHSICQLAQPTFPLHEPDRDILSIGRLDSPKPCLRPHRLRQLAQPLTCPTDTSTTQSPKVEAPGPSSSEQEQAQGSEQDCPTSTDPLEYLYGYREAEASSSSTRRVSIPLRRARSVPCPGLDVQNKSSCPDLRLPESVLSPVVPQRSPLSRKKLDGANVECGWRAKSSRSLDKQTIVSHWISECRSAWREARIHACMLPAIAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979ECG3</accession>
    <name>A0A979ECG3_ICTPU</name>
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        <submittedName>
            <fullName evidence="4">Carcinoembryonic antigen-related cell adhesion molecule 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC108277545</name>
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    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047007224.1">
        <property type="nucleotide sequence ID" value="XM_047151268.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979ECG3"/>
    <dbReference type="GeneID" id="108277545"/>
    <dbReference type="KEGG" id="ipu:108277545"/>
    <dbReference type="OrthoDB" id="8439544at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="243"/>
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    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="156"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_047007224.1"/>
        </source>
    </evidence>
    <sequence length="255" mass="29329" checksum="8030D8232B3AD326" modified="2023-02-22" version="1">MTREDCFVDEMRMQVIIGILWMFPGVVMGLLFQDPVVCRFTETNQCNVALGQRLHLQMVEEDLFYLILSNNHFILKYKKNQLNLPKPNPPRWQFVNDNKTMILSSAERNDSGKYTLVINKADGNITTFYTLQLNIEAEVSSVKVSYSCLSPGIRKVYCSADGDNLHFNWTSDLNPVTQLEDGNSTFLLEQNHGKVTCHVENHVSSAQNSTKLYPCIGFMVFVTVWLFEVIILLLLLVGTFYIYTRMYRKQSVNVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EP26</accession>
    <name>A0A979EP26_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transmembrane protease serine 13b isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tmprss13b</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047007893.1">
        <property type="nucleotide sequence ID" value="XM_047151937.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EP26"/>
    <dbReference type="GeneID" id="108259860"/>
    <dbReference type="CTD" id="564693"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF7">
        <property type="entry name" value="HYALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15494">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 6 10" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="244"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_047007893.1"/>
        </source>
    </evidence>
    <sequence length="482" mass="52142" checksum="63ECDAF1AB1D0B74" modified="2023-02-22" version="1">MENTEVNVEHGENPPPYYLVVKPSCPPPPYAMVQPLPSTPPVVPYSNQPLPQLLAPHVPQRVITTIEQDPRQSRLVVTRHKKARCYGGSGGSLLILLFTGIVIWLGVRYGVSVMSRGKSDTCPPNTVVCDGHTDCQKGSDETICVRFGEGNELQVMTSNSSGFLPICYADWNHSLATQTCQQLGFRGSYKYGGLTSTSKSFLFVTSESSDTIQGKVNVGTMCPGEQATFLQCTDCGLPRTSSRIIGGSVATPGLWPWQASLHFEGSHVCGGSVVSQDFIVSAAHCFPSDPASWQLAVNWRVYLGVESQYMLPPPHYVADIIIHESYNPNTHDNDVALLKLTQPINFTGSVQPVCLPAFDQVISTDTQCWITGFGTTQEGGALGSSQLMEVAVDIIDTSVCNDAYKGLISNDMLCAGDMEGGRDACQGDSGGPLVCQEEDGRWYLYGVTSWGSGCGRVNSPGVYSNVHSLLAWIHSKMEEDRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EVN6</accession>
    <name>A0A979EVN6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Homeobox protein Hox-D3a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">hoxd3a</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Antp homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047011926.1">
        <property type="nucleotide sequence ID" value="XM_047155970.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EVN6"/>
    <dbReference type="GeneID" id="108266964"/>
    <dbReference type="KEGG" id="ipu:108266964"/>
    <dbReference type="CTD" id="30349"/>
    <dbReference type="OMA" id="YPYGAAH"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048704">
        <property type="term" value="P:embryonic skeletal system morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000094">
        <property type="entry name" value="Homeobox protein Hox-A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025281">
        <property type="entry name" value="DUF4074"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45664:SF5">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45664">
        <property type="entry name" value="PROTEIN ZERKNUELLT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13293">
        <property type="entry name" value="DUF4074"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="158"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="160"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="48"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="132"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="218"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="363"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="135"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="374"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_047011926.1"/>
        </source>
    </evidence>
    <sequence length="395" mass="43152" checksum="D11B410E8B5F4246" modified="2023-02-22" version="1">MQKATYYDNAGLFGGYSYSKSDSYAYGPAHQSYPTSSIENDYQGPICSVQSTTVRPPVHKNSDINGSCMRPNGSQGSQPESVGDQQQASPLAPTSPNSSSTTTQKKKSPNNNGSNTATPVITKQIFPWMKETRQNSKQKSTNCTTPGETCDDKSPPGPASKRVRTAYTSAQLVELEKEFHFNRYLCRPRRVEMANLLNLTERQIKIWFQNRRMKYKKDQKSKGIMHSPLGHSPDRSPPLSGPNHVGYSGQLPNVNSLNYDAPSPPSFAKPQQNMYGLAAYTAPLGGCIPQQKRYPGAEYEHHGMQGNGSFANANLQGSPVYVGGNFVDSMPASGPMFNLGHLPHPSSASVDYSCAAQIPGNHHHGPCDPHPTYTDLSSHHTSQGRLQEAPKLTHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F1M1</accession>
    <name>A0A979F1M1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">E3 ubiquitin/ISG15 ligase TRIM25-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC108269831</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047013819.1">
        <property type="nucleotide sequence ID" value="XM_047157863.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979F1M1"/>
    <dbReference type="GeneID" id="108269831"/>
    <dbReference type="KEGG" id="ipu:108269831"/>
    <dbReference type="OrthoDB" id="265776at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF32">
        <property type="entry name" value="BLOODTHIRSTY-RELATED GENE FAMILY, MEMBER 16 ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_BBOX"/>
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        <property type="entry name" value="ZF_RING_1"/>
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        <property type="entry name" value="ZF_RING_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="15"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="6">
        <location>
            <begin position="148"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_047013819.1"/>
        </source>
    </evidence>
    <sequence length="239" mass="26932" checksum="7A7E6872B44B176F" modified="2023-02-22" version="1">MASSSSVLSEDELQCSICLDVFTDPVSTPCGHNFCKVCLRKCWDTCPHCQCPVCKEAFPKRPELRVNTFISALATQFKKSVQVKSSSAADPLLSMTKKVLCDSCTEEKREALKSCLHCGVSLCSTHLLFHKTTAKFKRHKLIDPVENLEDYICQKHDRPLELFCRDDQTCVCQFCIEGDHKTHNTVPIQVFMKICNNQQTYGEETSVETVKDCGSESAQMELRQLTGTCSPGRCIIWIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F2V4</accession>
    <name>A0A979F2V4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Dedicator of cytokinesis protein 7 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">dock7</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the DOCK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047014393.1">
        <property type="nucleotide sequence ID" value="XM_047158437.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108271855"/>
    <dbReference type="CTD" id="85440"/>
    <dbReference type="OrthoDB" id="47328at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08696">
        <property type="entry name" value="C2_Dock-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11703">
        <property type="entry name" value="DHR2_DOCK7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.740:FF:000002">
        <property type="entry name" value="Dedicator of cytokinesis protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.410:FF:000002">
        <property type="entry name" value="Dedicator of cytokinesis protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dedicator of cytokinesis protein 7"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037808">
        <property type="entry name" value="C2_Dock-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027007">
        <property type="entry name" value="C2_DOCK-type_domain"/>
    </dbReference>
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        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021816">
        <property type="entry name" value="DOCK_C/D_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043161">
        <property type="entry name" value="DOCK_C_lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043162">
        <property type="entry name" value="DOCK_C_lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027357">
        <property type="entry name" value="DOCKER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046769">
        <property type="entry name" value="DOCKER_Lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046770">
        <property type="entry name" value="DOCKER_Lobe_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046773">
        <property type="entry name" value="DOCKER_Lobe_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23317:SF78">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06920">
        <property type="entry name" value="DHR-2_Lobe_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20422">
        <property type="entry name" value="DHR-2_Lobe_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20421">
        <property type="entry name" value="DHR-2_Lobe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14429">
        <property type="entry name" value="DOCK-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11878">
        <property type="entry name" value="DOCK_C-D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51650">
        <property type="entry name" value="C2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 DOCK-type" evidence="5">
        <location>
            <begin position="559"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="domain" description="DOCKER" evidence="6">
        <location>
            <begin position="1698"/>
            <end position="2134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="136"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="888"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1449"/>
            <end position="1468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="888"/>
            <end position="899"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_047014393.1"/>
        </source>
    </evidence>
    <sequence length="2160" mass="245598" checksum="2E4AFF084C597330" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979FEA6</accession>
    <name>A0A979FEA6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Schwannomin-interacting protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">schip1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047018584.1">
        <property type="nucleotide sequence ID" value="XM_047162628.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979FEA6"/>
    <dbReference type="GeneID" id="108280660"/>
    <dbReference type="CTD" id="29970"/>
    <dbReference type="OrthoDB" id="6260144at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035332">
        <property type="term" value="P:positive regulation of hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029362">
        <property type="entry name" value="IQCJ-SCHIP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039045">
        <property type="entry name" value="SCHIP_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015649">
        <property type="entry name" value="SCHIP_1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13103">
        <property type="entry name" value="SCHWANNOMIN INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13103:SF4">
        <property type="entry name" value="SCHWANNOMIN-INTERACTING PROTEIN 1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15157">
        <property type="entry name" value="IQCJ-SCHIP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10148">
        <property type="entry name" value="SCHIP-1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fusion protein IQCJ-SCHIP1 N-terminal" evidence="4">
        <location>
            <begin position="154"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Fusion protein IQCJ-SCHIP1 N-terminal" evidence="4">
        <location>
            <begin position="314"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Schwannomin interacting protein 1 C-terminal" evidence="3">
        <location>
            <begin position="570"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="172"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="331"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="461"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="554"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="623"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="739"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="342"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="375"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="559"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="624"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="639"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="659"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_047018584.1"/>
        </source>
    </evidence>
    <sequence length="822" mass="92201" checksum="C0A68CDBB639CCA8" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R0Z2</accession>
    <name>A0A9F7R0Z2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">tRNA (guanine(27)-N(2))-dimethyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">tRNA methyltransferase 1-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">trmt1l</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>guanosine(27) in tRNA(Tyr) + 2 S-adenosyl-L-methionine = N(2)-dimethylguanosine(27) in tRNA(Tyr) + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:83895"/>
            <dbReference type="Rhea" id="RHEA-COMP:20240"/>
            <dbReference type="Rhea" id="RHEA-COMP:20241"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:83896"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053531705.1">
        <property type="nucleotide sequence ID" value="XM_053675730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R0Z2"/>
    <dbReference type="GeneID" id="108257710"/>
    <dbReference type="KEGG" id="ipu:108257710"/>
    <dbReference type="CTD" id="81627"/>
    <dbReference type="OrthoDB" id="6349953at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016423">
        <property type="term" value="F:tRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002940">
        <property type="term" value="P:tRNA N2-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000098">
        <property type="entry name" value="Trmt1-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.70:FF:000001">
        <property type="entry name" value="tRNA (guanine(26)-N(2))-dimethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.70">
        <property type="entry name" value="N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002905">
        <property type="entry name" value="Trm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042296">
        <property type="entry name" value="tRNA_met_Trm1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631">
        <property type="entry name" value="N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631:SF1">
        <property type="entry name" value="TRMT1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02005">
        <property type="entry name" value="TRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51626">
        <property type="entry name" value="SAM_MT_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 19" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 19" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 19" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 19" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4 19" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="21">
        <location>
            <begin position="196"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="705"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="705"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00093188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00093671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_053531705.1"/>
        </source>
    </evidence>
    <sequence length="725" mass="80768" checksum="504AE74B180754FD" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R479</accession>
    <name>A0A9F7R479_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GTPase-activating protein and VPS9 domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">gapvd1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GAPVD1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053536199.1">
        <property type="nucleotide sequence ID" value="XM_053680224.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108265693"/>
    <dbReference type="KEGG" id="ipu:108265693"/>
    <dbReference type="CTD" id="26130"/>
    <dbReference type="OrthoDB" id="10264848at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:GTPase activator activity"/>
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        <property type="term" value="F:small GTPase binding"/>
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        <property type="term" value="P:endocytosis"/>
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        <property type="term" value="P:regulation of transport"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="DUF5601"/>
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        <property type="entry name" value="VPS9"/>
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    </dbReference>
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        <property type="entry name" value="RAB GDP/GTP EXCHANGE FACTOR"/>
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        <property type="entry name" value="DUF5601"/>
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    </dbReference>
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        <property type="entry name" value="VPS9"/>
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    <dbReference type="SMART" id="SM00167">
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    </dbReference>
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        <location>
            <begin position="149"/>
            <end position="353"/>
        </location>
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        <location>
            <begin position="1350"/>
            <end position="1490"/>
        </location>
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        <location>
            <begin position="480"/>
            <end position="502"/>
        </location>
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        <location>
            <begin position="569"/>
            <end position="604"/>
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        <location>
            <begin position="1069"/>
            <end position="1126"/>
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            <begin position="575"/>
            <end position="585"/>
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            <begin position="754"/>
            <end position="768"/>
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            <begin position="882"/>
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        </location>
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            <begin position="910"/>
            <end position="930"/>
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            <begin position="1071"/>
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            <dbReference type="Proteomes" id="UP000221080"/>
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        <source>
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    </evidence>
    <sequence length="1490" mass="166193" checksum="D9CFBD5D902FC6C5" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R4C8</accession>
    <name>A0A9F7R4C8_ICTPU</name>
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        <submittedName>
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        </submittedName>
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        <name evidence="2" type="primary">LOC128632764</name>
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            <taxon>Vertebrata</taxon>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SDA103</strain>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_053535945.1"/>
        </source>
    </evidence>
    <sequence length="200" mass="22082" checksum="D3007F9FE245A966" modified="2023-06-28" version="1">MILYKTPSTAWSDSAVVQTRMVFNSSSPVPSERLVLSAIQTLLSARLTNLIHSVKVLNFTYEKISDTSYAVNFTLRISNISMSKNPDVRNDTYTQVENIINNALNTLLNDAGAEPFEPQGSFFMSSGNQVNGDMDYYFQDGDNKTPAAFLNELTPVYSSAGSAVVQSRMVFNSSSPVPSESLVLNTMRKFQTPPMQLTSH</sequence>
</entry>
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    <accession>A0A9F7R5X0</accession>
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    </protein>
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        <name evidence="9 10 11" type="primary">LOC128628775</name>
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    <organism evidence="8 11">
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9 10 11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10 11">Blood</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053537112.1">
        <property type="nucleotide sequence ID" value="XM_053681137.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053537113.1">
        <property type="nucleotide sequence ID" value="XM_053681138.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_053681139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R5X0"/>
    <dbReference type="GeneID" id="128628775"/>
    <dbReference type="KEGG" id="ipu:128628775"/>
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        <property type="component" value="Chromosome 7"/>
    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="Compl-Cell_Adhes-Reg"/>
    </dbReference>
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        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19325">
        <property type="entry name" value="COMPLEMENT COMPONENT-RELATED SUSHI DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19325:SF575">
        <property type="entry name" value="LOCOMOTION-RELATED PROTEIN HIKARU GENKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="1 5" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044698414" evidence="6">
        <location>
            <begin position="21"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="22"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="80"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="141"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="206"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="265"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="7">
        <location>
            <begin position="324"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="82"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="208"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_053537112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053537113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053537114.1"/>
        </source>
    </evidence>
    <sequence length="405" mass="43563" checksum="1DAA201E8A655F47" modified="2023-06-28" version="1">MKKKIIILAFSVWSCQAVMGQSSCTAPVVPNAKPKNELAASYPTGSFIRYVCDSGYEFVETHYALCEDGTWNLPVCKLQTSCTVPFVPNAELADEFTAALYPTGSSVRFVCDRGSEFEGTKYALCKDGTWKLPVCKILDHARCASPRVPNARPADALAKSYPSGSLVRFGCDRGYGFEGTDSAQCEEGVWTLPVCKDVPFLVLDQAPCSAAPRVPNARPADALAESYPSGSSVRFGCDGGYEFEGTDSAQCEDGVWTLPVCKDVKSCLAPSVPNGQPANALKTLYGPGETLRFVCDSGYEFEGTRYAVCDDGSWRLPVCKVLDAACAAPHVPNAKPENELAASYPAGSTVRFGCDGGYEIEGTRYALCEDGTWRLPVCTLPCTLDRTRFTSSQADEGGRREDVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R6J8</accession>
    <name>A0A9F7R6J8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="1">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC128629614</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="1"/>
    <dbReference type="RefSeq" id="XP_053534322.1">
        <property type="nucleotide sequence ID" value="XM_053678347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R6J8"/>
    <dbReference type="GeneID" id="128629614"/>
    <dbReference type="KEGG" id="ipu:128629614"/>
    <dbReference type="OrthoDB" id="26387at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF28">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053534322.1"/>
        </source>
    </evidence>
    <sequence length="362" mass="40223" checksum="0D802310DA8AAE3B" modified="2023-06-28" version="1">MSTSGGPTPPFTTLSVTHPANHITPSNALIRLLKTQSAVWSRVQGKSSLQAEEAQRKLKRQNGEDPALPPFCPLFASLGNILQCDVLLGMLGAVLQWAMEPSGGHWSESMLQRVLHLIGMALLEEQQQLENIGDDDEVTFNFTLKISRPGEAPGNTLSILALLETLQCAPHLEAHKDMIRWILKMVVSIKTMRERTAAAPPCEGTGHCHKASVSTWYSPSSCDSALVCMGPRRWRARGGERRQMEMCILCHEAQEILPDGTAMVLAAFVQRSTVMSEIRKRPPHNPESYDPLFMHPDLSFGTHTGSCGHIMHSHCWQRYFETLQSQDAQWLHEQTSCDVVNGEYLCPLCKCRSNTHSSPAPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RFF8</accession>
    <name>A0A9F7RFF8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein DBF4 homolog A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">dbf4b</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053541206.1">
        <property type="nucleotide sequence ID" value="XM_053685231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RFF8"/>
    <dbReference type="GeneID" id="108273428"/>
    <dbReference type="KEGG" id="ipu:108273428"/>
    <dbReference type="CTD" id="80174"/>
    <dbReference type="OrthoDB" id="21380at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031431">
        <property type="term" value="C:Dbf4-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010571">
        <property type="term" value="P:positive regulation of nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901987">
        <property type="term" value="P:regulation of cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3410:FF:000001">
        <property type="entry name" value="Protein DBF4 homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3410">
        <property type="entry name" value="DBF zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051590">
        <property type="entry name" value="Replication_Regulatory_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006572">
        <property type="entry name" value="Znf_DBF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038545">
        <property type="entry name" value="Znf_DBF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15375">
        <property type="entry name" value="ACTIVATOR OF S-PHASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15375:SF24">
        <property type="entry name" value="PROTEIN DBF4 HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07535">
        <property type="entry name" value="zf-DBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00586">
        <property type="entry name" value="ZnF_DBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51265">
        <property type="entry name" value="ZF_DBF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DBF4-type" evidence="6">
        <location>
            <begin position="255"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="209"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="321"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="321"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="372"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="413"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="436"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053541206.1"/>
        </source>
    </evidence>
    <sequence length="747" mass="82004" checksum="368C200EBF941297" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RH12</accession>
    <name>A0A9F7RH12_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Membrane-spanning 4-domains subfamily A member 12 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC128628794</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MS4A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053540007.1">
        <property type="nucleotide sequence ID" value="XM_053684032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RH12"/>
    <dbReference type="GeneID" id="128628794"/>
    <dbReference type="KEGG" id="ipu:128628794"/>
    <dbReference type="OrthoDB" id="10071849at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007237">
        <property type="entry name" value="CD20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030417">
        <property type="entry name" value="MS4A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320:SF128">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MEMBER 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MS4A -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04103">
        <property type="entry name" value="CD20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053540007.1"/>
        </source>
    </evidence>
    <sequence length="248" mass="25911" checksum="BCBB62B8E670BF1F" modified="2023-06-28" version="1">MASAPIPLPNVGSGYTIVTQVLPSSTSSNTADQNSPQMLGPLRKFLKGEPKALGTVQIMIGLLTILLGIVMAVYPQNISVYSGVIFWGSLLHITAGALAVSASNKLNACVVKGAMVVNIFSTIAAGIAIIMLSLDLVIGPHRLCLFNGCGNDFIAVSRTSGIIGVLLVFSLLQFTISIAISAFTCKATCTNEPTLNIINVVPNPEGWVPVVSSFPAHHAQPGDNTMNAFTMSSAPPAYSEKSQPDNRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RN74</accession>
    <name>A0A9F7RN74_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">HAUS augmin-like complex subunit 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">haus1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAUS1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053543607.1">
        <property type="nucleotide sequence ID" value="XM_053687632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RN74"/>
    <dbReference type="GeneID" id="108278823"/>
    <dbReference type="KEGG" id="ipu:108278823"/>
    <dbReference type="CTD" id="115106"/>
    <dbReference type="OrthoDB" id="5372507at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070652">
        <property type="term" value="C:HAUS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007098">
        <property type="term" value="P:centrosome cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026243">
        <property type="entry name" value="HAUS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31570">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31570:SF1">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25762">
        <property type="entry name" value="HAUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02087">
        <property type="entry name" value="HAUSAUGMINL1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="120"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053543607.1"/>
        </source>
    </evidence>
    <sequence length="282" mass="31434" checksum="EEDC50EB5962A0E7" modified="2023-06-28" version="1">MEDTGTSSYRKVNRWLETVFGAEGVPGFEVNTRTVELLDQLAQISELRCSEVSLLTDDYRHKAAEYSSDAAHLQEVLHQGVGLQPGGVPKSTSDFLSVLEGTAEVLKVKDTSMGSFVPAINKLTNDLAEAEKTDRKLGQELAAVRKKMAATMILRKKLQEDLNKIAQVQQVEAATAEERLLNMDFMKNKSRDLTYRNKIAQDKLASRQMKDSLTHQAIVQLAEKITALKQEIAPLLKKLEPFNDLSPSPALARVAIEEVKRELEALDAELEQKVDLMNTLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RNP9</accession>
    <name>A0A9F7RNP9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein F54H12.2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC128635357</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SDA103</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053543937.1">
        <property type="nucleotide sequence ID" value="XM_053687962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RNP9"/>
    <dbReference type="GeneID" id="128635357"/>
    <dbReference type="KEGG" id="ipu:128635357"/>
    <dbReference type="OrthoDB" id="8869837at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000358">
        <property type="entry name" value="RNR_small_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409:SF21">
        <property type="entry name" value="CAPSID PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_053543937.1"/>
        </source>
    </evidence>
    <sequence length="378" mass="42066" checksum="6892A10B82449DAB" modified="2023-06-28" version="1">MARMALKIRIVDRPERIGRYGTDIADGAPVGLINYAGSTIFSQVDVSLGDRLISQSSNTYPYRCIIECLTNYGKDALETLFSAGLFYKDTAGHMDVTDPAGGKRGLTKRAAFTNASNVVELLAPIHSDIFFQEKLMLNGVDIKIRMTRAKDEFCLMRSGAVAYKLNIVSASLFVKKVSVSPGVRLGHAQALLSTPAKYPMDRVCVKIFSIPVGSRVCNQENLFLGTLPKSVVLAMVDNDAFTGSYDKNPFAFKNYDLEFLAVYLDGQQRPAKPLQPEYGSGSAVREFYQLALASGKHLKNQPLAIDREDFLHGYTLYAFNLTPDEDCSQHVSLIKSGNIRLEARFRQPLPRTINLIVYAIFDSIIEVSNRRQILVDYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RNX7</accession>
    <name>A0A9F7RNX7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="1">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC128634128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="1"/>
    <dbReference type="RefSeq" id="XP_053540373.1">
        <property type="nucleotide sequence ID" value="XM_053684398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RNX7"/>
    <dbReference type="GeneID" id="128634128"/>
    <dbReference type="KEGG" id="ipu:128634128"/>
    <dbReference type="OrthoDB" id="26387at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF28">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053540373.1"/>
        </source>
    </evidence>
    <sequence length="137" mass="15378" checksum="FE79144B126E323D" modified="2023-06-28" version="1">MAQTLQSQSHFINKYIDLLTQDSEDLDASASTSAEHSPSSCDGALVCVGPRRWRARGGERRQMEMCILCHEAQEILPDGTAMVLAAFVQRSTVMSKNRKRPPHNPESYDPLFMHPDLSFGTHTDSCGHIMHSHGWQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RP61</accession>
    <name>A0A9F7RP61_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">vesicle-fusing ATPase</fullName>
            <ecNumber evidence="3">3.6.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC108270689</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.6" evidence="3"/>
    <dbReference type="RefSeq" id="XP_053539397.1">
        <property type="nucleotide sequence ID" value="XM_053683422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RP61"/>
    <dbReference type="GeneID" id="108270689"/>
    <dbReference type="KEGG" id="ipu:108270689"/>
    <dbReference type="OrthoDB" id="29072at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02678">
        <property type="entry name" value="MIT_VPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19521">
        <property type="entry name" value="RecA-like_VPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000043">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000015">
        <property type="entry name" value="vacuolar protein sorting-associated protein 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000002">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045253">
        <property type="entry name" value="VPS4_MIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF72">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04212">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00745">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MIT" evidence="16">
        <location>
            <begin position="2"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="157"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="73"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_053539397.1"/>
        </source>
    </evidence>
    <sequence length="435" mass="48873" checksum="FB9BFEA2F3C2A714" modified="2023-06-28" version="1">MANSNLQKAIDLATKASEEDKAKNYEEALRLYQHSIQYFLHAVKYEAQGEKAKQSIRAKCKDYLDRAEQLKDYLKKTEKQPPTKPVKVSNDKGNDGDDGDDSEKKKLQNQLQGAIVMEKPNIKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKNLFTLARQHKPSIIFIDEIDSLCGSRSDNESEAARRIKTEFLVQMQGVGNDNEGILVLGATNIPWTLDSAIRRRFEKRIYIPLPEQHARSFMFKLNLGTTPNNLTDNDFITLGQKTESYSGADISIIVRDALMQPIRKVQMATHFKKTCGKTWNNQDVIADDLLTPCSPSDPNAIKMTWVDVEADKLLEPIVSMEDMLRSLEKTKPTVNDEDLKKLVKFTEDFGQEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RTF1</accession>
    <name>A0A9F7RTF1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">DnaJ homolog subfamily C member 18 isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">dnajc18</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053543643.1">
        <property type="nucleotide sequence ID" value="XM_053687668.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RTF1"/>
    <dbReference type="GeneID" id="108278917"/>
    <dbReference type="CTD" id="202052"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000004">
        <property type="entry name" value="DnaJ (Hsp40) homolog, subfamily B, member 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051100">
        <property type="entry name" value="DnaJ_subfamily_B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015399">
        <property type="entry name" value="DUF1977_DnaJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908:SF2">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09320">
        <property type="entry name" value="DUF1977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="9">
        <location>
            <begin position="46"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="105"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053543643.1"/>
        </source>
    </evidence>
    <sequence length="332" mass="38659" checksum="3E18C2050B1F5E47" modified="2023-06-28" version="1">MDEDIPGRRQHQRYSAGDAGEAPRDDKNSYTEEQRQGVLRIQRCKDFYEILGVPKDASDEDLKKAYRKLALRFHPDKNCAPGATDAFKAIGNAYAVLSNPEKRQQYDEYGEQGAAETASHSSAQPRYTHTRHRTFNRDFEADISPEELFNMFFGGRFPTGNIHVYTNRGASYSNFYQPRRRRVYERREEEVQENRSQNTFTAFLQLLPVLVLILISVITQLMATNPPYSLFYKPSMGLVVSRETQHMGVQYYVDKSFEKEYHGVALDELEKTIESDYIDHLQSSCWKEKQQKSDLANLGQLYRDERLKQKAETMKLDHCEKLHRFVGRQRGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TH81</accession>
    <name>A0A9F7TH81_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Autism susceptibility gene 2 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">fbrsl1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_053535248.1">
        <property type="nucleotide sequence ID" value="XM_053679273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TH81"/>
    <dbReference type="GeneID" id="108255474"/>
    <dbReference type="KEGG" id="ipu:108255474"/>
    <dbReference type="CTD" id="57666"/>
    <dbReference type="OrthoDB" id="10060000at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023246">
        <property type="entry name" value="AUTS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14429">
        <property type="entry name" value="FIBROSIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14429:SF20">
        <property type="entry name" value="FIBROSIN-1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15336">
        <property type="entry name" value="Auts2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02044">
        <property type="entry name" value="FIBROSIN1LPF"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="255"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="661"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="294"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="340"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="367"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="404"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="451"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="490"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="661"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="685"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053535248.1"/>
        </source>
    </evidence>
    <sequence length="704" mass="78896" checksum="1D34F4384735F954" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7THY5</accession>
    <name>A0A9F7THY5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">UPF0606 protein KIAA1549 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">si:ch211-1e14.1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
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            <begin position="233"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022546"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50228"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053536539.1"/>
        </source>
    </evidence>
    <sequence length="323" mass="35951" checksum="D80685B812834179" modified="2023-06-28" version="1">MLFLKLTLLSWKIVTCEGNTAVLTCGFRRIRIISANYGRTDSITCSSGRPPSQLSNTNCYSFSSLYNVVVRCKGRYSCQVPATNSVFSDPCFGTYKYLKVAYICVQTIQSTQPGDSVTTEFFHTVITVRLFRPLLAAGDLARPLRLDECFRRIRIISANYGRTDSTICSSGRPPSQLSNTNCYSSSSLYNVVVRCELQHTCQVPATNSVFSDPCVDTYKYLKVVYICTREIVTCEGNTAVLTCGVLRIRIIRANYGRTDSTTCSSGQPPSQLSNTNCYSYNTLSLFCCRCELQQTCQVPATNSVFSDPCVGTYKYLKVVYICV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TMH6</accession>
    <name>A0A9F7TMH6_ICTPU</name>
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        <recommendedName>
            <fullName evidence="2">DNA-directed DNA polymerase</fullName>
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        <name evidence="12" type="primary">LOC128633471</name>
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    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
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            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA polymerase type-B family.</text>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VSR Endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA-dir_DNA_pol_B_mt/vir"/>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
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        <property type="entry name" value="RNaseH-like_sf"/>
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        <property type="entry name" value="RNaseH_sf"/>
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    </dbReference>
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        <property type="entry name" value="DNA-DIRECTED DNA POLYMERASE"/>
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        <property type="entry name" value="DNA_pol_B_2"/>
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    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
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            <begin position="736"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B mitochondria/virus" evidence="10">
        <location>
            <begin position="1070"/>
            <end position="1232"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF03175"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053538216.1"/>
        </source>
    </evidence>
    <sequence length="1284" mass="148944" checksum="9F9E3AE84E1FEEC4" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
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    <name>A0A9F7TQ39_ICTPU</name>
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        <submittedName>
            <fullName evidence="4">UHRF1-binding protein 1-like isoform X1</fullName>
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    <organism evidence="3 4">
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053541637.1">
        <property type="nucleotide sequence ID" value="XM_053685662.1"/>
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    <dbReference type="KEGG" id="ipu:108274761"/>
    <dbReference type="CTD" id="23074"/>
    <dbReference type="OMA" id="STAEQCH"/>
    <dbReference type="OrthoDB" id="43807at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026728">
        <property type="entry name" value="BLTP3A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22774:SF17">
        <property type="entry name" value="BRIDGE-LIKE LIPID TRANSFER PROTEIN FAMILY MEMBER 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22774">
        <property type="entry name" value="CHOREIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24917">
        <property type="entry name" value="BLTP3A_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="268"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="864"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="921"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1286"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1319"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="870"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="921"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="934"/>
            <end position="944"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053541637.1"/>
        </source>
    </evidence>
    <sequence length="1354" mass="149491" checksum="9DE88C370E43762D" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3TF58</accession>
    <name>E3TF58_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9 11">transcription initiation factor TFIID subunit 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TAF7</name>
        <name evidence="11" type="synonym">taf7</name>
        <name evidence="11" type="synonym">taf7l</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF7 family.</text>
    </comment>
    <dbReference type="EMBL" id="GU588992">
        <property type="protein sequence ID" value="ADO28944.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187656.1">
        <property type="nucleotide sequence ID" value="NM_001200727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TF58"/>
    <dbReference type="GeneID" id="100528457"/>
    <dbReference type="KEGG" id="ipu:100528457"/>
    <dbReference type="CTD" id="6879"/>
    <dbReference type="OrthoDB" id="153872at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08047">
        <property type="entry name" value="TAF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037817">
        <property type="entry name" value="TAF7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006751">
        <property type="entry name" value="TAFII55_prot_cons_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12228:SF0">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN ASSOCIATED FACTOR 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12228">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID 55 KD SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04658">
        <property type="entry name" value="TAFII55_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01370">
        <property type="entry name" value="TAFII55_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TAFII55 protein conserved region" evidence="8">
        <location>
            <begin position="22"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="201"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="291"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="203"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="220"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="255"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADO28944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="NP_001187656.1"/>
        </source>
    </evidence>
    <sequence length="362" mass="41479" checksum="81F415C4F70593AC" modified="2011-01-11" version="1">MASKTKAGKVNSKNKEDAPHELESQFVLRLPQEYASTVRRIAQSGSVNLKDRLTIELHADGRHGIVRVDRVPLACKLVDLPCILESLKTVDKKTFYKTADVCQMLVCSLDGDLYPPLEEPTGTVDPKTKKKDKDKDKKFIWNHGITCPLKNTRKRRFRKTAKKKYIESPDVEKEVKRLLSTDAEAVSVRWEVIAEDETKETDNNLSLSNLDSSPGTSGHKGHDSSVPRDELREIFNDISSSSEDEDDDVDRHEDEDLNIIDTEDDTVGRLNEKLNESDGGRDENNRNNQIVMEYQMQINNLKAKLQETRARKKQQEDLIMKVENQALKTRFQALLNDFIHQEEREMEKLASLQEQLDSMVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3TFL0</accession>
    <name>E3TFL0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">COMM domain-containing protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">COMD6</name>
        <name evidence="7" type="synonym">comd6</name>
        <name evidence="7" type="synonym">commd6</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Scaffold protein in the commander complex that is essential for endosomal recycling of transmembrane cargos; the commander complex is composed of the CCC subcomplex and the retriever subcomplex. May modulate activity of cullin-RING E3 ubiquitin ligase (CRL) complexes. Down-regulates activation of NF-kappa-B. Inhibits TNF-induced NFKB1 activation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the COMM domain-containing protein 6 family.</text>
    </comment>
    <dbReference type="EMBL" id="GU589144">
        <property type="protein sequence ID" value="ADO29096.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001188189.1">
        <property type="nucleotide sequence ID" value="NM_001201260.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TFL0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021116"/>
    <dbReference type="GeneID" id="100528990"/>
    <dbReference type="KEGG" id="ipu:100528990"/>
    <dbReference type="CTD" id="170622"/>
    <dbReference type="OrthoDB" id="10251827at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051059">
        <property type="term" value="F:NF-kappaB binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017920">
        <property type="entry name" value="COMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047155">
        <property type="entry name" value="COMMD4/6/7/8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16231">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 4-8 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16231:SF5">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07258">
        <property type="entry name" value="COMM_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51269">
        <property type="entry name" value="COMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COMM" evidence="4">
        <location>
            <begin position="128"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00039908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00093393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00093468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADO29096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="NP_001188189.1"/>
        </source>
    </evidence>
    <sequence length="195" mass="21909" checksum="C47D4FC5DD109D83" modified="2011-01-11" version="1">MELSSDVAVVENIGKLPSDMFEGTCHEILLHLEGKRSAVDVVENYQKFQRAGMTLDWKAVQDIIRLLLFNFRSAAKTKLTADGLVTKLGECSSKWSKSALQVMHQLWTEHGSLVQTHQELQAVASIGQLVDIQWKLGMAVSSDTCRSLNSPYISVLMKIADTSGEISYKSFEMTVLQFQNFYRQFKEIASVLETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3TGE4</accession>
    <name>E3TGE4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10 12">Phosphatidylethanolamine-binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PEBP1</name>
        <name evidence="12" type="synonym">pebp1</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphatidylethanolamine-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="GU589430">
        <property type="protein sequence ID" value="ADO29380.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187975.1">
        <property type="nucleotide sequence ID" value="NM_001201046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TGE4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026697"/>
    <dbReference type="MEROPS" id="I51.002"/>
    <dbReference type="GeneID" id="100528776"/>
    <dbReference type="KEGG" id="ipu:100528776"/>
    <dbReference type="CTD" id="5037"/>
    <dbReference type="GeneTree" id="ENSGT00940000157251"/>
    <dbReference type="OMA" id="QEVICYE"/>
    <dbReference type="OrthoDB" id="2506647at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.280.10:FF:000003">
        <property type="entry name" value="phosphatidylethanolamine-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF147">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0722">Serine protease inhibitor</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADO29380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="NP_001187975.1"/>
        </source>
    </evidence>
    <sequence length="187" mass="20975" checksum="530B183161711389" modified="2011-01-11" version="1">MPVDIEQWTGPLSLTEVEEKPSKPLTVKYGSVEIDELGKVLTPTKVQNRPTVIEWEGCDSSKLYTLAMTDPDAPSRKDPKFREWHHFLVVNVKGNDVSSGCVMSDYVGSGPPKGTGLHRYVWLVYEQSGTVNCTERVLTNRSGDNRGKFKIQNFRKKYGLGAPVAGTCYQAEWDDYVPKLYEQLAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-09-21" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>F8TVS0</accession>
    <name>F8TVS0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cathepsin D</fullName>
            <ecNumber evidence="5">3.4.23.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">ctsd</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 24" key="1">
        <citation type="journal article" date="2011" name="Fish Shellfish Immunol." volume="31" first="164" last="169">
            <title>Molecular characterization and expression analysis of the channel catfish cathepsin D genes.</title>
            <authorList>
                <person name="Feng T."/>
                <person name="Zhang H."/>
                <person name="Liu H."/>
                <person name="Zhou Z."/>
                <person name="Niu D."/>
                <person name="Wong L."/>
                <person name="Kucuktas H."/>
                <person name="Liu X."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="21558007"/>
            <dbReference type="DOI" id="10.1016/j.fsi.2011.04.006"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="journal article" date="2016" name="Biol. Reprod." volume="95" first="19" last="19">
            <title>Transcriptome Display During Testicular Differentiation of Channel Catfish (Ictalurus punctatus) as Revealed by RNA-Seq Analysis.</title>
            <authorList>
                <person name="Zeng Q."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Zhang Y."/>
                <person name="Yuan Z."/>
                <person name="Jiang C."/>
                <person name="Chen A."/>
                <person name="Fu Q."/>
                <person name="Su B."/>
                <person name="Dunham R."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="27307075"/>
            <dbReference type="DOI" id="10.1095/biolreprod.116.138818"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="23" key="4">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="5">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Acid protease active in intracellular protein breakdown.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Specificity similar to, but narrower than, that of pepsin A. Does not cleave the 4-Gln-|-His-5 bond in B chain of insulin.</text>
            <dbReference type="EC" id="3.4.23.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.5" evidence="5"/>
    <dbReference type="EMBL" id="JF415913">
        <property type="protein sequence ID" value="AEC33270.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001244039.1">
        <property type="nucleotide sequence ID" value="NM_001257110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="F8TVS0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027058"/>
    <dbReference type="MEROPS" id="A01.009"/>
    <dbReference type="GeneID" id="100862736"/>
    <dbReference type="KEGG" id="ipu:100862736"/>
    <dbReference type="CTD" id="1509"/>
    <dbReference type="GeneTree" id="ENSGT00940000155733"/>
    <dbReference type="OMA" id="KYDHDAS"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05490">
        <property type="entry name" value="Cathepsin_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000009">
        <property type="entry name" value="Aspartic proteinase A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1960:FF:000001">
        <property type="entry name" value="Cathepsin D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000066">
        <property type="entry name" value="Napsin A aspartic peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012848">
        <property type="entry name" value="Aspartic_peptidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033144">
        <property type="entry name" value="Cathepsin_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF42">
        <property type="entry name" value="CATHEPSIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07966">
        <property type="entry name" value="A1_Propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9 19" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="12 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0458">Lysosome</keyword>
    <keyword evidence="7 19" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 20 24" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5011323964" description="Cathepsin D" evidence="20 24">
        <location>
            <begin position="19"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="21">
        <location>
            <begin position="76"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="18">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="18">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="107"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="272"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="315"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00055742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR633144-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="AEC33270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="NP_001244039.1"/>
        </source>
    </evidence>
    <sequence length="396" mass="43100" checksum="48C198EADDBB66CC" modified="2011-09-21" version="1">MKLACLLLLVLAAWTADAVVRIPLTKFRSIRRSMSDSGRSVEELLANSRHMKYNLGFPPKSGPTPETLKNYLDAQYYGEIGLGSPVQTFTVVFDTGSSNLWVPSVHCSLTDIACLLHHKYNGAKSSTYVKNGTAFAIQYGSGSLSGYLSQDVCTIGDIAVEKQIFGEAIKQPGVAFIAAKFDGILGMAYPRIAVDGVPPVFDMMMSQKKVEKNVFSFYLNRNPDTQPGGELLLGGTDPKFYTGDFHYVNITRQAYWQIHMDGMTIGSQLTLCKGGCEAIVDTGTSLITGPAAEVKALQKAIGAIPLIQGEYMVDCKKVPSLPTISFNLGGQTYTLTGEQYILKESQAGREICLSGFMALDIPPPAGPLWILGDVFIGQYYTMFDRENNRVGFAKAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5U7A3</accession>
    <name>W5U7A3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIPF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Yipf1</name>
        <name evidence="11" type="synonym">yipf1</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">cis-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT406968">
        <property type="protein sequence ID" value="AHH37803.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017346692.1">
        <property type="nucleotide sequence ID" value="XM_017491203.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U7A3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000014361"/>
    <dbReference type="GeneID" id="108278062"/>
    <dbReference type="KEGG" id="ipu:108278062"/>
    <dbReference type="CTD" id="54432"/>
    <dbReference type="GeneTree" id="ENSGT00390000010157"/>
    <dbReference type="OMA" id="KRAAGNY"/>
    <dbReference type="OrthoDB" id="10256463at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039765">
        <property type="entry name" value="Yip5/YIPF1/YIPF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822:SF4">
        <property type="entry name" value="PROTEIN YIPF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="8">
        <location>
            <begin position="101"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="26"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AHH37803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017346692.1"/>
        </source>
    </evidence>
    <sequence length="303" mass="33983" checksum="78FA744172E5FA0D" modified="2014-04-16" version="1">MANTDFLLQFQDFGDDAGVTHRSDGATAVSMDDEPFGKSPTQRRAAGSEDHLPGGNDQTELLGSERKSAAFWTLEFYQTFFNVDTYQVRDRIISSVLPWRGKNFVRLHIRNNPDLYGPFWICATLVFAIGVSGNMSSFLVHRGESHYKYVPEFRKVTMAATAIYSYAWLVPLALWGFLTWRSRKICSTLSYSFLEIVCVYGYSLSVYIPAVVLWVVPNEALRWSSIVVALCLSGSVLVLTFWPVMRDDRPRVVLAVLSAVVTLHVLLAIGCKMCFFSTYESETAQKPAAETNVAQNLTATKTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5U8H9</accession>
    <name>W5U8H9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MICOS complex subunit MIC60</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Mitofilin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Immt</name>
        <name evidence="12" type="synonym">immt</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion inner membrane</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the MICOS complex subunit Mic60 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT407130">
        <property type="protein sequence ID" value="AHH37869.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053538402.1">
        <property type="nucleotide sequence ID" value="XM_053682427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U8H9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019767"/>
    <dbReference type="GeneID" id="108268968"/>
    <dbReference type="KEGG" id="ipu:108268968"/>
    <dbReference type="GeneTree" id="ENSGT00390000002313"/>
    <dbReference type="OMA" id="WIKFREL"/>
    <dbReference type="OrthoDB" id="10261039at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019133">
        <property type="entry name" value="MIC60"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15415:SF7">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15415">
        <property type="entry name" value="MITOFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09731">
        <property type="entry name" value="Mitofilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="110"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="168"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="302"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="407"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="181"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AHH37869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053538402.1"/>
        </source>
    </evidence>
    <sequence length="765" mass="84554" checksum="3552A57EB29BB44A" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5U8Q9</accession>
    <name>W5U8Q9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sedoheptulokinase</fullName>
            <ecNumber evidence="10">2.7.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Carbohydrate kinase-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">Shpk</name>
        <name evidence="16" type="synonym">shpk</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as a modulator of macrophage activation through control of glucose metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>sedoheptulose + ATP = D-sedoheptulose 7-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23844"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16802"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.14" evidence="10"/>
    <dbReference type="EMBL" id="JT408511">
        <property type="protein sequence ID" value="AHH38361.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017315463.1">
        <property type="nucleotide sequence ID" value="XM_017459974.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U8Q9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019997"/>
    <dbReference type="GeneID" id="108260044"/>
    <dbReference type="KEGG" id="ipu:108260044"/>
    <dbReference type="CTD" id="23729"/>
    <dbReference type="GeneTree" id="ENSGT01000000214434"/>
    <dbReference type="OMA" id="TWQDTRC"/>
    <dbReference type="OrthoDB" id="10264182at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050277">
        <property type="term" value="F:sedoheptulokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07777">
        <property type="entry name" value="ASKHA_NBD_FGGY_SHK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000111">
        <property type="entry name" value="Sedoheptulokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000132">
        <property type="entry name" value="Sedoheptulokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF67">
        <property type="entry name" value="SEDOHEPTULOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="13">
        <location>
            <begin position="11"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00052736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00057196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00066341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00069425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AHH38361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017315463.1"/>
        </source>
    </evidence>
    <sequence length="473" mass="50838" checksum="B5A4BCDB0A19496B" modified="2014-04-16" version="1">MAALRGSSAKYVLGIDLGTTSIKVVLFDTRSKTVTDSCSLQTNSDVIDDMHEHVKEQDPVRIIAALDQCMCALPKEKLHDVICIGVSGQMHGVLFWKSNTACEWYSSHGSHKFRPQDVSHLITWQDGRCTSDFLSSLPKPESHLNVATGFGCASILWYTKHSPECLSKFSVSGTIQDYVVSMLCGLEVCLMSAQNAASWGYFNTTNNQWNTEILQGAGFPVHLLPTLVDSGSIAGQTCCEWHGIPANTPVGAALGDFQCSVYSCMTDKTDAVLNMSTSAQLTFSMPADFSPGNVPDACSPIAYFPYFHNSYLAVAAALNGGNVLAAFVGMLTCWMKDFGVEVSDSEVYSKLIECALTQPSTDLRVTPTLLGERHDPSSLAVVSHISPTNLSLAHVTRAVCAGIVANLAAMMPPHVLLGAGVKRIMGSGSALSRNEALRQEVEQTFPFPVVYGQDVDSAVGVAMVFNDKLLGND</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>W5UAN7</accession>
    <name>W5UAN7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9 11">Adaptin ear-binding coat-associated protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Necap2</name>
        <name evidence="11" type="synonym">necap2</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in endocytosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Clathrin-coated vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NECAP family.</text>
    </comment>
    <dbReference type="EMBL" id="JT410524">
        <property type="protein sequence ID" value="AHH39101.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053530099.1">
        <property type="nucleotide sequence ID" value="XM_053674124.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UAN7"/>
    <dbReference type="GeneID" id="108254698"/>
    <dbReference type="KEGG" id="ipu:108254698"/>
    <dbReference type="GeneTree" id="ENSGT00390000009359"/>
    <dbReference type="OrthoDB" id="10265489at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13228">
        <property type="entry name" value="PHear_NECAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000064">
        <property type="entry name" value="Adaptin ear-binding coat-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012466">
        <property type="entry name" value="NECAP_PHear"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12847:SF16">
        <property type="entry name" value="ADAPTIN EAR-BINDING COAT-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12847">
        <property type="entry name" value="ATP-BINDING CASSETTE ABC TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07933">
        <property type="entry name" value="DUF1681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NECAP PHear" evidence="8">
        <location>
            <begin position="6"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="167"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="206"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AHH39101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053530099.1"/>
        </source>
    </evidence>
    <sequence length="255" mass="27340" checksum="3462C4A9CFCDB373" modified="2014-04-16" version="1">MADDAYESILCVKPEVHVYKIPPRASNRGYRAADWKLDEPSWTGRMKITAKAKIAFIKLEDRNTGELFAQAPVDQYPGASVEAVTDSSRYFVVKIEDGNGRHAFIGLGFADRGDSFDFNVALQDHFKWVKQEGELAKQEANQSTAPKLDLGFKEGQTIKISLGNIKKKDTAASKSRPAGAGGLLPPPPGGKSGGLCSPPPSGSAHQIATPTQQSTGTLLDFGAPQTQASQDQWGDFTAAGSGTTQESVKPGWVQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5UC57</accession>
    <name>W5UC57_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pyruvate dehydrogenase phosphatase regulatory subunit, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PDPR</name>
        <name evidence="14" type="synonym">pdpr</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Decreases the sensitivity of PDP1 to magnesium ions, and this inhibition is reversed by the polyamine spermine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer of a catalytic (PDP1) and a regulatory (PDPR) subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GcvT family.</text>
    </comment>
    <dbReference type="EMBL" id="JT411673">
        <property type="protein sequence ID" value="AHH39548.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JT417101">
        <property type="protein sequence ID" value="AHH42256.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017322139.1">
        <property type="nucleotide sequence ID" value="XM_017466650.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UC57"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000010849"/>
    <dbReference type="GeneID" id="108264785"/>
    <dbReference type="KEGG" id="ipu:108264785"/>
    <dbReference type="CTD" id="55066"/>
    <dbReference type="GeneTree" id="ENSGT00940000159082"/>
    <dbReference type="OMA" id="TKFPDRE"/>
    <dbReference type="OrthoDB" id="429143at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.120:FF:000046">
        <property type="entry name" value="Pyruvate dehydrogenase phosphatase regulatory mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1400:FF:000003">
        <property type="entry name" value="Pyruvate dehydrogenase phosphatase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.110:FF:000004">
        <property type="entry name" value="Pyruvate dehydrogenase phosphatase regulatory subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.110">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1400">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032503">
        <property type="entry name" value="FAO_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028896">
        <property type="entry name" value="GcvT/YgfZ/DmdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029043">
        <property type="entry name" value="GcvT/YgfZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013977">
        <property type="entry name" value="GcvT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006222">
        <property type="entry name" value="GCVT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757:SF15">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE PHOSPHATASE REGULATORY SUBUNIT, MITOCHONDRIAL-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16350">
        <property type="entry name" value="FAO_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01571">
        <property type="entry name" value="GCV_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08669">
        <property type="entry name" value="GCV_T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101790">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103025">
        <property type="entry name" value="Folate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12 14" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="8">
        <location>
            <begin position="58"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="FAD dependent oxidoreductase central" evidence="11">
        <location>
            <begin position="420"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="GCVT N-terminal" evidence="9">
        <location>
            <begin position="476"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="domain" description="Aminomethyltransferase C-terminal" evidence="10">
        <location>
            <begin position="779"/>
            <end position="871"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AHH39548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017322139.1"/>
        </source>
    </evidence>
    <sequence length="893" mass="100680" checksum="109CA012BA1F4300" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5UDG9</accession>
    <name>W5UDG9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5 7">26S proteasome non-ATPase regulatory subunit 11B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">psmd11b</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the lid subcomplex of the 19S proteasome regulatory particle complex (also named PA700 complex). The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S9 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT412600">
        <property type="protein sequence ID" value="AHH39915.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017339532.1">
        <property type="nucleotide sequence ID" value="XM_017484043.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UDG9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027574"/>
    <dbReference type="GeneID" id="108274134"/>
    <dbReference type="KEGG" id="ipu:108274134"/>
    <dbReference type="CTD" id="322265"/>
    <dbReference type="GeneTree" id="ENSGT00530000063301"/>
    <dbReference type="OrthoDB" id="1418352at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.570:FF:000003">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050871">
        <property type="entry name" value="26S_Proteasome/COP9_Components"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040780">
        <property type="entry name" value="Rpn6_C_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040773">
        <property type="entry name" value="Rpn6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10678">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 11/COP9 SIGNALOSOME COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18503">
        <property type="entry name" value="RPN6_C_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18055">
        <property type="entry name" value="RPN6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2 5 7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="4">
        <location>
            <begin position="228"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00062507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AHH39915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017339532.1"/>
        </source>
    </evidence>
    <sequence length="422" mass="47458" checksum="F7DDAEC5F0350D8A" modified="2014-04-16" version="1">MAAAAVVEFQRAQSLISTDRDASIDILHSIVRRDVQQDDEEAVRVKEQSILELGTLLAKTGQAAELGGLLKFVRPFLISISKAKAARLVRSLLDLFLDMEAATGQEVELCLECIEWAKAEKRTFLRQALEARLISLYFDTKRYQEALQLGSQLLQELKKMDDKALLVEVQLLESKTYHALSNLPKARAALTSARTTANAIYCPPKLQAALDMQSGIIHAAEEKDWKTAYSYFFEAFEGYDSIDSPRAITALKYMLLCKIMLNLPEEVQALISGKLALRYAGRQTDALKCVAQASKNRSLADFEKALTEYTKELRDDPIISTHLAKLYDNLLEQNLIRVIEPFSRVQITHISDLIKLSKGDVERKLSQMILDKKFHGILDQGEGVLIIFEEPPVDKTYEAALETIQNMSKVVDSLYNKAKKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5UFE6</accession>
    <name>W5UFE6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cysteine desulfurase</fullName>
            <ecNumber evidence="3">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Nfs1</name>
        <name evidence="17" type="synonym">nfs1</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-cysteinyl-[cysteine desulfurase] + L-cysteine = S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:17457"/>
            <dbReference type="Rhea" id="RHEA-COMP:12157"/>
            <dbReference type="Rhea" id="RHEA-COMP:12158"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer. Component of the mitochondrial core iron-sulfur cluster (ISC) complex composed of NFS1, LYRM4, NDUFAB1, ISCU, FXN, and FDX2; this complex is a heterohexamer containing two copies of each monomer. Component of cyteine desulfurase complex composed of NFS1, LYRM4 and NDUFAB1; this complex contributes to the activation of cysteine desulfurase activity and NFS1 stabilization. Interacts (homodimer form) with ISCU (D-state); each monomer interacts with the C-terminal regions of each NFS1 monomer. Interacts with HSPA9. Interacts (via homodimer form) with FDX2. Interacts (via homodimer form) with FXN. Interacts with LYRM4. Component of a complex composed of FXN, NFS1, LYRM4 and ISCU.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="3"/>
    <dbReference type="EMBL" id="JT408594">
        <property type="protein sequence ID" value="AHH38397.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053530033.1">
        <property type="nucleotide sequence ID" value="XM_053674058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UFE6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000036779"/>
    <dbReference type="GeneID" id="108255141"/>
    <dbReference type="KEGG" id="ipu:108255141"/>
    <dbReference type="OMA" id="KGLYWAR"/>
    <dbReference type="OrthoDB" id="10250117at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099128">
        <property type="term" value="C:mitochondrial [2Fe-2S] assembly complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044572">
        <property type="term" value="P:[4Fe-4S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000003">
        <property type="entry name" value="Cysteine desulfurase IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000002">
        <property type="entry name" value="Cysteine desulfurase IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00331">
        <property type="entry name" value="Cys_desulf_IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010240">
        <property type="entry name" value="Cys_deSase_IscS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02006">
        <property type="entry name" value="IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002806">
        <property type="entry name" value="PRK02948.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010611">
        <property type="entry name" value="PRK14012.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601:SF34">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601">
        <property type="entry name" value="CYSTEINE DESULFURYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="14">
        <location>
            <begin position="55"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AHH38397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_053530033.1"/>
        </source>
    </evidence>
    <sequence length="452" mass="50111" checksum="94A9BF4901D3E380" modified="2014-04-16" version="1">MLSKSVVRTCLGCVRGVSSSSFRLNSPESSVSASLTHTEGIKRRELEKNEMRPLYMDFQATTPMDPRVLDAMLPYQVNYYGNPHSRTHAYGWESESAVERARKQVADLIGADPREIIFTSGATESNNMSIKGVAHFYKAKKKHIITTQTEHKCVLDSCRVLEAEGFKVTYLPVKNNGLIDLQQLEETIRSDTSLVSVMTVNNEIGVKQPIKEIGQICRSKNVFFHTDAAQAIGKIPINVSTLKVDLMSISGHKLYGPKGVGALYVRRRPRVRLEPLQSGGGQERGLRSGTVPTPLAVGLGTACEIAQQEMEYDHARVSMLANQLVQKIMSEIPDVVMNGDPVQRYPGCLNLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTEEEVDYTAEKCIQHVQRLREMSPLWEMVQEGIDLKSIKWTQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5UFY7</accession>
    <name>W5UFY7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5 7 8">Protein Tob2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TOB2</name>
        <name evidence="7 8 9" type="synonym">LOC108272833</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7 8 9" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7 8 9">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BTG family.</text>
    </comment>
    <dbReference type="EMBL" id="JT414922">
        <property type="protein sequence ID" value="AHH40810.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017337106.1">
        <property type="nucleotide sequence ID" value="XM_017481617.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017337107.1">
        <property type="nucleotide sequence ID" value="XM_017481618.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017337108.1">
        <property type="nucleotide sequence ID" value="XM_017481619.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UFY7"/>
    <dbReference type="GeneID" id="108272833"/>
    <dbReference type="KEGG" id="ipu:108272833"/>
    <dbReference type="GeneTree" id="ENSGT00940000154208"/>
    <dbReference type="OrthoDB" id="19928at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.90:FF:000001">
        <property type="entry name" value="TOB1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.90">
        <property type="entry name" value="Anti-proliferative protein, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002087">
        <property type="entry name" value="Anti_prolifrtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036054">
        <property type="entry name" value="BTG-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009818">
        <property type="entry name" value="PAM2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015676">
        <property type="entry name" value="Tob1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17537:SF3">
        <property type="entry name" value="PROTEIN TOB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17537">
        <property type="entry name" value="TRANSDUCER OF ERBB2 TOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07742">
        <property type="entry name" value="BTG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07145">
        <property type="entry name" value="PAM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00310">
        <property type="entry name" value="ANTIPRLFBTG1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00099">
        <property type="entry name" value="btg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160696">
        <property type="entry name" value="BTG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01203">
        <property type="entry name" value="BTG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Anti-proliferative protein" evidence="4">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="181"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="202"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AHH40810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017337106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017337107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017337108.1"/>
        </source>
    </evidence>
    <sequence length="394" mass="41807" checksum="A98AEF0E25D9C10A" modified="2014-04-16" version="1">MHLEVKVALNFIVSYLYNKLPRRRADLFGEELERILVSRFEGHWYPEAPLRGSAFRCLHLGVPGDPVVDLAARRSGLDMEEVRANVPPELSIWIDPYEVSYQIGEKGAVKVLYMEDPPGLGGEPEVSECLGGLCKGDVELEDGKNLGFNPDAQVFVPIGGQASPVMLPSLSNSPTPLCQGLFSYAGSSTPTDPTVHSNTSSPSPPSTGMPYPHPAAQPSSRPTPQQITFTTASFAATKFGSTKMKKCMGNGVVGGSGSVSAPQQRMLSHSPTAMSSISPPGLVKHKPLSLSMHSLAGPVSSQLSPNAKEFVYPASQGPLYFEAEAPHLTHTGPFQTPLPGPAHPHATFDPFSSQPPGHAMGVMGSGSGISFMEGLGGYNLQYSSQAFQPVVLAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5UIP6</accession>
    <name>W5UIP6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5 7">Cytochrome P450 20A1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CYP20A1</name>
        <name evidence="7" type="synonym">LOC108272693</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT411689">
        <property type="protein sequence ID" value="AHH39557.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017336792.1">
        <property type="nucleotide sequence ID" value="XM_017481303.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UIP6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029019"/>
    <dbReference type="GeneID" id="108272693"/>
    <dbReference type="KEGG" id="ipu:108272693"/>
    <dbReference type="OMA" id="YFQVWSE"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052666">
        <property type="entry name" value="CYP450_20A1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24280">
        <property type="entry name" value="CYTOCHROME P450 20A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24280:SF4">
        <property type="entry name" value="CYTOCHROME P450 20A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="2 3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="3">
        <location>
            <position position="410"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AHH39557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017336792.1"/>
        </source>
    </evidence>
    <sequence length="463" mass="51645" checksum="7A68AD6C646AD0BF" modified="2014-04-16" version="1">MLDFAIFAVTFVVILVGAVLYLYPSSRRASGVPGLNPTEEKDGNLQDIVNKGSLHEFLVGLHDRFGPVASFWFGRRPVVSLGSVDELRQHINPNWTTDSFETILKSLLGYQSGAGVGGAEALMRKKVYEGAINKTLENNFPLLLKLVEELVEKWQSDPKSQHTPICAHLLGLAMKAVTQLAMGSRFADDSEVIRFRKNHEAIWSEIGKGYLDGSMEKSSSRKAHYESALSEMESMLKAVIKERSGRGSSQSAFMDSLLQAKLSERQVMEESMVFTLAGSVITANLCIWAVHFLSLSEAVQEKLQEELAEVLGDEPVSLDKIPQLRYCQQVLNETVRTAKLTPIAARLQEVEGKVDGHVIPKETLVIYALGVVLQDAETWTLPYRFNPDRFTDETVMKSFSLLGFSGNRACPELRFAYTVAAVLLSSLVRRLRLQRLDGQVVESRYELVTTPKDDTWITVSKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5UJH7</accession>
    <name>W5UJH7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DCC-interacting protein 13-alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Adapter protein containing PH domain, PTB domain and leucine zipper motif 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">APPL1</name>
        <name evidence="21" type="synonym">appl1</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Ruffle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Phagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT417209">
        <property type="protein sequence ID" value="AHH42299.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017305770.1">
        <property type="nucleotide sequence ID" value="XM_017450281.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UJH7"/>
    <dbReference type="GeneID" id="108254880"/>
    <dbReference type="KEGG" id="ipu:108254880"/>
    <dbReference type="CTD" id="26060"/>
    <dbReference type="OrthoDB" id="10070851at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071363">
        <property type="term" value="P:cellular response to growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009891">
        <property type="term" value="P:positive regulation of biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051050">
        <property type="term" value="P:positive regulation of transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023052">
        <property type="term" value="P:signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13247">
        <property type="entry name" value="BAR-PH_APPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13158">
        <property type="entry name" value="PTB_APPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000034">
        <property type="entry name" value="DCC-interacting protein 13-alpha isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000178">
        <property type="entry name" value="DCC-interacting protein 13-alpha isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000067">
        <property type="entry name" value="Putative DCC-interacting protein 13-beta isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047181">
        <property type="entry name" value="DP13A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047236">
        <property type="entry name" value="PH_DP13A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047237">
        <property type="entry name" value="PTB_APPL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46415">
        <property type="entry name" value="ADAPTOR PROTEIN, PHOSPHOTYROSINE INTERACTION, PH DOMAIN AND LEUCINE ZIPPER-CONTAINING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46415:SF3">
        <property type="entry name" value="DCC-INTERACTING PROTEIN 13-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="18">
        <location>
            <begin position="277"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="PID" evidence="17">
        <location>
            <begin position="502"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="397"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="460"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="632"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="424"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="632"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="700"/>
            <end position="710"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00070936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00076400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AHH42299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017305770.1"/>
        </source>
    </evidence>
    <sequence length="710" mass="80045" checksum="29B994CD8F958E7D" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5UKN0</accession>
    <name>W5UKN0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">PHD finger protein 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20 22" type="primary">phf23b</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Acts as a negative regulator of autophagy.</text>
    </comment>
    <comment type="function">
        <text evidence="16">Modulates chromatin structure and DNA damage response by regulating key determinants of chromatin compaction and DNA damage response. Binds H3K4me3-containing chromatin and promotes DNA condensation by recruiting corepressors such as TRIM28 and H3K9 methyltransferase SETDB1. Required for normal chromosome condensation during the early stages of mitosis. Required for normal chromosome separation during mitosis. Increases both chromatin-associated levels and activity of H3K9 methyltransferases, such as SETDB1, thus enhancing H3K9 trimethylation. Essential for testicular stem-cell differentiation and sustained spermatogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the PHF23 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT418414">
        <property type="protein sequence ID" value="AHH42729.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017328275.1">
        <property type="nucleotide sequence ID" value="XM_017472786.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UKN0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007800"/>
    <dbReference type="GeneID" id="108268089"/>
    <dbReference type="KEGG" id="ipu:108268089"/>
    <dbReference type="CTD" id="100004597"/>
    <dbReference type="GeneTree" id="ENSGT00530000063882"/>
    <dbReference type="OMA" id="CRDMRRS"/>
    <dbReference type="OrthoDB" id="79252at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902902">
        <property type="term" value="P:negative regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901097">
        <property type="term" value="P:negative regulation of autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031398">
        <property type="term" value="P:positive regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15631">
        <property type="entry name" value="PHD_PHF23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000039">
        <property type="entry name" value="PHD finger protein 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14571">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SET-26-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14571:SF8">
        <property type="entry name" value="PHD FINGER PROTEIN 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13831">
        <property type="entry name" value="PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="10" id="KW-0072">Autophagy</keyword>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger PHD-type" evidence="19">
        <location>
            <begin position="261"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="49"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="132"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="56"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="151"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="222"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00055120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00068751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="AHH42729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017328275.1"/>
        </source>
    </evidence>
    <sequence length="316" mass="34837" checksum="206280E5D916A588" modified="2014-04-16" version="1">MLEIMSEHQDSLLKRKAEPLPPERRKRTVEDFNKFCSFVLAYAGYIPSPKEERPWSPDSSSSPHSSGTSGEGGVGGGASSNMSASWGDGNSDLHTIHTFVQKARANKSVKTMQRLPSDNTLLDKMRLKDSLYKGQASSKAERKKDKKLKRLSLGSGAGDSSSSSGEKRARIKRSKNSKQPKSLKKLKSSALSETDSETGLGHGEEHAGQPRGLQSQRPNLVKSEHDEASRDAEMSSSEGETWIADEDIMVESGDDSWDLITCYCGKPFAGRPMIECNQCSIWVHLSCAKIKKSNVPDIFYCHKCREARRSGHKKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5ULG5</accession>
    <name>W5ULG5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CXXC-type zinc finger protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">CpG-binding protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">PHD finger and CXXC domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">CXXC1</name>
        <name evidence="21" type="synonym">cxxc1a</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Transcriptional activator that exhibits a unique DNA binding specificity for CpG unmethylated motifs with a preference for CpGG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT419224">
        <property type="protein sequence ID" value="AHH42999.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017324314.1">
        <property type="nucleotide sequence ID" value="XM_017468825.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ULG5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018626"/>
    <dbReference type="GeneID" id="108265945"/>
    <dbReference type="KEGG" id="ipu:108265945"/>
    <dbReference type="CTD" id="393571"/>
    <dbReference type="OMA" id="PMDTMSA"/>
    <dbReference type="OrthoDB" id="419183at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15553">
        <property type="entry name" value="PHD_Cfp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000138">
        <property type="entry name" value="CXXC-type zinc finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022056">
        <property type="entry name" value="CpG-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037869">
        <property type="entry name" value="Spp1/CFP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002857">
        <property type="entry name" value="Znf_CXXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46174">
        <property type="entry name" value="CXXC-TYPE ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46174:SF5">
        <property type="entry name" value="CXXC-TYPE ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12269">
        <property type="entry name" value="CpG_bind_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02008">
        <property type="entry name" value="zf-CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51058">
        <property type="entry name" value="ZF_CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="17">
        <location>
            <begin position="24"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="CXXC-type" evidence="18">
        <location>
            <begin position="118"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="82"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="226"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="346"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="92"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="236"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="250"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="261"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="275"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00079319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AHH42999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017324314.1"/>
        </source>
    </evidence>
    <sequence length="571" mass="67081" checksum="216BD71BC8032BBA" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5USJ6</accession>
    <name>W5USJ6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3 5">Myc target protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">myct1</name>
        <name evidence="5" type="synonym">myct1a</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT418062">
        <property type="protein sequence ID" value="AHH42603.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017311274.1">
        <property type="nucleotide sequence ID" value="XM_017455785.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5USJ6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029087"/>
    <dbReference type="GeneID" id="108257773"/>
    <dbReference type="KEGG" id="ipu:108257773"/>
    <dbReference type="CTD" id="796556"/>
    <dbReference type="GeneTree" id="ENSGT00390000011642"/>
    <dbReference type="OMA" id="SFWGNNG"/>
    <dbReference type="OrthoDB" id="9943706at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035162">
        <property type="term" value="P:embryonic hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029180">
        <property type="entry name" value="Myc_target_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14869">
        <property type="entry name" value="MYC TARGET PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14869:SF0">
        <property type="entry name" value="MYC TARGET PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15179">
        <property type="entry name" value="Myc_target_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="158"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AHH42603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017311274.1"/>
        </source>
    </evidence>
    <sequence length="220" mass="23913" checksum="AEE1164939ABA0E2" modified="2014-04-16" version="1">MYISAPFFSVSPDGAMADNSTHPILEILQSFSTRDVILAFCLSMVVGLLLGALVYVVLTWMARRRASATITRMPAPRSSGRPSSPRTRPVFSRQSSAYDRRSNNSLASAAFSFHRQTTSSPVDHADLLGRKDSFRASTFHPLIQCSQIAREAEEGNHGTLTSSAGATGTNSTASSMVTPPRPRPRPDSFWGNNSLRSLSVTQTPPPAYDSIIRAYQETST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PHZ0</accession>
    <name>A0A2D0PHZ0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Msx2-interacting protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">SMART/HDAC1-associated repressor protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">SPEN homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">si:ch1073-335m2.2</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM Spen family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017305444.1">
        <property type="nucleotide sequence ID" value="XM_017449955.3"/>
    </dbReference>
    <dbReference type="GeneID" id="108254705"/>
    <dbReference type="OrthoDB" id="6407164at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <sequence length="3241" mass="359684" checksum="A9CDCBA3CA27574A" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PJC4</accession>
    <name>A0A2D0PJC4_ICTPU</name>
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        <submittedName>
            <fullName evidence="11">Synaptoporin isoform X1</fullName>
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        <name evidence="11" type="primary">synpra</name>
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    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptophysin/synaptobrevin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017306087.1">
        <property type="nucleotide sequence ID" value="XM_017450598.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PJC4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034993"/>
    <dbReference type="GeneID" id="108255022"/>
    <dbReference type="KEGG" id="ipu:108255022"/>
    <dbReference type="CTD" id="558290"/>
    <dbReference type="OrthoDB" id="10006326at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001285">
        <property type="entry name" value="Synaptophysin/porin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10306">
        <property type="entry name" value="SYNAPTOPHYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10306:SF16">
        <property type="entry name" value="SYNAPTOPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00220">
        <property type="entry name" value="SYNAPTOPHYSN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="200"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="9">
        <location>
            <begin position="14"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017306087.1"/>
        </source>
    </evidence>
    <sequence length="289" mass="32078" checksum="271E2900A7F5380C" modified="2017-12-20" version="1">MEPANKVSTGTFQVLKLPLGFIRLLEWLFAIFAFATCGGYTGQLRVSVDCVNKTDSNLSITIKFAYPFRLNQVTFNVPTCEGRQQEELHLDGDFSSSAEFFVTIAVFAFLYSLFATVVYIFYQNKYRENNRGPFIDFIVTVVFSFMWLVGSSAWAKALSDVKVATDPDEVLLLTTACKVQTNKCGSVYGPVWSGLNTSVVFGYLNFVLWAGNIWFVFKETGWHKGGAARYPTPAPEKHTTAFNQQPYNQQNYNQGTFDQSGASFGGTGGFGQSDYSQVGSYTSSGLTSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PNT1</accession>
    <name>A0A2D0PNT1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Neuropeptide FF receptor 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">G-protein coupled receptor 74</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">npffr2a</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017306730.1">
        <property type="nucleotide sequence ID" value="XM_017451241.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PNT1"/>
    <dbReference type="GeneID" id="108255334"/>
    <dbReference type="KEGG" id="ipu:108255334"/>
    <dbReference type="CTD" id="560955"/>
    <dbReference type="OrthoDB" id="5953793at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008188">
        <property type="term" value="F:neuropeptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031628">
        <property type="term" value="F:opioid receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043408">
        <property type="term" value="P:regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15980">
        <property type="entry name" value="7tmA_NPFFR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000137">
        <property type="entry name" value="neuropeptide FF receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005395">
        <property type="entry name" value="NPFF_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005397">
        <property type="entry name" value="NPFF_rcpt_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241:SF132">
        <property type="entry name" value="NEUROPEPTIDE FF RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241">
        <property type="entry name" value="NEUROPEPTIDE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01570">
        <property type="entry name" value="NPFFRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01572">
        <property type="entry name" value="NPFFRECEPTR2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 14 19" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 14 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="334"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="84"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="389"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="389"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017306730.1"/>
        </source>
    </evidence>
    <sequence length="442" mass="50521" checksum="26FFE41C084DB9D5" modified="2017-12-20" version="1">MMQLLHWLILIKKKRGKKRKRSKMDGELNITLVDDENWTLPNISREHTIRRRNITYVGFYLHETSVAAIFIVSYLLIFLVCMVGNGVVCFIVLRSKTMRTVTNLFILNLAVSDLLVGIFCMPTTLLDNIITGWPFGSMVCKMSGMVQGISVSASVFTLVAIAVDRFRCIVYPFKQKLTISTATLIIVIIWVLAISIMCPSGVMLQVTKEQTIRVLLGDGNRSSPFYWCRENWPNQEMRKIYTTVLFANIYLAPLSLIVIMYVRIGITLFKTAVPTGGKPGHDNRHTVSKKKQRVIKMLLVVAFLFILSWLPLWTLMMLTDYARLTENQYRLINIYIYPFAHWLAFFNSSVNPIIYGFFNENFRRGFQAMFKFNLCSVGGHRRKTYSHRMQSNSVLPANPQPSTEPISLNSVDNPRVNHVNEQDLVMEDLEKVSESSMGGGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PQ75</accession>
    <name>A0A2D0PQ75_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">KN motif and ankyrin repeat domain-containing protein 1a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">kank1a</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017307586.1">
        <property type="nucleotide sequence ID" value="XM_017452097.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000016889"/>
    <dbReference type="GeneID" id="108255828"/>
    <dbReference type="KEGG" id="ipu:108255828"/>
    <dbReference type="CTD" id="777729"/>
    <dbReference type="OrthoDB" id="5406014at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030837">
        <property type="term" value="P:negative regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000017">
        <property type="entry name" value="KN motif and ankyrin repeat domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047184">
        <property type="entry name" value="KANK1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021939">
        <property type="entry name" value="KN_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24168">
        <property type="entry name" value="KN MOTIF AND ANKYRIN REPEAT DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24168:SF19">
        <property type="entry name" value="KN MOTIF AND ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12075">
        <property type="entry name" value="KN_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="1148"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="1220"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="1253"/>
            <end position="1286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="82"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="182"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="977"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1317"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="375"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="470"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="219"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1005"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1031"/>
            <end position="1042"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017307586.1"/>
        </source>
    </evidence>
    <sequence length="1339" mass="146830" checksum="A921F771558831EF" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PSR8</accession>
    <name>A0A2D0PSR8_ICTPU</name>
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        <submittedName>
            <fullName evidence="13">Bloodthirsty-related gene family, member 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">btr02</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRIM/RBCC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017309603.1">
        <property type="nucleotide sequence ID" value="XM_017454114.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PSR8"/>
    <dbReference type="GeneID" id="108256876"/>
    <dbReference type="KEGG" id="ipu:108256876"/>
    <dbReference type="CTD" id="562439"/>
    <dbReference type="OrthoDB" id="6105938at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13733">
        <property type="entry name" value="SPRY_PRY_C-I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="28"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="11">
        <location>
            <begin position="256"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="170"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017309603.1"/>
        </source>
    </evidence>
    <sequence length="454" mass="51652" checksum="2B05E8AF8EF08E0E" modified="2017-12-20" version="1">MSTSTLICFNDDVMASTFSLLSKKHFLCSLCEDIFSNPVTTPCGHSFCKVCLHKYWSRSRTRKCPLCRKSFVSKPHLNVNRILADVTEHYRKSRLDGKTKSVSVGGLLNVAVEKSDAEVEHMIQERIQKMDKLKQSLKLLNSSCLREVQESHRVFSALLSSVEVAHKAVVAEVEEKQQEAEKRVDRLVKELEKEIADLKSGQTTPEDLLDGKEYLRKSFIHLPREMKDWSKVTLETDPCVGFTRKAVSDFIDAVRTEANKLSTAELKRLQIYTVELRLNHRTAHASLSISDDRKQVRHTGKQQEVPENPKRFDRVTNVMSKEALLSGRHYWEVEVGDKTDWDLGLARHSVNRKGKFTISPANGFWTLSLRNGNQYTANTLPLTCLPLNSKPRKVGVYLDYEEGRVSFFCPDSGIHIYTFTDKFTERLHPIFNPGKPHGSKNAAPLIIVTDCCTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PXM3</accession>
    <name>A0A2D0PXM3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">DBH-like monooxygenase protein 2 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">moxd1l</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the copper type II ascorbate-dependent monooxygenase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017311248.2">
        <property type="nucleotide sequence ID" value="XM_017455759.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PXM3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030567"/>
    <dbReference type="GeneID" id="108257752"/>
    <dbReference type="KEGG" id="ipu:108257752"/>
    <dbReference type="CTD" id="553484"/>
    <dbReference type="OrthoDB" id="10003276at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030667">
        <property type="term" value="C:secretory granule membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004500">
        <property type="term" value="F:dopamine beta-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042420">
        <property type="term" value="P:dopamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042421">
        <property type="term" value="P:norepinephrine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006589">
        <property type="term" value="P:octopamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09631">
        <property type="entry name" value="DOMON_DOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.230:FF:000001">
        <property type="entry name" value="Monooxygenase, DBH-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1210:FF:000001">
        <property type="entry name" value="Monooxygenase, DBH-like 1, like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1210">
        <property type="entry name" value="Cellobiose dehydrogenase, cytochrome domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.310">
        <property type="entry name" value="Copper type II, ascorbate-dependent monooxygenase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014784">
        <property type="entry name" value="Cu2_ascorb_mOase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000323">
        <property type="entry name" value="Cu2_ascorb_mOase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036939">
        <property type="entry name" value="Cu2_ascorb_mOase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024548">
        <property type="entry name" value="Cu2_monoox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000945">
        <property type="entry name" value="DBH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045266">
        <property type="entry name" value="DOH_DOMON"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005018">
        <property type="entry name" value="DOMON_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008977">
        <property type="entry name" value="PHM/PNGase_F_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10157:SF41">
        <property type="entry name" value="DBH-LIKE MONOOXYGENASE PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10157">
        <property type="entry name" value="DOPAMINE BETA HYDROXYLASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03712">
        <property type="entry name" value="Cu2_monoox_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01082">
        <property type="entry name" value="Cu2_monooxygen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03351">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00664">
        <property type="entry name" value="DoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49344">
        <property type="entry name" value="CBD9-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49742">
        <property type="entry name" value="PHM/PNGase F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50836">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0186">Copper</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5037893232" evidence="11">
        <location>
            <begin position="21"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="DOMON" evidence="12">
        <location>
            <begin position="37"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017311248.2"/>
        </source>
    </evidence>
    <sequence length="573" mass="63820" checksum="865F83746B2B89CD" modified="2023-02-22" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q6Z4</accession>
    <name>A0A2D0Q6Z4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">Zona pellucida sperm-binding protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC108259447</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Zona pellucida</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Cell membrane</location>
            <topology evidence="14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.</text>
    </comment>
    <comment type="PTM">
        <text evidence="14">Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the ZP domain family. ZPC subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017314463.1">
        <property type="nucleotide sequence ID" value="XM_017458974.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q6Z4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019598"/>
    <dbReference type="GeneID" id="108259447"/>
    <dbReference type="KEGG" id="ipu:108259447"/>
    <dbReference type="OrthoDB" id="8880842at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035805">
        <property type="term" value="C:egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032190">
        <property type="term" value="F:acrosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035804">
        <property type="term" value="F:structural constituent of egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035803">
        <property type="term" value="P:egg coat formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000344">
        <property type="term" value="P:positive regulation of acrosome reaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.3210:FF:000001">
        <property type="entry name" value="Zona pellucida sperm-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.4100:FF:000002">
        <property type="entry name" value="Zona pellucida sperm-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055356">
        <property type="entry name" value="ZP-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048290">
        <property type="entry name" value="ZP_chr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576:SF2">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23344">
        <property type="entry name" value="ZP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00023">
        <property type="entry name" value="ZPELLUCIDA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 14" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="12 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 14" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 14" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5025715368" description="Zona pellucida sperm-binding protein 3" evidence="14">
        <location>
            <begin position="22"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="15">
        <location>
            <begin position="125"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017314463.1"/>
        </source>
    </evidence>
    <sequence length="435" mass="46984" checksum="1B48FF0258BB9DF8" modified="2017-12-20" version="1">MAVNRVGFGVLLLLAVGLSAAQWPELTNVSAGFHPNKPGHSSLQVSARLPSQWMQSGPQSGSPAVAPLGLHLQNPSAAQDQQVMQRPVKKLTWRFPAAPQIPTPPAPVNFMRRSNAVPTQSVTARCNETAVYVEVRKDLFGTSAPLNIAALTLGGCAAKGVDVSSQVLVYESPLHGCNSKLTVTANELVYIFTLGIASVPLSSAPILRSPGAMVFIECHYPSFHNVSSSALLPAWIPYASAQAAEEHLVFSLRLMMDDWISERPSNYVFQLGELINIEASVVQFNHVPLRVFVDSCVATAVPDVNALPRYSFIENHGCLIDAKLTHSSSRFMPQTQAAKLGFQMEAFKFQQVNSSWFYITCILKATAASAPADAEHKACSFSGNRWAAAYGADQVCSCCSSSCGLRKGRELSSEQGLQLQKEVSLGPIRVLENVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q741</accession>
    <name>A0A2D0Q741_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC108259281</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017314113.1">
        <property type="nucleotide sequence ID" value="XM_017458624.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q741"/>
    <dbReference type="GeneID" id="108259281"/>
    <dbReference type="KEGG" id="ipu:108259281"/>
    <dbReference type="OrthoDB" id="8918348at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051138">
        <property type="entry name" value="PIM_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024298">
        <property type="entry name" value="Sec16_Sec23-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22984:SF11">
        <property type="entry name" value="AURORA KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22984">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12931">
        <property type="entry name" value="TPR_Sec16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="333"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017314113.1"/>
        </source>
    </evidence>
    <sequence length="605" mass="69043" checksum="962764F623AA9D81" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q9G3</accession>
    <name>A0A2D0Q9G3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Serine/arginine repetitive matrix protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">srrm3</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
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                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
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                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
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                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
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                <person name="Sonstegard T."/>
                <person name="Feng J."/>
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            <strain evidence="15">SDA103</strain>
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            <text>Na(+)(in) = Na(+)(out)</text>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
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                <person name="Jin Y."/>
                <person name="Yuan Z."/>
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                <person name="Tan S."/>
                <person name="Peatman E."/>
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                <person name="Qin Z."/>
                <person name="Dunham R."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
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    <reference evidence="14" key="2">
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="14">Blood</tissue>
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        <property type="entry name" value="CD80_C2-set"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_sub"/>
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        <property type="entry name" value="Ig_sub2"/>
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        <property type="entry name" value="Ig_V-set"/>
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        <property type="entry name" value="Surface_Rcpt/Adhesion_Mol"/>
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        <property type="entry name" value="CELL SURFACE GLYCOPROTEIN MUC18"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CELL SURFACE GLYCOPROTEIN MUC18-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="C2-set_2"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
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    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017315494.1"/>
        </source>
    </evidence>
    <sequence length="623" mass="68640" checksum="9E6209FFAA1990A7" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QD50</accession>
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        <submittedName>
            <fullName evidence="10 11">Protein spinster homolog 3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">spns3</name>
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    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
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            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
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    <reference evidence="10 11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11">Blood</tissue>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Spinster (TC 2.A.1.49) family.</text>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <dbReference type="InterPro" id="IPR044770">
        <property type="entry name" value="MFS_spinster-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505:SF67">
        <property type="entry name" value="PROTEIN SPINSTER HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505">
        <property type="entry name" value="SPINSTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="253"/>
        </location>
    </feature>
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        <location>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00024338"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017316232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053533155.1"/>
        </source>
    </evidence>
    <sequence length="525" mass="57318" checksum="E823630A5B99C808" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QDX8</accession>
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        <alternativeName>
            <fullName evidence="14">Acid beta-galactosidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">glb1</name>
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    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
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        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.</text>
            <dbReference type="EC" id="3.2.1.23"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer. May form higher multimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the glycosyl hydrolase 35 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.23" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017316499.1">
        <property type="nucleotide sequence ID" value="XM_017461010.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QDX8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000025031"/>
    <dbReference type="GeneID" id="108260601"/>
    <dbReference type="KEGG" id="ipu:108260601"/>
    <dbReference type="CTD" id="2720"/>
    <dbReference type="OMA" id="FWNIHEQ"/>
    <dbReference type="OrthoDB" id="1657402at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006689">
        <property type="term" value="P:ganglioside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042340">
        <property type="term" value="P:keratan sulfate proteoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000021">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000115">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000017">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026283">
        <property type="entry name" value="B-gal_1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048912">
        <property type="entry name" value="BetaGal1-like_ABD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048913">
        <property type="entry name" value="BetaGal_gal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031330">
        <property type="entry name" value="Gly_Hdrlase_35_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001944">
        <property type="entry name" value="Glycoside_Hdrlase_35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23421">
        <property type="entry name" value="BETA-GALACTOSIDASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21317">
        <property type="entry name" value="BetaGal_ABD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21467">
        <property type="entry name" value="BetaGal_gal-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01301">
        <property type="entry name" value="Glyco_hydro_35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006336">
        <property type="entry name" value="B-gal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00742">
        <property type="entry name" value="GLHYDRLASE35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012112944" description="Beta-galactosidase" evidence="18">
        <location>
            <begin position="24"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase 35 catalytic" evidence="19">
        <location>
            <begin position="35"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-galactosidase 1-like first all-beta" evidence="20">
        <location>
            <begin position="398"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-galactosidase galactose-binding" evidence="21">
        <location>
            <begin position="544"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="16">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="16">
        <location>
            <position position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031635"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00046753"/>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR006336-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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        <source>
            <dbReference type="Pfam" id="PF01301"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF21317"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF21467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017316499.1"/>
        </source>
    </evidence>
    <sequence length="655" mass="73378" checksum="0E1631617630BE8D" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QEB3</accession>
    <name>A0A2D0QEB3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Intelectin isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC108260693</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017316629.1">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070492">
        <property type="term" value="F:oligosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Intelectin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16146">
        <property type="entry name" value="INTELECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16146:SF46">
        <property type="entry name" value="INTELECTIN-1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012248879" evidence="5">
        <location>
            <begin position="20"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="6">
        <location>
            <begin position="22"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017316629.1"/>
        </source>
    </evidence>
    <sequence length="303" mass="33440" checksum="200A0A038A5B029C" modified="2017-12-20" version="1">MANWLVLLVACVSINLSLSSKEPEYFSPRSCKELMERYEIEEDGLYYLTSPTGVKYQTYCDMTTAGGGWTLVASVHENNLYGKCTTGDRWSSQQGGSPNVPDGDGSWSNTVTFGNAEAATSDDFKNPGYYDITAENVAVWHVPNNERVGRWKLTSFLRYHTESKFLRSYGGNLYNLFKIFPVKNNAGSCPANHGPSVPVVYDAGDASFNHNLFGPHVRARTDPGFIMFRAINYEKAAMAICLGVKPKDCNTEHSCIGGSGYFPEGSPRQCGDFTGLDWSGYGTHVGWSASKQLIEAAVLIFYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QED2</accession>
    <name>A0A2D0QED2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TBC1 domain family member 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Akt substrate of 160 kDa</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">tbc1d1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
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            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="14 15">Blood</tissue>
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            <dbReference type="ARBA" id="ARBA00081861"/>
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            <dbReference type="PROSITE" id="PS50086"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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        <source>
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        <source>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">SDA103</strain>
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    <dbReference type="InterPro" id="IPR036647">
        <property type="entry name" value="GTF2I-like_rpt_sf"/>
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        <property type="entry name" value="GTF2I-like repeat"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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        <source>
            <dbReference type="RefSeq" id="XP_017316105.1"/>
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    <sequence length="978" mass="108168" checksum="3C251F8306960441" modified="2017-12-20" version="1"> </sequence>
</entry>
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    <accession>A0A2D0QFX3</accession>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
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    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
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    </reference>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
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        <property type="nucleotide sequence ID" value="XM_017461137.3"/>
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    <dbReference type="KEGG" id="ipu:108260690"/>
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    <dbReference type="OrthoDB" id="6252479at2759"/>
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        <property type="component" value="Chromosome 29"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:anatomical structure morphogenesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Cadherin_repeat"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Protocadherin"/>
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        <property type="entry name" value="FAT ATYPICAL CADHERIN-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="PROTOCADHERIN FAT 4"/>
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        <property type="entry name" value="Cadherin_2"/>
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        <property type="entry name" value="Protocadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
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    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="6" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="1"/>
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            <begin position="30"/>
            <end position="1321"/>
        </location>
    </feature>
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            <begin position="891"/>
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        <location>
            <begin position="27"/>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
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            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="416"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="530"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="634"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="764"/>
            <end position="860"/>
        </location>
    </feature>
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            <begin position="188"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
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            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
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        </location>
    </feature>
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    </feature>
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        <location>
            <begin position="1282"/>
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        </location>
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            <begin position="672"/>
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        <location>
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            <end position="1199"/>
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    </feature>
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        <location>
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        <location>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="SAM" id="Phobius"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017316626.2"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QPW4</accession>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
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                <person name="Duke M.V."/>
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                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
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    <reference evidence="13" key="2">
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                <consortium name="RefSeq"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
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        <property type="component" value="Chromosome 3"/>
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    <dbReference type="GO" id="GO:0005737">
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        <property type="term" value="F:methyl-CpG binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="CXXC-TYPE ZINC FINGER PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZINC FINGER-CONTAINING"/>
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    <dbReference type="Pfam" id="PF02008">
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    </dbReference>
    <dbReference type="PROSITE" id="PS51058">
        <property type="entry name" value="ZF_CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0879">Wnt signaling pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CXXC-type" evidence="11">
        <location>
            <begin position="220"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017320447.1"/>
        </source>
    </evidence>
    <sequence length="286" mass="30852" checksum="04D3698C7CBAAEAB" modified="2017-12-20" version="1">MSNINSALCIESGANADVSLLQKDALQEAGLNQLLDYNAEIERYRSFANFYKTNGAFPQSAKIARITTPIFPSARIGVSPWNCDNAVLWGRKSAPIHPHRTRSDSSQRPGKPETLQMANGNFLSTLSPEHCRPLAGDCVNKLKCGSAAEEIMNPAFSALPALGGISLPPGVIVMTALHSPAAASAVTDSAFQIANLADCQQHNNNHSNHISSPASGGNAAKKKRKRCGVCAPCRRLINCGVCSSCRNRKTGHQICKFRKCEELKKKPGSSLERTPVSSGEAFRWFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QRH1</accession>
    <name>A0A2D0QRH1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Chromodomain Y-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC108264236</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017321058.1">
        <property type="nucleotide sequence ID" value="XM_017465569.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QRH1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009062"/>
    <dbReference type="GeneID" id="108264236"/>
    <dbReference type="KEGG" id="ipu:108264236"/>
    <dbReference type="OMA" id="QASTPKF"/>
    <dbReference type="OrthoDB" id="6357915at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18634">
        <property type="entry name" value="CD_CDY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000012">
        <property type="entry name" value="Chromodomain Y-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051053">
        <property type="entry name" value="ECH/Chromodomain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43684">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43684:SF2">
        <property type="entry name" value="CHROMODOMAIN Y-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="4">
        <location>
            <begin position="7"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="64"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017321058.1"/>
        </source>
    </evidence>
    <sequence length="490" mass="54779" checksum="F01E5BB118CFB495" modified="2017-12-20" version="1">MASGELYEVERIVDKRKNKKGKWEYLIRWKGYGSKEDTWEPEHHLLHCEEFIDQFNNLRLHDRRSKPAKASTHAKVRDASRYQGETRKKKRTSGGGAPGPGVPHRPRKMSGGKASGDRLSKPVTYKRVNAGLQFVPPIRRPTNGLQNGETDAMHYRTAARTQSIPSSRVERMMGEMEMGGAKAPLRDELGSPLPNGDAHLHSSAKRKLAEDRGYVFDKRLRYNVRQSETNYRFRDIVVRKEEGFTHVLLSSQTSDNNALTPEIMKEVCRALGAAAVDDSKLLLLSAVGSIFCSGLDASYLIGRLSTDRRKESSRIAEAVRDFVKAFIHFKKPIVVAINGPALGLGASILPLCDIVWASERAWFQTPCAALRLTPSGCSSYTFPQILGVALANEMLFCGRKLTAQEACSRGLVSQVFWPSTFSQEVLLRVKEMATCSAMVLEESKCMVRSILKSVLEEVNEKECQMLKQLWCSTKGLEALFAYLQSKGYNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QWD8</accession>
    <name>A0A2D0QWD8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Taste receptor type 1 member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC108265045</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 3 family. TAS1R subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017322619.1">
        <property type="nucleotide sequence ID" value="XM_017467130.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QWD8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000031681"/>
    <dbReference type="GeneID" id="108265045"/>
    <dbReference type="KEGG" id="ipu:108265045"/>
    <dbReference type="OMA" id="FINYKTP"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000016">
        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000004">
        <property type="entry name" value="Taste receptor type 1 member 3-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF441">
        <property type="entry name" value="TASTE RECEPTOR TYPE 1 MEMBER 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00980">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012587434" evidence="13">
        <location>
            <begin position="26"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="552"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="588"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="621"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="665"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="714"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="745"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="773"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="14">
        <location>
            <begin position="550"/>
            <end position="815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017322619.1"/>
        </source>
    </evidence>
    <sequence length="825" mass="93340" checksum="DE490A504137994B" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QWW2</accession>
    <name>A0A2D0QWW2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">choline-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="11">2.7.7.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">pcyt1ab</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the key rate-limiting step in the CDP-choline pathway for phosphatidylcholine biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>phosphocholine + CTP + H(+) = CDP-choline + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18997"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:295975"/>
            <dbReference type="EC" id="2.7.7.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:18998"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.15" evidence="11"/>
    <dbReference type="RefSeq" id="XP_017321990.1">
        <property type="nucleotide sequence ID" value="XM_017466501.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QWW2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020306"/>
    <dbReference type="GeneID" id="108264716"/>
    <dbReference type="KEGG" id="ipu:108264716"/>
    <dbReference type="CTD" id="553770"/>
    <dbReference type="OMA" id="PWECTID"/>
    <dbReference type="OrthoDB" id="17102at2759"/>
    <dbReference type="UniPathway" id="UPA00753">
        <property type="reaction ID" value="UER00739"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004105">
        <property type="term" value="F:choline-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000016">
        <property type="entry name" value="Putative choline-phosphate cytidylyltransferase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041723">
        <property type="entry name" value="CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045049">
        <property type="entry name" value="Pcy1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739:SF19">
        <property type="entry name" value="CHOLINE-PHOSPHATE CYTIDYLYLTRANSFERASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739">
        <property type="entry name" value="CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 16" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="14">
        <location>
            <begin position="75"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="309"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="310"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="348"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017321990.1"/>
        </source>
    </evidence>
    <sequence length="359" mass="41275" checksum="5E74D98DA7963376" modified="2017-12-20" version="1">MEGHSSGLTRKRRREGPNGDTDKEERGGKIPRRTSILTEPAPFSDQLETMSTPYTRVSLEEARCGIPVGRAVRVYADGIFDMFHSGHARALMQAKCLFPNTHLIVGVCSDALTHKLKGFTVMNEDERYDAVSHCRYVDEVVRDAPWTLTPEFLTKHRIDFVAHDDIPYVSDESDDVYKHIKEAGMFAPTQRTEGISTSDIITRIVRDYDLYVRRNLQRGYTAKELNVSFINEKKYHLQEHVDKVKQKVRNVEEKSKEFVQKVEEKSIDLIQKWEEKSREFIGNFLQMFGPDGALKHMLKEGKGRMLQAISPRQSPNSSPSREERSPSPTFRLPFFTKTPPSCSPPHYIDTHRISEDDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QX99</accession>
    <name>A0A2D0QX99_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13">Golgi membrane protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">golm1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12 13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GOLM family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017323033.1">
        <property type="nucleotide sequence ID" value="XM_017467544.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047011402.1">
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    </dbReference>
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    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="17"/>
            <end position="35"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="213"/>
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            <begin position="40"/>
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            <begin position="229"/>
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            <begin position="363"/>
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            <dbReference type="RefSeq" id="XP_047011402.1"/>
        </source>
    </evidence>
    <sequence length="373" mass="41192" checksum="5089E35D3E400E61" modified="2017-12-20" version="1">MIGALVNVRRGARSPPLLTVTLVACILLFGFNYWVSSSRNVELQTKLLELEEAVRRASAERAREQVGRTAAEEQVRRQTEQLALLEGAHQRRQQSAQSLWKQEKESLLLNISSTAKTVQDMKNQMKSLREDLSKVQKELQSCQNNMNTLNKKLTYDMTQCNTQILAQKEECNEKVAAAKLEVQKKYEKQNSAAAAVQPGVDSTQKAVVSKPVSEALAGNDSAENAGKPLKREPAPGAKPKTDDHLETNEIPQDQAVLTANDSDKLELPSIPLLPKQNSTLNSLTNAMEGVMDIKGGDSKDADALLDAAAVTNVKEEEDAIEYDNEGEIEKRLSKLKDDKAAAQEFDEELADYNGDDENEPEFEADKQAELAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R555</accession>
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            <fullName evidence="3">A-kinase anchor protein 11 isoform X1</fullName>
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        <name evidence="3" type="primary">akap11</name>
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        <name type="scientific">Ictalurus punctatus</name>
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            <taxon>Actinopterygii</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="3">Blood</tissue>
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    </dbReference>
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        <property type="entry name" value="SPHK1-interactor_AKAP_110"/>
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        <property type="entry name" value="A KINASE ANCHOR PROTEIN"/>
        <property type="match status" value="1"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <sequence length="1842" mass="202668" checksum="FCE26E79FB1F4630" modified="2023-06-28" version="2"> </sequence>
</entry>
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    <accession>A0A2D0R6Z5</accession>
    <name>A0A2D0R6Z5_ICTPU</name>
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        <submittedName>
            <fullName evidence="4">NXPE family member 3 isoform X1</fullName>
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        <name evidence="4" type="primary">LOC108266688</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NXPE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017325826.1">
        <property type="nucleotide sequence ID" value="XM_017470337.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R6Z5"/>
    <dbReference type="GeneID" id="108266688"/>
    <dbReference type="KEGG" id="ipu:108266688"/>
    <dbReference type="OrthoDB" id="5950832at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
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        <property type="entry name" value="NXPE4_C"/>
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    <dbReference type="InterPro" id="IPR026845">
        <property type="entry name" value="NXPH/NXPE"/>
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    <dbReference type="PANTHER" id="PTHR16165">
        <property type="entry name" value="NXPE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16165:SF9">
        <property type="entry name" value="NXPE FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06312">
        <property type="entry name" value="Neurexophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24536">
        <property type="entry name" value="NXPE4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NXPE C-terminal" evidence="2">
        <location>
            <begin position="329"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017325826.1"/>
        </source>
    </evidence>
    <sequence length="557" mass="63131" checksum="906CEF19DDB9E382" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RF71</accession>
    <name>A0A2D0RF71_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein LOC108268625</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC108268625</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017329173.1">
        <property type="nucleotide sequence ID" value="XM_017473684.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RF71"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013080"/>
    <dbReference type="GeneID" id="108268625"/>
    <dbReference type="KEGG" id="ipu:108268625"/>
    <dbReference type="OMA" id="DIATCKG"/>
    <dbReference type="OrthoDB" id="8906012at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="50"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017329173.1"/>
        </source>
    </evidence>
    <sequence length="176" mass="19605" checksum="483590D1715BADA0" modified="2017-12-20" version="1">MRYLSVGLGVAVMAMLGLVGFVLSRRNVVLQLNKTSYFESVKHKVSSDMLKEVKTNIAEANIRLERTKKQIADLTTALKTAQEAAVVKKAEMNKCNDEMNEIKTTIGALEAEKNKMEAEFQQKKASLKEQVDNLKIGAEKRSKVCDYIVKTSVDGMKLCGIVPVLQAEVKPETKQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RFG1</accession>
    <name>A0A2D0RFG1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Multifunctional methyltransferase subunit TRM112-like protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA methyltransferase 112 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">trmt112</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRM112 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017328866.1">
        <property type="nucleotide sequence ID" value="XM_017473377.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RFG1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029395"/>
    <dbReference type="GeneID" id="108268418"/>
    <dbReference type="KEGG" id="ipu:108268418"/>
    <dbReference type="CTD" id="51504"/>
    <dbReference type="OMA" id="NMLTSKC"/>
    <dbReference type="OrthoDB" id="2187549at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070476">
        <property type="term" value="P:rRNA (guanine-N7)-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21089">
        <property type="entry name" value="Trm112-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000015">
        <property type="entry name" value="Multifunctional methyltransferase subunit TRM112-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039127">
        <property type="entry name" value="Trm112"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005651">
        <property type="entry name" value="Trm112-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12773:SF0">
        <property type="entry name" value="MULTIFUNCTIONAL METHYLTRANSFERASE SUBUNIT TRM112-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12773">
        <property type="entry name" value="UPF0315 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03966">
        <property type="entry name" value="Trm112p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158997">
        <property type="entry name" value="Trm112p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017328866.1"/>
        </source>
    </evidence>
    <sequence length="123" mass="13907" checksum="D4CC52743D1EC05C" modified="2017-12-20" version="1">MKLLTHNMLTSHVKGVTKGYPLLIKATEVKVNEIEFNPQFVSRMIPKLEWSALIQAAEGLGYLQELPSTPVSNYENDEDFLRKVHRVLLEVEVIEGALQCPESGREFPISRGVPNMLLNEDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RGD2</accession>
    <name>A0A2D0RGD2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC108268464</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Secreted</location>
            <location evidence="1 11">Extracellular space</location>
            <location evidence="1 11">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017328955.1">
        <property type="nucleotide sequence ID" value="XM_017473466.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RGD2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027285"/>
    <dbReference type="GeneID" id="108268464"/>
    <dbReference type="KEGG" id="ipu:108268464"/>
    <dbReference type="OrthoDB" id="5945655at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009880">
        <property type="term" value="P:embryonic pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060897">
        <property type="term" value="P:neural plate regionalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000003">
        <property type="entry name" value="Protein Wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013301">
        <property type="entry name" value="Wnt8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF92">
        <property type="entry name" value="PROTEIN WNT-8A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01892">
        <property type="entry name" value="WNT8PROTEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11" id="KW-0879">Wnt signaling pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017328955.1"/>
        </source>
    </evidence>
    <sequence length="413" mass="45937" checksum="39F7EE3C535014BA" modified="2017-12-20" version="1">MSKLEKACHSKSATGISTVVIRACRQLLRSLYGLLHQYLSLGSLKEGIPADSDSLASMGFCQLLASLVLSVCCHVLSTTAWSVNNFLMTGPKAYLMYASSVQAGAQRGIEECKYQFAWDRWNCPESELQLSTHKGLRRATKETAFVHAISAAGVMYALTKNCSMGDFDNCGCDESKTGKNGGRGWIWGGCSDNAEFGESISKQFVDALENGHDSRAAVNRHNTEVGRLAIKATMKRSCKCHGVSGSCSVQTCWLQLSDFRDVGNYLKIKYDQAQKLELDQRRMRAGNSAENRGAIADTFSSIARTELIHLEDSPDYCVKNLSLGLHGTEGRECVQSGNKLSQFEKRSCRRLCHECGLKVEERRIEIVSSCNCKFHWCCTVKCEKCTQFVTKHNCAKRNNQSHNSSRRRQRVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RHD9</accession>
    <name>A0A2D0RHD9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Neuregulin 2b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">nrg2b</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the neuregulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017329561.1">
        <property type="nucleotide sequence ID" value="XM_017474072.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RHD9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003375"/>
    <dbReference type="GeneID" id="108268830"/>
    <dbReference type="KEGG" id="ipu:108268830"/>
    <dbReference type="CTD" id="100270726"/>
    <dbReference type="OrthoDB" id="6127080at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055013">
        <property type="term" value="P:cardiac muscle cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003007">
        <property type="term" value="P:heart morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000107">
        <property type="entry name" value="Myosin, light chain kinase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000116">
        <property type="entry name" value="pro-neuregulin-2, membrane-bound isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040180">
        <property type="entry name" value="Neuregulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002154">
        <property type="entry name" value="Neuregulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057909">
        <property type="entry name" value="NRG2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11100">
        <property type="entry name" value="HEREGULIN-NEUREGULIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11100:SF20">
        <property type="entry name" value="PRO-NEUREGULIN-2, MEMBRANE-BOUND ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02158">
        <property type="entry name" value="Neuregulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25518">
        <property type="entry name" value="NRG2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 14" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0339">Growth factor</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="7 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012700257" evidence="17">
        <location>
            <begin position="20"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="309"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="19">
        <location>
            <begin position="141"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="242"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="394"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="533"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="395"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="421"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="435"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="594"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="633"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="654"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="677"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="254"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="273"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017329561.1"/>
        </source>
    </evidence>
    <sequence length="708" mass="78845" checksum="8538E2A7FC3C2628" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RQY2</accession>
    <name>A0A2D0RQY2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Hyaluronan and proteoglycan link protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">hapln4</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017332917.1">
        <property type="nucleotide sequence ID" value="XM_017477428.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RQY2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000793"/>
    <dbReference type="GeneID" id="108270599"/>
    <dbReference type="KEGG" id="ipu:108270599"/>
    <dbReference type="CTD" id="404037"/>
    <dbReference type="OMA" id="QACLQQD"/>
    <dbReference type="OrthoDB" id="5359219at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072534">
        <property type="term" value="C:perineuronal net"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005540">
        <property type="term" value="F:hyaluronic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010001">
        <property type="term" value="P:glial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002052">
        <property type="term" value="P:positive regulation of neuroblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05877">
        <property type="entry name" value="Ig_LP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03519">
        <property type="entry name" value="Link_domain_HAPLN_module_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000011">
        <property type="entry name" value="Aggrecan core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000536">
        <property type="entry name" value="Hyaluronan and proteoglycan link protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000002">
        <property type="entry name" value="Hyaluronan proteoglycan link protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050691">
        <property type="entry name" value="Hyaluronan_bind_Proteoglycan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000538">
        <property type="entry name" value="Link_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22804">
        <property type="entry name" value="AGGRECAN/VERSICAN PROTEOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22804:SF11">
        <property type="entry name" value="HYALURONAN AND PROTEOGLYCAN LINK PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00193">
        <property type="entry name" value="Xlink"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01265">
        <property type="entry name" value="LINKMODULE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00445">
        <property type="entry name" value="LINK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01241">
        <property type="entry name" value="LINK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50963">
        <property type="entry name" value="LINK_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013016973" evidence="11">
        <location>
            <begin position="27"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="22"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Link" evidence="13">
        <location>
            <begin position="165"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Link" evidence="13">
        <location>
            <begin position="265"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017332917.1"/>
        </source>
    </evidence>
    <sequence length="382" mass="43071" checksum="D20D0E591CA039C8" modified="2017-12-20" version="1">MTVPAVKSWCLALLSCSMLLSPTVTAYSAESDKGRRKVVHVLEDDTGAVIVQTAPGKVVTHRGGTITLPCRFHHEPESVDPDRIRIKWTKVTDDFQFEDVFVALGWQQRTFGLYRGRVELEQAGPGDASIIIQNITLQDYGRYECEVTNDMEDDTGFVNLDLEGVVFPYYPRLGRYKLNYHEAEDACKQQDAILASHAQLHKAWRDGLDWCNAGWLEDGSVQYPISHPRDECGRKDTPAGVRNYGYRHKEDERYDAFCFTSKLNGQVYFLKRFKKVNYAEAVKACQRDGAALAKVGQLYAAWKFQLLDRCDAGWVDDGSVRYPIVNPRARCGGSDPGVRTLGFPDKKFRLYGAYCFRQDSGSTKAQSTQSPVPNTTSFTRSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RRH7</accession>
    <name>A0A2D0RRH7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CDAN1-interacting nuclease 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">cdin1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7 8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017332560.1">
        <property type="nucleotide sequence ID" value="XM_017477071.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017332561.1">
        <property type="nucleotide sequence ID" value="XM_017477072.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RRH7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009770"/>
    <dbReference type="GeneID" id="108270410"/>
    <dbReference type="KEGG" id="ipu:108270410"/>
    <dbReference type="CTD" id="84529"/>
    <dbReference type="OMA" id="CYWNRFG"/>
    <dbReference type="OrthoDB" id="1272at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030218">
        <property type="term" value="P:erythrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029404">
        <property type="entry name" value="CDIN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31661:SF1">
        <property type="entry name" value="CDAN1-INTERACTING NUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31661">
        <property type="entry name" value="SIMILAR TO CDNA SEQUENCE BC052040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14811">
        <property type="entry name" value="TPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017332560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017332561.1"/>
        </source>
    </evidence>
    <sequence length="285" mass="33371" checksum="D1B325D321C1282A" modified="2017-12-20" version="1">MSKMKLCRAEYRVIAKFTEQLRPTRQCMKMLKERFPNHSQSTLLSIFSLEYQKRMKRSLVKHHSDVIEDYYQRYRSEASKHPSDPVLLELANEVDLSPSLLARLLLERFLEEHQGSISSKQVLNSMLREPSLIPDVQLARHVEQCTINDCCYGPLVDCIKHAIGHEHEEILREKLRERNLSFVDENQLRAKGYDKTPDIILEVPIAVEGHIVHWIESKASFGDEHSHRTYLNEQFWSYWNRFGPGLVIYWYGFIAELDCQRDRGILLKDCFPSDIVTLGHSPDQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RSX5</accession>
    <name>A0A2D0RSX5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Myotubularin-related protein 13 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">sbf2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017333604.1">
        <property type="nucleotide sequence ID" value="XM_017478115.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000015821"/>
    <dbReference type="GeneID" id="108270995"/>
    <dbReference type="KEGG" id="ipu:108270995"/>
    <dbReference type="CTD" id="81846"/>
    <dbReference type="OMA" id="WFPQLHK"/>
    <dbReference type="OrthoDB" id="74314at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13339">
        <property type="entry name" value="PH-GRAM_MTMR13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01235">
        <property type="entry name" value="PH_Sbf1_hMTMR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000093">
        <property type="entry name" value="SET binding factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.200:FF:000004">
        <property type="entry name" value="SET binding factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11500:FF:000006">
        <property type="entry name" value="SET binding factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005112">
        <property type="entry name" value="dDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037823">
        <property type="entry name" value="MTMR13_PH-GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022096">
        <property type="entry name" value="SBF1/SBF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005113">
        <property type="entry name" value="uDENN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF4">
        <property type="entry name" value="MYOTUBULARIN-RELATED PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12335">
        <property type="entry name" value="SBF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03456">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00801">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00800">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="7">
        <location>
            <begin position="7"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Myotubularin phosphatase" evidence="8">
        <location>
            <begin position="1110"/>
            <end position="1636"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="1791"/>
            <end position="1895"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1060"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1081"/>
            <end position="1093"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017333604.1"/>
        </source>
    </evidence>
    <sequence length="1897" mass="212517" checksum="964EB0263D685CF3" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RVA4</accession>
    <name>A0A2D0RVA4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein C9orf152</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC108271369</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017334432.1">
        <property type="nucleotide sequence ID" value="XM_017478943.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RVA4"/>
    <dbReference type="GeneID" id="108271369"/>
    <dbReference type="KEGG" id="ipu:108271369"/>
    <dbReference type="OrthoDB" id="9935880at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032738">
        <property type="entry name" value="Tbc1d30_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36290">
        <property type="entry name" value="RIKEN CDNA D630039A03 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36290:SF1">
        <property type="entry name" value="RIKEN CDNA D630039A03 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15733">
        <property type="entry name" value="DUF4682"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TBC1" evidence="2">
        <location>
            <begin position="45"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="146"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017334432.1"/>
        </source>
    </evidence>
    <sequence length="248" mass="27843" checksum="C740E140B5B2E961" modified="2017-12-20" version="1">MASACCPHWPCPCDQLLKSAHFGEETRHEQEEHTDSISLPTNMDIALLQEQYNSIREKQKRETRVICFAKAPSTNEEISGKSLVNVVPLRQARHTLMRQASVQEPQFDVVTDPDNATWRTHLGMYRKTCLIPDTSQSQPCDHKTSTGDSTSTSTDNTMHSVEVTDSSDQLSEDCEGYANQHHLSNKLQEDCGSEGFRKFSAPAVLSRQLSCGSSRSTHTPMSPYYPFPQLKCPRKSEAARRLGLYSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RWV2</accession>
    <name>A0A2D0RWV2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase</fullName>
            <ecNumber evidence="6">2.1.1.62</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Cap-specific adenosine methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Phosphorylated CTD-interacting factor 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">pcif1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
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    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="13">Blood</tissue>
        </source>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CAPAM family.</text>
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        <property type="term" value="F:mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:RNA polymerase II C-terminal domain binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="WW"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PCIF1-like"/>
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        <property type="entry name" value="MRNA (2'-O-METHYLADENOSINE-N(6)-)-METHYLTRANSFERASE"/>
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    <dbReference type="Pfam" id="PF00397">
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00076956"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017334977.1"/>
        </source>
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Ictalurus punctatus</name>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Neopterygii</taxon>
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            <taxon>Ostariophysi</taxon>
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            <taxon>Ictalurus</taxon>
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    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="13">SDA103</strain>
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    <reference evidence="14 15 16" key="2">
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            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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    <dbReference type="RefSeq" id="XP_017335121.1">
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        <property type="nucleotide sequence ID" value="XM_017479633.3"/>
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        <property type="nucleotide sequence ID" value="XM_017479634.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017335124.1">
        <property type="nucleotide sequence ID" value="XM_017479635.3"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="FunFam" id="1.20.5.340:FF:000005">
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        <property type="entry name" value="Forkhead box P2 isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FH_FOXP1_P2"/>
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    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
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    <dbReference type="InterPro" id="IPR050998">
        <property type="entry name" value="FOXP"/>
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    <dbReference type="InterPro" id="IPR032354">
        <property type="entry name" value="FOXP-CC"/>
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    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
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    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45796">
        <property type="entry name" value="FORKHEAD BOX P, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45796:SF3">
        <property type="entry name" value="FORKHEAD BOX PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16159">
        <property type="entry name" value="FOXP-CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
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        <location>
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    <feature type="DNA-binding region" description="Fork-head" evidence="10">
        <location>
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            <end position="532"/>
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        <location>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="607"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
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    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017335120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017335121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017335122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017335123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017335124.1"/>
        </source>
    </evidence>
    <sequence length="715" mass="79720" checksum="785C04A2F67917D4" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S1K5</accession>
    <name>A0A2D0S1K5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">allantoinase</fullName>
            <ecNumber evidence="6">3.5.2.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">zgc:103559</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-allantoin + H2O = allantoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17029"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15678"/>
            <dbReference type="ChEBI" id="CHEBI:17536"/>
            <dbReference type="EC" id="3.5.2.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nitrogen metabolism; (S)-allantoin degradation; allantoate from (S)-allantoin: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-dependent hydrolases superfamily. Allantoinase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.5" evidence="6"/>
    <dbReference type="RefSeq" id="XP_017336085.1">
        <property type="nucleotide sequence ID" value="XM_017480596.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S1K5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020285"/>
    <dbReference type="GeneID" id="108272278"/>
    <dbReference type="KEGG" id="ipu:108272278"/>
    <dbReference type="OMA" id="SRLHVCH"/>
    <dbReference type="OrthoDB" id="1924787at2759"/>
    <dbReference type="UniPathway" id="UPA00395">
        <property type="reaction ID" value="UER00653"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004038">
        <property type="term" value="F:allantoinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000256">
        <property type="term" value="P:allantoin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006145">
        <property type="term" value="P:purine nucleobase catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000032">
        <property type="entry name" value="Allantoinase Dal1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017593">
        <property type="entry name" value="Allantoinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050138">
        <property type="entry name" value="DHOase/Allantoinase_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03178">
        <property type="entry name" value="allantoinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43668">
        <property type="entry name" value="ALLANTOINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43668:SF2">
        <property type="entry name" value="ALLANTOINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="10">
        <location>
            <begin position="86"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017336085.1"/>
        </source>
    </evidence>
    <sequence length="489" mass="53760" checksum="2D364F36B734ECCB" modified="2017-12-20" version="1">MIYLMVNWCTWQQLFFQTRGSYSLYNTKSTEMEAGSTVSAVRSLKVLIGDEVRPALITIKEGKIHNILPDPGAGTGGEVLDVGNNVVMPGLVDCHVHVNEPGRTTWEGYWTATRAAAAGGVTTIVDMPLNSIPPTTSLVNFREKLQAASGQCFVDTAFWGGVVPGNQLELRPMVRAGVAGFKCFLIHSGVDEFPHVSEKDLHAAMKQLQGTQSVLLFHAEQEVHLPMAEDGDPCEYSTFLRSRPDVMELEAIRTVTQFCLQYQVRCHIVHLSSAESLGLIRAAQQAGAPLTVETTHHYLTLNSENIPLCATQFKCCPPIRDKANQEQLWSALRAGEIDMVVSDHSPCTPDLKRLDSGDFIQAWGGISSLQFGLPLFWTSACKRGFAFPDVVKLLSEEPARLCRLDNQKGSLVPGHDADLVIWDPEKEFTVNEENIFHKNKLTPYLGLTLCGEVKGTIVRGKLVYFNGSLSPQPLGQSLLINQTLNLASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S253</accession>
    <name>A0A2D0S253_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">N-terminal EF-hand calcium-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">necab1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Interacts with STX1. May interact with CPNE6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017336275.1">
        <property type="nucleotide sequence ID" value="XM_017480786.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S253"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020121"/>
    <dbReference type="GeneID" id="108272393"/>
    <dbReference type="KEGG" id="ipu:108272393"/>
    <dbReference type="CTD" id="64168"/>
    <dbReference type="OrthoDB" id="289247at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042984">
        <property type="term" value="P:regulation of amyloid precursor protein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000025">
        <property type="entry name" value="N-terminal EF-hand calcium-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000642">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007138">
        <property type="entry name" value="ABM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039862">
        <property type="entry name" value="NECAB1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12178">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12178:SF11">
        <property type="entry name" value="N-TERMINAL EF-HAND CALCIUM-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03992">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51725">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="22"/>
            <end position="57"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00065017"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00070336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51725"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017336275.1"/>
        </source>
    </evidence>
    <sequence length="348" mass="40357" checksum="BF8CAA9C73207E2C" modified="2017-12-20" version="1">MDCSEEVQSVSDETGTPVELKKGMSIFLDILRRADKNDDGKLSFEEFKAYFSDGVLSGEELKELFHKIDTHNTDNVDTEELCAYFSQHLGEYENVLAALEDLNVSILKAMDKTKKDYQEATHLQQFVTRFLLKETTSQLQSLQSSLECAMETTAEQTRQERQAPMKPEVLSIQWSGRRSNRRLQRNNSLSPNNPLLSLVNSGMYEEDSQWMTQINRLQKLIDRLEKKELRLEPVEEEVSEGSTKSHILIVQRQLSVMEDELEEFRLALRQYVDCASVQTGSLHVAVQRIANESRFIVYEFWEHTSLWKNHLQTNYSKTFQRANVDFLETPEITTTMLVPASWWVLNNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S4A3</accession>
    <name>A0A2D0S4A3_ICTPU</name>
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        <submittedName>
            <fullName evidence="9 10">Carcinoembryonic antigen-related cell adhesion molecule 1</fullName>
        </submittedName>
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    <gene>
        <name evidence="9 10" type="primary">si:ch211-264f5.6</name>
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    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10">Blood</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_017482063.3"/>
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        <property type="nucleotide sequence ID" value="XM_047159106.2"/>
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    <dbReference type="STRING" id="7998.ENSIPUP00000007195"/>
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        <property type="component" value="Chromosome 12"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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        <property type="entry name" value="Ig_sub2"/>
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        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
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        <property type="entry name" value="IgSF_CEA-related"/>
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        <property type="entry name" value="CARCINOEMBRYONIC ANTIGEN-RELATED CELL ADHESION MOLECULE 5 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44337">
        <property type="entry name" value="CARCINOEMBRYONIC ANTIGEN-RELATED CELL ADHESION MOLECULE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044573431" evidence="6">
        <location>
            <begin position="22"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="503"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
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            <begin position="130"/>
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        </location>
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        <location>
            <begin position="406"/>
            <end position="485"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017337552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_047015062.1"/>
        </source>
    </evidence>
    <sequence length="536" mass="57493" checksum="9DB12959B4164DD0" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S786</accession>
    <name>A0A2D0S786_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Pyroglutamylated RF-amide peptide receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">AQ27</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">G-protein coupled receptor 103</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Orexigenic neuropeptide QRFP receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">SP9155</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">qrfprb</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for the orexigenic neuropeptide QRFP. The activity of this receptor is mediated by G proteins that modulate adenylate cyclase activity and intracellular calcium levels.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017338286.1">
        <property type="nucleotide sequence ID" value="XM_017482797.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S786"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000002420"/>
    <dbReference type="GeneID" id="108273524"/>
    <dbReference type="KEGG" id="ipu:108273524"/>
    <dbReference type="CTD" id="101886159"/>
    <dbReference type="OrthoDB" id="9979846at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004983">
        <property type="term" value="F:neuropeptide Y receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15205">
        <property type="entry name" value="7tmA_QRFPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000227">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000611">
        <property type="entry name" value="NPY_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF20">
        <property type="entry name" value="PYROGLUTAMYLATED RFAMIDE PEPTIDE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01012">
        <property type="entry name" value="NRPEPTIDEYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 17" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17 21" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 17" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 17 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="315"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="19">
        <location>
            <begin position="68"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        </source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017338286.1"/>
        </source>
    </evidence>
    <sequence length="392" mass="44994" checksum="B09D608162053F4A" modified="2017-12-20" version="1">MTTSTDMVSMKITPEVLQELLRYYNLSRQEFIHTYNIRPLVYVPELPAGAKAMFVIMYTLIFMLALVGNSLVVCVVVRKGSSQSATNIFICSLAISDLLITFFCIPFTLLQNISAQWLGGVLVCKTVPFVQTTAIVTGILTMTCIAVERYQGIVHPLKMKRQYTPQRAYRMLGLVWLISVMVGSPMLFVQQLEVKYDFLYENHHVCCQELWSSELQRKSYTTFIMVALFVLPLAAMLFLYTRISIELWIRKRVGDAFVLHTINHTQINKICRKKKRAVKMMITVVLLFTICWAPFHTVHMLFEYNNLEHKYDEVTLNMILAVVQAIGFSNSFNNPIVYAFMNESFKKSCVSAVSSILRRMRVHNVRVHFTKPRHATSANANTNSLELHDQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S8Q2</accession>
    <name>A0A2D0S8Q2_ICTPU</name>
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        <submittedName>
            <fullName evidence="2">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 8</fullName>
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    </protein>
    <gene>
        <name evidence="2" type="primary">ndufaf8</name>
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    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1">SDA103</strain>
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    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Blood</tissue>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017338811.1"/>
        </source>
    </evidence>
    <sequence length="70" mass="7858" checksum="95649C01F2924FA9" modified="2017-12-20" version="1">MSGRNVWTSSRERMRRFPELLARCSVEAAAYGKCVVSTTSGKQELKKDLCLKEFEALKTCFINAANQGLK</sequence>
</entry>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
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        </lineage>
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    <reference evidence="9" key="1">
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            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11 12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11 12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
            <location evidence="1 7">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017339561.2">
        <property type="nucleotide sequence ID" value="XM_017484072.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017339562.2">
        <property type="nucleotide sequence ID" value="XM_017484073.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017339564.2">
        <property type="nucleotide sequence ID" value="XM_017484075.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017339566.2">
        <property type="nucleotide sequence ID" value="XM_017484077.3"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_053684831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SAT5"/>
    <dbReference type="GeneID" id="108274153"/>
    <dbReference type="KEGG" id="ipu:108274153"/>
    <dbReference type="CTD" id="100000342"/>
    <dbReference type="OrthoDB" id="9378527at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027324">
        <property type="entry name" value="MAP2/MAP4/Tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001084">
        <property type="entry name" value="MAP_tubulin-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11501">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11501:SF14">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN TAU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00418">
        <property type="entry name" value="Tubulin-binding"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00229">
        <property type="entry name" value="TAU_MAP_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51491">
        <property type="entry name" value="TAU_MAP_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="110"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="360"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="428"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="369"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="436"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="481"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="499"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="535"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="547"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017339561.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017339562.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017339564.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017339566.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_053540806.1"/>
        </source>
    </evidence>
    <sequence length="831" mass="89002" checksum="873552A1BC0AECB9" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SJJ6</accession>
    <name>A0A2D0SJJ6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein si:dkey-261l7.2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">si:dkey-261l7.2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4 5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017342671.1">
        <property type="nucleotide sequence ID" value="XM_017487182.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017342672.1">
        <property type="nucleotide sequence ID" value="XM_017487183.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SJJ6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000014903"/>
    <dbReference type="GeneID" id="108275973"/>
    <dbReference type="KEGG" id="ipu:108275973"/>
    <dbReference type="OrthoDB" id="28868at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.390">
        <property type="entry name" value="Hemimethylated DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053189">
        <property type="entry name" value="Clp_protease_adapter_ClpF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011722">
        <property type="entry name" value="Hemimethylated_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036623">
        <property type="entry name" value="Hemimethylated_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48439">
        <property type="entry name" value="HEMIMETHYLATED DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48439:SF1">
        <property type="entry name" value="HEMIMETHYLATED DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08755">
        <property type="entry name" value="YccV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00992">
        <property type="entry name" value="YccV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141255">
        <property type="entry name" value="YccV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013510681" evidence="1">
        <location>
            <begin position="27"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Hemimethylated DNA-binding" evidence="2">
        <location>
            <begin position="115"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017342671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017342672.1"/>
        </source>
    </evidence>
    <sequence length="228" mass="26102" checksum="6E26B39DC067E62D" modified="2017-12-20" version="1">MPQVTGPVLLQLALLLCALPAQYLITQWTSGTAAQRSLATQGILDAWKSMRTSYLNVTVLVDWITSWMPKIPLLGVEEELEDMHGEIMALEILMRDNEHGYFAASEVPRSPRPAYVLHRVGEVVIDTQNHMVGVIVGWDEGLRAPPEWLKRKKYTDSEVERLKDTPHYKILFSGPDPSSLMIGYLPQNVLQLFEGYRLEIPTLDQYFSHFNGKKFVMLDWLKELYPDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SN64</accession>
    <name>A0A2D0SN64_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ceramide glucosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.80</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ugcg</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N-(9Z-octadecenoyl)-sphing-4-enine + UDP-alpha-D-xylose = beta-D-xylosyl-(1&lt;-&gt;1')-N-(9Z-octadecenoyl)-sphing-4-enine + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70247"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:77996"/>
            <dbReference type="ChEBI" id="CHEBI:189081"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:70248"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>UDP-alpha-D-xylose + an N-acylsphing-4-enine = a beta-D-xylosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70243"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:189068"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:70244"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.80" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017344148.1">
        <property type="nucleotide sequence ID" value="XM_017488659.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SN64"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030024"/>
    <dbReference type="GeneID" id="108276755"/>
    <dbReference type="KEGG" id="ipu:108276755"/>
    <dbReference type="CTD" id="7357"/>
    <dbReference type="OMA" id="IVWIIDC"/>
    <dbReference type="OrthoDB" id="1483400at2759"/>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008120">
        <property type="term" value="F:ceramide glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006679">
        <property type="term" value="P:glucosylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02520">
        <property type="entry name" value="Glucosylceramide_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000041">
        <property type="entry name" value="UDP-glucose ceramide glucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025993">
        <property type="entry name" value="Ceramide_glucosylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12726">
        <property type="entry name" value="CERAMIDE GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12726:SF0">
        <property type="entry name" value="CERAMIDE GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13506">
        <property type="entry name" value="Glyco_transf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="281"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="310"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017344148.1"/>
        </source>
    </evidence>
    <sequence length="393" mass="44406" checksum="B0F90AE478C7AE5E" modified="2017-12-20" version="1">MALLELAMQGFSIFGFILFIVLWLMHFMSIIYVRLHLNKKSGDKQPYSKLAGVSLLKPLKGVDPNLVSNLETFFELDYPKYEILLCVQDHDDPAIDVCKKLLGKYPGVDARLFIGGKKVGINPKINNLMPGYEGAKYDLVWICDSGIRVRPDTLTDMANQMNEKVGLVHGLPYVADRQGFAATLEQVYFGTSHPRSYISANVTGIKCVTGMSCLMRKDVLDQAGGLIAFAQYIAEDYFMAKAIADRGWKFSMATQVAMQNSGSYSIAQFQSRMIRWAKLRINMLPATIIEPISECFVASLIIGWAAHHMFRWDIMVFFMCHCLAWFISDYIQLRGVQGGPPSFSKLDYAVAWFIRESMTIQIFLSALWDPTISWRTGRYRLRCGGTAEEILDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SN75</accession>
    <name>A0A2D0SN75_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Neurolysin, mitochondrial isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">nln</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the peptidase M3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017344159.1">
        <property type="nucleotide sequence ID" value="XM_017488670.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SN75"/>
    <dbReference type="GeneID" id="108276763"/>
    <dbReference type="KEGG" id="ipu:108276763"/>
    <dbReference type="CTD" id="57486"/>
    <dbReference type="OrthoDB" id="534666at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06455">
        <property type="entry name" value="M3A_TOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1370.10:FF:000014">
        <property type="entry name" value="Thimet oligopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.40:FF:000001">
        <property type="entry name" value="Thimet oligopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000006">
        <property type="entry name" value="Thimet oligopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.40">
        <property type="entry name" value="Endopeptidase. Chain P, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.10">
        <property type="entry name" value="Neurolysin, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024077">
        <property type="entry name" value="Neurolysin/TOP_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024080">
        <property type="entry name" value="Neurolysin/TOP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804:SF55">
        <property type="entry name" value="NEUROLYSIN (METALLOPEPTIDASE M3 FAMILY)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M3A/M3B catalytic" evidence="10">
        <location>
            <begin position="259"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017344159.1"/>
        </source>
    </evidence>
    <sequence length="734" mass="84725" checksum="02EB43B71909BA32" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SQU6</accession>
    <name>A0A2D0SQU6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">rnf213a</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">SDA103</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="27">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="RefSeq" id="XP_017344871.2">
        <property type="nucleotide sequence ID" value="XM_017489382.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000016822"/>
    <dbReference type="GeneID" id="108277067"/>
    <dbReference type="KEGG" id="ipu:108277067"/>
    <dbReference type="CTD" id="503775"/>
    <dbReference type="OrthoDB" id="2423195at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000051">
        <property type="term" value="P:negative regulation of non-canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002040">
        <property type="term" value="P:sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16561">
        <property type="entry name" value="RING-HC_RNF213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000488">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000491">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000804">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031248">
        <property type="entry name" value="RNF213"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046439">
        <property type="entry name" value="ZF_RZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22605:SF18">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF213-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22605">
        <property type="entry name" value="RZ-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20173">
        <property type="entry name" value="ZnF_RZ-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51981">
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    <keyword evidence="17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="18" id="KW-0391">Immunity</keyword>
    <keyword evidence="9" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="19" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="16" id="KW-0862">Zinc</keyword>
    <keyword evidence="13 22" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="4088"/>
            <end position="4126"/>
        </location>
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        <location>
            <begin position="4574"/>
            <end position="4644"/>
        </location>
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        <location>
            <begin position="33"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="129"/>
            <end position="451"/>
        </location>
    </feature>
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        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="23">
        <location>
            <begin position="88"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="203"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="227"/>
            <end position="240"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="241"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="278"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="351"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="411"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="429"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_017344871.2"/>
        </source>
    </evidence>
    <sequence length="5297" mass="606126" checksum="723159BD128E0785" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SUQ4</accession>
    <name>A0A2D0SUQ4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Claudin-8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">cldn8.2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017346422.1">
        <property type="nucleotide sequence ID" value="XM_017490933.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SUQ4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003731"/>
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    <dbReference type="OMA" id="CFVAHNI"/>
    <dbReference type="OrthoDB" id="8819159at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0796">Tight junction</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="134"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="172"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017346422.1"/>
        </source>
    </evidence>
    <sequence length="297" mass="33332" checksum="2FA2DB0A6A8360F5" modified="2017-12-20" version="1">MSYTARSYGAKSYADKSYADKSYADKTYRESPYTGYDDAAKSAYEQQLLKEKKKRKEAMHCEVVALVIGFIGLIGVSTVCGLPMWKVTAFIQENIIVMETRWEGLWMNCYRQANIRMQCKVYDSLLYLPAELQAARGLMCTAVALTAIACLVSTVGMRCTRLVDSRPRVKHIVLVCGGCLFLGGCIATLIPVSWTANVIIRDFYNPLLLDAQRRELGEALYLGWVSGAVCFIAGVILLCRHAPRVEEKDDVNSVLYRAQYTPYNTAYQPGYSYQPAYNYQPAYGYQPPSVSYVPSAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SW78</accession>
    <name>A0A2D0SW78_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Nectin cell adhesion molecule 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">nectin3a</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nectin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017346947.1">
        <property type="nucleotide sequence ID" value="XM_017491458.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SW78"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026077"/>
    <dbReference type="GeneID" id="108278241"/>
    <dbReference type="KEGG" id="ipu:108278241"/>
    <dbReference type="CTD" id="793240"/>
    <dbReference type="OMA" id="FWVQDPP"/>
    <dbReference type="OrthoDB" id="9442762at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
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        <property type="entry name" value="Nectin cell adhesion molecule 3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="CD80_C2-set"/>
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        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
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    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051427">
        <property type="entry name" value="Nectin/Nectin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23277:SF12">
        <property type="entry name" value="NECTIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23277">
        <property type="entry name" value="NECTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="383"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="23"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="249"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="436"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007810"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00082570"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017346947.1"/>
        </source>
    </evidence>
    <sequence length="522" mass="57459" checksum="5C680A7CDA36FBB0" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SXF8</accession>
    <name>A0A2D0SXF8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Hepatitis A virus cellular receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">havcr1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the immunoglobulin superfamily. TIM family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347367.1">
        <property type="nucleotide sequence ID" value="XM_017491878.3"/>
    </dbReference>
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    <dbReference type="GeneID" id="108278477"/>
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    <dbReference type="OrthoDB" id="434099at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043277">
        <property type="term" value="P:apoptotic cell clearance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060097">
        <property type="term" value="P:cytoskeletal rearrangement involved in phagocytosis, engulfment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Hepatitis A virus cellular receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46608">
        <property type="entry name" value="T-CELL IMMUNOGLOBULIN AND MUCIN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46608:SF3">
        <property type="entry name" value="T-CELL IMMUNOGLOBULIN AND MUCIN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="217"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="48"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017347367.1"/>
        </source>
    </evidence>
    <sequence length="295" mass="33518" checksum="14DE46E128BCFC07" modified="2017-12-20" version="1">MQFIICETLKRKRRKVKNCVSRSKTFTMSWVYTQISSIWLLLCLTVSPCRTITVNGISGQNVTLPCKYNLSYHGKCEICWTRGEIPLTGCGKEIIATDGDKVVRQALRRYQLDGDLQKGDASLTIHNTTQKDSGRYGCRVHIPGLFNDEKIVVDLVIMKGQSPTSEVNSLLPSIPELVTKDPWYTEATQVVANSTRHYPNSLTPDINKMGKEPTDDLIPVIAVSVLLIFLVFSVAFYLIWKKKRRTRESLEIDQNQNPSAIYNNSEVSLGLNSRSMAVENIYQLDTENEYEQWSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SXL0</accession>
    <name>A0A2D0SXL0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Methyltransferase-like protein 22 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mettl22</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017347256.1">
        <property type="nucleotide sequence ID" value="XM_017491767.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SXL0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022309"/>
    <dbReference type="GeneID" id="108278413"/>
    <dbReference type="KEGG" id="ipu:108278413"/>
    <dbReference type="CTD" id="79091"/>
    <dbReference type="OMA" id="AYERIQQ"/>
    <dbReference type="OrthoDB" id="46564at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060218">
        <property type="term" value="P:hematopoietic stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038899">
        <property type="entry name" value="METTL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23108:SF0">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23108">
        <property type="entry name" value="METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="365"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="379"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017347256.1"/>
        </source>
    </evidence>
    <sequence length="390" mass="44020" checksum="D95B722AE5E803CD" modified="2017-12-20" version="1">MDKTLQHPEGREARVMDQVTFRSDTVLSDVHLLLPNAHHLMTRLNIVGQPVFLSKFRILREEEQKEACSPSEEKSYSLLDEDGDLDVARKPSETAQGRETAHPVILSQAEGAISEEEEEDDITDVIKIEHTMATPLEDVGKQIWRAAFLLSDFILSSSAAFRGATVLELGAGTGLASIVVASVAKTVYCTDVGEDLLSMCQRNVHLNQHYIAASDERGVKVRQLDWTTDDFLTDADSEFGWSEDEISDLYDNTTVIIAADVCYDNDLTDALFRTLYRISSNLRHSSTAYISIEKRLNFTLRHMDVSCETYDHFRHCLEQITELREKKMRFTVSPVSCSFPQFFQYERVNQLELWKVSVNPFQTLSSSTTSQKRGENDATSKTNPDAISKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SXU5</accession>
    <name>A0A2D0SXU5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Alkylglycerol monooxygenase</fullName>
            <ecNumber evidence="12">1.14.16.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Transmembrane protein 195</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">agmo</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Glyceryl-ether monooxygenase that cleaves the O-alkyl bond of ether lipids. Ether lipids are essential components of brain membranes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>1-O-(1,2-saturated-alkyl)-sn-glycerol + (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + O2 = a 1-(1-hydroxyalkyl)-sn-glycerol + (6R)-L-erythro-6,7-dihydrobiopterin + H2O</text>
            <dbReference type="Rhea" id="RHEA:36255"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:43120"/>
            <dbReference type="ChEBI" id="CHEBI:59560"/>
            <dbReference type="ChEBI" id="CHEBI:73418"/>
            <dbReference type="ChEBI" id="CHEBI:83957"/>
            <dbReference type="EC" id="1.14.16.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the sterol desaturase family. TMEM195 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.14.16.5" evidence="12"/>
    <dbReference type="RefSeq" id="XP_017347150.1">
        <property type="nucleotide sequence ID" value="XM_017491661.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SXU5"/>
    <dbReference type="GeneID" id="108278345"/>
    <dbReference type="KEGG" id="ipu:108278345"/>
    <dbReference type="CTD" id="392636"/>
    <dbReference type="OrthoDB" id="6354873at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050479">
        <property type="term" value="F:glyceryl-ether monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006643">
        <property type="term" value="P:membrane lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056853">
        <property type="entry name" value="AGMP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051689">
        <property type="entry name" value="Sterol_desaturase/TMEM195"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21624:SF1">
        <property type="entry name" value="ALKYLGLYCEROL MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21624">
        <property type="entry name" value="STEROL DESATURASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24858">
        <property type="entry name" value="AGMP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="367"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="17">
        <location>
            <begin position="120"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Alkylglycerol monooxygenase C-terminal" evidence="18">
        <location>
            <begin position="337"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00040992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF24858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017347150.1"/>
        </source>
    </evidence>
    <sequence length="489" mass="56172" checksum="CCE6766EDA56078C" modified="2017-12-20" version="1">MAQVEDRDDTIVSFSQGTRMLFYLMSPNETLFAEVEQVPQYVKQATPFFIGMILLEGLLGWLKTGDLLIKINDGTTSLSAGMMSRLPELFMRSLEVSSYIYVWNNFHILELPWDSAWTWWLAFLGVDFGYYWVHRCSHELNILWAAHQVHHSSEYYNLTTALRQSVTQQFASWVFYLPMALIIPPSVFAVHIQFNLLYQFWIHTEFVRDLGPLEWILNTPSHHRVHHGRNRYCIDKNYGGTLIIWDRMLGTFAPEDDKVIYGLTHPINTFDILSVEFEYYTYLLKQFISANGITHKLSVIFSGPGWTPGKPRLGDPEDLPEITGEEVPHNPHWSSALQAYVHVQFLLVLVNYNNLFATEHMLSQTTVLLITGYILLTLTILGYIMDLSPNAALWEMSRCLLMLALNRFGYISSMVPLLDLATEAMLGDSGSEGSGITGTFQALRLCLSFSLRETAGELRGSILSHQRRGVVMDRILFWPELRTRMQFMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SXW3</accession>
    <name>A0A2D0SXW3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">5-hydroxytryptamine receptor 2C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC108278586</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347523.2">
        <property type="nucleotide sequence ID" value="XM_017492034.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SXW3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000017748"/>
    <dbReference type="GeneID" id="108278586"/>
    <dbReference type="KEGG" id="ipu:108278586"/>
    <dbReference type="OrthoDB" id="420518at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030594">
        <property type="term" value="F:neurotransmitter receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007187">
        <property type="term" value="P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007208">
        <property type="term" value="P:phospholipase C-activating serotonin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007210">
        <property type="term" value="P:serotonin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247:SF32">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="42"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="272"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="311"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="22"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="206"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="206"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017347523.2"/>
        </source>
    </evidence>
    <sequence length="416" mass="46432" checksum="E72A625F10088AC6" modified="2023-06-28" version="2">MSKGKNWPALIILVIIFFTVGGNILVILAVSLEKKLHNATNFFLRSLAVADLLVGVLVMPTALINILYNYSWPLPHVLCPMWIFLDVFFCTASIMHLCAISLDRYVGIRSPIKHSRTSHSTYKAMTKIAAVWTISLVISLPVPVISLQDEQKLLVNGSCVLTEPSFILVGSSVVFFLPLIIMVVCYCLTMRVLQQHLATFSTEHRNCANTKQPSPQPSLLSDGPSSHSNSPRKGVLQHLSPPLRVRECTGPRGQGKRRMAQAIKNERRASQVLGVVFFLFLVMWCPFFVTNVLIAMCQNGCSKRLLRLLDIFVWVGYVSSGVNPLIYTLFNKNYRHAFSRYLRCSYHKQANPVLSNSACQVYSVTPTPVLCERSCTDTNANGVSGFSGSNRVYKQDEMMKEGVNNFASSMDNISRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T093</accession>
    <name>A0A2D0T093_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">E3 ubiquitin-protein ligase RBX1</fullName>
            <ecNumber evidence="13">2.3.2.32</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">RING-box protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC108279000</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine + [cullin]-L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine + N(6)-[NEDD8-protein]-yl-[cullin]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.32"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RING-box family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.32" evidence="13"/>
    <dbReference type="RefSeq" id="XP_017348348.1">
        <property type="nucleotide sequence ID" value="XM_017492859.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T093"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032281"/>
    <dbReference type="GeneID" id="108279000"/>
    <dbReference type="KEGG" id="ipu:108279000"/>
    <dbReference type="OMA" id="KWNAIAL"/>
    <dbReference type="OrthoDB" id="8962942at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031464">
        <property type="term" value="C:Cul4A-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061663">
        <property type="term" value="F:NEDD8 ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034644">
        <property type="term" value="P:cellular response to UV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16485">
        <property type="entry name" value="mRING-H2-C3H2C2D_RBX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000010">
        <property type="entry name" value="E3 ubiquitin-protein ligase RBX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051031">
        <property type="entry name" value="RING-box_E3_Ubiquitin_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024766">
        <property type="entry name" value="Znf_RING_H2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11210">
        <property type="entry name" value="RING BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12678">
        <property type="entry name" value="zf-rbx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="53"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017348348.1"/>
        </source>
    </evidence>
    <sequence length="108" mass="12296" checksum="3238A9E7823733B4" modified="2017-12-20" version="1">MAATMSVDTLSGTNSGACKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNNEWEFQKYGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T1J5</accession>
    <name>A0A2D0T1J5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Keratinocyte-associated transmembrane protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">c18h5orf15</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017348793.1">
        <property type="nucleotide sequence ID" value="XM_017493304.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T1J5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030793"/>
    <dbReference type="GeneID" id="108279232"/>
    <dbReference type="KEGG" id="ipu:108279232"/>
    <dbReference type="CTD" id="108279232"/>
    <dbReference type="OMA" id="NPEHDET"/>
    <dbReference type="OrthoDB" id="5846619at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037645">
        <property type="entry name" value="KCT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16502">
        <property type="entry name" value="KERATINOCYTE-ASSOCIATED TRANSMEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16502:SF0">
        <property type="entry name" value="KERATINOCYTE-ASSOCIATED TRANSMEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17818">
        <property type="entry name" value="KCT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013130338" evidence="3">
        <location>
            <begin position="29"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017348793.1"/>
        </source>
    </evidence>
    <sequence length="290" mass="32529" checksum="D8406F166A095FA8" modified="2017-12-20" version="1">MATLTKLWQQYSSIFLVVVLSILQLVTCASLNTQGPPMSKELEIIKNVTPASPPPSISSHYKSTSETQNLMKSNNVAIDLLTSNATASITQATPKPTSGAEMTPEDSKPIDSNQSVPKSTNLPESKPPIFTDSVKEEPSVSLDFESTTSPVEIDTNDNVGYDDEYMRLAENSQLDESKVFSQDLQDDANFIKDIAKDLPERSGKIDIHMKDTTIYATQDEDSHFFLHLVIIALLVAIVYITYHNKRKIMLLAQSRRWREGLCTRSIEYRRLDQNVDEAMPSLKMTNNYVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T758</accession>
    <name>A0A2D0T758_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Kin of IRRE-like protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Kin of irregular chiasm-like protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Nephrin-like protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">kirrel1b</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="13">Interacts with TJP1/ZO-1 and with NPHS2/podocin (via the C-terminus). Interacts with NPHS1/nephrin (via the Ig-like domains); this interaction is dependent on KIRREL1 glycosylation. Homodimer (via the Ig-like domains). Interacts when tyrosine-phosphorylated with GRB2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017350719.1">
        <property type="nucleotide sequence ID" value="XM_017495230.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T758"/>
    <dbReference type="GeneID" id="108280344"/>
    <dbReference type="CTD" id="100170816"/>
    <dbReference type="OrthoDB" id="10048737at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05898">
        <property type="entry name" value="IgI_5_KIRREL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000232">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000077">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000094">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000103">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051275">
        <property type="entry name" value="Cell_adhesion_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640:SF14">
        <property type="entry name" value="KIN OF IRRE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640">
        <property type="entry name" value="NEPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012564948" description="Kin of IRRE-like protein 1" evidence="19">
        <location>
            <begin position="21"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="499"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="22"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="121"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="224"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="309"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="393"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="619"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="739"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="627"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="17">
        <location>
            <begin position="739"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="754"/>
            <end position="768"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00064586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00074376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00076239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00082444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017350719.1"/>
        </source>
    </evidence>
    <sequence length="780" mass="85240" checksum="C9EA637E874E594B" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T7E4</accession>
    <name>A0A2D0T7E4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transgelin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">tagln</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6 7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the calponin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017350808.1">
        <property type="nucleotide sequence ID" value="XM_017495319.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017350809.1">
        <property type="nucleotide sequence ID" value="XM_017495320.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T7E4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005622"/>
    <dbReference type="GeneID" id="108280395"/>
    <dbReference type="KEGG" id="ipu:108280395"/>
    <dbReference type="CTD" id="6876"/>
    <dbReference type="GeneTree" id="ENSGT00940000155162"/>
    <dbReference type="OMA" id="VEWITVQ"/>
    <dbReference type="OrthoDB" id="21595at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000039">
        <property type="entry name" value="Transgelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050606">
        <property type="entry name" value="Calponin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000557">
        <property type="entry name" value="Calponin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003096">
        <property type="entry name" value="SM22_calponin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385">
        <property type="entry name" value="CALPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385:SF20">
        <property type="entry name" value="TRANSGELIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00402">
        <property type="entry name" value="Calponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00888">
        <property type="entry name" value="SM22CALPONIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00890">
        <property type="entry name" value="TRANSGELIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01052">
        <property type="entry name" value="CALPONIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51122">
        <property type="entry name" value="CALPONIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="4">
        <location>
            <begin position="32"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017350808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017350809.1"/>
        </source>
    </evidence>
    <sequence length="208" mass="22868" checksum="EBD8E457619DD772" modified="2017-12-20" version="1">MAAQGTPGMANKGPSYGLSRQVQDKIDKKYDPELEQRLVEWIMAQCGSGVGKPAEGKQGFQDWLKDGCVLGELINSLHDGDKPIKKIQSSSMAFKQMEQVSQFLNAAEAYGVTKTDMFQTVDLWEGKDLAAVQRTLMALGSIAVTKNDGTFRGNPDWFFKKAQENRRDFSDDQLKLGKNVIGLQMGSNKGASQAGMTGYGRPRQILNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T7W7</accession>
    <name>A0A2D0T7W7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein FAM151A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">fam151a</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the menorin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017350946.1">
        <property type="nucleotide sequence ID" value="XM_017495457.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T7W7"/>
    <dbReference type="GeneID" id="108280469"/>
    <dbReference type="KEGG" id="ipu:108280469"/>
    <dbReference type="CTD" id="338094"/>
    <dbReference type="OrthoDB" id="413402at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019356">
        <property type="entry name" value="Menorin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21184">
        <property type="entry name" value="MENORIN (DENDRITIC BRANCHING PROTEIN)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21184:SF4">
        <property type="entry name" value="PROTEIN FAM151A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10223">
        <property type="entry name" value="Menorin_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Menorin-like" evidence="9">
        <location>
            <begin position="93"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Menorin-like" evidence="9">
        <location>
            <begin position="363"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017350946.1"/>
        </source>
    </evidence>
    <sequence length="602" mass="68220" checksum="869F1F57F1D809B3" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979ENS0</accession>
    <name>A0A979ENS0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein si:dkey-12l12.1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">si:dkey-12l12.1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047006146.1">
        <property type="nucleotide sequence ID" value="XM_047150190.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979ENS0"/>
    <dbReference type="GeneID" id="124626222"/>
    <dbReference type="KEGG" id="ipu:124626222"/>
    <dbReference type="OrthoDB" id="8905635at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5037055315" evidence="1">
        <location>
            <begin position="19"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_047006146.1"/>
        </source>
    </evidence>
    <sequence length="152" mass="16873" checksum="276536CE7D800177" modified="2023-02-22" version="1">MNTVLWVFLLCMPGYLITHTLDCADGGMDCDSGLGEGVKLRFLRQTNSDHERGAHQSQANLPGAFTAKGFPNTLIQADRSRRHLNRRPVPLRSRVGSYSLLNHKTQNSPLQVVRARRHAGVAADPAMPSRMTVKQRPRSVTRKKNTKVAVCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EUN9</accession>
    <name>A0A979EUN9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leucine-rich repeat-containing protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">lrrc2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047011522.1">
        <property type="nucleotide sequence ID" value="XM_047155566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EUN9"/>
    <dbReference type="GeneID" id="108265780"/>
    <dbReference type="KEGG" id="ipu:108265780"/>
    <dbReference type="CTD" id="79442"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF16">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="388"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="399"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="432"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_047011522.1"/>
        </source>
    </evidence>
    <sequence length="482" mass="55323" checksum="2D3EDBFC88F27427" modified="2023-02-22" version="1">MKVDRTGIDIPVYDLSVIRNLWEGRVKKYKQYQKKEQERINKSALAKINQQWQYRIICRKMKTNEVTALQCYLERSAFTEMGILPTVHTSIDSEEKKFIFELSGDKWKKLPEDLQYMTYLREWHIHKTQIQKLPEYIEQFVDLCILNLPKNRLTQLPAEIGKLANLKELNMNYNKLSSIPPELGECENLEKLEMAANLDLSELPFELSNLKKLKHLDLAENKFATIPVSVLRMSSLQWLDISKNSLRDLPEDIDRMEELQTLFLHKNKLKYLPMSTANIVTLRMLVVSGDELTCLPANLTENPNLKFIKLFDNPTSLDISEDKKDNTLEQTEHDKEFMQAYIDTLKDRGTPVYVLSRRAESRRTQEGNRSFSNCRTGTTWAASCRTFRQPEGLPRQGGTDATPSSDWRTQRGNPTTQATAEEDKRARFRPGSGREYRSVKTDSVRVCSDSQSPITTVTSRPTLISLSLSLSPGGTSADETAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EX29</accession>
    <name>A0A979EX29_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15 16">Butyrophilin subfamily 1 member A1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15 16" type="primary">btr09</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15 16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. BTN/MOG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047012426.1">
        <property type="nucleotide sequence ID" value="XM_047156470.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053537909.1">
        <property type="nucleotide sequence ID" value="XM_053681934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EX29"/>
    <dbReference type="GeneID" id="108267929"/>
    <dbReference type="KEGG" id="ipu:108267929"/>
    <dbReference type="CTD" id="100170790"/>
    <dbReference type="OMA" id="FFRSAKP"/>
    <dbReference type="OrthoDB" id="10055806at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903037">
        <property type="term" value="P:regulation of leukocyte cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050863">
        <property type="term" value="P:regulation of T cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13733">
        <property type="entry name" value="SPRY_PRY_C-I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000142">
        <property type="entry name" value="V-set domain-containing T-cell activation inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053896">
        <property type="entry name" value="BTN3A2-like_Ig-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050504">
        <property type="entry name" value="IgSF_BTN/MOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100">
        <property type="entry name" value="BUTYROPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100:SF151">
        <property type="entry name" value="ICOS LIGAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22705">
        <property type="entry name" value="C2-set_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044697459" evidence="11">
        <location>
            <begin position="22"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="36"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="142"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="12">
        <location>
            <begin position="275"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_047012426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_053537909.1"/>
        </source>
    </evidence>
    <sequence length="474" mass="53756" checksum="39D9A0D36033F46F" modified="2023-02-22" version="1">MMFFCVTLLILFSFMKFQSEGLTVIGPEAPLDAVAGEDLVLPCFIKPNTSAVHMTVEWIRVEDVVSLVHLYKDHEDRNEKQAQSYRGRTSLFKEELQKGNASLKLSSLRVSDEGKYKCLIEDKSWYDDITVHVTVKVLGSHPVITMESYDHSGGINLVCESRGCKPEPEVLWMNREGDTLPAGDTKIHRDTEGFSVKDRITVSDYSESNMFYCRLLHHHHMMEAEVIINSKLFDAWKWVVGISVLAYLIAVGWIVTAVICYKKASELQKEKEYAELQRQRVEDEFIKKKKFTVDVTLDPDTAHPSLILSADGKQVTDGDTEQNLPDTPQRFDECVCVLGKQSFSSGRFYYEVQVRGKTDWTLGVVRENINRKGEITATPQNGLWTVWLRNENQYKALAGPPVPLTLREKVEKVGVFVDYEEGLVSFYDVKSSSHIYSFTGQSFTEKLYPFFSPCVNEGGKNSGPLIISPVINTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F636</accession>
    <name>A0A979F636_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Gastrin/cholecystokinin type B receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC108273896</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047015447.1">
        <property type="nucleotide sequence ID" value="XM_047159491.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979F636"/>
    <dbReference type="GeneID" id="108273896"/>
    <dbReference type="KEGG" id="ipu:108273896"/>
    <dbReference type="OrthoDB" id="10037617at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14993">
        <property type="entry name" value="7tmA_CCKR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001556">
        <property type="entry name" value="Bombsn_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241">
        <property type="entry name" value="NEUROPEPTIDE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241:SF195">
        <property type="entry name" value="OREXIN RECEPTOR TYPE 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00358">
        <property type="entry name" value="BOMBESINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 7 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="224"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="329"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="9">
        <location>
            <begin position="66"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_047015447.1"/>
        </source>
    </evidence>
    <sequence length="373" mass="41789" checksum="196EF801D4ED4966" modified="2023-02-22" version="1">MNFSSIYAKFGHLLPTNFSFLRQDHNGSVVHDSVPFAADFLFAGHEPGTIVLMVMYLVSFLTGFVGNIMALLVLSRRRNRLAGASTTRKLLINLAVCDMMVVCVCMPVNLGHQVYNAWVFGDLLCRAVPFVQAVSVSASVLSLAVISLNRYYNVHSPLRARSFFTGRKIGAMIATVWTVSSGLCAPLVFMNETKMLSLTMDGSHVITVCVETWSKARLRLGYNFLLFCALYGFPVLFNLIICFLTTWKLWSADDQFSEAAMSSSTRSAARLKSRKKIAKMVLALVVLFTLSWLPLYVVDIWIDFNMPGSLDREVPGHVEHSWVLQSRPFAQWLGLTNSALNPLCYCFVGDLYRSAKKFRKSYREKMASVFNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QNR4</accession>
    <name>A0A9F7QNR4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3 5">Apolipoprotein L1 isoform X1</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="4 6">Apolipoprotein L1 isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">LOC124627613</name>
        <name evidence="3 4" type="synonym">LOC128628834</name>
    </gene>
    <organism evidence="2 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3 4 5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3 4 5">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the apolipoprotein L family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053529749.1">
        <property type="nucleotide sequence ID" value="XM_053673774.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053529750.1">
        <property type="nucleotide sequence ID" value="XM_053673775.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053529768.1">
        <property type="nucleotide sequence ID" value="XM_053673793.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053529775.1">
        <property type="nucleotide sequence ID" value="XM_053673800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QNR4"/>
    <dbReference type="GeneID" id="124627613"/>
    <dbReference type="KEGG" id="ipu:124627613"/>
    <dbReference type="KEGG" id="ipu:128628834"/>
    <dbReference type="OrthoDB" id="6146578at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042157">
        <property type="term" value="P:lipoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008405">
        <property type="entry name" value="ApoL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14096">
        <property type="entry name" value="APOLIPOPROTEIN L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14096:SF57">
        <property type="entry name" value="APOLIPOPROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05461">
        <property type="entry name" value="ApoL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053529749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053529750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053529768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_053529775.1"/>
        </source>
    </evidence>
    <sequence length="540" mass="59885" checksum="BE6E1A830CA605FC" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QW82</accession>
    <name>A0A9F7QW82_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC128629555 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC128629555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053534125.1">
        <property type="nucleotide sequence ID" value="XM_053678150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QW82"/>
    <dbReference type="GeneID" id="128629555"/>
    <dbReference type="KEGG" id="ipu:128629555"/>
    <dbReference type="OrthoDB" id="2109241at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039471">
        <property type="entry name" value="CXorf65-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33887:SF1">
        <property type="entry name" value="GENE 867-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33887">
        <property type="entry name" value="PB1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15874">
        <property type="entry name" value="Il2rg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053534125.1"/>
        </source>
    </evidence>
    <sequence length="162" mass="18287" checksum="3692D7DB5AD1858E" modified="2023-06-28" version="1">MFATVILGEKRTELLNLNCRIVNFIHCLKEKCKLDPQAQVDLLDRTGEIMHLMEMEHSLDLVSTLLKGRQSYIVLSVSRSEGSEELQYSAVHDGDLRKSHPEVEVMLRKLSNLSKKRDKRSALKKGSVSHIKTKVTSTSTKGTTSAPGTWKKETLEPEILQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QZC3</accession>
    <name>A0A9F7QZC3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Proprotein convertase subtilisin/kexin type 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">pcsk5a</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 11 12">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053533274.1">
        <property type="nucleotide sequence ID" value="XM_053677299.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108260017"/>
    <dbReference type="KEGG" id="ipu:108260017"/>
    <dbReference type="CTD" id="571235"/>
    <dbReference type="OrthoDB" id="300641at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="CDD" id="cd04059">
        <property type="entry name" value="Peptidases_S8_Protein_convertases_Kexins_Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000006">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.850:FF:000001">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000002">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.220.10">
        <property type="entry name" value="Hormone Receptor, Insulin-like Growth Factor Receptor 1, Chain A, domain 2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.850">
        <property type="entry name" value="Peptidase S8, pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032778">
        <property type="entry name" value="GF_recep_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034182">
        <property type="entry name" value="Kexin/furin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002884">
        <property type="entry name" value="P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032815">
        <property type="entry name" value="S8_pro-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038466">
        <property type="entry name" value="S8_pro-domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF30">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14843">
        <property type="entry name" value="GF_recep_IV"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01483">
        <property type="entry name" value="P_proprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16470">
        <property type="entry name" value="S8_pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51829">
        <property type="entry name" value="P_HOMO_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 11 12" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 11 12" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4 15" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039908992" evidence="15">
        <location>
            <begin position="34"/>
            <end position="1876"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1742"/>
            <end position="1764"/>
        </location>
    </feature>
    <feature type="domain" description="P/Homo B" evidence="16">
        <location>
            <begin position="457"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="598"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1788"/>
            <end position="1816"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1828"/>
            <end position="1848"/>
        </location>
    </feature>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
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                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
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                <person name="Chen A."/>
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                <person name="Yuan Z."/>
                <person name="Yang Y."/>
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                <person name="Peatman E."/>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
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                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
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                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
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                <person name="Xie Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11">SDA103</strain>
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        <scope>IDENTIFICATION</scope>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
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                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
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                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
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    <reference evidence="3" key="2">
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
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        <property type="nucleotide sequence ID" value="XM_053682650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R4F9"/>
    <dbReference type="GeneID" id="108269564"/>
    <dbReference type="KEGG" id="ipu:108269564"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053538625.1"/>
        </source>
    </evidence>
    <sequence length="242" mass="27680" checksum="5F50E00178C2A8A6" modified="2023-06-28" version="1">MKFKNKEEEAGFGRTIWQPWPPVWRKQVGILGCESYESPFSQSQLTSCPHGWVVYYKNWREEPRNPGTDSNRHVQHNTYTTMGSELGLATWMPSSITVGMSDANRDAHLSFSHGKEWDSCLPRWAGRFRVEDESTTSDLVKVSRERLLQVDTLPITNQVHRSGKLKRMSDTPTGARLYSTRSFSNPVRVLSRARSAHLERVSSRSRSEQVQSEKKKERKKGECELEKTTSSFSLGAADLLQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RC23</accession>
    <name>A0A9F7RC23_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Syntaxin-6 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">stx6</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053539391.1">
        <property type="nucleotide sequence ID" value="XM_053683416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RC23"/>
    <dbReference type="GeneID" id="108270692"/>
    <dbReference type="CTD" id="10228"/>
    <dbReference type="OrthoDB" id="546861at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031982">
        <property type="term" value="C:vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21447">
        <property type="entry name" value="SNARE_NTD_STX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15851">
        <property type="entry name" value="SNARE_Syntaxin6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000006">
        <property type="entry name" value="Syntaxin 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.90:FF:000002">
        <property type="entry name" value="syntaxin-6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015260">
        <property type="entry name" value="Syntaxin-6/10/61_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09177">
        <property type="entry name" value="STX6_10_61_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="164"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="104"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_053539391.1"/>
        </source>
    </evidence>
    <sequence length="265" mass="30602" checksum="5388FD5DB2D5B269" modified="2023-06-28" version="1">MSMEDPFFVVKGEVQKAVNTAQGLYQRWTELLQDPGSATKEEIDWTTNELRNSLRSIEWDLEDLDETISIVEANPRKFNLDSVELNKRKAFITSTRQTVKEMKDQMASPMAMSQSDKKSRKTLMGDGGSRGPIWQPGGDKYTRLDRELQSANSQLIEEQQTQQQFIAEQQDEQLELVSGTIGVLKNMSERIGQELDEQAVMLDDFGHEIDNTQSRLDNVMKKLAKVSHMTSEKCIQFIWEELHHAEPANTTKDFMRKKWKVLDWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RDA0</accession>
    <name>A0A9F7RDA0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Extracellular calcium-sensing receptor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC108264189</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053534313.1">
        <property type="nucleotide sequence ID" value="XM_053678338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RDA0"/>
    <dbReference type="GeneID" id="108264189"/>
    <dbReference type="KEGG" id="ipu:108264189"/>
    <dbReference type="OMA" id="TIQRECT"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06364">
        <property type="entry name" value="PBP1_CaSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000016">
        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF528">
        <property type="entry name" value="C-FAMILY ODORANT RECEPTOR OLFCD2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
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            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1916"/>
            <end position="1928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1929"/>
            <end position="1944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1945"/>
            <end position="1985"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053533333.1"/>
        </source>
    </evidence>
    <sequence length="2585" mass="254921" checksum="6A9184DA72EE887D" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RFJ6</accession>
    <name>A0A9F7RFJ6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Protocadherin gamma-A11-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC128634952</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053541900.1">
        <property type="nucleotide sequence ID" value="XM_053685925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RFJ6"/>
    <dbReference type="GeneID" id="128634952"/>
    <dbReference type="KEGG" id="ipu:128634952"/>
    <dbReference type="OrthoDB" id="6252479at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF290">
        <property type="entry name" value="PROTOCADHERIN 2 ALPHA A 15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039892925" evidence="14">
        <location>
            <begin position="32"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="696"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="56"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="136"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="245"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="353"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="459"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="583"/>
            <end position="682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_053541900.1"/>
        </source>
    </evidence>
    <sequence length="821" mass="91376" checksum="F3962D62ACDFCF6B" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RGX2</accession>
    <name>A0A9F7RGX2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="1">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC108262629</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="1"/>
    <dbReference type="RefSeq" id="XP_053540405.1">
        <property type="nucleotide sequence ID" value="XM_053684430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RGX2"/>
    <dbReference type="GeneID" id="108262629"/>
    <dbReference type="KEGG" id="ipu:108262629"/>
    <dbReference type="OrthoDB" id="26387at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF28">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="243"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="251"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="277"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053540405.1"/>
        </source>
    </evidence>
    <sequence length="381" mass="42080" checksum="DD2BED1F86D5B1C6" modified="2023-06-28" version="1">MSREDPRPFLAISCPSSCDSALVCVGPRRWRARGGERRQMEMCILCHEAQEILPDGAVMVLAAFVQRSPVMSKNRKRPPHNPESCDPLFMHPDLSFGTHTGSCGHIMHSHCWQRSLNINDANREEKYNRTKDQQSEAADRQDGYLGRQDATNDYAMLRQQEEESRYKGEFVFSTETQPGTDRVTSELEPELHEVGSPAELQQRVSGEALAPGFGVRVPVKSQVPKVPAKPRVPEVQIKPRVPEFTFRPTDPNDQMPLTPSQAPLTPQSADTHRQAPLTPQSANTHRQAPPMPQSADTVHIVTSEPQPPRSAEVVAQTPRSAEVVAQPACSMATSVPPSASRRPTLLALAARGTSLGLPVQLGTSRRFHALPRKYLSLPVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RQL2</accession>
    <name>A0A9F7RQL2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Plexin-C1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC108261274</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053541618.1">
        <property type="nucleotide sequence ID" value="XM_053685643.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RQL2"/>
    <dbReference type="GeneID" id="108261274"/>
    <dbReference type="KEGG" id="ipu:108261274"/>
    <dbReference type="OrthoDB" id="384877at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002116">
        <property type="term" value="C:semaphorin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017154">
        <property type="term" value="F:semaphorin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007162">
        <property type="term" value="P:negative regulation of cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050772">
        <property type="term" value="P:positive regulation of axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00102">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031148">
        <property type="entry name" value="Plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625">
        <property type="entry name" value="PLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625:SF4">
        <property type="entry name" value="PLEXIN-C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039931218" evidence="6">
        <location>
            <begin position="18"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="862"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="domain" description="PSI" evidence="7">
        <location>
            <begin position="402"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_053541618.1"/>
        </source>
    </evidence>
    <sequence length="895" mass="100103" checksum="0636613C10C47AB2" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RQW0</accession>
    <name>A0A9F7RQW0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">A-type potassium channel modulatory protein DPP6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Dipeptidyl aminopeptidase-like protein 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Dipeptidyl peptidase 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Dipeptidyl peptidase VI</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC108269116</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="14">Homodimer (in vitro). Interacts with KCND2. Identified in a complex with KCND2 and KCNIP2. Forms an octameric complex composed of four DPP6 subunits bound to the KCND2 tetramer. Interacts with KCND3; this interaction modulates the channel gating kinetics namely channel activation and inactivation kinetics and rate of recovery from inactivation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S9B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053540383.1">
        <property type="nucleotide sequence ID" value="XM_053684408.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RQW0"/>
    <dbReference type="GeneID" id="108269116"/>
    <dbReference type="KEGG" id="ipu:108269116"/>
    <dbReference type="OrthoDB" id="16520at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF20">
        <property type="entry name" value="DIPEPTIDYL AMINOPEPTIDASE-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="17">
        <location>
            <begin position="145"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="16">
        <location>
            <begin position="592"/>
            <end position="794"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
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        </source>
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        </source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044990"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00044999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_053540383.1"/>
        </source>
    </evidence>
    <sequence length="812" mass="92576" checksum="E786AEAABD0B1845" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RT35</accession>
    <name>A0A9F7RT35_ICTPU</name>
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        <submittedName>
            <fullName evidence="13">Mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">bub1</name>
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    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
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    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
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        <subcellularLocation>
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            <location evidence="1">Centromere</location>
            <location evidence="1">Kinetochore</location>
        </subcellularLocation>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Bub1/Mad3"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR013212">
        <property type="entry name" value="Mad3/Bub1_I"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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    </dbReference>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14030">
        <property type="entry name" value="MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1"/>
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    </dbReference>
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        <property type="entry name" value="MITOTIC CHECKPOINT SERINE_THREONINE-PROTEIN KINASE BUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mad3_BUB1_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mad3_BUB1_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BUB1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
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    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BUB1 N-terminal" evidence="11">
        <location>
            <begin position="10"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="1071"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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    </feature>
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        <location>
            <begin position="288"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="686"/>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <position position="1100"/>
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        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_053542018.1"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RTY0</accession>
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        <submittedName>
            <fullName evidence="4">M-phase phosphoprotein 9</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="primary">LOC108276930</name>
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    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
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            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053543087.1">
        <property type="nucleotide sequence ID" value="XM_053687112.1"/>
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    <dbReference type="Proteomes" id="UP000221080">
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    </dbReference>
    <dbReference type="GO" id="GO:0005814">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
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    </dbReference>
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        <property type="entry name" value="M-PHASE PHOSPHOPROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14926:SF1">
        <property type="entry name" value="M-PHASE PHOSPHOPROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="404"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="756"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="841"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="925"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="563"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="213"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="242"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="273"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="286"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="305"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="416"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="440"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="475"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="775"/>
            <end position="790"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053543087.1"/>
        </source>
    </evidence>
    <sequence length="947" mass="105801" checksum="8853424AE1DA22F3" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TGT1</accession>
    <name>A0A9F7TGT1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein 585B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC108258753</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053532321.1">
        <property type="nucleotide sequence ID" value="XM_053676346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TGT1"/>
    <dbReference type="GeneID" id="108258753"/>
    <dbReference type="KEGG" id="ipu:108258753"/>
    <dbReference type="OrthoDB" id="8920688at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001772">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001289">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001954">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381:SF458">
        <property type="entry name" value="ZINC FINGER PROTEIN 585B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="439"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="467"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="495"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="523"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="551"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="166"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="385"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="385"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="400"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_053532321.1"/>
        </source>
    </evidence>
    <sequence length="578" mass="67404" checksum="60184D0543A653EF" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TGW8</accession>
    <name>A0A9F7TGW8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Claudin-10 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC108272052</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053536819.1">
        <property type="nucleotide sequence ID" value="XM_053680844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TGW8"/>
    <dbReference type="GeneID" id="108272052"/>
    <dbReference type="KEGG" id="ipu:108272052"/>
    <dbReference type="OrthoDB" id="8904666at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0796">Tight junction</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039913064" evidence="12">
        <location>
            <begin position="24"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
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            <dbReference type="Proteomes" id="UP000221080"/>
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</entry>
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    <accession>A0A9F7TKB9</accession>
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        <submittedName>
            <fullName evidence="7 8">TOG array regulator of axonemal microtubules protein 1 isoform X1</fullName>
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    <gene>
        <name evidence="7 8" type="primary">togaram1</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
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                <person name="Liu H."/>
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                <person name="Xie Y."/>
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                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="7 8">Blood</tissue>
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        <subcellularLocation>
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</entry>
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    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Liu S."/>
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                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
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                <person name="Zhang Y."/>
                <person name="Zhou T."/>
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                <person name="Gao S."/>
                <person name="Li N."/>
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                <person name="Jiang Y."/>
                <person name="Zimin A."/>
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                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
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                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
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                <person name="Xie Y."/>
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                <person name="Flanagan M.E."/>
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                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
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    <reference evidence="15" key="2">
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        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
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        <subcellularLocation>
            <location evidence="1">Secreted</location>
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    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="match status" value="4"/>
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    </dbReference>
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    </dbReference>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
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        </location>
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            <end position="4069"/>
        </location>
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            <begin position="34"/>
            <end position="204"/>
        </location>
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            <begin position="394"/>
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        <location>
            <begin position="3816"/>
            <end position="3884"/>
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        <location>
            <begin position="3957"/>
            <end position="4048"/>
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            <begin position="1269"/>
            <end position="1326"/>
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    <sequence length="4069" mass="430333" checksum="794EB350DE565F16" modified="2023-06-28" version="1"> </sequence>
</entry>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">SDA103</strain>
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    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="4">Blood</tissue>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_053542384.1"/>
        </source>
    </evidence>
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</entry>
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            <fullName evidence="1">peptide chain release factor N(5)-glutamine methyltransferase</fullName>
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        <name evidence="9" type="primary">hemk1</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Ostariophysi</taxon>
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        </lineage>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-methionine = N(5)-methyl-L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42896"/>
            <dbReference type="Rhea" id="RHEA-COMP:10271"/>
            <dbReference type="Rhea" id="RHEA-COMP:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
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            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61891"/>
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        </reaction>
    </comment>
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    </dbReference>
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    <dbReference type="GeneID" id="108272000"/>
    <dbReference type="CTD" id="51409"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:nucleic acid binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102559">
        <property type="term" value="F:peptide chain release factor N(5)-glutamine methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004556">
        <property type="entry name" value="HemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050320">
        <property type="entry name" value="N5-glutamine_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040758">
        <property type="entry name" value="PrmC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019874">
        <property type="entry name" value="RF_methyltr_PrmC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00536">
        <property type="entry name" value="hemK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03534">
        <property type="entry name" value="RF_mod_PrmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18895">
        <property type="entry name" value="HEMK METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18895:SF74">
        <property type="entry name" value="MTRF1L RELEASE FACTOR GLUTAMINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17827">
        <property type="entry name" value="PrmC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Release factor glutamine methyltransferase N-terminal" evidence="7">
        <location>
            <begin position="2"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltransferase small" evidence="6">
        <location>
            <begin position="94"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_053539747.1"/>
        </source>
    </evidence>
    <sequence length="284" mass="32367" checksum="E5151C65F4A2E408" modified="2023-06-28" version="1">MQCFQQHGISEPLLSSQYIIAHVLGKKTLQSLERRMLQERLSDKERETVWALCSKRLTRMPVQYVIEEWDFRDLTLKMRPPVFIPRPETEELVSLLLKDLRSQLGVRALRCLEVGCGSGAISLSLLHSVPQLKAVALDQSQEAVCLTKENADRLGLLDRLEIYHLDILKEADLTLRSCGPVDVIVSNPPYLFSQDMESLQAEIHRFEDHSALDGGLDGMSVIRQILTLAPRLLTDEGRMYLEVDPRHPPHIKGLVEESMLGLQYLGSHCDFTNRLRFCILQKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>E3TF63</accession>
    <name>E3TF63_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="1">PPIase</shortName>
            <ecNumber evidence="1">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PPIL6</name>
        <name evidence="5" type="synonym">ppil6</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="1"/>
    <dbReference type="EMBL" id="GU588997">
        <property type="protein sequence ID" value="ADO28949.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187660.1">
        <property type="nucleotide sequence ID" value="NM_001200731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TF63"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035646"/>
    <dbReference type="GeneID" id="100528461"/>
    <dbReference type="KEGG" id="ipu:100528461"/>
    <dbReference type="CTD" id="285755"/>
    <dbReference type="OrthoDB" id="408413at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000048">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="2">
        <location>
            <begin position="129"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADO28949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="NP_001187660.1"/>
        </source>
    </evidence>
    <sequence length="296" mass="33542" checksum="1B5AC1E1831DA328" modified="2011-01-11" version="1">MACEMQLEIVGLLKTHHFHAAKSIAEGLKQKFPTSFADPIIRPLLESDWHVYITNKRKELRGVMWQYPGLKYNVACFVSGSLLGDEKQLRGWAEKHWTFTFYRPQALYMALAQECYTQHLHSTGHVFVYMDVMIGDEAVGRMLFELFSDLCPKTCENFKALCTGEAHTSRSNLTLCYKDSLLNRVVPNGWVQGGDITGQSRGNGGESIYGPTFEDENFSVSHNKRGILGMANRGPHTNSSQLYITLQPALWMDKKYVAFGQVVEGTEVLRRLEEVPTYNERPKQDCKIVTCGLYGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-03-01" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q8UUG3</accession>
    <name>Q8UUG3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11 13">Novel immune-type receptor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">NITR2</name>
        <name evidence="13" type="synonym">nitr2</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="13832" last="13837">
            <title>Extraordinary variation in a diversified family of immune-type receptor genes.</title>
            <authorList>
                <person name="Hawke N.A."/>
                <person name="Yoder J.A."/>
                <person name="Haire R.N."/>
                <person name="Mueller M.G."/>
                <person name="Litman R.T."/>
                <person name="Miracle A.L."/>
                <person name="Stuge T."/>
                <person name="Shen L."/>
                <person name="Miller N."/>
                <person name="Litman G.W."/>
            </authorList>
            <dbReference type="PubMed" id="11698645"/>
            <dbReference type="DOI" id="10.1073/pnas.231418598"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AF397455">
        <property type="protein sequence ID" value="AAL35543.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF397467">
        <property type="protein sequence ID" value="AAL35555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187023.1">
        <property type="nucleotide sequence ID" value="NM_001200094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8UUG3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026250"/>
    <dbReference type="GeneID" id="100304508"/>
    <dbReference type="KEGG" id="ipu:100304508"/>
    <dbReference type="CTD" id="100304508"/>
    <dbReference type="GeneTree" id="ENSGT01030000234530"/>
    <dbReference type="OMA" id="SCVYHLQ"/>
    <dbReference type="OrthoDB" id="6370831at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF127">
        <property type="entry name" value="NITR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9 13" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5011323984" evidence="9 13">
        <location>
            <begin position="19"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="261"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="12"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="152"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAL35555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="NP_001187023.1"/>
        </source>
    </evidence>
    <sequence length="308" mass="34260" checksum="A1AD31921F57A572" modified="2002-03-01" version="1">MLTAGFILLLCPLHLVKTQTDFRSNDSLVFAKEGDSVNISCIYESDMAMHFSWYKYNLGQKPKLISNFYKYDKKATFHHEFKNNARFSMVNEKSKTNLEIKGLQLSDSATYFCGSAHSNIVEFGEGTELVVQASQHSLSVLQQPVHELAHPGGSVTLHCTVITDRCAGEHSVYWFRHNSGESHPGVIYTHGDSNGRCKKNPKAGSLTRMCVYSLPKTNLSTSDVGTYHCAVAACGQILFGNGTKLDMKITGLVCFENLQTLVLFSIVRSVVLLCCVILIMIYFCCSKKAKCSVRRTTKGKKNLAQLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5U9E4</accession>
    <name>W5U9E4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Phosphodiesterase</fullName>
            <ecNumber evidence="15">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">PDE6A</name>
        <name evidence="20" type="synonym">pde6a</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="15">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="15"/>
    <dbReference type="EMBL" id="JT409312">
        <property type="protein sequence ID" value="AHH38626.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017329787.1">
        <property type="nucleotide sequence ID" value="XM_017474298.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U9E4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000002155"/>
    <dbReference type="GeneID" id="108268931"/>
    <dbReference type="KEGG" id="ipu:108268931"/>
    <dbReference type="CTD" id="5145"/>
    <dbReference type="OrthoDB" id="546632at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000005">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.40:FF:000001">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.40:FF:000010">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55781">
        <property type="entry name" value="GAF domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0140">cGMP</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11" id="KW-0844">Vision</keyword>
    <feature type="domain" description="PDEase" evidence="17">
        <location>
            <begin position="484"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="800"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="12">
        <location>
            <position position="560"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="560"/>
            <end position="564"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="564"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="600"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="601"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="601"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="601"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="721"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="721"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="774"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AHH38626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017329787.1"/>
        </source>
    </evidence>
    <sequence length="858" mass="99136" checksum="84EDEB4633F6062F" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>W5U9H9</accession>
    <accession>A0A2D0SBR5</accession>
    <name>W5U9H9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3 5">SITS-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SP15</name>
        <name evidence="5" type="synonym">si:ch211-236l14.4</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2023-07" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT409293">
        <property type="protein sequence ID" value="AHH38618.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017340174.2">
        <property type="nucleotide sequence ID" value="XM_017484685.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U9H9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007798"/>
    <dbReference type="GeneID" id="108274486"/>
    <dbReference type="KEGG" id="ipu:108274486"/>
    <dbReference type="OrthoDB" id="10070917at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050985">
        <property type="entry name" value="Alpha-glycosidase_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43053">
        <property type="entry name" value="GLYCOSIDASE FAMILY 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43053:SF6">
        <property type="entry name" value="SITS-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="2">
        <location>
            <begin position="606"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AHH38618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017340174.2"/>
        </source>
    </evidence>
    <sequence length="693" mass="78231" checksum="C373AE3017ED05E7" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>W5U9T9</accession>
    <accession>A0A2D0QK50</accession>
    <name>W5U9T9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">KH domain-containing, RNA-binding, signal transduction-associated protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 14" type="primary">khdrbs2</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KHDRBS family.</text>
    </comment>
    <dbReference type="EMBL" id="JT408358">
        <property type="protein sequence ID" value="AHH38319.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017318712.2">
        <property type="nucleotide sequence ID" value="XM_017463223.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U9T9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022825"/>
    <dbReference type="GeneID" id="108262867"/>
    <dbReference type="KEGG" id="ipu:108262867"/>
    <dbReference type="CTD" id="202559"/>
    <dbReference type="OMA" id="YGHGVNE"/>
    <dbReference type="OrthoDB" id="6777263at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22469">
        <property type="entry name" value="KH-I_KHDRBS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000030">
        <property type="entry name" value="KH domain-containing, RNA-binding, signal transduction-associated protein 3 isoformX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032571">
        <property type="entry name" value="Qua1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032335">
        <property type="entry name" value="Sam68-YY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF34">
        <property type="entry name" value="KH DOMAIN-CONTAINING, RNA-BINDING, SIGNAL TRANSDUCTION-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16274">
        <property type="entry name" value="Qua1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16568">
        <property type="entry name" value="Sam68-YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="K Homology" evidence="11">
        <location>
            <begin position="58"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="178"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="321"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="340"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AHH38319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017318712.2"/>
        </source>
    </evidence>
    <sequence length="349" mass="39146" checksum="FA14C5BC7439BBA3" modified="2014-04-16" version="1">MDQDKYLPELLAEKESLDPSFVHAMRLLAEEIEKYEGDELRKDGEVKKYLDVISNKNIKLSERVLIPVQQYPKFNFVGKLLGPRGNSMKRLQEETGAKMSILGKGSMRDKDKEEELRKSGEAKYAHLNNDLHVLIEVFAPPGEAYSRMSHALEEIKKFLVPDYNDEIRQEQLRELSYLNGSDDPSRGRSVRGRGIRLTSTAATRGRGGAVPPPPPGRGAVVPRGTVGTRASLPTTMLTRGVSAPRARGTAGTPGYRAPLLQATHKTYDDYGYDDGYDGEYDEQGYEAYDDGYSNQSKSGSEYYEYGHGTNDDTYNNYAEEEWTQPRPSLKAPAARVARGGYRDHPYGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5U9U0</accession>
    <name>W5U9U0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Palmitoyltransferase</fullName>
            <ecNumber evidence="14">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Zdhhc5</name>
        <name evidence="19" type="synonym">zdhhc5a</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:36684"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="14"/>
    <dbReference type="EMBL" id="JT409609">
        <property type="protein sequence ID" value="AHH38746.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017318589.1">
        <property type="nucleotide sequence ID" value="XM_017463100.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U9U0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021128"/>
    <dbReference type="GeneID" id="108262777"/>
    <dbReference type="KEGG" id="ipu:108262777"/>
    <dbReference type="CTD" id="571795"/>
    <dbReference type="GeneTree" id="ENSGT00940000156001"/>
    <dbReference type="OMA" id="PHGVVVQ"/>
    <dbReference type="OrthoDB" id="4096362at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12349">
        <property type="entry name" value="ANKYRIN REPEAT AND LEM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12349:SF3">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="11 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="31"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="165"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="202"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="16">
        <location>
            <begin position="119"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="317"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="455"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="673"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="393"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="411"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="458"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="475"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="498"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="545"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="573"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="587"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="710"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="745"/>
            <end position="757"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AHH38746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017318589.1"/>
        </source>
    </evidence>
    <sequence length="764" mass="82998" checksum="00EAFAECF297FEFB" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5UBA8</accession>
    <name>W5UBA8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zinc finger protein 330</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Nucleolar autoantigen 36</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Znf330</name>
        <name evidence="13 14" type="synonym">znf330</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13 14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOA36 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT411047">
        <property type="protein sequence ID" value="AHH39291.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017319086.1">
        <property type="nucleotide sequence ID" value="XM_017463597.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047009888.1">
        <property type="nucleotide sequence ID" value="XM_047153932.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UBA8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008273"/>
    <dbReference type="GeneID" id="108263116"/>
    <dbReference type="KEGG" id="ipu:108263116"/>
    <dbReference type="CTD" id="27309"/>
    <dbReference type="GeneTree" id="ENSGT00390000017043"/>
    <dbReference type="OrthoDB" id="10258894at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010531">
        <property type="entry name" value="NOA36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13214">
        <property type="entry name" value="ZINC FINGER PROTEIN 330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13214:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN 330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06524">
        <property type="entry name" value="NOA36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="255"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="264"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AHH39291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017319086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_047009888.1"/>
        </source>
    </evidence>
    <sequence length="316" mass="35322" checksum="841282864D55177B" modified="2014-04-16" version="1">MPKKKTGARKKAENRKEREKQNRANREHVDVAKQACNSSMDCDKCLRRQKNRAFCYFCGLVQKLPMCAQCGKTKCMKSSDCVIKHPGVHSTGMGMVGAICDFCEAWVCHGRKCLSTHACSCPLADADCIECDRSVWEHGGRIFRCSFCHNFLCEDDQFEHQASCQVLEAETFKCVSCNRLGQHSCLRCKACFCDEHARSKVFKQEKGKAPPCPKCGHQTQETKDLSMSTRTHKFGRQTGADDDYYGASGYDSYWKSTSSGGAADQEDDDDDEDYEDDEEDEDDDEEEDEEEDEDEAEAVDSLGNLSLGVSSAGGDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5UDT9</accession>
    <name>W5UDT9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="1 3">Fanconi anemia group B protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">FANCB</name>
        <name evidence="3" type="synonym">fancb</name>
    </gene>
    <organism evidence="1">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="1">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT413296">
        <property type="protein sequence ID" value="AHH40191.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017326177.1">
        <property type="nucleotide sequence ID" value="XM_017470688.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UDT9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022639"/>
    <dbReference type="GeneID" id="108266873"/>
    <dbReference type="KEGG" id="ipu:108266873"/>
    <dbReference type="CTD" id="2187"/>
    <dbReference type="OrthoDB" id="1917888at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043240">
        <property type="term" value="C:Fanconi anaemia nuclear complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000042">
        <property type="term" value="P:negative regulation of double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905168">
        <property type="term" value="P:positive regulation of double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033333">
        <property type="entry name" value="FANCB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28450">
        <property type="entry name" value="FANCONI ANEMIA GROUP B PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28450:SF1">
        <property type="entry name" value="FANCONI ANEMIA GROUP B PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AHH40191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017326177.1"/>
        </source>
    </evidence>
    <sequence length="793" mass="89199" checksum="ABA42617AC1C1881" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5UDX8</accession>
    <name>W5UDX8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="23 25">Metalloreductase STEAP2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">STEAP2</name>
        <name evidence="25" type="synonym">LOC108278826</name>
    </gene>
    <organism evidence="23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">SDA103</strain>
        </source>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>2 Cu(+) + NADP(+) + H(+) = 2 Cu(2+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:71771"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
            <dbReference type="ChEBI" id="CHEBI:49552"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="18">
            <dbReference type="Rhea" id="RHEA:71773"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>2 Fe(2+) + NADP(+) + H(+) = 2 Fe(3+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:71767"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="19">
            <dbReference type="Rhea" id="RHEA:71769"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the STEAP family.</text>
    </comment>
    <dbReference type="EMBL" id="JT407192">
        <property type="protein sequence ID" value="AHH37892.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017347976.1">
        <property type="nucleotide sequence ID" value="XM_017492487.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UDX8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000017511"/>
    <dbReference type="GeneID" id="108278826"/>
    <dbReference type="KEGG" id="ipu:108278826"/>
    <dbReference type="GeneTree" id="ENSGT00390000008042"/>
    <dbReference type="OMA" id="HPYVKNQ"/>
    <dbReference type="OrthoDB" id="550646at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008823">
        <property type="term" value="F:cupric reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000051">
        <property type="entry name" value="STEAP2 metalloreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051267">
        <property type="entry name" value="STEAP_metalloreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14239">
        <property type="entry name" value="DUDULIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14239:SF6">
        <property type="entry name" value="METALLOREDUCTASE STEAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="15" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0410">Iron transport</keyword>
    <keyword evidence="17 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="307"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="393"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="434"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="22">
        <location>
            <begin position="35"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Ferric oxidoreductase" evidence="21">
        <location>
            <begin position="263"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF01794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="AHH37892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_017347976.1"/>
        </source>
    </evidence>
    <sequence length="491" mass="55557" checksum="0DD1C562B95E7EF7" modified="2014-04-16" version="1">MDSISLVGSSPASKASFLPNGLKYGPREGSSKPSVAIIGSGDFSKCLAIRLLHSGFHVVVGSRQPKRAAEFFPHVVDVTHHEDAVGKASIIFLAIHREHYSSLWDIKHLLAGKILVDVSNNRRSNQYPESNAEYLASLFPESNVVKGFNVISAWNMQSGPKDASREVYICSNSVDARHQVIELARQLSFIPVDMGTLSLAKEVENMPLRLFSDWKGPVLTAIALSIFFFAYSFIRDIIHPYVKNKQSFFYKIPLEMVNRTLPVVAITLLALVYLAGQLAAVHQLFYGTKFKRFPCWLQGWLQSRKQLGLLSFFFVCIHVLYSLCLPMRRSERYLLLNMAYQQVHANIENSWNEEEVWRVEMYVSFGIMSLGLLSLLAVTSIPSVNSTLNWREFSFIQSTLGYIALLIATFHALLFGWKRAFEEESYRFYMPPTFVVAVALPVTVIMGKAVLMMPCVAWRLKRIRRGCDGSKHHSDRIRPAAHVSPERITIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5UER4</accession>
    <name>W5UER4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">BMP and activin membrane-bound inhibitor homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">BAMBI</name>
        <name evidence="17" type="synonym">bambib</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 10">Negatively regulates TGF-beta signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the BAMBI family.</text>
    </comment>
    <dbReference type="EMBL" id="JT412967">
        <property type="protein sequence ID" value="AHH40064.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017351435.1">
        <property type="nucleotide sequence ID" value="XM_017495946.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UER4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000016375"/>
    <dbReference type="GeneID" id="108280666"/>
    <dbReference type="KEGG" id="ipu:108280666"/>
    <dbReference type="CTD" id="431736"/>
    <dbReference type="OMA" id="CHPESTH"/>
    <dbReference type="OrthoDB" id="5914644at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030512">
        <property type="term" value="P:negative regulation of transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090263">
        <property type="term" value="P:positive regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23576">
        <property type="entry name" value="TFP_LU_ECD_BAMBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.60.10:FF:000018">
        <property type="entry name" value="BMP and activin membrane-bound inhibitor homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009345">
        <property type="entry name" value="BAMBI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045806">
        <property type="entry name" value="BAMBI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045807">
        <property type="entry name" value="BAMBI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15505:SF1">
        <property type="entry name" value="BMP AND ACTIVIN MEMBRANE-BOUND INHIBITOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15505">
        <property type="entry name" value="RIIA DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06211">
        <property type="entry name" value="BAMBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19337">
        <property type="entry name" value="BAMBI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037456">
        <property type="entry name" value="BAMBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0732">Signal</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540192" description="BMP and activin membrane-bound inhibitor homolog" evidence="12">
        <location>
            <begin position="24"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="144"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="BMP and activin membrane-bound inhibitor N-terminal" evidence="13">
        <location>
            <begin position="2"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="BMP and activin membrane-bound inhibitor C-terminal" evidence="14">
        <location>
            <begin position="120"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF06211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF19337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AHH40064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017351435.1"/>
        </source>
    </evidence>
    <sequence length="256" mass="28705" checksum="A1223130A09F5F58" modified="2014-04-16" version="1">MERLVYIWLQLELWAMAVLLTRGEIRCYCDSPHCVATGYMCKSELNACFTRVLDPQSSKSPLSHGCYEPGLVGNGGSACRSEATQDALRPAALECCHEDMCNYRGPQDLTYGRGESTDHRSRPQAEGGRQVIARVQEMPSSKEVWFRAAVIAVPIAGGLVLVLLITLALRMLRGENQRLRQQRREMLSRLHYGFHGQHLSKSRGTKLDLECMMPLGGHENCCLTCDKIRQSDPSSQRLLSLVHWGRHGPRGKLELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5UI31</accession>
    <accession>A0A2D0QTD9</accession>
    <name>W5UI31_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein zwilch</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 12" type="primary">zwilch</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2023-07" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the RZZ complex.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Chromosome</location>
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            <location evidence="1 9">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ZWILCH family.</text>
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        <property type="protein sequence ID" value="AHH41804.1"/>
        <property type="molecule type" value="mRNA"/>
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    <dbReference type="RefSeq" id="XP_017321687.3">
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        <property type="component" value="Chromosome 4"/>
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        <property type="term" value="C:RZZ complex"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="Gene3D" id="1.10.287.1880">
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    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.730">
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR018630">
        <property type="entry name" value="Zwilch"/>
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    <dbReference type="PANTHER" id="PTHR15995">
        <property type="entry name" value="PROTEIN ZWILCH HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15995:SF1">
        <property type="entry name" value="PROTEIN ZWILCH HOMOLOG"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF09817">
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    </dbReference>
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    <keyword evidence="7 9" id="KW-0131">Cell cycle</keyword>
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    <keyword evidence="5 9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AHH41804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017321687.3"/>
        </source>
    </evidence>
    <sequence length="584" mass="66354" checksum="7C8C7DBE634D97CA" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
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    <name>W5UI35_ICTPU</name>
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        </submittedName>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein LOC108280525</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Harbi1</name>
        <name evidence="11" type="synonym">LOC108280525</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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            <taxon>Ostariophysi</taxon>
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            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT415746">
        <property type="protein sequence ID" value="AHH41809.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017351072.1">
        <property type="nucleotide sequence ID" value="XM_017495583.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UI35"/>
    <dbReference type="GeneID" id="108280525"/>
    <dbReference type="KEGG" id="ipu:108280525"/>
    <dbReference type="GeneTree" id="ENSGT00940000163250"/>
    <dbReference type="OMA" id="MFLMTYM"/>
    <dbReference type="OrthoDB" id="2668416at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF236">
        <property type="entry name" value="PROTEIN ALP1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="8">
        <location>
            <begin position="211"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AHH41809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017351072.1"/>
        </source>
    </evidence>
    <sequence length="436" mass="50340" checksum="393DFDFC630B5FCE" modified="2014-04-16" version="1">MANEEALKSLMFTLSYMLMKRRRDINSAETQRRREVQRRVRHRQYFLQRQRRMLMMLIAQGTRPVTVARTRAWSNTPSTDWWERVVLTEFQPSDWLERFRMSKETFFHICNKLKPWLARQDTCLRPALPLEKRVALVLWRLASNVEYRTIGELFGVGRSTVCKCVREVCHAIGATLRPLYLREPSEQELEDAARLFSTRWGFPHCVGAVSSLHVPIIAPSADADSYWNSRGWHSVITQGTVNGLGQFWDVCAGFPGGSEPASILQNSSLWTLGCEGRLLPQSLPRNFMGKQLGSVILGDAGYPLQTWLLKAYDKSHNLTAGQRAFNRRLERARAIVDEAFLRLRARWQCLLKRNDCHVDGVPAMIVACCVLHNMCEVHGDELAEEWLEAARREESPMPTHMVPVLDDDPAGEEVRELFCQYFLQQEHQQNQQEQQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5UII4</accession>
    <name>W5UII4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4 6 7">Protein-associating with the carboxyl-terminal domain of ezrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">Scyl3</name>
        <name evidence="6 7 8" type="synonym">scyl3</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6 7 8" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6 7 8">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="JT415912">
        <property type="protein sequence ID" value="AHH41862.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017311421.1">
        <property type="nucleotide sequence ID" value="XM_017455932.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017311422.1">
        <property type="nucleotide sequence ID" value="XM_017455933.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047006787.1">
        <property type="nucleotide sequence ID" value="XM_047150831.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UII4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035722"/>
    <dbReference type="GeneID" id="108257843"/>
    <dbReference type="KEGG" id="ipu:108257843"/>
    <dbReference type="CTD" id="57147"/>
    <dbReference type="GeneTree" id="ENSGT00930000151043"/>
    <dbReference type="OMA" id="HDPELDW"/>
    <dbReference type="OrthoDB" id="9942861at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF15">
        <property type="entry name" value="PROTEIN-ASSOCIATING WITH THE CARBOXYL-TERMINAL DOMAIN OF EZRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="485"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="730"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="518"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="533"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="557"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="574"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="614"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AHH41862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017311421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017311422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_047006787.1"/>
        </source>
    </evidence>
    <sequence length="750" mass="82539" checksum="5E0BF35E32479CBE" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5UKG5</accession>
    <name>W5UKG5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Alpha-2-antiplasmin</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="7">Serpin peptidase inhibitor, clade F (Alpha-2 antiplasmin, pigment epithelium derived factor), member 2b isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Serpinf2</name>
        <name evidence="7" type="synonym">serpinf2b</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="EMBL" id="JT418236">
        <property type="protein sequence ID" value="AHH42664.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017346443.1">
        <property type="nucleotide sequence ID" value="XM_017490954.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UKG5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006293"/>
    <dbReference type="MEROPS" id="I04.023"/>
    <dbReference type="GeneID" id="108277904"/>
    <dbReference type="CTD" id="563663"/>
    <dbReference type="OMA" id="DKHMVDV"/>
    <dbReference type="OrthoDB" id="9947020at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF20">
        <property type="entry name" value="ALPHA-2-ANTIPLASMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540316" evidence="3">
        <location>
            <begin position="20"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Serpin" evidence="4">
        <location>
            <begin position="106"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AHH42664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017346443.1"/>
        </source>
    </evidence>
    <sequence length="475" mass="52383" checksum="84F0615B5CD05A0D" modified="2014-04-16" version="1">MDLRLLVILFLCYCQQGWTEDVISEDENVSVIPFMSPMATSSPPIDVVSSRVSHTEEFTPDGFVTSSSTAENAESSSEEDLDELCGPLNSKELRQAIGNGIMKFGLELLENLKADAKQSNIIISPLSVSLALSQLALGARNDTEELLLQRLHVDTVPCYHKALKSLLHHVRKNALQIASRIYLADGFKPNQEFIEESLKIYDSKPAALTDLEEINEWVEKSTNGHVTDFLSSLPPNLIMMLINAVHYKGEWLRRFDPHFTSSEPFYVDEKHIVNVDMMLGPKYPLSLFNHNELDAQIARFPFKGNVSLIIVMPTIGSVDISAIAAKLNISDLYNHLPRERSMQVKLPKFKLDFTQELEEVLTNMGLGELFSSPNLGGISAGPLLVSSVQHKSSIEINEEGAEAAAATSVAISRSNPSFTVNQPFFLALIEDSTKTPLFLGIISNPNPSGTAVTVGSSLLEKGEFPSTKHFDKPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5ULJ6</accession>
    <name>W5ULJ6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">CSGALNACT2</name>
        <name evidence="17 18" type="synonym">csgalnact2</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17 18">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>3-O-(beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Xyl)-L-seryl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = 3-O-(beta-D-GalNAc-(1-&gt;4)-beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Xyl)-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23464"/>
            <dbReference type="Rhea" id="RHEA-COMP:12573"/>
            <dbReference type="Rhea" id="RHEA-COMP:12575"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="ChEBI" id="CHEBI:132093"/>
            <dbReference type="ChEBI" id="CHEBI:132105"/>
            <dbReference type="EC" id="2.4.1.174"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Golgi apparatus</location>
            <location evidence="1 13">Golgi stack membrane</location>
            <topology evidence="1 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the chondroitin N-acetylgalactosaminyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="EMBL" id="JT418925">
        <property type="protein sequence ID" value="AHH42897.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017320015.1">
        <property type="nucleotide sequence ID" value="XM_017464526.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017320016.1">
        <property type="nucleotide sequence ID" value="XM_017464527.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ULJ6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000031950"/>
    <dbReference type="GeneID" id="108263569"/>
    <dbReference type="KEGG" id="ipu:108263569"/>
    <dbReference type="CTD" id="55454"/>
    <dbReference type="GeneTree" id="ENSGT01050000244968"/>
    <dbReference type="OMA" id="GMMVFRE"/>
    <dbReference type="OrthoDB" id="431432at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047238">
        <property type="term" value="F:glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047237">
        <property type="term" value="F:glucuronylgalactosylproteoglycan 4-beta-N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050650">
        <property type="term" value="P:chondroitin sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015012">
        <property type="term" value="P:heparan sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000059">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008428">
        <property type="entry name" value="Chond_GalNAc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051227">
        <property type="entry name" value="CS_glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12369:SF20">
        <property type="entry name" value="CHONDROITIN SULFATE N-ACETYLGALACTOSAMINYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12369">
        <property type="entry name" value="CHONDROITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05679">
        <property type="entry name" value="CHGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 13 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="65"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AHH42897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017320015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017320016.1"/>
        </source>
    </evidence>
    <sequence length="540" mass="62455" checksum="B2CD58832A7EF8F9" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5UMD7</accession>
    <name>W5UMD7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Anaphase-promoting complex subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Cyclosome subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Mitotic checkpoint regulator</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Testis-specific gene 24 protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ANAPC1</name>
        <name evidence="22" type="synonym">anapc1</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">The mammalian APC/C is composed at least of 14 distinct subunits ANAPC1, ANAPC2, CDC27/APC3, ANAPC4, ANAPC5, CDC16/APC6, ANAPC7, CDC23/APC8, ANAPC10, ANAPC11, CDC26/APC12, ANAPC13, ANAPC15 and ANAPC16 that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa; APC/C interacts with FZR1 and FBXO5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the APC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT414990">
        <property type="protein sequence ID" value="AHH40832.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017320052.1">
        <property type="nucleotide sequence ID" value="XM_017464563.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000027434"/>
    <dbReference type="GeneID" id="108263589"/>
    <dbReference type="KEGG" id="ipu:108263589"/>
    <dbReference type="CTD" id="64682"/>
    <dbReference type="GeneTree" id="ENSGT00390000016757"/>
    <dbReference type="OMA" id="MQPPDSR"/>
    <dbReference type="OrthoDB" id="26401at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007091">
        <property type="term" value="P:metaphase/anaphase transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000075">
        <property type="entry name" value="Anaphase promoting complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000103">
        <property type="entry name" value="Anaphase promoting complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024990">
        <property type="entry name" value="Apc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048971">
        <property type="entry name" value="Apc1_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041221">
        <property type="entry name" value="APC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046794">
        <property type="entry name" value="Apc1_MidN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049255">
        <property type="entry name" value="Apc1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12827:SF3">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12827">
        <property type="entry name" value="MEIOTIC CHECKPOINT REGULATOR TSG24 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12859">
        <property type="entry name" value="ANAPC1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21282">
        <property type="entry name" value="APC1_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18122">
        <property type="entry name" value="APC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20518">
        <property type="entry name" value="Apc1_MidN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 1 N-terminal" evidence="16">
        <location>
            <begin position="85"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 1 N-terminal" evidence="16">
        <location>
            <begin position="552"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 1 middle" evidence="18">
        <location>
            <begin position="666"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 1 beta-sandwich" evidence="19">
        <location>
            <begin position="1620"/>
            <end position="1705"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 1 C-terminal" evidence="17">
        <location>
            <begin position="1739"/>
            <end position="1900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="315"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="323"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF12859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF18122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF20518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF21282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="AHH40832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017320052.1"/>
        </source>
    </evidence>
    <sequence length="1959" mass="217996" checksum="294B7EE8DAB0613E" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5UR75</accession>
    <name>W5UR75_ICTPU</name>
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        <submittedName>
            <fullName evidence="14 16">SH3 domain-binding protein 4-A</fullName>
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    </protein>
    <gene>
        <name evidence="14 16" type="primary">sh3bp4a</name>
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    <organism evidence="14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
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    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Clathrin-coated vesicle</location>
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        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="AHH42103.1"/>
        <property type="molecule type" value="mRNA"/>
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    <dbReference type="RefSeq" id="XP_017314504.1">
        <property type="nucleotide sequence ID" value="XM_017459015.3"/>
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    <dbReference type="AlphaFoldDB" id="W5UR75"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009269"/>
    <dbReference type="GeneID" id="108259487"/>
    <dbReference type="KEGG" id="ipu:108259487"/>
    <dbReference type="CTD" id="403082"/>
    <dbReference type="OMA" id="KHQFILA"/>
    <dbReference type="OrthoDB" id="10000126at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
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    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="SH3 domain-binding protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056183">
        <property type="entry name" value="DEATH_SH3BP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="SH3_domain"/>
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        <property type="entry name" value="SH3BP4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056182">
        <property type="entry name" value="UPA_SH3BP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000906">
        <property type="entry name" value="ZU5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15603:SF3">
        <property type="entry name" value="SH3 DOMAIN-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15603">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24094">
        <property type="entry name" value="DEATH_SH3BP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23637">
        <property type="entry name" value="SH3BP4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23640">
        <property type="entry name" value="UPA_SH3BP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00791">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51145">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
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    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0728">SH3 domain</keyword>
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        <location>
            <begin position="56"/>
            <end position="115"/>
        </location>
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    <feature type="domain" description="ZU5" evidence="13">
        <location>
            <begin position="319"/>
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        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="12">
        <location>
            <begin position="654"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AHH42103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017314504.1"/>
        </source>
    </evidence>
    <sequence length="963" mass="108011" checksum="9CC88AEE685CF3E9" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5URD5</accession>
    <name>W5URD5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein lin-7 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Lin7b</name>
        <name evidence="15" type="synonym">lin7b</name>
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    <organism evidence="13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
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        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the lin-7 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT416865">
        <property type="protein sequence ID" value="AHH42173.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017314692.1">
        <property type="nucleotide sequence ID" value="XM_017459203.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5URD5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020718"/>
    <dbReference type="GeneID" id="108259591"/>
    <dbReference type="KEGG" id="ipu:108259591"/>
    <dbReference type="CTD" id="64130"/>
    <dbReference type="GeneTree" id="ENSGT00940000153222"/>
    <dbReference type="OMA" id="TDMATMT"/>
    <dbReference type="OrthoDB" id="10056216at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097025">
        <property type="term" value="C:MPP7-DLG1-LIN7 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007269">
        <property type="term" value="P:neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06796">
        <property type="entry name" value="PDZ_Lin-7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000076">
        <property type="entry name" value="Protein lin-7 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.650">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014775">
        <property type="entry name" value="L27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036892">
        <property type="entry name" value="L27_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017365">
        <property type="entry name" value="LIN7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051109">
        <property type="entry name" value="MAM_complex_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14063">
        <property type="entry name" value="PROTEIN LIN-7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02828">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038039">
        <property type="entry name" value="Lin-7_homologue"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00569">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101288">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51022">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 10" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="L27" evidence="12">
        <location>
            <begin position="20"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="11">
        <location>
            <begin position="103"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR038039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AHH42173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017314692.1"/>
        </source>
    </evidence>
    <sequence length="217" mass="23957" checksum="BE31B9832A2D6B6F" modified="2014-04-16" version="1">MMSSYYHPGKEADMAEPLCLERDVCRVIELLDRLQRSGELPPSKLQALQRVLQSKFCAAIREVYEQLYDTLDIVGGPEVRAQATAKATVAAFAASEGHAHPRVVELPKTDEGLGFNIMGGKEQNSPIYISRVIPGGVADRQGGLKRGDQLLSVNGVSVEGEHHEKAVELLKAAQGSVKLVVRYTPKVLEEMEARFEKLRSARRRQQHTSHSSLESRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5URR7</accession>
    <name>W5URR7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ferrochelatase</fullName>
            <ecNumber evidence="14">4.98.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15 17" type="primary">fech</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the ferrous insertion into protoporphyrin IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>heme b + 2 H(+) = protoporphyrin IX + Fe(2+)</text>
            <dbReference type="Rhea" id="RHEA:22584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="EC" id="4.98.1.1"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:22586"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Porphyrin-containing compound metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the ferrochelatase family.</text>
    </comment>
    <dbReference type="EC" id="4.98.1.1" evidence="14"/>
    <dbReference type="EMBL" id="JT417261">
        <property type="protein sequence ID" value="AHH42318.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053543869.1">
        <property type="nucleotide sequence ID" value="XM_053687894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5URR7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022271"/>
    <dbReference type="GeneID" id="108279312"/>
    <dbReference type="KEGG" id="ipu:108279312"/>
    <dbReference type="CTD" id="2235"/>
    <dbReference type="GeneTree" id="ENSGT00390000016258"/>
    <dbReference type="OrthoDB" id="1323at2759"/>
    <dbReference type="UniPathway" id="UPA00252">
        <property type="reaction ID" value="UER00325"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00419">
        <property type="entry name" value="Ferrochelatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03411">
        <property type="entry name" value="Ferrochelatase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1400:FF:000003">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1400">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00323">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001015">
        <property type="entry name" value="Ferrochelatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019772">
        <property type="entry name" value="Ferrochelatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033644">
        <property type="entry name" value="Ferrochelatase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033659">
        <property type="entry name" value="Ferrochelatase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00109">
        <property type="entry name" value="hemH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108">
        <property type="entry name" value="FERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108:SF1">
        <property type="entry name" value="FERROCHELATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00762">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53800">
        <property type="entry name" value="Chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00534">
        <property type="entry name" value="FERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="9 14" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7 14" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="11 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 14" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12 14" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AHH42318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_053543869.1"/>
        </source>
    </evidence>
    <sequence length="412" mass="46619" checksum="2700D4815F6BE916" modified="2014-04-16" version="1">MQMALLGGMCRFVRLSRSSRHVGWCVGASLRRQSTARAASALATPSSPKTQDKRKPKTGILMLNMGGPERLEDVHDFLLRLFLDKDLMQLPIQHKLGPFIAKRRTPKIQEQYSKIGGGSPIRAWTTMQGEGMVKLLDEMSPKTAPHKFYIGFRYVRPLTEEAIEEMESDGVERAVAFTQYPQYSCSTTGSSLNAIYRYYSSSGLSPKMRWSVIDRWPTHPLLIECFAQHVRKELEKFPPEKRDDVVILFSAHSLPMSVVNRGDPYPQEVGATVQRVMERLAYCNPYRLVWQSKVGPMPWLGPQTDDVIKGLCKRGKKNLLLVPIAFTSDHIETLHELDIEYSQILGEECGVENIRRAESLNGNPLFFKALADLVQTHLKSDEQCSRQLTLRCPLCVNPTCGKTKAFFASQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0B5GUN7</accession>
    <name>A0A0B5GUN7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Claudin 3d</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="13">Claudin-3-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 13" type="primary">CLDN3d</name>
        <name evidence="13" type="synonym">LOC108279106</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13" key="1">
        <citation type="journal article" date="2015" name="Comp. Biochem. Physiol. Part D Genomics Proteomics" volume="13" first="60" last="69">
            <title>Claudin multigene family in channel catfish and their expression profiles in response to bacterial infection and hypoxia as revealed by meta-analysis of RNA-Seq datasets.</title>
            <authorList>
                <person name="Sun L."/>
                <person name="Liu S."/>
                <person name="Bao L."/>
                <person name="Li Y."/>
                <person name="Feng J."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="25681604"/>
            <dbReference type="DOI" id="10.1016/j.cbd.2015.01.002"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="EMBL" id="KM870801">
        <property type="protein sequence ID" value="AJF34915.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001316458.1">
        <property type="nucleotide sequence ID" value="NM_001329529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0B5GUN7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000036749"/>
    <dbReference type="GeneID" id="108279106"/>
    <dbReference type="KEGG" id="ipu:108279106"/>
    <dbReference type="GeneTree" id="ENSGT00940000162095"/>
    <dbReference type="OMA" id="RYGPPSK"/>
    <dbReference type="OrthoDB" id="8830244at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000001">
        <property type="entry name" value="Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0796">Tight junction</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AJF34915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="NP_001316458.1"/>
        </source>
    </evidence>
    <sequence length="220" mass="23565" checksum="3E61852CF587ADEC" modified="2015-04-01" version="1">MAALGLEILGISLSVLGCILSIVCCALPMWRVSAFIGNNIITAQVFWEGIWMNCVYQSTGQMQCKVYDSILALPQDLQAARALTVVAVVLGILALLVSIVGAKCTNCIEDEVAKARVMIVSGVIFITAAITQLIPVSWSANKIIREFYSPIVPEAQKREIGASLYLGWGAAAMLLVGGSILCCSCPPAEKQAKYIPASHVAYSTTKRSVAPSSYSRKDYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PHD7</accession>
    <name>A0A2D0PHD7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">C-C chemokine receptor type 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC108254854</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017305758.1">
        <property type="nucleotide sequence ID" value="XM_017450269.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PHD7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000001412"/>
    <dbReference type="GeneID" id="108254854"/>
    <dbReference type="KEGG" id="ipu:108254854"/>
    <dbReference type="OMA" id="CEYGTHA"/>
    <dbReference type="OrthoDB" id="8951197at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14984">
        <property type="entry name" value="7tmA_Chemokine_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000355">
        <property type="entry name" value="Chemokine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF627">
        <property type="entry name" value="C-C CHEMOKINE RECEPTOR TYPE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00657">
        <property type="entry name" value="CCCHEMOKINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="60"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017305758.1"/>
        </source>
    </evidence>
    <sequence length="355" mass="39850" checksum="0C20407A8E9037F0" modified="2017-12-20" version="1">MAAMLNTSTGLWEITTPSVFSTTEYDYSDVGIFQLCEYGTHASRILPVLYSLFFVVGFLGNMLVLWVILRGPQMKSMTDVSLLNLVIADLLLIFSLPSQAHYARDTWVFGRAMCTLVLSVYYIGFYSGIFFIVLMSIDRYLAIVHAVFILRIRTKIYGILASLVVWIIAIAASFPELLYLVKENGTEVVCSAYPKIQESHNDVRSAAFFKMNILGMLIPLSIVGFCYSMILQKLRTVRKSGKLAIRLVAAVVVVFFCCWIPYNIAAFLKALEMKRILPPGCELSKTIQLMLQVTEAIAYSHSCLNPFLYVFVGQKFRRNLAKLLLETPCVKVQCVNRYLTRAAAPVNDDEGSVGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PNW1</accession>
    <name>A0A2D0PNW1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein tmem176l.1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tmem176l.1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MS4A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017308278.1">
        <property type="nucleotide sequence ID" value="XM_017452789.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PNW1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009190"/>
    <dbReference type="GeneID" id="108256187"/>
    <dbReference type="KEGG" id="ipu:108256187"/>
    <dbReference type="CTD" id="100330405"/>
    <dbReference type="OMA" id="KALCYNP"/>
    <dbReference type="OrthoDB" id="8951938at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007237">
        <property type="entry name" value="CD20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030417">
        <property type="entry name" value="MS4A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MS4A -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320:SF125">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 176L.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04103">
        <property type="entry name" value="CD20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017308278.1"/>
        </source>
    </evidence>
    <sequence length="252" mass="27782" checksum="95A1A1A8F34FA9DE" modified="2017-12-20" version="1">MSLMISRAEGVTVYTVQSNPKSKWPLLCQILGTMCYSPVCSVSLRLKQQIGCALTALATIQIMVGIMNLGFGILSTAIGDLGLAPFWLGGMFIAVGIMCILAEKFPSPCLVVLTVVMNIVSSALAITAIVLYSVDLAQIRYIYGCELPKREPDDRYYWTTASPSKKAQLDELFEIKLENYRRCEQNKHLIQTICGGLDIMMIVLAVLQLCVTISWCVLNTKALCKNNADGQDMEDPELCKPLMEPETPNPEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PP85</accession>
    <name>A0A2D0PP85_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Purine-rich element binding protein Ba</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">purba</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PUR DNA-binding protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017307817.1">
        <property type="nucleotide sequence ID" value="XM_017452328.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PP85"/>
    <dbReference type="GeneID" id="108255942"/>
    <dbReference type="KEGG" id="ipu:108255942"/>
    <dbReference type="CTD" id="565233"/>
    <dbReference type="OMA" id="HTETIIN"/>
    <dbReference type="OrthoDB" id="523901at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032422">
        <property type="term" value="F:purine-rich negative regulatory element binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.700:FF:000001">
        <property type="entry name" value="Purine-rich element binding protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2450.30:FF:000001">
        <property type="entry name" value="Purine-rich element binding protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2450.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006628">
        <property type="entry name" value="PUR-bd_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12611">
        <property type="entry name" value="PUR-TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12611:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN PUR-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04845">
        <property type="entry name" value="PurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00712">
        <property type="entry name" value="PUR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="282"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="282"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="295"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017307817.1"/>
        </source>
    </evidence>
    <sequence length="305" mass="33366" checksum="C9E594EB052AAAD1" modified="2017-12-20" version="1">MADGDSGSERGGSSGGGGGSGFPHFHRDQEETQELASKRLDIQNKRFYLDVKQNSKGRFVKIAEVGAGGSKSRLTLSMSVAAEFRDYLGDFIEHYAQLGPSTPEQIAQSSGGEDGGPRRALKSEFLVRENRKYYLDLKENQRGRFLRIRQTVNRGAGFGIGGGVAPGAAMQSGQTIALPAQGLIEFRDALAKLIDDYGGDDDELAGPGCGAGGYSELPEGTSITVDSKRFFFDVGANKYGVFLRVSEVKPSYRNSITVPFKAWSKFGGAFCRYAEEMKEIQERQRDKMYERRADEESEGDDVDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PQ60</accession>
    <name>A0A2D0PQ60_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Solute carrier family 2, facilitated glucose transporter member 11b isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">slc2a11b</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017307571.1">
        <property type="nucleotide sequence ID" value="XM_017452082.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A2D0PQ60"/>
    <dbReference type="GeneID" id="108255822"/>
    <dbReference type="KEGG" id="ipu:108255822"/>
    <dbReference type="CTD" id="564970"/>
    <dbReference type="OrthoDB" id="8120565at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046323">
        <property type="term" value="P:D-glucose import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070837">
        <property type="term" value="P:dehydroascorbic acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17432">
        <property type="entry name" value="MFS_GLUT_Class2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000029">
        <property type="entry name" value="solute carrier family 2, facilitated glucose transporter member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF1">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="329"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="386"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="426"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="455"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="36"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017307571.1"/>
        </source>
    </evidence>
    <sequence length="513" mass="56286" checksum="50F961ADF0B8F88C" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PQ97</accession>
    <name>A0A2D0PQ97_ICTPU</name>
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        <submittedName>
            <fullName evidence="14">Carboxypeptidase A6 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">cpa6</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017308189.1">
        <property type="nucleotide sequence ID" value="XM_017452700.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PQ97"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019014"/>
    <dbReference type="GeneID" id="108256134"/>
    <dbReference type="KEGG" id="ipu:108256134"/>
    <dbReference type="CTD" id="57094"/>
    <dbReference type="OMA" id="HQHAREH"/>
    <dbReference type="OrthoDB" id="3626597at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Carboxypeptidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000001">
        <property type="entry name" value="Carboxypeptidase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.340">
        <property type="entry name" value="Metallocarboxypeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057247">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036990">
        <property type="entry name" value="M14A-like_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003146">
        <property type="entry name" value="M14A_act_pep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF18">
        <property type="entry name" value="CARBOXYPEPTIDASE A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02244">
        <property type="entry name" value="Propep_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00133">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 14" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M14" evidence="12">
        <location>
            <begin position="135"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="11">
        <location>
            <position position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017308189.1"/>
        </source>
    </evidence>
    <sequence length="436" mass="50437" checksum="564C29F14A968A2C" modified="2017-12-20" version="1">MASEVKVKRACAALVLGWLVFSNAASAYLYNNRYVGDQVLRIIPSSPGEIHYLKQLFQNITVDLWQPSSSNLIHEGKAVDVHVGRDRTARLKNLLRQAHVQHELLISDVQREMEKQTGYRSNRKRRSVLRYDYEVYHPLEEIQSWMFEVNRTHPHLVDLFSIGRSYEGRPLYVLQLGNRVRTSRKAVWMDCGVHAREWIGPAFCQWFVKEALNSYQHDSSMRRLLKQLNFYIMPVFNVDGYHYSWKHDRFWRKTRSKKAKYHCRGVDANRNFKVKWCEEGASTHPCDDTYCGPFPESEPEVKAVSRFLRKHKKHTRAYISIHAYAQMLLYPYSYKYGTIPNFDCVETAAQSAVSALYSAYGVRYRYGPASTTLYVSSGSSIDWAYKNGIPYAFAFELRDTGHYGFLLPEALIGPTCTETMRAVRAIASGLLKKCTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PSQ9</accession>
    <name>A0A2D0PSQ9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Septin-9 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">septin12</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017309052.1">
        <property type="nucleotide sequence ID" value="XM_017453563.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PSQ9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022680"/>
    <dbReference type="GeneID" id="108256559"/>
    <dbReference type="KEGG" id="ipu:108256559"/>
    <dbReference type="CTD" id="124404"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000143">
        <property type="entry name" value="septin-9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="10">
        <location>
            <begin position="521"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="89"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="281"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="374"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="414"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="100"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="117"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="296"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017309052.1"/>
        </source>
    </evidence>
    <sequence length="820" mass="90980" checksum="8CDD4FCD2B3DAA9E" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PT92</accession>
    <name>A0A2D0PT92_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD(+) ADP-ribosyltransferase</fullName>
            <ecNumber evidence="1">2.4.2.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC108256662</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>NAD(+) + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H(+).</text>
            <dbReference type="EC" id="2.4.2.30"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.30" evidence="1"/>
    <dbReference type="RefSeq" id="XP_017309232.1">
        <property type="nucleotide sequence ID" value="XM_017453743.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PT92"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027214"/>
    <dbReference type="GeneID" id="108256662"/>
    <dbReference type="KEGG" id="ipu:108256662"/>
    <dbReference type="OMA" id="RSPTTWM"/>
    <dbReference type="OrthoDB" id="539213at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09518">
        <property type="entry name" value="SAM_ANKS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000025">
        <property type="entry name" value="Ankyrin repeat and sterile alpha motif domain-containing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF9">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="69"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="102"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="135"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="190"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="224"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="325"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="359"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="10">
        <location>
            <begin position="802"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="501"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="591"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="637"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="704"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="610"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="637"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="709"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="738"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="755"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="782"/>
            <end position="799"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017309232.1"/>
        </source>
    </evidence>
    <sequence length="907" mass="98423" checksum="F19122D9515D61C5" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PY35</accession>
    <name>A0A2D0PY35_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9 10">Cystatin-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9 10" type="primary">LOC108257254</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8 9 10" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8 9 10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cystatin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017310346.1">
        <property type="nucleotide sequence ID" value="XM_017454857.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017310347.1">
        <property type="nucleotide sequence ID" value="XM_017454858.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017310349.1">
        <property type="nucleotide sequence ID" value="XM_017454860.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PY35"/>
    <dbReference type="GeneID" id="108257254"/>
    <dbReference type="KEGG" id="ipu:108257254"/>
    <dbReference type="OrthoDB" id="6115262at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001713">
        <property type="entry name" value="Prot_inh_stefin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11414:SF21">
        <property type="entry name" value="CYSTATIN 14A, TANDEM DUPLICATE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11414">
        <property type="entry name" value="CYSTATIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00031">
        <property type="entry name" value="Cystatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00295">
        <property type="entry name" value="STEFINA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0789">Thiol protease inhibitor</keyword>
    <feature type="domain" description="Cystatin" evidence="6">
        <location>
            <begin position="13"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017310346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017310347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017310349.1"/>
        </source>
    </evidence>
    <sequence length="99" mass="11191" checksum="68787E1F16736665" modified="2017-12-20" version="1">MSTCIAGGWTKWRTTDDDDVKNICELVKPEAEEMEKTKFTVYVPLNYRSQVVAGINYEMKVFVGNDSCLFLRVFQGPGPDPESVLQRTTVFSLPSIITH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PZJ4</accession>
    <name>A0A2D0PZJ4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Discoidin, CUB and LCCL domain-containing protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">dcbld1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017311447.1">
        <property type="nucleotide sequence ID" value="XM_017455958.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PZJ4"/>
    <dbReference type="GeneID" id="108257858"/>
    <dbReference type="KEGG" id="ipu:108257858"/>
    <dbReference type="CTD" id="285761"/>
    <dbReference type="OMA" id="HDGDYQR"/>
    <dbReference type="OrthoDB" id="441660at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000005">
        <property type="entry name" value="Procollagen C-endopeptidase enhancer 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.20">
        <property type="entry name" value="LCCL-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004043">
        <property type="entry name" value="LCCL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036609">
        <property type="entry name" value="LCCL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050633">
        <property type="entry name" value="Neuropilin_MCO_CoagFactor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806:SF1">
        <property type="entry name" value="DISCOIDIN, CUB AND LCCL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806">
        <property type="entry name" value="F5/8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03815">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00603">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69848">
        <property type="entry name" value="LCCL domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50820">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012045045" evidence="10">
        <location>
            <begin position="24"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="442"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="11">
        <location>
            <begin position="30"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="LCCL" evidence="13">
        <location>
            <begin position="150"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="12">
        <location>
            <begin position="246"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="623"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="698"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017311447.1"/>
        </source>
    </evidence>
    <sequence length="717" mass="79427" checksum="2447B778991C207E" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q2M3</accession>
    <name>A0A2D0Q2M3_ICTPU</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein si:dkey-225f5.4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">si:dkey-225f5.4</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017312537.1">
        <property type="nucleotide sequence ID" value="XM_017457048.3"/>
    </dbReference>
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    <dbReference type="STRING" id="7998.ENSIPUP00000003688"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="term" value="C:kinetochore"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="ZW10 INTERACTOR"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zwint"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="114"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017312537.1"/>
        </source>
    </evidence>
    <sequence length="378" mass="42675" checksum="06135A353C7F5374" modified="2017-12-20" version="1">MATERAAVILERTGPSLLKTCDSTEAQDSGEGKVMTAYLMDCRRKQKIMCHQHGVMDDMLKLLAGLESAEQFLNEPCPPNPDTEASTPWKALKAEYLEQVQEVEGMIGTLVECMEELLKKRQRLEALLVDLEKKKEECKEQERAALQSKQRAEKLMNLQIDDSLQRAQDALRACDRRLSELKIQVDDRLAKASNWMAFRDRLQATLEVTQANMQYKLLSASPSELCLELLPRSTQQSLQPLHLSITMTKEDHFRLQVFQGTAGLLEESVEGPVRELSAALLEVMECYISQGKMLAEIQALHSRFAIDWCPAKRLLIFLKSATTVCHLELDEGYPSSGCAKLLAVRKDGNLLNLSALQPIVMNPTLTDWLEFLSSYPDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q6Z5</accession>
    <name>A0A2D0Q6Z5_ICTPU</name>
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        <submittedName>
            <fullName evidence="15">ADP-ribosylation factor-binding protein GGA1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC108258754</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
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        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Early endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GGA protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017313125.1">
        <property type="nucleotide sequence ID" value="XM_017457636.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q6Z5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019784"/>
    <dbReference type="GeneID" id="108258754"/>
    <dbReference type="KEGG" id="ipu:108258754"/>
    <dbReference type="OMA" id="HRKADSL"/>
    <dbReference type="OrthoDB" id="447025at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034394">
        <property type="term" value="P:protein localization to cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008153">
        <property type="entry name" value="GAE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004152">
        <property type="entry name" value="GAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038425">
        <property type="entry name" value="GAT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027422">
        <property type="entry name" value="GGA1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041198">
        <property type="entry name" value="GGA_N-GAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45905:SF6">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-BINDING PROTEIN GGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45905">
        <property type="entry name" value="GOLGI-LOCALIZED, GAMMA-ADAPTIN EAR CONTAINING, ARF BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02883">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03127">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18308">
        <property type="entry name" value="GGA_N-GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00809">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50180">
        <property type="entry name" value="GAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50909">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="VHS" evidence="11">
        <location>
            <begin position="18"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="GAT" evidence="13">
        <location>
            <begin position="172"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="GAE" evidence="12">
        <location>
            <begin position="441"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="403"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017313125.1"/>
        </source>
    </evidence>
    <sequence length="570" mass="64566" checksum="29D269824D9F7118" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QAY5</accession>
    <name>A0A2D0QAY5_ICTPU</name>
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        <submittedName>
            <fullName evidence="6">Golgin subfamily A member 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">golga2</name>
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    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017315848.1">
        <property type="nucleotide sequence ID" value="XM_017460359.3"/>
    </dbReference>
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    <dbReference type="STRING" id="7998.ENSIPUP00000028035"/>
    <dbReference type="GeneID" id="108260253"/>
    <dbReference type="KEGG" id="ipu:108260253"/>
    <dbReference type="CTD" id="2801"/>
    <dbReference type="OrthoDB" id="5978643at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000137">
        <property type="term" value="C:Golgi cis cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024858">
        <property type="entry name" value="GOLGA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043937">
        <property type="entry name" value="GOLGA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043976">
        <property type="entry name" value="GOLGA_cons_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10881:SF46">
        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10881">
        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19046">
        <property type="entry name" value="GM130_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15070">
        <property type="entry name" value="GOLGA2L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Golgin subfamily A conserved" evidence="4">
        <location>
            <begin position="389"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
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            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
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            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="959"/>
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        </location>
    </feature>
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        <location>
            <begin position="174"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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            <end position="853"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="882"/>
            <end position="909"/>
        </location>
    </feature>
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        <location>
            <begin position="49"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="458"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="966"/>
            <end position="977"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017315848.1"/>
        </source>
    </evidence>
    <sequence length="1036" mass="118596" checksum="EB8446FDB9BFD3D8" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QEM8</accession>
    <name>A0A2D0QEM8_ICTPU</name>
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        <recommendedName>
            <fullName evidence="10">Lysosomal cobalamin transport escort protein LMBD1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">LMBR1 domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">lmbrd1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LIMR family. LMBRD1 subfamily.</text>
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    <dbReference type="RefSeq" id="XP_017317128.1">
        <property type="nucleotide sequence ID" value="XM_017461639.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QEM8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008736"/>
    <dbReference type="GeneID" id="108260915"/>
    <dbReference type="KEGG" id="ipu:108260915"/>
    <dbReference type="CTD" id="55788"/>
    <dbReference type="OMA" id="FWAQFVF"/>
    <dbReference type="OrthoDB" id="73273at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061462">
        <property type="term" value="P:protein localization to lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050854">
        <property type="entry name" value="LMBD1_LysCbl_Transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006876">
        <property type="entry name" value="LMBR1-like_membr_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16130:SF2">
        <property type="entry name" value="LYSOSOMAL COBALAMIN TRANSPORT ESCORT PROTEIN LMBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16130">
        <property type="entry name" value="LYSOSOMAL COBALAMIN TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04791">
        <property type="entry name" value="LMBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="9" id="KW-0170">Cobalt</keyword>
    <keyword evidence="8" id="KW-0458">Lysosome</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="193"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="370"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="412"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="482"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00039618"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00042599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017317128.1"/>
        </source>
    </evidence>
    <sequence length="534" mass="61320" checksum="A100AAE791D42F32" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QIB1</accession>
    <name>A0A2D0QIB1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="24">Neuronal acetylcholine receptor subunit alpha-6 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">chrna6</name>
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    <organism evidence="23 24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Synaptic cell membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017317255.1">
        <property type="nucleotide sequence ID" value="XM_017461766.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QIB1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000002350"/>
    <dbReference type="GeneID" id="108260984"/>
    <dbReference type="KEGG" id="ipu:108260984"/>
    <dbReference type="CTD" id="8973"/>
    <dbReference type="OrthoDB" id="5975154at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19016">
        <property type="entry name" value="LGIC_ECD_nAChR_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19064">
        <property type="entry name" value="LGIC_TM_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000008">
        <property type="entry name" value="Cholinergic receptor nicotinic alpha 6 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000051">
        <property type="entry name" value="Cholinergic receptor, nicotinic, alpha 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000001">
        <property type="entry name" value="Neuronal nicotinic acetylcholine receptor subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 24" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 20" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012429215" evidence="20">
        <location>
            <begin position="47"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="257"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="288"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="497"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="21">
        <location>
            <begin position="50"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="22">
        <location>
            <begin position="263"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="376"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="418"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="428"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_017317255.1"/>
        </source>
    </evidence>
    <sequence length="523" mass="60403" checksum="C04A1241C66687F4" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QIV5</accession>
    <name>A0A2D0QIV5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Histone H3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC108261414</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="13">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the histone H3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017317801.2">
        <property type="nucleotide sequence ID" value="XM_017462312.3"/>
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    <dbReference type="GeneID" id="108261414"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000078">
        <property type="entry name" value="Histone H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000044">
        <property type="entry name" value="Histone H3.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00322">
        <property type="entry name" value="HISTONE_H3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="11 13" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="10 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="15">
        <location>
            <begin position="45"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017317801.2"/>
        </source>
    </evidence>
    <sequence length="136" mass="15300" checksum="8093BF3E55162BF7" modified="2023-06-28" version="1">MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYXAGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVGLFEDTNLCAIHAKRVTIMPXDIQLARRIRGERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QJ08</accession>
    <name>A0A2D0QJ08_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Interferon-inducible GTPase 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC108262224</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class dynamin-like GTPase superfamily. IRG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017318302.1">
        <property type="nucleotide sequence ID" value="XM_017462813.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QJ08"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029761"/>
    <dbReference type="GeneID" id="108262224"/>
    <dbReference type="KEGG" id="ipu:108262224"/>
    <dbReference type="OMA" id="IEADCIR"/>
    <dbReference type="OrthoDB" id="422720at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000541">
        <property type="entry name" value="Immunity related GTPase M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030385">
        <property type="entry name" value="G_IRG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007743">
        <property type="entry name" value="Immunity-related_GTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051515">
        <property type="entry name" value="IRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341">
        <property type="entry name" value="INTERFERON-INDUCIBLE GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32341:SF17">
        <property type="entry name" value="IRG-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05049">
        <property type="entry name" value="IIGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51716">
        <property type="entry name" value="G_IRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IRG-type G" evidence="5">
        <location>
            <begin position="41"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017318302.1"/>
        </source>
    </evidence>
    <sequence length="397" mass="44042" checksum="A4A38EB6CBA465B9" modified="2017-12-20" version="1">MASQFGISEGEMNEMNSVLNSRMVTDVVAQVEGMLAQMKSVTLNIAVTGESGAGKSSFINAFRGVSDDEPEAAETGVTETTQQALAYSHPTASNVLLWDLPGIGTPTFQAGTYLEDVGLLKYDFFIIVTSDRFQEYHSALAKYIMQAQKKFYFIRNKVDRDLEANARRRSQRGLSDDDVLDLLRVNCEKNLRNGQVEQPQVFLLSCFHPQHYDFPALQMTLLEELEGHKRHALLLSLPNLSASLIQSKRKALDQDVWKKAVVACLSSVTRGATPNPIVPKLMETLKYYQQTFSLDAKSLHRLANLTGISFEKLQSEVTSTFGRELSAQAVEDLLSQVASGHQLLANQLENRVPILGSVIAGGISFVASYFLLKSALKDLSEDGERVIQKSFESKHRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QK64</accession>
    <name>A0A2D0QK64_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Interferon-induced protein 44-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC108261682</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SDA103</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017317985.1">
        <property type="nucleotide sequence ID" value="XM_017462496.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QK64"/>
    <dbReference type="GeneID" id="108261682"/>
    <dbReference type="KEGG" id="ipu:108261682"/>
    <dbReference type="OrthoDB" id="25620at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14241">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14241:SF1">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_017317985.1"/>
        </source>
    </evidence>
    <sequence length="87" mass="9944" checksum="B4431FE2025F2BE1" modified="2017-12-20" version="1">MTKVDETCPLVKDDLRKVYTSKKIKEKMQECSDLLGIPLSNIFPVKNYQEEVDTNNDMDVLILKALDQIVNLTNDALEDQNPSEKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QP79</accession>
    <name>A0A2D0QP79_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">KH domain-containing, RNA-binding, signal transduction-associated protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Src-associated in mitosis 68 kDa protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">khdrbs1a</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the KHDRBS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017320114.1">
        <property type="nucleotide sequence ID" value="XM_017464625.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QP79"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029238"/>
    <dbReference type="GeneID" id="108263614"/>
    <dbReference type="KEGG" id="ipu:108263614"/>
    <dbReference type="CTD" id="30082"/>
    <dbReference type="OMA" id="PYEESYA"/>
    <dbReference type="OrthoDB" id="6777263at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000036">
        <property type="entry name" value="KH RNA binding domain containing, signal transduction associated 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032571">
        <property type="entry name" value="Qua1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032335">
        <property type="entry name" value="Sam68-YY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF51">
        <property type="entry name" value="KH DOMAIN CONTAINING, RNA BINDING, SIGNAL TRANSDUCTION ASSOCIATED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16274">
        <property type="entry name" value="Qua1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16568">
        <property type="entry name" value="Sam68-YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 19" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="12" id="KW-0729">SH3-binding</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="K Homology" evidence="21">
        <location>
            <begin position="79"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="196"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="363"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="20">
        <location>
            <begin position="210"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="226"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="20">
        <location>
            <begin position="239"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="285"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="378"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00070445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00078343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017320114.1"/>
        </source>
    </evidence>
    <sequence length="387" mass="42475" checksum="D5ED1EE1E4F5D35A" modified="2017-12-20" version="1">MDANRNNTNKGKKPNNNGTNPNMESESKYLPELLAEKDSLDASFTHAMKLLTAEIERIQKGEPKKDGETYLDLFTAKNVRVKERVLIPVKQYPRFNFVGKILGPQGSTIKRLQEETGAKISVLGKGSMRDKNKEEELRKGGEPKYAHLSMELHVYIEVFAPIPECYLRMAHAMDEVKKFLVPVDNMDEMHQDPFMDVGYLNGAQDAPTRGRGGPGRGRGGPPLPMPGARGRGMLPRGGAPRGGAPRGGPGRGGAGRGAPVGRGGHPSSSSNRGGAAGRSRPPTASQRMATAPSLSHQQHQGQESYEEYSYDETYAETPYEGYENYYNQSAPQGETEYYDYGHGEAQENYESYAEDDWEGAAWGSGGGKAPAARQNKGNYREHPYGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QPV2</accession>
    <name>A0A2D0QPV2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">SPARC-related modular calcium-binding protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">smoc2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017320344.1">
        <property type="nucleotide sequence ID" value="XM_017464855.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QPV2"/>
    <dbReference type="GeneID" id="108263750"/>
    <dbReference type="KEGG" id="ipu:108263750"/>
    <dbReference type="CTD" id="64094"/>
    <dbReference type="OrthoDB" id="5986054at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050840">
        <property type="term" value="F:extracellular matrix binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00191">
        <property type="entry name" value="TY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000076">
        <property type="entry name" value="SPARC-related modular calcium binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.10:FF:000004">
        <property type="entry name" value="SPARC-related modular calcium-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.10:FF:000003">
        <property type="entry name" value="SPARC-related modular calcium-binding protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.10">
        <property type="entry name" value="Thyroglobulin type-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051950">
        <property type="entry name" value="Dev_reg/Prot_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019577">
        <property type="entry name" value="SPARC/Testican_Ca-bd-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000716">
        <property type="entry name" value="Thyroglobulin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036857">
        <property type="entry name" value="Thyroglobulin_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12352">
        <property type="entry name" value="SECRETED MODULAR CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12352:SF21">
        <property type="entry name" value="SPARC-RELATED MODULAR CALCIUM-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10591">
        <property type="entry name" value="SPARC_Ca_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16597">
        <property type="entry name" value="Thyroglob_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00086">
        <property type="entry name" value="Thyroglobulin_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00211">
        <property type="entry name" value="TY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57610">
        <property type="entry name" value="Thyroglobulin type-1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00484">
        <property type="entry name" value="THYROGLOBULIN_1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51162">
        <property type="entry name" value="THYROGLOBULIN_1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013016959" evidence="11">
        <location>
            <begin position="22"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="13">
        <location>
            <begin position="32"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Thyroglobulin type-1" evidence="12">
        <location>
            <begin position="84"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Thyroglobulin type-1" evidence="12">
        <location>
            <begin position="219"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="425"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="429"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="121"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="257"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017320344.1"/>
        </source>
    </evidence>
    <sequence length="444" mass="49699" checksum="4E9931BCC4E3AB54" modified="2017-12-20" version="1">MRDSVLLLLLLVFGLLQEARAQKLSALTFLRVDQDKECNMDCSSAQRKPLCASDGRTFASRCEFQRAKCRDPQLEVMRGSCKDTSRCAAEKKYTEQQAKKLFPQVFVPVCNPDGTYSEVQCHSFTGYCWCVTPNGRPISGTAVANKKPRCQGSKHEKVTPREPGKAVSLQIFSILNADESVVLVEETQPPADEEDMISQYPTLWTEQVRSRQNRTRTQTSSCDQEQLTAQEEARQHKNEAVFIPDCAQGGLYKPVQCHPSTGYCWCVLIDTGRPIPGTSTRYEQPKCDGNARAHPNKPKDLYRSRHLQGCPGSKKNEFLTSVLDALSTDMVHAVTDPAAAGRMPEPDPNHTLEERVVHWYFSQLDKNLSGDIGKKEIKPFRRLLRKKSKPKKCVKKFVEYCDISNDKALSLQELIGCLGVTKEEGAKTSEGTTSSKLNTVKKQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QPZ6</accession>
    <name>A0A2D0QPZ6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zinc finger protein 511</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">znf511</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017319530.1">
        <property type="nucleotide sequence ID" value="XM_017464041.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QPZ6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030572"/>
    <dbReference type="GeneID" id="108263346"/>
    <dbReference type="KEGG" id="ipu:108263346"/>
    <dbReference type="CTD" id="118472"/>
    <dbReference type="OMA" id="LEDYQHH"/>
    <dbReference type="OrthoDB" id="18440at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039258">
        <property type="entry name" value="ZNF511"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21354">
        <property type="entry name" value="ZINC FINGER PROTEIN 511"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21354:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN 511"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017319530.1"/>
        </source>
    </evidence>
    <sequence length="285" mass="32954" checksum="1F2CC18B38F5F3FA" modified="2017-12-20" version="1">MLQSTNLIQLLVGCDAQRIDIHSVPVVKNSSDVYSQQFAKRGDGLEENSSPFNFTPRTIRLDKDEEFFVDGDVHRHLYLQDLVTPLTEESPSLNISEFQCHIAGCKQLFDTLEGYEHHYNSLHRHVCSSCKRSFPSNRLLDIHILEWHDSLFKVMADRECMYQCLVEGCGLKFQTSKKRKEHLITTHSYPSDFRFDKPKKMKRGTKEEKIPQQDEKRLTGPGNVVSRHGELENCELMDFSLTQESESHELALPERQKPHYTYKVPPTICFGHGSVRGFRGGRKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QQD4</accession>
    <name>A0A2D0QQD4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">ATP-sensitive inward rectifier potassium channel 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC124627015</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047009292.1">
        <property type="nucleotide sequence ID" value="XM_047153336.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QQD4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013264"/>
    <dbReference type="GeneID" id="124627015"/>
    <dbReference type="KEGG" id="ipu:124627015"/>
    <dbReference type="OrthoDB" id="273257at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015272">
        <property type="term" value="F:ATP-activated inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1400:FF:000002">
        <property type="entry name" value="ATP-sensitive inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1400">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041647">
        <property type="entry name" value="IRK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016449">
        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003268">
        <property type="entry name" value="K_chnl_inward-rec_Kir1.1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013518">
        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040445">
        <property type="entry name" value="Kir_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF6">
        <property type="entry name" value="ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01007">
        <property type="entry name" value="IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17655">
        <property type="entry name" value="IRK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005465">
        <property type="entry name" value="GIRK_kir"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01321">
        <property type="entry name" value="KIR11CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01320">
        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12 16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0630">Potassium</keyword>
    <keyword evidence="3 12" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 12" id="KW-0813">Transport</keyword>
    <keyword evidence="5 12" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="domain" description="Potassium channel inwardly rectifying transmembrane" evidence="13">
        <location>
            <begin position="24"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="14">
        <location>
            <begin position="173"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_047009292.1"/>
        </source>
    </evidence>
    <sequence length="376" mass="43287" checksum="8367B2458F50F18D" modified="2017-12-20" version="1">MPWSLSQFLRRYVTRRRNKQIRLITKDGHCNIKHGKMKYSTCFAYMHDIWSTCVQIRWYSLTFFYVASFIFSWFIFTLIWYWVGRNNGDLWWQNQTANHSACVLNLYDLKTAYLFSVETQLTIGFGYRVITPYCPSAIAVFMVQVLAGIVIACFWCGVLIAKISLPKKVAKAVTFSETAVICFKQDTLSLQIRVANIRKSLLLGSQIYGKLIRTRVTPEGKTFIMEQVNIDFLVDAGKDNLFFVCPLTLYHVIDKASPFYQMAVDTLHQQDFELVVFLDGTAESTSSSCQVRTSYLPREIIWGYKFLPIVSRSKEGKYRIDFSNFDKVEPVPTAHCAYCYHDLNGHHNFSKDGVDNAGFEVIEIDSQVNQSCSVNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QQX2</accession>
    <name>A0A2D0QQX2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">WD repeat-containing protein 49</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC108264075</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017320868.2">
        <property type="nucleotide sequence ID" value="XM_017465379.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QQX2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003982"/>
    <dbReference type="GeneID" id="108264075"/>
    <dbReference type="KEGG" id="ipu:108264075"/>
    <dbReference type="OrthoDB" id="10251381at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051242">
        <property type="entry name" value="WD-EF-hand_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44324:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44324">
        <property type="entry name" value="WD40 REPEAT DOMAIN 95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="370"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="456"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="507"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="591"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="641"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="833"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="644"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="836"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="850"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="860"/>
            <end position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017320868.2"/>
        </source>
    </evidence>
    <sequence length="972" mass="108540" checksum="5A16894CC1BD8CF4" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QS70</accession>
    <name>A0A2D0QS70_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mothers against decapentaplegic homolog</fullName>
            <shortName evidence="9">MAD homolog</shortName>
            <shortName evidence="9">Mothers against DPP homolog</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">SMAD family member</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">smad1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the dwarfin/SMAD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017320330.1">
        <property type="nucleotide sequence ID" value="XM_017464841.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QS70"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006761"/>
    <dbReference type="GeneID" id="108263743"/>
    <dbReference type="KEGG" id="ipu:108263743"/>
    <dbReference type="CTD" id="4086"/>
    <dbReference type="OMA" id="ENNNRAD"/>
    <dbReference type="OrthoDB" id="5794312at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071144">
        <property type="term" value="C:heteromeric SMAD protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070411">
        <property type="term" value="F:I-SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009953">
        <property type="term" value="P:dorsal/ventral pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009880">
        <property type="term" value="P:embryonic pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060395">
        <property type="term" value="P:SMAD protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10490">
        <property type="entry name" value="MH1_SMAD_1_5_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10497">
        <property type="entry name" value="MH2_SMAD_1_5_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.10:FF:000001">
        <property type="entry name" value="Mothers against decapentaplegic homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.520.10:FF:000001">
        <property type="entry name" value="Mothers against decapentaplegic homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.520.10">
        <property type="entry name" value="SMAD MH1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013790">
        <property type="entry name" value="Dwarfin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003619">
        <property type="entry name" value="MAD_homology1_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013019">
        <property type="entry name" value="MAD_homology_MH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017855">
        <property type="entry name" value="SMAD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001132">
        <property type="entry name" value="SMAD_dom_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036578">
        <property type="entry name" value="SMAD_MH1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703:SF23">
        <property type="entry name" value="MOTHERS AGAINST DECAPENTAPLEGIC HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703">
        <property type="entry name" value="SMAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03165">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03166">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00523">
        <property type="entry name" value="DWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00524">
        <property type="entry name" value="DWB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56366">
        <property type="entry name" value="SMAD MH1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51075">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51076">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MH1" evidence="11">
        <location>
            <begin position="12"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="MH2" evidence="12">
        <location>
            <begin position="278"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="159"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="186"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="211"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017320330.1"/>
        </source>
    </evidence>
    <sequence length="472" mass="53012" checksum="786C9CBEB5CE07EB" modified="2017-12-20" version="1">MNVTSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELERALSCPGQPSSCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKALECCQFPFGSKQKDVCINPYHYKRVDSPVLPPVLVPRNSEFNAKLSMLPRFRNPLHQTEPHMPHNIKYPESFTQQPGNSLPFGPNSPSNSYPGSPNSGTASSATFPHSPASSEPSSPFQMPETPPPAYVPPEEPMTQDCPQPMDTNLLAPNLPVEINNRTDVQSVAYEEPKHWCSIVYYELNNRVGEAFQASSTSVLVDGFTDPSNNRNRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSDSSIFVQSRNCNYHHGFHPTTVCKIPSGCSLKIFNNQEFAELLAQSVNHGFEAVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QSQ8</accession>
    <name>A0A2D0QSQ8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Ras-related protein Rab-23</fullName>
            <ecNumber evidence="7">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29 30 31" type="primary">rab23</name>
    </gene>
    <organism evidence="28 30">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">SDA103</strain>
        </source>
    </reference>
    <reference evidence="29 30 31" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="29 30 31">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="26">Interacts with SUFU.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasmic vesicle</location>
            <location evidence="5">Phagosome membrane</location>
            <topology evidence="5">Lipid-anchor</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="7"/>
    <dbReference type="RefSeq" id="XP_017320519.1">
        <property type="nucleotide sequence ID" value="XM_017465030.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017320520.1">
        <property type="nucleotide sequence ID" value="XM_017465031.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053535457.1">
        <property type="nucleotide sequence ID" value="XM_053679482.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QSQ8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000024420"/>
    <dbReference type="GeneID" id="108263845"/>
    <dbReference type="KEGG" id="ipu:108263845"/>
    <dbReference type="CTD" id="51715"/>
    <dbReference type="GeneTree" id="ENSGT00940000155064"/>
    <dbReference type="OrthoDB" id="6585768at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04106">
        <property type="entry name" value="Rab23_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000669">
        <property type="entry name" value="Ras-related protein Rab-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034114">
        <property type="entry name" value="Rab23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="24" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="16" id="KW-0967">Endosome</keyword>
    <keyword evidence="20" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="22" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="21" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0488">Methylation</keyword>
    <keyword evidence="15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-0636">Prenylation</keyword>
    <keyword evidence="19" id="KW-0653">Protein transport</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00062634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00067823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_017320519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="RefSeq" id="XP_017320520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="RefSeq" id="XP_053535457.1"/>
        </source>
    </evidence>
    <sequence length="236" mass="26841" checksum="C618CEB11699483D" modified="2017-12-20" version="1">MLEEDMEVAIKVVVVGNGAVGKSSMIQRYCKGIFTKDYKKTIGVDFLERQIVVNDEDVRLMLWDTAGQEEFDAITKAYYRGAQACVLVFSTTDRESFEAISSWREKVEMEVGDIPTVLVQNKIDLLDDTLIKTEEAEGLAKKLKLRFYRASVKEDLNVNEVFKYLAEKYLQRLKQQTAEDPVVHTSSNKIGVFNTSSGNQPNQNCSDLNGREVINLRPNKQRTKKNKIPFGSCRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QZY9</accession>
    <name>A0A2D0QZY9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">EF-hand domain-containing protein D2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">efhd2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017323963.1">
        <property type="nucleotide sequence ID" value="XM_017468474.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QZY9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000004345"/>
    <dbReference type="GeneID" id="108265784"/>
    <dbReference type="KEGG" id="ipu:108265784"/>
    <dbReference type="CTD" id="79180"/>
    <dbReference type="OMA" id="ERMFKQY"/>
    <dbReference type="OrthoDB" id="6572480at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000112">
        <property type="entry name" value="EF-hand domain family, member D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040365">
        <property type="entry name" value="EFHD1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13025:SF7">
        <property type="entry name" value="EF-HAND DOMAIN FAMILY, MEMBER D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13025">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="84"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="120"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="201"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="22"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017323963.1"/>
        </source>
    </evidence>
    <sequence length="232" mass="26539" checksum="3BA009188897A1E6" modified="2017-12-20" version="1">MASNELSAKLHRRLKIEEGEEEPHAVDEGHENGAHEKASTANADSELGAKLLRRGELNEGMGEHQQPSMKVFNPYTEFKEFSRKQIKDMEKMFKLYDSEKDNYIDLMELKLMMEKLGAPQTHMGLKNMIKEVDEDLDGKLNFREFLLIFRKAAAGELAEDSGLCVLARLSEIDVSTEGVKGAKSFFEAKVHAINESNRFEAEIRNEQEEKKRQAEEKKQRQAAFKNLQSAFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R2P9</accession>
    <name>A0A2D0R2P9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Sterol 26-hydroxylase, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC108261037</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017317328.1">
        <property type="nucleotide sequence ID" value="XM_017461839.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R2P9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000615"/>
    <dbReference type="GeneID" id="108261037"/>
    <dbReference type="KEGG" id="ipu:108261037"/>
    <dbReference type="OMA" id="KAMLWLK"/>
    <dbReference type="OrthoDB" id="3945418at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006700">
        <property type="term" value="P:C21-steroid hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071375">
        <property type="term" value="P:cellular response to peptide hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034650">
        <property type="term" value="P:cortisol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006704">
        <property type="term" value="P:glucocorticoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042359">
        <property type="term" value="P:vitamin D metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000006">
        <property type="entry name" value="Cytochrome P450 302a1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050479">
        <property type="entry name" value="CYP11_CYP27_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279:SF123">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 27 SUBFAMILY A MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="466"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017317328.1"/>
        </source>
    </evidence>
    <sequence length="531" mass="60688" checksum="0BB6FC37B9EEC3CF" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R483</accession>
    <name>A0A2D0R483_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Abl interactor 2a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">abi2a</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Filopodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ABI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017325389.1">
        <property type="nucleotide sequence ID" value="XM_017469900.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R483"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000017770"/>
    <dbReference type="GeneID" id="108266499"/>
    <dbReference type="KEGG" id="ipu:108266499"/>
    <dbReference type="CTD" id="767646"/>
    <dbReference type="OrthoDB" id="5971719at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030175">
        <property type="term" value="C:filopodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031209">
        <property type="term" value="C:SCAR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11972">
        <property type="entry name" value="SH3_Abi2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000002">
        <property type="entry name" value="abl interactor 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028457">
        <property type="entry name" value="ABI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035726">
        <property type="entry name" value="Abi2_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012849">
        <property type="entry name" value="Abl-interactor_HHR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10460:SF26">
        <property type="entry name" value="ABL INTERACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10460">
        <property type="entry name" value="ABL INTERACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07815">
        <property type="entry name" value="Abi_HHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="14">
        <location>
            <begin position="45"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="413"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="156"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="359"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="168"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="211"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="308"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="319"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017325389.1"/>
        </source>
    </evidence>
    <sequence length="475" mass="52263" checksum="E2E58CDE86EA91E8" modified="2017-12-20" version="1">MAELQMLLEEEIPAGRTALLDSFTNLERVAEYCESNYVQSPDKHRALEETKNYTTQSLASVAYLINTLANNVLQMLDIQASQLRRMESSINHISQTVDIHKEKVARREIGILTTNKNTSRTHKIIAPANPERPVRYIRKPIDYNLLDDIGHGVKVGNQSMKMGGLPRTTPPTQKPPSPPMPGKGTIGRHSPYRTLEPVRPPVIPNDYVPSPTRNMAPGQQQSPVRTASLNQRNRTYSGSSGGSHPSSRSSSRENSGSGSVGVPIAVPTPSPPNAFPGTAPQFYSMNRPVPRQITPPVGGSLPYRRPPSVSSQPSMPASQQNGGPYYNQGQASVAPPPPSILQITPQLPLTGFVARVQETISDAPPPPPPTEESMLQEAQPPPPPLEDYEEEESSVVEYSDPYAEEDPPWAPCAYLEKVVAIYDYTQDKEDELSFQEGAIIYVIKKNDDGWYEGVMNGTTGLFPGNYVESIMHYRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RAS5</accession>
    <name>A0A2D0RAS5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 6 7" type="primary">plscr3b</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5 6 7" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5 6 7">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017327659.2">
        <property type="nucleotide sequence ID" value="XM_017472170.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017327660.2">
        <property type="nucleotide sequence ID" value="XM_017472171.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017327662.2">
        <property type="nucleotide sequence ID" value="XM_017472173.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RAS5"/>
    <dbReference type="GeneID" id="108267765"/>
    <dbReference type="KEGG" id="ipu:108267765"/>
    <dbReference type="CTD" id="405802"/>
    <dbReference type="OrthoDB" id="444338at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF57">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017327659.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017327660.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017327662.2"/>
        </source>
    </evidence>
    <sequence length="317" mass="34795" checksum="581692E765AA2A52" modified="2023-06-28" version="2">MSAPGYPSPQPPYPMTQMGGPVPPYPVGGYGELDPAAPPAGFYMGYQPSPHQPVMNQPGPGGPPQQPIQMSAYGGDAPYSSPSAQTPSVPVGVPPGLEYLTQIDQVLIHQKVELLEAFISFETNNQYEIKNSLGQKIYKAKEKNDCCTRNCCGALRSFDMKIKDNSDREVIRLIRPFRCASCWCPCCMQELEVQAPPGTTIGYVTQDWHPCYPKFSIKGANKETLMKLEGPCLACNCCGDVNFELKGKDGGKSIGRISKQWSGLLKEAFTDSDNFGIQFPLDMDVKMKAVLMGACFLIDFIFFEKVGDSNQRNTVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RD35</accession>
    <name>A0A2D0RD35_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Transcriptional repressor NF-X1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Nuclear transcription factor, X box-binding protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">nfx1</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="23">Blood</tissue>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NFX1 family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <location>
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        <location>
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        <location>
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        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="121"/>
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        <location>
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        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00007269"/>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS51061"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017328453.1"/>
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    </evidence>
    <sequence length="1091" mass="121581" checksum="7D2AA3675A6AAF6E" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RDI9</accession>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
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                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
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                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
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                <person name="Tan S."/>
                <person name="Peatman E."/>
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                <person name="Qin Z."/>
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                <person name="Sonstegard T."/>
                <person name="Feng J."/>
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                <person name="Duke M.V."/>
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                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
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                <person name="Kirby M.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">SDA103</strain>
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        <source>
            <tissue evidence="3">Blood</tissue>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017328602.3"/>
        </source>
    </evidence>
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    <accession>A0A2D0RDY2</accession>
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        <name evidence="9 10" type="primary">LOC108267963</name>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
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                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the threonine synthase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017328100.1">
        <property type="nucleotide sequence ID" value="XM_017472611.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047014724.1">
        <property type="nucleotide sequence ID" value="XM_047158768.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RDY2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005069"/>
    <dbReference type="GeneID" id="108267963"/>
    <dbReference type="KEGG" id="ipu:108267963"/>
    <dbReference type="OrthoDB" id="5203861at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00464">
        <property type="entry name" value="SK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01560">
        <property type="entry name" value="Thr-synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1380.10">
        <property type="entry name" value="Threonine synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00109">
        <property type="entry name" value="Shikimate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031322">
        <property type="entry name" value="Shikimate/glucono_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000623">
        <property type="entry name" value="Shikimate_kinase/TSH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029144">
        <property type="entry name" value="Thr_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037158">
        <property type="entry name" value="Thr_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43515">
        <property type="entry name" value="THREONINE SYNTHASE-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43515:SF1">
        <property type="entry name" value="THREONINE SYNTHASE-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01202">
        <property type="entry name" value="SKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24857">
        <property type="entry name" value="THR4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14821">
        <property type="entry name" value="Thr_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01100">
        <property type="entry name" value="SHIKIMTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Threonine synthase N-terminal" evidence="7">
        <location>
            <begin position="219"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604450-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017328100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_047014724.1"/>
        </source>
    </evidence>
    <sequence length="732" mass="81645" checksum="0308F3017886FFCE" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RFT0</accession>
    <name>A0A2D0RFT0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein LOC108268753 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC108268753</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017329388.1">
        <property type="nucleotide sequence ID" value="XM_017473899.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RFT0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027613"/>
    <dbReference type="GeneID" id="108268753"/>
    <dbReference type="KEGG" id="ipu:108268753"/>
    <dbReference type="OMA" id="AILICKW"/>
    <dbReference type="OrthoDB" id="8947657at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF133">
        <property type="entry name" value="IMMUNE-TYPE RECEPTOR 5 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012903707" evidence="9">
        <location>
            <begin position="18"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="250"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="24"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="136"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017329388.1"/>
        </source>
    </evidence>
    <sequence length="335" mass="36979" checksum="B36C8612A015F49E" modified="2017-12-20" version="1">MALVWILIFALYKTYSSQSVDFDETVSLVSADLGHAVSLQCTLGEKSRNNLILWYQQKLGGIPQAIGMIGMLMAKIILPPFKKTVFQLGITENNISLNILHVTKDDEAMYFCGMARVNVIEFSSGIFLSVRDKAHLDVSVFQSGVSDSVPAGASVTLQCSVLSESRSAELQVLWFRAAPPQSHPQIIYTHHNSSHQCESGSSTHTCVYNFSKNILSLSDTGTYYCAVAVCGKIIFGNGTRVQLEKSANPFLIILSTSLGVCGILITAQAIFICKRTHYKYHSGPEKENATDQSCNVELNYAALHFNKKKAKSGRRKSKQPQDTVYSEVRRFTVTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RG64</accession>
    <name>A0A2D0RG64_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endoplasmic reticulum-Golgi intermediate compartment protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 12 13" type="primary">ergic2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11 12 13" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11 12 13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Plays a role in transport between endoplasmic reticulum and Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Golgi apparatus membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ERGIC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017329537.1">
        <property type="nucleotide sequence ID" value="XM_017474048.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017329540.1">
        <property type="nucleotide sequence ID" value="XM_017474051.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017329541.1">
        <property type="nucleotide sequence ID" value="XM_017474052.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RG64"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008178"/>
    <dbReference type="GeneID" id="108268821"/>
    <dbReference type="KEGG" id="ipu:108268821"/>
    <dbReference type="CTD" id="51290"/>
    <dbReference type="OMA" id="MTNHYLR"/>
    <dbReference type="OrthoDB" id="5541786at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045888">
        <property type="entry name" value="Erv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012936">
        <property type="entry name" value="Erv_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039542">
        <property type="entry name" value="Erv_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984:SF30">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07970">
        <property type="entry name" value="COPIIcoated_ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13850">
        <property type="entry name" value="ERGIC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter N-terminal" evidence="9">
        <location>
            <begin position="13"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter C-terminal" evidence="8">
        <location>
            <begin position="166"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017329537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017329540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017329541.1"/>
        </source>
    </evidence>
    <sequence length="376" mass="42523" checksum="452F0516BC045945" modified="2017-12-20" version="1">MRRLSRKKALNIVKELDAFPKVPESYVETTASGGTVSLIAFTAMALLAFFEFFVYRDTWMKYEYEVDTDFSSKLRINIDITVAMRCQFVGADVLDLAETMVASDGLKYEPVLFELSPQQRLWHRTLLLIQNRLREEHSLQDVLFKNVMRGAPTALPPREDSPTQPLNACRIHGHLYVNKVAGNFHITVGKAIPHPRGHAHLAALVSHDTYNFSHRIDHLSFGEEIPGILNPLDGTEKVCTDHNQMFQYFITIVPTKLKTYKVTTDTHQYSVTERERVINHAAGSHGVSGIFMKYDLSSLMVRVTEQHMPLWQFLVRLCGIIGGIFSTTGMLHRLVGFFVDTICCRFKLGVYKPKQVSVVDGHANSRTPLLSENGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RJR7</accession>
    <name>A0A2D0RJR7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Atrophin-1-interacting protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Membrane-associated guanylate kinase inverted 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="primary">magi2b</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="29">SDA103</strain>
        </source>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="30">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Cilium</location>
            <location evidence="5">Photoreceptor outer segment</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Late endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Photoreceptor inner segment</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Synapse</location>
            <location evidence="21">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017330170.1">
        <property type="nucleotide sequence ID" value="XM_017474681.3"/>
    </dbReference>
    <dbReference type="GeneID" id="108269110"/>
    <dbReference type="KEGG" id="ipu:108269110"/>
    <dbReference type="CTD" id="100001810"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001917">
        <property type="term" value="C:photoreceptor inner segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001750">
        <property type="term" value="C:photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031697">
        <property type="term" value="F:beta-1 adrenergic receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030159">
        <property type="term" value="F:signaling receptor complex adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046332">
        <property type="term" value="F:SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070699">
        <property type="term" value="F:type II activin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06730">
        <property type="entry name" value="PDZ0_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06731">
        <property type="entry name" value="PDZ1_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06732">
        <property type="entry name" value="PDZ2_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06733">
        <property type="entry name" value="PDZ3_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06734">
        <property type="entry name" value="PDZ4_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06735">
        <property type="entry name" value="PDZ5_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000005">
        <property type="entry name" value="Membrane associated guanylate kinase, WW and PDZ domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000012">
        <property type="entry name" value="Membrane associated guanylate kinase, WW and PDZ domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000113">
        <property type="entry name" value="Membrane associated guanylate kinase, WW and PDZ domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000144">
        <property type="entry name" value="Membrane associated guanylate kinase, WW and PDZ domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000001">
        <property type="entry name" value="Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000155">
        <property type="entry name" value="membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000002">
        <property type="entry name" value="Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000003">
        <property type="entry name" value="Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.10">
        <property type="entry name" value="Guanylate Kinase phosphate binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10316">
        <property type="entry name" value="MEMBRANE ASSOCIATED GUANYLATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10316:SF78">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE, WW AND PDZ DOMAIN-CONTAINING PROTEIN 2 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16663">
        <property type="entry name" value="MAGI_u1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0965">Cell junction</keyword>
    <keyword evidence="20" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="19" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="13" id="KW-0967">Endosome</keyword>
    <keyword evidence="30" id="KW-0418">Kinase</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="17" id="KW-0770">Synapse</keyword>
    <keyword evidence="10" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="7" id="KW-0796">Tight junction</keyword>
    <keyword evidence="30" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="17"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="27">
        <location>
            <begin position="108"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="26">
        <location>
            <begin position="304"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="26">
        <location>
            <begin position="368"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="28">
        <location>
            <begin position="446"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="28">
        <location>
            <begin position="634"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="28">
        <location>
            <begin position="804"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="28">
        <location>
            <begin position="932"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="28">
        <location>
            <begin position="1245"/>
            <end position="1327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="203"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="581"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="899"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1022"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1181"/>
            <end position="1221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="25">
        <location>
            <begin position="229"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="1044"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="1085"/>
            <end position="1106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00070827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00079516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00080410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="RefSeq" id="XP_017330170.1"/>
        </source>
    </evidence>
    <sequence length="1381" mass="150182" checksum="1EF19227373C3866" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RK48</accession>
    <name>A0A2D0RK48_ICTPU</name>
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        <submittedName>
            <fullName evidence="5">Protein FAM149A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">fam149a</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM149 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017330521.1">
        <property type="nucleotide sequence ID" value="XM_017475032.3"/>
    </dbReference>
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    <dbReference type="KEGG" id="ipu:108269272"/>
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    <dbReference type="OrthoDB" id="2134133at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
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        <property type="entry name" value="DUF3719"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039630">
        <property type="entry name" value="FAM149"/>
    </dbReference>
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        <property type="entry name" value="AGAP003710-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31997:SF2">
        <property type="entry name" value="PROTEIN FAM149A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12516">
        <property type="entry name" value="DUF3719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="159"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="33"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017330521.1"/>
        </source>
    </evidence>
    <sequence length="675" mass="74682" checksum="736F3D4BBA53AC72" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RM18</accession>
    <name>A0A2D0RM18_ICTPU</name>
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        <recommendedName>
            <fullName evidence="36">Trifunctional enzyme subunit alpha, mitochondrial</fullName>
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            <ecNumber evidence="6">4.2.1.17</ecNumber>
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        <alternativeName>
            <fullName evidence="37">Monolysocardiolipin acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="38">TP-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="45" type="primary">hadhab</name>
    </gene>
    <organism evidence="44 45">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="44" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="44">SDA103</strain>
        </source>
    </reference>
    <reference evidence="45" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="45">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>(3S)-3-hydroxydodecanoyl-CoA + NAD(+) = 3-oxododecanoyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31179"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62558"/>
            <dbReference type="ChEBI" id="CHEBI:62615"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:31180"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>(3S)-3-hydroxydodecanoyl-CoA = (2E)-dodecenoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:31075"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57330"/>
            <dbReference type="ChEBI" id="CHEBI:62558"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="29">
            <dbReference type="Rhea" id="RHEA:31077"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>(3S)-hydroxydecanoyl-CoA + NAD(+) = 3-oxodecanoyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31187"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62548"/>
            <dbReference type="ChEBI" id="CHEBI:62616"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:31188"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(3S)-hydroxyhexadecanoyl-CoA + NAD(+) = 3-oxohexadecanoyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31159"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57349"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62613"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:31160"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(3S)-hydroxyhexadecanoyl-CoA = (2E)-hexadecenoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:31163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
            <dbReference type="ChEBI" id="CHEBI:62613"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="1">
            <dbReference type="Rhea" id="RHEA:31165"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>(3S)-hydroxyoctanoyl-CoA + NAD(+) = 3-oxooctanoyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31195"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62617"/>
            <dbReference type="ChEBI" id="CHEBI:62619"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:31196"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>(3S)-hydroxyoctanoyl-CoA = (2E)-octenoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:31199"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:62242"/>
            <dbReference type="ChEBI" id="CHEBI:62617"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="27">
            <dbReference type="Rhea" id="RHEA:31201"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>(3S)-hydroxytetradecanoyl-CoA + NAD(+) = 3-oxotetradecanoyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62543"/>
            <dbReference type="ChEBI" id="CHEBI:62614"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:31168"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>1'-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-3'-[1-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-glycerol + (9Z)-octadecenoyl-CoA = 1'-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-3'-[1-(9Z,12Z-octadecadienoyl)-2-(9Z-octadecenoyl)-sn-glycero-3-phospho]-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:43676"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:83580"/>
            <dbReference type="ChEBI" id="CHEBI:83582"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:43677"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>1'-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-3'-[1-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-glycerol + (9Z,12Z)-octadecadienoyl-CoA = 1',3'-bis-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:43672"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57383"/>
            <dbReference type="ChEBI" id="CHEBI:83580"/>
            <dbReference type="ChEBI" id="CHEBI:83581"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:43673"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>1'-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-3'-[1-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-glycerol + hexadecanoyl-CoA = 1'-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-3'-[1-(9Z,12Z-octadecadienoyl)-2-hexadecanoyl-sn-glycero-3-phospho]-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:43680"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:83580"/>
            <dbReference type="ChEBI" id="CHEBI:83583"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:43681"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:16105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57318"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="EC" id="4.2.1.17"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="21">
            <dbReference type="Rhea" id="RHEA:16107"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:20724"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58521"/>
            <dbReference type="ChEBI" id="CHEBI:137480"/>
            <dbReference type="EC" id="4.2.1.17"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="26">
            <dbReference type="Rhea" id="RHEA:20726"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>a long-chain (3S)-3-hydroxy fatty acyl-CoA + NAD(+) = a long-chain 3-oxo-fatty acyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52656"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:136757"/>
            <dbReference type="ChEBI" id="CHEBI:136758"/>
            <dbReference type="EC" id="1.1.1.211"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:52657"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="34">Heterotetramer of 2 alpha/HADHA and 2 beta/HADHB subunits; forms the mitochondrial trifunctional enzyme. Also purified as higher order heterooligomers including a 4 alpha/HADHA and 4 beta/HADHB heterooligomer which physiological significance remains unclear. The mitochondrial trifunctional enzyme interacts with MTLN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="41">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.211" evidence="35"/>
    <dbReference type="EC" id="4.2.1.17" evidence="6"/>
    <dbReference type="RefSeq" id="XP_017331191.1">
        <property type="nucleotide sequence ID" value="XM_017475702.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RM18"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035205"/>
    <dbReference type="GeneID" id="100304971"/>
    <dbReference type="KEGG" id="ipu:100304971"/>
    <dbReference type="CTD" id="793834"/>
    <dbReference type="OMA" id="ESTTIRW"/>
    <dbReference type="OrthoDB" id="10004768at2759"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016507">
        <property type="term" value="C:mitochondrial fatty acid beta-oxidation multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016509">
        <property type="term" value="F:long-chain (3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000011">
        <property type="entry name" value="Fatty acid oxidation complex subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000009">
        <property type="entry name" value="Fatty oxidation complex, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.50:FF:000002">
        <property type="entry name" value="Trifunctional enzyme subunit alpha, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006180">
        <property type="entry name" value="3-OHacyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012803">
        <property type="entry name" value="Fa_ox_alpha_mit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050136">
        <property type="entry name" value="FA_oxidation_alpha_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02441">
        <property type="entry name" value="fa_ox_alpha_mit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43612">
        <property type="entry name" value="TRIFUNCTIONAL ENZYME SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43612:SF3">
        <property type="entry name" value="TRIFUNCTIONAL ENZYME SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00067">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="19" id="KW-0456">Lyase</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0488">Methylation</keyword>
    <keyword evidence="17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="20" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="15" id="KW-0520">NAD</keyword>
    <keyword evidence="14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="44" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase NAD binding" evidence="43">
        <location>
            <begin position="365"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="42">
        <location>
            <begin position="545"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="active site" description="For hydroxyacyl-coenzyme A dehydrogenase activity" evidence="39">
        <location>
            <position position="511"/>
        </location>
    </feature>
    <feature type="site" description="Important for long-chain enoyl-CoA hydratase activity" evidence="40">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="site" description="Important for long-chain enoyl-CoA hydratase activity" evidence="40">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="site" description="Important for hydroxyacyl-coenzyme A dehydrogenase activity" evidence="40">
        <location>
            <position position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00035854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00050222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00050446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00051215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00051877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00052224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00052711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00052834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00052860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00052945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00052989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00062153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00066806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00068347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00077617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00083277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="PIRSR" id="PIRSR612803-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="PIRSR" id="PIRSR612803-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="42">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="43">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="44">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="45">
        <source>
            <dbReference type="RefSeq" id="XP_017331191.1"/>
        </source>
    </evidence>
    <sequence length="764" mass="82811" checksum="66084ED87E0FB90F" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RPL1</accession>
    <name>A0A2D0RPL1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Protein FAM13A isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">LOC108270362</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5 6">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM13 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017332443.2">
        <property type="nucleotide sequence ID" value="XM_017476954.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047013812.2">
        <property type="nucleotide sequence ID" value="XM_047157856.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RPL1"/>
    <dbReference type="GeneID" id="108270362"/>
    <dbReference type="KEGG" id="ipu:108270362"/>
    <dbReference type="OrthoDB" id="185175at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039102">
        <property type="entry name" value="FAM13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059029">
        <property type="entry name" value="FAM13A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15904">
        <property type="entry name" value="FAM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15904:SF19">
        <property type="entry name" value="PROTEIN FAM13C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26116">
        <property type="entry name" value="FAM13A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="42"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="234"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="441"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="511"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="706"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="241"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="254"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="478"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="512"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="550"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="581"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="597"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="639"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="706"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="727"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017332443.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_047013812.2"/>
        </source>
    </evidence>
    <sequence length="967" mass="108637" checksum="61D23764F7B342F5" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RQT1</accession>
    <name>A0A2D0RQT1_ICTPU</name>
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        <submittedName>
            <fullName evidence="10">Myocyte-specific enhancer factor 2B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">mef2b</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017332868.1">
        <property type="nucleotide sequence ID" value="XM_017477379.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RQT1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000924"/>
    <dbReference type="GeneID" id="108270554"/>
    <dbReference type="KEGG" id="ipu:108270554"/>
    <dbReference type="CTD" id="100271849"/>
    <dbReference type="OMA" id="TAFMSPH"/>
    <dbReference type="OrthoDB" id="1898716at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000001">
        <property type="entry name" value="Myocyte-specific enhancer factor 2A homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945">
        <property type="entry name" value="MADS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945:SF145">
        <property type="entry name" value="MYOCYTE ENHANCER FACTOR 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="8">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="332"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="168"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="371"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017332868.1"/>
        </source>
    </evidence>
    <sequence length="386" mass="42169" checksum="74A2795FFADB4114" modified="2017-12-20" version="1">MGRKKIQISRILDQRNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFNSTNRLFQYASTDMDKVLLKYTEYSEPHESRTNNDILETLRRKGLGLDGAELEHEDNMQTGQEKYLLNEGMDLSIARQRYYAPSVHPSEAQMLMGVGCENGYPSNMNLGANRPSSFKPLSSRPGSASPAGANTHSALMGTHAGFGYSVFSHGNLSRALDMKTPPPLSLTNDGRRGEVHPGVGVARGNLTNARALYQSMHSGGHMASTGKPWNYGTSEFCHPGYTVGFQRRSANTWQPLTQQEPQPSVIHAGMSTGGGCFYSSQGSSPPSPPLSSLKLNIKSECVSPEHTCSPGTPSSLHSPVGGPDGMSQPPGPYVDMDREESETNLKHVEVGDGWQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RR66</accession>
    <name>A0A2D0RR66_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ttbk2a</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017332670.1">
        <property type="nucleotide sequence ID" value="XM_017477181.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RR66"/>
    <dbReference type="GeneID" id="108270457"/>
    <dbReference type="KEGG" id="ipu:108270457"/>
    <dbReference type="CTD" id="100331590"/>
    <dbReference type="OrthoDB" id="5979581at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000167">
        <property type="entry name" value="Tau tubulin kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000358">
        <property type="entry name" value="Tau tubulin kinase 2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="21"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="351"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="555"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="664"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="780"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="852"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1080"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="555"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="588"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="696"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="784"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="871"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="928"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="958"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1090"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1120"/>
            <end position="1133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017332670.1"/>
        </source>
    </evidence>
    <sequence length="1165" mass="128279" checksum="F5C7544885B786FB" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RRK5</accession>
    <name>A0A2D0RRK5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Suppressor of cytokine signaling 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">socs7</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Substrate-recognition component of a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex, also named CRL5 complex), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as DAB1 and IRS1. Specifically recognizes and binds phosphorylated proteins via its SH2 domain, promoting their ubiquitination. The ECS(SOCS7) complex acts as a key regulator of reelin signaling by mediating ubiquitination and degradation of phosphorylated DAB1 in the cortical plate of the developing cerebral cortex, thereby regulating neuron positioning during cortex development. Functions in insulin signaling and glucose homeostasis through IRS1 ubiquitination and subsequent proteasomal degradation. Also inhibits prolactin, growth hormone and leptin signaling by preventing STAT3 and STAT5 activation, sequestering them in the cytoplasm and reducing their binding to DNA.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Substrate-recognition component of the ECS(SOCS7) complex, composed of SOCS7, CUL5, ELOB, ELOC and RNF7/RBX2. Interacts, via the third proline-rich region, with the second SH3 domain of the adapter protein NCK1. Also interacts with GRB2, INSR, PLCG1, SORBS3/vinexin, and phosphorylated STAT3 and STAT5. Interacts with SEPT6. Interacts with phosphorylated IRS4 and PIK3R1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017333138.1">
        <property type="nucleotide sequence ID" value="XM_017477649.3"/>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Liu S."/>
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                <person name="Li Y."/>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13 14">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the gamma-tubulin ring complex (gTuRC) which mediates microtubule nucleation. The gTuRC regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments, a critical step in centrosome duplication and spindle formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the gamma-tubulin ring complex (gTuRC) consisting of TUBGCP2, TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6 and gamma-tubulin TUBG1 or TUBG2. TUBGCP2, TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6 assemble in a 5:5:2:1:1 stoichiometry; each is associated with a gamma-tubulin, thereby arranging 14 gamma-tubulins in a helical manner. Gamma-tubulin at the first position is blocked by TUBGCP3 at the last position, allowing 13 protafilaments to grow into a microtubule. The gTuRC (via TUBGCP3 and TUBGCP6) interacts with ACTB and MZT1; the interactions form a luminal bridge that stabilizes the initial structure during complex assembly. The gTuRC (via TUBGCP2) interacts with MZT2A/MZT2B and CDK5RAP2 (via CM1 motif); the interactions play a role in gTuRC activation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017335224.1">
        <property type="nucleotide sequence ID" value="XM_017479735.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017335225.1">
        <property type="nucleotide sequence ID" value="XM_017479736.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RZ43"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033362"/>
    <dbReference type="GeneID" id="108271859"/>
    <dbReference type="KEGG" id="ipu:108271859"/>
    <dbReference type="CTD" id="114791"/>
    <dbReference type="OrthoDB" id="66546at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22572">
        <property type="entry name" value="GCP5_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1900:FF:000005">
        <property type="entry name" value="Gamma-tubulin complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059169">
        <property type="entry name" value="GCP5_N_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF33">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="11">
        <location>
            <begin position="270"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="10">
        <location>
            <begin position="722"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="121"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="338"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="532"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="159"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="347"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00093416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017335224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017335225.1"/>
        </source>
    </evidence>
    <sequence length="1021" mass="117885" checksum="A5730D60890CAC73" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S0Q6</accession>
    <name>A0A2D0S0Q6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein il11b isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">il11b</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017336317.1">
        <property type="nucleotide sequence ID" value="XM_017480828.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S0Q6"/>
    <dbReference type="GeneID" id="100379165"/>
    <dbReference type="KEGG" id="ipu:100379165"/>
    <dbReference type="CTD" id="108180095"/>
    <dbReference type="OrthoDB" id="8828755at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020438">
        <property type="entry name" value="IL-11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16922">
        <property type="entry name" value="INTERLEUKIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16922:SF0">
        <property type="entry name" value="INTERLEUKIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07400">
        <property type="entry name" value="IL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013311190" evidence="1">
        <location>
            <begin position="26"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017336317.1"/>
        </source>
    </evidence>
    <sequence length="205" mass="23792" checksum="C407323A1CF900B2" modified="2017-12-20" version="1">MKLLPESSCVLFLLLLVELPVLAMSRPTPMRHRKDSLTKLSQKMRRLINIVQRAVHHLDTSLPFEHNLTSLPTLNFRPRDLAAVTENSTLSQLSSGLHSFKLHFDWLLYWHNQSGLVSNKIKEIADEIQSISMLEQIQTNSSGQNTLLSMPPLTSAWDIYRTSAVIHKRLLDFCNWYLRALQVLIYKQTTDDKRGHYQEEGHRYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S238</accession>
    <name>A0A2D0S238_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tropomyosin alpha-1 chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Alpha-tropomyosin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Tropomyosin-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">tpm3</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">Homodimer. Heterodimer of an alpha (TPM1, TPM3 or TPM4) and a beta (TPM2) chain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the tropomyosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017336823.1">
        <property type="nucleotide sequence ID" value="XM_017481334.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S238"/>
    <dbReference type="GeneID" id="100305075"/>
    <dbReference type="KEGG" id="ipu:100305075"/>
    <dbReference type="CTD" id="7170"/>
    <dbReference type="OrthoDB" id="128924at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000005">
        <property type="entry name" value="Tropomyosin alpha-1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000001">
        <property type="entry name" value="Tropomyosin alpha-1 chain isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000001">
        <property type="entry name" value="Tropomyosin alpha-1 chain isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000533">
        <property type="entry name" value="Tropomyosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19269">
        <property type="entry name" value="TROPOMYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00261">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00194">
        <property type="entry name" value="TROPOMYOSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00326">
        <property type="entry name" value="TROPOMYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="15"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="284"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="26"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="290"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017336823.1"/>
        </source>
    </evidence>
    <sequence length="337" mass="39002" checksum="4F7B0C6FD283067B" modified="2017-12-20" version="1">MEAVKKKMMMLKLDKENALDQAEQAETDRKAAEERSKQHEDELIQMQKKLKSTEDELDKYSEALKDAQEKLEIAEKKAADAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIVEGELERTEERAELAEAKKKQLEEELRVLDQTLKTLHASENKYSQKEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEDELYAQKLKYKAISEELDHALNDMTSIKPKRCQGGERQDPCHSGPDPERTEQFLNILDEDIPPFPFSTHSSHVSNLVQIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S4B5</accession>
    <name>A0A2D0S4B5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Myosin-9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Cellular myosin heavy chain, type A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Myosin heavy chain 9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Myosin heavy chain, non-muscle IIa</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Non-muscle myosin heavy chain A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Non-muscle myosin heavy chain IIa</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">myh9b</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">SDA103</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="27">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Cytoplasmic vesicle</location>
            <location evidence="15">Secretory vesicle</location>
            <location evidence="15">Cortical granule</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 22">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017337060.1">
        <property type="nucleotide sequence ID" value="XM_017481571.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000031654"/>
    <dbReference type="GeneID" id="108272807"/>
    <dbReference type="KEGG" id="ipu:108272807"/>
    <dbReference type="CTD" id="561431"/>
    <dbReference type="OMA" id="DVRFLHK"/>
    <dbReference type="OrthoDB" id="10254995at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060473">
        <property type="term" value="C:cortical granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030029">
        <property type="term" value="P:actin filament-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01377">
        <property type="entry name" value="MYSc_class_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.360:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1590:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000002">
        <property type="entry name" value="Myosin heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000002">
        <property type="entry name" value="Myosin heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000002">
        <property type="entry name" value="Myosin heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000003">
        <property type="entry name" value="Myosin heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000008">
        <property type="entry name" value="Myosin heavy chain 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000175">
        <property type="entry name" value="Myosin heavy chain 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000007">
        <property type="entry name" value="Myosin heavy chain, non-muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.270.10:FF:000001">
        <property type="entry name" value="Myosin heavy chain, non-muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000009">
        <property type="entry name" value="myosin-11 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.270.10">
        <property type="entry name" value="Myosin, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615">
        <property type="entry name" value="MYOSIN HEAVY CHAIN, NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45615:SF16">
        <property type="entry name" value="MYOSIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 22" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="8" id="KW-0133">Cell shape</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11 22" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10 22" id="KW-0518">Myosin</keyword>
    <keyword evidence="5 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="25">
        <location>
            <begin position="25"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="24">
        <location>
            <begin position="79"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="22">
        <location>
            <begin position="650"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1689"/>
            <end position="1708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1856"/>
            <end position="1914"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1931"/>
            <end position="1954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="1689"/>
            <end position="1700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="1856"/>
            <end position="1865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="1943"/>
            <end position="1954"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <begin position="172"/>
            <end position="179"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00039816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00043098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00079648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00083480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_017337060.1"/>
        </source>
    </evidence>
    <sequence length="1954" mass="227280" checksum="20E97207CCE8B22F" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S907</accession>
    <name>A0A2D0S907_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial import receptor subunit TOM22 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Translocase of outer membrane 22 kDa subunit homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">tomm22</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Central receptor component of the translocase of the outer membrane of mitochondria (TOM complex) responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with the peripheral receptor TOM20 functions as the transit peptide receptor and facilitates the movement of preproteins into the translocation pore. Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom22 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017339229.1">
        <property type="nucleotide sequence ID" value="XM_017483740.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S907"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000002191"/>
    <dbReference type="GeneID" id="108273994"/>
    <dbReference type="KEGG" id="ipu:108273994"/>
    <dbReference type="CTD" id="56993"/>
    <dbReference type="OMA" id="DKDSGME"/>
    <dbReference type="OrthoDB" id="10016939at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001889">
        <property type="term" value="P:liver development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd22884">
        <property type="entry name" value="TOM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005683">
        <property type="entry name" value="Tom22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12504">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12504:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM22 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04281">
        <property type="entry name" value="Tom22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017339229.1"/>
        </source>
    </evidence>
    <sequence length="133" mass="14414" checksum="06045B0F365F5814" modified="2017-12-20" version="1">MAAVSALSPDSGPVETRPPEDDIEGDDEDDLDETLLERLWGLTEMFPDTLRSAAEASAQCSLSLAKKVYSFSRTALWIGTTSFMILVLPVVFETERLQLEQQQLQQQRQILLGPNAGISGVIPGMAPVAPGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SDP8</accession>
    <name>A0A2D0SDP8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sperm axonemal maintenance protein CFAP97D1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">si:ch211-284k5.2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CFAP97 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017340380.1">
        <property type="nucleotide sequence ID" value="XM_017484891.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SDP8"/>
    <dbReference type="GeneID" id="108274663"/>
    <dbReference type="KEGG" id="ipu:108274663"/>
    <dbReference type="OMA" id="MHKSYQP"/>
    <dbReference type="OrthoDB" id="2163395at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038792">
        <property type="entry name" value="CFAP97D1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029488">
        <property type="entry name" value="Hmw/CFAP97"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33768">
        <property type="entry name" value="MIP11318P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33768:SF6">
        <property type="entry name" value="SI:CH211-284K5.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13879">
        <property type="entry name" value="Hmw_CFAP97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="166"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="185"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017340380.1"/>
        </source>
    </evidence>
    <sequence length="195" mass="23035" checksum="FA8BB42FAC3D2E35" modified="2017-12-20" version="1">MHKAYQPLKLAANKYLQNKWDQEHYEEHRKKVREAKPIIDTKGIRTPTHIQVKLKKAQVQEERQAIIHRDNQLLASKLADIQHSKGRIDHRNFYPVRSLNSERRRAELLQVTHENRTIYERITAQESEYRRELWEENWAKTEQRRDGITCYPRGVADKQKVKKSVKFLGGAPGRTLSSSSRTEDGETTDEDLQHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SDV3</accession>
    <name>A0A2D0SDV3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Tripartite motif-containing protein 16 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">zgc:195001</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017340907.3">
        <property type="nucleotide sequence ID" value="XM_017485418.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SDV3"/>
    <dbReference type="GeneID" id="108274956"/>
    <dbReference type="KEGG" id="ipu:108274956"/>
    <dbReference type="OrthoDB" id="8719929at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF80">
        <property type="entry name" value="TRIPARTITE MOTIF-CONTAINING PROTEIN 16-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="5">
        <location>
            <begin position="23"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017340907.3"/>
        </source>
    </evidence>
    <sequence length="217" mass="24200" checksum="D3F37ED601C6AAB5" modified="2023-06-28" version="3">MPELKNSGRKGSKAEARAEQPPYEADIPEPTTRAELIKYWIPLSLDDRTAQKLLWISEGGAKVSRMSEQPCPVLDRPERYEHAPQVVCKDGLLGRRGYWEVEYEGWVVIGVVYASSGRKAKDGACGLGENEASWAVGWGGSHYQAWHAGDSVEIHGNRGNTIGVYVDQPAGVVAFYLLEGEPREARLLHRYKTCFTDELLPGVWVGQKSSCRIRKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SEM6</accession>
    <name>A0A2D0SEM6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc transporter SLC39A7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Solute carrier family 39 member 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Zrt-, Irt-like protein 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">slc39a7</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Zn(2+)(in) = Zn(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29351"/>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">cis-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ZIP transporter (TC 2.A.5) family. KE4/Catsup subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017340695.2">
        <property type="nucleotide sequence ID" value="XM_017485206.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SEM6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006752"/>
    <dbReference type="GeneID" id="108274813"/>
    <dbReference type="KEGG" id="ipu:108274813"/>
    <dbReference type="CTD" id="7922"/>
    <dbReference type="OrthoDB" id="200954at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002395">
        <property type="entry name" value="Kininogen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16950:SF25">
        <property type="entry name" value="ZINC TRANSPORTER SLC39A7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16950">
        <property type="entry name" value="ZINC TRANSPORTER SLC39A7 HISTIDINE-RICH MEMBRANE PROTEIN KE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00334">
        <property type="entry name" value="KININOGEN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0864">Zinc transport</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039920759" description="Zinc transporter SLC39A7" evidence="14">
        <location>
            <begin position="19"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="430"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="455"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="490"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="21"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="83"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="314"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="21"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="83"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="160"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="177"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="314"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="327"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00043053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017340695.2"/>
        </source>
    </evidence>
    <sequence length="511" mass="55302" checksum="A11F01A659EB14FA" modified="2023-06-28" version="2">MRALCVLLLVFGLAVLSCEHGHHHGHHHDHHHGHHHGHHHGHHHGHHHGHHHGHHHGHHHDDVKLFHGASKWSAEANLPPEEELLHDHGHDDGHDHGHDDGHDHGHDDHGHDHDQDHGHAHDHGHDHGHAHDHGHDHGHAHDHGQDHGHAHDHGHDRGHTHNHGHAHGHTHKPMHSPGKERMKHESQAESRDSVQLWMQAVGATLLISAAPFLILFLIPVQSNSDQHQNLLKILLSFASGGLLGDAFLHLIPHALAPHSHHGNEGQSAAAEESHGHSHGVAHDHMMSVGLWVLGGIVAFLVVEKFVRLLKGGHSHSHSHAHSHAAPKCKDSDGEEEEKKKEGGKKDQKKDKKVKKVKEESSDIKVSGYLNLAADFTHNFTDGLAIGASFLVSPAVGVVTTITILLHEVPHEIGDFAILVQSGCTKKKAMCLQLLTAIGALAGTACSLLAEGVGAAATAWILPFTAGGFVYIATVTVLPELLSGRSSAGQSVMEVAAMLFGVGMMVIIAEYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SGM1</accession>
    <name>A0A2D0SGM1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CaM kinase-like vesicle-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">camkvl</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Does not appear to have detectable kinase activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with calmodulin, in the presence of calcium.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017341868.1">
        <property type="nucleotide sequence ID" value="XM_017486379.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SGM1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026664"/>
    <dbReference type="GeneID" id="108275516"/>
    <dbReference type="KEGG" id="ipu:108275516"/>
    <dbReference type="CTD" id="553431"/>
    <dbReference type="OMA" id="EIMILKV"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000188">
        <property type="entry name" value="CaM kinase-like vesicle-associated, like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000155">
        <property type="entry name" value="CaM kinase-like vesicle-associated, like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="24"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="333"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="490"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="547"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="669"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="346"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="402"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="506"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="555"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="692"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="724"/>
            <end position="733"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017341868.1"/>
        </source>
    </evidence>
    <sequence length="761" mass="84531" checksum="CCD578D60F78FBF3" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SWP8</accession>
    <name>A0A2D0SWP8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">A kinase (PRKA) anchor protein 1b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">akap1b</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017346951.1">
        <property type="nucleotide sequence ID" value="XM_017491462.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SWP8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005293"/>
    <dbReference type="GeneID" id="108278245"/>
    <dbReference type="KEGG" id="ipu:108278245"/>
    <dbReference type="CTD" id="556208"/>
    <dbReference type="OMA" id="EHATATC"/>
    <dbReference type="OrthoDB" id="10069557at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034237">
        <property type="term" value="F:protein kinase A regulatory subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22395">
        <property type="entry name" value="KH-I_AKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20407">
        <property type="entry name" value="Tudor_AKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047368">
        <property type="entry name" value="KH-I_AKAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047367">
        <property type="entry name" value="Tudor_AKAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050621">
        <property type="entry name" value="Tudor_domain_containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948:SF65">
        <property type="entry name" value="A-KINASE ANCHORING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948">
        <property type="entry name" value="TUDOR DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="4">
        <location>
            <begin position="723"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="239"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="527"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="254"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="537"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017346951.1"/>
        </source>
    </evidence>
    <sequence length="865" mass="93334" checksum="F9FFBF8373EDC8BC" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SWW1</accession>
    <name>A0A2D0SWW1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Na(+)/H(+) exchange regulatory cofactor NHE-RF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC108278278</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Endomembrane system</location>
            <topology evidence="1 4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017347016.1">
        <property type="nucleotide sequence ID" value="XM_017491527.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SWW1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013415"/>
    <dbReference type="GeneID" id="108278278"/>
    <dbReference type="KEGG" id="ipu:108278278"/>
    <dbReference type="OMA" id="MDPFAEM"/>
    <dbReference type="OrthoDB" id="10007415at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06768">
        <property type="entry name" value="PDZ_NHERF-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000068">
        <property type="entry name" value="Na(+)/H(+) exchange regulatory cofactor NHE-RF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015098">
        <property type="entry name" value="EBP50_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051067">
        <property type="entry name" value="NHER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017300">
        <property type="entry name" value="NHERF-1/NHERF-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14191:SF4">
        <property type="entry name" value="NA(+)_H(+) EXCHANGE REGULATORY COFACTOR NHE-RF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14191">
        <property type="entry name" value="PDZ DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09007">
        <property type="entry name" value="EBP50_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037866">
        <property type="entry name" value="EBP50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="9"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="172"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="108"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="259"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="337"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR037866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017347016.1"/>
        </source>
    </evidence>
    <sequence length="361" mass="39956" checksum="ECE339D8160FB8B6" modified="2017-12-20" version="1">MASDLKPRLCCMTKGENGYGFHLHGEKGKTGQYIRKVEHPSPAEESGLRAGDRVVEVNGENVERETHQQVVQRIKAMEHETRLLVVDRETDEYLRSLRLTCTEDMAVRVSPSASSSSPPPTHSKRENNSVSKLPTSREIPKPSRRSPSRATKKEAPVMPSVPADVSELVPRLCHLVRAETGYGFNLHSEKSRPGQYIRSLDPSSPADRAGLRPQDRLIEVNGVNIEGMRHAEVVAFIKRGGDETHLLVVDPDTDEHFKKLGITPSSSHVKGFEGQTITNGSPNPRVNGHSTQSAHSDHSSQDTSTQNSEDGDSHSLDPFAEIGLRLSPTAAEAKEKAHAKRGKKRAPQMDWNKKYEIFSNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SXJ3</accession>
    <name>A0A2D0SXJ3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">CUGBP Elav-like family member 4 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">celf4</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CELF/BRUNOL family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347403.1">
        <property type="nucleotide sequence ID" value="XM_017491914.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SXJ3"/>
    <dbReference type="GeneID" id="108278496"/>
    <dbReference type="KEGG" id="ipu:108278496"/>
    <dbReference type="CTD" id="56853"/>
    <dbReference type="OrthoDB" id="410044at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12632">
        <property type="entry name" value="RRM1_CELF3_4_5_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12635">
        <property type="entry name" value="RRM2_CELF3_4_5_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12639">
        <property type="entry name" value="RRM3_CELF3_4_5_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000007">
        <property type="entry name" value="CUGBP Elav-like family member 4 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000010">
        <property type="entry name" value="CUGBP Elav-like family member 4 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000069">
        <property type="entry name" value="CUGBP Elav-like family member 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034648">
        <property type="entry name" value="CELF3/4/5/6_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="92"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="180"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="481"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017347403.1"/>
        </source>
    </evidence>
    <sequence length="566" mass="60496" checksum="27334708591A123A" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SXS8</accession>
    <name>A0A2D0SXS8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Large ribosomal subunit protein mL44</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">39S ribosomal protein L44, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">mrpl44</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the 39S subunit of mitochondrial ribosome. May have a function in the assembly/stability of nascent mitochondrial polypeptides exiting the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ribonuclease III family. Mitochondrion-specific ribosomal protein mL44 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347125.1">
        <property type="nucleotide sequence ID" value="XM_017491636.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SXS8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027549"/>
    <dbReference type="GeneID" id="108278331"/>
    <dbReference type="KEGG" id="ipu:108278331"/>
    <dbReference type="CTD" id="65080"/>
    <dbReference type="OMA" id="RHIKRWV"/>
    <dbReference type="OrthoDB" id="444135at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070877">
        <property type="term" value="C:microprocessor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070125">
        <property type="term" value="P:mitochondrial translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19874">
        <property type="entry name" value="DSRM_MRPL44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000010">
        <property type="entry name" value="39S ribosomal protein L44, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000037">
        <property type="entry name" value="39S ribosomal protein L44, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044444">
        <property type="entry name" value="Ribosomal_mL44_DSRM_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055189">
        <property type="entry name" value="RM44_endonuclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207">
        <property type="entry name" value="RIBONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22892">
        <property type="entry name" value="DSRM_MRPL44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22935">
        <property type="entry name" value="RM44_endonuclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 18" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Large ribosomal subunit protein mL44 endonuclease" evidence="16">
        <location>
            <begin position="67"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein mL44 dsRNA binding" evidence="15">
        <location>
            <begin position="232"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="317"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00059807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF22892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF22935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017347125.1"/>
        </source>
    </evidence>
    <sequence length="335" mass="37619" checksum="52FBB4A66C64DF22" modified="2017-12-20" version="1">MAACRTLVSRGVLTLGLHYRHVCRSVLVTQSREKKRWMKAYTLLMERKRRLEGLPPPTPRSQQPNWDYHAEVEAFSARLKEHFSTDLLKMAFVNPCYVRSEEERRRSLGLDAVSTALNLRDNAELQEHGRNFTLGFLADWCRGTFPSLPEDGVAAIVCHLTGLEVMCYVARNLAVEELAMTAEFPVPDDILRETFFAVIGALEQSSGSERAGLFIRDFLVTQLIGKDLFDMWKVVNPMGILVEELSRRGASLPEPRLIQSSGASTVLPLYFVGLYCDKKLLAHGPGETLSAAEEEAARVALRKLFGYTENRKPLDFSTKQEQPETSAVKQAVSRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SYX5</accession>
    <name>A0A2D0SYX5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">POU domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">pou4f3</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the POU transcription factor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347540.1">
        <property type="nucleotide sequence ID" value="XM_017492051.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SYX5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018423"/>
    <dbReference type="GeneID" id="108278602"/>
    <dbReference type="KEGG" id="ipu:108278602"/>
    <dbReference type="CTD" id="5459"/>
    <dbReference type="OrthoDB" id="6358449at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000056">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000007">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF70">
        <property type="entry name" value="INHIBITORY POU PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00157">
        <property type="entry name" value="Pou"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00028">
        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00352">
        <property type="entry name" value="POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00035">
        <property type="entry name" value="POU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00465">
        <property type="entry name" value="POU_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="POU-specific" evidence="12">
        <location>
            <begin position="170"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="265"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="267"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="82"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="152"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017347540.1"/>
        </source>
    </evidence>
    <sequence length="331" mass="36034" checksum="E0426729E446BB41" modified="2017-12-20" version="1">MNGKQQYFSVHAPVHEPKYSSVHTSSEAMRRVCLPAPQLQANIFGAFDESLLARAEALAAADLAKPHATPYHAMNGGVCATSASSSSISSSSSHHHHHHHHHQLSQTLEGELLEHLSSALAVGGLAPNPSALAHQHHLPAVACHLHQATGHAGLPHAHSHPHLPLLPGGDGDSDPRELEAFAERFKQRRIKLGVTQADVGSALANLKIPGVGSLSQSTICRFESLTLSHNNMIALRPVLQAWLDEAEAAHRDTSARTDLMLNGNGERKRKRTSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLELKKNVVRVWFCNQRQKQKRMKYSAAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T038</accession>
    <name>A0A2D0T038_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Prefoldin subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pfdn1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017348146.1">
        <property type="nucleotide sequence ID" value="XM_017492657.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T038"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035887"/>
    <dbReference type="GeneID" id="100304768"/>
    <dbReference type="KEGG" id="ipu:100304768"/>
    <dbReference type="CTD" id="5201"/>
    <dbReference type="OMA" id="REMIQQK"/>
    <dbReference type="OrthoDB" id="5242628at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23164">
        <property type="entry name" value="Prefoldin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20903:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20903">
        <property type="entry name" value="PREFOLDIN SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="93"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017348146.1"/>
        </source>
    </evidence>
    <sequence length="142" mass="16252" checksum="A349A850D6674C64" modified="2017-12-20" version="1">MNAEGNQQCACAFVLPVSSNMAAPVDLELKKAFAELQAKMIDTQQKVKLADLQIEQLSRMKKHANLTHVEITSLPNNTRVYEGVGRMFILQSKDEINNQLTEKQKTADDKIKELEQKKTYLERSVKEAEDNIREMLMSRRAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T0P7</accession>
    <name>A0A2D0T0P7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nuclear pore complex protein Nup98-Nup96</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">nup98</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">SDA103</strain>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="22">Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. NUP98 and NUP96 are involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC. In cooperation with DHX9, plays a role in transcription and alternative splicing activation of a subset of genes. Involved in the localization of DHX9 in discrete intranuclear foci (GLFG-body).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Nucleoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the nucleoporin GLFG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017348356.1">
        <property type="nucleotide sequence ID" value="XM_017492867.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000013263"/>
    <dbReference type="GeneID" id="108279002"/>
    <dbReference type="KEGG" id="ipu:108279002"/>
    <dbReference type="CTD" id="4928"/>
    <dbReference type="OMA" id="PMGKGLN"/>
    <dbReference type="OrthoDB" id="3797628at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044614">
        <property type="term" value="C:nuclear pore cytoplasmic filaments"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048484">
        <property type="term" value="P:enteric nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:RNA export from nucleus"/>
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        <property type="term" value="P:telomere tethering at nuclear periphery"/>
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        <property type="entry name" value="Nuclear pore complex protein Nup98-Nup96"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Peptidase S59, nucleoporin"/>
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        <property type="entry name" value="Nucleoporin_S59-like"/>
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        <property type="entry name" value="Nup98_C"/>
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    <dbReference type="PANTHER" id="PTHR23198:SF6">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP98-NUP96"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NUCLEOPORIN"/>
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        <property type="entry name" value="Nucleoporin2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Nup96"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nup98_GLEBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82215">
        <property type="entry name" value="C-terminal autoproteolytic domain of nucleoporin nup98"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NUP_C"/>
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    </dbReference>
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    <keyword evidence="17" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="19" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-0653">Protein transport</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0720">Serine protease</keyword>
    <keyword evidence="18" id="KW-0811">Translocation</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Peptidase S59" evidence="24">
        <location>
            <begin position="727"/>
            <end position="869"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="507"/>
            <end position="577"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="603"/>
            <end position="627"/>
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    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="686"/>
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            <begin position="520"/>
            <end position="529"/>
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            <begin position="703"/>
            <end position="712"/>
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            <dbReference type="ARBA" id="ARBA00004567"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004620"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008926"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013472"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
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            <dbReference type="ARBA" id="ARBA00022670"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
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            <dbReference type="ARBA" id="ARBA00023132"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00059309"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS51434"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_017348356.1"/>
        </source>
    </evidence>
    <sequence length="1806" mass="195249" checksum="680540AFCD2DB3D3" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T0Z9</accession>
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        <submittedName>
            <fullName evidence="9">Solute carrier family 13 member 2 isoform X1</fullName>
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        <name evidence="9" type="primary">slc13a2</name>
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        <name type="scientific">Ictalurus punctatus</name>
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        <name type="synonym">Silurus punctatus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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            <taxon>Ostariophysi</taxon>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">SDA103</strain>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
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    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    <comment type="similarity">
        <text evidence="2">Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily.</text>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:sodium:dicarboxylate symporter activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:succinate transmembrane transporter activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071285">
        <property type="term" value="P:cellular response to lithium ion"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01115">
        <property type="entry name" value="SLC13_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF82">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="6" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="282"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="347"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="384"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="450"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="487"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="532"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="572"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017348589.1"/>
        </source>
    </evidence>
    <sequence length="612" mass="68147" checksum="71DAF6968033B9DE" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T1S4</accession>
    <name>A0A2D0T1S4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Solute carrier family 3 member 2b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">slc3a2b</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5 6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017348740.1">
        <property type="nucleotide sequence ID" value="XM_017493251.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017348741.1">
        <property type="nucleotide sequence ID" value="XM_017493252.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T1S4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030972"/>
    <dbReference type="GeneID" id="108279203"/>
    <dbReference type="KEGG" id="ipu:108279203"/>
    <dbReference type="CTD" id="399488"/>
    <dbReference type="GeneTree" id="ENSGT00940000156646"/>
    <dbReference type="OMA" id="EPARYAH"/>
    <dbReference type="OrthoDB" id="1740265at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0015173">
        <property type="term" value="F:aromatic amino acid transmembrane transporter activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0015180">
        <property type="term" value="F:L-alanine transmembrane transporter activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015190">
        <property type="term" value="F:L-leucine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904273">
        <property type="term" value="P:L-alanine import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903801">
        <property type="term" value="P:L-leucine import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015823">
        <property type="term" value="P:phenylalanine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042280">
        <property type="entry name" value="SLC3A2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031984">
        <property type="entry name" value="SLC3A2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46673">
        <property type="entry name" value="4F2 CELL-SURFACE ANTIGEN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46673:SF3">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 3 (AMINO ACID TRANSPORTER HEAVY CHAIN), MEMBER 2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16028">
        <property type="entry name" value="SLC3A2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="3">
        <location>
            <begin position="123"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017348740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017348741.1"/>
        </source>
    </evidence>
    <sequence length="507" mass="55938" checksum="EA9917C470921EE1" modified="2017-12-20" version="1">MSKDAEVDMKDVELNDVDQERQPMTAGNGDANSALAVKNGIVKVKIPDEKESKFTGLSKEELLQVAGTPAWVRARWVLLILFWLGWLGMLAGAVVIIVQAPRCKPLPEINWWDSGPLYQIGDVDAFAGSSGLKGLETKVGALGQLKVKGLIVGPIHVSTADQSEDLNLIELTPSAGSLSDLEALIKAAHKKSIAVVLDLTPNYKGSEPWFLKSSVTNVTEKVKIATDYWLKKGIDGILLYGVERVSSVVPSFWPSIRETVDNHTKDEEKKVLIGATQATSPDEVNKLLNQTGVDLLLSDVLRNKSGAEIGQVVQSLYSQNGRRLAWNLGHRVGGHLANMVESVTIKLYQLLLLTLPGTPIFNYGDEIGLKDKSTTNPVMIWDSSNTNETEKSEVEQRNSLHMFFKAVSDLRGKERSLLHGDYVPLYKSTSTLAYLRTWDQSERYLVAFNFSPKDRASLQLSHEMLPEEAKVEVSTNKEEMAPGQFVNLAQLELAPQQAVMLKFPYIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T429</accession>
    <name>A0A2D0T429_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Zinc finger protein 385C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">znf385c</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017349659.1">
        <property type="nucleotide sequence ID" value="XM_017494170.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T429"/>
    <dbReference type="GeneID" id="108279727"/>
    <dbReference type="KEGG" id="ipu:108279727"/>
    <dbReference type="CTD" id="201181"/>
    <dbReference type="OrthoDB" id="434647at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000121">
        <property type="entry name" value="zinc finger protein 385B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051845">
        <property type="entry name" value="Znf385"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23067">
        <property type="entry name" value="DOUBLE-STRANDED RNA-BINDING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23067:SF6">
        <property type="entry name" value="ZINC FINGER PROTEIN 385C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="404"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="35"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="212"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="290"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="417"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="212"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="294"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="318"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017349659.1"/>
        </source>
    </evidence>
    <sequence length="558" mass="61331" checksum="E9E0CC22914DE62B" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T4E3</accession>
    <name>A0A2D0T4E3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">exodeoxyribonuclease III</fullName>
            <ecNumber evidence="4">3.1.11.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">APEX nuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Apurinic-apyrimidinic endonuclease 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">apex1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 18">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="16 18">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="16 18">Probably binds two magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.2" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017349773.1">
        <property type="nucleotide sequence ID" value="XM_017494284.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T4E3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008450"/>
    <dbReference type="GeneID" id="108279796"/>
    <dbReference type="KEGG" id="ipu:108279796"/>
    <dbReference type="CTD" id="328"/>
    <dbReference type="OMA" id="WWSYRGR"/>
    <dbReference type="OrthoDB" id="498125at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008311">
        <property type="term" value="F:double-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09087">
        <property type="entry name" value="Ape1-like_AP-endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000009">
        <property type="entry name" value="DNA-(apurinic or apyrimidinic site) lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020847">
        <property type="entry name" value="AP_endonuclease_F1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020848">
        <property type="entry name" value="AP_endonuclease_F1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00195">
        <property type="entry name" value="exoDNase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00633">
        <property type="entry name" value="xth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="AP ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748:SF6">
        <property type="entry name" value="DNA-(APURINIC OR APYRIMIDINIC SITE) ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00726">
        <property type="entry name" value="AP_NUCLEASE_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00728">
        <property type="entry name" value="AP_NUCLEASE_F1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 18" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 18" id="KW-0234">DNA repair</keyword>
    <keyword evidence="18" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 18 22" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 16 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 16 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 18" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12 18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="20">
        <location>
            <begin position="60"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="15">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="17">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="17">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA substrate" evidence="17">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR604808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017349773.1"/>
        </source>
    </evidence>
    <sequence length="311" mass="34981" checksum="6180FF7F20547ADD" modified="2017-12-20" version="1">MPKRAKKNEASAEAGNDNGTEPETKKGKKAKEPEAPVLYEDPPDKLTSKSGRAANMKLTSWNVDGLRAWVKKKGLDWVRQESPDVLCLQETKCAEKALPADITSMPEYPYKYWSGSEDKEGYSGVAMLCKTEPLDVTYGIGKEEHDKEGRVITAEFPSFFLVTAYVPNAGRGLVRLDYRKTWDVDFREYLSDLDSRKPLVLCGDLNVAHQEIDLKNPKGNRKNAGFTPEEREGFSQLLAAGFTDSFRELYPEQTNAYTFWTYMMNARGKNVGWRLDYFLLSAALLPSLCDSKIRNTAMGSDHCPITLHLAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T7N7</accession>
    <name>A0A2D0T7N7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Trimethylguanosine synthase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Cap-specific guanine-N(2) methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Nuclear receptor coactivator 6-interacting protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">PRIP-interacting protein with methyltransferase motif</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">tgs1</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">SDA103</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78479"/>
            <dbReference type="Rhea" id="RHEA-COMP:19087"/>
            <dbReference type="Rhea" id="RHEA-COMP:19089"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:167623"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:78480"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(2),N(7)-trimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78507"/>
            <dbReference type="Rhea" id="RHEA-COMP:19088"/>
            <dbReference type="Rhea" id="RHEA-COMP:19090"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:167623"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:78508"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78471"/>
            <dbReference type="Rhea" id="RHEA-COMP:19085"/>
            <dbReference type="Rhea" id="RHEA-COMP:19087"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:78472"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-methionine = a 5'-end (N(2),N(7)-dimethyl 5'-triphosphoguanosine)-ribonucleoside in snoRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78475"/>
            <dbReference type="Rhea" id="RHEA-COMP:19086"/>
            <dbReference type="Rhea" id="RHEA-COMP:19088"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:172880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:78476"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="20">May form homooligomers. Interacts with CREBBP/CBP, EED/WAIT1, EP300/P300, NCOA6/PRIP, PPARBP/PBP and SMN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Cajal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the methyltransferase superfamily. Trimethylguanosine synthase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017350894.1">
        <property type="nucleotide sequence ID" value="XM_017495405.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T7N7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003249"/>
    <dbReference type="GeneID" id="108280441"/>
    <dbReference type="KEGG" id="ipu:108280441"/>
    <dbReference type="CTD" id="96764"/>
    <dbReference type="OMA" id="QSEPHNG"/>
    <dbReference type="OrthoDB" id="194443at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071164">
        <property type="term" value="F:RNA cap trimethylguanosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000066">
        <property type="entry name" value="Trimethylguanosine synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019012">
        <property type="entry name" value="RNA_cap_Gua-N2-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741">
        <property type="entry name" value="S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14741:SF32">
        <property type="entry name" value="TRIMETHYLGUANOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09445">
        <property type="entry name" value="Methyltransf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="108"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="273"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="338"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="507"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="566"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="151"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="168"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="276"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="381"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="573"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="23">
        <location>
            <begin position="616"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00057179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00064494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00079339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00081504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_017350894.1"/>
        </source>
    </evidence>
    <sequence length="854" mass="97485" checksum="C028CFC3C90F7E08" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EXD4</accession>
    <name>A0A979EXD4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein LOC108268227 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC108268227</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047012482.1">
        <property type="nucleotide sequence ID" value="XM_047156526.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EXD4"/>
    <dbReference type="GeneID" id="108268227"/>
    <dbReference type="KEGG" id="ipu:108268227"/>
    <dbReference type="OMA" id="ENCHEYN"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32046">
        <property type="entry name" value="G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32046:SF11">
        <property type="entry name" value="IMMUNE-ASSOCIATED NUCLEOTIDE-BINDING PROTEIN 10-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_047012482.1"/>
        </source>
    </evidence>
    <sequence length="571" mass="65915" checksum="ACB853C0EA19E996" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EZW7</accession>
    <name>A0A979EZW7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ankyrin repeat and EF-hand domain-containing protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ankef1b</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047013458.1">
        <property type="nucleotide sequence ID" value="XM_047157502.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EZW7"/>
    <dbReference type="GeneID" id="108269963"/>
    <dbReference type="CTD" id="100149106"/>
    <dbReference type="OMA" id="WSCEHET"/>
    <dbReference type="OrthoDB" id="539213at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052801">
        <property type="entry name" value="Ankyrin-EF-hand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24127">
        <property type="entry name" value="ANKYRIN REPEAT AND EF-HAND DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24127:SF1">
        <property type="entry name" value="ANKYRIN REPEAT AND EF-HAND DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="110"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="143"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="247"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="313"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="436"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="594"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="627"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="660"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="479"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="724"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="481"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="743"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_047013458.1"/>
        </source>
    </evidence>
    <sequence length="854" mass="94681" checksum="4518A7A66BF035D5" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QU58</accession>
    <name>A0A9F7QU58_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nuclear transcription factor Y subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 9 10" type="primary">LOC128628670</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8 9 10" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8 9 10">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NFYA/HAP2 subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053529945.1">
        <property type="nucleotide sequence ID" value="XM_053673970.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053529946.1">
        <property type="nucleotide sequence ID" value="XM_053673971.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053529947.1">
        <property type="nucleotide sequence ID" value="XM_053673972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QU58"/>
    <dbReference type="GeneID" id="128628670"/>
    <dbReference type="KEGG" id="ipu:128628670"/>
    <dbReference type="OrthoDB" id="1097733at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001289">
        <property type="entry name" value="NFYA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12632">
        <property type="entry name" value="TRANSCRIPTION FACTOR NF-Y ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02045">
        <property type="entry name" value="CBFB_NFYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00616">
        <property type="entry name" value="CCAATSUBUNTB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00521">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51152">
        <property type="entry name" value="NFYA_HAP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053529945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_053529946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053529947.1"/>
        </source>
    </evidence>
    <sequence length="344" mass="36512" checksum="987ED39589536545" modified="2023-06-28" version="1">MEQFAESTSGGEQIVVQPSSGAVQQQSAVTAVQLQADGQVDVGSGQQVQTLQVVEGQPLMVQVSGGQLITPSGQPIMVQAMPGGQAQTIMQLPVTGSQGLQQIQLVQPGQIQLSGGQTLQVQTAQGQTQQIIIQQLQTAVTAGQNQPQQITVQGEQAQTAEGQTILYLPVNADGTILQQGVITIPAASLAGGQIIQAGSNVSGTGGQGTVTVAVPVTGNMVNAGGMVMMVQSGGSVPTMQRIPLPGAEMLEEEPLYVNPKQYHRILKRRQARAKLEAEGKIPKRRRKYLHESRHRHAMARKRGDGGRFFSGKEKDELDVQVNVKPRVGIVTARTSLLKDEETKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QXC2</accession>
    <name>A0A9F7QXC2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP-dependent RNA helicase TDRD9-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC128630157</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053534755.1">
        <property type="nucleotide sequence ID" value="XM_053678780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QXC2"/>
    <dbReference type="GeneID" id="128630157"/>
    <dbReference type="KEGG" id="ipu:128630157"/>
    <dbReference type="OrthoDB" id="66977at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_053534755.1"/>
        </source>
    </evidence>
    <sequence length="128" mass="14361" checksum="439A5349568C9751" modified="2023-06-28" version="1">MHISETSPSDYTSTHTQKFIVQVAIAGAFYPNCFLQGTVDEELASKELYGNDPRTTVVVCNLPLFAFLYYKQLQSLFRHCGQVKSISFDSSRAYVEFYCSSMRGSGVLPEVSLSLLLAQQRLPLHLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QXI0</accession>
    <name>A0A9F7QXI0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC128628997</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">SDA103</strain>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="3">Blood</tissue>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_053530841.1"/>
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                <person name="Liu S."/>
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                <person name="Bao L."/>
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                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
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                <person name="Geng X."/>
                <person name="Song L."/>
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                <person name="Wang X."/>
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                <person name="Yuan Z."/>
                <person name="Yang Y."/>
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                <person name="Lu J."/>
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                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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            <tissue evidence="3">Blood</tissue>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
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    <sequence length="147" mass="16330" checksum="A09A7B913DE0366B" modified="2023-06-28" version="1">MKNLTKKDSGRYQLRFLTNDGNRFSAEPRVILNVTDLQVRASRSAVASEGQTTVTLSCITSCTLSNNPTYMWYKNGQPVTDKLTKHNKLYLISSEDAGNYSCAVTGHEDLRSPELTVTCEPQNSVIILMYRVVIAQVQSSALTESSF</sequence>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Liu S."/>
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                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
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                <person name="Wang X."/>
                <person name="Chen A."/>
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                <person name="Yuan Z."/>
                <person name="Yang Y."/>
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                <person name="Duke M.V."/>
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                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="7">Blood</tissue>
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    <dbReference type="KEGG" id="ipu:108262888"/>
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        <source>
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            <dbReference type="PROSITE" id="PS50102"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
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            <dbReference type="RefSeq" id="XP_053535056.1"/>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Li Y."/>
                <person name="Jiang C."/>
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                <person name="Zhang Y."/>
                <person name="Zhou T."/>
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                <person name="Li N."/>
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                <person name="Phillippy A.M."/>
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                <person name="Song L."/>
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                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
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                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
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                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
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    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
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        <property type="nucleotide sequence ID" value="XM_053674183.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A9F7R4W4"/>
    <dbReference type="GeneID" id="108281061"/>
    <dbReference type="KEGG" id="ipu:108281061"/>
    <dbReference type="CTD" id="799435"/>
    <dbReference type="OrthoDB" id="28697at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051496">
        <property type="term" value="P:positive regulation of stress fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032933">
        <property type="term" value="P:SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000200">
        <property type="entry name" value="Rho guanine nucleotide exchange factor (GEF) 10-like a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000003">
        <property type="entry name" value="Rho guanine nucleotide exchange factor 10 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039919">
        <property type="entry name" value="ARHGEF10/ARHGEF17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
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    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12877">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12877:SF16">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 10-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19057">
        <property type="entry name" value="PH_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19056">
        <property type="entry name" value="WD40_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="5">
        <location>
            <begin position="308"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="127"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1113"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="478"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="20"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1123"/>
            <end position="1136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053530158.1"/>
        </source>
    </evidence>
    <sequence length="1254" mass="138327" checksum="EC308B1839C7A763" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RCM1</accession>
    <name>A0A9F7RCM1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Macrophage mannose receptor 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC128628782</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053537652.1">
        <property type="nucleotide sequence ID" value="XM_053681677.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="128628782"/>
    <dbReference type="KEGG" id="ipu:128628782"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
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        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 20 MEMBER A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784:SF3">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 4 MEMBER K-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039933203" evidence="2">
        <location>
            <begin position="22"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="21"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="136"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053537652.1"/>
        </source>
    </evidence>
    <sequence length="263" mass="30571" checksum="23CA2E920E978743" modified="2023-06-28" version="1">MGSAVNFILLLSAGLWAPALSASRQFYAVEENRDWSEAQTWCRENYTDLATIENQEEMDAVKDLLKGKGGNFWIGLKQNKTYWHIYKNRSWYWSDGAPYNYTYWQNGEPNNEEGDNCVEVYSGNNTWNDAGCHHRHSFVCYKKRTPLTVITEKKTWREALRYCRQNHVDLVSVDSQEMQDWVEVVTKNVYSDMWIGLRHTCALGFWYSVKGEMICYQNWAPGNGTGVEDCGDVERTGAVLSESKKWVSLPQTHELYFICITFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RGS2</accession>
    <name>A0A9F7RGS2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Kinetochore-associated protein 1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC128630863</name>
        <name evidence="3" type="synonym">LOC128630035</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3 4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053534594.1">
        <property type="nucleotide sequence ID" value="XM_053678619.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053535152.1">
        <property type="nucleotide sequence ID" value="XM_053679177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RGS2"/>
    <dbReference type="GeneID" id="128630863"/>
    <dbReference type="KEGG" id="ipu:128630035"/>
    <dbReference type="KEGG" id="ipu:128630863"/>
    <dbReference type="OrthoDB" id="8972924at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005828">
        <property type="term" value="C:kinetochore microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990423">
        <property type="term" value="C:RZZ complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903394">
        <property type="term" value="P:protein localization to kinetochore involved in kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052802">
        <property type="entry name" value="KNTC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019527">
        <property type="entry name" value="RZZ-complex_KNTC1/ROD_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15688">
        <property type="entry name" value="KINETOCHORE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15688:SF1">
        <property type="entry name" value="KINETOCHORE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10493">
        <property type="entry name" value="Rod_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RZZ complex subunit KNTC1/ROD C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053534594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053535152.1"/>
        </source>
    </evidence>
    <sequence length="76" mass="8554" checksum="A2A8757AC9CFA8B8" modified="2023-06-28" version="1">MHAVAKEIVPINGADLLNISNILLEKWLCTPGQTNGKDLQYQDSVTDLTEDPDLMRVVYLLQMHSMDMRHVCSVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RNL1</accession>
    <name>A0A9F7RNL1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DET1- and DDB1-associated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">dda1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. In the DCX complexes, acts as a scaffolding subunit required to stabilize the complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DDA1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053539007.1">
        <property type="nucleotide sequence ID" value="XM_053683032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RNL1"/>
    <dbReference type="GeneID" id="100529024"/>
    <dbReference type="CTD" id="79016"/>
    <dbReference type="OrthoDB" id="8598182at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033575">
        <property type="entry name" value="DDA1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018276">
        <property type="entry name" value="DDA1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31879">
        <property type="entry name" value="DET1- AND DDB1-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31879:SF2">
        <property type="entry name" value="DET1- AND DDB1-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10172">
        <property type="entry name" value="DDA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DET1- and DDB1-associated protein 1" evidence="5">
        <location>
            <begin position="5"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="90"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00045586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053539007.1"/>
        </source>
    </evidence>
    <sequence length="123" mass="14265" checksum="238E43A82A7530B0" modified="2023-06-28" version="1">MDKADFLKGLPVYNKSNFSRFHADSVCKASNRRPSVYLPTREYPSEQIIVTEKTNILLRYLHQQWDKKKSRFIRPGYVFPVFNCPVLNAAKKREREQEQGEGGSPAPSRKIARTDSQEMNEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TB12</accession>
    <name>A0A9F7TB12_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Guanine nucleotide-binding protein G(i) subunit alpha-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Adenylate cyclase-inhibiting G alpha protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">gnai2a</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-alpha family. G(i/o/t/z) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053529889.1">
        <property type="nucleotide sequence ID" value="XM_053673914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TB12"/>
    <dbReference type="GeneID" id="108254730"/>
    <dbReference type="CTD" id="336421"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007193">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001973">
        <property type="term" value="P:G protein-coupled adenosine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide-binding protein G(I) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002487">
        <property type="entry name" value="Guanine nucleotide-binding protein G(i) subunit alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001408">
        <property type="entry name" value="Gprotein_alpha_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF73">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN G(I) SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00441">
        <property type="entry name" value="GPROTEINAI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11 21" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10 22" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0519">Myristate</keyword>
    <keyword evidence="9 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0564">Palmitate</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="99"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="124"/>
            <end position="130"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="149"/>
            <end position="153"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="218"/>
            <end position="221"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00037181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00039891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00042569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_053529889.1"/>
        </source>
    </evidence>
    <sequence length="303" mass="34836" checksum="6222DDBF74F4B0DF" modified="2023-06-28" version="1">MKIIHEDGYSEDECKQYRAVVYSNTIQSIMAIIKAMSNLKIDYEEPARADDARQLFALAAGAEEQGLFPDDLSGIVSRLWADGGVQNCFARSREYQLNDSAAYYLNDLERIAKADYIPTQSDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVAMSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTETSIILFLNKKDLFETKIEHSPLTICFPDYTGSSTYAEASEFIRTKFEDLNKKKDTKEIYSHFTCATDTENIQFVFDAVTDVIIKNNLRDCGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TCI6</accession>
    <name>A0A9F7TCI6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Homologous recombination OB-fold protein-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC108276643</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053531914.1">
        <property type="nucleotide sequence ID" value="XM_053675939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TCI6"/>
    <dbReference type="GeneID" id="108276643"/>
    <dbReference type="KEGG" id="ipu:108276643"/>
    <dbReference type="OrthoDB" id="21443at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028045">
        <property type="entry name" value="HROB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058570">
        <property type="entry name" value="HROB_OB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14523:SF1">
        <property type="entry name" value="HOMOLOGOUS RECOMBINATION OB-FOLD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14523">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN C17ORF53 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15072">
        <property type="entry name" value="HROB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Homologous recombination OB-fold protein OB-fold" evidence="3">
        <location>
            <begin position="191"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053531914.1"/>
        </source>
    </evidence>
    <sequence length="365" mass="40682" checksum="73374362EEA44F9B" modified="2023-06-28" version="1">MTFLSALLAAVVYFLLHPLLYMVIAAATFYYYVKRRSREKTTAGPEDTTEDSLFDQGRELAVVQPTEVPQDQVTELVKILNRILSDDRMKELEECICKALRNWKIKSKISGWGLDDIFVAVTQALAHGAVACTRSEVPSSQVNDEEEFSVGPWAAMKAEMGLDERNPSCFLHLYSVVMVLRKAASRQVSMNKVPNMAVVLKSILHTQGDAIAVFRDPTGEMQGTVHRRLLEERQGELKTGAVLLLKQVSVFSTSNQNHYLNVTPNNLLRIYPPDGAVHASSQHSQPALPEGGPISQMELHFDDHEDEEQEQGSIPAKPRVPANGVVQSTTRPDCSEDAQWDADDPDELLLALRRVLLRLEPTPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TD15</accession>
    <name>A0A9F7TD15_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Proheparin-binding EGF-like growth factor isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">areg</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053530433.1">
        <property type="nucleotide sequence ID" value="XM_053674458.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TD15"/>
    <dbReference type="GeneID" id="108255648"/>
    <dbReference type="KEGG" id="ipu:108255648"/>
    <dbReference type="CTD" id="374"/>
    <dbReference type="OrthoDB" id="9909110at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005154">
        <property type="term" value="F:epidermal growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007173">
        <property type="term" value="P:epidermal growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045840">
        <property type="term" value="P:positive regulation of mitotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10740:SF16">
        <property type="entry name" value="AMPHIREGULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10740">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039920275" evidence="6">
        <location>
            <begin position="28"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="179"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="120"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="38"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="84"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_053530433.1"/>
        </source>
    </evidence>
    <sequence length="232" mass="24852" checksum="EFA5F6EC26E8273D" modified="2023-06-28" version="1">MNLILLSSLLCLACSVLISLASPATHASEMSHMTVMSASGEGLQSALGEEPESEDGPSGLFTGPPLGKGRGGRKKHGGKKRNKAQVSVRQRNSTPAQNLSYSPSHSTSRSSSHSTNHSSAPDPCSTSHREYCIHGYCTYLQDLKEPICVCMKGYDGMRCGIQLLQTGSMGRDDHHDDTLHITLITITVVLSIISCSALLLIIYVHYRAQHSFQAALLSAADEGDKLQNNSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TRR0</accession>
    <name>A0A9F7TRR0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein LOC108276731 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC108276731</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
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    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
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    <dbReference type="AlphaFoldDB" id="A0A9F7TRR0"/>
    <dbReference type="GeneID" id="108276731"/>
    <dbReference type="KEGG" id="ipu:108276731"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039949339" evidence="2">
        <location>
            <begin position="22"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053543001.1"/>
        </source>
    </evidence>
    <sequence length="147" mass="16100" checksum="4C21B7C745F1C4CE" modified="2023-06-28" version="1">MRAAVPVLLLVALAGCRSVFCVSLDVAAKEDGSDERVEISDLQKSDETKSEAESEEERMAHDNCFGKAVRDQPEQKFNPTVSSVSEEITRGSETQNTTETKTEDEGTEARAEVQALSLDMAEEDSDEERSDIKLLKRLAGDTSVAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2009-09-01" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>C6KE06</accession>
    <name>C6KE06_ICTPU</name>
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        <submittedName>
            <fullName evidence="17 20">CD8 alpha</fullName>
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    </protein>
    <gene>
        <name evidence="20" type="primary">cd8a</name>
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    <organism evidence="17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
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            <taxon>Ictaluridae</taxon>
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    <reference evidence="17" key="1">
        <citation type="submission" date="2009-05" db="EMBL/GenBank/DDBJ databases">
            <title>Channel catfish, Ictalurus punctatus, CD8 molecules.</title>
            <authorList>
                <person name="Quiniou S.M."/>
                <person name="Sahoo M."/>
                <person name="Bengten E."/>
                <person name="Wilson M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2010-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Quiniou S."/>
                <person name="Sahoo M."/>
                <person name="Edholm E."/>
                <person name="Bengten E."/>
                <person name="Wilson M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="18 20" key="3">
        <citation type="journal article" date="2011" name="Fish Shellfish Immunol." volume="30" first="894" last="901">
            <title>Channel catfish CD8alpha and CD8beta co-receptors: characterization, expression and polymorphism.</title>
            <authorList>
                <person name="Quiniou S.M."/>
                <person name="Sahoo M."/>
                <person name="Edholm E.S."/>
                <person name="Bengten E."/>
                <person name="Wilson M."/>
            </authorList>
            <dbReference type="PubMed" id="21272650"/>
            <dbReference type="DOI" id="10.1016/j.fsi.2011.01.011"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="20" key="4">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="19" key="5">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="6">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="GQ179649">
        <property type="protein sequence ID" value="ACS66820.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="HQ446239">
        <property type="protein sequence ID" value="AED99295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187260.1">
        <property type="nucleotide sequence ID" value="NM_001200331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="C6KE06"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000017467"/>
    <dbReference type="GeneID" id="100415908"/>
    <dbReference type="KEGG" id="ipu:100415908"/>
    <dbReference type="CTD" id="925"/>
    <dbReference type="OrthoDB" id="9906515at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015468">
        <property type="entry name" value="CD8_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10441">
        <property type="entry name" value="CD8 ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10441:SF2">
        <property type="entry name" value="T-CELL SURFACE GLYCOPROTEIN CD8 ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 16 20" id="KW-0732">Signal</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5011323924" evidence="16 20">
        <location>
            <begin position="21"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="203"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ACS66820.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AED99295.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="NP_001187260.1"/>
        </source>
    </evidence>
    <sequence length="223" mass="24818" checksum="6E480A9D0F4C50F8" modified="2009-09-01" version="1">MALILSVLCVVLLGFHGVEGNVKELVIKEKQPATITCDKNYPSTVFWLRLKENGQGFEYIASYSKTKKSGKVAEGGNYKVAEKTFDINSFETQKDSGTYSCVFINNNQLEFSSITKLRGETVPTTIKPKVQTTPMPTVPTTKAVVTCGQNSRGKKAEKIDVLLGCELHIFIPLAAGCGFLLLLLLITILYCNRIRTRRCPHHYKRQPRGRAPGHKTLPNPPDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3TED7</accession>
    <name>E3TED7_ICTPU</name>
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    <gene>
        <name evidence="5" type="primary">GGCT</name>
        <name evidence="7" type="synonym">ggct</name>
        <name evidence="7" type="synonym">ggctb</name>
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    <organism evidence="5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="5 7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
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                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
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                <person name="Abernathy J."/>
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                <person name="Liu S."/>
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                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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                <person name="Zeng Q."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Zhang Y."/>
                <person name="Yuan Z."/>
                <person name="Jiang C."/>
                <person name="Chen A."/>
                <person name="Fu Q."/>
                <person name="Su B."/>
                <person name="Dunham R."/>
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    <reference evidence="8" key="4">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
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    </evidence>
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        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
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                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
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                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
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            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Tom5 family.</text>
    </comment>
    <dbReference type="EMBL" id="GU588832">
        <property type="protein sequence ID" value="ADO28786.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001188147.1">
        <property type="nucleotide sequence ID" value="NM_001201218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TEQ0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000004673"/>
    <dbReference type="GeneID" id="100528948"/>
    <dbReference type="KEGG" id="ipu:100528948"/>
    <dbReference type="CTD" id="401505"/>
    <dbReference type="GeneTree" id="ENSGT00940000163364"/>
    <dbReference type="OMA" id="QIYCSSE"/>
    <dbReference type="OrthoDB" id="8893106at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005742">
        <property type="term" value="C:mitochondrial outer membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006626">
        <property type="term" value="P:protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019603">
        <property type="entry name" value="Tom5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029179">
        <property type="entry name" value="TOMM5_metazoa"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28436">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28436:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10642">
        <property type="entry name" value="Tom5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADO28786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="NP_001188147.1"/>
        </source>
    </evidence>
    <sequence length="51" mass="5981" checksum="DF356FA890CACEFE" modified="2011-01-11" version="1">MFKLEGLGPKLDPEEMKRKMREDVISSVRNFLIYVALLRVTPYVLKKLDSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3TFQ8</accession>
    <name>E3TFQ8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SH3 domain-binding glutamic acid-rich-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SH3L2</name>
        <name evidence="8" type="synonym">sh3bgrl2</name>
        <name evidence="8" type="synonym">sh3l2</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the SH3BGR family.</text>
    </comment>
    <dbReference type="EMBL" id="GU589192">
        <property type="protein sequence ID" value="ADO29144.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001188088.1">
        <property type="nucleotide sequence ID" value="NM_001201159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TFQ8"/>
    <dbReference type="GeneID" id="100528889"/>
    <dbReference type="KEGG" id="ipu:100528889"/>
    <dbReference type="CTD" id="83699"/>
    <dbReference type="GeneTree" id="ENSGT00940000159157"/>
    <dbReference type="OMA" id="MYKNIPK"/>
    <dbReference type="OrthoDB" id="9932926at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03030">
        <property type="entry name" value="GRX_SH3BGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006993">
        <property type="entry name" value="Glut_rich_SH3-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051033">
        <property type="entry name" value="SH3BGR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12232">
        <property type="entry name" value="SH3 DOMAIN-BINDING GLUTAMIC ACID-RICH-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12232:SF4">
        <property type="entry name" value="SH3 DOMAIN-BINDING GLUTAMIC ACID-RICH-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04908">
        <property type="entry name" value="SH3BGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008142">
        <property type="entry name" value="SH3-bind_E-rich_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0729">SH3-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADO29144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="NP_001188088.1"/>
        </source>
    </evidence>
    <sequence length="111" mass="12624" checksum="619DBD6406429C6E" modified="2011-01-11" version="1">MVIRVYVASSSGSLALKKHQQEVVGFLEANRIHFEEVDITMLEDQRFWMYKNIPEEKRPEKGNPLPPQIFNGTVYCGDYEDFFLSKEANTVFSFLGLNSQASGKDVESDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-07-05" modified="2026-01-28" version="100" xmlns="https://uniprot.org/uniprot">
    <accession>Q6QLZ5</accession>
    <name>Q6QLZ5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Myogenic factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">myod1</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2004" name="Anim. Genet." volume="35" first="425" last="430">
            <title>Cloning and characterization of myogenic regulatory genes in three Ictalurid species.</title>
            <authorList>
                <person name="Gregory D.J."/>
                <person name="Waldbieser G.C."/>
                <person name="Bosworth B.G."/>
            </authorList>
            <dbReference type="PubMed" id="15566463"/>
            <dbReference type="DOI" id="10.1111/j.1365-2052.2004.01193.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2 11">Efficient DNA binding requires dimerization with another bHLH protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AY534328">
        <property type="protein sequence ID" value="AAS48083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017341011.1">
        <property type="nucleotide sequence ID" value="XM_017485522.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q6QLZ5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006751"/>
    <dbReference type="GeneID" id="108275001"/>
    <dbReference type="KEGG" id="ipu:108275001"/>
    <dbReference type="CTD" id="4654"/>
    <dbReference type="GeneTree" id="ENSGT00950000182959"/>
    <dbReference type="OMA" id="GPMEMTE"/>
    <dbReference type="OrthoDB" id="10049614at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043282">
        <property type="term" value="P:chordate pharyngeal muscle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002074">
        <property type="term" value="P:extraocular skeletal muscle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045663">
        <property type="term" value="P:positive regulation of myoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048743">
        <property type="term" value="P:positive regulation of skeletal muscle fiber development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035914">
        <property type="term" value="P:skeletal muscle cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048741">
        <property type="term" value="P:skeletal muscle fiber development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd18936">
        <property type="entry name" value="bHLH_TS_MYOD1_Myf3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000005">
        <property type="entry name" value="Myogenic factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022032">
        <property type="entry name" value="Myf5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002546">
        <property type="entry name" value="MyoD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039704">
        <property type="entry name" value="Myogenic_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11534:SF2">
        <property type="entry name" value="MYOBLAST DETERMINATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11534">
        <property type="entry name" value="MYOGENIC FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01586">
        <property type="entry name" value="Basic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12232">
        <property type="entry name" value="Myf5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00520">
        <property type="entry name" value="BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0517">Myogenesis</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="13">
        <location>
            <begin position="84"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="152"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="223"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="154"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="223"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="243"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AAS48083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017341011.1"/>
        </source>
    </evidence>
    <sequence length="269" mass="29971" checksum="02A45B4F7F8668E1" modified="2004-07-05" version="1">MELSDIPFPIPSADDFYDDPCFNTSDMHFFEDLDPRLVHVSLLKSDEHNHMEDEHIRAPSGHHQAGRCLLWACKACKRKTTNADRRKAATMRERRRLSKVNDAFETLKRCTSTNPNQRLPKVEILRNAISYIESLQALLRSQEENYYPVLEQYSGDSDASSPRSNCSDGMMDFNGPARPSGRRNSYDTTYFNETPNGDVRSNKNPVVSSLDCLSSIVERISTESPAASSLPAAELCDGVTGSPPETSVPSPNNTCSPSGPTHDPIYQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5U5R9</accession>
    <name>W5U5R9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytohesin-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">PH, SEC7 and coiled-coil domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">CYTH2</name>
        <name evidence="18" type="synonym">cyth2</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT405358">
        <property type="protein sequence ID" value="AHH37160.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017328441.1">
        <property type="nucleotide sequence ID" value="XM_017472952.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U5R9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000191"/>
    <dbReference type="GeneID" id="108268159"/>
    <dbReference type="KEGG" id="ipu:108268159"/>
    <dbReference type="CTD" id="9266"/>
    <dbReference type="OrthoDB" id="430364at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01252">
        <property type="entry name" value="PH_GRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000002">
        <property type="entry name" value="Cytohesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.20:FF:000003">
        <property type="entry name" value="Cytohesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000009">
        <property type="entry name" value="Cytohesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF343">
        <property type="entry name" value="CYTOHESIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="15">
        <location>
            <begin position="71"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="14">
        <location>
            <begin position="276"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="35"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AHH37160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017328441.1"/>
        </source>
    </evidence>
    <sequence length="416" mass="48363" checksum="8FF537CDF88265F8" modified="2014-04-16" version="1">MTVGSDTYMPKSKAPKMDDLDYIPADLSPEERAELEEIRRRKGALLLEIQRLREELREAMMEVEGLENSTEGSKTLQKSRHVAMGRKKFNMDPKKGIAFLVENELLRHTPEEIAQFLYKGEGLNKTAIGDYLGERDEFNLRVLQAFVDLHEFTDLNLVQALRQFLWSFRLPGEAQKIDRMMEAFAQRYCHCNPGVFQSTDTCYVLSFAIIMLNTSLHNPNVRDKPTVERFISMNRGINEGGDLPEELLRNLYDSIKNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGRVKTWKRRWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVEDPRKPNCFELYIPNNRGQLIKACKTEADGRVVEGNHMVYRISAPTPEEKDEWIHSIKSAVSVDPFYEMLAARKKRISLKKKEEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5U5S1</accession>
    <name>W5U5S1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">receptor protein serine/threonine kinase</fullName>
            <ecNumber evidence="5">2.7.11.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">ACVR1</name>
        <name evidence="30 31" type="synonym">acvrl1</name>
    </gene>
    <organism evidence="28">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="28">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="29" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="29">SDA103</strain>
        </source>
    </reference>
    <reference evidence="30 31" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="30 31">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[receptor-protein] + ATP = O-phospho-L-seryl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18673"/>
            <dbReference type="Rhea" id="RHEA-COMP:11022"/>
            <dbReference type="Rhea" id="RHEA-COMP:11023"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-threonyl-[receptor-protein] + ATP = O-phospho-L-threonyl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44880"/>
            <dbReference type="Rhea" id="RHEA-COMP:11024"/>
            <dbReference type="Rhea" id="RHEA-COMP:11025"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.30" evidence="5"/>
    <dbReference type="EMBL" id="JT405762">
        <property type="protein sequence ID" value="AHH37311.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017342381.1">
        <property type="nucleotide sequence ID" value="XM_017486892.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047016342.1">
        <property type="nucleotide sequence ID" value="XM_047160386.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U5S1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012319"/>
    <dbReference type="GeneID" id="108275854"/>
    <dbReference type="KEGG" id="ipu:108275854"/>
    <dbReference type="CTD" id="94"/>
    <dbReference type="GeneTree" id="ENSGT00940000166145"/>
    <dbReference type="OrthoDB" id="69842at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070724">
        <property type="term" value="C:BMP receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004675">
        <property type="term" value="F:transmembrane receptor protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23534">
        <property type="entry name" value="TFP_LU_ECD_ALK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000018">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000064">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003605">
        <property type="entry name" value="GS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000333">
        <property type="entry name" value="TGFB_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255:SF66">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE RECEPTOR R3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR TYPE I AND II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08515">
        <property type="entry name" value="TGF_beta_GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00467">
        <property type="entry name" value="GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51256">
        <property type="entry name" value="GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="13 22 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 30 31" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="19" id="KW-0464">Manganese</keyword>
    <keyword evidence="16 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 22 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17 28 30" id="KW-0675">Receptor</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 23" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 25" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 24" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="25">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540049" description="receptor protein serine/threonine kinase" evidence="25">
        <location>
            <begin position="24"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="107"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="GS" evidence="27">
        <location>
            <begin position="153"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="26">
        <location>
            <begin position="183"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="EMBL" id="AHH37311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="RefSeq" id="XP_017342381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="RefSeq" id="XP_047016342.1"/>
        </source>
    </evidence>
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        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
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                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
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                <person name="Peatman E."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="17" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
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                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="17">SDA103</strain>
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        <citation type="submission" date="2025-04" db="UniProtKB">
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
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        <text evidence="10">Belongs to the ARTD/PARP family.</text>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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            <dbReference type="EMBL" id="AHH37598.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
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    </evidence>
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<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
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    <reference evidence="20" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
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                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
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    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, downstream of PINK1 and mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Binds to the intracellular domain of tumor necrosis factor type 1 receptor. Binds to RB1. Interacts with SRC. Interacts with SDHA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT406894">
        <property type="protein sequence ID" value="AHH37770.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017308199.1">
        <property type="nucleotide sequence ID" value="XM_017452710.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U7M8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007645"/>
    <dbReference type="GeneID" id="108256137"/>
    <dbReference type="KEGG" id="ipu:108256137"/>
    <dbReference type="CTD" id="10131"/>
    <dbReference type="GeneTree" id="ENSGT01020000230401"/>
    <dbReference type="OrthoDB" id="28737at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16927">
        <property type="entry name" value="HATPase_Hsp90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.790:FF:000004">
        <property type="entry name" value="Heat shock protein 75 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.80:FF:000004">
        <property type="entry name" value="Heat shock protein 75 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000021">
        <property type="entry name" value="Heat shock protein 75 kDa, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11260:FF:000004">
        <property type="entry name" value="Heat shock protein 75 mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.790">
        <property type="entry name" value="Heat shock protein 90, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00505">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037196">
        <property type="entry name" value="HSP90_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003555">
        <property type="entry name" value="PRK05218.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00183">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002583">
        <property type="entry name" value="Hsp90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00775">
        <property type="entry name" value="HEATSHOCK90"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110942">
        <property type="entry name" value="HSP90 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20 22" id="KW-0346">Stress response</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="19">
        <location>
            <begin position="115"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="185"/>
            <end position="186"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="207"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00066161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00073018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00076190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR002583-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="AHH37770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017308199.1"/>
        </source>
    </evidence>
    <sequence length="711" mass="81425" checksum="5413518D1425F5AF" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5U7X1</accession>
    <name>W5U7X1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ATP6V0A2</name>
        <name evidence="13" type="synonym">atp6v0a2b</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="JT407155">
        <property type="protein sequence ID" value="AHH37880.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017307352.1">
        <property type="nucleotide sequence ID" value="XM_017451863.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U7X1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000025074"/>
    <dbReference type="GeneID" id="108255708"/>
    <dbReference type="CTD" id="561117"/>
    <dbReference type="OMA" id="TYVQLYI"/>
    <dbReference type="OrthoDB" id="10264220at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026028">
        <property type="entry name" value="V-type_ATPase_116kDa_su_euka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF22">
        <property type="entry name" value="V-TYPE PROTON ATPASE 116 KDA SUBUNIT A 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001293">
        <property type="entry name" value="ATP6V0A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="409"/>
            <end position="433"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="454"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="548"/>
            <end position="566"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="578"/>
            <end position="601"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="644"/>
            <end position="667"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="779"/>
            <end position="802"/>
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    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="94"/>
            <end position="128"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AHH37880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017307352.1"/>
        </source>
    </evidence>
    <sequence length="849" mass="97587" checksum="78C72C883CC6DB05" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5U8D6</accession>
    <name>W5U8D6_ICTPU</name>
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        <submittedName>
            <fullName evidence="12">Protein IMPACT isoform X1</fullName>
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        <submittedName>
            <fullName evidence="10">Protein IMPACT-B</fullName>
        </submittedName>
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    <gene>
        <name evidence="10" type="primary">impact-B</name>
        <name evidence="12" type="synonym">impact</name>
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    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
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    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
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    <reference evidence="12" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
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    </reference>
    <comment type="subunit">
        <text evidence="7">Interacts with GCN1; prevents the interaction of GCN1 with EIF2AK4/GCN2 and inhibits EIF2AK4/GCN2 kinase activity. Interaction with RPL39; this interaction occurs in a GCN1-independent manner. Associates with ribosomes; this interaction occurs in a GCN1-independent manner. Associates with actin; this interaction occurs in a GCN1-independent manner.</text>
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        </subcellularLocation>
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        <text evidence="2">Belongs to the IMPACT family.</text>
    </comment>
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        <property type="molecule type" value="mRNA"/>
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    <dbReference type="KEGG" id="ipu:108271173"/>
    <dbReference type="CTD" id="55364"/>
    <dbReference type="GeneTree" id="ENSGT00390000017571"/>
    <dbReference type="OMA" id="FYEISAP"/>
    <dbReference type="OrthoDB" id="69641at2759"/>
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        <property type="component" value="Chromosome 10"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0140469">
        <property type="term" value="P:GCN2-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="RWD_IMPACT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IMPACT isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Impact, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Impact"/>
    </dbReference>
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        <property type="entry name" value="Impact_N"/>
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        <property type="entry name" value="Impact_N_sf"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
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        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
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    </dbReference>
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        <property type="entry name" value="UPF0029_Impact_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301">
        <property type="entry name" value="IMPACT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301:SF25">
        <property type="entry name" value="PROTEIN IMPACT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Impact_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00910">
        <property type="entry name" value="UPF0029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="RWD" evidence="9">
        <location>
            <begin position="16"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="312"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007665"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AHH37804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017334023.1"/>
        </source>
    </evidence>
    <sequence length="323" mass="36723" checksum="8D1226C02B567B9B" modified="2014-04-16" version="1">MDTITENADDLQRQVEEIEALSSIYGDEWCVIDQASRVFCIKISDDISEPKCSVCLQIILPEDYPSSSPPIYQINAVWLRGPDRITLSNSLEELYLENPGESILYLWVEKIREFLVDRSQNANAAEGLELWHAKKTEEQHDDWDEAEVPDYGVLRLSTEQTHLVSVFSSVDSDEELPVIKHGEAITDRRSTFQPHLALVVTPKQVQKVLDKLYENKKIASATHNIYAYRIYCEDKNTFLQDCEDDGETAAGGRLLHLLQILDVRNVLVVVSRWYGGILLGPDRFKHINNCARNILVQEGYTEISDEASKPGGKSKKPKSKKMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5U9P0</accession>
    <name>W5U9P0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Complexin-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">Cplx1</name>
        <name evidence="16" type="synonym">LOC108274425</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="11">Binds to the SNARE core complex containing SNAP25, VAMP2 and STX1A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Perikaryon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Presynapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complexin/synaphin family.</text>
    </comment>
    <dbReference type="EMBL" id="JT409542">
        <property type="protein sequence ID" value="AHH38721.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017340078.1">
        <property type="nucleotide sequence ID" value="XM_017484589.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U9P0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000010479"/>
    <dbReference type="GeneID" id="108274425"/>
    <dbReference type="KEGG" id="ipu:108274425"/>
    <dbReference type="GeneTree" id="ENSGT00950000182938"/>
    <dbReference type="OMA" id="MGATKDM"/>
    <dbReference type="OrthoDB" id="5972090at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043195">
        <property type="term" value="C:terminal bouton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046928">
        <property type="term" value="P:regulation of neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016079">
        <property type="term" value="P:synaptic vesicle exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22740">
        <property type="entry name" value="Complexin_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.580:FF:000001">
        <property type="entry name" value="Complexin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.580">
        <property type="entry name" value="Single Helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008849">
        <property type="entry name" value="Synaphin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16705">
        <property type="entry name" value="COMPLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16705:SF6">
        <property type="entry name" value="COMPLEXIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05835">
        <property type="entry name" value="Synaphin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="7" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="74"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="15"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AHH38721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017340078.1"/>
        </source>
    </evidence>
    <sequence length="134" mass="15199" checksum="9B51B67A4CF55425" modified="2014-04-16" version="1">MNFVMKQALGGATKDMGKMLGEKEEKDPDAEKKEEERQEALRQQEEERKAKYAKMEAERESLRQGIRDKYGIKKKEEKEAEAQAAMEQASEGSLTRPKKAVPAGCGDDEDEEESIVDTVMKFLPGPLLDMFNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5U9P4</accession>
    <name>W5U9P4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">EIF4A2</name>
        <name evidence="19" type="synonym">eif4a2</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. eIF4A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JT408181">
        <property type="protein sequence ID" value="AHH38259.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017327800.1">
        <property type="nucleotide sequence ID" value="XM_017472311.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U9P4"/>
    <dbReference type="GeneID" id="108267852"/>
    <dbReference type="KEGG" id="ipu:108267852"/>
    <dbReference type="CTD" id="1974"/>
    <dbReference type="GeneTree" id="ENSGT00940000153783"/>
    <dbReference type="OMA" id="FGCQALV"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18046">
        <property type="entry name" value="DEADc_EIF4AII_EIF4AI_DDX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000089">
        <property type="entry name" value="Eukaryotic initiation factor 4A-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000031">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044728">
        <property type="entry name" value="EIF4A_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 17 19" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="16">
        <location>
            <begin position="37"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="68"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="250"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="11">
        <location>
            <begin position="37"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AHH38259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017327800.1"/>
        </source>
    </evidence>
    <sequence length="411" mass="46839" checksum="37373144DC28A90C" modified="2014-04-16" version="1">MSSGSADYHSSRDRDHGGPEGMEPDGIIESNWNEITDNFDDMNLKETLLRGIYAYGFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEQKETQALVLAPTRELAQQIQKVILALGDYMGASCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRFLSPKWIKMFVLDEADEMLSRGFKDQIYEIFQKLSTNIQVVLLSATMPAEVLDVTTKFMREPVRILVKKEELTLEGIKQFYINVEREEWKLDTLCDLYETLTITQAVIFLNTRRKVDWLTEKMHARDFTVSALHGDMDQKERDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINFVTEEDKRILRDIETFYNTTVEEMPMNVADLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5UC39</accession>
    <name>W5UC39_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Electron transfer flavoprotein subunit beta</fullName>
            <shortName evidence="8">Beta-ETF</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ETFB</name>
        <name evidence="12" type="synonym">etfb</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase. Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism. ETFB binds an AMP molecule that probably has a purely structural role.</text>
    </comment>
    <comment type="function">
        <text evidence="8">The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimer composed of ETFA and ETFB. Identified in a complex that contains ETFA, ETFB and ETFRF1. Interacts with ACADM.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ETF beta-subunit/FixA family.</text>
    </comment>
    <dbReference type="EMBL" id="JT411635">
        <property type="protein sequence ID" value="AHH39528.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017308858.1">
        <property type="nucleotide sequence ID" value="XM_017453369.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UC39"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034670"/>
    <dbReference type="GeneID" id="108256461"/>
    <dbReference type="KEGG" id="ipu:108256461"/>
    <dbReference type="CTD" id="2109"/>
    <dbReference type="GeneTree" id="ENSGT00390000009936"/>
    <dbReference type="OMA" id="EINQPRI"/>
    <dbReference type="OrthoDB" id="276685at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045251">
        <property type="term" value="C:electron transfer flavoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01714">
        <property type="entry name" value="ETF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000011">
        <property type="entry name" value="Electron transfer flavoprotein subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000049">
        <property type="entry name" value="ET-Flavoprotein_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012255">
        <property type="entry name" value="ETF_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033948">
        <property type="entry name" value="ETF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN BETA-SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294:SF8">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000090">
        <property type="entry name" value="Beta-ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01065">
        <property type="entry name" value="ETF_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="9">
        <location>
            <begin position="25"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AHH39528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017308858.1"/>
        </source>
    </evidence>
    <sequence length="254" mass="27515" checksum="5D8424C42A60C360" modified="2014-04-16" version="1">MTGRVLVGVKRVIDYAVKIRVKPDKTGVVTDGVKHSMNPFCEIAVEEAVRLKEKKLVKEIVAVSCGPQQVQETIRTALAMGADRGIHVEVSGKDYESLGPLQVSKILAALAKNEDASLIILGKQAIDDDCNQTGQMTAALLDWPQGTFASEVTFEGDKLKVVREIDGGLETIKINTPAVVTADLRLNTPRYATLPNIMKAKKKKIASVKPGDLGVDMSSRMEVLQVDEPPQRQAGMKVESVEELVAKLKEAGNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5UES7</accession>
    <name>W5UES7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">CYFIP-related Rac1 interactor B</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="6">Protein FAM49B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">FAM49B</name>
        <name evidence="8 9" type="synonym">fam49ba</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8 9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CYRI family.</text>
    </comment>
    <dbReference type="EMBL" id="JT412993">
        <property type="protein sequence ID" value="AHH40074.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017327649.1">
        <property type="nucleotide sequence ID" value="XM_017472160.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017327650.1">
        <property type="nucleotide sequence ID" value="XM_017472161.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UES7"/>
    <dbReference type="GeneID" id="108267758"/>
    <dbReference type="KEGG" id="ipu:108267758"/>
    <dbReference type="CTD" id="337591"/>
    <dbReference type="OMA" id="PSETDIW"/>
    <dbReference type="OrthoDB" id="60973at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039789">
        <property type="entry name" value="CYRI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009828">
        <property type="entry name" value="CYRIA/CYRIB_Rac1-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12422">
        <property type="entry name" value="GH09096P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07159">
        <property type="entry name" value="CYRIA-B_Rac1-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CYRIA/CYRIB Rac1 binding" evidence="5">
        <location>
            <begin position="18"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AHH40074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017327649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017327650.1"/>
        </source>
    </evidence>
    <sequence length="325" mass="36853" checksum="ED354496E84E4F4B" modified="2014-04-16" version="1">MGNLIKVLTRDIDNNAGNFFLDFENAQPTDAERELWEQVNKVLTEAVSVLQDLQSYSGAGESIRQAIQQPSNESVQEKAWTAVVPLVGKLKKFYEFSLKLEGALHGLLGFLTSAHCSPTQHLEQEQALARQFAEILHFTLRFDELKMTNPAIQNDFSYYRRTLSRMRINNLSTEEENEVNNELANRMSLFYANATPMLKTLSDATTKFVSENSDVPIENTTDCLSMMACVCKVMLDTPEYRSRFASEDTVLFCLRVMVGVIILYDYVHPAGAFVKSSKIDMKGCIKVLRDQPPNRVEGLLNALRYTTKHLNDETTSKQIKNMLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5UEW9</accession>
    <name>W5UEW9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cysteine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="4">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Cysteinyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">Cars</name>
        <name evidence="21" type="synonym">cars1</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the ATP-dependent ligation of cysteine to tRNA(Cys).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>tRNA(Cys) + L-cysteine + ATP = L-cysteinyl-tRNA(Cys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17773"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:17774"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="4"/>
    <dbReference type="EMBL" id="JT414273">
        <property type="protein sequence ID" value="AHH40561.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017314790.1">
        <property type="nucleotide sequence ID" value="XM_017459301.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UEW9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026578"/>
    <dbReference type="GeneID" id="108259655"/>
    <dbReference type="KEGG" id="ipu:108259655"/>
    <dbReference type="CTD" id="833"/>
    <dbReference type="OrthoDB" id="438179at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10310">
        <property type="entry name" value="GST_C_CysRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000027">
        <property type="entry name" value="Cysteine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1910:FF:000005">
        <property type="entry name" value="Cysteine-tRNA ligase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000228">
        <property type="entry name" value="Cysteinyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890:SF3">
        <property type="entry name" value="CYSTEINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="CYSTEINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 19 21" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA synthetases class I catalytic" evidence="18">
        <location>
            <begin position="118"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="731"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="731"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="767"/>
            <end position="776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AHH40561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017314790.1"/>
        </source>
    </evidence>
    <sequence length="822" mass="93820" checksum="32A6F80EE98C3941" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5UKP3</accession>
    <name>W5UKP3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carbonic anhydrase</fullName>
            <ecNumber evidence="4">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Ca5b</name>
        <name evidence="8" type="synonym">ca5a</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="4"/>
    <dbReference type="EMBL" id="JT417125">
        <property type="protein sequence ID" value="AHH42265.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017329757.1">
        <property type="nucleotide sequence ID" value="XM_017474268.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UKP3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020967"/>
    <dbReference type="GeneID" id="108268921"/>
    <dbReference type="KEGG" id="ipu:108268921"/>
    <dbReference type="CTD" id="763"/>
    <dbReference type="OrthoDB" id="429145at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018338">
        <property type="entry name" value="Carbonic_anhydrase_a-class_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF25">
        <property type="entry name" value="CARBONIC ANHYDRASE 5B, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00162">
        <property type="entry name" value="ALPHA_CA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="5">
        <location>
            <begin position="36"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AHH42265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017329757.1"/>
        </source>
    </evidence>
    <sequence length="307" mass="35396" checksum="1D093BC84E6346E1" modified="2014-04-16" version="1">MVALTVLARHLQRNLVRQLRIPRLSVPVRRCNLSACSSRYVRSQLHPMWQEPLEMPGGERQSPIDISLRKSVFDPNLKPFVTQYDPRTCQQIWNNGYSFLVEYDDTTDKSTLRGGPLEDKFRLCQFHFHWGENNTWGSEHTVDRHLYPAELHLVHWNADKYALFEEAIMEENGLAVIGVFLKVGKRHEGLQKLVDALPAVRHKDSVVEFTKFDPSCLLPESMNEYWTYAGSLTTPPLTEAVTWIIMKQAIEVSHEQLAVFRSLLFTSAEEEVQKSMVNNFRVQQPLKGRTVRSSFSPFLENVPSTDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5UMA1</accession>
    <name>W5UMA1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">gamma-butyrobetaine dioxygenase</fullName>
            <ecNumber evidence="6">1.14.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Gamma-butyrobetaine hydroxylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Gamma-butyrobetaine,2-oxoglutarate dioxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Bbox1</name>
        <name evidence="19" type="synonym">bbox1</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the formation of L-carnitine from gamma-butyrobetaine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>4-(trimethylamino)butanoate + 2-oxoglutarate + O2 = carnitine + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:24028"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16244"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17126"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="EC" id="1.14.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amine and polyamine biosynthesis; carnitine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the gamma-BBH/TMLD family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.1" evidence="6"/>
    <dbReference type="EMBL" id="JT414868">
        <property type="protein sequence ID" value="AHH40787.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017314146.1">
        <property type="nucleotide sequence ID" value="XM_017458657.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UMA1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029174"/>
    <dbReference type="GeneID" id="108259295"/>
    <dbReference type="KEGG" id="ipu:108259295"/>
    <dbReference type="CTD" id="8424"/>
    <dbReference type="GeneTree" id="ENSGT00530000063582"/>
    <dbReference type="OMA" id="VHITWPN"/>
    <dbReference type="OrthoDB" id="406634at2759"/>
    <dbReference type="UniPathway" id="UPA00118"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008336">
        <property type="term" value="F:gamma-butyrobetaine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045329">
        <property type="term" value="P:carnitine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00250">
        <property type="entry name" value="CAS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2020.30:FF:000002">
        <property type="entry name" value="Putative gamma-butyrobetaine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.10:FF:000001">
        <property type="entry name" value="Trimethyllysine dioxygenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2020.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050411">
        <property type="entry name" value="AlphaKG_dependent_hydroxylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012775">
        <property type="entry name" value="GBBH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010376">
        <property type="entry name" value="GBBH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038492">
        <property type="entry name" value="GBBH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02409">
        <property type="entry name" value="carnitine_bodg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696:SF33">
        <property type="entry name" value="GAMMA-BUTYROBETAINE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696">
        <property type="entry name" value="GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06155">
        <property type="entry name" value="GBBH-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-0124">Carnitine biosynthesis</keyword>
    <keyword evidence="9 17 19" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma-butyrobetaine hydroxylase-like N-terminal" evidence="16">
        <location>
            <begin position="58"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="TauD/TfdA-like" evidence="15">
        <location>
            <begin position="173"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF06155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AHH40787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017314146.1"/>
        </source>
    </evidence>
    <sequence length="434" mass="49553" checksum="1113A7CC4495FD66" modified="2014-04-16" version="1">MFSSLARCASRAALHVLKGTSRPSKPGKVPCFGHQTLAAVPLPTVGGTTVVRHAQVLDHKRLLQVEWDDGSYSFYPFTWLRDNCQCPLCMLQSAKARSLLLTDLDVHTGMDQVQITENNKVSITWPDQHSSEFEPDWLKKRCFSPEARQARQEELFLNEREFWDSALQIPMADFEAVLHDDKAALAWLVALRRFGIVYLKGAPVEQGQLARLSRRIGYLRLTFYGHTWQVQDKPMANNVAYTSGKLSLHTDYPALHHPPGVQFLHCLQQAQQGGESEVVDGFHMAELLRREDPEAFNTLSSLYIDYTDTGSDYCDFIVQSKNRIIDVDCEGRVVRINYNNATRDSTLDLPLDQVQPFYSSLKSFVELMNRPENVVSYRMEPGDLVTFDNWRLLHGRKNYSSGGRTLRHLEGAYLDWDEVMSRMRILRKAVHGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>W5UMH6</accession>
    <name>W5UMH6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Four and a half LIM domains protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">Fhl1</name>
        <name evidence="19 20" type="synonym">fhl1b</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19 20" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19 20">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">May have an involvement in muscle development or hypertrophy. Isoform 2 binds to RBP-J and plays a negative regulatory role in the RBP-J-mediated transcription in mammalian systems.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT418971">
        <property type="protein sequence ID" value="AHH42915.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053542573.1">
        <property type="nucleotide sequence ID" value="XM_053686598.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053542574.1">
        <property type="nucleotide sequence ID" value="XM_053686599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UMH6"/>
    <dbReference type="KEGG" id="ipu:108276810"/>
    <dbReference type="OrthoDB" id="1112565at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09344">
        <property type="entry name" value="LIM1_FHL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09348">
        <property type="entry name" value="LIM4_FHL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000013">
        <property type="entry name" value="Four and a half LIM domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000052">
        <property type="entry name" value="Four and a half LIM domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000050">
        <property type="entry name" value="Four and a half LIM domains protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000072">
        <property type="entry name" value="Four and a half LIM domains protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042997">
        <property type="entry name" value="Fhl1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47029">
        <property type="entry name" value="FOUR AND A HALF LIM DOMAINS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47029:SF2">
        <property type="entry name" value="FOUR AND A HALF LIM DOMAINS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12 15" id="KW-0440">LIM domain</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="16">
        <location>
            <begin position="39"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="16">
        <location>
            <begin position="101"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="16">
        <location>
            <begin position="220"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00074675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AHH42915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_053542573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_053542574.1"/>
        </source>
    </evidence>
    <sequence length="281" mass="32085" checksum="235518BC802E6DF3" modified="2014-04-16" version="1">MSDRFSCFYCRDDLSGKKFMQKEDNKPMCVRCFDKFCANNCAECRRPISTDSKELHYKGRYWHEDCFRCSKCYKNLAKESFSVKDDRILCGKCSSREDAPRCHACYKPILAGAESVEYKGNVFHDDCFTCYKCKKPIGSESFITKNENVYCSSCHESKFSKHCTACRKPITAGGVNYQDQPWHSECLVCVVCVKPLAGTRFTSHEEKLYCVDCYKTNVAKKCSSCQNPITGFGKATNVVNYEGGTWHDYCFTCKKCAVNLAEKRFISKDGNIYCSDCAKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5UQ75</accession>
    <name>W5UQ75_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11 13">Chondrolectin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Chodl</name>
        <name evidence="13" type="synonym">chodl</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT415469">
        <property type="protein sequence ID" value="AHH41708.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017308166.1">
        <property type="nucleotide sequence ID" value="XM_017452677.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UQ75"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020242"/>
    <dbReference type="GeneID" id="108256117"/>
    <dbReference type="CTD" id="140578"/>
    <dbReference type="OMA" id="FICKYKS"/>
    <dbReference type="OrthoDB" id="5797898at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050772">
        <property type="term" value="P:positive regulation of axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000006">
        <property type="entry name" value="Layilin b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051505">
        <property type="entry name" value="C-type_lectin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14789:SF1">
        <property type="entry name" value="CHONDROLECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14789">
        <property type="entry name" value="CHONDROLECTIN VARIANT CHODLFDELTAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0430">Lectin</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540403" evidence="9">
        <location>
            <begin position="30"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="236"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="10">
        <location>
            <begin position="41"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AHH41708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017308166.1"/>
        </source>
    </evidence>
    <sequence length="293" mass="32593" checksum="1D137BDE9B8B45E5" modified="2014-04-16" version="1">MRAESTALGILYGLTVWVASCRGARVVSGQTVCHGGPEKPCYKIAYFSDVSSRVAFWEAQQVCEVDGGSLLSVENFTEQRHIEQLLQNLSRTSSGPGIADGDFWIGLTRIDRGSPGEYASYASCPDLYSWMDGSVSQFRNWYFDEPSCGAEACVVMYHQPTAPAGLGGSYLYQWNDDRCSMKHNFICKYEPESHLVKEHGDRPVSRDAEHEEETKTPDVYEEESLHVTVAGHSSMLLIYVIIPTIPLLLLILVASGTCCVQMLSKRRPQTKTRVDQSTLWISKTPKSDSAMEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5UR71</accession>
    <name>W5UR71_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Claudin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CLDN11</name>
        <name evidence="14" type="synonym">cldn11b</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with tetraspanin-3/TSPAN3. Interacts with OCLN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell junction</location>
            <location evidence="11">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="EMBL" id="JT416620">
        <property type="protein sequence ID" value="AHH42098.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017309321.1">
        <property type="nucleotide sequence ID" value="XM_017453832.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UR71"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000025430"/>
    <dbReference type="GeneID" id="108256695"/>
    <dbReference type="KEGG" id="ipu:108256695"/>
    <dbReference type="CTD" id="81592"/>
    <dbReference type="OMA" id="MAHICRQ"/>
    <dbReference type="OrthoDB" id="9411914at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003555">
        <property type="entry name" value="Claudin11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01384">
        <property type="entry name" value="CLAUDIN11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7 11" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0796">Tight junction</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="83"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU060637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AHH42098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017309321.1"/>
        </source>
    </evidence>
    <sequence length="217" mass="23137" checksum="60B765BF0A349F97" modified="2014-04-16" version="1">MSQSCRLLCGFFLSCSGWICLIIATSTNDWVITCSFGLHTCVKMDELESKGLWAECMISTAAYHCAALNQILTLPAYIQTSRALMVSASLLGLPSVLLALLATPCVRLGDESDGTKQKRALLGGILTLIMSVCGLVSTVWFPIGAHHEDGLLSFGFSLYAGWLGSALCFLGGSVLTCCSGGNNAPAQRPEKRFYYSKQSGITDSVQATNNHAKSAHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PIT0</accession>
    <name>A0A2D0PIT0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Telomeric repeat-binding factor 2-interacting protein 1</fullName>
            <shortName evidence="10">TERF2-interacting telomeric protein 1</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">Repressor/activator protein 1 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">terf2ip</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Acts both as a regulator of telomere function and as a transcription regulator. Involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). Does not bind DNA directly: recruited to telomeric double-stranded 5'-TTAGGG-3' repeats via its interaction with terf2. Independently of its function in telomeres, also acts as a transcription regulator: recruited to extratelomeric 5'-TTAGGG-3' sites via its association with terf2 or other factors, and regulates gene expression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Chromosome</location>
            <location evidence="10">Telomere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the RAP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017305832.1">
        <property type="nucleotide sequence ID" value="XM_017450343.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PIT0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000011391"/>
    <dbReference type="GeneID" id="108254903"/>
    <dbReference type="KEGG" id="ipu:108254903"/>
    <dbReference type="CTD" id="54386"/>
    <dbReference type="OMA" id="MRFFIRP"/>
    <dbReference type="OrthoDB" id="435460at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070187">
        <property type="term" value="C:shelterin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031848">
        <property type="term" value="P:protection from non-homologous end joining at telomere"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010833">
        <property type="term" value="P:telomere maintenance via telomere lengthening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11655">
        <property type="entry name" value="rap1_myb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000246">
        <property type="entry name" value="Telomeric repeat-binding factor 2-interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021661">
        <property type="entry name" value="Rap1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038104">
        <property type="entry name" value="Rap1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039595">
        <property type="entry name" value="TE2IP/Rap1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015010">
        <property type="entry name" value="TERF2IP_Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16466">
        <property type="entry name" value="TELOMERE REPEAT-BINDING FACTOR 2-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16466:SF6">
        <property type="entry name" value="TELOMERIC REPEAT-BINDING FACTOR 2-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08914">
        <property type="entry name" value="Myb_Rap1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11626">
        <property type="entry name" value="Rap1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0010">Activator</keyword>
    <keyword evidence="3 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0779">Telomere</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BRCT" evidence="14">
        <location>
            <begin position="15"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="TERF2-interacting telomeric protein 1 Myb" evidence="12">
        <location>
            <begin position="123"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="TRF2-interacting telomeric protein/Rap1 C-terminal" evidence="13">
        <location>
            <begin position="465"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="176"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="176"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="274"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="300"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="336"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="367"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF11626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF16589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017305832.1"/>
        </source>
    </evidence>
    <sequence length="536" mass="59838" checksum="8F719D0956B09841" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PJH1</accession>
    <name>A0A2D0PJH1_ICTPU</name>
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        <recommendedName>
            <fullName evidence="14">Regulator of microtubule dynamics protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Protein FAM82A2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Protein FAM82C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20 21" type="primary">rmdn3</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20 21" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20 21">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle pole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the RMDN family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017451259.3"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_017451267.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PJH1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000023485"/>
    <dbReference type="GeneID" id="108255339"/>
    <dbReference type="KEGG" id="ipu:108255339"/>
    <dbReference type="CTD" id="55177"/>
    <dbReference type="GeneTree" id="ENSGT00950000182992"/>
    <dbReference type="OrthoDB" id="512473at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097431">
        <property type="term" value="C:mitotic spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049039">
        <property type="entry name" value="RMD1-3_a_helical_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16056">
        <property type="entry name" value="REGULATOR OF MICROTUBULE DYNAMICS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16056:SF18">
        <property type="entry name" value="REGULATOR OF MICROTUBULE DYNAMICS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21033">
        <property type="entry name" value="RMD1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="155"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="187"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="292"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017306748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017306756.1"/>
        </source>
    </evidence>
    <sequence length="470" mass="52592" checksum="9833294BF4FA9661" modified="2017-12-20" version="1">MSSLGRNGLIGFGIGVTVSTGMIAFLIIKEMMRRRSERHLQVSRSSAGFAVSSAAVDGRINLEPDSREVLASALSGLTPEQHAELRKSLDEVMMSVSTLRSEVAELRNGLGDIASTIIEDIRKGVEEGQRSRRRRHFLSRERSDSMSSSSIYFTASNGMSSRSEGGYTTAYSTAESDYTDRETDRDEDRDEDDEDEEEERSCATVLTLQPDELQSEQDEVDGDEEAMMRSPELALLLSQSDILHAGDSGKKTDGFYLLRENKPLYSDNVEFLWRLARAYNDMCEHAEDEEERQSYAEQGRDEAESALQSNGLNADCHKWFAVLTGQTLQCDSMHGKLKSSHILKEHLDRALALRDDDPLCFYLLGRWCYEMSRLSWLEQKAAAALYETPPCSSLHHALENFLKAEELKPGFSRAVRLYVAKCHKDLGNESEARNWAHLALATPTNTHQEASISGLEAELQALIDSPAEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PKI7</accession>
    <name>A0A2D0PKI7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Solute carrier family 41 member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">slc41a1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as a magnesium transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SLC41A transporter family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017305926.1">
        <property type="nucleotide sequence ID" value="XM_017450437.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PKI7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006290"/>
    <dbReference type="GeneID" id="108254937"/>
    <dbReference type="KEGG" id="ipu:108254937"/>
    <dbReference type="CTD" id="254428"/>
    <dbReference type="OrthoDB" id="5791097at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022890">
        <property type="term" value="F:inorganic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043009">
        <property type="term" value="P:chordate embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010960">
        <property type="term" value="P:magnesium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048793">
        <property type="term" value="P:pronephros development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.20:FF:000001">
        <property type="entry name" value="Solute carrier family 41 member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.20:FF:000002">
        <property type="entry name" value="Solute carrier family 41, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.20">
        <property type="entry name" value="SLC41 divalent cation transporters, integral membrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006667">
        <property type="entry name" value="SLC41_membr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036739">
        <property type="entry name" value="SLC41_membr_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045349">
        <property type="entry name" value="SLC41A1-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228">
        <property type="entry name" value="DIVALENT CATION TRANSPORTER SOLUTE CARRIER FAMILY 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228:SF23">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 41 MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01769">
        <property type="entry name" value="MgtE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161093">
        <property type="entry name" value="MgtE membrane domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="202"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="237"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="375"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="462"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="507"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="SLC41A/MgtE integral membrane" evidence="11">
        <location>
            <begin position="165"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="SLC41A/MgtE integral membrane" evidence="11">
        <location>
            <begin position="379"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017305926.1"/>
        </source>
    </evidence>
    <sequence length="539" mass="58292" checksum="54F2E9970321964E" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PQN7</accession>
    <name>A0A2D0PQN7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Polyadenylate-binding protein 1A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC108255735</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the polyadenylate-binding protein type-1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017307395.3">
        <property type="nucleotide sequence ID" value="XM_017451906.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PQN7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009876"/>
    <dbReference type="GeneID" id="108255735"/>
    <dbReference type="KEGG" id="ipu:108255735"/>
    <dbReference type="OrthoDB" id="19742at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12379">
        <property type="entry name" value="RRM2_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.10">
        <property type="entry name" value="c-terminal domain of poly(a) binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036053">
        <property type="entry name" value="PABP-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045305">
        <property type="entry name" value="RRM2_I_PABPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052462">
        <property type="entry name" value="SLIRP/GR-RBP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48027">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN 87F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00658">
        <property type="entry name" value="MLLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00517">
        <property type="entry name" value="PolyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63570">
        <property type="entry name" value="PABC (PABP) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="7"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="95"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="PABC" evidence="9">
        <location>
            <begin position="385"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="334"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017307395.3"/>
        </source>
    </evidence>
    <sequence length="503" mass="56136" checksum="EB4C62A69A459DDD" modified="2023-06-28" version="3">MSHKMTAALYVGGLHPEVTEEMLARTFSHAGRIHSIRICRDWKTRSSRGYAFVNFQQRADAERAIEMFHSELLMGRPMCVQWSQRDSSLRNSSIGKLFIKNLDESIDTLALLDTFSAFGKVLSCKVVGDENGSKGYGYVHFESAEAADFAMNRLNGKLLNDRKVSIEHFKSREEREAQAGRWGRKPVYVGVAQQKEECQVYPAHQNRQRMASMQAQNLAVSYTPCQKVEPHTDLLWTAQTIQSQHVQNIPDVIQPAPVHQQTGSTITSTSQAPCMNEECITIITPTTDGTVPVADTVPAVETVPPVDSVPAVDTLPGADTLPSLESVPLVETVPPVDSVSDLGSTADEVPAPPQAPDAAEMPSTSISEVQQHKDTTDVATYPPAGKRLTIYMLESSRLDDQMQMAHDHLLPLIEEIHPTQANKITWMLLQDANNFEVIDMIAYPELLRTKVDEMDALLKAREAGYKPETLKMSLNNNNNKKKNNKKRKNKYITINHCNQYCTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PTL9</accession>
    <name>A0A2D0PTL9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Centrosomal protein of 85 kDa isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cep85</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017308440.2">
        <property type="nucleotide sequence ID" value="XM_017452951.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PTL9"/>
    <dbReference type="GeneID" id="108256287"/>
    <dbReference type="KEGG" id="ipu:108256287"/>
    <dbReference type="CTD" id="64793"/>
    <dbReference type="OrthoDB" id="5972981at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058190">
        <property type="entry name" value="CC4_CEP85"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040210">
        <property type="entry name" value="Cep85/Cep85L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31075">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 85 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31075:SF3">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 85 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24555">
        <property type="entry name" value="CC4_CEP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Centrosomal protein of 85 kDa-like CC4 coiled-coil" evidence="3">
        <location>
            <begin position="544"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="53"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="97"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="306"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="396"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="539"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017308440.2"/>
        </source>
    </evidence>
    <sequence length="734" mass="82534" checksum="E8F49FBBFA291954" modified="2023-02-22" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PWL2</accession>
    <name>A0A2D0PWL2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Elongation factor 1-alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC108256978</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017309806.1">
        <property type="nucleotide sequence ID" value="XM_017454317.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PWL2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029480"/>
    <dbReference type="GeneID" id="108256978"/>
    <dbReference type="KEGG" id="ipu:108256978"/>
    <dbReference type="OrthoDB" id="342024at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000090">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000168">
        <property type="entry name" value="Elongation factor 1-alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_A">
        <property type="entry name" value="EF_Tu_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008969">
        <property type="entry name" value="PRK12317.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 11" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="7 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="5"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017309806.1"/>
        </source>
    </evidence>
    <sequence length="462" mass="50225" checksum="5FDE741252AEFBA5" modified="2017-12-20" version="1">MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYITIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSQARFEEITKEVGAYIKKIGYNPAAVAFVPISGWHGDNMLEPSTNMTWFKGWKIERKEGAANGTTLLEALDSILPPSRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGMLKPGMVVTFAPVNVTTEVKSVEMHHESLAEALPGDNVGFNVKNVSVKDIRRGNVAGDSKNDPPQQAGNFTAQVIILNHPGQISQGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDNPKNLKSGDAAIILMIPGKPMCVESFSQYPPLGRFAVRDMRQTVAVGVIKAVDKKASTAGKVTKSAQKAAKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PXW6</accession>
    <name>A0A2D0PXW6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Iodothyronine deiodinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="primary">dio3b</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="29">SDA103</strain>
        </source>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="30">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="27">Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>3',5'-diiodo-L-thyronine + iodide + A + H(+) = 3,3',5'-triiodo-L-thyronine + AH2</text>
            <dbReference type="Rhea" id="RHEA:83775"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:57261"/>
            <dbReference type="ChEBI" id="CHEBI:195762"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="23">
            <dbReference type="Rhea" id="RHEA:83777"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>3',5'-diiodothyronamine + iodide + A + H(+) = 3,3',5'-triiodothyronamine + AH2</text>
            <dbReference type="Rhea" id="RHEA:83799"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:233342"/>
            <dbReference type="ChEBI" id="CHEBI:233343"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="21">
            <dbReference type="Rhea" id="RHEA:83801"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>3'-iodo-L-thyronine + iodide + A + H(+) = 3,3'-diiodo-L-thyronine + AH2</text>
            <dbReference type="Rhea" id="RHEA:83779"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:176514"/>
            <dbReference type="ChEBI" id="CHEBI:232695"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="22">
            <dbReference type="Rhea" id="RHEA:83781"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>3'-iodothyronamine + iodide + A + H(+) = 3,3'-diiodothyronamine + AH2</text>
            <dbReference type="Rhea" id="RHEA:83815"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:233339"/>
            <dbReference type="ChEBI" id="CHEBI:233341"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:83817"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>3,3',5'-triiodo-L-thyronine + iodide + A + H(+) = L-thyroxine + AH2</text>
            <dbReference type="Rhea" id="RHEA:18897"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:57261"/>
            <dbReference type="ChEBI" id="CHEBI:58448"/>
            <dbReference type="EC" id="1.21.99.3"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:18899"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>3,3',5'-triiodothyronamine + iodide + A + H(+) = 3,3',5,5'-tetraiodothyronamine + AH2</text>
            <dbReference type="Rhea" id="RHEA:83807"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:233343"/>
            <dbReference type="ChEBI" id="CHEBI:233344"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="20">
            <dbReference type="Rhea" id="RHEA:83809"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>3,3'-diiodo-L-thyronine + iodide + A + H(+) = 3,3',5-triiodo-L-thyronine + AH2</text>
            <dbReference type="Rhea" id="RHEA:82571"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:176514"/>
            <dbReference type="ChEBI" id="CHEBI:533015"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:82573"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>3,3'-diiodothyronamine + iodide + A + H(+) = 3,3',5-triiodothyronamine + AH2</text>
            <dbReference type="Rhea" id="RHEA:83811"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:233341"/>
            <dbReference type="ChEBI" id="CHEBI:233426"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="18">
            <dbReference type="Rhea" id="RHEA:83813"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>3-iodo-L-thyronine + iodide + A + H(+) = 3,5-diiodo-L-thyronine + AH2</text>
            <dbReference type="Rhea" id="RHEA:82895"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:232626"/>
            <dbReference type="ChEBI" id="CHEBI:232627"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="24">
            <dbReference type="Rhea" id="RHEA:82897"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>3-iodothyronamine + iodide + A + H(+) = 3,5-diiodothyronamine + AH2</text>
            <dbReference type="Rhea" id="RHEA:83823"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:231647"/>
            <dbReference type="ChEBI" id="CHEBI:233340"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:83825"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-thyronine + iodide + A + H(+) = 3-iodo-L-thyronine + AH2</text>
            <dbReference type="Rhea" id="RHEA:83771"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:232627"/>
            <dbReference type="ChEBI" id="CHEBI:233333"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="19">
            <dbReference type="Rhea" id="RHEA:83773"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>thyronamine + iodide + A + H(+) = 3-iodothyronamine + AH2</text>
            <dbReference type="Rhea" id="RHEA:83819"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:231647"/>
            <dbReference type="ChEBI" id="CHEBI:233334"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="25">
            <dbReference type="Rhea" id="RHEA:83821"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Monomer. Homodimer. May undergo minor heretodimerization with DIO1 and DIO2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 27">Belongs to the iodothyronine deiodinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017310884.1">
        <property type="nucleotide sequence ID" value="XM_017455395.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000033824"/>
    <dbReference type="GeneID" id="108257532"/>
    <dbReference type="KEGG" id="ipu:108257532"/>
    <dbReference type="CTD" id="798872"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004800">
        <property type="term" value="F:thyroxine 5'-deiodinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033798">
        <property type="term" value="F:thyroxine 5-deiodinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042446">
        <property type="term" value="P:hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042403">
        <property type="term" value="P:thyroid hormone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000239">
        <property type="entry name" value="Iodothyronine deiodinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000643">
        <property type="entry name" value="Iodothyronine_deiodinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008261">
        <property type="entry name" value="Iodothyronine_deiodinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027252">
        <property type="entry name" value="Iodothyronine_deiodinase_I/III"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11781">
        <property type="entry name" value="IODOTHYRONINE DEIODINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11781:SF4">
        <property type="entry name" value="THYROXINE 5-DEIODINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00837">
        <property type="entry name" value="T4_deiodinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001330">
        <property type="entry name" value="IOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500144">
        <property type="entry name" value="IODI_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01205">
        <property type="entry name" value="T4_DEIODINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="12 28" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 27" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="30" id="KW-0711">Selenium</keyword>
    <keyword evidence="8 27 30" id="KW-0712">Selenocysteine</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 27" id="KW-0893">Thyroid hormones biosynthesis</keyword>
    <keyword evidence="6 28" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 28" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="active site" evidence="26">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="30">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00065768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00093185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00093211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00093217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00093239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00093249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00093252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00093262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00093269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00093272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU000676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="RefSeq" id="XP_017310884.1"/>
        </source>
    </evidence>
    <sequence length="280" mass="31668" checksum="8DEBC5705DB7B4BA" modified="2017-12-20" version="1">MEGGDSSVCAPARRMPDVQDFVKALKNALVSLMLLPRFLLTAVLLWFLDFVCIRRKVLVKMREREGSSPDDPSVTVSDSNRMFTVESLRAVWYSQKLDFCKAAHLGLTAPNSEVVPLGDRKRARILDYARGSRPLILNFGSCSUPPFMTRLAAFRRVADQYADIADSLLIYIEEAHPSDGWVSTDAPYQIPRHRCIKDRLRAARLMTATVPGSIVVVDTMDNSSNAAYGAYFERLYVVKDEKVVYQGGRGPEGYRISELRNWLEQYRQKLEGSRPIVVHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q3K5</accession>
    <name>A0A2D0Q3K5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Interferon-induced GTP-binding protein Mx2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC108258629</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017312877.1">
        <property type="nucleotide sequence ID" value="XM_017457388.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q3K5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006966"/>
    <dbReference type="GeneID" id="108258629"/>
    <dbReference type="KEGG" id="ipu:108258629"/>
    <dbReference type="OMA" id="EFHKWSA"/>
    <dbReference type="OrthoDB" id="5061070at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016185">
        <property type="term" value="P:synaptic vesicle budding from presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1240:FF:000007">
        <property type="entry name" value="Interferon-induced GTP-binding protein Mx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000621">
        <property type="entry name" value="Interferon-induced GTP-binding protein Mx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003130">
        <property type="entry name" value="GED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF225">
        <property type="entry name" value="INTERFERON-INDUCED GTP-BINDING PROTEIN MX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02212">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00302">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="8">
        <location>
            <begin position="31"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="7">
        <location>
            <begin position="539"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="510"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="567"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="510"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017312877.1"/>
        </source>
    </evidence>
    <sequence length="625" mass="72082" checksum="D2EE6C2F3F84604D" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q9A1</accession>
    <name>A0A2D0Q9A1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">arylamine N-acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC108259370</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the arylamine N-acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.5" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017314286.1">
        <property type="nucleotide sequence ID" value="XM_017458797.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q9A1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000023449"/>
    <dbReference type="GeneID" id="108259370"/>
    <dbReference type="KEGG" id="ipu:108259370"/>
    <dbReference type="OrthoDB" id="10260017at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004060">
        <property type="term" value="F:arylamine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2140.20:FF:000001">
        <property type="entry name" value="Arylamine N-acetyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2140.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001447">
        <property type="entry name" value="Arylamine_N-AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053710">
        <property type="entry name" value="Arylamine_NAT_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786:SF8">
        <property type="entry name" value="ARYLAMINE N-ACETYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786">
        <property type="entry name" value="N-HYDROXYARYLAMINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00797">
        <property type="entry name" value="Acetyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01543">
        <property type="entry name" value="ANATRNSFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017314286.1"/>
        </source>
    </evidence>
    <sequence length="314" mass="36283" checksum="94110940C454732A" modified="2017-12-20" version="1">MVVCEGFFSRRHAGLVIQLYFATVMDLQEYFRRIGFGGTYEKPDLATLRTVHELHVMSVPFENLSIHCGERNTMDLQIIYEKIVRNRRGGWCCENNLLFSWVLREMGYKYTTLGSRVFKTFKNDFSPMDSHLINMVEIEGKPYIADVSFGLSYQIWYPLEIIAGKDQEQPAGIFRLLNDGEKWMLQKTGRKPLVQNEAFANSSLINKRLTTMLYSFVLTPREIDHFLNTSEYLQTSPESLFTQKSICSLQTPTGFRALIGWTFSEVTFNPEEDTDMYEMSALADSEIETILKEKFNIVLVNKLTPINSKAAYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QC34</accession>
    <name>A0A2D0QC34_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Gamma-crystallin M2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC108259744</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017314955.1">
        <property type="nucleotide sequence ID" value="XM_017459466.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QC34"/>
    <dbReference type="GeneID" id="108259744"/>
    <dbReference type="KEGG" id="ipu:108259744"/>
    <dbReference type="OMA" id="YADCMRM"/>
    <dbReference type="OrthoDB" id="8407241at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000001">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000003">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF62">
        <property type="entry name" value="CRYGM2B PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="2"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="41"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="133"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017314955.1"/>
        </source>
    </evidence>
    <sequence length="180" mass="22139" checksum="12A4D7CD9D3561DF" modified="2017-12-20" version="1">MGKIIFYEDRNFGGRSYECSSDCTDMSPYLNRCYSCRVESGCWMVYDQNNYMGNQYFLRRGEYADYMNMWGWGTNNWIRSCRMVPMYRGAYRMRMYERENFMGQMMDVTDDCDSIMDRYHWSSGCMSCHVMDGHWLMYEYPHYKGRMWYFRPGEYRSFREMMGSSGMRFMSMRRIMDSWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QCR9</accession>
    <name>A0A2D0QCR9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Metalloprotease TIKI</fullName>
            <ecNumber evidence="13">3.4.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">TRAB domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18 19 20" type="primary">trabd2a</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18 19 20" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18 19 20">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Metalloprotease that acts as a negative regulator of the Wnt signaling pathway by mediating the cleavage of the N-terminal residues of a subset of Wnt proteins. Following cleavage, Wnt proteins become oxidized and form large disulfide-bond oligomers, leading to their inactivation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="13">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="13">Divalent metal cations. Mn(2+) or Co(2+).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cell membrane</location>
            <topology evidence="13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the TIKI family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="13"/>
    <dbReference type="RefSeq" id="XP_017316087.1">
        <property type="nucleotide sequence ID" value="XM_017460598.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047007821.1">
        <property type="nucleotide sequence ID" value="XM_047151865.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053533125.1">
        <property type="nucleotide sequence ID" value="XM_053677150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QCR9"/>
    <dbReference type="GeneID" id="108260358"/>
    <dbReference type="KEGG" id="ipu:108260358"/>
    <dbReference type="CTD" id="129293"/>
    <dbReference type="OMA" id="DRYFRHE"/>
    <dbReference type="OrthoDB" id="10040378at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14789">
        <property type="entry name" value="Tiki"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040230">
        <property type="entry name" value="TIKI1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002816">
        <property type="entry name" value="TraB/PrgY/GumN_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31120">
        <property type="entry name" value="METALLOPROTEASE TIKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31120:SF7">
        <property type="entry name" value="METALLOPROTEASE TIKI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01963">
        <property type="entry name" value="TraB_PrgY_gumN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13 18" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 13" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13 16" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013510521" description="Metalloprotease TIKI" evidence="16">
        <location>
            <begin position="25"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="488"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="351"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="407"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU369069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017316087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_047007821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_053533125.1"/>
        </source>
    </evidence>
    <sequence length="513" mass="59243" checksum="6D60E8B852936DD7" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QDN6</accession>
    <name>A0A2D0QDN6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein arhgap20b isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">arhgap20b</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
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    </organism>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="6">Blood</tissue>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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        <property type="entry name" value="ARHGAP20_PH"/>
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        <property type="entry name" value="RHO-GAP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="T-CELL ACTIVATION RHO GTPASE ACTIVATING PROTEIN-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="RHG20_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017315480.1"/>
        </source>
    </evidence>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
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    </reference>
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    <dbReference type="STRING" id="7998.ENSIPUP00000009211"/>
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    <dbReference type="KEGG" id="ipu:108260241"/>
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    <dbReference type="OMA" id="DSVVCQP"/>
    <dbReference type="OrthoDB" id="6431454at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043296">
        <property type="term" value="C:apical junction complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:cell-cell junction organization"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF26649"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017315831.1"/>
        </source>
    </evidence>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
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                <person name="Peatman E."/>
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                <person name="Qin Z."/>
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                <person name="Sonstegard T."/>
                <person name="Feng J."/>
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                <person name="Duke M.V."/>
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                <person name="Kucuktas H."/>
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                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
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                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
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        <text evidence="2">Belongs to the GARNL3 family.</text>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
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    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="GTPase-activating Rap/Ran-GAP domain-like protein 3 isoform X1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035974">
        <property type="entry name" value="Rap/Ran-GAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000331">
        <property type="entry name" value="Rap/Ran_GAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050989">
        <property type="entry name" value="Rap1_Ran_GAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711:SF62">
        <property type="entry name" value="GTPASE-ACTIVATING RAP_RAN-GAP DOMAIN-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711">
        <property type="entry name" value="RAP GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02145">
        <property type="entry name" value="Rap_GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111347">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50085">
        <property type="entry name" value="RAPGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rap-GAP" evidence="5">
        <location>
            <begin position="200"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="6">
        <location>
            <begin position="504"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="835"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="960"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="838"/>
            <end position="850"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00060925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00069072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017315940.1"/>
        </source>
    </evidence>
    <sequence length="1050" mass="117795" checksum="0590B07F003BE8C0" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QHU0</accession>
    <name>A0A2D0QHU0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Serine/threonine-protein kinase haspin</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Germ cell-specific gene 2 protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25 26" type="primary">haspin</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">SDA103</strain>
        </source>
    </reference>
    <reference evidence="25 26" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25 26">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Serine/threonine-protein kinase that phosphorylates histone H3 at 'Thr-3' (H3T3ph) during mitosis. May act through H3T3ph to both position and modulate activation of AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017317060.1">
        <property type="nucleotide sequence ID" value="XM_017461571.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017317062.1">
        <property type="nucleotide sequence ID" value="XM_017461573.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QHU0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007574"/>
    <dbReference type="GeneID" id="108260887"/>
    <dbReference type="KEGG" id="ipu:108260887"/>
    <dbReference type="CTD" id="83903"/>
    <dbReference type="OMA" id="NECAGND"/>
    <dbReference type="OrthoDB" id="21018at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072354">
        <property type="term" value="F:histone H3T3 kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901991">
        <property type="term" value="P:negative regulation of mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000401">
        <property type="entry name" value="serine/threonine-protein kinase haspin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000409">
        <property type="entry name" value="serine/threonine-protein kinase haspin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024604">
        <property type="entry name" value="GSG2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24419">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR-ASSOCIATED KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24419:SF18">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE HASPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12330">
        <property type="entry name" value="Haspin_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01331">
        <property type="entry name" value="DUF3635"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="798"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="35"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="406"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="619"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="60"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="365"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="826"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00053811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00069281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00081741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_017317060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_017317062.1"/>
        </source>
    </evidence>
    <sequence length="1112" mass="123644" checksum="5C9AC712DA2D7113" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QK27</accession>
    <name>A0A2D0QK27_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Phospholipid phosphatase 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">plpp4</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017318682.1">
        <property type="nucleotide sequence ID" value="XM_017463193.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QK27"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022926"/>
    <dbReference type="GeneID" id="108262841"/>
    <dbReference type="KEGG" id="ipu:108262841"/>
    <dbReference type="CTD" id="196051"/>
    <dbReference type="OMA" id="WFSYRRY"/>
    <dbReference type="OrthoDB" id="10030083at2759"/>
    <dbReference type="UniPathway" id="UPA00085"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03390">
        <property type="entry name" value="PAP2_containing_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.144.10:FF:000029">
        <property type="entry name" value="Phospholipid phosphatase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF90">
        <property type="entry name" value="PHOSPHOLIPID PHOSPHATASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="8">
        <location>
            <begin position="94"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="266"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017318682.1"/>
        </source>
    </evidence>
    <sequence length="286" mass="32152" checksum="3EAD644B5DE3DF29" modified="2017-12-20" version="1">MKMSPPDRRKGMRELALEIGIRVLLFGVFVFTEFLEPFERVIQPEELWLYKNPLVESDLIPKRVMFAISFLTPLAVIFVVKIIRRTDKTEIKEACLAVSLALALNGVFTNTIKLIVGRPRPDYFHRCFPDGQVNAKMLCTGEPSLVSEGRKSFPSSHSSFAFSGLGFTSFYLAGKLHCFTESGRGRSWRLCAMILPLYSALMIALSRTCDYKHHWQDAFVGGVIGLLFAYICYRQHYPPFLHTDCHLPYASLALCTTRHAPVSQEVPQATDNATSLPLEGITEGPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QK54</accession>
    <name>A0A2D0QK54_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Synapse differentiation-inducing gene protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Dispanin subfamily C member 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Transmembrane protein 90B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC108262873</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">May regulate AMPA receptor content at nascent synapses, and have a role in postsynaptic development and maturation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homodimer. Interacts with GRIA1 and GRIA2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Early endosome membrane</location>
            <topology evidence="16">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Postsynaptic density membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CD225/Dispanin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017318717.1">
        <property type="nucleotide sequence ID" value="XM_017463228.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QK54"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019219"/>
    <dbReference type="GeneID" id="108262873"/>
    <dbReference type="KEGG" id="ipu:108262873"/>
    <dbReference type="OMA" id="SWGDGMA"/>
    <dbReference type="OrthoDB" id="8440917at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060076">
        <property type="term" value="C:excitatory synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051965">
        <property type="term" value="P:positive regulation of synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097091">
        <property type="term" value="P:synaptic vesicle clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007593">
        <property type="entry name" value="CD225/Dispanin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14768:SF3">
        <property type="entry name" value="SYNAPSE DIFFERENTIATION-INDUCING GENE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14768">
        <property type="entry name" value="UPF0338 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04505">
        <property type="entry name" value="CD225"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="11 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="10" id="KW-0770">Synapse</keyword>
    <keyword evidence="6 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="202"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="246"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00037858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00038680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00040240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00041354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00042246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_017318717.1"/>
        </source>
    </evidence>
    <sequence length="278" mass="30587" checksum="BA5C0053E77731CF" modified="2017-12-20" version="1">MESGLRTAPRAEEEKAPPAQRKLSRAGGGDLGPECCKCNGQINTRSVMVETKEGRVSVYSAPQYQSQVVVSYSPEHADQDRNGHHPQLLNPSALPQALDPFYKPNLILYPEGVLKVWGEGSDCCETTFIEDMAPANSAATVTKDGLMFTDKKFLDLSMEDTKIHTLSYDVDDDDEFEELESEYSSDSESEDNFLMMPPRDHLGLSVFSMLCCFWPLGIAAFYLSHQTNKAVNKGDLHHASSSSRRALFLAVLSITIGTGIYVGVAVALIAYLSKNNHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QL15</accession>
    <name>A0A2D0QL15_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DENN domain-containing protein 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">dennd10</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DENND10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017319178.1">
        <property type="nucleotide sequence ID" value="XM_017463689.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QL15"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027266"/>
    <dbReference type="GeneID" id="108263166"/>
    <dbReference type="KEGG" id="ipu:108263166"/>
    <dbReference type="CTD" id="404636"/>
    <dbReference type="OMA" id="HKDIAMF"/>
    <dbReference type="OrthoDB" id="66409at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000641">
        <property type="term" value="P:regulation of early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042431">
        <property type="entry name" value="FAM45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28544:SF1">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28544">
        <property type="entry name" value="PROTEIN FAM45A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08616">
        <property type="entry name" value="SPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="5">
        <location>
            <begin position="1"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017319178.1"/>
        </source>
    </evidence>
    <sequence length="351" mass="39991" checksum="98DA7EBFDFA45300" modified="2017-12-20" version="1">MAVSETQLMLSVGLIGKDVNADTLWVWCFPAVSAELRELLMRKCCLSRDSPELHAYVFGQYRRSWYYLTTTDVQEPTALTKVTHFSIVITAKDFNPEKYAAFSRVLCRMYMKHGSPVKMMEGYIAVLTKGICQSDENGSFLIKDYDVRKAYLAGSIKDVVSQFGMETIILYTALMLKKRIVIYHPRVEALLEFTRVLPALTWHRKDWSILHPYVHLEDRELDNLRSCTGYVAGFVDPDVINRSDLFDVFVNLPDNEITIARHAKEAMTMGKLHKEIGHLIVQSAEDADRTDAQVIKDISVKTKEILSNLMSLAEDGDDAKLTLDTLKRRHLPPATENFLFHLAAAEQLLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QMY9</accession>
    <name>A0A2D0QMY9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ras-related protein Rab-1A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rab1ab</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017319669.1">
        <property type="nucleotide sequence ID" value="XM_017464180.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QMY9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030373"/>
    <dbReference type="GeneID" id="108263420"/>
    <dbReference type="KEGG" id="ipu:108263420"/>
    <dbReference type="CTD" id="368883"/>
    <dbReference type="OMA" id="TQMAKDF"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01869">
        <property type="entry name" value="Rab1_Ypt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000069">
        <property type="entry name" value="Ras GTP-binding protein YPT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057289">
        <property type="entry name" value="Rab1/Ypt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="173"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="180"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017319669.1"/>
        </source>
    </evidence>
    <sequence length="202" mass="22410" checksum="31708E5031C5E73D" modified="2017-12-20" version="1">MNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKLLVGNKCDLTTKKVVDYTTAKEFADSLGIPFLETSAKNATNVEQAFMTMAAEIKKRMGPGATTGGTEKSNVKIQSTPVKPAPGGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QPC6</accession>
    <name>A0A2D0QPC6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">V-set and transmembrane domain-containing protein 4a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">vstm4a</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017319581.1">
        <property type="nucleotide sequence ID" value="XM_017464092.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QPC6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000316"/>
    <dbReference type="GeneID" id="108263377"/>
    <dbReference type="KEGG" id="ipu:108263377"/>
    <dbReference type="CTD" id="553443"/>
    <dbReference type="OrthoDB" id="8885867at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001953">
        <property type="entry name" value="V-set and transmembrane domain containing 4b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051102">
        <property type="entry name" value="IgSF_V-set/TM_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207">
        <property type="entry name" value="V-SET AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207:SF8">
        <property type="entry name" value="V-SET AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012880970" evidence="6">
        <location>
            <begin position="23"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="23"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="216"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="216"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="230"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017319581.1"/>
        </source>
    </evidence>
    <sequence length="329" mass="37307" checksum="CE2E43EFCBE3C124" modified="2017-12-20" version="1">MKLSSVVMVLLTRALIGELAEALNVTVTPGPVTMCLEGENITVSCLVSQKKRSSSVLVLRWLYFPKPEDERLVVKMNFKKAKYYGNYSLSFSQTKFHLWEEVEGQIYSLLILNVSREDRGNYSCKVQEIRKHRNKWRASANGTGTMELQVHYIGIPESTDNIWRWFQDVYLCAVLICSVGLISTVLFIVKITCQYMQHKQRRKARFYLMKCPESSSGETVTSVASSSPGMHRKEKRHKAPPKDIAAQPPVVPVKAPVPNKPHRTKLLKVQPRKAHMPRVADDSLTYAELELVKRVPEAKRNSSGTAQLETNAHCTGTVYAQILFVEKQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QUQ4</accession>
    <name>A0A2D0QUQ4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Carnitine O-palmitoyltransferase 1, muscle isoform</fullName>
            <ecNumber evidence="4">2.3.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Carnitine O-palmitoyltransferase I, muscle isoform</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Carnitine palmitoyltransferase 1B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26 27 28 29" type="primary">cpt1b</name>
    </gene>
    <organism evidence="25 27">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">SDA103</strain>
        </source>
    </reference>
    <reference evidence="26 27 28" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26 27 28">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(R)-carnitine + hexadecanoyl-CoA = O-hexadecanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:12661"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:17490"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="EC" id="2.3.1.21"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:12662"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 21">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.21" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017321469.1">
        <property type="nucleotide sequence ID" value="XM_017465980.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017321471.1">
        <property type="nucleotide sequence ID" value="XM_017465982.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534091.1">
        <property type="nucleotide sequence ID" value="XM_053678116.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534092.1">
        <property type="nucleotide sequence ID" value="XM_053678117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QUQ4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000011207"/>
    <dbReference type="GeneID" id="108264444"/>
    <dbReference type="KEGG" id="ipu:108264444"/>
    <dbReference type="CTD" id="1375"/>
    <dbReference type="OMA" id="PERCGPY"/>
    <dbReference type="OrthoDB" id="240216at2759"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004095">
        <property type="term" value="F:carnitine O-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009437">
        <property type="term" value="P:carnitine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015909">
        <property type="term" value="P:long-chain fatty acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.70:FF:000001">
        <property type="entry name" value="Carnitine O-palmitoyltransferase 1, liver isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000042">
        <property type="entry name" value="Carnitine Palmitoyl Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032476">
        <property type="entry name" value="CPT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF69">
        <property type="entry name" value="CARNITINE O-PALMITOYLTRANSFERASE 1, MUSCLE ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16484">
        <property type="entry name" value="CPT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00439">
        <property type="entry name" value="ACYLTRANSF_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00440">
        <property type="entry name" value="ACYLTRANSF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Carnitine O-palmitoyltransferase N-terminal" evidence="24">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="23">
        <location>
            <begin position="174"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="20">
        <location>
            <position position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00043926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU003801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF16484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_017321469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_017321471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_053534091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_053534092.1"/>
        </source>
    </evidence>
    <sequence length="770" mass="88313" checksum="6EABD783942A1A0C" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QWZ4</accession>
    <name>A0A2D0QWZ4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10">MPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">mpnd</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Probable protease. Acts as a sensor of N(6)-methyladenosine methylation on DNA (m6A): recognizes and binds m6A DNA, leading to its degradation. Binds only double strand DNA (dsDNA) in a sequence-independent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M67 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017322928.1">
        <property type="nucleotide sequence ID" value="XM_017467439.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QWZ4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000023242"/>
    <dbReference type="GeneID" id="108265286"/>
    <dbReference type="KEGG" id="ipu:108265286"/>
    <dbReference type="CTD" id="84954"/>
    <dbReference type="OMA" id="VEMVYVQ"/>
    <dbReference type="OrthoDB" id="167806at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08067">
        <property type="entry name" value="MPN_2A_DUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000053">
        <property type="entry name" value="MPN domain-containing protein CG4751"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050242">
        <property type="entry name" value="JAMM_MPN+_peptidase_M67A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040843">
        <property type="entry name" value="RAMA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10410">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18755">
        <property type="entry name" value="RAMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="10">
        <location>
            <begin position="229"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="144"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="148"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="162"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00093358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017322928.1"/>
        </source>
    </evidence>
    <sequence length="458" mass="51354" checksum="2A909E65EC3BD62D" modified="2017-12-20" version="1">MGSEPPSSPQLVDEGVDEDDEELSGGEEVELRVSSGRGSVLTRRGITLRVLLKDGLVEPGDAVLSIHYLGKNFVGDLLTDGKIRWVETGQVFNSPSAWATHCKRLVNPAKKSGCGWASVRYRGQKLAQYKTSWLRKYQPSADMSLASEGEDDEMLDDEEDEGKTTIPDERNRKSRPELHDVSVVHRRGDRERIPVRYCTLGTRDIARDPHTLVELSAFSAINRFQPFNVAVSSNVLLLMDFHCHLTTSEVVGYLGGRWDTTTQLLTVLRAFPCRTRLADRDAAPAVEEEICQNLFMRGLSLVGWYHSHPRGPALPSLQDIDSQMDHQLRLQGSSNGFQPCLGIICGPYYHGNQGVASTITPFWVVPPPEQRPNDYGIPVAVEVTYVQDNFLTTDVLNEMMMLVEFYRAAPDLVNFNQMWCSDTSILEKMKASLSGHAPKDQAYSQILEHVYNQLRNMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QZ32</accession>
    <name>A0A2D0QZ32_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BAG family molecular chaperone regulator 4 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">bag4</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017323016.1">
        <property type="nucleotide sequence ID" value="XM_017467527.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QZ32"/>
    <dbReference type="GeneID" id="108265327"/>
    <dbReference type="KEGG" id="ipu:108265327"/>
    <dbReference type="CTD" id="9530"/>
    <dbReference type="OrthoDB" id="8614100at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.120">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039773">
        <property type="entry name" value="BAG_chaperone_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036533">
        <property type="entry name" value="BAG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329:SF10">
        <property type="entry name" value="BAG FAMILY MOLECULAR CHAPERONE REGULATOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329">
        <property type="entry name" value="BCL2-ASSOCIATED ATHANOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00264">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51035">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAG" evidence="3">
        <location>
            <begin position="273"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="66"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="106"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="185"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="256"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017323016.1"/>
        </source>
    </evidence>
    <sequence length="351" mass="38482" checksum="F2BCBFB32404ED0E" modified="2017-12-20" version="1">MHRENQSDPKSDGYTSTSNGHSWNNAMQDSTPYCGYPPNYWYPPPAGPYTNAYPSGTEINGQAAYNPQAMPAYPNGVYNPGQYPPNVLHPSNPFYCSDQIPPRQPQYHSQERTTASSPQPPYPVPHCQGAPGFPPSSYSPYGDGCPPNAPYPAQQPVPSRSQPEPWPHSAGYGPQAHYPNHTRPPHPPPWHGPAPPPYDHIKHKEPPYPGHLSNRNRPGTPNSNPAPNPAPNKQVEFSAPPQIYNKTSSGGANQPNPDPAPAAPQPPSSENSGLARVQEVLARVHLLQEDVDEFVGKKTDKSYRCLEELLTKELLVLDSVETNGQDGVRSARKEAVQKIQAILDRLENKAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R091</accession>
    <name>A0A2D0R091_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC108265832</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108265832</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017324062.1">
        <property type="nucleotide sequence ID" value="XM_017468573.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R091"/>
    <dbReference type="GeneID" id="108265832"/>
    <dbReference type="KEGG" id="ipu:108265832"/>
    <dbReference type="OrthoDB" id="8946945at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017324062.1"/>
        </source>
    </evidence>
    <sequence length="286" mass="32196" checksum="F6620E28045F5AB5" modified="2017-12-20" version="1">MGKISCRRRSKRRRSNSIQRENDQQGVISDNVKQESEISEHSFSEPRHNENESDVFSNSLSLNTSAVDGSHVFFPVFSHSNVGQVTINSTYAINNKSPDTRERNPESQAQDGSSDVDASATTILRTPKKSKKFRRSRRSAARRSLTHFCGPRDIVGEITGAHVTSEYHCSDCRFKIPSWKPLCFNVKCPGCKMSWKCDKIQCTCKAKITLELPSGQTQRVTLDDSLMRSIVRFKTQGYCKTERIETKILKIGSVRVVYNDGVLRSVSKISSSSMSLRDIPDAFPSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R0E0</accession>
    <name>A0A2D0R0E0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Nuclear receptor subfamily 2 group C member 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">nr2c2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017323491.1">
        <property type="nucleotide sequence ID" value="XM_017468002.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R0E0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033386"/>
    <dbReference type="GeneID" id="108265548"/>
    <dbReference type="KEGG" id="ipu:108265548"/>
    <dbReference type="CTD" id="7182"/>
    <dbReference type="OrthoDB" id="10024684at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06967">
        <property type="entry name" value="NR_DBD_TR2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06952">
        <property type="entry name" value="NR_LBD_TR2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000012">
        <property type="entry name" value="Nuclear receptor subfamily 2 group C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000015">
        <property type="entry name" value="Nuclear receptor subfamily 2, group C, member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048245">
        <property type="entry name" value="NR2C1/2-like_DBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048246">
        <property type="entry name" value="NR2C1/2-like_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050274">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24083">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9 11 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="12">
        <location>
            <begin position="145"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="13">
        <location>
            <begin position="371"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017323491.1"/>
        </source>
    </evidence>
    <sequence length="626" mass="68757" checksum="C429809997AE3E7C" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R0Q6</accession>
    <name>A0A2D0R0Q6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Actin nucleation-promoting factor WAS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">wasa</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017323611.1">
        <property type="nucleotide sequence ID" value="XM_017468122.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R0Q6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000004896"/>
    <dbReference type="GeneID" id="108265595"/>
    <dbReference type="KEGG" id="ipu:108265595"/>
    <dbReference type="CTD" id="571256"/>
    <dbReference type="OMA" id="PPNDECY"/>
    <dbReference type="OrthoDB" id="8963340at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01205">
        <property type="entry name" value="EVH1_WASP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21762">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000130">
        <property type="entry name" value="neural Wiskott-Aldrich syndrome protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000017">
        <property type="entry name" value="Wiskott-Aldrich syndrome (eczema-thrombocytopenia) b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011026">
        <property type="entry name" value="WAS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033927">
        <property type="entry name" value="WASPfam_EVH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000697">
        <property type="entry name" value="WH1/EVH1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202:SF36">
        <property type="entry name" value="ACTIN NUCLEATION-PROMOTING FACTOR WASL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202">
        <property type="entry name" value="SPROUTY-RELATED, EVH1 DOMAIN-CONTAINING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00568">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00461">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47912">
        <property type="entry name" value="Wiscott-Aldrich syndrome protein, WASP, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50229">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="WH1" evidence="10">
        <location>
            <begin position="30"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="CRIB" evidence="9">
        <location>
            <begin position="213"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="WH2" evidence="11">
        <location>
            <begin position="565"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="140"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="273"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="613"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="320"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="397"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="477"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="498"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="533"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="620"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017323611.1"/>
        </source>
    </evidence>
    <sequence length="635" mass="67653" checksum="789DBE3EF2038048" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R141</accession>
    <name>A0A2D0R141_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lens epithelial cell protein LEP503</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">lenep</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SDA103</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017324254.1">
        <property type="nucleotide sequence ID" value="XM_017468765.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A2D0R141"/>
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    <dbReference type="KEGG" id="ipu:108265916"/>
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    <dbReference type="OrthoDB" id="8727558at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029194">
        <property type="entry name" value="LEP503"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16968">
        <property type="entry name" value="LENS EPITHELIAL CELL PROTEIN LEP503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16968:SF2">
        <property type="entry name" value="LENS EPITHELIAL CELL PROTEIN LEP503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15221">
        <property type="entry name" value="LEP503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_017324254.1"/>
        </source>
    </evidence>
    <sequence length="63" mass="6952" checksum="71E17C301A683697" modified="2017-12-20" version="1">MHPQRPLAQAVPASLGQNLRDLAYGFGPGKKLLGGNISYGVIQSLKECLYFLLCCWCIKEILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R3H3</accession>
    <name>A0A2D0R3H3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">FERM, ARHGEF and pleckstrin domain-containing protein 1</fullName>
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        <alternativeName>
            <fullName evidence="17">FERM, RhoGEF and pleckstrin domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23 24" type="primary">LOC108266411</name>
    </gene>
    <organism evidence="22 24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23 24" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23 24">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Filopodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Synapse</location>
            <location evidence="15">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017325146.1">
        <property type="nucleotide sequence ID" value="XM_017469657.3"/>
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        <property type="nucleotide sequence ID" value="XM_017469658.3"/>
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        <property type="component" value="Chromosome 6"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="FERM_B-lobe"/>
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    <dbReference type="CDD" id="cd13193">
        <property type="entry name" value="FERM_C_FARP1-like"/>
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        <property type="entry name" value="FERM_F1_FARP1"/>
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    <dbReference type="CDD" id="cd01220">
        <property type="entry name" value="PH1_FARP1-like"/>
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        <property type="entry name" value="PH2_FARP1-like"/>
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        <property type="entry name" value="RhoGEF"/>
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        <property type="entry name" value="Band 4.1-like protein 5 isoform 1"/>
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        <property type="entry name" value="FERM, RhoGEF and pleckstrin domain-containing protein"/>
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        <property type="entry name" value="FERM, RhoGEF and pleckstrin domain-containing protein 1"/>
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    </dbReference>
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        <property type="entry name" value="FERM, RhoGEF and pleckstrin domain-containing protein 1"/>
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        <property type="entry name" value="FERM, RhoGEF and pleckstrin domain-containing protein 1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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        <property type="entry name" value="Band_41_domain"/>
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    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
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    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
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    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
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    <dbReference type="InterPro" id="IPR014847">
        <property type="entry name" value="FA"/>
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    <dbReference type="InterPro" id="IPR041788">
        <property type="entry name" value="FARP1/FARP2/FRMD7_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
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    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
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    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051835">
        <property type="entry name" value="RAC1-GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45858">
        <property type="entry name" value="FERM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45858:SF2">
        <property type="entry name" value="FERM, ARHGEF AND PLECKSTRIN DOMAIN-CONTAINING PROTEIN 1"/>
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        <property type="entry name" value="FA"/>
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    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
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        <property type="entry name" value="FERM_N"/>
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        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
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    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
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    <dbReference type="PRINTS" id="PR00661">
        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01195">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
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    </dbReference>
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        <property type="entry name" value="RhoGEF"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
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    </dbReference>
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        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0770">Synapse</keyword>
    <keyword evidence="9" id="KW-0771">Synaptosome</keyword>
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        <location>
            <begin position="40"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="20">
        <location>
            <begin position="503"/>
            <end position="694"/>
        </location>
    </feature>
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        <location>
            <begin position="723"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="19">
        <location>
            <begin position="898"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="366"/>
            <end position="390"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="409"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="457"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="843"/>
            <end position="871"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="369"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="464"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="476"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004486"/>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00042170"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50003"/>
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            <dbReference type="PROSITE" id="PS50010"/>
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    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
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    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017325146.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_017325147.1"/>
        </source>
    </evidence>
    <sequence length="1009" mass="115241" checksum="7FCE7637C88B98B6" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R609</accession>
    <name>A0A2D0R609_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Gamma-crystallin M2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC108266801</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017326033.2">
        <property type="nucleotide sequence ID" value="XM_017470544.3"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000001">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000003">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF122">
        <property type="entry name" value="GAMMA-CRYSTALLIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="2"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="41"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="130"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017326033.2"/>
        </source>
    </evidence>
    <sequence length="174" mass="21089" checksum="C75FD9557C4561B7" modified="2023-06-28" version="2">MGKIIFYEDTNFQGRSYECSSDCTDLHSYFSHCNSIRVESGCFMIYERPNYIGQQYFMKKGDYSDFHSWKGVSSSIRSCRIIPPYRGIYRLRLYEKADFNGNMMEFMDDCSSVSDRFHHRHVYSCKVMNGYWIFYEYPNYRGRQYFLKPGEYRRYRDWRATCSIVGSFRRITDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R918</accession>
    <name>A0A2D0R918_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Uncharacterized protein LOC108267488</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">LOC108267488</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3 4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017327074.1">
        <property type="nucleotide sequence ID" value="XM_017471585.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053537132.1">
        <property type="nucleotide sequence ID" value="XM_053681157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R918"/>
    <dbReference type="GeneID" id="108267488"/>
    <dbReference type="KEGG" id="ipu:108267488"/>
    <dbReference type="OrthoDB" id="9978460at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017327074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053537132.1"/>
        </source>
    </evidence>
    <sequence length="165" mass="18967" checksum="9D99C9B97918076A" modified="2017-12-20" version="1">MEAAETPSSHVLSTRGDEKTRDGEVVCKIVFEEYLSEWVKIATTRSGHLKGKESLDEAIRRPERFAARYGVSGSENDELVEEPRKIYKGTMNGPRRSKKHKSRQTRVEQMRRIYLLCRTPDTAAPPVPALKDSVHDAERRECWESEVDKLVAWTHSLSFEEPDRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RDD8</accession>
    <name>A0A2D0RDD8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="19 20">Platelet endothelial aggregation receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19 20" type="primary">pear1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19 20" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19 20">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the MEGF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017328133.1">
        <property type="nucleotide sequence ID" value="XM_017472644.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053537828.1">
        <property type="nucleotide sequence ID" value="XM_053681853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RDD8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009229"/>
    <dbReference type="GeneID" id="108268012"/>
    <dbReference type="KEGG" id="ipu:108268012"/>
    <dbReference type="CTD" id="375033"/>
    <dbReference type="GeneTree" id="ENSGT00940000155333"/>
    <dbReference type="OrthoDB" id="10268124at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.300.10:FF:000002">
        <property type="entry name" value="Multiple epidermal growth factor-like domains 10"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000114">
        <property type="entry name" value="Multiple epidermal growth factor-like domains protein 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.300.10:FF:000041">
        <property type="entry name" value="Tyrosine protein kinase receptor tie-1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057138">
        <property type="entry name" value="EGF_PEAR1L-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011489">
        <property type="entry name" value="EMI_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052485">
        <property type="entry name" value="MEGF_diff_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24052">
        <property type="entry name" value="DELTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24052:SF12">
        <property type="entry name" value="PLATELET ENDOTHELIAL AGGREGATION RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23301">
        <property type="entry name" value="EGF_PEAR1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51041">
        <property type="entry name" value="EMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 12" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="19 20" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013510607" evidence="15">
        <location>
            <begin position="23"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="753"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="domain" description="EMI" evidence="17">
        <location>
            <begin position="27"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="141"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="229"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="271"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="398"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="484"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="570"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="657"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="982"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="248"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="291"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="423"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="509"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="595"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="682"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017328133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_053537828.1"/>
        </source>
    </evidence>
    <sequence length="1020" mass="111380" checksum="D3580C13691AF404" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RI30</accession>
    <name>A0A2D0RI30_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9 10">Highly divergent homeobox isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9 10" type="primary">hdx</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8 9 10" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8 9 10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017330187.1">
        <property type="nucleotide sequence ID" value="XM_017474698.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017330188.1">
        <property type="nucleotide sequence ID" value="XM_017474699.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017330189.1">
        <property type="nucleotide sequence ID" value="XM_017474700.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RI30"/>
    <dbReference type="GeneID" id="108269119"/>
    <dbReference type="KEGG" id="ipu:108269119"/>
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    <dbReference type="OMA" id="QPCKIEP"/>
    <dbReference type="OrthoDB" id="10055960at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF80">
        <property type="entry name" value="HIGHLY DIVERGENT HOMEOBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="6">
        <location>
            <begin position="10"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="6">
        <location>
            <begin position="444"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="12"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="446"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="115"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="246"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="514"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="666"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="127"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="246"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="296"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="685"/>
            <end position="700"/>
        </location>
    </feature>
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        <location>
            <begin position="702"/>
            <end position="711"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017330187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017330188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017330189.1"/>
        </source>
    </evidence>
    <sequence length="711" mass="78172" checksum="ED3D3E40D141460E" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RMJ6</accession>
    <name>A0A2D0RMJ6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Kelch domain-containing protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">klhdc2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5 6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017331371.1">
        <property type="nucleotide sequence ID" value="XM_017475882.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017331372.1">
        <property type="nucleotide sequence ID" value="XM_017475883.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RMJ6"/>
    <dbReference type="GeneID" id="108269821"/>
    <dbReference type="KEGG" id="ipu:108269821"/>
    <dbReference type="CTD" id="23588"/>
    <dbReference type="GeneTree" id="ENSGT00940000157150"/>
    <dbReference type="OrthoDB" id="10251809at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46228">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46228:SF3">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017331371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017331372.1"/>
        </source>
    </evidence>
    <sequence length="443" mass="50702" checksum="C0BF938F7D93A760" modified="2017-12-20" version="1">MADQDGDGNLQDVREELDESEDEEEGDEERNVGWHEQEELDWSEDDGSDLESISDTPAERSGHIAVVDRNCIYVWGGYKNAEAPGFFDLYLPRHEIWIYNMETGRWRRQMSGGDLPNPMSGSCGVCVEGILYLFGGHHARGNTNLVYRLPLRASYLHWERMKDLKGLAPTCKDKLGCWVYMNRLVYFGGYGYEAEADHRGTFELDENSLMGNHQGRGWNNHVHILNLETSSWSQPITKVYKSQSLIHLHATQGNAPTPRAAHACATMGNRGYVFGGRYMDHRLNDLYYINMDTWEWTEMSVPQHGPVGRSWHSFIPVSSDHIFLFGGFTTNRETLSDAWLYCETTNEWKQLKHNHKKSPRLWHTACLGLDGEVFVFGGCANNLLSNQQASHSNELLIFTVQPKSLLRCCTEVVVQHRHQLESMFDCLPKHLLHSLQQRFSSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RNM8</accession>
    <name>A0A2D0RNM8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Nucleic acid dioxygenase ALKBH1</fullName>
            <ecNumber evidence="24">1.14.11.33</ecNumber>
            <ecNumber evidence="23">1.14.11.51</ecNumber>
            <ecNumber evidence="2">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="28">Alkylated DNA repair protein alkB homolog 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="30">Alpha-ketoglutarate-dependent dioxygenase ABH1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">DNA 6mA demethylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">DNA N6-methyl adenine demethylase ALKBH1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="32">DNA lyase ABH1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">DNA oxidative demethylase ALKBH1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="31">mRNA N(3)-methylcytidine demethylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="37" type="primary">alkbh1</name>
    </gene>
    <organism evidence="36 37">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="36" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="36">SDA103</strain>
        </source>
    </reference>
    <reference evidence="37" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="37">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>5-methylcytidine(34) in mitochondrial tRNA(Met) + 2 2-oxoglutarate + 2 O2 = 5-formylcytidine(34) in mitochondrial tRNA(Met) + 2 succinate + 2 CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:54144"/>
            <dbReference type="Rhea" id="RHEA-COMP:13808"/>
            <dbReference type="Rhea" id="RHEA-COMP:13809"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:74483"/>
            <dbReference type="ChEBI" id="CHEBI:138075"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>N(1)-methyladenosine(58) in tRNA + 2-oxoglutarate + O2 = adenosine(58) in tRNA + formaldehyde + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:79019"/>
            <dbReference type="Rhea" id="RHEA-COMP:10365"/>
            <dbReference type="Rhea" id="RHEA-COMP:10366"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74491"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a methylated nucleobase within DNA + 2-oxoglutarate + O2 = a nucleobase within DNA + formaldehyde + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:30299"/>
            <dbReference type="Rhea" id="RHEA-COMP:12192"/>
            <dbReference type="Rhea" id="RHEA-COMP:12193"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:32875"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="1.14.11.33"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>an N(1)-methyladenosine in tRNA + 2-oxoglutarate + O2 = an adenosine in tRNA + formaldehyde + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54576"/>
            <dbReference type="Rhea" id="RHEA-COMP:10242"/>
            <dbReference type="Rhea" id="RHEA-COMP:12312"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74491"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>an N(3)-methylcytidine in mRNA + 2-oxoglutarate + O2 = a cytidine in mRNA + formaldehyde + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:60920"/>
            <dbReference type="Rhea" id="RHEA-COMP:15145"/>
            <dbReference type="Rhea" id="RHEA-COMP:15713"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:74894"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:60921"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>an N(6)-methyl-2'-deoxyadenosine in DNA + 2-oxoglutarate + O2 = a 2'-deoxyadenosine in DNA + formaldehyde + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:49524"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="1.14.11.51"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="33">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="33">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="22">Monomer. Interacts with DNAJB6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.14.11.33" evidence="24"/>
    <dbReference type="EC" id="1.14.11.51" evidence="23"/>
    <dbReference type="EC" id="4.2.99.18" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017332114.1">
        <property type="nucleotide sequence ID" value="XM_017476625.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RNM8"/>
    <dbReference type="SMR" id="A0A2D0RNM8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000014964"/>
    <dbReference type="GeneID" id="108270197"/>
    <dbReference type="KEGG" id="ipu:108270197"/>
    <dbReference type="CTD" id="8846"/>
    <dbReference type="OMA" id="YKRRDPP"/>
    <dbReference type="OrthoDB" id="6614653at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035516">
        <property type="term" value="F:broad specificity oxidative DNA demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141131">
        <property type="term" value="F:DNA N6-methyladenine demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035515">
        <property type="term" value="F:oxidative RNA demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035513">
        <property type="term" value="P:oxidative RNA demethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141137">
        <property type="term" value="P:positive regulation of gene expression, epigenetic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990983">
        <property type="term" value="P:regulation of translational initiation by tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042245">
        <property type="term" value="P:RNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.590:FF:000006">
        <property type="entry name" value="AlkB homolog 1, histone H2A dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004574">
        <property type="entry name" value="Alkb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16557">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16557:SF2">
        <property type="entry name" value="NUCLEIC ACID DIOXYGENASE ALKBH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 37" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9 33" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 33" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="36" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0692">RNA repair</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="35">
        <location>
            <begin position="197"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="338"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00050564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00052138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00052237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00052866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00063554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00066586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00066725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00071276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00076476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00076973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00077991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00079723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00080338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00080514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00081604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="PIRSR" id="PIRSR604574-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="RefSeq" id="XP_017332114.1"/>
        </source>
    </evidence>
    <sequence length="374" mass="41886" checksum="3C5FEDD0BC5D028F" modified="2017-12-20" version="1">MAAKMAASMVEQGEDAFRKLFKFYKRRNPPPDFSEVIDFSKKLGNETVYTAELNHGSVSDEAAFRAGLQPVKEWRAFGLQGYPGFIFISNPFLPGSQRYWVKQCLKTYTQKPNVCNLDMHMSPAETENIWEKSVGSIRKKGQREPRTLLEKLRWVTLGYHYNWDTKNYSPDHCTPFPEDLHSLSYKIAAACGFPSFKAEAGILNYYRSDSSLGIHVDESELDHTPPLVSFSFGQTAVFLLGGTKRQDAPTAMFMRSGDIMVMSGSSRLLYHAVPCIVPAHAGHPLPSCLDQRVGKDEPDADDVRTVCEKDWEVCSAYLKTSRINMTVRQVLGPGQTFPVTHSHAVPATEGSASGYEEETQTAKKRKSESGYDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RNS2</accession>
    <name>A0A2D0RNS2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Nesprin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Enaptin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">KASH domain-containing protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Myocyte nuclear envelope protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Nuclear envelope spectrin repeat protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Synaptic nuclear envelope protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="primary">syne1b</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30">SDA103</strain>
        </source>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="31">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
            <location evidence="1">Sarcomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus outer membrane</location>
            <topology evidence="3">Single-pass type IV membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the nesprin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017331610.1">
        <property type="nucleotide sequence ID" value="XM_017476121.3"/>
    </dbReference>
    <dbReference type="GeneID" id="108269949"/>
    <dbReference type="KEGG" id="ipu:108269949"/>
    <dbReference type="CTD" id="567576"/>
    <dbReference type="OrthoDB" id="18740at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005640">
        <property type="term" value="C:nuclear outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030017">
        <property type="term" value="C:sarcomere"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21241">
        <property type="entry name" value="CH_SYNE1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21243">
        <property type="entry name" value="CH_SYNE1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000041">
        <property type="entry name" value="Nesprin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000073">
        <property type="entry name" value="Nesprin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000190">
        <property type="entry name" value="Nesprin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000033">
        <property type="entry name" value="nesprin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000037">
        <property type="entry name" value="nesprin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000139">
        <property type="entry name" value="nesprin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000119">
        <property type="entry name" value="nesprin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000137">
        <property type="entry name" value="nesprin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000112">
        <property type="entry name" value="nesprin-1 isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000177">
        <property type="entry name" value="nesprin-1 isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000233">
        <property type="entry name" value="nesprin-1 isoform X9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000126">
        <property type="entry name" value="Spectrin repeat containing, nuclear envelope 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000231">
        <property type="entry name" value="Spectrin repeat containing, nuclear envelope 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000386">
        <property type="entry name" value="Spectrin repeat containing, nuclear envelope 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000480">
        <property type="entry name" value="Spectrin repeat containing, nuclear envelope 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000478">
        <property type="entry name" value="Spectrin repeat-containing, nuclear envelope 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="33"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047290">
        <property type="entry name" value="CH_SYNE1_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047291">
        <property type="entry name" value="CH_SYNE1_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012315">
        <property type="entry name" value="KASH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057057">
        <property type="entry name" value="Spectrin_SYNE1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057932">
        <property type="entry name" value="Spectrin_SYNE1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056887">
        <property type="entry name" value="SYNE1/2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14514:SF3">
        <property type="entry name" value="NESPRIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14514">
        <property type="entry name" value="PKA ANCHORING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10541">
        <property type="entry name" value="KASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25034">
        <property type="entry name" value="Spectrin_SYNE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25803">
        <property type="entry name" value="Spectrin_SYNE1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25035">
        <property type="entry name" value="SYNE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01249">
        <property type="entry name" value="KASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="44"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51049">
        <property type="entry name" value="KASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="12 25" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0221">Differentiation</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13 24 27" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="7 24 27" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 27" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="24">
        <location>
            <begin position="1"/>
            <end position="8732"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="8730"/>
            <end position="8751"/>
        </location>
    </feature>
    <feature type="topological domain" description="Perinuclear space" evidence="24">
        <location>
            <begin position="8754"/>
            <end position="8783"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="28">
        <location>
            <begin position="31"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="28">
        <location>
            <begin position="181"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="KASH" evidence="29">
        <location>
            <begin position="8724"/>
            <end position="8783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="5839"/>
            <end position="5872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="6163"/>
            <end position="6182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="8208"/>
            <end position="8243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="8657"/>
            <end position="8714"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="1229"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="1455"/>
            <end position="1499"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="1692"/>
            <end position="1738"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="1789"/>
            <end position="1819"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="1979"/>
            <end position="2033"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="2220"/>
            <end position="2288"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="3201"/>
            <end position="3231"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="3482"/>
            <end position="3526"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="3574"/>
            <end position="3608"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="4120"/>
            <end position="4147"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="4510"/>
            <end position="4548"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="4775"/>
            <end position="4802"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="5591"/>
            <end position="5646"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="5725"/>
            <end position="5833"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="6193"/>
            <end position="6223"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="7019"/>
            <end position="7046"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="7125"/>
            <end position="7155"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="7774"/>
            <end position="7811"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="8459"/>
            <end position="8514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="6163"/>
            <end position="6178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="8211"/>
            <end position="8229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="26">
        <location>
            <begin position="8664"/>
            <end position="8676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="26">
        <location>
            <begin position="8677"/>
            <end position="8686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="8687"/>
            <end position="8699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00074127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00077183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00077499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00078319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00079240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00080638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="RefSeq" id="XP_017331610.1"/>
        </source>
    </evidence>
    <sequence length="8783" mass="1008585" checksum="AD1C4F3954C92441" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RQQ3</accession>
    <name>A0A2D0RQQ3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Fibroblast growth factor</fullName>
            <shortName evidence="9">FGF</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">fgf4</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017332837.1">
        <property type="nucleotide sequence ID" value="XM_017477348.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RQQ3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021439"/>
    <dbReference type="GeneID" id="108270541"/>
    <dbReference type="KEGG" id="ipu:108270541"/>
    <dbReference type="CTD" id="2249"/>
    <dbReference type="OMA" id="YESHTYP"/>
    <dbReference type="OrthoDB" id="5960247at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007368">
        <property type="term" value="P:determination of left/right symmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070121">
        <property type="term" value="P:Kupffer's vesicle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048339">
        <property type="term" value="P:paraxial mesoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060063">
        <property type="term" value="P:Spemann organizer formation at the embryonic shield"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd23316">
        <property type="entry name" value="beta-trefoil_FGF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000033">
        <property type="entry name" value="Fibroblast growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00263">
        <property type="entry name" value="HBGFFGF"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00442">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00247">
        <property type="entry name" value="HBGF_FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7" id="KW-0339">Growth factor</keyword>
    <keyword evidence="8" id="KW-0497">Mitogen</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013419724" description="Fibroblast growth factor" evidence="9">
        <location>
            <begin position="23"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU049442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017332837.1"/>
        </source>
    </evidence>
    <sequence length="185" mass="20887" checksum="6EEE624F23F968E7" modified="2017-12-20" version="1">MSVHSALLPLLVLGLVASSVRCAPLPMERRWETLYSRSLARIPGEKREVNRDSDYLTGIKRLRRLYCNVGIGFHLQVLPDGRITGVHNENRYSLLEISPVERGVVTLYGVRSELFVAMNSKGKLYGSVQYTDECKFRETLLANNYNAYESLAYPGMYIGLSRTGKTKKGNRVSTAMTVTHFLPRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RS28</accession>
    <name>A0A2D0RS28_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Relaxin family peptide receptor 3.2b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">rxfp3.2b</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017332976.1">
        <property type="nucleotide sequence ID" value="XM_017477487.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RS28"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000001285"/>
    <dbReference type="GeneID" id="108270668"/>
    <dbReference type="KEGG" id="ipu:108270668"/>
    <dbReference type="CTD" id="100004589"/>
    <dbReference type="OMA" id="FYNVQAY"/>
    <dbReference type="OrthoDB" id="9936726at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000413">
        <property type="entry name" value="Relaxin family peptide receptor 3.2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000248">
        <property type="entry name" value="ATII_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF937">
        <property type="entry name" value="RELAXIN-3 RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANGIOTENSINR"/>
    </dbReference>
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        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="286"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="77"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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            <dbReference type="PROSITE" id="PS50262"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017332976.1"/>
        </source>
    </evidence>
    <sequence length="397" mass="44208" checksum="564E0F10B057C847" modified="2017-12-20" version="1">MERNDTAPGGACESELECRAALDNCTGALDANLSVRCWLYLLTKEYDSGLQGDPSSLALRVTIALVYTVVCALGLVGNTLALYLLHSRHPQKQSSIDCLVMGLAFTDLQFVLTLPFWAVDTVMDFRWPFGRVMCKIVSSVTTMNMYASVFFLTSMSVARYYSITSSLKMHGHRSAVIKAKWVSVAIWAVSLLATLPHAVYSTIAQVAPDEELCLVRFPESGSWDPQQLLGLYQLQKVLLGFVIPLVVITLCYLLLLRFVMGRRILGSVSPETEQGRNKRRSKVTKSVVIVVLSFFLCWLPNQALTLWAVLIKFDMVPFSNAFYNAQAYAFPLSVCLAHTNSCLNPVLYCLIRREFRAGLKELVIRATPSYRSLAQLLPRKAKVAEAPTVLVLVQMDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RTZ9</accession>
    <name>A0A2D0RTZ9_ICTPU</name>
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        <submittedName>
            <fullName evidence="3 4 5">Inactive N-acetylated-alpha-linked acidic dipeptidase-like protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4 5 6" type="primary">LOC108271148</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3 4 5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3 4 5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017333969.1">
        <property type="nucleotide sequence ID" value="XM_017478480.3"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_017478481.3"/>
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        <property type="nucleotide sequence ID" value="XM_017478482.3"/>
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        <property type="nucleotide sequence ID" value="XM_053683146.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A2D0RTZ9"/>
    <dbReference type="GeneID" id="108271148"/>
    <dbReference type="KEGG" id="ipu:108271148"/>
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        <property type="component" value="Chromosome 10"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.40">
        <property type="entry name" value="Transferrin receptor-like, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zn peptidases"/>
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        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036757">
        <property type="entry name" value="TFR-like_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF32">
        <property type="entry name" value="INACTIVE N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Transferrin receptor-like dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017333969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017333970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017333971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_053539121.1"/>
        </source>
    </evidence>
    <sequence length="770" mass="84396" checksum="7152B253A94B9246" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RUD7</accession>
    <name>A0A2D0RUD7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Endophilin-A3a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">sh3gl3a</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the endophilin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017334109.1">
        <property type="nucleotide sequence ID" value="XM_017478620.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RUD7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034856"/>
    <dbReference type="GeneID" id="108271217"/>
    <dbReference type="KEGG" id="ipu:108271217"/>
    <dbReference type="CTD" id="571626"/>
    <dbReference type="OrthoDB" id="443981at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016191">
        <property type="term" value="P:synaptic vesicle uncoating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07615">
        <property type="entry name" value="BAR_Endophilin_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11803">
        <property type="entry name" value="SH3_Endophilin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000053">
        <property type="entry name" value="endophilin-A1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000021">
        <property type="entry name" value="Endophilin-A2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032469">
        <property type="entry name" value="Endophilin-A3_BAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035824">
        <property type="entry name" value="Endophilin_A_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF45">
        <property type="entry name" value="ENDOPHILIN-A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="BAR" evidence="14">
        <location>
            <begin position="18"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="323"/>
            <end position="382"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
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            <dbReference type="ARBA" id="ARBA00004412"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022753"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017334109.1"/>
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    </evidence>
    <sequence length="385" mass="43517" checksum="ED3338A508E90833" modified="2017-12-20" version="1">MSVAGLKKQFHKASQLLSEKISGAEGTKLDEDFMEMERKIDITNKSVFELLTKTTEYLQPNPASRAKLGMLNTMSKIRGQVKTTGYPQTEGLLGDCMLRYGRELGEDSTFGGALLDVGEALKQMGDVKDSLDINVKQNFIDPLQTLQDKDLKEIGHHLKKLEGRRLDFDYKKKRQGKIPDDEIKQAVEKFEESKELAERSMFNFLENDVEQVSQLAALIEAALEYHRQSSDILEELSGKLQSRINTASSKPKKEFKPKSIISSLETINSNQHNGVSYTSSLKSTDIQVNHSVNGKIDYISHPPPFNQAWSESPRVNSQTELHMDQPCCRSLYDFEPENEGELGFKEGDIIILTNQIDENWYEGMINGESGFFPINYVEVLVPLPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RY69</accession>
    <name>A0A2D0RY69_ICTPU</name>
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        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ddx27</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. DDX27/DRS1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
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        <property type="nucleotide sequence ID" value="XM_017479959.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A2D0RY69"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000036762"/>
    <dbReference type="GeneID" id="100304829"/>
    <dbReference type="KEGG" id="ipu:100304829"/>
    <dbReference type="CTD" id="55661"/>
    <dbReference type="OMA" id="MIDPPKQ"/>
    <dbReference type="OrthoDB" id="10259843at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
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    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17947">
        <property type="entry name" value="DEADc_DDX27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000842">
        <property type="entry name" value="ATP-dependent RNA helicase DRS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF22">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12 18" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="16">
        <location>
            <begin position="202"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="233"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="441"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="74"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="618"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="692"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="747"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="11">
        <location>
            <begin position="202"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="13"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="96"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="115"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="165"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="637"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="697"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="758"/>
            <end position="777"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017335448.1"/>
        </source>
    </evidence>
    <sequence length="777" mass="87928" checksum="33B8840BAAE79198" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RYE5</accession>
    <name>A0A2D0RYE5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Integrin beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="34 35" type="primary">itgb1a</name>
    </gene>
    <organism evidence="33 35">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="33">SDA103</strain>
        </source>
    </reference>
    <reference evidence="34 35" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="34 35">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="26">Cell membrane</location>
            <topology evidence="26">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Invadopodium membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Ruffle membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Melanosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 26">Belongs to the integrin beta chain family.</text>
    </comment>
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    </dbReference>
    <dbReference type="RefSeq" id="XP_017335196.1">
        <property type="nucleotide sequence ID" value="XM_017479707.3"/>
    </dbReference>
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    <dbReference type="STRING" id="7998.ENSIPUP00000000248"/>
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    <dbReference type="OrthoDB" id="410592at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0042470">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
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        <property type="term" value="F:C-X3-C chemokine binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:collagen binding involved in cell-matrix adhesion"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0001968">
        <property type="term" value="F:fibronectin binding"/>
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        <property type="term" value="F:laminin binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0033627">
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    </dbReference>
    <dbReference type="GO" id="GO:0016477">
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    </dbReference>
    <dbReference type="GO" id="GO:0098609">
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    <dbReference type="GO" id="GO:0007229">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="EGF_integrin_2"/>
    </dbReference>
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        <property type="entry name" value="Integrin_beta_N"/>
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        <property type="entry name" value="Integrin_bsu"/>
    </dbReference>
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        <property type="entry name" value="Integrin_bsu_cyt_dom"/>
    </dbReference>
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        <property type="entry name" value="Integrin_bsu_tail"/>
    </dbReference>
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        <property type="entry name" value="Integrin_bsu_tail_dom_sf"/>
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        <property type="entry name" value="Integrin_bsu_VWA"/>
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        <property type="entry name" value="Integrin_dom_sf"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="16 26" id="KW-0130">Cell adhesion</keyword>
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    <keyword evidence="7" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="23" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0517">Myogenesis</keyword>
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        </location>
    </feature>
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            <end position="800"/>
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    </feature>
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        </location>
    </feature>
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        <location>
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    </feature>
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    </feature>
    <feature type="domain" description="Integrin beta subunit tail" evidence="32">
        <location>
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            <end position="726"/>
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    </feature>
    <feature type="domain" description="Integrin beta subunit cytoplasmic" evidence="31">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
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    <feature type="disulfide bond" evidence="25">
        <location>
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    <feature type="disulfide bond" evidence="25">
        <location>
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    <feature type="disulfide bond" evidence="25">
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    <feature type="disulfide bond" evidence="25">
        <location>
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    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    <feature type="disulfide bond" evidence="25">
        <location>
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    <feature type="disulfide bond" evidence="25">
        <location>
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    <feature type="disulfide bond" evidence="25">
        <location>
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    <feature type="disulfide bond" evidence="25">
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    <feature type="disulfide bond" evidence="25">
        <location>
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    <feature type="disulfide bond" evidence="25">
        <location>
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    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    <feature type="disulfide bond" evidence="25">
        <location>
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    <feature type="disulfide bond" evidence="25">
        <location>
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    <evidence type="ECO:0000256" key="2">
        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR002512-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU000633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="SMART" id="SM00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="SMART" id="SM00423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="SMART" id="SM01241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="SMART" id="SM01242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="RefSeq" id="XP_017335187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="RefSeq" id="XP_017335196.1"/>
        </source>
    </evidence>
    <sequence length="800" mass="88340" checksum="05EAA36883ABEA59" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RZ09</accession>
    <name>A0A2D0RZ09_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Heat shock protein beta-7-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC108272085</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017335734.1">
        <property type="nucleotide sequence ID" value="XM_017480245.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RZ09"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027337"/>
    <dbReference type="GeneID" id="108272085"/>
    <dbReference type="KEGG" id="ipu:108272085"/>
    <dbReference type="OrthoDB" id="9925191at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46907:SF2">
        <property type="entry name" value="HEAT SHOCK PROTEIN BETA-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46907">
        <property type="entry name" value="HEAT SHOCK PROTEIN BETA-7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="SHSP" evidence="4">
        <location>
            <begin position="29"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017335734.1"/>
        </source>
    </evidence>
    <sequence length="123" mass="13786" checksum="049ACFB230348103" modified="2017-12-20" version="1">MQLDSFSYFFCLFFVYMLRQSLYFLAINIIMSSKESKGEVCAVGDMYTFTADLSAFSPEQVIVTSSNNLIHISAEKLESDGTILDRFSHKCQFPADVDPMSVTSSLGNNGALTVRAWKQKDKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RZ72</accession>
    <name>A0A2D0RZ72_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Chromobox protein homolog 3b isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">cbx3b</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6 7">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017335255.1">
        <property type="nucleotide sequence ID" value="XM_017479766.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047011879.1">
        <property type="nucleotide sequence ID" value="XM_047155923.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RZ72"/>
    <dbReference type="GeneID" id="108271872"/>
    <dbReference type="KEGG" id="ipu:108271872"/>
    <dbReference type="CTD" id="567497"/>
    <dbReference type="OrthoDB" id="433924at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00034">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000007">
        <property type="entry name" value="Chromobox protein homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017984">
        <property type="entry name" value="Chromo_dom_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008251">
        <property type="entry name" value="Chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051219">
        <property type="entry name" value="Heterochromatin_chromo-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22812">
        <property type="entry name" value="CHROMOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01393">
        <property type="entry name" value="Chromo_shadow"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00504">
        <property type="entry name" value="CHROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00300">
        <property type="entry name" value="ChSh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="4">
        <location>
            <begin position="37"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="4">
        <location>
            <begin position="151"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="118"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017335255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_047011879.1"/>
        </source>
    </evidence>
    <sequence length="210" mass="24576" checksum="4769C477978A06F4" modified="2017-12-20" version="1">MQGKPPHNLNSVIKENMRKKQNVKNRKAEETTVVQEFAVEKIIRRRVNDGKVEYYLKWKGFTDAENTWEPEDNLDCPELIEEFLRNLAVSGETERKDNLPLDPVIQPKEEQTELDANIQHRQQSEKEQTVCETGESTDHNTETTNDQSLIQEPERIIGSTDRQGELMFLIKWKDTDDVALLSAREASVRWPKMVIGFYEDKLSYHEEEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S0F3</accession>
    <name>A0A2D0S0F3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Chloride channel protein 1a isoform X1</fullName>
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        <name evidence="16" type="primary">clcn1a</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017480744.3"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Clc chloride channel"/>
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    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="14">
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Bao L."/>
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                <person name="Jiang C."/>
                <person name="Sun L."/>
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                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
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                <person name="Gao S."/>
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                <person name="Geng X."/>
                <person name="Song L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="12">SDA103</strain>
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        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="13" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="15"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="10">
        <location>
            <begin position="149"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="11">
        <location>
            <begin position="363"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="184"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="262"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017336542.1"/>
        </source>
    </evidence>
    <sequence length="554" mass="63565" checksum="41240FDD1B094E9D" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S6M7</accession>
    <name>A0A2D0S6M7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ataxin-7-like protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">SAGA-associated factor 11 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">atxn7l3b</name>
        <name evidence="10" type="synonym">ATXN7L3</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Component of the transcription regulatory histone acetylation (HAT) complex SAGA, a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates in a subcomplex that specifically deubiquitinates histone H2B. The SAGA complex is recruited to specific gene promoters by activators, where it is required for transcription.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of some SAGA transcription coactivator-HAT complexes. Within the SAGA complex, participates to a subcomplex of SAGA called the DUB module (deubiquitination module).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The C-terminal SGF11-type zinc-finger domain forms part of the 'catalytic lobe' of the SAGA deubiquitination module.</text>
    </comment>
    <comment type="domain">
        <text evidence="10">The long N-terminal helix forms part of the 'assembly lobe' of the SAGA deubiquitination module.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10 11">Belongs to the SGF11 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017338401.1">
        <property type="nucleotide sequence ID" value="XM_017482912.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S6M7"/>
    <dbReference type="GeneID" id="108273584"/>
    <dbReference type="KEGG" id="ipu:108273584"/>
    <dbReference type="CTD" id="552889"/>
    <dbReference type="OrthoDB" id="21557at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071819">
        <property type="term" value="C:DUBm complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000118">
        <property type="entry name" value="Ataxin-7-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03047">
        <property type="entry name" value="Sgf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013246">
        <property type="entry name" value="SAGA_su_Sgf11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051078">
        <property type="entry name" value="SGF11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46367">
        <property type="entry name" value="ATAXIN-7-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46367:SF1">
        <property type="entry name" value="ATAXIN-7-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08209">
        <property type="entry name" value="Sgf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0010">Activator</keyword>
    <keyword evidence="5 10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="zinc finger region" description="SGF11-type" evidence="10">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="116"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="165"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU261113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017338401.1"/>
        </source>
    </evidence>
    <sequence length="187" mass="20944" checksum="8BB29944A2A0A591" modified="2017-12-20" version="1">MKMEETALSGTDNSKMEGIAQEILSDLVEDACLGLCFEVHRAVKQGYFFLDDTDQESMKDFEIVDQPGVDIFGQVYNQWKNKECVCPNCSRSIAASRFAPHLEKCLGMGRNSSRIANRRIASGNNTNKSESDQEDNDDVNDNDWSYGAEKKAKKRKADKNPNSPRRSKSLKHKNTKDLSSSFSFAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S704</accession>
    <name>A0A2D0S704_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">5-demethoxyubiquinone hydroxylase, mitochondrial</fullName>
            <shortName evidence="8">DMQ hydroxylase</shortName>
            <ecNumber evidence="8">1.14.99.60</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Timing protein clk-1 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Ubiquinone biosynthesis monooxygenase COQ7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">coq7</name>
        <name evidence="8" type="synonym">COQ7</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the hydroxylation of 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) during ubiquinone biosynthesis. Has also a structural role in the COQ enzyme complex, stabilizing other COQ polypeptides. Involved in lifespan determination in a ubiquinone-independent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 5-methoxy-2-methyl-3-(all-trans-polyprenyl)benzene-1,4-diol + AH2 + O2 = a 3-demethylubiquinol + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:50908"/>
            <dbReference type="Rhea" id="RHEA-COMP:10859"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:84167"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="EC" id="1.14.99.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="8">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9. Interacts with ADCK4 and COQ6. Interacts with COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion inner membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the COQ7 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.60" evidence="8"/>
    <dbReference type="RefSeq" id="XP_017338010.1">
        <property type="nucleotide sequence ID" value="XM_017482521.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S704"/>
    <dbReference type="GeneID" id="108273351"/>
    <dbReference type="CTD" id="10229"/>
    <dbReference type="OrthoDB" id="275371at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008682">
        <property type="term" value="F:3-demethoxyubiquinol 3-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008340">
        <property type="term" value="P:determination of adult lifespan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000377">
        <property type="term" value="P:regulation of reactive oxygen species metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01042">
        <property type="entry name" value="DMQH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01658">
        <property type="entry name" value="COQ7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011566">
        <property type="entry name" value="Ubq_synth_Coq7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11237:SF4">
        <property type="entry name" value="5-DEMETHOXYUBIQUINONE HYDROXYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11237">
        <property type="entry name" value="COENZYME Q10 BIOSYNTHESIS PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03232">
        <property type="entry name" value="COQ7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017338010.1"/>
        </source>
    </evidence>
    <sequence length="200" mass="22549" checksum="929E37764BA2BB5C" modified="2017-12-20" version="1">MQRATNCVLGRVFYTLNVGNVPAVINRVNIPLVCRKYNTLPPPRNKEEKAMLDSMLRVDHAGEYGANRIYAGQMAVLGRTQTGPVIQHMWDQEKMHLQKFNEILGENRVRPTLMLPLWNVAGFLLGASTALLGKEGAMACTVAVEESISEHYNNQIRTLMEADPERYEALLQLIKEFRDDELEHHDTGLEHDAESDSAFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SAQ9</accession>
    <name>A0A2D0SAQ9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Ceramide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">tlcd3bb</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017339541.1">
        <property type="nucleotide sequence ID" value="XM_017484052.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017339543.1">
        <property type="nucleotide sequence ID" value="XM_017484054.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SAQ9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021081"/>
    <dbReference type="GeneID" id="108274141"/>
    <dbReference type="KEGG" id="ipu:108274141"/>
    <dbReference type="CTD" id="558881"/>
    <dbReference type="GeneTree" id="ENSGT01010000222313"/>
    <dbReference type="OMA" id="IIASSCR"/>
    <dbReference type="OrthoDB" id="10266980at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050291">
        <property type="term" value="F:sphingosine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019216">
        <property type="term" value="P:regulation of lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050807">
        <property type="term" value="P:regulation of synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001964">
        <property type="term" value="P:startle response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050846">
        <property type="entry name" value="TLCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439:SF15">
        <property type="entry name" value="CERAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439">
        <property type="entry name" value="CT120 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013510292" evidence="7">
        <location>
            <begin position="18"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="8">
        <location>
            <begin position="34"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017339541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017339543.1"/>
        </source>
    </evidence>
    <sequence length="301" mass="33688" checksum="5BD0B5E028435949" modified="2017-12-20" version="1">MLSILAAGSVFFPGLFLLSKQCLKRLPGLGWHEGDAVIVSARLVSSVQAVMASSAGYIISTSCDDIIKDQHWLTTSYILFAVPYFVYDIYAMFLCYWHKLQVKGHEVDNGPKSKSVALSSYLRREFLMVLHHVVMVTVCFPVSVFWRQGKGDYFQGVMFLAELSTPSVCLGKILIQYKQQHTLLHKVNGALMLVTFFICRVLLFPYLYYVYGRYASIPFYQVPLAVPWQCNLGAALLMAPQLYWFCLICRGALRLFTGGSSRSRCPTSPSKLLLDGHTSFQTGNGYCGQAATLDPPTTTIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SBS9</accession>
    <name>A0A2D0SBS9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16 17 18 19 20" type="primary">mov10l1</name>
    </gene>
    <organism evidence="15 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16 17 18" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16 17 18">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA2/NAM7 helicase family. SDE3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017340187.1">
        <property type="nucleotide sequence ID" value="XM_017484698.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017340188.1">
        <property type="nucleotide sequence ID" value="XM_017484699.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017340189.1">
        <property type="nucleotide sequence ID" value="XM_017484700.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017340190.1">
        <property type="nucleotide sequence ID" value="XM_017484701.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017340191.1">
        <property type="nucleotide sequence ID" value="XM_017484702.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SBS9"/>
    <dbReference type="GeneID" id="108274500"/>
    <dbReference type="KEGG" id="ipu:108274500"/>
    <dbReference type="CTD" id="54456"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18078">
        <property type="entry name" value="DEXXQc_Mov10L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000864">
        <property type="entry name" value="Mov10-like RISC complex RNA helicase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049080">
        <property type="entry name" value="MOV-10-like_beta-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45418">
        <property type="entry name" value="CANCER/TESTIS ANTIGEN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45418:SF1">
        <property type="entry name" value="CANCER_TESTIS ANTIGEN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21634">
        <property type="entry name" value="MOV-10_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 16 17" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013510640" description="RNA helicase" evidence="11">
        <location>
            <begin position="23"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase MOV-10-like beta-barrel" evidence="14">
        <location>
            <begin position="574"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="12">
        <location>
            <begin position="760"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="12">
        <location>
            <begin position="873"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="13">
        <location>
            <begin position="951"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="701"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="701"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF21634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017340187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017340188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017340189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017340190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017340191.1"/>
        </source>
    </evidence>
    <sequence length="1203" mass="133055" checksum="E9359D59074D69C5" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SBV3</accession>
    <name>A0A2D0SBV3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">slc6a13</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017340214.1">
        <property type="nucleotide sequence ID" value="XM_017484725.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SBV3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000017596"/>
    <dbReference type="GeneID" id="108274519"/>
    <dbReference type="KEGG" id="ipu:108274519"/>
    <dbReference type="CTD" id="6540"/>
    <dbReference type="OMA" id="FTPAVCM"/>
    <dbReference type="OrthoDB" id="6581954at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005332">
        <property type="term" value="F:gamma-aminobutyric acid:sodium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11496">
        <property type="entry name" value="SLC6sbd-TauT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF111">
        <property type="entry name" value="SODIUM- AND CHLORIDE-DEPENDENT GABA TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00754">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="3 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="123"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="246"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="289"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="326"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="384"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="429"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="459"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="505"/>
            <end position="527"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="539"/>
            <end position="562"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
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    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="57"/>
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        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="64"/>
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        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="332"/>
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        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
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    <feature type="binding site" evidence="6">
        <location>
            <position position="397"/>
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        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
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            <name>Na(+)</name>
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        <location>
            <begin position="163"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017340214.1"/>
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    <sequence length="627" mass="71229" checksum="D2E924FD73B725FE" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SK09</accession>
    <name>A0A2D0SK09_ICTPU</name>
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        <recommendedName>
            <fullName evidence="9">Transketolase</fullName>
            <ecNumber evidence="8">2.2.1.1</ecNumber>
        </recommendedName>
    </protein>
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        <name evidence="17" type="primary">tktb</name>
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    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="16">SDA103</strain>
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    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
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    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the transketolase family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.1" evidence="8"/>
    <dbReference type="RefSeq" id="XP_017342826.1">
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    <dbReference type="OrthoDB" id="10267175at2759"/>
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        <property type="component" value="Chromosome 15"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:thiamine pyrophosphate binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="P:glyceraldehyde-3-phosphate metabolic process"/>
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        <property type="entry name" value="Transketolase isoform 1"/>
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        <property type="entry name" value="Transketolase isoform 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="transketolase isoform X2"/>
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    </dbReference>
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        <property type="entry name" value="THDP-binding"/>
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    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
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        <property type="entry name" value="Transketolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020826">
        <property type="entry name" value="Transketolase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049557">
        <property type="entry name" value="Transketolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004559">
        <property type="entry name" value="PRK05899.2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43195">
        <property type="entry name" value="TRANSKETOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43195:SF4">
        <property type="entry name" value="TRANSKETOLASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00456">
        <property type="entry name" value="Transketolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00801">
        <property type="entry name" value="TRANSKETOLASE_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00802">
        <property type="entry name" value="TRANSKETOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="15">
        <location>
            <begin position="319"/>
            <end position="483"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00007131"/>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017342826.1"/>
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    </evidence>
    <sequence length="626" mass="67911" checksum="081CFC490B4ED5CA" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SME2</accession>
    <name>A0A2D0SME2_ICTPU</name>
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        <recommendedName>
            <fullName evidence="7">Protein ILRUN</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC108276351</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017343450.1">
        <property type="nucleotide sequence ID" value="XM_017487961.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SME2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026082"/>
    <dbReference type="GeneID" id="108276351"/>
    <dbReference type="KEGG" id="ipu:108276351"/>
    <dbReference type="OrthoDB" id="661148at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14947">
        <property type="entry name" value="NBR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14349">
        <property type="entry name" value="UBA_CF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000015">
        <property type="entry name" value="Chromosome 6 C6orf106 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000289">
        <property type="entry name" value="Chromosome 6 open reading frame 106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039517">
        <property type="entry name" value="C6orf106_UBA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032350">
        <property type="entry name" value="Nbr1_FW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20930">
        <property type="entry name" value="OVARIAN CARCINOMA ANTIGEN CA125-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20930:SF0">
        <property type="entry name" value="PROTEIN ILRUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16158">
        <property type="entry name" value="N_BRCA1_IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nbr1 FW" evidence="9">
        <location>
            <begin position="112"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="235"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="247"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017343450.1"/>
        </source>
    </evidence>
    <sequence length="299" mass="32706" checksum="880DF7B8CD313498" modified="2017-12-20" version="1">MRTRFVVLFSSLGPKPRTKLETGAAVCCAAMEAMETDIDSELMQKFSCMGTTDKDILITEFQRLLGFQLNPAGCAFFLDMTNWNLQAAIGAYYDFESPSGSAPSMSFVKDVTIGEGESIPPDTPFIKTWRIQNTGSEAWPPGVCLRYTGGDQFGHVNFVMVRSLGPQEIHDVSVQMRSPCAPGMYQGQWRMCTAPGLFYGDVIWVILSVEVGGLLGVTQQLSSFQAEFNTQPHRSVHDDYNPFASPHNATQGDCSLTDSTPTQHTALSNSSVTTATNNLQNTIPATYDQPYGPHPFGQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SN92</accession>
    <name>A0A2D0SN92_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">C-type mannose receptor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108276672</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017343966.1">
        <property type="nucleotide sequence ID" value="XM_017488477.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SN92"/>
    <dbReference type="GeneID" id="108276672"/>
    <dbReference type="KEGG" id="ipu:108276672"/>
    <dbReference type="OrthoDB" id="6369810at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 20 MEMBER A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784:SF3">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 4 MEMBER K-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="1">
        <location>
            <begin position="93"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017343966.1"/>
        </source>
    </evidence>
    <sequence length="265" mass="30033" checksum="70CD72A6D4B78071" modified="2017-12-20" version="1">MAIIESNDNMIQLQNEAQRVQFSSSAWIGLYNDINSWRWSFGNEPLGSVKKWAGVEPNNSSGNQQCVALLTTGWTDRICTETLPFVCFDGNNTGNYSYTYFSNKKSWYDAQKYCRQYHTDLANTRDATEYSAVYNLVTPLTMFTWIGLFRDSWKWVDTTILSTISWMPGKSDNALGNENCGYLSNGQAVDTQCSDVMPFFCYSVITGMKQIISVKVRSSEDMNGAVIMAVTLEQLKQKLQDHGIAGDTTVKWREQPDGVVFHKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0STH6</accession>
    <name>A0A2D0STH6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein phosphatase 1 regulatory subunit 14A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">17 kDa PKC-potentiated inhibitory protein of PP1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Protein kinase C-potentiated inhibitor protein of 17 kDa</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ppp1r14ab</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PP1 inhibitor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017345811.1">
        <property type="nucleotide sequence ID" value="XM_017490322.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0STH6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000010496"/>
    <dbReference type="GeneID" id="108277502"/>
    <dbReference type="KEGG" id="ipu:108277502"/>
    <dbReference type="CTD" id="393783"/>
    <dbReference type="OMA" id="NKVHSPN"/>
    <dbReference type="OrthoDB" id="8193882at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004865">
        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.220:FF:000002">
        <property type="entry name" value="protein phosphatase 1 regulatory subunit 14A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.220">
        <property type="entry name" value="CPI-17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008025">
        <property type="entry name" value="CPI-17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036658">
        <property type="entry name" value="CPI-17_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16188">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 INHIBITOR POTENTIATED BY PROTEIN KINASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16188:SF4">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 14A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05361">
        <property type="entry name" value="PP1_inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81790">
        <property type="entry name" value="Myosin phosphatase inhibitor 17kDa protein, CPI-17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0650">Protein phosphatase inhibitor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00077289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017345811.1"/>
        </source>
    </evidence>
    <sequence length="148" mass="17251" checksum="44717B8C29D932FE" modified="2017-12-20" version="1">MAANRVGRRHNSKVHSPSRGSGRESGLSVQKRQARVTVKYNRKELQRRLDVEKWIDTGLDELYLGREEHMPEEVNIDELLDLQSDEERTKRLQDILHSCNTNTEVFIKELVLKLHGLQKQEDLHNDGIEHPQLHIFPDRQNSANSEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SWM4</accession>
    <name>A0A2D0SWM4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FAD-dependent oxidoreductase domain-containing protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">foxred2</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017347082.1">
        <property type="nucleotide sequence ID" value="XM_017491593.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SWM4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006602"/>
    <dbReference type="GeneID" id="108278313"/>
    <dbReference type="KEGG" id="ipu:108278313"/>
    <dbReference type="CTD" id="80020"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000521">
        <property type="entry name" value="FAD dependent oxidoreductase domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050982">
        <property type="entry name" value="Auxin_biosynth/cation_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539:SF23">
        <property type="entry name" value="FAD-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539">
        <property type="entry name" value="FLAVIN-BINDING MONOOXYGENASE-LIKE PROTEIN (AFU_ORTHOLOGUE AFUA_4G09220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13738">
        <property type="entry name" value="Pyr_redox_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017347082.1"/>
        </source>
    </evidence>
    <sequence length="715" mass="81152" checksum="B3A0868B0105A433" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SXY2</accession>
    <name>A0A2D0SXY2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">BarH-like homeobox 1b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">barhl1b</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the BAR homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347543.1">
        <property type="nucleotide sequence ID" value="XM_017492054.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SXY2"/>
    <dbReference type="GeneID" id="108278604"/>
    <dbReference type="KEGG" id="ipu:108278604"/>
    <dbReference type="CTD" id="553186"/>
    <dbReference type="OMA" id="QPTHEAS"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000097">
        <property type="entry name" value="barH-like 2 homeobox protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050848">
        <property type="entry name" value="Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24333">
        <property type="entry name" value="HOMEO BOX HB9 LIKE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24333:SF5">
        <property type="entry name" value="VENT HOMEOBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="186"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="188"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="153"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="319"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="162"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="328"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017347543.1"/>
        </source>
    </evidence>
    <sequence length="345" mass="37456" checksum="A8BF29FB36A636A5" modified="2017-12-20" version="1">MEASTHGSCSGSGFGIDSLLSHRPGSPVVVVAKGNGSLEGECRSPPEFSAASDADSVCSSPPSPRREEHEEDEEREAHEQRRGRALALPSHTHHGPVSAAPQPRTVTSSFLIRDILADCKPLAACAPYSSNGQPTHEASRLASKVADEFIEKIHSNSSSDGEYNKVKEEGDREISSSRDSPQVRLKKPRKARTAFTDHQLAQLERSFERQKYLSVQDRMELAASLNLTDTQVKTWYQNRRTKWKRQTAVGLELLAEAGNYSALQRMFPSPYFYPQGLVSGLDPGAAALSYLCRGAGAPPPSAPAALQRPPLVPRILLHGLQGGHEHPPPLPPLPGVVLPRPTPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T094</accession>
    <name>A0A2D0T094_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Uridylate-specific endoribonuclease</fullName>
            <ecNumber evidence="11">4.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">endou2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ribonucleotidyl-uridine-RNA = a 5'-end dephospho-uridine-RNA + a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA</text>
            <dbReference type="Rhea" id="RHEA:67792"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:17354"/>
            <dbReference type="Rhea" id="RHEA-COMP:17356"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:173117"/>
            <dbReference type="ChEBI" id="CHEBI:173224"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 11">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ENDOU family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_017348339.2">
        <property type="nucleotide sequence ID" value="XM_017492850.3"/>
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    <dbReference type="KEGG" id="ipu:108278996"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR018998">
        <property type="entry name" value="EndoU_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12439">
        <property type="entry name" value="PLACENTAL PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12439:SF32">
        <property type="entry name" value="URIDYLATE-SPECIFIC ENDORIBONUCLEASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09412">
        <property type="entry name" value="XendoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142877">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51959">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="EndoU" evidence="12">
        <location>
            <begin position="4"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU367085"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017348339.2"/>
        </source>
    </evidence>
    <sequence length="315" mass="36288" checksum="CEB28659EF18EA49" modified="2023-06-28" version="2">MTEHDPDLRAVVQEVWDNDINRLRPGTDYRISLQSKAANLSNLNDGQEAAGSPLFTYVDESIFKKETFLAFISLLDNYESDTGVPEVVTPEEEVENHRFLDSIMKTPVMKIVHKYLVEKNLSPLDTSAFKEQLHRIWFELYARRGSSKPDSSGFEHVFVGETRGGHTVIGFHNWIQLYLQEKLGHIDYKGYSVEANSPEPDENKHLLALQFSWKNGIKPKGSIFIGVSPEFEFALYTLCFITSPNERVKVSFSLYDVEIVCHHYNQKHIGTTYPVLIRYQDVQIFMISLRTSTSRYYESQITPVPRIDVRLLLCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T2G9</accession>
    <name>A0A2D0T2G9_ICTPU</name>
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        <recommendedName>
            <fullName evidence="2">BUD13 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">bud13</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CWC26 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017349006.2">
        <property type="nucleotide sequence ID" value="XM_017493517.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T2G9"/>
    <dbReference type="GeneID" id="108279337"/>
    <dbReference type="KEGG" id="ipu:108279337"/>
    <dbReference type="CTD" id="84811"/>
    <dbReference type="OrthoDB" id="6022at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070274">
        <property type="term" value="C:RES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018609">
        <property type="entry name" value="Bud13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051112">
        <property type="entry name" value="CWC26_splicing_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31809">
        <property type="entry name" value="BUD13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31809:SF0">
        <property type="entry name" value="BUD13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09736">
        <property type="entry name" value="Bud13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="126"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="536"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="33"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="159"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="279"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="303"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="324"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="433"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="548"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017349006.2"/>
        </source>
    </evidence>
    <sequence length="620" mass="71050" checksum="90B11931DDDBA468" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T2V6</accession>
    <name>A0A2D0T2V6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dual specificity protein phosphatase</fullName>
            <ecNumber evidence="6">3.1.3.16</ecNumber>
            <ecNumber evidence="6">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">dusp4</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="6"/>
    <dbReference type="EC" id="3.1.3.48" evidence="6"/>
    <dbReference type="RefSeq" id="XP_017348925.1">
        <property type="nucleotide sequence ID" value="XM_017493436.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T2V6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006484"/>
    <dbReference type="GeneID" id="108279297"/>
    <dbReference type="KEGG" id="ipu:108279297"/>
    <dbReference type="CTD" id="1846"/>
    <dbReference type="OMA" id="ELGEMDC"/>
    <dbReference type="OrthoDB" id="165342at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001706">
        <property type="term" value="P:endoderm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01446">
        <property type="entry name" value="DSP_MapKP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000015">
        <property type="entry name" value="Dual specificity phosphatase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008343">
        <property type="entry name" value="MKP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF111">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000939">
        <property type="entry name" value="MAPK_Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01764">
        <property type="entry name" value="MAPKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4 6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="10">
        <location>
            <begin position="20"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="8">
        <location>
            <begin position="168"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="9">
        <location>
            <begin position="230"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="7">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000939-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017348925.1"/>
        </source>
    </evidence>
    <sequence length="366" mass="39999" checksum="B94B280ABC3AEDCF" modified="2017-12-20" version="1">MEQLSEMECGVLKRLLKEDGTARCLVLDCRSFLAFSACHIRSAVNVRCNTIVRRRAKGSVSLDQILAGDEEVKSRLISGLYSAVILYDERTSDASALKQDSTIALVFNALGRDTFNTEVYLLKGGYDRFFSQYPDYCLKSKSLPMPAAQSSTETSCSSCATPQHDQGGPVEILPFLFLGSAFHASKKDMLDGMGISALLNVSSSCPNHFEGAYQYKCIPVEDNHKEDISSWFIEAIDFIDSVKDSNGRVLVHCQAGISRSATICLAYLMKKKRVCLDEAFEFVKQRRSIISPNFSFMGQLLQFESQVLATSCSVEAASPSATLGPKSSPSTSSPFIFSFPMGPVGPHGQPTNLSYLQSPITTSPSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T5A8</accession>
    <name>A0A2D0T5A8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor EC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">tfec</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MiT/TFE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017350092.1">
        <property type="nucleotide sequence ID" value="XM_017494603.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T5A8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021501"/>
    <dbReference type="GeneID" id="108279937"/>
    <dbReference type="KEGG" id="ipu:108279937"/>
    <dbReference type="CTD" id="22797"/>
    <dbReference type="OrthoDB" id="6242697at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18925">
        <property type="entry name" value="bHLHzip_TFEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000003">
        <property type="entry name" value="microphthalmia-associated transcription factor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021802">
        <property type="entry name" value="MiT/TFE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031867">
        <property type="entry name" value="MiT/TFE_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45776">
        <property type="entry name" value="MIP04163P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45776:SF1">
        <property type="entry name" value="TRANSCRIPTION FACTOR EC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11851">
        <property type="entry name" value="DUF3371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15951">
        <property type="entry name" value="MITF_TFEB_C_3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="13">
        <location>
            <begin position="232"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="337"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="387"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="441"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="285"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="341"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="389"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="451"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017350092.1"/>
        </source>
    </evidence>
    <sequence length="472" mass="52613" checksum="6F90082EC172C337" modified="2017-12-20" version="1">MLSQTENQTSRMFQGHSSVPVSLVRSGVNEQWNSGQWYSSYSYPSTSQNGTYHPTQPPSWSQPYSNWKVQNHLETSKYHLHQAQAQQVKQYLTLGTKLPGQAHPHPGQALGTMPVMRNGHMTPVSDNSTPGSPVTLFTLANSHDTEFPVDEVIDDLIGFESGFNDGSLECMEPSIIMQNNVALNSGMLEVYGGDQGMTASHGGLTPPSCPPKLSVKMEVTEHDTRVLAKERQKKDNHNLIERRRRYNINYRIKELGTLIPKSNDPDMRWNKGTILKASVEYIKWLQKEQQHARELESRQKKLEQANRRLLLRIQELEIQARAHGLPSNLGAAEISSHLLKQQQQQQQQQQQALPAPAVTPGAQTSLYPQDELNGPEYLQRNSLSVLHAGNEGNSSSSGEHADATSTTFSDPLSHFTDFFSATLKEEQRLDELLMEEALSPFGTDPLLSATSPNASKGSSRRSSFSTDDNDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T6X4</accession>
    <name>A0A2D0T6X4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">pyroxd1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Probable FAD-dependent oxidoreductase; involved in the cellular oxidative stress response. Required for normal sarcomere structure and muscle fiber integrity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. PYROXD1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017350385.1">
        <property type="nucleotide sequence ID" value="XM_017494896.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T6X4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013344"/>
    <dbReference type="GeneID" id="101154671"/>
    <dbReference type="KEGG" id="ipu:101154671"/>
    <dbReference type="CTD" id="79912"/>
    <dbReference type="OMA" id="MCENLIL"/>
    <dbReference type="OrthoDB" id="202203at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050260">
        <property type="entry name" value="FAD-bd_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041575">
        <property type="entry name" value="Rubredoxin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429:SF2">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18267">
        <property type="entry name" value="Rubredoxin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="8">
        <location>
            <begin position="12"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="8">
        <location>
            <begin position="251"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="NADH-rubredoxin oxidoreductase C-terminal" evidence="9">
        <location>
            <begin position="401"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017350385.1"/>
        </source>
    </evidence>
    <sequence length="487" mass="54230" checksum="E9F50A6B80BB9B9C" modified="2017-12-20" version="1">MAGSSLAEKHFRFVVVGGGISGVTCAEQLAFQFPKDEVALLTAAPLVKTVTNLKQVSKTLEEFDVEEQPSSVLEEKYPNLKVIHSAVRVLCAKQHVLQTEDGHNFCYEKLCVCTGARPKLLIENPHVLGIRDTDSAQDFQKKLCKAKRMIVIGNGGIALELVYEVEGCEVIWAIKDKAIGNTFFDAGAAQFLIPSLEVEKPEKTLPCKRARYTIDRAVNPKAGSLDHGSALGPDWHQGISLRGAEEVSHNVHVEYECEIEAVHSQEDFMQSPLYTQDSDLGTWPVYAQLTNGKVYGCDFIVSATGVVPNTEPFLQGNNFELAEDHGLRVDEHMRTSEPDVYAAGDVCSAGWEPSPLWQQMRLWTQARQMGFYAARCIAAHVLEEAIELDFCFELFSHITRFFNYKVVLLGKFNAQGLGLDHELLVRCTKGLEYVKVVLSRGRMVGAVLIGDTDLEETFENLILNQMDLSSYGEELLNPDIDIEDYFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0TBD2</accession>
    <name>A0A2D0TBD2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Histone-lysine N-methyltransferase SETMAR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">setmar</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017352020.1">
        <property type="nucleotide sequence ID" value="XM_017496531.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0TBD2"/>
    <dbReference type="SMR" id="A0A2D0TBD2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032517"/>
    <dbReference type="GeneID" id="108281008"/>
    <dbReference type="KEGG" id="ipu:108281008"/>
    <dbReference type="CTD" id="6419"/>
    <dbReference type="OMA" id="VDSMVPK"/>
    <dbReference type="OrthoDB" id="616263at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10544">
        <property type="entry name" value="SET_SETMAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050973">
        <property type="entry name" value="H3K9_Histone-Lys_N-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007728">
        <property type="entry name" value="Pre-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46223:SF3">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SET-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46223">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SUV39H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05033">
        <property type="entry name" value="Pre-SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00508">
        <property type="entry name" value="PostSET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00468">
        <property type="entry name" value="PreSET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50867">
        <property type="entry name" value="PRE_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Pre-SET" evidence="10">
        <location>
            <begin position="54"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="9">
        <location>
            <begin position="121"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="11">
        <location>
            <begin position="261"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017352020.1"/>
        </source>
    </evidence>
    <sequence length="284" mass="30739" checksum="243F2D1236FB6866" modified="2017-12-20" version="1">MTGLYGGDISNGLENVPVCIEKDVNEDSLPNFQYTPENVQGVGCDVDPSEVTLPGCSCRLASCVQPSCPCLRFGLVYTEGGLLEQREEPGASYSQPVFECNALCACGESCRSRVVQNGLQIRLCVFRTENRGWGVVTLEAIKRGSFVCEYAGEVIGFEEARRRQVSQTPEDKNYIIAVQEHGGSQRLGVTFVDPVAVGNVGRFLNHSCQPNLTMVPVRVHSVVPRLALFSSRDIEAGEELAFDYSGGHVQRTDASAAGALQRKVCLCGAQNCTGFLPLDLSVLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EL59</accession>
    <name>A0A979EL59_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15 16" type="primary">ptpn3</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15 16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="RefSeq" id="XP_047006092.1">
        <property type="nucleotide sequence ID" value="XM_047150136.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047006093.1">
        <property type="nucleotide sequence ID" value="XM_047150137.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EL59"/>
    <dbReference type="GeneID" id="108256378"/>
    <dbReference type="CTD" id="5774"/>
    <dbReference type="OrthoDB" id="5854685at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06706">
        <property type="entry name" value="PDZ_PTPN3-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000002">
        <property type="entry name" value="Band 4.1-like protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000045">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000003">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45706">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45706:SF5">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
        <property type="entry name" value="FERM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="12">
        <location>
            <begin position="31"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="13">
        <location>
            <begin position="473"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="10">
        <location>
            <begin position="610"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="11">
        <location>
            <begin position="782"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="379"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="398"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_047006092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_047006093.1"/>
        </source>
    </evidence>
    <sequence length="877" mass="99897" checksum="BA969D514A608056" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979ENE3</accession>
    <name>A0A979ENE3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dynactin subunit 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">dctn6</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the dynactin subunits 5/6 family. Dynactin subunit 6 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047008061.1">
        <property type="nucleotide sequence ID" value="XM_047152105.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979ENE3"/>
    <dbReference type="GeneID" id="100528597"/>
    <dbReference type="CTD" id="10671"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005869">
        <property type="term" value="C:dynactin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04646">
        <property type="entry name" value="LbH_Dynactin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027777">
        <property type="entry name" value="DCTN6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13072">
        <property type="entry name" value="DYNACTIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13072:SF0">
        <property type="entry name" value="DYNACTIN SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_047008061.1"/>
        </source>
    </evidence>
    <sequence length="187" mass="20747" checksum="D62C7AE4961E1F8C" modified="2023-02-22" version="1">MSRKPSCWVFIDCRLESCIQICRCVAGPRTVIHPKARIIADAGPIIIGEGNLIEEQALIINSYPENIMPDTEGYEPKTMTIGTNNVFEVGCVSQAMKIGDNNVIESKADVGRNVILTSGCIVGACCRLNTCEEIPENTVIYGSNCMRRVQTERPQPQTLQLDFLMKILPNYHHLKKTQKVNPTPAQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F1F8</accession>
    <name>A0A979F1F8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tetratricopeptide repeat protein 39C isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ttc39c</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TTC39 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047014028.1">
        <property type="nucleotide sequence ID" value="XM_047158072.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979F1F8"/>
    <dbReference type="GeneID" id="108270684"/>
    <dbReference type="CTD" id="125488"/>
    <dbReference type="OrthoDB" id="2154985at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032474">
        <property type="term" value="P:otolith morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019412">
        <property type="entry name" value="IML2/TPR_39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31859">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 39 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31859:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 39C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10300">
        <property type="entry name" value="Iml2-TPR_39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_047014028.1"/>
        </source>
    </evidence>
    <sequence length="577" mass="65175" checksum="D235B1763253A180" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F603</accession>
    <name>A0A979F603_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Rho GTPase-activating protein 23 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">arhgap23b</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047015407.1">
        <property type="nucleotide sequence ID" value="XM_047159451.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108273815"/>
    <dbReference type="CTD" id="562244"/>
    <dbReference type="OrthoDB" id="6281275at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06756">
        <property type="entry name" value="PDZ_ARHGAP21_23-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01253">
        <property type="entry name" value="PH_ARHGAP21-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04395">
        <property type="entry name" value="RhoGAP_ARHGAP21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000014">
        <property type="entry name" value="Rho GTPase activating protein 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000066">
        <property type="entry name" value="Rho GTPase activating protein 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23175">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23175:SF5">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="96"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="782"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="5">
        <location>
            <begin position="998"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="536"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="954"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1220"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1266"/>
            <end position="1442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1494"/>
            <end position="1528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="549"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="954"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="965"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1230"/>
            <end position="1245"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1334"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1376"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1396"/>
            <end position="1412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1430"/>
            <end position="1439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1500"/>
            <end position="1520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_047015407.1"/>
        </source>
    </evidence>
    <sequence length="1528" mass="169221" checksum="B0AE994824D1DE3D" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979FEF3</accession>
    <name>A0A979FEF3_ICTPU</name>
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        <recommendedName>
            <fullName evidence="3">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ptprz1b</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
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        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Receptor class 5 subfamily.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_047162163.2"/>
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    <dbReference type="GeneID" id="108280061"/>
    <dbReference type="KEGG" id="ipu:108280061"/>
    <dbReference type="CTD" id="553019"/>
    <dbReference type="OrthoDB" id="429145at2759"/>
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        <property type="component" value="Chromosome 19"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03122">
        <property type="entry name" value="alpha_CARP_receptor_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000016">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase gamma isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000313">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000013">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase zeta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041887">
        <property type="entry name" value="Alpha_CARP_receptor-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF461">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE ZETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="21" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5037640221" description="protein-tyrosine-phosphatase" evidence="15">
        <location>
            <begin position="25"/>
            <end position="1305"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="19">
        <location>
            <begin position="38"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="315"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="16">
        <location>
            <begin position="709"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="17">
        <location>
            <begin position="901"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="16">
        <location>
            <begin position="1015"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="17">
        <location>
            <begin position="1192"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="410"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="600"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1283"/>
            <end position="1305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="410"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="422"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="600"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_047018119.1"/>
        </source>
    </evidence>
    <sequence length="1305" mass="147166" checksum="C4A56C93F682FB31" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QY85</accession>
    <name>A0A9F7QY85_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytochrome c oxidase assembly protein COX16 homolog, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC108263232</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="11">Blood</tissue>
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        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
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        <text evidence="2">Belongs to the COX16 family.</text>
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        <property type="nucleotide sequence ID" value="XM_053679290.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
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        <property type="entry name" value="Cyt_c_Oxase_assmbl_COX16"/>
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    <dbReference type="PANTHER" id="PTHR17130:SF14">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX16 HOMOLOG, MITOCHONDRIAL"/>
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        <property type="entry name" value="COX16"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021814"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053535265.1"/>
        </source>
    </evidence>
    <sequence length="106" mass="12548" checksum="D23416DB04384BF4" modified="2023-06-28" version="1">MWKQMNKLQKNRTVRYGVPMLLLVVGGSFGLREFTQIRYDAQKIKKKMDPALEARVNSHTHTDILQDEYELKQADLDSWTNIRGPRPWENSRQSQEEQRTQLTKTT</sequence>
</entry>
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        <submittedName>
            <fullName evidence="5">Heparan-alpha-glucosaminide N-acetyltransferase</fullName>
        </submittedName>
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    <gene>
        <name evidence="5" type="primary">hgsnat</name>
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    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
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        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012429">
        <property type="entry name" value="HGSNAT_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31061:SF37">
        <property type="entry name" value="HEPARAN-ALPHA-GLUCOSAMINIDE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="LD22376P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07786">
        <property type="entry name" value="HGSNAT_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
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            <end position="51"/>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
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        <location>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="2">
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="581"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="618"/>
            <end position="636"/>
        </location>
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            <end position="370"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053533647.1"/>
        </source>
    </evidence>
    <sequence length="644" mass="72053" checksum="D2B038B942C16F63" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RDH7</accession>
    <name>A0A9F7RDH7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Interleukin enhancer-binding factor 3a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ilf3a</name>
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    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
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        <lineage>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
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            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Liu S."/>
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                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053540775.1">
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    <dbReference type="OrthoDB" id="8898434at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:precatalytic spliceosome"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.40:FF:000001">
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    <dbReference type="FunFam" id="3.30.460.10:FF:000003">
        <property type="entry name" value="interleukin enhancer-binding factor 3 isoform X2"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DZF_dom"/>
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        <property type="entry name" value="DZF_dom_N"/>
    </dbReference>
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        <property type="entry name" value="NT_sf"/>
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        <property type="entry name" value="INTERLEUKIN ENHANCER-BINDING FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45762">
        <property type="entry name" value="ZINC FINGER RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20965">
        <property type="entry name" value="DZF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07528">
        <property type="entry name" value="DZF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DSRM"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="DZF"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
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    </dbReference>
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        <property type="entry name" value="DS_RBD"/>
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    </dbReference>
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        <property type="entry name" value="DZF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DZF" evidence="8">
        <location>
            <begin position="79"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="7">
        <location>
            <begin position="428"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="7">
        <location>
            <begin position="544"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="522"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="713"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="716"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053540775.1"/>
        </source>
    </evidence>
    <sequence length="798" mass="87937" checksum="525FCDC23AF1C1E9" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RE62</accession>
    <name>A0A9F7RE62_ICTPU</name>
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        <submittedName>
            <fullName evidence="8">Uncharacterized protein si:dkey-30c15.2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">si:dkey-30c15.2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053533742.1">
        <property type="nucleotide sequence ID" value="XM_053677767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RE62"/>
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    <dbReference type="KEGG" id="ipu:108264169"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF36">
        <property type="entry name" value="SI:DKEY-30C15.2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="288"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="6">
        <location>
            <begin position="28"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053533742.1"/>
        </source>
    </evidence>
    <sequence length="353" mass="39862" checksum="11E0C72FB7BF0E6F" modified="2023-06-28" version="1">MNSSGLSDQEITALSSVYVSCLSLSLIGSCSVFITSIMKREKLKEQAKPLIQLALADLLASLCLGITAVMNLLWSGAVRNSEVVCSAGLALSLTFYSVSFLLVIVYACESAHSLQGWREEDEGDFTHQCPISQDSSSRRRRIDLLYVLVWLLPVIGFVVYIKTVSFSLTSLIPSQHASNYKQSAHNARFCNSCFLFLQLHIDDNEICSTVDLVHIKCIRIFTFISVLSVLISCTLMYWRLQVRLRRYEQDGVRIWSSARYMILVIIFSWTPALVLICLSFAFSETESLFPLFIIQAASVSLYGFLNSIVYAWRRRNFREAVLGERMPLLAYTHRAFFDQSLRNDAEETGSGQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RJ60</accession>
    <name>A0A9F7RJ60_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">guanylate cyclase</fullName>
            <ecNumber evidence="2">4.6.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">gucy1a2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.2" evidence="2"/>
    <dbReference type="RefSeq" id="XP_053543276.1">
        <property type="nucleotide sequence ID" value="XM_053687301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RJ60"/>
    <dbReference type="GeneID" id="108278235"/>
    <dbReference type="KEGG" id="ipu:108278235"/>
    <dbReference type="CTD" id="2977"/>
    <dbReference type="OrthoDB" id="6127067at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008074">
        <property type="term" value="C:guanylate cyclase complex, soluble"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004383">
        <property type="term" value="F:guanylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019934">
        <property type="term" value="P:cGMP-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070482">
        <property type="term" value="P:response to oxygen levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.260:FF:000002">
        <property type="entry name" value="guanylate cyclase soluble subunit alpha-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000007">
        <property type="entry name" value="Guanylate cyclase soluble subunit alpha-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1520.10">
        <property type="entry name" value="H-NOX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.260">
        <property type="entry name" value="Haem NO binding associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038158">
        <property type="entry name" value="H-NOX_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011644">
        <property type="entry name" value="Heme_NO-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011645">
        <property type="entry name" value="HNOB_dom_associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042463">
        <property type="entry name" value="HNOB_dom_associated_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45655:SF7">
        <property type="entry name" value="GUANYLATE CYCLASE SOLUBLE SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45655">
        <property type="entry name" value="GUANYLATE CYCLASE SOLUBLE SUBUNIT BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07700">
        <property type="entry name" value="HNOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07701">
        <property type="entry name" value="HNOBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0141">cGMP biosynthesis</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="11">
        <location>
            <begin position="529"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="258"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="470"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_053543276.1"/>
        </source>
    </evidence>
    <sequence length="743" mass="82458" checksum="54F93C18F9004794" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RQM3</accession>
    <name>A0A9F7RQM3_ICTPU</name>
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        <submittedName>
            <fullName evidence="7">Involucrin isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC128634214</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus lamina</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CRWN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053540477.1">
        <property type="nucleotide sequence ID" value="XM_053684502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RQM3"/>
    <dbReference type="GeneID" id="128634214"/>
    <dbReference type="KEGG" id="ipu:128634214"/>
    <dbReference type="OrthoDB" id="8963348at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005652">
        <property type="term" value="C:nuclear lamina"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006997">
        <property type="term" value="P:nucleus organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040418">
        <property type="entry name" value="CRWN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31908:SF11">
        <property type="entry name" value="PROTEIN CROWDED NUCLEI 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31908">
        <property type="entry name" value="PROTEIN CROWDED NUCLEI 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="82"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="305"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="305"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="345"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053540477.1"/>
        </source>
    </evidence>
    <sequence length="358" mass="42818" checksum="A3D86203F0DD103E" modified="2023-06-28" version="1">MADDSSDFCLSSPDTPETYDYCPVKEMGTATGNNPSQTIEDTTKQTCKIKKNKKEKKEKHLHMKVSYEKKEKNLEAREIAVEKRETHQNRREIHLEEREKHLNEKETNLAEKEKHLQQKEITLQEKGKHLDGKEVQFEEKEKHLDGKEMNLAEKEKHLQQRGIHLQEKEKHLDGKEVQFEEKEKQLFVREINLGKRSKYLEGRETRLQERTKHLEEKDISLEGSLKHLLGRENSLQERTKHLEEKEVSLDERLKHLLGRENSLQERGKCLDGREVQLEKREMRIDRRESRVDEREQRLEGKEIHLEEKEKNLAQHDLRHLNSADRENEKQERSSFMKSSKKQKREHPDEEEKEHSVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RSZ6</accession>
    <name>A0A9F7RSZ6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein CXorf65 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC128635274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053543268.1">
        <property type="nucleotide sequence ID" value="XM_053687293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RSZ6"/>
    <dbReference type="GeneID" id="128635274"/>
    <dbReference type="KEGG" id="ipu:128635274"/>
    <dbReference type="OrthoDB" id="8807723at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039471">
        <property type="entry name" value="CXorf65-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33887">
        <property type="entry name" value="PB1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33887:SF5">
        <property type="entry name" value="PB1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15874">
        <property type="entry name" value="Il2rg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053543268.1"/>
        </source>
    </evidence>
    <sequence length="254" mass="28898" checksum="90F2A1E249E670BA" modified="2023-06-28" version="1">MFITVLHGDDQKSLFNTQCKTVVLLDWIKVKCGCKNEAEIDLANLNGQLMNLPHHQFAVASDVLAQREEYILISISRTSDPSNPVHTSLLHNYDLHDPQCLAKLREEKTEKKPCASEEANGLSRRLPSSLSTESQPRPYKQIRFSSSPTFQTYSDKPVSTFPILHCGLQTWTTIPRALIHSYHAHLHPISHTITPQFKRIFEAFTLCKVLCVITIPHHKRLYPVPRFDSCSLILFLVSCSRFFPCLVYACLPIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RU18</accession>
    <name>A0A9F7RU18_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Scavenger receptor cysteine-rich type 1 protein M130-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC128635389</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053544121.1">
        <property type="nucleotide sequence ID" value="XM_053688146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RU18"/>
    <dbReference type="GeneID" id="128635389"/>
    <dbReference type="KEGG" id="ipu:128635389"/>
    <dbReference type="OrthoDB" id="536948at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000002">
        <property type="entry name" value="Scavenger receptor cysteine-rich type 1 protein M130"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000004">
        <property type="entry name" value="Scavenger receptor cysteine-rich type 1 protein M130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000009">
        <property type="entry name" value="WC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331:SF468">
        <property type="entry name" value="SCAVENGER RECEPTOR CYSTEINE-RICH TYPE 1 PROTEIN M160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331">
        <property type="entry name" value="SCAVENGER RECEPTOR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="624"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="12">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="12">
        <location>
            <begin position="41"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="12">
        <location>
            <begin position="147"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="12">
        <location>
            <begin position="258"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="12">
        <location>
            <begin position="359"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="12">
        <location>
            <begin position="487"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="747"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="755"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="6"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="172"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="185"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="216"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="325"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="384"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="397"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="428"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="512"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="556"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_053544121.1"/>
        </source>
    </evidence>
    <sequence length="795" mass="85692" checksum="F6FA6380286815DC" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TDP4</accession>
    <name>A0A9F7TDP4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Phosphatidylserine synthase</fullName>
            <ecNumber evidence="15">2.7.8.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Serine-exchange enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ptdss1b</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes a base-exchange reaction in which the polar head group of phosphatidylethanolamine (PE) is replaced by L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + L-serine = a 1,2-diacyl-sn-glycero-3-phospho-L-serine + ethanolamine</text>
            <dbReference type="Rhea" id="RHEA:27606"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="EC" id="2.7.8.29"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:27607"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Phospholipid metabolism; phosphatidylserine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Endoplasmic reticulum membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the phosphatidyl serine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.29" evidence="15"/>
    <dbReference type="RefSeq" id="XP_053531363.1">
        <property type="nucleotide sequence ID" value="XM_053675388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TDP4"/>
    <dbReference type="GeneID" id="108257397"/>
    <dbReference type="CTD" id="559442"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106245">
        <property type="term" value="F:L-serine-phosphatidylethanolamine phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006659">
        <property type="term" value="P:phosphatidylserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004277">
        <property type="entry name" value="PSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362:SF33">
        <property type="entry name" value="PHOSPHATIDYLSERINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03034">
        <property type="entry name" value="PSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 15" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10 15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 15" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="13 15" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="222"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="329"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="358"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_053531363.1"/>
        </source>
    </evidence>
    <sequence length="367" mass="42808" checksum="0554665E39649111" modified="2023-06-28" version="1">MVFGLSVLYFLFLVFLIFLNWDQVKLLMYWLDPNLRYATREADVMEYAVNCHMITWERILSHFDIFAFGHFWGWAMKALLIRSYGLCWTISITWELTELFFMHLLPNFAECWWDQVILDILLCNGGGIWLGMTVCHFLEMRTYRWASIKDIHSATGKLKRAVLQFTPASWTYVRWFDPKSSFQRVAGIYLFMIIWQLTELNTFFLKHIFVFQASHPLSWCRILFVGIITAPTVRQYYAYLTDTQCKRVGTQCWVFGAIAFLEALACVKFGQDLFSKTQVMYVILWLVCVAFITSLCLYGMVWHEQKYGGRLKLQSTSDCDDSSFIDSSGYTPDTIKGERDSSNSQLLHQRKGGPVKNKATNGTNNKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TER1</accession>
    <name>A0A9F7TER1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein LOC108255570 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC108255570</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053530477.1">
        <property type="nucleotide sequence ID" value="XM_053674502.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="108255570"/>
    <dbReference type="OrthoDB" id="8900349at2759"/>
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        <property type="component" value="Chromosome 22"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039918928" evidence="2">
        <location>
            <begin position="35"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="3">
        <location>
            <begin position="36"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="3">
        <location>
            <begin position="155"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053530477.1"/>
        </source>
    </evidence>
    <sequence length="456" mass="51381" checksum="B9B6074F44A049CB" modified="2023-06-28" version="1">MKPTTPSRAMQSCSVHHCVLLLLTLTFTTAPVLSAVCSKTVKLHQPVTLSCEYKCSGSVKWSLFNNRDVVFAGCDETSCSSLVKGYNMSYDQYQDGDLSLTITAADYRMRNTYSCECGVSEISTVRLSIETVFSAVQVKHDGDLILDLTVPEPVEVIYTSSDSADGEWICNVTQHSLQCKAEYTHRTSLRYPELTLRDVTHSDSGRYTIRDTENKEDIRVYSVSVTDEKFPRWIIGLIVPVVVILLVTLLFLCIKYQKNAVHQEFIKVSKQLEHVNKLIQQARPGKEQGNKRHVGATEAVQKAEEAMKELERRYRESPKYSEQIKLFCKAKRAELMECSGEEGEGLLMTERGGIMGKMEEVMKSLQQLSGDGAGHLQGTSSDIISETEINLTDIEQWCSQKRAELNNFRQQHTAGAEWLFYGDTVINQHGADESSTWRQSGSIVFTLIIVNTYIKY</sequence>
</entry>
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    <accession>A0A9F7TI30</accession>
    <name>A0A9F7TI30_ICTPU</name>
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            <fullName evidence="3">DNA-directed RNA polymerase II subunit RPB1-like</fullName>
        </submittedName>
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        <name evidence="3" type="primary">LOC128629544</name>
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        <name type="scientific">Ictalurus punctatus</name>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053534002.1">
        <property type="nucleotide sequence ID" value="XM_053678027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TI30"/>
    <dbReference type="GeneID" id="128629544"/>
    <dbReference type="KEGG" id="ipu:128629544"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053534002.1"/>
        </source>
    </evidence>
    <sequence length="144" mass="15543" checksum="3A253A7F9C824F00" modified="2023-06-28" version="1">MGVRHFSLSPLLEGLDLDLNTRYQLAHLTADITMLLNVLRTSSGHAGFPPPLRYRIRPANVCIDTSTQTDPLPDPSSSYQTDDDVAFSDLGSDHSPPFSPASPEYSPPFSPASPEYSPPYTSAGYPTSPGCTPFYHPFSPTALG</sequence>
</entry>
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    <accession>A0A9F7TJI4</accession>
    <name>A0A9F7TJI4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">LOW QUALITY PROTEIN: uncharacterized protein LOC108271638</fullName>
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    </protein>
    <gene>
        <name evidence="8" type="primary">LOC108271638</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053539519.1">
        <property type="nucleotide sequence ID" value="XM_053683544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TJI4"/>
    <dbReference type="GeneID" id="108271638"/>
    <dbReference type="KEGG" id="ipu:108271638"/>
    <dbReference type="OrthoDB" id="8959642at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF118">
        <property type="entry name" value="CMRF35-LIKE MOLECULE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="6">
        <location>
            <begin position="41"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053539519.1"/>
        </source>
    </evidence>
    <sequence length="325" mass="36240" checksum="8C15D9766188AEBC" modified="2023-06-28" version="1">MIRELTVEDTGTYQCGVDKLGIDIYTPVELKVKEDLSYEKSISKTVHVGGDLNISCKYPESLRSDPKILCKTRLQRAACSYKESVKESRKYLNKGKFSLYDDRETQMFTVSIRNVTEQDSGEYWCGAEAAWTSDHGYQVYFTQIDLTVTGEFVETWRHVVSVSISEVPHRENVHCIVLHLCCYQSFTRDSLNVFHYLTKLFTALEPRVPVSASRPTQPSSLSSSSESTPASPPAGFPTSIVITVSVILLLLLIGIIFLFVILHKKCRIQAGTASIGECSVQGSGNDQEEVKDTRRLSASDAGKIYCNVQLPTIPSDPPQNVYANM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TK76</accession>
    <name>A0A9F7TK76_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Probable aminopeptidase NPEPL1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">npepl1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M17 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053540209.1">
        <property type="nucleotide sequence ID" value="XM_053684234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TK76"/>
    <dbReference type="GeneID" id="108273206"/>
    <dbReference type="CTD" id="79716"/>
    <dbReference type="OrthoDB" id="412814at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00433">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10590">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011356">
        <property type="entry name" value="Leucine_aapep/pepB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041417">
        <property type="entry name" value="NPEPL1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000819">
        <property type="entry name" value="Peptidase_M17_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963:SF4">
        <property type="entry name" value="AMINOPEPTIDASE NPEPL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963">
        <property type="entry name" value="LEUCINE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18295">
        <property type="entry name" value="Pdase_M17_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00883">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00481">
        <property type="entry name" value="LAMNOPPTDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00631">
        <property type="entry name" value="CYTOSOL_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytosol aminopeptidase" evidence="5">
        <location>
            <begin position="339"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053540209.1"/>
        </source>
    </evidence>
    <sequence length="525" mass="56617" checksum="1E4D89E0986670BA" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TQA3</accession>
    <name>A0A9F7TQA3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein LOC124627623 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC124627623</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053541847.1">
        <property type="nucleotide sequence ID" value="XM_053685872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TQA3"/>
    <dbReference type="GeneID" id="124627623"/>
    <dbReference type="KEGG" id="ipu:124627623"/>
    <dbReference type="OrthoDB" id="8871851at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF111">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039927766" evidence="9">
        <location>
            <begin position="28"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="19"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053541847.1"/>
        </source>
    </evidence>
    <sequence length="230" mass="26018" checksum="53C03DF1161CA9C8" modified="2023-06-28" version="1">MAKLLSTFLLQMLFGFFTPCFLEIASTDHLSVRPGENVTLLCNITNYSEISWYRLRSEEVKLLISAEKSKLKKTFSLSYNVNKSHFDITESSSSVTLVIIGVRETDLGFYFCGGRNRTTHIQFGKFIRLDFTADQHHETDQYDEIDYSYTFPNPQHSGLTHCGIIIIILSCVCAVFVSISIICSSLYCSTLQGENLQPEDQHLHRSPCHTHFPLFTTLPVVPGRAAALIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TQV0</accession>
    <name>A0A9F7TQV0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="29">Acyltransferase</fullName>
            <ecNumber evidence="29">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="31" type="primary">dgat2</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30">SDA103</strain>
        </source>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="31">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycerol + (9Z)-octadecenoyl-CoA = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:38219"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:38220"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycerol + hexadecanoyl-CoA = 1,2-di-(9Z)-octadecenoyl-3-hexadecanoyl-sn-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:38163"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:75583"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:38164"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>1,3-di-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoyl-CoA = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:38435"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:75735"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:38436"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>1-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoyl-CoA = 1,2-di-(9Z-octadecenoyl)-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:37915"/>
            <dbReference type="ChEBI" id="CHEBI:52323"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:75342"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:37916"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>1-O-(9Z-octadecenyl)-glycerol + (9Z)-octadecenoyl-CoA = 1-O-(9Z-octadecyl)-3-(9Z-octadecenoyl)-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:55340"/>
            <dbReference type="ChEBI" id="CHEBI:34116"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:197429"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:55341"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>2,3-di-(9Z)-octadecenoyl-sn-glycerol + (9Z)-octadecenoyl-CoA = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:38439"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:75824"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:38440"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>2-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoyl-CoA = 1,2-di-(9Z-octadecenoyl)-sn-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:37911"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:73990"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:37912"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2-(9Z-octadecenoyl)-glycerol + hexadecanoyl-CoA = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:38071"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:73990"/>
            <dbReference type="ChEBI" id="CHEBI:75466"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:38072"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>all-trans-retinol + an acyl-CoA = an all-trans-retinyl ester + CoA</text>
            <dbReference type="Rhea" id="RHEA:11488"/>
            <dbReference type="ChEBI" id="CHEBI:17336"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:63410"/>
            <dbReference type="EC" id="2.3.1.76"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:11489"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>all-trans-retinol + hexadecanoyl-CoA = all-trans-retinyl hexadecanoate + CoA</text>
            <dbReference type="Rhea" id="RHEA:38175"/>
            <dbReference type="ChEBI" id="CHEBI:17336"/>
            <dbReference type="ChEBI" id="CHEBI:17616"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:38176"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>an acyl-CoA + a 1,2-diacyl-sn-glycerol = a triacyl-sn-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:10868"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:64615"/>
            <dbReference type="EC" id="2.3.1.20"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:10869"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Glycerolipid metabolism; triacylglycerol biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
            <location evidence="7">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5 29">Endoplasmic reticulum membrane</location>
            <topology evidence="5 29">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 29">Belongs to the diacylglycerol acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="29">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="29"/>
    <dbReference type="RefSeq" id="XP_053542537.1">
        <property type="nucleotide sequence ID" value="XM_053686562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TQV0"/>
    <dbReference type="GeneID" id="100528806"/>
    <dbReference type="CTD" id="84649"/>
    <dbReference type="OMA" id="IMGVACT"/>
    <dbReference type="OrthoDB" id="264532at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004144">
        <property type="term" value="F:diacylglycerol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050252">
        <property type="term" value="F:retinol O-fatty-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07987">
        <property type="entry name" value="LPLAT_MGAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007130">
        <property type="entry name" value="DAGAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317:SF14">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03982">
        <property type="entry name" value="DAGAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="21" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17 29" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="16" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="19" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="20 29" id="KW-0472">Membrane</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14 29" id="KW-0808">Transferase</keyword>
    <keyword evidence="15 29" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18 29" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00004771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00005420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00048634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00049168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00049549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="RuleBase" id="RU367023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="RefSeq" id="XP_053542537.1"/>
        </source>
    </evidence>
    <sequence length="361" mass="40490" checksum="C3DC386E3A75791C" modified="2023-06-28" version="1">MKTLLAAYSGVKKGSGSWFLSALHDLPSAPWLSRSKLVKQLQAISVLQFVLSFLALGIAATVLLLYLLCTDCWVISAVYIAWLIFDWNTPKQGGRRSAWVRNWTVWTYFRDYFPVTLIKTHNLPPNRNYIFGYHPHGIFCFGAFCNFSTEATGFSKKFPGIKPSLATLAGNFRLPLLRDYLMSGGICPVNRNSIDYLLSCNGTGNAVVIVVGGAAESLNCTPGINSVTLRNRKGFIKLALQKGADLVPVYSFGENESYKQVIFDEGSRWRWVQRKLQKLLGFAPCIFHGCGFFFTESWGIVPYGKPINTVVGEPITVPKLEEPTPDVVEMYHTMYIKSLTTLFDKYKTRFGLKESDVLLID</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TRC7</accession>
    <name>A0A9F7TRC7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Heat shock factor protein 5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC128635157</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the HSF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053542479.1">
        <property type="nucleotide sequence ID" value="XM_053686504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TRC7"/>
    <dbReference type="GeneID" id="128635157"/>
    <dbReference type="KEGG" id="ipu:128635157"/>
    <dbReference type="OrthoDB" id="6418155at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000349">
        <property type="entry name" value="Heat shock transcription factor, Y-linked"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF278">
        <property type="entry name" value="HEAT SHOCK FACTOR PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="8">
        <location>
            <begin position="13"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053542479.1"/>
        </source>
    </evidence>
    <sequence length="294" mass="33642" checksum="7513A2428D07B59B" modified="2023-06-28" version="1">MENTDATFGTFINPNYFPGKLWRLVNDPQICSVWWDDSGEGILIHQETFEAEVLLSQPTHLSEYFRTADFISFIRQLNLYGFKKQRTNISDKQSYNPSIKAQLHHFHNPYFKRDKPELLLDIKRRTPLNKAKLAGLKVTGRTPRRFHHVMQNSAQETSGLMRTGSVLLEHQGTPYHHPCNVPQQEQRSNTPHHSQYGFYTPVYQRCSPDVLDSTVPCSQQPAASCSPSGYHPDYSVGHADPIDQDPYWRAGDVPESRTSDLGIEKEELDAILELALDMQDEVDVRTLLQLSLTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3TDY3</accession>
    <name>E3TDY3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP synthase subunit C lysine N-methyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Protein N-lysine methyltransferase FAM173B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">F173B</name>
        <name evidence="15" type="synonym">atpsckmt</name>
        <name evidence="15" type="synonym">f173b</name>
        <name evidence="15" type="synonym">fam173b</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ANT/ATPSC lysine N-methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="GU588563">
        <property type="protein sequence ID" value="ADO28519.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187342.1">
        <property type="nucleotide sequence ID" value="NM_001200413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TDY3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006664"/>
    <dbReference type="GeneID" id="100528143"/>
    <dbReference type="KEGG" id="ipu:100528143"/>
    <dbReference type="CTD" id="134145"/>
    <dbReference type="OrthoDB" id="66144at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904058">
        <property type="term" value="P:positive regulation of sensory perception of pain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905706">
        <property type="term" value="P:regulation of mitochondrial ATP synthesis coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000141">
        <property type="entry name" value="ATP synthase c subunit lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026170">
        <property type="entry name" value="FAM173A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13610:SF8">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT C LYSINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13610">
        <property type="entry name" value="METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00078098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADO28519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="NP_001187342.1"/>
        </source>
    </evidence>
    <sequence length="250" mass="27803" checksum="29875046E6A11803" modified="2011-01-11" version="1">MRQYSTVRTAECTLIKEAVWKLAADSSKQRSSAMSDLRVEIEKEPVQGDTNRSRVKNRVGLVATCALGGSLVALYAVAGPFIAPALRKTCLPFVPATNTRVKNVLRALQSRSGTLVDIGSGDGRIVIAAAREGFRSVGFELNPWLVWYSRYRAWKQGVHRHTSFYISDLWKVSFAQYSNVVIFGVPQMMEQLEAKLQTELQSSARVVACRFPFPTWTPDHVTGEGIDTVWVYDAETFKSHTTTAKAAEPQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2015-03-04" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>Q6QMG1</accession>
    <name>TSHR_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thyrotropin receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Thyroid-stimulating hormone receptor</fullName>
            <shortName>TSH-R</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tshr</name>
    </gene>
    <organism>
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2009" name="Gen. Comp. Endocrinol." volume="161" first="313" last="319">
            <title>Molecular cloning, characterization and expression of thyroid-stimulating hormone receptor in channel catfish.</title>
            <authorList>
                <person name="Goto-Kazeto R."/>
                <person name="Kazeto Y."/>
                <person name="Trant J.M."/>
            </authorList>
            <dbReference type="PubMed" id="19523396"/>
            <dbReference type="DOI" id="10.1016/j.ygcen.2009.01.009"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [MRNA]</scope>
        <scope>FUNCTION</scope>
        <scope>TISSUE SPECIFICITY</scope>
        <scope>INDUCTION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2 6">Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin (PubMed:19523396). Also acts as a receptor for the heterodimeric glycoprotein hormone thyrostimulin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Plays a central role in controlling thyroid cell metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="tissue specificity">
        <text evidence="6">Expressed in ventral aorta, testis and post-vitellogenic ovary (at protein level). Also expressed in kidney. Detected in unfertilized, but not fertilized eggs.</text>
    </comment>
    <comment type="induction">
        <text evidence="6">Ovarian expression levels tend to be low throughout vitellogenesis. Expression levels increase at the anticipated spawning period, and gradually decrease afterwards until the onset of ovarian recrudescence.</text>
    </comment>
    <comment type="similarity">
        <text>Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AY533543">
        <property type="protein sequence ID" value="AAS45557.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187121.1">
        <property type="nucleotide sequence ID" value="NM_001200192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q6QMG1"/>
    <dbReference type="SMR" id="Q6QMG1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007395"/>
    <dbReference type="GlyCosmos" id="Q6QMG1">
        <property type="glycosylation" value="3 sites, No reported glycans"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSIPUT00015027906">
        <property type="protein sequence ID" value="ENSIPUP00015022408"/>
        <property type="gene ID" value="ENSIPUG00015012635"/>
    </dbReference>
    <dbReference type="GeneID" id="100304626"/>
    <dbReference type="KEGG" id="ipu:100304626"/>
    <dbReference type="CTD" id="7253"/>
    <dbReference type="GeneTree" id="ENSGT00940000156510"/>
    <dbReference type="OMA" id="TRDMRQS"/>
    <dbReference type="OrthoDB" id="5981530at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004996">
        <property type="term" value="F:thyroid-stimulating hormone receptor activity"/>
        <property type="evidence" value="ECO:0000315"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904588">
        <property type="term" value="P:cellular response to glycoprotein"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905229">
        <property type="term" value="P:cellular response to thyrotropin-releasing hormone"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007276">
        <property type="term" value="P:gamete generation"/>
        <property type="evidence" value="ECO:0000270"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044752">
        <property type="term" value="P:response to human chorionic gonadotropin"/>
        <property type="evidence" value="ECO:0000315"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097066">
        <property type="term" value="P:response to thyroid hormone"/>
        <property type="evidence" value="ECO:0000315"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038194">
        <property type="term" value="P:thyroid-stimulating hormone signaling pathway"/>
        <property type="evidence" value="ECO:0000315"/>
        <property type="project" value="AgBase"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000019">
        <property type="entry name" value="Lutropin-choriogonadotropic hormone receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002131">
        <property type="entry name" value="Gphrmn_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026906">
        <property type="entry name" value="LRR_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002274">
        <property type="entry name" value="TSH_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372">
        <property type="entry name" value="GLYCOPROTEIN HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24372:SF0">
        <property type="entry name" value="THYROTROPIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13306">
        <property type="entry name" value="LRR_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00373">
        <property type="entry name" value="GLYCHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01145">
        <property type="entry name" value="TSHRECEPTOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0297">G-protein coupled receptor</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-0433">Leucine-rich repeat</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0675">Receptor</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0807">Transducer</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000431959" description="Thyrotropin receptor" evidence="3">
        <location>
            <begin position="19"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="8">
        <location>
            <begin position="19"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=1" evidence="3">
        <location>
            <begin position="421"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="442"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=2" evidence="3">
        <location>
            <begin position="451"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="8">
        <location>
            <begin position="472"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=3" evidence="3">
        <location>
            <begin position="495"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="516"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=4" evidence="3">
        <location>
            <begin position="540"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="8">
        <location>
            <begin position="561"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=5" evidence="3">
        <location>
            <begin position="582"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="603"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=6" evidence="3">
        <location>
            <begin position="626"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="8">
        <location>
            <begin position="647"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=7" evidence="3">
        <location>
            <begin position="661"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="682"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="repeat" description="LRR 1" evidence="3">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="repeat" description="LRR 2" evidence="3">
        <location>
            <begin position="99"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="repeat" description="LRR 3" evidence="3">
        <location>
            <begin position="124"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="repeat" description="LRR 4" evidence="3">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="repeat" description="LRR 5" evidence="3">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="repeat" description="LRR 6" evidence="3">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="repeat" description="LRR 7" evidence="3">
        <location>
            <begin position="224"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="744"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="PDZ-binding" evidence="1">
        <location>
            <begin position="775"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="744"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="1">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="1">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="1">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="494"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P16473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P21463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3"/>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="19523396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="7">
        <source>
            <dbReference type="PubMed" id="19523396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="8"/>
    <sequence length="778" mass="87360" checksum="553E040DCE540955" modified="2004-07-05" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5U6N5</accession>
    <name>W5U6N5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4 6">Myotubularin-related protein 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">mtmr10</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="JT406087">
        <property type="protein sequence ID" value="AHH37445.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017314190.1">
        <property type="nucleotide sequence ID" value="XM_017458701.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U6N5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034656"/>
    <dbReference type="GeneID" id="108259321"/>
    <dbReference type="KEGG" id="ipu:108259321"/>
    <dbReference type="CTD" id="54893"/>
    <dbReference type="OMA" id="SVPQDRF"/>
    <dbReference type="OrthoDB" id="271628at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022587">
        <property type="entry name" value="MTMR12-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF39">
        <property type="entry name" value="MYOTUBULARIN-RELATED PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12578">
        <property type="entry name" value="3-PAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="3">
        <location>
            <begin position="206"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="688"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AHH37445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017314190.1"/>
        </source>
    </evidence>
    <sequence length="765" mass="87107" checksum="EA524D9E74F65E2B" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5UAT9</accession>
    <name>W5UAT9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Chromobox homolog 7a</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="8">Chromobox protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CBX7</name>
        <name evidence="10 11" type="synonym">cbx7a</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT410484">
        <property type="protein sequence ID" value="AHH39078.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017337421.1">
        <property type="nucleotide sequence ID" value="XM_017481932.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017337422.1">
        <property type="nucleotide sequence ID" value="XM_017481933.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UAT9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033754"/>
    <dbReference type="GeneID" id="108273000"/>
    <dbReference type="KEGG" id="ipu:108273000"/>
    <dbReference type="CTD" id="550551"/>
    <dbReference type="OMA" id="TTEERCE"/>
    <dbReference type="OrthoDB" id="1918685at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035102">
        <property type="term" value="C:PRC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18646">
        <property type="entry name" value="CD_Cbx7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000006">
        <property type="entry name" value="Chromobox protein homolog 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043000">
        <property type="entry name" value="CBX7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033773">
        <property type="entry name" value="CBX7_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017984">
        <property type="entry name" value="Chromo_dom_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47277">
        <property type="entry name" value="CHROMOBOX PROTEIN HOMOLOG 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47277:SF1">
        <property type="entry name" value="CHROMOBOX PROTEIN HOMOLOG 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17218">
        <property type="entry name" value="CBX7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00504">
        <property type="entry name" value="CHROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Chromo" evidence="7">
        <location>
            <begin position="11"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="141"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="218"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="311"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="165"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="188"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="240"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="324"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AHH39078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017337421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017337422.1"/>
        </source>
    </evidence>
    <sequence length="397" mass="44710" checksum="F8F029F9EB7AD320" modified="2014-04-16" version="1">MELSSIGEQVFAVESITKKRVRKGNVEYLLKWQGWPPKYSTWEPEDNILDPRLVLAFEEKEERDRAQAHRRKGLRPRRLVLRNIYAMELRSAQKTSEKPAPRFRLSLARSMGAGLQHNARRCRNGEGGVYHRLLKRRSRQFVPKSLESAESPRQRTLTLEKNAAEEDEQEKEWEKEEEQQEKKKRRKTENEEHHDIPHGPEMTEDHVLSTQETVITDGKPEHCASSPDCEVKPPSTEIGSATTAADQSASTTITNTYEHKPITDTSGVGVTQDQPTTDKSLNSSSGVGVITDRLQNRASVITVRFGSIDRSAQDEEKETGETDSDAKRETDKDEVTAECTTTLQLSAEVRTDESQASECTRHPGKVIVTNVTINSLTVTFKEATAAEGFFSSYGLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5UBJ4</accession>
    <name>W5UBJ4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Voltage-dependent L-type calcium channel subunit beta-3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Calcium channel voltage-dependent subunit beta 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Calcium channel voltage-dependent subunit beta 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Voltage-dependent L-type calcium channel subunit beta-4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">CACNB4</name>
        <name evidence="19" type="synonym">cacnb4b</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Regulatory subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents. Increases CACNA1B peak calcium current and shifts the voltage dependencies of channel activation and inactivation. Increases CACNA1C peak calcium current and shifts the voltage dependencies of channel activation and inactivation.</text>
    </comment>
    <comment type="function">
        <text evidence="1">The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of a calcium channel complex consisting of a pore-forming alpha subunit (CACNA1C) and the ancillary subunits CACNB3 and CACNA2D1. The channel complex contains alpha, beta, gamma and delta subunits in a 1:1:1:1 ratio. Interacts with CACNA2D4. Interacts with FASLG. Interacts with CBARP; prevents the interaction of CACNB3 with the alpha subunit CACNA1C thereby negatively regulating the activity of the corresponding calcium channel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">The L-type calcium channel is composed of four subunits: alpha-1, alpha-2, beta and gamma. Interacts with FASLG. Interacts with CBARP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calcium channel beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="JT410183">
        <property type="protein sequence ID" value="AHH38959.1"/>
        <property type="molecule type" value="mRNA"/>
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        <property type="nucleotide sequence ID" value="XM_017480777.3"/>
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    <dbReference type="AlphaFoldDB" id="W5UBJ4"/>
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    <dbReference type="CTD" id="678560"/>
    <dbReference type="GeneTree" id="ENSGT00950000182837"/>
    <dbReference type="OrthoDB" id="5962384at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit beta-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit beta-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046937">
        <property type="entry name" value="CAB1-4_N_A-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008079">
        <property type="entry name" value="VDCC_L_b3su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000584">
        <property type="entry name" value="VDCC_L_bsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11824">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12052">
        <property type="entry name" value="VGCC_beta4Aa_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01626">
        <property type="entry name" value="LCACHANNELB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01696">
        <property type="entry name" value="LCACHANNELB3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="62"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="14"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="136"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="385"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="33"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="388"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="406"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00030522"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00045432"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00046590"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00064941"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00073550"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00082362"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AHH38959.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017336266.1"/>
        </source>
    </evidence>
    <sequence length="484" mass="54134" checksum="D56D19719682E2A7" modified="2014-04-16" version="1">MYDNLYLHGFEDSEAGSADSYTSRPSDSDVSLEEERQDVGSQGRQEREQQAAVQLERAKAKAVAFAVKTNVSYCGALDEDVPVPGTAISFDAKDFLHIKEKFNNDWWIGRLVKEGCEIGFIPSPLKLENIRAQLEQKKSRVQGKSSGSSSSSLGDVMSSGKQKQKVTEHVPPYDVVPSMRPVVLVGPSLKGYEVTDMMQKALFDFLKHRFDGRISITRVTADISLAKRSVLNNPSKRAIIERSNTRSSLAEVQSEIERIFELARSLQLVVLDADTINHPAQLLKTSLAPIIVHVKVSSPKVLQRLIKSRGKSQSKHLNVQLVAADKLAQCPPEMFDIILDENQLEDACEHLAEYLEAYWKATHTSSSTPLNPLLGRNLGPSALAPYPSTISGLQNQQGQRTRHANHTGDHSTANERRNLMTPDENYHNERPHKGRGNRSSSGSQQGREHHHYMDEQQDPYQDSYKPHRNRSSPGTYSQDSHHRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5UDG0</accession>
    <name>W5UDG0_ICTPU</name>
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        <submittedName>
            <fullName evidence="3 5">Biogenesis of lysosome-related organelles complex 1 subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">bloc1s2</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BLOC1S2 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT412955">
        <property type="protein sequence ID" value="AHH40061.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017311376.1">
        <property type="nucleotide sequence ID" value="XM_017455887.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UDG0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019886"/>
    <dbReference type="GeneID" id="108257817"/>
    <dbReference type="KEGG" id="ipu:108257817"/>
    <dbReference type="CTD" id="282991"/>
    <dbReference type="GeneTree" id="ENSGT00390000005889"/>
    <dbReference type="OMA" id="CSDMFEK"/>
    <dbReference type="OrthoDB" id="244061at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031083">
        <property type="term" value="C:BLOC-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099078">
        <property type="term" value="C:BORC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032418">
        <property type="term" value="P:lysosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019269">
        <property type="entry name" value="BLOC1_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46479">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46479:SF1">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10046">
        <property type="entry name" value="BLOC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AHH40061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017311376.1"/>
        </source>
    </evidence>
    <sequence length="177" mass="19794" checksum="7F2A8510BA212C4E" modified="2014-04-16" version="1">MAAMGQEAAAMDSISRAPPSVLQLRSDTDSGAEPGHEEASENVAPAPRRPTNNNDGGVETAEEATEPAEPDINELCKDMFEKMAVFLQGELTATCEDYKLLENMNKLTSLKYMEMKDISINISRNLQDLNQKYASLQPYLDQINQIEEQVTALEQAAYKLDTYSKKLEAKFKKLEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5UE13</accession>
    <name>W5UE13_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">3-oxoacyl-[acyl-carrier-protein] synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">OXSM</name>
        <name evidence="23" type="synonym">oxsm</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">May play a role in the biosynthesis of lipoic acid as well as longer chain fatty acids required for optimal mitochondrial function.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a fatty acyl-[ACP] + malonyl-[ACP] + H(+) = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.41"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:22837"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>butanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxohexanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41820"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9628"/>
            <dbReference type="Rhea" id="RHEA-COMP:9629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78454"/>
            <dbReference type="ChEBI" id="CHEBI:78456"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:41821"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>decanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxododecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41868"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9640"/>
            <dbReference type="Rhea" id="RHEA-COMP:9641"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78468"/>
            <dbReference type="ChEBI" id="CHEBI:78469"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:41869"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>dodecanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxotetradecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41884"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9644"/>
            <dbReference type="Rhea" id="RHEA-COMP:9645"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:65264"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78473"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:41885"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>hexanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxooctanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9632"/>
            <dbReference type="Rhea" id="RHEA-COMP:9633"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78459"/>
            <dbReference type="ChEBI" id="CHEBI:78460"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:41837"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>octanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxodecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41852"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78463"/>
            <dbReference type="ChEBI" id="CHEBI:78464"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:41853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tetradecanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxohexadecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41900"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9648"/>
            <dbReference type="Rhea" id="RHEA-COMP:9649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78477"/>
            <dbReference type="ChEBI" id="CHEBI:78478"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:41901"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 17 19">Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.</text>
    </comment>
    <dbReference type="EMBL" id="JT412297">
        <property type="protein sequence ID" value="AHH39794.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017309963.1">
        <property type="nucleotide sequence ID" value="XM_017454474.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UE13"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006866"/>
    <dbReference type="GeneID" id="108257067"/>
    <dbReference type="KEGG" id="ipu:108257067"/>
    <dbReference type="CTD" id="54995"/>
    <dbReference type="GeneTree" id="ENSGT00940000157768"/>
    <dbReference type="OMA" id="QIGHCLG"/>
    <dbReference type="OrthoDB" id="5334845at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000015">
        <property type="entry name" value="3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000024">
        <property type="entry name" value="3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017568">
        <property type="entry name" value="3-oxoacyl-ACP_synth-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03150">
        <property type="entry name" value="fabF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005589">
        <property type="entry name" value="PRK07314.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712:SF336">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="POLYKETIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000447">
        <property type="entry name" value="KAS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7 17" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3 17" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17 19" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="20">
        <location>
            <begin position="45"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="active site" description="For beta-ketoacyl synthase activity" evidence="18">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00054575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000447-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="AHH39794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017309963.1"/>
        </source>
    </evidence>
    <sequence length="465" mass="48866" checksum="6C4CCEBDEA560CD2" modified="2014-04-16" version="1">MSVACWQKLPVRQFTRVLSNTRVLTCIGVQTFERLNSEHGPTRCLRRVVITGIGLVCPLGTGTGLPWERLIAGESGIVSLDPDEYKAVPCKVAARVPRGQGDGEFKEEMFASRGEINSMSQATVMALGAAQLALQDCGWYPGSPEEQLSAGVAVGMGMVPLEEIASTAAAFQTRGYSKVSPFFVPRILVNMAAGHISIKHKLKGPNHAVSTACTTGAHAIGDATRFIIHGDATVMLAGGTEACVSPLAIAGFARARALSTSWNEQPKLASRPFHPQREGFVMGEGAAMLVLEEYKHAVERGARVYAEVLGYGLSGDASHITAPSADGDGAFRCMSAALRDAGVSPSDVTYVNAHATSTPLGDAAENAAVKRLFQQHSQLLAVSSTKGATGHLLGAAGALEAAFTALACYHAILPPTLNLDRTEPEFDLNYVPCAAQPWRTGGRRVALTNSFGFGGTNASLCLASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5UFG0</accession>
    <name>W5UFG0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="19 21">Nucleotide-binding oligomerization domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">NOD2</name>
        <name evidence="21" type="synonym">nod2</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the NOD1-NOD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT409680">
        <property type="protein sequence ID" value="AHH38778.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JT413507">
        <property type="protein sequence ID" value="AHH40274.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017314821.1">
        <property type="nucleotide sequence ID" value="XM_017459332.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UFG0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000023997"/>
    <dbReference type="GeneID" id="100379166"/>
    <dbReference type="KEGG" id="ipu:100379166"/>
    <dbReference type="CTD" id="64127"/>
    <dbReference type="GeneTree" id="ENSGT00940000160934"/>
    <dbReference type="OMA" id="HCCWPDA"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043122">
        <property type="term" value="P:regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060333">
        <property type="term" value="P:type II interferon-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000940">
        <property type="entry name" value="Nucleotide-binding oligomerization domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
        <property type="entry name" value="CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00619">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00114">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0391">Immunity</keyword>
    <keyword evidence="6" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0564">Palmitate</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="CARD" evidence="17">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="CARD" evidence="17">
        <location>
            <begin position="108"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="18">
        <location>
            <begin position="272"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00038296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AHH40274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017314821.1"/>
        </source>
    </evidence>
    <sequence length="981" mass="109952" checksum="BCE46510557BEBC5" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5UHH0</accession>
    <name>W5UHH0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Battenin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CLN3</name>
        <name evidence="11" type="synonym">cln3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Lysosome membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the battenin family.</text>
    </comment>
    <dbReference type="EMBL" id="JT410575">
        <property type="protein sequence ID" value="AHH39117.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017344106.1">
        <property type="nucleotide sequence ID" value="XM_017488617.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UHH0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000024412"/>
    <dbReference type="GeneID" id="108276732"/>
    <dbReference type="CTD" id="1201"/>
    <dbReference type="OrthoDB" id="5965864at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007040">
        <property type="term" value="P:lysosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003492">
        <property type="entry name" value="Battenin_disease_Cln3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018460">
        <property type="entry name" value="Battenin_disease_Cln3_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10981">
        <property type="entry name" value="BATTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10981:SF0">
        <property type="entry name" value="BATTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02487">
        <property type="entry name" value="CLN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015974">
        <property type="entry name" value="CLN3_BTN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01315">
        <property type="entry name" value="BATTENIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-0458">Lysosome</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="371"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AHH39117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017344106.1"/>
        </source>
    </evidence>
    <sequence length="437" mass="48256" checksum="7CBF3C7E56DA6CC5" modified="2014-04-16" version="1">MDHRDSVNADPDMLTGSEGRCRRCRNQSGFWLLGLCNNFAYVVMLSAAHDILKQQESQNATMPTPGPVPSDGGNSSNSSRYDCNPVSTAAVLLADILPTLLIKLTAPFFIHKVPYGFRILGCVATATTSFLVVSFSSTVWMSILGVIFASVSSGLGELSFLSLTVYFSSDVLSGWGSGTGGAGVAGAVLYSAFTQAGLSPRGTLLIMLLIPAVLMISYFRLLDFPPSFPQWRSGSGEDVQERRPLIVDDNDEDSVRDVNGKEDQSTEPLTFLDKLHILKRLLKFTVPLCVVYFAEYFINQGLLELLYFPASSLSHAEQYRWYQTLYQIGVFISRTSLYCVKTRRIWVLSMLQCVNAVVLLISVYYRFWPNVWVVFVIVLYEGLLGGAVYVNTFYLIRVETVEREREFAMAAASVGDSIGIALSAAPAIPIHDYFCSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5UJD7</accession>
    <name>W5UJD7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5 7 8">TRAF family member-associated NF-kappa-B activator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TANK</name>
        <name evidence="7 8" type="synonym">tank</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7 8">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT415735">
        <property type="protein sequence ID" value="AHH41805.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017325568.1">
        <property type="nucleotide sequence ID" value="XM_017470079.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047011898.1">
        <property type="nucleotide sequence ID" value="XM_047155942.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UJD7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021601"/>
    <dbReference type="GeneID" id="108266579"/>
    <dbReference type="CTD" id="10010"/>
    <dbReference type="GeneTree" id="ENSGT00390000008712"/>
    <dbReference type="OMA" id="KMKEHYE"/>
    <dbReference type="OrthoDB" id="9937252at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043124">
        <property type="term" value="P:negative regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="TANK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024581">
        <property type="entry name" value="TBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15249">
        <property type="entry name" value="TRAF FAMILY MEMBER-ASSOCIATED NF-KAPPA-B ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRAF FAMILY MEMBER-ASSOCIATED NF-KAPPA-B ACTIVATOR"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF12845">
        <property type="entry name" value="TBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
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    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tbk1/Ikki binding" evidence="4">
        <location>
            <begin position="130"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="9"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AHH41805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017325568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_047011898.1"/>
        </source>
    </evidence>
    <sequence length="344" mass="38404" checksum="7A179A0BFD6B3F5F" modified="2014-04-16" version="1">MDRNIGDPLNKAYEAYRNISIENENAKRQLQEKTEQFHQYTQQLEKKIEDQEQEISALKAQLNSIKCASGEVKCCETAHRKQETERLSPSIQWPSNSSTSHRTNYILNESLEFTSMRAHPVPGISGMKIEDVLDTLQDIQGTFQRIQTLARRQKDHLKRIHKGNDTASVSAEKQFSMPIQCTDDTVEQVEEPFSPPLRPEVDEGLISGALASRGASPEDGDFMDVVKFPPPTDSEYEFLHSAPDRGLPIPEPRKDLSEPISTVLEESYSSFPVSTPASHLLPASASHEGVRGPQQPLWSPELIPAIASETSSMGSAHCTFCKATVPSDLMYSHLNSHFQDKEGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5UK29</accession>
    <name>W5UK29_ICTPU</name>
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    <gene>
        <name evidence="9" type="primary">Zbtb7c</name>
        <name evidence="11 12" type="synonym">LOC108279258</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
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    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11 12">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT417516">
        <property type="protein sequence ID" value="AHH42407.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017348834.1">
        <property type="nucleotide sequence ID" value="XM_017493345.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017348835.1">
        <property type="nucleotide sequence ID" value="XM_017493346.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UK29"/>
    <dbReference type="GeneID" id="108279258"/>
    <dbReference type="GeneTree" id="ENSGT00940000159814"/>
    <dbReference type="OMA" id="HLDMERN"/>
    <dbReference type="OrthoDB" id="8922241at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
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    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger and BTB domain containing 7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000259">
        <property type="entry name" value="Zinc finger and BTB domain containing 7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000572">
        <property type="entry name" value="Zinc finger and BTB domain containing 7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF7">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING PROTEIN 7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="7">
        <location>
            <begin position="34"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="403"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="431"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="459"/>
            <end position="486"/>
        </location>
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            <begin position="487"/>
            <end position="522"/>
        </location>
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        <location>
            <begin position="133"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="507"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="550"/>
            <end position="580"/>
        </location>
    </feature>
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        <location>
            <begin position="143"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="182"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="235"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="507"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AHH42407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017348834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017348835.1"/>
        </source>
    </evidence>
    <sequence length="631" mass="71189" checksum="A75A5B85D21A4127" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5UK40</accession>
    <name>W5UK40_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">mRNA decay activator protein ZFP36</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Zinc finger protein 36</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ZFP36L1</name>
        <name evidence="16" type="synonym">zfp36l1a</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Zinc-finger RNA-binding protein that destabilizes several cytoplasmic AU-rich element (ARE)-containing mRNA transcripts by promoting their poly(A) tail removal or deadenylation, and hence provide a mechanism for attenuating protein synthesis. Acts as a 3'-untranslated region (UTR) ARE mRNA-binding adapter protein to communicate signaling events to the mRNA decay machinery. Functions by recruiting the CCR4-NOT deadenylase complex and probably other components of the cytoplasmic RNA decay machinery to the bound ARE-containing mRNAs, and hence promotes ARE-mediated mRNA deadenylation and decay processes. Binds to 3'-UTR ARE of numerous mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Associates with the cytoplasmic CCR4-NOT deadenylase complex to trigger ARE-containing mRNA deadenylation and decay processes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT412909">
        <property type="protein sequence ID" value="AHH40042.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017332026.1">
        <property type="nucleotide sequence ID" value="XM_017476537.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UK40"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035317"/>
    <dbReference type="GeneID" id="108270152"/>
    <dbReference type="KEGG" id="ipu:108270152"/>
    <dbReference type="CTD" id="561280"/>
    <dbReference type="GeneTree" id="ENSGT00940000155076"/>
    <dbReference type="OMA" id="YYFRPMS"/>
    <dbReference type="OrthoDB" id="410307at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061158">
        <property type="term" value="P:3'-UTR-mediated mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900153">
        <property type="term" value="P:positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000002">
        <property type="entry name" value="Zinc finger protein 36, C3H1 type-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007635">
        <property type="entry name" value="Tis11B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF177">
        <property type="entry name" value="MRNA DECAY ACTIVATOR PROTEIN ZFP36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04553">
        <property type="entry name" value="Tis11B_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="144"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="182"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="144"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="182"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="102"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="239"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="288"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="324"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AHH40042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017332026.1"/>
        </source>
    </evidence>
    <sequence length="372" mass="40379" checksum="E1B36EEE5D0B6AD0" modified="2014-04-16" version="1">MTTAVVPPLFDFSEVINNKNNKMLNYNNNILGAPHTMPVPCTGVNLTISNPTGSLLDRKAVGTPSIGGVYQRRHSVTLPSSKLNQNQFLSNGKADLALVGPGMGNSSNKENRLRDRSFSETGDRLMQKCTGSGGTGSSQVNSSRYKTELCRPFEENGACKYGDKCQFAHGIHELRSLSRHPKYKTELCRTFHTIGFCPYGPRCHFIHNAEERRGPPAAPSPLSTSNKLERPRLQHSYSFAGFPSSGGLRDSPTSVTPPPMFSPDELSEWPSSNPFTYSSQELVNLFGPSLSSGSGADHTVHAPSSPSNTPYSFRPMSESPHLFESPSSQPDSLSDQEGYQSSSGGSLSGSESPILDTTRRLPIFSRLSISDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5UKN4</accession>
    <name>W5UKN4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Unconventional myosin-Id</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">MYO1D</name>
        <name evidence="24" type="synonym">myo1d</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Perikaryon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 19">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="EMBL" id="JT407126">
        <property type="protein sequence ID" value="AHH37867.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JT417096">
        <property type="protein sequence ID" value="AHH42255.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017339355.1">
        <property type="nucleotide sequence ID" value="XM_017483866.3"/>
    </dbReference>
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        <property type="component" value="Chromosome 13"/>
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        <property type="term" value="C:myosin complex"/>
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        <property type="term" value="F:microfilament motor activity"/>
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        <property type="term" value="P:epithelial cilium movement involved in determination of left/right asymmetry"/>
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        <property type="term" value="P:heart jogging"/>
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        <property type="term" value="P:protein transport"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="MYSc_Myo1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Unconventional myosin ID"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Unconventional myosin ID"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Unconventional myosin-Id"/>
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        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
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        <property type="entry name" value="Kinesin_motor_dom_sf"/>
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        <property type="entry name" value="Myosin_head_motor_dom-like"/>
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        <property type="entry name" value="Myosin_TH1"/>
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        <property type="entry name" value="MYSc_Myo1"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
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    </dbReference>
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        <property type="entry name" value="UNCONVENTIONAL MYOSIN-ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
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        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="16 19" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="11 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="17" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="15 19" id="KW-0505">Motor protein</keyword>
    <keyword evidence="14 19" id="KW-0518">Myosin</keyword>
    <keyword evidence="9 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Myosin motor" evidence="20">
        <location>
            <begin position="9"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="domain" description="TH1" evidence="21">
        <location>
            <begin position="812"/>
            <end position="1003"/>
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        <location>
            <begin position="572"/>
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        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="102"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00039641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="AHH42255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_017339355.1"/>
        </source>
    </evidence>
    <sequence length="1004" mass="116102" checksum="1361A368797B5538" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5UL01</accession>
    <name>W5UL01_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Nuclear factor of activated T-cells 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">T-cell transcription factor NFAT5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">NFAT5</name>
        <name evidence="26" type="synonym">nfat5a</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="24">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">SDA103</strain>
        </source>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3'. Mediates the transcriptional response to hypertonicity. Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17. Also involved in the DNA damage response by preventing formation of R-loops; R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Homodimer when bound to DNA, completely encircles its DNA target. Interacts with CIDEC; this interaction is direct and retains NFAT5 in the cytoplasm. Does not bind with Fos and Jun transcription factors. Interacts with DDX5 and DDX17; this interaction leads to DDX5/DDX17 recruitment to LNC2 and S100A4 promoters and NFAT5-mediated DDX5/DDX17-enhanced transactivation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT413661">
        <property type="protein sequence ID" value="AHH40342.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017340570.1">
        <property type="nucleotide sequence ID" value="XM_017485081.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000024766"/>
    <dbReference type="GeneID" id="108274760"/>
    <dbReference type="KEGG" id="ipu:108274760"/>
    <dbReference type="CTD" id="334390"/>
    <dbReference type="GeneTree" id="ENSGT00940000155213"/>
    <dbReference type="OMA" id="LPMGQQS"/>
    <dbReference type="OrthoDB" id="5346094at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="P:anatomical structure morphogenesis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:calcineurin-NFAT signaling cascade"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:DNA damage response"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0060429">
        <property type="term" value="P:epithelium development"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:gene expression"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902531">
        <property type="term" value="P:regulation of intracellular signal transduction"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="CDD" id="cd07882">
        <property type="entry name" value="RHD-n_TonEBP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nuclear factor of activated T-cells 5, tonicity-responsive"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Nuclear factor of activated T-cells 5, tonicity-responsive"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_E-set"/>
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        <property type="entry name" value="IPT_dom"/>
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        <property type="entry name" value="NFAT"/>
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        <property type="entry name" value="NFAT5_RHD_DNA-bd"/>
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        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="RHD_dimer"/>
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        <property type="entry name" value="RHD_DNA_bind_dom"/>
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        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
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        <property type="entry name" value="NFAT"/>
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    </dbReference>
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        <property type="entry name" value="NUCLEAR FACTOR OF ACTIVATED T-CELLS 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16179">
        <property type="entry name" value="RHD_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="NUCFACTORATC"/>
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        <property type="entry name" value="IPT"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="14" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="21" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RHD" evidence="23">
        <location>
            <begin position="275"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="115"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="192"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="927"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="988"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1103"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1231"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="21">
        <location>
            <begin position="883"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="938"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="988"/>
            <end position="1005"/>
        </location>
    </feature>
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            <begin position="1006"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="1111"/>
            <end position="1122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00055141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00065799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00072227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00080722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="AHH40342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_017340570.1"/>
        </source>
    </evidence>
    <sequence length="1446" mass="156596" checksum="0E47A71833F61CB0" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5UL06</accession>
    <name>W5UL06_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">KH domain-containing, RNA-binding, signal transduction-associated protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Src-associated in mitosis 68 kDa protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">Khdrbs1</name>
        <name evidence="24" type="synonym">khdrbs1b</name>
    </gene>
    <organism evidence="22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="22">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the KHDRBS family.</text>
    </comment>
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        <property type="protein sequence ID" value="AHH42360.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_017482049.3"/>
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    <dbReference type="GeneID" id="108273069"/>
    <dbReference type="KEGG" id="ipu:108273069"/>
    <dbReference type="CTD" id="406516"/>
    <dbReference type="OMA" id="GYDENYT"/>
    <dbReference type="OrthoDB" id="6777263at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000036">
        <property type="entry name" value="KH RNA binding domain containing, signal transduction associated 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032571">
        <property type="entry name" value="Qua1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032335">
        <property type="entry name" value="Sam68-YY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF30">
        <property type="entry name" value="KH DOMAIN-CONTAINING, RNA-BINDING, SIGNAL TRANSDUCTION-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16274">
        <property type="entry name" value="Qua1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16568">
        <property type="entry name" value="Sam68-YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 19" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="12" id="KW-0729">SH3-binding</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="K Homology" evidence="21">
        <location>
            <begin position="57"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="182"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="322"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="20">
        <location>
            <begin position="187"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="20">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="341"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00070445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00078343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="AHH42360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_017337538.1"/>
        </source>
    </evidence>
    <sequence length="350" mass="39035" checksum="B6605BBDBB5FBBA4" modified="2014-04-16" version="1">MENENKYLPQLLAERDSLDSSFMHAMKLLSAEIERVQKDEPEKDNETYLDLFTAKNIKLKERVLIPVKQYPKFNFVGKILGPQGNTIKRLQEETGAKISVLGKGSMRDKAKEESLRKSGEPKYAHLSMELHVFIEVFAPAPEAYMRMAHAMEEVKKFLFPDMMDEICQEQFMEMGYLNGSHEHSVRGRGGPMARGRGGPPPPRGRGMAPMRGGVPRGGPARGGAVRGAPAGRGGPPAQPGRGAVANRARAPAPGSQRMPPPPRPPAQESYEDYSYDESYAESGYESYDNYYSQPQAEPEYYDYGHGETQEGYENYAQDDWNGTQRASASGKAAAQRQTKGAYREHPYGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5UL15</accession>
    <name>W5UL15_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ADP-ribosylation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">arf4</name>
        <name evidence="17" type="synonym">arf4a</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">GTP-binding protein involved in protein trafficking; modulates vesicle budding and uncoating within the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="EMBL" id="JT417412">
        <property type="protein sequence ID" value="AHH42370.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017306002.1">
        <property type="nucleotide sequence ID" value="XM_017450513.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UL15"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000010385"/>
    <dbReference type="GeneID" id="108254969"/>
    <dbReference type="KEGG" id="ipu:108254969"/>
    <dbReference type="CTD" id="445196"/>
    <dbReference type="GeneTree" id="ENSGT00940000156878"/>
    <dbReference type="OMA" id="SASEXTE"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04150">
        <property type="entry name" value="Arf1_5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000024">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045872">
        <property type="entry name" value="Arf1-5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 14" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 14" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10 14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 14" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU369003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AHH42370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017306002.1"/>
        </source>
    </evidence>
    <sequence length="180" mass="20455" checksum="E0B1F73097336897" modified="2014-04-16" version="1">MGLTISSVFSRLFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERAQEAAEELQKMLQEDELRDAVLLVFANKQDLPNAMAISELTDKLGLQTLRSRTWYVQATCATQGTGLYEGLDWLSEQLSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>W5ULH9</accession>
    <accession>A0A2D0RVC1</accession>
    <name>W5ULH9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Aquaporin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Aqp12</name>
        <name evidence="11" type="synonym">aqp12</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2023-07" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MIP/aquaporin (TC 1.A.8) family. AQP11/AQP12 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="JT418862">
        <property type="protein sequence ID" value="AHH42877.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017334448.2">
        <property type="nucleotide sequence ID" value="XM_017478959.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ULH9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000037219"/>
    <dbReference type="GeneID" id="108271384"/>
    <dbReference type="KEGG" id="ipu:108271384"/>
    <dbReference type="CTD" id="208760"/>
    <dbReference type="OMA" id="MIKNLMA"/>
    <dbReference type="OrthoDB" id="1580043at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000018">
        <property type="entry name" value="Aquaporin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051883">
        <property type="entry name" value="AQP11/12_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016697">
        <property type="entry name" value="Aquaporin_11/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023265">
        <property type="entry name" value="Aquaporin_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21191">
        <property type="entry name" value="AQUAPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21191:SF8">
        <property type="entry name" value="AQUAPORIN-12A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017529">
        <property type="entry name" value="Aquaporin_11/12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02025">
        <property type="entry name" value="AQUAPORIN12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="183"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="231"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR017529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AHH42877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017334448.2"/>
        </source>
    </evidence>
    <sequence length="280" mass="30934" checksum="4C7C3E91A969D1AE" modified="2014-04-16" version="1">MSGLNITLGYFISVVVVSGFAKALLTQWRPRWNFLSEFIAAFALAACRLEVQTISEIGQWAGGLGQDVTLTMLFLALTVHGVIMQGVTGNPSVTVMGYLQKETGAVSSSLSIAGQFGGAQLALLFAGWYWTMELTDMHMIKVMMMTQCSSSLNVSLTQGTITEVVSALLFHLVDLSLRRRSKLLRIPILALLLTFLYYTGNTYTSAYVNPSLAYALTFNCPGHTFWEYAVVYWLGPLIGMTLALLLYMGNIPLLFTKNLLFSKKAHLRLPKRKTSEEKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PL85</accession>
    <name>A0A2D0PL85_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Arrestin domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">zgc:110626</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arrestin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017306754.1">
        <property type="nucleotide sequence ID" value="XM_017451265.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PL85"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019731"/>
    <dbReference type="GeneID" id="108255345"/>
    <dbReference type="KEGG" id="ipu:108255345"/>
    <dbReference type="OrthoDB" id="2333384at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF135">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING 3-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="2">
        <location>
            <begin position="200"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017306754.1"/>
        </source>
    </evidence>
    <sequence length="345" mass="39291" checksum="FC51C253726A91A9" modified="2017-12-20" version="1">MNPTTFFSGPKTMACKVKKISVAFDPLNESSTFTNGDYISGRVTLEVVKQTHIESLFVKAKGKASVLWSENHGKYSTSVYYDKETCFKSIQYFIEENKRNGEDGYSLLTNESGQCYSRTVEPGIHVYPFTFQIPRHDLPASFKGCHGKVVYFLEAKLSRSMSMSKKAKVHFNYVPRGDMSIPYLMMPQHGYKEKKFKFLTSASVAMDVYTEKMGFHLGEEMKVKIDIVNNSSRTVKPKFSLYQKQSFFASKKRKVHTKVVLKEKGDRIDPSTKQSVTKVLKIPPDTCVSILNCRVLKVEYKLKVYLDIKFARDPQIKLPLVIFPAYVTQEDKDVANTEMGIGSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PMT9</accession>
    <name>A0A2D0PMT9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Kinetochore protein Nuf2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">nuf2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NUF2 family.</text>
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    <dbReference type="RefSeq" id="XP_017306385.1">
        <property type="nucleotide sequence ID" value="XM_017450896.2"/>
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    <dbReference type="GeneID" id="108255163"/>
    <dbReference type="KEGG" id="ipu:108255163"/>
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    <dbReference type="OrthoDB" id="8194677at2759"/>
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        <property type="component" value="Chromosome 21"/>
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    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:kinetochore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:meiotic chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.60">
        <property type="entry name" value="Ncd80 complex, Nuf2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005549">
        <property type="entry name" value="Kinetochore_Nuf2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038275">
        <property type="entry name" value="Nuf2_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21650:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN NUF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21650">
        <property type="entry name" value="MEMBRALIN/KINETOCHORE PROTEIN NUF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03800">
        <property type="entry name" value="Nuf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="11" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinetochore protein Nuf2 N-terminal" evidence="13">
        <location>
            <begin position="15"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="163"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="320"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="387"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005498"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF03800"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017306385.1"/>
        </source>
    </evidence>
    <sequence length="465" mass="54114" checksum="4B80C408E2898CC2" modified="2017-12-20" version="1">MAKKNLETDGAMMENTFPIYKVDAIVQFFRTEVLTGQEAKHFAKGDITPNPKMDVLQRLYMRILELVYRFRPDCHYLVPLSENLQHPMLHEWSTPILNLYACMCQFLPVCHVYDFSLNDLLNPKPKRTITILSGIQNFLHFRKHRLEIMAGHQQAFRADMDKLQSFTKGIKEAEKKIEKLMTIPPEQQAEAKELAAALTELKNATVQETQAVNALNEQVTECKREIAEQSQKLKQKKLEVATHKDEIAKLKSQIVESPEELKNEMEKMKESIKNIKISKELADERLVGLQIEVQCTSQGEAEIQVILKQLHDLQASMCKTNQQKEEVQNLVALYESLQKELKGLSNEEGQLKRALAMKYDKQSKQQIRHQKKKELKEHRVQNILGQYDKVHEKREEIVDQIKNINRETTQLKAKMQKLRDTCNQNTEKAQAIYDRLLTTLEQYHKRLENLVMEGSADIMKMKSNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PMW6</accession>
    <name>A0A2D0PMW6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NMDA receptor synaptonuclear signaling and neuronal migration factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">nsmfb</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017306761.1">
        <property type="nucleotide sequence ID" value="XM_017451272.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PMW6"/>
    <dbReference type="GeneID" id="108255351"/>
    <dbReference type="KEGG" id="ipu:108255351"/>
    <dbReference type="CTD" id="569891"/>
    <dbReference type="OMA" id="GRPCKSW"/>
    <dbReference type="OrthoDB" id="6161298at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001222">
        <property type="term" value="P:regulation of neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048168">
        <property type="term" value="P:regulation of neuronal synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033374">
        <property type="entry name" value="NSMF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32061">
        <property type="entry name" value="NMDA RECEPTOR SYNAPTONUCLEAR SIGNALING AND NEURONAL MIGRATION FACTOR"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
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        <location>
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        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
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            <begin position="168"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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        </location>
    </feature>
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        <location>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="218"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="338"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017306761.1"/>
        </source>
    </evidence>
    <sequence length="569" mass="65031" checksum="76B4AB3A91E5DB08" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PNL1</accession>
    <name>A0A2D0PNL1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein si:ch211-161h7.5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">si:ch211-161h7.5</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017308183.1">
        <property type="nucleotide sequence ID" value="XM_017452694.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PNL1"/>
    <dbReference type="GeneID" id="108256129"/>
    <dbReference type="KEGG" id="ipu:108256129"/>
    <dbReference type="OrthoDB" id="5586934at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33802">
        <property type="entry name" value="SI:CH211-161H7.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33802:SF4">
        <property type="entry name" value="SI:DKEY-29D8.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017308183.1"/>
        </source>
    </evidence>
    <sequence length="305" mass="34532" checksum="07327650A070E121" modified="2017-12-20" version="1">MGAHNIARIAIIVVSVVVYITGITFNILAAQGTGPFLMMTANVSDTYNTQITPSGWTFSIWGVIYFWLTAMFIYIVSTIFRRNAFGPMYCSRSVLPYGFFICWILNILFNISWLLLWDREVMIAALIMLAFVAFTNYMMIFFSCRGLKEYGAWLNKYHNKDLWCIRILVQNGLAVYTTWTSIATLINLTIVVSYDAGMSKSDAATLSLSLLSVEVLVWFVLENSFLEKHVRYILTVYPVIIVALSGNMTKNFDASAPGRNGIFIALLLALACVLFVTRVCLVIWRHKKHAHTQDVTSEMEIATKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PWC6</accession>
    <name>A0A2D0PWC6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4">Proteasome assembly chaperone 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">psmg2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Forms a heterodimer with PSMG1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the PSMG2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017309435.1">
        <property type="nucleotide sequence ID" value="XM_017453946.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PWC6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000015654"/>
    <dbReference type="GeneID" id="100528574"/>
    <dbReference type="CTD" id="56984"/>
    <dbReference type="OrthoDB" id="10260712at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10900:FF:000003">
        <property type="entry name" value="Proteasome assembly chaperone 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016562">
        <property type="entry name" value="Proteasome_assmbl_chp_2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12970">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12970:SF1">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010044">
        <property type="entry name" value="UCP010044"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159659">
        <property type="entry name" value="Cgl1923-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00019186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017309435.1"/>
        </source>
    </evidence>
    <sequence length="261" mass="28696" checksum="26F48B537BD19EFB" modified="2017-12-20" version="1">MFMSTGERAPSFTGFTLITPAVSVGNVGQLAADLIISTLDMVRVGHFHTDCLIPMAGNNPYSSGEEDAALLSTNAEVYCRGDLQLAVLQIRTPILPTKTKSFRKTLLSWIKTSGFSRTVLLSSCHAHHRDDQQLLTSPLRYLLTPALQGDTEGTLQRLGWREMERVSAFPGVTDSDRQRLYIPGGGVTKALYNSGCSENMSMAVLLLFCSEGDNIPDAFTLVNHLNDWLHLLDKPVQGSVPWRIPSSWRLLFGSGVPPMIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PX23</accession>
    <name>A0A2D0PX23_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Transcription factor Sp4 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">sp4</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the Sp1 C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017310589.1">
        <property type="nucleotide sequence ID" value="XM_017455100.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PX23"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005990"/>
    <dbReference type="GeneID" id="108257379"/>
    <dbReference type="KEGG" id="ipu:108257379"/>
    <dbReference type="CTD" id="6671"/>
    <dbReference type="OrthoDB" id="6365676at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035118">
        <property type="term" value="P:embryonic pectoral fin morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045743">
        <property type="term" value="P:positive regulation of fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22536">
        <property type="entry name" value="SP4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000014">
        <property type="entry name" value="Transcription factor Sp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000026">
        <property type="entry name" value="Transcription factor Sp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000061">
        <property type="entry name" value="Transcription factor Sp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF17">
        <property type="entry name" value="TRANSCRIPTION FACTOR SP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="606"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="636"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="666"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="336"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="559"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="722"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="347"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="559"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="722"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017310589.1"/>
        </source>
    </evidence>
    <sequence length="744" mass="78279" checksum="4BB2C145D820A308" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q0A2</accession>
    <name>A0A2D0Q0A2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transmembrane protein 54b isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tmem54b</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM54 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017310790.1">
        <property type="nucleotide sequence ID" value="XM_017455301.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q0A2"/>
    <dbReference type="GeneID" id="108257493"/>
    <dbReference type="KEGG" id="ipu:108257493"/>
    <dbReference type="CTD" id="557625"/>
    <dbReference type="OrthoDB" id="9389418at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020977">
        <property type="entry name" value="Beta-casein-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31258">
        <property type="entry name" value="KERATINOCYTE-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31258:SF5">
        <property type="entry name" value="TMEM54 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12304">
        <property type="entry name" value="BCLP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="264"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017310790.1"/>
        </source>
    </evidence>
    <sequence length="295" mass="32776" checksum="2B0F0C2FB1843D53" modified="2017-12-20" version="1">MPLSMVVVWCGASHVKLCVHFRRRLGGVSFTRPHEPMSLMKMGLCMVLVGHVNFLLAALVHGTVLRHITLDTQTLEYAISNILALTAGLVGILVGILTIVFSKNEKNRVLTWWVCMLSAVGGVLAAATVVGLLIALVWTFIYGTKGLLLHCNLPDDMSYFSITFECPFDPTRIYGTTLILWVPLIVMSVVEFVVSGRCCAVSVSFIRRRTRIHCETRPVCNTMVETLRAAVPPVIPCHVPPPPRCCHRDCELVRPPEQHELLRASRPYSSSRSAQNRAPSSPFNRASLNRASFWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q0M0</accession>
    <name>A0A2D0Q0M0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Macrophage-expressed gene 1 protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Perforin-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC108257561</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Pore-forming protein involved in both innate and adaptive immunity. Plays a central role in antigen cross-presentation in dendritic cells by forming a pore in antigen-containing compartments, thereby promoting delivery of antigens for cross-presentation. Also involved in innate immune response following bacterial infection; shows antibacterial activity against a wide spectrum of Gram-positive, Gram-negative and acid-fast bacteria. Reduces the viability of the intracytosolic pathogen L.monocytogenes by inhibiting acidification of the phagocytic vacuole of host cells which restricts bacterial translocation from the vacuole to the cytosol. Required for the antibacterial activity of reactive oxygen species and nitric oxide.</text>
    </comment>
    <comment type="function">
        <text evidence="17">Pore-forming protein that plays a central role in antigen cross-presentation in dendritic cells by mediating delivery of antigens for cross-presentation. Dendritic cells bridge innate and adaptive immunity by capturing exogenous antigens on MHC class-I molecules and presenting them to naive CD8(+) T-cells. Acts by forming a pore in antigen-containing compartments, promoting the release of antigens into the cytosol, enabling generation of MHCI:peptide complexes and T-cell priming.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Phagosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MPEG1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017310925.1">
        <property type="nucleotide sequence ID" value="XM_017455436.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q0M0"/>
    <dbReference type="GeneID" id="108257561"/>
    <dbReference type="KEGG" id="ipu:108257561"/>
    <dbReference type="OMA" id="YSERIDH"/>
    <dbReference type="OrthoDB" id="5950457at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22579">
        <property type="entry name" value="MPEG1_P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039707">
        <property type="entry name" value="MPEG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31463">
        <property type="entry name" value="MACROPHAGE-EXPRESSED GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31463:SF4">
        <property type="entry name" value="MACROPHAGE-EXPRESSED GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013243173" description="Macrophage-expressed gene 1 protein" evidence="20">
        <location>
            <begin position="21"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="645"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="MACPF" evidence="21">
        <location>
            <begin position="29"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00045689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017310925.1"/>
        </source>
    </evidence>
    <sequence length="708" mass="78722" checksum="660279A4572ECE24" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q109</accession>
    <name>A0A2D0Q109_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Malate dehydrogenase 1B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mdh1b</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017312418.1">
        <property type="nucleotide sequence ID" value="XM_017456929.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q109"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009324"/>
    <dbReference type="GeneID" id="108258355"/>
    <dbReference type="KEGG" id="ipu:108258355"/>
    <dbReference type="CTD" id="130752"/>
    <dbReference type="OrthoDB" id="1510206at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016615">
        <property type="term" value="F:malate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000144">
        <property type="entry name" value="Malate dehydrogenase [NADP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010945">
        <property type="entry name" value="Malate_DH_type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23382">
        <property type="entry name" value="MALATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="3">
        <location>
            <begin position="289"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017312418.1"/>
        </source>
    </evidence>
    <sequence length="477" mass="52873" checksum="29A8AD46E10DD099" modified="2017-12-20" version="1">MAKFVLAGKADCPYYAKAELLADLLQSILPDFCIHKICMLPSAWESWLEDTCSSNGWKHTSSPMVWRELTDRGGKGMLLGGFSDFLEYVQGYYGITSDMGTELMLNIAAENLRATELRLWDEERQRQLHQSFHIWISGALNPICNHLIPSLFTSGVFAAVPAVSLHLLDADASEDSLLALKMEVEDMALPQLHEVTVHSDLTVAFRAADLIVLLDERGPNEDAEQRLSRVVERLDCYGRLIEDNAHRDLRVLVAGDVYVNLKCGLLIERAPSVDPRRFVAIAAQLEGEARTQLAVQLSVKSHDITDVIVWGNISGRYHVDLQRAKVYRYKGAIWGPAGFSQNILEIVYDRKWLENDFPGSVSSRRSAISLKTNRPAAISATRGIITALSAWINDSSPQEIFSLGVVSTGHFGIPVGLVFCVPVTFEKGEWSVCADVVVTEELRRKLEAAVNEITAEKSTADGMRTKSSLTSYSNSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q7T4</accession>
    <name>A0A2D0Q7T4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Protein CBFA2T3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">cbfa2t3</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017314748.2">
        <property type="nucleotide sequence ID" value="XM_017459259.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q7T4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035117"/>
    <dbReference type="GeneID" id="108259624"/>
    <dbReference type="KEGG" id="ipu:108259624"/>
    <dbReference type="CTD" id="863"/>
    <dbReference type="OrthoDB" id="8872930at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.140.2220:FF:000001">
        <property type="entry name" value="CBFA2/RUNX1 translocation partner 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1110:FF:000001">
        <property type="entry name" value="RUNX1 translocation partner 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1110">
        <property type="entry name" value="TAFH/NHR1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013289">
        <property type="entry name" value="CBFA2T1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014896">
        <property type="entry name" value="NHR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037249">
        <property type="entry name" value="TAFH/NHR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003894">
        <property type="entry name" value="TAFH_NHR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10379">
        <property type="entry name" value="MTG8 ETO EIGHT TWENTY ONE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10379:SF6">
        <property type="entry name" value="PROTEIN CBFA2T3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08788">
        <property type="entry name" value="NHR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07531">
        <property type="entry name" value="TAFH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01875">
        <property type="entry name" value="ETOFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00549">
        <property type="entry name" value="TAFH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158553">
        <property type="entry name" value="TAFH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51119">
        <property type="entry name" value="TAFH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TAFH" evidence="13">
        <location>
            <begin position="163"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="MYND-type" evidence="12">
        <location>
            <begin position="564"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="46"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="310"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="370"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="610"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="503"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="106"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="313"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="619"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="661"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017314748.2"/>
        </source>
    </evidence>
    <sequence length="688" mass="75271" checksum="E394ABFA36893B67" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q7Z6</accession>
    <name>A0A2D0Q7Z6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Extracellular serine/threonine protein kinase FAM20C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">fam20cl</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM20 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017314424.1">
        <property type="nucleotide sequence ID" value="XM_017458935.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q7Z6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019837"/>
    <dbReference type="GeneID" id="108259419"/>
    <dbReference type="KEGG" id="ipu:108259419"/>
    <dbReference type="CTD" id="100007251"/>
    <dbReference type="OrthoDB" id="8583677at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070166">
        <property type="term" value="P:enamel mineralization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10314">
        <property type="entry name" value="FAM20_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024869">
        <property type="entry name" value="FAM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009581">
        <property type="entry name" value="FAM20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12450">
        <property type="entry name" value="DENTIN MATRIX PROTEIN 4 PROTEIN FAM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12450:SF25">
        <property type="entry name" value="FAM20 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06702">
        <property type="entry name" value="Fam20C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="FAM20 C-terminal" evidence="11">
        <location>
            <begin position="266"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="40"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="371"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="302"/>
            <end position="305"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="376"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017314424.1"/>
        </source>
    </evidence>
    <sequence length="496" mass="56650" checksum="0E8F2CC0E06766CD" modified="2017-12-20" version="1">MRVRQCRSLKGVCVMLVCVMLALHVLVILVTLSTFPSTCEPIQRSQTEPRNLTSANLTHSGSTSTARKRAADLAKLEALFSHALYSLHAPSSPDDDTLLRIRTQEEHSEEHSAQQWLSNSKDGYEPVQWNSSTDTHPPWLRFHLGISRWQLYPHRDTNLPVLVEQLSMHRIVSAVQKTGGTQLKLVMTFSNYGEALFKPMKQERYDETNVNLYYFSDFERHNAEIAAFHLDRILGFRHVPPVVGRLVDVIKEIKDITTDHKLAKTFFSSPVGNVCFYGQCSYYCSTEHAVCGRPRALEGSVAAMLPDLALAPRRSWRNPWRRSYSRSKLALWEKTPGYCDAVKKTPPYDRGTRLVDLIDMTILDFLQSNMDRHHYETFEIFGNNTFLLHLDNGRAFGRHSKDEPSILTPLVQCCRIRRSTLLRLRLLSSPQYRLSDVMRASLSQDPLAAVAPLLTEPHLSALDRRLAAVLQTVQRCLLQHQHLHDVIYDDITDYSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q8P0</accession>
    <name>A0A2D0Q8P0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Sal-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">sall1a</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Transcriptional repressor involved in organogenesis. Plays an essential role in ureteric bud invasion during kidney development.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">May associate with NuRD histone deacetylase complex (HDAC). Interacts with components of HDAC complex including HDAC1, HDAC2, RBBP4, RBPP7, MTA1 and MTA2. Interacts with CCNQ. Interacts with NSD2 (via PHD-type zinc fingers 1, 2 and 3).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the sal C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017314669.2">
        <property type="nucleotide sequence ID" value="XM_017459180.3"/>
    </dbReference>
    <dbReference type="GeneID" id="108259583"/>
    <dbReference type="KEGG" id="ipu:108259583"/>
    <dbReference type="CTD" id="555721"/>
    <dbReference type="OrthoDB" id="8749569at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003337">
        <property type="term" value="P:mesenchymal to epithelial transition involved in metanephros morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021772">
        <property type="term" value="P:olfactory bulb development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035295">
        <property type="term" value="P:tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20908">
        <property type="entry name" value="SUF4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000708">
        <property type="entry name" value="Sal-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000689">
        <property type="entry name" value="Spalt like transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000025">
        <property type="entry name" value="Spalt-like transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000260">
        <property type="entry name" value="Spalt-like transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000302">
        <property type="entry name" value="Spalt-like transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000341">
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            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
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                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
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                <person name="Wang X."/>
                <person name="Chen A."/>
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                <person name="Yuan Z."/>
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                <person name="Peatman E."/>
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                <person name="Kucuktas H."/>
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                <person name="Liu H."/>
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                <person name="Kirby M.L."/>
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    <sequence length="309" mass="35527" checksum="A67DA61F1791919E" modified="2017-12-20" version="1">MEMHNDTSNTLVFNGNVSVPEKTVYEMCSHMPEIILFYLGMQIINMFMGIPTNLIVLWLIHRNRSDSSTSDIFIWHLAVLDTFFCLIPPLELANLLYLTTSSTWYILRFFYGIKDMSPLFLACICMDRYMAVCHPIVFSNLKDQRHRPVCAVVVWFITLVYAILKCLGNIPNFDKVFTVMILATFAFMLFCNISILWALSQSGQGRDDMHPVKKRAFKMVLIILAIIVFNYLPPVALFPFQPYFSPDVFKCYIHYIAFGFMDISSSIQPVLYLSNKKLQCPAWCCECCSNKDTETGTRPESSTVYTTNA</sequence>
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            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Bao L."/>
                <person name="Zhang J."/>
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                <person name="Jiang C."/>
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                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017314755.1">
        <property type="nucleotide sequence ID" value="XM_017459266.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QBJ1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034438"/>
    <dbReference type="GeneID" id="108259626"/>
    <dbReference type="KEGG" id="ipu:108259626"/>
    <dbReference type="CTD" id="8854"/>
    <dbReference type="OMA" id="WSNTFNK"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07141">
        <property type="entry name" value="ALDH_F1AB_F2_RALDH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000050">
        <property type="entry name" value="Aldehyde dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000026">
        <property type="entry name" value="Aldehyde dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000001">
        <property type="entry name" value="Mitochondrial aldehyde dehydrogenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="6">
        <location>
            <begin position="46"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017314755.1"/>
        </source>
    </evidence>
    <sequence length="518" mass="56440" checksum="DFA30733E23BABB4" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QBK1</accession>
    <name>A0A2D0QBK1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Transcription factor HES-7.1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">her7</name>
        <name evidence="11" type="synonym">LOC128631199</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017316068.1">
        <property type="nucleotide sequence ID" value="XM_017460579.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053535251.1">
        <property type="nucleotide sequence ID" value="XM_053679276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QBK1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032481"/>
    <dbReference type="GeneID" id="108260346"/>
    <dbReference type="KEGG" id="ipu:108260346"/>
    <dbReference type="KEGG" id="ipu:128631199"/>
    <dbReference type="CTD" id="58132"/>
    <dbReference type="GeneTree" id="ENSGT00700000104815"/>
    <dbReference type="OMA" id="LGCHANT"/>
    <dbReference type="OrthoDB" id="8930573at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="13"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="8">
        <location>
            <begin position="92"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="162"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="180"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017316068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053535251.1"/>
        </source>
    </evidence>
    <sequence length="205" mass="23782" checksum="A439A5CB296643C3" modified="2017-12-20" version="1">MNKFEETERLKIDRKLLKPQVERRRRERMNRSLENLRILLLQGPEQQAMSQRRVEKAEILEHTVLFLQSSITEAKKSRPEGPDESSPEGHQFRDGFSACLQKAARFLQEESEARGLHNSLSSSLYHCLSRPHWHSARDARQCHGTQGLQCVRRHTHTLSHPYRIPPQHTDPNTAHRHHYQNTAGTAQPTASPQAAGRQCVWRPWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QBV2</accession>
    <name>A0A2D0QBV2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RPA-related protein RADX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">radx</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017315186.1">
        <property type="nucleotide sequence ID" value="XM_017459697.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QBV2"/>
    <dbReference type="GeneID" id="108259901"/>
    <dbReference type="KEGG" id="ipu:108259901"/>
    <dbReference type="CTD" id="55086"/>
    <dbReference type="OMA" id="TGCHKGQ"/>
    <dbReference type="OrthoDB" id="123282at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040893">
        <property type="entry name" value="RADX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14944">
        <property type="entry name" value="RPA-RELATED PROTEIN RADX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14944:SF4">
        <property type="entry name" value="RPA1 RELATED SINGLE STRANDED DNA BINDING PROTEIN, X-LINKED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17659">
        <property type="entry name" value="RADX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="544"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="621"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="564"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="574"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="621"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="645"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017315186.1"/>
        </source>
    </evidence>
    <sequence length="850" mass="95126" checksum="2B7CA93CEF39CDA5" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QD62</accession>
    <name>A0A2D0QD62_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Junctional adhesion molecule A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Junctional adhesion molecule 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">f11r.1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="18">Interacts with the ninth PDZ domain of MPDZ. Interacts with the first PDZ domain of PARD3. The association between PARD3 and PARD6B probably disrupts this interaction. Interacts with ITGAL (via I-domain). Interacts with CD151.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the immunoglobulin superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017315310.1">
        <property type="nucleotide sequence ID" value="XM_017459821.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QD62"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000014073"/>
    <dbReference type="GeneID" id="108259963"/>
    <dbReference type="KEGG" id="ipu:108259963"/>
    <dbReference type="CTD" id="323696"/>
    <dbReference type="OMA" id="VEWKFVH"/>
    <dbReference type="OrthoDB" id="10031887at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090557">
        <property type="term" value="P:establishment of endothelial intestinal barrier"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050892">
        <property type="term" value="P:intestinal absorption"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090559">
        <property type="term" value="P:regulation of membrane permeability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000342">
        <property type="entry name" value="Junctional adhesion molecule A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042456">
        <property type="entry name" value="F11R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45113">
        <property type="entry name" value="JUNCTIONAL ADHESION MOLECULE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45113:SF1">
        <property type="entry name" value="JUNCTIONAL ADHESION MOLECULE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 21" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0796">Tight junction</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012813282" description="Junctional adhesion molecule A" evidence="21">
        <location>
            <begin position="18"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="22">
        <location>
            <begin position="16"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="22">
        <location>
            <begin position="122"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="262"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00030590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00046718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_017315310.1"/>
        </source>
    </evidence>
    <sequence length="292" mass="31914" checksum="88D44B4074CE047D" modified="2017-12-20" version="1">MFLVFVLLCWQATGLHAFSASSSTPTVRVPENSGADLKCDYSADFGATPRVEWKFSSITGSQSFIYFDGQITDKYKGRMTQYQGGLRIEKATRQDTGDYSCEVSGNSGYAEVKIKLTVLVPPSVPISRIPTSATTGRSVLLTCFDKDGSPPSTYKWYKNGTPLPDDPSKFPNFKNLTYKLNSLNGNLEFPSVSKADSGSYYCEASNGQGPPQRGPAVVMEVRDLNTGGIVAGVLVALLAIALLCFAVWFANKKGYLPKMSESKPKTAVYTQPRQDYGDEDDGEFKQKQSFVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QEM9</accession>
    <name>A0A2D0QEM9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 18">Vesicle-fusing ATPase</fullName>
            <ecNumber evidence="3 18">3.6.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC108260232</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="18">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Homohexamer. Interacts with GABARAP and GABARAPL2. Interacts with GRIA2. Interacts with PLK2, leading to disrupt the interaction with GRIA2. Interacts with MUSK; may regulate MUSK endocytosis and activity. Interacts with CDK16.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.6" evidence="3 18"/>
    <dbReference type="RefSeq" id="XP_017315815.1">
        <property type="nucleotide sequence ID" value="XM_017460326.3"/>
    </dbReference>
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    <dbReference type="KEGG" id="ipu:108260232"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035494">
        <property type="term" value="P:SNARE complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19504">
        <property type="entry name" value="RecA-like_NSF-SEC18_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000026">
        <property type="entry name" value="vesicle-fusing ATPase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000031">
        <property type="entry name" value="vesicle-fusing ATPase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="vesicle-fusing ATPase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="vesicle-fusing ATPase isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="vesicle-fusing ATPase isoform X1"/>
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        <property type="entry name" value="Vesicular-fusion ATPase SEC18"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054419">
        <property type="entry name" value="NSF_ATPase_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039812">
        <property type="entry name" value="Vesicle-fus_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23078:SF3">
        <property type="entry name" value="VESICLE-FUSING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23078">
        <property type="entry name" value="VESICULAR-FUSION PROTEIN NSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21964">
        <property type="entry name" value="NSF_ATPase_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 18" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="11 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14 18" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 18" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="21">
        <location>
            <begin position="30"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="20">
        <location>
            <begin position="136"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="19">
        <location>
            <begin position="277"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="19">
        <location>
            <begin position="560"/>
            <end position="696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU367045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017315815.1"/>
        </source>
    </evidence>
    <sequence length="772" mass="85161" checksum="B687C3ADC6B55091" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QFY2</accession>
    <name>A0A2D0QFY2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">5,6-dihydroxyindole-2-carboxylic acid oxidase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Tyrosinase-related protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">LOC108260967</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">SDA103</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="27">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>2 5,6-dihydroxyindole-2-carboxylate + O2 = 2 indole-5,6-quinone-2-carboxylate + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:68388"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16875"/>
            <dbReference type="ChEBI" id="CHEBI:177869"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:68389"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="17">Pigment biosynthesis; melanin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Melanosome membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the tyrosinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017317214.1">
        <property type="nucleotide sequence ID" value="XM_017461725.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QFY2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000017354"/>
    <dbReference type="GeneID" id="108260967"/>
    <dbReference type="KEGG" id="ipu:108260967"/>
    <dbReference type="OMA" id="WPRRFFT"/>
    <dbReference type="OrthoDB" id="6132182at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033162">
        <property type="term" value="C:melanosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042438">
        <property type="term" value="P:melanin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030318">
        <property type="term" value="P:melanocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032438">
        <property type="term" value="P:melanosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048023">
        <property type="term" value="P:positive regulation of melanin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1280.10:FF:000001">
        <property type="entry name" value="5,6-dihydroxyindole-2-carboxylic acid oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1280.10">
        <property type="entry name" value="Di-copper center containing domain from catechol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008922">
        <property type="entry name" value="Di-copper_centre_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050316">
        <property type="entry name" value="Tyrosinase/Hemocyanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002227">
        <property type="entry name" value="Tyrosinase_Cu-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11474:SF3">
        <property type="entry name" value="5,6-DIHYDROXYINDOLE-2-CARBOXYLIC ACID OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11474">
        <property type="entry name" value="TYROSINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00264">
        <property type="entry name" value="Tyrosinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00092">
        <property type="entry name" value="TYROSINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48056">
        <property type="entry name" value="Di-copper centre-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00497">
        <property type="entry name" value="TYROSINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00498">
        <property type="entry name" value="TYROSINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0186">Copper</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0470">Melanin biosynthesis</keyword>
    <keyword evidence="14 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 23" id="KW-0732">Signal</keyword>
    <keyword evidence="5 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012632716" description="5,6-dihydroxyindole-2-carboxylic acid oxidase" evidence="23">
        <location>
            <begin position="19"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="468"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosinase copper-binding" evidence="24">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosinase copper-binding" evidence="25">
        <location>
            <begin position="388"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="500"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="500"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00037907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00040647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00041445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS00498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_017317214.1"/>
        </source>
    </evidence>
    <sequence length="526" mass="59077" checksum="4600965093B068CE" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QM08</accession>
    <name>A0A2D0QM08_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Polyadenylate-binding protein 1-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">LOC108263279</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7 8">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017319423.1">
        <property type="nucleotide sequence ID" value="XM_017463934.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017319432.1">
        <property type="nucleotide sequence ID" value="XM_017463943.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QM08"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029435"/>
    <dbReference type="GeneID" id="108263279"/>
    <dbReference type="KEGG" id="ipu:108263279"/>
    <dbReference type="GeneTree" id="ENSGT00940000153773"/>
    <dbReference type="OrthoDB" id="19742at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12379">
        <property type="entry name" value="RRM2_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045305">
        <property type="entry name" value="RRM2_I_PABPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="3"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="89"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="189"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="266"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017319423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017319432.1"/>
        </source>
    </evidence>
    <sequence length="488" mass="53458" checksum="CC13462C56826048" modified="2017-12-20" version="1">MATTLYVGDLHPDVTESMLLEKFSSVGRVCYLCLCLSTSTSFQHAFIIFQHRTDAERALKLLNFDILLGQPMRIMWSQSDSSHRNSYEGNIFIKGLEKSIDSMALYDTFSTFGNILSCKVVCDENGSRGFGYVHFESAEAAEAAIKRLNGMLFNDHYVFITHFKSRQELQEQKKEERHPKPQHKFEQCVNLCLRNLDVSVDDERLHKEFSPFGTIIHAKVMGTDGCSGGFGFVRFSSPEEATKAMSEMQGRMLDRRALHIRLIQRKKERQTSLSNQGTQAAPKAVVNHHHPAPSCDKIMDAIPQAQNSTDNSTSVHQVKPHPSTHKFTQDFQLQSFRNIPEVIAPAMTHPEANQVVAMAKETQTIVMDTVPAVVSVAAPTAAKAVLPTILAGSIKAVPAVVPAAGWKPIPTASIKAVPAAVPAAGWKPIPTVSIKAVPAVVPAAGWKSIPTASIKAVPAAATPAPVIDEQLTKDTQQSQDTQVEHCFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QMP2</accession>
    <name>A0A2D0QMP2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">MARCKS-related protein 1-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">marcksl1a</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MARCKS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017319564.1">
        <property type="nucleotide sequence ID" value="XM_017464075.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QMP2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032667"/>
    <dbReference type="GeneID" id="108263363"/>
    <dbReference type="KEGG" id="ipu:108263363"/>
    <dbReference type="CTD" id="406407"/>
    <dbReference type="OMA" id="HVIYNGD"/>
    <dbReference type="OrthoDB" id="9948538at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002101">
        <property type="entry name" value="MARCKS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14353:SF8">
        <property type="entry name" value="MARCKS-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14353">
        <property type="entry name" value="MYRISTOYLATED ALANINE-RICH C-KINASE SUBSTRATE MARCKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02063">
        <property type="entry name" value="MARCKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00963">
        <property type="entry name" value="MARCKS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00826">
        <property type="entry name" value="MARCKS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0519">Myristate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="102"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="195"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017319564.1"/>
        </source>
    </evidence>
    <sequence length="219" mass="22092" checksum="9F8547F4B40A1A6A" modified="2017-12-20" version="1">MGAQLSKGGAAEAESAAAKTNGQENGHVKTNGDVSAKPEGEAAVADGNGTTEAVKETAESGDAIEAADGEGTSANAAEGEAPKETKKKKKFSLKNSFKFKGFSLKKNKKSTEEPAEAAAAAPEENGQAPKETKEEEEPAAETNEAAAEAEAEAEVEAEAEAAPAPEAAEEEAKAEEPAPVAEATPTEESAKTEEAPASTPAETEATPEATPAEPTPASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QPE1</accession>
    <name>A0A2D0QPE1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Decaprenyl-diphosphate synthase subunit 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">pdss2</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3 4">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017319590.2">
        <property type="nucleotide sequence ID" value="XM_017464101.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053535285.1">
        <property type="nucleotide sequence ID" value="XM_053679310.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053535288.1">
        <property type="nucleotide sequence ID" value="XM_053679313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QPE1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003080"/>
    <dbReference type="GeneID" id="108263381"/>
    <dbReference type="KEGG" id="ipu:108263381"/>
    <dbReference type="CTD" id="57107"/>
    <dbReference type="OrthoDB" id="9983019at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF55">
        <property type="entry name" value="ALL TRANS-POLYPRENYL-DIPHOSPHATE SYNTHASE PDSS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053535285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053535288.1"/>
        </source>
    </evidence>
    <sequence length="381" mass="41324" checksum="6AB8BD249F522B27" modified="2017-12-20" version="1">MAVRSRLALLTSLGSPRRGRRSLSLFSGPSPSGWSKVVSDAEKIVGYPTSFMSLRCLLSDELSNVAMHVRKLVGTKHPLLNTARGFVYDSKHNLQMRGLVVLLMSKAAGRSESASGALLPDMISGIYPSQRSLAEITELIHTAFLVHRGIVNLKEWTFSDGPLKDMQFGNKMAVLSGDFLLANACTGLAQLNNTKVVELISSAIGDLVQGVYYENSNSNSAEGKLSMASWEDQAFLSHGALLAKSCQAAMELARHDAEAQTLAYEYGKHLALAHKLNLDLQPFMKSGSDPVVFSLDSAPAVFHRLIVGHEIWTRQLQQAQISTPQTDYSKLWSGIRSGGGVKQAVDLCTEHGNTALEAIRRFPPSEARSALENIAGAVARF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QUK9</accession>
    <name>A0A2D0QUK9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Liprin-beta-1b isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ppfibp1b</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May regulate the disassembly of focal adhesions. Did not bind receptor-like tyrosine phosphatases type 2A.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the liprin family. Liprin-beta subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017322108.1">
        <property type="nucleotide sequence ID" value="XM_017466619.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QUK9"/>
    <dbReference type="GeneID" id="108264769"/>
    <dbReference type="KEGG" id="ipu:108264769"/>
    <dbReference type="CTD" id="561961"/>
    <dbReference type="OrthoDB" id="6516566at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048786">
        <property type="term" value="C:presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007528">
        <property type="term" value="P:neuromuscular junction development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09563">
        <property type="entry name" value="SAM_liprin-beta1_2_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09566">
        <property type="entry name" value="SAM_liprin-beta1_2_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09569">
        <property type="entry name" value="SAM_liprin-beta1_2_repeat3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000005">
        <property type="entry name" value="Liprin-beta-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000007">
        <property type="entry name" value="Liprin-beta-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000017">
        <property type="entry name" value="Liprin-beta-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058914">
        <property type="entry name" value="LIPB1/2_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037617">
        <property type="entry name" value="LIPB1/2_SAM_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037618">
        <property type="entry name" value="LIPB1/2_SAM_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037619">
        <property type="entry name" value="LIPB1/2_SAM_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029515">
        <property type="entry name" value="Liprin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12587">
        <property type="entry name" value="LAR INTERACTING PROTEIN LIP -RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12587:SF16">
        <property type="entry name" value="LIPRIN-BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26022">
        <property type="entry name" value="CC_Liprin_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SAM" evidence="8">
        <location>
            <begin position="647"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="8">
        <location>
            <begin position="719"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="280"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="352"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="436"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="557"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="317"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="299"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="360"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="437"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="464"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="566"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017322108.1"/>
        </source>
    </evidence>
    <sequence length="1009" mass="113972" checksum="124AABB6319E50B9" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QWF8</accession>
    <name>A0A2D0QWF8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">renin</fullName>
            <ecNumber evidence="4">3.4.23.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Angiotensinogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ren</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of Leu-|-Xaa bond in angiotensinogen to generate angiotensin I.</text>
            <dbReference type="EC" id="3.4.23.15"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.15" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017322757.1">
        <property type="nucleotide sequence ID" value="XM_017467268.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QWF8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000017925"/>
    <dbReference type="GeneID" id="108265134"/>
    <dbReference type="KEGG" id="ipu:108265134"/>
    <dbReference type="CTD" id="5972"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002003">
        <property type="term" value="P:angiotensin maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000042">
        <property type="entry name" value="Cathepsin D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000032">
        <property type="entry name" value="renin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012848">
        <property type="entry name" value="Aspartic_peptidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF24">
        <property type="entry name" value="RENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07966">
        <property type="entry name" value="A1_Propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="11 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 15" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 16" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013016961" description="renin" evidence="16">
        <location>
            <begin position="17"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="17">
        <location>
            <begin position="73"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="104"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="268"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="311"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017322757.1"/>
        </source>
    </evidence>
    <sequence length="392" mass="42748" checksum="F6A3C82D3A53A288" modified="2017-12-20" version="1">MKAWALLLSLCVTAQALRRITLKKMPSIRETLKETGVTPAEVFAELAPKLDASPSLHNGTATTPLTNYLDTQYFGEISIGTPAQMFNVVFDTGSANLWVPSQSCSPLYTACFIHNRYDASKSHTHIQNGTGFSIQYASGNVRGFLSEDIVVVSGIPVVQVFAEATALPAIPFIFAKFDGVLGMGYPEAAIDGITPVFDRIMSQGVLKEDIFSVYYSRDPEHIPGGELVLGGTDPNYYTGLFHYISTKGEGKWEVIMKGVSVGKDTLFCTEGCNTMIDTGSSYITGPASSVSILMKTIGAVELAEGGYTVNCDLVMSLPSVTFHLGDQEYPLTQEDYVLWQSQFGEDICTVTFRELDLPPPAGPVWILGAKFISRYYTVFDRKNNRIGFAHAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QYK6</accession>
    <name>A0A2D0QYK6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Par-6 family cell polarity regulator gamma a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">pard6ga</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PAR6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017322836.2">
        <property type="nucleotide sequence ID" value="XM_017467347.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QYK6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003116"/>
    <dbReference type="GeneID" id="108265222"/>
    <dbReference type="KEGG" id="ipu:108265222"/>
    <dbReference type="CTD" id="100170824"/>
    <dbReference type="OrthoDB" id="5868434at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007098">
        <property type="term" value="P:centrosome cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060341">
        <property type="term" value="P:regulation of cellular localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06403">
        <property type="entry name" value="PB1_Par6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06718">
        <property type="entry name" value="PDZ_Par6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000031">
        <property type="entry name" value="Partitioning defective 6 homolog alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000030">
        <property type="entry name" value="Partitioning defective 6 homolog beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051741">
        <property type="entry name" value="PAR6_homolog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034868">
        <property type="entry name" value="PB1_Par6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14102">
        <property type="entry name" value="PAR-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14102:SF3">
        <property type="entry name" value="PARTITIONING DEFECTIVE 6 HOMOLOG GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0796">Tight junction</keyword>
    <feature type="domain" description="PB1" evidence="14">
        <location>
            <begin position="57"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="13">
        <location>
            <begin position="196"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="350"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="351"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017322836.2"/>
        </source>
    </evidence>
    <sequence length="453" mass="50646" checksum="799DF1E48543E108" modified="2023-02-22" version="2">MMPSQSFTRSSSAHEHCIINCRHSLVTLTKQNLPGPDYKGKMIRSLGKPSAWRCRSAVEVKSKFGAEFRRFSLDRYEPGVFKDFCQFILKLHHLFPAEIYISYADVHGDLLPINNDDNFCKAVSTAQSLLRVFIQLQEEMDQVNICTNCVTRRKKSTIMVHGDASQRGHVQIGLPQNFRPVSSIIDVDILPECHRRVRLYHQGSDRPLGFYIRDGITFSVTPHGLEKVPGIFISRLVPGGLAESCGLLAINDQILEVNGIEVIGKTLDQVTDMMIANSRNLIITVKPANQSKHIKSPAVSPNPELQFDGKGSISYPGLPMIMGAYVWTHNDSVSDDDSDMVVEKPLKPSAHFSSTTVSSPSFQTSIHNRHRHSRPYSQESSRSQLYLNYTAHPGFSTSLQREISSQQIYMSNPAFRQSTSSPFDILGSLSPALRQRLMIPQGSMEEDGVVITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R211</accession>
    <name>A0A2D0R211_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">[histone H3]-lysine(4) N-trimethyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.354</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">setdb1b</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Plays a role in promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes. Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60260"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.354"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.354" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017324604.1">
        <property type="nucleotide sequence ID" value="XM_017469115.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R211"/>
    <dbReference type="GeneID" id="108266106"/>
    <dbReference type="KEGG" id="ipu:108266106"/>
    <dbReference type="CTD" id="768131"/>
    <dbReference type="OrthoDB" id="308383at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140999">
        <property type="term" value="F:histone H3K4 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046974">
        <property type="term" value="F:histone H3K9 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006346">
        <property type="term" value="P:DNA methylation-dependent constitutive heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070828">
        <property type="term" value="P:heterochromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01395">
        <property type="entry name" value="HMT_MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10517">
        <property type="entry name" value="SET_SETDB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20382">
        <property type="entry name" value="Tudor_SETDB1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21181">
        <property type="entry name" value="Tudor_SETDB1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000017">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000020">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000034">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000037">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040880">
        <property type="entry name" value="DUF5604"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025796">
        <property type="entry name" value="Hist-Lys_N-MeTrfase_SETDB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001739">
        <property type="entry name" value="Methyl_CpG_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007728">
        <property type="entry name" value="Pre-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047232">
        <property type="entry name" value="SETDB1/2-like_MBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051516">
        <property type="entry name" value="SETDB_methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041292">
        <property type="entry name" value="Tudor_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041291">
        <property type="entry name" value="TUDOR_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46024">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE EGGLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46024:SF2">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETDB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18300">
        <property type="entry name" value="DUF5604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01429">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05033">
        <property type="entry name" value="Pre-SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18358">
        <property type="entry name" value="Tudor_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18359">
        <property type="entry name" value="Tudor_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00391">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00468">
        <property type="entry name" value="PreSET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50867">
        <property type="entry name" value="PRE_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51573">
        <property type="entry name" value="SAM_MT43_SUVAR39_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Pre-SET" evidence="21">
        <location>
            <begin position="762"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="20">
        <location>
            <begin position="838"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="22">
        <location>
            <begin position="1221"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="99"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="483"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="906"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="991"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="493"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="936"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="950"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="998"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1037"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1054"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1080"/>
            <end position="1089"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00055955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_017324604.1"/>
        </source>
    </evidence>
    <sequence length="1237" mass="137867" checksum="B894F30B324B4279" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R4U2</accession>
    <name>A0A2D0R4U2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4 5">Formiminotransferase N-terminal subdomain-containing protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4 5" type="primary">ftcdnl1</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3 4 5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3 4 5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017325065.1">
        <property type="nucleotide sequence ID" value="XM_017469576.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017325066.1">
        <property type="nucleotide sequence ID" value="XM_017469577.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017325067.1">
        <property type="nucleotide sequence ID" value="XM_017469578.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R4U2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006072"/>
    <dbReference type="GeneID" id="108266379"/>
    <dbReference type="KEGG" id="ipu:108266379"/>
    <dbReference type="CTD" id="348751"/>
    <dbReference type="GeneTree" id="ENSGT00390000005581"/>
    <dbReference type="OMA" id="TAPHREN"/>
    <dbReference type="OrthoDB" id="48036at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005542">
        <property type="term" value="F:folic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.670">
        <property type="entry name" value="Formiminotransferase, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.990.10">
        <property type="entry name" value="Formiminotransferase, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037070">
        <property type="entry name" value="Formiminotransferase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012886">
        <property type="entry name" value="Formiminotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037064">
        <property type="entry name" value="Formiminotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022384">
        <property type="entry name" value="FormiminoTrfase_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051623">
        <property type="entry name" value="FTCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12234:SF1">
        <property type="entry name" value="FORMIMINOTRANSFERASE N-TERMINAL SUBDOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12234">
        <property type="entry name" value="FORMIMINOTRANSFERASE-CYCLODEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07837">
        <property type="entry name" value="FTCD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01222">
        <property type="entry name" value="FTCD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55116">
        <property type="entry name" value="Formiminotransferase domain of formiminotransferase-cyclodeaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Formiminotransferase N-terminal subdomain" evidence="1">
        <location>
            <begin position="8"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017325065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017325066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017325067.1"/>
        </source>
    </evidence>
    <sequence length="325" mass="34941" checksum="9B1E59AC7C74BF8A" modified="2017-12-20" version="1">MTSALGRRLVACLLNISEARERDVVEKVAQAAISHQHGGRRKGVTVLNIFSDHDYNRSVLTIVAGIELIGEVVLSASERAFSLIDMTVHEGIHPCMGAVDLVPFYPLGEEVSLEDCGKEARAVGTALTERVPGTSAFFFSWADIPLHRGLAHRRKELGWFRKGSNTATVRPDVGPPPIRRYGLTGIGASPYVMNCNVTIDTQDVSLGRDVAAAIRESSPGGIPGVQVMALPHEGTVEIACNVESVPAPAVGGESWPAFRVGEQKFCHAPASLITTRVAELARCQGVTTQGAALVGFTPLECKRLAERALSQDIGEFWKELHGVRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R5B0</accession>
    <name>A0A2D0R5B0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SPRY domain-containing protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">spryd7b</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017325759.1">
        <property type="nucleotide sequence ID" value="XM_017470270.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R5B0"/>
    <dbReference type="GeneID" id="108266652"/>
    <dbReference type="KEGG" id="ipu:108266652"/>
    <dbReference type="CTD" id="393498"/>
    <dbReference type="OMA" id="HMGNEVV"/>
    <dbReference type="OrthoDB" id="40953at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="CDD" id="cd12880">
        <property type="entry name" value="SPRYD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035766">
        <property type="entry name" value="SPRYD7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20951">
        <property type="entry name" value="C13ORF1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20951:SF2">
        <property type="entry name" value="SPRY DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="2">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00021772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017325759.1"/>
        </source>
    </evidence>
    <sequence length="197" mass="21650" checksum="A767BCBF0135CF91" modified="2017-12-20" version="1">MAAMFTCCLGYCCGDGGSDHKPLKEMPTVQLDTHHMGADVVVVKRGRRICGSGACLANAPLHQNKSYFEFKVQSTGVWGVGVATQKVNLNQIPLGQDSHSLVLRHDGSVYHNNEEKNCLPANSVPQEGDIVGLTYDHVELNLYLNGKNMQCPASGIRGTVFPVVYVDDSAILDCQFSDFYHTPPQGFEKILFEQQIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R5W2</accession>
    <name>A0A2D0R5W2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Neurogenic differentiation factor 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC108266782</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017325997.1">
        <property type="nucleotide sequence ID" value="XM_017470508.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R5W2"/>
    <dbReference type="GeneID" id="108266782"/>
    <dbReference type="KEGG" id="ipu:108266782"/>
    <dbReference type="OrthoDB" id="6125763at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11416">
        <property type="entry name" value="bHLH_TS_ceHLH13_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000035">
        <property type="entry name" value="Pancreas-specific transcription factor 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050283">
        <property type="entry name" value="E-box_TF_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349:SF97">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="120"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="69"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017325997.1"/>
        </source>
    </evidence>
    <sequence length="217" mass="25219" checksum="CBC6EF7B4B5CF9D5" modified="2017-12-20" version="1">MEDMLFDFDQDATTDFAFWGEMDPNFQFQSQLDSLLFDGTAITGELSPWSSFGCQSVFPEAQLAFPELESRSPRAEADSGAAEEELEEQKRGKLRPRRLASNHLAHHQHHHQHQHHPYRVQRHAANIRERKRMLSINSAFEELRCHVPTFPYEKRLSKIDTLRLAIAYIALLREILVSGCDPKTYVDECMKNGYKNQTNAIWNTSDLTARLSWIKWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R765</accession>
    <name>A0A2D0R765_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Frizzled-5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">fzd5</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor Fz/Smo family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017325896.1">
        <property type="nucleotide sequence ID" value="XM_017470407.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R765"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035383"/>
    <dbReference type="GeneID" id="108266738"/>
    <dbReference type="KEGG" id="ipu:108266738"/>
    <dbReference type="CTD" id="7855"/>
    <dbReference type="OMA" id="QLNCAQP"/>
    <dbReference type="OrthoDB" id="10053709at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042813">
        <property type="term" value="F:Wnt receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035567">
        <property type="term" value="P:non-canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07456">
        <property type="entry name" value="CRD_FZ5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2000.10:FF:000004">
        <property type="entry name" value="Frizzled class receptor 8a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000053">
        <property type="entry name" value="Frizzled class receptor 8a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015526">
        <property type="entry name" value="Frizzled/SFRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000539">
        <property type="entry name" value="Frizzled/Smoothened_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309">
        <property type="entry name" value="FRIZZLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309:SF136">
        <property type="entry name" value="FRIZZLED-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01534">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00489">
        <property type="entry name" value="FRIZZLED"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01330">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="13 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 20" id="KW-0732">Signal</keyword>
    <keyword evidence="15" id="KW-0807">Transducer</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012203707" description="Frizzled-5" evidence="20">
        <location>
            <begin position="28"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="318"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="451"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="500"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="FZ" evidence="21">
        <location>
            <begin position="29"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="22">
        <location>
            <begin position="234"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="153"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="173"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="34"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="42"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="79"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="106"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="110"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_017325896.1"/>
        </source>
    </evidence>
    <sequence length="581" mass="64770" checksum="3A6B554E490BAF38" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R9I5</accession>
    <name>A0A2D0R9I5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DEP domain-containing mTOR-interacting protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">deptor</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
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        <property type="nucleotide sequence ID" value="XM_017471001.3"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:GTPase activator activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023051">
        <property type="term" value="P:regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="PDZ_DEPTOR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DEP domain-containing mTOR-interacting protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
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        <property type="entry name" value="DEP_dom"/>
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        <property type="entry name" value="mTOR-Rac_regulators"/>
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        <property type="entry name" value="PDZ"/>
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        <property type="entry name" value="PDZ_sf"/>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22829">
        <property type="entry name" value="DEP DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22829:SF18">
        <property type="entry name" value="DEP DOMAIN-CONTAINING MTOR-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
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    </dbReference>
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        <property type="entry name" value="DEP"/>
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    </dbReference>
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        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEP" evidence="3">
        <location>
            <begin position="32"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="DEP" evidence="3">
        <location>
            <begin position="139"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="318"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017326490.2"/>
        </source>
    </evidence>
    <sequence length="396" mass="45134" checksum="8464DA163D85EBDD" modified="2023-06-28" version="2">MDSIGNSMQNKAAELERMAEVLFTGEQLRLRLHEGKVIKDRRHHLRTYPNCFVAKELIDWLIEHKEASDRDMAIKIMQKLLDQNIIHHVCDEHTEFKDMKLFYRFRKDDGTFPLDSEAKVFMRGQRIYEKLMNAESNLLKTREEESVPHERTLVASEFIDWLLQEGEMATRAEAEQLGRRLLEHGIIQHVTNKHHFADGDLLFQFRLNFRRRRRLMELLNERTRVIPESHDSPFCLRKQNMDSSNNSFLSVSPTKEIKVVSAVRRSSMSSSCGSSGYYSSSPTLSSSPPVLCNPKSVLKRQVSPEELQTPGGPFIKKTFTIIGDAVGWGFVVRGNKPCHIQAVDPSGPAAAAGMKVCQFVVSVNGLNVLNLDYKTVSNLILTGPKTVVMEVMEECQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RDS0</accession>
    <name>A0A2D0RDS0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Forkhead box C1-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">foxc1a</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017328689.1">
        <property type="nucleotide sequence ID" value="XM_017473200.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RDS0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018397"/>
    <dbReference type="GeneID" id="108268309"/>
    <dbReference type="KEGG" id="ipu:108268309"/>
    <dbReference type="CTD" id="100148408"/>
    <dbReference type="OMA" id="TSWYLNQ"/>
    <dbReference type="OrthoDB" id="5954824at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20044">
        <property type="entry name" value="FH_FOXC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000016">
        <property type="entry name" value="Forkhead box protein I1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047391">
        <property type="entry name" value="FOXC1/C2-like_FH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF68">
        <property type="entry name" value="FORKHEAD BOX PROTEIN C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="8">
        <location>
            <begin position="75"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="75"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="175"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="426"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="298"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="342"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="358"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="426"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017328689.1"/>
        </source>
    </evidence>
    <sequence length="523" mass="57222" checksum="9DFBBCF2DCD78BF1" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RE16</accession>
    <name>A0A2D0RE16_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein transport protein Sec61 subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC108268140</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of SEC61 channel-forming translocon complex that mediates transport of signal peptide-containing precursor polypeptides across the endoplasmic reticulum (ER). Forms a ribosome receptor and a gated pore in the ER membrane, both functions required for cotranslational translocation of nascent polypeptides. The SEC61 channel is also involved in ER membrane insertion of transmembrane proteins: it mediates membrane insertion of the first few transmembrane segments of proteins, while insertion of subsequent transmembrane regions of multi-pass membrane proteins is mediated by the multi-pass translocon (MPT) complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">The SEC61 channel-forming translocon complex consists of channel-forming core components SEC61A1, SEC61B and SEC61G and different auxiliary components such as SEC62 and SEC63. The SEC61 channel associates with the multi-pass translocon (MPT) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017328381.1">
        <property type="nucleotide sequence ID" value="XM_017472892.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RE16"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006317"/>
    <dbReference type="GeneID" id="108268140"/>
    <dbReference type="KEGG" id="ipu:108268140"/>
    <dbReference type="OrthoDB" id="2401875at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.820:FF:000001">
        <property type="entry name" value="Transport protein Sec61 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.820">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023391">
        <property type="entry name" value="Prot_translocase_SecE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008158">
        <property type="entry name" value="Translocase_Sec61-g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00327">
        <property type="entry name" value="secE_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12309">
        <property type="entry name" value="SEC61 GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103456">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01067">
        <property type="entry name" value="SECE_SEC61G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017328381.1"/>
        </source>
    </evidence>
    <sequence length="68" mass="7725" checksum="065EA388B4D08561" modified="2017-12-20" version="1">MDQIMQFVEPGRQFVKDSIRLVKRCTKPDRKEFQKIAMATAIGFAIMGFIGFFVKLIHIPINNIIVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RJW5</accession>
    <name>A0A2D0RJW5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit KCP2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Keratinocyte-associated protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">krtcap2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Subunit of STT3A-containing oligosaccharyl transferase (OST-A) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Within the OST-A complex, acts as an adapter that anchors the OST-A complex to the Sec61 complex. May be involved in N-glycosylation of APP (amyloid-beta precursor protein). Can modulate gamma-secretase cleavage of APP by enhancing endoprotelysis of PSEN1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of STT3A-containing oligosaccharyl transferase (OST-A) complex. STT3A-containing complex assembly occurs through the formation of 3 subcomplexes. Subcomplex 1 contains RPN1 and TMEM258, subcomplex 2 contains the STT3A-specific subunits STT3A, DC2/OSTC, and KCP2 as well as the core subunit OST4, and subcomplex 3 contains RPN2, DAD1, and OST48. The OST-A complex can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes. Interacts with PSEN1 and NCSTN; indicative for an association with the gamma-secretase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KRTCAP2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017330215.1">
        <property type="nucleotide sequence ID" value="XM_017474726.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RJW5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005037"/>
    <dbReference type="GeneID" id="108269131"/>
    <dbReference type="KEGG" id="ipu:108269131"/>
    <dbReference type="CTD" id="200185"/>
    <dbReference type="OMA" id="ITIYYMN"/>
    <dbReference type="OrthoDB" id="1111004at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018614">
        <property type="entry name" value="KRTCAP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32001">
        <property type="entry name" value="KERATINOCYTE-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32001:SF1">
        <property type="entry name" value="KERATINOCYTE-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09775">
        <property type="entry name" value="Keratin_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="75"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00063768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017330215.1"/>
        </source>
    </evidence>
    <sequence length="136" mass="14701" checksum="9454C4A252041BB8" modified="2017-12-20" version="1">MAVNTGTSLVLSSLLSVLVFAGMQMFSRQLASSEWLTILGGFLGSLLFICSLTAFNNLENLVFGKGFQAKIFPEIFLCLCLSLFASGLVHRVCVTTCLIFSLFALYYINKVSAALYQMAAPVATPAKITGKGKRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RWV6</accession>
    <name>A0A2D0RWV6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="17">ATP-PFK</shortName>
            <shortName evidence="17">Phosphofructokinase</shortName>
            <ecNumber evidence="17">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">pfklb</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17 18">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="17">Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 17 18">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homo- and heterotetramers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="17"/>
    <dbReference type="RefSeq" id="XP_017334435.1">
        <property type="nucleotide sequence ID" value="XM_017478946.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RWV6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032344"/>
    <dbReference type="GeneID" id="108271376"/>
    <dbReference type="KEGG" id="ipu:108271376"/>
    <dbReference type="CTD" id="550259"/>
    <dbReference type="OMA" id="CERLGEX"/>
    <dbReference type="OrthoDB" id="537915at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005945">
        <property type="term" value="C:6-phosphofructokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070095">
        <property type="term" value="F:fructose-6-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00764">
        <property type="entry name" value="Eukaryotic_PFK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000001">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000003">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.450:FF:000043">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase, platelet type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="entry name" value="Phosphofructokinase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03184">
        <property type="entry name" value="Phosphofructokinase_I_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009161">
        <property type="entry name" value="6-Pfructokinase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041914">
        <property type="entry name" value="PFK_vert-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015912">
        <property type="entry name" value="Phosphofructokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02478">
        <property type="entry name" value="6PF1K_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697:SF14">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE, LIVER TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000533">
        <property type="entry name" value="ATP_PFK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00433">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 17" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="10 17 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 17" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 17 18" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 17 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 17 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase" evidence="19">
        <location>
            <begin position="17"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="19">
        <location>
            <begin position="401"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal catalytic PFK domain 1" evidence="17">
        <location>
            <begin position="1"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal regulatory PFK domain 2" evidence="17">
        <location>
            <begin position="400"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="87"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="117"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="163"/>
            <end position="165"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="207"/>
            <end position="209"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="297"/>
            <end position="300"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="526"/>
            <end position="530"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="564"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="571"/>
            <end position="573"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <position position="627"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="653"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <begin position="659"/>
            <end position="662"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="17">
        <location>
            <position position="733"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR000533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017334435.1"/>
        </source>
    </evidence>
    <sequence length="779" mass="85158" checksum="D2A88E0A0B372B79" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RXN6</accession>
    <name>A0A2D0RXN6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serotransferrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">sxph</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Transferrins are iron binding transport proteins which bind Fe(3+) ion in association with the binding of an anion, usually bicarbonate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the transferrin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017335262.1">
        <property type="nucleotide sequence ID" value="XM_017479773.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RXN6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000025886"/>
    <dbReference type="GeneID" id="108271876"/>
    <dbReference type="KEGG" id="ipu:108271876"/>
    <dbReference type="CTD" id="100000381"/>
    <dbReference type="OMA" id="ESCNLGV"/>
    <dbReference type="OrthoDB" id="5914301at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13529">
        <property type="entry name" value="PBP2_transferrin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016357">
        <property type="entry name" value="Transferrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001156">
        <property type="entry name" value="Transferrin-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11485:SF28">
        <property type="entry name" value="OVOTRANSFERRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11485">
        <property type="entry name" value="TRANSFERRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00405">
        <property type="entry name" value="Transferrin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002549">
        <property type="entry name" value="Transferrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00422">
        <property type="entry name" value="TRANSFERRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00094">
        <property type="entry name" value="TR_FER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51408">
        <property type="entry name" value="TRANSFERRIN_LIKE_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0410">Iron transport</keyword>
    <keyword evidence="4 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012429248" description="Serotransferrin" evidence="8">
        <location>
            <begin position="21"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin-like" evidence="9">
        <location>
            <begin position="23"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin-like" evidence="9">
        <location>
            <begin position="367"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="490"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="491"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="26"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="133"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="176"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="189"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="254"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="370"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="380"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="428"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="482"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="510"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="524"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="533"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="611"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR002549-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR002549-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR002549-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017335262.1"/>
        </source>
    </evidence>
    <sequence length="726" mass="79803" checksum="26DDCF54888E29B3" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RZQ0</accession>
    <name>A0A2D0RZQ0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Leucine-rich repeat-containing protein 40</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">lrrc40</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017335967.1">
        <property type="nucleotide sequence ID" value="XM_017480478.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RZQ0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012324"/>
    <dbReference type="GeneID" id="108272205"/>
    <dbReference type="KEGG" id="ipu:108272205"/>
    <dbReference type="CTD" id="55631"/>
    <dbReference type="OMA" id="CMLHKLT"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000116">
        <property type="entry name" value="Leucine-rich repeat-containing protein 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000193">
        <property type="entry name" value="Leucine-rich repeat-containing protein 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000206">
        <property type="entry name" value="leucine-rich repeat-containing protein 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000265">
        <property type="entry name" value="Leucine-rich repeat-containing protein 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00071450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017335967.1"/>
        </source>
    </evidence>
    <sequence length="601" mass="67127" checksum="321177A682C7796C" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S6A0</accession>
    <name>A0A2D0S6A0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transmembrane protein 100</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC108273509</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017338273.1">
        <property type="nucleotide sequence ID" value="XM_017482784.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S6A0"/>
    <dbReference type="GeneID" id="108273509"/>
    <dbReference type="KEGG" id="ipu:108273509"/>
    <dbReference type="OrthoDB" id="8843254at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071773">
        <property type="term" value="P:cellular response to BMP stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032536">
        <property type="entry name" value="TMEM100"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16100">
        <property type="entry name" value="PHOSPHOINOSITIDE-INTERACTING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16100:SF5">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16311">
        <property type="entry name" value="TMEM100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017338273.1"/>
        </source>
    </evidence>
    <sequence length="137" mass="14805" checksum="9A5B5B29FE8BEFCD" modified="2017-12-20" version="1">MGCSSGRLPCQPPASAQLPTLDPDSNLLHDGRAPPTTPKTPENLSTLDRLTQATGGTEKSWYRCVFPFGVISLVIGMAGTGVTFTFNTLLQTKVVSLVLLVVGFVMLLVAAACWRVHRAKRKEKKEGVFFSAEQNTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S6B6</accession>
    <name>A0A2D0S6B6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">LOW QUALITY PROTEIN: serine/threonine-protein kinase H1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">si:ch211-27e6.1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017338299.2">
        <property type="nucleotide sequence ID" value="XM_017482810.2"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000015603"/>
    <dbReference type="GeneID" id="108273536"/>
    <dbReference type="KEGG" id="ipu:108273536"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="14"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017338299.2"/>
        </source>
    </evidence>
    <sequence length="301" mass="34298" checksum="84846E7358337368" modified="2023-06-28" version="2">MFRTRFDPRITSRYDIKALIGRGSFSHVVRVEHRYTHQPFAIKLLEVREHKGHDTCHVELGILQRVHHANIIHLVEVFETPHHVYLVLELATGGVLLDCVVSXGSFTEHDATKALIMVSRGLSYIHALGVIHQDLKPENLLHYHPGQDSRLIITDFGLACWSQRSQHWALRTLCGTPEYMAPEMVAGSTYGCEVDMWAMGMISYIVLSGSMPFEQRSRPRLFTAILRGSYTFHGQPWSSISDQAKDFIDHVLSVNPDQRMSAEQALKHHWLRNMAACSSNMNLHWPISQNFPSAGLMRLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S8W7</accession>
    <name>A0A2D0S8W7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mix-type homeobox gene 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">mxtx2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017339189.1">
        <property type="nucleotide sequence ID" value="XM_017483700.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S8W7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026495"/>
    <dbReference type="GeneID" id="108273977"/>
    <dbReference type="KEGG" id="ipu:108273977"/>
    <dbReference type="CTD" id="58078"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042074">
        <property type="term" value="P:cell migration involved in gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060027">
        <property type="term" value="P:convergent extension involved in gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001706">
        <property type="term" value="P:endoderm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055113">
        <property type="term" value="P:epiboly involved in gastrulation with mouth forming second"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000312">
        <property type="entry name" value="Mix-type homeobox gene 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051306">
        <property type="entry name" value="Homeobox_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46123">
        <property type="entry name" value="MIX-TYPE HOMEOBOX GENE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46123:SF4">
        <property type="entry name" value="MIX-TYPE HOMEOBOX GENE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="18"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="20"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="161"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="162"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017339189.1"/>
        </source>
    </evidence>
    <sequence length="305" mass="33623" checksum="714A6B73E799D06D" modified="2017-12-20" version="1">MWSDSIVGKDGAAQASKIAGRRKRTSFTKEHLELLRMAFNVDPYPGISVRESLSQATGLPESRIQVWFQNKRARTLKNRNILTSPQPEAASSLPSPFLPPPMVGIRENRQPAFNITQPQLREDCEQDCFFTDLSPQSFGLPSSGAHCSTPSIRPRQDRLMGTSLSPSSFQSDSEVTPAGWGSMRAQTSPECLWSPPMQGTGNNSKDESQVFLYPPPPYPHGSVRSGFLNSLKSTSPDSADSAIWDMGVENTSPMPYFQGGSGFWDESAQEQPLAPLPNLSSQCLEEVLGEMKPGWWKINGQMELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SB08</accession>
    <name>A0A2D0SB08_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Synaptopodin 2-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">synpo2lb</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Actin-associated protein that may play a role in modulating actin-based shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the synaptopodin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017339923.1">
        <property type="nucleotide sequence ID" value="XM_017484434.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SB08"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006009"/>
    <dbReference type="GeneID" id="108274328"/>
    <dbReference type="KEGG" id="ipu:108274328"/>
    <dbReference type="CTD" id="100001241"/>
    <dbReference type="OMA" id="PGPYQGV"/>
    <dbReference type="OrthoDB" id="445995at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032233">
        <property type="term" value="P:positive regulation of actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10820">
        <property type="entry name" value="PDZ_SYNPO2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000137">
        <property type="entry name" value="Synaptopodin 2-like a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051976">
        <property type="entry name" value="Synaptopodin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24217">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24217:SF10">
        <property type="entry name" value="SYNAPTOPODIN 2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="11">
        <location>
            <begin position="5"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="115"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="252"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="462"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="914"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1089"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="194"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="463"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="477"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="914"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1172"/>
            <end position="1193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017339923.1"/>
        </source>
    </evidence>
    <sequence length="1205" mass="129118" checksum="925B10D6AF199034" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SBW6</accession>
    <name>A0A2D0SBW6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PDZ domain-containing RING finger protein 4 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">pdzrn4</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017340222.1">
        <property type="nucleotide sequence ID" value="XM_017484733.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SBW6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021412"/>
    <dbReference type="GeneID" id="108274525"/>
    <dbReference type="KEGG" id="ipu:108274525"/>
    <dbReference type="CTD" id="29951"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="CDD" id="cd06716">
        <property type="entry name" value="PDZ2-PDZRN4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000028">
        <property type="entry name" value="PDZ domain containing ring finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051971">
        <property type="entry name" value="E3_ubiquitin-PDZ_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15545">
        <property type="entry name" value="PDZ DOMAIN CONTAINING RING FINGER PROTEIN 3, 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15545:SF6">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING RING FINGER PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="143"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="255"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="412"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="498"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="382"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="413"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="438"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="451"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="511"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017340222.1"/>
        </source>
    </evidence>
    <sequence length="741" mass="84786" checksum="8AEB791BD3435E77" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SBY9</accession>
    <name>A0A2D0SBY9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Delta-1-pyrroline-5-carboxylate synthase</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="20">Glutamate 5-kinase</fullName>
                <shortName evidence="20">GK</shortName>
                <ecNumber evidence="20">2.7.2.11</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="20">Gamma-glutamyl kinase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="20">Gamma-glutamyl phosphate reductase</fullName>
                <shortName evidence="20">GPR</shortName>
                <ecNumber evidence="20">1.2.1.41</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="20">Glutamate-5-semialdehyde dehydrogenase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="20">Glutamyl-gamma-semialdehyde dehydrogenase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="24" type="primary">aldh18a1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Bifunctional enzyme that converts glutamate to glutamate 5-semialdehyde, an intermediate in the biosynthesis of proline, ornithine and arginine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 20">
            <text>L-glutamate + ATP = L-glutamyl 5-phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14877"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 20">
            <text>L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19541"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 20">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 20">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Can form homodimers/multimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 20">In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 20">In the N-terminal section; belongs to the glutamate 5-kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.11" evidence="20"/>
    <dbReference type="EC" id="1.2.1.41" evidence="20"/>
    <dbReference type="RefSeq" id="XP_017339745.2">
        <property type="nucleotide sequence ID" value="XM_017484256.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SBY9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000023951"/>
    <dbReference type="GeneID" id="108274232"/>
    <dbReference type="KEGG" id="ipu:108274232"/>
    <dbReference type="CTD" id="5832"/>
    <dbReference type="OrthoDB" id="1934954at2759"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00359"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004349">
        <property type="term" value="F:glutamate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004350">
        <property type="term" value="F:glutamate-5-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04256">
        <property type="entry name" value="AAK_P5CS_ProBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07079">
        <property type="entry name" value="ALDH_F18-19_ProA-GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000010">
        <property type="entry name" value="Delta-1-pyrroline-5-carboxylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000011">
        <property type="entry name" value="Delta-1-pyrroline-5-carboxylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00412">
        <property type="entry name" value="ProA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00456">
        <property type="entry name" value="ProB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020593">
        <property type="entry name" value="G-glutamylP_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041744">
        <property type="entry name" value="G5K_ProBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001057">
        <property type="entry name" value="Glu/AcGlu_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005715">
        <property type="entry name" value="Glu_5kinase/COase_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019797">
        <property type="entry name" value="Glutamate_5-kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000965">
        <property type="entry name" value="GPR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005766">
        <property type="entry name" value="P5_carboxy_syn"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01092">
        <property type="entry name" value="P5CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001221">
        <property type="entry name" value="PRK00197.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00407">
        <property type="entry name" value="proA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01027">
        <property type="entry name" value="proB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063:SF8">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063">
        <property type="entry name" value="GLUTAMATE SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036429">
        <property type="entry name" value="P5C_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00474">
        <property type="entry name" value="GLU5KINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00902">
        <property type="entry name" value="GLUTAMATE_5_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01223">
        <property type="entry name" value="PROA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 20" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12 20" id="KW-0521">NADP</keyword>
    <keyword evidence="9 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 20" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 20" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/glutamate/uridylate kinase" evidence="22">
        <location>
            <begin position="74"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="21">
        <location>
            <begin position="365"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00055158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00064272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRNR" id="PIRNR036429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_017339745.2"/>
        </source>
    </evidence>
    <sequence length="805" mass="87722" checksum="72BB663D0B3D9B68" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SDR5</accession>
    <name>A0A2D0SDR5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">L-fucose kinase</fullName>
            <ecNumber evidence="8">2.7.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC108274775</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Takes part in the salvage pathway for reutilization of fucose from the degradation of oligosaccharides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-fucose + ATP = beta-L-fucose 1-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13241"/>
            <dbReference type="ChEBI" id="CHEBI:2181"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57268"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.52"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the GHMP kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.52" evidence="8"/>
    <dbReference type="RefSeq" id="XP_017340606.2">
        <property type="nucleotide sequence ID" value="XM_017485117.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SDR5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022269"/>
    <dbReference type="GeneID" id="108274775"/>
    <dbReference type="KEGG" id="ipu:108274775"/>
    <dbReference type="OrthoDB" id="271303at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050201">
        <property type="term" value="F:fucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042352">
        <property type="term" value="P:GDP-L-fucose salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.120:FF:000001">
        <property type="entry name" value="L-fucose kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012887">
        <property type="entry name" value="GDP_fucose_pyrophosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052203">
        <property type="entry name" value="GHMP_Kinase-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32463">
        <property type="entry name" value="L-FUCOSE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32463:SF0">
        <property type="entry name" value="L-FUCOSE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07959">
        <property type="entry name" value="Fucose_pyrophosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GDP-fucose pyrophosphorylase" evidence="11">
        <location>
            <begin position="100"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="10">
        <location>
            <begin position="837"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="12">
        <location>
            <begin position="988"/>
            <end position="1064"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00052616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00059365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017340606.2"/>
        </source>
    </evidence>
    <sequence length="1098" mass="118415" checksum="BAF7CA4E292B7296" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SJ62</accession>
    <name>A0A2D0SJ62_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RNA uridylyltransferase</fullName>
            <ecNumber evidence="5">2.7.7.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21 22" type="primary">tut4</name>
    </gene>
    <organism evidence="20 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21 22" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21 22">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>RNA(n) + UTP = RNA(n)-3'-uridine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14785"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17348"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173116"/>
            <dbReference type="EC" id="2.7.7.52"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DNA polymerase type-B-like family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.52" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017342731.1">
        <property type="nucleotide sequence ID" value="XM_017487242.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017342732.1">
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        <property type="term" value="F:RNA uridylyltransferase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:RNA 3'-end processing"/>
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        <property type="entry name" value="Beta Polymerase, domain 2"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="3"/>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 21 22" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="12 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="19">
        <location>
            <begin position="762"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="19">
        <location>
            <begin position="1142"/>
            <end position="1157"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="19">
        <location>
            <begin position="1185"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="493"/>
            <end position="531"/>
        </location>
    </feature>
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        <location>
            <begin position="622"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1157"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1225"/>
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        <location>
            <begin position="1329"/>
            <end position="1363"/>
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        <location>
            <begin position="33"/>
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    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="51"/>
            <end position="60"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="89"/>
            <end position="114"/>
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            <begin position="151"/>
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            <begin position="493"/>
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        <location>
            <begin position="1267"/>
            <end position="1297"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017342731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017342732.1"/>
        </source>
    </evidence>
    <sequence length="1483" mass="167185" checksum="E56EA351AB3269FC" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SKX0</accession>
    <name>A0A2D0SKX0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Homeobox protein Hox-C11a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">hoxc11a</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Abd-B homeobox family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017487642.3"/>
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        <property type="component" value="Chromosome 15"/>
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        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000166">
        <property type="entry name" value="homeobox protein Hox-C11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021918">
        <property type="entry name" value="DUF3528"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46092">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-A11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46092:SF1">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-C11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12045">
        <property type="entry name" value="DUF3528"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="233"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="235"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="177"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="124"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="182"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="196"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="219"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017343131.2"/>
        </source>
    </evidence>
    <sequence length="307" mass="35323" checksum="D6AEE45962C38056" modified="2023-06-28" version="2">MFNSVNLSNFCSQTRKDRTSEFGDRTGCASNIYLPSCTYYVPEFSTVSSFLPQGPSRQITYPYTTNLSQVQPVRDVSYGLDPSSKWHHRTNYATCYSGEDLVHRDCLPPSTMTEMLMKNESVYSHHHHHHHPSSNHPSTGFYSSVGKNNVLPQGFDRFFESAYCGADNQSDHCLQKSETTKLESTSQQPSAVSGVEEPEKDAEDEEERTNSGSCSSSGTKESNASKINHSSAPRTRKKRCPYSKFQIRELEREFFFNVYINKEKRLQLSRMLNLTDRQVKIWFQNRRMKEKKLSRDRLQYFSGNPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SMC0</accession>
    <name>A0A2D0SMC0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Osteoclast-stimulating factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">grap2a</name>
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    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Induces bone resorption, acting probably through a signaling cascade which results in the secretion of factor(s) enhancing osteoclast formation and activity.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017488152.3"/>
    </dbReference>
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    <dbReference type="STRING" id="7998.ENSIPUP00000019871"/>
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    <dbReference type="OrthoDB" id="10255964at2759"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="entry name" value="SH2_Grb2_like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Unconventional Myosin IB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="SH2"/>
    </dbReference>
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        <property type="entry name" value="SH2_dom_sf"/>
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        <property type="entry name" value="SH3_domain"/>
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        <property type="entry name" value="PROTEIN ENHANCER OF SEVENLESS 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1 8" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
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        <location>
            <begin position="58"/>
            <end position="149"/>
        </location>
    </feature>
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        <location>
            <begin position="221"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="143"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="143"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037432"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00040640"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017343641.1"/>
        </source>
    </evidence>
    <sequence length="278" mass="31574" checksum="8F74AC40DA50A30F" modified="2017-12-20" version="1">MEARGKFDFSATAEDELSFRKGDILKILGAQDDWYKAEMHGHEGFVPKNYVDRQTPSWFQENASRGAAEEILMSREVGGFLIRGSQSSPGDFSISVRHEFDVQHFKVMKDNKGNYYLWSEKFMSLNKLVEFYKTTSISKQREIFLRDGSREDPAPAPHPVKRGSDRPQPSAHRRPPEQAHTQQKQGSLEERTHNMGPTGKNSPVNPLPTSRRPSDNMPAPRLSVQVKALYDFIAEEDDELGFSAGDVIEVLDRSEASWWKGRLRGNVGLFPSNYTVQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SQA5</accession>
    <name>A0A2D0SQA5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Interleukin-11 receptor subunit alpha isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">il11ra</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type I cytokine receptor family. Type 3 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017344681.1">
        <property type="nucleotide sequence ID" value="XM_017489192.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SQA5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000037179"/>
    <dbReference type="GeneID" id="108276979"/>
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    <dbReference type="CTD" id="3590"/>
    <dbReference type="OrthoDB" id="418412at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061026">
        <property type="term" value="P:cardiac muscle tissue regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0031101">
        <property type="term" value="P:fin regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="FN3_dom"/>
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        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_sub"/>
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    <dbReference type="InterPro" id="IPR053073">
        <property type="entry name" value="IL11/IL27_subunit_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48483">
        <property type="entry name" value="INTERLEUKIN-27 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48483:SF2">
        <property type="entry name" value="INTERLEUKIN-27 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017344681.1"/>
        </source>
    </evidence>
    <sequence length="411" mass="45580" checksum="9E52E8C84C9DC12F" modified="2017-12-20" version="1">MPGLVSCPGGLIVIGILALSCVHANSEILSNEVSDVQFGTLGSTVTLTCRGSHQGSAVVWTHNGSLVRTRQTHPGWDGSLTLTNTTRSMEGNYSCHKPRGGALLQSITLRLGYAPEYLSVSCKLPSHFKMLCTWTQRVRTHLPTKYISSYSVDNSRPEPCEQELMDVNECTISNPVFWGSKHLVNITEVNPLGSKSTIAYVDVNKNLKPDAPEDLICKQVDTEPTQLVVQWKPPASWPSDVSNAFPLKFELQYRPVGSRFWSKVETEDTSMVIMDALMGHLHHIRVRAQDAFINHSQWSEWSQVVQAQPWSDLSTIPETTTDFFEPIFPSTFPVKATEKSLAALTAVPVVNQDPSFDENGRQVLIALGLLAAVMVVVVFTITVLLCVRNRRRGNMTKQELTSMVKMKSLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SQW0</accession>
    <name>A0A2D0SQW0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ribonuclease kappa-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rnasekb</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNase K family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017344886.1">
        <property type="nucleotide sequence ID" value="XM_017489397.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SQW0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007944"/>
    <dbReference type="GeneID" id="108277077"/>
    <dbReference type="KEGG" id="ipu:108277077"/>
    <dbReference type="CTD" id="336290"/>
    <dbReference type="OMA" id="ATQCWVA"/>
    <dbReference type="OrthoDB" id="67317at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056552">
        <property type="entry name" value="Ribonucl_Kappa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026770">
        <property type="entry name" value="RNase_K"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31733">
        <property type="entry name" value="RIBONUCLEASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23489">
        <property type="entry name" value="V-ATPase_su_f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017344886.1"/>
        </source>
    </evidence>
    <sequence length="101" mass="11018" checksum="5AD001D76B423D8C" modified="2017-12-20" version="1">MPSLLFCGPKLAACGIVLSIWGVVMLAMLGIFFSAKSAVLIEDVPFTEEDIRNDTNPPQAIYSLYNQVAINCFIAAAIYVGVGAISLCQVRLNKRQEYMVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SR17</accession>
    <name>A0A2D0SR17_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ciliary microtubule inner protein 2B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">fam166b</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CIMIP2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017345152.1">
        <property type="nucleotide sequence ID" value="XM_017489663.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SR17"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033453"/>
    <dbReference type="GeneID" id="108277175"/>
    <dbReference type="KEGG" id="ipu:108277175"/>
    <dbReference type="CTD" id="730112"/>
    <dbReference type="OMA" id="CCGQDLT"/>
    <dbReference type="OrthoDB" id="2019884at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018902">
        <property type="entry name" value="CMI2A-C-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22146">
        <property type="entry name" value="CAT EYE SYNDROME CRITICAL REGION PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22146:SF8">
        <property type="entry name" value="PROTEIN FAM166B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10629">
        <property type="entry name" value="CMI2B-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ciliary microtubule inner protein 2A-C-like" evidence="9">
        <location>
            <begin position="16"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Ciliary microtubule inner protein 2A-C-like" evidence="9">
        <location>
            <begin position="186"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Ciliary microtubule inner protein 2A-C-like" evidence="9">
        <location>
            <begin position="251"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="56"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="67"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017345152.1"/>
        </source>
    </evidence>
    <sequence length="291" mass="32599" checksum="B906640832684737" modified="2017-12-20" version="1">MEKFPPKFSRVLVTPDPHYIPGYAGYCPQLKFHMGMPYSPLTAQLLTSPEISHSPRMVLSSGLSPSTERDTGTREEVRTRGTGHGKTVQRMIPGYTGFIPRSQNYFSKSYTDTCREALSEFDSDQKRRIRPATADGLPAEFPDIKRRALNTPLVVISKEPLSYKSMDCWKPLGSPYSMEDDNPHKYFISGFTGYVPKARFLIGSSYPKTTNKALIQLGKQMKASHSAHGLTTESADNLVSLPSIYPTQQGLLPRYTGHVPGYKFRYGQTFGKLTSNSLELSGTRRKIDIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SVE4</accession>
    <name>A0A2D0SVE4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zona pellucida sperm-binding protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Zona pellucida glycoprotein 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Zona pellucida protein B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">LOC108278043</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP4 may act as a sperm receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Zona pellucida</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017346657.3">
        <property type="nucleotide sequence ID" value="XM_017491168.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SVE4"/>
    <dbReference type="SMR" id="A0A2D0SVE4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000016965"/>
    <dbReference type="GeneID" id="108278043"/>
    <dbReference type="KEGG" id="ipu:108278043"/>
    <dbReference type="OrthoDB" id="8919081at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035805">
        <property type="term" value="C:egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032190">
        <property type="term" value="F:acrosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035804">
        <property type="term" value="F:structural constituent of egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060468">
        <property type="term" value="P:prevention of polyspermy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00111">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.110.10">
        <property type="entry name" value="Spasmolytic Protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000519">
        <property type="entry name" value="P_trefoil_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044913">
        <property type="entry name" value="P_trefoil_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051148">
        <property type="entry name" value="Zona_Pellucida_Domain_gp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055356">
        <property type="entry name" value="ZP-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23343">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23343:SF31">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00088">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23344">
        <property type="entry name" value="ZP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00018">
        <property type="entry name" value="PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57492">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51448">
        <property type="entry name" value="P_TREFOIL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="9 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="11" id="KW-0278">Fertilization</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039953007" description="Zona pellucida sperm-binding protein 4" evidence="19">
        <location>
            <begin position="23"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="P-type" evidence="21">
        <location>
            <begin position="54"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="20">
        <location>
            <begin position="97"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="66"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017346657.3"/>
        </source>
    </evidence>
    <sequence length="412" mass="44482" checksum="FE4DBC27A45830C2" modified="2023-06-28" version="3">MANTRVSLVLVGIFLVFNYVSSTPASPQAVKMPPQAPQLSGKPTLQPQIPGQVVKCQVPDYERVPCGEPGINSDQCTTINCCFDGQQCYYGKMVTVQCTLDGQFVLVVARDATVPRISLESISMLGGNTGPCSPVDSNTAFAIYQFPVTACGTTMMVQGGYVFYENRMLSSYEVGVGPRGAITRDTHYELYFQCKYSGVSLATLAIDPSANADPPSIVASGPLQVELRLGNGHCVAKGCAEDEVAYTSYYSAADYPVTKVLREPVYVEVHIVGRTDPNIVLVLGGCWATASPNPYSLPQWDLLVNGCPYKDDRYLTKLMPVNRMSGLAYPTHYRRFVLKMFTFVAPDSMAPMHETLYIHCSTAVCLPTAQGSCEPTCARRRRDAAAEGSAHTSGVVSSGGVIFTQSSSPQFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SX37</accession>
    <name>A0A2D0SX37_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">4-aminobutyrate aminotransferase, mitochondrial</fullName>
            <ecNumber evidence="5">2.6.1.19</ecNumber>
            <ecNumber evidence="4">2.6.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">(S)-3-amino-2-methylpropionate transaminase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">GABA aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Gamma-amino-N-butyrate transaminase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">L-AIBAT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">abat</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(S)-3-amino-2-methylpropanoate + 2-oxoglutarate = 2-methyl-3-oxopropanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13993"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57700"/>
            <dbReference type="ChEBI" id="CHEBI:58655"/>
            <dbReference type="EC" id="2.6.1.22"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:13994"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23352"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
            <dbReference type="EC" id="2.6.1.19"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:23353"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.19" evidence="5"/>
    <dbReference type="EC" id="2.6.1.22" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017347249.1">
        <property type="nucleotide sequence ID" value="XM_017491760.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SX37"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022243"/>
    <dbReference type="GeneID" id="108278404"/>
    <dbReference type="KEGG" id="ipu:108278404"/>
    <dbReference type="CTD" id="18"/>
    <dbReference type="OrthoDB" id="5419315at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047298">
        <property type="term" value="F:(S)-3-amino-2-methylpropionate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034386">
        <property type="term" value="F:4-aminobutyrate:2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000029">
        <property type="entry name" value="4-aminobutyrate aminotransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004631">
        <property type="entry name" value="4NH2But_aminotransferase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00699">
        <property type="entry name" value="GABAtrns_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43206:SF1">
        <property type="entry name" value="4-AMINOBUTYRATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43206">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 20" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="9" id="KW-0531">Neurotransmitter degradation</keyword>
    <keyword evidence="10 18" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017347249.1"/>
        </source>
    </evidence>
    <sequence length="498" mass="55731" checksum="D8EB85069521EF8E" modified="2017-12-20" version="1">MALLSHHLTLSLRRNLRLVASGCRYVSQTATKTVSEIEYDAPSMKTPVPGPRSKELTRQLGEIQNVGAINFFCNYEESRGNYLVDADGNRMLDLYTQISSVPLGYNHPALMKALANPNNLSAFVNRPALGILPPENFPEKLTESLLSIAPIGMTRVQTMVCGSCSNENAFKAMFIWYKNKERGTNNPSAEETSSCMINQSPGCPDLSILSFMGAFHGRTFGCLATTHSKVIHKLDIPSLDWPIAPFPRLKYPLEEFVQENAREEARCLEEVEDLIVKWRQKGKPVAGIVIEPIQAEGGDNHASPDFFIKLRSIARKHGCAFHVDEVQTGGGATGKFWAHEHWGTDDPADIISFSKKMLTGGYYHTDEMQPDKGYRIFNTWMGDPSKNLFLSEVLNVIRRENLLEEVTRCGKALLQGLYELQSQYPHLLSRARGQGTFCAVDACNDVTRDNLLVKIRDKGVLLGGCGEKSIRVRPALIFKEYHAHQFLNIFNDVLSEYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T533</accession>
    <name>A0A2D0T533_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Adenosylhomocysteinase like 2b isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ahcyl2b</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017350017.1">
        <property type="nucleotide sequence ID" value="XM_017494528.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T533"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019906"/>
    <dbReference type="GeneID" id="108279907"/>
    <dbReference type="KEGG" id="ipu:108279907"/>
    <dbReference type="CTD" id="394240"/>
    <dbReference type="OMA" id="IVITCTX"/>
    <dbReference type="OrthoDB" id="10007170at2759"/>
    <dbReference type="UniPathway" id="UPA00314">
        <property type="reaction ID" value="UER00076"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004013">
        <property type="term" value="F:adenosylhomocysteinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033353">
        <property type="term" value="P:S-adenosylmethionine cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00401">
        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1480:FF:000002">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1480:FF:000007">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000035">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1480:FF:000009">
        <property type="entry name" value="Adenosylhomocysteinase like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00936">
        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004005">
        <property type="entry name" value="PRK05476.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420:SF2">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00738">
        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S-adenosyl-L-homocysteine hydrolase NAD binding" evidence="7">
        <location>
            <begin position="353"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="26"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017350017.1"/>
        </source>
    </evidence>
    <sequence length="594" mass="65719" checksum="5FAAC484BD1507F8" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T622</accession>
    <name>A0A2D0T622_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">nuak1a</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017350276.1">
        <property type="nucleotide sequence ID" value="XM_017494787.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T622"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009577"/>
    <dbReference type="GeneID" id="108280043"/>
    <dbReference type="KEGG" id="ipu:108280043"/>
    <dbReference type="CTD" id="561563"/>
    <dbReference type="OMA" id="EQDMAHI"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T789</accession>
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            <fullName evidence="2">Splicing factor Cactin</fullName>
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        <name type="scientific">Ictalurus punctatus</name>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="RefSeq"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the CACTIN family.</text>
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        <property type="entry name" value="Cactin_central"/>
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        <property type="entry name" value="POLYGLUTAMINE BINDING PROTEIN 1/MARVEL MEMBRANE-ASSOCIATING DOMAIN CONTAINING 3"/>
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    </dbReference>
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        <property type="entry name" value="SPLICING FACTOR CACTIN"/>
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    </dbReference>
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        <property type="entry name" value="Cactin_mid"/>
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        <property type="entry name" value="CactinC_cactus"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CactinC_cactus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    </feature>
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            <begin position="1"/>
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    </feature>
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        </location>
    </feature>
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            <begin position="311"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="27"/>
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        </location>
    </feature>
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        <location>
            <begin position="49"/>
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    </feature>
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        <location>
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        </location>
    </feature>
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    </feature>
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    </feature>
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    </feature>
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        <location>
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    </feature>
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    </feature>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
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            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006895"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034534"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF09732"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF10312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017350701.1"/>
        </source>
    </evidence>
    <sequence length="865" mass="101332" checksum="49AA1C48A55EB377" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T7Z6</accession>
    <name>A0A2D0T7Z6_ICTPU</name>
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        <submittedName>
            <fullName evidence="15 16">RNA-binding protein MEX3B</fullName>
        </submittedName>
    </protein>
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        <name evidence="15 16" type="primary">LOC108280492</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
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            <taxon>Siluriformes</taxon>
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        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
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    <reference evidence="15 16" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="15 16">Blood</tissue>
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        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Mex-3 RNA-binding family member C"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Mex-3 RNA-binding family member D"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="K Homology domain, type 1"/>
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        <property type="entry name" value="KH_dom"/>
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        <property type="entry name" value="zf-C3HC4_3"/>
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    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
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        <name evidence="13 14" type="primary">LOC108280892</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
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                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="12">SDA103</strain>
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                <consortium name="RefSeq"/>
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        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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    <dbReference type="PANTHER" id="PTHR24233:SF8">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24233">
        <property type="entry name" value="P2Y PURINOCEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCRRHODOPSN"/>
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    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
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    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
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    <keyword evidence="7 9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="127"/>
            <end position="151"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="233"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="274"/>
            <end position="293"/>
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        <location>
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            <end position="290"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000688"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017351877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017351878.1"/>
        </source>
    </evidence>
    <sequence length="337" mass="38166" checksum="88B5FE017041168A" modified="2017-12-20" version="1">MDLSNQQGDPLGVWSIVFVSLYVLVFVAGLLLNSMAVWVFFQIRTQKRTFMLYLRNMAVADLLMTLTLPINILTEAKVASSGLEGVSCRYTAVLFYCCMYASILFLGLVALDRYLKIVRPFGGTCGACLYSYSFTVAMAVMVWLVMIALSLPNVILTDKPVTKNMTNCAELKSEVGKKWHGVVIYINILIFFLVLITLLTCYISIYRHIHRSSSQFIIVGETTQRKAKKGQNIFVLLVVFFICFVPYHLCRIPFTKSQTDGVQMNFTLLNGKKATLLLSMFNVCLDPIIYFLMCKSFRSKLCQTLGFWTLSDKMSMYSPTSTRKTAVVMRFKESTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0TAW5</accession>
    <name>A0A2D0TAW5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nck-associated protein 5-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC108281005</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017352016.2">
        <property type="nucleotide sequence ID" value="XM_017496527.3"/>
    </dbReference>
    <dbReference type="GeneID" id="108281005"/>
    <dbReference type="KEGG" id="ipu:108281005"/>
    <dbReference type="OrthoDB" id="8930856at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001578">
        <property type="term" value="P:microtubule bundle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007019">
        <property type="term" value="P:microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032769">
        <property type="entry name" value="NCKAP5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026163">
        <property type="entry name" value="Nckap5l"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21740">
        <property type="entry name" value="NCK-ASSOCIATED PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21740:SF3">
        <property type="entry name" value="NCK-ASSOCIATED PROTEIN 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15246">
        <property type="entry name" value="NCKAP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nck-associated protein 5 C-terminal" evidence="2">
        <location>
            <begin position="923"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="473"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="729"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="832"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="973"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1175"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1251"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="351"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="431"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="498"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="573"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="583"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="605"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="633"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="749"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="836"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="982"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1261"/>
            <end position="1273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1283"/>
            <end position="1308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017352016.2"/>
        </source>
    </evidence>
    <sequence length="1308" mass="143048" checksum="B7261ACAE195801A" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979E764</accession>
    <name>A0A979E764_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Receptor-type tyrosine-protein phosphatase kappa</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">LOC108263831</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">SDA103</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="27">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Regulation of processes involving cell contact and adhesion such as growth control, tumor invasion, and metastasis. Negative regulator of EGFR signaling pathway. Forms complexes with beta-catenin and gamma-catenin/plakoglobin. Beta-catenin may be a substrate for the catalytic activity of PTPRK/PTP-kappa.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Receptor class 2B subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="RefSeq" id="XP_047010318.1">
        <property type="nucleotide sequence ID" value="XM_047154362.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108263831"/>
    <dbReference type="KEGG" id="ipu:108263831"/>
    <dbReference type="OrthoDB" id="10253954at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14635">
        <property type="entry name" value="R-PTPc-M-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000003">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase kappa isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000005">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase kappa isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000019">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase kappa isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000006">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase T isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000009">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase U isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000048">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase U isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057598">
        <property type="entry name" value="Fn3_PTPRU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051622">
        <property type="entry name" value="R-tyr_protein_phosphatases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24051:SF11">
        <property type="entry name" value="PROTEIN TYROSINE PHOSPHATASE, RECEPTOR TYPE, M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24051">
        <property type="entry name" value="SUSHI DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23144">
        <property type="entry name" value="Fn3_PTPRU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00020">
        <property type="entry name" value="MAMDOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 20" id="KW-0732">Signal</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036895811" description="Receptor-type tyrosine-protein phosphatase kappa" evidence="20">
        <location>
            <begin position="21"/>
            <end position="1458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="746"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1092"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="23">
        <location>
            <begin position="25"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="24">
        <location>
            <begin position="187"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="25">
        <location>
            <begin position="287"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="25">
        <location>
            <begin position="385"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="25">
        <location>
            <begin position="489"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="21">
        <location>
            <begin position="906"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="22">
        <location>
            <begin position="1080"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="21">
        <location>
            <begin position="1192"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="22">
        <location>
            <begin position="1368"/>
            <end position="1445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00059240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_047010318.1"/>
        </source>
    </evidence>
    <sequence length="1458" mass="165500" checksum="E6CD87CE9631897B" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EM82</accession>
    <name>A0A979EM82_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Trichohyalin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC124626273</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047006673.2">
        <property type="nucleotide sequence ID" value="XM_047150717.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EM82"/>
    <dbReference type="GeneID" id="124626273"/>
    <dbReference type="KEGG" id="ipu:124626273"/>
    <dbReference type="OrthoDB" id="691673at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47595">
        <property type="entry name" value="HEAT SHOCK 70 KDA PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47595:SF1">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="46"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="308"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="361"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_047006673.2"/>
        </source>
    </evidence>
    <sequence length="405" mass="47408" checksum="2B40721EA6C3B86E" modified="2023-06-28" version="2">MRIGSKSRLVGERHIIIWVGCLKHRSEIYSDLVGTATVKGWDMAGNWKDSETKELLSIRSEEEIIRQLNGTVRDAVVYEKITQKLKERGVLREKTQVINKLKTLRKKFHQIKQRNAQLGNSDWPYFDMCHYIWGGGRSANPVVLLSPLEPYPSENDAETTLSDTDILKTEVCTEFVPLSPAEFPPSPQSPPNKSAKKVSRGEQMVKDMKEFFTEMDRDFEERERLRVLEQRQYEECLRKEAKEEEREERARQMAMFKELLESQNNLLRELLLRIPSPTLQRLTPSKNNAEPETTSSVANIPDTKECTKFTHSHAESPQSPPNKRAKKVSQEEQIAKDMKECFAEIARTLEERERLRLLEHRQHEESLRKEAKQEAQEERARQMEMFKEMQESQNDLLKELLRRMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EZ37</accession>
    <name>A0A979EZ37_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MICOS complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 11 12 13" type="primary">LOC108269120</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11 12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11 12">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the apolipoprotein O/MICOS complex subunit Mic27 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047013000.2">
        <property type="nucleotide sequence ID" value="XM_047157044.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047013001.2">
        <property type="nucleotide sequence ID" value="XM_047157045.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053538132.1">
        <property type="nucleotide sequence ID" value="XM_053682157.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053538133.1">
        <property type="nucleotide sequence ID" value="XM_053682158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EZ37"/>
    <dbReference type="GeneID" id="108269120"/>
    <dbReference type="KEGG" id="ipu:108269120"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019166">
        <property type="entry name" value="MIC26/MIC27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033182">
        <property type="entry name" value="MIC26/MIC27_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14564">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC26 / MIC27 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09769">
        <property type="entry name" value="ApoO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044697461" description="MICOS complex subunit" evidence="8">
        <location>
            <begin position="26"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_047013000.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_047013001.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053538132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_053538133.1"/>
        </source>
    </evidence>
    <sequence length="256" mass="27770" checksum="58CEE4673ADB931D" modified="2023-06-28" version="2">MMAKVVKVVAVPVVLGFASLRITHSMSEDKPEVPLSPQQLSIYLTLPQERLRYSEEQPGLLQSSLSKFRWSLQSYVQKMKSACVSVKVTAVNLYYGGADVYHFLKDPPPGVVPRISVIAVSGLAGMILARKGSYLKRLGLPLSLASAGFALCYPVQTIAMLKVSGKKMHAASQWTTSTVASLRKQDTLPAVPVEEKLSSASSLTAESQPEMPCLQLSGVLQTPTAVPETQECEADLDVMDFGQSYPEDADLYSTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EZ54</accession>
    <name>A0A979EZ54_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dynamin-1-like protein</fullName>
            <ecNumber evidence="8">3.6.5.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="primary">dnm1l</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="29">SDA103</strain>
        </source>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="30">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Secretory vesicle</location>
            <location evidence="3">Synaptic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <location evidence="7">Clathrin-coated pit</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Mitochondrion outer membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="25">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.5" evidence="8"/>
    <dbReference type="RefSeq" id="XP_047013194.1">
        <property type="nucleotide sequence ID" value="XM_047157238.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EZ54"/>
    <dbReference type="GeneID" id="108269241"/>
    <dbReference type="CTD" id="10059"/>
    <dbReference type="OMA" id="CAYINTN"/>
    <dbReference type="OrthoDB" id="5061070at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043653">
        <property type="term" value="P:mitochondrial fragmentation involved in apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1240:FF:000001">
        <property type="entry name" value="Dynamin 1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000172">
        <property type="entry name" value="Dynamin-1-like protein isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003130">
        <property type="entry name" value="GED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF39">
        <property type="entry name" value="DYNAMIN-1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02212">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00302">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00410">
        <property type="entry name" value="G_DYNAMIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="22" id="KW-0168">Coated pit</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="23" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="15" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="19 25" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="11 25" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="28">
        <location>
            <begin position="22"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="27">
        <location>
            <begin position="634"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="538"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="586"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00011980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00018833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU003932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="RefSeq" id="XP_047013194.1"/>
        </source>
    </evidence>
    <sequence length="726" mass="81075" checksum="01A47AD7152706C2" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F359</accession>
    <name>A0A979F359_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Contactin-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">cntn3b</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="13">Interacts with PTPRG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. Contactin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053539700.1">
        <property type="nucleotide sequence ID" value="XM_053683725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979F359"/>
    <dbReference type="GeneID" id="108271503"/>
    <dbReference type="KEGG" id="ipu:108271503"/>
    <dbReference type="CTD" id="556251"/>
    <dbReference type="OMA" id="HKLMGAR"/>
    <dbReference type="OrthoDB" id="5982258at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000035">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000044">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000047">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000052">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000054">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000064">
        <property type="entry name" value="Contactin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000004">
        <property type="entry name" value="DCC isoform 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000005">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000028">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF18">
        <property type="entry name" value="CONTACTIN 3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043893685" evidence="15">
        <location>
            <begin position="19"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="684"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_053539700.1"/>
        </source>
    </evidence>
    <sequence length="1026" mass="111827" checksum="3CE062284C528BA2" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F3V8</accession>
    <name>A0A979F3V8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="24">Potassium voltage-gated channel subfamily KQT member 2 isoform X3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC108271553</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Cs(+)(in) = Cs(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78555"/>
            <dbReference type="ChEBI" id="CHEBI:49547"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Rb(+)(in) = Rb(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78547"/>
            <dbReference type="ChEBI" id="CHEBI:49847"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_047014590.1">
        <property type="nucleotide sequence ID" value="XM_047158634.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979F3V8"/>
    <dbReference type="GeneID" id="108271553"/>
    <dbReference type="CTD" id="100537363"/>
    <dbReference type="OrthoDB" id="8879391at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="potassium voltage-gated channel subfamily KQT member 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Putative potassium voltage-gated channel subfamily KQT member 2"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Ankyrin-G_BS"/>
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        <property type="entry name" value="Ion_trans_dom"/>
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        <property type="entry name" value="K_chnl_volt-dep_KCNQ"/>
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        <property type="entry name" value="K_chnl_volt-dep_KCNQ2"/>
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        <property type="entry name" value="K_chnl_volt-dep_KCNQ_C"/>
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        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 2"/>
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        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KCNQ2_u3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KCNQ_channel"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="KCNQC3-Ank-G_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
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        <property type="entry name" value="KCNQ2CHANNEL"/>
    </dbReference>
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        <property type="entry name" value="KCNQCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0630">Potassium</keyword>
    <keyword evidence="7" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="291"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="21">
        <location>
            <begin position="95"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel voltage dependent KCNQ C-terminal" evidence="22">
        <location>
            <begin position="440"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel voltage dependent KCNQ C-terminal" evidence="22">
        <location>
            <begin position="607"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="393"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="581"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="748"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="858"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="417"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="774"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="796"/>
            <end position="810"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044657"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF03520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_047014590.1"/>
        </source>
    </evidence>
    <sequence length="890" mass="98691" checksum="B284C2497DB06B30" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F859</accession>
    <name>A0A979F859_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="17">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23 24" type="primary">fynb</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23 24" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23 24">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="17"/>
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        <property type="nucleotide sequence ID" value="XM_047160281.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047016239.2">
        <property type="nucleotide sequence ID" value="XM_047160283.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979F859"/>
    <dbReference type="GeneID" id="108255503"/>
    <dbReference type="CTD" id="574422"/>
    <dbReference type="GeneTree" id="ENSGT00940000155462"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000553">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000016">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tyrosine-protein kinase Fyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 17 23" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0519">Myristate</keyword>
    <keyword evidence="7 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
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                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cystatin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017308726.2">
        <property type="nucleotide sequence ID" value="XM_017453237.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053530677.1">
        <property type="nucleotide sequence ID" value="XM_053674702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R045"/>
    <dbReference type="GeneID" id="128628985"/>
    <dbReference type="KEGG" id="ipu:108256406"/>
    <dbReference type="KEGG" id="ipu:128628985"/>
    <dbReference type="OrthoDB" id="2429551at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071220">
        <property type="term" value="P:cellular response to bacterial lipoprotein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.10:FF:000001">
        <property type="entry name" value="Cystatin-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018073">
        <property type="entry name" value="Prot_inh_cystat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001713">
        <property type="entry name" value="Prot_inh_stefin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11414:SF21">
        <property type="entry name" value="CYSTATIN 14A, TANDEM DUPLICATE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11414">
        <property type="entry name" value="CYSTATIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00031">
        <property type="entry name" value="Cystatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00295">
        <property type="entry name" value="STEFINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00043">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00287">
        <property type="entry name" value="CYSTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0789">Thiol protease inhibitor</keyword>
    <feature type="domain" description="Cystatin" evidence="9">
        <location>
            <begin position="3"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053530677.1"/>
        </source>
    </evidence>
    <sequence length="100" mass="11132" checksum="0A1D30921BBC1409" modified="2023-06-28" version="1">MSLLCGGTGEEQGATDEVQKICDEVKPHAEEKAGRTFDVFTAKSYKTQVVAGTNYFIKVHVGGNDFVHLRVYKMLPHAGEKLELHGIQTSKAHHDPIEYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R1Z5</accession>
    <name>A0A9F7R1Z5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histone H1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC128630393</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the histone H1/H5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053534894.1">
        <property type="nucleotide sequence ID" value="XM_053678919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R1Z5"/>
    <dbReference type="GeneID" id="128630393"/>
    <dbReference type="KEGG" id="ipu:128630393"/>
    <dbReference type="OrthoDB" id="8964560at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00073">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000075">
        <property type="entry name" value="Histone H1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005819">
        <property type="entry name" value="H1/H5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00624">
        <property type="entry name" value="HISTONEH5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H15" evidence="6">
        <location>
            <begin position="25"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="85"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="151"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053534894.1"/>
        </source>
    </evidence>
    <sequence length="198" mass="20231" checksum="70F5EA46A8479C1D" modified="2023-06-28" version="1">MAEVAPAPAPAKAPKKKAASRAKKAGPSVGELIVKAVSSSKERSGVSLAALKKALAAGGYDVEKNNSRVKIAVKGLVTKGTLVQTKGTGASGSFKLNKKQTEAKKPVKKAALKAKKPAATKKPKKVAAKKPAAAAKKSPKKAKKPTAAAKKATKSPKKAKKPATPKKAAKSPKKAKAVKPKTTKPKAAKAKKAAPKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R287</accession>
    <name>A0A9F7R287_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">zdhhc7</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="RefSeq" id="XP_053533811.1">
        <property type="nucleotide sequence ID" value="XM_053677836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R287"/>
    <dbReference type="GeneID" id="108264741"/>
    <dbReference type="CTD" id="55625"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF49">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="122"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053533811.1"/>
        </source>
    </evidence>
    <sequence length="251" mass="28464" checksum="3CE22D587A43B737" modified="2023-06-28" version="1">MQTSGHRLRDVEQHHPLLSSGEEEVTVGRVWFIQDGCGMVCASMTWFLVMYAEFVVNFVMLLPSKSFWYSLINGVAFNFLAVLALASHLRTMLTDPGAVPKGNATKEYMDSLQLKPGEVIYKCPKCCSIKPERAHHCSICKRCIRKMDHHCPWVNNCVGENNQRYFVLFTMYIASISLHALCLSGFHFFTCVKVQWNECSDFSPPVAVMLLIFLCLEALLFLTFTAVMFGTQIHSICNDETVSRTNQTPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RBE4</accession>
    <name>A0A9F7RBE4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">[histone H3]-trimethyl-L-lysine(9) demethylase</fullName>
            <ecNumber evidence="4">1.14.11.66</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">kdm4aa</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(9)-[histone H3] + 2 2-oxoglutarate + 2 O2 = N(6)-methyl-L-lysyl(9)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:60200"/>
            <dbReference type="Rhea" id="RHEA-COMP:15538"/>
            <dbReference type="Rhea" id="RHEA-COMP:15542"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="1.14.11.66"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the JHDM3 histone demethylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.66" evidence="4"/>
    <dbReference type="RefSeq" id="XP_053539680.1">
        <property type="nucleotide sequence ID" value="XM_053683705.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108271783"/>
    <dbReference type="KEGG" id="ipu:108271783"/>
    <dbReference type="CTD" id="100003413"/>
    <dbReference type="OrthoDB" id="9547406at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051864">
        <property type="term" value="F:histone H3K36 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140684">
        <property type="term" value="F:histone H3K9me2/H3K9me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20463">
        <property type="entry name" value="Tudor_JMJD2A_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20466">
        <property type="entry name" value="Tudor_JMJD2A_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000048">
        <property type="entry name" value="Lysine-specific demethylase 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000029">
        <property type="entry name" value="lysine-specific demethylase 4C isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.70:FF:000001">
        <property type="entry name" value="Putative lysine-specific demethylase 4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040477">
        <property type="entry name" value="KDM4-like_Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047479">
        <property type="entry name" value="Tudor_KDM4A_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047481">
        <property type="entry name" value="Tudor_KDM4A_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF51">
        <property type="entry name" value="[HISTONE H3]-TRIMETHYL-L-LYSINE(9) DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13831">
        <property type="entry name" value="PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18104">
        <property type="entry name" value="Tudor_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="10" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjN" evidence="18">
        <location>
            <begin position="14"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="19">
        <location>
            <begin position="142"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="20">
        <location>
            <begin position="1834"/>
            <end position="1947"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="350"/>
            <end position="1611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1627"/>
            <end position="1659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="374"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="419"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="512"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="534"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="595"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="640"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="662"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="693"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="705"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="773"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="787"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="831"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="915"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="942"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1011"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1040"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1055"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1073"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
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            <end position="1102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1109"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1127"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1145"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1163"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1181"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1199"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1222"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1253"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1289"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1307"/>
            <end position="1327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1350"/>
            <end position="1369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="1376"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1435"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1465"/>
            <end position="1497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1501"/>
            <end position="1512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1531"/>
            <end position="1561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1577"/>
            <end position="1587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1631"/>
            <end position="1643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_053539680.1"/>
        </source>
    </evidence>
    <sequence length="2126" mass="239249" checksum="B2FAB156FB89EC1A" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RCS9</accession>
    <name>A0A9F7RCS9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11 12">NACHT, LRR and PYD domains-containing protein 1a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 11 12" type="primary">LOC108259949</name>
    </gene>
    <organism evidence="9 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11 12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11 12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053533005.1">
        <property type="nucleotide sequence ID" value="XM_053677030.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053533006.1">
        <property type="nucleotide sequence ID" value="XM_053677031.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053533007.1">
        <property type="nucleotide sequence ID" value="XM_053677032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RCS9"/>
    <dbReference type="GeneID" id="108259949"/>
    <dbReference type="KEGG" id="ipu:108259949"/>
    <dbReference type="OrthoDB" id="8891580at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08330">
        <property type="entry name" value="CARD_ASC_NALP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
        <property type="entry name" value="CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033516">
        <property type="entry name" value="CARD8/ASC/NALP1_CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025307">
        <property type="entry name" value="FIIND_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051249">
        <property type="entry name" value="NLRP_Inflammasome"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46985:SF2">
        <property type="entry name" value="APOPTOSIS-ASSOCIATED SPECK-LIKE PROTEIN CONTAINING A CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46985">
        <property type="entry name" value="NACHT, LRR AND PYD DOMAINS-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00619">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13553">
        <property type="entry name" value="FIIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23679">
        <property type="entry name" value="UPA-FIIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51830">
        <property type="entry name" value="FIIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FIIND" evidence="8">
        <location>
            <begin position="35"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="CARD" evidence="7">
        <location>
            <begin position="313"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053533005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053533006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053533007.1"/>
        </source>
    </evidence>
    <sequence length="397" mass="45354" checksum="A6066AB12748A9C6" modified="2023-06-28" version="1">MKKSALLTEDFREPIGTGKGAQSTSTLHPDTGHVSADTEIFTPELVDNYPNDKNRMKYRFFCSRAGQFKCKLTDLVFEMEGKGEVLYRIVSWDSCVSDGLQKDSAGPLYSIECLKGSIRHLHLPHCETRTDVVKLTVAHATGGNVEIIQPLKVTDTHVIIHIHGLSLFGILKALLFQEYPIRAQVLLFRKKMTRKLSRSKLNIHLLPGNVPVKEVQKQRECNEYIETTSKCQLSPGKLYRLCCKTTDRDYKPQPEEETFDLDYGPNYHPTFEVLFNTELDEITLSLLDENGQQVWTPREVSLLTDTEAVSANTVKAGADFVDKHRETLIQRVPSVTEIADVLKGKKMISDEMYYKIKSKEIGQEQMRELYRCLTSTAVKAEFYKILQEKHQYILEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RFI4</accession>
    <name>A0A9F7RFI4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="1">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">LOC128629736</name>
        <name evidence="6" type="synonym">LOC128630148</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5 6" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4 5 6">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="1"/>
    <dbReference type="RefSeq" id="XP_053534462.1">
        <property type="nucleotide sequence ID" value="XM_053678487.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534463.1">
        <property type="nucleotide sequence ID" value="XM_053678488.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534740.1">
        <property type="nucleotide sequence ID" value="XM_053678765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RFI4"/>
    <dbReference type="GeneID" id="128629736"/>
    <dbReference type="KEGG" id="ipu:128629736"/>
    <dbReference type="OrthoDB" id="26387at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF28">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053534462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053534463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_053534740.1"/>
        </source>
    </evidence>
    <sequence length="147" mass="16634" checksum="2376576874CDF50A" modified="2023-06-28" version="1">MTRIDQEAEEAQRKLKRQNGEDPALPPFCPLFASLGNILQCDVLLGMLGAVLQWAMEPSGGHWSESMLQRVLHLIGMALLEEQQQLENIGDDDEVTFNFTLKISPCMKQCCTEQMTECLDWIMAECKNLYSKFLQSMAISFIQKSDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RGF7</accession>
    <name>A0A9F7RGF7_ICTPU</name>
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        <recommendedName>
            <fullName evidence="15">Neuron navigator 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Pore membrane and/or filament-interacting-like protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20 21" type="primary">nav1b</name>
    </gene>
    <organism evidence="19 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20 21" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20 21">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">May be involved in neuronal migration.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with tubulin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nav/unc-53 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053539706.1">
        <property type="nucleotide sequence ID" value="XM_053683731.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053539707.1">
        <property type="nucleotide sequence ID" value="XM_053683732.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108271926"/>
    <dbReference type="CTD" id="100005000"/>
    <dbReference type="OrthoDB" id="2161974at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043194">
        <property type="term" value="C:axon initial segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001578">
        <property type="term" value="P:microtubule bundle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000409">
        <property type="entry name" value="Neuron navigator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057568">
        <property type="entry name" value="CortBP2_NAV1-like_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039041">
        <property type="entry name" value="Nav/unc-53"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057126">
        <property type="entry name" value="NAV1-like_ubiquitin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12784:SF3">
        <property type="entry name" value="NEURON NAVIGATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12784">
        <property type="entry name" value="STEERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25408">
        <property type="entry name" value="AAA_lid_NAV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23092">
        <property type="entry name" value="Ubiquitin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="18">
        <location>
            <begin position="1553"/>
            <end position="1692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="202"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
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            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="576"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
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            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
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            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="931"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1256"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1357"/>
            <end position="1381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1814"/>
            <end position="1850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="22"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="73"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="210"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="315"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="344"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="420"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="443"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="589"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="639"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="663"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="742"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="777"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="798"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="939"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="981"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1016"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1363"/>
            <end position="1381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00064590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00067341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00080430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_053539706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_053539707.1"/>
        </source>
    </evidence>
    <sequence length="1877" mass="204349" checksum="D15B4F9DABE7F6D6" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RIH3</accession>
    <name>A0A9F7RIH3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Myosin-binding protein C, fast-type</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">C-protein, skeletal muscle fast isoform</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">mybpc2b</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the immunoglobulin superfamily. MyBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053540862.1">
        <property type="nucleotide sequence ID" value="XM_053684887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RIH3"/>
    <dbReference type="GeneID" id="108272997"/>
    <dbReference type="KEGG" id="ipu:108272997"/>
    <dbReference type="CTD" id="541384"/>
    <dbReference type="OrthoDB" id="6107607at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05894">
        <property type="entry name" value="Ig_C5_MyBP-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000646">
        <property type="entry name" value="Myosin binding protein C, fast type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000225">
        <property type="entry name" value="Myosin-binding protein C, cardiac-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000326">
        <property type="entry name" value="Myosin-binding protein C, cardiac-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000031">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000060">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000062">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000070">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000081">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000085">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000111">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040849">
        <property type="entry name" value="MyBP-C_THB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF43">
        <property type="entry name" value="MYOSIN-BINDING PROTEIN C, FAST-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18362">
        <property type="entry name" value="THB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="245"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="413"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="515"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="618"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="716"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="14">
        <location>
            <begin position="912"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="1025"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00053486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_053540862.1"/>
        </source>
    </evidence>
    <sequence length="1123" mass="126324" checksum="6D730E2B1B9DD189" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RPA6</accession>
    <name>A0A9F7RPA6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">STE20-related kinase adapter protein beta isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">stradb</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053539472.1">
        <property type="nucleotide sequence ID" value="XM_053683497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RPA6"/>
    <dbReference type="GeneID" id="108271608"/>
    <dbReference type="KEGG" id="ipu:108271608"/>
    <dbReference type="CTD" id="55437"/>
    <dbReference type="OrthoDB" id="840771at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902554">
        <property type="term" value="C:serine/threonine protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047173">
        <property type="entry name" value="STRAD_A/B-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48014">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE FRAY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48014:SF13">
        <property type="entry name" value="STE20-RELATED KINASE ADAPTER PROTEIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="55"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="403"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_053539472.1"/>
        </source>
    </evidence>
    <sequence length="422" mass="46068" checksum="9E3E6AF9863FCACC" modified="2023-06-28" version="1">MSFLDCSCISAHSQVQPISCEDQYEDISGSYLSSDGSFFGADYDLSGLSADPADYQLLSLLGRGFNNLSQVSMARHRPSGRLVAVKNTNLDECTEEELLQLMNEALLSRLFRHPNLLTSRLVFSSCCQLWVLSPLMTYGSADSLLRSFFPDGMSESLVAYLLYGVLNALKYLHHMGYIHRGVKASHVLLSAGGHVCLSGLQCVYSLMRDGRRMEVVFDMPQHTPSLLPWLSPELLRQDLHGYGVKSDIYSVGVLALELVSGRVPFQDLPPTLMLLQKLRGSLSCMLDGAPYALGDFSGLKVSRSGVDSGIGESVAAGSLTRTATAERPQSPAPKNHSATLHNLLQLCLQQQPERRPSASALLSHPFFKQVKKHTSDSFLSLVYPAIPLSCPVDVPLSPPLLHTDTHTDANPEPADGGVWDFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RPY8</accession>
    <name>A0A9F7RPY8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Myosin light polypeptide 6 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">myl6</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053539738.1">
        <property type="nucleotide sequence ID" value="XM_053683763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RPY8"/>
    <dbReference type="GeneID" id="108271738"/>
    <dbReference type="KEGG" id="ipu:108271738"/>
    <dbReference type="CTD" id="4637"/>
    <dbReference type="OrthoDB" id="5959761at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008307">
        <property type="term" value="F:structural constituent of muscle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000019">
        <property type="entry name" value="Myosin light chain 1 skeletal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000056">
        <property type="entry name" value="Myosin light chain 1 skeletal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF7">
        <property type="entry name" value="SIMILAR TO MYOSIN, LIGHT POLYPEPTIDE 6, ALKALI, SMOOTH MUSCLE AND NON-MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="1" id="KW-0518">Myosin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="7"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="84"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_053539738.1"/>
        </source>
    </evidence>
    <sequence length="151" mass="16908" checksum="41A10FACA5729DE7" modified="2023-06-28" version="1">MSEFSEDQILEFKEAFLLFDRTGDGKISYSQCGDVMRALGQNPVNAEVLKVLGNPKAEEMNMKMLDFEQFLPMLQAIAKNKDQGSFEDFVEGLRVFDKEGNGTVMGAELRHVLTTLGEKMTEEEVETLLAGHEDANGCINYEELVRMVMSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RT28</accession>
    <name>A0A9F7RT28_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcriptional adapter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">tada2a</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053543343.1">
        <property type="nucleotide sequence ID" value="XM_053687368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RT28"/>
    <dbReference type="GeneID" id="108278301"/>
    <dbReference type="CTD" id="6871"/>
    <dbReference type="OrthoDB" id="270417at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140672">
        <property type="term" value="C:ATAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051302">
        <property type="term" value="P:regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02335">
        <property type="entry name" value="ZZ_ADA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000110">
        <property type="entry name" value="Transcriptional adapter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000020">
        <property type="entry name" value="Transcriptional adapter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000087">
        <property type="entry name" value="Transcriptional adapter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041983">
        <property type="entry name" value="ADA2-like_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016827">
        <property type="entry name" value="Ada2/TADA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055141">
        <property type="entry name" value="TADA2A_B-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374:SF20">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 2-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTOR 2 ADA2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22941">
        <property type="entry name" value="TADA2A-like_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25299">
        <property type="entry name" value="ZZ_ADA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025024">
        <property type="entry name" value="Transcriptional_adaptor_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="14">
        <location>
            <begin position="28"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="16">
        <location>
            <begin position="86"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="13">
        <location>
            <begin position="91"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="SWIRM" evidence="15">
        <location>
            <begin position="372"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR025024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_053543343.1"/>
        </source>
    </evidence>
    <sequence length="459" mass="53550" checksum="96DB71695CAA631D" modified="2023-06-28" version="1">MDVNHCRTTMDQISFQRGSIDTDADDPFDKPPCRGCSSYLVEPYVKCAECGPSPFVLCLQCFTRGYEYKKHQSDHKYEIMTSDFPILEPGWTAQEEMALLEAVMDCGFGNWQDVAYQMRTKTKEECEGHYMKNFINNPLFSSTLLSLRQREEARSPDTAIPFKPTDDPPRPSFDSQLSRDMAGYMPARADFMEEFDNYAEWDLKDIDFVDDDSDILHALKIAVVDIYHSRLKERQRRKRIIRDHGLINLRKFQILERRYPKVVQDLYDAMRRFARVVGPLEHDKFIESHALEFELRREIRRLQEYRKEGIQSFCGAKVYEQVKQAREDERNKRNMLCEVLQYIHDSRACQQWLHKQAAIDAGITPIITTITTSGRRSAPPLNLTGLPGTEKLNEREKELCQVVRLVPGAYLEYKQALLNECQRQGGLRLAQARSLIKIDVNKTRKIYDFLIKEGYINKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RTP7</accession>
    <name>A0A9F7RTP7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13">Galactosylceramide sulfotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">gal3st1b</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12 13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the galactose-3-O-sulfotransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053542907.1">
        <property type="nucleotide sequence ID" value="XM_053686932.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053542908.1">
        <property type="nucleotide sequence ID" value="XM_053686933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RTP7"/>
    <dbReference type="GeneID" id="108277025"/>
    <dbReference type="KEGG" id="ipu:108277025"/>
    <dbReference type="CTD" id="793584"/>
    <dbReference type="GeneTree" id="ENSGT00950000182923"/>
    <dbReference type="OMA" id="FYSPEAF"/>
    <dbReference type="OrthoDB" id="514299at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001733">
        <property type="term" value="F:galactosylceramide sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006682">
        <property type="term" value="P:galactosylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042552">
        <property type="term" value="P:myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000807">
        <property type="entry name" value="galactosylceramide sulfotransferase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009729">
        <property type="entry name" value="Gal-3-0_sulfotransfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14647">
        <property type="entry name" value="GALACTOSE-3-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14647:SF56">
        <property type="entry name" value="GALACTOSYLCERAMIDE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06990">
        <property type="entry name" value="Gal-3-0_sulfotr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044698461" evidence="10">
        <location>
            <begin position="31"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053542907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_053542908.1"/>
        </source>
    </evidence>
    <sequence length="420" mass="48837" checksum="49925C49B1F16F36" modified="2023-06-28" version="1">MFHHLPRSGAHKLMLGFLFTCITMMLYCFSTPTFQGNTPRFPVPAPCANKTPPSQKNKGSFSSSEVKHCVPKVNLMFLKTHKAASSTILNILLRYGEKQRLTFALPNGRNDFAYPATFTRTHVKDYRPGACFNIICNHMRFNAPEVEALLPADAFFFTILRDPALVFESSFHYFKRVVPLIWRIHGEDKLTEFLNDPQLYFNSEGFNSFYLKNPHFFDLGFDNTLEPEDPTVDSAIHAIAERFQLVLIAEHFEESLILLKDALCWQMDDLLFFNMNTRRPSSVSQLTPELRVKAREWNSVDWKLYRYFNATLWAKVDAYGRERMQKEVKKLRQRNSEMAAICISGGMAVEDQDIRDQSMRPWQPVGESSILGYNMKSNIEPQYRELCRKMLTPEIQYLTDLGVNLWITRLWGWFKAFLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TNV5</accession>
    <name>A0A9F7TNV5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutamine synthetase</fullName>
            <ecNumber evidence="5">6.3.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Glutamate--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC108271536</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14 15">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="5"/>
    <dbReference type="RefSeq" id="XP_053539719.1">
        <property type="nucleotide sequence ID" value="XM_053683744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TNV5"/>
    <dbReference type="GeneID" id="108271536"/>
    <dbReference type="OrthoDB" id="1936100at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.70:FF:000004">
        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000011">
        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050292">
        <property type="entry name" value="Glutamine_Synthetase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852:SF45">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS beta-grasp" evidence="16">
        <location>
            <begin position="23"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="GS catalytic" evidence="17">
        <location>
            <begin position="112"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_053539719.1"/>
        </source>
    </evidence>
    <sequence length="379" mass="43105" checksum="35AF547AE71C8076" modified="2023-06-28" version="1">MATSSSKLNTAVTQWYMDLPQGDKVQAMYIWIDGTGEGLRCKTRTLDSEPKTIEDLPEWNFGGTSIFPVERSNSDMYLIPKAIFRDPFRKDPNKLVLCEVLKYNRKPAVTNLRQMCDKIMSMVEDQHPWFGMEQKYTLLAIDGHPFRGPSNGFPGPQGPYYCGVGADKACGRDIVEAHYRACLYAGVMICGTNAEVMPVQWEFQVCPCEGISMGDHLWIARFILHRVCEGFGVVASFDPKLITGNWNGAGCHTNFSTKNMREEGGLRHVEEAIERLGKRHHYHIRAYDPKGGLDNVRRLTGHHETSNINEFSEGYFEDRRGLDNVRRLTGHHETSNINEFSEGYFEDRRPSANCDPYAVTEAIIRTCLLNEDEEPTVYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3TE90</accession>
    <name>E3TE90_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Leukocyte elastase inhibitor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Serpin B1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ILEU</name>
        <name evidence="12" type="synonym">ileu</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the serpin family. Ov-serpin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GU588670">
        <property type="protein sequence ID" value="ADO28626.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187426.1">
        <property type="nucleotide sequence ID" value="NM_001200497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TE90"/>
    <dbReference type="MEROPS" id="I04.006"/>
    <dbReference type="GeneID" id="100528227"/>
    <dbReference type="KEGG" id="ipu:100528227"/>
    <dbReference type="CTD" id="100528227"/>
    <dbReference type="OrthoDB" id="671595at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.497.10:FF:000001">
        <property type="entry name" value="Serine protease inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.39.10:FF:000014">
        <property type="entry name" value="Serpin family B member 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF180">
        <property type="entry name" value="LEUKOCYTE ELASTASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0722">Serine protease inhibitor</keyword>
    <feature type="domain" description="Serpin" evidence="9">
        <location>
            <begin position="13"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00079383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADO28626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="NP_001187426.1"/>
        </source>
    </evidence>
    <sequence length="380" mass="42950" checksum="C6845DF16BECA96E" modified="2011-01-11" version="1">MESLSRANSAFALDLYRAISAENPDGNLFFSPLSVSAALSMVYLGARGTTADEMAKVLSFSSIPDVHTHFKTLNSAINSPKASYILRLANRLYGEKTFSFSSEYVSSTLKLYQADMQAVDFIGASEESRKLVNHWVEEKTEGKIKDILQSGTVTAMTRLALVNAIYFKGKWKNVFNTEHTKEMPFKINQNETKPVQMMYQTKKFLFNYVDEYRLQVLDLPYVEEELSMVVLLPQESEDGSDPLQKLECELTVDKLLEWTNQEKMDRWTDIIVHLPKFKLEEQYSLKDILNKMGMSSLFQASAADLTGMSSKGGLFVSSVTHKAFVEVNEEGTEAAAATAVMVNFCMARGEHFMADHPCLFFIRHNPTKSILFFGKYRGPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-03-07" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>Q2HYN5</accession>
    <name>Q2HYN5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8 10">Eukaryotic translation initiation factor 1</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="7">Translation factor sui1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">eif1</name>
        <name evidence="8" type="synonym">EIF1B</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2006-01" db="EMBL/GenBank/DDBJ databases">
            <title>Channel catfish gene expression after Edwardsiella ictaluri infection.</title>
            <authorList>
                <person name="Yeh H.-Y."/>
                <person name="Klesius P.H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">ATCC CRL-2772</strain>
            <tissue evidence="7">Ovary</tissue>
        </source>
    </reference>
    <reference evidence="8 10" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SUI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DQ363457">
        <property type="protein sequence ID" value="ABC94624.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JT419273">
        <property type="protein sequence ID" value="AHH43012.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001232871.1">
        <property type="nucleotide sequence ID" value="NM_001245942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q2HYN5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020254"/>
    <dbReference type="GeneID" id="100304735"/>
    <dbReference type="KEGG" id="ipu:100304735"/>
    <dbReference type="CTD" id="10209"/>
    <dbReference type="GeneTree" id="ENSGT00390000015789"/>
    <dbReference type="OMA" id="VENHIHI"/>
    <dbReference type="OrthoDB" id="10248435at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11566">
        <property type="entry name" value="eIF1_SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.780.10:FF:000003">
        <property type="entry name" value="Eukaryotic translation initiation factor 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005874">
        <property type="entry name" value="SUI1_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01160">
        <property type="entry name" value="SUI1_MOF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10388">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004499">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2 8 10" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="31"/>
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        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00005422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABC94624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AHH43012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="NP_001232871.1"/>
        </source>
    </evidence>
    <sequence length="113" mass="12897" checksum="1D13F790FE2F1C7B" modified="2006-03-07" version="1">MSAIQNLQTFDPFADATKGDDRLPAGTEDYIHIRIQQRNGRKTLTTVQGIADDYDKKKLVKAFKKKFACNGTVIEHPEYGEVIQLQGDQRKNICQFLTEIELAKEEQLKVHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-03-15" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>Q5EG09</accession>
    <name>Q5EG09_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Leukocyte immune-type receptor 1</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="8">Leukocyte immune-type receptor TS32.15 L2.2a precursor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC100379168</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2005-01" db="EMBL/GenBank/DDBJ databases">
            <title>Characterization of leukocyte immune-type receptors in catfish.</title>
            <authorList>
                <person name="Stafford J.L."/>
                <person name="Bengten E."/>
                <person name="Wilson M."/>
                <person name="McIntosh R.D."/>
                <person name="Quiniou S."/>
                <person name="Miller N.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2007" name="Immunogenetics" volume="59" first="77" last="91">
            <title>Channel catfish leukocyte immune-type receptors contain a putative MHC class I binding site.</title>
            <authorList>
                <person name="Stafford J.L."/>
                <person name="Bengten E."/>
                <person name="Du Pasquier L."/>
                <person name="Miller N.W."/>
                <person name="Wilson M."/>
            </authorList>
            <dbReference type="PubMed" id="17149620"/>
            <dbReference type="DOI" id="10.1007/s00251-006-0169-3"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="journal article" date="2016" name="J. Immunol." volume="196" first="2677" last="2689">
            <title>A Leukocyte Immune-Type Receptor Subset Is a Marker of Antiviral Cytotoxic Cells in Channel Catfish, Ictalurus punctatus.</title>
            <authorList>
                <person name="Taylor E.B."/>
                <person name="Moulana M."/>
                <person name="Stuge T.B."/>
                <person name="Quiniou S.M."/>
                <person name="Bengten E."/>
                <person name="Wilson M."/>
            </authorList>
            <dbReference type="PubMed" id="26856701"/>
            <dbReference type="DOI" id="10.4049/jimmunol.1502166"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="5">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="AY885643">
        <property type="protein sequence ID" value="AAW82352.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001316173.1">
        <property type="nucleotide sequence ID" value="NM_001329244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q5EG09"/>
    <dbReference type="GeneID" id="100379168"/>
    <dbReference type="KEGG" id="ipu:100379168"/>
    <dbReference type="OrthoDB" id="6151406at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001607">
        <property type="entry name" value="Leukocyte immune-type receptor TS32.15 L2.5a"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050488">
        <property type="entry name" value="Ig_Fc_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481:SF112">
        <property type="entry name" value="FC RECEPTOR-LIKE PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481">
        <property type="entry name" value="IMMUNOGLOBULIN FC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_5035982254" evidence="8">
        <location>
            <begin position="14"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="364"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="13"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="96"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="189"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="271"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="396"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="468"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="400"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="483"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAW82352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="NP_001316173.1"/>
        </source>
    </evidence>
    <sequence length="496" mass="55905" checksum="2C9A821045F1EEB8" modified="2005-03-15" version="1">MILLISLIRVGQAQVLSVKPNSPQIFRGETVTLTCRISGGNGLYYWYRDGFNVHRSAENYYTIKVDQSHKYRCYGTIYGRTTAWSNEVTLSVIERPKAVLTLQPDGQIFSGQEVTFTCEIRGHADTEWMYNWNKDGVQISSYTESRKYSFTPVESLSAKYTCSGRRRSDSQTSETSNTVTLTVSEKPKPTVRVNPQSSVYTGDRVTLNCNLLSNGWTFLWYKDRVQVAEDTNTLHDTVSNAGETVYQCKARRGNYDSELSDPVTLTVRALPKPVVSINPDEQVYRGETVTLRCDLQDEEDSDWIYSWNKDGSGVSSEQEYRISSAETSHAGKYTCRGRERGGSHWSHTSDAVTLTVSGSTYNTVIVFVSVGLSLAFLFIILLLILCWWHKSNKGKDRDIQQNSNQTPGQNPSQSGAEDSQSGHAPLQTDDDAGPSDVIYTELEIKPQKKAKKNQVKVSVEYGTVYSQLKQRHRSGRSEYTTEQTREKRERSSSCVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2003-10-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q7T0B3</accession>
    <name>Q7T0B3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13 14">C-X-C motif chemokine 11-6</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="11">Chemokine</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CXCL10</name>
        <name evidence="13" type="synonym">LOC128628743</name>
        <name evidence="14" type="synonym">LOC128628744</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2004" name="Dev. Comp. Immunol." volume="28" first="769" last="780">
            <title>Sequence analysis and expression of a CXC chemokine in resistant and susceptible catfish after infection of Edwardsiella ictaluri.</title>
            <authorList>
                <person name="Baoprasertkul P."/>
                <person name="Peatman E."/>
                <person name="Chen L."/>
                <person name="He C."/>
                <person name="Kucuktas H."/>
                <person name="Li P."/>
                <person name="Simmons M."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="15043945"/>
            <dbReference type="DOI" id="10.1016/j.dci.2003.12.002"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13 14" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13 14">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Ligand for cxcr3.2. Chemotactic for macrophages.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the intercrine alpha (chemokine CxC) family.</text>
    </comment>
    <dbReference type="EMBL" id="AY335950">
        <property type="protein sequence ID" value="AAQ01586.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053530586.1">
        <property type="nucleotide sequence ID" value="XM_053674611.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053530599.1">
        <property type="nucleotide sequence ID" value="XM_053674624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q7T0B3"/>
    <dbReference type="KEGG" id="ipu:128628743"/>
    <dbReference type="KEGG" id="ipu:128628744"/>
    <dbReference type="GeneTree" id="ENSGT01030000234997"/>
    <dbReference type="OrthoDB" id="8872899at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042056">
        <property type="term" value="F:chemoattractant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00273">
        <property type="entry name" value="Chemokine_CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000004">
        <property type="entry name" value="C-X-C motif chemokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039809">
        <property type="entry name" value="Chemokine_b/g/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001089">
        <property type="entry name" value="Chemokine_CXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033899">
        <property type="entry name" value="CXC_Chemokine_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015:SF191">
        <property type="entry name" value="C-X-C MOTIF CHEMOKINE 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015">
        <property type="entry name" value="SMALL INDUCIBLE CYTOKINE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00436">
        <property type="entry name" value="INTERLEUKIN8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00437">
        <property type="entry name" value="SMALLCYTKCXC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00199">
        <property type="entry name" value="SCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="4" id="KW-0202">Cytokine</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044738070" evidence="9">
        <location>
            <begin position="21"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="10">
        <location>
            <begin position="26"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAQ01586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_053530586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_053530599.1"/>
        </source>
    </evidence>
    <sequence length="95" mass="10340" checksum="EB5CF92D3070DD58" modified="2003-10-01" version="1">MKSAAVFVVFACLLIVHVQGQARTSVRRCLCQGPAANGVRLQRIDKIEIHPASATCENKEIIVTLKNGAGKKCLNPESEFTKKYITAALEKRSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-10-01" modified="2026-01-28" version="107" xmlns="https://uniprot.org/uniprot">
    <accession>Q8JG37</accession>
    <name>Q8JG37_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tumor necrosis factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">TNF-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TNF</name>
        <name evidence="15" type="synonym">tnf</name>
        <name evidence="15" type="synonym">tnfa</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2003-02" db="EMBL/GenBank/DDBJ databases">
            <title>Cloning and expression of a tumor necrosis factor homologue in catfish, Ictalurus punctatus.</title>
            <authorList>
                <person name="Zou J."/>
                <person name="Chinchar G."/>
                <person name="Long S."/>
                <person name="Miller N."/>
                <person name="Secombes C.J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tumor necrosis factor family.</text>
    </comment>
    <dbReference type="EMBL" id="AJ417565">
        <property type="protein sequence ID" value="CAD10389.2"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187101.1">
        <property type="nucleotide sequence ID" value="NM_001200172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8JG37"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000023721"/>
    <dbReference type="GeneID" id="100304592"/>
    <dbReference type="KEGG" id="ipu:100304592"/>
    <dbReference type="CTD" id="405785"/>
    <dbReference type="OrthoDB" id="9940698at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005164">
        <property type="term" value="F:tumor necrosis factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00184">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006053">
        <property type="entry name" value="TNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006052">
        <property type="entry name" value="TNF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11471:SF23">
        <property type="entry name" value="TUMOR NECROSIS FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11471">
        <property type="entry name" value="TUMOR NECROSIS FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00229">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01234">
        <property type="entry name" value="TNECROSISFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00207">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50049">
        <property type="entry name" value="THD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0202">Cytokine</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="THD" evidence="12">
        <location>
            <begin position="78"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="CAD10389.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="NP_001187101.1"/>
        </source>
    </evidence>
    <sequence length="230" mass="25598" checksum="3A40D391F75DB019" modified="2003-06-01" version="2">MASDSQVVLDVDGPRVTIVREKASWSSSGVWRTCGVLLAVALCAAAAVCFSQNKTHNKPDETQEIKHSLRQISQTAKAAIHLSGHYNPQVSSVSMQWFDNADQSFSSGLKLEDNEIKILRDGLYFVYSQASYRLLCKAEGDETEGEVMHMSHKVSRWSDSYSSWKPLLSATRSACKKTTEEYQKYWYGAVYLGAAFNLKAGDRLRTVMDEKLLPKVESAGGKTFFGTFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5U8B7</accession>
    <name>W5U8B7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Beta-1,3-N-acetylglucosaminyltransferase</fullName>
            <ecNumber evidence="18">2.4.1.222</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25 27" type="primary">rfng</name>
    </gene>
    <organism evidence="25">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="25">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">SDA103</strain>
        </source>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="27">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 18">
            <text>3-O-(alpha-L-fucosyl)-L-seryl-[EGF-like domain protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-alpha-L-fucosyl)-L-seryl-[EGF-like domain protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70511"/>
            <dbReference type="Rhea" id="RHEA-COMP:17919"/>
            <dbReference type="Rhea" id="RHEA-COMP:17920"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:189632"/>
            <dbReference type="ChEBI" id="CHEBI:189633"/>
            <dbReference type="EC" id="2.4.1.222"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 18">
            <text>3-O-(alpha-L-fucosyl)-L-threonyl-[EGF-like domain protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-alpha-L-fucosyl)-L-threonyl-[EGF-like domain protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70531"/>
            <dbReference type="Rhea" id="RHEA-COMP:17922"/>
            <dbReference type="Rhea" id="RHEA-COMP:17923"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:189631"/>
            <dbReference type="ChEBI" id="CHEBI:189634"/>
            <dbReference type="EC" id="2.4.1.222"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18 21">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.222" evidence="18"/>
    <dbReference type="EMBL" id="JT407984">
        <property type="protein sequence ID" value="AHH38198.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JT418331">
        <property type="protein sequence ID" value="AHH42700.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017338494.1">
        <property type="nucleotide sequence ID" value="XM_017483005.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U8B7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000025486"/>
    <dbReference type="GeneID" id="108273644"/>
    <dbReference type="KEGG" id="ipu:108273644"/>
    <dbReference type="CTD" id="5986"/>
    <dbReference type="OMA" id="DTDWCPM"/>
    <dbReference type="OrthoDB" id="8959630at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033829">
        <property type="term" value="F:O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007389">
        <property type="term" value="P:pattern specification process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000003">
        <property type="entry name" value="Beta-1,3-N-acetylglucosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017374">
        <property type="entry name" value="Fringe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003378">
        <property type="entry name" value="Fringe-like_glycosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10811">
        <property type="entry name" value="FRINGE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02434">
        <property type="entry name" value="Fringe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038073">
        <property type="entry name" value="B-acetylgalactosaminyltfrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 18 25" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15 18 21" id="KW-0464">Manganese</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 18 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="23" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 18 25" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540100" description="Beta-1,3-N-acetylglucosaminyltransferase" evidence="23">
        <location>
            <begin position="32"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Fringe-like glycosyltransferase" evidence="24">
        <location>
            <begin position="95"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="active site" evidence="19">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR038073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR038073-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR038073-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR038073-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF02434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="AHH38198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_017338494.1"/>
        </source>
    </evidence>
    <sequence length="366" mass="41192" checksum="4861CE443DE580C1" modified="2014-04-16" version="1">MHLSPVGTNRRCFLASSALCAIVLLLIPTLQTPQQQGELPRPLPHNRPARGDNTTGYKGASNAKHSRVIGLPFPRDKSQRDSIGAEILSSQSKEPLRLKDIFIAVKTTRKYHKSRLELLFQTWVSQAKEQTFIFTDGEDKELKQKAEGNVINTNCSAAHTRQALCCKMSVEYDKFIESQKRWFCHVDDDNYVILPSLLQLLSSYSHTQDVYLGRPSLDHPIEAPERVRSDGSTSVRFWFATGGAGFCVSRGLALKMSPWASLGNFITTAEKIRLPDDCTIGYIIEALLEVPLIHTNLFHSHLENLQRMPAADVLKQVTLSYGGFENRRNVIPIAGAFSLTEDPSRFKTVHCLLYPDTEWCLRNGHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>W5U8S4</accession>
    <accession>A0A2D0RKC3</accession>
    <name>W5U8S4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7 9">Transmembrane protein 179</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tmem179</name>
        <name evidence="9" type="synonym">LOC108269529</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TMEM179 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT408456">
        <property type="protein sequence ID" value="AHH38343.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017330963.2">
        <property type="nucleotide sequence ID" value="XM_017475474.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U8S4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000929"/>
    <dbReference type="GeneID" id="108269529"/>
    <dbReference type="KEGG" id="ipu:108269529"/>
    <dbReference type="GeneTree" id="ENSGT00510000048283"/>
    <dbReference type="OrthoDB" id="6423876at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029673">
        <property type="entry name" value="TMEM179"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059010">
        <property type="entry name" value="TMEM179-179B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31872">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31872:SF5">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26158">
        <property type="entry name" value="Claudin_TMEM179-179B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00093776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AHH38343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017330963.2"/>
        </source>
    </evidence>
    <sequence length="233" mass="26415" checksum="150BCCCB4D937D1C" modified="2014-04-16" version="1">MAKNNFIFTQCVLYFLAFVFGFIAVVPLSEYAEDFGGKCLLFTRGMWQNENITVSKQRFMVEEWGPQSSCSFITFVGVASLVLAAVQAWRLLFLICKGHDDSIFNAFLNLLISLFVVCAVFLSSTIVTVGFNLWCDAITEGGSMPSSCEELQDTDLELGLDNSAFYDQFAIAQFGLWAAWLTWLGITFMAFLKVYYNYRQEDLLDSLIHEKEFLLGRSSRRCSDILDKKSSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5U8U7</accession>
    <name>W5U8U7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin-conjugating enzyme E2Q-like protein 1</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">E2Q-like ubiquitin-conjugating enzyme 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Ube2ql1</name>
        <name evidence="14" type="synonym">ube2ql1</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Probable E2 ubiquitin-protein ligase that catalyzes the covalent attachment of ubiquitin to target proteins. May facilitate the monoubiquitination and degradation of MTOR and CCNE1 through interaction with FBXW7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with FBXW7.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="JT408634">
        <property type="protein sequence ID" value="AHH38411.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017337999.1">
        <property type="nucleotide sequence ID" value="XM_017482510.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U8U7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021879"/>
    <dbReference type="GeneID" id="108273343"/>
    <dbReference type="KEGG" id="ipu:108273343"/>
    <dbReference type="CTD" id="134111"/>
    <dbReference type="OMA" id="TCTEFIL"/>
    <dbReference type="OrthoDB" id="109543at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23802">
        <property type="entry name" value="UBCc_UBE2Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000036">
        <property type="entry name" value="ubiquitin-conjugating enzyme E2Q-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="11">
        <location>
            <begin position="105"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00065076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00080249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AHH38411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017337999.1"/>
        </source>
    </evidence>
    <sequence length="271" mass="30541" checksum="1C9427F45391E22D" modified="2014-04-16" version="1">MATLLRKIGLIRLHDRDTEDPKHHPKGTKQGNQKNSTKHCHQNETNILSTPEIKAKRSDVSAKDKAPAKDKPSKETKEKQQTGSAKSSSSSVPPLAPNRQHCAQVRTRRLMKELQDIRRLGDDFITVELADDNLYDWNVKLHQVDKDSALWQDMKETNTEYILLNISFPDNFPFSPPFVRVLTPRLENGYVLDGGAICMELLTPRGWSSAYTVEAVMRQFAASLVKGQGRICRKAGKSKKAFSRKEAEATFKSLVKTHEKYGWVSPPVSDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5U9A9</accession>
    <name>W5U9A9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL48</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L48, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Mrpl48</name>
        <name evidence="12" type="synonym">mrpl48</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the mitochondrion-specific ribosomal protein mL48 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT409079">
        <property type="protein sequence ID" value="AHH38553.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017344861.1">
        <property type="nucleotide sequence ID" value="XM_017489372.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U9A9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032437"/>
    <dbReference type="GeneID" id="108277062"/>
    <dbReference type="KEGG" id="ipu:108277062"/>
    <dbReference type="CTD" id="51642"/>
    <dbReference type="GeneTree" id="ENSGT00390000012955"/>
    <dbReference type="OMA" id="MKQHTEA"/>
    <dbReference type="OrthoDB" id="5984298at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005761">
        <property type="term" value="C:mitochondrial ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.600:FF:000006">
        <property type="entry name" value="39S ribosomal protein L48, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="entry name" value="Ribosomal protein S10 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027487">
        <property type="entry name" value="Ribosomal_mL48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_uS10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_uS10_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13473:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13473">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN L48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="Ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 10 12" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS10" evidence="9">
        <location>
            <begin position="88"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00071667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00084068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AHH38553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017344861.1"/>
        </source>
    </evidence>
    <sequence length="208" mass="23732" checksum="0DE8612579B6A096" modified="2014-04-16" version="1">MNALTGKVLLMQNTRALVQAVSLIRSPVLKQPVSIGAFMPDRQYRSMPTHGIGRYKYLLPKEMQKKKKEKAQMKEIKAATDTEYGVLNMTISGYDMTLVEHYAQYIHKLCNRLEIKVTDSYALPTKSTEVMVMPEQGTKMYVDVILKTHERVVQISRMKATLAPVLMEVLLKNQPEGVQLCIKEHTEAEYKARFKARPELEGLLAQMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5U9U5</accession>
    <name>W5U9U5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AP-1 complex subunit mu-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">AP1M2</name>
        <name evidence="13" type="synonym">ap1m2</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasmic vesicle</location>
            <location evidence="9">Clathrin-coated vesicle membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="JT408396">
        <property type="protein sequence ID" value="AHH38329.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017337795.1">
        <property type="nucleotide sequence ID" value="XM_017482306.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U9U5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000024468"/>
    <dbReference type="GeneID" id="108273201"/>
    <dbReference type="KEGG" id="ipu:108273201"/>
    <dbReference type="CTD" id="10053"/>
    <dbReference type="GeneTree" id="ENSGT00940000159089"/>
    <dbReference type="OMA" id="HSRLEIM"/>
    <dbReference type="OrthoDB" id="10259133at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030665">
        <property type="term" value="C:clathrin-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09259">
        <property type="entry name" value="AP-1_Mu1B_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14835">
        <property type="entry name" value="AP1_Mu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1170:FF:000002">
        <property type="entry name" value="AP-1 complex subunit mu-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000006">
        <property type="entry name" value="AP-1 complex subunit mu-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018240">
        <property type="entry name" value="Clathrin_mu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00314">
        <property type="entry name" value="CLATHRINADPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00990">
        <property type="entry name" value="CLAT_ADAPTOR_M_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7 9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="10">
        <location>
            <begin position="168"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AHH38329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017337795.1"/>
        </source>
    </evidence>
    <sequence length="423" mass="48141" checksum="511120D81B1BA5C6" modified="2014-04-16" version="1">MSASAVFILDLKGKVLICRNYKGDVDMSEIDHFLPLLLQQEEEGLMCPVISHGSVHFMWIKHSNLYLVATTNKNSNASLVYSFLYKLVEVFTEYFKELEEESIQDNFVVVYELLDELMDFGFPQTTDSKILQEYITQEGNKLEVTKAKVPTTVTNAVSWRSEGIKYKKNEVFIDVIESINVLVNANGSVMSSDIVGSIKLKTMLSGMPELRLGLNDRVLFALTGRDKGKTVAMEDVKFHQCVRLSRFENDRTISFIPPDGESELMSYRINTHVKPLIWIESVIEKFSHSRVEIMVKAKGQFKKQSVANNVEVRVPVPSDADSPKFKTSTGHAKYVPEKNLVVWTIKSFPGGKEFLMRAHFGLPSVEKDELEGKPPVTVKFEIPYFTVSGIQVRYMKIIEKSGYQALPWVRYITQSGDYQLRTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5U9U9</accession>
    <name>W5U9U9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3 5">F-box and leucine-rich protein 22</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">FBXL22</name>
        <name evidence="5" type="synonym">fbxl22</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT408408">
        <property type="protein sequence ID" value="AHH38334.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047015849.1">
        <property type="nucleotide sequence ID" value="XM_047159893.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U9U9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032247"/>
    <dbReference type="GeneID" id="108275137"/>
    <dbReference type="KEGG" id="ipu:108275137"/>
    <dbReference type="GeneTree" id="ENSGT00390000003748"/>
    <dbReference type="OMA" id="LQVCNIE"/>
    <dbReference type="OrthoDB" id="549243at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050648">
        <property type="entry name" value="F-box_LRR-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13382">
        <property type="entry name" value="MITOCHONDRIAL ATP SYNTHASE COUPLING FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AHH38334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_047015849.1"/>
        </source>
    </evidence>
    <sequence length="227" mass="25904" checksum="28476997708F93EC" modified="2014-04-16" version="1">MHLIDLNQECLLHLFSFLDKNSRRNLSLTCKRLRVAFLDPALWSLLNFFSPSELMRNNFILGPFLRYLNICWHSSRVKVCNIEDFKKTTFQRDLCSKHVSLVSHFLDRVCHMCPNLLSLTLSGCGHITDGDVINVLSSCRQLQRLCLENCCRVTDVVLQAVVTHGGSLQEVRVDFCRNVTHTGLQACREKRPGVRLFAERSAGMIPDTQPGEKLYITKALQKVLILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5UAV0</accession>
    <name>W5UAV0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cullin-5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CUL5</name>
        <name evidence="15" type="synonym">cul5a</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10 11">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="EMBL" id="JT410499">
        <property type="protein sequence ID" value="AHH39088.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017346294.1">
        <property type="nucleotide sequence ID" value="XM_017490805.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UAV0"/>
    <dbReference type="GeneID" id="108277836"/>
    <dbReference type="CTD" id="327215"/>
    <dbReference type="GeneTree" id="ENSGT00940000156648"/>
    <dbReference type="OMA" id="FWINQQF"/>
    <dbReference type="OrthoDB" id="27073at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031461">
        <property type="term" value="C:cullin-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000142">
        <property type="entry name" value="Cullin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000009">
        <property type="entry name" value="Cullin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000014">
        <property type="entry name" value="Cullin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000017">
        <property type="entry name" value="Cullin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.130:FF:000004">
        <property type="entry name" value="Cullin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016157">
        <property type="entry name" value="Cullin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01256">
        <property type="entry name" value="CULLIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cullin family profile" evidence="12">
        <location>
            <begin position="403"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AHH39088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017346294.1"/>
        </source>
    </evidence>
    <sequence length="780" mass="90982" checksum="EB03A0C7BD08FB25" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5UB72</accession>
    <name>W5UB72_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphatidylinositol-glycan biosynthesis class W protein</fullName>
            <ecNumber evidence="5">2.3.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PIGW</name>
        <name evidence="8" type="synonym">pigw</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">A acetyltransferase, which acetylates the inositol ring of phosphatidylinositol during biosynthesis of GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PIGW family.</text>
    </comment>
    <dbReference type="EC" id="2.3.-.-" evidence="5"/>
    <dbReference type="EMBL" id="JT410843">
        <property type="protein sequence ID" value="AHH39208.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017314872.1">
        <property type="nucleotide sequence ID" value="XM_017459383.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UB72"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018629"/>
    <dbReference type="GeneID" id="108259696"/>
    <dbReference type="KEGG" id="ipu:108259696"/>
    <dbReference type="CTD" id="284098"/>
    <dbReference type="OMA" id="GLYVMQP"/>
    <dbReference type="OrthoDB" id="15270at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032216">
        <property type="term" value="F:glucosaminyl-phosphatidylinositol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009447">
        <property type="entry name" value="PIGW/GWT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20661">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20661:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06423">
        <property type="entry name" value="GWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017321">
        <property type="entry name" value="GWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="243"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="306"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="374"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="442"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="466"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU280819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AHH39208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017314872.1"/>
        </source>
    </evidence>
    <sequence length="497" mass="55302" checksum="1C614CFC7B7E7F00" modified="2014-04-16" version="1">METSKAMAETEIKVAFVSNLNGTTLTEVSLGSFLAPLCLLSRGLFVLVFHLNKGVLPLRSSAHLLLDFILLITPLVLSCTVLSDVLHWVIILLAVVDVCAVFILYSKRHKKPAGHQSMIDSFGHSHVESSLVPFLTAFRVLVNVKTAISILAVDFTVFPRRYAKTEMYGTGVMDFGVGAYVMANAMVCPEARGKKIQGSKLSHLAKQVFSIWPLVVLGFARLASVKATGYHEHITEYGLHWNFFFTLAIVRVVASALLAVFPINVSWLLSLLIIGAYQVVLEKTDLKSFLTQNAERSSFMQANKEGIFSVVGYVAVYMAGVQVGVYLMQTRTLVKDWIRVIRNLLLGSFGLWVALHACQMHIEPVSRRMANLPFCIWTIAQSLFFLSCLGIADIALLFAKVVSSFTAVSASWNPRHRSETERKIMNKLDALCLIQAVNRNQLLFFLLANVMTGLTNILVDTLKSNDLFAVCVLLSYMFINSLIIFILHVNNITVKFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5UD22</accession>
    <name>W5UD22_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="23 25">Chromodomain-helicase-DNA-binding protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">CHD4</name>
        <name evidence="25" type="synonym">chd4a</name>
    </gene>
    <organism evidence="23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="23">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">SDA103</strain>
        </source>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="JT412214">
        <property type="protein sequence ID" value="AHH39755.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017337579.2">
        <property type="nucleotide sequence ID" value="XM_017482090.3"/>
    </dbReference>
    <dbReference type="GeneID" id="108273091"/>
    <dbReference type="KEGG" id="ipu:108273091"/>
    <dbReference type="CTD" id="558344"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016581">
        <property type="term" value="C:NuRD complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18667">
        <property type="entry name" value="CD1_tandem_CHD3-4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18662">
        <property type="entry name" value="CD2_tandem_CHD3-4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18056">
        <property type="entry name" value="DEXHc_CHD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15531">
        <property type="entry name" value="PHD1_CHD_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15532">
        <property type="entry name" value="PHD2_CHD_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000037">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000003">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000001">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000001">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000017">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 3 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000011">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000015">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012957">
        <property type="entry name" value="CHD_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009462">
        <property type="entry name" value="CHD_II_SANT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012958">
        <property type="entry name" value="CHD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009463">
        <property type="entry name" value="DUF1087"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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            <dbReference type="RefSeq" id="XP_017337579.2"/>
        </source>
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    <sequence length="1946" mass="221044" checksum="6F7142D3508FB806" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="9">Fat storage-inducing transmembrane protein 2</fullName>
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        <name evidence="9 11" type="primary">fitm2</name>
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        <name type="scientific">Ictalurus punctatus</name>
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        <name type="synonym">Silurus punctatus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
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        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <tissue evidence="9">Mixed</tissue>
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    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10">SDA103</strain>
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    <reference evidence="11" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="FITM1/2"/>
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        <property type="entry name" value="ACYL-COENZYME A DIPHOSPHATASE FITM2"/>
        <property type="match status" value="1"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
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    <feature type="transmembrane region" description="Helical" evidence="8">
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    <feature type="transmembrane region" description="Helical" evidence="8">
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AHH38582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017342714.1"/>
        </source>
    </evidence>
    <sequence length="257" mass="29576" checksum="72F413D62E5D662B" modified="2014-04-16" version="1">MSALSGVVKNSLPFLVFNVTIGRKYLPWFFFALSFGGSLLKELSVVPSSYFSSSGNVLNVYFVKVSWGWNLVLLLPFILLSNSYDRSFIFVCKRLTCLVVATAIWYSCTETFFYIEDVTGVCYKSDKMAEILEGFNTKAECRNAGHFWDGFDISGHSFILSYSTLLIVEEMVPMLYLVQHYQNRTTVLDALYLALNAIAIIWVWMFACTSVYFHNTFQKFLGTLFGVLGWYMTYKVWYMNPFSPGLPPRQIDRKQHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>W5UGZ6</accession>
    <name>W5UGZ6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 16" type="primary">rab18b</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Golgi apparatus</location>
            <location evidence="12">cis-Golgi network membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="EMBL" id="JT410154">
        <property type="protein sequence ID" value="AHH38947.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017351018.1">
        <property type="nucleotide sequence ID" value="XM_017495529.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UGZ6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000016038"/>
    <dbReference type="GeneID" id="108280497"/>
    <dbReference type="KEGG" id="ipu:108280497"/>
    <dbReference type="CTD" id="445055"/>
    <dbReference type="GeneTree" id="ENSGT00940000157325"/>
    <dbReference type="OMA" id="HECIKIL"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01863">
        <property type="entry name" value="Rab18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000430">
        <property type="entry name" value="Probable Ras-related protein Rab-18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AHH38947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017351018.1"/>
        </source>
    </evidence>
    <sequence length="205" mass="23040" checksum="599D67AA909CC8F8" modified="2014-04-16" version="1">MDDDVLTTLKILIIGESGVGKSSLLLRFTDDTFDPELAATIGVDFKVKTITVDGNRAKLAIWDTAGQERFRTLTPSYYRGAQGVILVYDVTKRDSFTKLENWLSELETYCTRNDLVKMLIGNKIDKDNREMDRNEGLKFARKHSMLFIEASAKTRDGVQCAFEELVEKILQTPGLWESSVQNHSVQLGEQQAHSYSACGGYCSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>W5UJY7</accession>
    <name>W5UJY7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mothers against decapentaplegic homolog</fullName>
            <shortName evidence="9">MAD homolog</shortName>
            <shortName evidence="9">Mothers against DPP homolog</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">SMAD family member</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SMAD3</name>
        <name evidence="15" type="synonym">smad3a</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the dwarfin/SMAD family.</text>
    </comment>
    <dbReference type="EMBL" id="JT416292">
        <property type="protein sequence ID" value="AHH42000.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017314642.1">
        <property type="nucleotide sequence ID" value="XM_017459153.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UJY7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000028880"/>
    <dbReference type="GeneID" id="108259569"/>
    <dbReference type="KEGG" id="ipu:108259569"/>
    <dbReference type="CTD" id="58092"/>
    <dbReference type="GeneTree" id="ENSGT00940000153499"/>
    <dbReference type="OMA" id="VAYHEPP"/>
    <dbReference type="OrthoDB" id="5794312at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071144">
        <property type="term" value="C:heteromeric SMAD protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070411">
        <property type="term" value="F:I-SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032924">
        <property type="term" value="P:activin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009953">
        <property type="term" value="P:dorsal/ventral pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009880">
        <property type="term" value="P:embryonic pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060395">
        <property type="term" value="P:SMAD protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10491">
        <property type="entry name" value="MH1_SMAD_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10985">
        <property type="entry name" value="MH2_SMAD_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.10:FF:000001">
        <property type="entry name" value="Mothers against decapentaplegic homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.520.10:FF:000001">
        <property type="entry name" value="Mothers against decapentaplegic homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.520.10">
        <property type="entry name" value="SMAD MH1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013790">
        <property type="entry name" value="Dwarfin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003619">
        <property type="entry name" value="MAD_homology1_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013019">
        <property type="entry name" value="MAD_homology_MH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017855">
        <property type="entry name" value="SMAD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001132">
        <property type="entry name" value="SMAD_dom_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036578">
        <property type="entry name" value="SMAD_MH1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703:SF53">
        <property type="entry name" value="MOTHERS AGAINST DECAPENTAPLEGIC HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703">
        <property type="entry name" value="SMAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03165">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03166">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00523">
        <property type="entry name" value="DWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00524">
        <property type="entry name" value="DWB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56366">
        <property type="entry name" value="SMAD MH1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51075">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51076">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MH1" evidence="11">
        <location>
            <begin position="9"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="MH2" evidence="12">
        <location>
            <begin position="232"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="167"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="192"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AHH42000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017314642.1"/>
        </source>
    </evidence>
    <sequence length="425" mass="48017" checksum="E0B9560831E1C4AC" modified="2014-04-16" version="1">MSILPFTPPIVKRLLGWKKGEQNSQEEKWCEKAVKSLVKKLKKTGQLDELEKAITTQNGNTKCITIPRSLDGRLQVSHRKGLPHVIYCRLWRWPDLQSHHELRAMELCEFAFHMKKDEVCVNPYHYQRVETPVLPPVLVPRHTEIPSEFPPLDDYSHSIPENTNFPAGIEPPSNYIAETPPPGYLSEDGETSDYSLNPSMDTGSPSLSPNSLSPPPSNLDLQPVTYCEPAFWCSISYYELNQRVGEIFHASQPSLTVDGFTDPSNSERFCLGLLSNINRNAAVELTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSIRCSSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5UKW6</accession>
    <name>W5UKW6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Alpha-galactosidase</fullName>
            <ecNumber evidence="10">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">NAGA</name>
        <name evidence="15" type="synonym">naga</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3 10">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 27 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="10"/>
    <dbReference type="EMBL" id="JT417312">
        <property type="protein sequence ID" value="AHH42335.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017350165.1">
        <property type="nucleotide sequence ID" value="XM_017494676.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UKW6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000025626"/>
    <dbReference type="GeneID" id="108279976"/>
    <dbReference type="KEGG" id="ipu:108279976"/>
    <dbReference type="CTD" id="4668"/>
    <dbReference type="GeneTree" id="ENSGT00390000008751"/>
    <dbReference type="OrthoDB" id="5795902at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004557">
        <property type="term" value="F:alpha-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019377">
        <property type="term" value="P:glycolipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016139">
        <property type="term" value="P:glycoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14792">
        <property type="entry name" value="GH27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000070">
        <property type="entry name" value="Alpha-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002241">
        <property type="entry name" value="Glyco_hydro_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000111">
        <property type="entry name" value="Glyco_hydro_27/36_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035373">
        <property type="entry name" value="Melibiase/NAGA_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452:SF83">
        <property type="entry name" value="ALPHA-GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452">
        <property type="entry name" value="ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16499">
        <property type="entry name" value="Melibiase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17450">
        <property type="entry name" value="Melibiase_2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00740">
        <property type="entry name" value="GLHYDRLASE27"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00512">
        <property type="entry name" value="ALPHA_GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0458">Lysosome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540433" description="Alpha-galactosidase" evidence="11">
        <location>
            <begin position="19"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha galactosidase A C-terminal" evidence="12">
        <location>
            <begin position="313"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AHH42335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017350165.1"/>
        </source>
    </evidence>
    <sequence length="425" mass="47943" checksum="8CC1C9AB981D7E38" modified="2014-04-16" version="1">MQQTVVIFLLAFSSATQALDNGLMRTPPMGWLAWERFRCDIDCENDPQNCISEVLFRDMADRLAEDGWRELGYVYVNIDDCWASKSRDTQGRLQADPKRFPGGIAKLARYVHDRGLKLGIYGDLGTYTCGGYPGTTLDKIETDAQTFAEWEVDMLKLDGCYSNATDQEQGYPRMSKALNATGRPIGYSCSWPAYQGGLPPKINYTLLGEICNLWRNYGDIEDSWDSILNIVDWFAKNQDILQPAAGPGRWNDPDMLVIGDFGLSMDQSRSQMALWAIMAAPLFMSNDLRTLNSGARAILQNKAAIAINQDPLGIQGRCLIKESNGIEVFWRPLSNKASALVFFSRRTDMPYRYQTSLKVLDYTPGSYKVYDVFMEKTMPTLKDTTEFVVSINPSGVVMWYVVPSAEWQEQPKFHQAIVKADSFIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>W5UKY0</accession>
    <accession>A0A2D0RSM8</accession>
    <name>W5UKY0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3 5">Protein FAM32A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fam32al</name>
        <name evidence="5" type="synonym">fam32a</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2023-07" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM32 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT418739">
        <property type="protein sequence ID" value="AHH42829.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017333509.2">
        <property type="nucleotide sequence ID" value="XM_017478020.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UKY0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000037125"/>
    <dbReference type="GeneID" id="108270957"/>
    <dbReference type="KEGG" id="ipu:108270957"/>
    <dbReference type="CTD" id="26017"/>
    <dbReference type="GeneTree" id="ENSGT00390000013811"/>
    <dbReference type="OrthoDB" id="205403at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013865">
        <property type="entry name" value="FAM32A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13282">
        <property type="entry name" value="PROTEIN FAM32A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13282:SF6">
        <property type="entry name" value="PROTEIN FAM32A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08555">
        <property type="entry name" value="FAM32A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="21"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AHH42829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017333509.2"/>
        </source>
    </evidence>
    <sequence length="112" mass="13042" checksum="55761B376B359D96" modified="2014-04-16" version="1">MAEYAAVQKGSLKLKGIGDISAGKKKKKKDKETKRLEKQVLTTSTEEETTKKAYVDKRTPAQIAFDKIQEKRQIERILNKASKTHKRRVEEFNRHLDTLTEHYDIPKVSWTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5UQJ0</accession>
    <name>W5UQJ0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Gastrin/cholecystokinin type B receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Cholecystokinin-2 receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">CCKAR</name>
        <name evidence="22" type="synonym">cckar</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Receptor for gastrin and cholecystokinin. The CCK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT412672">
        <property type="protein sequence ID" value="AHH39948.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JT415852">
        <property type="protein sequence ID" value="AHH41838.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017327864.1">
        <property type="nucleotide sequence ID" value="XM_017472375.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UQJ0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003109"/>
    <dbReference type="GeneID" id="108267885"/>
    <dbReference type="KEGG" id="ipu:108267885"/>
    <dbReference type="CTD" id="886"/>
    <dbReference type="OMA" id="NIAPPCE"/>
    <dbReference type="OrthoDB" id="5987936at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015054">
        <property type="term" value="F:gastrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008188">
        <property type="term" value="F:neuropeptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009126">
        <property type="entry name" value="Cholcskin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000314">
        <property type="entry name" value="Gastrin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24238:SF81">
        <property type="entry name" value="CHOLECYSTOKININ RECEPTOR TYPE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24238">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01822">
        <property type="entry name" value="CCYSTOKININR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00527">
        <property type="entry name" value="GASTRINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 16" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10 16 20" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 16" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 16 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="325"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="19">
        <location>
            <begin position="68"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="281"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="AHH41838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017327864.1"/>
        </source>
    </evidence>
    <sequence length="461" mass="50808" checksum="83ACDED6F6D0E136" modified="2014-04-16" version="1">METFTIHDMLINSTDIYKILCEFAFRNISECENASDPGTHALTPEPKDINQTVRIILYCVIFLLSVLGNSLIIAVLVRNRRMRTVTNLFLLSLAVSDLMLCMFCMPFTLIPNLMKNFVFGSGICKVATYFMGISVSVSTFNLVAISLERYSAICNPLTSRTWQTKSHAAKVISATWLLSFLLMVPYPISSTLVPYRRTNNSTGNMCRHIWPSDIIQQSWYVFLLLILILVPGIVMMTAYGLISVELYRGIKFEMANRKASQERQSSTGSLKPSECDGCYLQPSKKKRSDASTNQQSTLLASSSKPKLNRVYSNSPTANLMAKKRVIRMLLVIVVLFFLCWTPVFAANAWRAFDKRSADRLLSGAPISFIHLLSYTSACVNPIIYCFMNKRFRQGMLATFSCCTGHVDREGRGSGRLSAGGGGGGGALGTLGCAGAGGKENTQSSGTLTRLTYSSVRGSAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0B5H384</accession>
    <name>A0A0B5H384_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Claudin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">CLDN33c</name>
        <name evidence="11" type="synonym">cldn26</name>
        <name evidence="11" type="synonym">si:dkey-98f17.3</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11" key="1">
        <citation type="journal article" date="2015" name="Comp. Biochem. Physiol. Part D Genomics Proteomics" volume="13" first="60" last="69">
            <title>Claudin multigene family in channel catfish and their expression profiles in response to bacterial infection and hypoxia as revealed by meta-analysis of RNA-Seq datasets.</title>
            <authorList>
                <person name="Sun L."/>
                <person name="Liu S."/>
                <person name="Bao L."/>
                <person name="Li Y."/>
                <person name="Feng J."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="25681604"/>
            <dbReference type="DOI" id="10.1016/j.cbd.2015.01.002"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell junction</location>
            <location evidence="8">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="EMBL" id="KM870793">
        <property type="protein sequence ID" value="AJF34907.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001316176.1">
        <property type="nucleotide sequence ID" value="NM_001329247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0B5H384"/>
    <dbReference type="GeneID" id="108255719"/>
    <dbReference type="KEGG" id="ipu:108255719"/>
    <dbReference type="CTD" id="793143"/>
    <dbReference type="GeneTree" id="ENSGT00940000161922"/>
    <dbReference type="OrthoDB" id="8612291at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0796">Tight junction</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="128"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU060637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AJF34907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="NP_001316176.1"/>
        </source>
    </evidence>
    <sequence length="231" mass="26013" checksum="F7BC271B36652C71" modified="2015-04-01" version="1">MYRERKEPELTMVLLTTKIVQRASLFVAFGGLVTTFITTFLPLWKTMNSELNEMENWYEGLWHMCIFTEEVGLHCKAFESLLALPPVTLASRILMCVSIATGFLGVLAAFFGLDGVEIGAGRDRLKRGLLILGGVLIWVSGLTTLAPVSLIAYVMVVEFWDGGLPDVMPRWEYGEAMFSAWFSGLLLVIGGSFIFVAVCMRDHEEKQQREIFSPAHELQPRTQHYLKTEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PKJ0</accession>
    <name>A0A2D0PKJ0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Kelch repeat and BTB domain-containing protein 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">kbtbd8</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017306542.2">
        <property type="nucleotide sequence ID" value="XM_017451053.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PKJ0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033671"/>
    <dbReference type="GeneID" id="108255237"/>
    <dbReference type="KEGG" id="ipu:108255237"/>
    <dbReference type="CTD" id="84541"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048741">
        <property type="term" value="P:skeletal muscle fiber development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000006">
        <property type="entry name" value="Kelch repeat and BTB domain-containing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF433">
        <property type="entry name" value="KELCH REPEAT AND BTB DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="73"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017306542.2"/>
        </source>
    </evidence>
    <sequence length="626" mass="70932" checksum="309CF7888B11A602" modified="2023-02-22" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PPL4</accession>
    <name>A0A2D0PPL4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein si:dkey-98f17.5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">si:dkey-98f17.5</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017307371.1">
        <property type="nucleotide sequence ID" value="XM_017451882.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PPL4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029558"/>
    <dbReference type="GeneID" id="108255717"/>
    <dbReference type="KEGG" id="ipu:108255717"/>
    <dbReference type="OrthoDB" id="1716625at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027094">
        <property type="entry name" value="Mitofusin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10465:SF4">
        <property type="entry name" value="DYNAMIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10465">
        <property type="entry name" value="TRANSMEMBRANE GTPASE FZO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="381"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Dynamin N-terminal" evidence="7">
        <location>
            <begin position="60"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017307371.1"/>
        </source>
    </evidence>
    <sequence length="450" mass="50956" checksum="6701B4D7DEC316BD" modified="2017-12-20" version="1">MSGSKKPRSVANTLETAITTPSERILQECHTLYIDTENGLVKIASRLGLRLLPPRKKIIVMIMGNHSAGKSSFINWYVEEHIQKTGVAIETQGFTFITSGRKRESLTGNATLHLYPHFRPLLEFKGLMDYLSAEISTSKQKKFSLVTFVDTPGLVDGDMVYPFDVNAAITSFGEQADLIFVFFDPMGQALCKRTLNIVEKLSEKSGDKLRFYLSKADEAGRETDRQRVMMQIVQELCRRPGLNKCGFEMPTIYIPDPLRLSRCVNQIDEVCETIEKSINQAVQKTLDQLEKDCDLVASSISNKLEQDRRNVSNNKNSRFNSFLCGVLAIFLPAVFVISFLINTFVPEELGALVGDGLAVTLYRTSGIVLYLWEWIPEDSQVVAVIIFGATCYFFLFLARYFARQSSKTLTKKEKKNLMECSDYVKDVLKAKKKKLYEEYLKQCAAECDFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PTF0</accession>
    <name>A0A2D0PTF0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Tubulointerstitial nephritis antigen-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tinagl1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017309833.1">
        <property type="nucleotide sequence ID" value="XM_017454344.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PTF0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000031930"/>
    <dbReference type="GeneID" id="108256990"/>
    <dbReference type="KEGG" id="ipu:108256990"/>
    <dbReference type="CTD" id="64129"/>
    <dbReference type="OrthoDB" id="190265at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030414">
        <property type="term" value="F:peptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02620">
        <property type="entry name" value="Peptidase_C1A_CathepsinB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000037">
        <property type="entry name" value="Tubulointerstitial nephritis antigen-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008037">
        <property type="entry name" value="Pacifastin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036201">
        <property type="entry name" value="Pacifastin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001212">
        <property type="entry name" value="Somatomedin_B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05375">
        <property type="entry name" value="Pacifastin_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57283">
        <property type="entry name" value="PMP inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00524">
        <property type="entry name" value="SMB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50958">
        <property type="entry name" value="SMB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018740178" evidence="6">
        <location>
            <begin position="22"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="SMB" evidence="7">
        <location>
            <begin position="54"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017309833.1"/>
        </source>
    </evidence>
    <sequence length="477" mass="54544" checksum="71AC849C4A8541B9" modified="2017-12-20" version="1">MMMRMRLVVVISAVLLLMVDSSVMERILSRSRRELASPLHARGIRDPFGSYCERRGGCCEGRNDECTMPYLDTICYCDLFCNRTVSDCCPDFWGHCLGIQPPPRGTCERNGHRIHSGATYKENCNLCTCSATGQWVCEQYACLIEQDMIQAVNRGSYGWKAANYSQFWGMSLDEGLRYRLGTQRPSRIIMSMNEIQMNMDNNEHLPSYFNAAEKWPGKIHEPLDQGNCAASWAFSTAAVASDRISIQSMGTMTPQLSPQNLISCDTRNQGGCAGGRIDGAWWYLRRRGVVTEECYPFNPPQQTPAEVSRCMMQSRSVGRGKRQATARCPNTHNYNNEIYQSTPPYRLSSNEKEIMKEIMDNGPVQAILEVHEDFFVYKSGIYRHIDVNVHKAAQYRKHGTHSVRIIGWGEERDYNGETHKYWIAANSWGKNWGEDGYFRIARGENECQIEAFVIGAWGRITMEDMYSDHHHHHGRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PU17</accession>
    <name>A0A2D0PU17_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ddx5</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="RefSeq" id="XP_017310033.1">
        <property type="nucleotide sequence ID" value="XM_017454544.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PU17"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000011502"/>
    <dbReference type="GeneID" id="108257112"/>
    <dbReference type="KEGG" id="ipu:108257112"/>
    <dbReference type="CTD" id="1655"/>
    <dbReference type="OMA" id="STMPKFE"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001556">
        <property type="term" value="P:oocyte maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="13">
        <location>
            <begin position="100"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="131"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="334"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="478"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="8">
        <location>
            <begin position="100"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="495"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="518"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="542"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="564"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="601"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017310033.1"/>
        </source>
    </evidence>
    <sequence length="625" mass="70440" checksum="A3E844A020E48039" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PUZ2</accession>
    <name>A0A2D0PUZ2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation factor 1-alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">eef1a1l3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Translation elongation factor that catalyzes the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis. Base pairing between the mRNA codon and the aa-tRNA anticodon promotes GTP hydrolysis, releasing the aa-tRNA from EEF1A1 and allowing its accommodation into the ribosome. The growing protein chain is subsequently transferred from the P-site peptidyl tRNA to the A-site aa-tRNA, extending it by one amino acid through ribosome-catalyzed peptide bond formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017308925.1">
        <property type="nucleotide sequence ID" value="XM_017453436.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PUZ2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005854"/>
    <dbReference type="GeneID" id="108256503"/>
    <dbReference type="KEGG" id="ipu:108256503"/>
    <dbReference type="CTD" id="407641"/>
    <dbReference type="OMA" id="AHVTCRF"/>
    <dbReference type="OrthoDB" id="342024at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
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        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PRK12317.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
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    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5 10" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="7 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="5"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017308925.1"/>
        </source>
    </evidence>
    <sequence length="458" mass="50220" checksum="EFBA40FEE62925C1" modified="2017-12-20" version="1">MGREKIHINLVIIGHVDSGKSTTTGHLVYKCGGVDPRTLDKYEKAAAQMGKGSFKYAWVLDKLKAERERGITIDISLLKFNTQKYVFTIIDAPGHRDFIKNMITGTSQADAALLVVSAAKGEFEAGVSRNGQTREHVLLAYTLGVKQLVVCVNKMDLTEPPFSQKRYEEVVKNVTVFIKKIGYDPTAVPFVPLSGWCGDNMLAPSQKMSWFKGWKIKRKDGFSSGKTLLEVLDSLYPPVRTASKPLRLPLQDVYKIGGVGTVPVGKIETGILKPGMVLTFSPAKLTAEVKSIEMHHQGLQTALPGHNVGFNIKNVAVKNLRRGDVAGNAQQDPPSDVNSFIAQVIILNHPGKIKSGYSPVLDCHTTHVTCSFAELQEKLDRRTGKKLEDIPPFLVSGDAATVKFVPIKPLCVESFFSYPPLGRFAARDLKQTVAVGVIKSVEKVDQARRTAQKAQALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PXN1</accession>
    <name>A0A2D0PXN1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Antigen WC1.1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC108257489</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017310787.1">
        <property type="nucleotide sequence ID" value="XM_017455298.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PXN1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000002742"/>
    <dbReference type="GeneID" id="108257489"/>
    <dbReference type="KEGG" id="ipu:108257489"/>
    <dbReference type="OMA" id="SEQICQD"/>
    <dbReference type="OrthoDB" id="536948at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031638">
        <property type="term" value="P:zymogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000016">
        <property type="entry name" value="Scavenger receptor cysteine-rich protein type 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000002">
        <property type="entry name" value="Scavenger receptor cysteine-rich type 1 protein M130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF15">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043736127" evidence="13">
        <location>
            <begin position="19"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="443"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="604"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="654"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="604"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="692"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017310787.1"/>
        </source>
    </evidence>
    <sequence length="714" mass="78302" checksum="2D7F3E4834D42329" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PZ01</accession>
    <name>A0A2D0PZ01_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transcription factor AP-2-beta isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tfap2b</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AP-2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017311257.1">
        <property type="nucleotide sequence ID" value="XM_017455768.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PZ01"/>
    <dbReference type="GeneID" id="108257756"/>
    <dbReference type="KEGG" id="ipu:108257756"/>
    <dbReference type="CTD" id="7021"/>
    <dbReference type="OrthoDB" id="6252992at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004979">
        <property type="entry name" value="TF_AP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008122">
        <property type="entry name" value="TF_AP2_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013854">
        <property type="entry name" value="TF_AP2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10812">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10812:SF14">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-2-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03299">
        <property type="entry name" value="TF_AP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01750">
        <property type="entry name" value="AP2BTNSCPFCT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01748">
        <property type="entry name" value="AP2TNSCPFCT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor AP-2 C-terminal" evidence="8">
        <location>
            <begin position="222"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="17"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="428"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="440"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017311257.1"/>
        </source>
    </evidence>
    <sequence length="450" mass="49359" checksum="25F38764B97D033E" modified="2017-12-20" version="1">MLWKLVENVKYEDIYEDRHDGVPGHSSRLSQLGSVSQAPYSSAPPLSHAPSSDFQPPYFPPPYQPLPYPQSQDPYSHVNDPYALNALHQQHQQHPWGSRQRQDVGGESASLLPQPRASLPPQLSGLDPRTRDYSSSVRRPDVLLHSAPHGLEPGMGDSLSLHGLAHGMDDTHAVEDANHSGMNILDQSVIKKVPMPHKSVTSLMMSKDGLIGGVTVNVNEVFCSVPGRLSLLSSTSKYKVTVGEVQRRLSPPECLNASLLGGVLRRAKSKNGGRSLREKLEKIGLNLPAGRRKAANVTLLTSLVEGEAVHLARDFGYICETEFPTKAVSEYLNRQHTDPNEIHARKNMLLATKQLCKEFTDLLAQDRTPLGNSRPTPILEPGIQSCLSHFSFITHGFGSPAICAALTALQNYLTEALKGLDKMFINNQTSNRHTPADGSKGGEKDEKHRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PZQ5</accession>
    <name>A0A2D0PZQ5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ena/VASP-like protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Ena/vasodilator-stimulated phosphoprotein-like</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">evlb</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. EVL enhances actin nucleation and polymerization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Stress fiber</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Ena/VASP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017311973.1">
        <property type="nucleotide sequence ID" value="XM_017456484.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PZQ5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029780"/>
    <dbReference type="GeneID" id="108258102"/>
    <dbReference type="KEGG" id="ipu:108258102"/>
    <dbReference type="CTD" id="402929"/>
    <dbReference type="OMA" id="RTHFGIN"/>
    <dbReference type="OrthoDB" id="31170at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005522">
        <property type="term" value="F:profilin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008154">
        <property type="term" value="P:actin polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030838">
        <property type="term" value="P:positive regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051289">
        <property type="term" value="P:protein homotetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01207">
        <property type="entry name" value="EVH1_Ena_VASP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22185">
        <property type="entry name" value="WH2_hVASP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000004">
        <property type="entry name" value="Enah/Vasp-like, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000071">
        <property type="entry name" value="Enah/Vasp-like, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017354">
        <property type="entry name" value="VASP/EVL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038023">
        <property type="entry name" value="VASP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014885">
        <property type="entry name" value="VASP_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000697">
        <property type="entry name" value="WH1/EVH1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202:SF4">
        <property type="entry name" value="ENA_VASP-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202">
        <property type="entry name" value="SPROUTY-RELATED, EVH1 DOMAIN-CONTAINING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08776">
        <property type="entry name" value="VASP_tetra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00568">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038010">
        <property type="entry name" value="Vasodilator_Phospo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00461">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118370">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein, VASP, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50229">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0729">SH3-binding</keyword>
    <feature type="domain" description="WH1" evidence="13">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="136"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="194"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="253"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="194"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="305"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="320"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017311973.1"/>
        </source>
    </evidence>
    <sequence length="390" mass="42352" checksum="CE9309263F52A315" modified="2017-12-20" version="1">MSEQSICQARASVMVYDDTSKKWVPIKPGQQGFSRINIYHNTANNTFRVVGVKLQDQQVVINYSIVKGLKYNQATPTFHQWRDARQVYGLNFASKEEATTFSNAMLFALNVLSTQDGGPAVQRHVQNGPTAEELEVQRRQMEIQQQQQQQMQAHIERERRSSNSGSPFQGHPAVLSVAPPSIVPPVMSIGGPCPPPPPPPAPPGLPPPTAGAPPPPPPPLPLGGGGYGGHHHHHEDAHAPTGLAAMIAGAKLRRVQRSDESSGAKNDANRTSGGSGGLMEEMNALLARRRKAASQSEKDGDCQNDDANSSPMSRGQNADGAKKPWDRANSAERSSLVSRVRPVGSSTDTDALDLDRMKQEILEEVVRELHKVKDEIIDAIRHELSRISTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q2L8</accession>
    <name>A0A2D0Q2L8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Aftiphilin a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">aftpha</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017312532.1">
        <property type="nucleotide sequence ID" value="XM_017457043.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q2L8"/>
    <dbReference type="GeneID" id="108258432"/>
    <dbReference type="KEGG" id="ipu:108258432"/>
    <dbReference type="CTD" id="556043"/>
    <dbReference type="OrthoDB" id="5917212at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046359">
        <property type="entry name" value="Aftin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029205">
        <property type="entry name" value="Clathrin-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16156">
        <property type="entry name" value="AFTIPHILIN A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16156:SF10">
        <property type="entry name" value="AFTIPHILIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15045">
        <property type="entry name" value="Clathrin_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aftiphilin clathrin-binding box" evidence="2">
        <location>
            <begin position="596"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="507"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="401"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017312532.1"/>
        </source>
    </evidence>
    <sequence length="840" mass="88886" checksum="CE13FAB40C54F37C" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q5W8</accession>
    <name>A0A2D0Q5W8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Polyprenol dehydrogenase</fullName>
            <ecNumber evidence="17">1.1.1.441</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Dolichal reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">dhrsx</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Oxidoreductase that plays a key role in early steps of protein N-linked glycosylation by mediating two non-consecutive steps in dolichol biosynthesis. Acts both as a NAD(+)-dependent dehydrogenase and as a NADPH-dependent reductase during the conversion of polyprenol into dolichol. First catalyzes the NAD(+)-dependent dehydrogenation of polyprenol into polyprenal; polyprenal is then reduced into dolichal by SRD5A3. It then catalyzes the NADPH-dependent reduction of dolichal into dolichol. May also acts as a positive regulator of starvation-induced autophagy.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a di-trans,poly-cis-dolichol + NAD(+) = a di-trans,poly-cis-dolichal + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:80735"/>
            <dbReference type="Rhea" id="RHEA-COMP:19495"/>
            <dbReference type="Rhea" id="RHEA-COMP:19537"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16091"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:231637"/>
            <dbReference type="EC" id="1.1.1.441"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:80737"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a di-trans,poly-cis-dolichol + NADP(+) = a di-trans,poly-cis-dolichal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:80731"/>
            <dbReference type="Rhea" id="RHEA-COMP:19495"/>
            <dbReference type="Rhea" id="RHEA-COMP:19537"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16091"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:231637"/>
            <dbReference type="EC" id="1.1.1.441"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:80733"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a di-trans,poly-cis-polyprenol + NAD(+) = a di-trans,poly-cis-polyprenal + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:80719"/>
            <dbReference type="Rhea" id="RHEA-COMP:19496"/>
            <dbReference type="Rhea" id="RHEA-COMP:19536"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:67132"/>
            <dbReference type="ChEBI" id="CHEBI:231623"/>
            <dbReference type="EC" id="1.1.1.441"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:80720"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a di-trans,poly-cis-polyprenol + NADP(+) = a di-trans,poly-cis-polyprenal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:80723"/>
            <dbReference type="Rhea" id="RHEA-COMP:19496"/>
            <dbReference type="Rhea" id="RHEA-COMP:19536"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:67132"/>
            <dbReference type="ChEBI" id="CHEBI:231623"/>
            <dbReference type="EC" id="1.1.1.441"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:80724"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.441" evidence="17"/>
    <dbReference type="RefSeq" id="XP_017313692.3">
        <property type="nucleotide sequence ID" value="XM_017458203.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q5W8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034858"/>
    <dbReference type="GeneID" id="108259064"/>
    <dbReference type="KEGG" id="ipu:108259064"/>
    <dbReference type="CTD" id="207063"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05327">
        <property type="entry name" value="retinol-DH_like_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000490">
        <property type="entry name" value="Dehydrogenase/reductase SDR family member on chromosome X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF31">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00074632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00082366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00093201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00093233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00093592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017313692.3"/>
        </source>
    </evidence>
    <sequence length="326" mass="35912" checksum="E7F31F93A2BA1DEA" modified="2023-06-28" version="3">MWRLFAFILPVIRLYFIGVKVLIYQLLHKSFTLPVLPRQNGRVAIVTGGARGMGYETSRHLASLGTHVVIAGNEEEEGLVAVKKIQKQTGHRKVEFMYLDLASLKSVQQFVQRFKARGLPLHTLVNNAGVMLVPERKTEDGFELHFGLNYLGHFLLTNLLLDVLRDSGQSGGCSRIVTVSSATHYGGRLDLNDLQGRSCYCSHGAYSQSKLALVLFTYQLQERLWARADPVTANAVDPGMVDTDLYQNLCSPAQLAKKPVAKLLFRTPAEGASTAVYAAAASELEGLGGLYLYNGQKKISSASSYDKQLQEELWTKSCAMVGLQKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q6D7</accession>
    <name>A0A2D0Q6D7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC108259354 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108259354</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017314263.2">
        <property type="nucleotide sequence ID" value="XM_017458774.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q6D7"/>
    <dbReference type="GeneID" id="108259354"/>
    <dbReference type="KEGG" id="ipu:108259354"/>
    <dbReference type="OrthoDB" id="8446971at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34488">
        <property type="entry name" value="SI:CH211-245H14.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34488:SF1">
        <property type="entry name" value="SI:CH211-245H14.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017314263.2"/>
        </source>
    </evidence>
    <sequence length="236" mass="27129" checksum="E0E861DD6D126D53" modified="2023-06-28" version="2">MLPVHSVYPLVLGKTMNSDKHFMDCLRSEMKLHEVDSQNDCDVLIAFVPIVSRAGTDIQAALERIPTEHSYKRVVLVSLHHTFDQDYIAPDSRHSVHRNDVFAVDLLYHEDMGLLKSRTNDEALKRVIKHLGGETPYKAADKTEFIPWLCAVLSSFLFYFVLPEGYSFFHFLILEIFLIIVSGSLSYYSKYLKNSKYHLGKWTPWIFTGMNVVLMLVFLSSTKESVHELNNATEHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q6N0</accession>
    <name>A0A2D0Q6N0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Transformer-2 protein homolog beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Splicing factor, arginine/serine-rich 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Transformer-2 protein homolog B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC108258698</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing. Can either activate or suppress exon inclusion. Acts additively with RBMX to promote exon 7 inclusion of the survival motor neuron SMN2. Activates the splicing of MAPT/Tau exon 10. Alters pre-mRNA splicing patterns by antagonizing the effects of splicing regulators, like RBMX. Binds to the AG-rich SE2 domain in the SMN exon 7 RNA. Binds to pre-mRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the splicing factor SR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017313005.1">
        <property type="nucleotide sequence ID" value="XM_017457516.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q6N0"/>
    <dbReference type="GeneID" id="108258698"/>
    <dbReference type="KEGG" id="ipu:108258698"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12641">
        <property type="entry name" value="RRM_TRA2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000160">
        <property type="entry name" value="Transformer-2 protein homolog beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050441">
        <property type="entry name" value="RBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48034">
        <property type="entry name" value="TRANSFORMER-2 SEX-DETERMINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013510199" description="Transformer-2 protein homolog beta" evidence="19">
        <location>
            <begin position="24"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="20">
        <location>
            <begin position="122"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="22"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="199"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="48"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="99"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="216"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="278"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00053374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00077151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017313005.1"/>
        </source>
    </evidence>
    <sequence length="292" mass="34051" checksum="4D5EF03DB04B5914" modified="2017-12-20" version="1">MHGRFSLWFCCVHLVWIVQESRSRSVSPRGSVKSTSNSPGRSKDGSHRSRSRSKSRSRSRSKSRSRSRSHRSSRRRHSRSRSRRRHSRSRSYSREYRRSRSHSPMSNRRRHVGNRANPDPNCCLGVFGLSLYTTERDLREVFSKYGPLSNVSIVYDQQSRRSRGFAFVYFENKEDSKEAKEQANGMELDGRRIRVDFSITKRPHTPTPGIYMGRPTYGGGGGGGGGGRPSTSSSRSRDCYDRGYDRGYDRGYDRYDDREYYKSYRRRSPSPYYGRGGYRSRSRSRSYSPRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QB35</accession>
    <name>A0A2D0QB35_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Syntaxin-10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC108259528</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Golgi apparatus</location>
            <location evidence="9">trans-Golgi network membrane</location>
            <topology evidence="9">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017314595.1">
        <property type="nucleotide sequence ID" value="XM_017459106.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QB35"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000004424"/>
    <dbReference type="GeneID" id="108259528"/>
    <dbReference type="KEGG" id="ipu:108259528"/>
    <dbReference type="OMA" id="ERASDCC"/>
    <dbReference type="OrthoDB" id="546861at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21443">
        <property type="entry name" value="SNARE_NTD_STX6_STX10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15851">
        <property type="entry name" value="SNARE_Syntaxin6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.90:FF:000004">
        <property type="entry name" value="Syntaxin 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000006">
        <property type="entry name" value="Syntaxin 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015260">
        <property type="entry name" value="Syntaxin-6/10/61_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09177">
        <property type="entry name" value="STX6_10_61_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="150"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017314595.1"/>
        </source>
    </evidence>
    <sequence length="242" mass="28061" checksum="2268F410DFB3B481" modified="2017-12-20" version="1">MDDPFFVVKAEVQKAVLRGRGLCERWVELLQEEEPVSRDELDWSKNELRNCLRAIEWDLEDLQETISIVQSNPGKFRLCENELQERREFVEQTRTAVQEMKQQLSSPSAVAQAEKKNRQALLDTQRNAGLDPHLVSANSRYIQEQQEQQQLIVQEQDEQLELVSGSIRVLKDMSDRIGDELNEQAVMLEEFSEEMEQTGSRMDSVLKKLEKVSHMTSSRRQWCAIGALLIILIIVLILFFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QBE2</accession>
    <name>A0A2D0QBE2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Serine and arginine rich splicing factor 7a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">srsf7a</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017315607.2">
        <property type="nucleotide sequence ID" value="XM_017460118.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QBE2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000028182"/>
    <dbReference type="GeneID" id="108260117"/>
    <dbReference type="KEGG" id="ipu:108260117"/>
    <dbReference type="CTD" id="402972"/>
    <dbReference type="OMA" id="DCYRYNK"/>
    <dbReference type="OrthoDB" id="5970at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000126">
        <property type="entry name" value="Serine and arginine-rich-splicing factor 7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000078">
        <property type="entry name" value="serine/arginine-rich splicing factor 7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48038">
        <property type="entry name" value="RIBONUCLEOPROTEIN RB97D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48038:SF3">
        <property type="entry name" value="SPLICING FACTOR, ARGININE_SERINE-RICH 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="67"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="8">
        <location>
            <begin position="160"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="180"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="143"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="180"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="227"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="290"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017315607.2"/>
        </source>
    </evidence>
    <sequence length="303" mass="35223" checksum="E616BABB04215DC8" modified="2023-02-22" version="2">MYSRTNQDGRPFSPASWTGDEARAPLWNPCLRFRETRARERACVNVTLETSRRRFEMSYSSRSSLDCKVYVGDLGNGAAKGELERAFSYYGPLRSVWVARNPPGFAFVEYEDSRDAEDAVKGMDGKMLCGARIRVEMSNGMSRKSRFGRPSRRQFDPNDRCYQCGETGHYAYDCYRYNKRGGRRSRSRSRSRSRGRRYHSRSRSNERRHRSPSYSRRRSRSASPGRSRSRTPVRRSRSPVRRSRSPARRSRTPVRRASRSRSRSRSRQRARSASRSRSRSVSKKRDSRSRSISPRKSPTPDAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QD64</accession>
    <name>A0A2D0QD64_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Stimulated by retinoic acid gene 6 protein-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC108260686</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017316618.1">
        <property type="nucleotide sequence ID" value="XM_017461129.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QD64"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033229"/>
    <dbReference type="GeneID" id="108260686"/>
    <dbReference type="KEGG" id="ipu:108260686"/>
    <dbReference type="OrthoDB" id="2376984at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034632">
        <property type="term" value="F:retinol transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071939">
        <property type="term" value="P:vitamin A import into cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026612">
        <property type="entry name" value="STRA6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21444">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21444:SF17">
        <property type="entry name" value="STIMULATED BY RETINOIC ACID GENE 6 PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14752">
        <property type="entry name" value="RBP_receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="122"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="200"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="275"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="320"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="385"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="422"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="474"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017316618.1"/>
        </source>
    </evidence>
    <sequence length="614" mass="71390" checksum="A731F93DE4630C13" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QI72</accession>
    <name>A0A2D0QI72_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein NATD1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">N-acetyltransferase domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">natd1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NATD1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017317205.1">
        <property type="nucleotide sequence ID" value="XM_017461716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QI72"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034731"/>
    <dbReference type="GeneID" id="108260961"/>
    <dbReference type="KEGG" id="ipu:108260961"/>
    <dbReference type="CTD" id="256302"/>
    <dbReference type="OrthoDB" id="74247at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000030">
        <property type="entry name" value="NATD1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045057">
        <property type="entry name" value="Gcn5-rel_NAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031165">
        <property type="entry name" value="GNAT_YJDJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31435">
        <property type="entry name" value="PROTEIN NATD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31435:SF9">
        <property type="entry name" value="PROTEIN NATD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14542">
        <property type="entry name" value="Acetyltransf_CG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51729">
        <property type="entry name" value="GNAT_YJDJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="4">
        <location>
            <begin position="19"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00031876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017317205.1"/>
        </source>
    </evidence>
    <sequence length="110" mass="12925" checksum="A7379076EC982C37" modified="2017-12-20" version="1">MAQAAQMNVIEINSPIRVEHDKKRRQFSIRLNGSYDRAVLLYEYVGKKTVDLQHTEVPDAYRGRGIAKHLAKAAMDFVVEEDLRAHLTCWYIQKYVKENPHPHYLEHIIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QKT9</accession>
    <name>A0A2D0QKT9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Interleukin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 11 12" type="primary">il15</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11 12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11 12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
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            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
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                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
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                <person name="Feng J."/>
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                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
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                <person name="Waldbieser G.C."/>
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        </location>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017319311.1"/>
        </source>
    </evidence>
    <sequence length="1512" mass="152651" checksum="0746CA114AD5C6EC" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QP47</accession>
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    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="14">Homodimer; does not require RNA to homodimerize.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the quaking family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017320253.1">
        <property type="nucleotide sequence ID" value="XM_017464764.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QP47"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018590"/>
    <dbReference type="GeneID" id="108263706"/>
    <dbReference type="KEGG" id="ipu:108263706"/>
    <dbReference type="OrthoDB" id="6777263at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048024">
        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014866">
        <property type="term" value="P:skeletal myofibril assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22465">
        <property type="entry name" value="KH-I_Hqk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4010:FF:000001">
        <property type="entry name" value="protein quaking isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000055">
        <property type="entry name" value="protein quaking isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032377">
        <property type="entry name" value="STAR_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF125">
        <property type="entry name" value="KH DOMAIN-CONTAINING RNA-BINDING PROTEIN QKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16544">
        <property type="entry name" value="STAR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="K Homology" evidence="15">
        <location>
            <begin position="80"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00064982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017320253.1"/>
        </source>
    </evidence>
    <sequence length="348" mass="38185" checksum="E91956BDE9381827" modified="2017-12-20" version="1">MVGEMETKEKPKPSPDYLMQLMNDKKLMSSLPNFCGIFNHLERLLDEEISRVRKDMYNDTLNGSTEKRSSELPDAVGPIVQLQEKLYVPVKEYPDFNFVGRILGPRGLTAKQLEAETGCKIMVRGKGSMRDKKKEEQNRGKPNWEHLNEDLHVLITVEDAQNRAEIKLKRAVEEVNKLLVPAAEGEDSLKKMQLMELAILNGTYRDANIKSPALAFSLAASAQAPRIISGPAPVLPPAALRTPAPTGPTIMPLIRQIQTVVPNGSAHAHAAAATLVPPGPESAGLIYAAPYEYPYTLAPATSILEYPIDSSGVLASSKAEELPLYLGFQTCHFPITVQRAEPAGRPPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QYQ6</accession>
    <name>A0A2D0QYQ6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">MyoD family inhibitor isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">zgc:113363</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4 5">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MDFI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017323444.1">
        <property type="nucleotide sequence ID" value="XM_017467955.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017323445.1">
        <property type="nucleotide sequence ID" value="XM_017467956.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QYQ6"/>
    <dbReference type="GeneID" id="108265528"/>
    <dbReference type="KEGG" id="ipu:108265528"/>
    <dbReference type="OMA" id="HASFKTD"/>
    <dbReference type="OrthoDB" id="9046739at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026134">
        <property type="entry name" value="MDFI/MDFIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15304">
        <property type="entry name" value="MYOD FAMILY INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15304:SF4">
        <property type="entry name" value="MYOD FAMILY INHIBITOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15316">
        <property type="entry name" value="MDFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="93"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="93"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017323444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017323445.1"/>
        </source>
    </evidence>
    <sequence length="251" mass="26725" checksum="60656C1E9E1CAB95" modified="2017-12-20" version="1">MPHKETEAMDVQQHSGKDRTHRNHLIQPESRVAEQDITQQPDPLSAKLPRANGIGYGGSNDSLTDIVITNENALLLPNHVPVIVHKLDKETPSPLNGLSSTDTVPNGSPPVCTPPTASTGQRAPDSAHIPKANRHNKPHKKRPPSTHKSHQSLKTSASQIQEVAGDDCCAHCVLACLFCELVPMCSALAQCLACGMECDALCCCGEACGDLAFCSGDPCTAVLDCAILEDCCQSSDCLEICLECCSICFPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QYZ1</accession>
    <name>A0A2D0QYZ1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Perilipin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">plin3</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the perilipin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017323618.1">
        <property type="nucleotide sequence ID" value="XM_017468129.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QYZ1"/>
    <dbReference type="GeneID" id="108265600"/>
    <dbReference type="CTD" id="10226"/>
    <dbReference type="OrthoDB" id="376826at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010890">
        <property type="term" value="P:positive regulation of triglyceride storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.340">
        <property type="entry name" value="Flagellar protein FliS"/>
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    </dbReference>
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        <property type="entry name" value="Perilipin, alpha-beta domain"/>
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        <property type="entry name" value="Perilipin"/>
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        <property type="entry name" value="PERILIPIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR14024:SF11">
        <property type="entry name" value="PERILIPIN-3"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF03036">
        <property type="entry name" value="Perilipin"/>
        <property type="match status" value="1"/>
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    </dbReference>
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    <keyword evidence="7" id="KW-0675">Receptor</keyword>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
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            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
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        <location>
            <begin position="292"/>
            <end position="318"/>
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            <begin position="1"/>
            <end position="10"/>
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    </feature>
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        <location>
            <begin position="206"/>
            <end position="215"/>
        </location>
    </feature>
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        <location>
            <begin position="292"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006311"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017323618.1"/>
        </source>
    </evidence>
    <sequence length="430" mass="45929" checksum="85F819E95634D146" modified="2017-12-20" version="1">MAENGKHGEMDTETPPASQETQSVMSRVSNLALVSSACDAVSSAYSSTKESVPVLRGVMDAAESGVRTLSTAASSGTKPILNKLEPQIAMVNEYAMKGLDKMEENLPVLHQPADKLVSETVGKVYQCVSGAKDAVVGAMMGGVEMTRTALSGGINTVMGSRVGQMVSSGMGLSLNQSEDRVEQKLPLTETELAAVAEPPPKDEVEVQTSLSSEGVASQSSAPSYFVRLGKLTAQVQERALELSLIKASAARDATHKAVSQITSTLDLLENLRGANALVGAPEQLLKRWREWRQEGQEEHGQGHGAESTEEDGGEDGKENGELEWRALSMVRSLSAQLRVACSGVVSSVQGLPSAVQEQLLNARHSAEELHSSLGKTRTVTPLLLQQSRNQLTQVRQSLDGVMDYLLNNTPLNWLVGPFSPQLVEKRPSAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R0F0</accession>
    <name>A0A2D0R0F0_ICTPU</name>
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        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
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        </recommendedName>
    </protein>
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        <name evidence="13" type="primary">ulk1a</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="RefSeq" id="XP_017323501.1">
        <property type="nucleotide sequence ID" value="XM_017468012.3"/>
    </dbReference>
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    <dbReference type="GeneID" id="108265551"/>
    <dbReference type="KEGG" id="ipu:108265551"/>
    <dbReference type="CTD" id="100170793"/>
    <dbReference type="OrthoDB" id="346907at2759"/>
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        <property type="component" value="Chromosome 5"/>
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        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:autophagosome assembly"/>
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        <property type="term" value="P:autophagy of mitochondrion"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:axon extension"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of collateral sprouting"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:response to starvation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="serine/threonine-protein kinase ULK2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="serine/threonine-protein kinase unc-51 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048941">
        <property type="entry name" value="ATG1-like_MIT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022708">
        <property type="entry name" value="Atg1-like_tMIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF19">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ULK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12063">
        <property type="entry name" value="ATG1-like_MIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21127">
        <property type="entry name" value="ATG1-like_MIT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0072">Autophagy</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="9"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="276"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="440"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="550"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="288"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="326"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="473"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017323501.1"/>
        </source>
    </evidence>
    <sequence length="936" mass="103111" checksum="E08F8831B01575BB" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R0H9</accession>
    <name>A0A2D0R0H9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dickkopf-related protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC108265560</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017323532.1">
        <property type="nucleotide sequence ID" value="XM_017468043.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R0H9"/>
    <dbReference type="GeneID" id="108265560"/>
    <dbReference type="KEGG" id="ipu:108265560"/>
    <dbReference type="OrthoDB" id="8882232at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.80.10">
        <property type="entry name" value="Lipase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013510428" evidence="2">
        <location>
            <begin position="18"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017323532.1"/>
        </source>
    </evidence>
    <sequence length="183" mass="20289" checksum="660CDECDF2BBD4EA" modified="2017-12-20" version="1">MWTPAVFALCLTSAVLCLDSNEIRSSREIITDPEPLFTRNTTAMPSEKVTVNVSVHNNVRRRQQNPECTNQQGNRCKGTKKRQGRKNKPDDTATEKGAKRGECVRSRDCEEGWCCVQFPSGGRCQRVPQEGEMCTLGGPPKSKSRRIFKRCACTAGLNCLRKPGSPKGQGECGVHGREQDNPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R210</accession>
    <name>A0A2D0R210_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">aspartate transaminase</fullName>
            <ecNumber evidence="4">2.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">got1l1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017323810.1">
        <property type="nucleotide sequence ID" value="XM_017468321.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R210"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000014803"/>
    <dbReference type="GeneID" id="108265709"/>
    <dbReference type="KEGG" id="ipu:108265709"/>
    <dbReference type="CTD" id="137362"/>
    <dbReference type="OMA" id="PGGLYCC"/>
    <dbReference type="OrthoDB" id="6752799at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006532">
        <property type="term" value="P:aspartate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF36">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE, CYTOPLASMIC 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="8">
        <location>
            <begin position="33"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017323810.1"/>
        </source>
    </evidence>
    <sequence length="412" mass="45681" checksum="D8D7CB59A885F868" modified="2017-12-20" version="1">MASCPSAPRDTPVAAINTEEKLLEDFQRDSHPDKVCLACREYVGEDGHPIVNPLIRKIKQQIATDPTLIPEYPPPLGIPEFSSRASQLALGRDSPAILENRVLVVQTVGSSGAVRLGAELLKRYFCVSSSWAGDVLLSSPCDESLPGIFKAAGIEAVKRYRYWDSGGRKVCVEEMLEDLEKAPEQSTVVLSASGHSPTGADLSQDEWKRVAEILVKRRLLPFFLLPAQGLCSRDIDQDSWALRYCVSLGMELLCAQSFSHNFSLYGERVAHLLCVVKENATLLAVQFQAEKIVQTLWSRPPVEGARVVTTVLSNPAHMVEWQEGLKRTVERCMLIKERLREKLRLLGSPGTWEHLIKSAGLYCCIGLSVQQVEFLVKQRHVYLLPGGCFNVSAINSNNLDYVAESLHLILTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R6M5</accession>
    <name>A0A2D0R6M5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome c oxidase copper chaperone</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC108266537</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Copper metallochaperone essential for the assembly of the mitochondrial respiratory chain complex IV (CIV), also known as cytochrome c oxidase. Binds two copper ions and delivers them to the metallochaperone SCO1 which transports the copper ions to the Cu(A) site on the cytochrome c oxidase subunit II (MT-CO2/COX2).</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with COA1. Interacts with the chaperone CHCHD4; this is important for correct folding and the formation of disulfide bonds that stabilize the structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the COX17 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017325475.1">
        <property type="nucleotide sequence ID" value="XM_017469986.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R6M5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005207"/>
    <dbReference type="GeneID" id="108266537"/>
    <dbReference type="KEGG" id="ipu:108266537"/>
    <dbReference type="OMA" id="HKACMRK"/>
    <dbReference type="OrthoDB" id="1915887at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016531">
        <property type="term" value="F:copper chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1130:FF:000001">
        <property type="entry name" value="cytochrome c oxidase copper chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1130">
        <property type="entry name" value="CytochromE C oxidase copper chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007745">
        <property type="entry name" value="Cyt_c_oxidase_Cu-chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16719">
        <property type="entry name" value="CYTOCHROME C OXIDASE COPPER CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16719:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE COPPER CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05051">
        <property type="entry name" value="COX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7 14" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="14">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00065132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR607745-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017325475.1"/>
        </source>
    </evidence>
    <sequence length="63" mass="6725" checksum="7DDA7F0F97C9FD48" modified="2017-12-20" version="1">MSSIAAASVEPTAAEEKKPLKPCCACPETKKARDACIIEKGEESCTGLIEAHKECMRALGFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RAK5</accession>
    <name>A0A2D0RAK5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Chromodomain-helicase-DNA-binding protein 8</fullName>
            <shortName evidence="15">CHD-8</shortName>
            <ecNumber evidence="15">3.6.4.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">ATP-dependent helicase CHD8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21 22" type="primary">chd8</name>
        <name evidence="15" type="synonym">CHD8</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21 22" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21 22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">ATP-dependent chromatin-remodeling factor, it slides nucleosomes along DNA; nucleosome sliding requires ATP. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. May also act as a transcription activator by participating in efficient U6 RNA polymerase III transcription.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of some MLL1/MLL complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the SNF2/RAD54 helicase family. CHD8 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="15"/>
    <dbReference type="RefSeq" id="XP_017327592.1">
        <property type="nucleotide sequence ID" value="XM_017472103.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053537280.1">
        <property type="nucleotide sequence ID" value="XM_053681305.1"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000029429"/>
    <dbReference type="GeneID" id="108267737"/>
    <dbReference type="KEGG" id="ipu:108267737"/>
    <dbReference type="CTD" id="57680"/>
    <dbReference type="OMA" id="CEWASME"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071339">
        <property type="term" value="C:MLL1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002039">
        <property type="term" value="F:p53 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18668">
        <property type="entry name" value="CD1_tandem_CHD5-9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18663">
        <property type="entry name" value="CD2_tandem_CHD5-9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000001">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 8 isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000003">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 8 isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000015">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.120">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03071">
        <property type="entry name" value="CHD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006576">
        <property type="entry name" value="BRK_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037259">
        <property type="entry name" value="BRK_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051493">
        <property type="entry name" value="CHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034724">
        <property type="entry name" value="CHD8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056342">
        <property type="entry name" value="HTH_CHD6-9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46850">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46850:SF1">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07533">
        <property type="entry name" value="BRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23078">
        <property type="entry name" value="HTH_CHD6-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00592">
        <property type="entry name" value="BRK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160481">
        <property type="entry name" value="BRK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-0010">Activator</keyword>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 15" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="10 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="15" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 15" id="KW-0678">Repressor</keyword>
    <keyword evidence="12 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 15" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 15" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="Chromo" evidence="17">
        <location>
            <begin position="705"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="17">
        <location>
            <begin position="787"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="886"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="1201"/>
            <end position="1352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="524"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="569"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1470"/>
            <end position="1512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="2096"/>
            <end position="2200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="2299"/>
            <end position="2324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="2353"/>
            <end position="2373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="536"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="615"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="631"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1482"/>
            <end position="1492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="2115"/>
            <end position="2125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="2145"/>
            <end position="2159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="2353"/>
            <end position="2362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="2363"/>
            <end position="2373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
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                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
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                <person name="Feng J."/>
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                <person name="Duke M.V."/>
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                <person name="Xie Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="16">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
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                <person name="Bao L."/>
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                <person name="Li Y."/>
                <person name="Jiang C."/>
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                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
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                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
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                <person name="Chen A."/>
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                <person name="Yuan Z."/>
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                <person name="Peatman E."/>
                <person name="Lu J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">SDA103</strain>
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        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT HYPOPHOSPHITE DIOXYGENASE-LIKE GENE A2 [PROVISIONAL]-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20883">
        <property type="entry name" value="PHYTANOYL-COA DIOXYGENASE DOMAIN CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05721">
        <property type="entry name" value="PhyH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017328882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017328884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017328885.1"/>
        </source>
    </evidence>
    <sequence length="278" mass="31282" checksum="5609FF1996C651B9" modified="2017-12-20" version="1">MGIDRAEVKTLYEKQGYVTSIPILSPEELQQARDAFAELERKFGEDYTSYSLHNVHMKYDWVMALIKHPRVLDVITAVLGNDVLLLDSRFICKYPVSKEIKTQAGNDIIAHNPEKKESDQENEPGLPYVAWHQDMKYWGLDGGPVVSVWLALDDSQEENGALQVIPGTHCSGLLPHRQAKRQGNMLTVNQEIPEELVEKDKALLCPLLAGQMSIHDGLLVHASDPNTSGKRRCGFVIRYVPTAAYPIQDPERPRSFHATSVVSGHDKYNHFSSQKPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RF92</accession>
    <name>A0A2D0RF92_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Sema domain, transmembrane domain (TM), and cytoplasmic domain, (Semaphorin) 6E isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">sema6e</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017328570.1">
        <property type="nucleotide sequence ID" value="XM_017473081.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RF92"/>
    <dbReference type="GeneID" id="108268218"/>
    <dbReference type="CTD" id="556743"/>
    <dbReference type="OrthoDB" id="9988752at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11242">
        <property type="entry name" value="Sema_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000026">
        <property type="entry name" value="Sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000013">
        <property type="entry name" value="semaphorin-6D isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF11">
        <property type="entry name" value="SEMAPHORIN-6C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="7 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012474672" evidence="8">
        <location>
            <begin position="28"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="658"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="9">
        <location>
            <begin position="29"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="355"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="583"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="797"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="913"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="589"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="614"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="913"/>
            <end position="927"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017328570.1"/>
        </source>
    </evidence>
    <sequence length="1055" mass="116630" checksum="FD8BE31C645FB143" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RME5</accession>
    <name>A0A2D0RME5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">(E2-independent) E3 ubiquitin-conjugating enzyme FATS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC108269669</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017331120.1">
        <property type="nucleotide sequence ID" value="XM_017475631.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RME5"/>
    <dbReference type="GeneID" id="108269669"/>
    <dbReference type="KEGG" id="ipu:108269669"/>
    <dbReference type="OrthoDB" id="8899035at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046599">
        <property type="term" value="P:regulation of centriole replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029299">
        <property type="entry name" value="ALMS_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21553:SF24">
        <property type="entry name" value="(E2-INDEPENDENT) E3 UBIQUITIN-CONJUGATING ENZYME FATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21553">
        <property type="entry name" value="ALMS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15309">
        <property type="entry name" value="ALMS_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ALMS motif" evidence="4">
        <location>
            <begin position="29"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017331120.1"/>
        </source>
    </evidence>
    <sequence length="157" mass="18668" checksum="BB6BEB63B6FC52EE" modified="2017-12-20" version="1">MVPRPQECVVSSHLLNNCTYGSKITDQLQLLSLKEALQFFRPDFILRSQRRVHRLEQRARERRGLQPGDSTVLLQTTNRRQNCTKPHPLSDNLFKPKDRVISGKEMHLRSRRIYNKLPEVTKKKEEERRRLVLQTNRLRAEAFKKKLLDQLLQRNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RV48</accession>
    <name>A0A2D0RV48_ICTPU</name>
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        <submittedName>
            <fullName evidence="16">Tripartite motif-containing protein 66 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">trim66</name>
    </gene>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017334369.1">
        <property type="nucleotide sequence ID" value="XM_017478880.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RV48"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005699"/>
    <dbReference type="GeneID" id="108271337"/>
    <dbReference type="KEGG" id="ipu:108271337"/>
    <dbReference type="CTD" id="9866"/>
    <dbReference type="OMA" id="PQDFQWP"/>
    <dbReference type="OrthoDB" id="1870062at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19794">
        <property type="entry name" value="Bbox2_TRIM66-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05502">
        <property type="entry name" value="Bromo_tif1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000123">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003649">
        <property type="entry name" value="Bbox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037372">
        <property type="entry name" value="TRIM66_Bbox1_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45915">
        <property type="entry name" value="TRANSCRIPTION INTERMEDIARY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45915:SF7">
        <property type="entry name" value="TRIPARTITE MOTIF-CONTAINING PROTEIN 66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25287">
        <property type="entry name" value="zf-B_box_Trim66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00502">
        <property type="entry name" value="BBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="14">
        <location>
            <begin position="68"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="13">
        <location>
            <begin position="824"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="12">
        <location>
            <begin position="910"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="351"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="595"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1032"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="351"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="407"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="475"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="516"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="602"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="660"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="688"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="738"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="752"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1036"/>
            <end position="1054"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017334369.1"/>
        </source>
    </evidence>
    <sequence length="1054" mass="116842" checksum="FEDF605CE1BDA077" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RVC8</accession>
    <name>A0A2D0RVC8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ribonuclease P protein subunit p21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpp21</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the eukaryotic/archaeal RNase P protein component 4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017334449.1">
        <property type="nucleotide sequence ID" value="XM_017478960.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RVC8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000037218"/>
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    <dbReference type="KEGG" id="ipu:108271386"/>
    <dbReference type="CTD" id="79897"/>
    <dbReference type="OMA" id="DPKHLLW"/>
    <dbReference type="OrthoDB" id="128536at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007175">
        <property type="entry name" value="Rpr2/Snm1/Rpp21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rpr2"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017334449.1"/>
        </source>
    </evidence>
    <sequence length="154" mass="17328" checksum="CEE2291CDF6F9480" modified="2017-12-20" version="1">MAANIKDKEAFQRLNFLYQAAHCVLAQNPENTELARFYCFTQKTIAKRLVLRQDPSVKRTMCKKCCALLIPGVTATVRQRSSLRQRATLIRCLGCGQTKKFPNNPKHQLWVDQSEAQLENQLQPEACPSSKALTQKDKPDKCSTVVPVIHDAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RXG6</accession>
    <name>A0A2D0RXG6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Protein WWC3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">wwc3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017335192.1">
        <property type="nucleotide sequence ID" value="XM_017479703.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RXG6"/>
    <dbReference type="GeneID" id="108271849"/>
    <dbReference type="KEGG" id="ipu:108271849"/>
    <dbReference type="CTD" id="55841"/>
    <dbReference type="OrthoDB" id="2020426at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019900">
        <property type="term" value="F:kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046621">
        <property type="term" value="P:negative regulation of organ growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035330">
        <property type="term" value="P:regulation of hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000001">
        <property type="entry name" value="Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000041">
        <property type="entry name" value="WW and C2 domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051105">
        <property type="entry name" value="WWC/KIBRA_Hippo_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057747">
        <property type="entry name" value="WWC1_hairpin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14791">
        <property type="entry name" value="BOMB/KIRA PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14791:SF25">
        <property type="entry name" value="PROTEIN WWC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25802">
        <property type="entry name" value="WWC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WW" evidence="10">
        <location>
            <begin position="15"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="10">
        <location>
            <begin position="62"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="9">
        <location>
            <begin position="679"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="441"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="523"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="816"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1053"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1089"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="318"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="150"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="523"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="850"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1096"/>
            <end position="1106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017335192.1"/>
        </source>
    </evidence>
    <sequence length="1154" mass="130056" checksum="93C026D8E60D85CC" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RYI7</accession>
    <name>A0A2D0RYI7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Divergent protein kinase domain 1C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">dipk1c</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DIPK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017335559.1">
        <property type="nucleotide sequence ID" value="XM_017480070.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RYI7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000445"/>
    <dbReference type="GeneID" id="108272009"/>
    <dbReference type="KEGG" id="ipu:108272009"/>
    <dbReference type="CTD" id="125704"/>
    <dbReference type="OMA" id="LHYDRGK"/>
    <dbReference type="OrthoDB" id="8543887at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022049">
        <property type="entry name" value="FAM69_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029244">
        <property type="entry name" value="FAM69_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21093:SF2">
        <property type="entry name" value="DIVERGENT PROTEIN KINASE DOMAIN 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21093">
        <property type="entry name" value="DIVERGENT PROTEIN KINASE DOMAIN 1C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12260">
        <property type="entry name" value="PIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14875">
        <property type="entry name" value="PIP49_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01299">
        <property type="entry name" value="PIP49_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="FAM69 N-terminal" evidence="10">
        <location>
            <begin position="23"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017335559.1"/>
        </source>
    </evidence>
    <sequence length="454" mass="51780" checksum="DF67B411CC54F7FA" modified="2017-12-20" version="1">MFRAVCRAAGLGRGGLGLRWLRARVRRKSAVLFILLWLASWLFLNGLLFVHTTMFSDFCTDDKSRRILQRLCEEYQQGVLTGDLCEDLCVGGRVEYKRCLYYENGKKVMAASWRGVPVVLKSKLENFSSYEALALLEYQDTADDVGAEEELSPLDVVFYAALQIKNLLGLEAGSNLTLPRLWGQKLKKRSQSYSRAELASLWSLLQQEEYAFLRVMQDLTDHVAKVLGSCGHFYAVEYLAAGHAWDQNIFSLEEFSQSPKSEEGQTLTEIDMVPRVALSFLDMVDHFENDFSHHLHLCDIKPENFAIRKDLTVAAIDVDMTFFEPKMQDILEQNCTNDHDCNFFDCISKCDTKRNKCGSKRKNSNLQVICEKIFRSWFSPTLLGDKAGRPLQVELQQAVQDCAETEGGEDAERARNIRLHLKGLLTQLVQEDSANHQSRKQAHAKRRIHTALNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S1F9</accession>
    <name>A0A2D0S1F9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ddx3xb</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="RefSeq" id="XP_017336593.1">
        <property type="nucleotide sequence ID" value="XM_017481104.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S1F9"/>
    <dbReference type="GeneID" id="108272611"/>
    <dbReference type="KEGG" id="ipu:108272611"/>
    <dbReference type="CTD" id="30116"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18051">
        <property type="entry name" value="DEADc_DDX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000160">
        <property type="entry name" value="ATP-dependent RNA helicase DDX3X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10 16" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="14">
        <location>
            <begin position="246"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="277"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="484"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="23"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="157"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="686"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="9">
        <location>
            <begin position="246"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="114"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="697"/>
            <end position="713"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017336593.1"/>
        </source>
    </evidence>
    <sequence length="744" mass="81816" checksum="C960AED2B53F5186" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S2A4</accession>
    <name>A0A2D0S2A4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC108272419</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108272419</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017336325.1">
        <property type="nucleotide sequence ID" value="XM_017480836.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S2A4"/>
    <dbReference type="GeneID" id="108272419"/>
    <dbReference type="KEGG" id="ipu:108272419"/>
    <dbReference type="OrthoDB" id="10260387at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="90"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="181"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017336325.1"/>
        </source>
    </evidence>
    <sequence length="337" mass="38007" checksum="A36DBA5C5152FBF6" modified="2017-12-20" version="1">MAQNYSSEVVLKNTAEAVIIPLKNGISSLNGVYEALIKADVDPITGQCSNYNYIRQQIVQAHQHLQRSEQVANSGLKSLDESLERFTEDEGKLECDINDTEKTLDNLRTKQAFNQKLLRECQGSLELARTKLNSTKQILQEQESRKHNAEIVTGVGAGLMVIPFIGWIAGSAMMIGGAVEMDQAENAVSEAEEEERRSYNEVKNYEHKVSDYESKISHTSHDISQKNDKLKMAREGIQEVKKQIQAVADFQSKARRAVQFLGVLSGRASVAEHQTRRFILQEPVMKVMEDVMNAMKEITGNEFLYNKDMMRLIDQMKENNLRLAAICASEKNAVDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S320</accession>
    <name>A0A2D0S320_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Abl interactor 1a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">abi1a</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Filopodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ABI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017337132.1">
        <property type="nucleotide sequence ID" value="XM_017481643.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S320"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003097"/>
    <dbReference type="GeneID" id="108272842"/>
    <dbReference type="KEGG" id="ipu:108272842"/>
    <dbReference type="CTD" id="393711"/>
    <dbReference type="OMA" id="HGVKEWH"/>
    <dbReference type="OrthoDB" id="5971719at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030175">
        <property type="term" value="C:filopodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031209">
        <property type="term" value="C:SCAR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000002">
        <property type="entry name" value="abl interactor 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028457">
        <property type="entry name" value="ABI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012849">
        <property type="entry name" value="Abl-interactor_HHR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10460:SF2">
        <property type="entry name" value="ABL INTERACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10460">
        <property type="entry name" value="ABL INTERACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07815">
        <property type="entry name" value="Abi_HHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="14">
        <location>
            <begin position="45"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="445"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="159"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="388"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="161"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="226"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="248"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="392"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017337132.1"/>
        </source>
    </evidence>
    <sequence length="507" mass="54766" checksum="270E316B45BB6A5A" modified="2017-12-20" version="1">MAELQMLLEEEIPAGKSALLESYQNLSRVADYCENNYVQAQDKRKALEETKAYATQSLASVAYQINALANNVLQLLDIQASQLRRMESSINHISQTVDIHKEKVARREIGILTTNKNTTRTHKIIAPANIERPVRYIRKPIDYTMLDDVGHGVKWLKAKQHGNNQSVRGGTLSRTNPPTQKPPSPPMAGRGTLGRNTSYKTLEPVKPPTVPNDYMTSPARLGTHSPGRTASLSQRPRTHSGSSGGSGSRENSGGSSIGIPIAVPTPSIPNSGPVPSMFAPPPPPPPPMFGAPMAIGLGPAPVSQFGTVSRQMSRHNSTTSSMSTVSATGTYRRAPSLSSQLPLQHTNVNGAAPAAFTHNSMSVAPPPPPMLIPQIPLTGFVARVQENIADTPTPPPPPPPDDVPIFDEAPPPPPPPPVDYEEEEAAVVQYSDPYADGDPQWAPKNYIEKVVAIYDYTKDKDDELSFTEGAIIYIIKKNDDGWFEGVSSGVTGLFPGNYVEPIMHYAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S3F6</accession>
    <name>A0A2D0S3F6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhomboid-related protein 3</fullName>
            <ecNumber evidence="7">3.4.21.105</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">rhbdl3</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12 13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in regulated intramembrane proteolysis and the subsequent release of functional polypeptides from their membrane anchors.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.</text>
            <dbReference type="EC" id="3.4.21.105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="7"/>
    <dbReference type="RefSeq" id="XP_017337287.1">
        <property type="nucleotide sequence ID" value="XM_017481798.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017337288.1">
        <property type="nucleotide sequence ID" value="XM_017481799.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S3F6"/>
    <dbReference type="GeneID" id="108272931"/>
    <dbReference type="KEGG" id="ipu:108272931"/>
    <dbReference type="CTD" id="162494"/>
    <dbReference type="GeneTree" id="ENSGT00940000158838"/>
    <dbReference type="OrthoDB" id="418595at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000002">
        <property type="entry name" value="Rhomboid, veinlet-like 3 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017213">
        <property type="entry name" value="Peptidase_S54_rhomboid_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051739">
        <property type="entry name" value="Rhomboid_IM_Serine_Proteases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45840">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45840:SF5">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037470">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="340"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="373"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="35"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR037470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR037470-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017337287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017337288.1"/>
        </source>
    </evidence>
    <sequence length="407" mass="45345" checksum="4231F2B480B2C500" modified="2017-12-20" version="1">MTTGARANSSPAVAAHAEGDEIEVLESGETETGSHPDTQWKSLFDKYDPESSGFISAERLRDLLETHGSELDTHKLEVLLALANGNAEGKVRYQDFMNLMSNKRSNSFRRAIQQGGRQLKSSSLREEVGLGLSQRLVRHVAYETLPREVDRKWYFDSYTYCPPPWLILTITIAEMAVFVYYGLLLDRWVLQVSSPSFLKSPLPYHPQRRAQACRYFSYIFMHAGIEHLGLNMAMQLLVGVPLEMVHGAARIGLIYLCGVLAGDGSLVVSVVDMTAPVVGSSGGVYALVSAHLANVVMNWSGMKCQFKLFRMAMALVCMSVEFGRAVWLRFYPPAVPPCPNPSFCAHLGGVAVGLTLGVVVLQNYEQRLHEQFLFWIFMSVYGLFVLFGIFWNVFAYSLLDVKLPPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S7H8</accession>
    <name>A0A2D0S7H8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Homeobox protein Hox-B8b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">hoxb8b</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Antp homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017338694.1">
        <property type="nucleotide sequence ID" value="XM_017483205.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S7H8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000028857"/>
    <dbReference type="GeneID" id="108273740"/>
    <dbReference type="KEGG" id="ipu:108273740"/>
    <dbReference type="CTD" id="30420"/>
    <dbReference type="OMA" id="SHAPYQH"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000072">
        <property type="entry name" value="Homeobox protein Hox-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050948">
        <property type="entry name" value="Antp_homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46166">
        <property type="entry name" value="HOMEOBOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46166:SF2">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="139"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="141"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="199"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="199"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017338694.1"/>
        </source>
    </evidence>
    <sequence length="239" mass="27864" checksum="11DAEEE12EC0DCA0" modified="2017-12-20" version="1">MSSYFVNSLFSKFKGSDALRPNYYECSGYTQELAGRPSVLYGHSTGSTFQHTAQLPEFYHHGASTFSHAPYQHSQYHGETAANLFAQETLQRQTFFGTQDTDLAQFGDYSVKASYISDDLESAETCPTQLFPWMRPQATGRRRGRQTYSRYQTLELEKEFLFNPYLNRKRRVEVSRALCLTERQVKIWFQNRRMKWKKENNKDKFPSSRAEQEQIERQRCECVDDSGKRTSEVADSEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SDB7</accession>
    <name>A0A2D0SDB7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein phosphatase 1 regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">zmp:0000001167</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Regulates myosin phosphatase activity. Augments Ca(2+) sensitivity of the contractile apparatus.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12B mediates binding to myosin. Isoform 3 and isoform 4 bind PPP1R12A, but not isoform 1 of PPP1R12B itself. Binds IL16.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">PP1 comprises a catalytic subunit, and one or several targeting or regulatory subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017340461.1">
        <property type="nucleotide sequence ID" value="XM_017484972.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SDB7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013359"/>
    <dbReference type="GeneID" id="108274702"/>
    <dbReference type="KEGG" id="ipu:108274702"/>
    <dbReference type="CTD" id="571837"/>
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    <dbReference type="Proteomes" id="UP000221080">
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            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC). Recruited by UPF3B associated with the EJC core at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF3B stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017341091.1">
        <property type="nucleotide sequence ID" value="XM_017485602.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053541551.1">
        <property type="nucleotide sequence ID" value="XM_053685576.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SF43"/>
    <dbReference type="GeneID" id="108275048"/>
    <dbReference type="KEGG" id="ipu:108275048"/>
    <dbReference type="CTD" id="26019"/>
    <dbReference type="OrthoDB" id="27832at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000014">
        <property type="entry name" value="Putative regulator of nonsense transcripts 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.80.160:FF:000002">
        <property type="entry name" value="Putative regulator of nonsense transcripts 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000015">
        <property type="entry name" value="regulator of nonsense transcripts 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000023">
        <property type="entry name" value="regulator of nonsense transcripts 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.80.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039762">
        <property type="entry name" value="Nmd2/UPF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007193">
        <property type="entry name" value="Upf2/Nmd2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 2 UP-FRAMESHIFT SUPPRESSOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839:SF7">
        <property type="entry name" value="REGULATOR OF NONSENSE TRANSCRIPTS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04050">
        <property type="entry name" value="Upf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="MIF4G" evidence="12">
        <location>
            <begin position="171"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G" evidence="12">
        <location>
            <begin position="572"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G" evidence="12">
        <location>
            <begin position="776"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="491"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1027"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1233"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="491"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1032"/>
            <end position="1082"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00080859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017341091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_053541551.1"/>
        </source>
    </evidence>
    <sequence length="1279" mass="148387" checksum="0A36CA6D57512DC7" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SGF2</accession>
    <name>A0A2D0SGF2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Nicotinamide-nucleotide adenylyltransferase</fullName>
            <ecNumber evidence="20">2.7.7.1</ecNumber>
            <ecNumber evidence="20">2.7.7.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">nmnat1</name>
        <name evidence="23" type="synonym">nmna1</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>beta-nicotinamide D-ribonucleotide + ATP + H(+) = diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21360"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="EC" id="2.7.7.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:21361"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="18">
            <dbReference type="Rhea" id="RHEA:21362"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>nicotinate beta-D-ribonucleotide + ATP + H(+) = deamido-NAD(+) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22860"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="EC" id="2.7.7.18"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:22861"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:22862"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 20">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; NAD(+) biosynthesis; deamido-NAD(+) from nicotinate D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Homohexamer. Interacts with ADPRT/PARP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 20">Belongs to the eukaryotic NMN adenylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.1" evidence="20"/>
    <dbReference type="EC" id="2.7.7.18" evidence="20"/>
    <dbReference type="RefSeq" id="XP_017341789.1">
        <property type="nucleotide sequence ID" value="XM_017486300.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SGF2"/>
    <dbReference type="GeneID" id="100528963"/>
    <dbReference type="CTD" id="64802"/>
    <dbReference type="OrthoDB" id="422187at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00332"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004515">
        <property type="term" value="F:nicotinate-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd09286">
        <property type="entry name" value="NMNAT_Eukarya"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000101">
        <property type="entry name" value="Nicotinamide-nucleotide adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051182">
        <property type="entry name" value="Euk_NMN_adenylyltrnsfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005248">
        <property type="entry name" value="NadD/NMNAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045094">
        <property type="entry name" value="NMNAT_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00482">
        <property type="entry name" value="nicotinate (nicotinamide) nucleotide adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12039">
        <property type="entry name" value="NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12039:SF21">
        <property type="entry name" value="NICOTINAMIDE_NICOTINIC ACID MONONUCLEOTIDE ADENYLYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 20" id="KW-0520">NAD</keyword>
    <keyword evidence="11 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 20 23" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8 20" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="21">
        <location>
            <begin position="39"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00064648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU362021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017341789.1"/>
        </source>
    </evidence>
    <sequence length="299" mass="34329" checksum="9CD09A4836ECBBC0" modified="2017-12-20" version="1">MWLILGSPFSSHSMSRISSMLTFMTSPMALQERIRVVLLACGSFNPITNMHLRMFELARDHLEDTGRYRVVKGIISPVGDAYKKKGLIEACHRVEMAKLATENSSWISVDDWESQQAEWVETAKVIRHHHAELSSAVESLDEVDTVKFPKKRRVEENEESSSDHNRSETLQLKLLCGADVLESFSIPNLWKKEDIAEIVGRFGLVCITRSGCDAERFVYQSDMLHKYRKNIHIVREWVTNEISATHVRRAVCRGQSVRYLLPEPVVRYIQDQHLYSAESEQKNADVVLAPLQRYNRAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SHA0</accession>
    <name>A0A2D0SHA0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Interleukin-23 subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108275681</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017342084.1">
        <property type="nucleotide sequence ID" value="XM_017486595.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SHA0"/>
    <dbReference type="GeneID" id="108275681"/>
    <dbReference type="KEGG" id="ipu:108275681"/>
    <dbReference type="OrthoDB" id="9904313at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010831">
        <property type="entry name" value="IL-23_alpha"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16649">
        <property type="entry name" value="IL23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012858771" evidence="1">
        <location>
            <begin position="20"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017342084.1"/>
        </source>
    </evidence>
    <sequence length="183" mass="20326" checksum="CBD2BCD6E65C5BC2" modified="2017-12-20" version="1">MNPFQQLALIAVMLVCALGAPVRVAETNYTDGMTLVLGLNEKARQLYQEVETPSEVAAVSDPPLMIRAEDKCDPDSLKTNPETCLSRVVLALKNYSRIFGESGIFQGQNTKCDKWRANATAIAVVTEQILEALKEPSETPTPMMMANWMTEGYLCRDSVERLYSFSIMTARVFSHLSSLQQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SLG2</accession>
    <name>A0A2D0SLG2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="9">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">edem2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the endoplasmic reticulum-associated degradation (ERAD) pathway that targets misfolded glycoproteins for degradation in an N-glycan-dependent manner. May initiate ERAD by promoting the first mannose trimming step of ERAD substrates, from Man9GlcNAc2 to Man8GlcNAc2. Seems to recognize and bind to exposed hydrophobic regions in target proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="9"/>
    <dbReference type="RefSeq" id="XP_017343336.1">
        <property type="nucleotide sequence ID" value="XM_017487847.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SLG2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007065"/>
    <dbReference type="GeneID" id="108276291"/>
    <dbReference type="KEGG" id="ipu:108276291"/>
    <dbReference type="CTD" id="55741"/>
    <dbReference type="OrthoDB" id="8118055at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044322">
        <property type="term" value="C:endoplasmic reticulum quality control compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904380">
        <property type="term" value="P:endoplasmic reticulum mannose trimming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904154">
        <property type="term" value="P:positive regulation of retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000015">
        <property type="entry name" value="alpha-1,2-Mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044674">
        <property type="entry name" value="EDEM1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679:SF6">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0834">Unfolded protein response</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="364"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="385"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="487"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017343336.1"/>
        </source>
    </evidence>
    <sequence length="572" mass="64655" checksum="9964ACFBC45806FB" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SS10</accession>
    <name>A0A2D0SS10_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amyloid beta precursor protein binding family B member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">apbb1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017345281.1">
        <property type="nucleotide sequence ID" value="XM_017489792.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SS10"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003481"/>
    <dbReference type="GeneID" id="108277238"/>
    <dbReference type="KEGG" id="ipu:108277238"/>
    <dbReference type="CTD" id="322"/>
    <dbReference type="OMA" id="ELCNGEH"/>
    <dbReference type="OrthoDB" id="5969782at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001540">
        <property type="term" value="F:amyloid-beta binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01272">
        <property type="entry name" value="PTB1_Fe65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01271">
        <property type="entry name" value="PTB2_Fe65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000019">
        <property type="entry name" value="Amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000003">
        <property type="entry name" value="amyloid beta A4 precursor protein-binding family B member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000034">
        <property type="entry name" value="amyloid beta A4 precursor protein-binding family B member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039576">
        <property type="entry name" value="APBB1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14058">
        <property type="entry name" value="AMYLOID BETA A4 PRECURSOR PROTEIN-BINDING FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14058:SF5">
        <property type="entry name" value="AMYLOID BETA PRECURSOR PROTEIN BINDING FAMILY B MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="WW" evidence="5">
        <location>
            <begin position="291"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="PID" evidence="4">
        <location>
            <begin position="413"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="PID" evidence="4">
        <location>
            <begin position="582"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="127"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="184"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="313"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="127"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="200"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="260"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="333"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017345281.1"/>
        </source>
    </evidence>
    <sequence length="747" mass="81970" checksum="5FA2987B5ED0A523" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SSG2</accession>
    <name>A0A2D0SSG2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">D-ribitol-5-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="5">2.7.7.40</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase-like protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Isoprenoid synthase domain-containing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">crppa</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Cytidylyltransferase required for protein O-linked mannosylation. Catalyzes the formation of CDP-ribitol nucleotide sugar from D-ribitol 5-phosphate. CDP-ribitol is a substrate of FKTN during the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Shows activity toward other pentose phosphate sugars and mediates formation of CDP-ribulose or CDP-ribose using CTP and ribulose-5-phosphate or ribose-5-phosphate, respectively. Not involved in dolichol production.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>D-ribitol 5-phosphate + CTP + H(+) = CDP-L-ribitol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12456"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:57608"/>
            <dbReference type="ChEBI" id="CHEBI:57695"/>
            <dbReference type="EC" id="2.7.7.40"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>D-ribose 5-phosphate + CTP + H(+) = CDP-D-ribose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:53872"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:137525"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>D-ribulose 5-phosphate + CTP + H(+) = CDP-D-ribulose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:53612"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:137524"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.40" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017345632.1">
        <property type="nucleotide sequence ID" value="XM_017490143.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SSG2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034855"/>
    <dbReference type="GeneID" id="108277411"/>
    <dbReference type="KEGG" id="ipu:108277411"/>
    <dbReference type="CTD" id="729920"/>
    <dbReference type="OMA" id="LKEWNFI"/>
    <dbReference type="OrthoDB" id="414267at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047349">
        <property type="term" value="F:D-ribitol-5-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02516">
        <property type="entry name" value="CDP-ME_synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000080">
        <property type="entry name" value="D-ribitol-5-phosphate cytidylyltransferase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034683">
        <property type="entry name" value="IspD/TarI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040635">
        <property type="entry name" value="ISPD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018294">
        <property type="entry name" value="ISPD_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43015">
        <property type="entry name" value="D-RIBITOL-5-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43015:SF1">
        <property type="entry name" value="D-RIBITOL-5-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01128">
        <property type="entry name" value="IspD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18706">
        <property type="entry name" value="ISPD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01295">
        <property type="entry name" value="ISPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 18" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="D-ribitol-5-phosphate cytidylyltransferase C-terminal" evidence="16">
        <location>
            <begin position="248"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF18706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017345632.1"/>
        </source>
    </evidence>
    <sequence length="409" mass="45820" checksum="1F9533B5AC0B818F" modified="2017-12-20" version="1">MLGRDAVEFPVAVVLPAAGCGERTGLSTPKQFCTFLNRPLISFTIETFERIPWVESIVVVVAKESHELMLQIIHQFQHAKVKVVEGGTTRHRSIFNGLQAFSESAEGPVLPRPKVVIIHDAVRPFIEEDFLLKITLAAKEQGASGAIRPLVSTVIATTSEGFLDHSLERTKYRASEMPQGFLYDVIYQAYQRCSEFDFEFGTECLHLALQYSGTSAKLIEGPPTLWKVTYKRDLAAAESIIKETLLLSACLISGAMAETIELSNRLQKNLESNHMAVDVIPCTKENNKLLLKTRNFIQISTSGASLSETEEMVKFFKESKHALLYPVVIVWVHLRMSDEASDIQTNEELTAIMDLSMKVKQGNILLYCVQIIYSKVPELWERSVARLSEITESLVRDRNPVLTGQMLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0STM1</accession>
    <name>A0A2D0STM1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Coagulation factor X</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC108277713</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017346038.1">
        <property type="nucleotide sequence ID" value="XM_017490549.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0STM1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000002375"/>
    <dbReference type="GeneID" id="108277713"/>
    <dbReference type="KEGG" id="ipu:108277713"/>
    <dbReference type="OMA" id="VPYHNTW"/>
    <dbReference type="OrthoDB" id="5918597at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000012">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000120">
        <property type="entry name" value="Putative serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.740.10:FF:000001">
        <property type="entry name" value="vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.740.10">
        <property type="entry name" value="Coagulation Factor IX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017857">
        <property type="entry name" value="Coagulation_fac-like_Gla_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035972">
        <property type="entry name" value="GLA-like_dom_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000294">
        <property type="entry name" value="GLA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012224">
        <property type="entry name" value="Pept_S1A_FX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050442">
        <property type="entry name" value="Peptidase_S1_coag_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278">
        <property type="entry name" value="COAGULATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278:SF25">
        <property type="entry name" value="COAGULATION FACTOR IX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00594">
        <property type="entry name" value="Gla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001143">
        <property type="entry name" value="Factor_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00001">
        <property type="entry name" value="GLABLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00069">
        <property type="entry name" value="GLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57630">
        <property type="entry name" value="GLA-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00011">
        <property type="entry name" value="GLA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50998">
        <property type="entry name" value="GLA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 12" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 13" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 13" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012564936" evidence="14">
        <location>
            <begin position="18"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="Gla" evidence="17">
        <location>
            <begin position="39"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="85"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="16">
        <location>
            <begin position="237"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="11">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="11">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="11">
        <location>
            <position position="420"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001143-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017346038.1"/>
        </source>
    </evidence>
    <sequence length="490" mass="55149" checksum="17B835A111975214" modified="2017-12-20" version="1">MSDSVFCLVMFLFSTSAISLQSAVFIHHRDAVRILRHRRANYFLEEVKPGDLERECYEEICSKEEAAEIFQSREKTTEFWLRYQRQSQCRENVCFNGGVCTVSEGRYACVCMPRYTGKHCETEVFECEYKNGGCFHYCSDSQPVGSSVTCSCADGYVLEEDGKSCKVTAQFPCGKQWTRGILQRSFSDALNHTYTKENPTHYPTNNSLFTSNHTQSTENATHTLYNISTSDDSDTRIVGGLLQRQGGSPWQALLRRKDGNGFCGGTLIDKRWVITAAHCLQETPDHVTLGDFDKFRPDDGEQRIKVEKAIVHPHFHEYTFDSDIALLYLAEPVVFSSVVSPACMPNAHLAGRLERPGENGLVSGWGATHYLGRSSRFLLKVSLPVVDQKDCMDSTEQVITDNMFCAGFLHAEKDACSGDSGGPFVVNYRGTWFLTGVVSWGEQCAADGKYGVYTRISNFLYWIQDEIKKQEETISRNTKQAYTSTISSIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SUA9</accession>
    <name>A0A2D0SUA9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Sodium/glucose cotransporter 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Low affinity sodium-glucose cotransporter</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Solute carrier family 5 member 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">slc5a2</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Electrogenic Na(+)-coupled sugar symporter that actively transports D-glucose at the plasma membrane, with a Na(+) to sugar coupling ratio of 1:1. Transporter activity is driven by a transmembrane Na(+) electrochemical gradient set by the Na(+)/K(+) pump. Unlike SLC5A1/SGLT1, requires the auxiliary protein PDZK1IP1/MAP17 for full transporter activity. Has a primary role in D-glucose reabsorption from glomerular filtrate across the brush border of the early proximal tubules of the kidney.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>D-glucose(out) + Na(+)(out) = D-glucose(in) + Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:70571"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:70572"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="17">Forms a heterodimer (via TM13) with PDZK1IP1 (via N-terminal transmembrane helix); this interaction enhances SLC5A2 transporter activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 21">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017346277.1">
        <property type="nucleotide sequence ID" value="XM_017490788.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SUA9"/>
    <dbReference type="GeneID" id="108277829"/>
    <dbReference type="CTD" id="6524"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005412">
        <property type="term" value="F:D-glucose:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904659">
        <property type="term" value="P:D-glucose transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1730.10:FF:000010">
        <property type="entry name" value="Solute carrier family 5 member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018212">
        <property type="entry name" value="Na/solute_symporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11819:SF145">
        <property type="entry name" value="SODIUM_GLUCOSE COTRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11819">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00456">
        <property type="entry name" value="NA_SOLUT_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="14" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7" id="KW-0769">Symport</keyword>
    <keyword evidence="5 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="283"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="351"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="392"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="425"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="454"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="496"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="621"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00072719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00081561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_017346277.1"/>
        </source>
    </evidence>
    <sequence length="641" mass="70220" checksum="01477516C39C3CE2" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SYC6</accession>
    <name>A0A2D0SYC6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Lysosome membrane protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">scarb2b</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD36 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347672.1">
        <property type="nucleotide sequence ID" value="XM_017492183.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SYC6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000011975"/>
    <dbReference type="GeneID" id="108278698"/>
    <dbReference type="KEGG" id="ipu:108278698"/>
    <dbReference type="CTD" id="564077"/>
    <dbReference type="OMA" id="YYGISAV"/>
    <dbReference type="OrthoDB" id="18585at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006622">
        <property type="term" value="P:protein targeting to lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002159">
        <property type="entry name" value="CD36_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005429">
        <property type="entry name" value="LimpII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923:SF94">
        <property type="entry name" value="LYSOSOME MEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS B TYPE-1 SR-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01130">
        <property type="entry name" value="CD36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01609">
        <property type="entry name" value="CD36FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01611">
        <property type="entry name" value="LIMPII"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017347672.1"/>
        </source>
    </evidence>
    <sequence length="505" mass="56429" checksum="33F48D06CC9D0150" modified="2017-12-20" version="1">MMKCKVCLIYTSIALGVLMLIGGIGIILAGDLHARIENKLKGEITLTEGSKVFQSWKNPPPPVFMEFFFFNVTNPDGFMIGEKPRLTHMGPYTYREYRPKANVTFVENGAKVSALTPKTFVFVPEMSAGDPSVDLVTTVNIPAVAVMNKLKGVSFWVRSGIAMFMSSIQTTMFMTHTVDELLWGFKDPLLSRLRAAKPEVDENFGLMLYKNGSNDGEFVYYTGEKNYLDYGRIDTWKGEKLMSFWATNQSNMINGTDGSAFHPFLTKEERLNVFTADLCRSIHMFFEKEVVVKGIPAYRYTPPRAVLASGKNNPENEGFCLEKDKCLDDGVLNVAVCRKGAPVIVSFPHFYLADKKYVDAIEGISPVHEHHQTFLDLNPTTGVPVRAAKRAQINIKLDRVTGFPMTRNLNGTIFPIMFLNESVVIDDASAQKLQKLLLIVKIVPNFPYVLMVLGAVLSLIGLSLFFLSHRKKSSGKTEMSYTSVNRDDPVEKNGTYIGLTPVEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SYL9</accession>
    <name>A0A2D0SYL9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ankyrin repeat domain-containing protein SOWAHA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC108278667</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the SOWAH family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347626.1">
        <property type="nucleotide sequence ID" value="XM_017492137.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SYL9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000017313"/>
    <dbReference type="GeneID" id="108278667"/>
    <dbReference type="KEGG" id="ipu:108278667"/>
    <dbReference type="OMA" id="PPKPCML"/>
    <dbReference type="OrthoDB" id="432281at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058889">
        <property type="entry name" value="WHD_SOWAHA-C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14491:SF2">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN SOWAHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14491">
        <property type="entry name" value="SOSONDOWAH, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25877">
        <property type="entry name" value="WHD_SOWAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SOWAHA-C winged helix-turn-helix" evidence="6">
        <location>
            <begin position="2"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="358"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="79"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="166"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="230"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="423"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="94"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="423"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="464"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017347626.1"/>
        </source>
    </evidence>
    <sequence length="486" mass="53696" checksum="B39C94DA3D5437EF" modified="2017-12-20" version="1">MEITQESILSVLVEEGGKIQNSELVSKFKAPLNCSDPTEKKQNRDLFKMIINNVAVVKVTEEVKYVVLRKKYQHLLKNRSDDGTEEKEEGEGGEPEKVELNPAAELREVCENEAAPEENESEQKPPSDSGADPSHSSPAPSFIELALQRQKSTDFKVKKSLHFVTPLKSGTHDSQKHGPARSTNSETSEHKRFALPLRTPPVAVAPVAREDLSTMKIPSNDTNPVQVHLAPVSSTPGPPCSPRYKRRSSVDSVGISSSPQTRRHCKSAKPADEPKYSDTALLEISEHEWMVKSASGQWGYLYGLLLKDAQLAEKKDFISGFTALHWAAKCGNGDMLCKIIDLSRKNGNGVDINTKSYAGYTPLHVAAIHGQEYVIGILVNEYAANCNVRDNSGKKAHHYLDTGASRSIRELLGGLKVVRAEPQKNTEDTDTHKHAHTISRLFQPQTIGYKKKPKSRSSVLSTPEDVKDEKGEHSNPMHRVLSDVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T1I4</accession>
    <name>A0A2D0T1I4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8 9">Sphingomyelin phosphodiesterase 4 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7 8 9" type="primary">smpd4</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7 8 9" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7 8 9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017348774.1">
        <property type="nucleotide sequence ID" value="XM_017493285.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017348775.1">
        <property type="nucleotide sequence ID" value="XM_017493286.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017348776.1">
        <property type="nucleotide sequence ID" value="XM_017493287.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T1I4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000004715"/>
    <dbReference type="GeneID" id="108279222"/>
    <dbReference type="KEGG" id="ipu:108279222"/>
    <dbReference type="CTD" id="55627"/>
    <dbReference type="GeneTree" id="ENSGT00390000006044"/>
    <dbReference type="OrthoDB" id="10251508at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050290">
        <property type="term" value="F:sphingomyelin phosphodiesterase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006685">
        <property type="term" value="P:sphingomyelin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024129">
        <property type="entry name" value="Sphingomy_SMPD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12988">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12988:SF6">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14724">
        <property type="entry name" value="mit_SMPDase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="770"/>
            <end position="790"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017348774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017348775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017348776.1"/>
        </source>
    </evidence>
    <sequence length="820" mass="93290" checksum="C1081F4CE0260A70" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T900</accession>
    <name>A0A2D0T900_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Microtubule cross-linking factor 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC108280638</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017351331.2">
        <property type="nucleotide sequence ID" value="XM_017495842.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000025730"/>
    <dbReference type="GeneID" id="108280638"/>
    <dbReference type="KEGG" id="ipu:108280638"/>
    <dbReference type="OrthoDB" id="10036174at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049885">
        <property type="entry name" value="MTCL1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027882">
        <property type="entry name" value="SOGA1/2-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027881">
        <property type="entry name" value="SOGA_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15742">
        <property type="entry name" value="GIRDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15742:SF3">
        <property type="entry name" value="MICROTUBULE CROSS-LINKING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11365">
        <property type="entry name" value="SOGA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14818">
        <property type="entry name" value="SOGA1-2-like_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SOGA coiled-coil" evidence="7">
        <location>
            <begin position="423"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="SOGA coiled-coil" evidence="7">
        <location>
            <begin position="555"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="SOGA 1/2-like coiled-coil" evidence="8">
        <location>
            <begin position="1190"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="676"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="795"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="874"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1033"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1149"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1236"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1370"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1626"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1661"/>
            <end position="1719"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1739"/>
            <end position="1784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1834"/>
            <end position="1934"/>
        </location>
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    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="255"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="438"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="495"/>
            <end position="529"/>
        </location>
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    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="556"/>
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    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="629"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="55"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="85"/>
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    </feature>
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        <location>
            <begin position="149"/>
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        </location>
    </feature>
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    </feature>
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        <location>
            <begin position="230"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
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            <begin position="1048"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
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        </location>
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        <location>
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        <location>
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        </location>
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        <location>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017351331.2"/>
        </source>
    </evidence>
    <sequence length="1934" mass="214614" checksum="FF391902DA05A292" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0TB46</accession>
    <name>A0A2D0TB46_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Elongin-B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Elongin 18 kDa subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">RNA polymerase II transcription factor SIII subunit B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">SIII p18</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Transcription elongation factor B polypeptide 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">elob</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex). In embryonic stem cells, the elongin BC complex is recruited by EPOP to Polycomb group (PcG) target genes in order generate genomic region that display both active and repressive chromatin properties, an important feature of pluripotent stem cells.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Heterotrimer of an A (ELOA, ELOA2 or ELOA3P), ELOB and ELOC subunit. The elongin BC complex interacts with EPOP; leading to recruit the elongin BC complex to Polycomb group (PcG) target genes, thereby restricting excessive activity of the PRC2/EED-EZH2 complex. Component of multiple cullin-RING E3 ubiquitin-protein ligase complexes composed of Elongin BC (ELOB and ELOC), a cullin (either CUL2 or CUL5), a catalytic subunit (either RBX1 or RNF7/RBX2), as well as a substrate adapter protein that can be either ASB2, ASB9, ASB11, KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B, FEM1C, LRR1, PCMTD1, SOCS1, SOCS2, SOCS5, SPSB1, SPSB3, ELOA, VHL, WSB1 or RAB40C. As part of the Elongin BC E3 ubiquitin ligase complex; interacts with NRBP1. May also interact with DCUN1D1, DCUN1D2, DCUN1D3 and DCUN1D5. May form oligomers as a KLHDC2/KLHDC3-ELOB-ELOC complex; this interaction is autoinhibitory for the E3 ligase complex as the substrate-binding site of KLHDC2/KLHDC3 is blocked in the oligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the Elongin B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017351895.1">
        <property type="nucleotide sequence ID" value="XM_017496406.2"/>
    </dbReference>
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    <dbReference type="STRING" id="7998.ENSIPUP00000011792"/>
    <dbReference type="GeneID" id="108280902"/>
    <dbReference type="KEGG" id="ipu:108280902"/>
    <dbReference type="CTD" id="6923"/>
    <dbReference type="OrthoDB" id="7537057at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070449">
        <property type="term" value="C:elongin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030891">
        <property type="term" value="C:VCB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01788">
        <property type="entry name" value="Ubl_ElonginB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000108">
        <property type="entry name" value="Elongin-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039049">
        <property type="entry name" value="ELOB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13248:SF4">
        <property type="entry name" value="ELONGIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13248">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="18">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00074516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00083653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017351895.1"/>
        </source>
    </evidence>
    <sequence length="119" mass="13367" checksum="63160CE35748261D" modified="2017-12-20" version="1">MDVFLMIRRHKTTIFTDAKESTTVYELKRIVEGILKRAPEEQRLYKDDMMLEDSKTLGDCGFTNQTARPQAPATVGLAFRISDDAFEPLRVDAFSSPPELPDVMKPQDSGSTANEQAVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EZB8</accession>
    <name>A0A979EZB8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">POC1 centriolar protein homolog B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">poc1b</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the WD repeat POC1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047013248.1">
        <property type="nucleotide sequence ID" value="XM_047157292.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EZB8"/>
    <dbReference type="GeneID" id="108269393"/>
    <dbReference type="KEGG" id="ipu:108269393"/>
    <dbReference type="CTD" id="282809"/>
    <dbReference type="OrthoDB" id="10264588at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048871">
        <property type="term" value="P:multicellular organismal-level homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000235">
        <property type="entry name" value="POC1 centriolar protein homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050505">
        <property type="entry name" value="WDR55/POC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019:SF1">
        <property type="entry name" value="POC1 CENTRIOLAR PROTEIN HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="14"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="56"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="98"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="140"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="182"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="224"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="266"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="430"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_047013248.1"/>
        </source>
    </evidence>
    <sequence length="490" mass="54095" checksum="ACAB9C41933CAF15" modified="2023-02-22" version="1">MASVLEDPTLARHFKGHKDAVTCADFSPNNKQLATGSCDKTLMIWNLNPKSRAFRFVGHQDVVTAVHFSPSGELVASGSRDKTVRLWTPNIKGESMGFKAHTATVRSVSFSADGQRLVTASDDKSVKVWSVHRQKFIYSLNQHTNWVRCARFSPDGRLVASCGDDRTVRLWDTSTRLCVNTFTDYGGPATFVDFDSSGTCIASSGADNTLKIWDIRTNKLIQHYQVHSAAINTFSFHPSGNYLISGSSDSTVKIVDLLEGRLIYTLHGHKGPVLAVAFSREGDLFASGGSDSQVLMWKTNFDALNYREMLSRHRKRVTPDPPPHLTDIYPRSPHQHLAHIGAVEINPAVADVHSAHPHVVEIGEDPFYTTAAGRHTSGIREHPSTSAGLTSIFTHTQHQEEAREEENLGPGVTDLQEERAGTSPALRSTLEHIVQQLDILTQTVAVLEERLTLTEDKLRECLDNQAVILEQMQQQDESRKETGGAESPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F0T5</accession>
    <name>A0A979F0T5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Collagen alpha-6(VI) chain isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC108270894</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Collagen VI acts as a cell-binding protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the type VI collagen family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047013786.1">
        <property type="nucleotide sequence ID" value="XM_047157830.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108270894"/>
    <dbReference type="OrthoDB" id="6132182at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005589">
        <property type="term" value="C:collagen type VI trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01472">
        <property type="entry name" value="vWA_collagen"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd01450">
        <property type="entry name" value="vWFA_subfamily_ECM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000004">
        <property type="entry name" value="collagen alpha-6(VI) chain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000003">
        <property type="entry name" value="Collagen type VI alpha 3 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000021">
        <property type="entry name" value="Collagen, type VI, alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000001">
        <property type="entry name" value="Collagen, type XII, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.320">
        <property type="entry name" value="6-Phosphogluconate Dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050525">
        <property type="entry name" value="ECM_Assembly_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020:SF86">
        <property type="entry name" value="COLLAGEN, TYPE VI, ALPHA 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7 16" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5037041437" evidence="13">
        <location>
            <begin position="23"/>
            <end position="2695"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="14">
        <location>
            <begin position="34"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="14">
        <location>
            <begin position="236"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="14">
        <location>
            <begin position="431"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="14">
        <location>
            <begin position="646"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="14">
        <location>
            <begin position="851"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="14">
        <location>
            <begin position="1036"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="14">
        <location>
            <begin position="1233"/>
            <end position="1406"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="14">
        <location>
            <begin position="1424"/>
            <end position="1601"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="14">
        <location>
            <begin position="2185"/>
            <end position="2368"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="14">
        <location>
            <begin position="2395"/>
            <end position="2593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1826"/>
            <end position="1871"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1904"/>
            <end position="2156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="2673"/>
            <end position="2695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="1910"/>
            <end position="1919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1987"/>
            <end position="2000"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="2120"/>
            <end position="2129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="2673"/>
            <end position="2686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043858"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00044000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_047013786.1"/>
        </source>
    </evidence>
    <sequence length="2695" mass="294961" checksum="59EC2D9E48A3AC48" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F240</accession>
    <name>A0A979F240_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">elp4</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). The elongator complex catalyzes the formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ELP4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047014234.1">
        <property type="nucleotide sequence ID" value="XM_047158278.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979F240"/>
    <dbReference type="GeneID" id="108271096"/>
    <dbReference type="CTD" id="26610"/>
    <dbReference type="OrthoDB" id="289162at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19494">
        <property type="entry name" value="Elp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002074">
        <property type="entry name" value="Elongator acetyltransferase complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008728">
        <property type="entry name" value="Elongator_complex_protein_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12896:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12896">
        <property type="entry name" value="PAX6 NEIGHBOR PROTEIN PAXNEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05625">
        <property type="entry name" value="PAXNEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="108"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_047014234.1"/>
        </source>
    </evidence>
    <sequence length="396" mass="43552" checksum="BAAB5275F63F6B50" modified="2023-02-22" version="1">MAAPMENATKGTRLSGSSTSFQKKSRSKLIQIPGTKPSVHTGQLIVSSGVPSLDCALGGGLAVGSLLLIEEDEFDSYSHALLQYFLAEGVMSGHELFVASAQDHPDDIIKDLPSPLQDEDPDLKNASSSSPGQQDAMKIAWRYQNLPKFQSVLSSSSRFGHYYDLSKHMEQDLLQHAKIHTFYLPEEQPASTHLSGVLGPYSVLLHSIQSLIRQNGFDGSNSQVKTCNVLRLALHSLGSGLWGDDVCVSPAHNHALSTFLYVLRGLLRSSLSVAMVTVPTHLIRNKLLRGRLIRLSDTAIALQSFSAADRNNSLYKDYHGLLHVIQVPRLNCLGCEVPDTKDLAFRLKRKQFTIERMYLPPDLSDSVSRGKQHLSHWSSFCLTASSEWSRSSRAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979FCK6</accession>
    <name>A0A979FCK6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pleckstrin homology-like domain family B member 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Protein LL5-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">phldb1b</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
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    </reference>
    <reference evidence="10 11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11">Blood</tissue>
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        <property type="nucleotide sequence ID" value="XM_047161523.2"/>
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        <property type="nucleotide sequence ID" value="XM_047161524.2"/>
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    <dbReference type="GeneID" id="108277892"/>
    <dbReference type="KEGG" id="ipu:108277892"/>
    <dbReference type="CTD" id="570275"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
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    <dbReference type="GO" id="GO:0045180">
        <property type="term" value="C:basal cortex"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0070507">
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="FHA_PHLB1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PH_PHLDB1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pleckstrin homology like domain family B member 1"/>
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        <property type="entry name" value="pleckstrin homology-like domain family B member 1 isoform X1"/>
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    </dbReference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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        <property type="entry name" value="AH/BAR_dom_sf"/>
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        <property type="entry name" value="FHA_dom"/>
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    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
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        <property type="entry name" value="PH-like_domain"/>
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    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
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        <property type="entry name" value="PHLDB1/2/3_PH"/>
    </dbReference>
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        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PLECKSTRIN HOMOLOGY-LIKE DOMAIN FAMILY B MEMBER 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12156">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY-LIKE DOMAIN, FAMILY B, MEMBER 3"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="BAR/IMD domain-like"/>
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    </dbReference>
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        <property type="entry name" value="SMAD/FHA domain"/>
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    </dbReference>
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        <location>
            <begin position="1416"/>
            <end position="1529"/>
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    <feature type="region of interest" description="Disordered" evidence="7">
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            <begin position="1122"/>
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            <begin position="736"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="7">
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00069090"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00077655"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_047017479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_047017480.1"/>
        </source>
    </evidence>
    <sequence length="1536" mass="171302" checksum="9F75F622B11B0CFF" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979FD35</accession>
    <name>A0A979FD35_ICTPU</name>
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        <recommendedName>
            <fullName evidence="14">Flap endonuclease GEN homolog 1</fullName>
        </recommendedName>
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        <name evidence="19" type="primary">LOC108280062</name>
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    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
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    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
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    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="13">Largely monomeric, dimerizes on the Holliday junction and the first nick occurs upon dimerization at the junction.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the XPG/RAD2 endonuclease family. GEN subfamily.</text>
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    <dbReference type="AlphaFoldDB" id="A0A979FD35"/>
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        <property type="component" value="Chromosome 19"/>
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    <dbReference type="GO" id="GO:0005634">
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0017108">
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09869">
        <property type="entry name" value="PIN_GEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="flap endonuclease GEN homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Flap endonuclease GEN-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="5-3_exonuclease_C_sf"/>
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    <dbReference type="InterPro" id="IPR041012">
        <property type="entry name" value="GEN_chromo"/>
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    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF70">
        <property type="entry name" value="FLAP ENDONUCLEASE GEN HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18704">
        <property type="entry name" value="Chromo_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 19" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="17">
        <location>
            <begin position="56"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="16">
        <location>
            <begin position="183"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="662"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="724"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="749"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="884"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="662"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="805"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="839"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="888"/>
            <end position="919"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00063132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00070188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_047018126.1"/>
        </source>
    </evidence>
    <sequence length="948" mass="106158" checksum="937899B6306EA80F" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QTM9</accession>
    <name>A0A9F7QTM9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC108280614</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108280614</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053529660.1">
        <property type="nucleotide sequence ID" value="XM_053673685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QTM9"/>
    <dbReference type="GeneID" id="108280614"/>
    <dbReference type="KEGG" id="ipu:108280614"/>
    <dbReference type="OrthoDB" id="10580477at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053529660.1"/>
        </source>
    </evidence>
    <sequence length="141" mass="16024" checksum="7F6F1066CF17AB8E" modified="2023-06-28" version="1">MCVYSEHETLIYWYLVAVNLLIVILVIIVCVLRRKYTENQVTTRDRSNDTTVHTAESEGSYSSIAFKSRKKKSEQRNLEQRTFTAEDSVTYSSVAVSKPKSSASTDNEQDVNYSFIKIVSKNKTKSPKDVGDETIYSSMVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R033</accession>
    <name>A0A9F7R033_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">E3 ubiquitin-protein ligase TRIM35</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC108262627</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053531120.1">
        <property type="nucleotide sequence ID" value="XM_053675145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R033"/>
    <dbReference type="GeneID" id="108262627"/>
    <dbReference type="KEGG" id="ipu:108262627"/>
    <dbReference type="OrthoDB" id="6105938at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12893">
        <property type="entry name" value="SPRY_PRY_TRIM35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="12"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="84"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="8">
        <location>
            <begin position="272"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="194"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053531120.1"/>
        </source>
    </evidence>
    <sequence length="470" mass="54224" checksum="EE9969F50A792DA2" modified="2023-06-28" version="1">MASKFSEEDFSCPVCCEIFTDPVLLRCSHSVCKVCLQKFWETKGSRECPVCRRKSSMEDPPTNLALKNLCETFLQERSQRSSSASETVCSLHSEKLKLFCLDDQQPVCVVCRDSKIHTNHKFCPIDEALTDCKEELKTALKPLQEKLKIFKDCKLHWSQTAEHIKIQAQHTERQIKEEFEKLHQVLRDEEAARIAALREEEEQKSQMMKEKIEKLSRDMSSLSDTIRGVEEEMRAEDVSFLQNYKATVRRAQCTLQHPEELSGPLIHVAKHLDNLKFRVWEKMQDAVQYTPVTLDPNTAHPKLIVSDNLTSVRFSDEKQKLPDNPERFDERCCILGSEVFNSGTHCWDVEVGDCTRWFVGVMTESAQRKGNIFSRSGIWYLWYFVGKYGTCSTPQPDALLSVTQNLQRIRVQLDWNRGKLSFSDPLTNTHIHTFTHTFTDKLLPFIRVGCNKSPVKILPLQCSVRVNQIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R2G0</accession>
    <name>A0A9F7R2G0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chloride channel protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">clcn2c</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>thiocyanate(in) = thiocyanate(out)</text>
            <dbReference type="Rhea" id="RHEA:75347"/>
            <dbReference type="ChEBI" id="CHEBI:18022"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="22">Homodimer. Interacts with auxiliary subunit HEPACAM.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Basolateral cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendritic spine membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the chloride channel (TC 2.A.49) family. ClC-2/CLCN2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053535179.1">
        <property type="nucleotide sequence ID" value="XM_053679204.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R2G0"/>
    <dbReference type="GeneID" id="108277947"/>
    <dbReference type="KEGG" id="ipu:108277947"/>
    <dbReference type="CTD" id="563453"/>
    <dbReference type="OrthoDB" id="4564at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032591">
        <property type="term" value="C:dendritic spine membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03683">
        <property type="entry name" value="ClC_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3080.10:FF:000002">
        <property type="entry name" value="Chloride channel 2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000056">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000019">
        <property type="entry name" value="Chloride voltage-gated channel 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002244">
        <property type="entry name" value="Cl-channel-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050970">
        <property type="entry name" value="Cl_channel_volt-gated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720:SF6">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01113">
        <property type="entry name" value="CLCHANNEL2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="20" id="KW-0966">Cell projection</keyword>
    <keyword evidence="18" id="KW-0868">Chloride</keyword>
    <keyword evidence="17" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0770">Synapse</keyword>
    <keyword evidence="9 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="11" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="86"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="234"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="422"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="447"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="485"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="513"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="646"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="853"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="646"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="675"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="853"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="864"/>
            <end position="873"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00036895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00046437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_053535179.1"/>
        </source>
    </evidence>
    <sequence length="873" mass="97262" checksum="8CFB771D50F284F3" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R6I7</accession>
    <name>A0A9F7R6I7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="1">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19" type="primary">LOC108271691</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4 5" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3 4 5">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="1"/>
    <dbReference type="RefSeq" id="XP_053534960.1">
        <property type="nucleotide sequence ID" value="XM_053678985.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534961.1">
        <property type="nucleotide sequence ID" value="XM_053678986.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534962.1">
        <property type="nucleotide sequence ID" value="XM_053678987.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534963.1">
        <property type="nucleotide sequence ID" value="XM_053678988.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534964.1">
        <property type="nucleotide sequence ID" value="XM_053678989.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534965.1">
        <property type="nucleotide sequence ID" value="XM_053678990.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534966.1">
        <property type="nucleotide sequence ID" value="XM_053678991.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534967.1">
        <property type="nucleotide sequence ID" value="XM_053678992.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534968.1">
        <property type="nucleotide sequence ID" value="XM_053678993.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534969.1">
        <property type="nucleotide sequence ID" value="XM_053678994.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534970.1">
        <property type="nucleotide sequence ID" value="XM_053678995.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534971.1">
        <property type="nucleotide sequence ID" value="XM_053678996.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534972.1">
        <property type="nucleotide sequence ID" value="XM_053678997.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534974.1">
        <property type="nucleotide sequence ID" value="XM_053678999.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534975.1">
        <property type="nucleotide sequence ID" value="XM_053679000.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534976.1">
        <property type="nucleotide sequence ID" value="XM_053679001.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534977.1">
        <property type="nucleotide sequence ID" value="XM_053679002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R6I7"/>
    <dbReference type="GeneID" id="108271691"/>
    <dbReference type="KEGG" id="ipu:108271691"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF28">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053534960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053534961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053534962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_053534963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053534964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053534965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_053534966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053534967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053534968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053534969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_053534970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_053534971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_053534972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_053534974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_053534975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_053534976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_053534977.1"/>
        </source>
    </evidence>
    <sequence length="271" mass="30477" checksum="9252BCE38F04EABF" modified="2023-06-28" version="1">MNLFWIQAEEAQRKLKRQNGEDPALPPFCPLFASLGNILQCDVLLGMLGAVLQWAMEPTGGHWSESMLQRMVVSIKTKRECTTAAPPCEGTGHCHKETVWDKDKAERKRKAELCWKKTMAQTLESQSHFINKYIDLLTQDSEDLDASASTSAEHSPSSCDGALVSVGPRHWRARGGERRQMEMCVLCHEVQEILPDGTAMVLAAFVQRSTVMSKNRKRPPHNPESYDPLFMHPDLSFGTHTDSCGHIMHSHCWQRIYNHATDKITGLHSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R6U5</accession>
    <name>A0A9F7R6U5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC128629974</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein LOC128632514</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC128629974</name>
        <name evidence="4" type="synonym">LOC128632514</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3 4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053534502.1">
        <property type="nucleotide sequence ID" value="XM_053678527.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053535355.1">
        <property type="nucleotide sequence ID" value="XM_053679380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R6U5"/>
    <dbReference type="GeneID" id="128629974"/>
    <dbReference type="KEGG" id="ipu:128629974"/>
    <dbReference type="KEGG" id="ipu:128632514"/>
    <dbReference type="OrthoDB" id="8446581at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053534502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053535355.1"/>
        </source>
    </evidence>
    <sequence length="353" mass="39613" checksum="11C05E3BBF43A786" modified="2023-06-28" version="1">MTVENQTARDSSFQSSKMSRRRACPVCRRFYTNVQAHLAQIHQQGPEERGLHLAAASGRKGFRRLDCHLFDLHQLAGDTVRKLLLGAKIKLLNNNCKLSASEMSPKPRPTASRRGDTSSTSFTLWANTDSPLAAHLKYIDNRLTEGRVGSTRLSSLQRLLQWECRHLSGRVVLHRQGVRAEKSVGVLTAPARQTVGRCPCFADLKARPADPDMLNHCIGLLCAHFFSVSGHRTGVLQNLTTEEFENLEKADGGLFLINVKQHKPASAFGLAKLAVTKLELKWLKAFCRLRKHMAGYETSVGWLFFSGTQARISAHVAQPKFPLFFFFFLSTWTELRAEGEESVQTRELQTDHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R7H0</accession>
    <name>A0A9F7R7H0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transmembrane protein 14A isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tmem14a</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053535425.1">
        <property type="nucleotide sequence ID" value="XM_053679450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R7H0"/>
    <dbReference type="GeneID" id="108263132"/>
    <dbReference type="KEGG" id="ipu:108263132"/>
    <dbReference type="CTD" id="28978"/>
    <dbReference type="OrthoDB" id="5620at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070453">
        <property type="term" value="P:regulation of heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1740">
        <property type="entry name" value="Transmembrane protein 14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005349">
        <property type="entry name" value="TMEM14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044890">
        <property type="entry name" value="TMEM14_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12668">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 14, 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12668:SF11">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 14A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03647">
        <property type="entry name" value="Tmemb_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053535425.1"/>
        </source>
    </evidence>
    <sequence length="110" mass="11893" checksum="70F81F2A89A0C7C8" modified="2023-06-28" version="1">MAVDWLGFVYAATILFGGFMGYKRKGSLVSLIAGLFFGGISTYGAFRITMDPQDKWTSLTASGVLAVVMGIRFKNSGKLMPAGIMAGLSCFVLTFNPHDCNSHFKFHSVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R874</accession>
    <name>A0A9F7R874_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Zinc finger protein 585A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC108268650</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053538329.1">
        <property type="nucleotide sequence ID" value="XM_053682354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R874"/>
    <dbReference type="GeneID" id="108268650"/>
    <dbReference type="KEGG" id="ipu:108268650"/>
    <dbReference type="OrthoDB" id="9439903at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19193">
        <property type="entry name" value="PR-SET_PRDM7_9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001818">
        <property type="entry name" value="GDNF-inducible zinc finger protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002063">
        <property type="entry name" value="RB associated KRAB zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002005">
        <property type="entry name" value="Zinc finger protein 200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002175">
        <property type="entry name" value="Zinc finger protein 234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000060">
        <property type="entry name" value="zinc finger protein 436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001270">
        <property type="entry name" value="zinc finger protein 583 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000051">
        <property type="entry name" value="zinc finger protein 585A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger protein 672"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044417">
        <property type="entry name" value="PRDM7_9_PR-SET"/>
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    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
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    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
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    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
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    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
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    <dbReference type="PANTHER" id="PTHR23226:SF416">
        <property type="entry name" value="FI01424P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21549">
        <property type="entry name" value="PRDM2_PR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
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    </dbReference>
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    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="124"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="268"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="321"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="349"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="377"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="405"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="433"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="461"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="18">
        <location>
            <begin position="661"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="810"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="863"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="891"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="919"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="947"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="975"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="1003"/>
            <end position="1030"/>
        </location>
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    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="1031"/>
            <end position="1058"/>
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    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
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    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="1087"/>
            <end position="1114"/>
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    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="1115"/>
            <end position="1139"/>
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            <begin position="1"/>
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    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="562"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
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            <begin position="26"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
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    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="572"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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    </evidence>
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        <source>
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    <evidence type="ECO:0000256" key="6">
        <source>
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        <source>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
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    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
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    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_053538329.1"/>
        </source>
    </evidence>
    <sequence length="1139" mass="131104" checksum="41D847EB162F2E17" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R8M7</accession>
    <name>A0A9F7R8M7_ICTPU</name>
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        <recommendedName>
            <fullName evidence="1">[histone H3]-lysine(4) N-trimethyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.354</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">smyd3</name>
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    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11">SDA103</strain>
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    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
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        </reaction>
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        <property type="nucleotide sequence ID" value="XM_053682564.1"/>
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    <dbReference type="OrthoDB" id="265717at2759"/>
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        <property type="component" value="Chromosome 9"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:histone H3K4 trimethyltransferase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="TPR-like_helical_dom_sf"/>
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        <property type="entry name" value="Znf_MYND"/>
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        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197:SF288">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SMYD3"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
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    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="9">
        <location>
            <begin position="12"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="MYND-type" evidence="10">
        <location>
            <begin position="57"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053538539.1"/>
        </source>
    </evidence>
    <sequence length="431" mass="48980" checksum="D50C8FF3EC7826C4" modified="2023-06-28" version="1">MAVSSVRCKVESKMERFISPGKGNGLRALSEIKPGQLIYSSEPIAFCVAEKFLKSTCQTCLCRGESLLRCSQCKSSRYCSVQCQKEAWPEHKRECKCLQHVYPRVPTDSVRLTARIIFTLLSDPAADSQELYSITEHQSHLEEMSEEKREGLVQLCSMLKLYLDSDVSQLPSGLDPMSLLARVMCNCFSVSDGELQDVGLGLYPSMSLLNHDCRPNCVMMFVRKKLKLRAVRRIQPFEELCISYTDILSPCVERRTQLMDQFHFLCQCQRCSDDQSDCTMLGGEESRWTIIRDSLPHLETLRHGEKWVELQQACQALIGAHSAAVPDSNVFMLRVLDSLMDSCITLSQYETALQYGTRTLTAYLLHYPDPHPSHAVELLRVAKLQHYSGDVEQAERTFTRAYDVMKVTHGSDHPLVTEVCRKLAECQAERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R9X0</accession>
    <name>A0A9F7R9X0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Two pore segment channel 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tpcn3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053532663.1">
        <property type="nucleotide sequence ID" value="XM_053676688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R9X0"/>
    <dbReference type="GeneID" id="108259497"/>
    <dbReference type="KEGG" id="ipu:108259497"/>
    <dbReference type="CTD" id="557460"/>
    <dbReference type="OrthoDB" id="416585at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46726">
        <property type="entry name" value="TWO PORE CHANNEL 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46726:SF1">
        <property type="entry name" value="TWO-PORE CALCIUM CHANNEL 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="255"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="410"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="440"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="468"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="526"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="634"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="6">
        <location>
            <begin position="67"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="6">
        <location>
            <begin position="409"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053532663.1"/>
        </source>
    </evidence>
    <sequence length="766" mass="87999" checksum="1603582A8AEDFA5B" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RBQ1</accession>
    <name>A0A9F7RBQ1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="34">Fatty-acid amide hydrolase 1</fullName>
            <ecNumber evidence="3">3.5.1.99</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="37">Anandamide amidohydrolase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="35">Fatty acid ester hydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="36">Oleamide hydrolase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="42" type="primary">LOC128634042</name>
    </gene>
    <organism evidence="41 42">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="41" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="41">SDA103</strain>
        </source>
    </reference>
    <reference evidence="42" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="42">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(11Z)-eicosenamide + H2O = (11Z)-eicosenoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:63120"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:32426"/>
            <dbReference type="ChEBI" id="CHEBI:146167"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:63121"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(11Z,14Z)-eicosadienamide + H2O = (11Z,14Z)-eicosadienoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:63004"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:77220"/>
            <dbReference type="ChEBI" id="CHEBI:146165"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:63005"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>(11Z,14Z,17Z)-eicosatrienamide + H2O = (11Z,14Z,17Z)-eicosatrienoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:63000"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:77223"/>
            <dbReference type="ChEBI" id="CHEBI:146164"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:63001"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>(15Z)-tetracosenamide + H2O = (15Z)-tetracosenoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:63028"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:32392"/>
            <dbReference type="ChEBI" id="CHEBI:146166"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:63029"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenamide + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:63016"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:137830"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:63017"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>(6Z)-octadecenamide + H2O = (6Z)-octadecenoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:63008"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:32375"/>
            <dbReference type="ChEBI" id="CHEBI:146168"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:63009"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>(8Z,11Z,14Z)-eicosatrienamide + H2O = (8Z,11Z,14Z)-eicosatrienoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:62996"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:71589"/>
            <dbReference type="ChEBI" id="CHEBI:146163"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:62997"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(9Z)-octadecenamide + H2O = (9Z)-octadecenoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:26506"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:116314"/>
            <dbReference type="EC" id="3.5.1.99"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:26507"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(9Z)-octadecenoate + glycine = N-(9Z-octadecenoyl)glycine + H2O</text>
            <dbReference type="Rhea" id="RHEA:51316"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:133992"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="8">
            <dbReference type="Rhea" id="RHEA:51318"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>(9Z,12Z)-octadecadienamide + H2O = (9Z,12Z)-octadecadienoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:63020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:82984"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:63021"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>(9Z,12Z,15Z)-octadecatrienamide + H2O = (9Z,12Z,15Z)-octadecatrienoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:62976"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:32387"/>
            <dbReference type="ChEBI" id="CHEBI:142684"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:62977"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1-O-methyl-(5Z,8Z,11Z,14Z)-eicosatetraenoate + H2O = methanol + (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63052"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:78033"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:63053"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + H2O = glycerol + (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:26132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:52392"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:26133"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N-(15Z-tetracosenoyl)-ethanolamine + H2O = (15Z)-tetracosenoate + ethanolamine</text>
            <dbReference type="Rhea" id="RHEA:63144"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32392"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="ChEBI" id="CHEBI:146187"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:63145"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>N-(15Z-tetracosenoyl)-taurine + H2O = (15Z)-tetracosenoate + taurine</text>
            <dbReference type="Rhea" id="RHEA:63160"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32392"/>
            <dbReference type="ChEBI" id="CHEBI:146198"/>
            <dbReference type="ChEBI" id="CHEBI:507393"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:63161"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-glycine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycine</text>
            <dbReference type="Rhea" id="RHEA:64108"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:59002"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:64109"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-L-serine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:64116"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:149697"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:64117"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine + H2O = ethanolamine + (5Z,8Z,11Z,14Z)-eicosatetraenoate</text>
            <dbReference type="Rhea" id="RHEA:26136"/>
            <dbReference type="ChEBI" id="CHEBI:2700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="EC" id="3.5.1.99"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:26137"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>N-(9Z-hexadecenoyl) ethanolamine + H2O = (9Z)-hexadecenoate + ethanolamine</text>
            <dbReference type="Rhea" id="RHEA:35563"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32372"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="ChEBI" id="CHEBI:71465"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:35564"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-(9Z-octadecenoyl) ethanolamine + H2O = ethanolamine + (9Z)-octadecenoate</text>
            <dbReference type="Rhea" id="RHEA:45060"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="ChEBI" id="CHEBI:71466"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:45061"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>N-(9Z-octadecenoyl)-taurine + H2O = taurine + (9Z)-octadecenoate</text>
            <dbReference type="Rhea" id="RHEA:63148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:146191"/>
            <dbReference type="ChEBI" id="CHEBI:507393"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:63149"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>N-docosanoyl-ethanolamine + H2O = docosanoate + ethanolamine</text>
            <dbReference type="Rhea" id="RHEA:63128"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:23858"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="ChEBI" id="CHEBI:146186"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:63129"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>N-docosanoyl-taurine + H2O = docosanoate + taurine</text>
            <dbReference type="Rhea" id="RHEA:63156"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:23858"/>
            <dbReference type="ChEBI" id="CHEBI:146196"/>
            <dbReference type="ChEBI" id="CHEBI:507393"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:63157"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>N-octadecanoyl ethanolamine + H2O = octadecanoate + ethanolamine</text>
            <dbReference type="Rhea" id="RHEA:63124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="ChEBI" id="CHEBI:85299"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:63125"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>N-tetracosanoyl-taurine + H2O = tetracosanoate + taurine</text>
            <dbReference type="Rhea" id="RHEA:63140"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:31014"/>
            <dbReference type="ChEBI" id="CHEBI:132049"/>
            <dbReference type="ChEBI" id="CHEBI:507393"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:63141"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>N-tricosanoyl-taurine + H2O = tricosanoate + taurine</text>
            <dbReference type="Rhea" id="RHEA:63164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:79007"/>
            <dbReference type="ChEBI" id="CHEBI:146197"/>
            <dbReference type="ChEBI" id="CHEBI:507393"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:63165"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>tetradecamide + H2O = tetradecanoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:62992"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30807"/>
            <dbReference type="ChEBI" id="CHEBI:137125"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:62993"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amidase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.99" evidence="3"/>
    <dbReference type="RefSeq" id="XP_053539830.1">
        <property type="nucleotide sequence ID" value="XM_053683855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RBQ1"/>
    <dbReference type="GeneID" id="128634042"/>
    <dbReference type="KEGG" id="ipu:128634042"/>
    <dbReference type="OrthoDB" id="6428749at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017064">
        <property type="term" value="F:fatty acid amide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1300.10:FF:000001">
        <property type="entry name" value="Fatty-acid amide hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052096">
        <property type="entry name" value="Endocannabinoid_amidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45847">
        <property type="entry name" value="FATTY ACID AMIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45847:SF6">
        <property type="entry name" value="FATTY ACID AMIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="39" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="41" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="39" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="39" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="39">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Amidase" evidence="40">
        <location>
            <begin position="90"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="38">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="38">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="38">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00051346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00051454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00051492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00051914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00052289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00052337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00052426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00052458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00052512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00052514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00052634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00052709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00052818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00052857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00052906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00073178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00077111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00077157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00077216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="40">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="41">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="42">
        <source>
            <dbReference type="RefSeq" id="XP_053539830.1"/>
        </source>
    </evidence>
    <sequence length="579" mass="63485" checksum="DBF60A4E0E0FA8F6" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RFG9</accession>
    <name>A0A9F7RFG9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein LOC108262313</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC108262313</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053541855.1">
        <property type="nucleotide sequence ID" value="XM_053685880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RFG9"/>
    <dbReference type="GeneID" id="108262313"/>
    <dbReference type="KEGG" id="ipu:108262313"/>
    <dbReference type="OrthoDB" id="8871851at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF111">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039913431" evidence="9">
        <location>
            <begin position="28"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="155"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="19"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053541855.1"/>
        </source>
    </evidence>
    <sequence length="213" mass="23654" checksum="4A0B682D7A55884D" modified="2023-06-28" version="1">MAKLLSTFLLQMLFGFFTPCLLEIASTDHSSVPPGENITLLCNITNYSEISWYRLRSEEVKLLISAEELKLEKTVFLRYNVNKSHFDITESSSSVSLVIIGVRETDLGFYFCGGRNNATHIQFGKLIRLDFTADQHHETDRSDTQQSVGPALCGIIIIILLCVCAVFVSISIICSSLYCSTLQDKCSSTGDSNEKEVDLHLSDLTSVTYTSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RG78</accession>
    <name>A0A9F7RG78_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">[histone H3]-trimethyl-L-lysine(4) demethylase</fullName>
            <ecNumber evidence="4">1.14.11.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">kdm5c</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + 3 2-oxoglutarate + 3 O2 = L-lysyl(4)-[histone H3] + 3 formaldehyde + 3 succinate + 3 CO2</text>
            <dbReference type="Rhea" id="RHEA:60208"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="1.14.11.67"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the JARID1 histone demethylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.67" evidence="4"/>
    <dbReference type="RefSeq" id="XP_053536111.1">
        <property type="nucleotide sequence ID" value="XM_053680136.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108265475"/>
    <dbReference type="CTD" id="8242"/>
    <dbReference type="OrthoDB" id="1678912at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034647">
        <property type="term" value="F:histone H3K4me/H3K4me2/H3K4me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16875">
        <property type="entry name" value="ARID_KDM5C_5D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15604">
        <property type="entry name" value="PHD1_KDM5C_5D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15608">
        <property type="entry name" value="PHD2_KDM5C_5D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000035">
        <property type="entry name" value="PHD transcription factor Rum1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.60:FF:000001">
        <property type="entry name" value="Putative lysine-specific demethylase 5b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000001">
        <property type="entry name" value="Putative lysine-specific demethylase 5b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048615">
        <property type="entry name" value="KDM5_C-hel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013637">
        <property type="entry name" value="Lys_sp_deMease-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004198">
        <property type="entry name" value="Znf_C5HC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF43">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21323">
        <property type="entry name" value="KDM5_C-hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08429">
        <property type="entry name" value="PLU-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02928">
        <property type="entry name" value="zf-C5HC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="10" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjN" evidence="19">
        <location>
            <begin position="15"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="ARID" evidence="18">
        <location>
            <begin position="80"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="17">
        <location>
            <begin position="363"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="20">
        <location>
            <begin position="507"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="174"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1221"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1382"/>
            <end position="1415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1424"/>
            <end position="1443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1524"/>
            <end position="1578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="198"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="229"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1239"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1382"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1424"/>
            <end position="1433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="1532"/>
            <end position="1545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1546"/>
            <end position="1562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1569"/>
            <end position="1578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_053536111.1"/>
        </source>
    </evidence>
    <sequence length="1578" mass="178576" checksum="DA45D6625AB7DDD6" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RGY2</accession>
    <name>A0A9F7RGY2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fucose-1-phosphate guanylyltransferase isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">fpgt</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053536516.1">
        <property type="nucleotide sequence ID" value="XM_053680541.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RGY2"/>
    <dbReference type="GeneID" id="108266010"/>
    <dbReference type="KEGG" id="ipu:108266010"/>
    <dbReference type="CTD" id="8790"/>
    <dbReference type="OMA" id="DMIAYRE"/>
    <dbReference type="OrthoDB" id="10062280at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042350">
        <property type="term" value="P:GDP-L-fucose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012887">
        <property type="entry name" value="GDP_fucose_pyrophosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15045">
        <property type="entry name" value="FUCOSE-1-PHOSPHATE GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15045:SF1">
        <property type="entry name" value="FUCOSE-1-PHOSPHATE GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07959">
        <property type="entry name" value="Fucose_pyrophosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GDP-fucose pyrophosphorylase" evidence="3">
        <location>
            <begin position="105"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053536516.1"/>
        </source>
    </evidence>
    <sequence length="574" mass="64641" checksum="262C64578329F60C" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RJY0</accession>
    <name>A0A9F7RJY0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Titin</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">Connectin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="primary">ttn.2</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="31">SDA103</strain>
        </source>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="32">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="24">Key component in the assembly and functioning of vertebrate striated muscles. By providing connections at the level of individual microfilaments, it contributes to the fine balance of forces between the two halves of the sarcomere. The size and extensibility of the cross-links are the main determinants of sarcomere extensibility properties of muscle. In non-muscle cells, seems to play a role in chromosome condensation and chromosome segregation during mitosis. Might link the lamina network to chromatin or nuclear actin, or both during interphase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="RefSeq" id="XP_053536757.1">
        <property type="nucleotide sequence ID" value="XM_053680782.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108266288"/>
    <dbReference type="KEGG" id="ipu:108266288"/>
    <dbReference type="CTD" id="317731"/>
    <dbReference type="OrthoDB" id="5969272at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00054857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00073138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00075299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="RefSeq" id="XP_053536757.1"/>
        </source>
    </evidence>
    <sequence length="30027" mass="3336451" checksum="CCFFEEB35024A095" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RK27</accession>
    <name>A0A9F7RK27_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein LOC108268727</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC108268727</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053538271.1">
        <property type="nucleotide sequence ID" value="XM_053682296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RK27"/>
    <dbReference type="GeneID" id="108268727"/>
    <dbReference type="KEGG" id="ipu:108268727"/>
    <dbReference type="OrthoDB" id="8947657at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF133">
        <property type="entry name" value="IMMUNE-TYPE RECEPTOR 5 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039900320" evidence="9">
        <location>
            <begin position="28"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="260"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="45"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="146"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053538271.1"/>
        </source>
    </evidence>
    <sequence length="351" mass="39418" checksum="9BFDA2D7AC75B25C" modified="2023-06-28" version="1">MLYTDLRLKIMMLMWITAMLFNKIADSTQSEGVNQPNTMITADVGENVTLHCFRLGEENTHSIVWYKQKVGHEPHVMVSVQHEPSYEKEFNPPKFSIEKEIKEKGFHLKIANVEPSDEAVYYCGYRKFFIRFGKGTFLAVKGDGDVKVSVVQSGVSDSVPAGASVTLQCSVLSESRSAELQVLWFRAAPPQSHPQIIYTHDNSSHQCESGSSTHTCVYNFSKNILSLSDTGTYYCAVAVCGKIIFGNGTRVQLERSGDPVVIYLAVALGVCVVVIFALIFVITCGWRHFEKRRVRCIQGSVTEKTLNQDCDNVELNYVALHFNERRAKRGRVKKEQPQDIIYSDVRGLSVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RRV4</accession>
    <name>A0A9F7RRV4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Adhesion G-protein coupled receptor G4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC128635170</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053542576.1">
        <property type="nucleotide sequence ID" value="XM_053686601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RRV4"/>
    <dbReference type="GeneID" id="128635170"/>
    <dbReference type="KEGG" id="ipu:128635170"/>
    <dbReference type="OrthoDB" id="10037534at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001759">
        <property type="entry name" value="PTX_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00354">
        <property type="entry name" value="Pentaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51828">
        <property type="entry name" value="PTX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="295"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Pentraxin (PTX)" evidence="3">
        <location>
            <begin position="45"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053542576.1"/>
        </source>
    </evidence>
    <sequence length="321" mass="35784" checksum="24E1C66ACAFD5645" modified="2023-06-28" version="1">MCVCVCVCVCVCVFSPAEVRMLNVGLCFVLFLHVTCTKDAVSEILWGKKVVLERTSCQFWLLKPNETLPSLSEFTVCVNMKRSINSSVWRAFSYLHPNRSRVELGFGGRGLQLEVQLFGRIWVTEDTLAVGHWHSVCITWSSSTSRPGLVINGRASALRKDAMEPMGRDSGTCRLAGGGSLTLGVAHYYVGDTMEVESGTNLQGSVTMFRMWGRVRSVHEIATHNCTDGDVIEWHGRVWLVQHHCTPVIDHSVECVWSWYEVKVSLCIFSYNKPILAPYEAQDITHQWVSAHFTLITLVCVCVCVCVCVCVCVCVCVCVCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TG60</accession>
    <name>A0A9F7TG60_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane protein 94 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC108258401</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053532007.1">
        <property type="nucleotide sequence ID" value="XM_053676032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TG60"/>
    <dbReference type="GeneID" id="108258401"/>
    <dbReference type="KEGG" id="ipu:108258401"/>
    <dbReference type="OrthoDB" id="5568754at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039720">
        <property type="entry name" value="TMEM94"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13219">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 94"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13219:SF6">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 94"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="824"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="856"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="899"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="964"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1002"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="1043"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="309"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="314"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053532007.1"/>
        </source>
    </evidence>
    <sequence length="1089" mass="122186" checksum="BF47E1F555B27851" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TJ34</accession>
    <name>A0A9F7TJ34_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="1">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC108261583</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="1"/>
    <dbReference type="RefSeq" id="XP_053534859.1">
        <property type="nucleotide sequence ID" value="XM_053678884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TJ34"/>
    <dbReference type="GeneID" id="108261583"/>
    <dbReference type="KEGG" id="ipu:108261583"/>
    <dbReference type="OrthoDB" id="26387at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF28">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="263"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053534859.1"/>
        </source>
    </evidence>
    <sequence length="361" mass="40672" checksum="BB8E67C4BFC53EAD" modified="2023-06-28" version="1">MVVSIKTMRECTAAAPPCEGTGHCHKETVWDKDKAKRKRKAELCWKKTMAQTLQSQSHFINKYIDLLTQDSEDLDASASTSAEHSPNSCDGALVCVGPRRWRARGGERRQMEMCILCHEAQEILPDGTAMVLAAFVQRSTVMSENRKRPPHNSERYDPLFMHPNLSFGTHTGSCGHIMHSLCWQRYFETLQSQDAQWLHEQTSYDVENGEYLCPLCKCRSNTVIPLLPLTETTCSYSNSEQPGLAHWLNTTCQQIRALHSAHKRAKPTNAGETETVEDSPPPESFRLDHTPVNPYSSSVREMLMTFGTATYKVGLKVHPNEQDPRVPIMCWGSCSCTIQSIESLLVDEEKPLFGSLPCRQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TJ62</accession>
    <name>A0A9F7TJ62_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NLR family CARD domain-containing protein 3-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC128630381</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053534856.1">
        <property type="nucleotide sequence ID" value="XM_053678881.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TJ62"/>
    <dbReference type="GeneID" id="128630381"/>
    <dbReference type="KEGG" id="ipu:128630381"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000210">
        <property type="entry name" value="Si:dkey-16p6.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="4">
        <location>
            <begin position="230"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_053534856.1"/>
        </source>
    </evidence>
    <sequence length="494" mass="56551" checksum="65265B6824A8D8C7" modified="2023-06-28" version="1">MTSNISVSGEQDLKRDERMMEGKRSDSPEPSCVSMKSDESMEHPLTFRDGASSPDVRPQQKKSNLSRNQLDSIFKELEHKVITLIKNELERFRKLLSPDYPACTEREVEDEEDLHSVRDGALKITLHVLKNMNHTDLAHTLHNKSVASVYQTKLKSSLREKFKRINEGISQHGSSALLNEIYTDLYITEGWSGDVNNEHEVRQIETASKRPAAQETPIKCNDLFKDKSIRSVLTKGVAGIGKTVSVQKFILDWTEGKANQDVTFMFPLPFRELNLMKQEHLSLMDLLHHFFPEMRKLQLIDFYKVVLIFDGLDECRLPLNFQKNERLCDVTESASVDVLLTNLIKGNLLPSALLWITSRPGAANQIPPECVDQVTEVRGFSDPQKEEYFRKRISDQSLANKVISHMKSSRSLYIMCHIPVFCWISATVLERMLGEAESGEIPKTLTQMFTHFLIFQIKHKEQKYHQKCDPDPDPDPPECSGVSGCFICIFLLYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TLU1</accession>
    <name>A0A9F7TLU1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Harmonin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ush1c</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Anchoring/scaffolding protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal development and maintenance of cochlear hair cell bundles. As part of the intermicrovillar adhesion complex/IMAC plays a role in brush border differentiation, controlling microvilli organization and length. Probably plays a central regulatory role in the assembly of the complex, recruiting CDHR2, CDHR5 and MYO7B to the microvilli tips.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Part of the IMAC/intermicrovillar adhesion complex/intermicrovillar tip-link complex composed of ANKS4B, MYO7B, USH1C, CDHR2 and CDHR5. Part of a complex composed of USH1C, USH1G and MYO7A. Interacts with F-actin. Interacts with USH2A. Interacts with SLC4A7. Interacts (via PDZ1 domain) with the C-terminus of USHBP1. Interacts (via N-terminus and PDZ 2 domain) with CDH23. Interacts with USH1G. Interacts with MYO7B. Interacts with CDHR2 and CDHR5; may mediate their interaction with MYO7B at the microvilli tip. Interacts (via PDZ 1 domain) with ANKS4B. Interacts (via PDZ 1 domain) with DOCK4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Microvillus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053541934.1">
        <property type="nucleotide sequence ID" value="XM_053685959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TLU1"/>
    <dbReference type="GeneID" id="108274436"/>
    <dbReference type="CTD" id="10083"/>
    <dbReference type="OrthoDB" id="7734647at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005903">
        <property type="term" value="C:brush border"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001917">
        <property type="term" value="C:photoreceptor inner segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002142">
        <property type="term" value="C:stereocilia ankle link complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032426">
        <property type="term" value="C:stereocilium tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002093">
        <property type="term" value="P:auditory receptor cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904970">
        <property type="term" value="P:brush border assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060122">
        <property type="term" value="P:inner ear receptor cell stereocilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050885">
        <property type="term" value="P:neuromuscular process controlling balance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904106">
        <property type="term" value="P:protein localization to microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046549">
        <property type="term" value="P:retinal cone cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07353">
        <property type="entry name" value="harmonin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06737">
        <property type="entry name" value="PDZ1_harmonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06738">
        <property type="entry name" value="PDZ2_harmonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06739">
        <property type="entry name" value="PDZ3_harmonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.20:FF:000001">
        <property type="entry name" value="harmonin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000062">
        <property type="entry name" value="harmonin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000071">
        <property type="entry name" value="harmonin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000104">
        <property type="entry name" value="harmonin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030237">
        <property type="entry name" value="Harmonin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051844">
        <property type="entry name" value="USH2_Complex_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23116:SF36">
        <property type="entry name" value="HARMONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23116">
        <property type="entry name" value="PDZ DOMAIN CONTAINING WHIRLIN AND HARMONIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21219">
        <property type="entry name" value="USH1C_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7" id="KW-1009">Hearing</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ" evidence="16">
        <location>
            <begin position="87"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="16">
        <location>
            <begin position="214"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="16">
        <location>
            <begin position="451"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="368"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="391"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00064828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_053541934.1"/>
        </source>
    </evidence>
    <sequence length="585" mass="65073" checksum="D552A5666D2D73AB" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TM51</accession>
    <name>A0A9F7TM51_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Equilibrative nucleobase transporter 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC128633444</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053537979.1">
        <property type="nucleotide sequence ID" value="XM_053682004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TM51"/>
    <dbReference type="GeneID" id="128633444"/>
    <dbReference type="KEGG" id="ipu:128633444"/>
    <dbReference type="OrthoDB" id="330047at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027197">
        <property type="entry name" value="SLC43A3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20765:SF1">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOBASE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20765">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 43 MEMBER 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="122"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="276"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="319"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="363"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="394"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="428"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="455"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="226"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="233"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053537979.1"/>
        </source>
    </evidence>
    <sequence length="495" mass="54392" checksum="4532F141F944E2B9" modified="2023-06-28" version="1">MAGAGLRVRYWLTLISGLFECLCFAGILFGWASLVFVLKSDGYFSYLCVNSTVNGTVTKDCSEQDEQLSIIFTIASFMNNFFALPNGFVFDRFGTTVTRLLGISIYTTGTLLIAFSNAALSVLLYPAVSLIAVGGILFLLTNMQVGNLFGAHRSTIITLYNGAFDSSSAVFLVVKLLYQDGVSLRSSLIFLSCCSVVHLIRTFLLMPRGHIPYALPENYTYGPGRGDARTSNELENANAKNSTRNYEATETTPFKENGPTKTATPDMSERSFRGCVFSWFFVWHLLWLSIMQLRHYLFIGTLNPMLNRLTAGDPSLVSRYTNAFAFTQLCGILCAPWNGLIMDRNKGKPREKGQSESEADLKAAVLSLCVTAMQCVLFSLCASIPLLPLQYLTFILQVINRSFLYGGNAAFISVAFPPAHFGKLYGTVMALSALLSVLQYPCFSLITGPLGGDPFAVNVALTILSCLAFIHPLSVYLHCRRRAARRTKADKTTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TPG3</accession>
    <name>A0A9F7TPG3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Condensin-2 complex subunit H2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Non-SMC condensin II complex subunit H2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">LOC108262173</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11 12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CND2 H2 (condensin-2 subunit 2) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053540334.1">
        <property type="nucleotide sequence ID" value="XM_053684359.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053540335.1">
        <property type="nucleotide sequence ID" value="XM_053684360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TPG3"/>
    <dbReference type="GeneID" id="108262173"/>
    <dbReference type="KEGG" id="ipu:108262173"/>
    <dbReference type="OrthoDB" id="10038475at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010032">
        <property type="term" value="P:meiotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051306">
        <property type="term" value="P:mitotic sister chromatid separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031737">
        <property type="entry name" value="CNDH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009378">
        <property type="entry name" value="H2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031739">
        <property type="entry name" value="Ncaph2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14324">
        <property type="entry name" value="CONDENSIN-2 COMPLEX SUBUNIT H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14324:SF3">
        <property type="entry name" value="CONDENSIN-2 COMPLEX SUBUNIT H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16858">
        <property type="entry name" value="CNDH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06278">
        <property type="entry name" value="CNDH2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Condensin II complex subunit H2 N-terminal" evidence="8">
        <location>
            <begin position="7"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Condensin-2 complex subunit H2 C-terminal" evidence="9">
        <location>
            <begin position="443"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="392"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="401"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053540334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053540335.1"/>
        </source>
    </evidence>
    <sequence length="583" mass="66676" checksum="AE60663990EED562" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TQL4</accession>
    <name>A0A9F7TQL4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Histone-lysine N-methyltransferase EHMT2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ehmt2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053541591.1">
        <property type="nucleotide sequence ID" value="XM_053685616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TQL4"/>
    <dbReference type="GeneID" id="108274790"/>
    <dbReference type="KEGG" id="ipu:108274790"/>
    <dbReference type="CTD" id="10919"/>
    <dbReference type="OrthoDB" id="5792673at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046974">
        <property type="term" value="F:histone H3K9 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002039">
        <property type="term" value="F:p53 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20905">
        <property type="entry name" value="EHMT_ZBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10533">
        <property type="entry name" value="SET_EHMT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000005">
        <property type="entry name" value="Euchromatic histone-lysine N-methyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043550">
        <property type="entry name" value="EHMT1/EHMT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047762">
        <property type="entry name" value="EHMT_CRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007728">
        <property type="entry name" value="Pre-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038034">
        <property type="entry name" value="SET_EHMT2"/>
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    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
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            <begin position="673"/>
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            <begin position="961"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
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        <source>
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    <evidence type="ECO:0000256" key="7">
        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053541591.1"/>
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    <sequence length="1199" mass="131008" checksum="B07B5CC0CA5AC775" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TSJ8</accession>
    <name>A0A9F7TSJ8_ICTPU</name>
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        <recommendedName>
            <fullName evidence="14">Netrin receptor UNC5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">unc5da</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="19">SDA103</strain>
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    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
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        <text evidence="14">Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding.</text>
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        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Single-pass type I membrane protein</topology>
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        <text evidence="2 14">Belongs to the unc-5 family.</text>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="TSP1REPEAT"/>
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        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 14" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 14 20" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="157"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="ZU5" evidence="18">
        <location>
            <begin position="518"/>
            <end position="656"/>
        </location>
    </feature>
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            <begin position="844"/>
            <end position="899"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="26"/>
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    </feature>
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        <location>
            <begin position="965"/>
            <end position="989"/>
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    </feature>
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            <begin position="968"/>
            <end position="978"/>
        </location>
    </feature>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367033"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_053543934.1"/>
        </source>
    </evidence>
    <sequence length="989" mass="108907" checksum="2B77A353E04A72AA" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>E3TG80</accession>
    <name>E3TG80_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14 16">Sumo-conjugating enzyme ubc9</fullName>
            <ecNumber evidence="14 16">6.3.2.19</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">UBC9</name>
        <name evidence="16" type="synonym">ubc9</name>
        <name evidence="16" type="synonym">ube2i</name>
        <name evidence="16" type="synonym">ube2ib</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 16" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Accepts the ubiquitin-like proteins sumo1, sumo2 and sumo3 from the uble1a-uble1b E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as ranbp2 or cbx4. Essential for nuclear architecture and chromosome segregation. Mediates nuclear localization of vsx1. Required for progression through mitosis during organogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Forms a tight complex with rangap1 and ranbp2. Interacts with vsx1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.19" evidence="14 16"/>
    <dbReference type="EMBL" id="GU589366">
        <property type="protein sequence ID" value="ADO29316.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187932.1">
        <property type="nucleotide sequence ID" value="NM_001201003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TG80"/>
    <dbReference type="SMR" id="E3TG80"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000457"/>
    <dbReference type="GeneID" id="100528733"/>
    <dbReference type="KEGG" id="ipu:100528733"/>
    <dbReference type="CTD" id="30622"/>
    <dbReference type="GeneTree" id="ENSGT00550000075088"/>
    <dbReference type="OMA" id="TWECGIP"/>
    <dbReference type="OrthoDB" id="6600758at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060216">
        <property type="term" value="P:definitive hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036306">
        <property type="term" value="P:embryonic heart tube elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001947">
        <property type="term" value="P:heart looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23798">
        <property type="entry name" value="UBCc_UBE2I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000013">
        <property type="entry name" value="SUMO-conjugating enzyme UBC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="14" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="13">
        <location>
            <begin position="4"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="11">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ADO29316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="NP_001187932.1"/>
        </source>
    </evidence>
    <sequence length="158" mass="18023" checksum="E2C83E89C8D0683D" modified="2011-01-11" version="1">MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-09-05" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I4IY93</accession>
    <name>I4IY93_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Tachykinin 3</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="12">Tachykinin-3a isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tac3</name>
        <name evidence="12" type="synonym">tac3a</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="10269" last="10274">
            <title>Neurokinin Bs and neurokinin B receptors in zebrafish-potential role in controlling fish reproduction.</title>
            <authorList>
                <person name="Biran J."/>
                <person name="Palevitch O."/>
                <person name="Ben-Dor S."/>
                <person name="Levavi-Sivan B."/>
            </authorList>
            <dbReference type="PubMed" id="22689988"/>
            <dbReference type="DOI" id="10.1073/pnas.1119165109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles. Is a critical central regulator of gonadal function.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tachykinin family.</text>
    </comment>
    <dbReference type="EMBL" id="BK008101">
        <property type="protein sequence ID" value="DAA35153.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017343311.1">
        <property type="nucleotide sequence ID" value="XM_017487822.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I4IY93"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012902"/>
    <dbReference type="GeneID" id="108276280"/>
    <dbReference type="KEGG" id="ipu:108276280"/>
    <dbReference type="CTD" id="100320280"/>
    <dbReference type="GeneTree" id="ENSGT00390000000335"/>
    <dbReference type="OMA" id="EPENGPW"/>
    <dbReference type="OrthoDB" id="9397481at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045777">
        <property type="term" value="P:positive regulation of blood pressure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007217">
        <property type="term" value="P:tachykinin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003635">
        <property type="entry name" value="Neurokinin-B/TAC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013055">
        <property type="entry name" value="Tachy_Neuro_lke_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15536">
        <property type="entry name" value="TACHYKININ-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15536:SF1">
        <property type="entry name" value="TACHYKININ-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00267">
        <property type="entry name" value="TACHYKININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0027">Amidation</keyword>
    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="7" id="KW-0527">Neuropeptide</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013527457" evidence="9">
        <location>
            <begin position="22"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="DAA35153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017343311.1"/>
        </source>
    </evidence>
    <sequence length="127" mass="14887" checksum="C71E3A45912EBB15" modified="2012-09-05" version="1">MYRGLLLVFLILLVETRLSESSCAEQETQRSASDESSSFRLATRNLLKRYHDIDYDSFVGLMGRRSADAAAAAEDQSQRKREMHDIFVGLMGRRNSEDDTERPWRKDYPETRGLFFNKCRLRFRRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-12-01" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>Q90YW7</accession>
    <name>Q90YW7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5 7">Ribosomal protein L4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC100304519</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2001-07" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Patterson A.P."/>
                <person name="Karsi A."/>
                <person name="Liu Z.J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="5 7" key="2">
        <citation type="journal article" date="2003" name="Gene" volume="305" first="151" last="160">
            <title>Translational machinery of channel catfish: II. Complementary DNA and expression of the complete set of 47 60S ribosomal proteins.</title>
            <authorList>
                <person name="Patterson A."/>
                <person name="Karsi A."/>
                <person name="Feng J."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="12609735"/>
            <dbReference type="DOI" id="10.1016/S0378-1119(02)01183-6"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AF401555">
        <property type="protein sequence ID" value="AAK95127.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187034.1">
        <property type="nucleotide sequence ID" value="NM_001200105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q90YW7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032035"/>
    <dbReference type="KEGG" id="ipu:100304519"/>
    <dbReference type="OrthoDB" id="10259785at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1370.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025755">
        <property type="entry name" value="Ribos_uL4_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_uL4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045240">
        <property type="entry name" value="Ribosomal_uL4_euk/arch"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19431">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14374">
        <property type="entry name" value="Ribos_L4_asso_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="Ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL4 C-terminal" evidence="4">
        <location>
            <begin position="276"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAK95127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="NP_001187034.1"/>
        </source>
    </evidence>
    <sequence length="385" mass="43728" checksum="7180C48E794153AF" modified="2001-12-01" version="1">MACARPLISVYSDKGETSGKNVVLPAVFTAPIRPDVVNFVHTNMRKNKRHAYAVSEQAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKTWRRWHRRINVTQKRYAICSALAGSALPALVMAKGHRIEEIPEVPLVVDDKVEGYKKTKEAVLLLKKLKAWNDIKKVYASQRMRAGKGKMRNGRRIQRKGPCIIYNEDNGVTKAFRNIPGVTMQRVTKLNLLKLAPGGHIGRFCIWTEAAFRKLDDLYGTWRKPSSLKVDYNLPMHKMTNTDLGRILQSEEIQKAVRAPNKTIQRRVLKKNPLKNLRIMMKLNPYAKTARRHAILKHKPEIKAKMLKPKVKRVGRLRRSRLQQLLLQQSKVVCILKLCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5U758</accession>
    <name>W5U758_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BICD family-like cargo adapter 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Bicaudal D-related protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Coiled-coil domain-containing protein 64B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ccdc64b</name>
        <name evidence="9" type="synonym">bicdl2</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT406596">
        <property type="protein sequence ID" value="AHH37650.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017315313.1">
        <property type="nucleotide sequence ID" value="XM_017459824.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U758"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033500"/>
    <dbReference type="GeneID" id="108259965"/>
    <dbReference type="KEGG" id="ipu:108259965"/>
    <dbReference type="CTD" id="146439"/>
    <dbReference type="GeneTree" id="ENSGT00940000160797"/>
    <dbReference type="OMA" id="TIHQKVA"/>
    <dbReference type="OrthoDB" id="9451547at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055107">
        <property type="term" value="P:Golgi to secretory granule transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047496">
        <property type="term" value="P:vesicle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051149">
        <property type="entry name" value="Spindly/BICDR_Dynein_Adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32123">
        <property type="entry name" value="BICD FAMILY-LIKE CARGO ADAPTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32123:SF11">
        <property type="entry name" value="BICD FAMILY-LIKE CARGO ADAPTER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="405"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="194"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="303"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="127"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="418"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00041790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00043196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AHH37650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017315313.1"/>
        </source>
    </evidence>
    <sequence length="447" mass="52014" checksum="D900EDBE6327ABC0" modified="2014-04-16" version="1">MISTRKESLTSPTLEDSFFPFSSSTPVSRHRIMGLGVRANNLLASHTDQDSSSTMLEKDLILAAEVGQALLERNEELQAQLEQMRRDIEALQQEKHTLQRHLEVQELEAGQREAELQADIASLRQQYDQKHNQGRDRRREESEQLTQLSNHNQKLVEQLAEAVSVEHSLRLELRSLKEEMEDTSFSRSINSARLDSLQAENQVLKERFSNMDERLKSSDEDNERLRMEREGLRARLLELQTDLKEKECELEQHQSEAFQLRTMNRTLQQKVQAFGEEASLEETTCFPLSLQNELQQSQAKESIFAHSAVLQEKDEEIERLKKELESNQYELAALRDEVKLFRSDPSKPTYSALEEELAVARQERDTLNQQLLNTIRHKVALSQEVESWQEDMRLVISQQVQQQVHEREKEKQKMASPLQRNIRTSKSFRLPREEGKKNFFSSLFGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5U8S6</accession>
    <name>W5U8S6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9 11 12">Bromodomain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">BRD3</name>
        <name evidence="11 12" type="synonym">brd3a</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11 12">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the BET family.</text>
    </comment>
    <dbReference type="EMBL" id="JT407406">
        <property type="protein sequence ID" value="AHH37974.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017347843.1">
        <property type="nucleotide sequence ID" value="XM_017492354.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053543720.1">
        <property type="nucleotide sequence ID" value="XM_053687745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U8S6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018392"/>
    <dbReference type="GeneID" id="108278779"/>
    <dbReference type="KEGG" id="ipu:108278779"/>
    <dbReference type="CTD" id="327372"/>
    <dbReference type="GeneTree" id="ENSGT00940000162790"/>
    <dbReference type="OMA" id="QIVNPPP"/>
    <dbReference type="OrthoDB" id="21449at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05497">
        <property type="entry name" value="Bromo_Brdt_I_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05498">
        <property type="entry name" value="Bromo_Brdt_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000003">
        <property type="entry name" value="Bromodomain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.220:FF:000001">
        <property type="entry name" value="bromodomain-containing protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000002">
        <property type="entry name" value="Bromodomain-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043508">
        <property type="entry name" value="Bromo_Brdt_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043509">
        <property type="entry name" value="Bromo_Brdt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050935">
        <property type="entry name" value="Bromo_chromatin_reader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027353">
        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038336">
        <property type="entry name" value="NET_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880:SF246">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880">
        <property type="entry name" value="FALZ-RELATED BROMODOMAIN-CONTAINING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17035">
        <property type="entry name" value="BET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51525">
        <property type="entry name" value="NET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3 5" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Bromo" evidence="7">
        <location>
            <begin position="47"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="7">
        <location>
            <begin position="308"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="NET" evidence="8">
        <location>
            <begin position="544"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="144"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="416"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="465"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="618"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="160"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="181"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="230"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="416"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="474"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="491"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="506"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="642"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AHH37974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017347843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053543720.1"/>
        </source>
    </evidence>
    <sequence length="666" mass="72868" checksum="C087A57F600AD9C4" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>W5UAA0</accession>
    <name>W5UAA0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD(+) kinase</fullName>
            <ecNumber evidence="2">2.7.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">Nadk</name>
        <name evidence="10" type="synonym">nadkb</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="2"/>
    <dbReference type="EMBL" id="JT409571">
        <property type="protein sequence ID" value="AHH38735.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053537134.1">
        <property type="nucleotide sequence ID" value="XM_053681159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UAA0"/>
    <dbReference type="KEGG" id="ipu:108267451"/>
    <dbReference type="GeneTree" id="ENSGT00940000165431"/>
    <dbReference type="OrthoDB" id="24581at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.30:FF:000003">
        <property type="entry name" value="NAD kinase b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF29">
        <property type="entry name" value="NAD(+) KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 8 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AHH38735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053537134.1"/>
        </source>
    </evidence>
    <sequence length="456" mass="50544" checksum="9E8874BEC6897C94" modified="2014-04-16" version="1">MECSDENQPDEMPEEQICGTEGHRKSPKRRREIKRSRRRVNGVEQACSGSELRGDSELHDASETSESSTKRLDKCRRAHFLHGPYPSTHFGPKACILPNPTSIMHIQDPVSQRLVWNKPPLNVLVIRKIRDENLLEPFKELCSFLIQEKGLSVYVEKKVVEDGALMADESFSSIRRQLCTFREGFDDISDCIDLIVCLGGDGTLLYASSLFQGSVPPVMAFHLGSLGFLTPFNFETYKTEVDKVFAGNAAVVLRSRLKVKVLKGSLQRDQENGSVTHTHTHTNPVSLQLQVLNEVVVDRGPSSYLSNVDLYLDGRLITSVQGDGVIVSTPTGSTAYAAAAGASMIHPNVPAIMVTPICPHSLSFRPIVVPAGVELMIALSPDARNTAWVSFDGRKRQEIQHGDSIKITTSCYPVPSLCRHGLVYDWFESLAECLHWNIRKKQTHLSDASDSTDTDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>W5UBI2</accession>
    <name>W5UBI2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Dual serine/threonine and tyrosine protein kinase</fullName>
            <ecNumber evidence="5">2.7.12.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Dusty protein kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Receptor-interacting serine/threonine-protein kinase 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">dstyk</name>
        <name evidence="26" type="synonym">LOC108281089</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="24">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="journal article" date="2016" name="Biol. Reprod." volume="95" first="19" last="19">
            <title>Transcriptome Display During Testicular Differentiation of Channel Catfish (Ictalurus punctatus) as Revealed by RNA-Seq Analysis.</title>
            <authorList>
                <person name="Zeng Q."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Zhang Y."/>
                <person name="Yuan Z."/>
                <person name="Jiang C."/>
                <person name="Chen A."/>
                <person name="Fu Q."/>
                <person name="Su B."/>
                <person name="Dunham R."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="27307075"/>
            <dbReference type="DOI" id="10.1095/biolreprod.116.138818"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="5"/>
    <dbReference type="EMBL" id="JT411309">
        <property type="protein sequence ID" value="AHH39376.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053535334.1">
        <property type="nucleotide sequence ID" value="XM_053679359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UBI2"/>
    <dbReference type="KEGG" id="ipu:108281089"/>
    <dbReference type="OrthoDB" id="122279at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044344">
        <property type="term" value="P:cellular response to fibroblast growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070374">
        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045743">
        <property type="term" value="P:positive regulation of fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13975">
        <property type="entry name" value="PKc_Dusty"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000244">
        <property type="entry name" value="Dual serine/threonine and tyrosine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051302">
        <property type="entry name" value="Dual_SerThr-Tyr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46392">
        <property type="entry name" value="DUAL SERINE/THREONINE AND TYROSINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46392:SF1">
        <property type="entry name" value="DUAL SERINE_THREONINE AND TYROSINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="12 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 24 26" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="631"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="660"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00040421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="AHH39376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_053535334.1"/>
        </source>
    </evidence>
    <sequence length="907" mass="101617" checksum="BC4F9F476AD5A3C8" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5UC02</accession>
    <name>W5UC02_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ephrin-A4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">EPH-related receptor tyrosine kinase ligand 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">Efna4</name>
        <name evidence="18" type="synonym">si:dkey-246i14.3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. May play a role in the interaction between activated B-lymphocytes and dendritic cells in tonsils.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12 13">Belongs to the ephrin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="JT411670">
        <property type="protein sequence ID" value="AHH39546.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017330541.1">
        <property type="nucleotide sequence ID" value="XM_017475052.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UC02"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005286"/>
    <dbReference type="GeneID" id="108269281"/>
    <dbReference type="KEGG" id="ipu:108269281"/>
    <dbReference type="GeneTree" id="ENSGT00940000160040"/>
    <dbReference type="OrthoDB" id="6250301at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046875">
        <property type="term" value="F:ephrin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048013">
        <property type="term" value="P:ephrin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10425">
        <property type="entry name" value="Ephrin-A_Ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000057">
        <property type="entry name" value="Ephrin A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031328">
        <property type="entry name" value="Ephrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034252">
        <property type="entry name" value="Ephrin-A_Ecto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019765">
        <property type="entry name" value="Ephrin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001799">
        <property type="entry name" value="Ephrin_RBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11304">
        <property type="entry name" value="EPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11304:SF42">
        <property type="entry name" value="EPHRIN-A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00812">
        <property type="entry name" value="Ephrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01347">
        <property type="entry name" value="EPHRIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01299">
        <property type="entry name" value="EPHRIN_RBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51551">
        <property type="entry name" value="EPHRIN_RBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540141" description="Ephrin-A4" evidence="14">
        <location>
            <begin position="33"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Ephrin RBD" evidence="15">
        <location>
            <begin position="32"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00078735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AHH39546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017330541.1"/>
        </source>
    </evidence>
    <sequence length="206" mass="22955" checksum="B9A120BAEBA0D53E" modified="2014-04-16" version="1">MQIMSFLQFSSGPSHTFTLLILLMHVHSSAVAKRHMVYWNSTNTRLTSDDYSVQVNLNDYLDILCPHYPSGSPEKGPPETLALYLVAENQFQGCHETEGAIKRWECNSPYAPFGPVRFSEKIQRFTPFSLGFEFLPGHHYYYSSLSVDDGPPLPCMKLKVTVCCESTATDGKKVEGTPAPHSFAQPGRTSPLPVLALTSFSLFCLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5UFV0</accession>
    <name>W5UFV0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Sodium channel regulatory subunit beta-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Sodium channel regulatory subunit beta-3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">SCN1B</name>
        <name evidence="30" type="synonym">scn1bb</name>
    </gene>
    <organism evidence="28">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="28">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="29" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="29">SDA103</strain>
        </source>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="30">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="23">Regulatory subunit of multiple voltage-gated sodium (Nav) channels directly mediating the depolarization of excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na+ ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues. The accessory beta subunits participate in localization and functional modulation of the Nav channels. Modulates the activity of SCN1A/Nav1.1, SCN2A/Nav1.2, SCN3A/Nav1.3, SCN4A/Nav1.4, SCN5A/Nav1.5, SCN8A/Nav1.6, SCN9A/Nav1.7 and SCN10A/Nav1.8.</text>
    </comment>
    <comment type="subunit">
        <text evidence="25">A voltage-gated sodium (Nav) channel consists of an ion-conducting pore-forming alpha subunit functional on its own that is regulated by one or more beta subunits. Forms homodimers and homotrimers. SCN3B is non-covalently associated with alpha subunits and induces the formation of alpha subunit oligomers, including trimers. Interacts with SCN5A/Nav1.5; regulatory subunit of SCN5A/Nav1.5. Interacts with SCN7A/Nav2.1; probable regulatory subunit of SCN7A/Nav2.1. Interacts with SCN10A; regulatory subunit of SCN10A/Nav1.8. Interacts with NFASC; probably involved in targeting the sodium channels to the nodes of Ranvier.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Perikaryon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the sodium channel auxiliary subunit SCN1B (TC 8.A.17) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sodium channel auxiliary subunit SCN3B (TC 8.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="JT413980">
        <property type="protein sequence ID" value="AHH40449.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017340176.1">
        <property type="nucleotide sequence ID" value="XM_017484687.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UFV0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000010483"/>
    <dbReference type="GeneID" id="108274487"/>
    <dbReference type="KEGG" id="ipu:108274487"/>
    <dbReference type="CTD" id="100005325"/>
    <dbReference type="GeneTree" id="ENSGT00390000018560"/>
    <dbReference type="OMA" id="VWGGCVE"/>
    <dbReference type="OrthoDB" id="8868224at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005272">
        <property type="term" value="F:sodium channel activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:sodium channel inhibitor activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:transmembrane transporter binding"/>
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    </dbReference>
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        <property type="term" value="P:cardiac muscle cell action potential involved in contraction"/>
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    </dbReference>
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        <property type="term" value="P:regulation of heart rate by cardiac conduction"/>
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        <property type="entry name" value="Sodium channel beta 1 subunit"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig_V-set"/>
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    <dbReference type="InterPro" id="IPR027098">
        <property type="entry name" value="Na_channel_b1/b3"/>
    </dbReference>
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        <property type="entry name" value="SODIUM CHANNEL SUBUNIT BETA-1"/>
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        <property type="entry name" value="SODIUM CHANNEL SUBUNIT BETA-1 AND 3"/>
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    </dbReference>
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        <property type="entry name" value="V-set"/>
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    </dbReference>
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        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="20" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="19 28 30" id="KW-0407">Ion channel</keyword>
    <keyword evidence="13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 26" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0915">Sodium</keyword>
    <keyword evidence="6" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="17" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540252" description="Sodium channel regulatory subunit beta-1" evidence="26">
        <location>
            <begin position="37"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="27">
        <location>
            <begin position="47"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00010404"/>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00045714"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="EMBL" id="AHH40449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="RefSeq" id="XP_017340176.1"/>
        </source>
    </evidence>
    <sequence length="232" mass="26044" checksum="3539592CE76FC101" modified="2014-04-16" version="1">MVQTALHFLSGMSARYFLFLCVLCALWVSLCNGACAEVDSDTEAVAGQGFKLGCISCKMRGEVKARVTVDWYFRAKGEKEFIHIYTYGEEATLVIEERFTDRVDWNGSRSKLNDLQDASIYIHNVTFNDSGVYRCSFRRTLLYEAYEYETTATKLVHLTVVAKATRGTASIVSEVMMYVSIIGLQLWLLVEMIYCYRKIAAAGDEALRVSAEEYLAIASESKDNCTGVQVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5UGF1</accession>
    <name>W5UGF1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4 6">Kinesin-associated protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">KIFAP3</name>
        <name evidence="6" type="synonym">kifap3a</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
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        <property type="protein sequence ID" value="AHH40975.1"/>
        <property type="molecule type" value="mRNA"/>
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        <property type="nucleotide sequence ID" value="XM_017456310.3"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
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        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016939">
        <property type="term" value="C:kinesin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
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        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008658">
        <property type="entry name" value="KAP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15605:SF2">
        <property type="entry name" value="KINESIN-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15605">
        <property type="entry name" value="KINESIN-ASSOCIATED PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05804">
        <property type="entry name" value="KAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM01297">
        <property type="entry name" value="KAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="189"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="1">
        <location>
            <begin position="587"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="225"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AHH40975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017311799.1"/>
        </source>
    </evidence>
    <sequence length="789" mass="90134" checksum="D4C4E6AD50DBBD16" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5UHT7</accession>
    <name>W5UHT7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Teashirt 3</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="20">Teashirt homolog 3b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">TSHZ3</name>
        <name evidence="20" type="synonym">tshz3b</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the teashirt C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="JT415441">
        <property type="protein sequence ID" value="AHH41694.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017314268.1">
        <property type="nucleotide sequence ID" value="XM_017458779.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UHT7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030900"/>
    <dbReference type="GeneID" id="108259359"/>
    <dbReference type="KEGG" id="ipu:108259359"/>
    <dbReference type="CTD" id="565811"/>
    <dbReference type="OMA" id="VIADDKM"/>
    <dbReference type="OrthoDB" id="5815793at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027008">
        <property type="entry name" value="Teashirt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058631">
        <property type="entry name" value="TSHZ1-3_homeodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12487:SF5">
        <property type="entry name" value="TEASHIRT HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12487">
        <property type="entry name" value="TEASHIRT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26094">
        <property type="entry name" value="HTH_TSHZ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 14" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="302"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="16">
        <location>
            <begin position="931"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="1112"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="14">
        <location>
            <begin position="933"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="58"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="156"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="352"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="503"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="661"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="830"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="898"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1081"/>
            <end position="1105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="265"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="373"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="503"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="687"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="711"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="839"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="898"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="912"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1081"/>
            <end position="1101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AHH41694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017314268.1"/>
        </source>
    </evidence>
    <sequence length="1152" mass="126177" checksum="4D60A16D1A6B4EA8" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5UIH8</accession>
    <name>W5UIH8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Beta-1,4-galactosyltransferase</fullName>
            <shortName evidence="18">Beta-1,4-GalTase</shortName>
            <ecNumber evidence="18">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">B4galt6</name>
        <name evidence="23" type="synonym">b4galt6</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine + UDP-alpha-D-galactose = a beta-D-Gal-(1-&gt;4)-beta-D-Glc-(1&lt;-&gt;1)-Cer(d18:1(4E)) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17950"/>
            <dbReference type="ChEBI" id="CHEBI:22801"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="2.4.1.274"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:31496"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 18">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Golgi apparatus membrane</location>
            <topology evidence="18">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">Golgi stack membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the glycosyltransferase 7 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="18"/>
    <dbReference type="EMBL" id="JT415900">
        <property type="protein sequence ID" value="AHH41857.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017311719.1">
        <property type="nucleotide sequence ID" value="XM_017456230.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UIH8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021957"/>
    <dbReference type="GeneID" id="108258006"/>
    <dbReference type="KEGG" id="ipu:108258006"/>
    <dbReference type="CTD" id="9331"/>
    <dbReference type="GeneTree" id="ENSGT00940000158019"/>
    <dbReference type="OMA" id="HFLYKNI"/>
    <dbReference type="OrthoDB" id="10038994at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008489">
        <property type="term" value="F:UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006688">
        <property type="term" value="P:glycosphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00899">
        <property type="entry name" value="b4GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000037">
        <property type="entry name" value="Beta-1,4-galactosyltransferase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003859">
        <property type="entry name" value="Galactosyl_T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027791">
        <property type="entry name" value="Galactosyl_T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027995">
        <property type="entry name" value="Galactosyl_T_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300:SF47">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02709">
        <property type="entry name" value="Glyco_transf_7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13733">
        <property type="entry name" value="Glyco_transf_7N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02050">
        <property type="entry name" value="B14GALTRFASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15 18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 18 21" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16 18" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 18" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="7 18 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Galactosyltransferase N-terminal" evidence="20">
        <location>
            <begin position="108"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Galactosyltransferase C-terminal" evidence="19">
        <location>
            <begin position="246"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU368121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF13733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="AHH41857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017311719.1"/>
        </source>
    </evidence>
    <sequence length="381" mass="44494" checksum="47AFE60473402C7F" modified="2014-04-16" version="1">MLNSRRLLRLSNRSFLAFFFFFSMSTTCLYFIYVAPGIANTYLFMVQAQGIMLRDNVRTIGQIIRSYTNKNSTLNGTDYPDGNNSSEYFAQPTTYLPENFTYAQNLPCPERLPSMKGPTEVNMTELPIEEVELQHRQLGIQFGGHWRPKDCKPRWKVAILIPFRNRHEHLPILFQHLAPMLQRQRLQFAFYVIEQAGTQPFNRAMLFNVGFKEAMKDLDWDCVVFHDVDHIPENDRNYYGCGQMPRHFATKLDKYMYILPYSEFFGGVSGLTVEQFRKINGFPNAFWGWGGEDDDLWNRVHFIGLNVTRPEGDIGKYKSIPHHHRGEVQFLGRYKLLRYSKERQYLDGLNNLQYSPEISLSSLYKNITVNLSPELAPIAEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5ULC1</accession>
    <name>W5ULC1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4 6">Microfibril-associated glycoprotein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MFAP4</name>
        <name evidence="6" type="synonym">LOC108258806</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT419075">
        <property type="protein sequence ID" value="AHH42944.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017313232.1">
        <property type="nucleotide sequence ID" value="XM_017457743.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ULC1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000016755"/>
    <dbReference type="GeneID" id="108258806"/>
    <dbReference type="KEGG" id="ipu:108258806"/>
    <dbReference type="OMA" id="NCQRTNP"/>
    <dbReference type="OrthoDB" id="7735550at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048251">
        <property type="term" value="P:elastic fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.215.10:FF:000001">
        <property type="entry name" value="Tenascin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF225">
        <property type="entry name" value="MICROFIBRIL-ASSOCIATED GLYCOPROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540435" evidence="2">
        <location>
            <begin position="24"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="3">
        <location>
            <begin position="26"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AHH42944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017313232.1"/>
        </source>
    </evidence>
    <sequence length="251" mass="28627" checksum="4771C52854F754FC" modified="2014-04-16" version="1">MIGAVVSVISVFWTLLLPLLVGSTPISQELFPTDCSDVYANGQTLSGVYTIYPTADTPVQVYCDMGCLESKTEDGNWTVFQRRMDGSVNFYRPWGHYKKGFGNKYGEYWLGLENLYQLTRNRKYELRVDLQDFDGVSVYAQYSYFSVESEAEGYKLHVSGFINGGAGDSLEFINGQKFSTFDKDQDLNEEGNCAKSYLGAFWYNKCHHANPNGIYLWGRDATHFAIGNVWHHWKGYDYGLKFITMKIRPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5ULH4</accession>
    <name>W5ULH4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15 17">EH domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Ehd3</name>
        <name evidence="17" type="synonym">ehd3</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT414238">
        <property type="protein sequence ID" value="AHH40547.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017313237.1">
        <property type="nucleotide sequence ID" value="XM_017457748.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ULH4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006672"/>
    <dbReference type="GeneID" id="108258807"/>
    <dbReference type="KEGG" id="ipu:108258807"/>
    <dbReference type="CTD" id="30845"/>
    <dbReference type="GeneTree" id="ENSGT00940000159274"/>
    <dbReference type="OMA" id="LMIGQYS"/>
    <dbReference type="OrthoDB" id="1716625at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09913">
        <property type="entry name" value="EHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000147">
        <property type="entry name" value="EH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000038">
        <property type="entry name" value="EH domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040990">
        <property type="entry name" value="DUF5600"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031692">
        <property type="entry name" value="EHD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF67">
        <property type="entry name" value="EH DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18150">
        <property type="entry name" value="DUF5600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16880">
        <property type="entry name" value="EHD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="14">
        <location>
            <begin position="55"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="12">
        <location>
            <begin position="444"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="476"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AHH40547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017313237.1"/>
        </source>
    </evidence>
    <sequence length="535" mass="60733" checksum="23D9A4EB1658EA68" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5USJ2</accession>
    <name>W5USJ2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Stathmin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Stmn4</name>
        <name evidence="7 8 9 10" type="synonym">stmn4</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7 8 9" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7 8 9">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the stathmin family.</text>
    </comment>
    <dbReference type="EMBL" id="JT418053">
        <property type="protein sequence ID" value="AHH42598.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017310917.1">
        <property type="nucleotide sequence ID" value="XM_017455428.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047006939.1">
        <property type="nucleotide sequence ID" value="XM_047150983.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047006940.1">
        <property type="nucleotide sequence ID" value="XM_047150984.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047006941.1">
        <property type="nucleotide sequence ID" value="XM_047150985.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5USJ2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000011629"/>
    <dbReference type="GeneID" id="108257558"/>
    <dbReference type="KEGG" id="ipu:108257558"/>
    <dbReference type="CTD" id="81551"/>
    <dbReference type="GeneTree" id="ENSGT01030000234597"/>
    <dbReference type="OMA" id="LGWCAIK"/>
    <dbReference type="OrthoDB" id="5986631at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007019">
        <property type="term" value="P:microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031110">
        <property type="term" value="P:regulation of microtubule polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030514">
        <property type="entry name" value="Stathmin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000956">
        <property type="entry name" value="Stathmin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036002">
        <property type="entry name" value="Stathmin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10104">
        <property type="entry name" value="STATHMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10104:SF6">
        <property type="entry name" value="STATHMIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00836">
        <property type="entry name" value="Stathmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002285">
        <property type="entry name" value="Stathmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00345">
        <property type="entry name" value="STATHMIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101494">
        <property type="entry name" value="Stathmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01041">
        <property type="entry name" value="STATHMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51663">
        <property type="entry name" value="STATHMIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AHH42598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017310917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_047006939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_047006940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_047006941.1"/>
        </source>
    </evidence>
    <sequence length="188" mass="22018" checksum="9C510D6F8E6CA624" modified="2014-04-16" version="1">MTLAAYRDKLKELPLVSLFCSCIKEDITEKPTYKAEDAVDLNWCEIKDVEVIELNKRASGQAFEVILKPPSFDGVPELNATMPHRKDPSLEEIQKKLEAAEERRKFQEAELLKHLAEKREHEREVIQKAFEENNNFIKNAKEKLEQKMETNKENREALLAAMLERLQEKDKHAEEVRKNKELKEEACR</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W6A302</accession>
    <name>W6A302_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP-binding cassette sub-family F member 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">Abcf3</name>
        <name evidence="13" type="synonym">abcf3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2013" name="PLoS ONE" volume="8" first="E63895" last="E63895">
            <title>Genome-wide identification, characterization and phylogenetic analysis of 50 catfish ATP-binding cassette (ABC) transporter genes.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Li Q."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23696857"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="JT409535">
        <property type="protein sequence ID" value="AHI50412.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017328243.1">
        <property type="nucleotide sequence ID" value="XM_017472754.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W6A302"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000016414"/>
    <dbReference type="GeneID" id="108268070"/>
    <dbReference type="KEGG" id="ipu:108268070"/>
    <dbReference type="CTD" id="55324"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000104">
        <property type="entry name" value="ATP-binding cassette sub-family F member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000688">
        <property type="entry name" value="ATP-binding cassette sub-family F member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058770">
        <property type="entry name" value="PWI_ABCF3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF117">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26051">
        <property type="entry name" value="PWI_ABCF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="5 11 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="179"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="493"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="129"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="129"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AHI50412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017328243.1"/>
        </source>
    </evidence>
    <sequence length="710" mass="79946" checksum="0F053B0296301FED" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2015-04-01" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0B5GUN5</accession>
    <name>A0A0B5GUN5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Claudin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CLDN15b</name>
        <name evidence="12" type="synonym">cldn15b</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="10 12" key="2">
        <citation type="journal article" date="2015" name="Comp. Biochem. Physiol. Part D Genomics Proteomics" volume="13" first="60" last="69">
            <title>Claudin multigene family in channel catfish and their expression profiles in response to bacterial infection and hypoxia as revealed by meta-analysis of RNA-Seq datasets.</title>
            <authorList>
                <person name="Sun L."/>
                <person name="Liu S."/>
                <person name="Bao L."/>
                <person name="Li Y."/>
                <person name="Feng J."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="25681604"/>
            <dbReference type="DOI" id="10.1016/j.cbd.2015.01.002"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell junction</location>
            <location evidence="8">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the claudin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="KM870796">
        <property type="protein sequence ID" value="AJF34910.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001316184.1">
        <property type="nucleotide sequence ID" value="NM_001329255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A0B5GUN5"/>
    <dbReference type="GeneID" id="108260187"/>
    <dbReference type="KEGG" id="ipu:108260187"/>
    <dbReference type="CTD" id="678556"/>
    <dbReference type="OrthoDB" id="9933182at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
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        <location>
            <begin position="1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="NP_001316184.1"/>
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    <sequence length="223" mass="24696" checksum="7500CFCAD766F8FD" modified="2015-04-01" version="1">MRESLQIVALFLGFLSGVTAFISLHHHTWKVSTDYGNVIITSNIFENLWMSCAEDSTGIYDCWYFSSLLALPGFVQACRALMISSIVLGTFGLIFTLVGMQCSKIGGENYIVKGRIAVLGGVFFILQGLSTMIAVSWYAFNITQEFFDPLYPGTKFEIGEGLYIGWCSATLALIGGCCLLCACGKNNKKEKMSYPYQSQRRGTVYTTSIMSQQPNNYGRNAYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PIH8</accession>
    <name>A0A2D0PIH8_ICTPU</name>
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        <submittedName>
            <fullName evidence="10">Transcription factor SOX-13 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC108255132</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
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    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="10">Blood</tissue>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="High mobility group box domain"/>
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    </dbReference>
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        <property type="entry name" value="SOX/SOX-like_TF"/>
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        <property type="entry name" value="FI18025P1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRANSCRIPTION FACTOR SOX-13"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="8">
        <location>
            <begin position="467"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="5">
        <location>
            <begin position="467"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="7">
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            <end position="134"/>
        </location>
    </feature>
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            <end position="350"/>
        </location>
    </feature>
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    </feature>
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        </location>
    </feature>
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            <begin position="218"/>
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        </location>
    </feature>
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    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017306328.1"/>
        </source>
    </evidence>
    <sequence length="655" mass="73679" checksum="968A9133F49018EB" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PL00</accession>
    <name>A0A2D0PL00_ICTPU</name>
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        <recommendedName>
            <fullName evidence="17">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">slc6a6b</name>
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    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>4-aminobutanoate(out) + chloride(out) + 2 Na(+)(out) = 4-aminobutanoate(in) + chloride(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:70687"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:70688"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>beta-alanine(out) + chloride(out) + 2 Na(+)(out) = beta-alanine(in) + chloride(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71247"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:71248"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>hypotaurine(out) + chloride(out) + 2 Na(+)(out) = hypotaurine(in) + chloride(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71243"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:57853"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:71244"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>taurine(out) + chloride(out) + 2 Na(+)(out) = taurine(in) + chloride(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71223"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:507393"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:71224"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A6 subfamily.</text>
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            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the paired homeobox family. Unc-4 subfamily.</text>
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        <property type="nucleotide sequence ID" value="XM_017454074.3"/>
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    <dbReference type="GeneID" id="108256852"/>
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    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000057">
        <property type="entry name" value="Short stature homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46799">
        <property type="entry name" value="HOMEOBOX PROTEIN UNC-4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46799:SF1">
        <property type="entry name" value="HOMEOBOX PROTEIN UNC-4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6 11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 11 12" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9 11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="98"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="100"/>
            <end position="159"/>
        </location>
    </feature>
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        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
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            <end position="248"/>
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    </feature>
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        <location>
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        <location>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="464"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017309563.1"/>
        </source>
    </evidence>
    <sequence length="476" mass="51463" checksum="B1CA5CB0BB7AC350" modified="2017-12-20" version="1">MMDSRILDPPHAQFGGSLGGMVSFPYHLGHHHVYELAGHQLQSAAAVPFSIDGLLNGSCAASVVNSNPLLSSGCAMNGDNQQYKLTDSGDPEKDSPGCKRRRTRTNFTGWQLEELEKAFNESHYPDVFMREALALRLDLIESRVQVWFQNRRAKWRKKENTKKGPGRPAHNSHPTTCSGEPMDAEEIARRERERLEKKKRKQEKRLLRAQGGKLLPGDLFHTPGSDSDSGVSHVTDSEHQQVHCDSVSRHQTHAGCEQTSNQRSAHADASGAELDSSDGARQASLARSPGLQKANPFSVESLLSDSRPRRKPAVDFPVASARPLIGKGHFLLYPITQPLGFIVPQTALKGTAAGTEEEHEHGPKVLVSDSDATGSPVQRSDPELSSVNPNATAIKGQVSPSGPLACSLQASPGQACNGPSSGEVCPPDREPELGCPKSPQSEKKEQSLGVFDYPQSSESPSTHTDTDKDSADVDMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PVV4</accession>
    <name>A0A2D0PVV4_ICTPU</name>
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        <submittedName>
            <fullName evidence="7">Integrator complex subunit 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ints1</name>
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    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
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    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017310147.1">
        <property type="nucleotide sequence ID" value="XM_017454658.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000027758"/>
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    <dbReference type="KEGG" id="ipu:108257161"/>
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    <dbReference type="OrthoDB" id="19938at2759"/>
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        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034474">
        <property type="term" value="P:U2 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
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        <property type="entry name" value="INT1_R3"/>
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    <dbReference type="InterPro" id="IPR038902">
        <property type="entry name" value="INTS1"/>
    </dbReference>
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        <property type="entry name" value="INTS1_INTS2-bd"/>
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        <property type="entry name" value="INTS1_R4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022145">
        <property type="entry name" value="INTS1_RPB2-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21224">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21224:SF1">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22927">
        <property type="entry name" value="INT1_R3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22929">
        <property type="entry name" value="INTS1_INTS2-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22928">
        <property type="entry name" value="INTS1_R4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12432">
        <property type="entry name" value="INTS1_RP2B-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrator complex subunit 1 RPB2-binding" evidence="2">
        <location>
            <begin position="321"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="Integrator complex subunit 1 INTS2-binding" evidence="5">
        <location>
            <begin position="968"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="domain" description="Integrator complex subunit 1 R3" evidence="3">
        <location>
            <begin position="1861"/>
            <end position="2018"/>
        </location>
    </feature>
    <feature type="domain" description="Integrator complex subunit 1 R4" evidence="4">
        <location>
            <begin position="2075"/>
            <end position="2176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="919"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="921"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="935"/>
            <end position="944"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017310147.1"/>
        </source>
    </evidence>
    <sequence length="2214" mass="247353" checksum="F2270DB630FF35FF" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PWA7</accession>
    <name>A0A2D0PWA7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Wiskott-Aldrich syndrome protein family member</fullName>
            <shortName evidence="7">WASP family protein member</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">wasf2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Binds actin and the Arp2/3 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
            <location evidence="1 7">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SCAR/WAVE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017310329.1">
        <property type="nucleotide sequence ID" value="XM_017454840.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PWA7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029870"/>
    <dbReference type="GeneID" id="108257242"/>
    <dbReference type="KEGG" id="ipu:108257242"/>
    <dbReference type="CTD" id="10163"/>
    <dbReference type="OMA" id="PDMAYND"/>
    <dbReference type="OrthoDB" id="1060785at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031209">
        <property type="term" value="C:SCAR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034237">
        <property type="term" value="F:protein kinase A regulatory subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000601">
        <property type="term" value="P:positive regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000012">
        <property type="entry name" value="Wiskott-Aldrich syndrome protein family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.150">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028288">
        <property type="entry name" value="SCAR/WAVE_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12902:SF6">
        <property type="entry name" value="ACTIN-BINDING PROTEIN WASF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12902">
        <property type="entry name" value="WASP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00246">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH2" evidence="9">
        <location>
            <begin position="424"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="175"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="220"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="313"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="358"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017310329.1"/>
        </source>
    </evidence>
    <sequence length="486" mass="52961" checksum="E69EC05652E14399" modified="2017-12-20" version="1">MPLVMRNIEPRHLCRQTLPSTVKSELECVTNITLANIIRQLGSLSRFAEDVFGELFIQASLFADRVNTLGERVDKLQVKVTQLDPKEEEVSLQAITTRKAFRSNLIQDQQLFTRPSLPQPVQDTYQTCNEPPPLNILSIYRDDGKEALKFYTDPSYFFDLWKEQMLQDTKDIMKEKRKHRKEKRDNPNRGNLNPRKIKTRKEEWERRKMGEEFVVPKSDLGNSLDVVNGSIGSGDDMGTIDSEGFDQSTASYSYDPGSPLPPPVLDDDGFLPPPPPDMTYNDVPCATSPQRPASILSPAHPPPAPPTMSTSPPGRPSVAPPVAPPPPPPMGMAPPPPPLGFDAPPSPPPPAFSSISPSIPPPPPMDPSVPSPPPPPAGGGPPPPPPPPPPPGPPPSFAPSAPAPPPSGGPSSTPAPKPQPAPDGRSDLLAAIRQGFNLRKVEEQREQEKRDFVGNDVAAILSRRIAVECSDSEDDSSEFDDEEWSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PWY8</accession>
    <name>A0A2D0PWY8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transmembrane protein 121Ab</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">tmem121ab</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TMEM121 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017311018.1">
        <property type="nucleotide sequence ID" value="XM_017455529.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PWY8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030966"/>
    <dbReference type="GeneID" id="108257619"/>
    <dbReference type="KEGG" id="ipu:108257619"/>
    <dbReference type="CTD" id="569213"/>
    <dbReference type="OMA" id="VCHGHAR"/>
    <dbReference type="OrthoDB" id="9926693at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032776">
        <property type="entry name" value="CECR6/TMEM121"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042314">
        <property type="entry name" value="TMEM121"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31046">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31046:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14997">
        <property type="entry name" value="CECR6_TMEM121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017311018.1"/>
        </source>
    </evidence>
    <sequence length="316" mass="35843" checksum="328F5E5577EBB6EC" modified="2017-12-20" version="1">MVLPPPDKRHVCLTTIVIMTSMAFMDAYLVEQNQGPRKIGVCIIVLVGDVCFLIVLRYVAVWVGAEVRTARRGYAMILWFLYIFVLEIKLYFVFQNCKADRKSLETVARKALTLLLSVCVPGLYLVLVALDSMEYVRTFRKKEDMRGRLFWVALDLLDLLDIQANLWEPQRTGLPIWAEGLMFFYCYILLLILPCVSLSEISVQGENVSPQKMMLYPMLSLVTINVVTILIRGVNMVLFQDSRVSTIFVGKNVVAIATKVCTFLEYRKQSREFPARENATGIPMEIQQNSVGHGQALPNATSLPHEPTPVQEIIDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PXJ8</accession>
    <name>A0A2D0PXJ8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Left-right determination factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">lft1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017311223.1">
        <property type="nucleotide sequence ID" value="XM_017455734.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PXJ8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000011339"/>
    <dbReference type="GeneID" id="108257744"/>
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        <property type="term" value="F:transforming growth factor beta receptor binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:anterior/posterior axis specification"/>
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        <property type="entry name" value="TGF_beta_LEFTY1_2"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Cystine-knot cytokines"/>
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        <property type="entry name" value="LRDF"/>
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    </dbReference>
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        <property type="entry name" value="LEFT-RIGHT DETERMINATION FACTOR"/>
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    </dbReference>
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        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TGFb_propeptide"/>
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    </dbReference>
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        <property type="entry name" value="TGFb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01427">
        <property type="entry name" value="TGFBETA4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0202">Cytokine</keyword>
    <keyword evidence="6" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6 7" id="KW-0339">Growth factor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012293821" description="Left-right determination factor" evidence="8">
        <location>
            <begin position="21"/>
            <end position="358"/>
        </location>
    </feature>
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        <location>
            <begin position="249"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR037402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017311223.1"/>
        </source>
    </evidence>
    <sequence length="358" mass="40760" checksum="E69AC5C1673AF2E6" modified="2017-12-20" version="1">MTSVLASCFLCALILALTEAFTHDDMKDSLLKKLGLSEVPQIHKRDLENLVVPAHIKNKYVSMLKLHHERRRRSLPSLAGILRGIPGNADISGEFVYSDTTRQRVVFEMNSRIPDNSEVTMAELKLYKKASHKRSMPERKGQRPVNNARVSIYWVDMLGNGSNRTSLVDSRLIPIHEAGWKSFDVTQAIHYWSKSERNAPMHLEVWIEGERPGSYAAEMAKNVQFTTQEPSDILGKPELVLYTLNLEEFGSHGDCEPNKHKDVCCREEYFINFRALTWTQYWVIEPAGYQAFRCAGGCRQPQRGLYTYGQRKCAVVESSPLPMMYLVKKGDYTEIEVAEFPNMIVEECGCAMDNTSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PZK0</accession>
    <name>A0A2D0PZK0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Splicing factor 3B subunit 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pre-mRNA branch site protein p14</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">SF3b 14 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">sf3b6</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017310861.1">
        <property type="nucleotide sequence ID" value="XM_017455372.3"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A2D0PZK0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035494"/>
    <dbReference type="GeneID" id="108257514"/>
    <dbReference type="KEGG" id="ipu:108257514"/>
    <dbReference type="CTD" id="51639"/>
    <dbReference type="OMA" id="HQPDKMV"/>
    <dbReference type="OrthoDB" id="275748at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12241">
        <property type="entry name" value="RRM_SF3B14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000106">
        <property type="entry name" value="Splicing factor 3b, subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034150">
        <property type="entry name" value="SF3B6_RRM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="19"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00070534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00080799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00082208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017310861.1"/>
        </source>
    </evidence>
    <sequence length="125" mass="14585" checksum="A1439DACB78A3208" modified="2017-12-20" version="1">MAMQAAKRANIRLPPEVNRILYIRNLPYKITAEEMYDIFGKYGPIRQIRVGNTPETRGTAYVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYNANRAFQKMDTKKKEEQLKLLKEKYGINTDPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q2I3</accession>
    <name>A0A2D0Q2I3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Brain-specific angiogenesis inhibitor 1-associated protein 2-like protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">baiap2l2a</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017312497.1">
        <property type="nucleotide sequence ID" value="XM_017457008.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q2I3"/>
    <dbReference type="GeneID" id="108258393"/>
    <dbReference type="KEGG" id="ipu:108258393"/>
    <dbReference type="CTD" id="394210"/>
    <dbReference type="OrthoDB" id="9944156at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051764">
        <property type="term" value="P:actin crosslink formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030838">
        <property type="term" value="P:positive regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013606">
        <property type="entry name" value="I-BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027681">
        <property type="entry name" value="IRSp53/IRTKS/Pinkbar"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14206">
        <property type="entry name" value="BRAIN-SPECIFIC ANGIOGENESIS INHIBITOR 1-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14206:SF5">
        <property type="entry name" value="BRAIN-SPECIFIC ANGIOGENESIS INHIBITOR 1-ASSOCIATED PROTEIN 2-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08397">
        <property type="entry name" value="IMD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51338">
        <property type="entry name" value="IMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="IMD" evidence="5">
        <location>
            <begin position="1"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="262"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="230"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="345"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="358"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="372"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017312497.1"/>
        </source>
    </evidence>
    <sequence length="425" mass="47596" checksum="E661EC9494773211" modified="2017-12-20" version="1">MSGLNSDQLHRSTLGVYMTLMDQFNPSLQRLVALGNNYIQAFQALAATSEAYFSALGKMGEQAMQTMSSHLLGHILIQISESQRRLTSELAGVFRWFHNEVLQEMENNTRIDKDYIFDSRRRYEIEVRNQAMALERQLRRGGTQDVNEYVQFLRESQREALKEQERRYRFLAEKHCGLTQSIVYIMTKTGSTLQQTAEAWREQVNQTRGSRPGTASRLEDMVANDQGQVWAEQPLGRVPSRGPSPQPTRSRSNSFGETRGSGGGRMMRALVPHPPSSNSTLLPFSKGETITVLVPEPRNGWLYGCSESSARQGWFPAAYVGPMEDTPRLPDFGSGGLLRSSRSMSNLLDQSSSNRTNEPPPPPPPPPPPPSKSNSSRPVTPTSSENKKQDNYSSRPELFPRGTNPFATVKLKPTTTNDKSAPRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q4J4</accession>
    <name>A0A2D0Q4J4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">CMRF35-like molecule 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC108258799</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017313219.2">
        <property type="nucleotide sequence ID" value="XM_017457730.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q4J4"/>
    <dbReference type="GeneID" id="108258799"/>
    <dbReference type="OrthoDB" id="8920197at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05716">
        <property type="entry name" value="IgV_pIgR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF118">
        <property type="entry name" value="CMRF35-LIKE MOLECULE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5038070141" evidence="5">
        <location>
            <begin position="19"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="6">
        <location>
            <begin position="19"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="261"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="270"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017313219.2"/>
        </source>
    </evidence>
    <sequence length="293" mass="32364" checksum="CB92AB90879DC62B" modified="2023-02-22" version="2">MKILLIFTFCLITAGSDAVTTVTGYRGRSVQIKCPYGSGYEEYNKYLCRGKCPHGGNKDIPVHSGSPAEDTRFSLYDDTTAKVFTVTITDLRTEDGNTYWCVIERTGYDIYTELLLLVKTVDPAITTISHTTHTTHPASTHSMSPSVHVETTHSTTDYRSNVLLFPANPLSDANHLTNTHGKYNITCAICEILAKREREHTLNHHSTEKVSLGFVNALTTLLLCGLVSGMNYSEPYKQRTTGSQVAGTDASAGLRRSIDKARTQIQIDKQNSKQETGKGRSINKLDKARISEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q4Q5</accession>
    <name>A0A2D0Q4Q5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Small kinetochore-associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">knstrn</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4 5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017312339.1">
        <property type="nucleotide sequence ID" value="XM_017456850.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017312340.1">
        <property type="nucleotide sequence ID" value="XM_017456851.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q4Q5"/>
    <dbReference type="GeneID" id="108258303"/>
    <dbReference type="KEGG" id="ipu:108258303"/>
    <dbReference type="CTD" id="90417"/>
    <dbReference type="GeneTree" id="ENSGT00390000010376"/>
    <dbReference type="OMA" id="TGHRHES"/>
    <dbReference type="OrthoDB" id="9940269at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051988">
        <property type="term" value="P:regulation of attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007051">
        <property type="term" value="P:spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033373">
        <property type="entry name" value="SKAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31940">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SMALL KINETOCHORE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="60"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017312339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017312340.1"/>
        </source>
    </evidence>
    <sequence length="205" mass="23455" checksum="197F2DEF4E193227" modified="2017-12-20" version="1">MKRVDRAPVKDTAIPTHPNLASKKANVKTDPETMIRKNPAKSLKGPSVRYGLQSDVKEQNRLLLVANEDLLKQISELKENVSVLEQRCSDLQENNTEIKKQLRDCHVLLIEENLDPVSGEKVGEAVEQQAGQRKELMTISQKLLSELKLFDDFTKEHRTHLTEVQNTMRSLKGARETLHLERESFCTYAENVERALEDAERLLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q6R0</accession>
    <name>A0A2D0Q6R0_ICTPU</name>
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        <submittedName>
            <fullName evidence="17 18 19">Glucagon receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17 18 19 20" type="primary">gcgrb</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17 18 19" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17 18 19">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017313032.1">
        <property type="nucleotide sequence ID" value="XM_017457543.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017313033.1">
        <property type="nucleotide sequence ID" value="XM_017457544.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017313034.1">
        <property type="nucleotide sequence ID" value="XM_017457545.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053532215.1">
        <property type="nucleotide sequence ID" value="XM_053676240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q6R0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000014390"/>
    <dbReference type="GeneID" id="108258714"/>
    <dbReference type="KEGG" id="ipu:108258714"/>
    <dbReference type="CTD" id="565237"/>
    <dbReference type="GeneTree" id="ENSGT00940000165036"/>
    <dbReference type="OMA" id="CWERNMN"/>
    <dbReference type="OrthoDB" id="5967113at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016519">
        <property type="term" value="F:gastric inhibitory peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004967">
        <property type="term" value="F:glucagon receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017046">
        <property type="term" value="F:peptide hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15267">
        <property type="entry name" value="7tmB1_GCGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000133">
        <property type="entry name" value="Glucagon receptor a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050332">
        <property type="entry name" value="GPCR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001749">
        <property type="entry name" value="GPCR_2_GIP_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620:SF29">
        <property type="entry name" value="GLUCAGON RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620">
        <property type="entry name" value="PDF RECEPTOR-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02793">
        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01129">
        <property type="entry name" value="GIPRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111418">
        <property type="entry name" value="Hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00649">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17 18" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013510351" evidence="13">
        <location>
            <begin position="22"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="288"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="332"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="14">
        <location>
            <begin position="35"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="15">
        <location>
            <begin position="125"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="418"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="418"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017313032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017313033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017313034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_053532215.1"/>
        </source>
    </evidence>
    <sequence length="508" mass="58574" checksum="5358DF98C578552A" modified="2017-12-20" version="1">MSLRLLLLLLTFSSHTQVCQALKELREQWAIYRNECIQSFSSSPTATGLVCNRTFDHYTCWPDGSPGSIVNVSCPWYLPWYRKVKQGKVYRACGVDGRWADSKNTTECDENDDGLEEYWQIISKFRVMYTVGYSLSLAALVLALGILAAFRKLHCMRNSIHMNLFGSFILRAVSILAKDALLDLINTSQTERYRYQDQSSFDVKITVGCRVAMVMMQYSVIANNYWLLVEGLYLHSLLVITVFSERNYFYVYLCIGWGVPVIFVLPWVTLKYLYENDLCWEKNVNMGYWWIIRSPILFAYLINFFIFIRIIKILMSKLKGHQMRYTDYKFRLAKSTLTLIPLLGIHAILFTFVIDESVNRESLLRLVRLFYDLFFSSFQGLLVAILYCFVNKEVQNEMFKRWKRWKLGKDIEEEYRNTHSQMAHNKSASVNVASPPERSDTPEPAASAPEESCRLVISCRNGTGLAQGAGRSGLQEGTSNCSSLTEDICLEERVHNSDHRAGTKECHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q813</accession>
    <name>A0A2D0Q813_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">Zona pellucida sperm-binding protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC108259431</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Zona pellucida</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Cell membrane</location>
            <topology evidence="14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.</text>
    </comment>
    <comment type="PTM">
        <text evidence="14">Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the ZP domain family. ZPC subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017314439.2">
        <property type="nucleotide sequence ID" value="XM_017458950.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q813"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000023098"/>
    <dbReference type="GeneID" id="108259431"/>
    <dbReference type="KEGG" id="ipu:108259431"/>
    <dbReference type="OrthoDB" id="8880842at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035805">
        <property type="term" value="C:egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032190">
        <property type="term" value="F:acrosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035804">
        <property type="term" value="F:structural constituent of egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035803">
        <property type="term" value="P:egg coat formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000344">
        <property type="term" value="P:positive regulation of acrosome reaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.3210:FF:000001">
        <property type="entry name" value="Zona pellucida sperm-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.4100:FF:000002">
        <property type="entry name" value="Zona pellucida sperm-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055356">
        <property type="entry name" value="ZP-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048290">
        <property type="entry name" value="ZP_chr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576:SF2">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23344">
        <property type="entry name" value="ZP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00023">
        <property type="entry name" value="ZPELLUCIDA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 14" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="12 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 14" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 14" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039962906" description="Zona pellucida sperm-binding protein 3" evidence="14">
        <location>
            <begin position="22"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="15">
        <location>
            <begin position="125"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017314439.2"/>
        </source>
    </evidence>
    <sequence length="435" mass="47121" checksum="FBEF78EE4596E0E2" modified="2023-06-28" version="2">MGFNYVGFGALLILTVGLSAAQWPGLMNAPAGFQPNKPSHPSLLVSAGLPSQWMQSDNQSGSPAVAPFGLQLQNPSDAQDQQVMQRPVKKLTWCFPAAPQIPTPPTPVNFVRQSDVVPTQSVTARCNETAVYVEVRKDLFGPRASLNITALTLGGCAAKGVAVSSQVLVYESPLHGCNSKLTVTANELVYIFTLGIASVPLISAPILRSPGAMAFVECHYPRFHNVSSSALLPAWIPYASAQAAEEHLLFSLRLMMDDWISERPSDYNYQLGELINIEASVVQFNHVPLRVFVDSCVATAVPDINAVPRYSFIENHGCLIDSKLTHSSSHFMPQTQAAKLGFQLEAFKFQHVNSSWFYIACILKATAASAPADAEHKACSFSGNRWTAAYGVDQVCSCCNSSCGSRKGRDLSSEQGLQLEREVSLGPIMVLENVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QDI9</accession>
    <name>A0A2D0QDI9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 4C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">eif1axa</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 10">Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon. This protein enhances formation of the cap-proximal complex. Together with EIF1, facilitates scanning, start codon recognition, promotion of the assembly of 48S complex at the initiation codon (43S PIC becomes 48S PIC after the start codon is reached), and dissociation of aberrant complexes. After start codon location, together with EIF5B orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex. Is released after 80S initiation complex formation, just after GTP hydrolysis by EIF5B, and before release of EIF5B. Its globular part is located in the A site of the 40S ribosomal subunit. Its interaction with EIF5 during scanning contribute to the maintenance of EIF1 within the open 43S PIC. In contrast to yeast orthologs, does not bind EIF1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the 43S pre-initiation complex (43S PIC), which is composed of the 40S ribosomal subunit, EIF1, eIF1A (EIF1AX), eIF3 complex, EIF5 and eIF2-GTP-initiator tRNA complex (eIF2 ternary complex). Interacts with EIF5; this interaction contributes to the maintenance of EIF1 within the open 43S PIC. Interacts through its C-terminal domain (CTD) with the CTD of EIF5B; from the location of the start codon by the 43S complex until the formation of the 80S complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the eIF-1A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017315781.1">
        <property type="nucleotide sequence ID" value="XM_017460292.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QDI9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000031589"/>
    <dbReference type="GeneID" id="108260200"/>
    <dbReference type="KEGG" id="ipu:108260200"/>
    <dbReference type="CTD" id="450062"/>
    <dbReference type="OMA" id="KMEDQEY"/>
    <dbReference type="OrthoDB" id="274995at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05793">
        <property type="entry name" value="S1_IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000071">
        <property type="entry name" value="Eukaryotic translation initiation factor 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00216">
        <property type="entry name" value="aIF_1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001253">
        <property type="entry name" value="TIF_eIF-1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018104">
        <property type="entry name" value="TIF_eIF-1A_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00523">
        <property type="entry name" value="eIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21668">
        <property type="entry name" value="EIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00652">
        <property type="entry name" value="eIF1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01262">
        <property type="entry name" value="IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8 10" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4 8 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="S1-like" evidence="12">
        <location>
            <begin position="22"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="115"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017315781.1"/>
        </source>
    </evidence>
    <sequence length="144" mass="16474" checksum="1C508B1549A32FD4" modified="2017-12-20" version="1">MPKNKGKGGKNRRRGKNENESEKRELVFKEDGQEYAQVIKMLGNGRLEAMCFDGVKRLCHIRGKLRKKVWINTSDIILIGLRDYQDNKADVILKYNADEARSLKAYGELPEHAKINETDTFGPGDDDEIQFDDIGDDDEDIDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QI09</accession>
    <name>A0A2D0QI09_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">E3 ubiquitin-protein ligase TRIM39</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">btr12</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017317140.1">
        <property type="nucleotide sequence ID" value="XM_017461651.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QI09"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013443"/>
    <dbReference type="GeneID" id="108260923"/>
    <dbReference type="KEGG" id="ipu:108260923"/>
    <dbReference type="CTD" id="767681"/>
    <dbReference type="OMA" id="CICSACA"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19802">
        <property type="entry name" value="Bbox1_TRIM8-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13733">
        <property type="entry name" value="SPRY_PRY_C-I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF49">
        <property type="entry name" value="BLOODTHIRSTY-RELATED GENE FAMILY, MEMBER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="18"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="9">
        <location>
            <begin position="164"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="10">
        <location>
            <begin position="363"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="399"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017317140.1"/>
        </source>
    </evidence>
    <sequence length="561" mass="63346" checksum="B3ACD076AA935F6C" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QL57</accession>
    <name>A0A2D0QL57_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">CD276 antigen</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC108263079</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017319009.1">
        <property type="nucleotide sequence ID" value="XM_017463520.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QL57"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000004523"/>
    <dbReference type="GeneID" id="108263079"/>
    <dbReference type="KEGG" id="ipu:108263079"/>
    <dbReference type="OMA" id="SENGGHE"/>
    <dbReference type="OrthoDB" id="5857426at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042130">
        <property type="term" value="P:negative regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042102">
        <property type="term" value="P:positive regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031295">
        <property type="term" value="P:T cell costimulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000142">
        <property type="entry name" value="V-set domain-containing T-cell activation inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053896">
        <property type="entry name" value="BTN3A2-like_Ig-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051713">
        <property type="entry name" value="T-cell_Activation_Regulation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466:SF14">
        <property type="entry name" value="BUTYROPHILIN SUBFAMILY 2 MEMBER A2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466">
        <property type="entry name" value="T-LYMPHOCYTE ACTIVATION ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22705">
        <property type="entry name" value="C2-set_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012045060" evidence="13">
        <location>
            <begin position="27"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="44"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="292"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="295"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="314"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017319009.1"/>
        </source>
    </evidence>
    <sequence length="326" mass="36679" checksum="1CDFCBC03EAB30A7" modified="2017-12-20" version="1">MSAHTGTPSWVCCGVHFLLLLAQMSCNMEFEISVPARVQMGMYGESVVLPCTFPVSSSWDAGSSVITWQRQLEVVHSFFYGLDQPQYQSQRYANRTSLFYQEMKNGNASLRLDRTTLEDAGEYTCSISTQLGSQRKSFGLKVAAFYPEPHVHIIMLNDGHVEVVVTSEGGYPSPSLQWLMGNESDITNDTLTQLRQDEHTRLYSVNSKLNLSGTVNSSITFIMKNPDLGQEIRRNIELFSENGGHEHEQNTATPFLLGLATGMLFILIVLIVIIFMCKMSFKKRSSRRGVGSEIQHSESRPCSDHQLQRSTCLSEEREHIAEPHSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QNE9</accession>
    <name>A0A2D0QNE9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein FAM13A isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">fam13a</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM13 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017318985.1">
        <property type="nucleotide sequence ID" value="XM_017463496.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QNE9"/>
    <dbReference type="GeneID" id="108263066"/>
    <dbReference type="CTD" id="10144"/>
    <dbReference type="OrthoDB" id="185175at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039102">
        <property type="entry name" value="FAM13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059029">
        <property type="entry name" value="FAM13A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15904">
        <property type="entry name" value="FAM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15904:SF18">
        <property type="entry name" value="PROTEIN FAM13A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26116">
        <property type="entry name" value="FAM13A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="52"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="368"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="441"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="763"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="701"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="974"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="368"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="471"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="491"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="507"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="527"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="548"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="773"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="786"/>
            <end position="795"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017318985.1"/>
        </source>
    </evidence>
    <sequence length="1055" mass="119524" checksum="9B9D6AD7CD62611B" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QPM0</accession>
    <name>A0A2D0QPM0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Anthrax toxin receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">antxr1d</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">SDA103</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton and thereby plays an important role in normal extracellular matrix (ECM) homeostasis. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells. May also act as a receptor for PLAU. Upon ligand binding, stimulates the phosphorylation of EGFR and ERK1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with gelatin and type 1 collagen. Interacts with the actin cytoskeleton.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cell projection</location>
            <location evidence="14">Filopodium membrane</location>
            <topology evidence="14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Cell projection</location>
            <location evidence="13">Lamellipodium membrane</location>
            <topology evidence="13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 17">Belongs to the ATR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017319405.1">
        <property type="nucleotide sequence ID" value="XM_017463916.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QPM0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000389"/>
    <dbReference type="GeneID" id="108263270"/>
    <dbReference type="KEGG" id="ipu:108263270"/>
    <dbReference type="CTD" id="563132"/>
    <dbReference type="OMA" id="LFRRGYN"/>
    <dbReference type="OrthoDB" id="10035766at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031527">
        <property type="term" value="C:filopodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031258">
        <property type="term" value="C:lamellipodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01474">
        <property type="entry name" value="vWA_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000017">
        <property type="entry name" value="Anthrax toxin receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017360">
        <property type="entry name" value="Anthrax_toxin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008399">
        <property type="entry name" value="Anthrax_toxin_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008400">
        <property type="entry name" value="Anthrax_toxin_rcpt_extracel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16059">
        <property type="entry name" value="ANTHRAX TOXIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16059:SF16">
        <property type="entry name" value="ANTHRAX TOXIN RECEPTOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05586">
        <property type="entry name" value="Ant_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05587">
        <property type="entry name" value="Anth_Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038023">
        <property type="entry name" value="Anthrax_toxin_receptor_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9 19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 17 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 17 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10 17 25" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 22" id="KW-0732">Signal</keyword>
    <keyword evidence="4 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012067616" description="Anthrax toxin receptor" evidence="22">
        <location>
            <begin position="30"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="23">
        <location>
            <begin position="43"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="396"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="466"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="396"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="429"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="492"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="517"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="38"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00061894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR038023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR038023-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR038023-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_017319405.1"/>
        </source>
    </evidence>
    <sequence length="558" mass="61746" checksum="B9BE883F7A4CE494" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QSP7</accession>
    <name>A0A2D0QSP7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">18S rRNA aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="6">2.5.1.157</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">tsr3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Aminocarboxypropyltransferase that catalyzes the aminocarboxypropyl transfer on pseudouridine at position 1248 (Psi1248) in 18S rRNA. It constitutes the last step in biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N(1)-methylpseudouridine(1248) in human 18S rRNA + S-adenosyl-L-methionine = N(1)-methyl-N(3)-[(3S)-3-amino-3-carboxypropyl]pseudouridine(1248) in human 18S rRNA + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63292"/>
            <dbReference type="Rhea" id="RHEA-COMP:11639"/>
            <dbReference type="Rhea" id="RHEA-COMP:16308"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
            <dbReference type="ChEBI" id="CHEBI:146234"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>an N(1)-methylpseudouridine in rRNA + S-adenosyl-L-methionine = N(1)-methyl-N(3)-[(3S)-3-amino-3-carboxypropyl]pseudouridine in rRNA + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63296"/>
            <dbReference type="Rhea" id="RHEA-COMP:11634"/>
            <dbReference type="Rhea" id="RHEA-COMP:16310"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
            <dbReference type="ChEBI" id="CHEBI:146234"/>
            <dbReference type="EC" id="2.5.1.157"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TDD superfamily. TSR3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.157" evidence="6"/>
    <dbReference type="RefSeq" id="XP_017321483.1">
        <property type="nucleotide sequence ID" value="XM_017465994.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QSP7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000426"/>
    <dbReference type="GeneID" id="108264448"/>
    <dbReference type="KEGG" id="ipu:108264448"/>
    <dbReference type="CTD" id="115939"/>
    <dbReference type="OMA" id="MVGTHPR"/>
    <dbReference type="OrthoDB" id="10262062at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106388">
        <property type="term" value="F:rRNA small subunit aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01116">
        <property type="entry name" value="TSR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022968">
        <property type="entry name" value="Tsr3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007177">
        <property type="entry name" value="Tsr3_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002621">
        <property type="entry name" value="PRK02287.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426:SF0">
        <property type="entry name" value="18S RRNA AMINOCARBOXYPROPYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN TSR3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="Fer4_RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04034">
        <property type="entry name" value="Ribo_biogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNase L inhibitor RLI-like possible metal-binding" evidence="9">
        <location>
            <begin position="63"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="16S/18S rRNA aminocarboxypropyltransferase Tsr3 C-terminal" evidence="8">
        <location>
            <begin position="100"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="224"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="231"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="256"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017321483.1"/>
        </source>
    </evidence>
    <sequence length="298" mass="33700" checksum="953C4A8B9BA292BD" modified="2017-12-20" version="1">MGRKKPFQSERQTSRGKDKRHKMKGKSLETFTEEMQEFLEAQCKSTESEDAAAAAASAKFPCPLAMWELGHCDPKRCTGRKLARKGLVRCLRLNQRFNGLVLSPMGVKYVTPADREIVAEGGLAVIDCSWARLEDTPFSKMIGSHPRLLPYLVAANPVNYGKPCKLSCVEAYAATFCIVGFQDLAVLLLRKFKWGLGFLELNKTLLQRYATCKSEEELLTVEKEYLTSSPSEEEELDPFDVDSGRECMNLNRPLREEEDDTESSEAETSEEDDEQEEEDEKDAAEDEKKDKENIEDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QVM8</accession>
    <name>A0A2D0QVM8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Major facilitator superfamily domain-containing protein 12 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mfsd12b</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017322473.1">
        <property type="nucleotide sequence ID" value="XM_017466984.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QVM8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020373"/>
    <dbReference type="GeneID" id="108264970"/>
    <dbReference type="KEGG" id="ipu:108264970"/>
    <dbReference type="CTD" id="564425"/>
    <dbReference type="OMA" id="EITIFRY"/>
    <dbReference type="OrthoDB" id="1730117at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043474">
        <property type="term" value="P:pigment metabolic process involved in pigmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048021">
        <property type="term" value="P:regulation of melanin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17491">
        <property type="entry name" value="MFS_MFSD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000905">
        <property type="entry name" value="Major facilitator superfamily domain containing 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039672">
        <property type="entry name" value="MFS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11328">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11328:SF28">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13347">
        <property type="entry name" value="MFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="295"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="325"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="349"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="385"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="430"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017322473.1"/>
        </source>
    </evidence>
    <sequence length="457" mass="50702" checksum="1442A88DD69F5A53" modified="2017-12-20" version="1">MSEGARTLPAFRRFTYSVGHVLNDLCASMWFTYLLLYYQSVLSFRDSSAGLLLLFGQIADGIATPLVGYESDRTRGCGTYGKRKSWHLVGTICVLLSFPFIFNPCVGCGEVTPQWVGLTYFTPFIIIFQFGWASTQISHLSLIPELVSCEHAKVELTAYRYAFTVLSNITVYAVAWLLLRFQHPADPSVADQLSHLDVPVFRDLALIVLGIGAVFSLIFHLGTRERTRREADESRPLLTRSGENSAPLLQWKHWLTEPSFYQVAALYMCTRLIVNLSQTYISMYLINSLFLPKNYIATIPLVLYVSGFVSSLAMKPISKRLGVSMTYLAGLVPIMAFGIWVLLDLKMGVRVYGAAVLLGAGSAVILVMSLSMTANLIGDQTQSGAFVYGAMSFTDKVANGLAVMIIQSLYPCQTLVCCSDCVWFYHRVMVAVTGGVSLITAFCLFSLVVWPIKIRRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QX88</accession>
    <name>A0A2D0QX88_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13 14 15">Zinc finger protein Xfin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13 14 15" type="primary">si:dkeyp-84f3.5</name>
    </gene>
    <organism evidence="12 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13 14 15" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13 14 15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017323004.1">
        <property type="nucleotide sequence ID" value="XM_017467515.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017323014.1">
        <property type="nucleotide sequence ID" value="XM_017467525.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017323023.1">
        <property type="nucleotide sequence ID" value="XM_017467534.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QX88"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003640"/>
    <dbReference type="GeneID" id="108265322"/>
    <dbReference type="KEGG" id="ipu:108265322"/>
    <dbReference type="GeneTree" id="ENSGT00940000166443"/>
    <dbReference type="OMA" id="CTETFSC"/>
    <dbReference type="OrthoDB" id="6359816at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050636">
        <property type="entry name" value="C2H2-ZF_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772:SF13">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772">
        <property type="entry name" value="ZINC FINGER PROTEIN 200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="112"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="214"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="277"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="360"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="387"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="479"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="510"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="582"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="240"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="418"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="531"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="241"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="418"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="531"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="562"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017323004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017323014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017323023.1"/>
        </source>
    </evidence>
    <sequence length="605" mass="69487" checksum="94ACB5DC8C1F61E1" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QY70</accession>
    <name>A0A2D0QY70_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Apolipoprotein C-I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">apoc1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the apolipoprotein C1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017323353.1">
        <property type="nucleotide sequence ID" value="XM_017467864.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QY70"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000004235"/>
    <dbReference type="GeneID" id="108265490"/>
    <dbReference type="KEGG" id="ipu:108265490"/>
    <dbReference type="CTD" id="341"/>
    <dbReference type="OMA" id="GTSTRNW"/>
    <dbReference type="OrthoDB" id="8941712at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034364">
        <property type="term" value="C:high-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034361">
        <property type="term" value="C:very-low-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004859">
        <property type="term" value="F:phospholipase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042157">
        <property type="term" value="P:lipoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032375">
        <property type="term" value="P:negative regulation of cholesterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050995">
        <property type="term" value="P:negative regulation of lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010916">
        <property type="term" value="P:negative regulation of very-low-density lipoprotein particle clearance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034447">
        <property type="term" value="P:very-low-density lipoprotein particle clearance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.30">
        <property type="entry name" value="Apolipoprotein C-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043081">
        <property type="entry name" value="ApoC-1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006781">
        <property type="entry name" value="ApoC-I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16565">
        <property type="entry name" value="APOLIPOPROTEIN C-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16565:SF2">
        <property type="entry name" value="APOLIPOPROTEIN C-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04691">
        <property type="entry name" value="ApoC-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012180870" evidence="7">
        <location>
            <begin position="21"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017323353.1"/>
        </source>
    </evidence>
    <sequence length="87" mass="10133" checksum="D93861926DFA5857" modified="2017-12-20" version="1">MKLYLAVATLMLVLIAHSEAQEESTIEQQFANFQARFKEFGDDVTEKTKNALKQIENSEIVSKTKDWLTEQLEKVRQKFDEVINKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QYY8</accession>
    <name>A0A2D0QYY8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Chemokine-like protein TAFA-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tafa3b</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TAFA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017322719.1">
        <property type="nucleotide sequence ID" value="XM_017467230.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QYY8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006783"/>
    <dbReference type="GeneID" id="108265113"/>
    <dbReference type="KEGG" id="ipu:108265113"/>
    <dbReference type="CTD" id="100003386"/>
    <dbReference type="OrthoDB" id="9924724at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048018">
        <property type="term" value="F:receptor ligand activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020350">
        <property type="entry name" value="Chemokine-like_TAFA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051743">
        <property type="entry name" value="TAFA_chemokine-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31770">
        <property type="entry name" value="CHEMOKINE-LIKE PROTEIN TAFA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31770:SF3">
        <property type="entry name" value="CHEMOKINE-LIKE PROTEIN TAFA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12020">
        <property type="entry name" value="TAFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017322719.1"/>
        </source>
    </evidence>
    <sequence length="132" mass="14507" checksum="B1B405A040E6796F" modified="2017-12-20" version="1">MRERDVQSVCAWLLLLTLALLSLWGRLALASSHRNHIVHVKAGTCEVIAAHRCCNKNKIEERSQTVKCSCLPGQVAGTTRAAPSCVDASIVAQKWWCQMQPCLDGEECKVLPDLTGWSCSTGNKVKTTKVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R6N1</accession>
    <name>A0A2D0R6N1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Neuromedin-S</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">nms</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017326239.1">
        <property type="nucleotide sequence ID" value="XM_017470750.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R6N1"/>
    <dbReference type="GeneID" id="108266918"/>
    <dbReference type="KEGG" id="ipu:108266918"/>
    <dbReference type="CTD" id="129521"/>
    <dbReference type="OrthoDB" id="9940794at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043253">
        <property type="entry name" value="NmS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32414">
        <property type="entry name" value="NEUROMEDIN-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32414:SF2">
        <property type="entry name" value="NEUROMEDIN-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012248893" evidence="1">
        <location>
            <begin position="29"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017326239.1"/>
        </source>
    </evidence>
    <sequence length="138" mass="15868" checksum="D90C5B2909225256" modified="2017-12-20" version="1">MRETCFRELYSLCLILWCLTGFYSQAECYPLLDCGDDGDYTQGSAVQSSLCGLVWGDQKKEQIQNVFKRFLFHYSKARNSVGTVERESHSVHPLMRLSPKFTLRRKKQQVLSTLKKTCGRSDRFIRGIRELTGCAGGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R7J7</accession>
    <name>A0A2D0R7J7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13">Shugoshin 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">sgo2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12 13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the shugoshin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017326026.2">
        <property type="nucleotide sequence ID" value="XM_017470537.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017326027.2">
        <property type="nucleotide sequence ID" value="XM_017470538.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R7J7"/>
    <dbReference type="GeneID" id="108266797"/>
    <dbReference type="KEGG" id="ipu:108266797"/>
    <dbReference type="CTD" id="151246"/>
    <dbReference type="OrthoDB" id="5990092at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045132">
        <property type="term" value="P:meiotic chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051177">
        <property type="term" value="P:meiotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038889">
        <property type="entry name" value="Shugoshin1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011515">
        <property type="entry name" value="Shugoshin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21577">
        <property type="entry name" value="SHUGOSHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21577:SF3">
        <property type="entry name" value="SHUGOSHIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07557">
        <property type="entry name" value="Shugoshin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-0137">Centromere</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Shugoshin C-terminal" evidence="10">
        <location>
            <begin position="835"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="116"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="274"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="573"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="141"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="173"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="274"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="295"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="573"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017326026.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017326027.2"/>
        </source>
    </evidence>
    <sequence length="884" mass="99976" checksum="DC3BCF7F388BB578" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R7T7</accession>
    <name>A0A2D0R7T7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Interferon alpha/beta receptor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC108266841</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017326116.1">
        <property type="nucleotide sequence ID" value="XM_017470627.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R7T7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006505"/>
    <dbReference type="GeneID" id="108266841"/>
    <dbReference type="KEGG" id="ipu:108266841"/>
    <dbReference type="OMA" id="PCSNIAL"/>
    <dbReference type="OrthoDB" id="10031784at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015373">
        <property type="entry name" value="Interferon/interleukin_rcp_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050650">
        <property type="entry name" value="Type-II_Cytokine-TF_Rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20859">
        <property type="entry name" value="INTERFERON/INTERLEUKIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20859:SF53">
        <property type="entry name" value="INTERLEUKIN-22 RECEPTOR SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09294">
        <property type="entry name" value="Interfer-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01108">
        <property type="entry name" value="Tissue_fac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013288368" evidence="2">
        <location>
            <begin position="31"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="31"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="131"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017326116.1"/>
        </source>
    </evidence>
    <sequence length="283" mass="31787" checksum="1C0BD8B6D2BC4E82" modified="2017-12-20" version="1">MRSTKMNCTRQHGILIVWTIFFFLILEVHCSLTSPQNVSIVSFNLEHKLTWTPGPGTAAVTHFRVQSYNQKRKLWISVKSCSDLQIGESCDLTTTFNETFGHYQARVQAFSQDQESNWTTSKFFTPLVDTTLGPPLVSLTGCGNCLLLKLSPPLSVEQKHDPLTYLYQRYTVNVSRTRDKAQFVMKASNGENLINYLEPGVEYCITAIAVTSFKNLVLPSDPQCTYTSSQPLNTVAMFLSALCAVFLLVLLLCAGLIYTGQFGNFHTTLLRALVSFLPFLERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R8A5</accession>
    <name>A0A2D0R8A5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fibronectin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">fn1a</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017326060.1">
        <property type="nucleotide sequence ID" value="XM_017470571.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000016032"/>
    <dbReference type="GeneID" id="108266812"/>
    <dbReference type="KEGG" id="ipu:108266812"/>
    <dbReference type="CTD" id="100005469"/>
    <dbReference type="OMA" id="GHCITDS"/>
    <dbReference type="OrthoDB" id="261433at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043394">
        <property type="term" value="F:proteoglycan binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006953">
        <property type="term" value="P:acute-phase response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007044">
        <property type="term" value="P:cell-substrate junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00061">
        <property type="entry name" value="FN1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="CDD" id="cd00062">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000004">
        <property type="entry name" value="Fibronectin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000006">
        <property type="entry name" value="Fibronectin 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000007">
        <property type="entry name" value="Fibronectin 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000018">
        <property type="entry name" value="Fibronectin 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000099">
        <property type="entry name" value="Fibronectin 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.10.10:FF:000001">
        <property type="entry name" value="Fibronectin 1a isoform 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000021">
        <property type="entry name" value="fibronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000227">
        <property type="entry name" value="Fibronectin isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000336">
        <property type="entry name" value="fibronectin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.10.10">
        <property type="entry name" value="Fibronectin, type II, collagen-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050991">
        <property type="entry name" value="ECM_Regulatory_Proteins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000083">
        <property type="entry name" value="Fibronectin_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000562">
        <property type="entry name" value="FN_type2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036943">
        <property type="entry name" value="FN_type2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708:SF4">
        <property type="entry name" value="FIBRONECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708">
        <property type="entry name" value="TENASCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00039">
        <property type="entry name" value="fn1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00040">
        <property type="entry name" value="fn2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00013">
        <property type="entry name" value="FNTYPEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00058">
        <property type="entry name" value="FN1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00059">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01253">
        <property type="entry name" value="FN1_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51091">
        <property type="entry name" value="FN1_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00023">
        <property type="entry name" value="FN2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51092">
        <property type="entry name" value="FN2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0011">Acute phase</keyword>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="6" id="KW-0133">Cell shape</keyword>
    <keyword evidence="7 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012903703" description="Fibronectin" evidence="10">
        <location>
            <begin position="22"/>
            <end position="2483"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-I" evidence="12">
        <location>
            <begin position="44"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-I" evidence="12">
        <location>
            <begin position="88"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-I" evidence="12">
        <location>
            <begin position="132"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-I" evidence="12">
        <location>
            <begin position="177"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-I" evidence="12">
        <location>
            <begin position="222"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-II" evidence="13">
        <location>
            <begin position="351"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-II" evidence="13">
        <location>
            <begin position="411"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-I" evidence="12">
        <location>
            <begin position="464"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-I" evidence="12">
        <location>
            <begin position="513"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-I" evidence="12">
        <location>
            <begin position="556"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="607"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="710"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="809"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="907"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="998"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="1087"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="1174"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="1269"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="1358"/>
            <end position="1452"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="1453"/>
            <end position="1540"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="1541"/>
            <end position="1631"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="1635"/>
            <end position="1725"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="1726"/>
            <end position="1814"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="1815"/>
            <end position="1905"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="1908"/>
            <end position="1998"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="2001"/>
            <end position="2092"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-I" evidence="12">
        <location>
            <begin position="2300"/>
            <end position="2344"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-I" evidence="12">
        <location>
            <begin position="2345"/>
            <end position="2387"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-I" evidence="12">
        <location>
            <begin position="2389"/>
            <end position="2429"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="356"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="370"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="416"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="430"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017326060.1"/>
        </source>
    </evidence>
    <sequence length="2483" mass="272487" checksum="6128F71917A163F4" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RBV1</accession>
    <name>A0A2D0RBV1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ribonuclease kappa-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rnaseka</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">SDA103</strain>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <tissue evidence="8">Blood</tissue>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="match status" value="1"/>
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            <end position="35"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="88"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017327616.1"/>
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    </evidence>
    <sequence length="101" mass="11356" checksum="A20B804584E1B1FC" modified="2017-12-20" version="1">MPSFILCGPKMAVCMLVLSMWGVIMLAMLGIFFSVHSAVLIEDVPLKEEDHQDSQNPLQNVYAVYNQVGYNCFIAAGIYVVIGLVSFVQVRLNKRNDYLVR</sequence>
</entry>
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            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
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                <person name="Yuan Z."/>
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                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
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                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
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                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
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                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="9">Blood</tissue>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
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    </comment>
    <dbReference type="RefSeq" id="XP_017328376.2">
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    <dbReference type="STRING" id="7998.ENSIPUP00000006311"/>
    <dbReference type="GeneID" id="108268138"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Doublecortin domain"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Doublecortin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036572">
        <property type="entry name" value="Doublecortin_dom_sf"/>
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    <dbReference type="PANTHER" id="PTHR23005:SF4">
        <property type="entry name" value="OXYGEN-REGULATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23005">
        <property type="entry name" value="RETINITIS PIGMENTOSA 1 PROTEIN"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="DCX"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Doublecortin (DC)"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="DC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="34"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Doublecortin" evidence="7">
        <location>
            <begin position="168"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
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            <end position="481"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="6">
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    <feature type="region of interest" description="Disordered" evidence="6">
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    <feature type="region of interest" description="Disordered" evidence="6">
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        <location>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50309"/>
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        <source>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
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            <taxon>Teleostei</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FAM234 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017330698.2">
        <property type="nucleotide sequence ID" value="XM_017475209.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017330777.2">
        <property type="nucleotide sequence ID" value="XM_017475288.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RJL0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006371"/>
    <dbReference type="GeneID" id="108269345"/>
    <dbReference type="KEGG" id="ipu:108269345"/>
    <dbReference type="CTD" id="83986"/>
    <dbReference type="OrthoDB" id="6364780at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055409">
        <property type="entry name" value="Beta-prop_FAM234A_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045232">
        <property type="entry name" value="FAM234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21419">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21419:SF7">
        <property type="entry name" value="PROTEIN FAM234A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23727">
        <property type="entry name" value="Beta-prop_FAM234A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="FAM234A/B beta-propeller" evidence="8">
        <location>
            <begin position="76"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017330698.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017330777.2"/>
        </source>
    </evidence>
    <sequence length="561" mass="61058" checksum="2EE875BE48168F92" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RQQ1</accession>
    <name>A0A2D0RQQ1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Iron-sulfur cluster transfer protein NUBPL</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Nucleotide-binding protein-like</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">nubpl</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Iron-sulfur cluster transfer protein involved in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). May deliver one or more Fe-S clusters to complex I subunits.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017332506.1">
        <property type="nucleotide sequence ID" value="XM_017477017.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RQQ1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030496"/>
    <dbReference type="GeneID" id="108270385"/>
    <dbReference type="KEGG" id="ipu:108270385"/>
    <dbReference type="CTD" id="80224"/>
    <dbReference type="OMA" id="CNHESHI"/>
    <dbReference type="OrthoDB" id="1741334at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000709">
        <property type="entry name" value="Iron-sulfur protein NUBPL isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044304">
        <property type="entry name" value="NUBPL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961:SF2">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017332506.1"/>
        </source>
    </evidence>
    <sequence length="328" mass="35731" checksum="DF352616AFC0D52E" modified="2017-12-20" version="1">MLFNQSCRKLSEVWKWNSASIVLWSCSNVRHPQPLSAGCKHDVRLKSSASGERQQERQRAHMARGLPKQKPIAGVKEVIVVASGKGGVGKSTTAVNLALGLMANDRSKTVGLLDADVYGPSVPKLMNLRGNPELTDNNLMRPLMNFGIPCMSMGFLVEDTAPIVWRGLMVMSAIEKLLRQVDWGSLDYLVIDMPPGTGDVQLSITQNIPIAGAVIVSTPQDIALLDARRGAEMFRKVNVPVLGLVQNMSVFQCPQCNHQTHIFGSDGARELASALGVEVLGDIPLHLNIRETSDKGQPVVVSSPDSPEAEAYRRVALAVVRRLREENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RTF3</accession>
    <name>A0A2D0RTF3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Paralemmin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Paralemmin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">palm1a</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">SDA103</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="20">Interacts with dopamine receptor DRD3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Apicolateral cell membrane</location>
            <topology evidence="18">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Basolateral cell membrane</location>
            <topology evidence="19">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Filopodium membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the paralemmin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017333240.1">
        <property type="nucleotide sequence ID" value="XM_017477751.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RTF3"/>
    <dbReference type="GeneID" id="108270811"/>
    <dbReference type="KEGG" id="ipu:108270811"/>
    <dbReference type="CTD" id="550557"/>
    <dbReference type="OrthoDB" id="8964272at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016327">
        <property type="term" value="C:apicolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031527">
        <property type="term" value="C:filopodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004965">
        <property type="entry name" value="Paralemmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10498:SF6">
        <property type="entry name" value="PARALEMMIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10498">
        <property type="entry name" value="PARALEMMIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03285">
        <property type="entry name" value="Paralemmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0133">Cell shape</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0488">Methylation</keyword>
    <keyword evidence="14" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0636">Prenylation</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0770">Synapse</keyword>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="16"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="324"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="97"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="328"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00037796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00037871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00038823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00040790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00041963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_017333240.1"/>
        </source>
    </evidence>
    <sequence length="382" mass="41903" checksum="B3A935848E8523E2" modified="2017-12-20" version="1">MSEALSQQERLQAIAEKRKKQTEIENKKRQLEDDRRQLQHLKSKALRERWLLDGAPSAGSEEDEAKKQLQEDEAKTKGLEEAILRLEQELEELETGVSATSTKENLSDAAQQEESKTVDNMVMGDSIKEVREIKVHLSPRLEKSRGDSDMMRAAMYSVEITVEKDLVTGETKVLSTNTLLPKDFSHQGVKVYEDEQKVVHEVRGTDGPLTNGIHQLSSSEVEELIHKADEASVSDGSKATPQRAVEEAAKLEAGTEQVGVTPVKEITGVEAKPSPAVPQTDVNDASAEKPVTMVFMGYQDVEDEDETKKVLGLESTTVKAELVVIEDGENKNGAKEDQAPPNGGPAEPAKAQEEKGEEGDPEAGAAEMKSKEKQPCKCCTIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S1D6</accession>
    <name>A0A2D0S1D6_ICTPU</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC108272377 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108272377</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017336251.1">
        <property type="nucleotide sequence ID" value="XM_017480762.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S1D6"/>
    <dbReference type="GeneID" id="108272377"/>
    <dbReference type="KEGG" id="ipu:108272377"/>
    <dbReference type="OrthoDB" id="9947518at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045346">
        <property type="entry name" value="Ermin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008954">
        <property type="entry name" value="Moesin_tail_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20491">
        <property type="entry name" value="Ermin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017336251.1"/>
        </source>
    </evidence>
    <sequence length="297" mass="31644" checksum="06332E2AB282DB0B" modified="2017-12-20" version="1">MADPQAAPPTESPDHKPEPEPAAGEDKMPQSNAEEAATKQSSEESPNSDPEKPEEQKEQEEASAGADEKKAGEDAAEEKKEGEGGEDATEEKKEGEGGEDAAEEKKEGEGDGQNEDKVSEAGGEEQEGSSDRPGNKYYTVNYRKIKKGYAKQRISAFEALDFALLEEEGASEEQKDGATTEEGGAAANEANQANGEAGSQNGTSENADGQKAEDGQDGEKAEDGQDGEKAGEEAGDEQEEEDGIEDENGAEEECEGADGNKGKRAGISKYFTVSYRKIKKGLTKQRVDEFEFMGVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S2R7</accession>
    <name>A0A2D0S2R7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">adenylate cyclase</fullName>
            <ecNumber evidence="4 16">4.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">adcy2b</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the formation of the signaling molecule cAMP in response to G-protein signaling.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="17">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="17">Binds 2 magnesium ions per subunit. Is also active with manganese (in vitro).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16 18">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.1" evidence="4 16"/>
    <dbReference type="RefSeq" id="XP_017337027.2">
        <property type="nucleotide sequence ID" value="XM_017481538.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S2R7"/>
    <dbReference type="GeneID" id="108272792"/>
    <dbReference type="CTD" id="557902"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007193">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000010">
        <property type="entry name" value="Adenylate cyclase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032628">
        <property type="entry name" value="AC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030672">
        <property type="entry name" value="Adcy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009398">
        <property type="entry name" value="Adcy_conserved_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF6">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16214">
        <property type="entry name" value="AC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06327">
        <property type="entry name" value="Adcy_cons_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039050">
        <property type="entry name" value="Ade_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 16" id="KW-0115">cAMP biosynthesis</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15 16 18" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 16 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 16 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 16 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="96"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="187"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="650"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="677"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="728"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="785"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="810"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="853"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="21">
        <location>
            <begin position="340"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="21">
        <location>
            <begin position="938"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="20"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR039050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR039050-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017337027.2"/>
        </source>
    </evidence>
    <sequence length="1062" mass="120217" checksum="A6F29751E01AA83A" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S5K3</accession>
    <name>A0A2D0S5K3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mothers against decapentaplegic homolog</fullName>
            <shortName evidence="9">MAD homolog</shortName>
            <shortName evidence="9">Mothers against DPP homolog</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">SMAD family member</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">smad10a</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the dwarfin/SMAD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017338023.1">
        <property type="nucleotide sequence ID" value="XM_017482534.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S5K3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027339"/>
    <dbReference type="GeneID" id="108273360"/>
    <dbReference type="KEGG" id="ipu:108273360"/>
    <dbReference type="CTD" id="560317"/>
    <dbReference type="OrthoDB" id="5875866at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071144">
        <property type="term" value="C:heteromeric SMAD protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070411">
        <property type="term" value="F:I-SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
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        <location>
            <begin position="14"/>
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        <source>
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        <source>
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            <dbReference type="Proteomes" id="UP000221080"/>
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        <source>
            <dbReference type="RefSeq" id="XP_017338023.1"/>
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    <sequence length="561" mass="61315" checksum="3A180D961941B926" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SAZ4</accession>
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            <fullName evidence="4">Pdgfa associated protein 1a</fullName>
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        <name evidence="4" type="primary">pdap1a</name>
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        <name type="scientific">Ictalurus punctatus</name>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
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        <property type="entry name" value="Casein_kinase_sb_PP28"/>
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    </dbReference>
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            <begin position="1"/>
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        <location>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF10252"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017339902.1"/>
        </source>
    </evidence>
    <sequence length="159" mass="18386" checksum="CEF7C39EC1AC39E3" modified="2017-12-20" version="1">MPRGGKKGHKGRGKQFSNPEEIERQMRAQREMEENSGAERESGSESEEESSSEDEKEQKRSGVQGLIEIENPNRVSQKSKKAAEVDVNAPKELTRREREEIEKQKAKERYMKLHLEGKTDQAKADLARLAIIKKQREEAAKKREELRKEKEAQEAKTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SB17</accession>
    <name>A0A2D0SB17_ICTPU</name>
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        <submittedName>
            <fullName evidence="14">Galanin receptor 2a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC108274333</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
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                <person name="Scheffler B."/>
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                <person name="Flanagan M.E."/>
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                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Receptor for the hormone galanin. Receptor for the hormones spexin-1 and spexin-2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017339932.1">
        <property type="nucleotide sequence ID" value="XM_017484443.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SB17"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006148"/>
    <dbReference type="GeneID" id="108274333"/>
    <dbReference type="KEGG" id="ipu:108274333"/>
    <dbReference type="OMA" id="LTYTRTI"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004966">
        <property type="term" value="F:galanin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15097">
        <property type="entry name" value="7tmA_Gal2_Gal3_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000092">
        <property type="entry name" value="Galanin receptor type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000405">
        <property type="entry name" value="Galanin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF35">
        <property type="entry name" value="GALANIN RECEPTOR TYPE 2-LIKE ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00663">
        <property type="entry name" value="GALANINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="34"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017339932.1"/>
        </source>
    </evidence>
    <sequence length="362" mass="40814" checksum="E50148DA75E4B5E8" modified="2017-12-20" version="1">MNASQQIHFSSNRRVETAIIASVFALIFVLGTVGNCLVLAVLLRNGQMNGKTTNMFILNLGLADLCFILFCVPLQATIYSMDEWVFGAFVCKVVHFIIYLTMYASIFTLTAVSLDRYLAICYPLHARELRTPHNTLASIGVVWTLSLVFSGPYFSYYQQMDLNGTTVCIPAWDIQSRRAMDICTFVFGYLVPVLVLGLTYARTIRYLWTSVDPMQEACESRLAKRRVTKMIVIVAVLFCLCWLPHHLIIMCMWFGHFPLNHATYVLRILSHLVAYTNSCLNPIVYALVSKHFRKGFRKVFGCISQKRLANQVNMVPQAQTVSLVEVGSSDGSNQSDASAKVRFLSKSHEKTTLSAFMTFNVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SC60</accession>
    <name>A0A2D0SC60_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Carbohydrate sulfotransferase</fullName>
            <ecNumber evidence="11">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC108274608</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Golgi apparatus membrane</location>
            <topology evidence="1 11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sulfotransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_017340314.1">
        <property type="nucleotide sequence ID" value="XM_017484825.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SC60"/>
    <dbReference type="GeneID" id="108274608"/>
    <dbReference type="KEGG" id="ipu:108274608"/>
    <dbReference type="OrthoDB" id="2019940at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030166">
        <property type="term" value="P:proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018011">
        <property type="entry name" value="Carb_sulfotrans_8-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137:SF4">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9 11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017340314.1"/>
        </source>
    </evidence>
    <sequence length="378" mass="44618" checksum="4258A706EA6AED44" modified="2017-12-20" version="1">MLRLSHLPDEMGKFKYTCYLLFILSFISVLFYINRQAEDGHERYIYAKPQDSSKPEMKPRLDTEENGRCEGKIHTNPMPPKLTLRQYARKKLVYDLCSTKTILDTRSKWQTFDQIPKLELYNLLVDDRHGIIYCYVPKVACTAWKRIMIVLSESLKVHGVPYRVPADVPIEDVHGSSLQHLNEYPKVVMKQKLETYKKFIFVRDPFVRLISAYRDKLESPNQIYYERIGMDILRTFGNVSNPPASVEKAHVDGIVPSFYNFIQYILSLSAFNDTVFDEHWRQTINLCHPCLINYDFIGKMETIEEDAAQLLRILHVDNIVDFKPWVKSKTGPREIMTWFANIPLEWRRKLYKVYEEDFRLFGYENHEHLDDLNLGNIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SCI9</accession>
    <name>A0A2D0SCI9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein FAM180A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC108274499</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017340186.1">
        <property type="nucleotide sequence ID" value="XM_017484697.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SCI9"/>
    <dbReference type="GeneID" id="108274499"/>
    <dbReference type="KEGG" id="ipu:108274499"/>
    <dbReference type="OMA" id="WPDFEKT"/>
    <dbReference type="OrthoDB" id="8913792at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029170">
        <property type="entry name" value="FAM180"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34034:SF2">
        <property type="entry name" value="PROTEIN FAM180A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34034">
        <property type="entry name" value="PROTEIN FAM180A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15173">
        <property type="entry name" value="FAM180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012180896" evidence="2">
        <location>
            <begin position="23"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="68"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017340186.1"/>
        </source>
    </evidence>
    <sequence length="169" mass="19606" checksum="D9756F6CDE781DF7" modified="2017-12-20" version="1">METTLALVILSVVLWHLHISAALHWRRVLYPSAFRTKRGTPALLNPSIQRSVEDANLLYEVVWSGLYVEAEKNVLRVADEELASLRRLQPLEVVCEDVLPRTLSDIRRLCHNLEQRRARMSKEDFERTVLTMVYTAERLAQSSTGHQRELWADALLQLYKRIKKDLGTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SCL2</accession>
    <name>A0A2D0SCL2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Transmembrane protein 17B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">tmem17</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Transmembrane component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Required for ciliogenesis and sonic hedgehog/SHH signaling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the tectonic-like complex (also named B9 complex).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the TMEM17 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017340206.1">
        <property type="nucleotide sequence ID" value="XM_017484717.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SCL2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027638"/>
    <dbReference type="GeneID" id="108274511"/>
    <dbReference type="KEGG" id="ipu:108274511"/>
    <dbReference type="CTD" id="200728"/>
    <dbReference type="OMA" id="LWWVSCI"/>
    <dbReference type="OrthoDB" id="311720at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019184">
        <property type="entry name" value="Uncharacterised_TM-17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13531">
        <property type="entry name" value="GEO07735P1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13531:SF14">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09799">
        <property type="entry name" value="Transmemb_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017340206.1"/>
        </source>
    </evidence>
    <sequence length="191" mass="22064" checksum="897814F3AB59EE94" modified="2017-12-20" version="1">MGLPEPIRRRLGDFSRTVFVNQSHSQLSPEDHITFLDHNTDVVSSLPLQMALFFNMCFFPLWWISEVVMLHLKYPALPDYYKVILITVVILMTLIEAIRLYLGYAGNLQEKVPELAGFWLLSLLLQFPLILFQLFNEAILIQPLERGVHIVLALFILTEALSGFVALRAMVRHTESRFHLSQFNGIQDHRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SDF9</accession>
    <name>A0A2D0SDF9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">heparosan-N-sulfate-glucuronate 5-epimerase</fullName>
            <ecNumber evidence="6">5.1.3.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">glcea</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[heparosan-N-sulfate](n) = [heparan-N-sulfate](n)</text>
            <dbReference type="Rhea" id="RHEA:20197"/>
            <dbReference type="Rhea" id="RHEA-COMP:9556"/>
            <dbReference type="Rhea" id="RHEA-COMP:9557"/>
            <dbReference type="ChEBI" id="CHEBI:58041"/>
            <dbReference type="ChEBI" id="CHEBI:58287"/>
            <dbReference type="EC" id="5.1.3.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Glycan metabolism; heparan sulfate biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycan metabolism; heparin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Endomembrane system</location>
            <topology evidence="12">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the D-glucuronyl C5-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.17" evidence="6"/>
    <dbReference type="RefSeq" id="XP_017340290.1">
        <property type="nucleotide sequence ID" value="XM_017484801.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SDF9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000014940"/>
    <dbReference type="GeneID" id="108274589"/>
    <dbReference type="KEGG" id="ipu:108274589"/>
    <dbReference type="CTD" id="405776"/>
    <dbReference type="OMA" id="VKATRIM"/>
    <dbReference type="OrthoDB" id="5914444at2759"/>
    <dbReference type="UniPathway" id="UPA00862"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047464">
        <property type="term" value="F:heparosan-N-sulfate-glucuronate 5-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009950">
        <property type="term" value="P:dorsal/ventral axis specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015012">
        <property type="term" value="P:heparan sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030210">
        <property type="term" value="P:heparin proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010598">
        <property type="entry name" value="C5-epim_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039721">
        <property type="entry name" value="C5-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059154">
        <property type="entry name" value="Glce_b_sandwich"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13174">
        <property type="entry name" value="D-GLUCURONYL C5-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13174:SF3">
        <property type="entry name" value="D-GLUCURONYL C5-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06662">
        <property type="entry name" value="C5-epim_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21174">
        <property type="entry name" value="Glce_b_sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="D-glucuronyl C5-epimerase beta-sandwich" evidence="15">
        <location>
            <begin position="236"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="D-glucuronyl C5-epimerase C-terminal" evidence="14">
        <location>
            <begin position="383"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF06662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF21174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017340290.1"/>
        </source>
    </evidence>
    <sequence length="584" mass="65811" checksum="5A4B44F06C9E656A" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SN15</accession>
    <name>A0A2D0SN15_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Battenin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cln3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Lysosome membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the battenin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017344097.1">
        <property type="nucleotide sequence ID" value="XM_017488608.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SN15"/>
    <dbReference type="GeneID" id="108276732"/>
    <dbReference type="KEGG" id="ipu:108276732"/>
    <dbReference type="CTD" id="1201"/>
    <dbReference type="OrthoDB" id="5965864at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007040">
        <property type="term" value="P:lysosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003492">
        <property type="entry name" value="Battenin_disease_Cln3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018460">
        <property type="entry name" value="Battenin_disease_Cln3_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10981">
        <property type="entry name" value="BATTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10981:SF0">
        <property type="entry name" value="BATTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02487">
        <property type="entry name" value="CLN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015974">
        <property type="entry name" value="CLN3_BTN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01315">
        <property type="entry name" value="BATTENIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0458">Lysosome</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="383"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="419"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017344097.1"/>
        </source>
    </evidence>
    <sequence length="449" mass="49497" checksum="A83DCBF2925DCE6A" modified="2017-12-20" version="1">MDHRDSVNADPDMLTGSEGRCRRCRNQSGFWLLGLCNNFAYVVMLSAAHDILKQQESQNATMPTPGPVPSDGGNSSNSSRYDCNPVSTAAVLLADILPTLLIKLTAPFFIHKVPYGFRILGCVATATTSFLVVSFSSTVWMSILGVIFASVSSGLGELSFLSLTVYFSSDVLSGWGSGTGGAGVAGAVLYSAFTQAGLSPRGTLLIMLLIPAVLMISYFRLLDFPPSFPQWRSGSGEDVQERRPLIVDDNDEDSVRDVNGKDNCAGNAFSFLTEDQSTEPLTFLDKLHILKRLLKFTVPLCVVYFAEYFINQGLLELLYFPASSLSHAEQYRWYQTLYQIGVFISRTSLYCVKTRRIWVLSMLQCVNAVVLLISVYYRFWPNVWVVFVIVLYEGLLGGAVYVNTFYLIRVETVEREREFAMAAASVGDSIGIALSAAPAIPIHDYFCSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SQK0</accession>
    <name>A0A2D0SQK0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Vasodilator-stimulated phosphoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">vaspa</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">SDA103</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="27">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">Homotetramer. Interacts with PFN1, PFN2, LPP, ACTN1 and ACTG1. Interacts, via the EVH1 domain, with the Pro-rich regions of ZYX. This interaction is important for targeting to focal adhesions and the formation of actin-rich structures at the apical surface of cells. Interacts, via the EVH1 domain, with the Pro-rich domain of Listeria monocytogenes actA. Interacts with APBB1IP. Interacts, via the Pro-rich domain, with the C-terminal SH3 domain of DNMBP. Interacts weakly with MEFV.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell junction</location>
            <location evidence="4">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Cell projection</location>
            <location evidence="19">Filopodium membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Lamellipodium membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Ena/VASP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017344565.2">
        <property type="nucleotide sequence ID" value="XM_017489076.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SQK0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005093"/>
    <dbReference type="GeneID" id="108276935"/>
    <dbReference type="KEGG" id="ipu:108276935"/>
    <dbReference type="CTD" id="555805"/>
    <dbReference type="OrthoDB" id="31170at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031527">
        <property type="term" value="C:filopodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031258">
        <property type="term" value="C:lamellipodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005522">
        <property type="term" value="F:profilin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001843">
        <property type="term" value="P:neural tube closure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030838">
        <property type="term" value="P:positive regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01207">
        <property type="entry name" value="EVH1_Ena_VASP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000005">
        <property type="entry name" value="vasodilator-stimulated phosphoprotein isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000047">
        <property type="entry name" value="vasodilator-stimulated phosphoprotein isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038023">
        <property type="entry name" value="VASP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014885">
        <property type="entry name" value="VASP_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000697">
        <property type="entry name" value="WH1/EVH1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202">
        <property type="entry name" value="SPROUTY-RELATED, EVH1 DOMAIN-CONTAINING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202:SF12">
        <property type="entry name" value="VASODILATOR-STIMULATED PHOSPHOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08776">
        <property type="entry name" value="VASP_tetra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00568">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00461">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118370">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein, VASP, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50229">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="16" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="11" id="KW-0965">Cell junction</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="14 23" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0729">SH3-binding</keyword>
    <keyword evidence="6" id="KW-0796">Tight junction</keyword>
    <feature type="domain" description="WH1" evidence="25">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="113"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="23">
        <location>
            <begin position="374"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="130"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="24">
        <location>
            <begin position="162"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="24">
        <location>
            <begin position="183"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="24">
        <location>
            <begin position="219"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="24">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="318"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00046311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00059051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00061997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00074299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_017344565.2"/>
        </source>
    </evidence>
    <sequence length="409" mass="42339" checksum="D7E939029BFF1A27" modified="2023-06-28" version="2">MSEVSLCQARATVMLYDDAGKRWVPAGTGGQAVSRVHIYQNPSSNTFRVVGRKLQADQQVVINCPLMRGIKYNQATPNFHQWRDVRQVWGLNFGSKDEAMQFANTMLHALDLVNGATPSRPPPNGPSVEEQQRQEQQERERQERERQEQERLERQRQAAAPPAAPPAAPPAPPPAPAAAAPPAGVPPPPGPPPPPGPPPAPPPPPADGAPPAPPPPPSGGGGGGGGFGGAGGLASALAGAKLRKVSKDESGSGAPAPKESSQSSGGGGGGGGGGGGGGGGGGGLMGEMSALLARRRKVTDTPVAKKEDNNDNSSPSENKTEVCKPWEKSGTLPRSNSGPKGLDKSPAPASAPINRQRPGSNVSDTAARESFDMEKFKQEILEEVRKELQKVKDEIIGAFIEELQKGGAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SQN9</accession>
    <name>A0A2D0SQN9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Avidin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">avd</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the avidin/streptavidin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017345018.1">
        <property type="nucleotide sequence ID" value="XM_017489529.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SQN9"/>
    <dbReference type="GeneID" id="108277130"/>
    <dbReference type="KEGG" id="ipu:108277130"/>
    <dbReference type="CTD" id="567678"/>
    <dbReference type="OrthoDB" id="2821340at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009374">
        <property type="term" value="F:biotin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.30">
        <property type="entry name" value="Avidin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005469">
        <property type="entry name" value="Avidin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036896">
        <property type="entry name" value="Avidin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005468">
        <property type="entry name" value="Avidin/str"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051764">
        <property type="entry name" value="Avidin/Streptavidin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34399:SF3">
        <property type="entry name" value="AVID PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34399">
        <property type="entry name" value="AVIDIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01382">
        <property type="entry name" value="Avidin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00709">
        <property type="entry name" value="AVIDIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50876">
        <property type="entry name" value="Avidin/streptavidin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51326">
        <property type="entry name" value="AVIDIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0092">Biotin</keyword>
    <keyword evidence="5 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5011977075" evidence="9">
        <location>
            <begin position="24"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="35"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR605468-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017345018.1"/>
        </source>
    </evidence>
    <sequence length="157" mass="16844" checksum="F758A1D068B857C9" modified="2017-12-20" version="1">MESFVVCLCVLALGMCQIIIGQALISDSERKVLDCNVTGEWHSELGSQLQLSTVGPEVRGVYRTVLESTRGAAGPNREAKVVGVISDGPQPTIAFSALWAKGSCTTWVGQCFILPGGVQVLKMLWLLRSAAESSVDNWDSTRLGADQFIFARGVESP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SR12</accession>
    <name>A0A2D0SR12_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Histone H2B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC108277172</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="12">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017345147.1">
        <property type="nucleotide sequence ID" value="XM_017489658.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SR12"/>
    <dbReference type="GeneID" id="108277172"/>
    <dbReference type="KEGG" id="ipu:108277172"/>
    <dbReference type="OrthoDB" id="10036053at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000003">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 12" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11 12" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="14">
        <location>
            <begin position="20"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="13"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017345147.1"/>
        </source>
    </evidence>
    <sequence length="126" mass="13978" checksum="67F2148B27C5071C" modified="2017-12-20" version="1">MPEPSKTAPAPKKGSKKAVTKTQKKGDKKRRKTRKESYAIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SR52</accession>
    <name>A0A2D0SR52_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Heat shock cognate 71 kDa protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC108277187</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017345173.1">
        <property type="nucleotide sequence ID" value="XM_017489684.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SR52"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009332"/>
    <dbReference type="GeneID" id="108277187"/>
    <dbReference type="KEGG" id="ipu:108277187"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10233">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000001">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000028">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000135">
        <property type="entry name" value="Heat shock cognate 71 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000134">
        <property type="entry name" value="Heat shock cognate 71 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000003">
        <property type="entry name" value="heat shock cognate 71 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000026">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="614"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="616"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017345173.1"/>
        </source>
    </evidence>
    <sequence length="646" mass="70830" checksum="77E95DFD39A1F2FB" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SWU3</accession>
    <name>A0A2D0SWU3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Galactose-3-O-sulfotransferase 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">gal3st2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the galactose-3-O-sulfotransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347154.1">
        <property type="nucleotide sequence ID" value="XM_017491665.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SWU3"/>
    <dbReference type="GeneID" id="108278346"/>
    <dbReference type="KEGG" id="ipu:108278346"/>
    <dbReference type="CTD" id="64090"/>
    <dbReference type="OrthoDB" id="514299at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001733">
        <property type="term" value="F:galactosylceramide sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009729">
        <property type="entry name" value="Gal-3-0_sulfotransfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14647">
        <property type="entry name" value="GALACTOSE-3-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14647:SF62">
        <property type="entry name" value="GALACTOSE-3-O-SULFOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06990">
        <property type="entry name" value="Gal-3-0_sulfotr"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="37"/>
            <end position="58"/>
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        <location>
            <begin position="107"/>
            <end position="151"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="164"/>
            <end position="198"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="591"/>
            <end position="631"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
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            <begin position="107"/>
            <end position="122"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
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            <begin position="166"/>
            <end position="176"/>
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            <end position="603"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    <evidence type="ECO:0000313" key="13">
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SYL7</accession>
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        <submittedName>
            <fullName evidence="22">Gamma-aminobutyric acid receptor subunit beta-4</fullName>
        </submittedName>
    </protein>
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        <name evidence="22" type="primary">gabrb4</name>
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        <name type="scientific">Ictalurus punctatus</name>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="21">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
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        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
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        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="entry name" value="GABAARECEPTR"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="13" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0869">Chloride channel</keyword>
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    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10 22" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 18" id="KW-0732">Signal</keyword>
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    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022271171" evidence="18">
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            <begin position="31"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="243"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="308"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="493"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="19">
        <location>
            <begin position="39"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="20">
        <location>
            <begin position="250"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017347430.1"/>
        </source>
    </evidence>
    <sequence length="512" mass="58469" checksum="C47774B3253FA9B0" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SZA6</accession>
    <name>A0A2D0SZA6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein transport protein sec16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21 22" type="primary">sec16a</name>
    </gene>
    <organism evidence="20 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
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        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21 22" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21 22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Plays a role in the organization of the endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER). Required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">SEC16A and SEC16B are each present in multiple copies in a heteromeric complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Microsome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 16">Belongs to the SEC16 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347860.1">
        <property type="nucleotide sequence ID" value="XM_017492371.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017347861.1">
        <property type="nucleotide sequence ID" value="XM_017492372.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000007040"/>
    <dbReference type="GeneID" id="108278785"/>
    <dbReference type="KEGG" id="ipu:108278785"/>
    <dbReference type="CTD" id="9919"/>
    <dbReference type="OrthoDB" id="8918678at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007029">
        <property type="term" value="P:endoplasmic reticulum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051668">
        <property type="term" value="P:localization within membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070973">
        <property type="term" value="P:protein localization to endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09233">
        <property type="entry name" value="ACE1-Sec16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1030:FF:000002">
        <property type="entry name" value="Protein transport protein sec16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024340">
        <property type="entry name" value="Sec16_CCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024298">
        <property type="entry name" value="Sec16_Sec23-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13402:SF13">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC16A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13402">
        <property type="entry name" value="RGPR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12932">
        <property type="entry name" value="Sec16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12931">
        <property type="entry name" value="TPR_Sec16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12 16" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="14 16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0492">Microsome</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13 16" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Sec16 central conserved" evidence="19">
        <location>
            <begin position="1208"/>
            <end position="1305"/>
        </location>
    </feature>
    <feature type="domain" description="Sec16 Sec23-binding" evidence="18">
        <location>
            <begin position="1376"/>
            <end position="1610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="370"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="595"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="678"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="744"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="828"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1113"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1651"/>
            <end position="1672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1729"/>
            <end position="2001"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="2053"/>
            <end position="2166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="149"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="173"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="197"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="220"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="395"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="634"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="692"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="748"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="839"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="851"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="879"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="982"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="993"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1113"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1127"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1653"/>
            <end position="1664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="1729"/>
            <end position="1738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1786"/>
            <end position="1810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1822"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1907"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1931"/>
            <end position="1951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="17">
        <location>
            <begin position="1982"/>
            <end position="1992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="2066"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="2099"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="2140"/>
            <end position="2150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU364101"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF12931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF12932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017347860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017347861.1"/>
        </source>
    </evidence>
    <sequence length="2166" mass="235111" checksum="20C0503AC08C8AD1" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T485</accession>
    <name>A0A2D0T485_ICTPU</name>
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        <submittedName>
            <fullName evidence="3 4 5">U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 1-like isoform X1</fullName>
        </submittedName>
    </protein>
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        <name evidence="3 4 5 6" type="primary">LOC108279616</name>
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    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
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    </reference>
    <reference evidence="3 4 5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3 4 5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017349420.1">
        <property type="nucleotide sequence ID" value="XM_017493931.3"/>
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        <property type="nucleotide sequence ID" value="XM_053688380.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053544356.1">
        <property type="nucleotide sequence ID" value="XM_053688381.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053544357.1">
        <property type="nucleotide sequence ID" value="XM_053688382.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="108279616"/>
    <dbReference type="KEGG" id="ipu:108279616"/>
    <dbReference type="OrthoDB" id="8947254at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017349420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053544355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053544356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_053544357.1"/>
        </source>
    </evidence>
    <sequence length="140" mass="17127" checksum="DAB76B06AD4A6E4E" modified="2017-12-20" version="1">MNHMKSFAAIPMSKCMRDMCKEEDYAFLNNQKSREIDKTPKESTFREREWQQKQEERLRRRLDREQQEQEKQCELEKQRREKEEQWKSHVAGLASERESLRGRLHRLREFRDFQKRVLMQDLGLEPGTASENLTQLLMRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T6A5</accession>
    <name>A0A2D0T6A5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Junctional adhesion molecule-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC108280129</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017350427.1">
        <property type="nucleotide sequence ID" value="XM_017494938.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T6A5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012991"/>
    <dbReference type="GeneID" id="108280129"/>
    <dbReference type="KEGG" id="ipu:108280129"/>
    <dbReference type="OrthoDB" id="6157407at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042130">
        <property type="term" value="P:negative regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042102">
        <property type="term" value="P:positive regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031295">
        <property type="term" value="P:T cell costimulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051713">
        <property type="entry name" value="T-cell_Activation_Regulation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466:SF14">
        <property type="entry name" value="BUTYROPHILIN SUBFAMILY 2 MEMBER A2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466">
        <property type="entry name" value="T-LYMPHOCYTE ACTIVATION ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012497342" evidence="13">
        <location>
            <begin position="18"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="35"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="138"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="226"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="226"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="241"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017350427.1"/>
        </source>
    </evidence>
    <sequence length="352" mass="39186" checksum="3B179644DCB6A435" modified="2017-12-20" version="1">MMKCLYLLSVVFHVAAGCSLSGDNLRQITEIYRHKGGSVLFPCSCSDLNTKPQKFTWESFRTGRLTEVLNDEHYRDRLQLFNYSSPANLSLLISDLREEDHGVYRCSTEKDHRDIRLNVKGCELVKKTGIEHMTVFTGESVVLPCVCTDLQDKPMSVKWEFSIPATGISHFQEIYPEQTGRHRNRVKLVSNNAPGNLSLLISDLTEEDQGDYRCSVQADSRDVRLSVKVGKRETSSEPSQSKTTNSPPASSSTALEQDKQQTHSSLPLVFGILAVLLLVIPGVVAFICWRRKGGRCGENVITEGRPDCEVKQKDQTEPDVTYSTVTHVNTAGAARVQINAADNTEYASVITN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T6D2</accession>
    <name>A0A2D0T6D2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Teleost multiple tissue opsin a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">tmtopsa</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017350454.1">
        <property type="nucleotide sequence ID" value="XM_017494965.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T6D2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000016394"/>
    <dbReference type="GeneID" id="108280164"/>
    <dbReference type="KEGG" id="ipu:108280164"/>
    <dbReference type="CTD" id="359832"/>
    <dbReference type="OrthoDB" id="2101615at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15086">
        <property type="entry name" value="7tmA_tmt_opsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000197">
        <property type="entry name" value="Teleost multiple tissue opsin 2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050125">
        <property type="entry name" value="GPCR_opsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008365">
        <property type="entry name" value="Prostanoid_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027430">
        <property type="entry name" value="Retinal_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24240">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01788">
        <property type="entry name" value="PROSTANOIDR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00238">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0157">Chromophore</keyword>
    <keyword evidence="9 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="11 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0681">Retinal protein</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="13 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 14 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="297"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="383"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="152"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="456"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="457"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017350454.1"/>
        </source>
    </evidence>
    <sequence length="489" mass="53216" checksum="398D674BC776884F" modified="2017-12-20" version="1">MASEGLQNKISLSLSLCVCVCVCVYVWDPSNHHTVEQSHSKLLTFSSNEALKQNRMPISVPFLKSLGSFPRTPSTNTTVSFPHVNSRNCVCVCQCARPAMVWSNGSAGGCCGSPEYVSDTGAARDSELSLSPTGHSVVAACLGLISTVGFVNNLLALVLFGRHKALRSPINLLLINISLSDMLVCALATPFSLAASTRGRWLAGRTACVWYGFANSLFGIVSLISLAVLSYERYCTMMCPAKPDVTNYTKTAVGVVLSWVYSLVWTLPPFFGWSRYGPEGPGTTCSVDWVTKTANNISYIICLFVFCLILPFLVIVYSYGKLLHAIKQVSRLKTSTGRKREHRVLLMVLTMVVCYLLCWLPYGIMALLATFGAPGLVTAEASIVPSVLAKTSTVINPVIYVFMNKQFNRCFRSFLKCDVVQQSSTSKRWSKADKPVRRTDNNLTFVAASVDHGLAAPSTSRSNEQPPSTDSAKPPALSLVAHYNGSFCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T828</accession>
    <name>A0A2D0T828_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Sorcin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">sri</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Peripheral membrane protein</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017350790.1">
        <property type="nucleotide sequence ID" value="XM_017495301.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T828"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005101"/>
    <dbReference type="GeneID" id="108280385"/>
    <dbReference type="KEGG" id="ipu:108280385"/>
    <dbReference type="CTD" id="6717"/>
    <dbReference type="OMA" id="LNQFIYC"/>
    <dbReference type="OrthoDB" id="186625at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16181">
        <property type="entry name" value="EFh_PEF_Group_II_sorcin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000087">
        <property type="entry name" value="Sorcin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46735">
        <property type="entry name" value="CALPAIN, SMALL SUBUNIT 1 A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46735:SF7">
        <property type="entry name" value="SORCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="90"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017350790.1"/>
        </source>
    </evidence>
    <sequence length="188" mass="20886" checksum="22AD6B10C798A4B9" modified="2017-12-20" version="1">MSFPGYGAPAGGYPGAFPGQQQDPLFGYFTAIAGQDGQISAEELQVCLTQANFSGGYKPFNLETCRLMIAMLDRDMSGNMGFAEFKELWAVLNGWKQHFMSIDKDMSGTVDPQEMNQAISSMGYRLSPQAMNCIVRRYSTQGKITFDDYVACCVKLRSLTDIFRRRDPAQGGTASFQYDDFIQCTMST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T9H5</accession>
    <name>A0A2D0T9H5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Nucleotidyltransferase MB21D2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">mb21d2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mab-21 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017351521.2">
        <property type="nucleotide sequence ID" value="XM_017496032.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T9H5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018453"/>
    <dbReference type="GeneID" id="108280705"/>
    <dbReference type="KEGG" id="ipu:108280705"/>
    <dbReference type="CTD" id="151963"/>
    <dbReference type="OrthoDB" id="269173at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046903">
        <property type="entry name" value="Mab-21-like_nuc_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046906">
        <property type="entry name" value="Mab-21_HhH/H2TH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024810">
        <property type="entry name" value="MAB21L/cGLR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656">
        <property type="entry name" value="CELL FATE DETERMINING PROTEIN MAB21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656:SF47">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE MB21D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03281">
        <property type="entry name" value="Mab-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20266">
        <property type="entry name" value="Mab-21_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01265">
        <property type="entry name" value="Mab-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mab-21-like nucleotidyltransferase" evidence="3">
        <location>
            <begin position="222"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Mab-21-like HhH/H2TH-like" evidence="4">
        <location>
            <begin position="379"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="470"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="470"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017351521.2"/>
        </source>
    </evidence>
    <sequence length="545" mass="61491" checksum="45BE9E0232BC75AD" modified="2023-02-22" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0TAP7</accession>
    <name>A0A2D0TAP7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Iroquois-class homeodomain protein IRX-1a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">irx1a</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/IRO homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017351948.1">
        <property type="nucleotide sequence ID" value="XM_017496459.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0TAP7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000094"/>
    <dbReference type="GeneID" id="108280964"/>
    <dbReference type="KEGG" id="ipu:108280964"/>
    <dbReference type="CTD" id="403019"/>
    <dbReference type="OrthoDB" id="5399138at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000003">
        <property type="entry name" value="Iroquois-class homeobox protein IRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003893">
        <property type="entry name" value="Iroquois_homeo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211">
        <property type="entry name" value="IROQUOIS-CLASS HOMEODOMAIN PROTEIN IRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211:SF13">
        <property type="entry name" value="IROQUOIS-CLASS HOMEODOMAIN PROTEIN IRX-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00548">
        <property type="entry name" value="IRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="131"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="133"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="197"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="358"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="211"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="223"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="238"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="359"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="370"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="383"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017351948.1"/>
        </source>
    </evidence>
    <sequence length="429" mass="47114" checksum="EDB83ACD7F9D5745" modified="2017-12-20" version="1">MSFPQLGYPQYLYGVGAAADRQLLAPSSVSPASPPSSRGANCDVAGSACAVTSLLAAGAGGAGGVYAHPYGYGAFLPYTSAAELALFSQMGSQYDLKDSPGVSFAAHASPAAFYPYAGFQYGDASRPKNATRESTSTLKAWLNEHRKNPYPTKGEKIMLAIITKMTLTQVSTWFANARRRLKKENKVTWGARAKEDGEDGAIFGSDNEDKREDEEEIDLESIDIDKIDENDGDRSNAEEEEDDDEDDEDEDKRARKLLKERDDAHAHANAHGNANKNMTIMTSPSLQLPVSSKPKIWSLAETATSPDVSKNAGVQHPAFLANHRLYTCPIGKIHSWNNSAFLSHNSLLNVRSLVGVNQNHHHHHHHHHQTQQQQPQQQQQGVMEKDKSPEQLSPKHTERGVESPTHTITKSSFRPLHDGPRNPHRVVTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EEH4</accession>
    <name>A0A979EEH4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Plectin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Plectin-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">pleca</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">SDA103</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="27">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Involved in the organization of desmosome cell-cell junctions. Of particular importance in cell adhesion in the skin and during cardiac development. May also play a role in the regulation of Wnt, TGF-beta and Hippo signaling pathways.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Desmosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Cell junction</location>
            <location evidence="17">Hemidesmosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Podosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the plakin or cytolinker family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047006612.2">
        <property type="nucleotide sequence ID" value="XM_047150656.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108256925"/>
    <dbReference type="CTD" id="567639"/>
    <dbReference type="OrthoDB" id="18740at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030057">
        <property type="term" value="C:desmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030056">
        <property type="term" value="C:hemidesmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002102">
        <property type="term" value="C:podosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030506">
        <property type="term" value="F:ankyrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008307">
        <property type="term" value="F:structural constituent of muscle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002934">
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        <property type="entry name" value="PCN-like_spectrin-like_rpt"/>
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        <property type="entry name" value="Plakin"/>
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        <property type="entry name" value="Spectrin/alpha-actinin"/>
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        <property type="entry name" value="ENVOPLAKIN"/>
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        <property type="entry name" value="PLECTIN ISOFORM X1"/>
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        <property type="entry name" value="SH3_10"/>
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        <property type="entry name" value="SR_plectin_7"/>
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        <property type="match status" value="4"/>
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        <property type="match status" value="4"/>
    </dbReference>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
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    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="23" id="KW-0472">Membrane</keyword>
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    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 20" id="KW-0728">SH3 domain</keyword>
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        <location>
            <begin position="17"/>
            <end position="38"/>
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    <feature type="transmembrane region" description="Helical" evidence="23">
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            <begin position="420"/>
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        </location>
    </feature>
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            <begin position="541"/>
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            <begin position="1193"/>
            <end position="1250"/>
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            <begin position="55"/>
            <end position="75"/>
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    <feature type="region of interest" description="Disordered" evidence="22">
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            <end position="331"/>
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    <feature type="region of interest" description="Disordered" evidence="22">
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    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="2045"/>
            <end position="2084"/>
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        <location>
            <begin position="2174"/>
            <end position="2193"/>
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            <end position="2224"/>
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    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="2415"/>
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        <location>
            <begin position="2479"/>
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        <location>
            <begin position="4871"/>
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            <begin position="1638"/>
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        <location>
            <begin position="56"/>
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    </feature>
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        <location>
            <begin position="98"/>
            <end position="319"/>
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        <location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004188"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004568"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009109"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
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    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
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    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
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            <dbReference type="ARBA" id="ARBA00022990"/>
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            <dbReference type="ARBA" id="ARBA00023054"/>
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            <dbReference type="ARBA" id="ARBA00023203"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00056058"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00060371"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00071000"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00081353"/>
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    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
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    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_047006612.2"/>
        </source>
    </evidence>
    <sequence length="4972" mass="568652" checksum="6F26278F36A973B0" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EXS6</accession>
    <name>A0A979EXS6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Probable G-protein coupled receptor 25</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">gpr25</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047012570.1">
        <property type="nucleotide sequence ID" value="XM_047156614.2"/>
    </dbReference>
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    <dbReference type="GeneID" id="108267152"/>
    <dbReference type="KEGG" id="ipu:108267152"/>
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    <dbReference type="OrthoDB" id="8935849at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
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            <dbReference type="RefSeq" id="XP_047012570.1"/>
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    <sequence length="381" mass="42989" checksum="A777068444BF4067" modified="2023-02-22" version="1">MASSTEVAFFHTTMEAVTDDLYHYYDYELNDTVSPTSVTAVKEQPLAMSHIYLPMLYFIMFFAGFTGNLFVIIVIGNRHKKNARLVDTFVLNLALADLVFVFTLPLWAISAGKRDQWPFGELLCKTSSYIIAVNRFSNIFFLTCMSVDRYLAIVRLMDSHFLRSSKCIHLTCGVVWGSSLILGTPSLVYRKLNVEYGFCIDELESSFFQGMILLTVFLSFVLPVMIILLSYGSIMVKLRHHCVTAGNSRAEARCRHSLKIVFTIIIVFLVSWLPYNVLRTIQVICKISNGKLDNNTYRYLARGLIISSCLAFLNSCLNPVIYLFLDKHFRRSAASLCKSCTGKRDKQQSYMSSNSTASNGISESLGSTAARGHIFPLTQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F4Q7</accession>
    <name>A0A979F4Q7_ICTPU</name>
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        <submittedName>
            <fullName evidence="10">NACHT, LRR and PYD domains-containing protein 12 isoform X1</fullName>
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        <name evidence="10" type="primary">LOC108272977</name>
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        <name type="scientific">Ictalurus punctatus</name>
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        <dbReference type="NCBI Taxonomy" id="7998"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
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            <taxon>Ictaluridae</taxon>
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    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="10">Blood</tissue>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
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        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
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        <property type="entry name" value="NOD1/2_WH"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="WHD_NOD2"/>
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    </dbReference>
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        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="8">
        <location>
            <begin position="263"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="37"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="67"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="106"/>
            <end position="133"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_047014870.1"/>
        </source>
    </evidence>
    <sequence length="1119" mass="124086" checksum="C7E6BBED271D3C9C" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F4U3</accession>
    <name>A0A979F4U3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein LOC124628740</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC124628740</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
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            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047014910.2">
        <property type="nucleotide sequence ID" value="XM_047158954.2"/>
    </dbReference>
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    <dbReference type="GeneID" id="124628740"/>
    <dbReference type="KEGG" id="ipu:124628740"/>
    <dbReference type="OrthoDB" id="10468497at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_047014910.2"/>
        </source>
    </evidence>
    <sequence length="161" mass="17608" checksum="A1A8B5F6331E11E2" modified="2023-06-28" version="2">MHPEHISSSTSNKSSSNTTSSHSVSSPETHTGVTNPSSTNNKDSRTEHVLTNSSPSSNQGEGVPGWAIALLVLAAIAILLLIIIIIILLLRWCCVDNGDDVRSLPPQEHTPYMRTTFREPLSVPAYSPHTPQKNLYPFDDLPQSPKPKPNRTGTYEVNPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979FDY1</accession>
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        <recommendedName>
            <fullName evidence="14">C-Jun-amino-terminal kinase-interacting protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">JNK MAP kinase scaffold protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">mapk8ip3</name>
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    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
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    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell projection</location>
            <location evidence="6">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the JIP scaffold family.</text>
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        <property type="nucleotide sequence ID" value="XM_047162490.2"/>
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    <dbReference type="KEGG" id="ipu:108255773"/>
    <dbReference type="CTD" id="23162"/>
    <dbReference type="OrthoDB" id="10256043at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005078">
        <property type="term" value="F:MAP-kinase scaffold activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:signaling receptor complex adaptor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="C-Jun-amino-terminal kinase-interacting protein 3 isoform X1"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.20.5.1000:FF:000001">
        <property type="entry name" value="C-Jun-amino-terminal kinase-interacting protein 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000334">
        <property type="entry name" value="C-Jun-amino-terminal kinase-interacting protein 3 isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1000">
        <property type="entry name" value="arf6 gtpase in complex with a specific effector, jip4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039911">
        <property type="entry name" value="JIP3/JIP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032486">
        <property type="entry name" value="JIP_LZII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034743">
        <property type="entry name" value="RH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034744">
        <property type="entry name" value="RH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13886:SF3">
        <property type="entry name" value="C-JUN-AMINO-TERMINAL KINASE-INTERACTING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13886">
        <property type="entry name" value="JNK/SAPK-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16471">
        <property type="entry name" value="JIP_LZII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09744">
        <property type="entry name" value="RH1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40_2"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="11 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="21" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="21" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RH1" evidence="18">
        <location>
            <begin position="8"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="RH2" evidence="19">
        <location>
            <begin position="516"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="179"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="249"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
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            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="745"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1304"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="62"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="412"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="258"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="302"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="698"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="765"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="915"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="932"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1307"/>
            <end position="1317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009866"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00071163"/>
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    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00076107"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51776"/>
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    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51777"/>
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    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_047018446.2"/>
        </source>
    </evidence>
    <sequence length="1344" mass="148512" checksum="17EBA9AB7A6332F0" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QWJ0</accession>
    <name>A0A9F7QWJ0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Protein NLRC3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC128628652</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
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            <taxon>Siluriformes</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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            <tissue evidence="11">Blood</tissue>
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        <source>
            <dbReference type="Pfam" id="PF17779"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
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        <source>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
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                <person name="Geng X."/>
                <person name="Song L."/>
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                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
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                <person name="Yuan Z."/>
                <person name="Yang Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="6">Blood</tissue>
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        <source>
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        <source>
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        <source>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053538235.1">
        <property type="nucleotide sequence ID" value="XM_053682260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R3M7"/>
    <dbReference type="GeneID" id="128633473"/>
    <dbReference type="KEGG" id="ipu:128633473"/>
    <dbReference type="OrthoDB" id="8934837at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039809">
        <property type="entry name" value="Chemokine_b/g/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015:SF183">
        <property type="entry name" value="C-C MOTIF CHEMOKINE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015">
        <property type="entry name" value="SMALL INDUCIBLE CYTOKINE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00199">
        <property type="entry name" value="SCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0202">Cytokine</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039937819" evidence="5">
        <location>
            <begin position="22"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="6">
        <location>
            <begin position="29"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053538235.1"/>
        </source>
    </evidence>
    <sequence length="96" mass="11055" checksum="97DB3C16588A8C24" modified="2023-06-28" version="1">MRNLTALLFLLSLCSLQLVSSVPTIFNHITQCCTGTSKWKIPLKNIVTYWWTSNNCSIKAIVFETLNNGKHYRCVDPTAVWVNNHMKTVDRKNKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R4P6</accession>
    <name>A0A9F7R4P6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribonuclease inhibitor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC128632515</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053535356.1">
        <property type="nucleotide sequence ID" value="XM_053679381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R4P6"/>
    <dbReference type="GeneID" id="128632515"/>
    <dbReference type="KEGG" id="ipu:128632515"/>
    <dbReference type="OrthoDB" id="8956055at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053535356.1"/>
        </source>
    </evidence>
    <sequence length="86" mass="9590" checksum="ACF4BEE1D332F8B2" modified="2023-06-28" version="1">MWNCSITDEGCAALASAPRSNSSSHLRELNLNGNNPGKSGVKLLSDLLKDPHCNLETLHINYNKLTRTAYDRSLTSNLFSRIKQSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R561</accession>
    <name>A0A9F7R561_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">60S ribosomal protein L37a-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC128629454</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL43 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053533602.1">
        <property type="nucleotide sequence ID" value="XM_053677627.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R561"/>
    <dbReference type="GeneID" id="128629454"/>
    <dbReference type="KEGG" id="ipu:128629454"/>
    <dbReference type="OrthoDB" id="10316985at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48188">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48188:SF5">
        <property type="entry name" value="RIBOSOMAL PROTEIN EL43-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053533602.1"/>
        </source>
    </evidence>
    <sequence length="100" mass="11352" checksum="541003D4622BA0EE" modified="2023-06-28" version="1">MYKKAKCTKKVGIVVKYGTGYGTSLRKMVEKIEISQHGKYACSFCGKTRMKKKVVGLRHFESCMKTFDGGVWTCNITLAVIVESAIKRLKEMKGFIFLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R5I1</accession>
    <name>A0A9F7R5I1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein LOC128628675 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC128628675</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053530488.1">
        <property type="nucleotide sequence ID" value="XM_053674513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R5I1"/>
    <dbReference type="GeneID" id="128628675"/>
    <dbReference type="KEGG" id="ipu:128628675"/>
    <dbReference type="Proteomes" id="UP000221080">
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    <evidence type="ECO:0000313" key="4">
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</entry>
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    <accession>A0A9F7R8Q3</accession>
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        <recommendedName>
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        <name type="scientific">Ictalurus punctatus</name>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="26">SDA103</strain>
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    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="27">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="23">Receptor for glutamate that functions as a ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.</text>
    </comment>
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        <reaction evidence="19">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
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    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18 23">Postsynaptic cell membrane</location>
            <topology evidence="18 23">Multi-pass membrane protein</topology>
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    </comment>
    <comment type="similarity">
        <text evidence="23">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
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        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Lig_chan"/>
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    </dbReference>
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        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NMDARECEPTOR"/>
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        <property type="entry name" value="Lig_chan-Glu_bd"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Voltage-gated potassium channels"/>
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    </dbReference>
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    <keyword evidence="8 23" id="KW-0406">Ion transport</keyword>
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    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0564">Palmitate</keyword>
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    <keyword evidence="12 23 27" id="KW-0675">Receptor</keyword>
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    <feature type="signal peptide" evidence="23">
        <location>
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        </location>
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            <begin position="24"/>
            <end position="926"/>
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        <location>
            <begin position="541"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="623"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="813"/>
            <end position="835"/>
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    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="24">
        <location>
            <begin position="414"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="25">
        <location>
            <begin position="424"/>
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    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="498"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="500"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="505"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="673"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="674"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="724"/>
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        <ligand>
            <name>L-glutamate</name>
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        </ligand>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="21">
        <location>
            <position position="473"/>
        </location>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="21">
        <location>
            <position position="652"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="21">
        <location>
            <position position="771"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
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            <end position="330"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="737"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU367118"/>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_053538285.1"/>
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    </evidence>
    <sequence length="926" mass="103928" checksum="723D171C2E89E8C2" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R8Y6</accession>
    <name>A0A9F7R8Y6_ICTPU</name>
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            <fullName evidence="15">Lysosomal dipeptide transporter MFSD1</fullName>
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        <alternativeName>
            <fullName evidence="16">Major facilitator superfamily domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
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        <name evidence="22" type="primary">mfsd1l</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
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        </lineage>
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    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Lysosomal dipeptide uniporter that selectively exports lysine, arginine or histidine-containing dipeptides with a net positive charge from the lysosome lumen into the cytosol. Could play a role in a specific type of protein O-glycosylation indirectly regulating macrophages migration and tissue invasion. Also essential for liver homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-alanyl-L-lysine(out) = L-alanyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79415"/>
            <dbReference type="ChEBI" id="CHEBI:192470"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-alpha-aminoacyl-L-arginine(out) = L-alpha-aminoacyl-L-arginine(in)</text>
            <dbReference type="Rhea" id="RHEA:79367"/>
            <dbReference type="ChEBI" id="CHEBI:229968"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-alpha-aminoacyl-L-histidine(out) = L-alpha-aminoacyl-L-histidine(in)</text>
            <dbReference type="Rhea" id="RHEA:79375"/>
            <dbReference type="ChEBI" id="CHEBI:229967"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-alpha-aminoacyl-L-lysine(out) = L-alpha-aminoacyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79383"/>
            <dbReference type="ChEBI" id="CHEBI:229966"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-arginyl-L-alpha-amino acid(out) = L-arginyl-L-alpha-amino acid(in)</text>
            <dbReference type="Rhea" id="RHEA:79371"/>
            <dbReference type="ChEBI" id="CHEBI:84315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-arginyl-glycine(out) = L-arginyl-glycine(in)</text>
            <dbReference type="Rhea" id="RHEA:79391"/>
            <dbReference type="ChEBI" id="CHEBI:229955"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-aspartyl-L-lysine(out) = L-aspartyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79411"/>
            <dbReference type="ChEBI" id="CHEBI:229953"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-histidyl-L-alpha-amino acid(out) = L-histidyl-L-alpha-amino acid(in)</text>
            <dbReference type="Rhea" id="RHEA:79379"/>
            <dbReference type="ChEBI" id="CHEBI:229964"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>L-histidyl-glycine(out) = L-histidyl-glycine(in)</text>
            <dbReference type="Rhea" id="RHEA:79395"/>
            <dbReference type="ChEBI" id="CHEBI:229957"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-lysyl-L-alanine(out) = L-lysyl-L-alanine(in)</text>
            <dbReference type="Rhea" id="RHEA:79399"/>
            <dbReference type="ChEBI" id="CHEBI:229954"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-lysyl-L-alpha-amino acid(out) = L-lysyl-L-alpha-amino acid(in)</text>
            <dbReference type="Rhea" id="RHEA:79387"/>
            <dbReference type="ChEBI" id="CHEBI:229965"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-lysyl-L-lysine(out) = L-lysyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79403"/>
            <dbReference type="ChEBI" id="CHEBI:229956"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-lysyl-glycine(out) = L-lysyl-glycine(in)</text>
            <dbReference type="Rhea" id="RHEA:79407"/>
            <dbReference type="ChEBI" id="CHEBI:191202"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="18">Homodimer. Interacts with lysosomal protein GLMP (via lumenal domain); the interaction starts while both proteins are still in the endoplasmic reticulum and is required for stabilization of MFSD1 in lysosomes but has no direct effect on its targeting to lysosomes or transporter activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053540800.1">
        <property type="nucleotide sequence ID" value="XM_053684825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R8Y6"/>
    <dbReference type="GeneID" id="108271844"/>
    <dbReference type="KEGG" id="ipu:108271844"/>
    <dbReference type="CTD" id="100003326"/>
    <dbReference type="OrthoDB" id="424834at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052187">
        <property type="entry name" value="MFSD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512:SF5">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="313"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="411"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="20">
        <location>
            <begin position="11"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00044876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00044878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00045018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00046376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_053540800.1"/>
        </source>
    </evidence>
    <sequence length="452" mass="50200" checksum="D764443FEF430BEB" modified="2023-06-28" version="1">MAKPAERAYYRFLVLFFNCMLTFGSYFCFDIPSVLQQQFHGNLTCVNSTLGNETVCVEGLGMTAQEYNLLYAVYAWTNAVVVIMAGFLIDKLGNRFGVFLFSFLCVLGSSIFALGSHFKGSPYLLPLMLMGRLLFGSGNGSLTIVQNRITVFWFRGKELALAFGLTLAFSRLGSILNFFLTHRFEAQYGMQWTLWGGTLLCVLGFLSAIMVSVLDKVGMKQLGLEGTIQEESRKVRVQDVRLLCLRYWLLVFTIMFFYNGIFPFIADASKFIQDKYEGYTQKQAAYTVGAVYDSSLVLLAAVGILIDYVGLRGVFAVLCAVCTLPVFGLLAFTFVPPLVSTIWLGITYSFAAASMWPSIPLVVPRSTLGTAMGLATSVQMMGVGLCNLIVGEILGVENSDVKIPLWRWQRMMIFMLANTVGCIITSTVLNIIDWRKGGTLNKMTKRSDLQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RA65</accession>
    <name>A0A9F7RA65_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Tetratricopeptide repeat protein 33 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ttc33</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053532931.1">
        <property type="nucleotide sequence ID" value="XM_053676956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RA65"/>
    <dbReference type="GeneID" id="108260267"/>
    <dbReference type="CTD" id="23548"/>
    <dbReference type="OrthoDB" id="3263820at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:DNA transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057667">
        <property type="entry name" value="HTH_SB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052338">
        <property type="entry name" value="Transposase_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002492">
        <property type="entry name" value="Transposase_Tc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022:SF135">
        <property type="entry name" value="SI:DKEY-77F5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23022">
        <property type="entry name" value="TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25787">
        <property type="entry name" value="HTH_SB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01498">
        <property type="entry name" value="HTH_Tnp_Tc3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="29"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Transposase Tc1-like" evidence="2">
        <location>
            <begin position="94"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053532931.1"/>
        </source>
    </evidence>
    <sequence length="277" mass="31055" checksum="F28821D6516F57A0" modified="2023-06-28" version="1">MGSRCVKFGVSALTLPHTGDWKFNMAPHGKELSVDLKKRIVTLHKDGLGYKKFAKTLTLSCSTVAKTIQQFNSTGSTQNRPRHGRPKKSSAPAQRHIQRLSLGNRRMSAASIAAEVEGVVGQPVSAQTIRRTLHQIGVYGCRPRRKPLLKMMHKKARKQFVEDKQTKDMDYWNKVLWSDQTKINLFGSDGVKHVWWQPGEEYKDKCVLPTVKHGGGSVMVWGCMSAAGTGELQFIEGTMNADMNCDILKQSMISIHYSSMITTPNTPPRRPQPCYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RAJ8</accession>
    <name>A0A9F7RAJ8_ICTPU</name>
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        <submittedName>
            <fullName evidence="15">CREB-regulated transcription coactivator 1b isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">crtc1b</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TORC family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_053683255.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="108271387"/>
    <dbReference type="KEGG" id="ipu:108271387"/>
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        <property type="component" value="Chromosome 10"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051289">
        <property type="term" value="P:protein homotetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="TORC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024785">
        <property type="entry name" value="TORC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024784">
        <property type="entry name" value="TORC_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024783">
        <property type="entry name" value="TORC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13589">
        <property type="entry name" value="CREB-REGULATED TRANSCRIPTION COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13589:SF14">
        <property type="entry name" value="CREB-REGULATED TRANSCRIPTION COACTIVATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12886">
        <property type="entry name" value="TORC_C"/>
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    </dbReference>
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        <property type="entry name" value="TORC_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12884">
        <property type="entry name" value="TORC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transducer of regulated CREB activity N-terminal" evidence="11">
        <location>
            <begin position="6"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Transducer of regulated CREB activity middle" evidence="12">
        <location>
            <begin position="144"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Transducer of regulated CREB activity C-terminal" evidence="13">
        <location>
            <begin position="518"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="105"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="182"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="247"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="382"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="185"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="309"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="382"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="403"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007167"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF12884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF12885"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF12886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_053539230.1"/>
        </source>
    </evidence>
    <sequence length="593" mass="63977" checksum="E0AB27CFC30D21CC" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RCX3</accession>
    <name>A0A9F7RCX3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11 12">NLR family CARD domain-containing protein 3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 11 12" type="primary">LOC128634208</name>
    </gene>
    <organism evidence="9 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11 12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11 12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053540458.1">
        <property type="nucleotide sequence ID" value="XM_053684483.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053540459.1">
        <property type="nucleotide sequence ID" value="XM_053684484.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053540460.1">
        <property type="nucleotide sequence ID" value="XM_053684485.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A9F7RCX3"/>
    <dbReference type="GeneID" id="128634208"/>
    <dbReference type="KEGG" id="ipu:128634208"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Si:dkey-11n14.1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Si:dkey-16p6.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="8">
        <location>
            <begin position="229"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053540458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053540459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053540460.1"/>
        </source>
    </evidence>
    <sequence length="918" mass="104369" checksum="682AD8198DFE7017" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RH66</accession>
    <name>A0A9F7RH66_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Transmembrane protein 237A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">tmem237a</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the transition zone in primary cilia. Required for ciliogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TMEM237 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053536651.1">
        <property type="nucleotide sequence ID" value="XM_053680676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RH66"/>
    <dbReference type="GeneID" id="108266264"/>
    <dbReference type="KEGG" id="ipu:108266264"/>
    <dbReference type="CTD" id="557237"/>
    <dbReference type="OrthoDB" id="550113at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029409">
        <property type="entry name" value="TMEM237"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28388">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28388:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15383">
        <property type="entry name" value="TMEM237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="34"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_053536651.1"/>
        </source>
    </evidence>
    <sequence length="375" mass="42487" checksum="CA88E801C799008E" modified="2023-06-28" version="1">MLITKSKKRKAKKEINGLKEPEKETCLESTVEMEELVSCNPSGSMDARSPEPQDIPPQKKKKKSKKMAHTFAMESEQQDLVNGDTQMSPTDRKEVTRKSRRKRKSKITENQHNNSVDVEDDDVVTDAHTSISHRSLFSTPLGHNQSLSKVFVERSLRFQATDRLDLGHEQVKELLEVGSMWNSRDVALRVHSGFRLIGLFCHGFLAGCAIWNIIVIYVLAGEQLKTLSNLLQQYHSLAYPTQSLLYFLLAISSVSAFDRVNLAKASMALRGFLMLDPAALASFLYFAALILSLSQQMTSDRINLYLIANESLWPPGSEHQILQPWIVVNLVVALLVGLAWFFISTRPDMDYTEEFLISMEVDDYLQYKDKSEIPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RI63</accession>
    <name>A0A9F7RI63_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">DNA replication ATP-dependent helicase/nuclease</fullName>
            <ecNumber evidence="21">3.1.-.-</ecNumber>
            <ecNumber evidence="21">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">dna2</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">SDA103</strain>
        </source>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="28">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Key enzyme involved in DNA replication and DNA repair. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair by mediating the cleavage of 5'-ssDNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20 21">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="21">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 21">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="21"/>
    <dbReference type="EC" id="3.6.4.12" evidence="21"/>
    <dbReference type="RefSeq" id="XP_053535782.1">
        <property type="nucleotide sequence ID" value="XM_053679807.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108263385"/>
    <dbReference type="KEGG" id="ipu:108263385"/>
    <dbReference type="CTD" id="1763"/>
    <dbReference type="OrthoDB" id="306218at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033567">
        <property type="term" value="P:DNA replication, Okazaki fragment processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071932">
        <property type="term" value="P:replication fork reversal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18041">
        <property type="entry name" value="DEXXQc_DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22318">
        <property type="entry name" value="DNA2_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000721">
        <property type="entry name" value="DNA replication ATP-dependent helicase/nuclease DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000789">
        <property type="entry name" value="DNA replication ATP-dependent helicase/nuclease DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000915">
        <property type="entry name" value="DNA replication ATP-dependent helicase/nuclease DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026851">
        <property type="entry name" value="Dna2/JHS1_DEXXQ-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048459">
        <property type="entry name" value="DNA2_Rift"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014808">
        <property type="entry name" value="DNA_replication_fac_Dna2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF433">
        <property type="entry name" value="DNA REPLICATION ATP-DEPENDENT HELICASE_NUCLEASE DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08696">
        <property type="entry name" value="Dna2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21123">
        <property type="entry name" value="Dna2_Rift"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 21" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="12 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 21" id="KW-0227">DNA damage</keyword>
    <keyword evidence="17 21" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 21" id="KW-0235">DNA replication</keyword>
    <keyword evidence="15 21" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11 21 28" id="KW-0347">Helicase</keyword>
    <keyword evidence="10 21" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 21" id="KW-0408">Iron</keyword>
    <keyword evidence="14 21" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16 21" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="19 21" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5 21" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18 21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication factor Dna2 N-terminal" evidence="23">
        <location>
            <begin position="405"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2 rift barrel" evidence="26">
        <location>
            <begin position="803"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="24">
        <location>
            <begin position="960"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="24">
        <location>
            <begin position="1063"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="25">
        <location>
            <begin position="1138"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="69"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="222"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="89"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="165"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU367041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF08696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF21123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_053535782.1"/>
        </source>
    </evidence>
    <sequence length="1388" mass="155256" checksum="CE2E4FAC6734B1BC" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RLX4</accession>
    <name>A0A9F7RLX4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">C-terminal PDZ ligand of neuronal nitric oxide synthase protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Nitric oxide synthase 1 adaptor protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">nos1apb</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Adapter protein involved in neuronal nitric-oxide (NO) synthesis regulation via its association with nNOS/NOS1. The complex formed with NOS1 and synapsins is necessary for specific NO and synapsin functions at a presynaptic level. Mediates an indirect interaction between NOS1 and RASD1 leading to enhance the ability of NOS1 to activate RASD1. Competes with DLG4 for interaction with NOS1, possibly affecting NOS1 activity by regulating the interaction between NOS1 and DLG4. In kidney podocytes, plays a role in podosomes and filopodia formation through CDC42 activation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053537758.1">
        <property type="nucleotide sequence ID" value="XM_053681783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RLX4"/>
    <dbReference type="GeneID" id="108267139"/>
    <dbReference type="KEGG" id="ipu:108267139"/>
    <dbReference type="CTD" id="100331166"/>
    <dbReference type="OrthoDB" id="10030336at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050998">
        <property type="term" value="F:nitric-oxide synthase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000124">
        <property type="entry name" value="carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051133">
        <property type="entry name" value="Adapter_Engulfment-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232:SF76">
        <property type="entry name" value="CARBOXYL-TERMINAL PDZ LIGAND OF NEURONAL NITRIC OXIDE SYNTHASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232">
        <property type="entry name" value="PHOSPHOTYROSINE INTERACTION DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PID" evidence="8">
        <location>
            <begin position="20"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="171"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="336"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="215"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="172"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="344"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00054402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00067706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00075003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00075107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053537758.1"/>
        </source>
    </evidence>
    <sequence length="406" mass="46190" checksum="B0BFFC2E2FFBC98C" modified="2023-06-28" version="1">MPEKTKYNLVDDSDDSFQCGIAFQVKFIGTLDIVRPQSRLDILAAMRRVRYEFKVKNIKKMKVSLVVAVDGVKVVLHKKKKKNAYSWDESHVTLAQDPIYRIFYVSHDSQDLKIFSYIAKDKKSNVFRCNVFKSKRKSQAMRIVQTVGQAFEVCHKQSMDTVDEWLVKCEEERAEEEDTPDADTNPEETMQNTDSSEDAVVEIEPSGLLSPVPERLSLNHRVQLLKKQLKQQEQKSQAASSQAALLQQQLCLETQARTEAQVRAQQLLQQNGELLQHLSMLIKHIQELELRTHSSSSTSELSPLGSQDSLLEIALRDNVPRFSPLKKPNVVRLDPFGFFPEPSDSEKKPEHDRDSGQGPDEEQSESSPTEAHFFCTLELPGFRESGIASGYESNTDESDDRESWGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RMJ7</accession>
    <name>A0A9F7RMJ7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Circumsporozoite protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC128628720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
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                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.</text>
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        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 19">Cell membrane</location>
            <topology evidence="1 19">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053529758.1">
        <property type="nucleotide sequence ID" value="XM_053673783.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A9F7TCL0"/>
    <dbReference type="GeneID" id="108280231"/>
    <dbReference type="CTD" id="568370"/>
    <dbReference type="OrthoDB" id="6252479at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
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    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:catenin complex"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:adherens junction organization"/>
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        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:synaptic membrane adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="5"/>
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        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000009">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000012">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000014">
        <property type="entry name" value="Cadherin 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000097">
        <property type="entry name" value="cadherin-12 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="5"/>
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        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
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    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
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        <property type="entry name" value="Cadherin-like_sf"/>
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        <property type="entry name" value="Cadherin_CS"/>
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        <property type="entry name" value="Cadherin_Y-type_LIR"/>
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    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF322">
        <property type="entry name" value="CADHERIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01049">
        <property type="entry name" value="CADH_Y-type_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="5"/>
    </dbReference>
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    <keyword evidence="9 18 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 19" id="KW-0130">Cell adhesion</keyword>
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    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
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    <keyword evidence="7 21" id="KW-0732">Signal</keyword>
    <keyword evidence="5 19 20" id="KW-0812">Transmembrane</keyword>
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        <location>
            <begin position="1"/>
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        <location>
            <begin position="20"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="631"/>
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        </location>
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    <feature type="domain" description="Cadherin" evidence="22">
        <location>
            <begin position="68"/>
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    <feature type="domain" description="Cadherin" evidence="22">
        <location>
            <begin position="149"/>
            <end position="257"/>
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    </feature>
    <feature type="domain" description="Cadherin" evidence="22">
        <location>
            <begin position="258"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="22">
        <location>
            <begin position="373"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="22">
        <location>
            <begin position="508"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037319"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00041042"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00069585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_053529758.1"/>
        </source>
    </evidence>
    <sequence length="808" mass="90239" checksum="5645B43BE5A48604" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TFF9</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein LOC128628718</fullName>
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    <gene>
        <name evidence="2" type="primary">LOC128628718</name>
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    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
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    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SDA103</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053533343.1">
        <property type="nucleotide sequence ID" value="XM_053677368.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A9F7TFF9"/>
    <dbReference type="GeneID" id="128628718"/>
    <dbReference type="KEGG" id="ipu:128628718"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36493:SF3">
        <property type="entry name" value="CHITIN-BINDING TYPE-4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36493">
        <property type="entry name" value="NEUROBLAST DIFFERENTIATION-ASSOCIATED PROTEIN AHNAK-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_053533343.1"/>
        </source>
    </evidence>
    <sequence length="539" mass="65924" checksum="7A9C5802FC7A9656" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TFZ6</accession>
    <name>A0A9F7TFZ6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serpin B6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 12 13 14" type="primary">LOC108261526</name>
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    <organism evidence="10 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11 12 13" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11 12 13">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Forms a complex with the monomeric form of beta-tryptase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the serpin family. Ov-serpin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053531810.1">
        <property type="nucleotide sequence ID" value="XM_053675835.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053531811.1">
        <property type="nucleotide sequence ID" value="XM_053675836.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053531812.1">
        <property type="nucleotide sequence ID" value="XM_053675837.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053531813.1">
        <property type="nucleotide sequence ID" value="XM_053675838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TFZ6"/>
    <dbReference type="GeneID" id="108261526"/>
    <dbReference type="KEGG" id="ipu:108261526"/>
    <dbReference type="OrthoDB" id="671595at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19956">
        <property type="entry name" value="serpinB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.39.10:FF:000014">
        <property type="entry name" value="Serpin family B member 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.580">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF204">
        <property type="entry name" value="SERPIN B6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0722">Serine protease inhibitor</keyword>
    <feature type="domain" description="Serpin" evidence="9">
        <location>
            <begin position="13"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053531810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053531811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_053531812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_053531813.1"/>
        </source>
    </evidence>
    <sequence length="417" mass="47216" checksum="28D67B88BE375255" modified="2023-06-28" version="1">MESLSVANTNFALHLFTKIKEGNKTGNVFYSPLSISSALAMVSLGAAGNTATQMSEVLCFNKPVKPKSVQPVAVQQAQQAQKVQLPSALPKVLHHNKAKDNVHVSFNKLMAELNKAGAPYALSTANRLYAEQTYKFVEKFLKETKTHYHAELETVDFKANAESARVNINNWVEKQTKDKIKNLLEKGAVDNSTRLVLVNAIYFKGSWEEQFKVETTQERPFKLNKKETKPVQMMNQEAEFPLAFIPDLRCKILEMPYKGMELSMLIMLPDEIEDNTTGLEKLERQLTYDNFMEWTRPDMMDTVQVQVSLPRFKLEKTYDMNELLISMGMVDAFDKGKCDFSHMSPCDDLVLSEVVHKSFVEVNEEGTEAAAATATGMLTCCLTPPRKERFVADHPFLFFIQHKPTRSILFCGRYSAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TIQ5</accession>
    <name>A0A9F7TIQ5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">NACHT, LRR and PYD domains-containing protein 12-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">LOC128629617</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053534330.1">
        <property type="nucleotide sequence ID" value="XM_053678355.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534331.1">
        <property type="nucleotide sequence ID" value="XM_053678356.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TIQ5"/>
    <dbReference type="GeneID" id="128629617"/>
    <dbReference type="KEGG" id="ipu:128629617"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00116">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000782">
        <property type="entry name" value="Si:ch211-196h16.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000210">
        <property type="entry name" value="Si:dkey-16p6.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001632">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="8">
        <location>
            <begin position="229"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053534330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053534331.1"/>
        </source>
    </evidence>
    <sequence length="1054" mass="119270" checksum="489542A2565CFAD3" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TKC5</accession>
    <name>A0A9F7TKC5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC128632871</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC128632871</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
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    </reference>
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                <consortium name="RefSeq"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_053680547.1"/>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053536522.1"/>
        </source>
    </evidence>
    <sequence length="315" mass="34896" checksum="D9A236F53C085087" modified="2023-06-28" version="1">MKRDYTDHKPGHLHSTPPLGKSPPRPDGHAMAALLLARAPGSKKSGTQEDEGVRATENTDFRSYAHDPAVMCRYEQQGALDQVLFYGMMHYIGNKIIAPPPYEVHYIGNKIIAPPPYEVHYIGNKIIAPPPYEVHYIGNKIIAPPPYEVHYIGNKIIAPPPCEVHYIGNKMIAPPPYEVHYIGNKIIAPPPCEVHYIGNKIIAPPPCEVHYIGNKIIAPPPCEVHYIGNKIIAPPPCEVHYIGNKMIAPPPYEVHYIGNKIIAPPPCEVHYIGNKIIAPPPCEVHYIGNKIIAPPPCEVHYIGNRIIAPPPCEVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TM72</accession>
    <name>A0A9F7TM72_ICTPU</name>
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        <submittedName>
            <fullName evidence="10">NACHT, LRR and PYD domains-containing protein 3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC108273219</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053540438.1">
        <property type="nucleotide sequence ID" value="XM_053684463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TM72"/>
    <dbReference type="GeneID" id="108273219"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
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        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00116">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000210">
        <property type="entry name" value="Si:dkey-16p6.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001632">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
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        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="8">
        <location>
            <begin position="230"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_053540438.1"/>
        </source>
    </evidence>
    <sequence length="1030" mass="115862" checksum="A92E665605D51E8C" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="9">Regulator of G-protein signaling 14a isoform X1</fullName>
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    </protein>
    <gene>
        <name evidence="9" type="primary">rgs14a</name>
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    <organism evidence="8 9">
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053541741.1">
        <property type="nucleotide sequence ID" value="XM_053685766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TQR4"/>
    <dbReference type="GeneID" id="108275341"/>
    <dbReference type="CTD" id="327368"/>
    <dbReference type="OrthoDB" id="196547at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008277">
        <property type="term" value="P:regulation of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007051">
        <property type="term" value="P:spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17139">
        <property type="entry name" value="RBD2_RGS14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000001">
        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.196.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003109">
        <property type="entry name" value="GoLoco_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046993">
        <property type="entry name" value="RBD2_RGS14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003116">
        <property type="entry name" value="RBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046995">
        <property type="entry name" value="RGS10/12/14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024066">
        <property type="entry name" value="RGS_subdom1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45945:SF2">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45945">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING LOCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02188">
        <property type="entry name" value="GoLoco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02196">
        <property type="entry name" value="RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00390">
        <property type="entry name" value="GoLoco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00455">
        <property type="entry name" value="RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50877">
        <property type="entry name" value="GOLOCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50898">
        <property type="entry name" value="RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RGS" evidence="6">
        <location>
            <begin position="87"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="RBD" evidence="7">
        <location>
            <begin position="310"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="RBD" evidence="7">
        <location>
            <begin position="389"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="40"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="216"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="277"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="523"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="217"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="277"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="297"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="525"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="546"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="566"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_053541741.1"/>
        </source>
    </evidence>
    <sequence length="576" mass="63624" checksum="A18EDDA8BD5DE3A4" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>E3TDW1</accession>
    <name>E3TDW1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12 15">Uricase</fullName>
            <ecNumber evidence="5 12 15">1.7.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">Urate oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">URIC</name>
        <name evidence="18" type="synonym">uox</name>
        <name evidence="18" type="synonym">uric</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 18" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 12 15">Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12 15">
            <text>urate + O2 + H2O = 5-hydroxyisourate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:21368"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
            <dbReference type="EC" id="1.7.3.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Purine metabolism; urate degradation; (S)-allantoin from urate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12 15">Belongs to the uricase family.</text>
    </comment>
    <dbReference type="EC" id="1.7.3.3" evidence="5 12 15"/>
    <dbReference type="EMBL" id="GU588541">
        <property type="protein sequence ID" value="ADO28497.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187325.1">
        <property type="nucleotide sequence ID" value="NM_001200396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TDW1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000004695"/>
    <dbReference type="GeneID" id="100528126"/>
    <dbReference type="KEGG" id="ipu:100528126"/>
    <dbReference type="CTD" id="391051"/>
    <dbReference type="OrthoDB" id="9992118at2759"/>
    <dbReference type="UniPathway" id="UPA00394">
        <property type="reaction ID" value="UER00650"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004846">
        <property type="term" value="F:urate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006145">
        <property type="term" value="P:purine nucleobase catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019628">
        <property type="term" value="P:urate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.270.10:FF:000001">
        <property type="entry name" value="Uricase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.270.10">
        <property type="entry name" value="Urate Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002042">
        <property type="entry name" value="Uricase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03383">
        <property type="entry name" value="urate_oxi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42874">
        <property type="entry name" value="URICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42874:SF1">
        <property type="entry name" value="URICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01014">
        <property type="entry name" value="Uricase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000241">
        <property type="entry name" value="Urate_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00093">
        <property type="entry name" value="URICASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 12 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9 12" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7 12 15" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Charge relay system" evidence="13">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="13">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="13">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>O2</name>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>O2</name>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000241-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000241-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ADO28497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="NP_001187325.1"/>
        </source>
    </evidence>
    <sequence length="298" mass="34228" checksum="23491D9D4EDB4E02" modified="2011-01-11" version="1">MTTSNQNVEFVRTGYGKNQVKVLYIRREGNQHYILELKADVQLTLNSLKDYLTGDNSDIIPTDTIKNTVHALAKLKGVKTIEGFALDICNHFLTAFKHVTRAKVNIEEAPWKRLEKNAVEHAHAFILSPESLHFCEVEQYRNANAVIHSGIKDMKVLKTTQSGFEGFLKDRFTTLQEAKDRVFCTSVYARWRYNQHKEVNFDMAWKTIKDSIIEKFAGPYDRGEYSPSVQKTLYDTQILVLERLPEVEEIEIIMPNQHYFTIDMTKLGLSNKDEVLLPLDNPAGNITGTIYRTPQAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>E3TE33</accession>
    <name>E3TE33_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">creatine kinase</fullName>
            <ecNumber evidence="2">2.7.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">KCRB</name>
        <name evidence="15" type="synonym">ckba</name>
        <name evidence="15" type="synonym">kcrb</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2016" name="Biol. Reprod." volume="95" first="19" last="19">
            <title>Transcriptome Display During Testicular Differentiation of Channel Catfish (Ictalurus punctatus) as Revealed by RNA-Seq Analysis.</title>
            <authorList>
                <person name="Zeng Q."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Zhang Y."/>
                <person name="Yuan Z."/>
                <person name="Jiang C."/>
                <person name="Chen A."/>
                <person name="Fu Q."/>
                <person name="Su B."/>
                <person name="Dunham R."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="27307075"/>
            <dbReference type="DOI" id="10.1095/biolreprod.116.138818"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>creatine + ATP = N-phosphocreatine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17157"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57947"/>
            <dbReference type="ChEBI" id="CHEBI:58092"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.3.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:17158"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 10">Belongs to the ATP:guanido phosphotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.3.2" evidence="2"/>
    <dbReference type="EMBL" id="GU588613">
        <property type="protein sequence ID" value="ADO28569.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187380.1">
        <property type="nucleotide sequence ID" value="NM_001200451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TE33"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005149"/>
    <dbReference type="GeneID" id="100528181"/>
    <dbReference type="KEGG" id="ipu:100528181"/>
    <dbReference type="CTD" id="564694"/>
    <dbReference type="OMA" id="ISSDWPY"/>
    <dbReference type="OrthoDB" id="430219at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004111">
        <property type="term" value="F:creatine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046314">
        <property type="term" value="P:phosphocreatine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00716">
        <property type="entry name" value="creatine_kinase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000026">
        <property type="entry name" value="Creatine kinase B-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.135.10:FF:000001">
        <property type="entry name" value="Creatine kinase M-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.135.10">
        <property type="entry name" value="ATP:guanido phosphotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000749">
        <property type="entry name" value="ATP-guanido_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022415">
        <property type="entry name" value="ATP-guanido_PTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022414">
        <property type="entry name" value="ATP-guanido_PTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022413">
        <property type="entry name" value="ATP-guanido_PTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036802">
        <property type="entry name" value="ATP-guanido_PTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547">
        <property type="entry name" value="ARGININE OR CREATINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547:SF23">
        <property type="entry name" value="CREATINE KINASE B-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00217">
        <property type="entry name" value="ATP-gua_Ptrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02807">
        <property type="entry name" value="ATP-gua_PtransN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48034">
        <property type="entry name" value="Guanido kinase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00112">
        <property type="entry name" value="PHOSPHAGEN_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51510">
        <property type="entry name" value="PHOSPHAGEN_KINASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51509">
        <property type="entry name" value="PHOSPHAGEN_KINASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphagen kinase N-terminal" evidence="11">
        <location>
            <begin position="11"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphagen kinase C-terminal" evidence="12">
        <location>
            <begin position="125"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="128"/>
            <end position="132"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="292"/>
            <end position="296"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="320"/>
            <end position="325"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ADO28569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="NP_001187380.1"/>
        </source>
    </evidence>
    <sequence length="381" mass="42730" checksum="4CCD9386856857DD" modified="2011-01-11" version="1">MPFGNTHNQLKMKYSSEAEFPDLTKHNNHMAKVLTPDLYERLRSKQTPSGFTLDDVIQTGVDNPGHPFIMTVGCVAGDEETYEVFKELLDPVIKDRHGGYKPTDKHKTDLNPANLKGGDDLDPNYVLSSRVRTGRSIRGFCLPPHCSRGERRAVEKLSVEALGALSGDLKGKYYALKNMSEAEQQQLIDDHFLFDKPVSPLLLASGMARDWPDARGIWHNDKKTFLVWVNEEDHLRVISMQKGGNMKEVFTRFCTGLTKIEELFKSKGHAFMWNEHLGYVLTCPSNLGTGLRGGVHVKLPNMSKHNKFEEVLKRLRLQKRGTGGVDTAAVGGVFDISNADRLGFSEVELVQMVVDGVKLLVEMEKRLEKGQAIDDLIPVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3TE67</accession>
    <name>E3TE67_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Src kinase-associated phosphoprotein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Src family-associated phosphoprotein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SKAP2</name>
        <name evidence="16" type="synonym">skap2</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 16" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">May be involved in B-cell and macrophage adhesion processes. May play a role in src signaling pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SKAP family.</text>
    </comment>
    <dbReference type="EMBL" id="GU588647">
        <property type="protein sequence ID" value="ADO28603.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001188121.1">
        <property type="nucleotide sequence ID" value="NM_001201192.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TE67"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000004552"/>
    <dbReference type="GeneID" id="100528922"/>
    <dbReference type="KEGG" id="ipu:100528922"/>
    <dbReference type="CTD" id="8935"/>
    <dbReference type="OrthoDB" id="243840at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042113">
        <property type="term" value="P:B cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000194">
        <property type="entry name" value="Putative src kinase-associated phosphoprotein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000097">
        <property type="entry name" value="Putative src kinase-associated phosphoprotein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037781">
        <property type="entry name" value="SKAP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15129:SF2">
        <property type="entry name" value="SRC KINASE-ASSOCIATED PHOSPHOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15129">
        <property type="entry name" value="SRC-ASSOCIATED ADAPTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0075">B-cell activation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="14 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PH" evidence="13">
        <location>
            <begin position="106"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="12">
        <location>
            <begin position="284"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="54"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ADO28603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="NP_001188121.1"/>
        </source>
    </evidence>
    <sequence length="346" mass="39669" checksum="0F723977D4E82188" modified="2011-01-11" version="1">MKAIPEDLTTLVSDLEAFLTEVLKGENLSKKAKDKRDTFIKRIKDVKASYPQDFKEKRDEEWDEVEEADSNNDGSPLHSERMDRDEETAYDGVQHAPPVAAQDLQAVFKSGYLEKRRKDHSFFGTEWQKRWCALSNHTFYYYGSEKDKQQKGEFNISGYAVKINNSLRKDSKKDCCFEISAPDKRVYQFCASSQKEAEEWVEQISFVIKDMEGIIPLEEEQDTYDDCVPLSAASIPAPMPAPIDDDIYEELPEEEMPHTNKPAPEPVSKPPVALPVTAVNKSTDYQNYFQGLWDCNGDQPDELSFKRGDAIYILSKEYDTFGWWVGEMKGVIGIVPKSYLTELYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3TFY8</accession>
    <name>E3TFY8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Metaxin-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Mitochondrial outer membrane import complex protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">MTX2</name>
        <name evidence="17" type="synonym">mtx2</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 17" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in transport of proteins into the mitochondrion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with MTX1/metaxin-1. Associates with the mitochondrial contact site and cristae organizing system (MICOS) complex, composed of at least MICOS10/MIC10, CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26 and QIL1/MIC13. This complex was also known under the names MINOS or MitOS complex. The MICOS complex associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2 (together described as components of the mitochondrial outer membrane sorting assembly machinery (SAM) complex) and DNAJC11, mitochondrial inner membrane protein TMEM11 and with HSPA9. The MICOS and SAM complexes together with DNAJC11 are part of a large protein complex spanning both membranes termed the mitochondrial intermembrane space bridging (MIB) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="GU589273">
        <property type="protein sequence ID" value="ADO29224.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187864.1">
        <property type="nucleotide sequence ID" value="NM_001200935.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TFY8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000028031"/>
    <dbReference type="GeneID" id="100528665"/>
    <dbReference type="KEGG" id="ipu:100528665"/>
    <dbReference type="CTD" id="10651"/>
    <dbReference type="OrthoDB" id="198787at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001401">
        <property type="term" value="C:SAM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03211">
        <property type="entry name" value="GST_C_Metaxin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03079">
        <property type="entry name" value="GST_N_Metaxin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000033">
        <property type="entry name" value="metaxin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033468">
        <property type="entry name" value="Metaxin_GST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050931">
        <property type="entry name" value="Mito_Protein_Transport_Metaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019564">
        <property type="entry name" value="Sam37/metaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12289">
        <property type="entry name" value="METAXIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12289:SF38">
        <property type="entry name" value="METAXIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17171">
        <property type="entry name" value="GST_C_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10568">
        <property type="entry name" value="Tom37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01180">
        <property type="entry name" value="SUF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Mitochondrial outer membrane transport complex Sam37/metaxin N-terminal" evidence="13">
        <location>
            <begin position="41"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Metaxin glutathione S-transferase" evidence="14">
        <location>
            <begin position="187"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00072180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF10568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ADO29224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="NP_001187864.1"/>
        </source>
    </evidence>
    <sequence length="275" mass="31234" checksum="8742382F80F8D643" modified="2011-01-11" version="1">MSLAAEAFVSQIAAAEPWPENATLYQPLREDQILLSDCASSLAVQAYLRMLGLPVRVRCRANAEYMSPSGKVPFIHVGNQVVSELGPIMQFTRAKGHSLSDSLDDVQRAEMKAYMELVNNMLLTAELYIQWCYQNTAAEITRPRYSSPYSWPLNHILAYQKQWEVRRKMNAIGWAGKSLEQVYEDVSQCCQALSQRLGTQPFFFNKQPTELDALVFGHLFTILTTRLTTDELSEKIKSFSNLLSFCRRIEQAYFESQDRDSQSGALDYSKGPSLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3TGE6</accession>
    <name>E3TGE6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="2 4 5">Cornifelin-like protein b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">CNFNB</name>
        <name evidence="4 5" type="synonym">cnfnb</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cornifelin family.</text>
    </comment>
    <dbReference type="EMBL" id="GU589432">
        <property type="protein sequence ID" value="ADO29382.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001188228.1">
        <property type="nucleotide sequence ID" value="NM_001201299.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017326482.1">
        <property type="nucleotide sequence ID" value="XM_017470993.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TGE6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034215"/>
    <dbReference type="GeneID" id="100529029"/>
    <dbReference type="KEGG" id="ipu:100529029"/>
    <dbReference type="CTD" id="100529029"/>
    <dbReference type="GeneTree" id="ENSGT00940000163701"/>
    <dbReference type="OMA" id="SICNDCI"/>
    <dbReference type="OrthoDB" id="1045822at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006461">
        <property type="entry name" value="PLAC_motif_containing"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01571">
        <property type="entry name" value="A_thal_Cys_rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15907">
        <property type="entry name" value="DUF614 FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04749">
        <property type="entry name" value="PLAC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADO29382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="NP_001188228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017326482.1"/>
        </source>
    </evidence>
    <sequence length="128" mass="14568" checksum="285C7A28B7EB9950" modified="2011-01-11" version="1">MAKMVVQQPQPVMMANTSYSNQWSSGIFDCCENVAECCFSFWCFPCFACSTSRKFGECLCLPMLDGYGLIPPITLAMRASMRQRYGIEGSICNDCIYSFFCLTCVWCQMSREMKARNHSVTLINSHTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3TGE8</accession>
    <name>E3TGE8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Complex I-AGGG</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">NADH-ubiquinone oxidoreductase AGGG subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">NDUB2</name>
        <name evidence="17" type="synonym">ndub2</name>
        <name evidence="17" type="synonym">ndufb2</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 17" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Complex I is composed of 45 different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFB2 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GU589434">
        <property type="protein sequence ID" value="ADO29384.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187976.1">
        <property type="nucleotide sequence ID" value="NM_001201047.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TGE8"/>
    <dbReference type="GeneID" id="100528777"/>
    <dbReference type="KEGG" id="ipu:100528777"/>
    <dbReference type="CTD" id="4708"/>
    <dbReference type="GeneTree" id="ENSGT00390000004044"/>
    <dbReference type="OrthoDB" id="6241903at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026627">
        <property type="entry name" value="NDUFB2_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15223:SF1">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15223">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE AGGG SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14813">
        <property type="entry name" value="NADH_B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="15" id="KW-0830">Ubiquinone</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ADO29384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="NP_001187976.1"/>
        </source>
    </evidence>
    <sequence length="100" mass="11593" checksum="A1D35DB9051ED6DB" modified="2011-01-11" version="1">MSALWGTGRVLRAGAHLCRRGPRLSVIRKAGGGPHIVAQYRQPPQITKHQKFRSELLSGFMWFWIMWHFWHDSDAVFGHFPWPNTDAWTNEELGIPPDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5U7S7</accession>
    <name>W5U7S7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="3">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">Ube2a</name>
        <name evidence="15" type="synonym">LOC108259060</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="3"/>
    <dbReference type="EMBL" id="JT407613">
        <property type="protein sequence ID" value="AHH38051.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017313683.1">
        <property type="nucleotide sequence ID" value="XM_017458194.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U7S7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020452"/>
    <dbReference type="GeneID" id="108259060"/>
    <dbReference type="KEGG" id="ipu:108259060"/>
    <dbReference type="GeneTree" id="ENSGT00940000155075"/>
    <dbReference type="OMA" id="QERANCT"/>
    <dbReference type="OrthoDB" id="9984419at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23790">
        <property type="entry name" value="UBCc_UBE2A_2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000062">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="12">
        <location>
            <begin position="4"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="10">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AHH38051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017313683.1"/>
        </source>
    </evidence>
    <sequence length="152" mass="17287" checksum="4F7BEE39A245D7FE" modified="2014-04-16" version="1">MSTPARRRLMRDFKRLQEDPPAGVSGAPSENNIMVWNAVIFGPEGTPFEDGTFKLTVEFTEEYPNKPPTVRFTSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWRDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5UA52</accession>
    <name>W5UA52_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Synaptotagmin-like protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SYTL5</name>
        <name evidence="16" type="synonym">sytl5</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">May act as Rab effector protein and play a role in vesicle trafficking. Binds phospholipids.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT409944">
        <property type="protein sequence ID" value="AHH38881.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017325509.1">
        <property type="nucleotide sequence ID" value="XM_017470020.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UA52"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035577"/>
    <dbReference type="GeneID" id="108266551"/>
    <dbReference type="KEGG" id="ipu:108266551"/>
    <dbReference type="CTD" id="94122"/>
    <dbReference type="OMA" id="VEDKRIX"/>
    <dbReference type="OrthoDB" id="195679at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070382">
        <property type="term" value="C:exocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042043">
        <property type="term" value="F:neurexin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04029">
        <property type="entry name" value="C2A_SLP-4_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04020">
        <property type="entry name" value="C2B_SLP_1-2-3-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000006">
        <property type="entry name" value="Synaptotagmin-like 5, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000107">
        <property type="entry name" value="Synaptotagmin-like 5, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000018">
        <property type="entry name" value="Synaptotagmin-like 5, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041282">
        <property type="entry name" value="FYVE_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010911">
        <property type="entry name" value="Rab_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037303">
        <property type="entry name" value="SLP-4/5_C2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043567">
        <property type="entry name" value="SYTL1-5_C2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45716">
        <property type="entry name" value="BITESIZE, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45716:SF6">
        <property type="entry name" value="SYNAPTOTAGMIN-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02318">
        <property type="entry name" value="FYVE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50916">
        <property type="entry name" value="RABBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RabBD" evidence="13">
        <location>
            <begin position="7"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="349"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="507"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="144"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="167"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="258"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="269"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AHH38881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017325509.1"/>
        </source>
    </evidence>
    <sequence length="672" mass="75202" checksum="7B6B3E9496F1A9EE" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5UCD0</accession>
    <name>W5UCD0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4 6">Kelch repeat and BTB domain-containing protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">KBTBD4</name>
        <name evidence="6" type="synonym">kbtbd4</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT411664">
        <property type="protein sequence ID" value="AHH39545.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017321330.1">
        <property type="nucleotide sequence ID" value="XM_017465841.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UCD0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034281"/>
    <dbReference type="GeneID" id="108264374"/>
    <dbReference type="KEGG" id="ipu:108264374"/>
    <dbReference type="CTD" id="55709"/>
    <dbReference type="GeneTree" id="ENSGT00940000158775"/>
    <dbReference type="OMA" id="CYVKPYV"/>
    <dbReference type="OrthoDB" id="2311693at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="CDD" id="cd18481">
        <property type="entry name" value="BACK_KBTBD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042884">
        <property type="entry name" value="KBTBD4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042950">
        <property type="entry name" value="KBTBD4_BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011498">
        <property type="entry name" value="Kelch_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47195">
        <property type="entry name" value="KELCH REPEAT AND BTB DOMAIN-CONTAINING PROTEIN 4"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47195:SF1">
        <property type="entry name" value="KELCH REPEAT AND BTB DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07646">
        <property type="entry name" value="Kelch_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="50"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AHH39545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017321330.1"/>
        </source>
    </evidence>
    <sequence length="523" mass="58768" checksum="6AA6C05B7601FB61" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5UDA1</accession>
    <name>W5UDA1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10 12">Tripartite motif-containing protein 65</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">Trim65</name>
        <name evidence="12" type="synonym">trim65</name>
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    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
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            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT412639">
        <property type="protein sequence ID" value="AHH39933.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017338879.1">
        <property type="nucleotide sequence ID" value="XM_017483390.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UDA1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008259"/>
    <dbReference type="GeneID" id="108273818"/>
    <dbReference type="KEGG" id="ipu:108273818"/>
    <dbReference type="CTD" id="201292"/>
    <dbReference type="GeneTree" id="ENSGT00940000162951"/>
    <dbReference type="OMA" id="ACTVNEC"/>
    <dbReference type="OrthoDB" id="5951542at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
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    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF5">
        <property type="entry name" value="E3 UBIQUITIN_ISG15 LIGASE TRIM25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
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        <source>
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        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
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                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
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                <person name="Scheffler B."/>
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                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
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                <person name="Xie Y."/>
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                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">SDA103</strain>
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        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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    <dbReference type="KEGG" id="ipu:108263036"/>
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        <property type="component" value="Chromosome 3"/>
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    <dbReference type="GO" id="GO:0005509">
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="entry name" value="ONCOPROTEIN-INDUCED TRANSCRIPT 3 PROTEIN"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <source>
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        <source>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
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    <accession>W5UIK0</accession>
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        <name evidence="12" type="primary">LSM14A</name>
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    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
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                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
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            <dbReference type="PubMed" id="23127152"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
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                <person name="Dunham R."/>
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                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
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                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LSM14 family.</text>
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    <dbReference type="AlphaFoldDB" id="W5UIK0"/>
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        <property type="component" value="Chromosome 14"/>
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    <dbReference type="GO" id="GO:0000932">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:stress granule assembly"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01736">
        <property type="entry name" value="LSm14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000006">
        <property type="entry name" value="Protein LSM14 homolog A isoform b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025762">
        <property type="entry name" value="DFDF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019050">
        <property type="entry name" value="FDF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025761">
        <property type="entry name" value="FFD_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025609">
        <property type="entry name" value="Lsm14-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025768">
        <property type="entry name" value="TFG_box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13586:SF2">
        <property type="entry name" value="PROTEIN LSM14 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13586">
        <property type="entry name" value="SCD6 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09532">
        <property type="entry name" value="FDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12701">
        <property type="entry name" value="LSM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01199">
        <property type="entry name" value="FDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01271">
        <property type="entry name" value="LSM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51512">
        <property type="entry name" value="DFDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51513">
        <property type="entry name" value="FFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51536">
        <property type="entry name" value="TFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="11">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="DFDF" evidence="8">
        <location>
            <begin position="247"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="FFD box profile" evidence="9">
        <location>
            <begin position="321"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="TFG box profile" evidence="10">
        <location>
            <begin position="340"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="138"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="278"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="362"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="FFD box" evidence="5">
        <location>
            <begin position="321"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="TFG box" evidence="6">
        <location>
            <begin position="340"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="160"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="190"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="232"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="247"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="370"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="394"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="417"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AHH41877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017340932.1"/>
        </source>
    </evidence>
    <sequence length="426" mass="46156" checksum="236ED6DCABB81FDE" modified="2014-04-16" version="1">MSGGTPYIGSKISLISKAEIRYEGILYTIDTENSTVALAKVRSFGTEDRPTDRPILPRDETYEYIIFRGSDIKDLTVCEPPKPSLPQDPAIVQSSMGSAAAPSAFQSGNSYGLFKQAPVSPYSQFAASPLVSPPFGTVGGRSSPALDPLQKSPPVEPSAPVTSSVRSTAPVSRRSPVTARLGTSAQKPLDTPEQRRGEGRSRLRSGLGHAAADTNVVHSGFQSAAAGPPGPRRGRGGGHRGRGRFPVRRDGPMKFEKDFDFESANAQFNKEEIDKEFQSKLKLKDEKPEKALNGEDKGDSGVDTQNSEGNADEEDPLGPNCYYDKSKSFFDNISCDDMRERRQTWAEERRMNAETFGVPFRHNRNRGGFRGRGGMGFRGGRGRGSSRGAFGAQHPGGSGFRGGYRGGQGGRQFSSFEYRKDNKVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5UJ65</accession>
    <name>W5UJ65_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma</fullName>
            <ecNumber evidence="13">2.7.1.149</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phosphatidylinositol 5-phosphate 4-kinase type II gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">pip4k2c</name>
        <name evidence="21" type="synonym">pip4k2cb</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + ATP = 1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83423"/>
            <dbReference type="ChEBI" id="CHEBI:84968"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:55993"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + GTP = 1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55964"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:83423"/>
            <dbReference type="ChEBI" id="CHEBI:84968"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:55965"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12280"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57795"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.149"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:12281"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.1.149" evidence="13"/>
    <dbReference type="EMBL" id="JT416911">
        <property type="protein sequence ID" value="AHH42189.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017306212.1">
        <property type="nucleotide sequence ID" value="XM_017450723.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UJ65"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003353"/>
    <dbReference type="GeneID" id="108255072"/>
    <dbReference type="KEGG" id="ipu:108255072"/>
    <dbReference type="CTD" id="100535634"/>
    <dbReference type="OMA" id="EANTMDY"/>
    <dbReference type="OrthoDB" id="20783at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016309">
        <property type="term" value="F:1-phosphatidylinositol-5-phosphate 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17305">
        <property type="entry name" value="PIPKc_PIP5KII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.800.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 5-phosphate 4-kinase type-2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF35">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 5-PHOSPHATE 4-KINASE TYPE-2 GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 16 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="18">
        <location>
            <begin position="37"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="292"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AHH42189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017306212.1"/>
        </source>
    </evidence>
    <sequence length="417" mass="48133" checksum="E8C9D3CEE02E4F1D" modified="2014-04-16" version="1">MASPKPGSVSNPPPSKLRRTKSKKKHFVHQKVEVFRASDPVLSVFMWGINHTVSDMVHVPAPVMLLPDDFKANSKIKVNYHLFNKENLPGHFKFKEYCPQVFRNLRERFGIEDQDYQASLVRSSPLKDSDSQDEHLQLTSYDRLLIIKQISSEEVEDMHNILSEYHQHIVKSHGVTLLPQFLGMYRVTVNNEDTYFIIMRNMFSHRLTIHTKYNLKGSLVSREASDKEKEKDLPTFKDMDFRNNMQRIYISDEEKEKLMEKLNRDIDFLVRLKLMDYSLLLAIHEMDRGEREEEEEDEESACEEEDEQENNMTMVASASLSTSPEGGTGSFPARRTVTPAEFDPRVDVYGIQSAAGAPQREVYFMGLINILTHYDTKKKAAHAAKTVKHGTGAEISTVHPEQYGKRFLEFIANIFTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>W5UMT9</accession>
    <name>W5UMT9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9 11">Tropomyosin alpha-1 chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tpma</name>
        <name evidence="11" type="synonym">LOC108271010</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the tropomyosin family.</text>
    </comment>
    <dbReference type="EMBL" id="JT419377">
        <property type="protein sequence ID" value="AHH43055.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017333646.1">
        <property type="nucleotide sequence ID" value="XM_017478157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UMT9"/>
    <dbReference type="SMR" id="W5UMT9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007271"/>
    <dbReference type="GeneID" id="108271010"/>
    <dbReference type="KEGG" id="ipu:108271010"/>
    <dbReference type="GeneTree" id="ENSGT01030000234542"/>
    <dbReference type="OrthoDB" id="128924at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000001">
        <property type="entry name" value="Tropomyosin alpha-1 chain isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000001">
        <property type="entry name" value="Tropomyosin alpha-1 chain isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000533">
        <property type="entry name" value="Tropomyosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19269">
        <property type="entry name" value="TROPOMYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00261">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00194">
        <property type="entry name" value="TROPOMYOSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00326">
        <property type="entry name" value="TROPOMYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AHH43055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017333646.1"/>
        </source>
    </evidence>
    <sequence length="284" mass="32619" checksum="A651092D9E87BC20" modified="2014-04-16" version="1">MDAIKKKMQMLKLDKENALDRAEQSEGDKKASEDRTKALEDDLVALQKKLKGTEDELDKYCEAVKDAQEKLEVADKKAADAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMEVQEIQLKEAKNIAEDADRKYEEVARKLVIVESELERTEERAELNEGKCSELEEELKTVTNLMKSLEAQAEKYSQKEDKYEEEIKVLTDKLKEAETRAEFSERSVAKLEKTVDDLEDELYAQKLKFKAISEELDHALNDMTSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W6EK53</accession>
    <name>W6EK53_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rhamnose binding lectin type IIIa</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="7">Rhamnose-binding lectin-like precursor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC108265029</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="5 7" key="2">
        <citation type="journal article" date="2014" name="Dev. Comp. Immunol." volume="44" first="320" last="331">
            <title>L-Rhamnose-binding lectins (RBLs) in channel catfish, Ictalurus punctatus: Characterization and expression profiling in mucosal tissues.</title>
            <authorList>
                <person name="Thongda W."/>
                <person name="Li C."/>
                <person name="Luo Y."/>
                <person name="Beck B.H."/>
                <person name="Peatman E."/>
            </authorList>
            <dbReference type="PubMed" id="24480296"/>
            <dbReference type="DOI" id="10.1016/j.dci.2014.01.018"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="KF725631">
        <property type="protein sequence ID" value="AHJ14697.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001316193.1">
        <property type="nucleotide sequence ID" value="NM_001329264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W6EK53"/>
    <dbReference type="GeneID" id="108265029"/>
    <dbReference type="KEGG" id="ipu:108265029"/>
    <dbReference type="GeneTree" id="ENSGT00940000154285"/>
    <dbReference type="OMA" id="EVAYACQ"/>
    <dbReference type="OrthoDB" id="1100386at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22836">
        <property type="entry name" value="Gal_Rha_Lectin_RBL_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.740:FF:000001">
        <property type="entry name" value="Adhesion G protein-coupled receptor L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.740">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000922">
        <property type="entry name" value="Lectin_gal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043159">
        <property type="entry name" value="Lectin_gal-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46780">
        <property type="entry name" value="PROTEIN EVA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02140">
        <property type="entry name" value="SUEL_Lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50228">
        <property type="entry name" value="SUEL_LECTIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1 5" id="KW-0430">Lectin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035982259" evidence="3 7">
        <location>
            <begin position="23"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="4">
        <location>
            <begin position="27"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="4">
        <location>
            <begin position="122"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AHJ14697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="NP_001316193.1"/>
        </source>
    </evidence>
    <sequence length="219" mass="24086" checksum="54A27AD353BFCDBF" modified="2014-04-16" version="1">MLLLKFTLLTLLIEAPDLLVSGENIITCYGDIQHLTCATGLIKVKSSVYGRTDRHTCSASRPASQVANTNCALRISTIADRCNGLKDCDLKTDLLGNPDPCFGTYKYYNTTYECIYGWYSVICEYGYSTLDCGDDSIQIINANYGRAESRTCSNGLPSVLTQNTNCYALNTFSTVSALCNGQRKCTVSADYTIFTDPCFGTLKYLTVSYKCISANRPDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PI11</accession>
    <name>A0A2D0PI11_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Intermediate filament family orphan 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">iffo2b</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017305497.1">
        <property type="nucleotide sequence ID" value="XM_017450008.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PI11"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009013"/>
    <dbReference type="GeneID" id="108254735"/>
    <dbReference type="KEGG" id="ipu:108254735"/>
    <dbReference type="CTD" id="100534822"/>
    <dbReference type="OrthoDB" id="9946830at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14516">
        <property type="entry name" value="1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14516:SF1">
        <property type="entry name" value="INTERMEDIATE FILAMENT FAMILY ORPHAN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="5">
        <location>
            <begin position="34"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="287"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="456"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="39"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="167"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="301"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="461"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="477"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017305497.1"/>
        </source>
    </evidence>
    <sequence length="493" mass="55723" checksum="6DC9C1CF7760F35A" modified="2017-12-20" version="1">MNSLLFGDMLSAGMLAALPGSGSGASALRDDLGSNIHVLKTLNLRFRRFLARVHELERRNKALESQLKLAAERVTYHPLFTRDVAVQTDSTEPRLPGTIWSYTHVRRRGERVETLHGPGVSWSHPDGVGVQIDTITPELRALYNFLAKVKRERDEYRTKWQIELSRREQMETMLETLQESAQESESIQEELRNKVERLKAELVVFKSLMSDGGRHPASRSRAAENMRRVPDLKQMSELDSKIQEKAMKVDMDICRRIDITAKLCDVAQQRNSEDIIFNVSPSRAPAGPVACRRKEKKALSDEETSEMDADPSTSEEEAAGPLNITDEMKRMLNQLRENFDIDDDCDSLTWEENEETLLLWEDFTNYNVPFISTTTNGADCEGGSQEADCSLGSLIDETETLFKTREEEYQATIGQIEMELATAKSDMNRHLHEYMEMCSMKRGLDVQMETCRRMIKGGRNSPSFSSVASSDSGNTDEIQDESDKDGDTEGAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PLA5</accession>
    <name>A0A2D0PLA5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sulfite oxidase</fullName>
            <ecNumber evidence="6">1.8.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19 20" type="primary">suox</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19 20" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19 20">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the oxidation of sulfite to sulfate, the terminal reaction in the oxidative degradation of sulfur-containing amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>sulfite + O2 + H2O = sulfate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:24600"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="EC" id="1.8.3.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:24601"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Energy metabolism; sulfur metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sulfur metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.8.3.1" evidence="6"/>
    <dbReference type="RefSeq" id="XP_017306201.1">
        <property type="nucleotide sequence ID" value="XM_017450712.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017306202.1">
        <property type="nucleotide sequence ID" value="XM_017450713.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PLA5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018841"/>
    <dbReference type="GeneID" id="108255064"/>
    <dbReference type="KEGG" id="ipu:108255064"/>
    <dbReference type="CTD" id="6821"/>
    <dbReference type="OMA" id="TWHVAEL"/>
    <dbReference type="OrthoDB" id="10051395at2759"/>
    <dbReference type="UniPathway" id="UPA00096"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008482">
        <property type="term" value="F:sulfite oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006790">
        <property type="term" value="P:sulfur compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02111">
        <property type="entry name" value="eukary_SO_Moco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.650:FF:000002">
        <property type="entry name" value="sulfite oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000007">
        <property type="entry name" value="Sulfite oxidase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.420.10:FF:000002">
        <property type="entry name" value="sulfite oxidase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.420.10">
        <property type="entry name" value="Oxidoreductase, molybdopterin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005066">
        <property type="entry name" value="MoCF_OxRdtse_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008335">
        <property type="entry name" value="Mopterin_OxRdtase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000572">
        <property type="entry name" value="OxRdtase_Mopterin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036374">
        <property type="entry name" value="OxRdtase_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19372:SF7">
        <property type="entry name" value="SULFITE OXIDASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19372">
        <property type="entry name" value="SULFITE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03404">
        <property type="entry name" value="Mo-co_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00174">
        <property type="entry name" value="Oxidored_molyb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00407">
        <property type="entry name" value="EUMOPTERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56524">
        <property type="entry name" value="Oxidoreductase molybdopterin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0349">Heme</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="17">
        <location>
            <begin position="94"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017306201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017306202.1"/>
        </source>
    </evidence>
    <sequence length="567" mass="62794" checksum="33E5DEA8A0674830" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PM83</accession>
    <name>A0A2D0PM83_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein LOC108255580</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC108255580</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017307142.1">
        <property type="nucleotide sequence ID" value="XM_017451653.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PM83"/>
    <dbReference type="GeneID" id="108255580"/>
    <dbReference type="KEGG" id="ipu:108255580"/>
    <dbReference type="OrthoDB" id="8876985at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012790753" evidence="2">
        <location>
            <begin position="22"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017307142.1"/>
        </source>
    </evidence>
    <sequence length="295" mass="32843" checksum="BFA13E093F70C46A" modified="2017-12-20" version="1">MWSYTLLFVLQIFSFAPTVSGDVSVTAKLHHPVTLTCNSRCSGVLKWIKFHKPGSVLARCDQTSCRSEEGFNISHDQYLKGNPHLTITAADYSARRLYTCLCDNKVVCNVRLLIEPLEMTHVIAPGEPIEVFLPHTDLVDVSFTASDAVQPSNLQICSVDKEKIQCSPDYKERASLLNTLQIKDGDVSDCGNYTVQDIVNDETLAIVTVHVREPSPDIKPCPDIKQQEGVPVWGIVLILMVLLLVSVLVVVSVMYVRLRREVHQLRENAENSRLALLQQNGNGNHPGEESNLKNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PPP1</accession>
    <name>A0A2D0PPP1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">39S ribosomal protein L41, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">mrpl41</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8 9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL41 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017307400.1">
        <property type="nucleotide sequence ID" value="XM_017451911.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017307401.1">
        <property type="nucleotide sequence ID" value="XM_017451912.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PPP1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009637"/>
    <dbReference type="GeneID" id="108255739"/>
    <dbReference type="KEGG" id="ipu:108255739"/>
    <dbReference type="CTD" id="64975"/>
    <dbReference type="GeneTree" id="ENSGT00390000013158"/>
    <dbReference type="OMA" id="DRMSAWT"/>
    <dbReference type="OrthoDB" id="408933at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019189">
        <property type="entry name" value="Ribosomal_mL41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21338:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21338">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN L41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09809">
        <property type="entry name" value="MRP-L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017307400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017307401.1"/>
        </source>
    </evidence>
    <sequence length="135" mass="15205" checksum="90063D8622201E18" modified="2017-12-20" version="1">MGLLSALTRGLVRGADRTAEFTSKRGPRTFYKSRGARPAGVLTSSRKFVPVREMIPEFVVPNLEGFRLKAYVSYRSPRGSEQPVTAESLFNQAVAPHIHRDIEAGTFDAQNLEKYGLEKTQEGKLFKLYPKNFVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PS89</accession>
    <name>A0A2D0PS89_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Selenocysteine insertion sequence-binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">LOC108256010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4 5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017307949.1">
        <property type="nucleotide sequence ID" value="XM_017452460.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047005746.1">
        <property type="nucleotide sequence ID" value="XM_047149790.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PS89"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022709"/>
    <dbReference type="GeneID" id="108256010"/>
    <dbReference type="KEGG" id="ipu:108256010"/>
    <dbReference type="OMA" id="MCREQGV"/>
    <dbReference type="OrthoDB" id="263617at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035368">
        <property type="term" value="F:selenocysteine insertion sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001514">
        <property type="term" value="P:selenocysteine incorporation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000004">
        <property type="entry name" value="selenocysteine insertion sequence-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040051">
        <property type="entry name" value="SECISBP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13284">
        <property type="entry name" value="GH01354P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13284:SF9">
        <property type="entry name" value="SELENOCYSTEINE INSERTION SEQUENCE-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="2">
        <location>
            <begin position="586"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="706"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="407"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="457"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="532"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017307949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_047005746.1"/>
        </source>
    </evidence>
    <sequence length="777" mass="87054" checksum="65EF873044126931" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PSA2</accession>
    <name>A0A2D0PSA2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein transport protein Sec61 subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">sec61g</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of SEC61 channel-forming translocon complex that mediates transport of signal peptide-containing precursor polypeptides across the endoplasmic reticulum (ER). Forms a ribosome receptor and a gated pore in the ER membrane, both functions required for cotranslational translocation of nascent polypeptides. The SEC61 channel is also involved in ER membrane insertion of transmembrane proteins: it mediates membrane insertion of the first few transmembrane segments of proteins, while insertion of subsequent transmembrane regions of multi-pass membrane proteins is mediated by the multi-pass translocon (MPT) complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">The SEC61 channel-forming translocon complex consists of channel-forming core components SEC61A1, SEC61B and SEC61G and different auxiliary components such as SEC62 and SEC63. The SEC61 channel associates with the multi-pass translocon (MPT) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017308892.1">
        <property type="nucleotide sequence ID" value="XM_017453403.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PSA2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019965"/>
    <dbReference type="GeneID" id="108256481"/>
    <dbReference type="KEGG" id="ipu:108256481"/>
    <dbReference type="CTD" id="23480"/>
    <dbReference type="OrthoDB" id="2401875at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.820:FF:000001">
        <property type="entry name" value="Transport protein Sec61 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.820">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023391">
        <property type="entry name" value="Prot_translocase_SecE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008158">
        <property type="entry name" value="Translocase_Sec61-g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00327">
        <property type="entry name" value="secE_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12309">
        <property type="entry name" value="SEC61 GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103456">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01067">
        <property type="entry name" value="SECE_SEC61G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017308892.1"/>
        </source>
    </evidence>
    <sequence length="68" mass="7711" checksum="14DC339A36408561" modified="2017-12-20" version="1">MDQVMQFVEPGRQFVKDSIRLVKRCTKPDRKEFQKIAMATAIGFAIMGFIGFFVKLIHIPINNIIVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PU96</accession>
    <name>A0A2D0PU96_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Dihydropyrimidinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">dpys</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="PTM">
        <text evidence="2">Carbamylation allows a single lysine to coordinate two divalent metal cations.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. Hydantoinase/dihydropyrimidinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017308996.1">
        <property type="nucleotide sequence ID" value="XM_017453507.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PU96"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033451"/>
    <dbReference type="GeneID" id="108256537"/>
    <dbReference type="KEGG" id="ipu:108256537"/>
    <dbReference type="CTD" id="1807"/>
    <dbReference type="OMA" id="SAETHHM"/>
    <dbReference type="OrthoDB" id="10258955at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004157">
        <property type="term" value="F:dihydropyrimidinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006208">
        <property type="term" value="P:pyrimidine nucleobase catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01314">
        <property type="entry name" value="D-HYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000076">
        <property type="entry name" value="Dihydropyrimidinase like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011778">
        <property type="entry name" value="Hydantoinase/dihydroPyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050378">
        <property type="entry name" value="Metallo-dep_Hydrolases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02033">
        <property type="entry name" value="D-hydantoinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647:SF50">
        <property type="entry name" value="DIHYDROPYRIMIDINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647">
        <property type="entry name" value="HYDRANTOINASE/DIHYDROPYRIMIDINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="3">
        <location>
            <begin position="55"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="2">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR611778-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017308996.1"/>
        </source>
    </evidence>
    <sequence length="499" mass="54645" checksum="3D971ADC8912B344" modified="2017-12-20" version="1">MSERGRILIKGGKVVNEECTVLADVFIEDGVIQEVGSELQLPESGTRVLDASGKLVLPGGIDTHTHMELEFMGTVAVDDFLSGTKAAVAGGTTMILDFVIPRKGTSLLEAYDRWRKIADAKVCCDYSLHVAVTWWDDSVKREMETLVQERGVNSFKTFMAYKDVFMLRDDELFSVFSHCKDIGALAQVHAENGDLIAEGTKKMLSLGITGPEGHELCRPEDVESEATQRAITIAHAANCPLYVVHVMSKSAAKVVAAARRNGRVVFGEPIAAGLGTDGTHYWHKDWAHAARFVMGPPLRPDPSTPGYLMDLLANDDLSVTGTDNCTFLICQKALGKDDFTKIPNGVNGVEDRMSVIWEKGVHSGKMDENRFVAVTSSSAAKIFNLYPQKGRIAKGSDADLVIWDPNATRVISAKTHHQAVDYNIFEGMECHGVPLVTISGGRVVYENGRVLAEPGTGRFIPRKPYSDFVYKRIRRRDEVGKPSAVVRTPYKGEVISTAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PWK8</accession>
    <name>A0A2D0PWK8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="18">C-type mannose receptor 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">mrc2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017310429.1">
        <property type="nucleotide sequence ID" value="XM_017454940.2"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000036098"/>
    <dbReference type="GeneID" id="108257292"/>
    <dbReference type="CTD" id="9902"/>
    <dbReference type="OMA" id="GFIWEHI"/>
    <dbReference type="OrthoDB" id="5858677at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="CDD" id="cd03590">
        <property type="entry name" value="CLECT_DC-SIGN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00062">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.10.10:FF:000001">
        <property type="entry name" value="Fibronectin 1a isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000020">
        <property type="entry name" value="Mannose receptor C type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000018">
        <property type="entry name" value="Mannose receptor, C type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.10.10">
        <property type="entry name" value="Fibronectin, type II, collagen-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033989">
        <property type="entry name" value="CD209-like_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000562">
        <property type="entry name" value="FN_type2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036943">
        <property type="entry name" value="FN_type2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24562">
        <property type="entry name" value="CysR_MRC2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00040">
        <property type="entry name" value="fn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00013">
        <property type="entry name" value="FNTYPEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00059">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00023">
        <property type="entry name" value="FN2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51092">
        <property type="entry name" value="FN2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0430">Lectin</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 18" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012722817" evidence="14">
        <location>
            <begin position="37"/>
            <end position="1475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1408"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-II" evidence="16">
        <location>
            <begin position="188"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="15">
        <location>
            <begin position="250"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="15">
        <location>
            <begin position="396"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="15">
        <location>
            <begin position="534"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="15">
        <location>
            <begin position="684"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="15">
        <location>
            <begin position="830"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="15">
        <location>
            <begin position="977"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="15">
        <location>
            <begin position="1129"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="15">
        <location>
            <begin position="1269"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="193"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="207"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017310429.1"/>
        </source>
    </evidence>
    <sequence length="1475" mass="167094" checksum="8CDFE7A5AECC6982" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PX79</accession>
    <name>A0A2D0PX79_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane protein 196</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC108257405</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017310637.1">
        <property type="nucleotide sequence ID" value="XM_017455148.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PX79"/>
    <dbReference type="GeneID" id="108257405"/>
    <dbReference type="KEGG" id="ipu:108257405"/>
    <dbReference type="OrthoDB" id="10016951at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037661">
        <property type="entry name" value="TMEM196"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28681">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 196"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28681:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 196"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013107478" description="Transmembrane protein 196" evidence="10">
        <location>
            <begin position="23"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="149"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="172"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017310637.1"/>
        </source>
    </evidence>
    <sequence length="200" mass="21607" checksum="69B9FB4D6A7C92E3" modified="2017-12-20" version="1">MCSSRKIVCSLLVLSVLQVGLGAASIALGAVAISRARHEHKTQQGHASPVWSGVCFLVCGLCGMLCARKRTGLVMILFSACCICGLIGGILNFQFVRALVKRPDTVRSLHMAAMSLACLGISSCTLSTWLTCRLASSEQQRMFLEREHSLHHSHEMSEKVDEERKRGEPSPGVSQCLSEVMDNSSNGIPQITYNSQSASP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PXE4</accession>
    <name>A0A2D0PXE4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Sodium-dependent multivitamin transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Solute carrier family 5 member 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26 27" type="primary">slc5a6a</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">SDA103</strain>
        </source>
    </reference>
    <reference evidence="26 27" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26 27">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Sodium-dependent multivitamin transporter that mediates the electrogenic transport of pantothenate, biotin, lipoate and iodide. Functions as a Na(+)-coupled substrate symporter where the stoichiometry of Na(+):substrate is 2:1, creating an electrochemical Na(+) gradient used as driving force for substrate uptake. Required for biotin and pantothenate uptake in the intestine across the brush border membrane. Plays a role in the maintenance of intestinal mucosa integrity, by providing the gut mucosa with biotin. Contributes to the luminal uptake of biotin and pantothenate into the brain across the blood-brain barrier.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(R)-lipoate(out) + 2 Na(+)(out) = (R)-lipoate(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:73379"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(R)-pantothenate(out) + 2 Na(+)(out) = (R)-pantothenate(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:73371"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>biotin(out) + 2 Na(+)(out) = biotin(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:73375"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>iodide(out) + 2 Na(+)(out) = iodide(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71207"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with PDZD11.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 22">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017311173.1">
        <property type="nucleotide sequence ID" value="XM_017455684.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053531573.1">
        <property type="nucleotide sequence ID" value="XM_053675598.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PXE4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030500"/>
    <dbReference type="GeneID" id="108257721"/>
    <dbReference type="KEGG" id="ipu:108257721"/>
    <dbReference type="CTD" id="563356"/>
    <dbReference type="GeneTree" id="ENSGT00940000155731"/>
    <dbReference type="OMA" id="GWWGMRR"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015075">
        <property type="term" value="F:monoatomic ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090482">
        <property type="term" value="F:vitamin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098660">
        <property type="term" value="P:inorganic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015887">
        <property type="term" value="P:pantothenate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1730.10:FF:000011">
        <property type="entry name" value="sodium-dependent multivitamin transporter isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018212">
        <property type="entry name" value="Na/solute_symporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051163">
        <property type="entry name" value="Sodium:Solute_Symporter_SSF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985">
        <property type="entry name" value="SODIUM-COUPLED MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42985:SF2">
        <property type="entry name" value="SODIUM-DEPENDENT MULTIVITAMIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00456">
        <property type="entry name" value="NA_SOLUT_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0092">Biotin</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="279"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="339"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="382"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="408"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="439"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="511"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="579"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="579"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="597"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00058802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00061728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00073170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00078601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_017311173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_053531573.1"/>
        </source>
    </evidence>
    <sequence length="624" mass="67855" checksum="AFDB6411DA879D12" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q0H3</accession>
    <name>A0A2D0Q0H3_ICTPU</name>
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        <submittedName>
            <fullName evidence="5">Kinesin-associated protein 3a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">kifap3a</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017311794.1">
        <property type="nucleotide sequence ID" value="XM_017456305.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q0H3"/>
    <dbReference type="GeneID" id="108258047"/>
    <dbReference type="KEGG" id="ipu:108258047"/>
    <dbReference type="CTD" id="447906"/>
    <dbReference type="OrthoDB" id="10265679at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016939">
        <property type="term" value="C:kinesin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008658">
        <property type="entry name" value="KAP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15605:SF2">
        <property type="entry name" value="KINESIN-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15605">
        <property type="entry name" value="KINESIN-ASSOCIATED PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05804">
        <property type="entry name" value="KAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM01297">
        <property type="entry name" value="KAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="189"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="1">
        <location>
            <begin position="602"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="225"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017311794.1"/>
        </source>
    </evidence>
    <sequence length="805" mass="92098" checksum="DF814BDFCAF42220" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q2Z3</accession>
    <name>A0A2D0Q2Z3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Zinc finger protein 410</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">znf410</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Transcription factor that binds to the sequence motif 5'-CATCCCATAATA-3', and is specifically required to silence expression of fetal hemoglobin in adult erythroid cells. Prevents expression of fetal hemoglobin genes HBG1 and HBG2 through CHD4: acts as a direct transcriptional activator of CHD4, a central component of the NuRD complex that represses transcription of fetal hemoglobin genes HBG1 and HBG2 in erythroid cells. May also activate transcription of matrix-remodeling genes such as MMP1 during fibroblast senescence. May activate transcription of the gap junction gene GJC1, perhaps in response to increasing glucose. However, recent studies suggest that ZNF410 is dedicated to regulate expression of a single gene: CHD4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with CDKN2A/p14ARF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017311725.1">
        <property type="nucleotide sequence ID" value="XM_017456236.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q2Z3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029929"/>
    <dbReference type="GeneID" id="108258012"/>
    <dbReference type="KEGG" id="ipu:108258012"/>
    <dbReference type="CTD" id="57862"/>
    <dbReference type="OMA" id="XNDRSFI"/>
    <dbReference type="OrthoDB" id="5977959at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000071">
        <property type="entry name" value="Putative zinc finger protein 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000221">
        <property type="entry name" value="Zinc finger protein 410"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000462">
        <property type="entry name" value="Zinc finger protein 410"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000441">
        <property type="entry name" value="zinc finger protein 410 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF23">
        <property type="entry name" value="ZINC FINGER PROTEIN 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="205"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="235"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="265"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="295"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="325"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00055956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00064830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00067821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017311725.1"/>
        </source>
    </evidence>
    <sequence length="432" mass="47359" checksum="5C0FDA4C35AFC368" modified="2017-12-20" version="1">MLSDELDSKPELLVEFVQNASIPLGQGLEESDSKTQRLPLLPSSDNSICSQLDLPESALSHAASPSLSEFGPERSPLVVQLQSHSPTPTPTAILQDLQQPDSTSYVLLNLAKGLAASTEPLVFVQDDVDEAEEEISAGECTDGSAPWYLRVQELAHDSLIAATRAELAKEARASSNSDHILSCQSEGPKKETPPRNSRASAEKILRCPYENCQRTFTYPAHLKYHLKTHRNDRTFRCGAEGCGKSFYVLQRLQVHMRTHNGEKPFICNEKNCGKKFTTAGNLKNHKRTHTGEKPFLCDVDNCGRSFAEYSSLRKHMLVHSGEKPHQCSICGKMFSQSGSRNVHMRKRHGEDTLMSESRETGEALTHSSLLEADGAATDSMVTMTTVEPVNLHHAMLRAQGPAEPIVVLSPSHDLVNITAGAHGYGEDVVALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QCK2</accession>
    <name>A0A2D0QCK2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Coiled-coil domain-containing protein 43</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ccdc43</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC43 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017315441.1">
        <property type="nucleotide sequence ID" value="XM_017459952.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QCK2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018460"/>
    <dbReference type="GeneID" id="108260032"/>
    <dbReference type="KEGG" id="ipu:108260032"/>
    <dbReference type="CTD" id="124808"/>
    <dbReference type="OMA" id="KFLFRNT"/>
    <dbReference type="OrthoDB" id="2187466at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037666">
        <property type="entry name" value="CCDC43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058771">
        <property type="entry name" value="PWI_CCDC43"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31684">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31684:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26091">
        <property type="entry name" value="PWI_CCDC43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCDC43 PWI-like" evidence="5">
        <location>
            <begin position="8"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="128"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="131"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="171"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="203"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF26091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017315441.1"/>
        </source>
    </evidence>
    <sequence length="215" mass="24575" checksum="68203F24E26C8F94" modified="2017-12-20" version="1">MAAPDFSAGEFEKWLNELLDGFGADREVYGVYILGVLQEEETDEEKSDALNGILSAFVDEDTLEEVCQQILKQWTDCCSRTAAASNQPEDEVQAIASLIEKQAQIVVRQKAVSEEAKQRKEALLAQYANVTDDEDEGEDEEDVAGALGIPSDKSLFKNTNVEEVMSRRRQQREQAKEDAQKKKEQDKMQREKDKLTKQERKDREKKRTQKGERKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QH81</accession>
    <name>A0A2D0QH81_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Histone H2B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC108261488</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="12">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017317848.1">
        <property type="nucleotide sequence ID" value="XM_017462359.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QH81"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012364"/>
    <dbReference type="GeneID" id="108261488"/>
    <dbReference type="KEGG" id="ipu:108261488"/>
    <dbReference type="OMA" id="FCPFAIR"/>
    <dbReference type="OrthoDB" id="10036053at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000003">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 12" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11 12" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="14">
        <location>
            <begin position="23"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017317848.1"/>
        </source>
    </evidence>
    <sequence length="124" mass="13622" checksum="DE0B307C4614078D" modified="2017-12-20" version="1">MPDPAKTAPKKGLKKAVTKTAGKGGKKRRKSRKESYAIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGESSRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QLQ5</accession>
    <name>A0A2D0QLQ5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed RNA polymerases I and III subunit RPAC1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">polr1c</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and short non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs, respectively. POLR1C/RPAC1 is part of the polymerase core and may function as a clamp element that moves to open and close the cleft.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017319219.1">
        <property type="nucleotide sequence ID" value="XM_017463730.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QLQ5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000036536"/>
    <dbReference type="GeneID" id="108263181"/>
    <dbReference type="KEGG" id="ipu:108263181"/>
    <dbReference type="CTD" id="9533"/>
    <dbReference type="OMA" id="KKKCRAF"/>
    <dbReference type="OrthoDB" id="270173at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07032">
        <property type="entry name" value="RNAP_I_II_AC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.120.12:FF:000003">
        <property type="entry name" value="Dna-directed rna polymerases i and iii subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.10:FF:000005">
        <property type="entry name" value="Dna-directed rna polymerases i and iii subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.10:FF:000009">
        <property type="entry name" value="RNA polymerase I and III subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00320">
        <property type="entry name" value="RNApol_arch_Rpo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001514">
        <property type="entry name" value="DNA-dir_RNA_pol_30-40kDasu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022842">
        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033901">
        <property type="entry name" value="RNAPI/III_AC40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050518">
        <property type="entry name" value="Rpo3/RPB3_RNA_Pol_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001988">
        <property type="entry name" value="PRK00783.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800:SF13">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00446">
        <property type="entry name" value="RNA_POL_D_30KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 13" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RpoA/D/Rpb3-type" evidence="11">
        <location>
            <begin position="61"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017319219.1"/>
        </source>
    </evidence>
    <sequence length="348" mass="39186" checksum="6E84AB4A904FAB45" modified="2017-12-20" version="1">MAVPMSNVDEIRDRVILGEFGVKNVHSTNFPGNYPGYDDTWDLGKFKKNFRLDVVHMDENTLEFDMVGIDAAIANAFRRILLAEVPTMAVEKVFIYNNTSIIQDEILAHRLGLVPIKADPRLFEYRNTGDEEGTEIDTIHLQLKIKCTRNPRASKDASDPKELYLNHMVYSRDMKWVPIGNQADVFADANIGPVHGDILLAQLRPGQELDIVMHCVKGIGKDHAKFSPVATASYRLLPEITLLKTIEGEKAERLKRCFSPGVIELEDVDGKQVAKVVNSRLDTCSREVLRHDDLKSLVKLGRVRDHFLFSVESTGILPPDVLFSEAVNVLIAKCQKFLTELDSVNEME</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QNR6</accession>
    <name>A0A2D0QNR6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphatase and actin regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">phactr2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">Binds PPP1CA and actin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the phosphatase and actin regulator family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017320042.1">
        <property type="nucleotide sequence ID" value="XM_017464553.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QNR6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022270"/>
    <dbReference type="GeneID" id="108263585"/>
    <dbReference type="KEGG" id="ipu:108263585"/>
    <dbReference type="CTD" id="9749"/>
    <dbReference type="OrthoDB" id="5563016at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004018">
        <property type="entry name" value="RPEL_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12751:SF5">
        <property type="entry name" value="PHOSPHATASE AND ACTIN REGULATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12751">
        <property type="entry name" value="PHOSPHATASE AND ACTIN REGULATOR PHACTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02755">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00707">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51073">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RPEL" evidence="4">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="repeat" description="RPEL" evidence="4">
        <location>
            <begin position="476"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="repeat" description="RPEL" evidence="4">
        <location>
            <begin position="514"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="repeat" description="RPEL" evidence="4">
        <location>
            <begin position="552"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="133"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="523"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="189"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="205"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="314"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="359"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="379"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="397"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="453"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="487"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="525"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU301113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017320042.1"/>
        </source>
    </evidence>
    <sequence length="633" mass="69818" checksum="9C4F1A6F8FF2BAF5" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QTG7</accession>
    <name>A0A2D0QTG7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Cytochrome b-245 light chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 15">Cytochrome b(558) alpha chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10 15">Cytochrome b558 subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13 15">Neutrophil cytochrome b 22 kDa polypeptide</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12 15">Superoxide-generating NADPH oxidase light chain subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8 15">p22 phagocyte B-cytochrome</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 15">p22-phox</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC108264585</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Subunit of NADPH oxidase complexes that is required for the NADPH oxidase activity that generates, in various cell types, superoxide from molecular oxygen utilizing NADPH as an electron donor. Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction. Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane. This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox. Aassociates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the phagocyte NADPH oxidase core complex/cytochrome b558 complex, composed of CYBB (heavy chain (beta)) and CYBA (light chain (alpha)). Component of the phagocyte NADPH oxidase complex composed of an obligatory core heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic regulatory subunits NCF1/p47-phox, NCF2/p67-phox, NCF4/p40-phox and the small GTPase RAC1 or RAC2. Interacts with NCF1 (via SH3 domain). Interacts with SH3PXD2A. Interacts with DUOX1, DUOX2 and TPO. Interacts with NOX4; this interaction mediates superoxide generation. Interacts with calprotectin (S100A8/9). Interacts with GBP7. Interacts with NOXO1. Forms a heterodimer with NOX3 and is essential for activity and cell membrane localization of NOX3. Interacts with NOX1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the p22phox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017321723.1">
        <property type="nucleotide sequence ID" value="XM_017466234.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QTG7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000010322"/>
    <dbReference type="GeneID" id="108264585"/>
    <dbReference type="KEGG" id="ipu:108264585"/>
    <dbReference type="OMA" id="ARTGQYC"/>
    <dbReference type="OrthoDB" id="2445232at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043020">
        <property type="term" value="C:NADPH oxidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007732">
        <property type="entry name" value="Cyt_b558_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15168">
        <property type="entry name" value="CYTOCHROME B-245 LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15168:SF0">
        <property type="entry name" value="CYTOCHROME B-245 LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05038">
        <property type="entry name" value="Cytochrom_B558a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019635">
        <property type="entry name" value="Cytochr_b558a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0249">Electron transport</keyword>
    <keyword evidence="15" id="KW-0349">Heme</keyword>
    <keyword evidence="15" id="KW-0408">Iron</keyword>
    <keyword evidence="7 15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0521">NADP</keyword>
    <keyword evidence="15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="140"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR019635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017321723.1"/>
        </source>
    </evidence>
    <sequence length="186" mass="20238" checksum="64378E32C0782E51" modified="2017-12-20" version="1">MGKIEWAMWANEQALAAGLIYLTGGIVGVAGQFRDWQFAAFGIAAGVFVCVLEYPRSRRAKGTSVERTGQYCFTMCVNAFGPLTRNYYVRAILHAALSVPGGFMLATLLGCVCLGISSLIYLSAAIHGEHWEPILPKAEPTKHVAPSIKQPPQNPPPRPPAEIRRKRTDDPEAASYANPIPVTDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QW23</accession>
    <name>A0A2D0QW23_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Rho guanine nucleotide exchange factor 16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">arhgef16</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017322622.1">
        <property type="nucleotide sequence ID" value="XM_017467133.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QW23"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032057"/>
    <dbReference type="GeneID" id="108265048"/>
    <dbReference type="KEGG" id="ipu:108265048"/>
    <dbReference type="CTD" id="27237"/>
    <dbReference type="OMA" id="WALNAMS"/>
    <dbReference type="OrthoDB" id="27593at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01221">
        <property type="entry name" value="PH_ephexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11938">
        <property type="entry name" value="SH3_ARHGEF16_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000007">
        <property type="entry name" value="rho guanine nucleotide exchange factor 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035797">
        <property type="entry name" value="ARHGEF16/ARHGEF26_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047271">
        <property type="entry name" value="Ephexin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047270">
        <property type="entry name" value="PH_ephexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12845">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12845:SF3">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="DH" evidence="9">
        <location>
            <begin position="256"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="473"/>
            <end position="579"/>
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    </feature>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="587"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="151"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="169"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017322622.1"/>
        </source>
    </evidence>
    <sequence length="667" mass="76942" checksum="596EE7055B60FD98" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QWG7</accession>
    <name>A0A2D0QWG7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Mitogen-activated protein kinase kinase kinase</fullName>
            <ecNumber evidence="3 13">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">map3k10</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="13">Homodimerization via the leucine zipper domains is required for autophosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="3 13"/>
    <dbReference type="RefSeq" id="XP_017322091.3">
        <property type="nucleotide sequence ID" value="XM_017466602.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QWG7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000017837"/>
    <dbReference type="GeneID" id="108264755"/>
    <dbReference type="KEGG" id="ipu:108264755"/>
    <dbReference type="CTD" id="4294"/>
    <dbReference type="OrthoDB" id="339325at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004706">
        <property type="term" value="F:JUN kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12059">
        <property type="entry name" value="SH3_MLK1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000076">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000079">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000085">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035779">
        <property type="entry name" value="MLK1-3_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016231">
        <property type="entry name" value="MLK1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF39">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000556">
        <property type="entry name" value="MAPKKK9_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 13 23" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 13 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 16" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SH3" evidence="20">
        <location>
            <begin position="77"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="163"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="550"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="605"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="747"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="874"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="442"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="56"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="621"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="747"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="772"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="982"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="287"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="169"/>
            <end position="177"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15 17">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR000556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000556-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000556-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017322091.3"/>
        </source>
    </evidence>
    <sequence length="1050" mass="114928" checksum="C96A1C6B554C0C5E" modified="2023-06-28" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QZ06</accession>
    <name>A0A2D0QZ06_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Splicing factor 3A subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">sf3a1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3A1 is part of the SF3A subcomplex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex. Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the 17S U2 SnRNP complex, a ribonucleoprotein complex that contains small nuclear RNA (snRNA) U2 and a number of specific proteins. Part of the SF3A subcomplex of the 17S U2 SnRNP complex which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62 and SF3A1/SAP114. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the mature 17S U2 small nuclear ribonucleoprotein complex (17S U2 snRNP). SF3A1 functions as a scaffold that interacts directly with both SF3A2 and SF3A3. Identified in the spliceosome 'E' complex, a precursor of the spliceosome 'A' complex. Identified in the spliceosome 'A' and 'B' complexes. Identified in the spliceosome 'C' complex. Interacts with P2RX6; resulting in a reduction of the splicing activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017468060.3"/>
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    <dbReference type="KEGG" id="ipu:108265570"/>
    <dbReference type="CTD" id="10291"/>
    <dbReference type="OrthoDB" id="447637at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01800">
        <property type="entry name" value="Ubl_SF3a120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.790:FF:000002">
        <property type="entry name" value="Splicing factor 3A subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000091">
        <property type="entry name" value="Splicing factor 3A subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.790:FF:000001">
        <property type="entry name" value="Splicing factor 3a, subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.790">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045146">
        <property type="entry name" value="SF3A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022030">
        <property type="entry name" value="SF3A1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035563">
        <property type="entry name" value="SF3As1_ubi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000061">
        <property type="entry name" value="Surp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035967">
        <property type="entry name" value="SWAP/Surp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15316">
        <property type="entry name" value="SPLICEOSOME ASSOCIATED PROTEIN 114/SWAP SPLICING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15316:SF1">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12230">
        <property type="entry name" value="PRP21_like_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01805">
        <property type="entry name" value="Surp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00648">
        <property type="entry name" value="SWAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109905">
        <property type="entry name" value="Surp module (SWAP domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50128">
        <property type="entry name" value="SURP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="SURP motif" evidence="16">
        <location>
            <begin position="41"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="SURP motif" evidence="16">
        <location>
            <begin position="156"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="15">
        <location>
            <begin position="712"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="307"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="528"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="661"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="310"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="335"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="345"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="360"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="379"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="547"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="661"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060058"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00074916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017323549.1"/>
        </source>
    </evidence>
    <sequence length="798" mass="90519" checksum="AA25160B18EFFAFE" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QZH3</accession>
    <name>A0A2D0QZH3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Collagen, type II, alpha 1a isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">col2a1a</name>
    </gene>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="13">SDA103</strain>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
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            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ARTD/PARP family.</text>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
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        <property type="term" value="F:NAD+-protein mono-ADP-ribosyltransferase activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:transcription corepressor activity"/>
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        <property type="term" value="P:negative regulation of gene expression"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:protein poly-ADP-ribosylation"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Macro_Af1521_BAL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Macro_BAL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
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    </dbReference>
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        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Macro_dom-like"/>
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        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
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        <property type="entry name" value="RRM_PARP14_2"/>
    </dbReference>
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        <property type="entry name" value="WWE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037197">
        <property type="entry name" value="WWE_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14453">
        <property type="entry name" value="PARP/ZINC FINGER CCCH TYPE DOMAIN CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR14453:SF89">
        <property type="entry name" value="PROTEIN MONO-ADP-RIBOSYLTRANSFERASE PARP14"/>
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        <property type="entry name" value="KH_PARP14_1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="KH_PARP14_8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Macro"/>
        <property type="match status" value="3"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF23245">
        <property type="entry name" value="RRM_PARP14_2"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF23085">
        <property type="entry name" value="RRM_PARP14_3"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="A1pp"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117839">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50918">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 7" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Macro" evidence="11">
        <location>
            <begin position="766"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="domain" description="Macro" evidence="11">
        <location>
            <begin position="984"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="domain" description="Macro" evidence="11">
        <location>
            <begin position="1191"/>
            <end position="1365"/>
        </location>
    </feature>
    <feature type="domain" description="WWE" evidence="9">
        <location>
            <begin position="1519"/>
            <end position="1595"/>
        </location>
    </feature>
    <feature type="domain" description="PARP catalytic" evidence="10">
        <location>
            <begin position="1605"/>
            <end position="1799"/>
        </location>
    </feature>
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        <location>
            <begin position="90"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="956"/>
            <end position="989"/>
        </location>
    </feature>
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        <location>
            <begin position="92"/>
            <end position="108"/>
        </location>
    </feature>
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        <location>
            <begin position="116"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="978"/>
            <end position="988"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362114"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017325149.1"/>
        </source>
    </evidence>
    <sequence length="1799" mass="200884" checksum="26184BFCBDC4F9ED" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R737</accession>
    <name>A0A2D0R737_ICTPU</name>
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        <recommendedName>
            <fullName evidence="4">Fibroblast growth factor</fullName>
            <shortName evidence="4">FGF</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">fgf14</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017326403.1">
        <property type="nucleotide sequence ID" value="XM_017470914.3"/>
    </dbReference>
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    <dbReference type="KEGG" id="ipu:108267035"/>
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    <dbReference type="OMA" id="KRINSPH"/>
    <dbReference type="OrthoDB" id="6158176at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000001">
        <property type="entry name" value="Fibroblast growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00263">
        <property type="entry name" value="HBGFFGF"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00442">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00247">
        <property type="entry name" value="HBGF_FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="214"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="241"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU049442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017326403.1"/>
        </source>
    </evidence>
    <sequence length="250" mass="27954" checksum="62E50004F4E87605" modified="2017-12-20" version="1">MAAAIASGLIRQKRQAREQHLDRPSTNRRRKSPNKSKGLCNGNLVDIFAKVRIFGLRKRRLRRQDPQLKGIVTRLYCRQGYYLQMNADGSLDGTKDDSSNSSLFNLIPVGLRVVAIQSVKTGLYIAMNGEGHLYTSELFTAECKFKESVFENYYVIYSSMLYRQQESGRAWFLGLNKEGQAMKGNRVKKTKPAAHFLPKPLEVAMYREPSLHDVGETLPKTAAPPAKSTSEPVVMNGGKPVSKEASKPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R812</accession>
    <name>A0A2D0R812_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Neuropilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">nrp2b</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">SDA103</strain>
        </source>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="27">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the neuropilin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017325970.2">
        <property type="nucleotide sequence ID" value="XM_017470481.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R812"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000016339"/>
    <dbReference type="GeneID" id="108266767"/>
    <dbReference type="KEGG" id="ipu:108266767"/>
    <dbReference type="CTD" id="405902"/>
    <dbReference type="OrthoDB" id="6155811at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017154">
        <property type="term" value="F:semaphorin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005021">
        <property type="term" value="F:vascular endothelial growth factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000002">
        <property type="entry name" value="Coagulation factor VIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000013">
        <property type="entry name" value="Neuropilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000003">
        <property type="entry name" value="Neuropilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000010">
        <property type="entry name" value="Neuropilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014648">
        <property type="entry name" value="Neuropilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022579">
        <property type="entry name" value="Neuropilin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050633">
        <property type="entry name" value="Neuropilin_MCO_CoagFactor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806">
        <property type="entry name" value="F5/8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806:SF2">
        <property type="entry name" value="NEUROPILIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11980">
        <property type="entry name" value="DUF3481"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036960">
        <property type="entry name" value="Neuropilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00020">
        <property type="entry name" value="MAMDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01285">
        <property type="entry name" value="FA58C_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01286">
        <property type="entry name" value="FA58C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00740">
        <property type="entry name" value="MAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17 18" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 17" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9 17" id="KW-0221">Differentiation</keyword>
    <keyword evidence="14 19 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="13 17 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 17" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="15 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 22" id="KW-0732">Signal</keyword>
    <keyword evidence="5 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039890880" description="Neuropilin" evidence="22">
        <location>
            <begin position="23"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="855"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="23">
        <location>
            <begin position="27"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="23">
        <location>
            <begin position="147"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="24">
        <location>
            <begin position="275"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="24">
        <location>
            <begin position="432"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="25">
        <location>
            <begin position="629"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="19 20">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="147"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="275"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="432"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR036960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR036960-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR036960-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_017325970.2"/>
        </source>
    </evidence>
    <sequence length="920" mass="102797" checksum="99813D0215DB8431" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R8I7</accession>
    <name>A0A2D0R8I7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cystatin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC108267382</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SDA103</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017326903.3">
        <property type="nucleotide sequence ID" value="XM_017471414.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R8I7"/>
    <dbReference type="GeneID" id="108267382"/>
    <dbReference type="KEGG" id="ipu:108267382"/>
    <dbReference type="OrthoDB" id="8894922at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_017326903.3"/>
        </source>
    </evidence>
    <sequence length="257" mass="28990" checksum="12528EB386AEE25A" modified="2023-06-28" version="3">MVDPKRKELGQKNYQKCNEKSSWRIAPYEQGQCILEKQIIQNCFGKPAWRVQQVPLTPIDGTQLRHPDQPTIGKVQQGSYKVRHDLRHTHSSVFLHNMDGALKALLLAALILLVSAQTSDNYRNLNGLVRKHVDKALKKANQDFGAGYHIAFHSLITTPMTRESILNVNVLLKVTTCKKTSNDAYEHRDECNEQKEKTPVIDCLVCKANDGNELVDCAKKIDVINKKNTKIRGKCIDHFTGGSDILTSKLSEDDEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RCG2</accession>
    <name>A0A2D0RCG2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Sal-like protein 3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">si:ch211-212k18.5</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the sal C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017327821.1">
        <property type="nucleotide sequence ID" value="XM_017472332.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RCG2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003651"/>
    <dbReference type="GeneID" id="108267861"/>
    <dbReference type="KEGG" id="ipu:108267861"/>
    <dbReference type="OMA" id="CVPPHRD"/>
    <dbReference type="OrthoDB" id="8922241at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000556">
        <property type="entry name" value="sal-like protein 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000025">
        <property type="entry name" value="Spalt-like transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001465">
        <property type="entry name" value="Zinc finger protein 560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051565">
        <property type="entry name" value="Sal_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23233">
        <property type="entry name" value="SAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23233:SF85">
        <property type="entry name" value="SAL-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="374"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="402"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="720"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="748"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="781"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="1109"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="230"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="442"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="533"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="592"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="851"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="960"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1121"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="93"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="147"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="533"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="564"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="620"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="660"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="851"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="867"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="960"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1060"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1133"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1158"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1170"/>
            <end position="1181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017327821.1"/>
        </source>
    </evidence>
    <sequence length="1205" mass="127876" checksum="BB91EFE2D1D0E698" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0REM9</accession>
    <name>A0A2D0REM9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Polyamine-modulated factor 1-binding protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC108268477</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017328978.1">
        <property type="nucleotide sequence ID" value="XM_017473489.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0REM9"/>
    <dbReference type="GeneID" id="108268477"/>
    <dbReference type="KEGG" id="ipu:108268477"/>
    <dbReference type="OrthoDB" id="10375536at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4090">
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="127"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="231"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="284"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017328978.1"/>
        </source>
    </evidence>
    <sequence length="386" mass="44964" checksum="D29B5CFCD9114E82" modified="2017-12-20" version="1">MSKSVKLRSRVGKPLKAQLSTKDETSNLKDQVKILQGSLEKLEGQVHKTRRMFKKKLKSFMAEDKKIVKIVVASTAQQEYENSIMKTQLKTLQGSVEKLEGLHKTDHIEEKKLKEQEQKLQKSLILCYDYKETRDTLEQENSDLKDEVKTLQASVETLEGQLHETHLMHERKLKSSLAESEERIKIALESNAQLERKNSILMEQLKTLQGPEEKLERRLCETHETRVKLLKDTLEQDNSDLKDEVKTLHCSLDRLEEELRDTNRMLEKKLKELQKSQVLWYQSEETKDTPEQENSDLKDEVKTPQASVETVEGQVHESQRFNEEILNERDPEQQKYITLLSKYEAIKSEAQENTLNAIAEIENKKFALMKQLGILRYSLEDCATLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RJK7</accession>
    <name>A0A2D0RJK7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">5-hydroxytryptamine receptor 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Serotonin receptor 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">htr4</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">G-protein coupled receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone and a mitogen. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. HTR4 is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts (via C-terminus 330-346 AA) with GRK5; this interaction is promoted by 5-HT (serotonin).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017330714.1">
        <property type="nucleotide sequence ID" value="XM_017475225.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RJK7"/>
    <dbReference type="GeneID" id="108269380"/>
    <dbReference type="KEGG" id="ipu:108269380"/>
    <dbReference type="CTD" id="3360"/>
    <dbReference type="OrthoDB" id="5859976at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004938">
        <property type="term" value="F:alpha2-adrenergic receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071881">
        <property type="term" value="P:adenylate cyclase-inhibiting adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032098">
        <property type="term" value="P:regulation of appetite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003008">
        <property type="term" value="P:system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15056">
        <property type="entry name" value="7tmA_5-HT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000046">
        <property type="entry name" value="5-hydroxytryptamine receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001520">
        <property type="entry name" value="5HT4_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF169">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01059">
        <property type="entry name" value="5HT4RECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8 17" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 17 21" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 17" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 17 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="330"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="19">
        <location>
            <begin position="76"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017330714.1"/>
        </source>
    </evidence>
    <sequence length="424" mass="47588" checksum="94526DB693C31546" modified="2017-12-20" version="1">MRGCAFPHDGLEPCHGFDPGSPRENETLFFYGYMNLSSSGQMPVMEEVDTSESNGMAKRVALISFLSLVMLMSVLGNLLVMVAVCKDRQLRKIKTNYFIVSLAFADLLVSVLVMPFGAIELIHQNWIYGETFCLVRTSLDVLLTTASILHLCCISLDRYYAICCQPLVYRNKMTPLRVALMIGGCWTIPTVISFLPIMQGWNSIGINDLIEARKFSGNSTVCVFMVNKPYALTCSVVAFYIPLGLMVLAYQRIYVTAREHARQIGMLQRAGGAASGLESAEHQRNHRMRTETKAAKTLCIIMGCFCLCWAPFFITNVVDPFIDYTVPEQLWAACLWLGYINSMLNPILYAFLNKSFRRAFLIILCCGHQRYRRPSILAARTPCTATQINGSTHVLKYSVLHNGNNSEQEKLSVLNDTESQESCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RL67</accession>
    <name>A0A2D0RL67_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="18">E3 SUMO-protein ligase EGR2a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">egr2a</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the EGR C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017330891.1">
        <property type="nucleotide sequence ID" value="XM_017475402.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RL67"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035608"/>
    <dbReference type="GeneID" id="108269483"/>
    <dbReference type="KEGG" id="ipu:108269483"/>
    <dbReference type="CTD" id="368241"/>
    <dbReference type="OMA" id="MRCLPSH"/>
    <dbReference type="OrthoDB" id="10018191at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000092">
        <property type="entry name" value="Early growth response protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021849">
        <property type="entry name" value="EGR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF60">
        <property type="entry name" value="STRIPE, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11928">
        <property type="entry name" value="DUF3446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="18" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="11 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="285"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="315"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="343"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="250"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="356"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="360"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="377"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017330891.1"/>
        </source>
    </evidence>
    <sequence length="387" mass="42421" checksum="988216083B1FA364" modified="2017-12-20" version="1">MTANSVDKVSESLTDFIRAIATDVCPMDEIPPDIPSGFPHVDVRSEASGGSLGAESFSKEKRIADFIDPNSTQSSPLAYTGKISIGAQCAHGSWSPDSLINFVCAEVQNATPGLSPFPTASPGPTDLSGASACAKSHEKDMEHMYTSPPPYTCSADGYQDPSTYLSTSTCPLTYPPASYTSPKSSIDGALFSIIPDYGGFYQSSGQRDAPVFQDRKPFSCSLDSVRVPPPLTPLNMIRNFTLSCPVDGLRPPTDSNPQSVPLRPILRPRKYPTRPCKTPVHERPYPCPAEGCDRRFSRSDELTRHVRIHTGHKPFQCRICMRSFSRSDHLTTHIRTHTGEKPFSCDFCGRKFARSDERRRHTKIHQRQREKRASAPPAQTSGAQGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RMG2</accession>
    <name>A0A2D0RMG2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ttbk1b</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017331336.1">
        <property type="nucleotide sequence ID" value="XM_017475847.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RMG2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027281"/>
    <dbReference type="GeneID" id="108269810"/>
    <dbReference type="KEGG" id="ipu:108269810"/>
    <dbReference type="CTD" id="100000353"/>
    <dbReference type="OrthoDB" id="5979581at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000167">
        <property type="entry name" value="Tau tubulin kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000358">
        <property type="entry name" value="Tau tubulin kinase 2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
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    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="32"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="405"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="526"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="626"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
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            <begin position="744"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="313"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="448"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="543"/>
            <end position="562"/>
        </location>
    </feature>
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            <end position="757"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
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        </location>
    </feature>
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        <location>
            <begin position="802"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="812"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="877"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="942"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="980"/>
            <end position="993"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1013"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1027"/>
            <end position="1038"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1039"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
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        </location>
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        <location>
            <begin position="1107"/>
            <end position="1126"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1164"/>
            <end position="1176"/>
        </location>
    </feature>
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        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017331336.1"/>
        </source>
    </evidence>
    <sequence length="1176" mass="131204" checksum="7A5E65F190A57ED6" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RMZ4</accession>
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        <submittedName>
            <fullName evidence="9">Eva-1 homolog Ba isoform X2</fullName>
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        <name evidence="9" type="primary">eva1ba</name>
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    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
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        <text evidence="2">Belongs to the EVA1 family.</text>
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    </dbReference>
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        <property type="component" value="Chromosome 1"/>
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        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="EVA1_A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039500">
        <property type="entry name" value="EVA1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48422:SF2">
        <property type="entry name" value="PROTEIN EVA-1 HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48422">
        <property type="entry name" value="PROTEIN EVA-1 HOMOLOG B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14851">
        <property type="entry name" value="FAM176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        <location>
            <begin position="23"/>
            <end position="143"/>
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        <location>
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        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017331310.1"/>
        </source>
    </evidence>
    <sequence length="145" mass="16635" checksum="23DD88C179776609" modified="2017-12-20" version="1">MDAKRKEMDLLSNSIAAYAHIKVIRISCKPRTTTAPLTPERKQLKDFCEDDEDEDSDDEEEEEEEENEDKVGDVEATITHCSTEIPMGNHHSTLDGNVNVFTSAEELERAQRLEERERIIREIWRNGQPDILGTGTSTIGRVHYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RQR6</accession>
    <name>A0A2D0RQR6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Protein CCSMST1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC108270539</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
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            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the UQCC4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017332839.1">
        <property type="nucleotide sequence ID" value="XM_017477350.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RQR6"/>
    <dbReference type="GeneID" id="108270539"/>
    <dbReference type="KEGG" id="ipu:108270539"/>
    <dbReference type="CTD" id="283951"/>
    <dbReference type="OMA" id="KGSHRTW"/>
    <dbReference type="OrthoDB" id="5783753at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029160">
        <property type="entry name" value="UQCC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023248">
        <property type="entry name" value="UQCC4_vert"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35268">
        <property type="entry name" value="PROTEIN CCSMST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35268:SF1">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME-C REDUCTASE COMPLEX ASSEMBLY FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15013">
        <property type="entry name" value="CCSMST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02042">
        <property type="entry name" value="CCSMST1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
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    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="43"/>
            <end position="70"/>
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    <feature type="region of interest" description="Disordered" evidence="12">
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            <begin position="131"/>
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            <begin position="58"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017332839.1"/>
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    <sequence length="155" mass="17737" checksum="3E730C5735E8DB20" modified="2017-12-20" version="1">MSAAGRCVFSGLLRYHSLSPGQIKPAILRPCIARSLSISSQLYDKPGRSPGSTDGEETLSQPIKFSTSKASHRTWKVERSMGSRFQRPWWKVLPISVFTIGFLLWCIFRQETDIDRALEKHLFEHLPGLLTNMEEEEEETEEREELKKEPDDGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RRA1</accession>
    <name>A0A2D0RRA1_ICTPU</name>
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        <submittedName>
            <fullName evidence="14">Echinoderm microtubule-associated protein-like 5 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">eml5</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
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    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat EMAP family.</text>
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        <property type="component" value="Chromosome 9"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Putative echinoderm microtubule-associated protein-like 6"/>
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        <property type="entry name" value="WD40_repeat_CS"/>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
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        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720:SF16">
        <property type="entry name" value="ECHINODERM MICROTUBULE-ASSOCIATED PROTEIN-LIKE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD-40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Beta-prop_EML"/>
        <property type="match status" value="3"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="TolB, C-terminal domain"/>
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    </dbReference>
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        <property type="entry name" value="WD40 repeat-like"/>
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    </dbReference>
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        <property type="entry name" value="WD_REPEATS_1"/>
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    </dbReference>
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        <property type="entry name" value="WD_REPEATS_2"/>
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    </dbReference>
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        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="EML-like first beta-propeller" evidence="11">
        <location>
            <begin position="53"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="250"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="12">
        <location>
            <begin position="328"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="330"/>
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        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="404"/>
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    </feature>
    <feature type="domain" description="EML-like first beta-propeller" evidence="11">
        <location>
            <begin position="719"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="896"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="12">
        <location>
            <begin position="998"/>
            <end position="1264"/>
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    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="1231"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like first beta-propeller" evidence="11">
        <location>
            <begin position="1404"/>
            <end position="1674"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="12">
        <location>
            <begin position="1694"/>
            <end position="1961"/>
        </location>
    </feature>
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        <location>
            <begin position="1700"/>
            <end position="1728"/>
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    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="1770"/>
            <end position="1811"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="1927"/>
            <end position="1959"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="610"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="615"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003210"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006489"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23409"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017332701.2"/>
        </source>
    </evidence>
    <sequence length="1966" mass="219370" checksum="40DFC3C6337300CC" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RRU7</accession>
    <name>A0A2D0RRU7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Speckle-type POZ protein-like B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">spoplb</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Tdpoz family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017333222.1">
        <property type="nucleotide sequence ID" value="XM_017477733.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RRU7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000014201"/>
    <dbReference type="GeneID" id="108270802"/>
    <dbReference type="KEGG" id="ipu:108270802"/>
    <dbReference type="CTD" id="558170"/>
    <dbReference type="OrthoDB" id="6359816at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18519">
        <property type="entry name" value="BACK_SPOPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03774">
        <property type="entry name" value="MATH_SPOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000028">
        <property type="entry name" value="Speckle-type POZ protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000008">
        <property type="entry name" value="Speckle-type POZ protein-like a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24413">
        <property type="entry name" value="SPECKLE-TYPE POZ PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="MATH" evidence="7">
        <location>
            <begin position="54"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="6">
        <location>
            <begin position="223"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017333222.1"/>
        </source>
    </evidence>
    <sequence length="415" mass="46945" checksum="EA7AE4545EE41054" modified="2017-12-20" version="1">MSNLSLSEHKVKLHKGSERIKLAMSRVPTPPPPGEMTSGPVAESWCYTQVKVVKFSYMWTINNFSFCREEMGEVLKSSTFSSGPNDKMKWCLRVNPKGLDDESKDYLSLYLLLVNCPKSEVRAKFKFSLLNAKREETKAMESQRAYRFVQGKDWGFKKFIRRDFLLDEANGLLPDDKLTLFCEVSVVQDSVNISGQSNMNMLKVPECQLADDLGSLWEGSRFTDCSLFVGGQEFKAHKSILAARSPVFNAMFEHKMEESQKNRVDISDVEPDVFREMMVFIYTDKAPNLEKMADHLLAAADKYALERLKVMCEEALCNSLSVENVADILILADLHSAEQLKAQAIDFINRCSVLRQLGCKDGKNWNSNHAADIMETAGWKAMIQSHPHLVAEAFRALASAQCPPFGLPRKRLKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RWM4</accession>
    <name>A0A2D0RWM4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Leucine-rich repeat and calponin homology domain-containing protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">lrch1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017334898.1">
        <property type="nucleotide sequence ID" value="XM_017479409.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RWM4"/>
    <dbReference type="GeneID" id="108271695"/>
    <dbReference type="KEGG" id="ipu:108271695"/>
    <dbReference type="CTD" id="23143"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000007">
        <property type="entry name" value="Leucine-rich repeat and calponin homology domain-containing protein 1 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF38">
        <property type="entry name" value="LEUCINE RICH REPEATS AND CALPONIN HOMOLOGY DOMAIN CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="5">
        <location>
            <begin position="573"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="268"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="433"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="493"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="692"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="563"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="277"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="433"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="493"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="692"/>
            <end position="708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017334898.1"/>
        </source>
    </evidence>
    <sequence length="750" mass="83128" checksum="ED1A3EDF68B79714" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RWY2</accession>
    <name>A0A2D0RWY2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">BCL6A transcription repressor a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">bcl6aa</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017335008.1">
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
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        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
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        <property type="entry name" value="Znf_C2H2_sf"/>
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        <property type="entry name" value="B-CELL LYMPHOMA 6 PROTEIN ISOFORM X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
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    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
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        <location>
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        <source>
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        <source>
            <dbReference type="RefSeq" id="XP_017335008.1"/>
        </source>
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    <sequence length="707" mass="79505" checksum="B6B6F6ABFC3CA544" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RYZ0</accession>
    <name>A0A2D0RYZ0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanine nucleotide-binding protein subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 13 14" type="primary">gng12b</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12 13 14" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12 13 14">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">G proteins are composed of 3 units; alpha, beta and gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Lipid-anchor</topology>
            <orientation evidence="1 9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the G protein gamma family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017479912.3"/>
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        <property type="nucleotide sequence ID" value="XM_017479913.3"/>
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        <property type="nucleotide sequence ID" value="XM_047158497.2"/>
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        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00068">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.260.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001770">
        <property type="entry name" value="G-protein_gamma"/>
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        <property type="entry name" value="G-protein_gamma-like_dom"/>
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    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13809">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00321">
        <property type="entry name" value="GPROTEING"/>
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    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00224">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50058">
        <property type="entry name" value="G_PROTEIN_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="G protein gamma" evidence="10">
        <location>
            <begin position="7"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017335401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017335402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_047014453.1"/>
        </source>
    </evidence>
    <sequence length="72" mass="8146" checksum="D7C5BD9829BD8B06" modified="2017-12-20" version="1">MSSKMQSSNNIAQARRIVQQLRIEANIERIKVSKASADLMHYCSEHAKYDPLLMGIPASENPFKDKKPCTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S1T0</accession>
    <name>A0A2D0S1T0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Interferon-induced very large GTPase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC108272484</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Very large inducible GTPase (VLIG) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017336401.1">
        <property type="nucleotide sequence ID" value="XM_017480912.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000001194"/>
    <dbReference type="GeneID" id="108272484"/>
    <dbReference type="KEGG" id="ipu:108272484"/>
    <dbReference type="OMA" id="WSGWITE"/>
    <dbReference type="OrthoDB" id="1597724at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030383">
        <property type="entry name" value="G_VLIG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058641">
        <property type="entry name" value="GVIN1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057365">
        <property type="entry name" value="URGCP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22796:SF6">
        <property type="entry name" value="INTERFERON-INDUCED VERY LARGE GTPASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22796">
        <property type="entry name" value="URG4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25496">
        <property type="entry name" value="URGCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25974">
        <property type="entry name" value="URGCP_9th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25683">
        <property type="entry name" value="URGCP_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51717">
        <property type="entry name" value="G_VLIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VLIG-type G" evidence="9">
        <location>
            <begin position="815"/>
            <end position="1059"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017336401.1"/>
        </source>
    </evidence>
    <sequence length="1788" mass="206663" checksum="AC25D05632730376" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S2X1</accession>
    <name>A0A2D0S2X1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cysteine protease</fullName>
            <ecNumber evidence="12">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC108272831</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[protein]-C-terminal L-amino acid-glycyl-phosphatidylethanolamide + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine</text>
            <dbReference type="Rhea" id="RHEA:67548"/>
            <dbReference type="Rhea" id="RHEA-COMP:17323"/>
            <dbReference type="Rhea" id="RHEA-COMP:17324"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:172940"/>
            <dbReference type="ChEBI" id="CHEBI:172941"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:67549"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[protein]-C-terminal L-amino acid-glycyl-phosphatidylserine + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phospho-L-serine</text>
            <dbReference type="Rhea" id="RHEA:67576"/>
            <dbReference type="Rhea" id="RHEA-COMP:17324"/>
            <dbReference type="Rhea" id="RHEA-COMP:17326"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:172940"/>
            <dbReference type="ChEBI" id="CHEBI:172942"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67577"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the peptidase C54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="12"/>
    <dbReference type="RefSeq" id="XP_017337103.1">
        <property type="nucleotide sequence ID" value="XM_017481614.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S2X1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032131"/>
    <dbReference type="GeneID" id="108272831"/>
    <dbReference type="KEGG" id="ipu:108272831"/>
    <dbReference type="OMA" id="SFHRMDP"/>
    <dbReference type="OrthoDB" id="2960936at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019786">
        <property type="term" value="F:protein-phosphatidylethanolamide deconjugating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005078">
        <property type="entry name" value="Peptidase_C54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046792">
        <property type="entry name" value="Peptidase_C54_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624:SF36">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03416">
        <property type="entry name" value="Peptidase_C54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 12 15" id="KW-0645">Protease</keyword>
    <keyword evidence="8 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase C54 catalytic" evidence="13">
        <location>
            <begin position="88"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017337103.1"/>
        </source>
    </evidence>
    <sequence length="455" mass="51418" checksum="DFC9D635DD7C0AD5" modified="2017-12-20" version="1">MTTVCPDEVNSTEDLESFEFLSHPEPTESTHSSEAVKDKARLKSRLVSAWNSVKYRGWTLKAKARLSKNSPVCMLGYTYHLSNEGEKQQFQCVFSSLFWMTYRRGFSSLHGSSLTSDSGWGCTLRSAQMLLAQALVLHTLPPGWTWPGARHQTRDDLELRHSRPLVLGLGRSQRVGKRRRSEGAILDEDEDWQESGHRRLVAWFGDTPSAPFSLHQLVELGRSSGQRAGDWFGPSFAAHMLRKAVAASEVSDLAVYVAQDCTVYVGDVMRLYGESRCELSLRFHSVIILVPVRLGGDTLNHVYVPYVKRLLQLKCCVGIIGGKPKHSLYFVGFQDDHLLYLDPHFCQAAVDVSQPNFPLESFHCKTARKLPFHRMDPSCTLGFYAKSKRDFETLRSDVTMALTSSTDTYPIFTFMEGSGQDQHERLELTLDPEVQVARKGRQRSKRSSTDEFVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S819</accession>
    <name>A0A2D0S819_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12 13">Probable endonuclease 4 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 12 13" type="primary">si:ch211-141o9.10</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11 12 13" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11 12 13">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AP endonuclease 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017338892.1">
        <property type="nucleotide sequence ID" value="XM_017483403.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017338893.1">
        <property type="nucleotide sequence ID" value="XM_017483404.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047015369.1">
        <property type="nucleotide sequence ID" value="XM_047159413.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S819"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000002069"/>
    <dbReference type="GeneID" id="108273827"/>
    <dbReference type="KEGG" id="ipu:108273827"/>
    <dbReference type="GeneTree" id="ENSGT00390000013698"/>
    <dbReference type="OMA" id="HPGSHLR"/>
    <dbReference type="OrthoDB" id="7663182at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00019">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.150:FF:000001">
        <property type="entry name" value="Probable endonuclease 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00152">
        <property type="entry name" value="Nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018246">
        <property type="entry name" value="AP_endonuc_F2_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00587">
        <property type="entry name" value="nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002199">
        <property type="entry name" value="PRK01060.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445:SF0">
        <property type="entry name" value="APURINIC-APYRIMIDINIC ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445">
        <property type="entry name" value="ENDONUCLEASE IV ENDODEOXYRIBONUCLEASE IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00729">
        <property type="entry name" value="AP_NUCLEASE_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00730">
        <property type="entry name" value="AP_NUCLEASE_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00731">
        <property type="entry name" value="AP_NUCLEASE_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51432">
        <property type="entry name" value="AP_NUCLEASE_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11 12 13" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12 13" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Xylose isomerase-like TIM barrel" evidence="9">
        <location>
            <begin position="69"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017338892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017338893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_047015369.1"/>
        </source>
    </evidence>
    <sequence length="332" mass="36577" checksum="669040C4056A2B40" modified="2017-12-20" version="1">MGPHKRGEKRRKERDLVKMEEVEDSAELGEPSEDGTEEMAREKKGRTEEQKKYIGAHVSIAGGIWKAVEASVDMGGHSFGLFLGSQRTWQRPALDQTAAVKFQQACTQHGFDPKQILPHGSYLMNCGSPKEDIFAKSQAMLVDELSRCSVLGLTQFNFHPGASMGSSKEQCFERIAQAINHAHQQIPAVCTVLENMSGQGSTVGGQFNELKSIIDHVRDKTRVGVCLDTCHAFAAGYDISSAGGVKSVLDEFDQVVGLHYLRAVHLNDSKGKLGCHLDRHEDIGRGKIGISAFRNIVNEPRLDNIPLILETPGRPGFEYAEQIKLLYSLCED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S8Q7</accession>
    <name>A0A2D0S8Q7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Semaphorin-4G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">sema4gb</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017339129.1">
        <property type="nucleotide sequence ID" value="XM_017483640.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S8Q7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022953"/>
    <dbReference type="GeneID" id="108273939"/>
    <dbReference type="KEGG" id="ipu:108273939"/>
    <dbReference type="CTD" id="100534852"/>
    <dbReference type="OrthoDB" id="9988752at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001170">
        <property type="entry name" value="Sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (Semaphorin) 3F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000033">
        <property type="entry name" value="Semaphorin 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF17">
        <property type="entry name" value="SEMAPHORIN-4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="705"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="17">
        <location>
            <begin position="40"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="791"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="800"/>
            <end position="815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017339129.1"/>
        </source>
    </evidence>
    <sequence length="882" mass="99508" checksum="51D7BBD6A337C5A4" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S9K1</accession>
    <name>A0A2D0S9K1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">small monomeric GTPase</fullName>
            <ecNumber evidence="2">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rac1a</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017338910.1">
        <property type="nucleotide sequence ID" value="XM_017483421.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S9K1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009334"/>
    <dbReference type="GeneID" id="108273830"/>
    <dbReference type="KEGG" id="ipu:108273830"/>
    <dbReference type="CTD" id="327204"/>
    <dbReference type="OMA" id="NITEHWI"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01871">
        <property type="entry name" value="Rac1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000088">
        <property type="entry name" value="Ras-related C3 botulinum toxin substrate 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017338910.1"/>
        </source>
    </evidence>
    <sequence length="192" mass="21352" checksum="345FA98FC770E49C" modified="2017-12-20" version="1">MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPMILVGTKIDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGSVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKPKPKCLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SBN8</accession>
    <name>A0A2D0SBN8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transmembrane protein 80</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC108274465</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017340142.1">
        <property type="nucleotide sequence ID" value="XM_017484653.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SBN8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012773"/>
    <dbReference type="GeneID" id="108274465"/>
    <dbReference type="KEGG" id="ipu:108274465"/>
    <dbReference type="OMA" id="LRIEYIW"/>
    <dbReference type="OrthoDB" id="262535at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019184">
        <property type="entry name" value="Uncharacterised_TM-17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13531">
        <property type="entry name" value="GEO07735P1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13531:SF8">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09799">
        <property type="entry name" value="Transmemb_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017340142.1"/>
        </source>
    </evidence>
    <sequence length="145" mass="16228" checksum="2EEA6F9284A1494B" modified="2017-12-20" version="1">MATRRTDRTASVLSSVLLQILLYASAFYSLFYFLSSLCMIIYKSQVLSYPDNYLVLDVCLLLLMAAFEILRVYWGVRGNLQESERYLGASVLITGATLLLSVYFLVWQSYVLRADVIINAILLCIYGLSGVAGFGALARFTSIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SCU0</accession>
    <name>A0A2D0SCU0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tyrosine 3-monooxygenase</fullName>
            <ecNumber evidence="8">1.14.16.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Tyrosine 3-hydroxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">th</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">SDA103</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Plays an important role in the physiology of adrenergic neurons.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + L-tyrosine + O2 = (4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin + L-dopa</text>
            <dbReference type="Rhea" id="RHEA:18201"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:57504"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:59560"/>
            <dbReference type="EC" id="1.14.16.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 21">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.16.2" evidence="8"/>
    <dbReference type="RefSeq" id="XP_017340286.1">
        <property type="nucleotide sequence ID" value="XM_017484797.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SCU0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007956"/>
    <dbReference type="GeneID" id="108274586"/>
    <dbReference type="KEGG" id="ipu:108274586"/>
    <dbReference type="CTD" id="7054"/>
    <dbReference type="OMA" id="SVEHGYP"/>
    <dbReference type="OrthoDB" id="983542at2759"/>
    <dbReference type="UniPathway" id="UPA00747">
        <property type="reaction ID" value="UER00733"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004511">
        <property type="term" value="F:tyrosine 3-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006585">
        <property type="term" value="P:dopamine biosynthetic process from tyrosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021522">
        <property type="term" value="P:spinal cord motor neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03345">
        <property type="entry name" value="eu_TyrOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.800.10:FF:000002">
        <property type="entry name" value="Tyrosine 3-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000024">
        <property type="entry name" value="Tyrosine 3-monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.800.10">
        <property type="entry name" value="Aromatic amino acid hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001273">
        <property type="entry name" value="ArAA_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018301">
        <property type="entry name" value="ArAA_hydroxylase_Fe/CU_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036951">
        <property type="entry name" value="ArAA_hydroxylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036329">
        <property type="entry name" value="Aro-AA_hydroxylase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019774">
        <property type="entry name" value="Aromatic-AA_hydroxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041903">
        <property type="entry name" value="Eu_TyrOH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049321">
        <property type="entry name" value="TH_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005962">
        <property type="entry name" value="Tyr_3_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019773">
        <property type="entry name" value="Tyrosine_3-monooxygenase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021164">
        <property type="entry name" value="Tyrosine_hydroxylase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01269">
        <property type="entry name" value="Tyr_3_monoox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11473">
        <property type="entry name" value="AROMATIC AMINO ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11473:SF39">
        <property type="entry name" value="TYROSINE 3-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00351">
        <property type="entry name" value="Biopterin_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21417">
        <property type="entry name" value="TH_ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12549">
        <property type="entry name" value="TOH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000336">
        <property type="entry name" value="TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00372">
        <property type="entry name" value="FYWHYDRXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56534">
        <property type="entry name" value="Aromatic aminoacid monoxygenases, catalytic and oligomerization domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00367">
        <property type="entry name" value="BH4_AAA_HYDROXYL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51410">
        <property type="entry name" value="BH4_AAA_HYDROXYL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0127">Catecholamine biosynthesis</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16 20" id="KW-0408">Iron</keyword>
    <keyword evidence="13 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="14" id="KW-0530">Neurotransmitter biosynthesis</keyword>
    <keyword evidence="15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biopterin-dependent aromatic amino acid hydroxylase family profile" evidence="23">
        <location>
            <begin position="143"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00009712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00011993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00017181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00032379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000336-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR601273-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_017340286.1"/>
        </source>
    </evidence>
    <sequence length="489" mass="55862" checksum="71D82D2040CE0ABA" modified="2017-12-20" version="1">MPNSTSSTSSTKSMRRAGSELERSDSITSQRFVGRRQSLIEDARKEREAAEAAEAAAEANEHIVFEEDDGKALLNLFFTLRGSKSPGLSRTLKVFETFEAKMHHLETRLSRKPKEGPQDLEYFVRCEVHLSDVNTLVSSLRRIAEDVRTTKEVKFHWFPKKIAELDRCHHLVTKFDPDLDQDHPGFTDPLYIKRRKLIGDIAFKYKHGETIPRVEYTQEEIQTWREVYNTLRDLYTTHACNEHLEAFRLLEKHCGYSPNSIPQLEDVSCFLKERTGFQLRPVAGLLSARDFLASLAFRVFQCTQYIRHASSPMHSPEPDCVHELLGHVPMLADKTFAQFSQNIGLASLGASEDDIEKLSTLYWFTVEFGLCKQGDTVKAYGAGLLSSYGELMHALSDDTERREFDPDTVAVQPYQDQTYQSVYFVSESFSDAKDKLRMYSMGIKRPFSVRFDPYTNSIEVLDNPLKIRGGLEMVKEELKVLADALNILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SHY8</accession>
    <name>A0A2D0SHY8_ICTPU</name>
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        <submittedName>
            <fullName evidence="8">Calcium-independent phospholipase A2-gamma isoform X1</fullName>
        </submittedName>
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        <name evidence="8" type="primary">LOC108275530</name>
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    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_017486406.3"/>
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    <dbReference type="STRING" id="7998.ENSIPUP00000017741"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:calcium-independent phospholipase A2 activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:lipid catabolic process"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    </dbReference>
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        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
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        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185">
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    </dbReference>
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        <property type="entry name" value="Patatin"/>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        </location>
    </feature>
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        </location>
    </feature>
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        <source>
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        <source>
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        </source>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51635"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017341895.1"/>
        </source>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
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                <person name="Liu H."/>
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                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
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        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7 8">Blood</tissue>
        </source>
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    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    </dbReference>
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</entry>
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    <name>A0A2D0SPR0_ICTPU</name>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
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                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
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            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="7">Blood</tissue>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017344270.1"/>
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    </evidence>
    <sequence length="1005" mass="114209" checksum="E61A6942429B63B3" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SQ95</accession>
    <name>A0A2D0SQ95_ICTPU</name>
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            <taxon>Neopterygii</taxon>
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    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
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    </reference>
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    </dbReference>
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    <dbReference type="GeneID" id="108276974"/>
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    <dbReference type="OMA" id="LVTYIHW"/>
    <dbReference type="OrthoDB" id="6251307at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:galactoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="Galectin-4"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
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        </location>
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        <location>
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        </location>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU102079"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017344671.1"/>
        </source>
    </evidence>
    <sequence length="322" mass="35656" checksum="8E1E1433A23450C8" modified="2017-12-20" version="1">MVILHCITMSCQRPYLNPRLPFTGCIQGGLFEGKTVTVTGRVLAGAERFCVNLQSGTKANSDIILHFNPRYSGNSGHVVCNTLQSSCWGSEQCSKHTPLPRGSDFTLTFLVNRDSYSVIVNGVHFLEYLHRLPASRVNAISVNGGVEITSITFQNPATSWVAQPSFARTVKASYAVHGLGATPPPYSPPLAYVLPYKAIIQGGIYPGRVITVQGCVNHNADRFNINLRYNSGIAFHFDPRFNENVVVRNSLINECWGREERSGGMPFYRGQQFTVSITCDTQCYRIAVNGAHMFTYNHRHFLFQQIDILEVDGNISVSSVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SWT0</accession>
    <name>A0A2D0SWT0_ICTPU</name>
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        <submittedName>
            <fullName evidence="8">Nephrin isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nphs1</name>
    </gene>
    <organism evidence="7 8">
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        <name type="synonym">Silurus punctatus</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017491280.3"/>
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        <property type="component" value="Chromosome 17"/>
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        <property type="entry name" value="nephrin isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="nephrin isoform X1"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="10"/>
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        <property type="entry name" value="CD80_C2-set"/>
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        <property type="entry name" value="Cell_adhesion_signaling"/>
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        <property type="entry name" value="FN3_dom"/>
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        <property type="entry name" value="FN3_sf"/>
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    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
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    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
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    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
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    <dbReference type="PANTHER" id="PTHR11640">
        <property type="entry name" value="NEPHRIN"/>
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        <property type="entry name" value="NEPHRIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="4"/>
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    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
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    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="IG"/>
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        <property type="entry name" value="IGc2"/>
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        <property type="entry name" value="IG_LIKE"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="3" id="KW-0472">Membrane</keyword>
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    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017346769.1"/>
        </source>
    </evidence>
    <sequence length="1237" mass="136349" checksum="DC42ED9F84C039B1" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SZ93</accession>
    <name>A0A2D0SZ93_ICTPU</name>
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        <submittedName>
            <fullName evidence="15">Protein Shroom3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">shroom3</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the shroom family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017492171.3"/>
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    <dbReference type="KEGG" id="ipu:108278690"/>
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    <dbReference type="OrthoDB" id="10063560at2759"/>
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        <property type="component" value="Chromosome 18"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0043296">
        <property type="term" value="C:apical junction complex"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:apical plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="CDD" id="cd06750">
        <property type="entry name" value="PDZ_shroom2_3_4-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.30.42.10:FF:000100">
        <property type="entry name" value="Shroom family member 2"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3120">
        <property type="match status" value="1"/>
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        <property type="entry name" value="PDZ"/>
    </dbReference>
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        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027685">
        <property type="entry name" value="Shroom_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15012">
        <property type="entry name" value="APICAL PROTEIN/SHROOM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15012:SF33">
        <property type="entry name" value="PROTEIN SHROOM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08688">
        <property type="entry name" value="ASD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08687">
        <property type="entry name" value="ASD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ASD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="11">
        <location>
            <begin position="15"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="ASD1" evidence="12">
        <location>
            <begin position="844"/>
            <end position="951"/>
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    </feature>
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        <location>
            <begin position="1497"/>
            <end position="1783"/>
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            <begin position="123"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
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            <begin position="189"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017347660.1"/>
        </source>
    </evidence>
    <sequence length="1811" mass="199905" checksum="95B59B2AC779C955" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T150</accession>
    <name>A0A2D0T150_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">SH2 domain-containing protein 4A isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">zgc:100829</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4 5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017348516.2">
        <property type="nucleotide sequence ID" value="XM_017493027.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053543778.1">
        <property type="nucleotide sequence ID" value="XM_053687803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T150"/>
    <dbReference type="GeneID" id="108279072"/>
    <dbReference type="GeneTree" id="ENSGT00940000157357"/>
    <dbReference type="OMA" id="PPMRANA"/>
    <dbReference type="OrthoDB" id="10003345at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14388:SF5">
        <property type="entry name" value="SH2 DOMAIN-CONTAINING PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14388">
        <property type="entry name" value="T CELL-SPECIFIC ADAPTER PROTEIN TSAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0727">SH2 domain</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="101"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="280"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="178"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="287"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017348516.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053543778.1"/>
        </source>
    </evidence>
    <sequence length="328" mass="36855" checksum="F69D0BB99C9E28B7" modified="2023-06-28" version="2">MLQQILKEMYIDPDVLDALNEEQKKTLFLKMRQEQVRRWNEREEKLEHEEPRKPKQRPAQSKSVSWLLGCDGDVQVIVIGEMDEFKSSKILRSGFGERKAASLRNNSHNQEASLKSNLVSRTSTEPVRSGRENLPPKSLSGVQLNLRENSEEVRTLPPQQVSAGEQKPASPVESVKETEEEDDTNDPVDDTLSLSSICYRPHLMSSSVLHSLAPQDTQQKTTPLSITSRLCSVEDSEAHISRGKDFRVMSAFKRTESGVGGETRMGRGRVAELMKTFSVSSDSTCSQPPPRANKPPIPNKPSHLQHLPSNSFSAAWRTCSVWVERQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T6J5</accession>
    <name>A0A2D0T6J5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein mono-ADP-ribosyltransferase PARP11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC108280019</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017350244.1">
        <property type="nucleotide sequence ID" value="XM_017494755.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T6J5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013269"/>
    <dbReference type="GeneID" id="108280019"/>
    <dbReference type="KEGG" id="ipu:108280019"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990404">
        <property type="term" value="F:NAD+-protein mono-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01439">
        <property type="entry name" value="TCCD_inducible_PARP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051712">
        <property type="entry name" value="ARTD-AVP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004170">
        <property type="entry name" value="WWE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037197">
        <property type="entry name" value="WWE_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45740">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45740:SF4">
        <property type="entry name" value="PROTEIN MONO-ADP-RIBOSYLTRANSFERASE PARP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02825">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117839">
        <property type="entry name" value="WWE domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50918">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017350244.1"/>
        </source>
    </evidence>
    <sequence length="345" mass="40480" checksum="795BD52534A2756B" modified="2017-12-20" version="1">MPLPQMHCSVTDKCVQHVQQMCPKNNDEESCEEEMDTTEPNWHWFYLAECGVWHMFEIDPSAGCSVTSEQIEANYNRNQHGSMDFYTPKYSYRLDFSVMKQINVTTGKQRPIKRALHSATGFRFICDNLALPVPCHWERVNTDEPYQLIPLCRDTFEYKEVARLYERTMSQPIKSIQRIQNLDLWEFFCRKKAQLRKIKRIIDIDERMLFHGTGHSNLQAICTYNFDWRLTGSHGDVYGKGSYFARDAKYSSKFCHSTSKHNFTLQRHGLAPPIFQSDPPYKCMFLARVLVGEYTVGHPHFCRPPSKDLSIANFFDSCVDDMVNPKIFVIFDSNQIYPEYLIEFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T7T8</accession>
    <name>A0A2D0T7T8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">exo-alpha-sialidase</fullName>
            <ecNumber evidence="3">3.2.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC108280464</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of alpha-(2-&gt;3)-, alpha-(2-&gt;6)-, alpha-(2-&gt;8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.</text>
            <dbReference type="EC" id="3.2.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 33 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.18" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017350944.1">
        <property type="nucleotide sequence ID" value="XM_017495455.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T7T8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000010934"/>
    <dbReference type="GeneID" id="108280464"/>
    <dbReference type="KEGG" id="ipu:108280464"/>
    <dbReference type="OMA" id="FICVEKY"/>
    <dbReference type="OrthoDB" id="2739686at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004308">
        <property type="term" value="F:exo-alpha-sialidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006689">
        <property type="term" value="P:ganglioside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009313">
        <property type="term" value="P:oligosaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15482">
        <property type="entry name" value="Sialidase_non-viral"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011040">
        <property type="entry name" value="Sialidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026856">
        <property type="entry name" value="Sialidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036278">
        <property type="entry name" value="Sialidase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10628">
        <property type="entry name" value="SIALIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10628:SF23">
        <property type="entry name" value="SIALIDASE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13088">
        <property type="entry name" value="BNR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50939">
        <property type="entry name" value="Sialidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sialidase" evidence="8">
        <location>
            <begin position="41"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017350944.1"/>
        </source>
    </evidence>
    <sequence length="371" mass="41943" checksum="891D70C37926F711" modified="2017-12-20" version="1">MGSCVSKSSSHTPETNSSETPLFTNTIYRIPALIYINHDQTLLAFAEKRTSLNDADATLLVMRRGTQQNGSVQWSSVQELTTARLDKYRTMNPCPVYERQSKTVFLFFNCVFGKVSERDQISKRKNQARLCHVTSRDSGQTWSDVTDLTESVIGNEMKNWATFSVSPGHGIQMNDGRLIIPAYVYYIHCLVVKPYAFAFYSDDGGATWHYGRRVSVQSTECQMAEITKDDGKSSLYCNARGNKSCQNHRVEAVSKNRGVDFETFSQKLVELPYGCQGSVVSFQHGGKTCLLFSHTTDKKKRKDLGVYLNMTPDKVSGWKKPHIINPGVSGYSDMTRRGEEEERFACLMERGTTDLEEIAFKEFSLHEIMNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T9W1</accession>
    <name>A0A2D0T9W1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Coronin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">coro1cb</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Regulates leading edge dynamics and cell motility in fibroblasts. May be involved in cytokinesis and signal transduction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Forms homooligomers, but does not form complexes with the other coronins. Interacts with Arp2/3 complex components, including ACTR2, ARPC1B and ARPC2. Binds actin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Stress fiber</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the WD repeat coronin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017351659.1">
        <property type="nucleotide sequence ID" value="XM_017496170.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T9W1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000017995"/>
    <dbReference type="GeneID" id="108280777"/>
    <dbReference type="KEGG" id="ipu:108280777"/>
    <dbReference type="CTD" id="562849"/>
    <dbReference type="OrthoDB" id="1850764at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000003">
        <property type="entry name" value="Coronin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015505">
        <property type="entry name" value="Coronin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015048">
        <property type="entry name" value="DUF1899"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856">
        <property type="entry name" value="CORONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856:SF24">
        <property type="entry name" value="CORONIN-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08953">
        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16300">
        <property type="entry name" value="WD40_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01166">
        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01167">
        <property type="entry name" value="DUF1900"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 12 13" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="DUF1899" evidence="16">
        <location>
            <begin position="408"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="481"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="531"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="86"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="178"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="838"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="25"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="247"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU280818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017351659.1"/>
        </source>
    </evidence>
    <sequence length="878" mass="99145" checksum="C1A68DFA9A8F2F3B" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QXY3</accession>
    <name>A0A9F7QXY3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10 11">NLR family CARD domain-containing protein 3-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10 11 12" type="primary">LOC128630838</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10 11" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10 11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053535099.1">
        <property type="nucleotide sequence ID" value="XM_053679124.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053535100.1">
        <property type="nucleotide sequence ID" value="XM_053679125.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053535101.1">
        <property type="nucleotide sequence ID" value="XM_053679126.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053535102.1">
        <property type="nucleotide sequence ID" value="XM_053679127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QXY3"/>
    <dbReference type="GeneID" id="128630838"/>
    <dbReference type="KEGG" id="ipu:128630838"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000210">
        <property type="entry name" value="Si:dkey-16p6.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="7">
        <location>
            <begin position="99"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_053535099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053535100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053535101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053535102.1"/>
        </source>
    </evidence>
    <sequence length="625" mass="71424" checksum="A5FA0EC281536943" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QYN2</accession>
    <name>A0A9F7QYN2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC108280324</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108280324</name>
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    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053529702.1">
        <property type="nucleotide sequence ID" value="XM_053673727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QYN2"/>
    <dbReference type="GeneID" id="108280324"/>
    <dbReference type="KEGG" id="ipu:108280324"/>
    <dbReference type="OrthoDB" id="8936769at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053529702.1"/>
        </source>
    </evidence>
    <sequence length="88" mass="9603" checksum="AC93CB7F895D2471" modified="2023-06-28" version="1">MLEDECVKLVKDLSTKKESRDLSVLSVATPLVGASVFITLLLLSFAMFKRQESQAGGVLGSLIHYPAHSLERTTNPGAFLPPETKIPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QYT4</accession>
    <name>A0A9F7QYT4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PIH1 domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 9 10" type="primary">pih1d1</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8 9 10" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8 9 10">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in the assembly of C/D box small nucleolar ribonucleoprotein (snoRNP) particles. Recruits the SWI/SNF complex to the core promoter of rRNA genes and enhances pre-rRNA transcription. Mediates interaction of TELO2 with the R2TP complex which is necessary for the stability of MTOR and SMG1. Positively regulates the assembly and activity of the mTORC1 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PIH1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053532925.1">
        <property type="nucleotide sequence ID" value="XM_053676950.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053532929.1">
        <property type="nucleotide sequence ID" value="XM_053676954.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053532934.1">
        <property type="nucleotide sequence ID" value="XM_053676959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QYT4"/>
    <dbReference type="GeneID" id="128628642"/>
    <dbReference type="KEGG" id="ipu:128628642"/>
    <dbReference type="CTD" id="55011"/>
    <dbReference type="OrthoDB" id="5135119at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097255">
        <property type="term" value="C:R2TP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050734">
        <property type="entry name" value="PIH1/Kintoun_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012981">
        <property type="entry name" value="PIH1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041442">
        <property type="entry name" value="PIH1D1/2/3_CS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22997">
        <property type="entry name" value="PIH1 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22997:SF0">
        <property type="entry name" value="PIH1 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08190">
        <property type="entry name" value="PIH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18201">
        <property type="entry name" value="PIH1_CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIH1 N-terminal" evidence="5">
        <location>
            <begin position="31"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="PIH1D1/2/3 CS-like" evidence="6">
        <location>
            <begin position="209"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00046233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053532925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_053532929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053532934.1"/>
        </source>
    </evidence>
    <sequence length="350" mass="39815" checksum="047D44E8F370F5B4" modified="2023-06-28" version="1">METDTSLLRAELEEEELYNLLQSVGKTLTDSMPSKVIRPQPGLCLKTVSLPDQQKLFVNICQSADVPPPPPISHDALVELFESDDPTVYKVPMSLGEAHTEMDNSSNSCTVYDVIINEEFFQKCQKDSLFQQFVISVSLEGLENKYKLQINRDIKILKNRKFMGSLTEQNVRMKSKPVIQEISSEETQSQLATVKRPQVCLVVEPPVGQVEYLIAEVQLPGVSSVRSLVLDLGEDRLVLNARPSLFHLDIFLPFLIDQDNSGAQYNTDTQCWSSFCPRCCANPPVAVCRICCYPACIKRPSLFPTTIICTSTERERERERERERVFHPQQRPPRLTKDLHTSTDRNMIMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QZ55</accession>
    <name>A0A9F7QZ55_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Malic enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">me2</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">NAD-dependent mitochondrial malic enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(S)-malate + NAD(+) = pyruvate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:12653"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.38"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>oxaloacetate + H(+) = pyruvate + CO2</text>
            <dbReference type="Rhea" id="RHEA:15641"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="EC" id="1.1.1.38"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="17">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="17">Divalent metal cations. Prefers magnesium or manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the malic enzymes family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053530535.1">
        <property type="nucleotide sequence ID" value="XM_053674560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QZ55"/>
    <dbReference type="GeneID" id="108255419"/>
    <dbReference type="KEGG" id="ipu:108255419"/>
    <dbReference type="CTD" id="4200"/>
    <dbReference type="OrthoDB" id="5365701at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004471">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004473">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05312">
        <property type="entry name" value="NAD_bind_1_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10380:FF:000006">
        <property type="entry name" value="Malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000060">
        <property type="entry name" value="Malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015884">
        <property type="entry name" value="Malic_enzyme_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010052">
        <property type="entry name" value="PRK13529.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406">
        <property type="entry name" value="MALIC ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406:SF27">
        <property type="entry name" value="NAD-DEPENDENT MALIC ENZYME, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00072">
        <property type="entry name" value="MALOXRDTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00331">
        <property type="entry name" value="MALIC_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="6 17 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="9 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="20">
        <location>
            <begin position="117"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="19">
        <location>
            <begin position="308"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="493"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_053530535.1"/>
        </source>
    </evidence>
    <sequence length="609" mass="68266" checksum="590505FBA4276344" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R628</accession>
    <name>A0A9F7R628_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein ofcc1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ofcc1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053530666.1">
        <property type="nucleotide sequence ID" value="XM_053674691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R628"/>
    <dbReference type="GeneID" id="128628983"/>
    <dbReference type="KEGG" id="ipu:128628983"/>
    <dbReference type="CTD" id="266553"/>
    <dbReference type="OrthoDB" id="347244at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031390">
        <property type="entry name" value="OFCC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33862">
        <property type="entry name" value="OROFACIAL CLEFT 1 CANDIDATE GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33862:SF3">
        <property type="entry name" value="OROFACIAL CLEFT 1 CANDIDATE GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="326"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="382"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053530666.1"/>
        </source>
    </evidence>
    <sequence length="590" mass="66678" checksum="52524D24E51238DC" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RC52</accession>
    <name>A0A9F7RC52_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Dynein axonemal heavy chain 11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Axonemal beta dynein heavy chain 11</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Ciliary dynein heavy chain 11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="primary">dnah9l</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="31">SDA103</strain>
        </source>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="32">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Consists of at least two heavy chains and a number of intermediate and light chains. Interacts with CFAP45.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053540040.1">
        <property type="nucleotide sequence ID" value="XM_053684065.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108272446"/>
    <dbReference type="CTD" id="552915"/>
    <dbReference type="OrthoDB" id="10251809at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097729">
        <property type="term" value="C:9+2 motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.280:FF:000008">
        <property type="entry name" value="Dynein axonemal heavy chain 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000667">
        <property type="entry name" value="Dynein axonemal heavy chain 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2620:FF:000004">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000003">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.30:FF:000003">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.20:FF:000002">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000219">
        <property type="entry name" value="Dynein axonemal heavy chain 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1220:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.180.20:FF:000001">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002141">
        <property type="entry name" value="Dynein heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.710:FF:000002">
        <property type="entry name" value="dynein heavy chain 17, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.100:FF:000001">
        <property type="entry name" value="dynein heavy chain 17, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000411">
        <property type="entry name" value="dynein heavy chain 17, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.720:FF:000002">
        <property type="entry name" value="Dynein heavy chain 9, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1120:FF:000002">
        <property type="entry name" value="Dynein heavy chain 9, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000049">
        <property type="entry name" value="Dynein, axonemal, heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.130:FF:000001">
        <property type="entry name" value="Dynein, axonemal, heavy chain 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041589">
        <property type="entry name" value="DNAH3_AAA_lid_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041228">
        <property type="entry name" value="Dynein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043160">
        <property type="entry name" value="Dynein_C_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013594">
        <property type="entry name" value="Dynein_heavy_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703:SF12">
        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17857">
        <property type="entry name" value="AAA_lid_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08385">
        <property type="entry name" value="DHC_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18199">
        <property type="entry name" value="Dynein_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="9 19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Dynein heavy chain tail" evidence="21">
        <location>
            <begin position="204"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain linker" evidence="22">
        <location>
            <begin position="1283"/>
            <end position="1690"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain hydrolytic ATP-binding dynein motor region" evidence="23">
        <location>
            <begin position="1824"/>
            <end position="2150"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA 5 extension" evidence="27">
        <location>
            <begin position="2308"/>
            <end position="2426"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain 3 AAA+ lid" evidence="28">
        <location>
            <begin position="2642"/>
            <end position="2738"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA module D4" evidence="25">
        <location>
            <begin position="2785"/>
            <end position="3044"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain coiled coil stalk" evidence="24">
        <location>
            <begin position="3057"/>
            <end position="3401"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain ATP-binding dynein motor region" evidence="26">
        <location>
            <begin position="3428"/>
            <end position="3645"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain region D6 P-loop" evidence="20">
        <location>
            <begin position="3886"/>
            <end position="4006"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain AAA lid" evidence="29">
        <location>
            <begin position="4038"/>
            <end position="4174"/>
        </location>
    </feature>
    <feature type="domain" description="Dynein heavy chain C-terminal" evidence="30">
        <location>
            <begin position="4182"/>
            <end position="4475"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="3049"/>
            <end position="3076"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="3108"/>
            <end position="3135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00062166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00069441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00082501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF08385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF08393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF12774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF12777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF12780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF12781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF17852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF17857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="Pfam" id="PF18198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="Pfam" id="PF18199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="RefSeq" id="XP_053540040.1"/>
        </source>
    </evidence>
    <sequence length="4477" mass="514015" checksum="AA4A0A3DC4B93F59" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RD67</accession>
    <name>A0A9F7RD67_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">C-terminal PDZ ligand of neuronal nitric oxide synthase protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Nitric oxide synthase 1 adaptor protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">si:dkey-34e4.1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Adapter protein involved in neuronal nitric-oxide (NO) synthesis regulation via its association with nNOS/NOS1. The complex formed with NOS1 and synapsins is necessary for specific NO and synapsin functions at a presynaptic level. Mediates an indirect interaction between NOS1 and RASD1 leading to enhance the ability of NOS1 to activate RASD1. Competes with DLG4 for interaction with NOS1, possibly affecting NOS1 activity by regulating the interaction between NOS1 and DLG4. In kidney podocytes, plays a role in podosomes and filopodia formation through CDC42 activation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053533078.1">
        <property type="nucleotide sequence ID" value="XM_053677103.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RD67"/>
    <dbReference type="GeneID" id="108259774"/>
    <dbReference type="KEGG" id="ipu:108259774"/>
    <dbReference type="OMA" id="MDEKYKQ"/>
    <dbReference type="OrthoDB" id="10030336at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050998">
        <property type="term" value="F:nitric-oxide synthase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01270">
        <property type="entry name" value="PTB_CAPON-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000124">
        <property type="entry name" value="carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051133">
        <property type="entry name" value="Adapter_Engulfment-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232:SF80">
        <property type="entry name" value="CARBOXYL-TERMINAL PDZ LIGAND OF NEURONAL NITRIC OXIDE SYNTHASE PROTEIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11232">
        <property type="entry name" value="PHOSPHOTYROSINE INTERACTION DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PID" evidence="8">
        <location>
            <begin position="29"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="415"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="451"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="503"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="275"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="188"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="425"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="512"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="531"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00054402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00067706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00075003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00075107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053533078.1"/>
        </source>
    </evidence>
    <sequence length="603" mass="66422" checksum="CDB8FE28B5468393" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RG67</accession>
    <name>A0A9F7RG67_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein LOC108272161 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC108272161</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053539572.1">
        <property type="nucleotide sequence ID" value="XM_053683597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RG67"/>
    <dbReference type="GeneID" id="108272161"/>
    <dbReference type="OrthoDB" id="5817083at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20893">
        <property type="entry name" value="LD08641P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20893:SF2">
        <property type="entry name" value="LD08641P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="6">
        <location>
            <begin position="23"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="209"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="225"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="274"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053539572.1"/>
        </source>
    </evidence>
    <sequence length="285" mass="33501" checksum="AF0728A1B23DC1BC" modified="2023-06-28" version="1">MLERVQEDVKAMMDDGCADTCEVLIAETPECFICRDVQLVDAEQLKRFCDCKNLMAHHGCLLTWVRTGLKRNDQLRCRVCNAEYQQQRVAQWQTLVSQWRVWPVVLLAVVLVALIPYTVYRLLKAFNNPPPHILFNMAAICFGILSEILLLSKTPKWGLRPRVRKKPSSLLNLFGTEGGGEAKINLVVGGFVVARRYAHLWLSNCGDDNKPCDSHHPQTSTTLLHQREREREREREREREREREREREREREREREREREREREREREIDLASYISSSNQNPCFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RGJ4</accession>
    <name>A0A9F7RGJ4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Proprotein convertase subtilisin/kexin type 5b isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">pcsk5b</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13 14">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053536098.1">
        <property type="nucleotide sequence ID" value="XM_053680123.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108265616"/>
    <dbReference type="CTD" id="559556"/>
    <dbReference type="OrthoDB" id="300641at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="CDD" id="cd04059">
        <property type="entry name" value="Peptidases_S8_Protein_convertases_Kexins_Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000011">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000006">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.850:FF:000001">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000002">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.220.10">
        <property type="entry name" value="Hormone Receptor, Insulin-like Growth Factor Receptor 1, Chain A, domain 2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.850">
        <property type="entry name" value="Peptidase S8, pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032778">
        <property type="entry name" value="GF_recep_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034182">
        <property type="entry name" value="Kexin/furin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002884">
        <property type="entry name" value="P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043601">
        <property type="entry name" value="Rspo_Fu-CRD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032815">
        <property type="entry name" value="S8_pro-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038466">
        <property type="entry name" value="S8_pro-domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF7">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15913">
        <property type="entry name" value="Furin-like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14843">
        <property type="entry name" value="GF_recep_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01483">
        <property type="entry name" value="P_proprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16470">
        <property type="entry name" value="S8_pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51829">
        <property type="entry name" value="P_HOMO_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 13 14" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 13 14" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1756"/>
            <end position="1780"/>
        </location>
    </feature>
    <feature type="domain" description="P/Homo B" evidence="16">
        <location>
            <begin position="466"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="12 13">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="12 13">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="12 13">
        <location>
            <position position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_053536098.1"/>
        </source>
    </evidence>
    <sequence length="1870" mass="207476" checksum="E03A528F142938F5" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RMD7</accession>
    <name>A0A9F7RMD7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Centrosomal protein of 41 kDa isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC108268419</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the CEP41 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053538043.1">
        <property type="nucleotide sequence ID" value="XM_053682068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RMD7"/>
    <dbReference type="GeneID" id="108268419"/>
    <dbReference type="OrthoDB" id="70250at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
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                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
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                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
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                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
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                <person name="Kirby M.L."/>
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</entry>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
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                <person name="Danzmann R.G."/>
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                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6 7 8" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6 7 8">Blood</tissue>
        </source>
    </reference>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_053687902.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_053687903.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_053687904.1"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="568"/>
            <end position="621"/>
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    <feature type="domain" description="G-patch" evidence="4">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="2">
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        <location>
            <begin position="812"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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    <evidence type="ECO:0000313" key="6">
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    <sequence length="841" mass="95274" checksum="092084B674FD2C43" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RQE1</accession>
    <name>A0A9F7RQE1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">scn1lab</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">SDA103</strain>
        </source>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="28">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="function">
        <text evidence="19">Pore-forming subunit of a voltage-gated sodium (Nav) channel that directly mediates the depolarizing phase of action potentials in excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na+ ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="20">Voltage-gated sodium (Nav) channels consist of an ion-conducting alpha subunit which is functional on its own associated with regulatory beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 21">Cell membrane</location>
            <topology evidence="1 21">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.4/SCN4A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053540267.1">
        <property type="nucleotide sequence ID" value="XM_053684292.1"/>
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        <property type="component" value="Chromosome 12"/>
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    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
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    <dbReference type="CDD" id="cd13433">
        <property type="entry name" value="Na_channel_gate"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ion_trans_dom"/>
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        <property type="entry name" value="IQ_SCN5A_C"/>
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        <property type="entry name" value="Na_channel_asu"/>
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        <property type="entry name" value="Na_chnl_inactivation_gate"/>
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        <property type="entry name" value="Na_trans_cytopl"/>
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        <property type="entry name" value="VGCC_Ca_Na"/>
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        <property type="entry name" value="Volt_channel_dom_sf"/>
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    <dbReference type="PANTHER" id="PTHR10037:SF278">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN TYPE 2 SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
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    </dbReference>
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        <property type="entry name" value="IQ_SCN5A_C"/>
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        <property type="entry name" value="Na_trans_assoc"/>
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        <property type="entry name" value="Na_trans_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00170">
        <property type="entry name" value="NACHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 21 28" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11 21" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10 21" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 21" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="15 21" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 21" id="KW-0813">Transport</keyword>
    <keyword evidence="8 21" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
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            <begin position="252"/>
            <end position="272"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="402"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="756"/>
            <end position="774"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="786"/>
            <end position="809"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="821"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="866"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="956"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="1213"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="1252"/>
            <end position="1271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="1283"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="1329"/>
            <end position="1355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="1452"/>
            <end position="1475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="1534"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="1564"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="1594"/>
            <end position="1614"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="1651"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="1755"/>
            <end position="1778"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="23">
        <location>
            <begin position="129"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="Voltage-gated Na+ ion channel cytoplasmic" evidence="25">
        <location>
            <begin position="556"/>
            <end position="705"/>
        </location>
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    <feature type="domain" description="Ion transport" evidence="23">
        <location>
            <begin position="755"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium ion transport-associated" evidence="24">
        <location>
            <begin position="993"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="23">
        <location>
            <begin position="1211"/>
            <end position="1484"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="23">
        <location>
            <begin position="1533"/>
            <end position="1788"/>
        </location>
    </feature>
    <feature type="domain" description="SCN5A-like C-terminal IQ motif" evidence="26">
        <location>
            <begin position="1900"/>
            <end position="1934"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="29"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="465"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="663"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1117"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1958"/>
            <end position="2007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="465"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="481"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="22">
        <location>
            <begin position="497"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="517"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="1967"/>
            <end position="1981"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="1982"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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            <dbReference type="RuleBase" id="RU361132"/>
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            <dbReference type="Pfam" id="PF24609"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_053540267.1"/>
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    <sequence length="2007" mass="227542" checksum="E3921B877FD62177" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
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                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
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            <tissue evidence="3">Blood</tissue>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Ostariophysi</taxon>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
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    <reference evidence="3 4 5" key="2">
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            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3 4 5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053542741.1">
        <property type="nucleotide sequence ID" value="XM_053686766.1"/>
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        <property type="nucleotide sequence ID" value="XM_053686806.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053542812.1">
        <property type="nucleotide sequence ID" value="XM_053686837.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053542834.1">
        <property type="nucleotide sequence ID" value="XM_053686859.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053542855.1">
        <property type="nucleotide sequence ID" value="XM_053686880.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053542895.1">
        <property type="nucleotide sequence ID" value="XM_053686920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RS41"/>
    <dbReference type="GeneID" id="108262770"/>
    <dbReference type="KEGG" id="ipu:108262770"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053542741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053542781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053542812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_053542834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053542855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053542895.1"/>
        </source>
    </evidence>
    <sequence length="229" mass="25264" checksum="500D502BB5D306AD" modified="2023-06-28" version="1">MKKNKNKERGARSGSKWITRLWKTMWVRTTEDLTRMVFSPSLWRQLSAGRLFPPALVKTTALRKLKARVEGPSLSSRPHGGETRRSEGQMDVFTPAGGGGGWREEEEEEEEEEEEETKRLPPLSFRVVLLPSSSRERNSTFHPSVRSRLTFSSNSGVTGNGQHARDRSPLCSAGDAADLLLTDPGKSRGAGDSESEHAAAPASFSFYFSSLSLAWTLDGEPENSGLARI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RTQ5</accession>
    <name>A0A9F7RTQ5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">tm9sf4</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Chen A."/>
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                <person name="Yuan Z."/>
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            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Geng X."/>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11">SDA103</strain>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF133">
        <property type="entry name" value="IMMUNE-TYPE RECEPTOR 5 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039930938" evidence="9">
        <location>
            <begin position="21"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="29"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053538276.1"/>
        </source>
    </evidence>
    <sequence length="267" mass="30201" checksum="D5A31C1BAA199BA0" modified="2023-06-28" version="1">MTGFWILILIFSTMFTVQPGRRWVTAQSPTESPVYQPDKELSVDIGDTDTLQCCVSKNIFGMIYLFKQPNRKNPQLIVKFYKNAGETFYNGFQESRFEIERSSNCFNMTILNIVQSDEAMYYCALTSPNTVFADGTYLKIKGDHVTIASETSKPALCDNSVVCEPTLHGNSTNMNTHEKTVLGLGTALGLCSLLIFCLIYFILREENVIKVINASIEDCSGMRQKSEAETLNYAALKFPKRKARAEKRKTGSSYECVYSDVKYNFKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TP80</accession>
    <name>A0A9F7TP80_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WD repeat-containing protein 37</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">wdr37</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053540001.1">
        <property type="nucleotide sequence ID" value="XM_053684026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TP80"/>
    <dbReference type="GeneID" id="108272441"/>
    <dbReference type="KEGG" id="ipu:108272441"/>
    <dbReference type="CTD" id="22884"/>
    <dbReference type="OrthoDB" id="9984207at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000511">
        <property type="entry name" value="WD repeat domain 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000080">
        <property type="entry name" value="WD repeat-containing protein 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000152">
        <property type="entry name" value="WD repeat-containing protein 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855:SF12">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855">
        <property type="entry name" value="WD40 REPEAT PROTEIN 12, 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="195"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="277"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="319"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="363"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="237"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="69"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053540001.1"/>
        </source>
    </evidence>
    <sequence length="494" mass="54751" checksum="5D4AB132C164A490" modified="2023-06-28" version="1">MPVESGNSAVARQAKQKRKSHSLSIRRTNSTEQERPSIHRDMLEAQDSKLPMALRSTLLDLFGQIEREFENLYLENIELRREIESLNERLAGEGQTVDGGDLGKGALKTKASHSTSQLSQKLKTTYKASTSKIVSSFKATTSRAVCQLVKEYVGHRDGIWDLSVTRTQPLVLGTASADHCAMLWSIETGKCLLKYAGHAGSVNSIKFHPTEQMALTASGDQTAHIWRYMVQLPLPQPPSDTSASLDDDVDFSDKDDADGDADGPTECPTVRLASTTLKSHQGVVIAADWLVGGKQVVTASWDRAANLYDVETSELVHTLTGHDQELTHCCTHPTQRLVVTSSRDTTFRLWDFRDPSIHSVNVFQGHTDTVTSAVFTVGDNVVSGSDDRTVKVWDLKNMRSPIATIRTDSAVNRISVSANQRIIALPHDNRQVRLFDMTGVRLARLPRSNRQGHRRMVCCSAWNEENQACNLFTCGFDRQAIGWNINIPALLQDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3TDP5</accession>
    <name>E3TDP5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RNA polymerase II subunit A C-terminal domain phosphatase SSU72</fullName>
            <shortName evidence="10">CTD phosphatase SSU72</shortName>
            <ecNumber evidence="10">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SSU72</name>
        <name evidence="13" type="synonym">ssu72</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7 10">Protein phosphatase that catalyzes the dephosphorylation of the C-terminal domain of RNA polymerase II. Plays a role in RNA processing and termination.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SSU72 phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="10"/>
    <dbReference type="EMBL" id="GU588475">
        <property type="protein sequence ID" value="ADO28431.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187275.1">
        <property type="nucleotide sequence ID" value="NM_001200346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TDP5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026798"/>
    <dbReference type="GeneID" id="100528076"/>
    <dbReference type="KEGG" id="ipu:100528076"/>
    <dbReference type="CTD" id="29101"/>
    <dbReference type="GeneTree" id="ENSGT00390000010165"/>
    <dbReference type="OMA" id="PNCYEFG"/>
    <dbReference type="OrthoDB" id="57957at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008420">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000039">
        <property type="entry name" value="RNA polymerase II subunit A C-terminal domain phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000066">
        <property type="entry name" value="RNA polymerase II subunit A C-terminal domain phosphatase SSU72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036196">
        <property type="entry name" value="Ptyr_pPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006811">
        <property type="entry name" value="RNA_pol_II_suA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20383">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT A C-TERMINAL DOMAIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04722">
        <property type="entry name" value="Ssu72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52788">
        <property type="entry name" value="Phosphotyrosine protein phosphatases I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 10" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 10" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ADO28431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="NP_001187275.1"/>
        </source>
    </evidence>
    <sequence length="194" mass="22659" checksum="534355ABE91ECFBA" modified="2011-01-11" version="1">MPSHPLRVAVVCSSNQNRSMEAHNILSKRGFDVRSFGTGSHVKLPGPAPDKPNVYDFKTTYEQMYSDLVRKDKELYTQNGILHMLERNKRIKTRPERFQSCKDQFDLVITCEERVYDQVLEDLNSREQETFQPVHVINVDIQDNHEEATLGAFLICELCQCIQHTDDMENDIDELLQEFEDKSSRPFLHTVCFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3TDU5</accession>
    <name>E3TDU5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4 6">Retinol dehydrogenase 13</fullName>
            <ecNumber evidence="4">1.1.1.105</ecNumber>
            <ecNumber evidence="6">1.1.1.300</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">RDH13</name>
        <name evidence="6" type="synonym">rdh13</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.105" evidence="4"/>
    <dbReference type="EC" id="1.1.1.300" evidence="6"/>
    <dbReference type="EMBL" id="GU588525">
        <property type="protein sequence ID" value="ADO28481.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187312.1">
        <property type="nucleotide sequence ID" value="NM_001200383.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TDU5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000619"/>
    <dbReference type="GeneID" id="100528113"/>
    <dbReference type="KEGG" id="ipu:100528113"/>
    <dbReference type="CTD" id="112724"/>
    <dbReference type="OMA" id="LMCATEP"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004745">
        <property type="term" value="F:all-trans-retinol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052650">
        <property type="term" value="F:all-trans-retinol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF59">
        <property type="entry name" value="RETINOL DEHYDROGENASE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADO28481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="NP_001187312.1"/>
        </source>
    </evidence>
    <sequence length="357" mass="39040" checksum="4DD8102687AB81F8" modified="2011-01-11" version="1">MSRYILPASIFGTVFGGAVLLKNHLTGGTCPSKARIPGKTVVITGANTGIGKETARELARRGGRIIMGCRDMGKCEEAAREIRGSTLNPHVYARHVDLASIKSIRSFAEKINQEEERVDILINNAAVMRCPPGKTEDGFDMQLGVNYLGHFLLTNLLLDKLRDSAPSRVINLSSLAHIIGEIDFEDLNWDKKMFNTKKAYCQSKLAIVLFTRELARRLEGTGITVNALHPGVVATELGRHTGMHQSQFSSTVLSPFFYLLIKSPELGAQPSVYLAVAEELTSVSGRYYDVMKEKEPAPQALDQEVAVKLWDISASLVGLETTVSVPTSSPETVTPVKVLPEEIRTYQSEPTAALTNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3TDY5</accession>
    <name>E3TDY5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Chitinase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CHID1</name>
        <name evidence="12" type="synonym">chid1</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <dbReference type="EMBL" id="GU588565">
        <property type="protein sequence ID" value="ADO28521.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187344.1">
        <property type="nucleotide sequence ID" value="NM_001200415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TDY5"/>
    <dbReference type="GeneID" id="100528145"/>
    <dbReference type="KEGG" id="ipu:100528145"/>
    <dbReference type="CTD" id="66005"/>
    <dbReference type="OrthoDB" id="10254444at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070492">
        <property type="term" value="F:oligosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02876">
        <property type="entry name" value="GH18_SI-CLP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.10:FF:000002">
        <property type="entry name" value="Chitinase domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000028">
        <property type="entry name" value="Chitinase domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.360">
        <property type="entry name" value="3,6-anhydro-alpha-l-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46066:SF2">
        <property type="entry name" value="CHITINASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46066">
        <property type="entry name" value="CHITINASE DOMAIN-CONTAINING PROTEIN 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0458">Lysosome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013522785" description="Chitinase domain-containing protein 1" evidence="8">
        <location>
            <begin position="20"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="9">
        <location>
            <begin position="78"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ADO28521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="NP_001187344.1"/>
        </source>
    </evidence>
    <sequence length="390" mass="44002" checksum="CBA143B817499EB4" modified="2011-01-11" version="1">MRAVLIFLYISTSVPGIRGTLSKTDAKKLSKVPEAETAIAEQPVQERGLVVTTLQWRDVVKEERRFCPHAISTRHFQGTVLGYVTPWNSHGYDIAKVFGPKLTSVSPVWLQIRRKGPESFHITGLHDNDQGWVKAVKKANKKSKILPRLLFDGWSYQDYMSVLDSEDEIEEIGREIVQVAKAEGFDGYTLELWSQLGGNKRTELVHLVTHLCKYLKAGKFSCILVIPPSVIPGTNQPGMFGREDFEKLAPVVDAFSLMTYDYSGPGRPGPSSPLPWMRDCVLQLAPQSQWRYKILLGLNLYGLDFTANGGGEPLLGGRYVEILKEVKPKLVWDEYAGEHYFNYKRSSVKHAVYYPTLKSLQLRVELATELGTGISLWELGQGLDYFYDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3TEA6</accession>
    <name>E3TEA6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein FAM33A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">SKA2</name>
        <name evidence="17" type="synonym">ska2</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 17" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2016" name="Biol. Reprod." volume="95" first="19" last="19">
            <title>Transcriptome Display During Testicular Differentiation of Channel Catfish (Ictalurus punctatus) as Revealed by RNA-Seq Analysis.</title>
            <authorList>
                <person name="Zeng Q."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Zhang Y."/>
                <person name="Yuan Z."/>
                <person name="Jiang C."/>
                <person name="Chen A."/>
                <person name="Fu Q."/>
                <person name="Su B."/>
                <person name="Dunham R."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="27307075"/>
            <dbReference type="DOI" id="10.1095/biolreprod.116.138818"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SKA2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GU588686">
        <property type="protein sequence ID" value="ADO28642.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187439.1">
        <property type="nucleotide sequence ID" value="NM_001200510.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TEA6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027242"/>
    <dbReference type="GeneID" id="100528240"/>
    <dbReference type="KEGG" id="ipu:100528240"/>
    <dbReference type="CTD" id="348235"/>
    <dbReference type="GeneTree" id="ENSGT00390000009588"/>
    <dbReference type="OMA" id="AMFQKAD"/>
    <dbReference type="OrthoDB" id="193920at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000940">
        <property type="term" value="C:outer kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12955">
        <property type="entry name" value="SKA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026762">
        <property type="entry name" value="Ska2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042091">
        <property type="entry name" value="Ska2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32017">
        <property type="entry name" value="SPINDLE AND KINETOCHORE-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32017:SF3">
        <property type="entry name" value="SPINDLE AND KINETOCHORE-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16740">
        <property type="entry name" value="SKA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="12" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ska2 N-terminal" evidence="14">
        <location>
            <begin position="2"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF16740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ADO28642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="NP_001187439.1"/>
        </source>
    </evidence>
    <sequence length="226" mass="25379" checksum="604E869D932127BA" modified="2011-01-11" version="1">METAVDKLEAMFQKAEADIEYVEKRLKFDFMTSARENSTAEGNPVQLLESLAAIKARHGALCVQMEEIAAEQKRSMDSIRSHLNTTVKLVEELQNTTDMQVPPLTQEEQEARDSLCSSITALSIKTQCEVHTQQESKSQEKCRDVKDAEFEAVPRSVRGNIKLSDLNALYTQLTEHFSSDNNRAPLSLQKMRKLNMRVSEAAVKTLMHLGLVELDRKGSVSLPSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3TEF9</accession>
    <name>E3TEF9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Actin-related protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ARP6</name>
        <name evidence="10" type="synonym">actr6</name>
        <name evidence="10" type="synonym">arp6</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the actin family. ARP6 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="GU588741">
        <property type="protein sequence ID" value="ADO28695.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187481.1">
        <property type="nucleotide sequence ID" value="NM_001200552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TEF9"/>
    <dbReference type="GeneID" id="100528282"/>
    <dbReference type="KEGG" id="ipu:100528282"/>
    <dbReference type="CTD" id="64431"/>
    <dbReference type="OrthoDB" id="6220758at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd10210">
        <property type="entry name" value="ASKHA_NBD_Arp6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.36.70:FF:000003">
        <property type="entry name" value="Actin-related protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000014">
        <property type="entry name" value="Putative actin-related protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.36.70">
        <property type="entry name" value="Actin, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADO28695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="NP_001187481.1"/>
        </source>
    </evidence>
    <sequence length="396" mass="46044" checksum="5A7D446649DFF5C4" modified="2011-01-11" version="1">MRTLILDNGAYTAKIGYSHEKVSVIPNCQFRSKSLRLKTFTANQLDEIRDPSGLFYILPFQKGYLVNWDVQRKVWDQLFGKEMFKVDFADTSIVITEPYFNFTSIQESMNEILFEEYQFQAALRINAGSLSAHRHFHDYNSELCCIVVDSGFSFTHIIPYCRGRKMKDGICRINVGGKLLTNHLKEIISYRQLHVMDETYVINQVKEDVCYVSQEFYEDMQIAQLKGEENLVMRDYVLPDYSSIKKGFCKPREEMNFTGKYKTGEQILRLTNERFVVPEMLFHPSDIGIQEMGIPEAIVNSINKMPEEMQPHFFKNIVLTGGNTLFPGFRDRVYKDVRALAPSEFDVSVIQPQNPICYPWEGGKLLAENPDIEEMMVTREDYEENGHFVCEENFDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3TEU3</accession>
    <name>E3TEU3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">10 kDa heat shock protein, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">10 kDa chaperonin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Chaperonin 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CH10</name>
        <name evidence="11" type="synonym">ch10</name>
        <name evidence="11" type="synonym">hspe1</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Co-chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp60, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homoheptamer arranged in a ring structure. 2 heptameric Hsp10 rings interact with a Hsp60 tetradecamer in the structure of a back-to-back double heptameric ring to form the symmetrical football complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the GroES chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="GU588875">
        <property type="protein sequence ID" value="ADO28829.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001188078.1">
        <property type="nucleotide sequence ID" value="NM_001201149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TEU3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019265"/>
    <dbReference type="GeneID" id="100528879"/>
    <dbReference type="KEGG" id="ipu:100528879"/>
    <dbReference type="CTD" id="3336"/>
    <dbReference type="OMA" id="EDFLIMR"/>
    <dbReference type="OrthoDB" id="184876at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.33.40:FF:000002">
        <property type="entry name" value="10 kDa chaperonin, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.33.40">
        <property type="entry name" value="GroES chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00580">
        <property type="entry name" value="CH10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037124">
        <property type="entry name" value="Chaperonin_GroES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772:SF0">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00297">
        <property type="entry name" value="CHAPERONIN10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3 8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0346">Stress response</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ADO28829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="NP_001188078.1"/>
        </source>
    </evidence>
    <sequence length="101" mass="10992" checksum="B2D1083D8E30423C" modified="2011-01-11" version="1">MAKAFRKFLPMFDRVLVERLAAETVTKGGIMIPEKSQGKVLQATVVAVGPGTTTKNGTVTPVCVNVGEKVLLPEYGGTKVVLEDKDYFLFRDADILGKYVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3TF62</accession>
    <name>E3TF62_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Nuclear nucleic acid-binding protein C1D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">C1D</name>
        <name evidence="9" type="synonym">c1d</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role in the recruitment of the exosome to pre-rRNA to mediate the 3'-5' end processing of the 5.8S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer and homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the C1D family.</text>
    </comment>
    <dbReference type="EMBL" id="GU588996">
        <property type="protein sequence ID" value="ADO28948.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187659.1">
        <property type="nucleotide sequence ID" value="NM_001200730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TF62"/>
    <dbReference type="GeneID" id="100528460"/>
    <dbReference type="KEGG" id="ipu:100528460"/>
    <dbReference type="CTD" id="10438"/>
    <dbReference type="OrthoDB" id="1421013at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011082">
        <property type="entry name" value="Exosome-assoc_fac/DNA_repair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007146">
        <property type="entry name" value="Sas10/Utp3/C1D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15341:SF3">
        <property type="entry name" value="NUCLEAR NUCLEIC ACID-BINDING PROTEIN C1D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15341">
        <property type="entry name" value="SUN-COR STEROID HORMONE RECEPTOR CO-REPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04000">
        <property type="entry name" value="Sas10_Utp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0698">rRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADO28948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="NP_001187659.1"/>
        </source>
    </evidence>
    <sequence length="143" mass="15939" checksum="DC30FEA9D3775882" modified="2011-01-11" version="1">MADEDSLGDYPSEIAEYLTGFVSSVGSVQNVIQTLMSVSKGDSLKLGTLEQAKLDLMSVYALNSLFWMYLVTQGVNPKDHGIKQELERIRTNMNRIKEITDKKQAACLDKDAASRFVRNALWVGKDTKPKDSMGKHSKPRKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3TF72</accession>
    <name>E3TF72_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mth938 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CK067</name>
        <name evidence="7 8" type="synonym">aamdc</name>
        <name evidence="7 8" type="synonym">ck067</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="journal article" date="2016" name="Biol. Reprod." volume="95" first="19" last="19">
            <title>Transcriptome Display During Testicular Differentiation of Channel Catfish (Ictalurus punctatus) as Revealed by RNA-Seq Analysis.</title>
            <authorList>
                <person name="Zeng Q."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Zhang Y."/>
                <person name="Yuan Z."/>
                <person name="Jiang C."/>
                <person name="Chen A."/>
                <person name="Fu Q."/>
                <person name="Su B."/>
                <person name="Dunham R."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="27307075"/>
            <dbReference type="DOI" id="10.1095/biolreprod.116.138818"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="5">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AAMDC family.</text>
    </comment>
    <dbReference type="EMBL" id="GU589006">
        <property type="protein sequence ID" value="ADO28958.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001188166.1">
        <property type="nucleotide sequence ID" value="NM_001201237.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017320593.1">
        <property type="nucleotide sequence ID" value="XM_017465104.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TF72"/>
    <dbReference type="GeneID" id="100528967"/>
    <dbReference type="KEGG" id="ipu:100528967"/>
    <dbReference type="CTD" id="28971"/>
    <dbReference type="OrthoDB" id="413520at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045600">
        <property type="term" value="P:positive regulation of fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05126">
        <property type="entry name" value="Mth938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1230.10:FF:000001">
        <property type="entry name" value="Adipogenesis-associated, Mth938 domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1230.10">
        <property type="entry name" value="MTH938-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034096">
        <property type="entry name" value="AAMDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036748">
        <property type="entry name" value="MTH938-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007523">
        <property type="entry name" value="NDUFAF3/AAMDC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15811">
        <property type="entry name" value="MTH938 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15811:SF5">
        <property type="entry name" value="MTH938 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04430">
        <property type="entry name" value="DUF498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64076">
        <property type="entry name" value="MTH938-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00061510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00074293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADO28958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="NP_001188166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017320593.1"/>
        </source>
    </evidence>
    <sequence length="122" mass="13295" checksum="1437589A0673A2D1" modified="2011-01-11" version="1">MSSPEIASLSWGHMKVKGCASSYKDCKVWPGGSREWDWRETGTDHYPGVQPADLEEILRKGVQTLVIGRGMSEALQVPPSTVEHVKKQGVDVKVLQTEKAVKEYNILAGQGAKVGGVFHSTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3TFQ9</accession>
    <name>E3TFQ9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phosphomannomutase</fullName>
            <ecNumber evidence="14">5.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">PMM2</name>
        <name evidence="17" type="synonym">pmm2</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 17" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="journal article" date="2016" name="Biol. Reprod." volume="95" first="19" last="19">
            <title>Transcriptome Display During Testicular Differentiation of Channel Catfish (Ictalurus punctatus) as Revealed by RNA-Seq Analysis.</title>
            <authorList>
                <person name="Zeng Q."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Zhang Y."/>
                <person name="Yuan Z."/>
                <person name="Jiang C."/>
                <person name="Chen A."/>
                <person name="Fu Q."/>
                <person name="Su B."/>
                <person name="Dunham R."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="27307075"/>
            <dbReference type="DOI" id="10.1095/biolreprod.116.138818"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="16" key="4">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="5">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2 14">Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>alpha-D-mannose 1-phosphate = D-mannose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11140"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="EC" id="5.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 14">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 14">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 14">Belongs to the eukaryotic PMM family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.8" evidence="14"/>
    <dbReference type="EMBL" id="GU589193">
        <property type="protein sequence ID" value="ADO29145.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187806.1">
        <property type="nucleotide sequence ID" value="NM_001200877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TFQ9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035792"/>
    <dbReference type="GeneID" id="100528607"/>
    <dbReference type="KEGG" id="ipu:100528607"/>
    <dbReference type="CTD" id="5373"/>
    <dbReference type="OrthoDB" id="10264771at2759"/>
    <dbReference type="UniPathway" id="UPA00126">
        <property type="reaction ID" value="UER00424"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004615">
        <property type="term" value="F:phosphomannomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061728">
        <property type="term" value="P:GDP-mannose biosynthetic process from mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02585">
        <property type="entry name" value="HAD_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1240.20:FF:000001">
        <property type="entry name" value="Phosphomannomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000216">
        <property type="entry name" value="Phosphomannomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005002">
        <property type="entry name" value="PMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043169">
        <property type="entry name" value="PMM_cap"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10466">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10466:SF2">
        <property type="entry name" value="PHOSPHOMANNOMUTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03332">
        <property type="entry name" value="PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00445">
        <property type="entry name" value="alpha-phosphomannomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01143">
        <property type="entry name" value="C2.B.3:_Phosphomannomutase_Lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01140">
        <property type="entry name" value="C2.B:_Phosphomannomutase_and_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 14 15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="11">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ADO29145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="NP_001187806.1"/>
        </source>
    </evidence>
    <sequence length="250" mass="28615" checksum="379638C65F73DB4D" modified="2011-01-11" version="1">MTSSGVDTETLCLFDVDGTLTAARQRATPHMQEFLSKLRQRVRVGVVGGSDLDKIKEQLGEDVIQRVDYVFAENGLVAYRFGQLHSVQSIQAYLGEEVLQDFINFCLDYMAKIKLPKKRGTFIEFRNGMLNVSPIGRSCSQQERIEFYELDKKEKIREKFVAVLKEEFRGRGLAFSIGGQISFDVFPEGWDKRYCLGIVEKDVYQTIHFFGDKTKPGGNDYEIYTDPRTVGHEVTSPDDTQRICEELFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-02-22" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>G9I934</accession>
    <name>G9I934_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">type I protein arginine methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.319</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15 17" type="primary">prmt1</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2011-08" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Yeh H.-Y."/>
                <person name="Klesius P.H."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="15 17" key="3">
        <citation type="journal article" date="2012" name="Fish Physiol. Biochem." volume="38" first="1083" last="1098">
            <title>Molecular characterization, phylogenetic analysis and expression patterns of five protein arginine methyltransferase genes of channel catfish, Ictalurus punctatus (Rafinesque).</title>
            <authorList>
                <person name="Yeh H.Y."/>
                <person name="Klesius P.H."/>
            </authorList>
            <dbReference type="PubMed" id="22286871"/>
            <dbReference type="DOI" id="10.1007/s10695-011-9593-x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="17" key="4">
        <citation type="journal article" date="2016" name="Biol. Reprod." volume="95" first="19" last="19">
            <title>Transcriptome Display During Testicular Differentiation of Channel Catfish (Ictalurus punctatus) as Revealed by RNA-Seq Analysis.</title>
            <authorList>
                <person name="Zeng Q."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Zhang Y."/>
                <person name="Yuan Z."/>
                <person name="Jiang C."/>
                <person name="Chen A."/>
                <person name="Fu Q."/>
                <person name="Su B."/>
                <person name="Dunham R."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="27307075"/>
            <dbReference type="DOI" id="10.1095/biolreprod.116.138818"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="16" key="5">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="6">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48096"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11991"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61897"/>
            <dbReference type="EC" id="2.1.1.319"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:48097"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-arginyl-[protein] + S-adenosyl-L-methionine = N(omega)-methyl-L-arginyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11990"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65280"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:48101"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(omega)-methyl-L-arginyl-[protein] + S-adenosyl-L-methionine = N(omega),N(omega)-dimethyl-L-arginyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48104"/>
            <dbReference type="Rhea" id="RHEA-COMP:11990"/>
            <dbReference type="Rhea" id="RHEA-COMP:11991"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61897"/>
            <dbReference type="ChEBI" id="CHEBI:65280"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:48105"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.319" evidence="3"/>
    <dbReference type="EMBL" id="JN617878">
        <property type="protein sequence ID" value="AEU08944.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001244040.1">
        <property type="nucleotide sequence ID" value="NM_001257111.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="G9I934"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009561"/>
    <dbReference type="GeneID" id="100862737"/>
    <dbReference type="KEGG" id="ipu:100862737"/>
    <dbReference type="CTD" id="3276"/>
    <dbReference type="OrthoDB" id="7848332at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035242">
        <property type="term" value="F:protein-arginine omega-N asymmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035241">
        <property type="term" value="F:protein-arginine omega-N monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.160.11:FF:000001">
        <property type="entry name" value="Blast:Protein arginine N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000003">
        <property type="entry name" value="Blast:Protein arginine N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055135">
        <property type="entry name" value="PRMT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF54">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22528">
        <property type="entry name" value="PRMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 12 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="13">
        <location>
            <begin position="82"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Protein arginine N-methyltransferase" evidence="14">
        <location>
            <begin position="184"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF22528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AEU08944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="NP_001244040.1"/>
        </source>
    </evidence>
    <sequence length="361" mass="41527" checksum="4C03D46A2D1A7445" modified="2012-02-22" version="1">MAETADTMEVSCGYTKSGPVKLVSQGESSEKPAAEDMTSKDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMFHNKHLFKDKVVLDVGSGTGILCMFAAKAGAKKVIGIECSSISDYAVKIVKANKLDHIVTIIKGKVEEVELPVDRVDIIISEWMGYCLFYESMLNTVIYARDKWLKPDGLIFPDRATLYITAIEDRQYKDYKIHWWENVYGFDMSCIKEVAIKEPLVDVVDPKQLVSSSCLIKEVDIYTVKIEDLSFTSPFCLQVKRNDYIHALVTYFNIEFTRCHKRTGFSTGPESPYTHWKQTVFYLDDYLTVKTGEEIFGTISMKPNIKNNRDLDFTVDIDFKGQLCEMSKTSEYRMR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-08-31" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>Q90YT6</accession>
    <name>RL32_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Large ribosomal subunit protein eL32</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>60S ribosomal protein L32</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">rpl32</name>
    </gene>
    <organism>
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2003" name="Gene" volume="305" first="151" last="160">
            <title>Translational machinery of channel catfish: II. Complementary DNA and expression of the complete set of 47 60S ribosomal proteins.</title>
            <authorList>
                <person name="Patterson A."/>
                <person name="Karsi A."/>
                <person name="Feng J."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="12609735"/>
            <dbReference type="DOI" id="10.1016/S0378-1119(02)01183-6"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [MRNA]</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the large ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eL32 family.</text>
    </comment>
    <dbReference type="EMBL" id="AF401587">
        <property type="protein sequence ID" value="AAK95159.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187056.1">
        <property type="nucleotide sequence ID" value="NM_001200127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q90YT6"/>
    <dbReference type="SMR" id="Q90YT6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012704"/>
    <dbReference type="GeneID" id="100304545"/>
    <dbReference type="KEGG" id="ipu:100304545"/>
    <dbReference type="CTD" id="6161"/>
    <dbReference type="OrthoDB" id="268693at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00513">
        <property type="entry name" value="Ribosomal_L32_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001515">
        <property type="entry name" value="Ribosomal_eL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018263">
        <property type="entry name" value="Ribosomal_eL32_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036351">
        <property type="entry name" value="Ribosomal_eL32_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L32 AND DNA-DIRECTED RNA POLYMERASE II, SUBUNIT N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01655">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01393">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52042">
        <property type="entry name" value="Ribosomal protein L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00580">
        <property type="entry name" value="RIBOSOMAL_L32E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000131119" description="Large ribosomal subunit protein eL32">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P62910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="135" mass="15696" checksum="713DC12033DF2EBB" modified="2001-12-01" version="1">MAALRPLTKPKIVKKRTKKFIRHQSDRYVKIRRNWRKPRGIDNRVRSGFKGQMLMPNIGYGSNKKTKHMLPSGFKKFLVHNVKELEVLLMSNKSYCAEIAHNVSSKNRKVIVERAAQLAIKVTNPNARLRSEENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5U691</accession>
    <name>W5U691_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">THO complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">Thoc3</name>
        <name evidence="18" type="synonym">thoc3</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. Required for efficient export of polyadenylated RNA and spliced mRNA. The THOC1-THOC2-THOC3 core complex alone is sufficient to bind export factor NXF1-NXT1 and promote ATPase activity of DDX39B. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NXF1 pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the THO subcomplex, which is composed of THOC1, THOC2, THOC3, THOC5, THOC6 and THOC7. The THO subcomplex interacts with DDX39B to form the THO-DDX39B complex which multimerizes into a 28-subunit tetrameric assembly. Component of the transcription/export (TREX) complex at least composed of ALYREF/THOC4, DDX39B, SARNP/CIP29, CHTOP and the THO subcomplex; in the complex interacts with THOC2. TREX seems to have a dynamic structure involving ATP-dependent remodeling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the THOC3 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT405721">
        <property type="protein sequence ID" value="AHH37295.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017330507.1">
        <property type="nucleotide sequence ID" value="XM_017475018.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U691"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000001828"/>
    <dbReference type="GeneID" id="108269258"/>
    <dbReference type="KEGG" id="ipu:108269258"/>
    <dbReference type="CTD" id="84321"/>
    <dbReference type="OrthoDB" id="340259at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000122">
        <property type="entry name" value="THO complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000166">
        <property type="entry name" value="THO complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040132">
        <property type="entry name" value="Tex1/THOC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22839:SF0">
        <property type="entry name" value="THO COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22839">
        <property type="entry name" value="THO COMPLEX SUBUNIT 3 THO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25174">
        <property type="entry name" value="Beta-prop_THOC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="3 15" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="22"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="66"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="191"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00062045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AHH37295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017330507.1"/>
        </source>
    </evidence>
    <sequence length="322" mass="36296" checksum="0E2B0CAA9D5D9074" modified="2014-04-16" version="1">MASSYYHEMHENFKNNSKSREFPAHSAKVHSVAWSCDGKRLASGSFDKTASVFVLEKDRLVKENNYRGHGDSVDQLCWHPTNPDLFVTASGDKTIRIWDVRTTKCMATVSTKGENINICWSPDGQTIAVGNKDDVVTFIDAKTHRPRAEEQFKFEVNEISWNNDNDMFFLTNGNGCINILSYPELKLIQSINAHPSNCICIKFDPTGKYFATGSADALVSLWDVEELVCVRCFSRLDWPVRTLSFSHDGKMLASASEDHFIDIAEVETGEKLWEVQCESPTFTVAWHPKRPLLAYACDDKEGKYDSNREAGTVKLFGLPNDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5U7L2</accession>
    <name>W5U7L2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Beta-2-glycoprotein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Apolipoprotein H</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Beta-2-glycoprotein I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">APOH</name>
        <name evidence="19" type="synonym">ntd5</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="JT407299">
        <property type="protein sequence ID" value="AHH37943.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017342716.1">
        <property type="nucleotide sequence ID" value="XM_017487227.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U7L2"/>
    <dbReference type="GeneID" id="108275909"/>
    <dbReference type="KEGG" id="ipu:108275909"/>
    <dbReference type="CTD" id="386628"/>
    <dbReference type="GeneTree" id="ENSGT00940000154967"/>
    <dbReference type="OMA" id="YCRARCL"/>
    <dbReference type="OrthoDB" id="6103690at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030414">
        <property type="term" value="F:peptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000014">
        <property type="entry name" value="Membrane cofactor protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050350">
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        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
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        <property type="entry name" value="Sushi_2"/>
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    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
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        <property type="entry name" value="WAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19325:SF502">
        <property type="entry name" value="BETA-2-GLYCOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19325">
        <property type="entry name" value="COMPLEMENT COMPONENT-RELATED SUSHI DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09014">
        <property type="entry name" value="Sushi_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00095">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51390">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 14" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0768">Sushi</keyword>
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        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
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            <begin position="24"/>
            <end position="358"/>
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    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="205"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00003651"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
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            <dbReference type="ARBA" id="ARBA00020104"/>
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            <dbReference type="ARBA" id="ARBA00022525"/>
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            <dbReference type="ARBA" id="ARBA00022729"/>
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            <dbReference type="PROSITE" id="PS51390"/>
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            <dbReference type="EMBL" id="AHH37943.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017342716.1"/>
        </source>
    </evidence>
    <sequence length="358" mass="39859" checksum="21D68233833DE41A" modified="2014-04-16" version="1">MEISQLLLLLCVWALSSLSLQSAEMIEHCPDRILDNEKRRTCPKKCQQDNDCGNKRQCLCDGPCGLSCVAPGRTCPWPLPSSEHFNVTLLSPSPSFSALLEVRCHPGYTMTNGLNATIRRCQGDRQWSGDDPRCTGRNAETEPESKLACPLPDHDLLLIHGSAAVGSTIQYQCAPGSVLVGSSENFCHKDQTWRYPQPVCQRVFCPPPQEVNHGYLVAVQKSEYNMGDTIYYLCKKNFLLDGPNQVTCQLDGTWSTVPSCRARCPIPAQRSRVIVGRVKSWPYDLTDGMVTHGENVTFFCKHPQKSCSFTATETCFDGLLPAPSCYLEPTWFQYKLYPHRLVSEINPCDPAGLDSTSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>W5U842</accession>
    <name>W5U842_ICTPU</name>
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        <submittedName>
            <fullName evidence="9">Mitochondrial sodium/hydrogen exchanger 9B2</fullName>
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        <submittedName>
            <fullName evidence="11 12 13">Sodium/hydrogen exchanger 9B2 isoform X1</fullName>
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    <gene>
        <name evidence="9" type="primary">Slc9b2</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Mixed</tissue>
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    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10">SDA103</strain>
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    <reference evidence="11 12 13" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
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                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
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        <property type="molecule type" value="mRNA"/>
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        <property type="component" value="Chromosome 29"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cation/H_exchanger_TM"/>
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    <dbReference type="InterPro" id="IPR051843">
        <property type="entry name" value="CPA1_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31102">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31102:SF23">
        <property type="entry name" value="SI:DKEY-162B23.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="234"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="513"/>
        </location>
    </feature>
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        <location>
            <begin position="134"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AHH37960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053533574.1"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_053533575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_053533576.1"/>
        </source>
    </evidence>
    <sequence length="546" mass="57992" checksum="F30D6F8676A641F5" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5UA09</accession>
    <name>W5UA09_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein 178A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 13" type="primary">tmem178a</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM178 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT409456">
        <property type="protein sequence ID" value="AHH38685.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017314585.1">
        <property type="nucleotide sequence ID" value="XM_017459096.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UA09"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000004275"/>
    <dbReference type="GeneID" id="108259519"/>
    <dbReference type="KEGG" id="ipu:108259519"/>
    <dbReference type="CTD" id="130733"/>
    <dbReference type="GeneTree" id="ENSGT00390000015299"/>
    <dbReference type="OMA" id="QYGWSIC"/>
    <dbReference type="OrthoDB" id="9941453at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045671">
        <property type="term" value="P:negative regulation of osteoclast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039625">
        <property type="entry name" value="T178A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32005:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 178A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32005">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 178B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13903">
        <property type="entry name" value="Claudin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AHH38685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017314585.1"/>
        </source>
    </evidence>
    <sequence length="306" mass="33903" checksum="5D6384798AFBE527" modified="2014-04-16" version="1">MEKRSLVTAASFCMSVLALVLLVTAIFTDHWYETDTRRHKRNCDRYGAESNDQKNREMPIYHLPLVDSTGSGGGGGTGGSRRNSVALMKPIHVGSREEELLENWRAILGMGILETECGRPLFPTHSGLWRKCYFRGVDPDIDRLISKGIADRCTAVKYHFSQPIRLRNIPLNLTRTIQQDEWHLLHLRRITAGFMGMAAAVLLCGSVVSAVGFFWEEKLTQHVSGLLFLMSGIFCTISLCTYASSVTYDLSRNPPFIYGLPGDVDHGFGWSVLCGWACLGLTAACGCISTASPFITRHKNTHTSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5UC60</accession>
    <name>W5UC60_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Neuropeptide Y receptor Y8b</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="18">Neuropeptide Y receptor type 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">Npy1r</name>
        <name evidence="20" type="synonym">npy8br</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT411802">
        <property type="protein sequence ID" value="AHH39611.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017322552.1">
        <property type="nucleotide sequence ID" value="XM_017467063.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UC60"/>
    <dbReference type="SMR" id="W5UC60"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000017108"/>
    <dbReference type="GeneID" id="108265010"/>
    <dbReference type="KEGG" id="ipu:108265010"/>
    <dbReference type="CTD" id="30713"/>
    <dbReference type="GeneTree" id="ENSGT00940000164226"/>
    <dbReference type="OMA" id="HEAIPSC"/>
    <dbReference type="OrthoDB" id="9046662at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042923">
        <property type="term" value="F:neuropeptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004983">
        <property type="term" value="F:neuropeptide Y receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15397">
        <property type="entry name" value="7tmA_NPY4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000062">
        <property type="entry name" value="Neuropeptide Y receptor type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000351">
        <property type="entry name" value="NPY1_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000611">
        <property type="entry name" value="NPY_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24235">
        <property type="entry name" value="NEUROPEPTIDE Y RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24235:SF25">
        <property type="entry name" value="NEUROPEPTIDE Y RECEPTOR TYPE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01013">
        <property type="entry name" value="NRPEPTIDEY1R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01012">
        <property type="entry name" value="NRPEPTIDEYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 15" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11 15 18" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 15" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="97"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="147"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="202"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="293"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="48"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AHH39611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017322552.1"/>
        </source>
    </evidence>
    <sequence length="377" mass="42529" checksum="03C0845CC3C0EE5A" modified="2014-04-16" version="1">MDEQLVMEGAHNITQPWLGWEEVCSLSLGGTTFLIMAYSTMLAVGLVGNTCVVLVILRQKEMHNVTNIFIANLSCSDILVCLICLPSTITYTLMDRWILGVALCKLTSFVQCLSITVSIYTLVLITLERHQLIIHPTGWKPVLGHSYLAVAVTWIMGCFVSLPFFSFTLLVDFKSQNLSIPMNPSDSHLVCMEKWPSEGNRLAYTTSLLVFQYCLPLILISICYLRIFLRLSHRKDIIERTRDARQRNAKHFKRINAMLASIVALFALCWLPLYVFNAIFDWNHEAITWCHDAVFSVCHLTAMASTCVNPIIYGFLNNNFQKELKSLLYQCRCSGVPQNYESFPLSTVSTDVTKGSILSNGSDTIAVSPQQQEKECL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5UCE9</accession>
    <name>W5UCE9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transitional endoplasmic reticulum ATPase</fullName>
            <ecNumber evidence="5">3.6.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Valosin-containing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25 27" type="primary">vcp</name>
    </gene>
    <organism evidence="25">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="25">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">SDA103</strain>
        </source>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="27">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.6" evidence="5"/>
    <dbReference type="EMBL" id="JT412018">
        <property type="protein sequence ID" value="AHH39696.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017307754.1">
        <property type="nucleotide sequence ID" value="XM_017452265.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UCE9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000011536"/>
    <dbReference type="GeneID" id="100304825"/>
    <dbReference type="KEGG" id="ipu:100304825"/>
    <dbReference type="CTD" id="7415"/>
    <dbReference type="GeneTree" id="ENSGT00900000141071"/>
    <dbReference type="OMA" id="VWPAYPE"/>
    <dbReference type="OrthoDB" id="27435at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19519">
        <property type="entry name" value="RecA-like_CDC48_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19528">
        <property type="entry name" value="RecA-like_CDC48_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000004">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.10:FF:000001">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000003">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000048">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005938">
        <property type="entry name" value="AAA_ATPase_CDC48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01243">
        <property type="entry name" value="CDC48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF69">
        <property type="entry name" value="TRANSITIONAL ENDOPLASMIC RETICULUM ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0072">Autophagy</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="17" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="24">
        <location>
            <begin position="25"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="23">
        <location>
            <begin position="125"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="22">
        <location>
            <begin position="237"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="22">
        <location>
            <begin position="510"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="708"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="768"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="768"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="21">
        <location>
            <begin position="778"/>
            <end position="793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00031860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="AHH39696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_017307754.1"/>
        </source>
    </evidence>
    <sequence length="806" mass="89327" checksum="F6619880A6FF14F2" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5UD65</accession>
    <name>W5UD65_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Claudin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CLDN22</name>
        <name evidence="11" type="synonym">cldn33b</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell junction</location>
            <location evidence="8">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="EMBL" id="JT412704">
        <property type="protein sequence ID" value="AHH39961.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017335197.1">
        <property type="nucleotide sequence ID" value="XM_017479708.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UD65"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000011756"/>
    <dbReference type="GeneID" id="108271850"/>
    <dbReference type="KEGG" id="ipu:108271850"/>
    <dbReference type="CTD" id="751666"/>
    <dbReference type="GeneTree" id="ENSGT00940000164897"/>
    <dbReference type="OMA" id="SYMAHLA"/>
    <dbReference type="OrthoDB" id="8612291at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0796">Tight junction</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU060637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AHH39961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017335197.1"/>
        </source>
    </evidence>
    <sequence length="222" mass="24201" checksum="74DF6C51F650F8FB" modified="2014-04-16" version="1">MSNPCSAILELLGMAVGVGAWLCSLGATIMPNWLTLSTELLVVESYEVGLWESCVVQEGAVTECRAFESLLGLSLNLTLARICMCISDAIGILGLLIAVPGLRLVKSCGGSQGWRVKRGIKITAGVMGLTAGILELFPVSNIAHETVLKFHDHTVPHTVPRWEFGDALFIGWAAGFFHIISAVLFFVSCCGSEENEMHIEYHHHPEKFQPENSSSKKRVEYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>W5UET8</accession>
    <name>W5UET8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Noggin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13 15" type="primary">nog3</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the noggin family.</text>
    </comment>
    <dbReference type="EMBL" id="JT413016">
        <property type="protein sequence ID" value="AHH40084.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017338158.1">
        <property type="nucleotide sequence ID" value="XM_017482669.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UET8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018088"/>
    <dbReference type="GeneID" id="108273449"/>
    <dbReference type="KEGG" id="ipu:108273449"/>
    <dbReference type="CTD" id="30173"/>
    <dbReference type="GeneTree" id="ENSGT00390000006009"/>
    <dbReference type="OMA" id="LWSHTFC"/>
    <dbReference type="OrthoDB" id="5950649at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009953">
        <property type="term" value="P:dorsal/ventral pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045596">
        <property type="term" value="P:negative regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001649">
        <property type="term" value="P:osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.520">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008717">
        <property type="entry name" value="Noggin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10494">
        <property type="entry name" value="BONE MORPHOGENETIC PROTEIN INHIBITOR, NOGGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10494:SF5">
        <property type="entry name" value="NOGGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05806">
        <property type="entry name" value="Noggin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008129">
        <property type="entry name" value="Noggin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 9" id="KW-0891">Chondrogenesis</keyword>
    <keyword evidence="3 9" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 9" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="136"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="11">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="146"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="169"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="175"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="198"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR008129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR008129-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR008129-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AHH40084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017338158.1"/>
        </source>
    </evidence>
    <sequence length="225" mass="26023" checksum="E183BEEB26A469E1" modified="2014-04-16" version="1">MDNMDNVSHFLAMCVLFFALGFRIEGVSGQHHTPMHYLVRPVPSDSLPVVLIKEDPDPVLDPKERDLNETELRSILGAHFDARFMSVMLPEGDSGMDSEGTRQQFLQRPMPKEIRNLDFDTAHDAKKQNKKLRRRLQLFIWAYAFCPVVYAWQDLGSRFWPRYVKMGSCLAKRSCSMPEGMMCKPSKSVHFTLLRWHCTSRRPSKCTWIPVHYPVISECKCSCPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5UFL4</accession>
    <name>W5UFL4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Nuclear inhibitor of protein phosphatase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Protein phosphatase 1 regulatory inhibitor subunit 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">Ppp1r8</name>
        <name evidence="18" type="synonym">ppp1r8b</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT414841">
        <property type="protein sequence ID" value="AHH40776.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017330735.1">
        <property type="nucleotide sequence ID" value="XM_017475246.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UFL4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021729"/>
    <dbReference type="GeneID" id="108269396"/>
    <dbReference type="KEGG" id="ipu:108269396"/>
    <dbReference type="CTD" id="799896"/>
    <dbReference type="OMA" id="RMHGYSL"/>
    <dbReference type="OrthoDB" id="4096268at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22674">
        <property type="entry name" value="FHA_PPP1R8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000012">
        <property type="entry name" value="Nuclear inhibitor of protein phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050923">
        <property type="entry name" value="Cell_Proc_Reg/RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23308">
        <property type="entry name" value="NUCLEAR INHIBITOR OF PROTEIN PHOSPHATASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FHA" evidence="15">
        <location>
            <begin position="48"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="286"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AHH40776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017330735.1"/>
        </source>
    </evidence>
    <sequence length="342" mass="37699" checksum="F21276C9E3A7D8B1" modified="2014-04-16" version="1">MMAKNTLANIPNFDCPSWAGKPPPGLHLDVVKGDKLIEKLIIDEKKFYLFGRNPDLCDFTIDHQSCSRVHAALVYHKHLKRLFLIDLNSTHGTFLGHIRLEPHKPQQVPIDSTMSFGASTRVYTIREKPQSQANVSAGDKTGDDEELKGLLGLPEEETELENLTEFNTAHNKRISTLTIEEGNLDIQRPKRRRKSSRVSFTEDEEIINPEDVDPSVGRFRNMVQTAVVPLKKKKMESGNVLGIEDTVTHHAHSFPLKGGLYGDLPPAGHEAHAAGGTMLGGLPLPNPAPEVDLAPEAPLPPITLNPTPVSGPYAPEGLSEPRKKKYAKEAWPGKKPTPSLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5UGS3</accession>
    <name>W5UGS3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial peptide methionine sulfoxide reductase</fullName>
            <ecNumber evidence="2">1.8.4.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Peptide-methionine (S)-S-oxide reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Protein-methionine-S-oxide reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MSRA</name>
        <name evidence="13" type="synonym">msrab</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(S)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:14217"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12315"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:44120"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[thioredoxin]-disulfide + L-methionine + H2O = L-methionine (S)-S-oxide + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:19993"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58772"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.11" evidence="2"/>
    <dbReference type="EMBL" id="JT409883">
        <property type="protein sequence ID" value="AHH38862.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JT419258">
        <property type="protein sequence ID" value="AHH43007.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017332406.1">
        <property type="nucleotide sequence ID" value="XM_017476917.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UGS3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000016454"/>
    <dbReference type="GeneID" id="108270340"/>
    <dbReference type="KEGG" id="ipu:108270340"/>
    <dbReference type="CTD" id="503993"/>
    <dbReference type="OMA" id="QYRSIAF"/>
    <dbReference type="OrthoDB" id="77405at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1060.10:FF:000001">
        <property type="entry name" value="Peptide methionine sulfoxide reductase MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1060.10">
        <property type="entry name" value="Peptide methionine sulphoxide reductase MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01401">
        <property type="entry name" value="MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036509">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050162">
        <property type="entry name" value="MsrA_MetSO_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42799">
        <property type="entry name" value="MITOCHONDRIAL PEPTIDE METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42799:SF22">
        <property type="entry name" value="PEPTIDE-METHIONINE (S)-S-OXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55068">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptide methionine sulphoxide reductase MsrA" evidence="10">
        <location>
            <begin position="72"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AHH38862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017332406.1"/>
        </source>
    </evidence>
    <sequence length="242" mass="27188" checksum="F5EEEA7F5523F563" modified="2014-04-16" version="1">MFVYLGKRANKCLSTIFILRISLANLSMASKSVLPTKDRALKGRETKMVVAAKHAVTGKPTVEPFPDGTEMIMFGMGCFWGAERRFWTLPGVFSTQVGYAGGFTPNPTYKEVCSGLTGHTEVVRVVYPPKDVSLEKLLKVFWESHDPTQGMAQGNDKGTQYRSAIYTYSPEQQEQAMRTKETYQQALKQKGKGEITTEIRQDEVFYYAENYHQQYLHKVPNGYCGLKGTGVTCTFSSNKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>W5UHU5</accession>
    <name>W5UHU5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12 14">Paired mesoderm homeobox protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">Prrx1</name>
        <name evidence="14" type="synonym">prrx1a</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="JT415470">
        <property type="protein sequence ID" value="AHH41709.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017347109.1">
        <property type="nucleotide sequence ID" value="XM_017491620.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UHU5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000024024"/>
    <dbReference type="GeneID" id="108278326"/>
    <dbReference type="KEGG" id="ipu:108278326"/>
    <dbReference type="CTD" id="406431"/>
    <dbReference type="GeneTree" id="ENSGT00940000159466"/>
    <dbReference type="OMA" id="CTRTASP"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086014">
        <property type="term" value="P:atrial cardiac muscle cell action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904888">
        <property type="term" value="P:cranial skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001837">
        <property type="term" value="P:epithelial to mesenchymal transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001947">
        <property type="term" value="P:heart looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000066">
        <property type="entry name" value="Paired mesoderm homeobox protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003654">
        <property type="entry name" value="OAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043378">
        <property type="entry name" value="PRRX1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46385:SF1">
        <property type="entry name" value="PAIRED MESODERM HOMEOBOX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46385">
        <property type="entry name" value="PAIRED MESODERM HOMEOBOX PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03826">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50803">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="83"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="OAR" evidence="11">
        <location>
            <begin position="213"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="85"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="38"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="60"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AHH41709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017347109.1"/>
        </source>
    </evidence>
    <sequence length="234" mass="26135" checksum="BF3B21706EEE5A6F" modified="2014-04-16" version="1">MTSGYAAHVMDRAINLDHLHAKKNFSVSHLLDLEDAREMAGAQAEESSAEAGTGSLESPGLTSGSDTAQQDNEQLNSEEKKKRKQRRNRTTFNSSQLQALERVFERTHYPDAFVREDLARRVNLTEARVQVWFQNRRAKFRRNERALLASKNASLLKTYSGDVTSVEQPMVPRPAPRPSDYLSWGNSPPYSAMASYSPSCANNNTAQGVNMANSIANLRLKAKEYSMNQVPTVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5UHW6</accession>
    <name>W5UHW6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5 7">Transmembrane gamma-carboxyglutamic acid protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">Prrg2</name>
        <name evidence="7" type="synonym">prrg2</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT409089">
        <property type="protein sequence ID" value="AHH38558.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="AHH41739.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017351120.1">
        <property type="nucleotide sequence ID" value="XM_017495631.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UHW6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009833"/>
    <dbReference type="GeneID" id="108280539"/>
    <dbReference type="KEGG" id="ipu:108280539"/>
    <dbReference type="CTD" id="5639"/>
    <dbReference type="OMA" id="IYCYKAK"/>
    <dbReference type="OrthoDB" id="9945709at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="4.10.740.10">
        <property type="entry name" value="Coagulation Factor IX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017857">
        <property type="entry name" value="Coagulation_fac-like_Gla_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035972">
        <property type="entry name" value="GLA-like_dom_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000294">
        <property type="entry name" value="GLA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050442">
        <property type="entry name" value="Peptidase_S1_coag_factors"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278">
        <property type="entry name" value="COAGULATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278:SF38">
        <property type="entry name" value="TRANSMEMBRANE GAMMA-CARBOXYGLUTAMIC ACID PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00594">
        <property type="entry name" value="Gla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00001">
        <property type="entry name" value="GLABLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00069">
        <property type="entry name" value="GLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57630">
        <property type="entry name" value="GLA-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00011">
        <property type="entry name" value="GLA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50998">
        <property type="entry name" value="GLA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="120"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Gla" evidence="4">
        <location>
            <begin position="64"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="186"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AHH41739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017351120.1"/>
        </source>
    </evidence>
    <sequence length="218" mass="24100" checksum="2DF1ADE4171E5817" modified="2014-04-16" version="1">MCTVLILCGTDKMVGFTQLCLGLMSVFHLVLTRGINNNNNNNNNRNVFLHETSADSFLSRSLLYNSWDLELVTPGDLERECREEVCSYEEAREVFEDDRKTAAFWKTYTNSHVSSPRVDVAGLTAGILGTVVFVIIVLVLSCYCYKRKRKTVRRSGSVPVRLAADGRPAPETVPLANIAAPGLPSYNEALNRSGQYDAPPPPYSGEEPSAPTEPQPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5UJL7</accession>
    <name>W5UJL7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutaredoxin-related protein 5, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Monothiol glutaredoxin-5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 11 12 13" type="primary">glrx5</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11 12 13" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11 12 13">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT416973">
        <property type="protein sequence ID" value="AHH42216.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017311750.1">
        <property type="nucleotide sequence ID" value="XM_017456261.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053531864.1">
        <property type="nucleotide sequence ID" value="XM_053675889.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053531865.1">
        <property type="nucleotide sequence ID" value="XM_053675890.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UJL7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000023198"/>
    <dbReference type="GeneID" id="108258029"/>
    <dbReference type="KEGG" id="ipu:108258029"/>
    <dbReference type="CTD" id="51218"/>
    <dbReference type="OMA" id="YEVLDEP"/>
    <dbReference type="OrthoDB" id="415696at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03028">
        <property type="entry name" value="GRX_PICOT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000005">
        <property type="entry name" value="Glutaredoxin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033658">
        <property type="entry name" value="GRX_PICOT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004480">
        <property type="entry name" value="Monothiol_GRX-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00365">
        <property type="entry name" value="Grx4 family monothiol glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293">
        <property type="entry name" value="GLUTAREDOXIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293:SF16">
        <property type="entry name" value="GLUTAREDOXIN-RELATED PROTEIN 5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="8">
        <location>
            <begin position="57"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00076083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AHH42216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017311750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053531864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_053531865.1"/>
        </source>
    </evidence>
    <sequence length="158" mass="17542" checksum="7243322DF29A33B8" modified="2014-04-16" version="1">MNNMLNSVLRQTARSVRGISLTQRHSGRLGAAGARWMCSSSSEVLKNVEQIVKKDKVVVFMKGTPAQPMCGFSNAVVQILRMHGVEKYTAYNVLENQELRQGVKTFSNWPTIPQVFFNGEFVGGCDILLQMHQSGDLVEELDKLGIRSALLDTQTPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5ULU0</accession>
    <name>W5ULU0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10 12 13">Galactosylceramide sulfotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">GAL3ST1</name>
        <name evidence="12 13" type="synonym">gal3st1a</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12 13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the galactose-3-O-sulfotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="JT414573">
        <property type="protein sequence ID" value="AHH40672.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017307613.1">
        <property type="nucleotide sequence ID" value="XM_017452124.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017307614.1">
        <property type="nucleotide sequence ID" value="XM_017452125.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ULU0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030017"/>
    <dbReference type="GeneID" id="108255842"/>
    <dbReference type="KEGG" id="ipu:108255842"/>
    <dbReference type="CTD" id="793735"/>
    <dbReference type="OMA" id="CAPKVDI"/>
    <dbReference type="OrthoDB" id="514299at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001733">
        <property type="term" value="F:galactosylceramide sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006682">
        <property type="term" value="P:galactosylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042552">
        <property type="term" value="P:myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000807">
        <property type="entry name" value="galactosylceramide sulfotransferase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009729">
        <property type="entry name" value="Gal-3-0_sulfotransfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14647">
        <property type="entry name" value="GALACTOSE-3-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14647:SF56">
        <property type="entry name" value="GALACTOSYLCERAMIDE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06990">
        <property type="entry name" value="Gal-3-0_sulfotr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AHH40672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017307613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017307614.1"/>
        </source>
    </evidence>
    <sequence length="431" mass="49974" checksum="8D88CEEE6746E467" modified="2014-04-16" version="1">MMVVKQRKQGKQWRSVCKGLVLGALLTSCMILLYCLSAPEVQFRLPEIPVPYSCAHHPSLGQSPAASSSIHRGLRRERPCTPKVDIMFMKTHKTASSTILNILFRFGEKHHLKFAFPSSRNDFFYPSLFYRTQVKDYGSGMCFNIMCNHMRFNEAEVAKVLPADTTYITILRDPAELFESSFHYFGRLVPFTWKISGGDKMEEFLSQPELYYDPEGFNSFYLKNLLFFDFGQDNNLPLADPRVEEGVRAISKRFHLVMLVEHFEESLILLKDALCWDMDDLLFFKLNVRKGSTVSKLTPELRTKALQWNAIDWKLYQHFNTTFWKKVEAYGRERMANDVAELRRRNSQMAAICIEGGHAVDAGSIHETSMQPWQPIGEKSIMGYNLKKNVDKAHQKLCRKMLTPELQYLTDLGVNLWITKLWGHVRDILDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>W5UM01</accession>
    <name>W5UM01_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">AKT2</name>
        <name evidence="18 19" type="synonym">akt2</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18 19" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18 19">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JT419164">
        <property type="protein sequence ID" value="AHH42976.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534034.1">
        <property type="nucleotide sequence ID" value="XM_053678059.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534035.1">
        <property type="nucleotide sequence ID" value="XM_053678060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UM01"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000001435"/>
    <dbReference type="GeneID" id="108264340"/>
    <dbReference type="KEGG" id="ipu:108264340"/>
    <dbReference type="OMA" id="DRCECLG"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01241">
        <property type="entry name" value="PH_PKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05595">
        <property type="entry name" value="STKc_PKB_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000033">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000027">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000838">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034677">
        <property type="entry name" value="Akt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039026">
        <property type="entry name" value="PH_PKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 16 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PH" evidence="13">
        <location>
            <begin position="5"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="147"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="15">
        <location>
            <begin position="405"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="447"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="466"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="RuleBase" id="RU000304"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50003"/>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AHH42976.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_053534034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_053534035.1"/>
        </source>
    </evidence>
    <sequence length="476" mass="55117" checksum="C12FD47F68E0F2EB" modified="2014-04-16" version="1">MNEVSVVREGWLHKRGEYIKTWRPRYFILKSDGSFIGYKEKPDVSEPNLPPLNNFSVAECQLMKTERPRPNTFVIRCLQWTTVIERTFHVDSNAEREEWIRAIQAVANGLKTREEEEPMDINFSSTGDSSMDTAMAKSRNKVTMSDFDYLKLLGKGTFGKVILVREKATGVHYAMKILRKEVIIAKDEVAHTVTESRVLQNTRHPFLTTLKYAFQTHDRLCFVMEYANGGELFFHLSRERVFTEDRAQFYGAEIVSALEYLHSRDVVYRDLKLENLMLDKDGHIKITDFGLCKEGITNEATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHERLFELILMEEIRFPRNLSPDAKSLLAGLLKKDPKQRLGGSIEDAKEVMAHKFFSSINWQDVLQKKLTPPFKPQVTSETDTRYFDEEFTLQTITVTPPDQYDSLDAEDSDARTHFPQFSYSASVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2015-05-27" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A0D5CDW0</accession>
    <name>A0A0D5CDW0_ICTPU</name>
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        <submittedName>
            <fullName evidence="11">SLAM family member 7 precursor</fullName>
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        <submittedName>
            <fullName evidence="9">T cell surface antigen CD2</fullName>
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    <gene>
        <name evidence="11" type="primary">slamf7</name>
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    <organism evidence="9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="9 11" key="2">
        <citation type="journal article" date="2015" name="Mol. Immunol." volume="66" first="126" last="138">
            <title>The Src tyrosine kinase Lck binds to CD2, CD4-1, and CD4-2 T cell co-receptors in channel catfish, Ictalurus punctatus.</title>
            <authorList>
                <person name="Taylor E.B."/>
                <person name="Wilson M."/>
                <person name="Bengten E."/>
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            <dbReference type="PubMed" id="25771179"/>
            <dbReference type="DOI" id="10.1016/j.molimm.2015.02.023"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">TS32.15</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="KEGG" id="ipu:108274237"/>
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    <dbReference type="OrthoDB" id="8963224at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="AgBase"/>
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        <property type="entry name" value="Ig-like_dom"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
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    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7 11" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035982272" evidence="7 11">
        <location>
            <begin position="24"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="118"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="238"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="251"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AJW77402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="NP_001316255.1"/>
        </source>
    </evidence>
    <sequence length="333" mass="37418" checksum="101FA5B1307C01C4" modified="2015-05-27" version="1">MNTKGSVIVLFLCFLTLFTLTDCSTCQKNILEGGSLTFKLTDRPLKDNDRFTIKKDNKVVVLKKNNKQEGPGIVKKDSLELLHVKLSDSGTYGVEVFDVNGTNVKSYTEIVCVYAKVPKPRVTITCQDEKVDFRCDVEVDKDQRSAIFYSWHKNGKELKKNVFNFSINAEEDKSKYTCTAQNPVHNSTSDPVEAACFKRTLFGFDFWFMVGLLAGGGGLVLLLIIVLVTLACRSCRRKDKQQRDEEESRLNYVNASQSSGQQKSKHTARGQPAPPEPYDDVSAQDVPCQAEPKPRSQQRGRPPPPPIDDEEEHPPPLPQPRKKAQVKKREAPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PIF6</accession>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">phf13</name>
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    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Modulates chromatin structure and DNA damage response by regulating key determinants of chromatin compaction and DNA damage response. Binds H3K4me3-containing chromatin and promotes DNA condensation by recruiting corepressors such as TRIM28 and H3K9 methyltransferase SETDB1. Required for normal chromosome condensation during the early stages of mitosis. Required for normal chromosome separation during mitosis. Increases both chromatin-associated levels and activity of H3K9 methyltransferases, such as SETDB1, thus enhancing H3K9 trimethylation. Essential for testicular stem-cell differentiation and sustained spermatogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
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    <dbReference type="AlphaFoldDB" id="A0A2D0PIF6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005153"/>
    <dbReference type="GeneID" id="108255120"/>
    <dbReference type="KEGG" id="ipu:108255120"/>
    <dbReference type="CTD" id="148479"/>
    <dbReference type="OMA" id="IPLHPQC"/>
    <dbReference type="OrthoDB" id="79252at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15632">
        <property type="entry name" value="PHD_PHF13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000039">
        <property type="entry name" value="PHD finger protein 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041947">
        <property type="entry name" value="PHD_PHF13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14571">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SET-26-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14571:SF13">
        <property type="entry name" value="PHD FINGER PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="8" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger PHD-type" evidence="14">
        <location>
            <begin position="278"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="48"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="171"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="177"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017306298.1"/>
        </source>
    </evidence>
    <sequence length="344" mass="38387" checksum="0BFC3771A16A7727" modified="2017-12-20" version="1">MDNSDVKTAAFDSQGYSPTCKRKRTVEDFNKFCTFVLAYAGYIPYPQEEAPLRSSSSPPNSTGSTADSDGLPSPDQPPHKSRKSFSGFKRPANDEPRLHKQPKSTDFLLEGCKKTDKMKKKKKKLTENVKSDESGMFPPFPPGTRVELSAFGRTVKEEPEDYISISTPPSYTEHCAHPSSSSPHRPSSHGTVGCKKEPEAERACWDSPASLGMESRAESREKLALPDGEAVVMKMEGCPGTRTVIRQGKQVVFRDEDGSADDEDIMVDSDDDSWDLVTCFCMKPFAGRAMIECNQCNTWIHLSCAKIRKSNVPETYTCQQCKDSKFDIRRSNRSRMGSRKHLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PL26</accession>
    <name>A0A2D0PL26_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="17 18">Chemerin-like receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17 18" type="primary">LOC108255316</name>
    </gene>
    <organism evidence="16 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17 18" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17 18">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the chemokine-like receptor (CMKLR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017306693.1">
        <property type="nucleotide sequence ID" value="XM_017451204.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017306694.1">
        <property type="nucleotide sequence ID" value="XM_017451205.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PL26"/>
    <dbReference type="GeneID" id="108255316"/>
    <dbReference type="KEGG" id="ipu:108255316"/>
    <dbReference type="OrthoDB" id="6117944at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004875">
        <property type="term" value="F:complement receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14974">
        <property type="entry name" value="7tmA_Anaphylatoxin_R-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000034">
        <property type="entry name" value="G-protein coupled receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000826">
        <property type="entry name" value="Formyl_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24225">
        <property type="entry name" value="CHEMOTACTIC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24225:SF0">
        <property type="entry name" value="N-FORMYL PEPTIDE RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00526">
        <property type="entry name" value="FMETLEUPHER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="107"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="200"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="58"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017306693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017306694.1"/>
        </source>
    </evidence>
    <sequence length="343" mass="38529" checksum="9DA0299E92029502" modified="2017-12-20" version="1">MDTDEAGDYPEYNDHDNKMDSSSAALVPSTVAPAHCTDAICVLLAIANVIIMALGIAGNGLVIWIAGFKIKKTVNTVWYFSLAVSDFLFCAFLPFSIAYIVKREWDFGLFMCKFTSFILFLNMFSSIFLLLIISVDRCVAVMCPVWAQNKRTIRRALVMVALAWTISALLSLPPAIFRDITISNSKHVCYYNYSHDEEHIAVVVCRFIFGFVIPLLIIIICYVLITLKLKNNQTAKSNKPFKIMTALIAVFFICWMPYHIFNLLELNPKYAYILPIGQKFGSTLASANSFMNPLLYAFMGKDFKRKCYALLSKIESTFEEEARSTLRGTSITNSGDGKLSSTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PL58</accession>
    <name>A0A2D0PL58_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Homer protein homolog 1b isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">homer1b</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Postsynaptic density</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Homer family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017306767.1">
        <property type="nucleotide sequence ID" value="XM_017451278.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PL58"/>
    <dbReference type="GeneID" id="108255354"/>
    <dbReference type="KEGG" id="ipu:108255354"/>
    <dbReference type="CTD" id="436769"/>
    <dbReference type="OrthoDB" id="9983798at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0035256">
        <property type="term" value="F:G protein-coupled glutamate receptor binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:G protein-coupled glutamate receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="CDD" id="cd01206">
        <property type="entry name" value="EVH1_Homer_Vesl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000014">
        <property type="entry name" value="Homer homolog 1 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045027">
        <property type="entry name" value="Homer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044100">
        <property type="entry name" value="Homer_EVH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000697">
        <property type="entry name" value="WH1/EVH1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10918">
        <property type="entry name" value="HOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00568">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00461">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50229">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="WH1" evidence="9">
        <location>
            <begin position="36"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="158"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="224"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="198"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="210"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017306767.1"/>
        </source>
    </evidence>
    <sequence length="398" mass="45083" checksum="4ABAA9A82AD63B86" modified="2017-12-20" version="1">MEAREMVLRLPGRASAPSGFGQTTVLRHKEVHHFFFVPFREQPIYSTRAHVFQIDPNTKKNWMSTSKHAVTVSYFYDSTRNVYRIISLDGSKAIINSTITPNMSFTKTSQKFGQWADSRANTVYGLGFSSEHHLSKFADKFAEYKEAARLAKEKSLEKMELASSPSQESPADIHSPLTPPVTSDLCRVNGTGDDVTLSDASPNSELQSDLSPTTPTLAHSPTTTKHWEAELAALKSNNAKLTAALLESTANVKQWKQQLAAYQEEAERLHKRVSELESQSSQANAIKCQKTELNKTIEELEATLKTKEEEMDRLREEVEKAKQLQSQKEMLEQKLEATDARNKDLEEQLADVEQRLETSQLEQENFRKSLKTLLEILDTKIFELTEMRDNLAKLIEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PP20</accession>
    <name>A0A2D0PP20_ICTPU</name>
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        <submittedName>
            <fullName evidence="9">Endosomal transmembrane epsin interactor 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">fam189a2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ENTREP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017307747.1">
        <property type="nucleotide sequence ID" value="XM_017452258.3"/>
    </dbReference>
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    <dbReference type="GeneID" id="108255902"/>
    <dbReference type="KEGG" id="ipu:108255902"/>
    <dbReference type="CTD" id="503960"/>
    <dbReference type="OrthoDB" id="9945596at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007237">
        <property type="entry name" value="CD20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030431">
        <property type="entry name" value="ENTREP1-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17615:SF8">
        <property type="entry name" value="ENDOSOMAL TRANSMEMBRANE EPSIN INTERACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17615">
        <property type="entry name" value="PROTEIN FAM189A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04103">
        <property type="entry name" value="CD20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="341"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="403"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="352"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="422"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017307747.1"/>
        </source>
    </evidence>
    <sequence length="588" mass="64451" checksum="2FC91B5E4C384557" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PP76</accession>
    <name>A0A2D0PP76_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Heat shock protein beta-8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">hspb8</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6 7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017307814.1">
        <property type="nucleotide sequence ID" value="XM_017452325.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017307815.1">
        <property type="nucleotide sequence ID" value="XM_017452326.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PP76"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026811"/>
    <dbReference type="GeneID" id="108255940"/>
    <dbReference type="KEGG" id="ipu:108255940"/>
    <dbReference type="CTD" id="26353"/>
    <dbReference type="GeneTree" id="ENSGT00940000160605"/>
    <dbReference type="OMA" id="PCHYPSR"/>
    <dbReference type="OrthoDB" id="10060792at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101031">
        <property type="term" value="C:protein folding chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034620">
        <property type="term" value="P:cellular response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001436">
        <property type="entry name" value="Alpha-crystallin/sHSP_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043254">
        <property type="entry name" value="HSPB8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46906">
        <property type="entry name" value="HEAT SHOCK PROTEIN BETA-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46906:SF1">
        <property type="entry name" value="HEAT SHOCK PROTEIN BETA-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00299">
        <property type="entry name" value="ACRYSTALLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="4">
        <location>
            <begin position="72"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="57"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017307814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017307815.1"/>
        </source>
    </evidence>
    <sequence length="189" mass="21234" checksum="0D7D4FF3E3EC0D98" modified="2017-12-20" version="1">MAEGDFYPLGSRQRIARDPFSTHFMDPDFSVPMSAFPEDLSLDWPGWARPRLSARLGSPWTGSLRSSFPRTSMSAQSQRGAPPVDPDEPWKVCVNVHSYRPEELSVKTKDNFVEVAGKHEEKQDEGGIVTKNFTKKIQIPVDVDPLTVFASLSPEGVLIIEARQTPPYYLYSNEAPAEYMEEQQEPTTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PQA4</accession>
    <name>A0A2D0PQA4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mitochondrial-processing peptidase subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Alpha-MPP</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Inactive zinc metalloprotease alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">pmpca</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Substrate recognition and binding subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer of PMPCA (alpha) and PMPCB (beta) subunits, forming the mitochondrial processing protease (MPP) in which PMPCA is involved in substrate recognition and binding and PMPCB is the catalytic subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017307265.1">
        <property type="nucleotide sequence ID" value="XM_017451776.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PQA4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020760"/>
    <dbReference type="GeneID" id="108255664"/>
    <dbReference type="KEGG" id="ipu:108255664"/>
    <dbReference type="CTD" id="23203"/>
    <dbReference type="OrthoDB" id="277191at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000010">
        <property type="entry name" value="Mitochondrial-processing peptidase alpha subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000014">
        <property type="entry name" value="Mitochondrial-processing peptidase alpha subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF49">
        <property type="entry name" value="MITOCHONDRIAL-PROCESSING PEPTIDASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="14">
        <location>
            <begin position="69"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="15">
        <location>
            <begin position="224"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017307265.1"/>
        </source>
    </evidence>
    <sequence length="517" mass="57704" checksum="24294ACB0B8638C7" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PRG6</accession>
    <name>A0A2D0PRG6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">arfgef1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
            <location evidence="3">trans-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017308614.1">
        <property type="nucleotide sequence ID" value="XM_017453125.3"/>
    </dbReference>
    <dbReference type="GeneID" id="108256356"/>
    <dbReference type="KEGG" id="ipu:108256356"/>
    <dbReference type="CTD" id="10565"/>
    <dbReference type="OrthoDB" id="18431at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000143">
        <property type="entry name" value="ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000003">
        <property type="entry name" value="Brefeldin A-inhibited guanine nucleotide-exchange protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
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        <property type="entry name" value="BREFELDIN A-INHIBITED GUANINE NUCLEOTIDE-EXCHANGE PROTEIN 1"/>
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    </dbReference>
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        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
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        <location>
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            <begin position="230"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
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            <dbReference type="ARBA" id="ARBA00022927"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50190"/>
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            <dbReference type="Proteomes" id="UP000221080"/>
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        <source>
            <dbReference type="RefSeq" id="XP_017308614.1"/>
        </source>
    </evidence>
    <sequence length="1864" mass="209925" checksum="DCF8915F7A6BEF2E" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PX93</accession>
    <name>A0A2D0PX93_ICTPU</name>
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        <submittedName>
            <fullName evidence="14">GS homeobox 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">gsx2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2">Belongs to the Antp homeobox family.</text>
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        <property type="component" value="Chromosome 25"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GS homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042191">
        <property type="entry name" value="GSH1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
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    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47421">
        <property type="entry name" value="GS HOMEOBOX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="130"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="132"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="189"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017311118.1"/>
        </source>
    </evidence>
    <sequence length="234" mass="25607" checksum="46C63D9782D5E876" modified="2017-12-20" version="1">MSRSFYVDSLIIKDSARPAPLGEHTAQDFFIPIGMHAPGVMGVPAAACPSRKSGAFCVCPLCVASHVHSPRAGLPLLKAPGFPSGDAPYCQRLAQQHNTAPVCTPPAYSVTDPRRYHCLSIGASDNGHVQNGKRMRTAFTSTQLLELEREFSSNMYLSRLRRIEIATYLNLSEKQVKIWFQNRRVKHKKEGKGAQRSAHAGCKCSGNHAPYPRSEDEESLSPASATEEKEGSPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q1X4</accession>
    <name>A0A2D0Q1X4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Homeobox protein NANOG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nanog</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017312287.1">
        <property type="nucleotide sequence ID" value="XM_017456798.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q1X4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000001735"/>
    <dbReference type="GeneID" id="108258275"/>
    <dbReference type="KEGG" id="ipu:108258275"/>
    <dbReference type="CTD" id="79923"/>
    <dbReference type="OMA" id="YGLMYPQ"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050460">
        <property type="entry name" value="Distal-less_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24327">
        <property type="entry name" value="HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24327:SF88">
        <property type="entry name" value="NANOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="7">
        <location>
            <begin position="209"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="211"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="108"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="176"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="369"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="133"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="197"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="383"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017312287.1"/>
        </source>
    </evidence>
    <sequence length="399" mass="43739" checksum="3B05A948D45415B0" modified="2017-12-20" version="1">MADWKVPVSYNYSPSYHAYAYGLVYQQTPEQTHANVSWAEAAYGSSVGMAGGYYTSQPPPSSQTPPGSPEYNNPASEGHYPGAVVYYADAKAHTQTGRLFLAHNRVQFDQRSKEQERAGSDTPSDSESHTPDSWSSGSSREGSAAQVDLDHHDWIKKEQAHEPDSGGLDANEVASNSLSAASEGPECLTVERDGGLPAASQEAAPQQQTRKAKARTAFSEEQMSALNDRFNVQRYLTPAEMKTLAGLTGLTYKQVKTWFQNRRMKLKRHQKDSSWVSERYITAGIPNGQTTHAQFQVDAPMLTQDLYNNPQFRDAVFKKSPPQTSFYLNYSLPRSPSQISTGRQGSWRLPPAVQHYEFLNPSSYVPVGGDAGTGDVGTVDPGSSPTQMATAHNATQWSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q2E4</accession>
    <name>A0A2D0Q2E4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription initiation factor TFIID subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">taf5</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat TAF5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017312903.1">
        <property type="nucleotide sequence ID" value="XM_017457414.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q2E4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022470"/>
    <dbReference type="GeneID" id="108258645"/>
    <dbReference type="KEGG" id="ipu:108258645"/>
    <dbReference type="CTD" id="6877"/>
    <dbReference type="OMA" id="HNHPVWD"/>
    <dbReference type="OrthoDB" id="10266330at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08044">
        <property type="entry name" value="TAF5_NTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000243">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.500">
        <property type="entry name" value="TFIID subunit TAF5, NTD2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007582">
        <property type="entry name" value="TFIID_NTD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037264">
        <property type="entry name" value="TFIID_NTD2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF4">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04494">
        <property type="entry name" value="TFIID_NTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160897">
        <property type="entry name" value="Taf5 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="TFIID subunit TAF5 NTD2" evidence="11">
        <location>
            <begin position="147"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="404"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="477"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="519"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="561"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="603"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="645"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="322"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="322"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="345"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017312903.1"/>
        </source>
    </evidence>
    <sequence length="738" mass="81908" checksum="ADB66D4B324E7D50" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q394</accession>
    <name>A0A2D0Q394_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Receptor activity-modifying protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ramp2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RAMP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017312156.1">
        <property type="nucleotide sequence ID" value="XM_017456667.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q394"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018409"/>
    <dbReference type="GeneID" id="108258188"/>
    <dbReference type="KEGG" id="ipu:108258188"/>
    <dbReference type="CTD" id="10266"/>
    <dbReference type="OMA" id="QIFVDIH"/>
    <dbReference type="OrthoDB" id="8652678at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015026">
        <property type="term" value="F:coreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008277">
        <property type="term" value="P:regulation of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.510">
        <property type="entry name" value="Receptor activity modifying family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006985">
        <property type="entry name" value="RAMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038126">
        <property type="entry name" value="RAMP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14076:SF10">
        <property type="entry name" value="RAMP2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14076">
        <property type="entry name" value="RECEPTOR ACTIVITY MODIFYING PROTEIN RAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04901">
        <property type="entry name" value="RAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012722821" evidence="13">
        <location>
            <begin position="33"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="37"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017312156.1"/>
        </source>
    </evidence>
    <sequence length="211" mass="23568" checksum="EA6189BAFA989EBD" modified="2017-12-20" version="1">MSPHVSSPRLRPTWSPSFWVACMVLMFGRLQALVPSEATPPKRSEEQRGLGNTPLFPGVPTTPLTTTSVSTEEKPLSSPCDVYCSLCDEATLPTVQCYSEFLKICRLNLQNELDNVNSTDLCVWDKVKSPYNNFTMCSEGIAECLALPWPNRLVEDMFVEIHSSFFQECPIETLRDPPPNVIFALVMTPICLIPAMVVLVVLKTKNGDRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q3E6</accession>
    <name>A0A2D0Q3E6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Transcription initiation factor IIA subunit 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">gtf2a1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8 9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIA subunit 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017312206.1">
        <property type="nucleotide sequence ID" value="XM_017456717.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017312207.1">
        <property type="nucleotide sequence ID" value="XM_017456718.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q3E6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022184"/>
    <dbReference type="GeneID" id="108258216"/>
    <dbReference type="KEGG" id="ipu:108258216"/>
    <dbReference type="CTD" id="2957"/>
    <dbReference type="GeneTree" id="ENSGT00940000156726"/>
    <dbReference type="OrthoDB" id="6275927at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07976">
        <property type="entry name" value="TFIIA_alpha_beta_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.100:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.18.10:FF:000002">
        <property type="entry name" value="Transcription initiation factor IIA subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.18.10">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004855">
        <property type="entry name" value="TFIIA_asu/bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009088">
        <property type="entry name" value="TFIIA_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12694">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12694:SF7">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03153">
        <property type="entry name" value="TFIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01371">
        <property type="entry name" value="TFIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47396">
        <property type="entry name" value="Transcription factor IIA (TFIIA), alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50784">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="226"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="226"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="275"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017312206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017312207.1"/>
        </source>
    </evidence>
    <sequence length="371" mass="41328" checksum="B4F49D6FA558B3F8" modified="2017-12-20" version="1">MASSANSNPVPKLYKTVMEDVINEVRELFLDEGVDEQVLMELKTLWESKLMQSKAVDGFHSEEQQVLQAPQTQVPQVQQTQQTTQPPQVLLPPAQQAPQQQVIIQEPKMLQHMSATGMSAAATAATLALNTSPYQQIITNQGQILQVVRAANGAQYIIQQQPPIMVQQQVLPQIQTSGVQAPVIQQVLTPLQGGLPQQTGVIIQPQQIVLTQNKVQQNTQVMQAAMAQGQTQTQPQTHTQTPQQPQTAQQQAQPQQQPQQQAPMMLQVDGAGDTSSEEDEDEEDEYDEDEDEDKDKDGGEDGQVEEEPLNSGDDVSDEEDQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYVFSKAIGDAEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q3F8</accession>
    <name>A0A2D0Q3F8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Methyltransferase-like protein 23 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">mettl23</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the methyltransferase superfamily. METTL23 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017311900.1">
        <property type="nucleotide sequence ID" value="XM_017456411.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q3F8"/>
    <dbReference type="GeneID" id="108258080"/>
    <dbReference type="KEGG" id="ipu:108258080"/>
    <dbReference type="CTD" id="124512"/>
    <dbReference type="OrthoDB" id="407325at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF164">
        <property type="entry name" value="HISTONE-ARGININE METHYLTRANSFERASE METTL23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00043988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017311900.1"/>
        </source>
    </evidence>
    <sequence length="241" mass="26945" checksum="DAABFFD57673609C" modified="2017-12-20" version="1">MSLPIKIRICNVDESANPRFIQKTFTFENHDGTHVLMVSVPEVVKILGLQYGMYVWPCAVVLAQYVWTTREKIQRKTVLELGAGVSLPGIVAAKCGAHVILSDSAEIPLCLENSRRCCELNDLPGVPVVGLTWGEISPELRSLPPVDIILGSDVFYEPEDFEDVLVTLSFLLKRNPSGQFWTTFQERSSDWTIETLLNKWNLQCTDVPLDIFHANKKHLAGSTLPGNCSIQMMIVTARTKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q512</accession>
    <name>A0A2D0Q512_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein wu:fa56d06</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">wu:fa56d06</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017313389.1">
        <property type="nucleotide sequence ID" value="XM_017457900.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q512"/>
    <dbReference type="GeneID" id="108258903"/>
    <dbReference type="KEGG" id="ipu:108258903"/>
    <dbReference type="OrthoDB" id="8960263at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013016943" evidence="1">
        <location>
            <begin position="17"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017313389.1"/>
        </source>
    </evidence>
    <sequence length="535" mass="61496" checksum="E5E147FD8BD1C8D3" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q5Z6</accession>
    <name>A0A2D0Q5Z6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transportin-2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC108258583</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017312780.1">
        <property type="nucleotide sequence ID" value="XM_017457291.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q5Z6"/>
    <dbReference type="GeneID" id="108258583"/>
    <dbReference type="KEGG" id="ipu:108258583"/>
    <dbReference type="CTD" id="100005536"/>
    <dbReference type="OrthoDB" id="951172at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="325"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="351"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017312780.1"/>
        </source>
    </evidence>
    <sequence length="891" mass="100577" checksum="382A5F5D3744E5E3" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q8W2</accession>
    <name>A0A2D0Q8W2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Borealin-2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">cdca9</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the borealin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017314727.1">
        <property type="nucleotide sequence ID" value="XM_017459238.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q8W2"/>
    <dbReference type="GeneID" id="108259614"/>
    <dbReference type="KEGG" id="ipu:108259614"/>
    <dbReference type="CTD" id="100151622"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032133">
        <property type="term" value="C:chromosome passenger complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051233">
        <property type="term" value="C:spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018851">
        <property type="entry name" value="Borealin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018867">
        <property type="entry name" value="Cell_div_borealin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16040">
        <property type="entry name" value="AUSTRALIN, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16040:SF10">
        <property type="entry name" value="BOREALIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10444">
        <property type="entry name" value="Nbl1_Borealin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Borealin N-terminal" evidence="12">
        <location>
            <begin position="29"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="102"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="33"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="119"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF10444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017314727.1"/>
        </source>
    </evidence>
    <sequence length="253" mass="28209" checksum="CC06B9DACC804A85" modified="2017-12-20" version="1">MAARRTRKGSQSSEGVQRDQAADKLRKRKELFIQQFEKEAQERINEMEAKLDKLLATLDRAVKIEMMKLPQSLHTTLMKDILSAQETSVGEVTMVIGSVSPEISKPLSRKPSKKGKASTPMTAAQRSTGQVKTDDDNKRRPRKKMQPSNSTGNLRCVSTVGAKRTQGRVLKLSDQALRLGGNMKLLSRSLSDDLSGLATATITTSLGETLFLSEENKDEVKLELLDEMALCQMQKIKELMEYLCNKVKLSNTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QDH7</accession>
    <name>A0A2D0QDH7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PrdX deacylase domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC108260508</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PRORSD1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017316354.1">
        <property type="nucleotide sequence ID" value="XM_017460865.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QDH7"/>
    <dbReference type="GeneID" id="108260508"/>
    <dbReference type="KEGG" id="ipu:108260508"/>
    <dbReference type="OMA" id="AGAHTKN"/>
    <dbReference type="OrthoDB" id="424586at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04335">
        <property type="entry name" value="PrdX_deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.960.10:FF:000005">
        <property type="entry name" value="Putative prolyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.960.10">
        <property type="entry name" value="YbaK/aminoacyl-tRNA synthetase-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040285">
        <property type="entry name" value="ProX/PRXD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036754">
        <property type="entry name" value="YbaK/aa-tRNA-synt-asso_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007214">
        <property type="entry name" value="YbaK/aa-tRNA-synth-assoc-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31423:SF3">
        <property type="entry name" value="PROLYL-TRNA SYNTHETASE ASSOCIATED DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31423">
        <property type="entry name" value="YBAK DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04073">
        <property type="entry name" value="tRNA_edit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55826">
        <property type="entry name" value="YbaK/ProRS associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YbaK/aminoacyl-tRNA synthetase-associated" evidence="3">
        <location>
            <begin position="26"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00031612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017316354.1"/>
        </source>
    </evidence>
    <sequence length="170" mass="19126" checksum="9B434738CD6B268E" modified="2017-12-20" version="1">MGSAELRMELENLLKSLNIDFVSVEHPEVFTVEEMMPHVQHLNGAVTKNLFLKDKKKKSLWLVSVRHDRQVNLNDLAKKLGVGSGNLRFADEAAMLEKLRVGQGCATALALFCDKDRSVRFILDSDLADGGHERVYFHPMTNAATLGLKPDDLMRFLKETGHEPILHSFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QE08</accession>
    <name>A0A2D0QE08_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pleckstrin homology-like domain family B member 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Protein LL5-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">phldb1a</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
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            <taxon>Vertebrata</taxon>
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            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="17">SDA103</strain>
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="DELETED IN MALIGNANT BRAIN TUMORS 1 PROTEIN"/>
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    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
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            <begin position="1"/>
            <end position="28"/>
        </location>
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            <end position="822"/>
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    <feature type="domain" description="Kringle" evidence="14">
        <location>
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        </location>
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    <feature type="domain" description="SRCR" evidence="16">
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        </location>
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            <end position="204"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="153"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="184"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="250"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="263"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="294"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="400"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="470"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="483"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="514"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017317167.1"/>
        </source>
    </evidence>
    <sequence length="822" mass="89513" checksum="C16D0A25DB844E93" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QG51</accession>
    <name>A0A2D0QG51_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Lysophospholipid acyltransferase LPCAT4 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">lpcat4</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13">Phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017317294.1">
        <property type="nucleotide sequence ID" value="XM_017461805.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QG51"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000014507"/>
    <dbReference type="GeneID" id="108261003"/>
    <dbReference type="KEGG" id="ipu:108261003"/>
    <dbReference type="CTD" id="254531"/>
    <dbReference type="OMA" id="HEYPHPF"/>
    <dbReference type="OrthoDB" id="272512at2759"/>
    <dbReference type="UniPathway" id="UPA00085"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047184">
        <property type="term" value="F:1-acylglycerophosphocholine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042171">
        <property type="term" value="F:lysophosphatidic acid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036151">
        <property type="term" value="P:phosphatidylcholine acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07991">
        <property type="entry name" value="LPLAT_LPCAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045252">
        <property type="entry name" value="LPCAT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063:SF7">
        <property type="entry name" value="LYSOPHOSPHOLIPID ACYLTRANSFERASE LPCAT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063">
        <property type="entry name" value="PHOSPHOLIPID ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 18" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="32"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="400"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="487"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="497"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017317294.1"/>
        </source>
    </evidence>
    <sequence length="514" mass="57525" checksum="97F6D3D805C57985" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QHA3</accession>
    <name>A0A2D0QHA3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="7">PPIase</shortName>
            <ecNumber evidence="7">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC108261251</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="7"/>
    <dbReference type="RefSeq" id="XP_017317639.1">
        <property type="nucleotide sequence ID" value="XM_017462150.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QHA3"/>
    <dbReference type="GeneID" id="108261251"/>
    <dbReference type="KEGG" id="ipu:108261251"/>
    <dbReference type="OrthoDB" id="2083at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035542">
        <property type="entry name" value="CRIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45843">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45843:SF1">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 7" id="KW-0697">Rotamase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="9">
        <location>
            <begin position="6"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017317639.1"/>
        </source>
    </evidence>
    <sequence length="123" mass="14148" checksum="03B411EB356BC6D1" modified="2017-12-20" version="1">MAVLLETTLGDIVVDLYTEERPKACLNFLKLCKIKYYNYCLIHNVQRDFIVQTGDPTGSGRGGESVFCKLYGDQARFFDAEKVPRIKHKKKGTVSMVNNGNEQHGSQVLILYIYIIFIYLYTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QHD8</accession>
    <name>A0A2D0QHD8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Methylsterol monooxygenase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">msmo1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017316879.1">
        <property type="nucleotide sequence ID" value="XM_017461390.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QHD8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003865"/>
    <dbReference type="GeneID" id="100304913"/>
    <dbReference type="KEGG" id="ipu:100304913"/>
    <dbReference type="CTD" id="6307"/>
    <dbReference type="OMA" id="IVHEFIY"/>
    <dbReference type="OrthoDB" id="1658724at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="5">
        <location>
            <begin position="144"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017316879.1"/>
        </source>
    </evidence>
    <sequence length="291" mass="34729" checksum="026DAB467B5EFAAB" modified="2017-12-20" version="1">METNGTADILSSAYLAVELVDSLLPQNPLQEPVKQAWNHMLQNYSKFQIATWGSLIVHELVYFLFCLPGFVFQFIPFMQRYKIQKDKPETWEKQWRCVKMLLFNHFCIQLPLICGTYYFTEYFNIPYDWESMPRWPYLLAQCFGCAVIEDTWHYFLHRALHHRRIYKYIHKVHHDFTAPFGMQAEYAHPLETLILGAGFFIGIMVFCNHVALLWAWVTIRLLETIDVHSGYDIPLNPLHLIPFYAGARFHDFHHMNFVGNYGSTFTWWDRLFNTDDQYNSYTQKQNLKKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QNT2</accession>
    <name>A0A2D0QNT2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Calphotin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC108263264</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017319391.2">
        <property type="nucleotide sequence ID" value="XM_017463902.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QNT2"/>
    <dbReference type="GeneID" id="108263264"/>
    <dbReference type="KEGG" id="ipu:108263264"/>
    <dbReference type="OrthoDB" id="19742at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12379">
        <property type="entry name" value="RRM2_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045305">
        <property type="entry name" value="RRM2_I_PABPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="7"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="95"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017319391.2"/>
        </source>
    </evidence>
    <sequence length="702" mass="74393" checksum="A3776E5E43E04533" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QSF8</accession>
    <name>A0A2D0QSF8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6 7">Ankyrin repeat domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6 7" type="primary">ankrd67</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5 6 7" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5 6 7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017321228.1">
        <property type="nucleotide sequence ID" value="XM_017465739.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017321229.1">
        <property type="nucleotide sequence ID" value="XM_017465740.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534164.1">
        <property type="nucleotide sequence ID" value="XM_053678189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QSF8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022835"/>
    <dbReference type="GeneID" id="108264315"/>
    <dbReference type="KEGG" id="ipu:108264315"/>
    <dbReference type="CTD" id="566073"/>
    <dbReference type="GeneTree" id="ENSGT00940000162060"/>
    <dbReference type="OMA" id="WAVFGGW"/>
    <dbReference type="OrthoDB" id="20872at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126:SF14">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126">
        <property type="entry name" value="ANKYRIN REPEAT, PH AND SEC7 DOMAIN CONTAINING PROTEIN SECG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="121"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="154"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="187"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="220"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="253"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="286"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="319"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="352"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017321228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017321229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053534164.1"/>
        </source>
    </evidence>
    <sequence length="427" mass="46697" checksum="268FA6D35D536203" modified="2017-12-20" version="1">MAFSGPELQWKWLELHDLVSLRAVERKIHTAAGAAVRPRQWRLHSRPRAQHSRLDEHKLHYAAWDGRVEHVKALLDCGVPANCPDPRGWTAVHHAAFRGHLALLRFLLHASKMDVNCPDLFGCTPLHRSCMGGNKDATEYLLQKGASHEARSCCGQTPLHLAAAGGHLGTARMLLQHLASPDVLDSRRWTPLHWAVFGGWGDVAELLLDNGAELEGPSGVGTSPLQLAVMVGNEAAVRLLLHRGADANLRGSTGRTALHLCACSAEKKILQHLLAAGAKVCVRDRDQAAPLHLAALSGSSAVLHLLILNGAGLEDRDSLHMTPLHYSTLKDNAEAAKLLLHYGAHVDATERLGRTPLHLATERGHCKVLSVLLERGADPSIKTKWRETAEDVARTHKQYGSLKVLQEHRKPPVRSQTLGHTVTESVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QV66</accession>
    <name>A0A2D0QV66_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sulfotransferase</fullName>
            <ecNumber evidence="6">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">st1s3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="6"/>
    <dbReference type="RefSeq" id="XP_017322312.1">
        <property type="nucleotide sequence ID" value="XM_017466823.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QV66"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033331"/>
    <dbReference type="GeneID" id="100528237"/>
    <dbReference type="CTD" id="100528237"/>
    <dbReference type="OMA" id="IRERNIF"/>
    <dbReference type="OrthoDB" id="205623at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006584">
        <property type="term" value="P:catecholamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000433">
        <property type="entry name" value="Estrogen sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0128">Catecholamine metabolism</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="7">
        <location>
            <begin position="43"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017322312.1"/>
        </source>
    </evidence>
    <sequence length="301" mass="35375" checksum="BDC50E219A5E100F" modified="2017-12-20" version="1">MEGVNTSSMKPTCRPELFDFEGISMVHYFTDNWEKVQKFQARPNDILIATYPKAGTTWVSYILDLLYFHNSAPERQTSLPIFVRVPFLEAVFPEMPTGVDLADKLPNTPRLIKTHLPVQLVPKSFWEQNCKVVYVARNAKDNAVSYFHFARMINLLPEPGNWNTFLQSFMDGKLVSGPWYDHVTGYWEKKQTYSNLHYMFFEDMVANTEHELEQLCSFLGLSTPAEERERITKCVHFDVMKQNNMTNHSSFSHMDFKISPFMRKGKVGDWKNHFTVAQNERFDEHYWQKMKNTTLQFHTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QYI1</accession>
    <name>A0A2D0QYI1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Activity-dependent neuroprotective protein 2a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">adnp2a</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017322811.2">
        <property type="nucleotide sequence ID" value="XM_017467322.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QYI1"/>
    <dbReference type="GeneID" id="108265191"/>
    <dbReference type="KEGG" id="ipu:108265191"/>
    <dbReference type="CTD" id="100007394"/>
    <dbReference type="OrthoDB" id="10053955at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038861">
        <property type="entry name" value="ADNP/ADNP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045762">
        <property type="entry name" value="ADNP_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15740:SF2">
        <property type="entry name" value="ACTIVITY-DEPENDENT NEUROPROTECTOR HOMEOBOX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15740">
        <property type="entry name" value="NEUROPROTECTIVE PEPTIDE-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19627">
        <property type="entry name" value="ADNP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="238"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="577"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="680"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="830"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="503"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="503"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017322811.2"/>
        </source>
    </evidence>
    <sequence length="914" mass="103155" checksum="49DB20066D927C31" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R0M2</accession>
    <name>A0A2D0R0M2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Dual specificity protein phosphatase CDC14B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">cdc14b</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 22">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 22">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cell projection</location>
            <location evidence="14">Kinocilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle pole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017323325.2">
        <property type="nucleotide sequence ID" value="XM_017467836.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R0M2"/>
    <dbReference type="GeneID" id="108265480"/>
    <dbReference type="KEGG" id="ipu:108265480"/>
    <dbReference type="CTD" id="8555"/>
    <dbReference type="OrthoDB" id="266663at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060091">
        <property type="term" value="C:kinocilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14499">
        <property type="entry name" value="CDC14_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17657">
        <property type="entry name" value="CDC14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000032">
        <property type="entry name" value="dual specificity protein phosphatase CDC14A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000006">
        <property type="entry name" value="Dual specificity protein phosphatase CDC14B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044506">
        <property type="entry name" value="CDC14_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029260">
        <property type="entry name" value="DSPn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050561">
        <property type="entry name" value="PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23339">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASE AND DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14671">
        <property type="entry name" value="DSPn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="18">
        <location>
            <begin position="196"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="19">
        <location>
            <begin position="278"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="443"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017323325.2"/>
        </source>
    </evidence>
    <sequence length="573" mass="65108" checksum="14FB0EB901F5AADE" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R1I8</accession>
    <name>A0A2D0R1I8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Homeobox protein Nkx-3.1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">nkx3-1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017324419.1">
        <property type="nucleotide sequence ID" value="XM_017468930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R1I8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000011476"/>
    <dbReference type="GeneID" id="108266005"/>
    <dbReference type="KEGG" id="ipu:108266005"/>
    <dbReference type="CTD" id="4824"/>
    <dbReference type="OMA" id="FCKAYQY"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050394">
        <property type="entry name" value="Homeobox_NK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340">
        <property type="entry name" value="HOMEOBOX PROTEIN NKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24340:SF38">
        <property type="entry name" value="HOMEOBOX PROTEIN NKX-3.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="97"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="99"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="66"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="69"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017324419.1"/>
        </source>
    </evidence>
    <sequence length="215" mass="24434" checksum="EE67559D2BCD9C4D" modified="2017-12-20" version="1">MASSNRQLTSFFIEDILSMREEKGEERGEETRGSSAESQIDSRCCKDGGENQDALLIAALATRPASPVDSCTSERTNSPTEAATHADEPNHTHTLAQKQKRSRAAFTHLQVLELEKKFSRQRYLSAPERAHLASALRLTETQVKIWFQNRRYKTKRRQQLDCYRKSEGNAAVAAAATVDEDLLRASLLATMYTCYPYRLCVYDPHRHGAWRPTVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R531</accession>
    <name>A0A2D0R531_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="21 22 23">Bromodomain adjacent to zinc finger domain protein 2B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21 22 23 24" type="primary">LOC108266638</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21 22 23" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21 22 23">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WAL family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017325708.1">
        <property type="nucleotide sequence ID" value="XM_017470219.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017325710.1">
        <property type="nucleotide sequence ID" value="XM_017470221.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017325711.1">
        <property type="nucleotide sequence ID" value="XM_017470222.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053537032.1">
        <property type="nucleotide sequence ID" value="XM_053681057.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108266638"/>
    <dbReference type="KEGG" id="ipu:108266638"/>
    <dbReference type="OrthoDB" id="21449at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05503">
        <property type="entry name" value="Bromo_BAZ2A_B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01397">
        <property type="entry name" value="HAT_MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15630">
        <property type="entry name" value="PHD_BAZ2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000199">
        <property type="entry name" value="Bromodomain adjacent to zinc finger domain 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.890.10:FF:000002">
        <property type="entry name" value="Bromodomain adjacent to zinc finger domain protein 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.890.10">
        <property type="entry name" value="Methyl-cpg-binding Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037374">
        <property type="entry name" value="BAZ2A/B_Bromo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018501">
        <property type="entry name" value="DDT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001739">
        <property type="entry name" value="Methyl_CpG_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028942">
        <property type="entry name" value="WHIM1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028941">
        <property type="entry name" value="WHIM2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45915:SF1">
        <property type="entry name" value="BROMODOMAIN ADJACENT TO ZINC FINGER DOMAIN PROTEIN 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45915">
        <property type="entry name" value="TRANSCRIPTION INTERMEDIARY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02791">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01429">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15612">
        <property type="entry name" value="WHIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15613">
        <property type="entry name" value="WSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00571">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00391">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="10">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the lipoxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017327615.1">
        <property type="nucleotide sequence ID" value="XM_017472126.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RCK5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006759"/>
    <dbReference type="GeneID" id="108267745"/>
    <dbReference type="KEGG" id="ipu:108267745"/>
    <dbReference type="CTD" id="239"/>
    <dbReference type="OMA" id="MEMIMAS"/>
    <dbReference type="OrthoDB" id="407298at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034440">
        <property type="term" value="P:lipid oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01753">
        <property type="entry name" value="PLAT_LOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.245.10:FF:000001">
        <property type="entry name" value="Arachidonate 5-lipoxygenase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.60.20:FF:000002">
        <property type="entry name" value="Arachidonate 5-lipoxygenase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.245.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000907">
        <property type="entry name" value="LipOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013819">
        <property type="entry name" value="LipOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036226">
        <property type="entry name" value="LipOase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020834">
        <property type="entry name" value="LipOase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001885">
        <property type="entry name" value="LipOase_mml"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042062">
        <property type="entry name" value="PLAT_LOX_verte"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11771">
        <property type="entry name" value="LIPOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00305">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00087">
        <property type="entry name" value="LIPOXYGENASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00467">
        <property type="entry name" value="MAMLPOXGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48484">
        <property type="entry name" value="Lipoxigenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00081">
        <property type="entry name" value="LIPOXYGENASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51393">
        <property type="entry name" value="LIPOXYGENASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8 10" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLAT" evidence="14">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoxygenase" evidence="15">
        <location>
            <begin position="117"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="366"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="546"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="669"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Essential for stabilizing binding to COTL1" evidence="12">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601885-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601885-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601885-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017327615.1"/>
        </source>
    </evidence>
    <sequence length="669" mass="76964" checksum="9AB80F4A240F6EFF" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RFQ5</accession>
    <name>A0A2D0RFQ5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein LOC108268740</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC108268740</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017329369.2">
        <property type="nucleotide sequence ID" value="XM_017473880.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RFQ5"/>
    <dbReference type="GeneID" id="108268740"/>
    <dbReference type="KEGG" id="ipu:108268740"/>
    <dbReference type="OrthoDB" id="8947657at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF133">
        <property type="entry name" value="IMMUNE-TYPE RECEPTOR 5 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="118"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017329369.2"/>
        </source>
    </evidence>
    <sequence length="286" mass="31312" checksum="E1FFA75BE725C710" modified="2023-06-28" version="2">MNIVNQPNPVITAASGDNVTLHCLRVEEGDTDPIIWYKQALGHEPRAMVTVQKVVKNPIFEDQFNSPRFTVENLIGSCNLKITNVETSDEAVYYCGLKKFAIAFGKGTFLSVKGDGDVKVSVFQSGVSDSVPAGASVTLQCSVLSESRSADLQVLWFRAAPPQSHPQIIYTHHNSSHQCESGSPTHTCVYNFSKNILSLSDTGTYYCAVAVCGKIIFGNGTRVQLERSVDPVVIYLAVALGVCVVVIFALIFVLTCERKNFDQRTVRLKQGSLTDKTLDQVNIQDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RG82</accession>
    <name>A0A2D0RG82_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">KxDL motif-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 7 8 9 10 11" type="primary">kxd1</name>
    </gene>
    <organism evidence="5 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6 7 8" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6 7 8">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the KXD1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017329543.2">
        <property type="nucleotide sequence ID" value="XM_017474054.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017329545.2">
        <property type="nucleotide sequence ID" value="XM_017474056.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017329546.2">
        <property type="nucleotide sequence ID" value="XM_017474057.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047013192.2">
        <property type="nucleotide sequence ID" value="XM_047157236.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047013193.2">
        <property type="nucleotide sequence ID" value="XM_047157237.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053538355.1">
        <property type="nucleotide sequence ID" value="XM_053682380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RG82"/>
    <dbReference type="GeneID" id="108268823"/>
    <dbReference type="KEGG" id="ipu:108268823"/>
    <dbReference type="CTD" id="79036"/>
    <dbReference type="GeneTree" id="ENSGT00530000064192"/>
    <dbReference type="OrthoDB" id="10258877at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099078">
        <property type="term" value="C:BORC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032418">
        <property type="term" value="P:lysosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039843">
        <property type="entry name" value="KXD1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019371">
        <property type="entry name" value="KxDL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13511">
        <property type="entry name" value="KXDL MOTIF-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13511:SF0">
        <property type="entry name" value="KXDL MOTIF-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10241">
        <property type="entry name" value="KxDL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KxDL" evidence="4">
        <location>
            <begin position="12"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="116"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="131"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="166"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017329543.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017329545.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017329546.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_047013192.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_047013193.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053538355.1"/>
        </source>
    </evidence>
    <sequence length="192" mass="21492" checksum="6E8FF4EA59584477" modified="2023-06-28" version="2">MDPTASGIFCSRMLSMVNSEDVNAIIQAQRHMLDRFEKTNEMLINFNGLSNVRLQQMNDRFLLHTRTLIEMKKDLDSVFRRIRTLKGKIAKQYPNAFSNIAQHRSLYSSTDIREASSLEDDDDDFDPVGPSVATTTATSEQSTESCDTSPDIISPTISRCSEELSQENPDTPTSNSPERAVLLDEGPDSVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RGA0</accession>
    <name>A0A2D0RGA0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Fermitin family homolog 3b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">fermt3b</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5 6">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the kindlin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017329570.1">
        <property type="nucleotide sequence ID" value="XM_017474081.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017329572.1">
        <property type="nucleotide sequence ID" value="XM_017474083.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RGA0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003603"/>
    <dbReference type="GeneID" id="108268835"/>
    <dbReference type="KEGG" id="ipu:108268835"/>
    <dbReference type="CTD" id="393878"/>
    <dbReference type="OMA" id="WELRAFV"/>
    <dbReference type="OrthoDB" id="10057618at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030055">
        <property type="term" value="C:cell-substrate junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033622">
        <property type="term" value="P:integrin activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007159">
        <property type="term" value="P:leukocyte cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070527">
        <property type="term" value="P:platelet aggregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033632">
        <property type="term" value="P:regulation of cell-cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13205">
        <property type="entry name" value="FERM_C_fermitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17185">
        <property type="entry name" value="FERM_F1_KIND3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01237">
        <property type="entry name" value="PH_fermitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000037">
        <property type="entry name" value="Fermitin family homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037843">
        <property type="entry name" value="Kindlin/fermitin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040790">
        <property type="entry name" value="Kindlin_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037837">
        <property type="entry name" value="PH_Kindlin/fermitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16160">
        <property type="entry name" value="FERMITIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16160:SF1">
        <property type="entry name" value="FERMITIN FAMILY HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18124">
        <property type="entry name" value="Kindlin_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="340"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017329570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017329572.1"/>
        </source>
    </evidence>
    <sequence length="640" mass="73622" checksum="2D4A4910FBDB7EB7" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RGA5</accession>
    <name>A0A2D0RGA5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Trafficking kinesin-binding protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">trak1a</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9">SDA103</strain>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <property type="project" value="GOC"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="TRAK1/2_C"/>
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        <property type="entry name" value="TRAFFICKING KINESIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="RefSeq" id="XP_017329577.1"/>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
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                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC108269105</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the POU transcription factor family. Class-3 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017330163.1">
        <property type="nucleotide sequence ID" value="XM_017474674.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RI05"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021341"/>
    <dbReference type="GeneID" id="108269105"/>
    <dbReference type="KEGG" id="ipu:108269105"/>
    <dbReference type="OrthoDB" id="6358449at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000005">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000001">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016362">
        <property type="entry name" value="TF_POU_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF83">
        <property type="entry name" value="POU DOMAIN, CLASS 3, TRANSCRIPTION FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00157">
        <property type="entry name" value="Pou"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002629">
        <property type="entry name" value="Transcription_factor_POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00028">
        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00352">
        <property type="entry name" value="POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00035">
        <property type="entry name" value="POU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00465">
        <property type="entry name" value="POU_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="POU-specific" evidence="14">
        <location>
            <begin position="177"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="267"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="269"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="134"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="324"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="341"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017330163.1"/>
        </source>
    </evidence>
    <sequence length="353" mass="38732" checksum="3FFB04BF0B755D35" modified="2017-12-20" version="1">MATAASNPYSILTSTSMVHPDASVMQQGGSPFRNHQKLLQSDYLQGVQSNGHPIGHQWVSNLAEGSPWSASLEQQDVKPGREDLQLGAIIHHRSTAHVAHHSPHTNHHAGAWGTPASHNSSSINIYSQTGFSVNGMHGGLTPPPPSSAPTQSLHPGLRETPEPGDLGAHHCHDHSDEETPTSDELEQFAKQFKQRRIKLGFTQADVGLALGTLYGNVFSQTTICRFEALQLSFKNMCKLKPLLNKWLEEADSSTGSPSSIDKIAAQGRKRKKRTSIEVSVKGVLETHFLKCPKPAAQEISSLADSLQLEKEVVRVWFCNRRQKEKRMTPPGEQQGHEVYSHTVNTDSSSCRDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RLH4</accession>
    <name>A0A2D0RLH4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">G2/M phase-specific E3 ubiquitin-protein ligase isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">g2e3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017330800.1">
        <property type="nucleotide sequence ID" value="XM_017475311.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RLH4"/>
    <dbReference type="GeneID" id="108269435"/>
    <dbReference type="KEGG" id="ipu:108269435"/>
    <dbReference type="CTD" id="55632"/>
    <dbReference type="OrthoDB" id="512616at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15496">
        <property type="entry name" value="PHD_PHF7_G2E3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051188">
        <property type="entry name" value="PHD-type_Zinc_Finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059102">
        <property type="entry name" value="PHD_PHF7/G2E3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12420:SF42">
        <property type="entry name" value="G2_M PHASE-SPECIFIC E3 UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12420">
        <property type="entry name" value="PHD FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26054">
        <property type="entry name" value="PHD_G2E3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13771">
        <property type="entry name" value="zf-HC5HC2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="8">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="7">
        <location>
            <begin position="581"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017330800.1"/>
        </source>
    </evidence>
    <sequence length="652" mass="73709" checksum="A7ACD8E8CB8B21B5" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RMP2</accession>
    <name>A0A2D0RMP2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">DnaJ (Hsp40) homolog, subfamily C, member 5 gamma b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">dnajc5gb</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8 9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017331781.1">
        <property type="nucleotide sequence ID" value="XM_017476292.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017331782.1">
        <property type="nucleotide sequence ID" value="XM_017476293.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RMP2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005112"/>
    <dbReference type="GeneID" id="108270045"/>
    <dbReference type="KEGG" id="ipu:108270045"/>
    <dbReference type="CTD" id="393371"/>
    <dbReference type="GeneTree" id="ENSGT00940000164252"/>
    <dbReference type="OMA" id="FIMNSCW"/>
    <dbReference type="OrthoDB" id="445556at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000017">
        <property type="entry name" value="dnaJ homolog subfamily C member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051434">
        <property type="entry name" value="DnaJ_C_subfamily_member5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44027">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44027:SF2">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 5G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0564">Palmitate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="6">
        <location>
            <begin position="17"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017331781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017331782.1"/>
        </source>
    </evidence>
    <sequence length="199" mass="22394" checksum="4B58FD5A34A9FC8E" modified="2017-12-20" version="1">MTDPNKPQRKLSRSGDSLYKTLGLQKGASSEDIKKAYRKLALRYHPDKNPENPEAAEKFKEINSANSILNDENKRQIYDEYGSMGLYVADQFGEDSVKYYFLMSKCWFKTLFFCAIIFTGCCCCCCCCFCCGKCGSKEEDEHYFYMDPEDLEAQVFEEQNKGNQTVIIGQPVPSAASEVSPQGPVTVGMPISSPDTMNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RNC6</accession>
    <name>A0A2D0RNC6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">non-specific protein-tyrosine kinase</fullName>
            <ecNumber evidence="5">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24 25" type="primary">ptk2ba</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24 25" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24 25">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017332008.1">
        <property type="nucleotide sequence ID" value="XM_017476519.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017332009.1">
        <property type="nucleotide sequence ID" value="XM_017476520.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RNC6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005670"/>
    <dbReference type="GeneID" id="108270138"/>
    <dbReference type="KEGG" id="ipu:108270138"/>
    <dbReference type="CTD" id="557258"/>
    <dbReference type="GeneTree" id="ENSGT00940000157269"/>
    <dbReference type="OrthoDB" id="9976756at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007172">
        <property type="term" value="P:signal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13190">
        <property type="entry name" value="FERM_C_FAK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000058">
        <property type="entry name" value="focal adhesion kinase 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000004">
        <property type="entry name" value="Protein-tyrosine kinase 2-beta isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.330:FF:000007">
        <property type="entry name" value="protein-tyrosine kinase 2-beta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000080">
        <property type="entry name" value="protein-tyrosine kinase 2-beta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000194">
        <property type="entry name" value="protein-tyrosine kinase 2-beta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000027">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041390">
        <property type="entry name" value="FADK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049385">
        <property type="entry name" value="FAK1-like_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041784">
        <property type="entry name" value="FAK1/PYK2_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036137">
        <property type="entry name" value="Focal_adhe_kin_target_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005189">
        <property type="entry name" value="Focal_adhesion_kin_target_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46221">
        <property type="entry name" value="FERM AND PDZ DOMAIN-CONTAINING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46221:SF11">
        <property type="entry name" value="NON-SPECIFIC PROTEIN-TYROSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21477">
        <property type="entry name" value="FERM_C_FAK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18038">
        <property type="entry name" value="FERM_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03623">
        <property type="entry name" value="Focal_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68993">
        <property type="entry name" value="FAT domain of focal adhesion kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 24 25" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="15 24 25" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="FERM" evidence="22">
        <location>
            <begin position="42"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="424"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="700"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="821"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="851"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="456"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00061333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_017332008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_017332009.1"/>
        </source>
    </evidence>
    <sequence length="998" mass="113881" checksum="E0A118DABD0862AA" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RNG4</accession>
    <name>A0A2D0RNG4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">[histone H3]-lysine(4) N-trimethyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.354</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">smyd2a</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically trimethylates histone H3 'Lys-4' (H3K4me3) in vivo. The activity requires interaction with HSP90alpha. Shows even higher methyltransferase activity on p53/TP53. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at 'Lys-860'.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60260"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.354"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-lysyl-[protein] + S-adenosyl-L-methionine = N(6)-methyl-L-lysyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51736"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13053"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.354" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017331500.1">
        <property type="nucleotide sequence ID" value="XM_017476011.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RNG4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022187"/>
    <dbReference type="GeneID" id="108269891"/>
    <dbReference type="KEGG" id="ipu:108269891"/>
    <dbReference type="CTD" id="541423"/>
    <dbReference type="OMA" id="HRIMDYL"/>
    <dbReference type="OrthoDB" id="5945798at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140999">
        <property type="term" value="F:histone H3K4 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060047">
        <property type="term" value="P:heart contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000013">
        <property type="entry name" value="Histone-lysine N-methyltransferase SMYD1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.970:FF:000002">
        <property type="entry name" value="N-lysine methyltransferase SMYD2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.140.2220:FF:000013">
        <property type="entry name" value="N-lysine methyltransferase SMYD2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050869">
        <property type="entry name" value="H3K4_H4K5_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197:SF193">
        <property type="entry name" value="N-LYSINE METHYLTRANSFERASE SMYD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 22" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="19">
        <location>
            <begin position="7"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="MYND-type" evidence="20">
        <location>
            <begin position="52"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017331500.1"/>
        </source>
    </evidence>
    <sequence length="435" mass="49903" checksum="05BD940EBD23E8BB" modified="2017-12-20" version="1">MKSEGIEGCERFLSPGKGRGVRATKHFQVGDLVFACPAYSYVLTVNERGSHCEFCFTRKEGLSKCGKCKQAYYCNVECQKGDWSMHKLECSAMCSYGENWCPSETVRLVARIIMKQKFQTELTQSERLLSLKEFEAHLDKIDNEKNEMNQTDIAALHHFYSKHLDIPSNAALAELFAQVNCNGFTIEDEELSHLGSAVFPDVALMNHSCNPNVIVTYKGTVAEVRAVQKISPGEEIFNSYIDLLYSTEDRNERLKDSYFFTCDCTECTTKSKDKAKIKIRQKLSTTPEPEEIQEMVRYAKNIIEEFRRAKHYKTPSELLEICELSLEKMGSIFEDTNVYMLHMMYQAMGVCLYMQDWDGAMKYGEKIVHPYSVHYPQYSLNVASMYLKLGRLYLGLEKKPQGVKALKKAIAIMNVAFGKDHHYIAEVKKEMEEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RSV8</accession>
    <name>A0A2D0RSV8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Centriolar and ciliogenesis-associated protein HYSL1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">hyls1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HYLS1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017333250.1">
        <property type="nucleotide sequence ID" value="XM_017477761.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RSV8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000017201"/>
    <dbReference type="GeneID" id="108270815"/>
    <dbReference type="KEGG" id="ipu:108270815"/>
    <dbReference type="CTD" id="219844"/>
    <dbReference type="OMA" id="YDARQEY"/>
    <dbReference type="OrthoDB" id="6343432at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097730">
        <property type="term" value="C:non-motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052319">
        <property type="entry name" value="Centriolar_ciliogenesis_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026227">
        <property type="entry name" value="HYLS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027918">
        <property type="entry name" value="HYLS1_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34174:SF1">
        <property type="entry name" value="CENTRIOLAR AND CILIOGENESIS-ASSOCIATED PROTEIN HYLS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34174">
        <property type="entry name" value="HYDROLETHALUS SYNDROME PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15311">
        <property type="entry name" value="HYLS1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02098">
        <property type="entry name" value="HYLETHALUSS1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Centriolar and ciliogenesis-associated protein HYLS1 C-terminal" evidence="9">
        <location>
            <begin position="261"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="43"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="170"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="183"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF15311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017333250.1"/>
        </source>
    </evidence>
    <sequence length="360" mass="41578" checksum="35A77FE4B2E88849" modified="2017-12-20" version="1">MENLDFSEEEIQEQLAVLGYTNITKERLREFKQDLDLLIRHEKSRSSTSAERSSPESHRSSSKSPPAFTKEKVQVPGAGSKYSYIYSSNRAADQHRQVLTSIFDDENRDSYIKHSVAPRCMRPSTAPNRLEVEDASSDVYPSVLGAGEPVRQQRDLHTNAKPALKRKVLRKHRGHSQVCDESTYSEDSEPVSEMEERLDHLRVSAPHARHDSYSESEETGSYSDRRSSATVELPSAFQFYIKGMTRSRSENDIRPRPKSFIRPVMDHPHTRNLKKSDPVAKYFQYKHEWETFKAAGEIRRKELHWAIRDQLAYQPPPPKPQRTYIPNTYVVPTEKKRSSLRWEIRHDLANGIIPAKATYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RVI3</accession>
    <name>A0A2D0RVI3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">RNA-binding protein MEX3B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">mex3b</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017334504.1">
        <property type="nucleotide sequence ID" value="XM_017479015.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RVI3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034766"/>
    <dbReference type="GeneID" id="108271418"/>
    <dbReference type="KEGG" id="ipu:108271418"/>
    <dbReference type="CTD" id="84206"/>
    <dbReference type="OMA" id="QFERGPA"/>
    <dbReference type="OrthoDB" id="427410at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22423">
        <property type="entry name" value="KH-I_MEX3_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22424">
        <property type="entry name" value="KH-I_MEX3_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000013">
        <property type="entry name" value="Mex-3 RNA-binding family member B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000090">
        <property type="entry name" value="Mex-3 RNA-binding family member C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000012">
        <property type="entry name" value="Mex-3 RNA-binding family member D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047228">
        <property type="entry name" value="KH-I_MEX3_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047226">
        <property type="entry name" value="KH-I_MEX3_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047227">
        <property type="entry name" value="MEX3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23285">
        <property type="entry name" value="RING FINGER AND KH DOMAIN CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23285:SF5">
        <property type="entry name" value="RNA-BINDING PROTEIN MEX3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="483"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="270"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="402"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="454"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017334504.1"/>
        </source>
    </evidence>
    <sequence length="534" mass="56848" checksum="4F3FA1C870882971" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RWH9</accession>
    <name>A0A2D0RWH9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">CCAAT/enhancer binding protein (C/EBP) 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">cebp1</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6 7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bZIP family. C/EBP subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017334532.1">
        <property type="nucleotide sequence ID" value="XM_017479043.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017334541.1">
        <property type="nucleotide sequence ID" value="XM_017479052.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RWH9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000036991"/>
    <dbReference type="GeneID" id="108271438"/>
    <dbReference type="KEGG" id="ipu:108271438"/>
    <dbReference type="CTD" id="114453"/>
    <dbReference type="GeneTree" id="ENSGT00940000167861"/>
    <dbReference type="OMA" id="QMDMISY"/>
    <dbReference type="OrthoDB" id="10032067at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030099">
        <property type="term" value="P:myeloid cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14693">
        <property type="entry name" value="bZIP_CEBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031106">
        <property type="entry name" value="C/EBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23334">
        <property type="entry name" value="CCAAT/ENHANCER BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23334:SF62">
        <property type="entry name" value="CCAAT_ENHANCER BINDING PROTEIN (C_EBP) 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="4">
        <location>
            <begin position="129"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="31"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="147"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="31"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017334532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017334541.1"/>
        </source>
    </evidence>
    <sequence length="207" mass="23362" checksum="72BEDEF3BD1F24B8" modified="2017-12-20" version="1">MSISHSRSTAHIFSSNNHSCSSLVSNTLALAPDSTTSSSSSLPSHISQMEEGPYGYSMGSQGLSRTSDRSGEPMMGMAYLPYSTCSNSTIERPAQQSHVIQQEFSQFLLPPPPSTFRQPGQKRGLSKDSMEYRLRRERNNIAVRKSRDKARRRIQLTQQKALELQEENHRLLLHIEQLSHEVDTLSRYLSQRHLQSKVNDLGVDENC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RY82</accession>
    <name>A0A2D0RY82_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="20 21 22">Protein kinase C and casein kinase substrate in neurons protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20 21 22 23" type="primary">pacsin1b</name>
    </gene>
    <organism evidence="19 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20 21 22" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20 21 22">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Ruffle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017335450.1">
        <property type="nucleotide sequence ID" value="XM_017479961.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017335451.1">
        <property type="nucleotide sequence ID" value="XM_017479962.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017335452.1">
        <property type="nucleotide sequence ID" value="XM_017479963.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017335453.1">
        <property type="nucleotide sequence ID" value="XM_017479964.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RY82"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000037147"/>
    <dbReference type="GeneID" id="108271966"/>
    <dbReference type="KEGG" id="ipu:108271966"/>
    <dbReference type="CTD" id="619246"/>
    <dbReference type="OMA" id="NCVNERA"/>
    <dbReference type="OrthoDB" id="10255128at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048812">
        <property type="term" value="P:neuron projection morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900006">
        <property type="term" value="P:positive regulation of dendrite development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030100">
        <property type="term" value="P:regulation of endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11998">
        <property type="entry name" value="SH3_PACSIN1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000014">
        <property type="entry name" value="Kinase C and casein kinase substrate in neurons protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000205">
        <property type="entry name" value="Protein kinase C and casein kinase substrate in neurons protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035743">
        <property type="entry name" value="PACSIN1/PACSIN2_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF16">
        <property type="entry name" value="PROTEIN KINASE C AND CASEIN KINASE SUBSTRATE IN NEURONS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8 14 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="20 21 22" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="20 21 22" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="F-BAR" evidence="18">
        <location>
            <begin position="12"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="384"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="337"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="188"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="337"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017335450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017335451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017335452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017335453.1"/>
        </source>
    </evidence>
    <sequence length="443" mass="51563" checksum="C42DBF7C8FE89E0A" modified="2017-12-20" version="1">MSVTYDESLVSDETTDSFWEVGNYKRTVKRIDDGHRLCNDLMSCIQDRAKIEKAYSQQLTDWAKRWRQLVERGPQYGSVERAWMAMMNETDKVSELHQDIKNMLINVDLEKVKNWQKDSYHKQIMGSFKETKEAEEGFRKAQKPWAKKLKEVETTKKAYHLACKEEKIASNREANNKGEASSTTAEQQKKFQEKLDKCRSEVQKAKEKYVKALDELSSCTPQYVENMELVFEQCQQFEEKRLAFLREVLLDIKRHLNLTENQSYASVYRELERTITSASPQEDLRWFSNNHGPGMHTNWPQFEEYNPELGHAINKKEKLKKNHDDVTLTYVMTVGDHQSSPQVDNRSSVSSYEKNQAYSAEWSDEEQAAAETNGGSNPFEEEKSQGVRVRALYDYEGQEQDELSFRVGEELTKLEDEDDQGWCKGRLDNGQLGLYPANYVEPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S1E8</accession>
    <name>A0A2D0S1E8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Retinoblastoma-like protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">107 kDa retinoblastoma-associated protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">pRb1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">rbl1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation. May act as a tumor suppressor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Interacts with AATF. Interacts with KDM5A. Interacts with KMT5B and KMT5C. Interacts with USP4. Interacts with RBBP9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the retinoblastoma protein (RB) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017336574.1">
        <property type="nucleotide sequence ID" value="XM_017481085.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S1E8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005452"/>
    <dbReference type="GeneID" id="108272598"/>
    <dbReference type="KEGG" id="ipu:108272598"/>
    <dbReference type="CTD" id="5933"/>
    <dbReference type="OrthoDB" id="844594at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000134">
        <property type="term" value="P:negative regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000035">
        <property type="entry name" value="RB transcriptional corepressor-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.140:FF:000001">
        <property type="entry name" value="Retinoblastoma-like 2, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000082">
        <property type="entry name" value="retinoblastoma-like protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002720">
        <property type="entry name" value="RB_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002719">
        <property type="entry name" value="RB_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015030">
        <property type="entry name" value="RB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028309">
        <property type="entry name" value="RB_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024599">
        <property type="entry name" value="RB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13742">
        <property type="entry name" value="RETINOBLASTOMA-ASSOCIATED PROTEIN RB -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13742:SF20">
        <property type="entry name" value="RETINOBLASTOMA-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11934">
        <property type="entry name" value="DUF3452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01858">
        <property type="entry name" value="RB_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01857">
        <property type="entry name" value="RB_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01367">
        <property type="entry name" value="DUF3452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01368">
        <property type="entry name" value="RB_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01369">
        <property type="entry name" value="Rb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Retinoblastoma-associated protein N-terminal" evidence="17">
        <location>
            <begin position="72"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Retinoblastoma-associated protein A-box" evidence="18">
        <location>
            <begin position="387"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="16">
        <location>
            <begin position="782"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="domain" description="Retinoblastoma-associated protein C-terminal" evidence="19">
        <location>
            <begin position="932"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="633"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="871"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1009"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1021"/>
            <end position="1031"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM01369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017336574.1"/>
        </source>
    </evidence>
    <sequence length="1048" mass="118686" checksum="AB2AA24D18F95376" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S1S5</accession>
    <name>A0A2D0S1S5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Endonuclease/exonuclease/phosphatase family domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">eepd1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017336699.1">
        <property type="nucleotide sequence ID" value="XM_017481210.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S1S5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027215"/>
    <dbReference type="GeneID" id="108272654"/>
    <dbReference type="KEGG" id="ipu:108272654"/>
    <dbReference type="CTD" id="80820"/>
    <dbReference type="OMA" id="HLVPANT"/>
    <dbReference type="OrthoDB" id="6237065at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10283">
        <property type="entry name" value="MnuA_DNase1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.320">
        <property type="entry name" value="Photosystem II 12 kDa extrinsic protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051675">
        <property type="entry name" value="Endo/Exo/Phosphatase_dom_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21180">
        <property type="entry name" value="ENDONUCLEASE/EXONUCLEASE/PHOSPHATASE FAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21180:SF32">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE FAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12836">
        <property type="entry name" value="HHH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="317"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="460"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="472"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017336699.1"/>
        </source>
    </evidence>
    <sequence length="483" mass="52277" checksum="B857D996B38501DF" modified="2017-12-20" version="1">MGGNLGCHRSLPADPADVRQRGRKFSATCSFAHISAHNPERLNVNAASEEELMTLPGVSRAIARAIVEHRARIGGFRKVEDVALVSGIGAAKLQLIKPEICVSSRRSNNNNNDPREQEHRASVAHSWTSSHPNSPAACTELHLPPGGPAHMASERPDVQPAPCARHGKPLLRVATWDLQRCSSDKANNPGVKEVVCRTLLERDIKLLAVQDLADQQALDKFCTELNQPTLSSVCNWKGPRGMWKCAASGKPTGESCIGSEYSGFLWDTSAGIQLKEAVVLESAAAANGNGNDAQPKAYLGHFRIGSSELTVVNVHVKPPSAPKQQNGRSHKSDELKSHRLSPGVQETLRGVKNVLVVGGFGLPPDSAQFEPLKKEKLSALVPSSVNTNISTKAPQGSSCLDNIWASRSIKKLYTGHCSVVREGLTNPWIPDNWSWGGVASEHCPIMAEFYTDYTNEKTTKEPVHNGSSVAVVERDDLTPKHER</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S2B8</accession>
    <name>A0A2D0S2B8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">[histone H3]-trimethyl-L-lysine(27) demethylase</fullName>
            <ecNumber evidence="13">1.14.11.68</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">kdm6al</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(27)-[histone H3] + 2 2-oxoglutarate + 2 O2 = N(6)-methyl-L-lysyl(27)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:60224"/>
            <dbReference type="Rhea" id="RHEA-COMP:15535"/>
            <dbReference type="Rhea" id="RHEA-COMP:15544"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="1.14.11.68"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the UTX family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.68" evidence="13"/>
    <dbReference type="RefSeq" id="XP_017336591.1">
        <property type="nucleotide sequence ID" value="XM_017481102.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000014154"/>
    <dbReference type="GeneID" id="108272609"/>
    <dbReference type="KEGG" id="ipu:108272609"/>
    <dbReference type="CTD" id="569207"/>
    <dbReference type="OrthoDB" id="418911at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044666">
        <property type="term" value="C:MLL3/4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071558">
        <property type="term" value="F:histone H3K27me2/H3K27me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000022">
        <property type="entry name" value="lysine-specific demethylase 6A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1370:FF:000001">
        <property type="entry name" value="lysine-specific demethylase 6A isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.20:FF:000001">
        <property type="entry name" value="lysine-specific demethylase 6A isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000002">
        <property type="entry name" value="lysine-specific demethylase 6A isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000011">
        <property type="entry name" value="lysine-specific demethylase 6A isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 6A"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="KDM6_C-hel"/>
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        <property type="entry name" value="HCP-like"/>
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            <end position="1230"/>
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            <end position="494"/>
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    <feature type="region of interest" description="Disordered" evidence="16">
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            <end position="627"/>
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            <end position="1048"/>
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        </location>
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            <begin position="891"/>
            <end position="904"/>
        </location>
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        <location>
            <begin position="1019"/>
            <end position="1033"/>
        </location>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00034525"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS51184"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017336591.1"/>
        </source>
    </evidence>
    <sequence length="1373" mass="151995" checksum="5364359E42188A33" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
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        <name evidence="7" type="primary">tmem235b</name>
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            <taxon>Siluriformes</taxon>
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            <taxon>Ictalurus</taxon>
        </lineage>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
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    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="7">Blood</tissue>
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        <property type="component" value="Chromosome 13"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017338419.1"/>
        </source>
    </evidence>
    <sequence length="200" mass="21860" checksum="18C76239C53EA335" modified="2017-12-20" version="1">MGLGFGAVVLGAGLSAILSFSFLALAIGTEYWYIIEDKRTNHTDPQHVHSGLWGVENDELPFTGDPSRFTESERQMQDLHYVIAVLLPLSLVLLVSGGICGMASSLAQSRLLLMGTASYFLLCSLLTLSGVSLYINYSQKALEEAEVRIGLEQMAMVHTSFGWSMGLAWLSFILEVITGLLLLLAAWMMQLTEQETVAPI</sequence>
</entry>
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    <accession>A0A2D0SDJ0</accession>
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        <submittedName>
            <fullName evidence="12">Uncharacterized protein LOC108274862</fullName>
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        <name evidence="12" type="primary">LOC108274862</name>
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    <organism evidence="11 12">
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            <taxon>Ictaluridae</taxon>
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    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017340792.1">
        <property type="nucleotide sequence ID" value="XM_017485303.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SDJ0"/>
    <dbReference type="GeneID" id="108274862"/>
    <dbReference type="KEGG" id="ipu:108274862"/>
    <dbReference type="OrthoDB" id="8960739at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF111">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012587459" evidence="9">
        <location>
            <begin position="28"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="19"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017340792.1"/>
        </source>
    </evidence>
    <sequence length="213" mass="23808" checksum="A26774F13F06E815" modified="2017-12-20" version="1">MAKLLSTFLLQMLFGFFTPCLLEIASTDHLSVPPGENITLLCNITNYYEISWYRLRSEEMKLLISAEKLKLKKTFFLRYNVNKSHFDITESSSSVSLVIIGVRETDLGFYFCGGRNNATHIQFGKLIRLDFTADQHHETDRSDTQQSVGPALCGIIIIILSCVCAVFVSISIICSSLYCSTLQDKCSSTGDSNEKEVDLHLSDLTSVTYTSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SEZ6</accession>
    <name>A0A2D0SEZ6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Krueppel-like factor 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">klf13</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the Sp1 C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017340820.1">
        <property type="nucleotide sequence ID" value="XM_017485331.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SEZ6"/>
    <dbReference type="GeneID" id="108274922"/>
    <dbReference type="KEGG" id="ipu:108274922"/>
    <dbReference type="CTD" id="51621"/>
    <dbReference type="OMA" id="KHRCHYA"/>
    <dbReference type="OrthoDB" id="6365676at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21571">
        <property type="entry name" value="KLF13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000595">
        <property type="entry name" value="Krueppel-like factor 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000018">
        <property type="entry name" value="Krueppel-like factor 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000232">
        <property type="entry name" value="Krueppel-like factor 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF21">
        <property type="entry name" value="KRUEPPEL-LIKE FACTOR 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="164"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="194"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="224"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="106"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="244"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="114"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="257"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="274"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00069428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017340820.1"/>
        </source>
    </evidence>
    <sequence length="298" mass="33155" checksum="15F5909D989DBF5E" modified="2017-12-20" version="1">MFSITQKGSTTELSDLDKCHFTFACNKETAGAFLFCVFDKMEHLAAECLVSMSSRAIVHGPKGNREIRPETAAAPRNGEETKEALVKDNSSLFVVARILADFNQQSPTNFAEQAKTKEESMPPTPTREDGNSATPTAVPTDSALKQRGKRWRARAEQDSPQKKHKCHYPGCEKVYGKSSHLKAHLRTHTGERPFPCTWPDCSKKFARSDELARHYRTHTGEKKFGCPLCDKRFMRSDHLMKHARRHSDFQPAMLKRQQGSNGTLNAVSSTRPGSLSDYSRSDASSPTLSPTLSPANSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SHN5</accession>
    <name>A0A2D0SHN5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Trichohyalin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC108275606</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017342001.3">
        <property type="nucleotide sequence ID" value="XM_017486512.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SHN5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000004806"/>
    <dbReference type="GeneID" id="108275606"/>
    <dbReference type="KEGG" id="ipu:108275606"/>
    <dbReference type="OrthoDB" id="62853at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="CDD" id="cd05834">
        <property type="entry name" value="PWWP_HRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SLE9</accession>
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        <recommendedName>
            <fullName evidence="3">High mobility group nucleosome-binding domain-containing protein 3</fullName>
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        <name evidence="8" type="primary">hmgn3</name>
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        <name type="scientific">Ictalurus punctatus</name>
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            <taxon>Teleostei</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HMGN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017343533.1">
        <property type="nucleotide sequence ID" value="XM_017488044.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SLE9"/>
    <dbReference type="GeneID" id="108276400"/>
    <dbReference type="KEGG" id="ipu:108276400"/>
    <dbReference type="CTD" id="9324"/>
    <dbReference type="OrthoDB" id="8956258at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031492">
        <property type="term" value="F:nucleosomal DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000079">
        <property type="entry name" value="HMGN_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23087:SF2">
        <property type="entry name" value="HIGH MOBILITY GROUP NUCLEOSOME-BINDING DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23087">
        <property type="entry name" value="NONHISTONE CHROMOSOMAL PROTEIN HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01101">
        <property type="entry name" value="HMG14_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00925">
        <property type="entry name" value="NONHISHMG17"/>
    </dbReference>
    <dbReference type="SMART" id="SM00527">
        <property type="entry name" value="HMG17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017343533.1"/>
        </source>
    </evidence>
    <sequence length="96" mass="10187" checksum="B7375AB3DB468419" modified="2017-12-20" version="1">MPKRKSPEGAEGKDSTKVTKQEPTRRSERLSSKPAPPKTEPKPKKAAAKKASDDKAAKGKKGGAKGKKEEKEAVPTENGETKAEGESTEAEADAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SMM5</accession>
    <name>A0A2D0SMM5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Protocadherin alpha-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC108276665</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017343959.1">
        <property type="nucleotide sequence ID" value="XM_017488470.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SMM5"/>
    <dbReference type="GeneID" id="108276665"/>
    <dbReference type="KEGG" id="ipu:108276665"/>
    <dbReference type="OrthoDB" id="6252479at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF32">
        <property type="entry name" value="CADHERIN-RELATED NEURONAL RECEPTOR VARIABLE 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013402101" evidence="14">
        <location>
            <begin position="27"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="682"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="31"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="131"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="235"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="343"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="448"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="570"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017343959.1"/>
        </source>
    </evidence>
    <sequence length="792" mass="86510" checksum="A0558C32CDF1A1AD" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SMP5</accession>
    <name>A0A2D0SMP5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Class A basic helix-loop-helix protein 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">bhlha9</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017343977.1">
        <property type="nucleotide sequence ID" value="XM_017488488.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SMP5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000031297"/>
    <dbReference type="GeneID" id="108276681"/>
    <dbReference type="KEGG" id="ipu:108276681"/>
    <dbReference type="CTD" id="727857"/>
    <dbReference type="OrthoDB" id="6241467at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070888">
        <property type="term" value="F:E-box binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18912">
        <property type="entry name" value="bHLH_TS_bHLHa9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050359">
        <property type="entry name" value="bHLH_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="4">
        <location>
            <begin position="70"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="38"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017343977.1"/>
        </source>
    </evidence>
    <sequence length="282" mass="31433" checksum="74E18DB787A974A4" modified="2017-12-20" version="1">MTSRSSFTLHSGSEFSEEELELSPEAQMDDDTLDSSRKGSYTDSASPSSTLSEAEEGRPKKRSRPVRSTARRMAANVRERKRILDYNQAFNALRVALHHDLSGKRLSKIATLQRAINRISALSVFLRNNPPNGASKPCTHRECYGQPGQVSPTDVEPQGFMSWEQPLALQMQKHRLPSEQQVYMDHFRHAGSTCPLLPHYSCYSPDVQLYANGGSCGSPASDDASPPRYGRQGDWLGHQAGVWGSCSQRYADGHSEATQTLPFSWHMGYLQETGPQHCYPML</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SRA1</accession>
    <name>A0A2D0SRA1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ATP synthase subunit C lysine N-methyltransferase isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC108277217</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ANT/ATPSC lysine N-methyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017345232.2">
        <property type="nucleotide sequence ID" value="XM_017489743.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SRA1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022591"/>
    <dbReference type="GeneID" id="108277217"/>
    <dbReference type="KEGG" id="ipu:108277217"/>
    <dbReference type="OrthoDB" id="66144at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905706">
        <property type="term" value="P:regulation of mitochondrial ATP synthesis coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026170">
        <property type="entry name" value="FAM173A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13610">
        <property type="entry name" value="METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13610:SF18">
        <property type="entry name" value="SI:DKEY-190G11.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017345232.2"/>
        </source>
    </evidence>
    <sequence length="286" mass="32593" checksum="7B10A89BEE6A7507" modified="2023-06-28" version="2">MRRDACWRCEPCEERPRSRSPLLLLLQARENAHRRAHAWHATERVRDYRLRHFLRPQHAGSFLESGASMEDSIEVMLQERNSKLLYHYRNNRVLTFAAGAIFSGLYGLWAMFCLPGLKVPLRLKVPFLPSTKVQTENVMKLLEGRQGWLADLGAGDGRLVFAACSAGFQCTGFEINSMLLAYSRGQAWWKGIPHTHATFVKQDFWKTDLSKYTNVVAFLAPAVMADLEKKLLKELPVDARVVVCSFPFPHWPHSCTIGSGLDQVWAYNVSSVRETRDATSSFRTHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SV52</accession>
    <name>A0A2D0SV52_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Spartin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">spartb</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Lipophagy receptor that plays an important role in lipid droplet (LD) turnover in motor neurons. Localizes to LDs and interacts with components of the autophagy machinery, such as MAP1LC3A/C proteins to deliver LDs to autophagosomes for degradation via lipophagy. Lipid transfer protein required for lipid droplet degradation, including by lipophagy. Can bind and transfer all lipid species found in lipid droplets, from phospholipids to triglycerides and sterol esters but the direction of lipid transfer by spartin and its cargos are unknown. May be implicated in endosomal trafficking, or microtubule dynamics, or both. Participates in cytokinesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with ITCH and WWP1. Interacts (via MIT domain) with IST1; leading to the recruitment of SPART to midbodies. Interacts with MAP1LC3A and MAP1LC3C.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017346558.1">
        <property type="nucleotide sequence ID" value="XM_017491069.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SV52"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020042"/>
    <dbReference type="GeneID" id="108277982"/>
    <dbReference type="KEGG" id="ipu:108277982"/>
    <dbReference type="CTD" id="751741"/>
    <dbReference type="OMA" id="WQGMECA"/>
    <dbReference type="OrthoDB" id="20821at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:midbody"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:cell division"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:lipophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="MIT_spastin"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="spartin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MIT_dom"/>
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        <property type="entry name" value="Senescence/spartin_C"/>
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        <property type="entry name" value="Spartin-like"/>
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        <property type="entry name" value="SPARTIN"/>
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        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Senescence"/>
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        <property type="entry name" value="MIT"/>
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    </dbReference>
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        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
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    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
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    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="8" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="14" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="MIT" evidence="19">
        <location>
            <begin position="17"/>
            <end position="95"/>
        </location>
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        <location>
            <begin position="100"/>
            <end position="155"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="559"/>
            <end position="579"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00054810"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00064034"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00067916"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00745"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017346558.1"/>
        </source>
    </evidence>
    <sequence length="579" mass="62716" checksum="5B67F1426A3B0EBF" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SWW0</accession>
    <name>A0A2D0SWW0_ICTPU</name>
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        <submittedName>
            <fullName evidence="6">Uncharacterized protein LOC108278357 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC108278357</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017347174.1">
        <property type="nucleotide sequence ID" value="XM_017491685.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SWW0"/>
    <dbReference type="GeneID" id="108278357"/>
    <dbReference type="KEGG" id="ipu:108278357"/>
    <dbReference type="OrthoDB" id="8963697at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013356712" evidence="3">
        <location>
            <begin position="23"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="18"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="342"/>
        </location>
    </feature>
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        <location>
            <begin position="266"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017347174.1"/>
        </source>
    </evidence>
    <sequence length="342" mass="39141" checksum="289F767A381CE932" modified="2017-12-20" version="1">MCVKYFVQLLTFFSALVPYGLNCSESCSNTTLNIWLGSEVLLPCSLLKSGKTEEVRWSQTSSLLSIGSSGNVTFEDPRDGRLTAFPFLFGRGNFSILVHQFQASDLGIYCCQLSHECQRVEIKLSQSLESHKGGLNFPWYYIVAGVGIFILLVTVFCLIYKFRAGKCLNSASDSYYVNQEQREGEHRDRVDRTKVDECDGEYEDIESDTQGADYENASGEDTMREEQDEDYVNTERRNGHDQYYDTDRECPAEHHVERDMQGYHDNDVYENDEHNPKQKSKVPPKAAAAFRKMPNQQPGSVGLYYANQSEIDKSRCARNRNKDKAEYQFKNPLYDRTPANNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SYH3</accession>
    <name>A0A2D0SYH3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ADP-ribosylation factor-like protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">arl3l1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347581.1">
        <property type="nucleotide sequence ID" value="XM_017492092.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SYH3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029296"/>
    <dbReference type="GeneID" id="108278630"/>
    <dbReference type="KEGG" id="ipu:108278630"/>
    <dbReference type="CTD" id="393666"/>
    <dbReference type="OMA" id="GMNWICS"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04155">
        <property type="entry name" value="Arl3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000281">
        <property type="entry name" value="ADP-ribosylation factor-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044612">
        <property type="entry name" value="ARL2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45697">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="6 12 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="29"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="131"/>
            <end position="134"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017347581.1"/>
        </source>
    </evidence>
    <sequence length="187" mass="21101" checksum="CDEC85C242CAE708" modified="2017-12-20" version="1">MGEIQKGLFSVIEKLKGTTEQELRIVLLGLDNAGKTTLLKKLASEDVNMITPTQGFNIKSVTFHGMKLNVWDIGGQRKIRPFWKKYLENTDLLIYVIDSADKKRFEETGLELSELIDEENLKGVPLLIFANKQDLTTASTASEITEGLNLHTYRDREWQIQACSAISGEGVQDGMNWISNNIVKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SYK7</accession>
    <name>A0A2D0SYK7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Homeobox protein Hox-B2a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">hoxb2a</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Antp homeobox family. Proboscipedia subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347420.1">
        <property type="nucleotide sequence ID" value="XM_017491931.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SYK7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003401"/>
    <dbReference type="GeneID" id="108278501"/>
    <dbReference type="KEGG" id="ipu:108278501"/>
    <dbReference type="CTD" id="30338"/>
    <dbReference type="OMA" id="FTEDACL"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000145">
        <property type="entry name" value="homeobox protein Hox-A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45664:SF7">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45664">
        <property type="entry name" value="PROTEIN ZERKNUELLT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="177"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="179"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="67"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="232"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="295"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017347420.1"/>
        </source>
    </evidence>
    <sequence length="416" mass="45091" checksum="540B080FD3B14F0B" modified="2017-12-20" version="1">MLFLRIFYVAGRPFCLGKAMNFEFEREIGFINSQPSLAECLTSFPAVLESFQTSSIKDSTVIPPPFEHTIPSLSPCTGNQARPRTHKRTASNGLQLRTQTTPPTQQKQGPAPATAGGPLAHEFPWMKEKKSSKKCPKPGAALTAATAAASPSPASSGFTTAGLESPTELQVGLDNGSGSRRLRTAYTNTQLLELEKEFHFNKYLCRPRRVEIAALLDLTERQVKVWFQNRRMKHKRQTTHHRDGQEGESSGFEPLEGSDAASPFPNPPPEPSGPTASSSSSASASESEPCLPASCYSNSADNTHPTPEESQPIQTESADGPDTAVHSPPYPTPTADNPGTMAESREELPENAFTQPQDANSLADLNFFSADSCLQISDALSPSLQSSLDSPVDFSEEDFDLFTSTLCTIDLQHLQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T649</accession>
    <name>A0A2D0T649_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transmembrane protein 178B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tmem178bb</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM178 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017350377.1">
        <property type="nucleotide sequence ID" value="XM_017494888.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T649"/>
    <dbReference type="GeneID" id="108280127"/>
    <dbReference type="KEGG" id="ipu:108280127"/>
    <dbReference type="CTD" id="436669"/>
    <dbReference type="OMA" id="RQHNSTN"/>
    <dbReference type="OrthoDB" id="8809386at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039625">
        <property type="entry name" value="T178A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32005:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 178B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32005">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 178B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13903">
        <property type="entry name" value="Claudin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012903743" evidence="8">
        <location>
            <begin position="29"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="248"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017350377.1"/>
        </source>
    </evidence>
    <sequence length="294" mass="33226" checksum="B21492BA6F0C498D" modified="2017-12-20" version="1">MAAGKLLLYAGLSLSLCALGMLAVAVCSDHWYETDARRYRERCRSFSSRRKDPGFIYIPNNSLPLRASRSRLDRWEGKLLVTRNRRQLFAMSAADECSRQYNSTNMGLWSKCHRLGFDQDIEDLIRKGSIARCSYIKYHYSSATIPKNLSYNITKTIRQDEWHSLHLRRMTAGFMGMAVAIILFGWIIGMLGCCWDRGLMQYVAGLLFLMGGTFCIISLCTCVAGINFELSRYPRYLYGLPDDISHGYGWSMFCAWGGLGLSLIAGFFCTLAPSVQPIPRSTCPKSRQENGTVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T6Z3</accession>
    <name>A0A2D0T6Z3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zinc finger FYVE domain-containing protein 9 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">zfyve9a</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017350657.1">
        <property type="nucleotide sequence ID" value="XM_017495168.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T6Z3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021469"/>
    <dbReference type="GeneID" id="108280307"/>
    <dbReference type="KEGG" id="ipu:108280307"/>
    <dbReference type="CTD" id="100005449"/>
    <dbReference type="OrthoDB" id="5872154at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15729">
        <property type="entry name" value="FYVE_endofin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.220:FF:000001">
        <property type="entry name" value="Zinc finger, FYVE domain-containing 9a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.500.40:FF:000001">
        <property type="entry name" value="Zinc finger, FYVE domain-containing 9a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.720.10:FF:000001">
        <property type="entry name" value="Zinc finger, FYVE domain-containing 9a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000084">
        <property type="entry name" value="Zinc finger, FYVE domain-containing 9b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.220">
        <property type="entry name" value="Domain of unknown function (DUF3480), N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.720.10">
        <property type="entry name" value="Smad anchor for receptor activation, Smad-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022557">
        <property type="entry name" value="SARA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024608">
        <property type="entry name" value="SARA-like_SBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035438">
        <property type="entry name" value="SARA/endofin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037145">
        <property type="entry name" value="SARA_Smad-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46319">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46319:SF2">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11409">
        <property type="entry name" value="SARA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11979">
        <property type="entry name" value="SARA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037289">
        <property type="entry name" value="SARA/endofin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01421">
        <property type="entry name" value="DUF3480"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01422">
        <property type="entry name" value="SARA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="12">
        <location>
            <begin position="481"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="146"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="267"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="417"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="545"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="616"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="280"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="353"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="547"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="630"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="643"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017350657.1"/>
        </source>
    </evidence>
    <sequence length="1203" mass="131319" checksum="9D5CA8B4808C365C" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979E627</accession>
    <name>A0A979E627_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">tspan33b</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047008747.2">
        <property type="nucleotide sequence ID" value="XM_047152791.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979E627"/>
    <dbReference type="GeneID" id="124626782"/>
    <dbReference type="KEGG" id="ipu:124626782"/>
    <dbReference type="CTD" id="567512"/>
    <dbReference type="OrthoDB" id="2014092at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046931">
        <property type="term" value="P:pore complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03158">
        <property type="entry name" value="penumbra_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000007">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF397">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="57"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="154"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="155"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_047008747.2"/>
        </source>
    </evidence>
    <sequence length="270" mass="30631" checksum="F2C225473A80D64F" modified="2023-06-28" version="2">MDRDLNLSRAETFSVINPCIRYFLFLFSFLFWVFSLCIVAIGVYAKVQKATDPVRDTFLVDPAIILIVVGVVMFFITYCGCIGALRENIQLLKTFSFSLTLVFLTQLIITILVFFYSEQTRDAMGKFVKKAIVHYRDDLDLQNLLDYVQQEFRCCGWNNYTDWSWNVYFNCSQRNPSKERCAVPFSCCTPVPRETVINTMCGFGVQTQKNSEAAESIYTEGCADRAVTWIESHLLLVGSLALGLALPQIAGIVLSHILVSQVQNEINSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979ECA2</accession>
    <name>A0A979ECA2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein LOC124626324</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC124626324</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_047007084.1">
        <property type="nucleotide sequence ID" value="XM_047151128.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979ECA2"/>
    <dbReference type="GeneID" id="124626324"/>
    <dbReference type="KEGG" id="ipu:124626324"/>
    <dbReference type="OrthoDB" id="8930837at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042923">
        <property type="term" value="F:neuropeptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004994">
        <property type="term" value="F:somatostatin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071392">
        <property type="term" value="P:cellular response to estradiol stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071385">
        <property type="term" value="P:cellular response to glucocorticoid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229">
        <property type="entry name" value="NEUROPEPTIDES RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229:SF6">
        <property type="entry name" value="SOMATOSTATIN RECEPTOR TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="182"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="22"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_047007084.1"/>
        </source>
    </evidence>
    <sequence length="304" mass="34334" checksum="621CB6A90CB23F2F" modified="2023-02-22" version="1">MEQSVFPLFVLRIIISVIGIVGNGVLIISILHLTRVKTFEIFLLGLAITNFAEIMLVDIYDAIVQSIQTVSILSCVALKFLNICGENASIFFTVLISFYRYQKIHNAAMRIIAPIFMDSREAAIGLSMGCILVAVLLGLPTYFINQDTWHMENSTTEVCPADFFQCSEVHCPTVNSIYKNLFITICHVLPLIIVTWTSVLIIKILIIQQKAVDALHESDPGAVAVHHHHEHDVFHRSTIGILAAMTLFQVDCILYLILHFVYNPYDFPAWSELEFFITTLYTGIIPYIYGTGHNFFSVKHFMKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EHV6</accession>
    <name>A0A979EHV6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">pak5</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_047007069.1">
        <property type="nucleotide sequence ID" value="XM_047151113.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EHV6"/>
    <dbReference type="GeneID" id="108257526"/>
    <dbReference type="CTD" id="57144"/>
    <dbReference type="OrthoDB" id="1022360at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01093">
        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06658">
        <property type="entry name" value="STKc_PAK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000073">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000141">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000002">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028754">
        <property type="entry name" value="STKc_PAK5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF4">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="14">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="489"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="81"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="123"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="259"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="309"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="366"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="412"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="519"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_047007069.1"/>
        </source>
    </evidence>
    <sequence length="759" mass="85114" checksum="B86B92EB92C4B59B" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EMY2</accession>
    <name>A0A979EMY2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 4E-1B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">eif4e1b</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047007751.1">
        <property type="nucleotide sequence ID" value="XM_047151795.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EMY2"/>
    <dbReference type="GeneID" id="108260188"/>
    <dbReference type="CTD" id="253314"/>
    <dbReference type="OrthoDB" id="590761at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.760.10:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019770">
        <property type="entry name" value="TIF_eIF_4E_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF75">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00813">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_047007751.1"/>
        </source>
    </evidence>
    <sequence length="226" mass="25828" checksum="65597196498B4A19" modified="2023-02-22" version="1">MPPDDVTEGVTADQSAEQIDKRPKKVVEKKETAVVVLVRKELHAKHALQNRWALWFYKNDKSKMWQDNLRLITKFDTVEDFWGLYHNIQMASKLSSGCDYSVFKDGIEPMWEDRSNKCGGRWLITLSKQQRHTELDRFWLETLLCLIGEGFGPYSRDVCGAVINIRAKGDKIAVWTTNTENAEAVTYIGRKYKESLGLPVKLVIGYQAHADTATKSNSIAKNKFVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EVN9</accession>
    <name>A0A979EVN9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Nyctalopin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nyx</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047011852.2">
        <property type="nucleotide sequence ID" value="XM_047155896.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EVN9"/>
    <dbReference type="GeneID" id="108266365"/>
    <dbReference type="KEGG" id="ipu:108266365"/>
    <dbReference type="CTD" id="60506"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000172">
        <property type="entry name" value="Nyctalopin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF210">
        <property type="entry name" value="CHAOPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="LRRCT" evidence="4">
        <location>
            <begin position="337"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_047011852.2"/>
        </source>
    </evidence>
    <sequence length="466" mass="52932" checksum="36AEB09D97FD3C09" modified="2023-06-28" version="2">MTPPPSTGPEGSLNVWVMVLPHGMTLWACSSSCPPSCMCTQEKSCTVLCDRANMAELPPEFPCEASSINLEKNSLKFLSERAFGTLPVLKSLFLDHNNISFITPGAFRGLPNLLELTMAHNEYIRYLHTRTFTSLKHLLRLDLSGCNLFNLPDRIFLEQTSLRELFCFQNNFRRIPIAIRGMENLTHVYLERNKIEAVAYNTLLGLCNLKYLNLQENRISVIHDEAFKDLRQLQNLYLNDNLLVDLPRRSFKGLCNLKMLNLGGNLLTNISNTWFGDLVELEVLYLDRNCLSFIDKGAFENLTSLMTLHLNSNKLTSLPFSIFQPIYFIGHLYLFRNPWECSCELAWLKKWMENYKLVRDIPCASPPSVAGLDLSKVLYAHVNGTCIDPAEFNLTTAVPDYSTTQRKFNSLISTLMNQEFREVERNFTEGQRNGTVLEPAEGQVSAAHRPSCVGLVLLLLLPHIQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F726</accession>
    <name>A0A979F726_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Protocadherin beta-6 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC108275059</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_047015709.1">
        <property type="nucleotide sequence ID" value="XM_047159753.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979F726"/>
    <dbReference type="GeneID" id="108275059"/>
    <dbReference type="OrthoDB" id="6252479at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000018">
        <property type="entry name" value="Protocadherin gamma c3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF288">
        <property type="entry name" value="PROTOCADHERIN ALPHA-C2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="765"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="98"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="207"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="316"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="424"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="529"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="653"/>
            <end position="756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_047015709.1"/>
        </source>
    </evidence>
    <sequence length="877" mass="98119" checksum="88654939648C529D" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QTK5</accession>
    <name>A0A9F7QTK5_ICTPU</name>
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        <submittedName>
            <fullName evidence="12">Uncharacterized protein LOC108280624 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC108280624</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Inflammasome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053529839.1">
        <property type="nucleotide sequence ID" value="XM_053673864.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108280624"/>
    <dbReference type="KEGG" id="ipu:108280624"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050637">
        <property type="entry name" value="NLRP_innate_immun_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45690">
        <property type="entry name" value="NACHT, LRR AND PYD DOMAINS-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45690:SF19">
        <property type="entry name" value="NACHT, LRR AND PYD DOMAINS-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1271">Inflammasome</keyword>
    <keyword evidence="7" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
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        <location>
            <begin position="159"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1139"/>
            <end position="1181"/>
        </location>
    </feature>
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        <location>
            <begin position="1141"/>
            <end position="1153"/>
        </location>
    </feature>
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        <location>
            <begin position="1166"/>
            <end position="1181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053529839.1"/>
        </source>
    </evidence>
    <sequence length="1564" mass="178026" checksum="5447DB4660415ED5" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R2M5</accession>
    <name>A0A9F7R2M5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4 5">Uncharacterized protein LOC108262077 isoform X8</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="7 8 9">Uncharacterized protein LOC124627608</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7 8 9" type="primary">LOC124627608</name>
        <name evidence="3 4 5 6" type="synonym">LOC108262077</name>
    </gene>
    <organism evidence="2 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3 4 5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3 4 5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053532172.1">
        <property type="nucleotide sequence ID" value="XM_053676197.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053532173.1">
        <property type="nucleotide sequence ID" value="XM_053676198.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053532174.1">
        <property type="nucleotide sequence ID" value="XM_053676199.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053532175.1">
        <property type="nucleotide sequence ID" value="XM_053676200.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053532192.1">
        <property type="nucleotide sequence ID" value="XM_053676217.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053532193.1">
        <property type="nucleotide sequence ID" value="XM_053676218.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053532194.1">
        <property type="nucleotide sequence ID" value="XM_053676219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R2M5"/>
    <dbReference type="GeneID" id="124627608"/>
    <dbReference type="KEGG" id="ipu:124627608"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053532172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053532173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053532174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_053532175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053532192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053532193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_053532194.1"/>
        </source>
    </evidence>
    <sequence length="254" mass="28505" checksum="5E972B876889C773" modified="2023-06-28" version="1">METLPERPTPEPRPRSMLTQEEWSRVDATATLRRPRTPHDSSSERVQLNETSTVRRRPKVIAPTQRDDATDAAIRVRPVSEVEHVYRESDMSEDFRRALKPPVSPKPSLGLRKPEPPTPTRRVPLPGPENQHSTVEGKRVPPPVSPKPSPPPTLPKPIKMKPLPTHTLTPPSDLNTHPHSSTILPEQVDHPILSPSTPTPQTSRISHFVPMTFDISRSEPPNPPNSFYPWVRICPSQTPEIFHGRAVSGHSQPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R5K2</accession>
    <name>A0A9F7R5K2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Polyadenylate-binding protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC108255406</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="6">SDA103</strain>
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            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="7">Blood</tissue>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
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                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
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                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
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                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
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                <person name="Yuan Z."/>
                <person name="Yang Y."/>
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                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11">SDA103</strain>
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        <property type="entry name" value="Znf_RING_CS"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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        <source>
            <dbReference type="PROSITE" id="PS50089"/>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50188"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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        <source>
            <dbReference type="RefSeq" id="XP_053537625.1"/>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="10">Blood</tissue>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
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        <property type="nucleotide sequence ID" value="XM_053678366.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RCZ1"/>
    <dbReference type="GeneID" id="128629619"/>
    <dbReference type="KEGG" id="ipu:128629619"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000210">
        <property type="entry name" value="Si:dkey-16p6.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="8">
        <location>
            <begin position="191"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053534341.1"/>
        </source>
    </evidence>
    <sequence length="694" mass="79539" checksum="51E5F64A83D1DF5B" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RDL1</accession>
    <name>A0A9F7RDL1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="1">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108262214</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="1"/>
    <dbReference type="RefSeq" id="XP_053534493.1">
        <property type="nucleotide sequence ID" value="XM_053678518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RDL1"/>
    <dbReference type="GeneID" id="108262214"/>
    <dbReference type="KEGG" id="ipu:108262214"/>
    <dbReference type="OrthoDB" id="26387at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF28">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053534493.1"/>
        </source>
    </evidence>
    <sequence length="280" mass="31477" checksum="C4B13333FE0092F5" modified="2023-06-28" version="1">MRPLVDGKKLFLWREVFGLDGPQPPPRGECLEKFVSSVRGVSHNRDCPLQGPVQVMKGWQIAANHLLSRANDMLQSVLVLGSGSSVLDDLNRSPSSCDGALVCVGPRRWRARGGERRQMEMCILCHEVQEILPDGAAMVLAAFVQRSPVMSKNRKRPPHNPKSYDPLFMHPDLSFGTHTGSCGHIMHSHCWQRYFETLQNQDAQWLHEQSSYDVENGEYLCPLCKCRSNTVIPLLPLTETTCSYSNSEQPGLAHWLNTTCQQIRGLHSAHKRAKQQVSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RE88</accession>
    <name>A0A9F7RE88_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14 15">Octopamine receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">LOC128629458</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053533633.1">
        <property type="nucleotide sequence ID" value="XM_053677658.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053533634.1">
        <property type="nucleotide sequence ID" value="XM_053677659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RE88"/>
    <dbReference type="GeneID" id="128629458"/>
    <dbReference type="KEGG" id="ipu:128629458"/>
    <dbReference type="OrthoDB" id="10071887at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000497">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752:SF13">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="41"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="78"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="343"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="384"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="57"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="431"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_053533633.1"/>
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        <source>
            <dbReference type="RefSeq" id="XP_053533634.1"/>
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    <sequence length="559" mass="61919" checksum="FE3C16B40F91D0E7" modified="2023-06-28" version="1"> </sequence>
</entry>
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        <scope>IDENTIFICATION</scope>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
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    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF140996">
        <property type="entry name" value="Hermes dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50808">
        <property type="entry name" value="ZF_BED"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 6" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="66"/>
            <end position="121"/>
        </location>
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        </location>
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            <end position="153"/>
        </location>
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    </evidence>
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            <dbReference type="RefSeq" id="XP_053540945.1"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RQ43</accession>
    <name>A0A9F7RQ43_ICTPU</name>
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    </protein>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
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    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053541273.1">
        <property type="nucleotide sequence ID" value="XM_053685298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RQ43"/>
    <dbReference type="GeneID" id="128634604"/>
    <dbReference type="KEGG" id="ipu:128634604"/>
    <dbReference type="OrthoDB" id="8836910at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042130">
        <property type="term" value="P:negative regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042102">
        <property type="term" value="P:positive regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031295">
        <property type="term" value="P:T cell costimulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051713">
        <property type="entry name" value="T-cell_Activation_Regulation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466:SF14">
        <property type="entry name" value="BUTYROPHILIN SUBFAMILY 2 MEMBER A2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466">
        <property type="entry name" value="T-LYMPHOCYTE ACTIVATION ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039947004" evidence="11">
        <location>
            <begin position="22"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="26"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="103"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_053541273.1"/>
        </source>
    </evidence>
    <sequence length="253" mass="27875" checksum="AAA2E1FBE3E3D747" modified="2023-06-28" version="1">MKSVLMSVYLLLLQCHPFIVREIIVNEPVTFLCTCSGPCPVGRWTRFIPGNTVIAEGRMCHSEQRYQKRFAVPGDQSRGDFSLKISSVTYNDAGSYRCSCNGESVTEVKLKVIVPTGVKAFEGENVILPCYGDTRRDVKDVKWKKDGQKVLLYTHANRSVTTDEASESRLMMSVEGFLDGDLSLHIGSVRLSDAGVYQCLIHDESQDGEPRAVLLKVEGLQELTTTNSAEVTVRPVLLGLIIAVGVIGLTFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TCC3</accession>
    <name>A0A9F7TCC3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">GTPase IMAP family member 4-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC128629146</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053531674.1">
        <property type="nucleotide sequence ID" value="XM_053675699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TCC3"/>
    <dbReference type="GeneID" id="128629146"/>
    <dbReference type="KEGG" id="ipu:128629146"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003360">
        <property type="entry name" value="US22-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF62">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 4-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02393">
        <property type="entry name" value="US22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="4">
        <location>
            <begin position="6"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_053531674.1"/>
        </source>
    </evidence>
    <sequence length="430" mass="49797" checksum="D10C67EEC018C6FD" modified="2023-06-28" version="1">MATVEVPELRIVILGKSFPQTTTVGNFILGRSAFETEDRPHSVERHCEMVGGHVEGRYITIINTPHLYNPKLSNEELNRKIKECVSRSRPGPHAFLLVVQPHDFTQKDRNLLRYILNSFGDQAINYSIVINTDSGFNTSEDELRFTAIQELTEECHGRHHSFSQLHESSRSSVCQLFEKIEKSFKPDDLTAHQKQDERAVQGLGVRLFSQYLHVESRSSSSTMSKQDKIEKDFLLPDLKKDGVKLYLQKIRKVASDNRGSEIKLKKVEEYSFKVGTLEETSYKDEPDMVDEWEQFYLPEHMFMEVIGVFEEFPCCSPNGDLVLMVCEDEKVFAFEDETLHLVSNNLEELFKNGLQFPGTEEYYRGQTFEDMTEEDWDRVRESDGLKEKRKEYQELLESMKDSFLNNLNLIMGRKRVVPIPDPEEKPSVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TGH0</accession>
    <name>A0A9F7TGH0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Apoptosis regulator BAX</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC128629193</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion outer membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Bcl-2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053532069.1">
        <property type="nucleotide sequence ID" value="XM_053676094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TGH0"/>
    <dbReference type="GeneID" id="128629193"/>
    <dbReference type="KEGG" id="ipu:128629193"/>
    <dbReference type="OrthoDB" id="6080198at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051400">
        <property type="term" value="F:BH domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097192">
        <property type="term" value="P:extrinsic apoptotic signaling pathway in absence of ligand"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008630">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008053">
        <property type="term" value="P:mitochondrial fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001836">
        <property type="term" value="P:release of cytochrome c from mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06845">
        <property type="entry name" value="Bcl-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.437.10:FF:000004">
        <property type="entry name" value="apoptosis regulator BAX isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.437.10">
        <property type="entry name" value="Blc2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036834">
        <property type="entry name" value="Bcl-2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046371">
        <property type="entry name" value="Bcl-2_BH1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026298">
        <property type="entry name" value="Bcl-2_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002475">
        <property type="entry name" value="Bcl2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256:SF42">
        <property type="entry name" value="APOPTOSIS REGULATOR BAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256">
        <property type="entry name" value="BCL-2 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00452">
        <property type="entry name" value="Bcl-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01862">
        <property type="entry name" value="BCL2FAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00337">
        <property type="entry name" value="BCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56854">
        <property type="entry name" value="Bcl-2 inhibitors of programmed cell death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50062">
        <property type="entry name" value="BCL2_FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Bcl-2 Bcl-2 homology region 1-3" evidence="18">
        <location>
            <begin position="64"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00067217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_053532069.1"/>
        </source>
    </evidence>
    <sequence length="191" mass="21439" checksum="25A4309BD5FC4E25" modified="2023-06-28" version="1">MAAALSGEGDAGPDQILEVGAVLLKDFIYERVHRHGDSGTVVSRHELGGSELCDPTHKKLAQCLQQIGDELDNNVDLQRMLADSALQPTKEVFVKVAREIFSDGKFNWGRVVALFYFACRLVIKALLTKIPDIIRTIINWTLDYLREHVINWIREQGGWEGIRSYFGTPTWQTVGVFLAGVLTTVLVIRKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TIW4</accession>
    <name>A0A9F7TIW4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="2 3">Uncharacterized protein LOC108262456 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2 3" type="primary">LOC108262456</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2 3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053534902.1">
        <property type="nucleotide sequence ID" value="XM_053678927.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053534903.1">
        <property type="nucleotide sequence ID" value="XM_053678928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TIW4"/>
    <dbReference type="GeneID" id="108262456"/>
    <dbReference type="KEGG" id="ipu:108262456"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_053534902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053534903.1"/>
        </source>
    </evidence>
    <sequence length="160" mass="18248" checksum="94C4E113B46DB4C8" modified="2023-06-28" version="1">MIECFNQIAEDSKCRVVVFSGAGKIFTAVYFGYRQRKLSESLRDRMERTQPCLHSAHCLPAWETFCSVTCCLACWELCCSGLWSPVEDTGLSPCCRGWWLALKRCVSAQLLHLPVKEQGTVTRRLCGTKTKQREKGRLNCAGKRPWLKHYRARVILSIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TJN4</accession>
    <name>A0A9F7TJN4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">asap1a</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053537783.1">
        <property type="nucleotide sequence ID" value="XM_053681808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TJN4"/>
    <dbReference type="GeneID" id="108267580"/>
    <dbReference type="CTD" id="100170829"/>
    <dbReference type="OrthoDB" id="435430at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08848">
        <property type="entry name" value="ArfGap_ASAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13251">
        <property type="entry name" value="PH_ASAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11965">
        <property type="entry name" value="SH3_ASAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000002">
        <property type="entry name" value="arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000004">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.950:FF:000001">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000006">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000012">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000012">
        <property type="entry name" value="Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043593">
        <property type="entry name" value="ASAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038016">
        <property type="entry name" value="ASAP1_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037844">
        <property type="entry name" value="PH_ASAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45854:SF2">
        <property type="entry name" value="ARF-GAP WITH SH3 DOMAIN, ANK REPEAT AND PH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45854">
        <property type="entry name" value="ASAP FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 14" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 15" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="19">
        <location>
            <begin position="328"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="20">
        <location>
            <begin position="442"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="603"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="18">
        <location>
            <begin position="1093"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="719"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="782"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="726"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="739"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="797"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="820"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="882"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1026"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1051"/>
            <end position="1070"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_053537783.1"/>
        </source>
    </evidence>
    <sequence length="1155" mass="127979" checksum="1B1DB7038AA6A932" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TLL9</accession>
    <name>A0A9F7TLL9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Mpv17-like protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">mp17l</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053539868.1">
        <property type="nucleotide sequence ID" value="XM_053683893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TLL9"/>
    <dbReference type="GeneID" id="100528987"/>
    <dbReference type="CTD" id="100528987"/>
    <dbReference type="OMA" id="CSFTWAN"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061668">
        <property type="term" value="P:mitochondrial ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF39">
        <property type="entry name" value="MPV17-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053539868.1"/>
        </source>
    </evidence>
    <sequence length="195" mass="22471" checksum="CBE6C8F6F5A93A13" modified="2023-06-28" version="1">MNPIRRYPWLTNVTLYSGLYAGGDLMQQYLSNKKDGKIDWRRTRNVAVVAFCFNGNFNFFWMRFLERRLPGSSVSTVLRKLAMDQTISLPLAISAFYTGLSLLEGKDDILEDWRNKFLNTYTTGLMFWPFAQCLNFALVPLYLRTTFTGCCAFAWATFLCFSRQCGDGTLKAALNWIRQLNSEVKVKIQESSKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TNT2</accession>
    <name>A0A9F7TNT2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Mucin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC108280038</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053544094.1">
        <property type="nucleotide sequence ID" value="XM_053688119.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108280038"/>
    <dbReference type="KEGG" id="ipu:108280038"/>
    <dbReference type="OrthoDB" id="6262482at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd19941">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006207">
        <property type="entry name" value="Cys_knot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058753">
        <property type="entry name" value="TIL_OTOGL_Mucin"/>
    </dbReference>
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        <property type="entry name" value="VWF/SSPO/ZAN-like_Cys-rich_dom"/>
    </dbReference>
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        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47246:SF1">
        <property type="entry name" value="MUCIN-19"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25962">
        <property type="entry name" value="TIL_OTOGL_Mucin"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CT"/>
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        <property type="entry name" value="VWC_out"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="VWD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01225">
        <property type="entry name" value="CTCK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039943408" evidence="7">
        <location>
            <begin position="24"/>
            <end position="2043"/>
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        <location>
            <begin position="27"/>
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        </location>
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            <begin position="471"/>
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            <begin position="1503"/>
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            <begin position="1953"/>
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        <location>
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        </location>
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        <location>
            <begin position="1972"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053544094.1"/>
        </source>
    </evidence>
    <sequence length="2043" mass="223384" checksum="86FC3959C2489430" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3TF79</accession>
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        <submittedName>
            <fullName evidence="2 4">COMM domain-containing protein 7</fullName>
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    <gene>
        <name evidence="2" type="primary">COMD7</name>
        <name evidence="4" type="synonym">comd7</name>
        <name evidence="4" type="synonym">commd7</name>
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    <organism evidence="2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Teleostei</taxon>
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            <taxon>Siluriformes</taxon>
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    <reference evidence="2 4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="component" value="Chromosome 15"/>
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        <property type="term" value="F:NF-kappaB binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:tumor necrosis factor-mediated signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="COMM"/>
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    </dbReference>
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        <property type="entry name" value="COMMD7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16231">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 4-8 FAMILY MEMBER"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR16231:SF2">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07258">
        <property type="entry name" value="COMM_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="COMM_HN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="COMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COMM" evidence="1">
        <location>
            <begin position="133"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADO28965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="NP_001187672.1"/>
        </source>
    </evidence>
    <sequence length="200" mass="22799" checksum="78F7D2378747EDB2" modified="2011-01-11" version="1">MLQLHFTKDAVPDNVSSDFQTLNKFSEQQFLSLTDLLYQFLLEPKETERFLAQLNEFAGENGMGAGPLRGLMKSIVLLPQGAMKKNLTAEQVKEDLLSLGLTEDKASHFSDQWRLHYPVLSRLAVAQTLMVNQLVDMEWKFGVTVGTSELQKAGNIFLQLKLVLRKGNTTENVYMELTLPQFYNFLHEMERAEANMDCFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5U5V9</accession>
    <name>W5U5V9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TBC1 domain family member 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TBC1D13</name>
        <name evidence="13" type="synonym">tbc1d13</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Acts as a GTPase-activating protein for RAB35. Together with RAB35 may be involved in regulation of insulin-induced glucose transporter SLC2A4/GLUT4 translocation to the plasma membrane in adipocytes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with RAB1A and RAB10; in a GTP-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT405655">
        <property type="protein sequence ID" value="AHH37273.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017348300.1">
        <property type="nucleotide sequence ID" value="XM_017492811.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U5V9"/>
    <dbReference type="GeneID" id="108278973"/>
    <dbReference type="KEGG" id="ipu:108278973"/>
    <dbReference type="CTD" id="54662"/>
    <dbReference type="OMA" id="TEFPCEE"/>
    <dbReference type="OrthoDB" id="10263206at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000009">
        <property type="entry name" value="TBC1 domain family member 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000019">
        <property type="entry name" value="TBC1 domain family member 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF27">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="10">
        <location>
            <begin position="35"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="148"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AHH37273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017348300.1"/>
        </source>
    </evidence>
    <sequence length="400" mass="46859" checksum="E18A026E7FE40CA0" modified="2014-04-16" version="1">MSSSYKNRIQEFKTSLSEEKIDLKALRELCFGGIPFEGGIRSLCWKILLNFLPLDQTLWDSFLKKQREIYSQFLKEMIIQPGIAKANLGLSREDVTMEDHPLNPNPESRWNNYFKDNEVLLQIDKDVRRLYPDMAFFQRPTEFPCQLILDSQNEYETLRRRVEQTTLKAQTVNRNRSGVTNVSSPGKALNLYPSNEYEVLPNGCEAHWEVVERILFIYAKLNPGIAYVQGMNEIVGPIYYTFATDPNPQWKEHAEADTFFCFTNLMSENRDNFIKSLDDSQCGITYKMESVFSMLKEKDLELYMKLQEQNIKPQYFTFRWLTLLLSQEFLLPDVIRIWDSLFSDQDRFHFLILVCCAMLTLIRNQLLAGDFTTNMRLLQDYPISDVHTILIKAKELQDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5U6S3</accession>
    <name>W5U6S3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Solute carrier family 2, facilitated glucose transporter member 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Glucose transporter type 8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Glucose transporter type X1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">Slc2a8</name>
        <name evidence="22" type="synonym">slc2a8</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Insulin-regulated facilitative hexose transporter that mediates the transport of glucose and fructose. Facilitates hepatic influx of dietary trehalose, which in turn inhibits glucose and fructose influx triggering a starvation signal and hepatic autophagy through activation of AMPK and ULK1. Also able to mediate the transport of dehydroascorbate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-fructose(out) = D-fructose(in)</text>
            <dbReference type="Rhea" id="RHEA:60372"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>D-glucose(out) = D-glucose(in)</text>
            <dbReference type="Rhea" id="RHEA:60376"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>L-dehydroascorbate(out) = L-dehydroascorbate(in)</text>
            <dbReference type="Rhea" id="RHEA:60380"/>
            <dbReference type="ChEBI" id="CHEBI:58539"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>alpha,alpha-trehalose(in) = alpha,alpha-trehalose(out)</text>
            <dbReference type="Rhea" id="RHEA:17629"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="JT406208">
        <property type="protein sequence ID" value="AHH37500.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017307844.1">
        <property type="nucleotide sequence ID" value="XM_017452355.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U6S3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021522"/>
    <dbReference type="GeneID" id="108255953"/>
    <dbReference type="KEGG" id="ipu:108255953"/>
    <dbReference type="CTD" id="29988"/>
    <dbReference type="GeneTree" id="ENSGT00940000158795"/>
    <dbReference type="OMA" id="YWIDYGT"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033300">
        <property type="term" value="F:dehydroascorbic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904659">
        <property type="term" value="P:D-glucose transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17433">
        <property type="entry name" value="MFS_GLUT8_Class3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000055">
        <property type="entry name" value="Facilitated trehalose transporter Tret1-2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF18">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTER MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20 22" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="39"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="311"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="340"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="393"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="429"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="461"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="19">
        <location>
            <begin position="38"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00059062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00077395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00080242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="AHH37500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017307844.1"/>
        </source>
    </evidence>
    <sequence length="501" mass="54730" checksum="6D7DC0962A02A007" modified="2014-04-16" version="1">MNHEEESRPLLAEEENTEHRAEDRSETETYLNNVKNGKLYMATFAAVLGPLSFGFVLGYSSPAIPDLQKIPDPRLRLTAEEGSWFGSVVTIGAGLGGLLGGWVVEKIGRKLSLMFCALPFIFGFTVIIAAQNHWMLYVGRVLTGVASGVTSLVVPLYISETSHERVRGTMGSCVQLMVVTGIMGAYITGLFLDWRWLAVTSSIPPTLMLVCMCFMPETPRYLLSRGKRSEAEEALRFLRGRDTPVEWECARIEAASRYEEGSLSLADLKDPGVYKPLGVGIMMMLLQQFTGINAIMFYAQTIFELAHFQNGSVASVFVGATQVIFTAVAALVMDRAGRKVLLILSGVAMCVSEAAFGIYFKVSTANHGNATLLSTIMEAQDAVSEATHTDLSWLALTSMGIFITGFALGWGPIPWLVMSEIFPTKARGLGSAACVLTNWTCAFIVTKTFQNLTDLLTSAGTFWMFSCLCALNVIFTVFFVPETKGKTLEEIQAHFKGAHSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5UB90</accession>
    <name>W5UB90_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15 17 18">P2Y purinoceptor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">P2RY3</name>
        <name evidence="17 18 19 20" type="synonym">LOC108264849</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17 18 19" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17 18 19">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT410988">
        <property type="protein sequence ID" value="AHH39266.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017322260.1">
        <property type="nucleotide sequence ID" value="XM_017466771.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017322261.1">
        <property type="nucleotide sequence ID" value="XM_017466772.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017322262.1">
        <property type="nucleotide sequence ID" value="XM_017466773.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047011085.1">
        <property type="nucleotide sequence ID" value="XM_047155129.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UB90"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007892"/>
    <dbReference type="GeneID" id="108264849"/>
    <dbReference type="KEGG" id="ipu:108264849"/>
    <dbReference type="GeneTree" id="ENSGT01030000234621"/>
    <dbReference type="OMA" id="ICGGVWL"/>
    <dbReference type="OrthoDB" id="9881476at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001621">
        <property type="term" value="F:G protein-coupled ADP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045029">
        <property type="term" value="F:G protein-coupled UDP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045030">
        <property type="term" value="F:G protein-coupled UTP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905835">
        <property type="term" value="P:cellular response to pyrimidine ribonucleotide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000017">
        <property type="entry name" value="lysophosphatidic acid receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000371">
        <property type="entry name" value="P2Y3_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231:SF16">
        <property type="entry name" value="P2Y PURINOCEPTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231">
        <property type="entry name" value="PURINOCEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01065">
        <property type="entry name" value="P2Y3PRNOCPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="139"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="39"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AHH39266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017322260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017322261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017322262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_047011085.1"/>
        </source>
    </evidence>
    <sequence length="335" mass="38320" checksum="CCDEDC9B86F7C57C" modified="2014-04-16" version="1">MENASSSSSILPSCTYSEDFKRILLPVVYSVVFVLGLPLNFAVILQLFGKRRSLTRTNIYTLNLAAADFLYVCSLPLLIYNYGSRDYWPFGELMCRWVRFQFYSNLHSSILFLTCISVQRYLGICHPLSPWLRKAGRKLAWAVCASVWVIVALLCAPTFEFAAMGIQRNRTVCYELSLPERSQQYYPYGMALTCLGFLLPFMVIVVLYCRMARALCQPGNASGEATKEKKCKAVRMIAIVVLVFAVSFLPFHVTKTMYLLVRTLPDAPCQLRNLLSVVYKSTRPFASMNSVLDPILFYFTQPKFRRGTRNLLLRISTFRDSKPKERTQDLLNKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>W5UBV1</accession>
    <name>W5UBV1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10 12">Protein Jade-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">phf16</name>
        <name evidence="12" type="synonym">jade3</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the JADE family.</text>
    </comment>
    <dbReference type="EMBL" id="JT405403">
        <property type="protein sequence ID" value="AHH37172.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053539577.1">
        <property type="nucleotide sequence ID" value="XM_053683602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UBV1"/>
    <dbReference type="KEGG" id="ipu:108272064"/>
    <dbReference type="OrthoDB" id="20839at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000123">
        <property type="term" value="C:histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15681">
        <property type="entry name" value="PHD_JADE3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Jade family PHD finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Protein Jade-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Enhancer_polycomb-like_N"/>
    </dbReference>
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        <property type="entry name" value="EPHD"/>
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    <dbReference type="InterPro" id="IPR050701">
        <property type="entry name" value="Histone_Mod_Regulator"/>
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        <property type="entry name" value="JADE3_PHD"/>
    </dbReference>
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        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
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        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
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        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793">
        <property type="entry name" value="PHD FINGER PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793:SF27">
        <property type="entry name" value="PROTEIN JADE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10513">
        <property type="entry name" value="EPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13831">
        <property type="entry name" value="PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="8">
        <location>
            <begin position="201"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="9">
        <location>
            <begin position="253"/>
            <end position="367"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="369"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="541"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="551"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="766"/>
            <end position="777"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038371"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AHH37172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053539577.1"/>
        </source>
    </evidence>
    <sequence length="777" mass="87007" checksum="71CDFDC67F40AE6B" modified="2014-04-16" version="1"> </sequence>
</entry>
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    <accession>W5UGM4</accession>
    <name>W5UGM4_ICTPU</name>
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            <fullName evidence="2 4">Calcium signal-modulating cyclophilin ligand</fullName>
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    <gene>
        <name evidence="2" type="primary">CAMLG</name>
        <name evidence="4" type="synonym">camlg</name>
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    <organism evidence="2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT414692">
        <property type="protein sequence ID" value="AHH40724.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00390000015996"/>
    <dbReference type="OMA" id="SFMKPPE"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071816">
        <property type="term" value="P:tail-anchored membrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016719">
        <property type="entry name" value="CAMLG"/>
    </dbReference>
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        <property type="entry name" value="CALCIUM-SIGNAL MODULATING CYCLOPHILIN LIGAND CAML"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15026:SF0">
        <property type="entry name" value="GUIDED ENTRY OF TAIL-ANCHORED PROTEINS FACTOR CAMLG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Get2_like"/>
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    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
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        <location>
            <begin position="79"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AHH40724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017349120.1"/>
        </source>
    </evidence>
    <sequence length="272" mass="31152" checksum="371B19C87308B1A8" modified="2014-04-16" version="1">MEAEISTPEERGVSAAQRRAEIRRRKLLMNSEQRMNRIVGFNKNDGENSEVQRVTEPRFHLDLDRNECFSSSVSTKRVSPFVSEARSEGSMLDERREEEGEDTERGVRLRPRGETSSEERSRSPRRGINTYLSRFDDAIKLRGQLTNEKASQESSTDVDEFDSFRLFRLLGSVVLAVCVRIFVCHYLSIFAPFLTLELAYMGLYKYFPKVDRKMKTTVLSAALLLSGIPAEVISRSAETYRTMGDVFSDLCVYFFTFITCHEIITAFSSHST</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>W5UI02</accession>
    <name>W5UI02_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox protein aristaless-like 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">Alx4</name>
        <name evidence="20" type="synonym">alx4a</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="11">Binds DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="JT415515">
        <property type="protein sequence ID" value="AHH41722.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017334406.1">
        <property type="nucleotide sequence ID" value="XM_017478917.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UI02"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006604"/>
    <dbReference type="GeneID" id="108271351"/>
    <dbReference type="KEGG" id="ipu:108271351"/>
    <dbReference type="CTD" id="100006399"/>
    <dbReference type="OMA" id="PCYGKDN"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000127">
        <property type="entry name" value="homeobox protein aristaless-like 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003654">
        <property type="entry name" value="OAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050649">
        <property type="entry name" value="Paired_Homeobox_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329">
        <property type="entry name" value="HOMEOBOX PROTEIN ARISTALESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329:SF322">
        <property type="entry name" value="HOMEOBOX PROTEIN ARISTALESS-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03826">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50803">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 13 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 13 14" id="KW-0371">Homeobox</keyword>
    <keyword evidence="10 13 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="16">
        <location>
            <begin position="181"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="OAR" evidence="17">
        <location>
            <begin position="356"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="13">
        <location>
            <begin position="183"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="30"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="129"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="47"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AHH41722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017334406.1"/>
        </source>
    </evidence>
    <sequence length="376" mass="41070" checksum="37156E7A5EA3E407" modified="2014-04-16" version="1">MNAETCVSYCDMTSMDSYYSASAAQARDQQANNPFRTFPTSSDTKYSPTFLSSSTNSTSSSKAPAYGEKSRSPFQQECQPLDGTAGEGTFNKYHLFMHRSSCKTPPESSKLHQDSNGGLISCYGKDTTGLTDSDLPANTDPTGMDGSYLSVKDPGAKVPQDRPSSELASPLDKGEGESNKGKKRRNRTTFTSYQLEELEKVFQKTHYPDVYAREQLALRTDLTEARVQVWFQNRRAKWRKRERFGQMQQVRTHFSTAYELPLLTRPENYAQIQNPSWIGGSSAASPVPGCVVPCDSVTSCMTPHPHAASGVSDFLGVPSPGGHMGQSHMGSLFGSPGMSGGINGYDLNMDPDRKSSSIAALRMKAKEHSAAISWAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5UJE6</accession>
    <name>W5UJE6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5 7 8 9">Ras-related protein Rab-5B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RAB5B</name>
        <name evidence="7 8 9" type="synonym">rab5b</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7 8 9" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7 8 9">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="JT412247">
        <property type="protein sequence ID" value="AHH39767.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017325093.1">
        <property type="nucleotide sequence ID" value="XM_017469604.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047012092.1">
        <property type="nucleotide sequence ID" value="XM_047156136.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047012093.1">
        <property type="nucleotide sequence ID" value="XM_047156137.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UJE6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035430"/>
    <dbReference type="GeneID" id="108266395"/>
    <dbReference type="KEGG" id="ipu:108266395"/>
    <dbReference type="CTD" id="5869"/>
    <dbReference type="GeneTree" id="ENSGT00940000166096"/>
    <dbReference type="OMA" id="IFNIMAQ"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01860">
        <property type="entry name" value="Rab5_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000995">
        <property type="entry name" value="RAB5B, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="184"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AHH39767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017325093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_047012092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_047012093.1"/>
        </source>
    </evidence>
    <sequence length="214" mass="23739" checksum="ED0E052CF9F110B1" modified="2014-04-16" version="1">MSNRGNARTNGNLPQTKICQFKLVLLGDMAVGKSSLVLRFVKGQFDEFQETTIGAAFLAQSVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVFDITKPETFERAKAWVKELQRQASPNIVIALAGNKSDLADKRLVEYEEAQTYAEDTGLLFMETSAKTAMNVNELFLAIAKKMPKTDTQNPTHAARHRGVNLHDPDAQSSRSCCGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5UJT2</accession>
    <name>W5UJT2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5 7">SRA stem-loop-interacting RNA-binding protein, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">SLIRP</name>
        <name evidence="7" type="synonym">slirp</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="5">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
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    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
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    </reference>
    <dbReference type="EMBL" id="JT417470">
        <property type="protein sequence ID" value="AHH42389.1"/>
        <property type="molecule type" value="mRNA"/>
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        <property type="nucleotide sequence ID" value="XM_017476626.3"/>
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    <dbReference type="AlphaFoldDB" id="W5UJT2"/>
    <dbReference type="GeneID" id="108270198"/>
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    <dbReference type="GeneTree" id="ENSGT00390000008624"/>
    <dbReference type="OMA" id="GFVMFSQ"/>
    <dbReference type="OrthoDB" id="6159137at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12242">
        <property type="entry name" value="RRM_SLIRP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SRA stem-loop interacting RNA binding protein"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052462">
        <property type="entry name" value="SLIRP/GR-RBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034152">
        <property type="entry name" value="SLIRP_RRM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48027">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN 87F-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="RRM_1"/>
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    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
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        <property type="entry name" value="RRM"/>
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    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
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        <location>
            <begin position="9"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AHH42389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017332115.1"/>
        </source>
    </evidence>
    <sequence length="100" mass="11517" checksum="FA2019E2EA0850C1" modified="2014-04-16" version="1">MAASSKKVFELFVSKVPWTVATKEIKEYFGQFGQVKKCLLVFDKDTGFHRGFCWIGYSSEEGLQNALQKDPHIMEGSRLQVQKNRRPFMGRKPNKEADES</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5UJZ1</accession>
    <name>W5UJZ1_ICTPU</name>
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        <submittedName>
            <fullName evidence="6 8">Gem-associated protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Gemin5</name>
        <name evidence="8" type="synonym">gemin5</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT417375">
        <property type="protein sequence ID" value="AHH42362.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017347693.1">
        <property type="nucleotide sequence ID" value="XM_017492204.3"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0032797">
        <property type="term" value="C:SMN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056432">
        <property type="entry name" value="Beta-prop_GEMI5_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056424">
        <property type="entry name" value="Beta-prop_GEMI5_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056420">
        <property type="entry name" value="GEMI5_RBS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052640">
        <property type="entry name" value="Gemin-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056421">
        <property type="entry name" value="TPR_GEMI5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46362">
        <property type="entry name" value="GEM-ASSOCIATED PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46362:SF1">
        <property type="entry name" value="GEM-ASSOCIATED PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23770">
        <property type="entry name" value="Beta-prop_RIG_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23775">
        <property type="entry name" value="Beta-prop_RIG_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23777">
        <property type="entry name" value="GEMI5_RBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23774">
        <property type="entry name" value="TPR_GEMI5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="50"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="222"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="475"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="616"/>
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        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="659"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="696"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1283"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1347"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="715"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="744"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="799"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1362"/>
            <end position="1372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AHH42362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017347693.1"/>
        </source>
    </evidence>
    <sequence length="1459" mass="161725" checksum="B99C2CC732235B7B" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5ULC4</accession>
    <name>W5ULC4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">NADH-cytochrome b5 reductase</fullName>
            <ecNumber evidence="28">1.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="primary">Cyb5r3</name>
        <name evidence="32" type="synonym">LOC108279735</name>
    </gene>
    <organism evidence="30">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
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            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="30">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="31" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="31">SDA103</strain>
        </source>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="32">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="22">Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>2 Fe(III)-[cytochrome b5] + NADH = 2 Fe(II)-[cytochrome b5] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46680"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.6.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:46681"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 27 28">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="25">Component of a complex composed of cytochrome b5, NADH-cytochrome b5 reductase (CYB5R3) and MTARC2. Interacts with MTLN; the interaction is required to maintain cellular lipid composition and leads to stimulation of mitochondrial respiratory complex I activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="23">Endoplasmic reticulum membrane</location>
            <topology evidence="23">Lipid-anchor</topology>
            <orientation evidence="23">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Mitochondrion outer membrane</location>
            <topology evidence="24">Lipid-anchor</topology>
            <orientation evidence="24">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 28">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.2" evidence="28"/>
    <dbReference type="EMBL" id="JT418796">
        <property type="protein sequence ID" value="AHH42847.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017349654.1">
        <property type="nucleotide sequence ID" value="XM_017494165.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ULC4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000025967"/>
    <dbReference type="GeneID" id="108279735"/>
    <dbReference type="KEGG" id="ipu:108279735"/>
    <dbReference type="OMA" id="VQIFMCG"/>
    <dbReference type="OrthoDB" id="432685at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090524">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NADH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006695">
        <property type="term" value="P:cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000021">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000005">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001834">
        <property type="entry name" value="CBR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF121">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0152">Cholesterol biosynthesis</keyword>
    <keyword evidence="4" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 27 28" id="KW-0274">FAD</keyword>
    <keyword evidence="6 27 28" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="21" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="15 28" id="KW-0520">NAD</keyword>
    <keyword evidence="13 28" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="20" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="14" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="19" id="KW-1207">Sterol metabolism</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="29">
        <location>
            <begin position="38"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00037417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00037811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00037821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00047026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00047465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR601834-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="RuleBase" id="RU361226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="AHH42847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="RefSeq" id="XP_017349654.1"/>
        </source>
    </evidence>
    <sequence length="299" mass="33781" checksum="8BEB3DB3ACBA72CF" modified="2014-04-16" version="1">MLSYIIGLIRGGFDSIIGLIMRLLGAKRKPAITLEDPNEKYPLRLIDKEIISHDTRKFRFALKSPDYVLGLPIGQHIYLSARIDGGLVVRPYTPVSSDDDKGFVDLVVKIYYKNTHPKFPEGGKMSQYLDSLRIGDMIDFRGPSGLLVYKGQGVFEIKPEKKAPAEVKTAKHLGMIAGGTGITPMLQIIRAIMKDPKDKTICYLLFANQSEKDILLRTDLEEIQANHPDRFKLWYTLDKVPDDWEYSQGFISEQMVQNRLPPPGDDTLILMCGPPPMIQFACNPNLDKVGHSLSRRFVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>W5UM58</accession>
    <name>W5UM58_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12 14">Paired mesoderm homeobox protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PRRX1</name>
        <name evidence="14" type="synonym">prrx1b</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="JT414717">
        <property type="protein sequence ID" value="AHH40732.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017310892.1">
        <property type="nucleotide sequence ID" value="XM_017455403.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UM58"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012354"/>
    <dbReference type="GeneID" id="108257538"/>
    <dbReference type="CTD" id="337089"/>
    <dbReference type="GeneTree" id="ENSGT00940000159466"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000066">
        <property type="entry name" value="Paired mesoderm homeobox protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003654">
        <property type="entry name" value="OAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043378">
        <property type="entry name" value="PRRX1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46385:SF1">
        <property type="entry name" value="PAIRED MESODERM HOMEOBOX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46385">
        <property type="entry name" value="PAIRED MESODERM HOMEOBOX PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03826">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50803">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="95"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="OAR" evidence="11">
        <location>
            <begin position="225"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="97"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="53"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AHH40732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017310892.1"/>
        </source>
    </evidence>
    <sequence length="246" mass="27490" checksum="8495C9D33D911B8A" modified="2014-04-16" version="1">MTSSYAHVMDRRAVAVPSRLESPITANVDNLQAKKNFSVSHLLDLEEAGEMVGAQAEESVAESGRTMLESPGLTSGSDTAQPENEQLNAEEKKKRKQRRNRTTFNSSQLQALERVFERTHYPDAFVREDLARRVNLTEARVQVWFQNRRAKFRRNERAMLASKNASLLKSYSGDVTAVEQPIVPRPAPRPNDYLSWGSATPYSTVAAYPPTCVNTTSTQGMNMANSIANLRLKAKEYSLNQVPTVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5USI7</accession>
    <name>W5USI7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">polynucleotide adenylyltransferase</fullName>
            <ecNumber evidence="5">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">PAPOLG</name>
        <name evidence="23 24 25 26 27 28 29 30" type="synonym">papolg</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23 24 25" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23 24 25">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the poly(A) polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="5"/>
    <dbReference type="EMBL" id="JT418040">
        <property type="protein sequence ID" value="AHH42593.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017319998.1">
        <property type="nucleotide sequence ID" value="XM_017464509.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017319999.1">
        <property type="nucleotide sequence ID" value="XM_017464510.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017320000.1">
        <property type="nucleotide sequence ID" value="XM_017464511.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053535724.1">
        <property type="nucleotide sequence ID" value="XM_053679749.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053535725.1">
        <property type="nucleotide sequence ID" value="XM_053679750.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053535726.1">
        <property type="nucleotide sequence ID" value="XM_053679751.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053535727.1">
        <property type="nucleotide sequence ID" value="XM_053679752.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053535728.1">
        <property type="nucleotide sequence ID" value="XM_053679753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5USI7"/>
    <dbReference type="GeneID" id="108263564"/>
    <dbReference type="KEGG" id="ipu:108263564"/>
    <dbReference type="CTD" id="64895"/>
    <dbReference type="GeneTree" id="ENSGT00940000156467"/>
    <dbReference type="OrthoDB" id="412748at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000002">
        <property type="entry name" value="Poly(A) polymerase alpha, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000001">
        <property type="entry name" value="Putative poly(A) polymerase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.590:FF:000001">
        <property type="entry name" value="Putative poly(A) polymerase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.590">
        <property type="entry name" value="Poly(A) polymerase predicted RNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011068">
        <property type="entry name" value="NuclTrfase_I-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007012">
        <property type="entry name" value="PolA_pol_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048840">
        <property type="entry name" value="PolA_pol_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007010">
        <property type="entry name" value="PolA_pol_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682">
        <property type="entry name" value="POLY A POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682:SF6">
        <property type="entry name" value="POLY(A) POLYMERASE GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04928">
        <property type="entry name" value="PAP_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20750">
        <property type="entry name" value="PAP_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04926">
        <property type="entry name" value="PAP_RNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55003">
        <property type="entry name" value="PAP/Archaeal CCA-adding enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
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            <end position="233"/>
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            <end position="382"/>
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            <end position="451"/>
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            <dbReference type="EMBL" id="AHH42593.1"/>
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        <source>
            <dbReference type="RefSeq" id="XP_053535725.1"/>
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    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_053535726.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
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    <evidence type="ECO:0000313" key="30">
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            <dbReference type="RefSeq" id="XP_053535728.1"/>
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        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
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                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
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    <reference evidence="9" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
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                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
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                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
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        <source>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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    <reference evidence="34" key="1">
        <citation type="journal article" date="2000" name="Vet. Immunol. Immunopathol." volume="76" first="61" last="74">
            <title>Molecular characterization and leukocyte distribution of a teleost beta1 integrin molecule.</title>
            <authorList>
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                <person name="Noya M."/>
                <person name="Ainsworth A.J."/>
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            <dbReference type="PubMed" id="10973686"/>
            <dbReference type="DOI" id="10.1016/S0165-2427(00)00200-2"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="33">SDA103</strain>
        </source>
    </reference>
    <reference evidence="34" key="4">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="26">Cell membrane</location>
            <topology evidence="26">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Invadopodium membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Ruffle membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Melanosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 26">Belongs to the integrin beta chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="NP_001187015.2">
        <property type="nucleotide sequence ID" value="NM_001200086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PET0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018542"/>
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    <dbReference type="KEGG" id="ipu:100304492"/>
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    <dbReference type="OrthoDB" id="410592at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
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    <dbReference type="GO" id="GO:0019960">
        <property type="term" value="F:C-X3-C chemokine binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098639">
        <property type="term" value="F:collagen binding involved in cell-matrix adhesion"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001968">
        <property type="term" value="F:fibronectin binding"/>
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    <dbReference type="GO" id="GO:0005178">
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    <dbReference type="GO" id="GO:0043236">
        <property type="term" value="F:laminin binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0033627">
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    </dbReference>
    <dbReference type="GO" id="GO:0016477">
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    </dbReference>
    <dbReference type="GO" id="GO:0098609">
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    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="4"/>
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        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="EGF_integrin_2"/>
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        <property type="entry name" value="Integrin_beta_N"/>
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        <property type="entry name" value="Integrin_bsu"/>
    </dbReference>
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        <property type="entry name" value="Integrin_bsu_cyt_dom"/>
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        <property type="entry name" value="Integrin_bsu_tail"/>
    </dbReference>
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        <property type="entry name" value="Integrin_bsu_tail_dom_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Integrin_dom_sf"/>
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        <property type="entry name" value="Integrin_I-EGF_CS"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="INB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0106">Calcium</keyword>
    <keyword evidence="16 26" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="17" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="24" id="KW-0966">Cell projection</keyword>
    <keyword evidence="21 25" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="23" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
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    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0517">Myogenesis</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
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    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 28 34" id="KW-0732">Signal</keyword>
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    <feature type="signal peptide" evidence="28 34">
        <location>
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        </location>
    </feature>
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    </feature>
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    </feature>
    <feature type="domain" description="PSI" evidence="30">
        <location>
            <begin position="26"/>
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    </feature>
    <feature type="domain" description="Integrin beta subunit VWA" evidence="29">
        <location>
            <begin position="34"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin beta subunit tail" evidence="32">
        <location>
            <begin position="640"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin beta subunit cytoplasmic" evidence="31">
        <location>
            <begin position="751"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="27"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="38"/>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="486"/>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="538"/>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="555"/>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="592"/>
            <end position="599"/>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    <feature type="disulfide bond" evidence="25">
        <location>
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    <feature type="disulfide bond" evidence="25">
        <location>
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    <feature type="disulfide bond" evidence="25">
        <location>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    <evidence type="ECO:0000256" key="6">
        <source>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR002512-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU000633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="SMART" id="SM00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="SMART" id="SM00423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="SMART" id="SM01241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="SMART" id="SM01242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="RefSeq" id="NP_001187015.2"/>
        </source>
    </evidence>
    <sequence length="797" mass="88121" checksum="C0F4A889644D8C80" modified="2017-12-20" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PIE2</accession>
    <name>A0A2D0PIE2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="14">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">smurf2</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6 14">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane raft</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="14"/>
    <dbReference type="RefSeq" id="XP_017305629.1">
        <property type="nucleotide sequence ID" value="XM_017450140.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PIE2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000011651"/>
    <dbReference type="GeneID" id="108254810"/>
    <dbReference type="KEGG" id="ipu:108254810"/>
    <dbReference type="CTD" id="64750"/>
    <dbReference type="OMA" id="LIFLICE"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046332">
        <property type="term" value="F:SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd08382">
        <property type="entry name" value="C2_Smurf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000017">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000026">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000047">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000024">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000014">
        <property type="entry name" value="E3 ubiquitin-protein ligase SMURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000007">
        <property type="entry name" value="E3 ubiquitin-protein ligase SMURF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024928">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF300">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SMURF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001569">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 14 16" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="C2" evidence="17">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="18">
        <location>
            <begin position="157"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="18">
        <location>
            <begin position="251"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="18">
        <location>
            <begin position="297"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="19">
        <location>
            <begin position="430"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="15 16">
        <location>
            <position position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR001569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001569-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017305629.1"/>
        </source>
    </evidence>
    <sequence length="764" mass="87315" checksum="200B037850F39811" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PIX4</accession>
    <name>A0A2D0PIX4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Metalloproteinase inhibitor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC108254913</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protease inhibitor I35 (TIMP) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017305854.1">
        <property type="nucleotide sequence ID" value="XM_017450365.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PIX4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000017287"/>
    <dbReference type="GeneID" id="108254913"/>
    <dbReference type="KEGG" id="ipu:108254913"/>
    <dbReference type="OMA" id="CECKIST"/>
    <dbReference type="OrthoDB" id="6041373at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008191">
        <property type="term" value="F:metalloendopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051045">
        <property type="term" value="P:negative regulation of membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.370.10">
        <property type="entry name" value="Tissue inhibitor of metalloproteinase-1. Chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001134">
        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001820">
        <property type="entry name" value="TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027465">
        <property type="entry name" value="TIMP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11844">
        <property type="entry name" value="METALLOPROTEASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11844:SF25">
        <property type="entry name" value="NTR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00965">
        <property type="entry name" value="TIMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00206">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50189">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0481">Metalloenzyme inhibitor</keyword>
    <keyword evidence="4 13" id="KW-0483">Metalloprotease inhibitor</keyword>
    <keyword evidence="5 13" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013402031" evidence="10">
        <location>
            <begin position="26"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="NTR" evidence="11">
        <location>
            <begin position="26"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared with metalloproteinase partner</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="26"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="28"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="38"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="151"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="156"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601820-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601820-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017305854.1"/>
        </source>
    </evidence>
    <sequence length="229" mass="26322" checksum="6F0A47B3D7151A11" modified="2017-12-20" version="1">MDQVKVQLFLLIVLALGITNPMAEGCSCIHAHFQELFCHSEIVIRAKIIGEKIISPSLHGEKVIEYKIKLIKMFKGFDKVDTIQYIYTSIHESLCGIKLDAKNRGQYLLSGYLWHDGKVLVELCGIEERWENLSLAQKKNLKYRYQKGCECKISTCSKRHCRANANECAWLPYNSQHLQSVCIKRTNGTCNWYIDKSDSHTQLNVRQNGMRETSGILEVREPKPTETLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PKH0</accession>
    <name>A0A2D0PKH0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BOLA class I histocompatibility antigen, alpha chain BL3-7 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC108255571</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the MHC class I family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017307123.1">
        <property type="nucleotide sequence ID" value="XM_017451634.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PKH0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034059"/>
    <dbReference type="GeneID" id="108255571"/>
    <dbReference type="KEGG" id="ipu:108255571"/>
    <dbReference type="OrthoDB" id="8936120at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07698">
        <property type="entry name" value="IgC1_MHC_I_alpha3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.500.10:FF:000001">
        <property type="entry name" value="H-2 class I histocompatibility antigen, alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001039">
        <property type="entry name" value="MHC_I_a_a1/a2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF237">
        <property type="entry name" value="MHC CLASS I ANTIGEN TRANSCRIPT VARIANT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00129">
        <property type="entry name" value="MHC_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01638">
        <property type="entry name" value="MHCCLASSI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012067596" evidence="5">
        <location>
            <begin position="24"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="202"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="346"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="346"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="357"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="374"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017307123.1"/>
        </source>
    </evidence>
    <sequence length="395" mass="44458" checksum="4B628437ACBFBE2A" modified="2017-12-20" version="1">MKDNSAMLKLLLFLTFPAHLSSADTHTLQYLYTAVTPGVNFTAVGLVDGEQFVFYDINMTRRISEWMKKAEDDDPLYRQREMEHVKNQQDSFQYIVARVMKIFNQPKGIHIFQRIYGCERDDDGTTRGYDEYGYDGEDFISLDLKTGTWTAANDKAEIFINEWDPTGEKAKHWKDFLEKDCIDQLQKVLNYGKETLERKVPPTASVFQKHSSPEVVCHATGFFPKTVMITWQKDGEDVHEDVDLNDTLPNQDGSFQKRSILTVSAEDLQKHTYTCVIQHSSLEKEIVLREEDIRILNPDRRTGGGSGGGSGGAPIGIIVGVVAALVVVVFVGIVVWKKKNSGEEKKTLSSREKVKLQTQSRRSLHTRSSLRQLATSEMMGSSTDGSASGEAESEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PRB6</accession>
    <name>A0A2D0PRB6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Raftlin isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC108256588</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the raftlin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017309113.1">
        <property type="nucleotide sequence ID" value="XM_017453624.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PRB6"/>
    <dbReference type="GeneID" id="108256588"/>
    <dbReference type="OrthoDB" id="9942562at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028169">
        <property type="entry name" value="Raftlin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17601:SF3">
        <property type="entry name" value="RAFTLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17601">
        <property type="entry name" value="RAFTLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15250">
        <property type="entry name" value="Raftlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="184"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="457"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="194"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="220"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="231"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="270"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="466"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="532"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="543"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="573"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017309113.1"/>
        </source>
    </evidence>
    <sequence length="623" mass="68409" checksum="567C26F72740413E" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PS84</accession>
    <name>A0A2D0PS84_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA polymerase kappa</fullName>
            <ecNumber evidence="4">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">polk</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">SDA103</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20 26">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA polymerase type-Y family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017307945.1">
        <property type="nucleotide sequence ID" value="XM_017452456.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PS84"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019091"/>
    <dbReference type="GeneID" id="108256008"/>
    <dbReference type="KEGG" id="ipu:108256008"/>
    <dbReference type="CTD" id="51426"/>
    <dbReference type="OMA" id="ANESTHM"/>
    <dbReference type="OrthoDB" id="1747274at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03586">
        <property type="entry name" value="PolY_Pol_IV_kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.810:FF:000001">
        <property type="entry name" value="DNA polymerase kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.100:FF:000005">
        <property type="entry name" value="DNA polymerase kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.810:FF:000003">
        <property type="entry name" value="DNA polymerase kappa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000039">
        <property type="entry name" value="Polymerase (DNA directed) kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000151">
        <property type="entry name" value="Polymerase (DNA directed) kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.60:FF:000002">
        <property type="entry name" value="Polymerase (DNA directed) kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.100">
        <property type="entry name" value="DNA polymerase, Y-family, little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01113">
        <property type="entry name" value="DNApol_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036775">
        <property type="entry name" value="DNA_pol_Y-fam_lit_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017961">
        <property type="entry name" value="DNA_pol_Y-fam_little_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050116">
        <property type="entry name" value="DNA_polymerase-Y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022880">
        <property type="entry name" value="DNApol_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006642">
        <property type="entry name" value="Rad18_UBZ4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11076:SF33">
        <property type="entry name" value="DNA POLYMERASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11076">
        <property type="entry name" value="DNA REPAIR POLYMERASE UMUC / TRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11799">
        <property type="entry name" value="IMS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036603">
        <property type="entry name" value="DPol_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00734">
        <property type="entry name" value="ZnF_Rad18"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100879">
        <property type="entry name" value="Lesion bypass DNA polymerase (Y-family), little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="21" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0227">DNA damage</keyword>
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</entry>
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                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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    </evidence>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PXZ6</accession>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017310914.2">
        <property type="nucleotide sequence ID" value="XM_017455425.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PXZ6"/>
    <dbReference type="GeneID" id="108257552"/>
    <dbReference type="KEGG" id="ipu:108257552"/>
    <dbReference type="OrthoDB" id="9049620at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16040">
        <property type="entry name" value="SPRY_PRY_SNTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF5">
        <property type="entry name" value="E3 UBIQUITIN_ISG15 LIGASE TRIM25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="53"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="9">
        <location>
            <begin position="187"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="10">
        <location>
            <begin position="415"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="235"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="300"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017310914.2"/>
        </source>
    </evidence>
    <sequence length="613" mass="70586" checksum="D7E467CE38C6FB32" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PY64</accession>
    <name>A0A2D0PY64_ICTPU</name>
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        <recommendedName>
            <fullName evidence="4">Integrator complex subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ints7</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Integrator subunit 7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017311438.1">
        <property type="nucleotide sequence ID" value="XM_017455949.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PY64"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032538"/>
    <dbReference type="GeneID" id="108257853"/>
    <dbReference type="KEGG" id="ipu:108257853"/>
    <dbReference type="CTD" id="25896"/>
    <dbReference type="OMA" id="GITWCTG"/>
    <dbReference type="OrthoDB" id="1921953at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034472">
        <property type="term" value="P:snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033060">
        <property type="entry name" value="INTS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054519">
        <property type="entry name" value="INTS7_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056517">
        <property type="entry name" value="INTS7_HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056516">
        <property type="entry name" value="INTS7_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13322">
        <property type="entry name" value="C1ORF73 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13322:SF2">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22965">
        <property type="entry name" value="INTS7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24437">
        <property type="entry name" value="INTS7_HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24436">
        <property type="entry name" value="INTS7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrator complex subunit 7 N-terminal" evidence="9">
        <location>
            <begin position="26"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Integrator complex subunit 7 helical bundle" evidence="10">
        <location>
            <begin position="539"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="domain" description="Integrator complex subunit 7 C-terminal" evidence="8">
        <location>
            <begin position="805"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="931"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="937"/>
            <end position="950"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017311438.1"/>
        </source>
    </evidence>
    <sequence length="958" mass="105475" checksum="A5DE50AA3C72D92D" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q2W4</accession>
    <name>A0A2D0Q2W4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Antigen peptide transporter 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC108258487</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <dbReference type="STRING" id="7998.ENSIPUP00000032111"/>
    <dbReference type="GeneID" id="108258487"/>
    <dbReference type="KEGG" id="ipu:108258487"/>
    <dbReference type="OMA" id="ERQRMTI"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="ABC_6TM_TAP2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ABC transporter B family member 25"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ATP-binding cassette sub-family B member 8, mitochondrial"/>
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        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="AAA+_ATPase"/>
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        <property type="entry name" value="ABC1_TM_dom"/>
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        <property type="entry name" value="ABC1_TM_sf"/>
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        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
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    </dbReference>
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        <property type="entry name" value="Type_1_exporter"/>
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        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
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    </dbReference>
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        <property type="entry name" value="TRANSPORTER 2, ATP BINDING CASSETTE SUBFAMILY B"/>
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        <property type="entry name" value="ABC_membrane"/>
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    </dbReference>
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        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002773">
        <property type="entry name" value="ABC_prm/ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ABC_TRANSPORTER_2"/>
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    </dbReference>
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    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
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            <end position="437"/>
        </location>
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            <end position="705"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017312649.1"/>
        </source>
    </evidence>
    <sequence length="715" mass="80481" checksum="A4B5FAB55841F958" modified="2017-12-20" version="1"> </sequence>
</entry>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4 5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017313208.1">
        <property type="nucleotide sequence ID" value="XM_017457719.3"/>
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    <dbReference type="RefSeq" id="XP_017313209.1">
        <property type="nucleotide sequence ID" value="XM_017457720.3"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Fibrinogen-like_C"/>
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        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR050373">
        <property type="entry name" value="Fibrinogen_C-term_domain"/>
    </dbReference>
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        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143:SF225">
        <property type="entry name" value="MICROFIBRIL-ASSOCIATED GLYCOPROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fibrinogen_C"/>
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        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
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        </location>
    </feature>
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            <begin position="20"/>
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        </location>
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            <begin position="22"/>
            <end position="257"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017313208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017313209.1"/>
        </source>
    </evidence>
    <sequence length="264" mass="30006" checksum="EE702C40B10D5034" modified="2017-12-20" version="1">MNHIMLLLLVVALSSLVHSAVLVNTSLPIDCEDVYNLKKSSAKNATVPSGVYTIYPAGPNKTVKVYCDMGCLETGGHDDGKWTVIQRRIDGNLSFYRPWEHYKNGFGDASGEYWLGLENIFLMTYREKYQLRVEMEDFERGSAYAQYTQFYIDPESSNYKLNIAGYVNGGAGDSLSYVNGRDFATFDKDPTGNCAETNSGGFWYYGYWYYNACHLANPNGLYKYGNVGTAYTGIMWQSWKGYYYSLKTIVMKIKRMSLEDLENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q3K2</accession>
    <name>A0A2D0Q3K2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein LOC108258246</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC108258246</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SDA103</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017312266.1">
        <property type="nucleotide sequence ID" value="XM_017456777.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q3K2"/>
    <dbReference type="GeneID" id="108258246"/>
    <dbReference type="KEGG" id="ipu:108258246"/>
    <dbReference type="OrthoDB" id="8446971at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34488">
        <property type="entry name" value="SI:CH211-245H14.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34488:SF1">
        <property type="entry name" value="SI:CH211-245H14.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_017312266.1"/>
        </source>
    </evidence>
    <sequence length="212" mass="24476" checksum="DD0BC3AFE7D3231F" modified="2017-12-20" version="1">MDFISARIARYRQQVVQCTPVFLYKHTFTTKVYVENRGNIKNVCDEIIERLSELPNSISVSNEEECDVKVVFCPIVSRAGTDIEAVLSRLTDGKKTIVVVLHHTFSTDYIPPSSSRYERKDLKMVDMLFHEDSGLLNCSKNNEAISTAGNWLKICSKYQRGVCRKRVWAVAAVAVIGSRFLYRIWTGHWLLQSEIRFINFVIRMRTKTNVYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q3Q6</accession>
    <name>A0A2D0Q3Q6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methionine aminopeptidase</fullName>
            <ecNumber evidence="7">3.4.11.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">metap1d</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="6">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="7"/>
    <dbReference type="RefSeq" id="XP_017312922.1">
        <property type="nucleotide sequence ID" value="XM_017457433.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q3Q6"/>
    <dbReference type="GeneID" id="108258653"/>
    <dbReference type="KEGG" id="ipu:108258653"/>
    <dbReference type="CTD" id="254042"/>
    <dbReference type="OrthoDB" id="3209743at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004239">
        <property type="term" value="F:initiator methionyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01086">
        <property type="entry name" value="MetAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000011">
        <property type="entry name" value="Methionine aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01974">
        <property type="entry name" value="MetAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002467">
        <property type="entry name" value="Pept_M24A_MAP1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00500">
        <property type="entry name" value="met_pdase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43330">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43330:SF8">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 1D, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00680">
        <property type="entry name" value="MAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6 7" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6 7" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Peptidase M24" evidence="8">
        <location>
            <begin position="97"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017312922.1"/>
        </source>
    </evidence>
    <sequence length="346" mass="38570" checksum="7AE3EFA1829169B1" modified="2017-12-20" version="1">MAAPCVTVLRRALFGSGLSQRAVRLPGCQRCPRSGHCQQRRHFFWRKGTSSHSVVRPAVVRPPYPVPKHIQKPGYVSSSSVPEWPDYIEINDEEQIEGLRRACQLARHILLLAGSSLKVGMTTDEIDFIVHQETIRHNAYPSPLHYGGFPKSVCTSVNNVVCHGIPDSRPLQDGDIINIDVTVYLEGYHGDTSETFLIGSVDEQGRKLVDVARQCRDEAIAACGPERPLRVIGNTISEIALSNGFCVCPYFIGHGIGSYFHGHPEIWHHANDNDLLMEEGMAFTIEPILMEGTSEFRILSDKWTAVSIDDKRFSSALHTFRSAQFEHTVIITSDGVEILTKLPDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q508</accession>
    <name>A0A2D0Q508_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">5'-AMP-activated protein kinase subunit gamma-1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">prkag3a</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase gamma subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017312440.1">
        <property type="nucleotide sequence ID" value="XM_017456951.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q508"/>
    <dbReference type="GeneID" id="108258362"/>
    <dbReference type="KEGG" id="ipu:108258362"/>
    <dbReference type="CTD" id="553319"/>
    <dbReference type="OrthoDB" id="449052at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04618">
        <property type="entry name" value="CBS_euAMPK_gamma-like_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04641">
        <property type="entry name" value="CBS_euAMPK_gamma-like_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF41">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE SUBUNIT GAMMA-1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="144"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="219"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="291"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017312440.1"/>
        </source>
    </evidence>
    <sequence length="354" mass="40466" checksum="99726C2A2AB700C8" modified="2017-12-20" version="1">MRKADTRTQARARFHTMESVTQEVCPDPHDINNSATDPGAEVYLDFMKKHCCYDAIPTSCKLIIFDTALQVKKAFFALVANSLRAAPLWDSKLQRFVGMLTITDFINILHRYYKSPLVQIYELEDHKIQTWRGDSFQNVYLQFYNQSLISITPEASLFDAVYSLLKHKIHRLPVIDPESGNVLHILTHKRILKFLYIFRATIPTPRFLQRKIEEAGVGTFRDIATIQQTATVYDALSIFAERRVSALPVVDENGKVVALYSRFDVINLAAQKTYNNLSMSMKEALRRRRCFVEEVIKCYPDETLEDAVDRIADAEVHRLVLVDREDHTVRGIISLSDLLQAMILSPAGINALLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q6B5</accession>
    <name>A0A2D0Q6B5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Zona pellucida sperm-binding protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC108258640</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017312895.1">
        <property type="nucleotide sequence ID" value="XM_017457406.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q6B5"/>
    <dbReference type="GeneID" id="108258640"/>
    <dbReference type="KEGG" id="ipu:108258640"/>
    <dbReference type="OMA" id="VEQYLHC"/>
    <dbReference type="OrthoDB" id="8961289at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032190">
        <property type="term" value="F:acrosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035803">
        <property type="term" value="P:egg coat formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000344">
        <property type="term" value="P:positive regulation of acrosome reaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.4100:FF:000002">
        <property type="entry name" value="Zona pellucida sperm-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055356">
        <property type="entry name" value="ZP-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576:SF26">
        <property type="entry name" value="ZONA PELLUCIDA GLYCOPROTEIN 3D TANDEM DUPLICATE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23344">
        <property type="entry name" value="ZP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013130286" evidence="2">
        <location>
            <begin position="19"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="3">
        <location>
            <begin position="116"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017312895.1"/>
        </source>
    </evidence>
    <sequence length="444" mass="49877" checksum="3D85DBC97B212CD2" modified="2017-12-20" version="1">MLLKAVLLLVVRFCVVSPFPYMPDEAASDDSWPDLEESLPKYKAQTPVSPTPHTPCPPLKLPQFQQISSSDVHKLIFKPARNLRPVPALMKSILLPSPTPATAAPLPSKPRRIELLCHIDRIYVRVRKSLFTNPDAGKYLKVGTCTVNKDTAEYYYFLYPINSCTVQRHENENRVLYSNVLSYEPLTDEPVVRELPFSVPLECRYNKHFRSYNIGYHPQVEAGTVFLSLQGGVSLTPVDENWEPLGMLHSYTIGQPMYFVAMAPYGHAGTRLYLSKCYITASQNPDTTPNYTVMDNYGCMVDSKKTSQSKFHPSENKATLRFSVGALLFKDMVSQPENKTEMFIHCEMYLGPEIPTSSTKSCSYNADTQGWSELYGDDAVCACCDASCPAPEPSMSTRISSNAWMVEKGQNDQPASKSKTEVVEEAQSRGGFEMFWDVFDEVIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q811</accession>
    <name>A0A2D0Q811_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Cyclic AMP-responsive element-binding protein 3-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">creb3l1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the bZIP family. ATF subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017314823.1">
        <property type="nucleotide sequence ID" value="XM_017459334.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q811"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000001710"/>
    <dbReference type="GeneID" id="108259676"/>
    <dbReference type="KEGG" id="ipu:108259676"/>
    <dbReference type="CTD" id="90993"/>
    <dbReference type="OrthoDB" id="674948at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035497">
        <property type="term" value="F:cAMP response element binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14689">
        <property type="entry name" value="bZIP_CREB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000054">
        <property type="entry name" value="Cyclic AMP-responsive element-binding protein 3-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46004">
        <property type="entry name" value="CYCLIC AMP RESPONSE ELEMENT-BINDING PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46004:SF1">
        <property type="entry name" value="CYCLIC AMP-RESPONSIVE ELEMENT-BINDING PROTEIN 3-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0834">Unfolded protein response</keyword>
    <feature type="domain" description="BZIP" evidence="12">
        <location>
            <begin position="307"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="66"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="122"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="188"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="325"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="199"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="220"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017314823.1"/>
        </source>
    </evidence>
    <sequence length="533" mass="58845" checksum="466A44C230193450" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q8G6</accession>
    <name>A0A2D0Q8G6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Transmembrane protein 163 isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">tmem163b</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle</location>
            <location evidence="2">Synaptic vesicle membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TMEM163 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017314011.1">
        <property type="nucleotide sequence ID" value="XM_017458522.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q8G6"/>
    <dbReference type="GeneID" id="108259241"/>
    <dbReference type="CTD" id="100333673"/>
    <dbReference type="OrthoDB" id="5980560at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026765">
        <property type="entry name" value="Tmem163"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31937">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31937:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 11 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017314011.1"/>
        </source>
    </evidence>
    <sequence length="229" mass="25234" checksum="3601EDB791E7CBD9" modified="2017-12-20" version="1">MCERTFRDLLESSQRLKPHEAQSYRKKALWVSWVSIVVTLILAIAAFTVSTMRSSASAFGFAFDATLDVLSSIIVLWRYRNAAAVHSAHREYIACVVLGVVFILSGLTILIKAAHALATKVLPEVDDFLYSVSVLSGLVCSILAVLKCMLGKVLTSRALITDGFNSLVGGVMGFSILISAEVFKHNPKVWYLDGAIGVLIGLIILAYGVKLLIDMVPRVRQTRHYERFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QAL3</accession>
    <name>A0A2D0QAL3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Grass carp reovirus (GCRV)-induced gene 2p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gig2p</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017315329.1">
        <property type="nucleotide sequence ID" value="XM_017459840.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QAL3"/>
    <dbReference type="GeneID" id="108259973"/>
    <dbReference type="KEGG" id="ipu:108259973"/>
    <dbReference type="CTD" id="796385"/>
    <dbReference type="OrthoDB" id="9894570at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.175.10:FF:000003">
        <property type="entry name" value="Grass carp reovirus (GCRV)-induced gene 2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.175.10">
        <property type="entry name" value="Diphtheria Toxin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36542">
        <property type="entry name" value="GIG2-LIKE PROTEIN DRED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36542:SF6">
        <property type="entry name" value="GIG2-LIKE PROTEIN DREP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PARP catalytic" evidence="2">
        <location>
            <begin position="24"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017315329.1"/>
        </source>
    </evidence>
    <sequence length="216" mass="24396" checksum="4E7837C6D67C4426" modified="2017-12-20" version="1">MSVTFYGWEVDYDEDHHLRAEQAAKSGRHYTMYHGTNVQAARHIIQHGFRQSQDGMLGPGVYVSRNQKKAERYPLNSHVTDRVVLKLSVDCGKVKRIDKDNHPMQKTWHSNGYDTAWVPPRCGMKAVPSGLEEDCVWDPKRIEVTDIGLAPSTTILNELKQLLAQKSPQAAAAAANPQVCQLCKWKIVPEHNVQTCWGCGETICTLMTKHKCNQWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QD83</accession>
    <name>A0A2D0QD83_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Cationic amino acid transporter 2 family protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">zgc:175280</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-arginine(in) = L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:32143"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-lysine(in) = L-lysine(out)</text>
            <dbReference type="Rhea" id="RHEA:70935"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-ornithine(in) = L-ornithine(out)</text>
            <dbReference type="Rhea" id="RHEA:71199"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017315676.2">
        <property type="nucleotide sequence ID" value="XM_017460187.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QD83"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030589"/>
    <dbReference type="GeneID" id="108260143"/>
    <dbReference type="KEGG" id="ipu:108260143"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061459">
        <property type="term" value="F:L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015189">
        <property type="term" value="F:L-lysine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097638">
        <property type="term" value="P:L-arginine import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000024">
        <property type="entry name" value="High affinity cationic amino acid transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000009">
        <property type="entry name" value="Low affinity cationic amino acid transporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004755">
        <property type="entry name" value="Cat_AA_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029485">
        <property type="entry name" value="CAT_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00906">
        <property type="entry name" value="2A0303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243:SF19">
        <property type="entry name" value="CATIONIC AMINO ACID TRANSPORTER C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER YGJI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13906">
        <property type="entry name" value="AA_permease_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="167"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="289"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="338"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="411"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="461"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="490"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="522"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="555"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="Cationic amino acid transporter C-terminal" evidence="14">
        <location>
            <begin position="522"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017315676.2"/>
        </source>
    </evidence>
    <sequence length="608" mass="65973" checksum="0C7D9A2AAA4AD075" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QFA7</accession>
    <name>A0A2D0QFA7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ufm1-specific protease 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ufsp2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C78 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017316984.1">
        <property type="nucleotide sequence ID" value="XM_017461495.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QFA7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035021"/>
    <dbReference type="GeneID" id="108260847"/>
    <dbReference type="KEGG" id="ipu:108260847"/>
    <dbReference type="CTD" id="55325"/>
    <dbReference type="OMA" id="RTHCKET"/>
    <dbReference type="OrthoDB" id="417506at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071567">
        <property type="term" value="F:deUFMylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.130:FF:000001">
        <property type="entry name" value="Probable Ufm1-specific protease 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012462">
        <property type="entry name" value="UFSP1/2_DUB_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049387">
        <property type="entry name" value="UFSP2-like_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48153">
        <property type="entry name" value="UFM1-SPECIFIC PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48153:SF2">
        <property type="entry name" value="UFM1-SPECIFIC PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07910">
        <property type="entry name" value="Peptidase_C78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20908">
        <property type="entry name" value="UfSP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 11" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UFSP2 second" evidence="9">
        <location>
            <begin position="36"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="UFSP1/2/DUB catalytic" evidence="8">
        <location>
            <begin position="275"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017316984.1"/>
        </source>
    </evidence>
    <sequence length="466" mass="52484" checksum="B02EDFE8A3812577" modified="2017-12-20" version="1">MVLTENNNFIFRLKGVLEFSCQLDSSNEAHGQNAISKAFRVLRSRISSESLVFMVCNSPILLWPNKSCHSTVESLSERTPCRDVQQHIDLEDGPNRKSSKRRDKKDASPITVNLKLLMEVTESGSGGALSLCRVTKKQQCVMMPLCMDCVINAGGHDTLGFVCSGLVAALHAQLSDMEQTVLKYRKDKSLPLPEPFHFQLPASDRLFTVIYPAAVTDDQLLSVREELHRKHDLPRDRPYFRRANAFHFPGEPYKDGYLRSPHTHLNAPSIEDAKLYVVRGVYSYHHYMQDRVDDNGWGCAYRSLQTICSWFQQQGYTERTVPTHKQIQQALVDVGDKEPSFLGSRQWIGSIEVQAVLNQLLGVNSKILFVSRGSELASKGRELANHFQTEGTPIMIGGGVLAHTILGVAWSETSGQIRFLILDPHYTGGEDIQTITDKGWCGWKGPEFWDQNAYYNLCLPQTPKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QM36</accession>
    <name>A0A2D0QM36_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tetratricopeptide repeat protein 9A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC108262938</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the TTC9 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017318775.1">
        <property type="nucleotide sequence ID" value="XM_017463286.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QM36"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000004988"/>
    <dbReference type="GeneID" id="108262938"/>
    <dbReference type="KEGG" id="ipu:108262938"/>
    <dbReference type="OMA" id="RSSGCGQ"/>
    <dbReference type="OrthoDB" id="433738at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000264">
        <property type="entry name" value="Tetratricopeptide repeat protein 9A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039663">
        <property type="entry name" value="AIP/AIPL1/TTC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11242">
        <property type="entry name" value="ARYL HYDROCARBON RECEPTOR INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11242:SF15">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 9A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00073858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017318775.1"/>
        </source>
    </evidence>
    <sequence length="244" mass="28096" checksum="EC23172FBB131AF3" modified="2017-12-20" version="1">MMMSLDRSGCEGKIAPEGVTGSLRQPPPRAQLSAGVRAREIRSLQQHQQQNQHHHHHHLHHHHHHHHHAGGAREPDELLRRALDFKTQGTQCYRDKKYREAIGKYHRALLEMKGLGRALGFGDADVSARSPNRSGLTEEQRGAAESAELECYNSLAACLLQMELVNYERVKEYCLKVLRKEGENFKALYRSGVAYYHLGDYNSALHYLQESHKQEPTDTNVIRYIQLTEMKLRRIAQREKKDPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QP67</accession>
    <name>A0A2D0QP67_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Homeobox protein MSH-C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">msx3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the Msh homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017319531.1">
        <property type="nucleotide sequence ID" value="XM_017464042.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QP67"/>
    <dbReference type="GeneID" id="108263347"/>
    <dbReference type="KEGG" id="ipu:108263347"/>
    <dbReference type="CTD" id="17703"/>
    <dbReference type="OMA" id="MAPYPFA"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043049">
        <property type="term" value="P:otic placode formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000134">
        <property type="entry name" value="Homeobox protein MSX-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050674">
        <property type="entry name" value="Msh_Homeobox_Regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24338">
        <property type="entry name" value="HOMEOBOX PROTEIN MSX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24338:SF9">
        <property type="entry name" value="HOMEOBOX PROTEIN MSX-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="147"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="149"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="108"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="64"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="143"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017319531.1"/>
        </source>
    </evidence>
    <sequence length="271" mass="29728" checksum="2B036128AC1E6183" modified="2017-12-20" version="1">MAPLPATMSSVQGTISQEGEAQKRANTDAEEAESTERAAAKGCKNKAFSLPFSVESLISDRVTSRTSYSASDSGLISPSEERSQLSPMGLYTDRKLPLESVSNLAVGKRGDSEELNEKGQGGWFQTPSYASPPRHPSPSPCALRKHKNNRKPRTPFTTSQLLALERKFRQKQYLSIAERAEFSNSLNLTETQVKIWFQNRRAKAKRLQEAELEKLKLASKPLLPAFAFPFPLGAHVTSPGLYAASSSFPRPSLPVPGLFGGPVTYGMYYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QXM0</accession>
    <name>A0A2D0QXM0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">WD repeat-containing protein 13 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">wdr13</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017322496.1">
        <property type="nucleotide sequence ID" value="XM_017467007.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QXM0"/>
    <dbReference type="GeneID" id="108264979"/>
    <dbReference type="KEGG" id="ipu:108264979"/>
    <dbReference type="CTD" id="64743"/>
    <dbReference type="OMA" id="MLKFANN"/>
    <dbReference type="OrthoDB" id="1932312at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051350">
        <property type="entry name" value="WD_repeat-ST_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22838">
        <property type="entry name" value="WD REPEAT PROTEIN 26-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22838:SF4">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="210"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="446"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="75"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017322496.1"/>
        </source>
    </evidence>
    <sequence length="482" mass="53597" checksum="9B5EA531145F0044" modified="2017-12-20" version="1">MAAVWQQVLAVDARYNAYRTPTFPQFRTQYIRRRSQLLRENAKCGFEPVLRRQYLRLRSQLLALRYGPLSEQSSFRASSVRSSRTTLDRMEDFEEDSRAQGARGHRRSVSRGSYQLQAQMNRAVYEERPPGSLVPTSVAEASRAMAGDTTLSENYAFAGMHHIFDQHVDSAVPRLQFANDDKHLLACCSLDGTLSIMTLSPPPPAVRVTLRGHAGPVTDFAWSLSNDIIVSTSLDGTLRIWNTEDGRCIREVADPETSELLCCTFQPMNNNLTVVGNSKHLLQVVNISTGKKVKGGSSKLTGRVLSLSFDAPGRILWAGDDRGSIFSFLFDMATGKLTKAKRLVVSEGSPISSISARSWISREARDPSLLINACVNKLLLYRVVDNEGTLQLKRSFPIQHGSQHLHSIFCPLMSFRQGACIVTGSEDACVYFFDVERNTKAIVNKLQGHSGPVLDVSFNCDESLLASSDATGMVIIWRREQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QZE5</accession>
    <name>A0A2D0QZE5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">CMRF35-like molecule 9 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC108265688</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017323768.1">
        <property type="nucleotide sequence ID" value="XM_017468279.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QZE5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007303"/>
    <dbReference type="GeneID" id="108265688"/>
    <dbReference type="KEGG" id="ipu:108265688"/>
    <dbReference type="OrthoDB" id="9805957at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF118">
        <property type="entry name" value="CMRF35-LIKE MOLECULE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="237"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="5">
        <location>
            <begin position="81"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017323768.1"/>
        </source>
    </evidence>
    <sequence length="334" mass="36402" checksum="2FB2F7299D38A081" modified="2017-12-20" version="1">MDKSLLPPAYCEEPEVISQTSSAASCYIVQLTAQKRQNMYISHCAEFSKKAGDVQKIMQRFLWTLSLFQVLICMTMKVKAESVFTGTEGGSVDISCKYPAGYQYTPMYFCRDPCSYSDVLIKSVKADTVASKERYTALNTLSARRFSVTIRHLTLKDSGVYYCGVDTWGKDKLTKVKLTVSEAVPTVSTRAPVTSQNQIPEVTESPCATTVITAAAESSTFYEQASSTPQTQHSLDLYGQLFVVCGGVLGLMLCCVLAALVILYRKKSTHITTLKPAAPAIQISHPPPGQENICHVYDEMLAVYTLAGPPVGDESSATYSTIQLPAPADNDCSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R115</accession>
    <name>A0A2D0R115_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutaminase</fullName>
            <ecNumber evidence="2">3.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gls2a</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.2" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017324337.1">
        <property type="nucleotide sequence ID" value="XM_017468848.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R115"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003168"/>
    <dbReference type="GeneID" id="108265957"/>
    <dbReference type="KEGG" id="ipu:108265957"/>
    <dbReference type="CTD" id="100006303"/>
    <dbReference type="OrthoDB" id="9995210at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006543">
        <property type="term" value="P:L-glutamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.710.10:FF:000002">
        <property type="entry name" value="glutaminase kidney isoform, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000019">
        <property type="entry name" value="Glutaminase liver isoform, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00313">
        <property type="entry name" value="Glutaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015868">
        <property type="entry name" value="Glutaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041541">
        <property type="entry name" value="Glutaminase_EF-hand"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03814">
        <property type="entry name" value="Gln_ase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12544">
        <property type="entry name" value="GLUTAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12544:SF33">
        <property type="entry name" value="GLUTAMINASE LIVER ISOFORM, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17959">
        <property type="entry name" value="EF-hand_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04960">
        <property type="entry name" value="Glutaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Glutaminase EF-hand" evidence="8">
        <location>
            <begin position="72"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="517"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017324337.1"/>
        </source>
    </evidence>
    <sequence length="612" mass="67767" checksum="34318A9EDBB83C11" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R8I8</accession>
    <name>A0A2D0R8I8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Carcinoembryonic antigen-related cell adhesion molecule 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC108267009</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017326376.1">
        <property type="nucleotide sequence ID" value="XM_017470887.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R8I8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012226"/>
    <dbReference type="GeneID" id="108267009"/>
    <dbReference type="KEGG" id="ipu:108267009"/>
    <dbReference type="OrthoDB" id="10012075at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052598">
        <property type="entry name" value="IgSF_CEA-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44337:SF16">
        <property type="entry name" value="CARCINOEMBRYONIC ANTIGEN-RELATED CELL ADHESION MOLECULE 20-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44337">
        <property type="entry name" value="CARCINOEMBRYONIC ANTIGEN-RELATED CELL ADHESION MOLECULE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012994273" evidence="7">
        <location>
            <begin position="19"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="124"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="216"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="349"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="373"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="391"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017326376.1"/>
        </source>
    </evidence>
    <sequence length="400" mass="43631" checksum="1B32C29F2AC83A78" modified="2017-12-20" version="1">MEQAVVVTLIITLLTVSCEEGNLLPPGPINKEVGASVLFSTFPSSQQIRGIRWHFNGIEVTRYYFPGPITVHPDYNGRVNLNYTSGDLELKNLTMADSGQYTLTLTFESGLGNQDHILLQVFEPVFSFDITGPEGNLIEYSTVNFTCNGNGTISSTVWMKKYEILAPSSSITFLDDNRTMVISRVQRSDSGDYQCAISNPVSSSTATYSITVNYGPDVKILGAQTFEEGSDILLLCVPDSLPAATITWTVQGMPAKNASLYVIENSSRSNSGEYICTCWNNVTGIRASAVQSVTVRAVSSRLGPGVEAGITVGVIFAVVILVVLICFLVKHFKKPTDTVRETPVPMIVNQQTTVPTGDKLQGSRPHIYQNNQPSPPPRKPPTLPRSRDTNDSNIYCNTTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RCH1</accession>
    <name>A0A2D0RCH1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">psmb5</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Within the 20S core complex, PSMB5 displays a chymotrypsin-like activity.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="function">
        <text evidence="10">The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the proteasome complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7. Directly interacts with POMP. Interacts with ABCB1 and TAP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017328238.3">
        <property type="nucleotide sequence ID" value="XM_017472749.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RCH1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000010143"/>
    <dbReference type="GeneID" id="108268065"/>
    <dbReference type="KEGG" id="ipu:108268065"/>
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    <dbReference type="OrthoDB" id="37597at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03761">
        <property type="entry name" value="proteasome_beta_type_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000030">
        <property type="entry name" value="Proteasome subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF11">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="7 13 15" id="KW-0647">Proteasome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017328238.3"/>
        </source>
    </evidence>
    <sequence length="266" mass="29441" checksum="B21FEDE9766482B9" modified="2023-06-28" version="3">MALASVLKSECADFEQPFTFGCGFDDTAVGFDETSGDRLNFTLKRFEGEEDGIERKIEFLHGTTTLAFKFQHGVIVAVDSRATAGSYIASQTVKKVIEINPYLLGTMAGGAADCSFWERLLARQCRIYELRNKERISVAAASKLLANMVYQYKGMGLSMGTMVCGWDKRGPGLYYVDSEGNRVCGDMFAVGSGSMYAYGVLDSGHRTDLTIEEACDLGRRAIYQATFRDAYSGGQVNLYRVHSEGWERVSQDDVLALHHQFQSQKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RGL6</accession>
    <name>A0A2D0RGL6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Zinc finger protein 319</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">znf319b</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017329678.1">
        <property type="nucleotide sequence ID" value="XM_017474189.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RGL6"/>
    <dbReference type="GeneID" id="108268877"/>
    <dbReference type="KEGG" id="ipu:108268877"/>
    <dbReference type="CTD" id="792588"/>
    <dbReference type="OrthoDB" id="8895262at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001677">
        <property type="entry name" value="Zinc finger protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001049">
        <property type="entry name" value="zinc finger protein 319"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001217">
        <property type="entry name" value="zinc finger protein 319"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000184">
        <property type="entry name" value="Zinc finger protein 333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000202">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000788">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050758">
        <property type="entry name" value="Znf_C2H2-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234:SF10">
        <property type="entry name" value="RIKEN CDNA 6720489N17 GENE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234">
        <property type="entry name" value="ZNF44 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="224"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="252"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="280"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="308"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="336"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="364"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="394"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="422"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="456"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="484"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="512"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="540"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="568"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="43"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="177"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="43"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="177"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="195"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017329678.1"/>
        </source>
    </evidence>
    <sequence length="604" mass="67885" checksum="E75A4640B26EB3DC" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RJN1</accession>
    <name>A0A2D0RJN1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15 16">Atlastin-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15 16" type="primary">atl3</name>
    </gene>
    <organism evidence="14 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15 16" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15 16">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017330355.1">
        <property type="nucleotide sequence ID" value="XM_017474866.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017330356.1">
        <property type="nucleotide sequence ID" value="XM_017474867.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RJN1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032232"/>
    <dbReference type="GeneID" id="108269196"/>
    <dbReference type="KEGG" id="ipu:108269196"/>
    <dbReference type="CTD" id="25923"/>
    <dbReference type="GeneTree" id="ENSGT00940000158566"/>
    <dbReference type="OMA" id="QYQKNME"/>
    <dbReference type="OrthoDB" id="7788754at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098826">
        <property type="term" value="C:endoplasmic reticulum tubular network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990809">
        <property type="term" value="P:endoplasmic reticulum tubular network membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01851">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.420:FF:000001">
        <property type="entry name" value="Atlastin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000314">
        <property type="entry name" value="Atlastin-2 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.420">
        <property type="entry name" value="AHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003191">
        <property type="entry name" value="Guanylate-bd/ATL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036543">
        <property type="entry name" value="Guanylate-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015894">
        <property type="entry name" value="Guanylate-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10751">
        <property type="entry name" value="GUANYLATE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02263">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02841">
        <property type="entry name" value="GBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48340">
        <property type="entry name" value="Interferon-induced guanylate-binding protein 1 (GBP1), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="423"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="447"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="GB1/RHD3-type G" evidence="13">
        <location>
            <begin position="38"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017330355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017330356.1"/>
        </source>
    </evidence>
    <sequence length="511" mass="57451" checksum="D0B6235AECD7AB5A" modified="2017-12-20" version="1">MPTEPGAVQIVTVNKEDHSFDLDTKALEKILLRPQVRDMDVVVLSVAGAFRKGKSFLLDFMLRYMHRKPGQDWLGSDDEPLTGFSWRGGSEPETTGIQLWSEVFTVRKSNGTEVAVLLMDTQGAFDNQSTVKDCATIFALSTMTSSVQIYNLSQNIQEDDLQQLQLFTEYGRLAMDEIFLKPFQSLMFLIRDWSFPYEYSYGFKGGNQFLDKRLQVKESQHQELQTVRKHIHSCFTSISCFLLPHPGLKVATSPSFQGQLCDVAPEFKTQLCSLIPKLLDPDSLAVKEINGSKVTCRGLVECFKSYIKIYQGEDLPHPKSMLQATAEANNLTAVASAKDQYYKNMEKICGGDVPYVAPDSLLEKHNFFKCEALHHFSSIKKMGGKEFCAPYQVQLEAELNELWESFSKHNESKNLFNAFRTPAVLFVLVCLLYVLSALLLFIGLSSVSFTCDCMLGLAMVAVVTWAFIRFSGQYRHVGVAIDQVAGVLLEQATGMLNKSRTQAITQDKKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RQ52</accession>
    <name>A0A2D0RQ52_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">Multifunctional fusion protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="10">Synaptosomal-associated protein 25</fullName>
                <shortName evidence="10">SNAP-25</shortName>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="9">Synaptosomal-associated protein</fullName>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="14" type="primary">snap25b</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Plays an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Synapse</location>
            <location evidence="10">Synaptosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the SNAP-25 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017332296.1">
        <property type="nucleotide sequence ID" value="XM_017476807.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RQ52"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000023160"/>
    <dbReference type="GeneID" id="108270282"/>
    <dbReference type="KEGG" id="ipu:108270282"/>
    <dbReference type="CTD" id="30711"/>
    <dbReference type="OMA" id="GQAWGSN"/>
    <dbReference type="OrthoDB" id="19261at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070032">
        <property type="term" value="C:synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017075">
        <property type="term" value="F:syntaxin-1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045162">
        <property type="term" value="P:clustering of voltage-gated sodium channels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007626">
        <property type="term" value="P:locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031629">
        <property type="term" value="P:synaptic vesicle fusion to presynaptic active zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016082">
        <property type="term" value="P:synaptic vesicle priming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15885">
        <property type="entry name" value="SNARE_SNAP25C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15894">
        <property type="entry name" value="SNARE_SNAP25N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000007">
        <property type="entry name" value="Synaptosomal-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000009">
        <property type="entry name" value="Synaptosomal-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000928">
        <property type="entry name" value="SNAP-25_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039077">
        <property type="entry name" value="SNAP-25_N_SNARE_chord"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305">
        <property type="entry name" value="SYNAPTOSOMAL ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305:SF5">
        <property type="entry name" value="SYNAPTOSOMAL-ASSOCIATED PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00835">
        <property type="entry name" value="SNAP-25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 10" id="KW-0771">Synaptosome</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="19"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="137"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017332296.1"/>
        </source>
    </evidence>
    <sequence length="203" mass="23023" checksum="B675E2B8849EDEB2" modified="2017-12-20" version="1">MADENDMRNELTNLQERADQLTDDSLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGNLCGICPCPCNKLKERGQNWGNNQDGVVSSQPARVMDEREQMAISGGFIRRVTNDARENEMDENLEQVGSIIGNLRHMALDMGNEIDTQNRQIDRIMDMSDSNKTRIDEANQRATKMLGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RQM2</accession>
    <name>A0A2D0RQM2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Neurocan core protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Chondroitin sulfate proteoglycan 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">ncanb</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017332808.1">
        <property type="nucleotide sequence ID" value="XM_017477319.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RQM2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000815"/>
    <dbReference type="GeneID" id="108270525"/>
    <dbReference type="KEGG" id="ipu:108270525"/>
    <dbReference type="CTD" id="559024"/>
    <dbReference type="OMA" id="HESGHWN"/>
    <dbReference type="OrthoDB" id="5860362at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072534">
        <property type="term" value="C:perineuronal net"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005540">
        <property type="term" value="F:hyaluronic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010001">
        <property type="term" value="P:glial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002052">
        <property type="term" value="P:positive regulation of neuroblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd03517">
        <property type="entry name" value="Link_domain_CSPGs_modules_1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03520">
        <property type="entry name" value="Link_domain_CSPGs_modules_2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000011">
        <property type="entry name" value="Aggrecan core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000012">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000002">
        <property type="entry name" value="Hyaluronan proteoglycan link protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000571">
        <property type="entry name" value="Neurocan core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000003">
        <property type="entry name" value="Versican core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000003">
        <property type="entry name" value="Versican core protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000006">
        <property type="entry name" value="Versican core protein-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050691">
        <property type="entry name" value="Hyaluronan_bind_Proteoglycan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000538">
        <property type="entry name" value="Link_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22804">
        <property type="entry name" value="AGGRECAN/VERSICAN PROTEOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22804:SF24">
        <property type="entry name" value="NEUROCAN CORE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00193">
        <property type="entry name" value="Xlink"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01265">
        <property type="entry name" value="LINKMODULE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00445">
        <property type="entry name" value="LINK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01241">
        <property type="entry name" value="LINK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50963">
        <property type="entry name" value="LINK_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="11 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 17" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0430">Lectin</keyword>
    <keyword evidence="10" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 21" id="KW-0732">Signal</keyword>
    <keyword evidence="4 18" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043456049" description="Neurocan core protein" evidence="21">
        <location>
            <begin position="30"/>
            <end position="1273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="688"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="838"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="918"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1236"/>
            <end position="1273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="688"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="709"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="838"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="848"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="863"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="930"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="1236"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="995"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="1033"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="1176"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="1205"/>
            <end position="1232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00059308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00073685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017332808.1"/>
        </source>
    </evidence>
    <sequence length="1273" mass="141340" checksum="C4C8E05BA5F83F96" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RRG3</accession>
    <name>A0A2D0RRG3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycogen synthase kinase binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gbp</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSK-3-binding protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017333094.1">
        <property type="nucleotide sequence ID" value="XM_017477605.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RRG3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030762"/>
    <dbReference type="GeneID" id="108270737"/>
    <dbReference type="KEGG" id="ipu:108270737"/>
    <dbReference type="CTD" id="30719"/>
    <dbReference type="OrthoDB" id="6381246at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008014">
        <property type="entry name" value="GSK3-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35154">
        <property type="entry name" value="GBP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35154:SF3">
        <property type="entry name" value="GBP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05350">
        <property type="entry name" value="GSK-3_bind"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="52"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="52"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017333094.1"/>
        </source>
    </evidence>
    <sequence length="173" mass="19146" checksum="953E0425D5CFFD9E" modified="2017-12-20" version="1">MPCRKENYIFLEQSVTVDSKEVDALVTKIGEALQLHNNSATQQTMSRLHGLTTTTTTTSSSSSNNNNSSSSSNGKQSNNSNTASVQKRNGCCIRLRSGRKATRASPYNIPGSSDQEWDHFATWNRKGLDGTGNEDNPHQLLQELILSGNLIKEAVRRLQFSSESQRDFSKHID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S113</accession>
    <name>A0A2D0S113_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Selenocysteine-specific elongation factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Elongation factor sec</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Eukaryotic elongation factor, selenocysteine-tRNA-specific</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">eefsec</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Translation factor required for the incorporation of the rare amino acid selenocysteine encoded by UGA codons. Replaces the eRF1-eRF3-GTP ternary complex for the insertion of selenocysteine directed by the UGA codon. Insertion of selenocysteine at UGA codons is mediated by SECISBP2 and EEFSEC: SECISBP2 (1) specifically binds the SECIS sequence once the 80S ribosome encounters an in-frame UGA codon and (2) contacts the RPS27A/eS31 of the 40S ribosome before ribosome stalling. (3) GTP-bound EEFSEC then delivers selenocysteinyl-tRNA(Sec) to the 80S ribosome and adopts a preaccommodated state conformation. (4) After GTP hydrolysis, EEFSEC dissociates from the assembly, selenocysteinyl-tRNA(Sec) accommodates, and peptide bond synthesis and selenoprotein elongation occur.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017335900.1">
        <property type="nucleotide sequence ID" value="XM_017480411.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S113"/>
    <dbReference type="GeneID" id="108272165"/>
    <dbReference type="CTD" id="60678"/>
    <dbReference type="OMA" id="CFAIKGQ"/>
    <dbReference type="OrthoDB" id="2067at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001514">
        <property type="term" value="P:selenocysteine incorporation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04094">
        <property type="entry name" value="eSelB_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01889">
        <property type="entry name" value="SelB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03696">
        <property type="entry name" value="SelB_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000900">
        <property type="entry name" value="Eukaryotic elongation factor, selenocysteine-tRNA-specific"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000052">
        <property type="entry name" value="Selenocysteine-specific elongation factor EF-Sec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049394">
        <property type="entry name" value="eEFSec_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049393">
        <property type="entry name" value="eEFSec_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF11">
        <property type="entry name" value="SELENOCYSTEINE-SPECIFIC ELONGATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21131">
        <property type="entry name" value="eEFSec_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21208">
        <property type="entry name" value="euk_SelB_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
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        <property type="entry name" value="Translation proteins"/>
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    </dbReference>
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        <property type="entry name" value="G_TR_2"/>
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    </dbReference>
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    <keyword evidence="12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="18">
        <location>
            <begin position="15"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015953"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00054716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00076506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00082387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017335900.1"/>
        </source>
    </evidence>
    <sequence length="522" mass="56795" checksum="C81ADDB9D72C1D89" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S3L8</accession>
    <name>A0A2D0S3L8_ICTPU</name>
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        <submittedName>
            <fullName evidence="6 7">Dynein regulatory complex protein 11 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">iqca1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6 7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017337353.1">
        <property type="nucleotide sequence ID" value="XM_017481864.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053540306.1">
        <property type="nucleotide sequence ID" value="XM_053684331.1"/>
    </dbReference>
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    <dbReference type="STRING" id="7998.ENSIPUP00000017516"/>
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    <dbReference type="KEGG" id="ipu:108272963"/>
    <dbReference type="CTD" id="79781"/>
    <dbReference type="OrthoDB" id="3046016at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
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    <dbReference type="GO" id="GO:0005524">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000064">
        <property type="entry name" value="IQ motif containing with AAA domain 1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
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        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
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        <property type="entry name" value="N-DRC_Component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14690:SF11">
        <property type="entry name" value="IQ AND AAA DOMAIN-CONTAINING PROTEIN 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14690">
        <property type="entry name" value="IQ MOTIF CONTAINING WITH AAA DOMAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="699"/>
        </location>
    </feature>
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        <location>
            <begin position="241"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
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            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="457"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="821"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="254"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="361"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="463"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017337353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053540306.1"/>
        </source>
    </evidence>
    <sequence length="847" mass="97566" checksum="C8E6C95630735CEB" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S4B0</accession>
    <name>A0A2D0S4B0_ICTPU</name>
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        <submittedName>
            <fullName evidence="11">Thiamine transporter 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">slc19a2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the reduced folate carrier (RFC) transporter (TC 2.A.48) family.</text>
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    <dbReference type="RefSeq" id="XP_017337594.1">
        <property type="nucleotide sequence ID" value="XM_017482105.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S4B0"/>
    <dbReference type="GeneID" id="108273095"/>
    <dbReference type="KEGG" id="ipu:108273095"/>
    <dbReference type="CTD" id="10560"/>
    <dbReference type="OMA" id="CYRCRPL"/>
    <dbReference type="OrthoDB" id="18814at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090482">
        <property type="term" value="F:vitamin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000225">
        <property type="entry name" value="Solute carrier family 19 member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002666">
        <property type="entry name" value="Folate_carrier"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00806">
        <property type="entry name" value="rfc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10686">
        <property type="entry name" value="FOLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10686:SF19">
        <property type="entry name" value="THIAMINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01770">
        <property type="entry name" value="Folate_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028739">
        <property type="entry name" value="Folate_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="259"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="349"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="419"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="450"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR028739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017337594.1"/>
        </source>
    </evidence>
    <sequence length="478" mass="53119" checksum="5A10DB8BFB4EE54C" modified="2017-12-20" version="1">MPETWFYPTVLLCIYGFFSSLRPSEPFLTAFLMGPDKNLTEREVVNEIYPVWTYSYLILLFPVFLATDYLRYKPVLILQAASYIITYAMLLKAQGVLAIQVLEFFYGLATATEIAYYSYIYSVVEPSSYQKVTGFCRSATLLGSAVGSLIGQILFSEVHVSLLTLSIITLSSSCLAFLAPWFLPMPSKSLFFHQRERERQDNTAQELADKSEDHQSKVPLGVNEDSVILKERRGGTGGLVGVLKTLGADFVKCYSCRTLLAWSVWWALTTCGYFQVVNYAQALWENILSSKDFVIYNGYVETISTLLGAFAAFSVGLVKVSWAVWGELALCVFSIVIALSVYVMDTVRNIWVCYTSYVIFRTAYMLLITIATYQIAANLSMKRYALVFGVNTFVALLLQTLLTVIVVDPVGLGLDLFPQFLIYGSYFAAIAVIFLIAGLYKVTTRSSAAAPEVQSESAESETDPSGAETPSPKDNSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S597</accession>
    <name>A0A2D0S597_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Leucine-rich repeat-containing protein 69</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">lrrc69</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017337934.1">
        <property type="nucleotide sequence ID" value="XM_017482445.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S597"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019057"/>
    <dbReference type="GeneID" id="108273287"/>
    <dbReference type="KEGG" id="ipu:108273287"/>
    <dbReference type="CTD" id="100130742"/>
    <dbReference type="OMA" id="CFNKSGH"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
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        <property type="entry name" value="LRR_dom_sf"/>
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    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017337934.1"/>
        </source>
    </evidence>
    <sequence length="346" mass="39311" checksum="E17968914B5AC30E" modified="2017-12-20" version="1">MANDLVIRALKANSKSLNLSSRNITELSKCFAKLTRVLGLRLNNNRLTTLPLGLQGMRQLTELNLGNNAFEEVPPVLKHLHSLKKLYLFRNQISTLHPEVLEGLSNLIVLNLNSNKIKTIPTAIKSLLNLERFSIADNQLQEIPAELGLVSKLTEMNLTRNKLSEIPQELYKLTHLRKLSLARNSLRELPEGILGWKNLKMLDVAGNHLSMFPVDFHVLELEELYFEGNNLVRFELLTSAQEEEVLSLKEHAARFILKEHLNKSPVASRASLLLPDIETMLSRFGRCAICFEPFLTTWVECVQFINLRKDMGIKNSQNIPVRVLLCSYSCFNKSGHSYYSVAKVKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SA79</accession>
    <name>A0A2D0SA79_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Collagen, type I, alpha 1b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">col1a1b</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017339145.1">
        <property type="nucleotide sequence ID" value="XM_017483656.3"/>
    </dbReference>
    <dbReference type="GeneID" id="108273947"/>
    <dbReference type="KEGG" id="ipu:108273947"/>
    <dbReference type="CTD" id="325675"/>
    <dbReference type="OrthoDB" id="8939548at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1000:FF:000001">
        <property type="entry name" value="Collagen alpha-1 type I chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000013">
        <property type="entry name" value="Collagen, type I, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000885">
        <property type="entry name" value="Fib_collagen_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF1082">
        <property type="entry name" value="COLLAGEN TRIPLE HELIX REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01410">
        <property type="entry name" value="COLFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00093">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00038">
        <property type="entry name" value="COLFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51461">
        <property type="entry name" value="NC1_FIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 17" id="KW-0176">Collagen</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013288395" evidence="13">
        <location>
            <begin position="23"/>
            <end position="1449"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="14">
        <location>
            <begin position="31"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrillar collagen NC1" evidence="15">
        <location>
            <begin position="1214"/>
            <end position="1449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="95"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="130"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="175"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="193"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="217"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="236"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="306"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="324"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="361"/>
            <end position="406"/>
        </location>
    </feature>
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                <person name="Bao L."/>
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                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
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                <person name="Yuan Z."/>
                <person name="Yang Y."/>
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                <person name="Peatman E."/>
                <person name="Lu J."/>
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                <person name="Dunham R."/>
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                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
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                <person name="Kucuktas H."/>
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                <person name="Kirby M.L."/>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
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    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="nucleotide sequence ID" value="XM_017488482.3"/>
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    <dbReference type="OrthoDB" id="10040649at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
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        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:peptidase inhibitor activity"/>
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    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
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    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Elafin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
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        <property type="entry name" value="EGF"/>
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    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
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        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
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    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
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    <dbReference type="InterPro" id="IPR036645">
        <property type="entry name" value="Elafin-like_sf"/>
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        <property type="entry name" value="EMI_domain"/>
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    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
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    <dbReference type="InterPro" id="IPR000082">
        <property type="entry name" value="SEA_dom"/>
    </dbReference>
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        <property type="entry name" value="SEA_dom_sf"/>
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        <property type="entry name" value="WAP_dom"/>
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        <property type="entry name" value="ZP-C"/>
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    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
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        <property type="entry name" value="ZP_dom"/>
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        <property type="entry name" value="ENDOGLIN/TGF-BETA RECEPTOR TYPE III"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR14002:SF22">
        <property type="entry name" value="UROMODULIN-LIKE 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82671">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039912482" evidence="8">
        <location>
            <begin position="20"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="955"/>
            <end position="978"/>
        </location>
    </feature>
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        <location>
            <begin position="33"/>
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    </feature>
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        <location>
            <begin position="114"/>
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        <location>
            <begin position="259"/>
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        <location>
            <begin position="392"/>
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    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="503"/>
            <end position="541"/>
        </location>
    </feature>
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        <location>
            <begin position="679"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="546"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="580"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="549"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="580"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="612"/>
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        </location>
    </feature>
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        <location>
            <begin position="630"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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        <source>
            <dbReference type="PROSITE" id="PS50024"/>
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        <source>
            <dbReference type="PROSITE" id="PS50026"/>
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        <source>
            <dbReference type="PROSITE" id="PS51034"/>
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        <source>
            <dbReference type="PROSITE" id="PS51041"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51390"/>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017343971.2"/>
        </source>
    </evidence>
    <sequence length="1003" mass="109696" checksum="709E03D0A0D1B3E6" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SQF8</accession>
    <name>A0A2D0SQF8_ICTPU</name>
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        <submittedName>
            <fullName evidence="16">Mucin-2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC108277084</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
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    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
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    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.</text>
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        <property type="component" value="Chromosome 16"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Adhesion G protein-coupled receptor F5"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057400">
        <property type="entry name" value="ADGRF3/5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051587">
        <property type="entry name" value="Adhesion_GPCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
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        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
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    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
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    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
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    <dbReference type="InterPro" id="IPR000082">
        <property type="entry name" value="SEA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036364">
        <property type="entry name" value="SEA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45813:SF4">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR F5"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45813">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
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    <dbReference type="Pfam" id="PF25387">
        <property type="entry name" value="ADGRF3_N"/>
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        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
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        <property type="entry name" value="IG"/>
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        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82671">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GAIN_B"/>
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    <dbReference type="PROSITE" id="PS50024">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039887203" evidence="10">
        <location>
            <begin position="28"/>
            <end position="3495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="3156"/>
            <end position="3175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="3196"/>
            <end position="3217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="3237"/>
            <end position="3255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="3267"/>
            <end position="3294"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="3314"/>
            <end position="3338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="3359"/>
            <end position="3381"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="3387"/>
            <end position="3411"/>
        </location>
    </feature>
    <feature type="domain" description="SEA" evidence="11">
        <location>
            <begin position="2549"/>
            <end position="2656"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="2667"/>
            <end position="2730"/>
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    </feature>
    <feature type="domain" description="GAIN-B" evidence="12">
        <location>
            <begin position="2969"/>
            <end position="3147"/>
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    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="13">
        <location>
            <begin position="3154"/>
            <end position="3412"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="2376"/>
            <end position="2406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="2421"/>
            <end position="2527"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00007343"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50024"/>
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    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
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    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
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    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017344932.2"/>
        </source>
    </evidence>
    <sequence length="3495" mass="365003" checksum="FE1CC5C3F0437A9D" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SRV8</accession>
    <name>A0A2D0SRV8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22 23" type="primary">LOC108277322</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22 23" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22 23">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017345432.1">
        <property type="nucleotide sequence ID" value="XM_017489943.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047016736.1">
        <property type="nucleotide sequence ID" value="XM_047160780.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SRV8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005017"/>
    <dbReference type="GeneID" id="108277322"/>
    <dbReference type="KEGG" id="ipu:108277322"/>
    <dbReference type="GeneTree" id="ENSGT01030000234517"/>
    <dbReference type="OrthoDB" id="2156623at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000014">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000017">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF79">
        <property type="entry name" value="LOW QUALITY PROTEIN: MYOTONIN-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14 18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 22 23" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="84"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="20">
        <location>
            <begin position="350"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="479"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017345432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_047016736.1"/>
        </source>
    </evidence>
    <sequence length="602" mass="68479" checksum="281D097A8D4DAF76" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SWS0</accession>
    <name>A0A2D0SWS0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">plus-end-directed kinesin ATPase</fullName>
            <ecNumber evidence="17">5.6.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">kif1ab</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">SDA103</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O + a kinesin associated with a microtubule at position (n) = ADP + phosphate a kinesin associated with a microtubule at position (n+1, toward the plus end).</text>
            <dbReference type="EC" id="5.6.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.3" evidence="17"/>
    <dbReference type="RefSeq" id="XP_017346760.2">
        <property type="nucleotide sequence ID" value="XM_017491271.3"/>
    </dbReference>
    <dbReference type="GeneID" id="108278106"/>
    <dbReference type="KEGG" id="ipu:108278106"/>
    <dbReference type="CTD" id="566766"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010970">
        <property type="term" value="P:transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01365">
        <property type="entry name" value="KISc_KIF1A_KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01233">
        <property type="entry name" value="PH_KIFIA_KIFIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000023">
        <property type="entry name" value="Kinesin family member 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000001">
        <property type="entry name" value="Kinesin family member 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000004">
        <property type="entry name" value="Kinesin-like protein isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022164">
        <property type="entry name" value="Kinesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022140">
        <property type="entry name" value="Kinesin-like_KIF1-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032405">
        <property type="entry name" value="Kinesin_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049780">
        <property type="entry name" value="PH_KIFIA_KIFIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117:SF2">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF1A ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12473">
        <property type="entry name" value="DUF3694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12423">
        <property type="entry name" value="KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16183">
        <property type="entry name" value="Kinesin_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11 18" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="Kinesin motor" evidence="23">
        <location>
            <begin position="5"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="22">
        <location>
            <begin position="516"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="21">
        <location>
            <begin position="1637"/>
            <end position="1735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="861"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1502"/>
            <end position="1522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1597"/>
            <end position="1617"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="430"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="628"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="96"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00066390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_017346760.2"/>
        </source>
    </evidence>
    <sequence length="1752" mass="198162" checksum="E91D6E9346C19699" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SX79</accession>
    <name>A0A2D0SX79_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dual-specificity kinase</fullName>
            <ecNumber evidence="3">2.7.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">dyrk1ab</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017346925.1">
        <property type="nucleotide sequence ID" value="XM_017491436.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SX79"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021563"/>
    <dbReference type="GeneID" id="108278223"/>
    <dbReference type="KEGG" id="ipu:108278223"/>
    <dbReference type="CTD" id="327466"/>
    <dbReference type="OrthoDB" id="9332038at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14226">
        <property type="entry name" value="PKc_DYRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000087">
        <property type="entry name" value="Dual specificity tyrosine-phosphorylation-regulated kinase 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000264">
        <property type="entry name" value="dual specificity tyrosine-phosphorylation-regulated kinase 1B isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044131">
        <property type="entry name" value="PKc_DYR1A/1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF129">
        <property type="entry name" value="DUAL-SPECIFICITY KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="121"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="445"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="556"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="608"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="695"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="446"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="465"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="558"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="608"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017346925.1"/>
        </source>
    </evidence>
    <sequence length="715" mass="79915" checksum="EAE770907E09161B" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SXC9</accession>
    <name>A0A2D0SXC9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Alpha-2,8-sialyltransferase 8E</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Sialyltransferase 8E</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Sialyltransferase St8Sia V</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">st8sia5</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Involved in the synthesis of gangliosides GD1c, GT1a, GQ1b, GP1c and GT3 from GD1a, GT1b, GM1b and GD3 respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a ganglioside GQ1c (d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = a ganglioside GP1c (d18:1(4E)) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47592"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:87791"/>
            <dbReference type="ChEBI" id="CHEBI:87792"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:47593"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347339.1">
        <property type="nucleotide sequence ID" value="XM_017491850.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SXC9"/>
    <dbReference type="GeneID" id="108278458"/>
    <dbReference type="KEGG" id="ipu:108278458"/>
    <dbReference type="CTD" id="29906"/>
    <dbReference type="OrthoDB" id="10264956at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003828">
        <property type="term" value="F:alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23990">
        <property type="entry name" value="GT29_ST8SIA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1480.20:FF:000004">
        <property type="entry name" value="alpha-2,8-sialyltransferase 8E isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050943">
        <property type="entry name" value="Glycosyltr_29_Sialyltrsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012163">
        <property type="entry name" value="Sialyl_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987">
        <property type="entry name" value="ALPHA-2,8-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987:SF4">
        <property type="entry name" value="ALPHA-2,8-SIALYLTRANSFERASE 8E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005557">
        <property type="entry name" value="Sialyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="248"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00056957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00070573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00082846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR005557-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017347339.1"/>
        </source>
    </evidence>
    <sequence length="460" mass="52474" checksum="26A83D898FE0466F" modified="2017-12-20" version="1">MNAPLRYSPLLPPRGRMGYSDPTAGRDLLGNRSLCFIFICAFGVVTLLQQILYGKNYIRSTQQFTRLKGDEVGNWSGLVSYPDDGGMKLPARIGRKRCVRQNFQHSERSVVIVPCLADIKKKEKEKRRYLESSNGPLLYNTTTCRELRQEITDIKVLSMVKTSELFERWRNLQACSWAQNKEETSNFKMSLSRCCNAPSFLFTTQRNTPSGTKLRYEVDTSGILHISPEIFKMFPEDMPYSKSQFKKCAVIGNGGIIKNSKCGKEIDSADFVFRCNIPPISEKYAADVGTKTDLVTINPSIITERFQKLEKWRRPFYEVLASYENASVVLPAFYNARNTDVSFRVKYALDDFSGSAARGVFFFHPQYLLGAQRFWAAQGVHAKRLSSGLMLVTAALELCQEVHLYGFWAFPMNPAGIFVTHHYYDNVKPRPGFHAMPHEIFNFLHMHARGILHVHTGMCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SXH2</accession>
    <name>A0A2D0SXH2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Phosphatidylinositol-binding clathrin assembly protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LOC108278489</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly. Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature. In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8. In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors. Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF).</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Binds to clathrin; involves primarily the C-terminal sequences, but the full-length protein is required for full binding capacity. Binds phosphatidylinositol 4,5- bisphosphate. Interacts with PIMREG; this interaction may change the subcellular location into the nucleus. Interacts with AP2A1 (via its alpha-appendage domain). Interacts (via N-terminus) with VAMP2; VAMP3; VAMP7 and VAMP8 (Via N-terminus). Interacts with LC3/MAP1LC3A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <location evidence="5">Clathrin-coated pit</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PICALM/SNAP91 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347384.1">
        <property type="nucleotide sequence ID" value="XM_017491895.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SXH2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000036118"/>
    <dbReference type="GeneID" id="108278489"/>
    <dbReference type="KEGG" id="ipu:108278489"/>
    <dbReference type="OMA" id="THNGMIY"/>
    <dbReference type="OrthoDB" id="44015at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098894">
        <property type="term" value="C:extrinsic component of presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016185">
        <property type="term" value="P:synaptic vesicle budding from presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16985">
        <property type="entry name" value="ANTH_N_AP180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.150:FF:000001">
        <property type="entry name" value="phosphatidylinositol-binding clathrin assembly protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000001">
        <property type="entry name" value="phosphatidylinositol-binding clathrin assembly protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF11">
        <property type="entry name" value="ENTH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0168">Coated pit</keyword>
    <keyword evidence="17" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="ENTH" evidence="22">
        <location>
            <begin position="14"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="295"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="326"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00055144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00061829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00068054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_017347384.1"/>
        </source>
    </evidence>
    <sequence length="558" mass="61503" checksum="EFE6124EE3C826A5" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SXK4</accession>
    <name>A0A2D0SXK4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="12">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ppp3ca</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PPP phosphatase family. PP-2B subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="12"/>
    <dbReference type="RefSeq" id="XP_017347412.1">
        <property type="nucleotide sequence ID" value="XM_017491923.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SXK4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034954"/>
    <dbReference type="GeneID" id="108278499"/>
    <dbReference type="KEGG" id="ipu:108278499"/>
    <dbReference type="CTD" id="5530"/>
    <dbReference type="OrthoDB" id="5593063at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033192">
        <property type="term" value="F:calmodulin-dependent protein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097720">
        <property type="term" value="P:calcineurin-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07416">
        <property type="entry name" value="MPP_PP2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000002">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041751">
        <property type="entry name" value="MPP_PP2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043360">
        <property type="entry name" value="PP2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45673">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2B CATALYTIC SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="14">
        <location>
            <begin position="144"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="486"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="486"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="508"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017347412.1"/>
        </source>
    </evidence>
    <sequence length="520" mass="58728" checksum="7CEA49D4A1A4B7C1" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SXN8</accession>
    <name>A0A2D0SXN8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Enteropeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">tmprss15</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DMBT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347442.1">
        <property type="nucleotide sequence ID" value="XM_017491953.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SXN8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026868"/>
    <dbReference type="GeneID" id="108278517"/>
    <dbReference type="KEGG" id="ipu:108278517"/>
    <dbReference type="CTD" id="5651"/>
    <dbReference type="OrthoDB" id="425190at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009566">
        <property type="term" value="P:fertilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000128">
        <property type="entry name" value="enteropeptidase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000005">
        <property type="entry name" value="Procollagen C-endopeptidase enhancer 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.960">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000082">
        <property type="entry name" value="SEA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036364">
        <property type="entry name" value="SEA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Trypsin_dom"/>
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        <property type="entry name" value="TRYPSIN_HIS"/>
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    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
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    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF16">
        <property type="entry name" value="TRANSMEMBRANE SERINE PROTEASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Trypsin"/>
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    </dbReference>
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        <property type="entry name" value="CHYMOTRYPSIN"/>
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        <property type="match status" value="2"/>
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    </dbReference>
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        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="2"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TRYPSIN_DOM"/>
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        <property type="entry name" value="TRYPSIN_HIS"/>
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    </dbReference>
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        <property type="entry name" value="TRYPSIN_SER"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 15" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="16"/>
            <end position="40"/>
        </location>
    </feature>
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        <location>
            <begin position="51"/>
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            <begin position="218"/>
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            <begin position="338"/>
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        <location>
            <begin position="800"/>
            <end position="1035"/>
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    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="636"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="643"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="655"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="750"/>
            <end position="760"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
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        <source>
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        <source>
            <dbReference type="RefSeq" id="XP_017347442.1"/>
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    <sequence length="1044" mass="114632" checksum="2EB58A92D0081C69" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SY30</accession>
    <name>A0A2D0SY30_ICTPU</name>
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        <submittedName>
            <fullName evidence="8">RNA binding motif protein 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rbm11</name>
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        <name type="scientific">Ictalurus punctatus</name>
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        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
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        <property type="component" value="Chromosome 18"/>
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        <property type="term" value="C:nucleoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:single-stranded RNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="NEXT_complex_subunit"/>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13798">
        <property type="entry name" value="RNA BINDING MOTIF RBM PROTEIN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13798:SF11">
        <property type="entry name" value="RNA-BINDING PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="10"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="146"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="146"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017347588.1"/>
        </source>
    </evidence>
    <sequence length="184" mass="21180" checksum="BA013D9DE38B428F" modified="2017-12-20" version="1">MQESEGEEQHTVFVFNLSNCVREEILYELFLQAGPVRKVVIPKDRDGNQKSYGFVYYKHAEAVPYAIALLDGIWLYGCPITLRHHYVHTLTAQDDHSWRDATCALRGVPSMCPMQGPYFPPVIPWAWPKGGFVSWTSCSDELTQASDCTNSEEMGAEQNTEMENHRKGRRGHERRNKPKKKYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SYE7</accession>
    <name>A0A2D0SYE7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Coiled-coil domain-containing protein 80</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ccdc80l2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017347360.1">
        <property type="nucleotide sequence ID" value="XM_017491871.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SYE7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006027"/>
    <dbReference type="GeneID" id="108278473"/>
    <dbReference type="KEGG" id="ipu:108278473"/>
    <dbReference type="CTD" id="796417"/>
    <dbReference type="OrthoDB" id="9941497at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010811">
        <property type="term" value="P:positive regulation of cell-substrate adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025232">
        <property type="entry name" value="DUF4174"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46792:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING 80-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46792">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13778">
        <property type="entry name" value="DUF4174"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013175179" evidence="3">
        <location>
            <begin position="23"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4174" evidence="4">
        <location>
            <begin position="62"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4174" evidence="4">
        <location>
            <begin position="374"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4174" evidence="4">
        <location>
            <begin position="533"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="23"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="261"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="288"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="326"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="337"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="359"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017347360.1"/>
        </source>
    </evidence>
    <sequence length="694" mass="78033" checksum="E609E7448F2D596B" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SYK8</accession>
    <name>A0A2D0SYK8_ICTPU</name>
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        <submittedName>
            <fullName evidence="10 11 12">Angiomotin isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 11 12 13 14 15 16 17" type="primary">amot</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11 12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11 12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the angiomotin family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017492262.3"/>
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    <dbReference type="RefSeq" id="XP_017347752.1">
        <property type="nucleotide sequence ID" value="XM_017492263.3"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_017492264.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017347754.1">
        <property type="nucleotide sequence ID" value="XM_017492265.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017347755.1">
        <property type="nucleotide sequence ID" value="XM_017492266.3"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_017492267.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017347757.1">
        <property type="nucleotide sequence ID" value="XM_017492268.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017347758.1">
        <property type="nucleotide sequence ID" value="XM_017492269.3"/>
    </dbReference>
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    <dbReference type="KEGG" id="ipu:108278743"/>
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    <dbReference type="GeneTree" id="ENSGT00940000159055"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
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        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043534">
        <property type="term" value="P:blood vessel endothelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003365">
        <property type="term" value="P:establishment of cell polarity involved in ameboidal cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035329">
        <property type="term" value="P:hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009114">
        <property type="entry name" value="Angiomotin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051747">
        <property type="entry name" value="Angiomotin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024646">
        <property type="entry name" value="Angiomotin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14826">
        <property type="entry name" value="ANGIOMOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14826:SF6">
        <property type="entry name" value="ANGIOMOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12240">
        <property type="entry name" value="Angiomotin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01807">
        <property type="entry name" value="ANGIOMOTIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Angiomotin C-terminal" evidence="8">
        <location>
            <begin position="657"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="94"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="260"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="828"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="898"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="489"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="309"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="362"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="456"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="927"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="990"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1010"/>
            <end position="1019"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017347751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017347752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017347753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017347754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017347755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017347756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017347757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017347758.1"/>
        </source>
    </evidence>
    <sequence length="1019" mass="113319" checksum="969FF129B09B7B87" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T4F0</accession>
    <name>A0A2D0T4F0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Bromodomain-containing protein 1 isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">brd1a</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
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        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
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    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
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    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF102">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01462">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012135557" evidence="15">
        <location>
            <begin position="31"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="535"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="347"/>
            <end position="435"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="709"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="718"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017351439.1"/>
        </source>
    </evidence>
    <sequence length="728" mass="80378" checksum="A1E322866A63A0D0" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979E4I8</accession>
    <name>A0A979E4I8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Polymerase delta-interacting protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">poldip2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in DNA damage tolerance by regulating translesion synthesis (TLS) of templates carrying DNA damage lesions such as 8oxoG and abasic sites. May act by stimulating activity of DNA polymerases involved in TLS, such as PRIMPOL and polymerase delta (POLD1).</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with PCNA and POLD2. Interacts with SSBP1. Interacts with PRIMPOL; leading to enhance DNA polymerase activity of PRIMPOL. Interacts with POLH. Interacts with POLD1; leading to stimulate DNA polymerase activity of POLD1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="component" value="Chromosome 28"/>
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    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1470:FF:000001">
        <property type="entry name" value="DNA polymerase delta-interacting protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1470">
        <property type="entry name" value="ApaG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007474">
        <property type="entry name" value="ApaG_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036767">
        <property type="entry name" value="ApaG_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011722">
        <property type="entry name" value="Hemimethylated_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036623">
        <property type="entry name" value="Hemimethylated_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003967">
        <property type="entry name" value="PRK05461.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14289">
        <property type="entry name" value="F-BOX ONLY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14289:SF16">
        <property type="entry name" value="POLYMERASE DELTA-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04379">
        <property type="entry name" value="DUF525"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08755">
        <property type="entry name" value="YccV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00992">
        <property type="entry name" value="YccV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110069">
        <property type="entry name" value="ApaG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141255">
        <property type="entry name" value="YccV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51087">
        <property type="entry name" value="APAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="ApaG" evidence="12">
        <location>
            <begin position="243"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00073116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_047007767.1"/>
        </source>
    </evidence>
    <sequence length="381" mass="43879" checksum="BE3F151F2C52FD48" modified="2023-02-22" version="1">MAACVLRSWGILSTVNKFSNKHILRVLGVELNSRHEVNRRRLQCSTCGLLNVQQKRFMSSRNRAEGKVLETVGVFEAPKQNGKYETGQLFLHSVFGYRGIVLFPWHARLYDRDVAPPVSDSKPETPGAHGSKEVKGKTHTYYQVLIDTRDCPHISQRSQTEAVTFLANHDDSRALYAIPGLDYVSHEDILPYNSTDQVPIQHELFERFLMFNPSKVPPFVARDTLCAWQEKNHPWLELSDVHRETTENIRVTVIPFYMGMREAQNSHVYWWRYCIRLENMGSEVVQLRERHWRIFSLSGTLETVRGRGVVGREPVLSKEQPAFQYSSHVSLQAPSGHMWGSYRFERHNGTFFDVRIPPFSLESKKDDMPNSFLPGPFSSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EJA8</accession>
    <name>A0A979EJA8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="24">Chromodomain-helicase-DNA-binding protein 5 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">chd5</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
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    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
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        <property type="nucleotide sequence ID" value="XM_047149554.2"/>
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        <property type="component" value="Chromosome 21"/>
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        <property type="term" value="C:NuRD complex"/>
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        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
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        <property type="term" value="F:histone binding"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="entry name" value="PHD1_CHD_II"/>
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    </dbReference>
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        <property type="entry name" value="PHD2_CHD_II"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="CHD_C2"/>
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        <property type="entry name" value="CHD_II_SANT-like"/>
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        <property type="entry name" value="CHD_N"/>
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        <property type="entry name" value="Chromo-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
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    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
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    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
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        <property type="entry name" value="DUF1087"/>
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    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
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        <property type="entry name" value="Helicase_C-like"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="SNF2-like_sf"/>
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        <property type="entry name" value="SNF2_N"/>
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        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
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        <property type="entry name" value="Znf_FYVE_PHD"/>
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        <property type="entry name" value="Znf_PHD"/>
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        <property type="entry name" value="Znf_PHD-finger"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
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        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 5"/>
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        <property type="entry name" value="CHDCT2"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="CHROMO"/>
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        <property type="entry name" value="DEXDc"/>
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        <property type="entry name" value="DUF1086"/>
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        <property type="match status" value="2"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 17" id="KW-0863">Zinc-finger</keyword>
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    </feature>
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        <location>
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    <feature type="domain" description="Chromo" evidence="19">
        <location>
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    </feature>
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        <location>
            <begin position="713"/>
            <end position="897"/>
        </location>
    </feature>
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        <location>
            <begin position="1029"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="237"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="313"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="550"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1316"/>
            <end position="1375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1506"/>
            <end position="1691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1949"/>
            <end position="1990"/>
        </location>
    </feature>
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        <location>
            <begin position="7"/>
            <end position="28"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="72"/>
            <end position="89"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="99"/>
            <end position="117"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="256"/>
            <end position="289"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="321"/>
            <end position="333"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="1321"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="1342"/>
            <end position="1351"/>
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    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="1513"/>
            <end position="1527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1540"/>
            <end position="1594"/>
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    </feature>
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        <location>
            <begin position="1601"/>
            <end position="1626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1627"/>
            <end position="1666"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1674"/>
            <end position="1691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1960"/>
            <end position="1970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="1972"/>
            <end position="1990"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
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    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
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    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
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    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
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    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_047005510.1"/>
        </source>
    </evidence>
    <sequence length="1990" mass="226772" checksum="C28543A10B85E136" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979ELT4</accession>
    <name>A0A979ELT4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Centrosomal protein 43</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">FGFR1 oncogene partner</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">cep43</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Required for anchoring microtubules to the centrosomes. Required for ciliation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer. Part of a ternary complex that contains CEP350, CEP43 and MAPRE1. Interacts directly with CEP350 and MAPRE1. Interacts with CEP19. Interacts (via N-terminus) with CEP350 (via C-terminus).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CEP43 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047006850.1">
        <property type="nucleotide sequence ID" value="XM_047150894.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979ELT4"/>
    <dbReference type="GeneID" id="108257781"/>
    <dbReference type="CTD" id="11116"/>
    <dbReference type="OrthoDB" id="2160638at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034453">
        <property type="term" value="P:microtubule anchoring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018993">
        <property type="entry name" value="FOP_dimerisation-dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15431:SF9">
        <property type="entry name" value="CENTROSOMAL PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15431">
        <property type="entry name" value="FGFR1 ONCOGENE PARTNER/LISH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09398">
        <property type="entry name" value="FOP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FGFR1 oncogene partner (FOP) N-terminal dimerisation" evidence="14">
        <location>
            <begin position="48"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="242"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="321"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="461"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="268"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="321"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="380"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="409"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="461"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF09398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_047006850.1"/>
        </source>
    </evidence>
    <sequence length="541" mass="59547" checksum="64FB4EF55D87B51B" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979ENN6</accession>
    <name>A0A979ENN6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Osteoclast-stimulating factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">myo1ea</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Induces bone resorption, acting probably through a signaling cascade which results in the secretion of factor(s) enhancing osteoclast formation and activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 14">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047007603.1">
        <property type="nucleotide sequence ID" value="XM_047151647.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979ENN6"/>
    <dbReference type="GeneID" id="108259244"/>
    <dbReference type="KEGG" id="ipu:108259244"/>
    <dbReference type="CTD" id="555285"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003094">
        <property type="term" value="P:glomerular filtration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032835">
        <property type="term" value="P:glomerulus development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01378">
        <property type="entry name" value="MYSc_Myo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11827">
        <property type="entry name" value="SH3_MyoIe_If_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000004">
        <property type="entry name" value="Myosin IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000007">
        <property type="entry name" value="Myosin IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000101">
        <property type="entry name" value="Slow myosin heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000072">
        <property type="entry name" value="Unconventional Myosin IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000010">
        <property type="entry name" value="Unconventional myosin-Ie"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035507">
        <property type="entry name" value="Ie/If_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036072">
        <property type="entry name" value="MYSc_Myo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF341">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="9 14" id="KW-0505">Motor protein</keyword>
    <keyword evidence="8 14" id="KW-0518">Myosin</keyword>
    <keyword evidence="4 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 13" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Myosin motor" evidence="17">
        <location>
            <begin position="19"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="domain" description="TH1" evidence="18">
        <location>
            <begin position="729"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="1056"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="14">
        <location>
            <begin position="568"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="996"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="112"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_047007603.1"/>
        </source>
    </evidence>
    <sequence length="1113" mass="127159" checksum="C08B39E10C23F320" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EVB9</accession>
    <name>A0A979EVB9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">LOW QUALITY PROTEIN: crooked neck-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC108265189</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the crooked-neck family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047011742.1">
        <property type="nucleotide sequence ID" value="XM_047155786.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108265189"/>
    <dbReference type="KEGG" id="ipu:108265189"/>
    <dbReference type="OrthoDB" id="541719at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000117">
        <property type="entry name" value="Crooked neck pre-mRNA-splicing factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055430">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045075">
        <property type="entry name" value="Syf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246:SF3">
        <property type="entry name" value="CROOKED NECK-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23231">
        <property type="entry name" value="HAT_Syf1_CNRKL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1/CRNKL1-like C-terminal HAT-repeats" evidence="7">
        <location>
            <begin position="52"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_047011742.1"/>
        </source>
    </evidence>
    <sequence length="347" mass="41873" checksum="8D6E3D17782F75A2" modified="2023-02-22" version="1">MGARIFHLCEVDLHRQRSIAIANVLLIMEKRHWRRYIYSWIKYALFEELEVKDPERTRKVYQACLALIPDQKFTFAKIWLLYGQFEVRQKNLQNARRCLGTGIGKYPKNKLFKGYIELELQLREFDRCRKLYEKYLEFSPEKCTAWIKFAELETILGEIERVRAIFELAISQPYLDMPEVLWKSYIDFGIEQKEYENTCGLYKRLLQRTXHVKVWXSYAQFELSIEEGQLQRCRQVYEEANRDLCTCEEKEERLMLLEAWNEFEQEFGTLANKDRIRKLMPEKVKKRRKLTVEDGSDAEWEEYYDYIFPEDAANQPNFKLLAMAKMWKKQQQQQDQPQEERGTECAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EZQ5</accession>
    <name>A0A979EZQ5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein si:dkey-109l4.3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">si:dkey-109l4.3</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047013352.1">
        <property type="nucleotide sequence ID" value="XM_047157396.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EZQ5"/>
    <dbReference type="GeneID" id="124628441"/>
    <dbReference type="KEGG" id="ipu:124628441"/>
    <dbReference type="OrthoDB" id="691673at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_047013352.1"/>
        </source>
    </evidence>
    <sequence length="164" mass="18676" checksum="E390531F37836375" modified="2023-02-22" version="1">MELCGLQSSSNGHTHVQEWHHETSAGFLPPKRKRSDLTNMTGDRCDSSSAFPAKETAKNHSRFVRIDGQRGVYYSESFEPNGSTLYLPVCGTVLANMHKYEQISFEQGCFCVGDETGGFRQRVQGQALHCWRYRKDVRKLFIALSYFFTNCDVFQEVLSSLECL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F248</accession>
    <name>A0A979F248_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">pak2b</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_047013989.1">
        <property type="nucleotide sequence ID" value="XM_047158033.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979F248"/>
    <dbReference type="GeneID" id="108271286"/>
    <dbReference type="CTD" id="325034"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06655">
        <property type="entry name" value="STKc_PAK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000011">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000069">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035064">
        <property type="entry name" value="STK_PAK2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF21">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="176"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="66"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="143"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_047013989.1"/>
        </source>
    </evidence>
    <sequence length="451" mass="49985" checksum="9EA55EA9C0F80406" modified="2023-02-22" version="1">MVTFTSPKRYLGDLTICKLGMPEQWARLLQTSNITKSEQKKNPQAVLDVLKFYDSTGNTGRQKYLSFSSEKDGFPSADHSPVKKSTDKPSPNTKNAEDDDDDDETPPPVVAPRPEHTKGVNTRSVIDPIPAPVSPDGDVASKAADKQRPKKGKMSDEEIMDKLRTIVSIGDPKKKYTRYEKIGQGASGTVFTAIDVATGQEVAIKQINLQKQPKKELIINEILVMKELKNPNIVNFLDSFLVGEELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQSPEKLSPIFRDFLARCLEMDVEKRGSSKDLLQHPFLKLAKPLSSLTPLILAAKDAMKNNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F3Z9</accession>
    <name>A0A979F3Z9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Hyaluronidase</fullName>
            <ecNumber evidence="17">3.2.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">hyal1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 17">
            <text>Random hydrolysis of (1-&gt;4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.</text>
            <dbReference type="EC" id="3.2.1.35"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13 17">Belongs to the glycosyl hydrolase 56 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.35" evidence="17"/>
    <dbReference type="RefSeq" id="XP_047014630.1">
        <property type="nucleotide sequence ID" value="XM_047158674.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979F3Z9"/>
    <dbReference type="GeneID" id="108271980"/>
    <dbReference type="CTD" id="3373"/>
    <dbReference type="OrthoDB" id="5796153at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004415">
        <property type="term" value="F:hyalurononglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030214">
        <property type="term" value="P:hyaluronan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000065">
        <property type="entry name" value="Hyaluronidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018155">
        <property type="entry name" value="Hyaluronidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11769">
        <property type="entry name" value="HYALURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11769:SF23">
        <property type="entry name" value="HYALURONIDASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01630">
        <property type="entry name" value="Glyco_hydro_56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038193">
        <property type="entry name" value="Hyaluronidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00846">
        <property type="entry name" value="GLHYDRLASE56"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 17" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0458">Lysosome</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="14">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="15">
        <location>
            <position position="412"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="103"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="267"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="420"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="425"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="487"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR038193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR038193-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR038193-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR038193-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU610713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_047014630.1"/>
        </source>
    </evidence>
    <sequence length="507" mass="57910" checksum="AD15CD11A737D912" modified="2023-02-22" version="1">MPGPKVSQQNIECHTPSASLPSSHSASWCHLLTRLQGPPQLLERRENKQKFSKIICSMGTTAHWTCRLIFLCTFLTCPIVSCPLKLLPESPFFTVWNAPTAKCAAQYGVDLDLSIFDIIHNRNQSFNGSNIIIFYSDKLGFYPHYTDKNESVYGGVPQNFSLHDHLSQAWADLQNMMPDKAFSGLAVVDWERWRPLWERNWNSKEVYQQGSRALVRAKHPDWKPAQIEAKAKEDFEAAAQAFMEQTIKLGRDERPGGLWGFYGFPCCYNYQYKKNETYTGECPTLDMKRNNKLSWLWNVSTALYPDIYLDLSLKGHDRDIVLYAQHRIMEGMRVREQVSPTQPVVIPYARIVYTYSMTFLSQEDLVHTIGESVALGAAGVVLWGDAIYSTNKSTCLAVKNYLDETLGQYLVNVTEAAVLCSKKLCSFNGRCVRRDPGSSAYLHLDPAVWTIIHRARLPDLNTNSPPYVIQMNKEEQYHFTKPFKCQCYPGWGGKRCEQQDPKPGQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F8H8</accession>
    <name>A0A979F8H8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16 17" type="primary">LOC108276327</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16 17" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16 17">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_047016363.1">
        <property type="nucleotide sequence ID" value="XM_047160407.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053542065.1">
        <property type="nucleotide sequence ID" value="XM_053686090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979F8H8"/>
    <dbReference type="GeneID" id="108276327"/>
    <dbReference type="KEGG" id="ipu:108276327"/>
    <dbReference type="OrthoDB" id="8918678at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051138">
        <property type="entry name" value="PIM_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024298">
        <property type="entry name" value="Sec16_Sec23-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22984:SF11">
        <property type="entry name" value="AURORA KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22984">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12931">
        <property type="entry name" value="TPR_Sec16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="307"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_047016363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_053542065.1"/>
        </source>
    </evidence>
    <sequence length="555" mass="63904" checksum="170E9744300F194F" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F989</accession>
    <name>A0A979F989_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein Aster-B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">gramd1ba</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_047016633.2">
        <property type="nucleotide sequence ID" value="XM_047160677.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979F989"/>
    <dbReference type="GeneID" id="108277131"/>
    <dbReference type="CTD" id="568698"/>
    <dbReference type="OrthoDB" id="2162691at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140268">
        <property type="term" value="C:endoplasmic reticulum-plasma membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:cholesterol binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:cholesterol transfer activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="PH-GRAM_GRAMDC"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GRAM domain containing 1B"/>
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    </dbReference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR051482">
        <property type="entry name" value="Cholesterol_transport"/>
    </dbReference>
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        <property type="entry name" value="GRAM"/>
    </dbReference>
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        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319:SF3">
        <property type="entry name" value="PROTEIN ASTER-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="765"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="7">
        <location>
            <begin position="514"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="401"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="685"/>
            <end position="721"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
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            <begin position="49"/>
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        <location>
            <begin position="112"/>
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        </location>
    </feature>
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            <begin position="142"/>
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    </feature>
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        <location>
            <begin position="690"/>
            <end position="715"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="PROSITE" id="PS51778"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_047016633.2"/>
        </source>
    </evidence>
    <sequence length="877" mass="100644" checksum="C845900BA53446E4" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979FFS0</accession>
    <name>A0A979FFS0_ICTPU</name>
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        <submittedName>
            <fullName evidence="5">GRAM domain-containing protein 2A isoform X1</fullName>
        </submittedName>
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    <gene>
        <name evidence="5" type="primary">LOC108280868</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46645:SF1">
        <property type="entry name" value="GRAM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46645">
        <property type="entry name" value="GRAM DOMAIN-CONTAINING PROTEIN 2B-RELATED"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GRAM"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="GRAM" evidence="3">
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            <begin position="114"/>
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        </location>
    </feature>
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        </source>
    </evidence>
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            <dbReference type="SMART" id="SM00568"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_047018569.1"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QTI0</accession>
    <name>A0A9F7QTI0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein FAM131A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">fam131aa</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM131 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053529794.1">
        <property type="nucleotide sequence ID" value="XM_053673819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QTI0"/>
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    <dbReference type="KEGG" id="ipu:108280319"/>
    <dbReference type="CTD" id="100136860"/>
    <dbReference type="OrthoDB" id="8903525at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026782">
        <property type="entry name" value="FAM131"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15736:SF4">
        <property type="entry name" value="PROTEIN FAM131A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15736">
        <property type="entry name" value="PROTEIN FAM131B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15010">
        <property type="entry name" value="FAM131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="274"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053529794.1"/>
        </source>
    </evidence>
    <sequence length="288" mass="31877" checksum="9263B761B94A8B10" modified="2023-06-28" version="1">MCAMLLTALPQFSLKIDEPVEMLPKSRRALSIQEIAALARSSLNGISQVVKDHVTKPTAMAQGRVAHLIEWKGWSKPLEPSSATLQSHFNSYSHLSEGEQEARFAAGVAEQFAIAEAKLRAWNSVDEEDVEEECSEEELAHSNELTITTQSSDAMLSNQESLTPCQSETNQNISEESSLVLTHSDGHRNTEEPRGTVEELQIPSEDLQLPSDEVQKFKRRSLWSRGDSCYHSASYSESGLSPDEGEQGVEVEDNVFQEVTCWYSMSDTSGAASFDDEVDVEDGETSRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QV30</accession>
    <name>A0A9F7QV30_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Type-1 angiotensin II receptor A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC108255426</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053530515.1">
        <property type="nucleotide sequence ID" value="XM_053674540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QV30"/>
    <dbReference type="GeneID" id="108255426"/>
    <dbReference type="KEGG" id="ipu:108255426"/>
    <dbReference type="OMA" id="VEQQHWT"/>
    <dbReference type="OrthoDB" id="5968937at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004974">
        <property type="term" value="F:leukotriene receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000109">
        <property type="entry name" value="Leukotriene B4 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF946">
        <property type="entry name" value="LEUKOTRIENE B4 RECEPTOR 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="220"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="36"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_053530515.1"/>
        </source>
    </evidence>
    <sequence length="296" mass="33426" checksum="7D018B72C72F5B09" modified="2023-06-28" version="1">MQQLNSSNSSLTSPVLTESLIASSVLAVCFILGVPGNIAVMVRLAGWLKGGSFTPRLMLSLAISDLLTLLFLPVWIWALLYGWVFGLFLCKLLSYLVYLSLYCSMLCVMLMSVQRYIQVLHPQKWNKLGSKGRKILLSGMWMLSGVLSSYSLIQRTLSLDKEGHLQCRLHYHNDVERVATLIWEIMIFVVSFPTLTYFYFQLYRGVHNSAFFSSSLMTKLVTRIVACFFIFWVPFQISNIVLIIAVLLENDNLLKSAEAGSNITGALTFINSCVNPFLYAFSARALRQHTNETENT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QW71</accession>
    <name>A0A9F7QW71_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">P2Y purinoceptor 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC108257205</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053531384.1">
        <property type="nucleotide sequence ID" value="XM_053675409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QW71"/>
    <dbReference type="GeneID" id="108257205"/>
    <dbReference type="KEGG" id="ipu:108257205"/>
    <dbReference type="OrthoDB" id="10261452at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045031">
        <property type="term" value="F:G protein-coupled ATP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030594">
        <property type="term" value="F:neurotransmitter receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231:SF46">
        <property type="entry name" value="P2Y PURINOCEPTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231">
        <property type="entry name" value="PURINOCEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="178"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="32"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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    <sequence length="319" mass="36677" checksum="3BA1EE0CAD933F58" modified="2023-06-28" version="1">MVGNWNRSCNSTFQKEVLPPIYGVEMCVALVGNILALWLLVTKERKNWHTGVVFSCNLVISDIFYALTLPHLIDYYSRGRQWIFHDAVCKIERFLFTCNLYVSIYFIMCISVNRYLAIVHPFFTRKHIHPKHAKIISVFVWIFVASISSPLLYFSGVYNKERCFLFANIDKKSSLKSTYRVFMGVIGCFVPFVVTFASYFGVLWVVFKNANITSLEKKKVALIVGLVCVLYTVSFVPYHILQIVNFELREEGKTNCYVHNGYQVSKVLACLNMCLHPILYMAVSDSIRAVCCRRSSNVSNIQITVGEHSSKNFELKSRD</sequence>
</entry>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Liu S."/>
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                <person name="Bao L."/>
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                <person name="Li Y."/>
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                <person name="Yuan Z."/>
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                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
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                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="18">Blood</tissue>
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        <subcellularLocation>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="5" id="KW-0678">Repressor</keyword>
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    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
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        <location>
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        </location>
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    </feature>
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        </location>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="857"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
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            <begin position="433"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="483"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="614"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="806"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="861"/>
            <end position="873"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00015909"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022491"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50112"/>
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        <source>
            <dbReference type="PROSITE" id="PS50888"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_053532719.1"/>
        </source>
    </evidence>
    <sequence length="895" mass="99938" checksum="AFCBE611D0A5EBB0" modified="2023-06-28" version="1"> </sequence>
</entry>
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        <submittedName>
            <fullName evidence="11">CCN family member 2</fullName>
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        <name evidence="11" type="primary">LOC108281055</name>
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    <organism evidence="10 11">
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            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
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                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
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                <person name="Duke M.V."/>
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                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
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                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
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    <reference evidence="11" key="2">
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            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
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        <text evidence="2">Belongs to the CCN family.</text>
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    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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            <dbReference type="RefSeq" id="XP_053529882.1"/>
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    <sequence length="350" mass="39160" checksum="07B7A4AA24958E56" modified="2023-06-28" version="1">MEFRTSHRRGETVRLQDEEENPRSCVCYLCPPLCPHTGPCQCPLSLPTCPLGVPLVSDGCRCCQICAGQEGELCSEKDMCDAQRGLECDYSASFPDGRGECVRRSTLGCEHLGVRYEEGQSFIPSCRQLCRCVGGGVTCVPLCTEDLNTPNCQHPRLVHVLGRCCRKWVCDGTENNIYIFAVMVAERAVRSWQDTLAPNRISWTNCIQHSTDWSVCSRSCGPGVSTRVSNRNLPCQPETHMRLCVVRPCHPEPTARAQPKPGVCQSSYRSPVLVHFEHQGCYSARSYRPLFCGMCSDGRCCSPDRTRTALVTFRCPRGRLAQHAVMMIKSCVCHYDCPHPNSSRRTRSWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2025-10-08" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QZG3</accession>
    <name>A0A9F7QZG3_ICTPU</name>
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        <submittedName>
            <fullName evidence="5 6 7">Pericentriolar material 1 protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6 7 8 9" type="primary">pcm1</name>
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    <organism evidence="4 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">SDA103</strain>
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    </reference>
    <reference evidence="5 6 7" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5 6 7">Blood</tissue>
        </source>
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        <property type="nucleotide sequence ID" value="XM_053677372.1"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:non-motile cilium assembly"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="PCM1_C"/>
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    <dbReference type="InterPro" id="IPR024138">
        <property type="entry name" value="Pericentriolar_Pcm1"/>
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        <property type="entry name" value="PERICENTRIOLAR MATERIAL 1 PROTEIN"/>
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        <property type="entry name" value="PERICENTRIOLAR MATERIAL 1-RELATED"/>
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        <property type="entry name" value="PCM1_C"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <begin position="1392"/>
            <end position="1987"/>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="35"/>
            <end position="94"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="406"/>
            <end position="448"/>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="490"/>
            <end position="534"/>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="668"/>
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    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="740"/>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="902"/>
            <end position="1014"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1175"/>
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            <begin position="1459"/>
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    </feature>
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        <location>
            <begin position="1718"/>
            <end position="1952"/>
        </location>
    </feature>
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            <begin position="321"/>
            <end position="361"/>
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    </feature>
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        <location>
            <begin position="778"/>
            <end position="815"/>
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    </feature>
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            <begin position="52"/>
            <end position="65"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="115"/>
            <end position="133"/>
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        <location>
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            <begin position="1245"/>
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        <location>
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        <location>
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        <location>
            <begin position="1802"/>
            <end position="1816"/>
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        <location>
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        <location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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        <source>
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        <source>
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            <taxon>Ictalurus</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
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                <person name="Liu H."/>
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                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
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                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        </location>
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    <feature type="chain" id="PRO_5039927843" evidence="1">
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            <begin position="25"/>
            <end position="528"/>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053530171.1"/>
        </source>
    </evidence>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
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                <person name="Zimin A."/>
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                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
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                <person name="Wang X."/>
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                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
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                <person name="Lu J."/>
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                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
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                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
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    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <source>
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        <source>
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        <source>
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    </evidence>
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</entry>
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    <accession>A0A9F7R876</accession>
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    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053538030.1">
        <property type="nucleotide sequence ID" value="XM_053682055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R876"/>
    <dbReference type="GeneID" id="108261667"/>
    <dbReference type="KEGG" id="ipu:108261667"/>
    <dbReference type="OrthoDB" id="2789670at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000004">
        <property type="entry name" value="cytochrome P450 2D15 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF327">
        <property type="entry name" value="CYTOCHROME P450 2F2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="7 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039886854" evidence="12">
        <location>
            <begin position="22"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="518"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_053538030.1"/>
        </source>
    </evidence>
    <sequence length="578" mass="66120" checksum="933F1BC599342B47" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RFZ4</accession>
    <name>A0A9F7RFZ4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Gastrula zinc finger protein XlCGF7.1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC108279949</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053544325.1">
        <property type="nucleotide sequence ID" value="XM_053688350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RFZ4"/>
    <dbReference type="GeneID" id="108279949"/>
    <dbReference type="KEGG" id="ipu:108279949"/>
    <dbReference type="OrthoDB" id="9411774at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001639">
        <property type="entry name" value="Si:dkey-7i4.21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000966">
        <property type="entry name" value="ZFP90 zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000097">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002005">
        <property type="entry name" value="Zinc finger protein 200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002254">
        <property type="entry name" value="Zinc finger protein 540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000852">
        <property type="entry name" value="zinc finger protein 629 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24409">
        <property type="entry name" value="ZINC FINGER PROTEIN 142"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24409:SF331">
        <property type="entry name" value="ZINC FINGER PROTEIN 322A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="23"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="107"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="163"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="219"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="247"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="275"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_053544325.1"/>
        </source>
    </evidence>
    <sequence length="333" mass="39234" checksum="92BDD83B07B64385" modified="2023-06-28" version="1">MKSRRHSLRKSPQTPDATGSEMYHCSDCGESFTESHHFKDHKWIHTVEKPFYCGQCGKSFTHLYDLQRHERIHTGQKPYHCGQCEKSFTFQSNLQQHELIHTGEKPYYCSQCGKSFARKSNLQRHERIHTEEKPYYCSQCGKSFTCNNNLQQHKYIHTGEKPYYCSQCGKSFTFQSNLQRHERIHTGEKPYHCTQCGKSFSQLYTLQQHKYIHTGEKLYHCGQCGKSFTCQFYLQRHERIHTGEKLYHCGQCGKSFTCQRYLQQHERIHTGEKLYYCSQCGKSFRDQSRLHSHQQSHTGQKPYLCGHCGRSYTHSSSLRRHKCSNIKPSDLAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RIJ1</accession>
    <name>A0A9F7RIJ1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Integrin alpha-M</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC108273638</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053540892.1">
        <property type="nucleotide sequence ID" value="XM_053684917.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RIJ1"/>
    <dbReference type="GeneID" id="108273638"/>
    <dbReference type="KEGG" id="ipu:108273638"/>
    <dbReference type="OrthoDB" id="5317514at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000012">
        <property type="entry name" value="Integrin, alpha 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048633">
        <property type="entry name" value="ITGAX-like_Ig_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF118">
        <property type="entry name" value="INTEGRIN ALPHA-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08441">
        <property type="entry name" value="Integrin_A_Ig_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21520">
        <property type="entry name" value="ITGAX-like_Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 16" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 16 19" id="KW-0401">Integrin</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039961710" evidence="16">
        <location>
            <begin position="26"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1087"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="17">
        <location>
            <begin position="156"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="441"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="503"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_053540892.1"/>
        </source>
    </evidence>
    <sequence length="1136" mass="125922" checksum="3C8A9E59A5727F6E" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RJJ5</accession>
    <name>A0A9F7RJJ5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">T-box transcription factor 16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tbx16</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053536547.1">
        <property type="nucleotide sequence ID" value="XM_053680572.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RJJ5"/>
    <dbReference type="GeneID" id="108265264"/>
    <dbReference type="KEGG" id="ipu:108265264"/>
    <dbReference type="CTD" id="30264"/>
    <dbReference type="OrthoDB" id="7442607at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060429">
        <property type="term" value="P:epithelium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003007">
        <property type="term" value="P:heart morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007389">
        <property type="term" value="P:pattern specification process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20197">
        <property type="entry name" value="T-box_VegT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.820:FF:000007">
        <property type="entry name" value="T-box transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.820">
        <property type="entry name" value="Transcription factor, T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046360">
        <property type="entry name" value="T-box_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036960">
        <property type="entry name" value="T-box_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001699">
        <property type="entry name" value="TF_T-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018186">
        <property type="entry name" value="TF_T-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267:SF204">
        <property type="entry name" value="SPADETAIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267">
        <property type="entry name" value="T-BOX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00907">
        <property type="entry name" value="T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00937">
        <property type="entry name" value="TBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00425">
        <property type="entry name" value="TBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01283">
        <property type="entry name" value="TBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01264">
        <property type="entry name" value="TBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50252">
        <property type="entry name" value="TBOX_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="T-box" evidence="8">
        <location>
            <begin position="35"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="208"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="241"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="282"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="296"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="327"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053536547.1"/>
        </source>
    </evidence>
    <sequence length="464" mass="52013" checksum="8929E67866CB008F" modified="2023-06-28" version="1">MQTIRDLKHNFSIPPPSAMAGATDSFHQGNIRITLEDPELWKSFHEIGTEMIITKPGRRMFPHCKINISGLVPYAKYILLVDIVPEDGFRYKWNKDKWEVAGKAEPQPPYRTYLHPDSPAPGSHWMKQSVSFLKLKLTNNALDQHGHIILHSMHRYHPRFHIVQADDLYSVRWSVFQTFTFPETSFTAVTAYQNTKITKLKIDNNPFAKGFRDEGMNSKRRANRGPADTERLAKRLNSLVRDSDQDSSPDLRRSSYEGLAEAQAGCKEGVKEEPNSPWGGASDRENSHSLGRDSPLGSNTRDIYSSEQLVPGHGTYQPYRFPDYNKSTSPTSSSMSSSAGRSSFESRVPDIATVPEQDSKVTDLVVPQCPPPATAGVQDYAGVLNVAVAQAKPGMLGHHALYAPYSTDQTLSQWSGTSSAQYPSHPHHHHHLAADYGTQAVHHGYHHANMADWSQYPLFSYSCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RJP4</accession>
    <name>A0A9F7RJP4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7 8">Guanine nucleotide exchange factor for Rab-3A isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">rab3il1</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7 8" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7 8">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053541491.1">
        <property type="nucleotide sequence ID" value="XM_053685516.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053541492.1">
        <property type="nucleotide sequence ID" value="XM_053685517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RJP4"/>
    <dbReference type="GeneID" id="108274990"/>
    <dbReference type="KEGG" id="ipu:108274990"/>
    <dbReference type="CTD" id="5866"/>
    <dbReference type="OrthoDB" id="5560525at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070319">
        <property type="term" value="C:Golgi to plasma membrane transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040351">
        <property type="entry name" value="RAB3IL/RAB3IP/Sec2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009449">
        <property type="entry name" value="Sec2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14430:SF5">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR FOR RAB-3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14430">
        <property type="entry name" value="RABIN3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25555">
        <property type="entry name" value="RAB3A-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06428">
        <property type="entry name" value="Sec2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144284">
        <property type="entry name" value="Sec2 N-terminal region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GDP/GTP exchange factor Sec2 N-terminal" evidence="5">
        <location>
            <begin position="93"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="210"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="122"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053541491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053541492.1"/>
        </source>
    </evidence>
    <sequence length="433" mass="48332" checksum="2675A64B8D63D468" modified="2023-06-28" version="1">MDAFEGIHTVCISSSPPAVSTPSPGYELLVTGSKGIALYSSHAFFGKVDSSGHQQSKHSRAKKEECPVGKLIDLEAELEPGTESGEGRGDTMLQDEAEAQRREAYSVSRLRSSSIEIREKGTEFLREQLDEAQKELKLKGEECERLSHLRNQLEQELEELTASLFEEAHKMVREANVKQATAEKQLKEAQGKIDVLQAEVTALKTLVLTSTPSSPNRQLHPQLLSPGSRGHTRHKSATLTLPQLQSESPSENAHISREEKELDSMLFAEFLSWREKPSLDRSSAFLSRIYKEDIGPCLSFTCSELSHSVQNAVENNFLTIEPVALTAISTDKASTVEFGGPNGCRAPVETKCALSGMSHLCRHRIKLGDKENYYYISPSSRARITAVCNFFTYIRYIQQGLVRHDAEQMFCEVMRLRREMSVAKLGFFFADVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TCU7</accession>
    <name>A0A9F7TCU7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Chemokine-like protein TAFA-1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC108280984</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TAFA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053530133.1">
        <property type="nucleotide sequence ID" value="XM_053674158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TCU7"/>
    <dbReference type="GeneID" id="108280984"/>
    <dbReference type="OrthoDB" id="9924724at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048018">
        <property type="term" value="F:receptor ligand activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014016">
        <property type="term" value="P:neuroblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902692">
        <property type="term" value="P:regulation of neuroblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020350">
        <property type="entry name" value="Chemokine-like_TAFA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051743">
        <property type="entry name" value="TAFA_chemokine-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31770">
        <property type="entry name" value="CHEMOKINE-LIKE PROTEIN TAFA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31770:SF2">
        <property type="entry name" value="CHEMOKINE-LIKE PROTEIN TAFA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12020">
        <property type="entry name" value="TAFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039955063" evidence="3">
        <location>
            <begin position="20"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053530133.1"/>
        </source>
    </evidence>
    <sequence length="138" mass="15423" checksum="A2361D141B705BBC" modified="2023-06-28" version="1">MSWFLCLWIAVSCLVLCQATLYETIQQHHVPRPGRNAIQILEGGTCEVIAAHRCCNKNRIEERSQTVKCSCLPGKVAGTTRNKPSCVDASIVIGKWWCEMEPCLEGEECKTLPDNSGWMCSSGNKIKTTRNTQPYSPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TDP1</accession>
    <name>A0A9F7TDP1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein PAT1 homolog 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">PAT1-like protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Protein PAT1 homolog b</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">patl1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">PML body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PAT1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053533519.1">
        <property type="nucleotide sequence ID" value="XM_053677544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TDP1"/>
    <dbReference type="GeneID" id="108260591"/>
    <dbReference type="KEGG" id="ipu:108260591"/>
    <dbReference type="CTD" id="219988"/>
    <dbReference type="OrthoDB" id="74835at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039900">
        <property type="entry name" value="Pat1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019167">
        <property type="entry name" value="PAT1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21551:SF2">
        <property type="entry name" value="PROTEIN PAT1 HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21551">
        <property type="entry name" value="TOPOISOMERASE II-ASSOCIATED PROTEIN PAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09770">
        <property type="entry name" value="PAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="mRNA decay factor PAT1" evidence="13">
        <location>
            <begin position="490"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="55"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="220"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="220"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF09770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_053533519.1"/>
        </source>
    </evidence>
    <sequence length="771" mass="86348" checksum="939520CC1CD526A4" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TEV0</accession>
    <name>A0A9F7TEV0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">C-type lectin domain family 19 member A isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">clec19a</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053532653.1">
        <property type="nucleotide sequence ID" value="XM_053676678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TEV0"/>
    <dbReference type="GeneID" id="108280864"/>
    <dbReference type="KEGG" id="ipu:108280864"/>
    <dbReference type="CTD" id="728276"/>
    <dbReference type="OrthoDB" id="418245at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="CDD" id="cd03589">
        <property type="entry name" value="CLECT_CEL-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033988">
        <property type="entry name" value="CEL1-like_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01504">
        <property type="entry name" value="PNCREATITSAP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039918183" evidence="1">
        <location>
            <begin position="20"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="51"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053532653.1"/>
        </source>
    </evidence>
    <sequence length="191" mass="21880" checksum="968F9E42AC46801B" modified="2023-06-28" version="1">MLWCGLCLALWLSSAPLWALPSTSMKITQALPLQLPDSSQPAVCPLFWTEFDGYCYRFFPLNRTWAEADLYCAEFSNGHRSGKLASVHSWEENVFVYDLVNSRVPGIPTDIWIGLHDRRQEGTMEWTDGSPYEYSYWDGNQPDDGIHRISEEEDCVEIWYRQNSGPGMTTAVTKLFLLCVKSPCWSTNVLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TEY7</accession>
    <name>A0A9F7TEY7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Vasoactive intestinal polypeptide receptor 1b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">vipr1b</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053530702.1">
        <property type="nucleotide sequence ID" value="XM_053674727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TEY7"/>
    <dbReference type="GeneID" id="108256372"/>
    <dbReference type="KEGG" id="ipu:108256372"/>
    <dbReference type="CTD" id="503868"/>
    <dbReference type="OMA" id="WDIIEST"/>
    <dbReference type="OrthoDB" id="5967113at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017046">
        <property type="term" value="F:peptide hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004999">
        <property type="term" value="F:vasoactive intestinal polypeptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.10:FF:000016">
        <property type="entry name" value="Vasoactive intestinal peptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000032">
        <property type="entry name" value="Vasoactive intestinal polypeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050332">
        <property type="entry name" value="GPCR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001771">
        <property type="entry name" value="GPCR_2_VIP_rcpt_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620">
        <property type="entry name" value="PDF RECEPTOR-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620:SF24">
        <property type="entry name" value="VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02793">
        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01154">
        <property type="entry name" value="VIP1RECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111418">
        <property type="entry name" value="Hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00649">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039926956" evidence="13">
        <location>
            <begin position="23"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="289"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="368"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="14">
        <location>
            <begin position="38"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="15">
        <location>
            <begin position="132"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_053530702.1"/>
        </source>
    </evidence>
    <sequence length="454" mass="51950" checksum="1AD693A9AC659DD2" modified="2023-06-28" version="1">MDALRFVMFVSSLSFFLSTSSAIQMCDVVKEIKLEQEQCENRTAANITSGCKGMWDRIACWPTAKVGELVVIPCPNYFRYLSEQHNGGNLSKTCTADGWTEVLPIMISENCGYNPNGTVDDISSKHNFYQAVKIGYTIGHSVSLISLTTAIIILCIFRKLHCTRNYIHMHLFVAFILKAISLFIKDDVVLYDVVQESDDCFTSSSLVGCKAAIIFFQYCIMASFFWLLVEGLYLHALLAVSFFSERKYFWWYILIGWGGPMIFITAWSFAKAYFYDVGCWELIENSDPFWWIIKTPILASILMNFILFICIIRILRQKINCPDIGRNESNQYSRLAKSTLLLIPLFGINFIIFAFIPEHVERQLRLVFDLILGSFQGFVVAVLYCFLNGEVQAEIKRKWRRWHLERFLGSGTKYQHPSIGSNGNNFSTQISMLTRCSPKTQRASTCHDDSSITI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TH40</accession>
    <name>A0A9F7TH40_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">THO complex subunit 6 homolog isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">thoc6</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat THOC6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053532729.1">
        <property type="nucleotide sequence ID" value="XM_053676754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TH40"/>
    <dbReference type="GeneID" id="108280484"/>
    <dbReference type="KEGG" id="ipu:108280484"/>
    <dbReference type="CTD" id="79228"/>
    <dbReference type="OrthoDB" id="273067at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000347">
        <property type="term" value="C:THO complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000346">
        <property type="term" value="C:transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042626">
        <property type="entry name" value="THOC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44411">
        <property type="entry name" value="THO COMPLEX SUBUNIT 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44411:SF1">
        <property type="entry name" value="THO COMPLEX SUBUNIT 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="212"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053532729.1"/>
        </source>
    </evidence>
    <sequence length="387" mass="42717" checksum="81BFB05F92DBC44B" modified="2023-06-28" version="1">MIHSCIRVPWSSVSSFTPGLNGIFPVNPGFRFTLISDGLESSSEALIRSEFILKLFLCVLIRNFFARFAFRFLKIHFVPGLMVCCDWLDFIVWCLLFLCSLSAALSPDATEASQKPVLTFTAHEGPVFSLISTDTHLLSSGNGEISAWSWAELGKKNTKAVWTKRPDYKTGLEIPEINAMIINSRENSLVVGGGDNNIHIMDLETGTFKSVLQGHTDYIHCLSLRERDGEILSGAEDGAVRMWDGRTGQSVHCVEVYKYEECARPQYGKWISCVTTDSDWMLCGGGPALSLWHLRSMSPTSIFPLSGCQREAVFHQDLIFAVGEGPHVSHCLLGGSVKAQIPCTPPSLNTLALNLGTTEHRVMTVAGSSNQIDVFTNFSYRAFSLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TJ84</accession>
    <name>A0A9F7TJ84_ICTPU</name>
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        <submittedName>
            <fullName evidence="3">Histone-lysine N-methyltransferase NSD3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108267344</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053537258.1">
        <property type="nucleotide sequence ID" value="XM_053681283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TJ84"/>
    <dbReference type="GeneID" id="108267344"/>
    <dbReference type="OrthoDB" id="16287at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005700">
        <property type="term" value="C:polytene chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042799">
        <property type="term" value="F:histone H4K20 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043516">
        <property type="term" value="P:regulation of DNA damage response, signal transduction by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051760">
        <property type="entry name" value="KMT5A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46167">
        <property type="entry name" value="N-LYSINE METHYLTRANSFERASE KMT5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46167:SF1">
        <property type="entry name" value="N-LYSINE METHYLTRANSFERASE KMT5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="1">
        <location>
            <begin position="22"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053537258.1"/>
        </source>
    </evidence>
    <sequence length="294" mass="33183" checksum="60C837BFA5F6FBE1" modified="2023-06-28" version="1">MSQRKSPINNAIFHITTSRDKAGLDVKYIDAFKGTGVFTMTHFTKGEFINSLESNRRHRIYHNNLKGFMFDFIKFWTIDAARDDGTLGRLVNDDHINPNCKMKRIIVKGKPHLCLFALRDITPGEEVTYNYGDADCPWRSKCTKCGMDANTNRLESGATSGDVTQCTQHSQHKSASHIEVTESVIEVDSNRLESDGASGDDHHCTQHSQDESTFCNLVTESGIEVDSNRLESDGASRDDHHCTQHSQHESSFCNLVWNCLFNIFYNIVNVKSPQCTLYYTSVFKDTASPHCLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2007-07-10" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>A5X385</accession>
    <name>A5X385_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16 18">Toll-like receptor 20a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">TLR-20a</name>
        <name evidence="18" type="synonym">tlr-20a</name>
        <name evidence="18" type="synonym">TLR20-1</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 18" key="1">
        <citation type="journal article" date="2007" name="Fish Shellfish Immunol." volume="23" first="1218" last="1230">
            <title>Divergent Toll-like receptors in catfish (Ictalurus punctatus): TLR5S, TLR20, TLR21.</title>
            <authorList>
                <person name="Baoprasertkul P."/>
                <person name="Xu P."/>
                <person name="Peatman E."/>
                <person name="Kucuktas H."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="17981052"/>
            <dbReference type="DOI" id="10.1016/j.fsi.2007.06.002"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="journal article" date="2016" name="Biol. Reprod." volume="95" first="19" last="19">
            <title>Transcriptome Display During Testicular Differentiation of Channel Catfish (Ictalurus punctatus) as Revealed by RNA-Seq Analysis.</title>
            <authorList>
                <person name="Zeng Q."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Zhang Y."/>
                <person name="Yuan Z."/>
                <person name="Jiang C."/>
                <person name="Chen A."/>
                <person name="Fu Q."/>
                <person name="Su B."/>
                <person name="Dunham R."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="27307075"/>
            <dbReference type="DOI" id="10.1095/biolreprod.116.138818"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="17" key="4">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="5">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Toll-like receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="DQ529274">
        <property type="protein sequence ID" value="ABF74620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DQ529275">
        <property type="protein sequence ID" value="ABF74621.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187159.1">
        <property type="nucleotide sequence ID" value="NM_001200230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A5X385"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020983"/>
    <dbReference type="GeneID" id="100304982"/>
    <dbReference type="KEGG" id="ipu:100304982"/>
    <dbReference type="CTD" id="100304982"/>
    <dbReference type="OrthoDB" id="1421090at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002224">
        <property type="term" value="P:toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000001">
        <property type="entry name" value="Toll-like receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365:SF522">
        <property type="entry name" value="LOW QUALITY PROTEIN: TOLL-LIKE RECEPTOR 13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365">
        <property type="entry name" value="TOLL-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="13" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="15">
        <location>
            <begin position="202"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABF74620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="NP_001187159.1"/>
        </source>
    </evidence>
    <sequence length="351" mass="41235" checksum="4F15592678F1167D" modified="2007-07-10" version="1">MDLTDLNQLVNLKSLILFNVDLSHQSGLDMIFHNLSNLEYLYVSLWSVTFFNKDLTRDLQSLKVLYLHANDVFSVMENFVEPLKNLQYLIMDKALLYCICDNAWITNWAKYQQSVQVYFPGSSLESLPCKTSHGKQFLHKYAQDHCLTDIDFLLFASTSLGLVFFMLVVLLHQLAGDYLLAFFHIARAWVEEAMRANRKGHYHFDVFVSYCGKDERWVVDELLPNLEKRGPPFLRLCLHSRDFELGKDIVENITDSLYRSRHTLCLVSRNYLRSKWCSLEMRLATYRLLAEHRDVLVLVFLEKVPHQLLNVHHRLSRLVKTQTYIDWPQDPALHNAFWDRLWTKLAPETAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3TE05</accession>
    <name>E3TE05_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4 6">Vacuolar protein sorting-associated protein 26B-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">V26BL</name>
        <name evidence="6" type="synonym">v26bl</name>
        <name evidence="6" type="synonym">vps26bl</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="journal article" date="2016" name="Biol. Reprod." volume="95" first="19" last="19">
            <title>Transcriptome Display During Testicular Differentiation of Channel Catfish (Ictalurus punctatus) as Revealed by RNA-Seq Analysis.</title>
            <authorList>
                <person name="Zeng Q."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Zhang Y."/>
                <person name="Yuan Z."/>
                <person name="Jiang C."/>
                <person name="Chen A."/>
                <person name="Fu Q."/>
                <person name="Su B."/>
                <person name="Dunham R."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="27307075"/>
            <dbReference type="DOI" id="10.1095/biolreprod.116.138818"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="5">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS26 family.</text>
    </comment>
    <dbReference type="EMBL" id="GU588585">
        <property type="protein sequence ID" value="ADO28541.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001188246.1">
        <property type="nucleotide sequence ID" value="NM_001201317.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TE05"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000010482"/>
    <dbReference type="GeneID" id="100529047"/>
    <dbReference type="KEGG" id="ipu:100529047"/>
    <dbReference type="CTD" id="436688"/>
    <dbReference type="GeneTree" id="ENSGT00950000183064"/>
    <dbReference type="OMA" id="CNVLITF"/>
    <dbReference type="OrthoDB" id="3821113at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000001">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000002">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028934">
        <property type="entry name" value="Vps26-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12233">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING 26 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03643">
        <property type="entry name" value="Vps26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADO28541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="NP_001188246.1"/>
        </source>
    </evidence>
    <sequence length="332" mass="38638" checksum="2E2BC82D2C5DA17E" modified="2011-01-11" version="1">MSFFGFGQSAEIDIVLNDAETRKKVEHKTEDGKKDKYFLFYDGETVSGKVNITLKNPGKRLEHQGIKIEFIGQIELYYDRGNHHEFVSLVKDLARPGEMSQSQTFDFEFTHVEKPYESYTGQNVKLRYFLRATVSRRLNDISKEMDIVVHTLSTYPELNSSIKMEVGIEDCLHIEFEYNKSKYHLKDVIVGKIYFLLVRIKIKHMEIDIIKRETTGTGPNVYHENDTIAKYEIMDGAPVRGESIPIRLFLAGYEMTPTMRDINKKFSVRYYLNLVLIDEEERRYFKQQEITLWRKGDIVRKSMSHQAAIASQRFEGSASAERAIAQAKEDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3TE42</accession>
    <name>E3TE42_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Diamine acetyltransferase 1</fullName>
            <ecNumber evidence="5">2.3.1.57</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Polyamine N-acetyltransferase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Putrescine acetyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Spermidine/spermine N(1)-acetyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">SAT1</name>
        <name evidence="19" type="synonym">sat1</name>
        <name evidence="19" type="synonym">sat1a.2</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 19" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2016" name="Biol. Reprod." volume="95" first="19" last="19">
            <title>Transcriptome Display During Testicular Differentiation of Channel Catfish (Ictalurus punctatus) as Revealed by RNA-Seq Analysis.</title>
            <authorList>
                <person name="Zeng Q."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Zhang Y."/>
                <person name="Yuan Z."/>
                <person name="Jiang C."/>
                <person name="Chen A."/>
                <person name="Fu Q."/>
                <person name="Su B."/>
                <person name="Dunham R."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="27307075"/>
            <dbReference type="DOI" id="10.1095/biolreprod.116.138818"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>an alkane-alpha,omega-diamine + acetyl-CoA = an N-acetylalkane-alpha,omega-diamine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11116"/>
            <dbReference type="Rhea" id="RHEA-COMP:9766"/>
            <dbReference type="Rhea" id="RHEA-COMP:9767"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:70977"/>
            <dbReference type="ChEBI" id="CHEBI:70988"/>
            <dbReference type="EC" id="2.3.1.57"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:11117"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>spermidine + acetyl-CoA = N(1)-acetylspermidine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28150"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:58324"/>
            <dbReference type="EC" id="2.3.1.57"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:28151"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>spermine + acetyl-CoA = N(1)-acetylspermine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33099"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:45725"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58101"/>
            <dbReference type="EC" id="2.3.1.57"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:33100"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amine and polyamine degradation; putrescine degradation; N-acetylputrescine from putrescine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.57" evidence="5"/>
    <dbReference type="EMBL" id="GU588622">
        <property type="protein sequence ID" value="ADO28578.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187387.1">
        <property type="nucleotide sequence ID" value="NM_001200458.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TE42"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000031300"/>
    <dbReference type="GeneID" id="100528188"/>
    <dbReference type="KEGG" id="ipu:100528188"/>
    <dbReference type="CTD" id="431716"/>
    <dbReference type="GeneTree" id="ENSGT00950000183121"/>
    <dbReference type="OMA" id="NIQWQTP"/>
    <dbReference type="OrthoDB" id="7305308at2759"/>
    <dbReference type="UniPathway" id="UPA00188">
        <property type="reaction ID" value="UER00363"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004145">
        <property type="term" value="F:diamine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019809">
        <property type="term" value="F:spermidine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009447">
        <property type="term" value="P:putrescine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032918">
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        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
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                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
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                <person name="Ke C."/>
                <person name="Liu Z."/>
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        <citation type="journal article" date="2016" name="Biol. Reprod." volume="95" first="19" last="19">
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Zhang Y."/>
                <person name="Yuan Z."/>
                <person name="Jiang C."/>
                <person name="Chen A."/>
                <person name="Fu Q."/>
                <person name="Su B."/>
                <person name="Dunham R."/>
                <person name="Liu Z."/>
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            <dbReference type="DOI" id="10.1095/biolreprod.116.138818"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
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                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
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                <person name="Flanagan M.E."/>
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        <source>
            <strain evidence="3">SDA103</strain>
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    <reference evidence="4" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
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    <reference evidence="6 8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
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        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
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                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="8" key="3">
        <citation type="journal article" date="2016" name="Biol. Reprod." volume="95" first="19" last="19">
            <title>Transcriptome Display During Testicular Differentiation of Channel Catfish (Ictalurus punctatus) as Revealed by RNA-Seq Analysis.</title>
            <authorList>
                <person name="Zeng Q."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Zhang Y."/>
                <person name="Yuan Z."/>
                <person name="Jiang C."/>
                <person name="Chen A."/>
                <person name="Fu Q."/>
                <person name="Su B."/>
                <person name="Dunham R."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="27307075"/>
            <dbReference type="DOI" id="10.1095/biolreprod.116.138818"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
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    <reference evidence="8" key="5">
        <citation type="submission" date="2025-04" db="UniProtKB">
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    </dbReference>
    <dbReference type="GO" id="GO:0004303">
        <property type="term" value="F:estradiol 17-beta-dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006694">
        <property type="term" value="P:steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05356">
        <property type="entry name" value="17beta-HSD1_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000137">
        <property type="entry name" value="Hydroxysteroid (17-beta) dehydrogenase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051019">
        <property type="entry name" value="VLCFA-Steroid_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43899">
        <property type="entry name" value="RH59310P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43899:SF18">
        <property type="entry name" value="VERY-LONG-CHAIN 3-OXOACYL-COA REDUCTASE-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000126">
        <property type="entry name" value="11-beta-HSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADO28657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="NP_001187450.1"/>
        </source>
    </evidence>
    <sequence length="317" mass="34943" checksum="2E93CC6BB48A7D4A" modified="2011-01-11" version="1">MEAADILKQVKVLLFWVGAVTVAWVSLCTLYRLFNGLRVWVLGNGNLIKASKLGKWAVITGATDGIGKAYAEELARRGFAIVLISRTQEKPDDVSKAIESKYNVETKTISADFGSVDIYSKIESGLAGLEIGILVNNVGVSYSYPEFFLNIPNLDNFVNNMININVTSVCQMTRLVLPRMVDRSKGVILNIASASGMYPVPLLTLYSSSKAFVNFFSRGLDAEYRSKGIIIQSVLPFYVTTKLSKIKRASLDIPTPETYVASQLSTVSLQSQTNGYLPHAIMGWVTTSLLPAQLLTKYVMNMGLSQRARYLKKQKQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3TFC4</accession>
    <name>E3TFC4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Integral membrane protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ITM2B</name>
        <name evidence="18" type="synonym">itm2b</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 18" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Membrane</location>
            <topology evidence="14">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the ITM2 family.</text>
    </comment>
    <dbReference type="EMBL" id="GU589058">
        <property type="protein sequence ID" value="ADO29010.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187703.1">
        <property type="nucleotide sequence ID" value="NM_001200774.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TFC4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007222"/>
    <dbReference type="GeneID" id="100528504"/>
    <dbReference type="KEGG" id="ipu:100528504"/>
    <dbReference type="CTD" id="9445"/>
    <dbReference type="OrthoDB" id="9982095at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001540">
        <property type="term" value="F:amyloid-beta binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042985">
        <property type="term" value="P:negative regulation of amyloid precursor protein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007084">
        <property type="entry name" value="BRICHOS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040145">
        <property type="entry name" value="ITM2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10962:SF4">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10962">
        <property type="entry name" value="INTEGRAL TRANSMEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04089">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01039">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50869">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="BRICHOS" evidence="15">
        <location>
            <begin position="129"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ADO29010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="NP_001187703.1"/>
        </source>
    </evidence>
    <sequence length="259" mass="29920" checksum="9DD54B5E221801D8" modified="2011-01-11" version="1">MVKVTFNSALAQKEVKKDSETLIPDDKDPEAAQVVGHPSRVWCWCLYLGLALMLSGVVVGGAYLYKYYVLEEGQVFVCGTKYREENYRIQEENDDLLQECDLSLRRIDEIVRVLQDEEVELINVQVPNFSDSDPAIIVHDFKRRLTAYLDLTLDKCYVIPLNTSVVMPPHDLMDLLAKLKAGTYLPQTYLVHEQMIVTEKLDDIEPLGTYIYKVCQNKDTYRLQRRDTILGLQKCEALNCHKIRHFENHFAVETLICEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3TG86</accession>
    <name>E3TG86_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Phosphatidylethanolamine N-methyltransferase</fullName>
            <shortName evidence="27">PEAMT</shortName>
            <shortName evidence="27">PEMT</shortName>
            <ecNumber evidence="27">2.1.1.17</ecNumber>
            <ecNumber evidence="27">2.1.1.71</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="27">Phospholipid methyltransferase</fullName>
            <shortName evidence="27">PLMT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27 29" type="primary">PEMT</name>
        <name evidence="31" type="synonym">pemt</name>
    </gene>
    <organism evidence="29">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="29 31" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30">SDA103</strain>
        </source>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="27">Catalyzes the three sequential steps of the methylation pathway for the biosynthesis of phosphatidylcholine, a critical and essential component for membrane structure. Uses S-adenosylmethionine (S-adenosyl-L-methionine, SAM or AdoMet) as the methyl group donor for the methylation of phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine, PE) to phosphatidylmonomethylethanolamine (1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine, PMME), PMME to phosphatidyldimethylethanolamine (1,2-diacyl-sn-glycero-3-phospho-N,N-dimethylethanolamine, PDME), and PDME to phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine, PC), producing S-adenosyl-L-homocysteine in each step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>1,2-di-(9Z,12Z,15Z-octadecatrienoyl)-sn-glycero-3-phospho-N,N-dimethylethanolamine + S-adenosyl-L-methionine = 1,2-di-(9Z,12Z,15Z-octadecatrienoyl)-sn-glycero-3-phosphocholine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70759"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:86161"/>
            <dbReference type="ChEBI" id="CHEBI:189860"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:70760"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>1,2-di-(9Z,12Z,15Z-octadecatrienoyl)-sn-glycero-3-phospho-N-methylethanolamine + S-adenosyl-L-methionine = 1,2-di-(9Z,12Z,15Z-octadecatrienoyl)-sn-glycero-3-phospho-N,N-dimethylethanolamine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70755"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:189859"/>
            <dbReference type="ChEBI" id="CHEBI:189860"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:70756"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>1,2-di-(9Z,12Z,15Z-octadecatrienoyl)-sn-glycero-3-phosphoethanolamine + S-adenosyl-L-methionine = 1,2-di-(9Z,12Z,15Z-octadecatrienoyl)-sn-glycero-3-phospho-N-methylethanolamine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70751"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:189858"/>
            <dbReference type="ChEBI" id="CHEBI:189859"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:70752"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho-N,N-dimethylethanolamine + S-adenosyl-L-methionine = 1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70747"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:42027"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:189849"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:70748"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho-N-methylethanolamine + S-adenosyl-L-methionine = 1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho-N,N-dimethylethanolamine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70743"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:189848"/>
            <dbReference type="ChEBI" id="CHEBI:189849"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:70744"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphoethanolamine + S-adenosyl-L-methionine = 1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho-N-methylethanolamine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70739"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:172403"/>
            <dbReference type="ChEBI" id="CHEBI:189848"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:70740"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phospho-N,N-dimethylethanolamine + S-adenosyl-L-methionine = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70623"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74669"/>
            <dbReference type="ChEBI" id="CHEBI:85680"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:70624"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phospho-N-methylethanolamine + S-adenosyl-L-methionine = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phospho-N,N-dimethylethanolamine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46112"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85679"/>
            <dbReference type="ChEBI" id="CHEBI:85680"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:46113"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + S-adenosyl-L-methionine = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phospho-N-methylethanolamine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70619"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74986"/>
            <dbReference type="ChEBI" id="CHEBI:85679"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:70620"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phospho-N,N-dimethylethanolamine + S-adenosyl-L-methionine = 1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70771"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74963"/>
            <dbReference type="ChEBI" id="CHEBI:189862"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:70772"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phospho-N-methylethanolamine + S-adenosyl-L-methionine = 1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phospho-N,N-dimethylethanolamine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70767"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:189861"/>
            <dbReference type="ChEBI" id="CHEBI:189862"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:70768"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphoethanolamine + S-adenosyl-L-methionine = 1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phospho-N-methylethanolamine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70763"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:78261"/>
            <dbReference type="ChEBI" id="CHEBI:189861"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:70764"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-N,N-dimethylethanolamine + S-adenosyl-L-methionine = a 1,2-diacyl-sn-glycero-3-phosphocholine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32739"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64572"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine + S-adenosyl-L-methionine = a 1,2-diacyl-sn-glycero-3-phospho-N,N-dimethylethanolamine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32735"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64572"/>
            <dbReference type="ChEBI" id="CHEBI:64573"/>
            <dbReference type="EC" id="2.1.1.71"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + S-adenosyl-L-methionine = a 1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11164"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64573"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="EC" id="2.1.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 27">Phospholipid metabolism; phosphatidylcholine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="27">Endoplasmic reticulum membrane</location>
            <topology evidence="27">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="27">Mitochondrion membrane</location>
            <topology evidence="27">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="27">Found in endoplasmic reticulum where most PEMT activity is generated and in mitochondria.</text>
    </comment>
    <comment type="similarity">
        <text evidence="27">Belongs to the class VI-like SAM-binding methyltransferase superfamily. PEMT/PEM2 methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.17" evidence="27"/>
    <dbReference type="EC" id="2.1.1.71" evidence="27"/>
    <dbReference type="EMBL" id="GU589372">
        <property type="protein sequence ID" value="ADO29322.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187936.1">
        <property type="nucleotide sequence ID" value="NM_001201007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TG86"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000017571"/>
    <dbReference type="GeneID" id="100528737"/>
    <dbReference type="KEGG" id="ipu:100528737"/>
    <dbReference type="CTD" id="10400"/>
    <dbReference type="OMA" id="PTFWNIA"/>
    <dbReference type="OrthoDB" id="8300106at2759"/>
    <dbReference type="UniPathway" id="UPA00753"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000773">
        <property type="term" value="F:phosphatidyl-N-methylethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004608">
        <property type="term" value="F:phosphatidylethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006656">
        <property type="term" value="P:phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1630:FF:000005">
        <property type="entry name" value="Phosphatidylethanolamine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03216">
        <property type="entry name" value="PLMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024960">
        <property type="entry name" value="PEMT/MFAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007318">
        <property type="entry name" value="Phopholipid_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15458">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15458:SF5">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04191">
        <property type="entry name" value="PEMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005444">
        <property type="entry name" value="PEMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51599">
        <property type="entry name" value="SAM_PEMT_PEM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 27" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 27" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10 27" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 27 28" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 27 29" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11 27" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13 27" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="14 27" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 27" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 27 29" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 27 28" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 27 28" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Lumenal" evidence="27">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="intramembrane region" description="Helical" evidence="27">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="27">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="27">
        <location>
            <begin position="96"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="118"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="27">
        <location>
            <begin position="144"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="27">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <begin position="127"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <begin position="209"/>
            <end position="210"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00050814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00050899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00051210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00051451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00051455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00051880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00051941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00052126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00052148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EMBL" id="ADO29322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="RefSeq" id="NP_001187936.1"/>
        </source>
    </evidence>
    <sequence length="227" mass="25453" checksum="C1D70AA674C321AB" modified="2011-01-11" version="1">MNFAAITEINPDCCGGLDKVDFSKADMSVLYEVMKHIDLTEPSFCMAVITIIFNPLFWNVVARWEHRTRGLTRMFKSPYVACYCLGAVILLLNVYRSHSITVAMEGQSKMELLDRVQVYYMGTALMLVGSLFVISSFLALGFTGTFLGDYFGILMEQKVIGFPFNVMDNPMYWGSTANYLGLALMNASPVGVLLSTVVALCYRVAIAYEGPFTEKIYQQRSLSCKHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5U819</accession>
    <name>W5U819_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DDB1- and CUL4-associated factor 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">WD repeat-containing protein 32</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">dcaf10</name>
        <name evidence="13" type="synonym">wdr32</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat DCAF10 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT407284">
        <property type="protein sequence ID" value="AHH37935.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017320121.1">
        <property type="nucleotide sequence ID" value="XM_017464632.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U819"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008586"/>
    <dbReference type="GeneID" id="108263617"/>
    <dbReference type="KEGG" id="ipu:108263617"/>
    <dbReference type="CTD" id="405882"/>
    <dbReference type="GeneTree" id="ENSGT00390000012666"/>
    <dbReference type="OMA" id="STAHEDC"/>
    <dbReference type="OrthoDB" id="20669at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000116">
        <property type="entry name" value="DDB1- and CUL4-associated factor 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001360">
        <property type="entry name" value="DDB1- and CUL4-associated factor 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039085">
        <property type="entry name" value="DCA10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14588">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14588:SF2">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="5 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="168"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="210"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="310"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="13"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="37"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="311"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="326"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AHH37935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017320121.1"/>
        </source>
    </evidence>
    <sequence length="509" mass="57241" checksum="B805731996056A3B" modified="2014-04-16" version="1">MISDQPSDSSDPEEARLIRDPEQLFRKDESEEEEEGKDVRSRPSSPESRHDNSQTQGSSRPESAADGGAPSAGFRTSSVRESLFSWLRNRSVRRGPFVDPVRDNFRTMTRLYSSMSPTTDSMNLSTQTHGAVFNLEYSPDGSVLTVACEQTEVLLFDPVSSRHIKTLVEAHEDCVNNIRFLDNRLFATCSDDTTIALWDLRKLNSKVCSLHGHASWVKNIEYDTHTRLLVTSGFDGNVITWDTNRFTEDGCPHKKFFHTRYLMRMRLTPDCSKMLISTSSGYLLILHDLDLTQSLEVGSYRILRARRAPLSTEGSSTGSRTSGSRHGNDSKAHPHREGLSPRNSLEVLTPEIPGERDRGNCITSLQLHPKGWATLLRCSSNMDDQEWTCVYEFQEGAPPRLPVSPRGSLRLTHYIEEANVGRGYIKELCFSPDGRLICSPYGYGVRLLAFDEHCAELEECVPERTALLREVRSIYSHSDVVLTSKFSPTHCQLASGCLSGRVALYQPHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5UA76</accession>
    <name>W5UA76_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Di-N-acetylchitobiase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CTBS</name>
        <name evidence="15" type="synonym">ctbs</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the degradation of asparagine-linked glycoproteins. Hydrolyze of N-acetyl-beta-D-glucosamine (1-4)N-acetylglucosamine chitobiose core from the reducing end of the bond, it requires prior cleavage by glycosylasparaginase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT408879">
        <property type="protein sequence ID" value="AHH38479.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017333054.1">
        <property type="nucleotide sequence ID" value="XM_017477565.3"/>
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    <dbReference type="AlphaFoldDB" id="W5UA76"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000004533"/>
    <dbReference type="GeneID" id="108270715"/>
    <dbReference type="KEGG" id="ipu:108270715"/>
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    <dbReference type="GeneTree" id="ENSGT00390000012891"/>
    <dbReference type="OMA" id="KWIMKQV"/>
    <dbReference type="OrthoDB" id="73875at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:chitin binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:chitinase activity"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009313">
        <property type="term" value="P:oligosaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02875">
        <property type="entry name" value="GH18_chitobiase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.10:FF:000006">
        <property type="entry name" value="Chitobiase, di-N-acetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000250">
        <property type="entry name" value="Probable di-N-acetylchitobiase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047898">
        <property type="entry name" value="DIAC_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051887">
        <property type="entry name" value="GH18_Domain-Containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46290">
        <property type="entry name" value="DI-N-ACETYLCHITOBIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46290:SF1">
        <property type="entry name" value="DI-N-ACETYLCHITOBIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0458">Lysosome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540108" description="Di-N-acetylchitobiase" evidence="11">
        <location>
            <begin position="19"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="12">
        <location>
            <begin position="1"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AHH38479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017333054.1"/>
        </source>
    </evidence>
    <sequence length="378" mass="42578" checksum="AF86A7DD5C974AEA" modified="2014-04-16" version="1">MWLIVSLVVTLSLSGSSAVCPCEHEQLCQPVQQEHEFEVFVFDVGGKGWKFYDWSHVTTVATFGKYDAELMCYAHSKGARVVLKGVVPLSDMVDVGKRTAWITEKVNLAKSQFMDGINLDIEQAVEESSPEYYALTALVRETTETFHREIRGSQVSFDVAWSPKCIDERCYDYKAIADSCDLVFVMSYDEQSQIWGDCIAMANAPLNQTLAAYDQYMGMKIDSNKLVMGVPWYGYDYQCLNLSQEGICTIPKVPFRGAPCSDASGKQIPYRTMMKQISSSTSGRLWDEIQQAPYYNYQDGKGNIHQVWYDDPESISLKASYVGQRRLRGIGMWNGNLLDYGNDPVARQQSAEMWNALIPKHAPSMTVSYEATTVSPNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5UD98</accession>
    <name>W5UD98_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Testis-expressed protein 264 homolog</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="3">Testis-expressed sequence 264 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TEX264</name>
        <name evidence="5" type="synonym">tex264a</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT412795">
        <property type="protein sequence ID" value="AHH39996.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017335368.1">
        <property type="nucleotide sequence ID" value="XM_017479879.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UD98"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027785"/>
    <dbReference type="GeneID" id="108271919"/>
    <dbReference type="KEGG" id="ipu:108271919"/>
    <dbReference type="CTD" id="402960"/>
    <dbReference type="GeneTree" id="ENSGT00390000016901"/>
    <dbReference type="OMA" id="SPICGAY"/>
    <dbReference type="OrthoDB" id="2140079at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106300">
        <property type="term" value="P:protein-DNA covalent cross-linking repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.80.10">
        <property type="entry name" value="Regulatory factor, effector binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011256">
        <property type="entry name" value="Reg_factor_effector_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15949">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15949:SF3">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55136">
        <property type="entry name" value="Probable bacterial effector-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="269"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AHH39996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017335368.1"/>
        </source>
    </evidence>
    <sequence length="318" mass="35197" checksum="6CED6CA445FC1F7B" modified="2014-04-16" version="1">MSDFVILSLILFLVVCLILTLAGFVFYSGLLSEIIVRTGSPPVKKITIAYKFIKGSYRDRGAAFTESCSIAPKLCSIALYYDNPNEAEADCCRYAVGSILSHDEEKTDEELQRLYEKFGFQVISFPEVSYAVLSSFPNHCMLSPICGAYRVFPELHSYIAERGLSAYPFIEICKGDIIHYMCPLENQGSFFVPELLEKQTEGEEETENDKDADDTHTGEGCTSESGVAVMEADTEISEMAESSLLVMQQSPPLDETDSQTEEGDQGAKGSSESVGSGSSFEELDMDIEEVENEREDVEEDHVEDNEKRGDNEGESNEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>W5UE08</accession>
    <name>W5UE08_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zinc finger CCCH-type with G patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16 18 19" type="primary">zgpat</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18 19" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18 19">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT413314">
        <property type="protein sequence ID" value="AHH40203.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017333192.1">
        <property type="nucleotide sequence ID" value="XM_017477703.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017333193.1">
        <property type="nucleotide sequence ID" value="XM_017477704.3"/>
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    <dbReference type="AlphaFoldDB" id="W5UE08"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019001"/>
    <dbReference type="GeneID" id="108270786"/>
    <dbReference type="KEGG" id="ipu:108270786"/>
    <dbReference type="CTD" id="84619"/>
    <dbReference type="GeneTree" id="ENSGT00390000000732"/>
    <dbReference type="OMA" id="QYTRGIG"/>
    <dbReference type="OrthoDB" id="4822at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="CDD" id="cd20384">
        <property type="entry name" value="Tudor_ZGPAT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46297">
        <property type="entry name" value="ZINC FINGER CCCH-TYPE WITH G PATCH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46297:SF1">
        <property type="entry name" value="ZINC FINGER CCCH-TYPE WITH G PATCH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="172"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="G-patch" evidence="15">
        <location>
            <begin position="316"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="172"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="101"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="371"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="440"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="68"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="380"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AHH40203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017333192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017333193.1"/>
        </source>
    </evidence>
    <sequence length="512" mass="56735" checksum="85C8B90B4F59ACDD" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5UFA5</accession>
    <name>W5UFA5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Sodium/potassium-transporting ATPase subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">atnb233</name>
        <name evidence="22" type="synonym">atp1b1b</name>
    </gene>
    <organism evidence="20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="20">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the X(+)/potassium ATPases subunit beta family.</text>
    </comment>
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        <property type="protein sequence ID" value="AHH40595.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_017456875.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UFA5"/>
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    <dbReference type="KEGG" id="ipu:108258321"/>
    <dbReference type="CTD" id="64273"/>
    <dbReference type="GeneTree" id="ENSGT01030000234579"/>
    <dbReference type="OMA" id="TAYDKNY"/>
    <dbReference type="OrthoDB" id="5912413at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005890">
        <property type="term" value="C:sodium:potassium-exchanging ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000062">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1660">
        <property type="entry name" value="Na, k-atpase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000402">
        <property type="entry name" value="Na/K_ATPase_sub_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038702">
        <property type="entry name" value="Na/K_ATPase_sub_beta_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01107">
        <property type="entry name" value="Na_K_ATPase_bet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11523">
        <property type="entry name" value="SODIUM/POTASSIUM-DEPENDENT ATPASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11523:SF10">
        <property type="entry name" value="SODIUM_POTASSIUM-TRANSPORTING ATPASE SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00287">
        <property type="entry name" value="Na_K-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00391">
        <property type="entry name" value="ATPASE_NA_K_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 19" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="11" id="KW-0915">Sodium</keyword>
    <keyword evidence="16" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6" id="KW-0740">Sodium/potassium transport</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 19" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="35"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00025540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00038795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="AHH40595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017312364.1"/>
        </source>
    </evidence>
    <sequence length="302" mass="34655" checksum="E03F0F0D2A2742AD" modified="2014-04-16" version="1">MPAQNKNDGGWKKSVWNSDTREFLGRTGGSWAKILLFYVIFYAFLAGIFIGTIQALLLTLSNYKPTWQDRVTPPGLTHSPQSDKAGIFFTVNDPTTYLKSIKSIKEFLQAYDVESQRDQLKFEDCGEQPETYKHRGDLESEIGIRKACRFPKSILGPCSGIEDQNFGFTEGRPCLIVKLNRIVNFRPKPPQSNESIPEEVKSKVQPNLIPLHCTTKKKEEEDLLGQVAYYGIGGGFPLQYYPYYGKLLHPHYLQPLVAIQFINITMNQEIRVECRVYGENILYSDKDRYQGRFEVKMKVNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>W5UFF8</accession>
    <name>W5UFF8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4 6">Regulator of G-protein signaling 7-binding protein B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">rgs7bpb</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="4">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RGS7BP/RGS9BP family.</text>
    </comment>
    <dbReference type="EMBL" id="JT408635">
        <property type="protein sequence ID" value="AHH38412.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053543755.1">
        <property type="nucleotide sequence ID" value="XM_053687780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UFF8"/>
    <dbReference type="GeneID" id="128635335"/>
    <dbReference type="KEGG" id="ipu:128635335"/>
    <dbReference type="CTD" id="337556"/>
    <dbReference type="GeneTree" id="ENSGT00940000153725"/>
    <dbReference type="OMA" id="QTRSKGC"/>
    <dbReference type="OrthoDB" id="9876293at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026512">
        <property type="entry name" value="RGS7BP/RGS9BP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21029">
        <property type="entry name" value="R-SEVEN BINDING PROTEIN (R7BP) HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0734">Signal transduction inhibitor</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AHH38412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_053543755.1"/>
        </source>
    </evidence>
    <sequence length="252" mass="28314" checksum="251FEF5965F522EA" modified="2014-04-16" version="1">MCSAPNGRKSRPGSAGRMFQLGKAARSEPERRESTDSARRGGQRAVEHCRAAVQEFNTLVALHREQVISIGEITADCPTLRARMHSTRTKGCTVAESAYQHLTLISGPEDGEIHPEICRLFIQLQCCLEMYITEMLKSMCLLGVLQLHRKGNEPCPESNADCRLDESSDVPILEDRSSSPVDFPHESWLVCTDIENIESDMREMRNLLSKLRETMPLPLKNQDDSSLLNLTPYPLVRQRKRRFSGLCCLVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>W5UIZ2</accession>
    <name>W5UIZ2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6 8">Claudin domain-containing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CLDND1</name>
        <name evidence="8" type="synonym">cldnd1a</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT415359">
        <property type="protein sequence ID" value="AHH41660.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017316772.1">
        <property type="nucleotide sequence ID" value="XM_017461283.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UIZ2"/>
    <dbReference type="GeneID" id="108260762"/>
    <dbReference type="CTD" id="335628"/>
    <dbReference type="GeneTree" id="ENSGT00390000002596"/>
    <dbReference type="OrthoDB" id="9885915at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042356">
        <property type="entry name" value="CLDN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14347">
        <property type="entry name" value="CLAUDIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14347:SF3">
        <property type="entry name" value="CLAUDIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13903">
        <property type="entry name" value="Claudin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="214"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AHH41660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017316772.1"/>
        </source>
    </evidence>
    <sequence length="253" mass="28281" checksum="B5A16058E693F0BE" modified="2014-04-16" version="1">MVDNRSATALVIACVLSALATVYLSVAVGTRHWYQYTSPQVRHEHNATELRALHQELSDGEFDEKTASDAMFRLNGTLGLWWRCVRVPADRNRWYREPDPEMDTECVSFTLSQQFTPKYTEPGNHNSGEDIIRTYMWRCQFLLPLLSLGLVLVGGLVGFCACLCRSFSPTLGIGLLHLLAGVCSLGCVCCFLAGMDLLHRVSVIPDQVDGSLGWSLYLALISSPLYMMAAALLVWAARSHSKNYYRFTAYRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5UJR4</accession>
    <name>W5UJR4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19 21 22" type="primary">pkmyt1</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21 22" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21 22">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JT412615">
        <property type="protein sequence ID" value="AHH39922.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017339116.1">
        <property type="nucleotide sequence ID" value="XM_017483627.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017339118.1">
        <property type="nucleotide sequence ID" value="XM_017483629.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UJR4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030730"/>
    <dbReference type="GeneID" id="108273928"/>
    <dbReference type="KEGG" id="ipu:108273928"/>
    <dbReference type="CTD" id="9088"/>
    <dbReference type="OMA" id="PCTPPKD"/>
    <dbReference type="OrthoDB" id="5337378at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110031">
        <property type="term" value="P:negative regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000315">
        <property type="entry name" value="membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF183">
        <property type="entry name" value="MEMBRANE-ASSOCIATED TYROSINE- AND THREONINE-SPECIFIC CDC2-INHIBITORY KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 19 21" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="105"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="474"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="474"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AHH39922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017339116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017339118.1"/>
        </source>
    </evidence>
    <sequence length="528" mass="58681" checksum="3C5DB926BBE58DF0" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5UK54</accession>
    <name>W5UK54_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7 9">Tropomodulin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TMOD1</name>
        <name evidence="9" type="synonym">tmod1</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tropomodulin family.</text>
    </comment>
    <dbReference type="EMBL" id="JT417347">
        <property type="protein sequence ID" value="AHH42351.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017316623.1">
        <property type="nucleotide sequence ID" value="XM_017461134.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UK54"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033171"/>
    <dbReference type="GeneID" id="108260688"/>
    <dbReference type="CTD" id="7111"/>
    <dbReference type="GeneTree" id="ENSGT00940000158695"/>
    <dbReference type="OMA" id="PYQRDKL"/>
    <dbReference type="OrthoDB" id="2163268at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005865">
        <property type="term" value="C:striated muscle thin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030239">
        <property type="term" value="P:myofibril assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051694">
        <property type="term" value="P:pointed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000006">
        <property type="entry name" value="Tropomodulin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004934">
        <property type="entry name" value="TMOD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10901">
        <property type="entry name" value="TROPOMODULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10901:SF8">
        <property type="entry name" value="TROPOMODULIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03250">
        <property type="entry name" value="Tropomodulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AHH42351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017316623.1"/>
        </source>
    </evidence>
    <sequence length="357" mass="40654" checksum="5172244074B85CDD" modified="2014-04-16" version="1">MSYRKEMEKYRDVDEDELLQKLSEEELRRLEDELEELDPDNALLPAGMRQRDQTKKAPTGTFHRDNLLAHLEKQAKEHPDRDDLVPYTGEKRGKPWVPKTKVIDPILENVTLEPELEEALSNATDAELCDIAAILGMHTLMSNQQYYEALASSTIVNKQGLNSVIQCAQYKQIPDEHPNDTDVEQTLMKIKHNDPELVEVNLNNIKNIPITTLKAYAETLKENTVVERLSIVGTRSNDPVAYAFADMLRVNTTLKCLNVESNFITGNGVLALVESLQYNTTLQELKIDNQSQPLGNKVEMEIASMLEKNSTLLKFGYHFTQQGPRLRCSNAMMNNNDLVRKKRRESGPIFPKCRTDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5UKK3</accession>
    <name>W5UKK3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">45 kDa calcium-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Stromal cell-derived factor 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13 15" type="primary">sdf4</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Golgi apparatus lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CREC family.</text>
    </comment>
    <dbReference type="EMBL" id="JT416998">
        <property type="protein sequence ID" value="AHH42225.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017342495.1">
        <property type="nucleotide sequence ID" value="XM_017487006.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UKK3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032926"/>
    <dbReference type="GeneID" id="108275905"/>
    <dbReference type="KEGG" id="ipu:108275905"/>
    <dbReference type="CTD" id="51150"/>
    <dbReference type="GeneTree" id="ENSGT01010000222360"/>
    <dbReference type="OMA" id="MNEYSAL"/>
    <dbReference type="OrthoDB" id="9978834at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005796">
        <property type="term" value="C:Golgi lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017156">
        <property type="term" value="P:calcium-ion regulated exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16225">
        <property type="entry name" value="EFh_CREC_cab45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000120">
        <property type="entry name" value="45 kDa calcium-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000207">
        <property type="entry name" value="Putative 45 kDa calcium-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027240">
        <property type="entry name" value="CAB45_EFh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827:SF98">
        <property type="entry name" value="45 KDA CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827">
        <property type="entry name" value="RETICULOCALBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540429" description="45 kDa calcium-binding protein" evidence="11">
        <location>
            <begin position="30"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="92"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="131"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="232"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="272"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AHH42225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017342495.1"/>
        </source>
    </evidence>
    <sequence length="356" mass="41786" checksum="EF63DD4304204722" modified="2014-04-16" version="1">MPSTRGVRTGHRGAVFFALFVILIMDVQTRPANISVHRNKHATGKEDNEILPPDHLNGVKMEMDGHLNKDFHQEVFLGKEMEEFDEDSEPKKNRKKLIEIFSKVDINKDRSVSAKELQRWIMEKTEEHFQEAVKENKLNFRAVDPDGDGHVTWDEYRVKFLASKGFNEKEVAEKIKNNEELKVDEETQEILESLKDRWFQADNPPADQLLNEEEFLSFLHPEHSRGMLKYMVKEIIRDLDQDGDKELTLSEFISLPMGTVENQQGQDIDDDWVRERKKEFEEVIDANHNGIVTMEELEEYMDPMNEYNALNEAKQMIAVADENQNHNLELEEILKYSEYFTGSKFMDYARNVHEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>W5URF4</accession>
    <name>W5URF4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP-1 complex subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">AP1G1</name>
        <name evidence="14" type="synonym">ap1g1</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endomembrane system</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="JT416933">
        <property type="protein sequence ID" value="AHH42198.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053539368.1">
        <property type="nucleotide sequence ID" value="XM_053683393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5URF4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008297"/>
    <dbReference type="GeneID" id="108270696"/>
    <dbReference type="KEGG" id="ipu:108270696"/>
    <dbReference type="OMA" id="AICAMRI"/>
    <dbReference type="OrthoDB" id="28053at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000030">
        <property type="entry name" value="AP-1 complex subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050840">
        <property type="entry name" value="Adaptor_Complx_Large_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017107">
        <property type="entry name" value="AP1_complex_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008153">
        <property type="entry name" value="GAE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22780">
        <property type="entry name" value="ADAPTIN, ALPHA/GAMMA/EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02883">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037094">
        <property type="entry name" value="AP1_complex_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00809">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50180">
        <property type="entry name" value="GAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="GAE" evidence="11">
        <location>
            <begin position="697"/>
            <end position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AHH42198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_053539368.1"/>
        </source>
    </evidence>
    <sequence length="817" mass="90917" checksum="EA7653ECCBA7ABAC" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PJ25</accession>
    <name>A0A2D0PJ25_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nuclear transcription factor Y subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">LOC108254946</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8 9">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NFYA/HAP2 subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017305961.1">
        <property type="nucleotide sequence ID" value="XM_017450472.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017305962.1">
        <property type="nucleotide sequence ID" value="XM_017450473.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PJ25"/>
    <dbReference type="GeneID" id="108254946"/>
    <dbReference type="KEGG" id="ipu:108254946"/>
    <dbReference type="OrthoDB" id="1097733at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001289">
        <property type="entry name" value="NFYA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12632">
        <property type="entry name" value="TRANSCRIPTION FACTOR NF-Y ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02045">
        <property type="entry name" value="CBFB_NFYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00521">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51152">
        <property type="entry name" value="NFYA_HAP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017305961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017305962.1"/>
        </source>
    </evidence>
    <sequence length="310" mass="32477" checksum="0987C9E4079AE803" modified="2017-12-20" version="1">MEQFAESTSGGEQIVVQASSGAVQQQSTVTAVQLQADGQVDAGAGQQVQTLQVVEGQPLMVQVSGGQLITPSGQPIMVQAMPGGQAQTIMQLPVTGSQGLQQIQLVQPGQIQLSGGQTLQVQTAQGQTQQIIIQQPQTAVTAGQNQPQQITVQGQQAQTAEGQTILYQPVNADGTILQQGVITIPAASLAGGQIIQTGSNASGTGGQGTVTVAVPVTGNMVNAGGMVMMVQSGGSVPTMQRIPLPGTEMLEEEPLYVNPKQYHRILKRRQARAKLEAEGKIPKRRRVNVKPRVGIVTARTSLLKDEETKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PJ40</accession>
    <name>A0A2D0PJ40_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein FAM107B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">si:ch211-160o17.6</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017306598.1">
        <property type="nucleotide sequence ID" value="XM_017451109.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PJ40"/>
    <dbReference type="GeneID" id="108255272"/>
    <dbReference type="KEGG" id="ipu:108255272"/>
    <dbReference type="OrthoDB" id="5963205at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009533">
        <property type="entry name" value="FAM107"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16768">
        <property type="entry name" value="DOWN REGULATED IN RENAL CARCINOMA 1/TU3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16768:SF7">
        <property type="entry name" value="PROTEIN FAM107B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06625">
        <property type="entry name" value="DUF1151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017306598.1"/>
        </source>
    </evidence>
    <sequence length="176" mass="21129" checksum="388355BA4CF864A7" modified="2017-12-20" version="1">MCLRVTSSLLKKSMLLYFVPQMVSPRNVERNDIAENSRSETVAQEQIMENHDHLIRPKKPSNPVLESPRHRVLHRELRVSHRWGLLPAEKCELQRVMEQRRLEQQREREQAQRPLSDLDQQLSRRRQRLLAYELEEQKRQEDRQNVPEFVRVKDNLRRVCVSENESRSFGLRLQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PKD6</accession>
    <name>A0A2D0PKD6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Kelch-like protein 10 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC108255562</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017307088.1">
        <property type="nucleotide sequence ID" value="XM_017451599.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PKD6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034661"/>
    <dbReference type="GeneID" id="108255562"/>
    <dbReference type="KEGG" id="ipu:108255562"/>
    <dbReference type="OMA" id="SEWHAVT"/>
    <dbReference type="OrthoDB" id="191037at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="CDD" id="cd18450">
        <property type="entry name" value="BACK_KLHL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF172">
        <property type="entry name" value="KELCH-LIKE PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00501">
        <property type="entry name" value="KELCHREPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="31"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017307088.1"/>
        </source>
    </evidence>
    <sequence length="572" mass="64785" checksum="8F45937B9C6FD6CE" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PKS8</accession>
    <name>A0A2D0PKS8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Hydroxyacylglutathione hydrolase, mitochondrial</fullName>
            <ecNumber evidence="7">3.1.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Glyoxalase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC108254978</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(R)-S-lactoylglutathione + H2O = (R)-lactate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25245"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16004"/>
            <dbReference type="ChEBI" id="CHEBI:57474"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="EC" id="3.1.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:25246"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>an S-(2-hydroxyacyl)glutathione + H2O = a 2-hydroxy carboxylate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21864"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58896"/>
            <dbReference type="ChEBI" id="CHEBI:71261"/>
            <dbReference type="EC" id="3.1.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:21865"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.6" evidence="7"/>
    <dbReference type="RefSeq" id="XP_017306021.1">
        <property type="nucleotide sequence ID" value="XM_017450532.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PKS8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007608"/>
    <dbReference type="GeneID" id="108254978"/>
    <dbReference type="KEGG" id="ipu:108254978"/>
    <dbReference type="OMA" id="NYIWLLQ"/>
    <dbReference type="OrthoDB" id="515692at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004416">
        <property type="term" value="F:hydroxyacylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019243">
        <property type="term" value="P:methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07723">
        <property type="entry name" value="hydroxyacylglutathione_hydrolase_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000019">
        <property type="entry name" value="Hydroxyacylglutathione hydrolase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01374">
        <property type="entry name" value="Glyoxalase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035680">
        <property type="entry name" value="Clx_II_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032282">
        <property type="entry name" value="HAGH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017782">
        <property type="entry name" value="Hydroxyacylglutathione_Hdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03413">
        <property type="entry name" value="GSH_gloB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935">
        <property type="entry name" value="BETA LACTAMASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935:SF80">
        <property type="entry name" value="HYDROXYACYLGLUTATHIONE HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16123">
        <property type="entry name" value="HAGH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005457">
        <property type="entry name" value="Glx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 21" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="19">
        <location>
            <begin position="58"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="284"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="293"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00039338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017306021.1"/>
        </source>
    </evidence>
    <sequence length="307" mass="34176" checksum="891F98D7E53381C4" modified="2017-12-20" version="1">MLFKSALFGACGFGALGVAAYKFAPSQIPAVFFHSVVRKSALVEQSNMKIELLPALTDNYMYLLIDEDTKEAAIVDPVEPGKVVEAVKKHGVKLKSILTTHHHWDHAGGNEKMVKLVPGLTVYGGDDRIGALTKKVTHYNTFKVGTLNVKCLFTPCHTSGHICYFVTSENSSEPPAVFTGDTLFVAGCGKFFEGTADEMYKALVDILGRLPPETRVYCGHEYTINNLKFARHVEPNNEAIRRKLSWAKEKYDNGEPTIPSTMADELTFNPFMRVREKTVQMHAGTNDPIDTMRSIRKEKDSFKVPKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PLH8</accession>
    <name>A0A2D0PLH8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Potassium voltage-gated channel subfamily F member 1a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">kcnf1a</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017307453.1">
        <property type="nucleotide sequence ID" value="XM_017451964.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PLH8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005337"/>
    <dbReference type="GeneID" id="108255764"/>
    <dbReference type="KEGG" id="ipu:108255764"/>
    <dbReference type="CTD" id="557280"/>
    <dbReference type="OMA" id="VIFYSKQ"/>
    <dbReference type="OrthoDB" id="296522at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000005">
        <property type="entry name" value="potassium voltage-gated channel subfamily G member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000091">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003971">
        <property type="entry name" value="K_chnl_volt-dep_Kv5/Kv9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF171">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY F MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01494">
        <property type="entry name" value="KV9CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="169"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="347"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="378"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel tetramerisation-type BTB" evidence="17">
        <location>
            <begin position="18"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="16">
        <location>
            <begin position="173"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="460"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="117"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017307453.1"/>
        </source>
    </evidence>
    <sequence length="483" mass="54070" checksum="24793BBEDE37D0EB" modified="2017-12-20" version="1">MWATPRRTRSESSEDAEIVVNVGGARHVLCASVLERYPATRLSHLAERAAESSCDLSRFCDDYDAATQEFFFDRDPDAFRCIAEVYQYGEVHMARGTCPVRFSKEMEFWGVDMDLLDECCKSNLKEAEDELSEIAEKVRAILDDMEGSPSSGKCRAFLWRLMEKPESSWAARTVALLSFLFVLLSSVVMCVGTVPELHEQDDDGNRVENPSLEAIETACIIWFTVEYVLRLAAAPSKLHFAISFMNVVDFLAIAPFYLLLLLSYLGTAVLQLATVQQAVQALRVMRVARVLKLARHSSGLQTLTYALRSSCKELGLLLMYMGIGIFLFSALGYTLEQSHPETMFTSIPQSFWWAIITMTTVGYGDIYPKTTLGRCNAAVSFLCGVIAIALPIHPIINNFVIFYSKQRVLETAAKHEIELMELRSKEGEPVNAAGLEFQSSSQLMGDRVVWDSANLNSQSDGHIPLFEDDGQSTPFQKCKKRDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PMI3</accession>
    <name>A0A2D0PMI3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Alpha-L-iduronidase isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">idua</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 39 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017307219.1">
        <property type="nucleotide sequence ID" value="XM_017451730.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PMI3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035011"/>
    <dbReference type="GeneID" id="108255637"/>
    <dbReference type="KEGG" id="ipu:108255637"/>
    <dbReference type="CTD" id="3425"/>
    <dbReference type="OMA" id="RYETWNE"/>
    <dbReference type="OrthoDB" id="15153at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003940">
        <property type="term" value="F:L-iduronidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1500:FF:000002">
        <property type="entry name" value="Iduronidase alpha-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1500">
        <property type="entry name" value="Glycosyl hydrolase domain, family 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049165">
        <property type="entry name" value="GH39_as"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049167">
        <property type="entry name" value="GH39_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049166">
        <property type="entry name" value="GH39_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000514">
        <property type="entry name" value="Glyco_hydro_39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051923">
        <property type="entry name" value="Glycosyl_Hydrolase_39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12631">
        <property type="entry name" value="ALPHA-L-IDURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12631:SF8">
        <property type="entry name" value="ALPHA-L-IDURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01229">
        <property type="entry name" value="Glyco_hydro_39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21200">
        <property type="entry name" value="Glyco_hydro_39_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00745">
        <property type="entry name" value="GLHYDRLASE39"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01027">
        <property type="entry name" value="GLYCOSYL_HYDROL_F39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012926187" evidence="5">
        <location>
            <begin position="24"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolases family 39 N-terminal catalytic" evidence="6">
        <location>
            <begin position="33"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-L-iduronidase C-terminal" evidence="7">
        <location>
            <begin position="552"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600514-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017307219.1"/>
        </source>
    </evidence>
    <sequence length="641" mass="72847" checksum="AE2A4630538421D8" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PRF7</accession>
    <name>A0A2D0PRF7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein-lysine N-methyltransferase EEF2KMT</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">eef2kmt</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. EEF2KMT family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017308604.1">
        <property type="nucleotide sequence ID" value="XM_017453115.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PRF7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022979"/>
    <dbReference type="GeneID" id="108256352"/>
    <dbReference type="KEGG" id="ipu:108256352"/>
    <dbReference type="CTD" id="196483"/>
    <dbReference type="OMA" id="PIRTYRI"/>
    <dbReference type="OrthoDB" id="194386at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029426">
        <property type="entry name" value="FAM86_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF130">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE EEF2KMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14904">
        <property type="entry name" value="FAM86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAM86 N-terminal" evidence="5">
        <location>
            <begin position="12"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017308604.1"/>
        </source>
    </evidence>
    <sequence length="320" mass="35881" checksum="1D303882224F2E2A" modified="2017-12-20" version="1">MSIKTVDVPCLFQLSFFAMCRLHTFPWRYLETELQNSPSEVILDILKKTCLHPVCHKTPPSVKYRRLFLSELIKRYESTASEPLDELYDALAEVLGAAEETACYKTYFLPSGEPVSLEENVAMISEGTTGLVTWEAALYLAEWALENTHIFTGRTVLELGSGAGLTGVAVCHACKPKKYIFTDCHQSVLQRLQNNVQHNGLNEEDGTSVSVCVGELDWEHARDEELQRFGAQTIIAADVVYDPEIVVCLVRLLAKLLKIQKKPPEVYISSTIRNPGTYTCFKKELEKAGLKHVIVTNPVTQVFLYDRVSSIEIINVHAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PTU1</accession>
    <name>A0A2D0PTU1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ribosome biogenesis protein C1orf109 homolog isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">c19h1orf109</name>
        <name evidence="3" type="synonym">c24h1orf109</name>
        <name evidence="3" type="synonym">ca109</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017309427.1">
        <property type="nucleotide sequence ID" value="XM_017453938.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PTU1"/>
    <dbReference type="GeneID" id="100528479"/>
    <dbReference type="CTD" id="54955"/>
    <dbReference type="OrthoDB" id="6605214at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029159">
        <property type="entry name" value="CA109-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16234:SF5">
        <property type="entry name" value="AFG2-INTERACTING RIBOSOME MATURATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16234">
        <property type="entry name" value="SIMILAR TO HYPOTHETICAL PROTEIN FLJ20508"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15011">
        <property type="entry name" value="CA109-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017309427.1"/>
        </source>
    </evidence>
    <sequence length="198" mass="22550" checksum="91666445CD3C9418" modified="2017-12-20" version="1">MSQPALLQFHQELRKCFQSLKANQTVWNGVLEECTPLLSSLGNLAEQLRALKSVEIANTPLSTFPNLPERLQHKLLNAVDTVLGELSEKVNALDLMRASVCKQVAAVFQMYEQNSELLPISTCVARCALSPSIADMLEWLQDTERYYRMQCIQRKNLLQLLKPDDLTLLETAPKRWTSLDSPRGEDRISDPSPWQQWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PUE5</accession>
    <name>A0A2D0PUE5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cystatin-B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Stefin-B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC108256405</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cystatin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017308724.1">
        <property type="nucleotide sequence ID" value="XM_017453235.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PUE5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000016491"/>
    <dbReference type="GeneID" id="108256405"/>
    <dbReference type="KEGG" id="ipu:108256405"/>
    <dbReference type="OMA" id="PCNGGET"/>
    <dbReference type="OrthoDB" id="2429551at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071220">
        <property type="term" value="P:cellular response to bacterial lipoprotein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.10:FF:000001">
        <property type="entry name" value="Cystatin-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018073">
        <property type="entry name" value="Prot_inh_cystat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001713">
        <property type="entry name" value="Prot_inh_stefin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11414:SF21">
        <property type="entry name" value="CYSTATIN 14A, TANDEM DUPLICATE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11414">
        <property type="entry name" value="CYSTATIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00031">
        <property type="entry name" value="Cystatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00295">
        <property type="entry name" value="STEFINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00043">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00287">
        <property type="entry name" value="CYSTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0789">Thiol protease inhibitor</keyword>
    <feature type="domain" description="Cystatin" evidence="9">
        <location>
            <begin position="3"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017308724.1"/>
        </source>
    </evidence>
    <sequence length="105" mass="11878" checksum="96A1E300EE5A98F6" modified="2017-12-20" version="1">MPLLRGGTTETKDATDEVQKICDEVKPHAEEKAGKTFDVFTAKSYKTQVVAGTNYFIKVHVGGEDFVHLRVYKMLPCAGEKLELTGIQTPKAHHDPIEYFEHMFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PVG9</accession>
    <name>A0A2D0PVG9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein fasn2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">fasn2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>holo-[ACP] + malonyl-CoA = malonyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="EC" id="2.3.1.39"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:41793"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017309416.1">
        <property type="nucleotide sequence ID" value="XM_017453927.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000019117"/>
    <dbReference type="GeneID" id="108256736"/>
    <dbReference type="KEGG" id="ipu:108256736"/>
    <dbReference type="CTD" id="100000781"/>
    <dbReference type="OrthoDB" id="329835at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004314">
        <property type="term" value="F:[acyl-carrier-protein] S-malonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05274">
        <property type="entry name" value="KR_FAS_SDR_x"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00833">
        <property type="entry name" value="PKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.250">
        <property type="entry name" value="Malonyl-CoA ACP transacylase, ACP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.110">
        <property type="entry name" value="Polyketide synthase dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032821">
        <property type="entry name" value="PKS_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042104">
        <property type="entry name" value="PKS_dehydratase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020807">
        <property type="entry name" value="PKS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049551">
        <property type="entry name" value="PKS_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049552">
        <property type="entry name" value="PKS_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013968">
        <property type="entry name" value="PKS_KR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049900">
        <property type="entry name" value="PKS_mFAS_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050444">
        <property type="entry name" value="Polyketide_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45681:SF8">
        <property type="entry name" value="CARRIER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45681">
        <property type="entry name" value="POLYKETIDE SYNTHASE 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16197">
        <property type="entry name" value="KAsynt_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08659">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21089">
        <property type="entry name" value="PKS_DH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14765">
        <property type="entry name" value="PS-DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00826">
        <property type="entry name" value="PKS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52019">
        <property type="entry name" value="PKS_MFAS_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="8">
        <location>
            <begin position="5"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="PKS/mFAS DH" evidence="9">
        <location>
            <begin position="883"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="7">
        <location>
            <begin position="2007"/>
            <end position="2082"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal hotdog fold" evidence="6">
        <location>
            <begin position="883"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal hotdog fold" evidence="6">
        <location>
            <begin position="1028"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for dehydratase activity" evidence="6">
        <location>
            <position position="921"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for dehydratase activity" evidence="6">
        <location>
            <position position="1091"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS52019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017309416.1"/>
        </source>
    </evidence>
    <sequence length="2102" mass="233516" checksum="C476E9E41FDE4355" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PXA2</accession>
    <name>A0A2D0PXA2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein PET100 homolog, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC108257119</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PET100 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017310061.1">
        <property type="nucleotide sequence ID" value="XM_017454572.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PXA2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008875"/>
    <dbReference type="GeneID" id="108257119"/>
    <dbReference type="KEGG" id="ipu:108257119"/>
    <dbReference type="OMA" id="MALYMTF"/>
    <dbReference type="OrthoDB" id="18175at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018625">
        <property type="entry name" value="Pet100"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33968">
        <property type="entry name" value="PROTEIN PET100 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33968:SF1">
        <property type="entry name" value="PROTEIN PET100 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09803">
        <property type="entry name" value="Pet100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017310061.1"/>
        </source>
    </evidence>
    <sequence length="78" mass="9803" checksum="3CD20BFC61355FE7" modified="2017-12-20" version="1">MGVKIEIFRMMVYLSFPVTMFWISNQAEYFEEYIVKRKREIFPPDEKMQRKELEEFKERMRIRKEQKMLKKLAMETEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PYX3</accession>
    <name>A0A2D0PYX3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Visinin-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">vsnl1a</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds three calcium ions.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Regulates (in vitro) the inhibition of rhodopsin phosphorylation in a calcium-dependent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the recoverin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017311227.2">
        <property type="nucleotide sequence ID" value="XM_017455738.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PYX3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000010844"/>
    <dbReference type="GeneID" id="108257746"/>
    <dbReference type="KEGG" id="ipu:108257746"/>
    <dbReference type="CTD" id="492494"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000009">
        <property type="entry name" value="Visinin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF101">
        <property type="entry name" value="VISININ-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="60"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="96"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="146"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017311227.2"/>
        </source>
    </evidence>
    <sequence length="191" mass="22185" checksum="B4A81B7E4C72F64A" modified="2023-06-28" version="2">MGKQNSKLTPEVMDDLVKNTEFNEHELKQWYKGFLKDCPSGRLNLDEFQQLYVKFFPYGDASKFAQHAFRTFDRNGDGTIDFREFICALSITSRGSFEQKLNWAFNMYDLDGDGKITRVEMLEIIEAIYKMVGTVIMMKMNEDGLTPEQRVDKIFSKMDKNNDDQISLEEFKEAAKSDPSIVLLLQCDLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q2D5</accession>
    <name>A0A2D0Q2D5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Hepatitis A virus cellular receptor 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC108258075</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the immunoglobulin superfamily. TIM family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017311851.2">
        <property type="nucleotide sequence ID" value="XM_017456362.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q2D5"/>
    <dbReference type="GeneID" id="108258075"/>
    <dbReference type="KEGG" id="ipu:108258075"/>
    <dbReference type="OrthoDB" id="434099at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043277">
        <property type="term" value="P:apoptotic cell clearance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060097">
        <property type="term" value="P:cytoskeletal rearrangement involved in phagocytosis, engulfment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000774">
        <property type="entry name" value="Hepatitis A virus cellular receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46608">
        <property type="entry name" value="T-CELL IMMUNOGLOBULIN AND MUCIN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46608:SF3">
        <property type="entry name" value="T-CELL IMMUNOGLOBULIN AND MUCIN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039940559" evidence="11">
        <location>
            <begin position="23"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="502"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="31"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017311851.2"/>
        </source>
    </evidence>
    <sequence length="563" mass="61857" checksum="199C010845C273C6" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q3Q7</accession>
    <name>A0A2D0Q3Q7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sodium/mannose cotransporter SLC5A10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Sodium/glucose cotransporter 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Solute carrier family 5 member 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">slc5a10</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Electrogenic Na+-coupled sugar symporter that actively transports D-mannose or D-fructose at the plasma membrane, with a Na+ to sugar coupling ratio of 1:1. Transporter activity is driven by a transmembrane Na+ electrochemical gradient set by the Na+/K+ pump. Exclusively recognizes sugar substrates having a pyranose ring with an axial hydroxyl group on carbon 2. Has likely evolved to enable renal reabsorption of D-mannose, an important constituent of oligosaccharide chains of glycoproteins. Contributes to dietary D-fructose reabsorption from glomerular filtrate across the brush border of the kidney.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>D-fructopyranose(out) + Na(+)(out) = D-fructopyranose(in) + Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72915"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:37714"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:72916"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>D-mannose(out) + Na(+)(out) = D-mannose(in) + Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72907"/>
            <dbReference type="ChEBI" id="CHEBI:4208"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:72908"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017313348.1">
        <property type="nucleotide sequence ID" value="XM_017457859.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q3Q7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007886"/>
    <dbReference type="GeneID" id="108258883"/>
    <dbReference type="KEGG" id="ipu:108258883"/>
    <dbReference type="CTD" id="125206"/>
    <dbReference type="OMA" id="LFGGMWS"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005412">
        <property type="term" value="F:D-glucose:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1730.10:FF:000004">
        <property type="entry name" value="sodium/glucose cotransporter 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018212">
        <property type="entry name" value="Na/solute_symporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11819:SF128">
        <property type="entry name" value="SODIUM_MANNOSE COTRANSPORTER SLC5A10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11819">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00456">
        <property type="entry name" value="NA_SOLUT_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="139"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="169"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="300"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="442"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="471"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="513"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="613"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
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</entry>
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        <submittedName>
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        <name evidence="19" type="primary">itga6b</name>
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        <name type="scientific">Ictalurus punctatus</name>
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    </organism>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
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                <person name="Li Z."/>
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                <person name="Feng J."/>
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                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="18">SDA103</strain>
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    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Single-pass type I membrane protein</topology>
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    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the integrin alpha chain family.</text>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
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        <property type="entry name" value="INTEGRIN ALPHA-6"/>
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        <property type="entry name" value="INTEGRINA"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
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    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
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    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5011815648" evidence="13">
        <location>
            <begin position="23"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1008"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="29"/>
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        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="243"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="301"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="363"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="12">
        <location>
            <begin position="421"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha first immunoglubulin-like" evidence="15">
        <location>
            <begin position="468"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha second immunoglobulin-like" evidence="16">
        <location>
            <begin position="630"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha third immunoglobulin-like" evidence="17">
        <location>
            <begin position="786"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="871"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1056"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="871"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1076"/>
            <end position="1087"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08441"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF20805"/>
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    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF20806"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017312927.1"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q4M5</accession>
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        <submittedName>
            <fullName evidence="8">CMRF35-like molecule 7</fullName>
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        <name evidence="8" type="primary">LOC108258815</name>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017313249.1">
        <property type="nucleotide sequence ID" value="XM_017457760.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q4M5"/>
    <dbReference type="GeneID" id="108258815"/>
    <dbReference type="KEGG" id="ipu:108258815"/>
    <dbReference type="OrthoDB" id="8959642at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05716">
        <property type="entry name" value="IgV_pIgR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF118">
        <property type="entry name" value="CMRF35-LIKE MOLECULE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012700231" evidence="5">
        <location>
            <begin position="22"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin V-set" evidence="6">
        <location>
            <begin position="34"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017313249.1"/>
        </source>
    </evidence>
    <sequence length="217" mass="24494" checksum="8E9EF17A044FD8E2" modified="2017-12-20" version="1">MEMKKKCFSTSFILLTVSVMGAMLNDEVHEVGIEDGAAVIKCHYTASYQPYPKYFCKGIYEDCKTLIKSDSRNPWTFEGRLSLFDDTEKSMFVVNINNLSLGDHGKYGCVVNITEQDLFTVVHLTVRKDSRFTTEERPISTVTSRQGNASDSTGSSSQPTVSISLSEQLKAVQTQHCPIQQRTWMSTSMRRHSAATLQKKMPQKPQTECPVRILLQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q4Y1</accession>
    <name>A0A2D0Q4Y1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SH3 and PX domain-containing protein 2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">sh3pxd2aa</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Podosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SH3PXD2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017313352.1">
        <property type="nucleotide sequence ID" value="XM_017457863.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q4Y1"/>
    <dbReference type="GeneID" id="108258886"/>
    <dbReference type="KEGG" id="ipu:108258886"/>
    <dbReference type="CTD" id="562018"/>
    <dbReference type="OrthoDB" id="10255964at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002102">
        <property type="term" value="C:podosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016176">
        <property type="term" value="F:superoxide-generating NADPH oxidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042554">
        <property type="term" value="P:superoxide anion generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06888">
        <property type="entry name" value="PX_FISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12074">
        <property type="entry name" value="SH3_Tks5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12077">
        <property type="entry name" value="SH3_Tks5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12019">
        <property type="entry name" value="SH3_Tks5_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12020">
        <property type="entry name" value="SH3_Tks5_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000020">
        <property type="entry name" value="SH3 and PX domain-containing protein 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000031">
        <property type="entry name" value="SH3 and PX domain-containing protein 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000042">
        <property type="entry name" value="SH3 and PX domain-containing protein 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000059">
        <property type="entry name" value="SH3 and PX domain-containing protein 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000141">
        <property type="entry name" value="SH3 and PX domain-containing protein 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000005">
        <property type="entry name" value="SH3 and PX domain-containing protein 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051228">
        <property type="entry name" value="NADPH_Oxidase/PX-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001655">
        <property type="entry name" value="P47PHOX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037961">
        <property type="entry name" value="SH3PXD2_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035450">
        <property type="entry name" value="SH3PXD2A_SH3_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035452">
        <property type="entry name" value="SH3PXD2A_SH3_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035453">
        <property type="entry name" value="SH3PXD2A_SH3_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035454">
        <property type="entry name" value="SH3PXD2A_SH3_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706:SF2">
        <property type="entry name" value="SH3 AND PX DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706">
        <property type="entry name" value="SH3 MULTIPLE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00498">
        <property type="entry name" value="P47PHOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="PX" evidence="14">
        <location>
            <begin position="4"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="165"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="265"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="469"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="831"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="1077"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="439"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="528"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="670"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="791"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="891"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="933"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="558"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="710"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="799"/>
            <end position="824"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017313352.1"/>
        </source>
    </evidence>
    <sequence length="1138" mass="127002" checksum="096E62D97C85F99A" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q8F5</accession>
    <name>A0A2D0Q8F5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">arylamine N-acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC108259233</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the arylamine N-acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.5" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017314001.1">
        <property type="nucleotide sequence ID" value="XM_017458512.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q8F5"/>
    <dbReference type="GeneID" id="108259233"/>
    <dbReference type="KEGG" id="ipu:108259233"/>
    <dbReference type="OrthoDB" id="10260017at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004060">
        <property type="term" value="F:arylamine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2140.20:FF:000001">
        <property type="entry name" value="Arylamine N-acetyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2140.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001447">
        <property type="entry name" value="Arylamine_N-AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053710">
        <property type="entry name" value="Arylamine_NAT_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786:SF8">
        <property type="entry name" value="ARYLAMINE N-ACETYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786">
        <property type="entry name" value="N-HYDROXYARYLAMINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00797">
        <property type="entry name" value="Acetyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01543">
        <property type="entry name" value="ANATRNSFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017314001.1"/>
        </source>
    </evidence>
    <sequence length="304" mass="35051" checksum="B826C35AEADF00DA" modified="2017-12-20" version="1">MPDLLIGCISLENVMDLQEYFRRIGFGGTYEKPDLATLRTVHELHVMSVPFENLSIHCGERNTMDLQIIYEKIVRNRRGGWCCENNQLFSWVLREMGYKYTTLGSRVFNTLKNDYNPFNSHLINMVQIEGKPYIADVSFGVSCQIWHPLEMIAGKDQEQPAGIFCLLNDGETWTLQKTGRKPLVQNEAFANSSLIDKRVTKTMYSFLLIQREIDHFLNTSEYLQTSPESLFTQKSICSLQTPTGFRTLIGWTFSEVTFNPKEDTDIIEMRDVSDSEIETILKEKFNIVLVNKLTPINNKAAFKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QAL6</accession>
    <name>A0A2D0QAL6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ADP/ATP translocase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">ADP,ATP carrier protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">si:dkey-251i10.1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell. Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane.</text>
    </comment>
    <comment type="function">
        <text evidence="17">Catalyzes the exchange of ADP and ATP across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ADP(in) + ATP(out) = ADP(out) + ATP(in)</text>
            <dbReference type="Rhea" id="RHEA:34999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:35000"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>H(+)(in) = H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34979"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 17">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017315728.1">
        <property type="nucleotide sequence ID" value="XM_017460239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QAL6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030407"/>
    <dbReference type="GeneID" id="108260169"/>
    <dbReference type="KEGG" id="ipu:108260169"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005471">
        <property type="term" value="F:ATP:ADP antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140021">
        <property type="term" value="P:mitochondrial ADP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990544">
        <property type="term" value="P:mitochondrial ATP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901029">
        <property type="term" value="P:negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000002">
        <property type="entry name" value="Putative ADP/ATP translocase 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002113">
        <property type="entry name" value="ADT_euk_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635:SF14">
        <property type="entry name" value="ADP_ATP TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00927">
        <property type="entry name" value="ADPTRNSLCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0050">Antiport</keyword>
    <keyword evidence="11 15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="6"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="111"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="15">
        <location>
            <begin position="212"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU368008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017315728.1"/>
        </source>
    </evidence>
    <sequence length="298" mass="32911" checksum="B4A65477080B2F7F" modified="2017-12-20" version="1">MNETALSFAKDFLAGGIAAAISKTAVAPIERVKLLLQVQHASQQITVDKQYKGIMDCVVRIPKEQGFLSFWRGNLANVIRYFPTQALNFAFKDKYKKIFLDGVDKRKQFWRYFAGNLASGGAAGATSLCFVYPLDFARTRLAADVGKAGAGREFSGLGNCLVKVFKSDGLKGLYQGFNVSVQGIIIYRAAYFGIYDTAKGMMPDPKNTHIVVSWMIAQTVTAVAGLTSYPFDTVRRRMMMQSGRKGADIMYTGTIDCWKKIARDEGSKAFFKGAWSNVLRGMGGAFVLVLYDELKKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QKF3</accession>
    <name>A0A2D0QKF3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Histone H4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC108262037</name>
        <name evidence="18" type="synonym">zgc:153409</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18 19" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18 19">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 14">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the histone H4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017307302.1">
        <property type="nucleotide sequence ID" value="XM_017451813.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017318160.2">
        <property type="nucleotide sequence ID" value="XM_017462671.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017318211.1">
        <property type="nucleotide sequence ID" value="XM_017462722.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QKF3"/>
    <dbReference type="SMR" id="A0A2D0QKF3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000001961"/>
    <dbReference type="GeneID" id="108255684"/>
    <dbReference type="GeneID" id="108262037"/>
    <dbReference type="KEGG" id="ipu:108255684"/>
    <dbReference type="KEGG" id="ipu:108261934"/>
    <dbReference type="KEGG" id="ipu:108262037"/>
    <dbReference type="GeneTree" id="ENSGT00990000203553"/>
    <dbReference type="GeneTree" id="ENSGT01060000248528"/>
    <dbReference type="OrthoDB" id="9948295at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22912">
        <property type="entry name" value="HFD_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000002">
        <property type="entry name" value="Histone H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035425">
        <property type="entry name" value="CENP-T/H4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001951">
        <property type="entry name" value="Histone_H4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019809">
        <property type="entry name" value="Histone_H4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004823">
        <property type="entry name" value="TAF_TATA-bd_Histone-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10484">
        <property type="entry name" value="HISTONE H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15511">
        <property type="entry name" value="CENP-T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00623">
        <property type="entry name" value="HISTONEH4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00417">
        <property type="entry name" value="H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00803">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00047">
        <property type="entry name" value="HISTONE_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 14" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0164">Citrullination</keyword>
    <keyword evidence="10 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="12 14" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="11 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TATA box binding protein associated factor (TAF) histone-like fold" evidence="16">
        <location>
            <begin position="28"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017307302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017318211.1"/>
        </source>
    </evidence>
    <sequence length="103" mass="11367" checksum="A9E5DFD3F8B97598" modified="2017-12-20" version="1">MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QN81</accession>
    <name>A0A2D0QN81_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Prosaposin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">psap</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Prosaposin: Behaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling.</text>
    </comment>
    <comment type="function">
        <text evidence="7">Saposins are specific low-molecular mass non-enzymic proteins, they participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017319186.1">
        <property type="nucleotide sequence ID" value="XM_017463697.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QN81"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026300"/>
    <dbReference type="GeneID" id="108263172"/>
    <dbReference type="KEGG" id="ipu:108263172"/>
    <dbReference type="CTD" id="5660"/>
    <dbReference type="OMA" id="DICVHAG"/>
    <dbReference type="OrthoDB" id="69496at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007193">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019216">
        <property type="term" value="P:regulation of lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.225.10:FF:000002">
        <property type="entry name" value="prosaposin isoform X2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.225.10">
        <property type="entry name" value="Saposin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003119">
        <property type="entry name" value="SAP_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007856">
        <property type="entry name" value="SapB_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008138">
        <property type="entry name" value="SapB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008373">
        <property type="entry name" value="Saposin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011001">
        <property type="entry name" value="Saposin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021165">
        <property type="entry name" value="Saposin_chordata"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008139">
        <property type="entry name" value="SaposinB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051428">
        <property type="entry name" value="Sphingo_Act-Surfact_Prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480:SF95">
        <property type="entry name" value="PROSAPOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11480">
        <property type="entry name" value="SAPOSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02199">
        <property type="entry name" value="SapA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05184">
        <property type="entry name" value="SapB_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03489">
        <property type="entry name" value="SapB_2"/>
        <property type="match status" value="4"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QS02</accession>
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        <name type="scientific">Ictalurus punctatus</name>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
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                <person name="Kucuktas H."/>
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                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">SDA103</strain>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Liu S."/>
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                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
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                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
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                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
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                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
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        <subcellularLocation>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="F:adenylate cyclase activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0004383">
        <property type="term" value="F:guanylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001653">
        <property type="term" value="F:peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007168">
        <property type="term" value="P:receptor guanylyl cyclase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Guanylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011645">
        <property type="entry name" value="HNOB_dom_associated"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF497">
        <property type="entry name" value="GUANYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07701">
        <property type="entry name" value="HNOBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0141">cGMP biosynthesis</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="17">
        <location>
            <begin position="427"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="696"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="375"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012202"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023293"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017322589.1"/>
        </source>
    </evidence>
    <sequence length="717" mass="80443" checksum="E949D3D9644D1C4C" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QZQ0</accession>
    <name>A0A2D0QZQ0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peroxisomal multifunctional enzyme type 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">hsd17b4</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017467752.3"/>
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    <dbReference type="STRING" id="7998.ENSIPUP00000037537"/>
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    <dbReference type="KEGG" id="ipu:108265438"/>
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    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
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    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018812">
        <property type="term" value="F:3-hydroxyacyl-CoA dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048568">
        <property type="term" value="P:embryonic organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03448">
        <property type="entry name" value="HDE_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05353">
        <property type="entry name" value="hydroxyacyl-CoA-like_DH_SDR_c-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Hydroxysteroid 17-beta dehydrogenase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4290:FF:000001">
        <property type="entry name" value="Peroxisomal multifunctional enzyme type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000013">
        <property type="entry name" value="Peroxisomal multifunctional enzyme type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000185">
        <property type="entry name" value="peroxisomal multifunctional enzyme type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="entry name" value="SCP2 sterol-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054357">
        <property type="entry name" value="MFE-2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051687">
        <property type="entry name" value="Peroxisomal_Beta-Oxidation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45024">
        <property type="entry name" value="DEHYDROGENASES, SHORT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45024:SF2">
        <property type="entry name" value="SCP2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22622">
        <property type="entry name" value="MFE-2_hydrat-2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="12">
        <location>
            <begin position="11"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00073497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017323241.1"/>
        </source>
    </evidence>
    <sequence length="739" mass="79736" checksum="AEDFE5378CADD4EC" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R4D4</accession>
    <name>A0A2D0R4D4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">(S)-2-hydroxy-acid oxidase</fullName>
            <ecNumber evidence="2">1.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">hao2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2-hydroxyoctanoate + O2 = 2-oxooctanoate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:67940"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:133514"/>
            <dbReference type="ChEBI" id="CHEBI:176689"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:67941"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a (2S)-2-hydroxycarboxylate + O2 = a 2-oxocarboxylate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:16789"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:58123"/>
            <dbReference type="EC" id="1.1.3.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:16790"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.3.15" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017325467.1">
        <property type="nucleotide sequence ID" value="XM_017469978.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R4D4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005082"/>
    <dbReference type="GeneID" id="108266534"/>
    <dbReference type="KEGG" id="ipu:108266534"/>
    <dbReference type="CTD" id="51179"/>
    <dbReference type="OMA" id="WADFQYE"/>
    <dbReference type="OrthoDB" id="25826at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003973">
        <property type="term" value="F:(S)-2-hydroxy-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001561">
        <property type="term" value="P:fatty acid alpha-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02809">
        <property type="entry name" value="alpha_hydroxyacid_oxid_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000056">
        <property type="entry name" value="hydroxyacid oxidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012133">
        <property type="entry name" value="Alpha-hydoxy_acid_DH_FMN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008259">
        <property type="entry name" value="FMN_hydac_DH_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF149">
        <property type="entry name" value="2-HYDROXYACID OXIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000138">
        <property type="entry name" value="Al-hdrx_acd_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00557">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017325467.1"/>
        </source>
    </evidence>
    <sequence length="359" mass="40098" checksum="DC912241A0222338" modified="2017-12-20" version="1">MNIPAKVCLSDFEEYAKQHLSKPVWDYYSAGADECYTRDDNLQAYKRIRLRPRILRDVSVTDTQSTILGTEISFPVGIAPTAFHCLAWPEGELATAKATEVVNTCYITSTYSTCSVEEICAVAPNGYRWFQLYVYRDRKLSEQLVHRVEALGYKALVLTVDVPYTGKRRDDIRNQFKLPPHLKVKNFDGMFQDAAEPEEYGVPSCTLDPSISWKDIHWLQSLTRLPIIIKGILTKEDAELAVEHGVQGIIVSNHGGRQLDGGPATIDCLSEIVDTVQGRIEVYMDGGIRTGSDVLKALALGAKCVFIGRPAIWGLAYKGEDGVKEVLQILHEEFRLSMALSGCRNVAEINRNLIQVSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RB76</accession>
    <name>A0A2D0RB76_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peroxiredoxin-6</fullName>
            <ecNumber evidence="12">1.11.1.27</ecNumber>
            <ecNumber evidence="13">2.3.1.23</ecNumber>
            <ecNumber evidence="2">3.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">1-Cys peroxiredoxin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Acidic calcium-independent phospholipase A2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Glutathione-dependent peroxiredoxin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Lysophosphatidylcholine acyltransferase 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Non-selenium glutathione peroxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LOC108267850</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">SDA103</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41223"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:72999"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:41224"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>1-hexadecanoyl-sn-glycero-3-phosphocholine + hexadecanoyl-CoA = 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + CoA</text>
            <dbReference type="Rhea" id="RHEA:35983"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:72999"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:35984"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphocholine + CoA</text>
            <dbReference type="Rhea" id="RHEA:12937"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="EC" id="2.3.1.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a hydroperoxide + 2 glutathione = an alcohol + glutathione disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62632"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="EC" id="1.11.1.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peroxiredoxin family. Prx6 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.27" evidence="12"/>
    <dbReference type="EC" id="2.3.1.23" evidence="13"/>
    <dbReference type="EC" id="3.1.1.4" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017327798.1">
        <property type="nucleotide sequence ID" value="XM_017472309.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RB76"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019501"/>
    <dbReference type="GeneID" id="108267850"/>
    <dbReference type="KEGG" id="ipu:108267850"/>
    <dbReference type="OMA" id="HGPMNIP"/>
    <dbReference type="OrthoDB" id="2996783at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047184">
        <property type="term" value="F:1-acylglycerophosphocholine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03016">
        <property type="entry name" value="PRX_1cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1020.10:FF:000001">
        <property type="entry name" value="1-Cys peroxiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000011">
        <property type="entry name" value="Peroxiredoxin PRX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1020.10">
        <property type="entry name" value="Antioxidant, Horf6, Chain A, domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 21" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5 21" id="KW-0560">Oxidoreductase</keyword>
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    <keyword evidence="7 21" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="4"/>
            <end position="168"/>
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    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="22">
        <location>
            <position position="46"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
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            <dbReference type="ARBA" id="ARBA00023002"/>
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    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00024460"/>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00033065"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00048227"/>
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        <source>
            <dbReference type="PIRNR" id="PIRNR000239"/>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS51352"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_017327798.1"/>
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    <sequence length="223" mass="24886" checksum="C68783FF3E89B900" modified="2017-12-20" version="1">MPAPLLGDVFPNFEAETTIGKIRLHDYLGDSWGILFSHPRDYTPVCTTELGRAVALSPQFAKRGVKMIGLSIDSVEEHHGWSQDILAYNNDETVSRFPFPIIADGKRELAVELGMLDPNEKDQSGMPLTARCVFVIGPDKKLKLSLLYPATTGRNFNEILRVIDSLQLTAYQRVATPVDWKPGDRVMVPPSIPEEEAKTLFPAGVYTKELPSGKKYLRYTPQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RFX6</accession>
    <name>A0A2D0RFX6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Neuronal acetylcholine receptor subunit beta-3b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">chrnb3b</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017329439.1">
        <property type="nucleotide sequence ID" value="XM_017473950.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RFX6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027409"/>
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        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19064">
        <property type="entry name" value="LGIC_TM_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000005">
        <property type="entry name" value="Neuronal nicotinic acetylcholine receptor alpha4 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000001">
        <property type="entry name" value="Neuronal nicotinic acetylcholine receptor subunit 3"/>
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    </dbReference>
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        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Neur_chan_lig-bd"/>
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        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
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        <property type="entry name" value="Neur_channel"/>
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        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
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        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
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        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
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        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="12 22" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 18" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022251340" evidence="18">
        <location>
            <begin position="23"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="430"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="19">
        <location>
            <begin position="33"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="20">
        <location>
            <begin position="243"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="20">
        <location>
            <begin position="387"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000687"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02932"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017329439.1"/>
        </source>
    </evidence>
    <sequence length="464" mass="53470" checksum="2297C58C96AC816E" modified="2017-12-20" version="1">MKFARFVNAIFPLFLVAMVTAAGEFVSLAEREDTLLRDIFQGYQKWVRPTQHSNNTVKVRFGLKISQLVDVDEKNQLMTTNVWLWQEWVDYKLRWNPEDYGGITFIRVPTESIWLPDIVLYENADGHFEGSLMTKAIIKYNGAVTWTPPASYKSACTMDVTFFPFDRQNCSMKFGSWTYDGTMVDLVLVNHQVDRSDFFDNGEWEILSATGAKGSRHDGTLSYPFITYSFILKRLPLFYTLFLIIPCLGLSFLTVLVFYLPSDEGEKVTLSTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLLFIMIFVTLSIVVTVFVINVHHRSSATYHPMAPWVRDLFLQRLPQLLCMRTHTERYLYPELEPHSPDFKPRIRKARPGPEGEGQALVNMLEKATFSVRYISRHIRKENFIREVVHDWKFVAQVLDRIFLWVFLIVSILGTILIFTPALKMYLLSSSPPLAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RG31</accession>
    <name>A0A2D0RG31_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein Spindly</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Coiled-coil domain-containing protein 99</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Spindle apparatus coiled-coil domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">spdl1</name>
        <name evidence="8" type="synonym">CCDC99</name>
        <name evidence="8" type="synonym">SPDL1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Chromosome</location>
            <location evidence="8">Centromere</location>
            <location evidence="8">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Spindly family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017328855.1">
        <property type="nucleotide sequence ID" value="XM_017473366.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RG31"/>
    <dbReference type="GeneID" id="108268411"/>
    <dbReference type="KEGG" id="ipu:108268411"/>
    <dbReference type="CTD" id="54908"/>
    <dbReference type="OrthoDB" id="2121607at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000940">
        <property type="term" value="C:outer kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043515">
        <property type="term" value="F:kinetochore binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007080">
        <property type="term" value="P:mitotic metaphase chromosome alignment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03041">
        <property type="entry name" value="SPDLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028593">
        <property type="entry name" value="SPDLY_chordates"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051149">
        <property type="entry name" value="Spindly/BICDR_Dynein_Adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32123">
        <property type="entry name" value="BICD FAMILY-LIKE CARGO ADAPTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32123:SF9">
        <property type="entry name" value="PROTEIN SPINDLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 8" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 8" id="KW-0137">Centromere</keyword>
    <keyword evidence="1 8" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 8" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="3 8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="496"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="58"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="215"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="368"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="523"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017328855.1"/>
        </source>
    </evidence>
    <sequence length="632" mass="72150" checksum="7319FE19A8EBF982" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RHQ0</accession>
    <name>A0A2D0RHQ0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 15 16" type="primary">rab33ba</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14 15 16" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15 16">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017329672.1">
        <property type="nucleotide sequence ID" value="XM_017474183.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053538177.1">
        <property type="nucleotide sequence ID" value="XM_053682202.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053538178.1">
        <property type="nucleotide sequence ID" value="XM_053682203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RHQ0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026095"/>
    <dbReference type="GeneID" id="108268873"/>
    <dbReference type="KEGG" id="ipu:108268873"/>
    <dbReference type="CTD" id="572060"/>
    <dbReference type="GeneTree" id="ENSGT00940000157090"/>
    <dbReference type="OMA" id="PLWIEEC"/>
    <dbReference type="OrthoDB" id="10006973at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032482">
        <property type="term" value="P:Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04115">
        <property type="entry name" value="Rab33B_Rab33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000516">
        <property type="entry name" value="RAB33B, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041822">
        <property type="entry name" value="Rab33A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017329672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_053538177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_053538178.1"/>
        </source>
    </evidence>
    <sequence length="233" mass="26118" checksum="7BB5ACC1C8AFA220" modified="2017-12-20" version="1">MADMESSLEFSSSLTSSTLPPPRTRIFKIIVIGDSGVGKTCLTYRFCAGKFPDKTEATIGVDFREKLIEIDGEKIKVQLWDTAGQERFRKSMVQHYYRNVHAVVFVYDVTSAASFRSLPAWIEECRQHALGHEVPRILVGNKCDLRHAAQVSTELAQQFADAHSMPLFETSAKNPQGGTNDSDHVEAIFMTVAHKLKSQKPLVLSQPANTVTLRGQDQDEEQRGNWGCNCWRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RHX7</accession>
    <name>A0A2D0RHX7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Matrix metalloproteinase-14</fullName>
            <ecNumber evidence="6">3.4.24.80</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="33" type="primary">LOC108269081</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="32">SDA103</strain>
        </source>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="33">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endopeptidase activity. Activates progelatinase A by cleavage of the propeptide at 37-Asn-|-Leu-38. Other bonds hydrolyzed include 35-Gly-|-Ile-36 in the propeptide of collagenase 3, and 341-Asn-|-Phe-342, 441-Asp-|-Leu-442 and 354-Gln-|-Thr-355 in the aggrecan interglobular domain.</text>
            <dbReference type="EC" id="3.4.24.80"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="26">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="26">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="26">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="26">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Melanosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.80" evidence="6"/>
    <dbReference type="RefSeq" id="XP_017330129.1">
        <property type="nucleotide sequence ID" value="XM_017474640.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RHX7"/>
    <dbReference type="GeneID" id="108269081"/>
    <dbReference type="KEGG" id="ipu:108269081"/>
    <dbReference type="OMA" id="RKNDRPR"/>
    <dbReference type="OrthoDB" id="406838at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048870">
        <property type="term" value="P:cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000005">
        <property type="entry name" value="Matrix metallopeptidase 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.110.10.10:FF:000001">
        <property type="entry name" value="Matrix metallopeptidase 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.101.10:FF:000002">
        <property type="entry name" value="Matrix metalloproteinase-14 preproprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000585">
        <property type="entry name" value="Hemopexin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018486">
        <property type="entry name" value="Hemopexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021805">
        <property type="entry name" value="Pept_M10A_metallopeptidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021158">
        <property type="entry name" value="Pept_M10A_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF26">
        <property type="entry name" value="MATRIX METALLOPROTEINASE-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11857">
        <property type="entry name" value="DUF3377"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001191">
        <property type="entry name" value="Peptidase_M10A_matrix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00546">
        <property type="entry name" value="CYSTEINE_SWITCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00024">
        <property type="entry name" value="HEMOPEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51642">
        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18 26" id="KW-0106">Calcium</keyword>
    <keyword evidence="11" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="23" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="21 30" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 25" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20 33" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0645">Protease</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="12 30" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="19 30" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="17 25" id="KW-0862">Zinc</keyword>
    <keyword evidence="22" id="KW-0865">Zymogen</keyword>
    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="567"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="31">
        <location>
            <begin position="126"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="28">
        <location>
            <begin position="345"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="28">
        <location>
            <begin position="394"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="28">
        <location>
            <begin position="441"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="28">
        <location>
            <begin position="489"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="288"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="29">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="active site" evidence="24">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="26">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphotyrosine; by PKDCC" evidence="27">
        <location>
            <position position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="RefSeq" id="XP_017330129.1"/>
        </source>
    </evidence>
    <sequence length="610" mass="69811" checksum="46AB3FE887D8C46E" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RJY8</accession>
    <name>A0A2D0RJY8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SPARC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Osteonectin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Secreted protein acidic and rich in cysteine</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">sparc</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPARC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017330240.1">
        <property type="nucleotide sequence ID" value="XM_017474751.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RJY8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000001609"/>
    <dbReference type="GeneID" id="108269144"/>
    <dbReference type="KEGG" id="ipu:108269144"/>
    <dbReference type="CTD" id="6678"/>
    <dbReference type="OMA" id="CTDELMA"/>
    <dbReference type="OrthoDB" id="9972865at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005518">
        <property type="term" value="F:collagen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050840">
        <property type="term" value="F:extracellular matrix binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16235">
        <property type="entry name" value="EFh_SPARC_SPARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01328">
        <property type="entry name" value="FSL_SPARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000068">
        <property type="entry name" value="SPARC isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.30:FF:000004">
        <property type="entry name" value="SPARC isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003645">
        <property type="entry name" value="Fol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015369">
        <property type="entry name" value="Follistatin/Osteonectin_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001999">
        <property type="entry name" value="Osteonectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019577">
        <property type="entry name" value="SPARC/Testican_Ca-bd-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037641">
        <property type="entry name" value="SPARC_FS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13866:SF26">
        <property type="entry name" value="SPARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13866">
        <property type="entry name" value="SPARC OSTEONECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09289">
        <property type="entry name" value="FOLN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10591">
        <property type="entry name" value="SPARC_Ca_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00274">
        <property type="entry name" value="FOLN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00612">
        <property type="entry name" value="OSTEONECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00613">
        <property type="entry name" value="OSTEONECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0186">Copper</keyword>
    <keyword evidence="11 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012406753" description="SPARC" evidence="17">
        <location>
            <begin position="18"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="18">
        <location>
            <begin position="82"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="65"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="83"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="100"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="143"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="261"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR637641-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017330240.1"/>
        </source>
    </evidence>
    <sequence length="291" mass="33147" checksum="4E6BD30BF30EC9DC" modified="2017-12-20" version="1">MRVWIVFLLCLAGKALAAPAEEEPVIDEQVEVGSNPVQVETGEFDEAIEVVEDVAAENPCLNYHCKKGKVCELDDNNEPLCVCQDPLTCPAPVGEFEHVCGTDNKTYDSSCHFFATKCALEGTKKGHKLHLDYIGPCKYIAPCLENELGEFPLRMRDWLKNVMVTLYERDEDNNLLNEKQKLRVKKIFENEKRLQAGDHSLDLLVLDFEKNYNMYVFPVHWQFGQLDQHPSDGFLSHTELAPLRAPLIPMEHCTTSFFEACDADQDKYIALEEWANCFGIKEQDVDKDLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RMM5</accession>
    <name>A0A2D0RMM5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Metastasis-associated protein MTA1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">mta1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the metastasis-associated protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017331195.2">
        <property type="nucleotide sequence ID" value="XM_017475706.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RMM5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035293"/>
    <dbReference type="GeneID" id="108269729"/>
    <dbReference type="KEGG" id="ipu:108269729"/>
    <dbReference type="CTD" id="9112"/>
    <dbReference type="OrthoDB" id="2193595at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016581">
        <property type="term" value="C:NuRD complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04709">
        <property type="entry name" value="BAH_MTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11661">
        <property type="entry name" value="SANT_MTA3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000012">
        <property type="entry name" value="Metastasis-associated 1 family, member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.490:FF:000001">
        <property type="entry name" value="Metastasis-associated 1 family, member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.50:FF:000001">
        <property type="entry name" value="Metastasis-associated 1 family, member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000949">
        <property type="entry name" value="ELM2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040138">
        <property type="entry name" value="MIER/MTA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035170">
        <property type="entry name" value="MTA1_R1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10865">
        <property type="entry name" value="METASTASIS-ASSOCIATED PROTEIN AND MESODERM INDUCTION EARLY RESPONSE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10865:SF5">
        <property type="entry name" value="METASTASIS-ASSOCIATED PROTEIN MTA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01448">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17226">
        <property type="entry name" value="MTA_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01189">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51156">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BAH" evidence="13">
        <location>
            <begin position="4"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="ELM2" evidence="14">
        <location>
            <begin position="148"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="15">
        <location>
            <begin position="266"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="423"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="532"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="574"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="544"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00093454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017331195.2"/>
        </source>
    </evidence>
    <sequence length="616" mass="69495" checksum="22739C9541F7FA31" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RN13</accession>
    <name>A0A2D0RN13_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phospholipase DDHD1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ddhd1a</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PA-PLA1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017331546.1">
        <property type="nucleotide sequence ID" value="XM_017476057.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RN13"/>
    <dbReference type="GeneID" id="108269913"/>
    <dbReference type="KEGG" id="ipu:108269913"/>
    <dbReference type="CTD" id="562868"/>
    <dbReference type="OrthoDB" id="431378at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004177">
        <property type="entry name" value="DDHD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058055">
        <property type="entry name" value="PA-PLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057826">
        <property type="entry name" value="WWE_C20G8.02"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509">
        <property type="entry name" value="PA-PL1 PHOSPHOLIPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509:SF32">
        <property type="entry name" value="PHOSPHOLIPASE DDHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02862">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23463">
        <property type="entry name" value="WWE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01127">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51043">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDHD" evidence="4">
        <location>
            <begin position="588"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="79"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="674"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="742"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="553"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="690"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017331546.1"/>
        </source>
    </evidence>
    <sequence length="840" mass="95252" checksum="4947A5C9CDDCDEC2" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RP55</accession>
    <name>A0A2D0RP55_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CAAX prenyl protease</fullName>
            <ecNumber evidence="9">3.4.24.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">zmpste24</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Proteolytically removes the C-terminal three residues of farnesylated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 9">
            <text>Hydrolyzes the peptide bond -P2-(S-farnesyl or geranylgeranyl)C-P1'-P2'-P3'-COOH where P1' and P2' are amino acids with aliphatic side chains and P3' is any C-terminal residue.</text>
            <dbReference type="EC" id="3.4.24.84"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8 9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase M48A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.84" evidence="9"/>
    <dbReference type="RefSeq" id="XP_017332288.1">
        <property type="nucleotide sequence ID" value="XM_017476799.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RP55"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020930"/>
    <dbReference type="GeneID" id="108270277"/>
    <dbReference type="KEGG" id="ipu:108270277"/>
    <dbReference type="CTD" id="10269"/>
    <dbReference type="OMA" id="FVIEEKF"/>
    <dbReference type="OrthoDB" id="360839at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07343">
        <property type="entry name" value="M48A_Zmpste24p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027057">
        <property type="entry name" value="CAXX_Prtase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032456">
        <property type="entry name" value="Peptidase_M48_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10120">
        <property type="entry name" value="CAAX PRENYL PROTEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16491">
        <property type="entry name" value="Peptidase_M48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 9 14" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 8 9" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="119"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="382"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 1 N-terminal" evidence="12">
        <location>
            <begin position="36"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M48" evidence="11">
        <location>
            <begin position="224"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="288"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="330"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="413"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR627057-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR627057-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017332288.1"/>
        </source>
    </evidence>
    <sequence length="470" mass="53830" checksum="3998956A282B20C6" modified="2017-12-20" version="1">MLKEILDLPPEDKIFHAVLVFSWTVYLWEAYLSHRQRKIYSSATHVPTELGKIMDAETFEKSRLYQLDKSNFGFWSGLYSETEGTLILLFGGIPFLWKISGSLTGHFGLGPEYEISQSLVFLMLATLFSAIMGLPWSLYSTFVIEEKHGFNQQTLGFFLKDALKKFAVTQCILLPVTSLLLYIIKIGGDYFFIYAWLFTLVVSLVLVTIYADYIAPLFDKFTPLPEGELKTDIESMAKSIDFPLTKIYVVEGSKRSSHSNAYFYGFFKNKRIVLFDTLLEDYSPLNKKNDGESASEPDKNEETGDETKLQPKHKKQGCSNPEVLAVLGHELGHWKLGHTVKHIIISQVNSFLCFFLFAVLIGRQELFMAFGFHDNQPTLIGLLIIFQFIFSPYNELLTFCLTVLSRRFEFQADAFARAMGRSTELCSALIKLNKDNLGFPVADWLFSMWHYSHPPLLERLRAIKDTKKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RPC2</accession>
    <name>A0A2D0RPC2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14 15 16">Transcriptional-regulating factor 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14 15 16" type="primary">LOC108270139</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14 15 16" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15 16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017332011.1">
        <property type="nucleotide sequence ID" value="XM_017476522.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047013700.1">
        <property type="nucleotide sequence ID" value="XM_047157744.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047013701.1">
        <property type="nucleotide sequence ID" value="XM_047157745.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RPC2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005513"/>
    <dbReference type="GeneID" id="108270139"/>
    <dbReference type="KEGG" id="ipu:108270139"/>
    <dbReference type="GeneTree" id="ENSGT00940000160303"/>
    <dbReference type="OMA" id="TSINDMC"/>
    <dbReference type="OrthoDB" id="10258692at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
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    </dbReference>
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        <property type="entry name" value="TRANSCRIPTIONAL-REGULATING FACTOR 1"/>
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    </dbReference>
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        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
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    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="422"/>
            <end position="449"/>
        </location>
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        <location>
            <begin position="643"/>
            <end position="733"/>
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        <location>
            <begin position="748"/>
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        <location>
            <begin position="917"/>
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            <begin position="142"/>
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        <location>
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        </location>
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            <begin position="877"/>
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        </location>
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        </location>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
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        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
            <dbReference type="RefSeq" id="XP_047013700.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_047013701.1"/>
        </source>
    </evidence>
    <sequence length="986" mass="110037" checksum="689AC501797C3E16" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RQ38</accession>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC108270427</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">LOC108270427</name>
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        <name type="scientific">Ictalurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
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            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
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            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="549"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="647"/>
            <end position="668"/>
        </location>
    </feature>
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        <location>
            <begin position="104"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
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            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017332609.1"/>
        </source>
    </evidence>
    <sequence length="699" mass="77099" checksum="44D63214C1BD19AD" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RQN3</accession>
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        <submittedName>
            <fullName evidence="13">C-C chemokine receptor type 5</fullName>
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        <name evidence="13" type="primary">LOC108270521</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
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                <person name="Duke M.V."/>
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                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
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                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
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                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="nucleotide sequence ID" value="XM_017477315.2"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14984">
        <property type="entry name" value="7tmA_Chemokine_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000026">
        <property type="entry name" value="C-C chemokine receptor type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CCR1-9-like"/>
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    <dbReference type="InterPro" id="IPR000355">
        <property type="entry name" value="Chemokine_rcpt"/>
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    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF686">
        <property type="entry name" value="C-C CHEMOKINE RECEPTOR TYPE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00657">
        <property type="entry name" value="CCCHEMOKINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="251"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="62"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017332804.1"/>
        </source>
    </evidence>
    <sequence length="365" mass="41936" checksum="B63653F817D231FE" modified="2017-12-20" version="1">MAMTYSNNMTYSNSTPEDYSEYNLIQDSDSRFQLCNNNNVMTFSQVILPSLYSIVFIVGFIGNGLVLCVLVKYHNRSNVTDVCLFNLALSDLLFLISLPFWAHYAAISEWTFGGFMCHAVTALYMMGFYGSIFFMILMTVDRYAVIVRAHNSLFSKHRSVRAGIAVALFMWALSLGASLPTIIFSKVKNESDGSTCKVEYPQGTAWESFSYIKLNIVSLIFPLLVMVFCYSRIIPVLMAMKSQKKHKAVKLILVLIIIFFLFWTPYNIVIFLKFLHKLGYMNTCQWEQDRHMAMQWVETIAFSHCCLNPIIYAFVGQKFRNLVLKILKEWFPVCFGQCTTIVSEFSDRRSSIYSRSSEISTTKIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RRK6</accession>
    <name>A0A2D0RRK6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Tumor protein p53-inducible nuclear protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">tp53inp1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
            <location evidence="10">Nuclear body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017333134.1">
        <property type="nucleotide sequence ID" value="XM_017477645.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RRK6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030955"/>
    <dbReference type="GeneID" id="108270750"/>
    <dbReference type="KEGG" id="ipu:108270750"/>
    <dbReference type="CTD" id="94241"/>
    <dbReference type="OMA" id="VGQHIHC"/>
    <dbReference type="OrthoDB" id="10041339at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029431">
        <property type="entry name" value="TP53INP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31671">
        <property type="entry name" value="DIABETES AND OBESITY REGULATED, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31671:SF0">
        <property type="entry name" value="TUMOR PROTEIN P53-INDUCIBLE NUCLEAR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14839">
        <property type="entry name" value="DOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017333134.1"/>
        </source>
    </evidence>
    <sequence length="242" mass="26768" checksum="DD9B9C2E5C9AF6E5" modified="2017-12-20" version="1">MFQRFTSILFGDALEEASECPANVGFNRNEDDEDWILVDYLAEACTSACEDELIEVCSENIDAPDCPLRCSSCSSLDSAADTDPDDQGFLRLDAECGLEESWFITPPPCFTAGGRGPVLLETSPLENLLIEHPSMSVYAVHSSPHRQLKENSCEPCILRSEVQCRSSHPTGCYAAALTATRASFLEQAKNGRLAQRIRDNVQRQQLSRNALRRLNLLRDGGAKQAKSTAGHVHQPGQRQYNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RS99</accession>
    <name>A0A2D0RS99_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Vitellogenin 3, phosvitinless</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">vtg3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017333046.3">
        <property type="nucleotide sequence ID" value="XM_017477557.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RS99"/>
    <dbReference type="GeneID" id="108270710"/>
    <dbReference type="KEGG" id="ipu:108270710"/>
    <dbReference type="CTD" id="30518"/>
    <dbReference type="OrthoDB" id="5956066at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045735">
        <property type="term" value="F:nutrient reservoir activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071391">
        <property type="term" value="P:cellular response to estrogen stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032355">
        <property type="term" value="P:response to estradiol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.230.10">
        <property type="entry name" value="Lipovitellin, beta-sheet shell regions, chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.80.10">
        <property type="entry name" value="Lipovitellin-phosvitin complex, chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.50.20">
        <property type="entry name" value="Lipovitellin. Chain A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.90.10">
        <property type="entry name" value="Vitellinogen, beta-sheet shell domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.20">
        <property type="entry name" value="Vitellinogen, superhelical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015819">
        <property type="entry name" value="Lipid_transp_b-sht_shell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011030">
        <property type="entry name" value="Lipovitellin_superhlx_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015816">
        <property type="entry name" value="Vitellinogen_b-sht_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015258">
        <property type="entry name" value="Vitellinogen_b-sht_shell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037088">
        <property type="entry name" value="Vitellinogen_b-sht_shell_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015255">
        <property type="entry name" value="Vitellinogen_open_b-sht"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015817">
        <property type="entry name" value="Vitellinogen_open_b-sht_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050733">
        <property type="entry name" value="Vitellogenin/Apolipophorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001747">
        <property type="entry name" value="Vitellogenin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23345:SF29">
        <property type="entry name" value="VITELLOGENIN 3, PHOSVITINLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23345">
        <property type="entry name" value="VITELLOGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09175">
        <property type="entry name" value="Vit_b-sht_shell"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09172">
        <property type="entry name" value="Vit_open_b-sht"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01347">
        <property type="entry name" value="Vitellogenin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01169">
        <property type="entry name" value="DUF1943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01170">
        <property type="entry name" value="DUF1944"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00638">
        <property type="entry name" value="LPD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56968">
        <property type="entry name" value="Lipovitellin-phosvitin complex, beta-sheet shell regions"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48431">
        <property type="entry name" value="Lipovitellin-phosvitin complex, superhelical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51211">
        <property type="entry name" value="VITELLOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0758">Storage protein</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039921911" evidence="7">
        <location>
            <begin position="28"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="domain" description="Vitellogenin" evidence="8">
        <location>
            <begin position="36"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="174"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017333046.3"/>
        </source>
    </evidence>
    <sequence length="1242" mass="138695" checksum="16EF8F15A9EBA406" modified="2023-06-28" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RT93</accession>
    <name>A0A2D0RT93_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">E3 ubiquitin-protein ligase UHRF</fullName>
            <ecNumber evidence="19">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">RING-type E3 ubiquitin transferase UHRF</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Ubiquitin-like PHD and RING finger domain-containing protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Ubiquitin-like-containing PHD and RING finger domains protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">uhrf1</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">SDA103</strain>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Multi domain E3 ubiquitin ligase that also plays a role in DNA methylation and histone modifications.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 19">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18 19">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="19">The YDG domain mediates the interaction with histone H3.</text>
    </comment>
    <comment type="domain">
        <text evidence="19">The tudor-like regions specifically recognize and bind histone H3 unmethylated at 'Arg-2' (H3R2me0), while the PHD-type zinc finger specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3).</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="19"/>
    <dbReference type="RefSeq" id="XP_017333714.1">
        <property type="nucleotide sequence ID" value="XM_017478225.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RT93"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000037350"/>
    <dbReference type="GeneID" id="108271035"/>
    <dbReference type="KEGG" id="ipu:108271035"/>
    <dbReference type="CTD" id="29128"/>
    <dbReference type="OrthoDB" id="2270193at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044027">
        <property type="term" value="P:negative regulation of gene expression via chromosomal CpG island methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15616">
        <property type="entry name" value="PHD_UHRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16769">
        <property type="entry name" value="RING-HC_UHRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20455">
        <property type="entry name" value="Tudor_UHRF1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20457">
        <property type="entry name" value="Tudor_UHRF1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17122">
        <property type="entry name" value="Ubl_UHRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.280.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase UHRF1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000068">
        <property type="entry name" value="E3 ubiquitin-protein ligase UHRF1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000143">
        <property type="entry name" value="E3 ubiquitin-protein ligase UHRF1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.1150:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase UHRF2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000066">
        <property type="entry name" value="E3 ubiquitin-protein ligase UHRF2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.280.10">
        <property type="entry name" value="SRA-YDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036987">
        <property type="entry name" value="SRA-YDG_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003105">
        <property type="entry name" value="SRA_YDG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021991">
        <property type="entry name" value="TTD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047406">
        <property type="entry name" value="Ubl_UHRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045134">
        <property type="entry name" value="UHRF1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14140">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UHRF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14140:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UHRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02182">
        <property type="entry name" value="SAD_SRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12148">
        <property type="entry name" value="TTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00466">
        <property type="entry name" value="SRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51015">
        <property type="entry name" value="YDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="13 19" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15 18 19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 19" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10 19" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 17 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="22">
        <location>
            <begin position="6"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="21">
        <location>
            <begin position="316"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="23">
        <location>
            <begin position="324"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="YDG" evidence="24">
        <location>
            <begin position="425"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="23">
        <location>
            <begin position="716"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="89"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="288"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="625"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="288"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="625"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU369101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_017333714.1"/>
        </source>
    </evidence>
    <sequence length="785" mass="88512" checksum="27A6BA1476AF50E4" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RTW7</accession>
    <name>A0A2D0RTW7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription factor MafG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">V-maf musculoaponeurotic fibrosarcoma oncogene homolog G</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">mafgb</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family. Maf subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017333948.1">
        <property type="nucleotide sequence ID" value="XM_017478459.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RTW7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000031833"/>
    <dbReference type="GeneID" id="108271141"/>
    <dbReference type="KEGG" id="ipu:108271141"/>
    <dbReference type="CTD" id="405877"/>
    <dbReference type="OrthoDB" id="5974330at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045604">
        <property type="term" value="P:regulation of epidermal cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14717">
        <property type="entry name" value="bZIP_Maf_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000011">
        <property type="entry name" value="Transcription factor MafG, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004826">
        <property type="entry name" value="bZIP_Maf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008917">
        <property type="entry name" value="TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024874">
        <property type="entry name" value="Transcription_factor_Maf_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10129">
        <property type="entry name" value="TRANSCRIPTION FACTOR MAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10129:SF15">
        <property type="entry name" value="TRANSCRIPTION FACTOR MAFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03131">
        <property type="entry name" value="bZIP_Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47454">
        <property type="entry name" value="A DNA-binding domain in eukaryotic transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BZIP" evidence="12">
        <location>
            <begin position="84"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017333948.1"/>
        </source>
    </evidence>
    <sequence length="204" mass="22242" checksum="BEB55879C8B546F2" modified="2017-12-20" version="1">MGTPRCCRGVIPVPTSMKPIVKTSVWFTSGSRGMTTTNKGNKALKVKREPGENGTSLTDDELVSMSVRELNQHLRGLSKEEIVQLKQRRRTLKNRGYAASCRVKRVTQKEELERQKAELQQEVEKLASENASMKVELDALRSKYEALQSFARTVARAPAVPIANTPVPVSARGSLASVIGPLVPGKVGATSVITIVKSKTDARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RW25</accession>
    <name>A0A2D0RW25_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NEDD8 ultimate buster 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC108271341</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017334386.1">
        <property type="nucleotide sequence ID" value="XM_017478897.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RW25"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000036702"/>
    <dbReference type="GeneID" id="108271341"/>
    <dbReference type="KEGG" id="ipu:108271341"/>
    <dbReference type="OMA" id="SVCRLME"/>
    <dbReference type="OrthoDB" id="434245at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000058">
        <property type="term" value="P:regulation of ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14291">
        <property type="entry name" value="UBA1_NUB1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17062">
        <property type="entry name" value="Ubl_NUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039749">
        <property type="entry name" value="NUB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041207">
        <property type="entry name" value="NUB1_ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12948:SF3">
        <property type="entry name" value="NEDD8 ULTIMATE BUSTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12948">
        <property type="entry name" value="NEDD8 ULTIMATE BUSTER-1 BS4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18037">
        <property type="entry name" value="Ubiquitin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBA" evidence="2">
        <location>
            <begin position="359"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="2">
        <location>
            <begin position="407"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="452"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="504"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="463"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="504"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017334386.1"/>
        </source>
    </evidence>
    <sequence length="575" mass="65518" checksum="B2813892B993FAA4" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RYL5</accession>
    <name>A0A2D0RYL5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thymidylate kinase</fullName>
            <ecNumber evidence="3">2.7.4.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">dTMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">dtymk</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the phosphorylation of thymidine monophosphate (dTMP) to thymidine diphosphate (dTDP), the immediate precursor for the DNA building block dTTP, with ATP as the preferred phosphoryl donor in the presence of Mg(2+).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>dTMP + ATP = dTDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:13517"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58369"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thymidylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017335598.1">
        <property type="nucleotide sequence ID" value="XM_017480109.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RYL5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020208"/>
    <dbReference type="GeneID" id="108272028"/>
    <dbReference type="KEGG" id="ipu:108272028"/>
    <dbReference type="CTD" id="1841"/>
    <dbReference type="OMA" id="YWHQFDA"/>
    <dbReference type="OrthoDB" id="425602at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:dTMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006227">
        <property type="term" value="P:dUDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01672">
        <property type="entry name" value="TMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000679">
        <property type="entry name" value="Thymidylate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018095">
        <property type="entry name" value="Thymidylate_kin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344:SF1">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01331">
        <property type="entry name" value="THYMIDYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate kinase-like" evidence="13">
        <location>
            <begin position="11"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017335598.1"/>
        </source>
    </evidence>
    <sequence length="212" mass="24154" checksum="80E8E9B4F63E452F" modified="2017-12-20" version="1">MSCRRGALIVLEGVDKAGKTTQCKKLVRALQQSGRPAEMMRFPDRTTQIGQLISSYLEKKNNLEDHTVHLLFSANRWELVPLMKKKLEQGITLVVDRYAFSGVAFTSAKPDFSLEWCKHPDVGLPKPDLVMFLQLNPSVAAERGEFGTERYETSTFQRTVQQRFEQLMKDPSVNWKVIDAARSIDIVHSDIKVLCENTINMSENLPIGELWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RZ13</accession>
    <name>A0A2D0RZ13_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Protein Shroom2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">shroom2a</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the shroom family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017335200.1">
        <property type="nucleotide sequence ID" value="XM_017479711.3"/>
    </dbReference>
    <dbReference type="GeneID" id="108271852"/>
    <dbReference type="KEGG" id="ipu:108271852"/>
    <dbReference type="CTD" id="386817"/>
    <dbReference type="OrthoDB" id="10063560at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043296">
        <property type="term" value="C:apical junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06750">
        <property type="entry name" value="PDZ_shroom2_3_4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000100">
        <property type="entry name" value="Shroom family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3120">
        <property type="match status" value="1"/>
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S5K2</accession>
    <name>A0A2D0S5K2_ICTPU</name>
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        <recommendedName>
            <fullName evidence="3">Beta-galactoside alpha-2,6-sialyltransferase 2</fullName>
            <ecNumber evidence="17">2.4.3.1</ecNumber>
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        <alternativeName>
            <fullName evidence="14">CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 2</fullName>
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        <alternativeName>
            <fullName evidence="13">ST6Gal II</fullName>
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        <alternativeName>
            <fullName evidence="15">Sialyltransferase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20 21 22 23" type="primary">st6gal2b</name>
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    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20 21 22" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20 21 22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.</text>
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    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a beta-D-galactoside + CMP-N-acetyl-beta-neuraminate = an N-acetyl-alpha-neuraminyl-(2-&gt;6)-beta-D-galactosyl derivative + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52104"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28034"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:136398"/>
            <dbReference type="EC" id="2.4.3.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">Golgi stack membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017482512.3"/>
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        <property type="nucleotide sequence ID" value="XM_017482514.3"/>
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        <property type="nucleotide sequence ID" value="XM_017482515.3"/>
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        <property type="nucleotide sequence ID" value="XM_053684291.1"/>
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        <property type="component" value="Chromosome 12"/>
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    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:beta-galactoside alpha-2,6-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0097503">
        <property type="term" value="P:sialylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="ST6 beta-galactoside alpha-2,6-sialyltransferase 2"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Glyco_trans_29"/>
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        <property type="entry name" value="GT29-like_sf"/>
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    <dbReference type="PANTHER" id="PTHR46059">
        <property type="entry name" value="BETA-GALACTOSIDE ALPHA-2,6-SIALYLTRANSFERASE"/>
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        <property type="entry name" value="BETA-GALACTOSIDE ALPHA-2,6-SIALYLTRANSFERASE 2"/>
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        <property type="entry name" value="Glyco_transf_29"/>
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    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
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    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
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    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00060076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017338001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017338003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017338004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_053540266.1"/>
        </source>
    </evidence>
    <sequence length="410" mass="46903" checksum="9583DDD79ED9BE66" modified="2017-12-20" version="1">MKKKKHRKLFIVGVVTWVLLSFILFCQNTDSNLLVFCTFVLSPFTRGSMRLIDAPHVENSAGTEQLKEAILPTVMQQNLQLEESWADKVIRGLWVGLASSDMLSSYLQKAKKDYIAINLYRVTYTGHWTKQKDKKKLLCQLKQQKLLKTLTGSEEPFASLGWRKLVPQQPLEQAISGRYRTCAVVSSAGAILNSSLGREIDSHDAVLRFNAAPTEGYENDVGSKTTIRIINSQIMADPQHRFADSHLYKNVTLVAWDPGPYSGNLEAWYKNPDYDLFTAYVERRKRSPSQPFYILHPDFLWNLWDILQSNTEENVHPNPPSSGFIGIVLMMSICENLDIYEFIPSKRHTTLCHYYEKYHDLACTLGAYHPLFYEKLFIRRMTKVSLDELMTKGKVTLPGFSQITCSGQQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S7M8</accession>
    <name>A0A2D0S7M8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ornithine aminotransferase, mitochondrial</fullName>
            <ecNumber evidence="4">2.6.1.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Ornithine--oxo-acid aminotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">oat</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the reversible interconversion of L-ornithine and 2-oxoglutarate to L-glutamate semialdehyde and L-glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-ornithine + 2-oxoglutarate = L-glutamate 5-semialdehyde + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:25160"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="EC" id="2.6.1.13"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:25161"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="9">
            <dbReference type="Rhea" id="RHEA:25162"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-ornithine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.13" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017338436.1">
        <property type="nucleotide sequence ID" value="XM_017482947.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S7M8"/>
    <dbReference type="GeneID" id="108273605"/>
    <dbReference type="CTD" id="4942"/>
    <dbReference type="OrthoDB" id="425114at2759"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00358"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004587">
        <property type="term" value="F:ornithine aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019544">
        <property type="term" value="P:L-arginine catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010121">
        <property type="term" value="P:L-arginine catabolic process to proline via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000011">
        <property type="entry name" value="Ornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000152">
        <property type="entry name" value="Ornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010164">
        <property type="entry name" value="Orn_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01885">
        <property type="entry name" value="Orn_aminotrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF18">
        <property type="entry name" value="ORNITHINE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00069115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017338436.1"/>
        </source>
    </evidence>
    <sequence length="493" mass="54335" checksum="0C6AA3DEBA1ACC18" modified="2017-12-20" version="1">MKMNHYSVLHTVVLPHALTGFTTKATSLCLLGKFLVFRVVLHTLAEAQRIAMKCMLTHLTRWATPTLSRAAHSGMRSSSTVASCTKRDEVALSSEDIFSREERYGAHNYHPLPVALHRGEGVHVWDVEGRRYYDFLSAYSAVNQGHCHPKIVAALTGQASRLALTSRAFYNDVLGEYEEYITSLFGYSKVLPMNTGVEGGETACKLARKWAYCVKGVPQYQAKIVFAEGNFWGRTMAAISSSNDPSSYEGFGPFMPGFELVPYNDIPALEKAFQDPNVAAFMVEPIQGEAGVVVPDAGYLCKVRELCTKYNVLFIADEVQTGLARTGRRLAVDHEEVRPDLVVLGKALSGGMYPVSAVLCDDEVMLTIKPGEHGSTYGGNPLACRVAISALQVMEEEKLAENAAEMGKILRAELNKLPKEIVTKVRGKGLLNAIVIKETKDYNAWRVCLRLRDNGLLAKPTHGHIIRLAPPLIIKEDEIRECAEIIQGTVLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SCP5</accession>
    <name>A0A2D0SCP5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Alpha-mannosidase</fullName>
            <ecNumber evidence="17">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21 22" type="primary">man2a2</name>
    </gene>
    <organism evidence="20 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21 22" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21 22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + 2 H2O = 2 alpha-D-mannopyranose + an N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:56052"/>
            <dbReference type="Rhea" id="RHEA-COMP:14368"/>
            <dbReference type="Rhea" id="RHEA-COMP:14369"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28729"/>
            <dbReference type="ChEBI" id="CHEBI:60615"/>
            <dbReference type="ChEBI" id="CHEBI:60625"/>
            <dbReference type="EC" id="3.2.1.114"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="17">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="17"/>
    <dbReference type="RefSeq" id="XP_017340496.1">
        <property type="nucleotide sequence ID" value="XM_017485007.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017340497.1">
        <property type="nucleotide sequence ID" value="XM_017485008.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SCP5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005685"/>
    <dbReference type="GeneID" id="108274717"/>
    <dbReference type="KEGG" id="ipu:108274717"/>
    <dbReference type="CTD" id="4122"/>
    <dbReference type="OrthoDB" id="10261055at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004572">
        <property type="term" value="F:mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000001">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000009">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000002">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.110.10:FF:000003">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.50">
        <property type="entry name" value="Glycoside hydrolase family 38, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037094">
        <property type="entry name" value="Glyco_hydro_38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050843">
        <property type="entry name" value="Glycosyl_Hydrlase_38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607">
        <property type="entry name" value="ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607:SF57">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="Families 57/38 glycoside transferase middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 17" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 17" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="19">
        <location>
            <begin position="502"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017340496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017340497.1"/>
        </source>
    </evidence>
    <sequence length="1150" mass="130615" checksum="861B5115DB56DDD6" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SCU6</accession>
    <name>A0A2D0SCU6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein OSCP1a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">oscp1a</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017340070.1">
        <property type="nucleotide sequence ID" value="XM_017484581.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SCU6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008488"/>
    <dbReference type="GeneID" id="108274421"/>
    <dbReference type="KEGG" id="ipu:108274421"/>
    <dbReference type="CTD" id="100002307"/>
    <dbReference type="OMA" id="CGAKAEL"/>
    <dbReference type="OrthoDB" id="2157380at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019332">
        <property type="entry name" value="OSCP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21439">
        <property type="entry name" value="OXIDORED-NITRO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21439:SF0">
        <property type="entry name" value="PROTEIN OSCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10188">
        <property type="entry name" value="Oscp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="362"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="368"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017340070.1"/>
        </source>
    </evidence>
    <sequence length="385" mass="43438" checksum="5CA8C4072F15F00B" modified="2017-12-20" version="1">MSLRTLPLLIINLGGEMLYILDQRLRARNESDDKIQKGWSEDDRKRVMNDIVGVMFCKGFMEESMQPQDMYSHRALRYVLTRLAHTSIMRLNSTSMDRMYDLMTMAFKYQIALCPRPRDLLLVSFNHMDGVRELVKDNPRLVNLINEAQRLIIEMYTPLSDGEFQLIRQTLLAFFQDMRVRVSIFLKENLQNADGSFAIPTSGPVPHGSEVPGLIRYVNSRGRLVRRREFVSGGSYTSTVRDASFDVSGDRVTRLGTNMYGVSSAEDTHTSGSSKHTCKRQQVCDDPNPLAKAELNLLAKLMGQMEVWDSVGADGGMRVNLFPSDQEEDEEGRLSAADEACGVVSIQAVKADTELTRIAAQFTDQDEAERPSDKGEDLLAMMDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SDZ1</accession>
    <name>A0A2D0SDZ1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="31">Unconventional myosin-IXAb isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="31" type="primary">myo9ab</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30">SDA103</strain>
        </source>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="31">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="23">Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Regulates Rho by stimulating it's GTPase activity in neurons. Required for the regulation of neurite branching and motor neuron axon guidance.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 24">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017340470.1">
        <property type="nucleotide sequence ID" value="XM_017484981.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000019281"/>
    <dbReference type="GeneID" id="108274707"/>
    <dbReference type="KEGG" id="ipu:108274707"/>
    <dbReference type="CTD" id="562199"/>
    <dbReference type="OrthoDB" id="437889at2759"/>
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    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="RefSeq" id="XP_017340470.1"/>
        </source>
    </evidence>
    <sequence length="2465" mass="280745" checksum="4E699210E02CC1EA" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SHZ8</accession>
    <name>A0A2D0SHZ8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ddx19a</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017341905.1">
        <property type="nucleotide sequence ID" value="XM_017486416.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SHZ8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033934"/>
    <dbReference type="GeneID" id="108275535"/>
    <dbReference type="KEGG" id="ipu:108275535"/>
    <dbReference type="CTD" id="55308"/>
    <dbReference type="OMA" id="HTRKMAG"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000318">
        <property type="entry name" value="ATP-dependent RNA helicase DDX19B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000357">
        <property type="entry name" value="ATP-dependent RNA helicase DDX19B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 18" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="16">
        <location>
            <begin position="92"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="125"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="306"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="92"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017341905.1"/>
        </source>
    </evidence>
    <sequence length="479" mass="54108" checksum="192B6BCFC18E5AE2" modified="2017-12-20" version="1">MATDSWALSVDQQEATTKIFEVLRIRESRVVQNAELKETEKPTDGDKSDEEEKEDRAAQSLLNKLIRNSLVNNTNQVEVLQRDPNSPLYSVKTFEELHLKPQLLQGVYAMGFNRPSKIQETALPMMLAEPPQNLIAQSQSGTGKTAAFVLAMLSHVDPANKWTQCLCLSPTYELTLQTGKVVEQMGKFYPEVKLAYAIRGNKMDGGSKIQEQIVIGTPGTMSDWLTKKKVIDPKKVRVFVLDEADVMIGMQGHQDQSIRIQRKLPEDCQMLLFSATFEDTVWQFAERIVPDPNVIRLKREEETLDTIKQYYVLCKDRQDKFNALCNIYGAITIAQAMIFCHTRRTAAWLSAKMAADGHQVALLSGELAVEQRAAIIERFREGKEKVLITTNVCARGIDVEQVSVVINFDLPLDKDGNPDNETYLHRIGRTGRFGKRGLAINMVDSRRSLAVLRAIEEHFNKKIVKLDTGDLDEMEKIAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SNU7</accession>
    <name>A0A2D0SNU7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">E3 ubiquitin-protein ligase TRIM23</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">ADP-ribosylation factor domain-containing protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">GTP-binding protein ARD-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">RING-type E3 ubiquitin transferase TRIM23</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Tripartite motif-containing protein 23</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="primary">trim23</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="29">SDA103</strain>
        </source>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="30">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Acts as an E3 ubiquitin-protein ligase. Plays an essential role in autophagy activation during viral infection. Mechanistically, activates TANK-binding kinase 1/TBK1 by facilitating its dimerization and ability to phosphorylate the selective autophagy receptor SQSTM1. In order to achieve this function, TRIM23 mediates 'Lys-27'-linked auto-ubiquitination of its ADP-ribosylation factor (ARF) domain to induce its GTPase activity and its recruitment to autophagosomes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">Homodimer. Interacts with PSCD1. Interacts with UBE2D2. Interacts with TBK1 (via N-terminal kinase domain) and p62/SQSTM1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">In the C-terminal section; belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="RefSeq" id="XP_053542928.1">
        <property type="nucleotide sequence ID" value="XM_053686953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SNU7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029252"/>
    <dbReference type="GeneID" id="108276764"/>
    <dbReference type="KEGG" id="ipu:108276764"/>
    <dbReference type="CTD" id="373"/>
    <dbReference type="OMA" id="IQACDAK"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04158">
        <property type="entry name" value="ARD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19773">
        <property type="entry name" value="Bbox2_TRIM23_C-IX_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19774">
        <property type="entry name" value="Bbox2_TRIM23_C-IX_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16645">
        <property type="entry name" value="mRING-HC-C3HC3D_TRIM23_C-IX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000440">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000130">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000486">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003649">
        <property type="entry name" value="Bbox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00502">
        <property type="entry name" value="BBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="16 27" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="18" id="KW-0458">Lysosome</keyword>
    <keyword evidence="28" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 28" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 27" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11" id="KW-0863">Zinc-finger</keyword>
    <feature type="binding site" evidence="27">
        <location>
            <begin position="416"/>
            <end position="423"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="423"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <begin position="518"/>
            <end position="521"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00057906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00061142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00062428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00072862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00075959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00076802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00079668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00083908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="RefSeq" id="XP_053542928.1"/>
        </source>
    </evidence>
    <sequence length="579" mass="64733" checksum="3D4F7B5C135D81FB" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SQ11</accession>
    <name>A0A2D0SQ11_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Sorting nexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">snx18b</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017344370.1">
        <property type="nucleotide sequence ID" value="XM_017488881.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SQ11"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032401"/>
    <dbReference type="GeneID" id="108276851"/>
    <dbReference type="KEGG" id="ipu:108276851"/>
    <dbReference type="CTD" id="548603"/>
    <dbReference type="OMA" id="GNGEAYH"/>
    <dbReference type="OrthoDB" id="10254720at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036089">
        <property type="term" value="P:cleavage furrow formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000033">
        <property type="entry name" value="Sorting nexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000116">
        <property type="entry name" value="Sorting nexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000004">
        <property type="entry name" value="Sorting nexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014536">
        <property type="entry name" value="Snx9_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019497">
        <property type="entry name" value="Sorting_nexin_WASP-bd-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45827">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45827:SF5">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10456">
        <property type="entry name" value="BAR_3_WASP_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027744">
        <property type="entry name" value="Snx9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="11 12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="17">
        <location>
            <begin position="216"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="89"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR027744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR027744-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017344370.1"/>
        </source>
    </evidence>
    <sequence length="557" mass="62901" checksum="C67A3F931682EC75" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SQG3</accession>
    <name>A0A2D0SQG3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="24">Solute carrier family 12 member 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">slc12a2</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>K(+)(out) + 2 chloride(out) + Na(+)(out) = K(+)(in) + 2 chloride(in) + Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72395"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:72396"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC12A transporter family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017344937.1">
        <property type="nucleotide sequence ID" value="XM_017489448.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A2D0SQG3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000004597"/>
    <dbReference type="GeneID" id="108277086"/>
    <dbReference type="KEGG" id="ipu:108277086"/>
    <dbReference type="CTD" id="6558"/>
    <dbReference type="OrthoDB" id="2020542at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008511">
        <property type="term" value="F:sodium:potassium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006884">
        <property type="term" value="P:cell volume homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055064">
        <property type="term" value="P:chloride ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055075">
        <property type="term" value="P:potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055078">
        <property type="term" value="P:sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000005">
        <property type="entry name" value="Solute carrier family 12 member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013612">
        <property type="entry name" value="AA_permease_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002444">
        <property type="entry name" value="NKCC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018491">
        <property type="entry name" value="SLC12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002443">
        <property type="entry name" value="SLC12A1/SLC12A2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004842">
        <property type="entry name" value="SLC12A_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00930">
        <property type="entry name" value="2a30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827:SF58">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12 MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08403">
        <property type="entry name" value="AA_permease_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03522">
        <property type="entry name" value="SLC12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01207">
        <property type="entry name" value="NAKCLTRNSPRT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01208">
        <property type="entry name" value="NAKCLTRSPRT1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0868">Chloride</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0915">Sodium</keyword>
    <keyword evidence="15" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="249"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="365"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="417"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="451"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="588"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="614"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="644"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease N-terminal" evidence="22">
        <location>
            <begin position="133"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="20">
        <location>
            <begin position="221"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="21">
        <location>
            <begin position="734"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="882"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="72"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF03522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF08403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_017344937.1"/>
        </source>
    </evidence>
    <sequence length="1149" mass="125751" checksum="32E36389CDBC9217" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SQJ1</accession>
    <name>A0A2D0SQJ1_ICTPU</name>
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        <submittedName>
            <fullName evidence="12 13">PDZ and LIM domain protein 5b isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">pdlim5b</name>
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    <organism evidence="11 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12 13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017344765.1">
        <property type="nucleotide sequence ID" value="XM_017489276.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017344766.1">
        <property type="nucleotide sequence ID" value="XM_017489277.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SQJ1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006815"/>
    <dbReference type="GeneID" id="108277016"/>
    <dbReference type="KEGG" id="ipu:108277016"/>
    <dbReference type="CTD" id="393165"/>
    <dbReference type="GeneTree" id="ENSGT00940000155292"/>
    <dbReference type="OMA" id="TISSXAS"/>
    <dbReference type="OrthoDB" id="5911912at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031941">
        <property type="term" value="C:filamentous actin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051371">
        <property type="term" value="F:muscle alpha-actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="LIM1_Enigma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09362">
        <property type="entry name" value="LIM2_Enigma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06753">
        <property type="entry name" value="PDZ_PDLIM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000019">
        <property type="entry name" value="LIM domain binding 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000010">
        <property type="entry name" value="PDZ and LIM domain protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000014">
        <property type="entry name" value="PDZ and LIM domain protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000020">
        <property type="entry name" value="PDZ and LIM domain protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031847">
        <property type="entry name" value="PDLI1-4/Zasp-like_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050604">
        <property type="entry name" value="PDZ-LIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214:SF32">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15936">
        <property type="entry name" value="DUF4749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6 7" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="453"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="512"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="572"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="85"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="267"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="378"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="143"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="171"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="181"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="204"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="280"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="378"/>
            <end position="400"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017344765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017344766.1"/>
        </source>
    </evidence>
    <sequence length="631" mass="67502" checksum="CF6EE7EF4B5ABEFD" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SWH7</accession>
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        <recommendedName>
            <fullName evidence="3 8">Chordin</fullName>
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    </protein>
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        <name evidence="10" type="primary">chrd</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the chordin family.</text>
    </comment>
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        <property type="component" value="Unplaced"/>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="FnI-like domain"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50933">
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        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR002496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017347036.1"/>
        </source>
    </evidence>
    <sequence length="955" mass="106444" checksum="FBAFCDC3E44BD6A7" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SXV2</accession>
    <name>A0A2D0SXV2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC108278465</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108278465</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017347351.1">
        <property type="nucleotide sequence ID" value="XM_017491862.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SXV2"/>
    <dbReference type="GeneID" id="108278465"/>
    <dbReference type="KEGG" id="ipu:108278465"/>
    <dbReference type="OrthoDB" id="8963759at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017347351.1"/>
        </source>
    </evidence>
    <sequence length="209" mass="22784" checksum="B8AD3262A7423BFC" modified="2017-12-20" version="1">MPTESVTEATAIQFPTQSVESSDGLISQHIEQVPSAEGPPSDSCKTAVSQACWEVNVAFMLVVLAGFLILALFYCVLHLWHKLRLAQAGNALEYFGFYHMANYSLKHQHEPTSLPSVNTDPPVQVPPCYPPQTVVPENSHPVIPPLLPPPPLLSSRPPSAVPLPHPIIYTTPPSPQSDAEVYSRIGTLRPARHSSVSQTQVVLFEHSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SYV1</accession>
    <name>A0A2D0SYV1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">WD repeat-containing protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">wdr5</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8 9">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat WDR5/wds family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347847.1">
        <property type="nucleotide sequence ID" value="XM_017492358.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053543721.1">
        <property type="nucleotide sequence ID" value="XM_053687746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SYV1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018479"/>
    <dbReference type="GeneID" id="108278780"/>
    <dbReference type="KEGG" id="ipu:108278780"/>
    <dbReference type="CTD" id="11091"/>
    <dbReference type="GeneTree" id="ENSGT00940000154143"/>
    <dbReference type="OMA" id="CKGHDTA"/>
    <dbReference type="OrthoDB" id="674604at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000029">
        <property type="entry name" value="WD repeat-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059122">
        <property type="entry name" value="Beta-prop_WDR5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25175">
        <property type="entry name" value="Beta-prop_WDR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002394">
        <property type="entry name" value="GN-bd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDR5-like beta-propeller" evidence="6">
        <location>
            <begin position="40"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="41"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="83"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="125"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="167"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="210"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="252"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="13"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017347847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_053543721.1"/>
        </source>
    </evidence>
    <sequence length="334" mass="36446" checksum="F119DB457D49F946" modified="2017-12-20" version="1">MASEEKKPETEATKTPTASAASASQSKSAPAKPNYTLKFTLAGHTKAVSSVKFSPSGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVSSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNMVYIWNLQTKEIVQKLQGHTDVVISTACHPTENIIASAALENDKTIKLWKSDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T2D9</accession>
    <name>A0A2D0T2D9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">POU domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">pou5f3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the POU transcription factor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017348976.1">
        <property type="nucleotide sequence ID" value="XM_017493487.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T2D9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012889"/>
    <dbReference type="GeneID" id="108279325"/>
    <dbReference type="KEGG" id="ipu:108279325"/>
    <dbReference type="CTD" id="30333"/>
    <dbReference type="OMA" id="SFWPGIS"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000161">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000022">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF128">
        <property type="entry name" value="POU DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00157">
        <property type="entry name" value="Pou"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00028">
        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00352">
        <property type="entry name" value="POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00035">
        <property type="entry name" value="POU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00465">
        <property type="entry name" value="POU_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="344"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="188"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="412"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="214"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="246"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017348976.1"/>
        </source>
    </evidence>
    <sequence length="473" mass="51467" checksum="F1EE642ADE7BC3B9" modified="2017-12-20" version="1">MSDRPESPPEDCGNRAYELSRPMSSYSSALGHEGLQFPHSMLQDPGLLFNKAPFNGLNAAHTQTFFSFPSVGGDFRHNDAQVGDFSQPKHWYPFTAPEYTGQVAGATAATQPANLSPPIAETREQIKILEVKTEKDGSEEYDGAETKLQPQFLGPATSVPAGVYYSSSAPWTPSFWPGLSHIAAPTTVAQAPPTPSASSPSLSPSPPGNGFGSPGFYSGAAAQNAAATQVQNSARSSGSSSGGCSDSEEEENLTTEDLEQFAKELKHKRITLGFTQADVGLALGNLYGKMFSQTTICRFEALQLSFKNMCKLKPLLQKWLNEAETSENPQDMYKVERVFVDTRKRKRRTSLEGSVRTALESYFLKCPKPNTLEITHISDDLGLERDVVRVWFCNRRQKGKRLALPFDEDGAEGQYYDQSQTPPPHMGGTPLSGQGYPGPGHASGMPTLYMPPLHRSEVFKQALHPGLVGHLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T882</accession>
    <name>A0A2D0T882_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Glial cell line-derived neurotrophic factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC108280527</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TGF-beta family. GDNF subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017351089.1">
        <property type="nucleotide sequence ID" value="XM_017495600.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T882"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020719"/>
    <dbReference type="GeneID" id="108280527"/>
    <dbReference type="KEGG" id="ipu:108280527"/>
    <dbReference type="OMA" id="YSTWHAL"/>
    <dbReference type="OrthoDB" id="9936891at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030116">
        <property type="term" value="F:glial cell-derived neurotrophic factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043401">
        <property type="entry name" value="GDNF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12173">
        <property type="entry name" value="GDNF SUBFAMILY OF TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12173:SF8">
        <property type="entry name" value="PERSEPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0339">Growth factor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="TGF-beta family profile" evidence="9">
        <location>
            <begin position="137"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="113"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="113"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017351089.1"/>
        </source>
    </evidence>
    <sequence length="259" mass="29125" checksum="BB4C98F5836360E4" modified="2017-12-20" version="1">MSSGVNRRLTGGRRWRRGRAKRGLFAMLSAATDATFQRRNWKMVVWVLVSLLPLVVGDRPGFGEDPVDAVGLVTTMLDEQPFDALPEEDEEMGDGDSYSPWHNVFEPSVLVEEEHPARWERSPRSSDASDAQSKGSRKKKKKKKKEKEKGEDEGRGQSSRDCHLERREMRVRDLGMGYDSDEIVLFKFCVGLCQSARGNYDAALRALLTNGSLPKRTARKVSARPCCRPTAYKSVSFMDTSTTWRTIENVSALDCKCVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0TA25</accession>
    <name>A0A2D0TA25_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein THEM6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">them6</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the THEM6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017351723.1">
        <property type="nucleotide sequence ID" value="XM_017496234.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0TA25"/>
    <dbReference type="GeneID" id="108280811"/>
    <dbReference type="KEGG" id="ipu:108280811"/>
    <dbReference type="CTD" id="51337"/>
    <dbReference type="OMA" id="RDIDMCH"/>
    <dbReference type="OrthoDB" id="265761at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="CDD" id="cd00586">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051490">
        <property type="entry name" value="THEM6_lcsJ_thioesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12475">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12475:SF4">
        <property type="entry name" value="PROTEIN THEM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13279">
        <property type="entry name" value="4HBT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00041112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017351723.1"/>
        </source>
    </evidence>
    <sequence length="204" mass="23322" checksum="CD185F0C4F0F66C2" modified="2017-12-20" version="1">MGDLLLWVLAVLLVLFCFFDVWYYLRVLAVLLRAWFLSPVFDVTAEQAVGGHVLLNDIDMCHMNNARYLRECDFARISLCARNGVLKATRALGAAMVAGAITVRYRRPLCVGEKFELRTRIVTWDEKAFYIEQRFVSSKDGMVAAVLFCKLNVMRGSPDKILQHLCKRKVEVPEFPEELQHWISFITASSQALKAESDLEKKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EDH3</accession>
    <name>A0A979EDH3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">Uncharacterized protein LOC108255357 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">LOC108255357</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4 5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047005891.1">
        <property type="nucleotide sequence ID" value="XM_047149935.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053530596.1">
        <property type="nucleotide sequence ID" value="XM_053674621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EDH3"/>
    <dbReference type="GeneID" id="108255357"/>
    <dbReference type="KEGG" id="ipu:108255357"/>
    <dbReference type="OrthoDB" id="8977941at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="167"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_047005891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053530596.1"/>
        </source>
    </evidence>
    <sequence length="225" mass="24859" checksum="06D485336B4504E4" modified="2023-02-22" version="1">MLKADYSLRGLYMAKCVSETVCEVVFTLSPLEMTHVITPGEPIDVYLPLTDMVEVIFTASGAVQPSHPQICTVDKEKIQCSPDYKERASLLNTLQIKDGDVSDSGNYTVRDKVNDETLAIVTVHMRGSEPEKPSSGAAPVSQTRIRRRRSVSSEENPCPEFKQERSMPAWGITLIALMSLVMAVMMGVIVYQQKEIQQLQKNPADTELTLTQTNGDANPNPGEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EDI4</accession>
    <name>A0A979EDI4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BRI3-binding protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bri3bp</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047005922.1">
        <property type="nucleotide sequence ID" value="XM_047149966.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EDI4"/>
    <dbReference type="GeneID" id="108255483"/>
    <dbReference type="CTD" id="140707"/>
    <dbReference type="OrthoDB" id="9889463at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033367">
        <property type="entry name" value="BRI3BP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31253">
        <property type="entry name" value="BRI3-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31253:SF0">
        <property type="entry name" value="BRI3-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14965">
        <property type="entry name" value="BRI3BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5036718835" evidence="2">
        <location>
            <begin position="21"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_047005922.1"/>
        </source>
    </evidence>
    <sequence length="245" mass="26797" checksum="DC8899CA834DC288" modified="2023-02-22" version="1">MKTVTLALVLCCLLMAEAEAARSRKEWSGGSSGGGGGLKRAASGVYHSLSSVFGEDNIKALYKFFSRTNERFVHGVDSLLDTLWRSCTDLLDALGLESSTVSHYFSPSAMSSSPSRAVLLLGAVFLVYWFVSLVLGGVMLVLRAVLGRFFWAARVLFFALSCLYVLQKFEGDPEKAVLPLLVIMAVYFMTGPVSAYWRRQGVSTLEDKIDNLETQEYAGCVFIGIHMKCFCGPASHHLSLQCSTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EFU5</accession>
    <name>A0A979EFU5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">GTPase IMAP family member 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC108258417</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047007372.2">
        <property type="nucleotide sequence ID" value="XM_047151416.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EFU5"/>
    <dbReference type="GeneID" id="108258417"/>
    <dbReference type="KEGG" id="ipu:108258417"/>
    <dbReference type="OrthoDB" id="5985928at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000366">
        <property type="entry name" value="GTPase, IMAP family member 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF188">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
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        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_047007372.2"/>
        </source>
    </evidence>
    <sequence length="767" mass="86863" checksum="43A8A4A67F32596A" modified="2023-06-28" version="2"> </sequence>
</entry>
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            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
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        </lineage>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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        <source>
            <dbReference type="PROSITE" id="PS50181"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
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        <source>
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    <sequence length="653" mass="75138" checksum="4A01D741768AEE9E" modified="2023-02-22" version="1"> </sequence>
</entry>
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                <person name="Jiang C."/>
                <person name="Sun L."/>
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                <person name="Zhou T."/>
                <person name="Zeng Q."/>
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                <person name="Koren S."/>
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                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
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                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
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    <dbReference type="RefSeq" id="XP_047006543.1">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:transmembrane signaling receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF87">
        <property type="entry name" value="CMRF35-LIKE MOLECULE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
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    </dbReference>
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        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="20"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="7"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
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            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_047006543.1"/>
        </source>
    </evidence>
    <sequence length="322" mass="36300" checksum="095AFCEB139FDEFF" modified="2023-02-22" version="1">MAVLLLPVLILQAISGCACLWTVEKVTAKPGGSITIPCHYNWRFREQEKYWCRGRMWYMCVRVREWAGRVSVMDNPDKLVTTLMFSELQVRDAGRYWCAVKNGGLMSDIRTSMELQVTDDPDLWVSRALLSAPEGGIVHVQCLYSDRLKKVEKKWCRGGDLHSCQSQHSAQISQNAQLQLSDEGQGLFSVARAGLRTDEAGWYWCSAAGVQAPVHICITTTHNYTLQNDTVLEYPSSTPESPPIATPTTSHRWSVCVIVLLAAVMSVLVGAVCWRWRTHQPEVQVRERWTESAELSVTDDAELLDRDWSRAAVLQLQEESNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F209</accession>
    <name>A0A979F209_ICTPU</name>
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        <recommendedName>
            <fullName evidence="13">Striatin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">strn</name>
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            <taxon>Actinopterygii</taxon>
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        </lineage>
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    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="12">Part of the core of STRIPAK complexes composed of PP2A catalytic and scaffolding subunits, the striatins (PP2A regulatory subunits), the striatin-associated proteins MOB4, STRIP1 and STRIP2, PDCD10 and members of the STE20 kinases, such as STK24 and STK26. Interacts with CTTNBP2; this interaction may regulate dendritic spine distribution of STRN. Activation of glutamate receptors weakens the interaction with CTTNBP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat striatin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047013949.1">
        <property type="nucleotide sequence ID" value="XM_047157993.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979F209"/>
    <dbReference type="GeneID" id="108271087"/>
    <dbReference type="CTD" id="6801"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070016">
        <property type="term" value="F:armadillo repeat domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051721">
        <property type="term" value="F:protein phosphatase 2A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.300:FF:000001">
        <property type="entry name" value="striatin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000058">
        <property type="entry name" value="striatin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000079">
        <property type="entry name" value="striatin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000211">
        <property type="entry name" value="striatin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013258">
        <property type="entry name" value="Striatin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051488">
        <property type="entry name" value="WD_repeat_striatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15653">
        <property type="entry name" value="STRIATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15653:SF2">
        <property type="entry name" value="STRIATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08232">
        <property type="entry name" value="Striatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0770">Synapse</keyword>
    <keyword evidence="6 14" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Striatin N-terminal" evidence="17">
        <location>
            <begin position="43"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="431"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="484"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="537"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="674"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="189"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="263"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="61"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="204"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="270"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00063979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_047013949.1"/>
        </source>
    </evidence>
    <sequence length="752" mass="82593" checksum="15206FA50A78114A" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979FF01</accession>
    <name>A0A979FF01_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Tropomyosin alpha-3 chain isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC108280389</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the tropomyosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047018591.1">
        <property type="nucleotide sequence ID" value="XM_047162635.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979FF01"/>
    <dbReference type="GeneID" id="108280389"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000001">
        <property type="entry name" value="Tropomyosin alpha-1 chain isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000001">
        <property type="entry name" value="Tropomyosin alpha-1 chain isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000533">
        <property type="entry name" value="Tropomyosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19269">
        <property type="entry name" value="TROPOMYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00261">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00194">
        <property type="entry name" value="TROPOMYOSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00326">
        <property type="entry name" value="TROPOMYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="7"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_047018591.1"/>
        </source>
    </evidence>
    <sequence length="251" mass="29395" checksum="0EAE1D8C3E0985CA" modified="2023-02-22" version="1">MAGVNSVEAVKRKIKVLQQQADEAEERAEVLQRQVEEEKRSREQAEADVASLNRRIQLVEEELDRAQERLTTALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEVQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAEVAEANRRRLEELLRNYDQTLKSLQAAQDKYSQKEDKYEEEIKILTDKLKEAETRAEFGERSVTKLEKTIDDLEDERTETEREAITEELDRALNDMTSIEARHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QTA5</accession>
    <name>A0A9F7QTA5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Distal membrane-arm assembly complex protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP synthase subunit s-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">dmac2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Required for the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). Involved in the assembly of the distal region of complex I.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with incompletely assembled mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP synthase subunit s family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053529480.1">
        <property type="nucleotide sequence ID" value="XM_053673505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QTA5"/>
    <dbReference type="GeneID" id="108280671"/>
    <dbReference type="KEGG" id="ipu:108280671"/>
    <dbReference type="CTD" id="55101"/>
    <dbReference type="OrthoDB" id="1708588at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000168">
        <property type="entry name" value="Distal membrane arm assembly complex 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF169">
        <property type="entry name" value="F-BOX AND LEUCINE-RICH REPEAT PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00057777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00062608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00072316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00076566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053529480.1"/>
        </source>
    </evidence>
    <sequence length="257" mass="29333" checksum="DE73FEBFD9F969B8" modified="2023-06-28" version="1">MAASMLFQMRRHCSLVPCLTVAKRQYASNPPSLFSKVFLQLNQRFLEVEYLIRLGNWFKNRDVRRKNLFYDYAQKKYGVNVAAAYSILKLGGAFRFAGQTEWFCTDSKGKFSFDFVNTPDSTIEEIDLSRTLINQAGLHNLMSQRKLRSLSVQGCPEVDDWFLARLHVFSETLQELNLSHCPRITVGGLAALQHLRQLQQLDISSLPQVQSPGLVHILVEEMLPHCKVIGAEYDQGLQIQTDNREPTDADSLRHAQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QUV1</accession>
    <name>A0A9F7QUV1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Dystrobrevin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19 20 21 22 23 24" type="primary">dtnba</name>
    </gene>
    <organism evidence="18 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19 20 21" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19 20 21">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the dystrophin family. Dystrobrevin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017310830.2">
        <property type="nucleotide sequence ID" value="XM_017455341.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017310842.2">
        <property type="nucleotide sequence ID" value="XM_017455353.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053533383.1">
        <property type="nucleotide sequence ID" value="XM_053677408.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053533390.1">
        <property type="nucleotide sequence ID" value="XM_053677415.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053533397.1">
        <property type="nucleotide sequence ID" value="XM_053677422.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053533405.1">
        <property type="nucleotide sequence ID" value="XM_053677430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QUV1"/>
    <dbReference type="GeneID" id="108257508"/>
    <dbReference type="KEGG" id="ipu:108257508"/>
    <dbReference type="CTD" id="325242"/>
    <dbReference type="OrthoDB" id="6019271at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099536">
        <property type="term" value="P:synaptic signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16244">
        <property type="entry name" value="EFh_DTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02334">
        <property type="entry name" value="ZZ_dystrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000014">
        <property type="entry name" value="Dystrobrevin alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000016">
        <property type="entry name" value="Dystrobrevin alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000002">
        <property type="entry name" value="Dystrobrevin alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017432">
        <property type="entry name" value="Distrobrevin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015153">
        <property type="entry name" value="EF-hand_dom_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015154">
        <property type="entry name" value="EF-hand_dom_typ2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050774">
        <property type="entry name" value="KCMF1/Dystrophin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268:SF22">
        <property type="entry name" value="DYSTROBREVIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12268">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE KCMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09068">
        <property type="entry name" value="EF-hand_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09069">
        <property type="entry name" value="EF-hand_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038204">
        <property type="entry name" value="Distrobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0770">Synapse</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="17">
        <location>
            <begin position="238"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="516"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="606"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="545"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="617"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR038204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017310830.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017310842.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_053533383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_053533390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_053533397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_053533405.1"/>
        </source>
    </evidence>
    <sequence length="654" mass="73332" checksum="2464ACFE46F1FA81" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R5J2</accession>
    <name>A0A9F7R5J2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein LOC108255699</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC108255699</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the intercrine alpha (chemokine CxC) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053530503.1">
        <property type="nucleotide sequence ID" value="XM_053674528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R5J2"/>
    <dbReference type="GeneID" id="108255699"/>
    <dbReference type="KEGG" id="ipu:108255699"/>
    <dbReference type="OrthoDB" id="9948647at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00273">
        <property type="entry name" value="Chemokine_CXC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039809">
        <property type="entry name" value="Chemokine_b/g/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001089">
        <property type="entry name" value="Chemokine_CXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033899">
        <property type="entry name" value="CXC_Chemokine_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015:SF191">
        <property type="entry name" value="C-X-C MOTIF CHEMOKINE 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015">
        <property type="entry name" value="SMALL INDUCIBLE CYTOKINE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00436">
        <property type="entry name" value="INTERLEUKIN8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00437">
        <property type="entry name" value="SMALLCYTKCXC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00199">
        <property type="entry name" value="SCY"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="4" id="KW-0202">Cytokine</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039911979" evidence="8">
        <location>
            <begin position="21"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="9">
        <location>
            <begin position="23"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="9">
        <location>
            <begin position="99"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="9">
        <location>
            <begin position="175"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053530503.1"/>
        </source>
    </evidence>
    <sequence length="244" mass="26553" checksum="1A71124C0AAA4870" modified="2023-06-28" version="1">MKSAAVFVVFACLLIVHVQGQAKMRCLCQGPAANVVHPLRIDKIDIHPASTSCENKEIVVTLKNGGGKKCLNPESEFTKKYITARGIIPHVQGQARTSVRRCLCQGPAANVVRPQRIDKIEIHPASATCENVEIIVTLKNGGGKKCLNPESEFTKKYITARGIIPHVQGQARTSVRRCLCQGPAANVVRPQRIDKIEIHPASATCENVEIIVTLKNGAGKKCLNPESEFTKKYITAGLEKRSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R893</accession>
    <name>A0A9F7R893_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Translation initiation factor eIF2B subunit delta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">eIF2B GDP-GTP exchange factor subunit delta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">eif2b4</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit. Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053531881.1">
        <property type="nucleotide sequence ID" value="XM_053675906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R893"/>
    <dbReference type="GeneID" id="100304826"/>
    <dbReference type="CTD" id="8890"/>
    <dbReference type="OrthoDB" id="10254737at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10470:FF:000002">
        <property type="entry name" value="Probable translation initiation factor eIF-2B subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10233">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10233:SF14">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 13" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="22"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="115"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_053531881.1"/>
        </source>
    </evidence>
    <sequence length="547" mass="60662" checksum="B523222BAC5A5B72" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R894</accession>
    <name>A0A9F7R894_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">LOW QUALITY PROTEIN: son of sevenless homolog 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC108263000</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053535312.1">
        <property type="nucleotide sequence ID" value="XM_053679337.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108263000"/>
    <dbReference type="KEGG" id="ipu:108263000"/>
    <dbReference type="OrthoDB" id="546434at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22914">
        <property type="entry name" value="HFD_SOS1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22915">
        <property type="entry name" value="HFD_SOS1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000029">
        <property type="entry name" value="son of sevenless homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000015">
        <property type="entry name" value="son of sevenless homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF150">
        <property type="entry name" value="SON OF SEVENLESS HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="6">
        <location>
            <begin position="196"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="439"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="7">
        <location>
            <begin position="589"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="5">
        <location>
            <begin position="775"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1016"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1042"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1070"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1136"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1241"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1074"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1146"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1195"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1247"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1318"/>
            <end position="1330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_053535312.1"/>
        </source>
    </evidence>
    <sequence length="1339" mass="153532" checksum="85682AE01CC77E1C" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R962</accession>
    <name>A0A9F7R962_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Interferon-induced protein 44-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108261227</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053532391.1">
        <property type="nucleotide sequence ID" value="XM_053676416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R962"/>
    <dbReference type="GeneID" id="108261227"/>
    <dbReference type="KEGG" id="ipu:108261227"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14241">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14241:SF1">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="1">
        <location>
            <begin position="27"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053532391.1"/>
        </source>
    </evidence>
    <sequence length="122" mass="13490" checksum="5D5BD0CD12FE0ACE" modified="2023-06-28" version="1">MPWGCKCFLEEKLRNFKLCSSDVKFVRILVVGEVGAGKSSFINAVNNAFQEQITSGTLVDGRSGTSFTKIYKTHHIKGKDGSRLPFVFSDVMGLESADEQGAHVKDIISAFSLRGFLRKDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RB29</accession>
    <name>A0A9F7RB29_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Homeobox protein Meis1a isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">meis1a</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TALE/MEIS homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053532092.1">
        <property type="nucleotide sequence ID" value="XM_053676117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RB29"/>
    <dbReference type="GeneID" id="108258533"/>
    <dbReference type="CTD" id="170455"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000004">
        <property type="entry name" value="Meis2 homeobox isoform 2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032453">
        <property type="entry name" value="PKNOX/Meis_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16493">
        <property type="entry name" value="Meis_PKNOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="7">
        <location>
            <begin position="206"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="208"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="125"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_053532092.1"/>
        </source>
    </evidence>
    <sequence length="321" mass="35584" checksum="BEE5ECE35336B36A" modified="2023-06-28" version="1">MGSAVNDALKRDKDAIYGHPLFPLLALVFEKCELATCTPRESGLAADVCSSASFDEDITVFSKQIRAEKPFFTPSPDLDNLMVQAIQVLRFHLLELEKVHELCDNFCHRYINCLKGKMPMDLVVDDREGSSKSDSEDFSRNSGGTADQMCWREDDSVSVHSTEGPAPCGGRSSSLNGDNSSEHGDLLDHGAASPSTGDDDETDKDRRNNKKRGIFPKVATNIMRAWLFQHLTHPYPSEEQKKQLAQDTGLTILQVNNWFINARRRIVQPMIDQSNRAVGHSGPYTPDSQPMGGYMLDGQAHMAARASGSLGMGMDSQWHYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RCW0</accession>
    <name>A0A9F7RCW0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC108260344</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_053532913.1">
        <property type="nucleotide sequence ID" value="XM_053676938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RCW0"/>
    <dbReference type="GeneID" id="108260344"/>
    <dbReference type="KEGG" id="ipu:108260344"/>
    <dbReference type="OrthoDB" id="1022360at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01093">
        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000011">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000069">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000001">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF11">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PAK 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="12">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="311"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="189"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="260"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_053532913.1"/>
        </source>
    </evidence>
    <sequence length="587" mass="65693" checksum="3B17294E7B8B0935" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RE17</accession>
    <name>A0A9F7RE17_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Involucrin-like isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC128634207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053540441.1">
        <property type="nucleotide sequence ID" value="XM_053684466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RE17"/>
    <dbReference type="GeneID" id="128634207"/>
    <dbReference type="KEGG" id="ipu:128634207"/>
    <dbReference type="OrthoDB" id="8963348at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053540441.1"/>
        </source>
    </evidence>
    <sequence length="361" mass="43245" checksum="83DCDDA5C1909117" modified="2023-06-28" version="1">METRQTLSKLTGLSSPDTPETYDYRPVKEMGTATGNNPSQTIEDTTKQTCKIKKNKKEKKEKHLHMKVNYEKKEKNLEAREIAVEKRETHQDRKEIHLEEREKHLNEKETNLAEKEKHLQQKEITLQEKGKHLDGKEVQFEEKEKHLDGKEMNLAEKEKHLQQRGIHLQEKEKHLDGKEVQFEEKEKQLFVREINLGKRSKYLEGRETRLQERTKHLEEKDISLEGSLKHLLGRENSLQERTKHLEEKEVSLDERLKHLLGRENSLQERGKCLDGREVQLEKREMRIDRRESRVDEREQRLEGKEIHLEEKEKNLAQHDLRHLNSADRENEKQERSSFMKSSKKQKREHPDEEEKEHSVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RF88</accession>
    <name>A0A9F7RF88_ICTPU</name>
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        <submittedName>
            <fullName evidence="13">Retinoic acid receptor alpha-B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC128629556</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Heterodimer; with an rxr molecule. Binds DNA preferentially as a rar/rxr heterodimer.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_053678153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RF88"/>
    <dbReference type="GeneID" id="128629556"/>
    <dbReference type="KEGG" id="ipu:128629556"/>
    <dbReference type="OrthoDB" id="6081310at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035259">
        <property type="term" value="F:nuclear glucocorticoid receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071376">
        <property type="term" value="P:cellular response to corticotropin-releasing hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048384">
        <property type="term" value="P:retinoic acid receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06937">
        <property type="entry name" value="NR_LBD_RAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000073">
        <property type="entry name" value="Retinoic acid receptor beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047158">
        <property type="entry name" value="NR_LBD_RAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003078">
        <property type="entry name" value="Retinoic_acid_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24085">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24085:SF8">
        <property type="entry name" value="RETINOIC ACID RECEPTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01292">
        <property type="entry name" value="RETNOICACIDR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="NR LBD" evidence="11">
        <location>
            <begin position="42"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="275"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="286"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011431"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_053534128.1"/>
        </source>
    </evidence>
    <sequence length="308" mass="34356" checksum="FC4F7ED14810634E" modified="2023-06-28" version="1">KSLPAGLIRLCSSFAAVRNDRNKKKKEEKKPECSESYVMSPDTEQMIERVRKAHQDTFPSLCQLGKYTTNNSADHRVSLDVDLWDKFSELSTKCIIKTVEFAKQLPGFTTLTIADQITLLKAACLDILILRICTRYTPDQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFANQLLPLEMDDAETGLLSAICLLCGDRQDLEESDKVDILQEPLLEALKIYVRRRRPHKPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGSMPPLIQEMLENSEGLEGSSMNPPGSCSPSLSPSSIHSSPSTHSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RH02</accession>
    <name>A0A9F7RH02_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Protocadherin beta-16-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC128635185</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053542769.1">
        <property type="nucleotide sequence ID" value="XM_053686794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RH02"/>
    <dbReference type="GeneID" id="128635185"/>
    <dbReference type="KEGG" id="ipu:128635185"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF296">
        <property type="entry name" value="PROTOCADHERIN 1 GAMMA 11 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="696"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="86"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="143"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="252"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="357"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="462"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="586"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_053542769.1"/>
        </source>
    </evidence>
    <sequence length="828" mass="91835" checksum="493EC00C472B275A" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RHG5</accession>
    <name>A0A9F7RHG5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC128631794</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC128631794</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053535313.1">
        <property type="nucleotide sequence ID" value="XM_053679338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RHG5"/>
    <dbReference type="GeneID" id="128631794"/>
    <dbReference type="KEGG" id="ipu:128631794"/>
    <dbReference type="OrthoDB" id="8828665at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042849">
        <property type="entry name" value="ARHGEF33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46944">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46944:SF1">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053535313.1"/>
        </source>
    </evidence>
    <sequence length="403" mass="44447" checksum="20649172BA7A9A92" modified="2023-06-28" version="1">MMKMMMMMLQEEGEKFRLLSLLLAPVTRLHTYLNTVQVLLSCSSSEHSDWRSLHNSNQVLRNLYTRCHMSLERAGRWTDGAGRPKEGAESVNGLSSRCCAESPECARSAFTKYRANEHQRDPHHLTVPRPLLTPAGILRNSRERLCEPPPPAGWTESCMWDFRAVECRPVPARRPQNLFLNPGAPGELPASIPTLKSPSGLAERLCGSPGPDGRVCRSDAYALPQRIDDADTDADVGDASVFDYSSVTTCSPDDTLELRGRDEDEEDDEEEDSEIPVLLKPSYTHAAGGLQREGSVCSRWQIPRAAPLPPEVCVAGSGTGRRTTKHRRKIPTGAFRPIWDAPFKQGEGAPAKENTVSFSSTNQIINPQRPSDSSRSVYRSVCGGERDGLWNDSDDSEGPCSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RLY7</accession>
    <name>A0A9F7RLY7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kelch-like protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">klhl3</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the KLHL3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053537972.1">
        <property type="nucleotide sequence ID" value="XM_053681997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RLY7"/>
    <dbReference type="GeneID" id="108268847"/>
    <dbReference type="CTD" id="26249"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18513">
        <property type="entry name" value="BACK_KLHL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000002">
        <property type="entry name" value="Kelch-like family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000001">
        <property type="entry name" value="Kelch-like family member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030578">
        <property type="entry name" value="KLHL3_BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF179">
        <property type="entry name" value="KELCH-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00501">
        <property type="entry name" value="KELCHREPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="12">
        <location>
            <begin position="51"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_053537972.1"/>
        </source>
    </evidence>
    <sequence length="588" mass="65520" checksum="1E97B25F524E5877" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RS02</accession>
    <name>A0A9F7RS02_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC108261631 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108261631</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053542666.1">
        <property type="nucleotide sequence ID" value="XM_053686691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RS02"/>
    <dbReference type="GeneID" id="108261631"/>
    <dbReference type="KEGG" id="ipu:108261631"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="420"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053542666.1"/>
        </source>
    </evidence>
    <sequence length="441" mass="46585" checksum="FC6E6B3C2634F946" modified="2023-06-28" version="1">MTIWCCSAVSSTRRYIQRALSCCAQRKTFSRKQQFARMDDWGVRMPPTLVEYFATICQEAEEYQAVLQKQQDARNYLAFNSPPMFMKDYAMPHQQEGESAVRANTISHPPSPIMDLIQPARSAKDVAPPSCSAEDIAPSSCSVEVVAPPSCSVEVVAPPSCLAEVVAPPSCSAEVVALPSCSAEDVALPSCSAEVVAPPSCSAEVIAPPSCFEEDVALPPCSTEDVIPLFCCVPYVTPPSCSTEDIVPLSCSTENLAPLPGLAGNLAPLYGLAEIFALPSGLAENFAPLSCPAEVVVPLSCHADFALSSRPAGGVAPFFCSAEEAAQSPGSWDILPRGSGPPDGGGACLGAPGVVPWGGFCHVSGNSELQFPSATALHVHDHLHLIHVSVNEHSCVYIATVCTVYLSFVVLDFRVPCHCVLFHAIVVCLVVLVSLVCCVFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TBX4</accession>
    <name>A0A9F7TBX4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">E3 ubiquitin-protein ligase TRIM35</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC128629027</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053531074.1">
        <property type="nucleotide sequence ID" value="XM_053675099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TBX4"/>
    <dbReference type="GeneID" id="128629027"/>
    <dbReference type="KEGG" id="ipu:128629027"/>
    <dbReference type="OrthoDB" id="6105938at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12893">
        <property type="entry name" value="SPRY_PRY_TRIM35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="12"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="84"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="8">
        <location>
            <begin position="272"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="194"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053531074.1"/>
        </source>
    </evidence>
    <sequence length="471" mass="54147" checksum="2B5E1FC42C26D77F" modified="2023-06-28" version="1">MASKFSEEEFSCPVCCEIFTDPVLLRCSHSVCKVCLQKFWENKGSRECPVCRRKSSMEDPPICLALKNLCETFLQERSPRSSSASETVCSLHREKLKLFCLDDQQPVCWVCRDSKIHTNHKFCPIDEALTDCKEELKTALKPLQEKLKIFKDCKLHWSQTTEHIKIQAQHTERQIKEEFEKLHQVLRDEEAARIAALREEEEQKSQMMKEKIEKLSRDISSLSDTIRGVEEEMRAEDVSFLQNYEATVRRAQCTLQHPEELSGTLIHVAKHLDNLKFRVWEKMQDAVQYTPVTLDPNTADPLLIVSDDLTSVRFSDEEQKLPDNPERFDDYSYCILGSEVFNSGTHCWDVEVGDCTLWLVGVMTESAQRKGNIFSRSGIWCVGYCGGKYGTCSTTQSFTLLSVTQNLQRIRVQLDWNRGKLSFSDPLTNTHIHTFTHTFTDKLLPFINVGCNKSPVKILPLQCSVRVNQIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TCP2</accession>
    <name>A0A9F7TCP2_ICTPU</name>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein LOC108254731 isoform X3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC108254731</name>
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    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_053673918.1"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="8938325at2759"/>
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        <property type="component" value="Chromosome 21"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <end position="169"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053529893.1"/>
        </source>
    </evidence>
    <sequence length="262" mass="27832" checksum="76D8655E90946380" modified="2023-06-28" version="1">MSDKHNMMHFFTTTSTTTTTTTTTQTSTPEEKPSADLSLVTTPPSTATEEPTAASPVFSTPLITTRHTATPESTRVTQNHHHNTTSAPNNLSSPPTPSSTTKEKTDSALSTVSTSQTTPKDKSTASPVSISIVNPTSGPKSRSLPTVLSVTGVCVLLAGLMGFCLCVCINKQKTESRCKLNTTHRDQGVMMSVLNMNNSGSEAAETYSVISSVPLPALPLDTSGDIKVDSKITVVDTYHVYSSIPDTHVTTNDAVYSLLQMH</sequence>
</entry>
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    <name>A0A9F7THW2_ICTPU</name>
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        <submittedName>
            <fullName evidence="8">Basement membrane-specific heparan sulfate proteoglycan core protein</fullName>
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        <name evidence="8" type="primary">LOC108267272</name>
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    <organism evidence="7 8">
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        <name type="common">Channel catfish</name>
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        <lineage>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
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                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
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    </reference>
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    <dbReference type="KEGG" id="ipu:108267272"/>
    <dbReference type="OrthoDB" id="6151406at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
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    <dbReference type="GO" id="GO:0009897">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="leukocyte immune-type receptor 2 isoform X2"/>
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        <property type="entry name" value="Leukocyte immune-type receptor TS32.15 L2.5a"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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    <dbReference type="InterPro" id="IPR050488">
        <property type="entry name" value="Ig_Fc_receptor"/>
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        <property type="entry name" value="Ig_sub"/>
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        <property type="entry name" value="Ig_sub2"/>
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        <property type="entry name" value="Ig_V-set"/>
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    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
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    <dbReference type="PANTHER" id="PTHR11481:SF64">
        <property type="entry name" value="FC RECEPTOR-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481">
        <property type="entry name" value="IMMUNOGLOBULIN FC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="8"/>
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        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="IG"/>
        <property type="match status" value="15"/>
    </dbReference>
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        <property type="entry name" value="IGc2"/>
        <property type="match status" value="9"/>
    </dbReference>
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        <property type="entry name" value="IGv"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
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    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
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        <location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
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    <name>A0A9F7TI18_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">KAT8 regulatory NSL complex subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">NSL complex protein NSL3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Non-specific lethal 3 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">kansl3</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle pole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053536073.1">
        <property type="nucleotide sequence ID" value="XM_053680098.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TI18"/>
    <dbReference type="GeneID" id="108265516"/>
    <dbReference type="CTD" id="55683"/>
    <dbReference type="OrthoDB" id="6415022at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044545">
        <property type="term" value="C:NSL complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000032">
        <property type="entry name" value="KAT8 regulatory NSL complex subunit 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046879">
        <property type="entry name" value="KANL3/Tex30_Abhydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056519">
        <property type="entry name" value="KANSL3_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026555">
        <property type="entry name" value="NSL3/Tex30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13136:SF16">
        <property type="entry name" value="KAT8 REGULATORY NSL COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13136">
        <property type="entry name" value="TESTIS DEVELOPMENT PROTEIN PRTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20408">
        <property type="entry name" value="Abhydrolase_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23154">
        <property type="entry name" value="KANSL3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KANSL3 helical" evidence="15">
        <location>
            <begin position="135"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="KANL3/Tex30 alpha/beta hydrolase-like" evidence="14">
        <location>
            <begin position="327"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="478"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="483"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="519"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="530"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00077273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00079519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF20408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_053536073.1"/>
        </source>
    </evidence>
    <sequence length="869" mass="92774" checksum="B2EB2AB51A8D5EEE" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TIK4</accession>
    <name>A0A9F7TIK4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Protein piccolo-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC128629582</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053534289.1">
        <property type="nucleotide sequence ID" value="XM_053678314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TIK4"/>
    <dbReference type="GeneID" id="128629582"/>
    <dbReference type="KEGG" id="ipu:128629582"/>
    <dbReference type="OrthoDB" id="10059918at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048788">
        <property type="term" value="C:cytoskeleton of presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098982">
        <property type="term" value="C:GABA-ergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098882">
        <property type="term" value="F:structural constituent of presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904071">
        <property type="term" value="P:presynaptic active zone assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035418">
        <property type="term" value="P:protein localization to synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052098">
        <property type="entry name" value="Presynaptic_Scaffold_Bsn/Pclo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008899">
        <property type="entry name" value="Znf_piccolo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14113">
        <property type="entry name" value="PICCOLO/BASSOON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14113:SF6">
        <property type="entry name" value="PROTEIN PICCOLO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05715">
        <property type="entry name" value="zf-piccolo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger piccolo-type" evidence="6">
        <location>
            <begin position="152"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="26"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053534289.1"/>
        </source>
    </evidence>
    <sequence length="195" mass="20496" checksum="B588A8E768EEBD6B" modified="2023-06-28" version="1">MFSSNFLGGGNPLSAMSSAVNKFSLFGDETEEGKEKQQQGPLKQKIPEQHGNGQPVSQGSQKHSTEKPPGGRMDDPQQQGIGRGLAQHPLSGRGSPQQQRAGRGGPHHQGVGRGKSPQRETGRGGPLQQDPTGPGTHQVEITAASAAGMESLCPICKNTKFNFQSKEPPNHNTCTQCKSVVCSMCGFSPPDAAVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TKB5</accession>
    <name>A0A9F7TKB5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mucin-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC128632783</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053535966.1">
        <property type="nucleotide sequence ID" value="XM_053679991.1"/>
    </dbReference>
    <dbReference type="GeneID" id="128632783"/>
    <dbReference type="KEGG" id="ipu:128632783"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="910"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1171"/>
            <end position="1555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1568"/>
            <end position="1816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1568"/>
            <end position="1775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1782"/>
            <end position="1793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1801"/>
            <end position="1816"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053535966.1"/>
        </source>
    </evidence>
    <sequence length="1816" mass="187103" checksum="B78A3F9286BD3EEC" modified="2023-06-28" version="1"> </sequence>
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    <accession>A0A9F7TN04</accession>
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        <submittedName>
            <fullName evidence="4">Kinesin-associated protein 3b isoform X1</fullName>
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        <name evidence="4" type="primary">kifap3b</name>
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            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
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            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053543194.1">
        <property type="nucleotide sequence ID" value="XM_053687219.1"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="10265679at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016939">
        <property type="term" value="C:kinesin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
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        <property type="entry name" value="ARM-type_fold"/>
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        <property type="entry name" value="KAP3"/>
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    <dbReference type="PANTHER" id="PTHR15605:SF2">
        <property type="entry name" value="KINESIN-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15605">
        <property type="entry name" value="KINESIN-ASSOCIATED PROTEINS"/>
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        <property type="entry name" value="KAP"/>
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    </dbReference>
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        <property type="entry name" value="ARM"/>
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    </dbReference>
    <dbReference type="SMART" id="SM01297">
        <property type="entry name" value="KAP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM repeat"/>
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    </dbReference>
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        <property type="entry name" value="ARM_REPEAT"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ARM" evidence="1">
        <location>
            <begin position="592"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="241"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053543194.1"/>
        </source>
    </evidence>
    <sequence length="769" mass="88468" checksum="DF4E82BB5F8B94DC" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>E3TF85</accession>
    <name>E3TF85_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="17 19">Coagulation factor VII</fullName>
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    </protein>
    <gene>
        <name evidence="17" type="primary">FA7</name>
        <name evidence="19" type="synonym">f7i</name>
        <name evidence="19" type="synonym">fa7</name>
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    <organism evidence="17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="17 19" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.21" evidence="19"/>
    <dbReference type="EMBL" id="GU589019">
        <property type="protein sequence ID" value="ADO28971.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187676.1">
        <property type="nucleotide sequence ID" value="NM_001200747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TF85"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000016294"/>
    <dbReference type="GeneID" id="100528477"/>
    <dbReference type="KEGG" id="ipu:100528477"/>
    <dbReference type="CTD" id="282671"/>
    <dbReference type="OrthoDB" id="10004439at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000013">
        <property type="entry name" value="Coagulation factor X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000255">
        <property type="entry name" value="Sushi, nidogen and EGF-like domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.740.10:FF:000001">
        <property type="entry name" value="vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.740.10">
        <property type="entry name" value="Coagulation Factor IX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017857">
        <property type="entry name" value="Coagulation_fac-like_Gla_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035972">
        <property type="entry name" value="GLA-like_dom_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000294">
        <property type="entry name" value="GLA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012224">
        <property type="entry name" value="Pept_S1A_FX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050442">
        <property type="entry name" value="Peptidase_S1_coag_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278">
        <property type="entry name" value="COAGULATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278:SF26">
        <property type="entry name" value="COAGULATION FACTOR VII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00594">
        <property type="entry name" value="Gla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001143">
        <property type="entry name" value="Factor_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00001">
        <property type="entry name" value="GLABLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00069">
        <property type="entry name" value="GLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57630">
        <property type="entry name" value="GLA-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00011">
        <property type="entry name" value="GLA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50998">
        <property type="entry name" value="GLA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 12" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 13 19" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5011323948" evidence="13 19">
        <location>
            <begin position="19"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Gla" evidence="16">
        <location>
            <begin position="37"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="83"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="15">
        <location>
            <begin position="186"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ADO28971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="NP_001187676.1"/>
        </source>
    </evidence>
    <sequence length="456" mass="50475" checksum="089832B621EB8B40" modified="2011-01-11" version="1">MKSETFLVLCLFCHAVSGAVFLRRNDANSVLRRFRRANSGLLEELKKGNLERECVEEICDYEEAREVFEDEAITKQFWISYHAREPCLTNPCKNNGTCIYLGHSYICQCSEGFEGKYCQEAFEDTLKCLYLNGGCEQFCDGSAWKRKCACAPGYALGEDKKSCIAQVQYPCGKAPLEITQTVLPRAVGGNQCPKGHYPWQVLLKYDGQSLCGGVLLDTNWILTAAHCVAKKDIKKLKVIAGDHNIDQVEGTEQTFSVSHVIIHESYDPASADSDLALLRLSEPATLSNHTIPICLPTSEFARMELEAVRFHTVSGWGQHTVGGNVRTSQSVKSIAPVLRTLAVPFLPKPECSIKSGVNITDNMLCTGYFEGSQESCRGDDGSPLTTQYKDTHFLTGIVSWGKGCAHPGFYAIYTKVANFLDWLQRGMATPIAHPDAFNTSRASVMTTPPAEQFTWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5U7P6</accession>
    <name>W5U7P6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nucleophosmin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">npm1</name>
        <name evidence="16" type="synonym">npm1a</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the nucleoplasmin family.</text>
    </comment>
    <dbReference type="EMBL" id="JT407544">
        <property type="protein sequence ID" value="AHH38021.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017345472.1">
        <property type="nucleotide sequence ID" value="XM_017489983.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U7P6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000028757"/>
    <dbReference type="GeneID" id="108277343"/>
    <dbReference type="KEGG" id="ipu:108277343"/>
    <dbReference type="CTD" id="266985"/>
    <dbReference type="GeneTree" id="ENSGT00940000166176"/>
    <dbReference type="OrthoDB" id="6075101at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030097">
        <property type="term" value="P:hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010824">
        <property type="term" value="P:regulation of centrosome duplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2100:FF:000001">
        <property type="entry name" value="Nucleophosmin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.340:FF:000007">
        <property type="entry name" value="Nucleophosmin 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.340">
        <property type="entry name" value="Nucleoplasmin core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032569">
        <property type="entry name" value="NPM1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004301">
        <property type="entry name" value="Nucleoplasmin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024057">
        <property type="entry name" value="Nucleoplasmin_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036824">
        <property type="entry name" value="Nucleoplasmin_core_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22747:SF42">
        <property type="entry name" value="NUCLEOPHOSMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22747">
        <property type="entry name" value="NUCLEOPLASMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16276">
        <property type="entry name" value="NPM1-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03066">
        <property type="entry name" value="Nucleoplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69203">
        <property type="entry name" value="Nucleoplasmin-like core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Nucleoplasmin core" evidence="12">
        <location>
            <begin position="12"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleophosmin C-terminal" evidence="13">
        <location>
            <begin position="241"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="117"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="118"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="151"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="198"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="224"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AHH38021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017345472.1"/>
        </source>
    </evidence>
    <sequence length="292" mass="32516" checksum="4D7262AC398ED7A8" modified="2014-04-16" version="1">MDAEQMGPQTFLYGCELKQGKEVTFNPEDDDFEHQLSVRMACVDPATKDELNVVEVEGQDSEGQKVKAVLATLKPSTMPSVCLGGFEVSPPVVFRLRTGSGPVHISGQHLIMMSGDQSFDEDDEEEEEDMRTAVKRGAPAAAKPSKKMKMEEEEEDDEEDDDDEDDEDDEEEEEEESEEETPVKAKKPAAKPQTPAQNGKSNTPAPKQNKTPNTGKKNEKKPQTPKSPKTPKSPQTPLSVPEIKAKMMASIEKGVMLPKIQPKFENYVKHGFKVTDVKVIGELWQWRQTVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5U953</accession>
    <name>W5U953_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytochrome c oxidase assembly protein COX20, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cox20</name>
        <name evidence="12" type="synonym">LOC108263832</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COX20 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT409048">
        <property type="protein sequence ID" value="AHH38541.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017320489.1">
        <property type="nucleotide sequence ID" value="XM_017465000.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U953"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000023024"/>
    <dbReference type="GeneID" id="108263832"/>
    <dbReference type="KEGG" id="ipu:108263832"/>
    <dbReference type="OrthoDB" id="14603at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022533">
        <property type="entry name" value="Cox20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31586:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX20, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31586">
        <property type="entry name" value="CYTOCHROME C OXIDASE PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12597">
        <property type="entry name" value="Cox20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02049">
        <property type="entry name" value="PROTEINF36A"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AHH38541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017320489.1"/>
        </source>
    </evidence>
    <sequence length="112" mass="12345" checksum="AB16C2FE6E3D4962" modified="2014-04-16" version="1">MAEQEPDSKNQGVKLLGILDVGKTPCAREAILHGAGGSAVAGLVHFLATSRVKRSFHVGMAGFMLTTLASWFYCRYNNARLRVQQKTIQEGIKNKVIYEGTSHDPTRKARDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5U965</accession>
    <name>W5U965_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">KIF18A</name>
        <name evidence="17" type="synonym">kif18a</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="JT408478">
        <property type="protein sequence ID" value="AHH38350.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017314695.1">
        <property type="nucleotide sequence ID" value="XM_017459206.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U965"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026795"/>
    <dbReference type="GeneID" id="108259595"/>
    <dbReference type="KEGG" id="ipu:108259595"/>
    <dbReference type="CTD" id="81930"/>
    <dbReference type="OMA" id="QPMMSAV"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01370">
        <property type="entry name" value="KISc_KIP3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000027">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF65">
        <property type="entry name" value="KINESIN MOTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 11 12" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="14">
        <location>
            <begin position="9"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="644"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="812"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="669"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="111"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AHH38350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017314695.1"/>
        </source>
    </evidence>
    <sequence length="905" mass="101735" checksum="BBEF9EA7BA259B16" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5UD67</accession>
    <name>W5UD67_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">Mapkapk2</name>
        <name evidence="16" type="synonym">mapkapk2a</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JT412334">
        <property type="protein sequence ID" value="AHH39805.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017306290.1">
        <property type="nucleotide sequence ID" value="XM_017450801.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UD67"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035884"/>
    <dbReference type="GeneID" id="108255116"/>
    <dbReference type="KEGG" id="ipu:108255116"/>
    <dbReference type="CTD" id="327321"/>
    <dbReference type="OMA" id="FPQFHIK"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14170">
        <property type="entry name" value="STKc_MAPKAPK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000094">
        <property type="entry name" value="MAP kinase-activated protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000156">
        <property type="entry name" value="MAP kinase-activated protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1170.10:FF:000001">
        <property type="entry name" value="MAP kinase-activated protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1170.10">
        <property type="entry name" value="MAP kinase activated protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027442">
        <property type="entry name" value="MAPKAPK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 14 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="42"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AHH39805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017306290.1"/>
        </source>
    </evidence>
    <sequence length="379" mass="43642" checksum="9B4843918A4468AA" modified="2014-04-16" version="1">MLSNSQNQLVFPNQPQQQQQHLQFHAKPALQIKKNAITDDYKVTNQVLGLGINGKVLEIVQKKNGEKYALKMLQDCPKARREVELHWRASPCTHIVRIIDVYENLYQGRKCLLIVMECMDGGELFSRIQDRGDQAFTEREASDIMKSIGEAILFLHSINIAHRDVKPENLLYTSKRPNAVLKLTDFGFAKETTTLNSLTTPCYTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGYPPFYSNHGLAISPGMKKRIRMGQYEFPNPEWSEVSEEAKHLIKRLLKTEPSMRITISEFMNHPWINQSMEVPQTPLHTSRVLKEEKDVWEEVKEEMTSALATMRVDYEQIKIKTIEDSSNPLLMKRRKKANMAAENQTSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5UDN8</accession>
    <name>W5UDN8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Dymeclin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7 8" type="primary">dym</name>
    </gene>
    <organism evidence="5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
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                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
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                <person name="Sonstegard T."/>
                <person name="Feng J."/>
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                <person name="Rajendran K.V."/>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
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                <person name="Liu S."/>
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                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
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                <person name="Rajendran K.V."/>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="JT415071">
        <property type="protein sequence ID" value="AHH40909.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017336677.1">
        <property type="nucleotide sequence ID" value="XM_017481188.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000010787"/>
    <dbReference type="GeneID" id="108272646"/>
    <dbReference type="KEGG" id="ipu:108272646"/>
    <dbReference type="CTD" id="100006039"/>
    <dbReference type="GeneTree" id="ENSGT00940000156524"/>
    <dbReference type="OMA" id="PKVPSCQ"/>
    <dbReference type="OrthoDB" id="7312725at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045914">
        <property type="entry name" value="Zn532-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041697">
        <property type="entry name" value="Znf-C2H2_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057356">
        <property type="entry name" value="Znf-C2H2_ZNF592"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47222">
        <property type="entry name" value="ZINC FINGER PROTEIN 532-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47222:SF2">
        <property type="entry name" value="ZINC FINGER PROTEIN 687"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16622">
        <property type="entry name" value="zf-C2H2_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25412">
        <property type="entry name" value="zf-C2H2_ZNF592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="678"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="986"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="1017"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="1143"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="1307"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="1362"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="23"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="89"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="176"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="506"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="758"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1041"/>
            <end position="1112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1201"/>
            <end position="1272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1331"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="113"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="220"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="251"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="341"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="362"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="399"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="764"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="795"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1049"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1073"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1332"/>
            <end position="1351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AHH40909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017336677.1"/>
        </source>
    </evidence>
    <sequence length="1398" mass="150177" checksum="BC608777D0A8C772" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5UL39</accession>
    <name>W5UL39_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8 10">CKLF-like MARVEL transmembrane domain-containing protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CMTM3</name>
        <name evidence="10" type="synonym">cmtm3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT418381">
        <property type="protein sequence ID" value="AHH42716.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017313983.1">
        <property type="nucleotide sequence ID" value="XM_017458494.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UL39"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020873"/>
    <dbReference type="GeneID" id="108259226"/>
    <dbReference type="KEGG" id="ipu:108259226"/>
    <dbReference type="CTD" id="123920"/>
    <dbReference type="OMA" id="CFPLMDF"/>
    <dbReference type="OrthoDB" id="9943862at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050578">
        <property type="entry name" value="MARVEL-CKLF_proteins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776:SF3">
        <property type="entry name" value="CKLF-LIKE MARVEL TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776">
        <property type="entry name" value="MARVEL-CONTAINING POTENTIAL LIPID RAFT-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="7">
        <location>
            <begin position="26"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AHH42716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017313983.1"/>
        </source>
    </evidence>
    <sequence length="170" mass="18635" checksum="370F889D79705A34" modified="2014-04-16" version="1">MGDIESPDRSESRAAQVFSLLMSKELIGSRKGQLLLAEVISSFISFVCFCASTAAAFITAPLLAFISALLILFAHAIKFNQRFKGFHWPLMDFLRCITASIVFFIISIMAVSRYPDGSSKAAGVFGFVATIVFALDFYFIFNELADFLKGGGSNEEPQNKQDADSDLDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5UMG2</accession>
    <name>W5UMG2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6 8">U11/U12 small nuclear ribonucleoprotein 48 kDa protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">Snrnp48</name>
        <name evidence="8" type="synonym">snrnp48</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT418921">
        <property type="protein sequence ID" value="AHH42895.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017333351.1">
        <property type="nucleotide sequence ID" value="XM_017477862.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UMG2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000031179"/>
    <dbReference type="GeneID" id="108270881"/>
    <dbReference type="CTD" id="154007"/>
    <dbReference type="OMA" id="DPNHQMP"/>
    <dbReference type="OrthoDB" id="69229at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005689">
        <property type="term" value="C:U12-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022776">
        <property type="entry name" value="TRM13/UPF0224_CHHC_Znf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051591">
        <property type="entry name" value="UPF0224_FAM112_RNA_Proc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21402">
        <property type="entry name" value="GAMETOCYTE SPECIFIC FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21402:SF10">
        <property type="entry name" value="U11_U12 SMALL NUCLEAR RIBONUCLEOPROTEIN 48 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51800">
        <property type="entry name" value="ZF_CHHC_U11_48K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHHC U11-48K-type" evidence="5">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="253"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="268"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="301"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="324"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AHH42895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017333351.1"/>
        </source>
    </evidence>
    <sequence length="335" mass="39095" checksum="89066DF040E8D5EE" modified="2014-04-16" version="1">MSDFDSSEALQDRLQALHQLTEFTQNCRTQLEELLEVLGWSWENYSPQDKMEVCPHDPNHIVPCRSMENHKATCLLGQLGYSKEEQAEMYDTLFCYERANIPSIKLDKLAQHQVILQARASAPPVQSTGNYCQKDYSTEPADVPQNHKRTICDLTAADRLALYDHVIQEVIQQSAKASSNEDLYVDLVSRLNKDEEKSGPKSHLEVLAEMRDYRRRRQSYRAKNVHITKKSYTEIIREVIDVHSGELAKLWQEEREEETKTSQHSHRRLPDERRSASVESRESHVSSREENSSRHKHHQKHSCEHSQERGSKSRRRDSHSPEPRHHKKKKKKNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5URS2</accession>
    <accession>A0A2D0T2S5</accession>
    <name>W5URS2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Integrin beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="31" type="primary">Itgb3</name>
        <name evidence="33" type="synonym">itgb3a</name>
    </gene>
    <organism evidence="31">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="31">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="32" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="32">SDA103</strain>
        </source>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2023-07" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="33">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="25">Cell membrane</location>
            <topology evidence="25">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Lamellipodium membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="23">Postsynaptic cell membrane</location>
            <topology evidence="23">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 25">Belongs to the integrin beta chain family.</text>
    </comment>
    <dbReference type="EMBL" id="JT417271">
        <property type="protein sequence ID" value="AHH42323.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017349218.2">
        <property type="nucleotide sequence ID" value="XM_017493729.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5URS2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000036089"/>
    <dbReference type="GeneID" id="108279461"/>
    <dbReference type="KEGG" id="ipu:108279461"/>
    <dbReference type="CTD" id="564266"/>
    <dbReference type="OrthoDB" id="410592at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031258">
        <property type="term" value="C:lamellipodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070051">
        <property type="term" value="F:fibrinogen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001968">
        <property type="term" value="F:fibronectin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070527">
        <property type="term" value="P:platelet aggregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045124">
        <property type="term" value="P:regulation of bone resorption"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.100:FF:000002">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000036">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000075">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000002">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000002">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.30:FF:000001">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013111">
        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040622">
        <property type="entry name" value="EGF_integrin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057073">
        <property type="entry name" value="EGF_integrin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033760">
        <property type="entry name" value="Integrin_beta_N"/>
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    <dbReference type="InterPro" id="IPR015812">
        <property type="entry name" value="Integrin_bsu"/>
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    <dbReference type="InterPro" id="IPR014836">
        <property type="entry name" value="Integrin_bsu_cyt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012896">
        <property type="entry name" value="Integrin_bsu_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036349">
        <property type="entry name" value="Integrin_bsu_tail_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002369">
        <property type="entry name" value="Integrin_bsu_VWA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057243">
        <property type="entry name" value="Integrin_I-EGF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082">
        <property type="entry name" value="INTEGRIN BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082:SF25">
        <property type="entry name" value="INTEGRIN BETA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07974">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23105">
        <property type="entry name" value="EGF_integrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18372">
        <property type="entry name" value="I-EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08725">
        <property type="entry name" value="Integrin_b_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07965">
        <property type="entry name" value="Integrin_B_tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00362">
        <property type="entry name" value="Integrin_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17205">
        <property type="entry name" value="PSI_integrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002512">
        <property type="entry name" value="Integrin_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01186">
        <property type="entry name" value="INTEGRINB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00187">
        <property type="entry name" value="INB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01241">
        <property type="entry name" value="Integrin_b_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01242">
        <property type="entry name" value="Integrin_B_tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69687">
        <property type="entry name" value="Integrin beta tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00243">
        <property type="entry name" value="I_EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52047">
        <property type="entry name" value="I_EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="13 25" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="14" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="22" id="KW-0966">Cell projection</keyword>
    <keyword evidence="19 24" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="20" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17 25 31" id="KW-0401">Integrin</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 27" id="KW-0732">Signal</keyword>
    <keyword evidence="16" id="KW-0770">Synapse</keyword>
    <keyword evidence="7 25 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 26" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="27">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044739794" description="Integrin beta" evidence="27">
        <location>
            <begin position="24"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="717"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin beta subunit VWA" evidence="28">
        <location>
            <begin position="34"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin beta subunit tail" evidence="30">
        <location>
            <begin position="631"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin beta subunit cytoplasmic" evidence="29">
        <location>
            <begin position="740"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="27"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="38"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="48"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="202"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="257"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="399"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="431"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="458"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="473"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="480"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="485"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="524"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="526"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="531"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="546"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="565"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="567"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="572"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="583"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="604"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="606"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="611"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="624"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="631"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="637"/>
            <end position="711"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007449"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00035006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR002512-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU000633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="SMART" id="SM00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="SMART" id="SM01241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="SMART" id="SM01242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="EMBL" id="AHH42323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="RefSeq" id="XP_017349218.2"/>
        </source>
    </evidence>
    <sequence length="787" mass="85813" checksum="D1580B506692591F" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5UT39</accession>
    <name>W5UT39_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome c oxidase subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">COX42</name>
        <name evidence="13 14 15 16" type="synonym">LOC108275904</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="11">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13 14 15" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13 14 15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of 14 subunits. The complex is composed of a catalytic core of 3 subunits MT-CO1, MT-CO2 and MT-CO3, encoded in the mitochondrial DNA, and 11 supernumerary subunits COX4I, COX5A, COX5B, COX6A, COX6B, COX6C, COX7A, COX7B, COX7C, COX8 and NDUFA4, which are encoded in the nuclear genome. The complex exists as a monomer or a dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), resulting in different assemblies (supercomplex SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion inner membrane</location>
            <topology evidence="1 10">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the cytochrome c oxidase IV family.</text>
    </comment>
    <dbReference type="EMBL" id="JT418672">
        <property type="protein sequence ID" value="AHH42808.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017342491.1">
        <property type="nucleotide sequence ID" value="XM_017487002.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017342492.1">
        <property type="nucleotide sequence ID" value="XM_017487003.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017342493.1">
        <property type="nucleotide sequence ID" value="XM_017487004.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017342494.1">
        <property type="nucleotide sequence ID" value="XM_017487005.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UT39"/>
    <dbReference type="GeneID" id="108275904"/>
    <dbReference type="OMA" id="IDCADFP"/>
    <dbReference type="OrthoDB" id="186013at2759"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00922">
        <property type="entry name" value="Cyt_c_Oxidase_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.442.10:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 4 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.442.10">
        <property type="entry name" value="Cytochrome c oxidase subunit IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013288">
        <property type="entry name" value="Cyt_c_oxidase_su4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004203">
        <property type="entry name" value="Cyt_c_oxidase_su4_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036639">
        <property type="entry name" value="Cyt_c_oxidase_su4_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10707:SF15">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10707">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02936">
        <property type="entry name" value="COX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01873">
        <property type="entry name" value="CYTCOXIDASE4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81406">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AHH42808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017342491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017342492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017342493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017342494.1"/>
        </source>
    </evidence>
    <sequence length="173" mass="20135" checksum="090C0FB57A5E9591" modified="2014-04-16" version="1">MLCLTAGRLGTLLSRRAVAALGTTTARMCSHVSQQADMSLPMYWDRRDIPLPDRPYNDSLTATEKTLKQKEKGPWNILSNEEKLALYRIMFKDTYAEMKKPSSEWKTVLGGLFFFIGLTGLVVLWQRIYVYPHPPHTFDEEWEAKQVKRMLDMRVNPVQGFSAKWDYEKGQWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PKY4</accession>
    <name>A0A2D0PKY4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 14">Uracil-DNA glycosylase</fullName>
            <shortName evidence="12">UDG</shortName>
            <ecNumber evidence="12 14">3.2.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">unga</name>
        <name evidence="12" type="synonym">UNG</name>
        <name evidence="12" type="synonym">UNG1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 14">Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.</text>
            <dbReference type="EC" id="3.2.2.27"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 2'-deoxyuridine in double-stranded DNA + H2O = a 2'-deoxyribose 5'-monophosphate in double-stranded DNA + uracil</text>
            <dbReference type="Rhea" id="RHEA:81455"/>
            <dbReference type="Rhea" id="RHEA-COMP:14231"/>
            <dbReference type="Rhea" id="RHEA-COMP:17071"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:133902"/>
            <dbReference type="ChEBI" id="CHEBI:139095"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:81456"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 2'-deoxyuridine in single-stranded DNA + H2O = a 2'-deoxyribose 5'-monophosphate in single-stranded DNA + uracil</text>
            <dbReference type="Rhea" id="RHEA:81459"/>
            <dbReference type="Rhea" id="RHEA-COMP:12847"/>
            <dbReference type="Rhea" id="RHEA-COMP:19684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:133902"/>
            <dbReference type="ChEBI" id="CHEBI:139095"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:81460"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with RPA2 subunit of the RPA trimer; this interaction mediates UNG2 recruitment to RPA-coated single-stranded DNA at stalled replication forks. Interacts with PCNA; this interaction mediates UNG2 recruitment to S-phase replication foci. Interacts (via N-terminus) with FAM72A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 12 14">Belongs to the uracil-DNA glycosylase (UDG) superfamily. UNG family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.27" evidence="12 14"/>
    <dbReference type="RefSeq" id="XP_017306682.1">
        <property type="nucleotide sequence ID" value="XM_017451193.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PKY4"/>
    <dbReference type="GeneID" id="108255309"/>
    <dbReference type="KEGG" id="ipu:108255309"/>
    <dbReference type="CTD" id="393949"/>
    <dbReference type="OrthoDB" id="10031947at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097510">
        <property type="term" value="P:base-excision repair, AP site formation via deaminated base removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10027">
        <property type="entry name" value="UDG-F1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.470.10:FF:000004">
        <property type="entry name" value="Uracil-DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00148">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002043">
        <property type="entry name" value="UDG_fam1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018085">
        <property type="entry name" value="Ura-DNA_Glyclase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003588">
        <property type="entry name" value="PRK05254.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003589">
        <property type="entry name" value="PRK05254.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003591">
        <property type="entry name" value="PRK05254.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003592">
        <property type="entry name" value="PRK05254.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00628">
        <property type="entry name" value="ung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11264">
        <property type="entry name" value="URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11264:SF0">
        <property type="entry name" value="URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00987">
        <property type="entry name" value="UreE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00130">
        <property type="entry name" value="U_DNA_GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3 12 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 12 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 12 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="15">
        <location>
            <begin position="122"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12 13">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017306682.1"/>
        </source>
    </evidence>
    <sequence length="296" mass="33721" checksum="F3009AFAD5DFCD24" modified="2017-12-20" version="1">MAVLFINSQLRFLHTPCPRVSSKRWWNCSKYTRISSQVKKRKAGDDVLLSPEQLERIARNKAAALERLSSRRGVVGVGESWRRALGAEFNKHYFTSLMDFVDNERQKHTVYPPLEQVFTWTQMCQIEDVKVVVLGQDPYHGPNQAHGLCFSVQRPERPPPSLVNMYKELESDIEGFHHPGHGDLTGWAKQGVLLLNAVLTVRAHQANSHKDKGWETFTDAVVQWLSTNLEGLVFMLWGSYAQKKGAAIDRKRHHVLQTVHPSPLSAHRGFLGCKHFSKTNELLLKSGKKPIDWKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PLJ6</accession>
    <name>A0A2D0PLJ6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">mapkapk2a</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017306291.1">
        <property type="nucleotide sequence ID" value="XM_017450802.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PLJ6"/>
    <dbReference type="GeneID" id="108255116"/>
    <dbReference type="CTD" id="327321"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14170">
        <property type="entry name" value="STKc_MAPKAPK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000094">
        <property type="entry name" value="MAP kinase-activated protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000156">
        <property type="entry name" value="MAP kinase-activated protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1170.10:FF:000001">
        <property type="entry name" value="MAP kinase-activated protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1170.10">
        <property type="entry name" value="MAP kinase activated protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027442">
        <property type="entry name" value="MAPKAPK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="42"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017306291.1"/>
        </source>
    </evidence>
    <sequence length="378" mass="43514" checksum="B955CAE5DE0BF117" modified="2017-12-20" version="1">MLSNSQNQLVFPNQPQQQQQHLQFHAKPALQIKKNAITDDYKVTNQVLGLGINGKVLEIVQKKNGEKYALKMLQDCPKARREVELHWRASPCTHIVRIIDVYENLYQGRKCLLIVMECMDGGELFSRIQDRGDQAFTEREASDIMKSIGEAILFLHSINIAHRDVKPENLLYTSKRPNAVLKLTDFGFAKETTTLNSLTTPCYTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGYPPFYSNHGLAISPGMKKRIRMGQYEFPNPEWSEVSEEAKHLIKRLLKTEPSMRITISEFMNHPWINSMEVPQTPLHTSRVLKEEKDVWEEVKEEMTSALATMRVDYEQIKIKTIEDSSNPLLMKRRKKANMAAENQTSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PM55</accession>
    <name>A0A2D0PM55_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">slc6a4b</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017307084.1">
        <property type="nucleotide sequence ID" value="XM_017451595.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PM55"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034433"/>
    <dbReference type="GeneID" id="108255560"/>
    <dbReference type="KEGG" id="ipu:108255560"/>
    <dbReference type="CTD" id="664770"/>
    <dbReference type="OMA" id="YMAEQRH"/>
    <dbReference type="OrthoDB" id="6581954at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051378">
        <property type="term" value="F:serotonin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005335">
        <property type="term" value="F:serotonin:sodium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF129">
        <property type="entry name" value="TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00754">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="107"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="227"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="273"/>
            <end position="297"/>
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                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017309029.1">
        <property type="nucleotide sequence ID" value="XM_017453540.3"/>
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    <dbReference type="KEGG" id="ipu:108256552"/>
    <dbReference type="CTD" id="25914"/>
    <dbReference type="OrthoDB" id="428850at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
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    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007099">
        <property type="term" value="P:centriole replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:centriole-centriole cohesion"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:ciliary basal body organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
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    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030791">
        <property type="entry name" value="Rotatin"/>
    </dbReference>
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        <property type="entry name" value="Rotatin_N"/>
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        <property type="entry name" value="ROTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31691:SF1">
        <property type="entry name" value="ROTATIN"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <begin position="16"/>
            <end position="112"/>
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    </feature>
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        <location>
            <begin position="280"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1554"/>
            <end position="1575"/>
        </location>
    </feature>
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        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="333"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017309029.1"/>
        </source>
    </evidence>
    <sequence length="2191" mass="242407" checksum="BBDF1D2D0367541C" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PT56</accession>
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        <submittedName>
            <fullName evidence="4">Protein CDV3 homolog isoform X1</fullName>
        </submittedName>
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    <gene>
        <name evidence="4" type="primary">cdv3</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
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            <taxon>Neopterygii</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDV3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017308611.1">
        <property type="nucleotide sequence ID" value="XM_017453122.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PT56"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006576"/>
    <dbReference type="GeneID" id="108256354"/>
    <dbReference type="KEGG" id="ipu:108256354"/>
    <dbReference type="CTD" id="55573"/>
    <dbReference type="OMA" id="TSGPWNK"/>
    <dbReference type="OrthoDB" id="6288097at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026806">
        <property type="entry name" value="CDV3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16284">
        <property type="entry name" value="PROTEIN CDV3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16284:SF13">
        <property type="entry name" value="PROTEIN CDV3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15359">
        <property type="entry name" value="CDV3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="88"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="93"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017308611.1"/>
        </source>
    </evidence>
    <sequence length="231" mass="25716" checksum="35AB7D72CD8861F1" modified="2017-12-20" version="1">MAEVATPGAEKSLDDFFAKRDKKKKKEKGKGKEVGPVIAPASLKKTKKEKEKSMKNENQDPTIEKEDEEWKEFEQKEVDYTGLRLQALMMSDEKEEEEYEKEEVGEDGEIILVSGDKVSGPWNKSGPPPPAAPVAEDKEVSESKPAGVYRPPGARLGSSKKGPSQGPPEIFNDAQFPSLLATAKHVETRKDREIEKTFEVVKHKSRAREEGGQGAVQHLQLDNQFAVLEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PWU2</accession>
    <name>A0A2D0PWU2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Angiopoietin-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">angpt1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="11">Homooligomer. Interacts with TEK/TIE2. Interacts with SVEP1/polydom. Interacts with THBD; this interaction significantly inhibits the generation of activated PC and TAFIa/CPB2 by the thrombin/thrombomodulin complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017310492.1">
        <property type="nucleotide sequence ID" value="XM_017455003.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PWU2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022359"/>
    <dbReference type="GeneID" id="108257334"/>
    <dbReference type="KEGG" id="ipu:108257334"/>
    <dbReference type="CTD" id="284"/>
    <dbReference type="OMA" id="QYNANAL"/>
    <dbReference type="OrthoDB" id="7735366at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.215.10:FF:000005">
        <property type="entry name" value="angiopoietin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.530.10:FF:000001">
        <property type="entry name" value="angiopoietin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.530.10">
        <property type="entry name" value="Gamma-fibrinogen Carboxyl Terminal Fragment, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057439">
        <property type="entry name" value="ANG-1/2/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037579">
        <property type="entry name" value="FIB_ANG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020837">
        <property type="entry name" value="Fibrinogen_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221:SF6">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25443">
        <property type="entry name" value="ANG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00514">
        <property type="entry name" value="FIBRINOGEN_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013333902" description="Angiopoietin-1" evidence="14">
        <location>
            <begin position="23"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="15">
        <location>
            <begin position="307"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="28"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="213"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017310492.1"/>
        </source>
    </evidence>
    <sequence length="528" mass="59653" checksum="B3963CB2BEEDD426" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PYC1</accession>
    <name>A0A2D0PYC1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Cell death-inducing p53-target protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC108257145</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Late endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lysosome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017310115.1">
        <property type="nucleotide sequence ID" value="XM_017454626.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PYC1"/>
    <dbReference type="GeneID" id="108257145"/>
    <dbReference type="KEGG" id="ipu:108257145"/>
    <dbReference type="OrthoDB" id="4713066at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098560">
        <property type="term" value="C:cytoplasmic side of late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098574">
        <property type="term" value="C:cytoplasmic side of lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF35">
        <property type="entry name" value="LITAF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="156"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="LITAF" evidence="10">
        <location>
            <begin position="117"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="10"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017310115.1"/>
        </source>
    </evidence>
    <sequence length="202" mass="22888" checksum="319EE77BAFC311E8" modified="2017-12-20" version="1">MAATSKQQKLQRINEELHKLTLHRDQLNSRLRLLALLKEFRQNAGYHAEAVGEPTENDEYERIQEELKALSERKLALQALRETLEGTSKSCDTLSTPQKYDPSGNEIFIVERPPSYPAPQVISDIQKLPRHPSQIQCPYCEQYITTEVSTVIGNTTWLVCLVSTFIGCVAGCCLIPFCISAFKDVVHKCPKCRSHIHTCTKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q9R7</accession>
    <name>A0A2D0Q9R7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Probable ribonuclease ZC3H12B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">zc3h12b</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZC3H12 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017315042.1">
        <property type="nucleotide sequence ID" value="XM_017459553.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q9R7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000024765"/>
    <dbReference type="GeneID" id="108259837"/>
    <dbReference type="KEGG" id="ipu:108259837"/>
    <dbReference type="CTD" id="340554"/>
    <dbReference type="OMA" id="WDPLPCT"/>
    <dbReference type="OrthoDB" id="392925at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036464">
        <property type="term" value="C:cytoplasmic ribonucleoprotein granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18729">
        <property type="entry name" value="PIN_Zc3h12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11980:FF:000001">
        <property type="entry name" value="ZC3H12A isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056629">
        <property type="entry name" value="KH_N4BP1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040546">
        <property type="entry name" value="Rege-1_UBA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040757">
        <property type="entry name" value="Regnase_1/ZC3H12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021869">
        <property type="entry name" value="RNase_Zc3h12_NYN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051101">
        <property type="entry name" value="ZC3H12/N4BP1_RNase_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12876">
        <property type="entry name" value="N4BP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12876:SF27">
        <property type="entry name" value="RIBONUCLEASE ZC3H12B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23050">
        <property type="entry name" value="KH_N4BP1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18561">
        <property type="entry name" value="Regnase_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11977">
        <property type="entry name" value="RNase_Zc3h12a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18039">
        <property type="entry name" value="UBA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="485"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="485"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="39"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="116"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="275"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="599"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="849"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="116"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="129"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="202"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017315042.1"/>
        </source>
    </evidence>
    <sequence length="982" mass="107914" checksum="563144FFCEFAA4F1" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QBW6</accession>
    <name>A0A2D0QBW6_ICTPU</name>
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        <submittedName>
            <fullName evidence="6">Tubulin polyglutamylase TTLL11 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ttll11</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017314883.1">
        <property type="nucleotide sequence ID" value="XM_017459394.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QBW6"/>
    <dbReference type="GeneID" id="108259700"/>
    <dbReference type="KEGG" id="ipu:108259700"/>
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    <dbReference type="OrthoDB" id="202825at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070740">
        <property type="term" value="F:tubulin-glutamic acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241:SF154">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE TTLL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="625"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017314883.1"/>
        </source>
    </evidence>
    <sequence length="681" mass="76047" checksum="8846B8CF33A3A658" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QC79</accession>
    <name>A0A2D0QC79_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mannosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">alg9</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycosyltransferase 22 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="RefSeq" id="XP_017315316.1">
        <property type="nucleotide sequence ID" value="XM_017459827.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QC79"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033596"/>
    <dbReference type="GeneID" id="108259968"/>
    <dbReference type="KEGG" id="ipu:108259968"/>
    <dbReference type="CTD" id="79796"/>
    <dbReference type="OMA" id="PRDMHAK"/>
    <dbReference type="OrthoDB" id="497541at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF2">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE ALG9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="173"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="219"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="255"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="315"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="351"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="375"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="415"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017315316.1"/>
        </source>
    </evidence>
    <sequence length="615" mass="70506" checksum="E0850F26F32A472F" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QD93</accession>
    <name>A0A2D0QD93_ICTPU</name>
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        <submittedName>
            <fullName evidence="4">BMERB domain-containing protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">zgc:171844</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017315686.1">
        <property type="nucleotide sequence ID" value="XM_017460197.3"/>
    </dbReference>
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    <dbReference type="GeneID" id="108260146"/>
    <dbReference type="KEGG" id="ipu:108260146"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="entry name" value="BMERB"/>
    </dbReference>
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        <property type="entry name" value="bMERB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22704:SF2">
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017315686.1"/>
        </source>
    </evidence>
    <sequence length="196" mass="22776" checksum="F5C1A3F2217CB01A" modified="2017-12-20" version="1">MQKERRCGKLYGSVSRVDPGEDAHRTVPPSDEDDVVSMADSTITVDDIEGELFKIERIRDVLVRRESELRYMMDDIQLCKEITRLKKELQKLVSIPDKDKSSEDRQREEELLQQIHKLVETRDFLVDDVEFERLREQEEDKEMAEFLRAKFPASTTKKISTQKRRMTSRAQVSSPKVTKAGLTLLKECCGFTCSVM</sequence>
</entry>
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    <accession>A0A2D0QDL9</accession>
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        <name evidence="12 13" type="primary">ftr82</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
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                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
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                <person name="Sonstegard T."/>
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                <person name="Danzmann R.G."/>
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                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
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                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12 13" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12 13">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017315811.1">
        <property type="nucleotide sequence ID" value="XM_017460322.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047007710.1">
        <property type="nucleotide sequence ID" value="XM_047151754.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QDL9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012584"/>
    <dbReference type="GeneID" id="108260233"/>
    <dbReference type="KEGG" id="ipu:108260233"/>
    <dbReference type="CTD" id="338307"/>
    <dbReference type="GeneTree" id="ENSGT00940000166102"/>
    <dbReference type="OMA" id="GFIPKVT"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19802">
        <property type="entry name" value="Bbox1_TRIM8-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16040">
        <property type="entry name" value="SPRY_PRY_SNTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001655">
        <property type="entry name" value="FinTRIM family, member 82"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="B30.2/SPRY"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
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    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF30">
        <property type="entry name" value="FINTRIM FAMILY, MEMBER 82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
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    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="12"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="9">
        <location>
            <begin position="149"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="10">
        <location>
            <begin position="388"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="211"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="269"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017315811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_047007710.1"/>
        </source>
    </evidence>
    <sequence length="576" mass="65098" checksum="41A3708C79B9B7E9" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QE92</accession>
    <name>A0A2D0QE92_ICTPU</name>
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        <submittedName>
            <fullName evidence="5">CAP-Gly domain-containing linker protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">clip2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017316046.1">
        <property type="nucleotide sequence ID" value="XM_017460557.3"/>
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    <dbReference type="GeneID" id="108260333"/>
    <dbReference type="KEGG" id="ipu:108260333"/>
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    <dbReference type="OrthoDB" id="5412539at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
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    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Putative CAP-Gly domain-containing linker protein 1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
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    </dbReference>
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        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916:SF10">
        <property type="entry name" value="CAP-GLY DOMAIN-CONTAINING LINKER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916">
        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
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    </dbReference>
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        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="92"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="CAP-Gly" evidence="3">
        <location>
            <begin position="223"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="137"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="177"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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    </feature>
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        <location>
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    </feature>
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        <location>
            <begin position="50"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="177"/>
            <end position="193"/>
        </location>
    </feature>
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        <location>
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    </feature>
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        <location>
            <begin position="560"/>
            <end position="573"/>
        </location>
    </feature>
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        <location>
            <begin position="988"/>
            <end position="1004"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017316046.1"/>
        </source>
    </evidence>
    <sequence length="1012" mass="112855" checksum="BC8D29C218400199" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QFU6</accession>
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        <submittedName>
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    </protein>
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        <name evidence="10 11 12 13 14" type="primary">zgc:92594</name>
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    <organism evidence="9 12">
        <name type="scientific">Ictalurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11 12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11 12">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017317177.1">
        <property type="nucleotide sequence ID" value="XM_017461688.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017317178.1">
        <property type="nucleotide sequence ID" value="XM_017461689.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017317179.1">
        <property type="nucleotide sequence ID" value="XM_017461690.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017317180.1">
        <property type="nucleotide sequence ID" value="XM_017461691.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017317182.1">
        <property type="nucleotide sequence ID" value="XM_017461693.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A2D0QFU6"/>
    <dbReference type="GeneID" id="108260947"/>
    <dbReference type="KEGG" id="ipu:108260947"/>
    <dbReference type="OrthoDB" id="342730at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0008270">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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    </dbReference>
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    <dbReference type="InterPro" id="IPR001841">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF73">
        <property type="entry name" value="E3 UBIQUITIN_ISG15 LIGASE TRIM25 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zf-B_box"/>
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    </dbReference>
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        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
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        <location>
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            <end position="59"/>
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    </feature>
    <feature type="domain" description="B box-type" evidence="8">
        <location>
            <begin position="160"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="376"/>
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    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="198"/>
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        <location>
            <begin position="380"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="434"/>
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        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017317177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017317178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017317179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017317180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017317182.1"/>
        </source>
    </evidence>
    <sequence length="444" mass="51827" checksum="55F9B106F19DE613" modified="2017-12-20" version="1">MAKALEDTSVLEEELTCPVCLDLYREPHLLPCGHNFCLQCLRRLKSRSERGRLRCPECRESHRCSKQWQKNFKLASIADGFRQRNRTQRPSQSRPGPSYRTAEVRCDYCGPDITGDSAKRYTAVKTCLKCEVSMCPEHVQHHLELPAFREHPLVEPLSDMRKRKCIEHDEMFRYYCMDERKFLCNACTIEGGHNRHAIKTLKNTMKDLRASLDTQLQKVNRKIRRVEKNVKEQREVEQQNKAFLEEVDKQVDTLRGVLMTRLDGFLSSLSEGVRAYEGENSLNIQQGVVQTEEDRNRLGVVHAGIEQLLQENDPFQFIKEYMSCAKSYRRLLKRPLFQPHAVTMDTEVIEECMEGKLEDFMTDLREHITTLINTVCKGDEVDEGDEGVEEEEEEDNSEENEDENDSSNEEEEQEEEMSNDEEDSDHSESTYDYTPEEEEEEEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QU66</accession>
    <name>A0A2D0QU66_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">small monomeric GTPase</fullName>
            <ecNumber evidence="4">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">rab27a</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017321859.1">
        <property type="nucleotide sequence ID" value="XM_017466370.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QU66"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000023636"/>
    <dbReference type="GeneID" id="108264641"/>
    <dbReference type="KEGG" id="ipu:108264641"/>
    <dbReference type="CTD" id="5873"/>
    <dbReference type="OMA" id="MHAYTED"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04127">
        <property type="entry name" value="Rab27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000402">
        <property type="entry name" value="Ras-related protein Rab-27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041837">
        <property type="entry name" value="Rab27a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0636">Prenylation</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017321859.1"/>
        </source>
    </evidence>
    <sequence length="222" mass="25229" checksum="51C6DD344C6222A7" modified="2017-12-20" version="1">MSDGDYDYLIKFLALGDSGVGKTSFLYQYTDGKFNSKFITTVGIDFREKRVMYKSSGPDGAAGRGQRIHVQLWDTAGQERFRSLTTAFFRDAMGFLLLFDLTNEQSFLNVRNWMSQLQMHAYCENPDIVLCGNKCDLEDQRFVKEEEARELASKYGIPYFETSAANGENVPQAVEVLLDLIMKRMERCVDRSWIPDGTMRSNGHSGSEMLEPPTQEKSKCAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R322</accession>
    <name>A0A2D0R322_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Nectin cell adhesion molecule 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">si:ch73-22o12.1</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Cell adhesion molecule that promotes cell-cell contacts and plays important roles in the development of the nervous system. Acts by forming homophilic or heterophilic trans-dimers.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Cis- and trans-homodimer. Can form trans-heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nectin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017324969.1">
        <property type="nucleotide sequence ID" value="XM_017469480.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R322"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008086"/>
    <dbReference type="GeneID" id="108266341"/>
    <dbReference type="KEGG" id="ipu:108266341"/>
    <dbReference type="OMA" id="REVTWLR"/>
    <dbReference type="OrthoDB" id="6413693at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000304">
        <property type="entry name" value="Nectin cell adhesion molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000298">
        <property type="entry name" value="Nectin cell adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
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</entry>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
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                <person name="Fu Q."/>
                <person name="Gao S."/>
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                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
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                <person name="Yuan Z."/>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
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                <person name="Dunham R."/>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
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                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
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                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017326407.2"/>
        </source>
    </evidence>
    <sequence length="1925" mass="214155" checksum="1A79427374CF369E" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R7L2</accession>
    <name>A0A2D0R7L2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Intraflagellar transport protein 88 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ift88</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017326041.1">
        <property type="nucleotide sequence ID" value="XM_017470552.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R7L2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005679"/>
    <dbReference type="GeneID" id="108266803"/>
    <dbReference type="KEGG" id="ipu:108266803"/>
    <dbReference type="CTD" id="8100"/>
    <dbReference type="OMA" id="RIKIMHN"/>
    <dbReference type="OrthoDB" id="1926212at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097546">
        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097730">
        <property type="term" value="C:non-motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060122">
        <property type="term" value="P:inner ear receptor cell stereocilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001822">
        <property type="term" value="P:kidney development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000468">
        <property type="entry name" value="Intraflagellar transport 88 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000106">
        <property type="entry name" value="Intraflagellar transport 88 homolog (Chlamydomonas)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44117">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 88 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44117:SF1">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 88 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="272"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="485"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="519"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="553"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="587"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="621"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="722"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="722"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="743"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="758"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="797"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="809"/>
            <end position="824"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017326041.1"/>
        </source>
    </evidence>
    <sequence length="824" mass="93545" checksum="C8D50E0449DC7FF8" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R8D9</accession>
    <name>A0A2D0R8D9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Golgi pH regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">si:ch73-390b10.2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>bromide(in) = bromide(out)</text>
            <dbReference type="Rhea" id="RHEA:75383"/>
            <dbReference type="ChEBI" id="CHEBI:15858"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>fluoride(in) = fluoride(out)</text>
            <dbReference type="Rhea" id="RHEA:76159"/>
            <dbReference type="ChEBI" id="CHEBI:17051"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>iodide(out) = iodide(in)</text>
            <dbReference type="Rhea" id="RHEA:66324"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Golgi pH regulator (TC 1.A.38) family.</text>
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    <dbReference type="RefSeq" id="XP_017326326.1">
        <property type="nucleotide sequence ID" value="XM_017470837.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R8D9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000397"/>
    <dbReference type="GeneID" id="108266990"/>
    <dbReference type="KEGG" id="ipu:108266990"/>
    <dbReference type="OMA" id="FSVYCVY"/>
    <dbReference type="OrthoDB" id="264392at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008308">
        <property type="term" value="F:voltage-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051452">
        <property type="term" value="P:intracellular pH reduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025969">
        <property type="entry name" value="ABA_GPCR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022535">
        <property type="entry name" value="Golgi_pH-regulator_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015672">
        <property type="entry name" value="GPHR/GTG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15948">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 89-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15948:SF0">
        <property type="entry name" value="GOLGI PH REGULATOR A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12430">
        <property type="entry name" value="ABA_GPCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12537">
        <property type="entry name" value="GPHR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="288"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="343"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="380"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="422"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Golgi pH regulator conserved" evidence="11">
        <location>
            <begin position="142"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Abscisic acid G-protein coupled receptor-like" evidence="10">
        <location>
            <begin position="277"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017326326.1"/>
        </source>
    </evidence>
    <sequence length="455" mass="53087" checksum="3512F3DF19CA6833" modified="2017-12-20" version="1">MSFFVDSVIMFTSQVLFFGFGWLFFMRQLFKDYEVRQYVIQVVFSVTFAFSCTMFELIIFEILGALSSTSRYFHWKLNLYVILLVLIFVVPFYIGYFVVSNIRLLQRQRLLFSCVVWFTFMYFFWKLGDPFPILSPKHGILSIEQLISRVGVIGVTLMALLSGFGAVNCPYTYMSYFLRNVTDSDILALERRLLQTMDMIVSKKKRIAMTRRQMYQRGEDQNKQTGFWGMLKSVTSSPSGSENLSLIQHEVDALEELSRQLFLETVDLQATKERIEYSKTFQGKYFNFLGYFFSIYCVWKIFMATINIVFDRVGKTDPVTRGIEITVNYLGIQFDVKFWSQHISFILVGIIIVTSIRGLLITLTKFFYAISSSKSSNVIVLVLAQIMGMYFVSSVLLMRMSMPLEYRTIVTEVLGELQFNFYHRWFDVIFLVSALSSILFLYLAHKQAPEKHMTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RA88</accession>
    <name>A0A2D0RA88_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peroxisomal biogenesis factor 19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Peroxin-19</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Peroxisomal farnesylated protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">pex19</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Necessary for early peroxisomal biogenesis. Acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Binds and stabilizes newly synthesized PMPs in the cytoplasm by interacting with their hydrophobic membrane-spanning domains, and targets them to the peroxisome membrane by binding to the integral membrane protein PEX3. Excludes CDKN2A from the nucleus and prevents its interaction with MDM2, which results in active degradation of TP53.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with a broad range of peroxisomal membrane proteins, including PEX3, PEX10, PEX11A, PEX11B, PEX12, PEX13, PEX14 and PEX16, PXMP2/PMP22, PXMP4/PMP24, SLC25A17/PMP34, ABCD1/ALDP, ABCD2/ALDRP, and ABCD3/PMP70. Also interacts with the tumor suppressor CDKN2A/p19ARF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxin-19 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017327016.2">
        <property type="nucleotide sequence ID" value="XM_017471527.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RA88"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020984"/>
    <dbReference type="GeneID" id="108267455"/>
    <dbReference type="KEGG" id="ipu:108267455"/>
    <dbReference type="CTD" id="5824"/>
    <dbReference type="OrthoDB" id="21292at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033328">
        <property type="term" value="F:peroxisome membrane targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045046">
        <property type="term" value="P:protein import into peroxisome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.900">
        <property type="entry name" value="Pex19, mPTS binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006708">
        <property type="entry name" value="Pex19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038322">
        <property type="entry name" value="Pex19_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12774">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12774:SF2">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04614">
        <property type="entry name" value="Pex19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="247"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017327016.2"/>
        </source>
    </evidence>
    <sequence length="282" mass="30795" checksum="289E10FCCB39B1BC" modified="2023-06-28" version="2">MASQSENSAAADAELDELLDSALDDFEKTNVPHAPPEAPSAEAKTGQKPPLLEDSQFFESLFEGEVAHQAREEWERAMAELAQEEPELIQQFHKLSEAAGKVGTDAVSQQEFASCLKDTLSGLAKNADNLQTTGLAGEDLAKTLEGLGLDENSEGGGDDGNILPIMQSIMQNLLSKDVLYPSLKEITEKYPEWLDSNRQSLSAEDVQRYEQQHRIMGEICSQFERDGDTESSFEKILEHMQKLQDLGHPPKELAGEAPPGLNFDPESLHLPGAAGGEQCSIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RBB6</accession>
    <name>A0A2D0RBB6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">receptor protein serine/threonine kinase</fullName>
            <ecNumber evidence="5">2.7.11.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">acvr1l</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27">SDA103</strain>
        </source>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="28">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[receptor-protein] + ATP = O-phospho-L-seryl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18673"/>
            <dbReference type="Rhea" id="RHEA-COMP:11022"/>
            <dbReference type="Rhea" id="RHEA-COMP:11023"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-threonyl-[receptor-protein] + ATP = O-phospho-L-threonyl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44880"/>
            <dbReference type="Rhea" id="RHEA-COMP:11024"/>
            <dbReference type="Rhea" id="RHEA-COMP:11025"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.30" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017327838.1">
        <property type="nucleotide sequence ID" value="XM_017472349.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RBB6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000011876"/>
    <dbReference type="GeneID" id="108267867"/>
    <dbReference type="KEGG" id="ipu:108267867"/>
    <dbReference type="CTD" id="30615"/>
    <dbReference type="OMA" id="HQAILCC"/>
    <dbReference type="OrthoDB" id="69842at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070724">
        <property type="term" value="C:BMP receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004675">
        <property type="term" value="F:transmembrane receptor protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14142">
        <property type="entry name" value="STKc_ACVR1_ALK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23535">
        <property type="entry name" value="TFP_LU_ECD_ALK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000018">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000064">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000472">
        <property type="entry name" value="Activin_recp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003605">
        <property type="entry name" value="GS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000333">
        <property type="entry name" value="TGFB_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255:SF96">
        <property type="entry name" value="RECEPTOR PROTEIN SERINE_THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR TYPE I AND II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01064">
        <property type="entry name" value="Activin_recp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08515">
        <property type="entry name" value="TGF_beta_GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00467">
        <property type="entry name" value="GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51256">
        <property type="entry name" value="GS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="19" id="KW-0464">Manganese</keyword>
    <keyword evidence="16 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17 28" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 24" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012767990" description="receptor protein serine/threonine kinase" evidence="24">
        <location>
            <begin position="22"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="GS" evidence="26">
        <location>
            <begin position="174"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="204"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_017327838.1"/>
        </source>
    </evidence>
    <sequence length="505" mass="56426" checksum="83A78557DF86C496" modified="2017-12-20" version="1">MGHRCTRVLFLVLLQVLRTLAEDGSIECVCVGRDCMAQQCKGDECYSSVTVRDGVPSFHRGCLIGPESRSLTCLTSPSASHAVECCARPMCNANVSVDSLIQLLLRNPEGEPMRYSVETLVLLVLVPFGVLVLLAMLAVPVCRRLHPRQLERLHEFDTEQGAIDGLIASNVGDSTLADLLDHSCTSGSGSGLPFLVQRTVARQISLVECVGKGRYGEVWRGQWQGENVAVKIFSSRDEKSWFRETEIYNTVLLRHENILGFMASDMTSRNSSTQLWLITHYHEHGSLYDYLQRTAVSAADALNMAASVASGLVHLHTEILGTEGKPAIAHRDLKSKNILVKKDMRCCIADLGLAVTHTQADNQLDVGNNPKVGTKRYMAPEVLDETIQMDCFDAYKRVDIWAFGLVLWEIARRTYSNGIVEDYKPPFYDLVPNDPSFEDMRKVVCVEQQRPFIPNRWFSDPTLSALVKLMKECWYQNPSARLTALRIKKTLDKILSSLEKGKPDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RDI3</accession>
    <name>A0A2D0RDI3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">tubb2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="11">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017328597.1">
        <property type="nucleotide sequence ID" value="XM_017473108.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RDI3"/>
    <dbReference type="GeneID" id="108268242"/>
    <dbReference type="KEGG" id="ipu:108268242"/>
    <dbReference type="CTD" id="554127"/>
    <dbReference type="OrthoDB" id="1662883at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000003">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013838">
        <property type="entry name" value="Beta-tubulin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="13">
        <location>
            <begin position="47"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="14">
        <location>
            <begin position="246"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="425"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="429"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="446"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017328597.1"/>
        </source>
    </evidence>
    <sequence length="457" mass="51499" checksum="E465ADA524DD09E1" modified="2017-12-20" version="1">MREIVHIQAGQCGNQIGAKFWEVISDEHGIDYSGTYNGDSKLQLERINVYYNEASGGKFVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDAVLDVVRKESENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMTTFSVTPSPKVSDTVVEPYNATLSIHQLVENTDETFSIDNEALYDICFRTLKLTTPTYGDLNHLVSAAMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMTACDPRHGRYLTVAAIFRGRMSMKEVDEQMLSVQNRNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFRRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLISEYQQYQDATAEEAEEYEEDEMEEEEDIRHDGRQEGHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RDX3</accession>
    <name>A0A2D0RDX3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">Thialysine N-epsilon-acetyltransferase isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">sat2a.1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6 7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017328728.1">
        <property type="nucleotide sequence ID" value="XM_017473239.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053537350.1">
        <property type="nucleotide sequence ID" value="XM_053681375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RDX3"/>
    <dbReference type="GeneID" id="108268333"/>
    <dbReference type="CTD" id="569184"/>
    <dbReference type="GeneTree" id="ENSGT00950000183121"/>
    <dbReference type="OrthoDB" id="7305308at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000064">
        <property type="entry name" value="GNAT family acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051016">
        <property type="entry name" value="Diverse_Substrate_AcTransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10545">
        <property type="entry name" value="DIAMINE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10545:SF66">
        <property type="entry name" value="DIAMINE N-ACETYLTRANSFERASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="4">
        <location>
            <begin position="24"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017328728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053537350.1"/>
        </source>
    </evidence>
    <sequence length="171" mass="19480" checksum="42E318983AA27B02" modified="2017-12-20" version="1">MDFATRAAKLEDCKDIMRMMSELTEYEKITDQVTITQKDIEQDGFSQNPFFHAIIAEVPEQHKTKDGHTKIGYSVYSFTYSTWKGRDVYMVDLYVMPEFRGKGIGKALMSKVAQLGQAAGCTNLSFTVVNWNKTALDFYRKQGCVDLTSDLGYHCMSCNGDVFQRMANRDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RGQ8</accession>
    <name>A0A2D0RGQ8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Golgi-associated kinase 1B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">gask1b</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GASK family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017329316.1">
        <property type="nucleotide sequence ID" value="XM_017473827.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RGQ8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000005612"/>
    <dbReference type="GeneID" id="108268710"/>
    <dbReference type="KEGG" id="ipu:108268710"/>
    <dbReference type="CTD" id="51313"/>
    <dbReference type="OMA" id="PPWFSAQ"/>
    <dbReference type="OrthoDB" id="10011371at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029207">
        <property type="entry name" value="FAM198"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15905:SF1">
        <property type="entry name" value="GOLGI-ASSOCIATED KINASE 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15905">
        <property type="entry name" value="GOLGI-ASSOCIATED KINASE 1B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15051">
        <property type="entry name" value="FAM198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017329316.1"/>
        </source>
    </evidence>
    <sequence length="479" mass="55647" checksum="5E7F8D3A141DB4A4" modified="2017-12-20" version="1">MEKHLIRPGRSARTLLRLTCLSFWRVLRSCWRSSRSRRALLLAFACLLYLFCLELWIDSGDRDRNLTEGRRWILERIPGSTSPTRSNVVYITLKSKRHKPAILRATLRPKSRRKKVRNVENQSRDAARHRGEETLKRIESTDLWRKVLHSLYKPGQLDDDDGSSIRIYSQKSPPWFSQADIETMRFLSKCKIGRVEVLEPENAPPALLFKSVSGFNRSSDHAGECDGRCGVIKGPEDMSEVFAFHLDRVLRLNRSSVAISRRFRALDGQLCPVTLYDPSVVPVPESRSGSLRWSSYQTSLQYRCWQHGVPKPEWSCSSLHHHEWSRVTLFDFLLQNHQRFDRNCCGFRPRPQDSCVQLGHHRECGNVDNLELSHIVHRKNDPRRLVYVNNNAFFDRDEENLDFKLLEGIKELPEVAVSVLRSQRLRQNLLQSLFVDEQFWENQGGRRGIDKLIDVIEGRARVLLRYINAHGLNVVAMNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RIL4</accession>
    <name>A0A2D0RIL4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Transcription factor COE1-A isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ebf1a</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="11">Forms either a homodimer or a heterodimer with a related family member.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the COE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017330357.1">
        <property type="nucleotide sequence ID" value="XM_017474868.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RIL4"/>
    <dbReference type="GeneID" id="108269197"/>
    <dbReference type="KEGG" id="ipu:108269197"/>
    <dbReference type="CTD" id="565310"/>
    <dbReference type="OrthoDB" id="25246at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11606">
        <property type="entry name" value="COE_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01175">
        <property type="entry name" value="IPT_COE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4280:FF:000001">
        <property type="entry name" value="transcription factor COE1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.3180:FF:000001">
        <property type="entry name" value="transcription factor COE1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001696">
        <property type="entry name" value="Transcription factor COE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3180">
        <property type="entry name" value="Transcription factor COE1, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032200">
        <property type="entry name" value="COE_DBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038173">
        <property type="entry name" value="COE_DBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032201">
        <property type="entry name" value="COE_HLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038006">
        <property type="entry name" value="COE_IPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003523">
        <property type="entry name" value="Transcription_factor_COE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018350">
        <property type="entry name" value="Transcription_factor_COE_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10747">
        <property type="entry name" value="TRANSCRIPTION FACTOR COE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16422">
        <property type="entry name" value="COE1_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16423">
        <property type="entry name" value="COE1_HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01345">
        <property type="entry name" value="COE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="12" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="IPT/TIG" evidence="14">
        <location>
            <begin position="261"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="453"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="453"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="471"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017330357.1"/>
        </source>
    </evidence>
    <sequence length="604" mass="65769" checksum="6474C99B3C65EE06" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RMP4</accession>
    <name>A0A2D0RMP4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Fibroblast growth factor-binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fgfbp3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fibroblast growth factor-binding protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017331210.1">
        <property type="nucleotide sequence ID" value="XM_017475721.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RMP4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026119"/>
    <dbReference type="GeneID" id="108269744"/>
    <dbReference type="KEGG" id="ipu:108269744"/>
    <dbReference type="CTD" id="143282"/>
    <dbReference type="OrthoDB" id="8803710at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019838">
        <property type="term" value="F:growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010510">
        <property type="entry name" value="FGF1-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15258">
        <property type="entry name" value="FGF BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15258:SF3">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06473">
        <property type="entry name" value="FGF-BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0340">Growth factor binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017331210.1"/>
        </source>
    </evidence>
    <sequence length="184" mass="21108" checksum="1024A5B8FC503AA8" modified="2017-12-20" version="1">MPRLMKLFFLVLLVLFVFVSPSEAKKKTKQKSGEDKILSSGDLSTKNGDRCTWQTREGKNNMVLQIKCSTHSEEAVLESTNYECEFKGKPNECPAYVDKPTQYWKQVLGKLKRQSSCKGIKVLKANMCKNAPNTSHMKLVRDKEEKKDEELMMRDEGMSDGEAETESYCAEGWDPFCHFFTKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RQT0</accession>
    <name>A0A2D0RQT0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Tetranectin-like protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">clec3a</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017332854.1">
        <property type="nucleotide sequence ID" value="XM_017477365.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RQT0"/>
    <dbReference type="GeneID" id="108270550"/>
    <dbReference type="KEGG" id="ipu:108270550"/>
    <dbReference type="CTD" id="10143"/>
    <dbReference type="OrthoDB" id="10032136at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="CDD" id="cd03596">
        <property type="entry name" value="CLECT_tetranectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000010">
        <property type="entry name" value="C-type lectin domain family 3 member A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051663">
        <property type="entry name" value="CLec_Tetranectin-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22799:SF2">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 3 MEMBER A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22799">
        <property type="entry name" value="TETRANECTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012790797" evidence="3">
        <location>
            <begin position="23"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="69"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017332854.1"/>
        </source>
    </evidence>
    <sequence length="193" mass="21877" checksum="D891A8D7F19C8BBF" modified="2017-12-20" version="1">MAFPRSFLLCLLSCTLLHQGCARPSRSRKAAAGQEDDDTKAQIDKLWQEVNSLKEMQALQTVCLRGIKAHKKCYLVVEEPKHYHEANEDCIAQGGTLATPRNPRENSDLRDYARLSSPGTKDVWIGVTDIVKEGQYVDVNSMPITYFNWDRAKKEPTGGKRESCAVLSLSAQGKWHDEVCRTEKRYICEYLIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RRD8</accession>
    <name>A0A2D0RRD8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">G protein-coupled receptor 137Ba</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">gpr137bb</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017333069.1">
        <property type="nucleotide sequence ID" value="XM_017477580.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RRD8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032788"/>
    <dbReference type="GeneID" id="108270727"/>
    <dbReference type="KEGG" id="ipu:108270727"/>
    <dbReference type="CTD" id="101884293"/>
    <dbReference type="OMA" id="TANTFGP"/>
    <dbReference type="OrthoDB" id="192544at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045779">
        <property type="term" value="P:negative regulation of bone resorption"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045671">
        <property type="term" value="P:negative regulation of osteoclast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21476">
        <property type="entry name" value="7tm_GPR137B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029723">
        <property type="entry name" value="GPR137"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15146:SF6">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 137BB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15146">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN GPR137"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0458">Lysosome</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="287"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017333069.1"/>
        </source>
    </evidence>
    <sequence length="391" mass="44277" checksum="6983C844E22B2862" modified="2017-12-20" version="1">MEDTRLMEERREDGSRASGANASLSPATLSPAVPPYVKLGLTVAYTAFYSLLFAFVYAQLWLVLRYRHKRFSYKTVFLFLCLLWAALRALLFSFYFRDCATANTLGPFAFWLLYCFPVCLQFFTLSLMNLYCAQVFFKAKSKYSPHLLKYKFPLYLVFVGASLLFLLVNLACALLVKMTDAQVKTIVLVRVTINDSLFVLCAISLSVCLYKVAKMSLASIYLESKGTSVCQVTLIGIMVILLYTSRACYNLVVLALTDIENINSFDYDWYNVSDQADLRSNLGDAGYVVFGVILFVWELLPTSLVVFFFRVRKPAQDRSASAIPSHVFSTKRYFFDNPRRYDSDDDLAWGSYPQNSSTSLSSDCYDWGSRSSSFLINLGGENQRSSPGLNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RS92</accession>
    <name>A0A2D0RS92_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">CTD small phosphatase-like protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ctdspl3</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017332825.1">
        <property type="nucleotide sequence ID" value="XM_017477336.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RS92"/>
    <dbReference type="GeneID" id="108270532"/>
    <dbReference type="KEGG" id="ipu:108270532"/>
    <dbReference type="CTD" id="571337"/>
    <dbReference type="OrthoDB" id="277011at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000093">
        <property type="entry name" value="NLI interacting factor-like phosphatase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="3">
        <location>
            <begin position="187"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017332825.1"/>
        </source>
    </evidence>
    <sequence length="370" mass="42884" checksum="E0D19EC996F21FA8" modified="2017-12-20" version="1">MRLRSRIISLESPSSQQPSTPRRRRRPDLSRASRIEQNQSPSPDVQVLKDCSNLFSDPVPTAVKRPRLRHGRKRVLPLQACKAESDFKTPVQHLRETQYSSVNPAARSLYSPVVRFVTPTKDNERTRRGAVFSPEQCVFGYSAISSLSEDEENDEVFSPFTFIKNIPNQSQQSRAASTLRDIPPKTRSTPAATMVLDLDETLVFSSLNRLEDAEYTFNTIFQDQEYTVYMVLRPHVNEFLQAMVKHFEMFVYTSAKKEYAEMMVDILDPRKKIFRHRLYQDDCACVLGHYIKDLEVLERDLSKTVILDNAPHTYPYHLTNAIPIKSWCGDKEDRELQRLIPYLGKLAQANDFRTVLKRRTDHFHNLLTED</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RSN6</accession>
    <name>A0A2D0RSN6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cell division control protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">cdc6</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14 16">Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with PCNA, ORC1, cyclin-CDK. Interacts with HUWE1. Interacts with ANKRD17. Interacts with GRWD1; origin binding of GRWD1 is dependent on CDC6. Interacts with CDT1; are mutually dependent on one another for loading MCM complexes onto chromatin. Interacts with TTC4. Interacts (via Cy motif) with CCNF; the interaction takes place during G2 and M phase. Interacts with CDH1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the CDC6/cdc18 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017333181.1">
        <property type="nucleotide sequence ID" value="XM_017477692.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RSN6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018180"/>
    <dbReference type="GeneID" id="108270781"/>
    <dbReference type="KEGG" id="ipu:108270781"/>
    <dbReference type="CTD" id="990"/>
    <dbReference type="OMA" id="WPTDEVY"/>
    <dbReference type="OrthoDB" id="1926878at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08768">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000265">
        <property type="entry name" value="Cell division control protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000058">
        <property type="entry name" value="Cell division control protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000547">
        <property type="entry name" value="Cell division control protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016314">
        <property type="entry name" value="Cdc6/18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015163">
        <property type="entry name" value="Cdc6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054425">
        <property type="entry name" value="Cdc6_ORC1-like_ATPase_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF26">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22606">
        <property type="entry name" value="Cdc6-ORC-like_ATPase_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09079">
        <property type="entry name" value="WHD_Cdc6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001767">
        <property type="entry name" value="Cdc6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01074">
        <property type="entry name" value="Cdc6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6 21" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="18">
        <location>
            <begin position="230"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc6 C-terminal" evidence="19">
        <location>
            <begin position="492"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="91"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="432"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="122"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="144"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="432"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00062730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR001767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM01074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017333181.1"/>
        </source>
    </evidence>
    <sequence length="587" mass="64032" checksum="A19BF78FD921014A" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RY90</accession>
    <name>A0A2D0RY90_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Dynactin subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">dctn2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynactin subunit 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017335467.1">
        <property type="nucleotide sequence ID" value="XM_017479978.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RY90"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000036460"/>
    <dbReference type="GeneID" id="108271974"/>
    <dbReference type="KEGG" id="ipu:108271974"/>
    <dbReference type="CTD" id="10540"/>
    <dbReference type="OMA" id="YKFGDWE"/>
    <dbReference type="OrthoDB" id="4977at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005869">
        <property type="term" value="C:dynactin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028133">
        <property type="entry name" value="Dynamitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15346">
        <property type="entry name" value="DYNACTIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04912">
        <property type="entry name" value="Dynamitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0243">Dynein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="96"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="219"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017335467.1"/>
        </source>
    </evidence>
    <sequence length="403" mass="44288" checksum="113DA080C5403FBE" modified="2017-12-20" version="1">MADPKYANLPGIASNEPDVYETSDLPEDDQAQFESEELCSDSVERIVVNPNAAYDKFKDKRVGTKGLDFSDRISKSRRVGYESGDYELLGEGCGVKETPQQKYQRLVNEIQELTEEVEHIQTSTKESSAEERLTPVVLAQQAAQLKQQLVSAHLDSLLGPNAHINLADPDGALAKRLMTQLEVARGVRSTAGGEGKSAAPKGPDGVVLYELHSRPEHEKFTESAKIAELERRLADLETAVGSGLDKQGPLSAGLQGASLVETMELLQARVSALDAATLDQVEARLQSVLGKMNEIAKHKAAIEDAETQNKVTQLYDVVQKWDAMATSLPQVVQRLIAVKELHEQAMQFGQLLAHLDTTQQMINNSLKDNSTLLTQVQQTMKENLVSVEENFAALDQRMKKLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S4U8</accession>
    <name>A0A2D0S4U8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10">Stromal membrane-associated protein 2 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">smap2</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017337777.1">
        <property type="nucleotide sequence ID" value="XM_017482288.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017337778.1">
        <property type="nucleotide sequence ID" value="XM_017482289.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S4U8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019739"/>
    <dbReference type="GeneID" id="108273193"/>
    <dbReference type="KEGG" id="ipu:108273193"/>
    <dbReference type="CTD" id="64744"/>
    <dbReference type="OrthoDB" id="10266696at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08859">
        <property type="entry name" value="ArfGap_SMAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000009">
        <property type="entry name" value="stromal membrane-associated protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051718">
        <property type="entry name" value="ARF_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705">
        <property type="entry name" value="FI20236P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705:SF4">
        <property type="entry name" value="STROMAL MEMBRANE-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="7">
        <location>
            <begin position="13"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="210"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="395"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="214"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="249"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="397"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017337777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017337778.1"/>
        </source>
    </evidence>
    <sequence length="433" mass="47562" checksum="C096FDF0CC700979" modified="2017-12-20" version="1">MTGKSVKDVDRYQAVLSSLLSLDENKFCADCRAKGPRWASWNLGIFICIRCAGIHRNLGVHISRVKSVNLDQWTQEQILCMQEMGNAKARRLYEAFLPECFQRPETDQSAEMFIRDKYEKKKYMDKCIDIQTFRKEKSETVTKEPPVVFEKMKLKKEEPQHYKSSPKKQSQSVNDLLGLDTPVASAPVANGSASPEQSVALDLFSSLPTATSHSARSTPGSSSVSSSMPKGRAAASVPENLSLFLDPPSKNEESSKKLSKDSILSLYSSVTPQTNMTANAGMYMGAAQMGYAPAPAYGHYQALATQQGMMGTMMAPQVNMLGQPGVMAPTGLAAPSPYTVGVQGGMMGVQGGMMGGVGALPQQAYTMQQTQQLQWNITQPDLLYKMNQHHLTNRNQDFNKHCDDSPHGGYERSRPEPHDGIRTAHGRSLSSQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S549</accession>
    <name>A0A2D0S549_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC108272957</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017337340.1">
        <property type="nucleotide sequence ID" value="XM_017481851.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S549"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000017185"/>
    <dbReference type="GeneID" id="108272957"/>
    <dbReference type="KEGG" id="ipu:108272957"/>
    <dbReference type="OMA" id="CTHTHKE"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23793">
        <property type="entry name" value="UBCc_UBE2E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000003">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 E3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="10">
        <location>
            <begin position="64"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="7">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017337340.1"/>
        </source>
    </evidence>
    <sequence length="210" mass="23335" checksum="2EB68E377215C5F0" modified="2017-12-20" version="1">MSSEVQPRPDDSPNTSGGSSDAEQRDVAPSVQPEEREREREREQAPPKKKEAKFSSKTPAKLSTSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDIAFTPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIAKQWTKRYAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SAS5</accession>
    <name>A0A2D0SAS5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Histone acetyltransferase</fullName>
            <ecNumber evidence="25">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="primary">kat7b</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">SDA103</strain>
        </source>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="29">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="30 25">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 25">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 25">Belongs to the MYST (SAS/MOZ) family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="25"/>
    <dbReference type="RefSeq" id="XP_017339832.1">
        <property type="nucleotide sequence ID" value="XM_017484343.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SAS5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000015847"/>
    <dbReference type="GeneID" id="108274269"/>
    <dbReference type="KEGG" id="ipu:108274269"/>
    <dbReference type="CTD" id="323181"/>
    <dbReference type="OrthoDB" id="787137at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036409">
        <property type="term" value="C:histone H3-K14 acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036408">
        <property type="term" value="F:histone H3K14 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010485">
        <property type="term" value="F:histone H4 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902035">
        <property type="term" value="P:positive regulation of hematopoietic stem cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045935">
        <property type="term" value="P:positive regulation of nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000092">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.60:FF:000001">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000001">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.320.30:FF:000002">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.60">
        <property type="entry name" value="N-acetyl transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002717">
        <property type="entry name" value="HAT_MYST-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050603">
        <property type="entry name" value="MYST_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040706">
        <property type="entry name" value="Zf-MYST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002515">
        <property type="entry name" value="Znf_C2H2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036060">
        <property type="entry name" value="Znf_C2H2C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615:SF161">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE KAT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01853">
        <property type="entry name" value="MOZ_SAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01530">
        <property type="entry name" value="zf-C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17772">
        <property type="entry name" value="zf-MYST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103637">
        <property type="entry name" value="CCHHC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51726">
        <property type="entry name" value="MYST_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51802">
        <property type="entry name" value="ZF_CCHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0007">Acetylation</keyword>
    <keyword evidence="21" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="22" id="KW-0137">Centromere</keyword>
    <keyword evidence="15" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="19" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20 25" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0804">Transcription</keyword>
    <keyword evidence="17" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="12 24" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYST-type HAT" evidence="27">
        <location>
            <begin position="273"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="166"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="26">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="200"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="23">
        <location>
            <position position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR602717-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU361211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_017339832.1"/>
        </source>
    </evidence>
    <sequence length="558" mass="64169" checksum="8396976A46021322" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SBI9</accession>
    <name>A0A2D0SBI9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Fibroblast growth factor 18a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fgf18a</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017340092.1">
        <property type="nucleotide sequence ID" value="XM_017484603.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SBI9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012803"/>
    <dbReference type="GeneID" id="108274431"/>
    <dbReference type="KEGG" id="ipu:108274431"/>
    <dbReference type="CTD" id="359829"/>
    <dbReference type="OMA" id="XPHSAMS"/>
    <dbReference type="OrthoDB" id="5988014at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007498">
        <property type="term" value="P:mesoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042664">
        <property type="term" value="P:negative regulation of endodermal cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000007">
        <property type="entry name" value="Fibroblast growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00442">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0339">Growth factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012745388" evidence="6">
        <location>
            <begin position="21"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017340092.1"/>
        </source>
    </evidence>
    <sequence length="208" mass="24246" checksum="2D871D407D6568CC" modified="2017-12-20" version="1">MRSFLSTLAVLYIQMMLVMCNPLQVFGVDGVNFSMHVENQTRARDPMSRRQPRVYQLYSRTSCKHVQVLGRRISARGEDGDKFAQLVVEADTFGSQVRIRGKETNYYLCMNRRGKLVGKKASNRSEDCVFIEMVLENNYTALMSARYKDWYVGFTKRGRPRRGPQTLLNQQDVHFMKRLPPGEQPDHMPFRFTTVSKRSKRVRAARPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SBJ9</accession>
    <name>A0A2D0SBJ9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Complement component C1q receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">thbd</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017339610.1">
        <property type="nucleotide sequence ID" value="XM_017484121.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SBJ9"/>
    <dbReference type="GeneID" id="108274163"/>
    <dbReference type="KEGG" id="ipu:108274163"/>
    <dbReference type="CTD" id="7056"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050772">
        <property type="term" value="P:positive regulation of axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000009">
        <property type="entry name" value="Low-density lipoprotein receptor isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051505">
        <property type="entry name" value="C-type_lectin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057350">
        <property type="entry name" value="THBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14789">
        <property type="entry name" value="CHONDROLECTIN VARIANT CHODLFDELTAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14789:SF9">
        <property type="entry name" value="THROMBOMODULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25444">
        <property type="entry name" value="THBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001775">
        <property type="entry name" value="CD93/CD141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00907">
        <property type="entry name" value="THRMBOMODULN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 14" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0430">Lectin</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12 20" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 16" id="KW-0732">Signal</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012045091" evidence="16">
        <location>
            <begin position="19"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="477"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="18">
        <location>
            <begin position="27"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="288"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="17">
        <location>
            <begin position="329"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017339610.1"/>
        </source>
    </evidence>
    <sequence length="539" mass="59711" checksum="51A0CA78350CB2D2" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SI82</accession>
    <name>A0A2D0SI82_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Paraneoplastic antigen Ma1 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC108275597</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017341990.1">
        <property type="nucleotide sequence ID" value="XM_017486501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SI82"/>
    <dbReference type="GeneID" id="108275597"/>
    <dbReference type="KEGG" id="ipu:108275597"/>
    <dbReference type="OrthoDB" id="115435at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026523">
        <property type="entry name" value="PNMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048270">
        <property type="entry name" value="PNMA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048271">
        <property type="entry name" value="PNMA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23095">
        <property type="entry name" value="PARANEOPLASTIC ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23095:SF51">
        <property type="entry name" value="PARANEOPLASTIC ANTIGEN MA1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14893">
        <property type="entry name" value="PNMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20846">
        <property type="entry name" value="PNMA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Paraneoplastic antigen Ma-like N-terminal" evidence="2">
        <location>
            <begin position="12"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Paraneoplastic antigen Ma-like C-terminal" evidence="1">
        <location>
            <begin position="160"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017341990.1"/>
        </source>
    </evidence>
    <sequence length="340" mass="38424" checksum="3ECD6106639218C6" modified="2017-12-20" version="1">MELQSVKLELVKELREWCQGESLDQIHTLLAVVPKEFEVAQIGETLETIKALGRVRVRGREFNTTLDRLTVLCECKEKIDPTKVPYEVMHTHTDEAWPIVMATEAPALDEDAQTPLKVLLEASTSSGSAESIIRAVGDLLSKIEKPSGENSNYRHLRMLSGTLPAPAEEEVLEHWIEQARLMVEESDCSAKEKRQRIMESLRRPALAVVKTVCTADADVTPHKCLDAIESAFRTAESGEYLYFDFRLLYQGQYEKLSDLLRRLEQSLTKVVIKGGVPASHVDIARVEQLLWGAIHSDLIIVQLKLRERKQNPPTFLELLAEIRAERSSPPLESRLICLHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SIK9</accession>
    <name>A0A2D0SIK9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">(Lyso)-N-acylphosphatidylethanolamine lipase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Alpha/beta hydrolase domain-containing protein 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Alpha/beta-hydrolase 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC108275917</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1-O-(1Z-octadecenoyl)-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-N-hexadecanoyl-ethanolamine + H2O = 1-O-(1Z-octadecenyl)-sn-glycero-3-phospho-N-hexadecanoyl-ethanolamine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55240"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:137009"/>
            <dbReference type="ChEBI" id="CHEBI:138663"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:55241"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(N-hexadecanoyl)-serine + H2O = 1-octadecanoyl-2-hydroxy-sn-glycero-3-phospho-(N-hexadecanoyl)-serine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55236"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:138661"/>
            <dbReference type="ChEBI" id="CHEBI:138662"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:55237"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N,1-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45396"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:85222"/>
            <dbReference type="ChEBI" id="CHEBI:85229"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:45397"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N,1-diacyl-sn-glycero-3-phosphoethanolamine + H2O = N-acyl-sn-glycero-3-phosphoethanolamine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45420"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:85216"/>
            <dbReference type="ChEBI" id="CHEBI:85225"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:45421"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45400"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:85223"/>
            <dbReference type="ChEBI" id="CHEBI:85230"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:45401"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N-eicosanoyl-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-eicosanoyl-sn-glycero-3-phosphoethanolamine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45392"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:85221"/>
            <dbReference type="ChEBI" id="CHEBI:85228"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:45393"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N-hexadecanoyl-1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-hexadecanoyl-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45424"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:78097"/>
            <dbReference type="ChEBI" id="CHEBI:85217"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:45425"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N-hexadecanoyl-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-hexadecanoyl-sn-glycero-3-phosphoethanolamine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45384"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:85217"/>
            <dbReference type="ChEBI" id="CHEBI:85226"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:45385"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N-octadecanoyl-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-octadecanoyl-sn-glycero-3-phospho-ethanolamine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45388"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:85219"/>
            <dbReference type="ChEBI" id="CHEBI:85227"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:45389"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>an N-acyl-1,2-diacyl-sn-glycero-3-phosphoethanolamine + H2O = N,1-diacyl-sn-glycero-3-phosphoethanolamine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45460"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:62537"/>
            <dbReference type="ChEBI" id="CHEBI:85216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:45461"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017342539.1">
        <property type="nucleotide sequence ID" value="XM_017487050.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SIK9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000002324"/>
    <dbReference type="GeneID" id="108275917"/>
    <dbReference type="KEGG" id="ipu:108275917"/>
    <dbReference type="OMA" id="RVQNDLW"/>
    <dbReference type="OrthoDB" id="7457040at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042171">
        <property type="term" value="F:lysophosphatidic acid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000044">
        <property type="entry name" value="Abhydrolase domain containing 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF21">
        <property type="entry name" value="(LYSO)-N-ACYLPHOSPHATIDYLETHANOLAMINE LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="19">
        <location>
            <begin position="77"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00050487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00050883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00054321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00068782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00076451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00081215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017342539.1"/>
        </source>
    </evidence>
    <sequence length="349" mass="39486" checksum="1F3A317A04FD80BB" modified="2017-12-20" version="1">MHDETESDEKLSRSAWWTSWRPTSLSLLKNTETKILDRVQNDLWARFVSLPTQDRIWTLTVTHKSRRDAPKSCSRTPLVMVHGFGGGVGLWIRNLDPLCRSRPVFAFDLLGFGRSSRPRFPLDPAQAEQQSVISIEHWRQALGLEKMILLGHSLGGYLATSYAIQYPERVRHLILVDPWGFKPVPPEGVASLGCPRWVEIIIAMASFFNPLAVVRATGPWGPKLISRVRPDFKRNFEELFQDDTIMEYIYHCNAQSPSGEVGFRAMSVSLLWAKRPMLDRVHLLPPDLPVTLVYGGQSWMDSATGEQVARLRPNSYTHTMVIEGAPHHVYADKPDAFNAVVQSICDTVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SIL7</accession>
    <name>A0A2D0SIL7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein si:ch211-149b19.4 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">si:ch211-149b19.4</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017342336.2">
        <property type="nucleotide sequence ID" value="XM_017486847.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SIL7"/>
    <dbReference type="GeneID" id="108275830"/>
    <dbReference type="KEGG" id="ipu:108275830"/>
    <dbReference type="OrthoDB" id="8933418at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039887569" evidence="2">
        <location>
            <begin position="19"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="266"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017342336.2"/>
        </source>
    </evidence>
    <sequence length="297" mass="31051" checksum="EE13008589A73F83" modified="2023-06-28" version="2">MEMQLAVLLLWVLRITDGFPVSSQLSLAPSQSSPSYTLLVPVRVLGLNGPQVVLVPVSTDWSTQRNQNPTAQSEQTHTLTEPEQKQMTGQNQFVNSPFLQLPTVSPDTPAPENPLPQGQVLPVWNTIPGVVPLQPGVNGQAFIPVWAPPAGGAARGQRQVVSVAQAAVQSNSASSEEGDAQVIYILPVSSEIQNMGIMEGGQGGVDPELTPDPNPNANLHLKPDTNPNPDPKPGHLQLHNTPVPSPTLPAGVQEITDSLGKAPPDAGAGQRTQTGGATTSCNHNGGVANANVNTPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0STZ9</accession>
    <name>A0A2D0STZ9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">26S proteasome non-ATPase regulatory subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">psmd2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Binds to the intracellular domain of tumor necrosis factor type 1 receptor. The binding domain of TRAP1 and TRAP2 resides outside the death domain of TNFR1.</text>
    </comment>
    <comment type="function">
        <text evidence="1 8">Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases and few additional components including PSMD2. Interacts with RPGRIP1L. Interacts with CRY1 in a KDM8-dependent manner. Interacts (via C-terminus) with phosphatase UBLCP1 (via ubiquitin-like domain); the interaction recruits UBLCP1 to the 19S regulatory particle where it dephosphorylates 19S subunit PSMC2/RPT1 which impairs PSMC2 ATPase activity and disrupts 26S proteasome assembly.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the proteasome subunit S2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017345991.1">
        <property type="nucleotide sequence ID" value="XM_017490502.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0STZ9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000027834"/>
    <dbReference type="GeneID" id="108277688"/>
    <dbReference type="KEGG" id="ipu:108277688"/>
    <dbReference type="CTD" id="5708"/>
    <dbReference type="OrthoDB" id="10252509at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000026">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016643">
        <property type="entry name" value="26S_Psome_Rpn1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002015">
        <property type="entry name" value="Proteasome/cyclosome_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041433">
        <property type="entry name" value="RPN1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040892">
        <property type="entry name" value="RPN1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01851">
        <property type="entry name" value="PC_rep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18051">
        <property type="entry name" value="RPN1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17781">
        <property type="entry name" value="RPN1_RPN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015965">
        <property type="entry name" value="26S_Psome_Rpn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 13" id="KW-0647">Proteasome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RPN1 N-terminal" evidence="10">
        <location>
            <begin position="46"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="26S proteasome non-ATPase regulatory subunit RPN1 C-terminal" evidence="11">
        <location>
            <begin position="843"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="616"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="616"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR015965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017345991.1"/>
        </source>
    </evidence>
    <sequence length="898" mass="99744" checksum="C382F3AFA4F8A606" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SVI3</accession>
    <name>A0A2D0SVI3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Transmembrane protein 59</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">tmem59</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Acts as a regulator of autophagy in response to S.aureus infection by promoting activation of LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C). Acts by interacting with ATG16L1, leading to promote a functional complex between LC3 and ATG16L1 and promoting LC3 lipidation and subsequent activation of autophagy. Modulates the O-glycosylation and complex N-glycosylation steps occurring during the Golgi maturation of several proteins such as APP, BACE1, SEAP or PRNP. Inhibits APP transport to the cell surface and further shedding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with ATG16L1 (via WD repeats).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Late endosome membrane</location>
            <topology evidence="15">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TMEM59 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017346690.1">
        <property type="nucleotide sequence ID" value="XM_017491201.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SVI3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000011620"/>
    <dbReference type="GeneID" id="108278060"/>
    <dbReference type="KEGG" id="ipu:108278060"/>
    <dbReference type="CTD" id="9528"/>
    <dbReference type="OMA" id="MGCHNQL"/>
    <dbReference type="OrthoDB" id="6371519at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022065">
        <property type="entry name" value="Uncharacterised_TMEM59"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28652:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28652">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 59-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12280">
        <property type="entry name" value="BSMAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14" id="KW-0458">Lysosome</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00038589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00039377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00045285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017346690.1"/>
        </source>
    </evidence>
    <sequence length="323" mass="36676" checksum="82DD35EE8235EF58" modified="2017-12-20" version="1">MKCVWLFLTFLLSVFLCCSASAFDGVLGSTASCHKTCRMTYSLHTYPREVELFACQRGCRLFSICQFVGDSENLNQTKSECDSACNEAYDEVDEEFACSLGCQNQLPFAEQRQEQLLAMMPRIHLLYPLTLVRGFWEDMMNQAHEFIASSWTFYLQADDGKVIVFQTGPQVQFVSQFDLQGEELKEEPQSSDPEINEPLYREYSRSFGQERFGVVAQDQSSSQDDYNFFSCLSRSSWLPGWILTTTLVLSVLVLIWICCATMATAADQYVPAEKLSIYGDMEYMKEQNLKPYPQSSLLIVSSLVKEGEAEALPHKLKVDLTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SVP6</accession>
    <name>A0A2D0SVP6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Synaptotagmin-like protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">sytl2b</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017346601.1">
        <property type="nucleotide sequence ID" value="XM_017491112.3"/>
    </dbReference>
    <dbReference type="GeneID" id="108278002"/>
    <dbReference type="KEGG" id="ipu:108278002"/>
    <dbReference type="CTD" id="572466"/>
    <dbReference type="OrthoDB" id="195679at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070382">
        <property type="term" value="C:exocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042043">
        <property type="term" value="F:neurexin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04020">
        <property type="entry name" value="C2B_SLP_1-2-3-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000006">
        <property type="entry name" value="Synaptotagmin-like 5, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000040">
        <property type="entry name" value="synaptotagmin-like protein 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010911">
        <property type="entry name" value="Rab_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043567">
        <property type="entry name" value="SYTL1-5_C2B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45716">
        <property type="entry name" value="BITESIZE, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45716:SF5">
        <property type="entry name" value="SYNAPTOTAGMIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
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        <property type="entry name" value="C2"/>
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        <property type="entry name" value="RABBD"/>
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    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
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    <feature type="domain" description="C2" evidence="8">
        <location>
            <begin position="1502"/>
            <end position="1624"/>
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    <feature type="domain" description="C2" evidence="8">
        <location>
            <begin position="1639"/>
            <end position="1768"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="7">
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            <begin position="92"/>
            <end position="349"/>
        </location>
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            <end position="392"/>
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    <feature type="region of interest" description="Disordered" evidence="7">
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            <begin position="1080"/>
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        <location>
            <begin position="1466"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
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        <source>
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    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017346601.1"/>
        </source>
    </evidence>
    <sequence length="1804" mass="202190" checksum="54BC5189AA54B80E" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SWY5</accession>
    <name>A0A2D0SWY5_ICTPU</name>
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        <submittedName>
            <fullName evidence="22">A disintegrin and metalloproteinase with thrombospondin motifs 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC108278366</name>
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    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017491703.3"/>
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        <property type="component" value="Chromosome 17"/>
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        <property type="term" value="C:extracellular matrix"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:metalloendopeptidase activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
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    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="CDD" id="cd04273">
        <property type="entry name" value="ZnMc_ADAMTS_like"/>
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        <property type="entry name" value="ADAM metallopeptidase with thrombospondin type 1 motif 9"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
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    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
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        <property type="entry name" value="ADAMTS_CR_2"/>
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        <property type="entry name" value="ADAMTS_CR_3"/>
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    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
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        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
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        <property type="entry name" value="Pept_M12B_ADAM-TS8"/>
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        <property type="entry name" value="Peptidase_M12B"/>
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    <dbReference type="InterPro" id="IPR002870">
        <property type="entry name" value="Peptidase_M12B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
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    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF41">
        <property type="entry name" value="A DISINTEGRIN AND METALLOPROTEINASE WITH THROMBOSPONDIN MOTIFS 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADAMTS_CR_2"/>
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        <property type="entry name" value="ADAMTS_CR_3"/>
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        <property type="entry name" value="ADAMTS_spacer1"/>
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        <property type="entry name" value="Pep_M12B_propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01861">
        <property type="entry name" value="ADAMTS8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="12 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 15 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 22" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 19" id="KW-0732">Signal</keyword>
    <keyword evidence="10 15 17" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039952526" evidence="19">
        <location>
            <begin position="23"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="20">
        <location>
            <begin position="218"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="192"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="active site" evidence="14 17">
        <location>
            <position position="362"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15 17">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15 17">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15 17">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="422"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="293"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="322"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="339"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="377"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="448"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="459"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="468"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="496"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="535"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="539"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="550"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017347192.2"/>
        </source>
    </evidence>
    <sequence length="938" mass="103977" checksum="2942F6F4ECC939E1" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SY26</accession>
    <name>A0A2D0SY26_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Serine/threonine-protein kinase NIM1</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">NIM1 serine/threonine-protein kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">nim1kb</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_017347589.2">
        <property type="nucleotide sequence ID" value="XM_017492100.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SY26"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006932"/>
    <dbReference type="GeneID" id="108278642"/>
    <dbReference type="KEGG" id="ipu:108278642"/>
    <dbReference type="CTD" id="797096"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14075">
        <property type="entry name" value="STKc_NIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000346">
        <property type="entry name" value="Serine/threonine-protein kinase NIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049571">
        <property type="entry name" value="NIM1K_STKc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF35">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NIM1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="69"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00080118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017347589.2"/>
        </source>
    </evidence>
    <sequence length="431" mass="48966" checksum="35F0F53C6D222AF9" modified="2023-06-28" version="2">MPARMTLVSARVPWHSLYSLTDSLDCDPDNEEEEDSSRHLTPLQKLMLDMCHDEKTIKELNVGKRVGFYKVCGEIGCGNFSKVKLGFHALTMDKVAIKILDKMRLDLQTQRMLSREISIMESLYHPNLLRLYEVLEASSRLYLVLEYAGGGDLHTRISTQGKLSDLESKLVFAQILSAVKYMHDNKIIHRDLKAENILFTSNYYVKVADFGFSTNISNCSQMLDTFCGSPPYAAPELFKDEAYMGPPVDVWAMGVLLFYMVTGTMPFRADTVPKLRRCVLQGVYVLPTWVSAPCQRLIRGILKPEPTERCALDQMLGCDWLLPVEILRSIPQLYQLNPVHLLKLGPGWAEQEEVHAALQELGVTEEHILNNQGKNSQSPLTGIYRIVLHRIQRNRGTECLPLITDVIKDPKRDGLRAYRNLRHTSKLCVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SZM4</accession>
    <name>A0A2D0SZM4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">15-hydroxyprostaglandin dehydrogenase [NAD(+)]</fullName>
            <ecNumber evidence="4">1.1.1.141</ecNumber>
            <ecNumber evidence="5">1.1.1.232</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Eicosanoid/docosanoid dehydrogenase [NAD(+)]</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Prostaglandin dehydrogenase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">zgc:56585</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">SDA103</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the NAD-dependent dehydrogenation (oxidation) of a broad array of hydroxylated polyunsaturated fatty acids (mainly eicosanoids and docosanoids, including prostaglandins, lipoxins and resolvins), yielding their corresponding keto (oxo) metabolites. Decreases the levels of the pro-proliferative prostaglandins such as prostaglandin E2 (whose activity is increased in cancer because of an increase in the expression of cyclooxygenase 2) and generates oxo-fatty acid products that can profoundly influence cell function by abrogating pro-inflammatory cytokine expression. Converts resolvins E1, D1 and D2 to their oxo products, which represents a mode of resolvin inactivation. Resolvin E1 plays important roles during the resolution phase of acute inflammation, while resolvins D1 and D2 have a unique role in obesity-induced adipose inflammation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(11R)-hydroxy-(5Z,8Z,12E,14Z)-eicosatetraenoate + NAD(+) = 11-oxo-(5Z,8Z,12E,14Z)-eicosatetraenoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48640"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78836"/>
            <dbReference type="ChEBI" id="CHEBI:90697"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:48641"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>(15S)-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + NAD(+) = 15-oxo-(5Z,8Z,11Z,13E)-eicosatetraenoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23260"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57409"/>
            <dbReference type="ChEBI" id="CHEBI:57410"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.232"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:23261"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>14-hydroxy-(4Z,7Z,10Z,12E,16Z,19Z)-docosahexaenoate + NAD(+) = 14-oxo-(4Z,7Z,10Z,12E,16Z,19Z)-docosahexaenoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48952"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:90866"/>
            <dbReference type="ChEBI" id="CHEBI:90867"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:48953"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>15-oxo-(5S,6R)-dihydroxy-(7E,9E,11Z)-eicosatrienoate + NADH + H(+) = (5S,6R,15S)-trihydroxy-(7E,9E,11Z)-eicosatrienoate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:41596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78325"/>
            <dbReference type="ChEBI" id="CHEBI:78329"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:41597"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>lipoxin A4 + NAD(+) = 15-oxo-(5S,6R)-dihydroxy-(7E,9E,11Z,13E)-eicosatetraenoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41572"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:67026"/>
            <dbReference type="ChEBI" id="CHEBI:78311"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:41573"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>prostaglandin A1 + NAD(+) = 15-oxo-prostaglandin A1 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41263"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57398"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:85072"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:41264"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>prostaglandin E1 + NAD(+) = 15-oxoprostaglandin E1 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16477"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57397"/>
            <dbReference type="ChEBI" id="CHEBI:57401"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:16478"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>prostaglandin E2 + NAD(+) = 15-oxoprostaglandin E2 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11876"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57400"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:606564"/>
            <dbReference type="EC" id="1.1.1.141"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:11877"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>resolvin D1 + NAD(+) = 17-oxoresolvin D1 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50128"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132079"/>
            <dbReference type="ChEBI" id="CHEBI:132081"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:50129"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>resolvin D1 + NAD(+) = 8-oxoresolvin D1 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50124"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132079"/>
            <dbReference type="ChEBI" id="CHEBI:132080"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:50125"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>resolvin D2 + NAD(+) = 16-oxoresolvin D2 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53588"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:133367"/>
            <dbReference type="ChEBI" id="CHEBI:137498"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:53589"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>resolvin D2 + NAD(+) = 7-oxoresolvin D2 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:133367"/>
            <dbReference type="ChEBI" id="CHEBI:137497"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:53585"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>resolvin E1 + NAD(+) = 18-oxo-resolvin E1 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49244"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:91000"/>
            <dbReference type="ChEBI" id="CHEBI:91001"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:49245"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 23">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.141" evidence="4"/>
    <dbReference type="EC" id="1.1.1.232" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017347982.1">
        <property type="nucleotide sequence ID" value="XM_017492493.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SZM4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000028191"/>
    <dbReference type="GeneID" id="108278852"/>
    <dbReference type="KEGG" id="ipu:108278852"/>
    <dbReference type="OrthoDB" id="37659at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047034">
        <property type="term" value="F:15-hydroxyicosatetraenoate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016404">
        <property type="term" value="F:15-hydroxyprostaglandin dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006693">
        <property type="term" value="P:prostaglandin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05323">
        <property type="entry name" value="ADH_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000149">
        <property type="entry name" value="15-hydroxyprostaglandin dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44229">
        <property type="entry name" value="15-HYDROXYPROSTAGLANDIN DEHYDROGENASE [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44229:SF5">
        <property type="entry name" value="15-HYDROXYPROSTAGLANDIN DEHYDROGENASE [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0644">Prostaglandin metabolism</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00049188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_017347982.1"/>
        </source>
    </evidence>
    <sequence length="265" mass="28484" checksum="13AC8FB20565AC6F" modified="2017-12-20" version="1">MLKGKVAVVSGSAQGLGKSFTHILLENGAQVAMLDVSEALGQELQVQLNQQYGSDRTQFYKADVSSDQQFTDVFQKILSKFGRIDIFCNNAGIADEENWEKTVSINLSGVVRGTYLALDHMKKQNGGNGGVIINVASLAGLGPLPSAPIYTATKYGVVGFTRAVSMASQVSGYGVRINALCPSFVRTALIDSFNQKEKTGQFHSLASLTRSLMDKSPMIEVEQVAKAFLFLVKDESMNGAAVLVRNEGAGYVKFPTDVETTPISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T1U8</accession>
    <name>A0A2D0T1U8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfate transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Solute carrier family 26 member 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21 22" type="primary">slc26a1</name>
    </gene>
    <organism evidence="20 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21 22" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21 22">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>bromide(in) + chloride(out) = bromide(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:75335"/>
            <dbReference type="ChEBI" id="CHEBI:15858"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>iodide(in) + chloride(out) = iodide(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:72379"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>nitrate(in) + chloride(out) = nitrate(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:75339"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>oxalate(in) + sulfate(out) = oxalate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:72275"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>oxalate(out) + 2 chloride(in) = oxalate(in) + 2 chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:75095"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>sulfate(out) + 2 chloride(in) = sulfate(in) + 2 chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:75091"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017348765.1">
        <property type="nucleotide sequence ID" value="XM_017493276.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017348766.1">
        <property type="nucleotide sequence ID" value="XM_017493277.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T1U8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006617"/>
    <dbReference type="GeneID" id="108279218"/>
    <dbReference type="KEGG" id="ipu:108279218"/>
    <dbReference type="CTD" id="10861"/>
    <dbReference type="OMA" id="WNENQDL"/>
    <dbReference type="OrthoDB" id="288203at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902358">
        <property type="term" value="P:sulfate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.24:FF:000015">
        <property type="entry name" value="Sulfate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00815">
        <property type="entry name" value="sulP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="183"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="427"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="478"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="19">
        <location>
            <begin position="534"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017348765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017348766.1"/>
        </source>
    </evidence>
    <sequence length="702" mass="77699" checksum="ECDD97D1C6E8939F" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T287</accession>
    <name>A0A2D0T287_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8 9">Mitotic-spindle organizing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">mzt2b</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8 9" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8 9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MOZART2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017348698.1">
        <property type="nucleotide sequence ID" value="XM_017493209.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017348699.1">
        <property type="nucleotide sequence ID" value="XM_017493210.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T287"/>
    <dbReference type="GeneID" id="108279179"/>
    <dbReference type="KEGG" id="ipu:108279179"/>
    <dbReference type="CTD" id="80097"/>
    <dbReference type="GeneTree" id="ENSGT00390000014845"/>
    <dbReference type="OrthoDB" id="10064769at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024332">
        <property type="entry name" value="MOZART2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28578:SF2">
        <property type="entry name" value="MITOTIC-SPINDLE ORGANIZING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28578">
        <property type="entry name" value="MITOTIC-SPINDLE ORGANIZING PROTEIN 2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12926">
        <property type="entry name" value="MOZART2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="98"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="104"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="146"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017348698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017348699.1"/>
        </source>
    </evidence>
    <sequence length="199" mass="21009" checksum="0E82D7BE5F8A97F6" modified="2017-12-20" version="1">MTSHNTQPGTMPTAPDSPSLSATVSGNVQKYSVKKKKVLNAEETELFELTQAAGIVMDQEVFKIIVDLLKMNVAPLAVFQTLKAMCAGQKISDTIAPDPATVTEVREEDPKVPDKSAKKATPQAPPTAPAAHPQRAGAKIVVYSPGEQSSPLSQVRSKSGTAHAEKREQRVPRQASATRGQKSAKSSGSSSSSSQLTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T6S8</accession>
    <name>A0A2D0T6S8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transmembrane protein 275</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tmem275a</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017350599.1">
        <property type="nucleotide sequence ID" value="XM_017495110.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T6S8"/>
    <dbReference type="GeneID" id="108280259"/>
    <dbReference type="KEGG" id="ipu:108280259"/>
    <dbReference type="CTD" id="101884107"/>
    <dbReference type="OMA" id="CKLFTTE"/>
    <dbReference type="OrthoDB" id="8932092at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="134"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017350599.1"/>
        </source>
    </evidence>
    <sequence length="206" mass="21542" checksum="B40FB1049029A8A1" modified="2017-12-20" version="1">MVCTEHNSGTSVPKKEPQVRNKRKSRPQGLPSPALCCACGLCIMLAGINITLVGAFAFTTLMPSGNPPIIIGPVLLLVAFTFFAACCVCSRMPPPHSSRRTKSGNAGFMRHHGATFEIETSEHTIQDTTAVQLSPTNSPSFSRSSESDRDASVNANANINANANAPAPDYSLFTMDANGPNSVSAAFSTATGSGEAVRLTLPSNAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T8I3</accession>
    <name>A0A2D0T8I3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12 13 14">Ras-related protein R-Ras isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12 13 14 15" type="primary">rras</name>
    </gene>
    <organism evidence="11 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12 13 14" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12 13 14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017351170.1">
        <property type="nucleotide sequence ID" value="XM_017495681.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017351180.1">
        <property type="nucleotide sequence ID" value="XM_017495691.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017351188.1">
        <property type="nucleotide sequence ID" value="XM_017495699.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017351197.1">
        <property type="nucleotide sequence ID" value="XM_017495708.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T8I3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009787"/>
    <dbReference type="GeneID" id="108280565"/>
    <dbReference type="KEGG" id="ipu:108280565"/>
    <dbReference type="CTD" id="6237"/>
    <dbReference type="OMA" id="GCPCILL"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04145">
        <property type="entry name" value="M_R_Ras_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000080">
        <property type="entry name" value="Ras-like GTPase Ras1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017351170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017351180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017351188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017351197.1"/>
        </source>
    </evidence>
    <sequence length="197" mass="22336" checksum="72235705A0F623F3" modified="2017-12-20" version="1">MTSEDERYKLVVVGGGGVGKSALTIQFIQSYFVSDYDPTIEDSYTKICTVDGKETRLDILDTAGQEEFGAMREQYMRSGEGFLLVFALNDVGSYNEIQKFHTQILRVKDRDDFPMVLVGNKSDLEHQRAVSREEAMSFAKENRIHYMESSAKNRYNVDEAFLEVVRAIRKFQETESPPQLANHAGKKKRGGCPCTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979ELT7</accession>
    <name>A0A979ELT7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Transient receptor potential cation channel, subfamily N, member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">trpn1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_047006463.1">
        <property type="nucleotide sequence ID" value="XM_047150507.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979ELT7"/>
    <dbReference type="GeneID" id="124626248"/>
    <dbReference type="KEGG" id="ipu:124626248"/>
    <dbReference type="CTD" id="368273"/>
    <dbReference type="OrthoDB" id="195446at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034703">
        <property type="term" value="C:cation channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070679">
        <property type="term" value="F:inositol 1,4,5 trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015279">
        <property type="term" value="F:store-operated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002153">
        <property type="entry name" value="TRPC_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10117">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10117:SF54">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL-GAMMA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01097">
        <property type="entry name" value="TRNSRECEPTRP"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5037271687" evidence="9">
        <location>
            <begin position="16"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="10">
        <location>
            <begin position="28"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_047006463.1"/>
        </source>
    </evidence>
    <sequence length="156" mass="18071" checksum="1E1E9E645B0CDDD0" modified="2023-02-22" version="1">MSVLLFFALFGLTERKDILTFHSDPIPTLTHLVFGIYLVVTVIVLLNLLIAMMSNTYQRIQSQSDTEWKFGRAILMRDITCKSETPSPFVQFTCLFYIMSLCKHRVERQDLVRKKEETEGLPEIISLCTMAHTAICRFRGRQRTQILPEGAHRECD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EMA2</accession>
    <name>A0A979EMA2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Engulfment and cell motility protein 1 isoform X4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">elmo1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_047006700.1">
        <property type="nucleotide sequence ID" value="XM_047150744.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EMA2"/>
    <dbReference type="GeneID" id="108257187"/>
    <dbReference type="CTD" id="9844"/>
    <dbReference type="OrthoDB" id="28413at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032045">
        <property type="term" value="C:guanyl-nucleotide exchange factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048870">
        <property type="term" value="P:cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032835">
        <property type="term" value="P:glomerulus development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042593">
        <property type="term" value="P:glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001946">
        <property type="term" value="P:lymphangiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045766">
        <property type="term" value="P:positive regulation of angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001212">
        <property type="term" value="P:regulation of vasculogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd13359">
        <property type="entry name" value="PH_ELMO1_CED-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000049">
        <property type="entry name" value="Engulfment and cell motility 1 (Ced-12 homolog)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000053">
        <property type="entry name" value="Engulfment and cell motility protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024574">
        <property type="entry name" value="ELMO_ARM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006816">
        <property type="entry name" value="ELMO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050868">
        <property type="entry name" value="ELMO_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12771">
        <property type="entry name" value="ENGULFMENT AND CELL MOTILITY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12771:SF23">
        <property type="entry name" value="ENGULFMENT AND CELL MOTILITY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11841">
        <property type="entry name" value="ELMO_ARM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04727">
        <property type="entry name" value="ELMO_CED12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16457">
        <property type="entry name" value="PH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51335">
        <property type="entry name" value="ELMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0581">Phagocytosis</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0729">SH3-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_047006700.1"/>
        </source>
    </evidence>
    <sequence length="740" mass="85617" checksum="ADA132A54060A81F" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EW54</accession>
    <name>A0A979EW54_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ras-related protein Rab-5B isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rab5b</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047012094.1">
        <property type="nucleotide sequence ID" value="XM_047156138.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EW54"/>
    <dbReference type="GeneID" id="108266395"/>
    <dbReference type="CTD" id="5869"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01860">
        <property type="entry name" value="Rab5_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003486">
        <property type="entry name" value="Major sperm protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="159"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_047012094.1"/>
        </source>
    </evidence>
    <sequence length="189" mass="21070" checksum="AE7F11087E6AA14D" modified="2023-02-22" version="1">MSNRGNARTNGNLPQTKICQFKLVLLGDMAVGKSSLVLRFVKGQFDEFQETTIGAAFLAQSVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVFDITKPETFERAKAWVKELQRQEAQTYAEDTGLLFMETSAKTAMNVNELFLAIAKKMPKTDTQNPTHAARHRGVNLHDPDAQSSRSCCGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EX63</accession>
    <name>A0A979EX63_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">CD99 antigen-like protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">cd99l2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Single-pass type I membrane protein</topology>
            <orientation evidence="10">Extracellular side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD99 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047012392.1">
        <property type="nucleotide sequence ID" value="XM_047156436.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EX63"/>
    <dbReference type="GeneID" id="108267554"/>
    <dbReference type="CTD" id="83692"/>
    <dbReference type="OrthoDB" id="8961553at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022078">
        <property type="entry name" value="CD99L2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15076:SF12">
        <property type="entry name" value="CD99 ANTIGEN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15076">
        <property type="entry name" value="CD99/MIC2 PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12301">
        <property type="entry name" value="CD99L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5037447343" description="CD99 antigen-like protein 2" evidence="14">
        <location>
            <begin position="24"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="38"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="151"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="68"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="183"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_047012392.1"/>
        </source>
    </evidence>
    <sequence length="287" mass="29902" checksum="CADC3138167AF34C" modified="2023-02-22" version="1">MWRFSVWSIFLVLFSLLALGGLAQELDLADALDEKLPTAKPKENEQPGGAGTGPPVMPKDPVMPKDPVMPKDPVKPKDPVKPKDPVKPPTKTTKEETLADIFTTTARPALLPRTTSRSLQTSAGPMDLDLADALVPAKDDNGFGIGDNRPGIGGGNGGGQFTDDDLANVNEDGTYHPEKGKGGRGGSGGSGGSGGVVDPADDGNYDTMAETGTIAGIVSAVAMALVGAVSSYISYQKKKLCFSIQQSLNADMVKAENPEAVVAQEPQVQQTLLQPPNAEPPTEDNVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EXB0</accession>
    <name>A0A979EXB0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein LOC108268246 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC108268246</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047012461.2">
        <property type="nucleotide sequence ID" value="XM_047156505.2"/>
    </dbReference>
    <dbReference type="GeneID" id="108268246"/>
    <dbReference type="KEGG" id="ipu:108268246"/>
    <dbReference type="OrthoDB" id="8942455at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001607">
        <property type="entry name" value="Leukocyte immune-type receptor TS32.15 L2.5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1494"/>
            <end position="1516"/>
        </location>
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        <location>
            <begin position="4"/>
            <end position="203"/>
        </location>
    </feature>
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        <location>
            <begin position="1197"/>
            <end position="1289"/>
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            <begin position="1297"/>
            <end position="1379"/>
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            <begin position="248"/>
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    <sequence length="1537" mass="178283" checksum="700FA815E8AA82A2" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EZY3</accession>
    <name>A0A979EZY3_ICTPU</name>
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        <submittedName>
            <fullName evidence="12">Uncharacterized protein LOC124626022</fullName>
        </submittedName>
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        <name evidence="12" type="primary">LOC124626022</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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            <taxon>Vertebrata</taxon>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11">SDA103</strain>
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    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <tissue evidence="12">Blood</tissue>
        </source>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="TCR_complex_component"/>
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        <property type="entry name" value="IMMUNE-TYPE RECEPTOR 5 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
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    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
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        <location>
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            <end position="143"/>
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            <begin position="6"/>
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            <begin position="177"/>
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        </location>
    </feature>
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        <location>
            <position position="1"/>
        </location>
    </feature>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_047013371.2"/>
        </source>
    </evidence>
    <sequence length="206" mass="22689" checksum="53C37E735A5303AD" modified="2023-06-28" version="2">TGDGHVKVSVFQSGVSDSVPAGASVTLQCSVLSERRSAELQVLWFRAAPPQSHPQIIYTHHNSSHQCESGSSTQTCVYNFSKNILSLNDTGTYYCAVVVCGKIIFGNGTRVQLKEPVDPVVIFLAVALGVCVIVISVQVVLFCKWRNREQHTQRQSTGQTNTQDRDSVELNYAALHFNEKKSKGGRKSRERPQDSVYSEVRSLATT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F6E1</accession>
    <name>A0A979F6E1_ICTPU</name>
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        <submittedName>
            <fullName evidence="3">Synaptotagmin-7</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">LOC124628858</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047015764.1">
        <property type="nucleotide sequence ID" value="XM_047159808.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979F6E1"/>
    <dbReference type="GeneID" id="124628858"/>
    <dbReference type="KEGG" id="ipu:124628858"/>
    <dbReference type="OrthoDB" id="270970at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_047015764.1"/>
        </source>
    </evidence>
    <sequence length="118" mass="13219" checksum="AB32014F4E31FDA5" modified="2023-02-22" version="1">MYLNREEEPNKGSVALSVFLVSLAVTVCAVWLVALCGVCGWCQRKLGKRNKPGVEAVSTPDSARGRGEKKAINDLDRDFWNNNDSNSEQQRWSSYPPKEFVLNISPYAPYGDPRLTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QWE4</accession>
    <name>A0A9F7QWE4_ICTPU</name>
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        <submittedName>
            <fullName evidence="3">CLOCK-interacting pacemaker</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">si:ch211-132b12.7</name>
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    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053534215.1">
        <property type="nucleotide sequence ID" value="XM_053678240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QWE4"/>
    <dbReference type="GeneID" id="108264013"/>
    <dbReference type="KEGG" id="ipu:108264013"/>
    <dbReference type="OrthoDB" id="6374619at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042754">
        <property type="term" value="P:negative regulation of circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031602">
        <property type="entry name" value="CIPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34648">
        <property type="entry name" value="CLOCK-INTERACTING PACEMAKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34648:SF7">
        <property type="entry name" value="SI:CH211-132B12.7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15800">
        <property type="entry name" value="CiPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="257"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053534215.1"/>
        </source>
    </evidence>
    <sequence length="488" mass="54111" checksum="ABE40F3A7EE96A33" modified="2023-06-28" version="1">MHRCSILAYCQVPTMPQELSSVGRCAAFPASRNAKDKSNNTSLLSRTKAHTQPMTNGWNSHCNSMKDSGYSEILLYSGDQDLLSFWILQELRLKGSAVTFTMDKMPPTTNLFCVPLIKTFKDMISVIISSTDAGSDYQQTDVDQHITVTELRCHKFDSMADQGNHILVSGQVEFTSMFIIKNIFLKQSRQAGQDQVLQPLWGLEGTNAQSNLLLLQQPGTVSPLHVHTLLPQRAESKNKKSKNAYLPILSSYPRIVPNLNKKKRDKPPTSREGSSEEHRLSKRVCTEETKEEISISTQVPEKLPNKQLESHFLSHSHSVLHSLSFSSDLEQRSSCLPPPELNVMGTSAFTSLSSSSVSSLKTPSLSFPSLSSCSEPAALLLPTSKVYLSGGSVPDSTSGLTRHRRFLNTVEILSQSGLLDITLRTQELLRQSAAIEQNIAQLRQHTHMLCQAVHADSNVATLEKTYRVMVESGCYPNLRSLLFMGTDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7QYT2</accession>
    <name>A0A9F7QYT2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Protein myomaker isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">mymk</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053530310.1">
        <property type="nucleotide sequence ID" value="XM_053674335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7QYT2"/>
    <dbReference type="GeneID" id="108256023"/>
    <dbReference type="KEGG" id="ipu:108256023"/>
    <dbReference type="CTD" id="389827"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021910">
        <property type="entry name" value="NGX6/PGAP6/MYMK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14319">
        <property type="entry name" value="FIVE-SPAN TRANSMEMBRANE PROTEIN M83"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14319:SF7">
        <property type="entry name" value="POST-GPI ATTACHMENT TO PROTEINS FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12036">
        <property type="entry name" value="DUF3522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039951737" evidence="8">
        <location>
            <begin position="18"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053530310.1"/>
        </source>
    </evidence>
    <sequence length="258" mass="29128" checksum="380AA55198390306" modified="2023-06-28" version="1">MKMGAFIAKMLLPTISSLVFLPAASVAAKRGFHMEAMVYFFTMFFTSIYHACDGPGLSVLCFMKYEILEYFSVYGTSISIWVTLLALGDFEEPKRSTLTMFGVLTSAVRIYQDRWGYGIYSGPIGTAVLMITVKWLQKMKEKKGLYPDKSVYTQQVGPGCCFGALALMLRFYFEEWDYAYVHSFYHLSLAVSFVLLLPKKNRYAGTGRNAAKLNCYTLCCCTSQPSVKANARSIWTTPQKPWMRACSPGLPFRHSVTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R518</accession>
    <name>A0A9F7R518_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Fucolectin-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC108260576</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a defensive agent. Recognizes blood group fucosylated oligosaccharides including A, B, H and Lewis B-type antigens. Does not recognize Lewis A antigen and has low affinity for monovalent haptens.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the fucolectin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053533542.1">
        <property type="nucleotide sequence ID" value="XM_053677567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R518"/>
    <dbReference type="GeneID" id="108260576"/>
    <dbReference type="KEGG" id="ipu:108260576"/>
    <dbReference type="OrthoDB" id="547680at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042806">
        <property type="term" value="F:fucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010185">
        <property type="term" value="P:regulation of cellular defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001868">
        <property type="term" value="P:regulation of complement activation, lectin pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051941">
        <property type="entry name" value="BG_Antigen-Binding_Lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006585">
        <property type="entry name" value="FTP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45713">
        <property type="entry name" value="FTP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45713:SF8">
        <property type="entry name" value="SI:CH211-215K15.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22633">
        <property type="entry name" value="F5_F8_type_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00607">
        <property type="entry name" value="FTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0430">Lectin</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039916697" evidence="10">
        <location>
            <begin position="25"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Fucolectin tachylectin-4 pentraxin-1" evidence="11">
        <location>
            <begin position="27"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_053533542.1"/>
        </source>
    </evidence>
    <sequence length="224" mass="25326" checksum="FE39DCBFF3ED38D9" modified="2023-06-28" version="1">MENSQRPMLLFLGICIFSLQWILTHEEVNVALGGITTQSSLYLNYYPGYFAIDGNRDSYFNSYSCSCTNSDYNPWWRVDLLAMYDISSVIITNRGDCCPKRINGAEIRIGNSLVNNGNNNPRCDVISSIPAGASANYTCKMRGRYVNIIIPNVSQFLTLCEVEVYGVPVPVTKRAFLRLKFNSSEDLMNPTVRDKVLQKMISANVQPSVLHIRWTKEPEVETET</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RGS5</accession>
    <name>A0A9F7RGS5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Histone-lysine N-methyltransferase PRDM9 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC128634969</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053541926.1">
        <property type="nucleotide sequence ID" value="XM_053685951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RGS5"/>
    <dbReference type="GeneID" id="128634969"/>
    <dbReference type="KEGG" id="ipu:128634969"/>
    <dbReference type="OrthoDB" id="9439903at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19193">
        <property type="entry name" value="PR-SET_PRDM7_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002282">
        <property type="entry name" value="Wu:fb97d07 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000512">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002005">
        <property type="entry name" value="Zinc finger protein 200"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000663">
        <property type="entry name" value="Zinc finger protein 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002604">
        <property type="entry name" value="Zinc finger protein 715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050589">
        <property type="entry name" value="Ikaros_C2H2-ZF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044417">
        <property type="entry name" value="PRDM7_9_PR-SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404:SF111">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21549">
        <property type="entry name" value="PRDM2_PR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="16">
        <location>
            <begin position="128"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="274"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="342"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="370"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="398"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="426"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="454"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="482"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="510"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="538"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="566"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_053541926.1"/>
        </source>
    </evidence>
    <sequence length="593" mass="68562" checksum="D337A622BFCAB216" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RH67</accession>
    <name>A0A9F7RH67_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform</fullName>
            <ecNumber evidence="5">2.7.1.137</ecNumber>
            <ecNumber evidence="4">2.7.1.153</ecNumber>
            <ecNumber evidence="6">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Phosphoinositide-3-kinase catalytic alpha polypeptide</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Serine/threonine protein kinase PIK3CA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="primary">zgc:158659</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30">SDA103</strain>
        </source>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="31">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>1,2-dioctanoyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ATP = 1,2-dioctanoyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55632"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83416"/>
            <dbReference type="ChEBI" id="CHEBI:83419"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:55633"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>1-octadecanoyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phospho-1D-myo-inositol 4,5-bisphosphate + ATP = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phospho-(1D-myo-inositol 3,4,5-triphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43396"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:77137"/>
            <dbReference type="ChEBI" id="CHEBI:83243"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:43397"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12709"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.137"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:12710"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.153"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:21293"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Heterodimer of a catalytic subunit PIK3CA and a p85 regulatory subunit (PIK3R1, PIK3R2 or PIK3R3). Interacts with IRS1 in nuclear extracts. Interacts with RUFY3. Interacts with RASD2. Interacts with APPL1. Interacts with HRAS and KRAS. Interaction with HRAS/KRAS is required for PI3K pathway signaling and cell proliferation stimulated by EGF and FGF2. Interacts with FAM83B; activates the PI3K/AKT signaling cascade.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.137" evidence="5"/>
    <dbReference type="EC" id="2.7.1.153" evidence="4"/>
    <dbReference type="EC" id="2.7.11.1" evidence="6"/>
    <dbReference type="RefSeq" id="XP_053542151.1">
        <property type="nucleotide sequence ID" value="XM_053686176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RH67"/>
    <dbReference type="GeneID" id="108275608"/>
    <dbReference type="KEGG" id="ipu:108275608"/>
    <dbReference type="OrthoDB" id="67688at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005943">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class IA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005944">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class IB"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016303">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046934">
        <property type="term" value="F:1-phosphatidylinositol-4,5-bisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035005">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043491">
        <property type="term" value="P:phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08398">
        <property type="entry name" value="C2_PI3K_class_I_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00872">
        <property type="entry name" value="PI3Ka_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000006">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.70:FF:000001">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000041">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000055">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000007">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000593">
        <property type="entry name" value="Zgc:158659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002420">
        <property type="entry name" value="PI3K-type_C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003113">
        <property type="entry name" value="PI3K_ABD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000341">
        <property type="entry name" value="PI3K_Ras-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF95">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4,5-BISPHOSPHATE 3-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00792">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02192">
        <property type="entry name" value="PI3K_p85B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00794">
        <property type="entry name" value="PI3K_rbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00142">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00143">
        <property type="entry name" value="PI3K_p85B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00144">
        <property type="entry name" value="PI3K_rbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51547">
        <property type="entry name" value="C2_PI3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51544">
        <property type="entry name" value="PI3K_ABD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51546">
        <property type="entry name" value="PI3K_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0581">Phagocytosis</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K-ABD" evidence="26">
        <location>
            <begin position="16"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K-RBD" evidence="28">
        <location>
            <begin position="187"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="C2 PI3K-type" evidence="29">
        <location>
            <begin position="334"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="PIK helical" evidence="27">
        <location>
            <begin position="520"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="25">
        <location>
            <begin position="768"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00065166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00073893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00076072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00078524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00083121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="RefSeq" id="XP_053542151.1"/>
        </source>
    </evidence>
    <sequence length="1072" mass="123943" checksum="E36098704E5E6B11" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RJ63</accession>
    <name>A0A9F7RJ63_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein tweety homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC108273521</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Probable chloride channel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cell membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the tweety family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053541237.1">
        <property type="nucleotide sequence ID" value="XM_053685262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RJ63"/>
    <dbReference type="GeneID" id="108273521"/>
    <dbReference type="KEGG" id="ipu:108273521"/>
    <dbReference type="OrthoDB" id="187568at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005229">
        <property type="term" value="F:intracellularly calcium-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072320">
        <property type="term" value="F:volume-sensitive chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07912">
        <property type="entry name" value="Tweety_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006990">
        <property type="entry name" value="Tweety"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12424:SF6">
        <property type="entry name" value="PROTEIN TWEETY HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12424">
        <property type="entry name" value="TWEETY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04906">
        <property type="entry name" value="Tweety"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 13" id="KW-0868">Chloride</keyword>
    <keyword evidence="9 13" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="45"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="457"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="471"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_053541237.1"/>
        </source>
    </evidence>
    <sequence length="526" mass="59026" checksum="39736B1333B322F2" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RJ83</accession>
    <name>A0A9F7RJ83_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Fc receptor-like protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC124628346</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053537806.1">
        <property type="nucleotide sequence ID" value="XM_053681831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RJ83"/>
    <dbReference type="GeneID" id="124628346"/>
    <dbReference type="KEGG" id="ipu:124628346"/>
    <dbReference type="OrthoDB" id="6151406at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:003084">
        <property type="entry name" value="leukocyte immune-type receptor 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001607">
        <property type="entry name" value="Leukocyte immune-type receptor TS32.15 L2.5a"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050488">
        <property type="entry name" value="Ig_Fc_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481:SF64">
        <property type="entry name" value="FC RECEPTOR-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481">
        <property type="entry name" value="IMMUNOGLOBULIN FC RECEPTOR"/>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <text evidence="11">Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.</text>
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<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
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        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053537702.1">
        <property type="nucleotide sequence ID" value="XM_053681727.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="128633074"/>
    <dbReference type="KEGG" id="ipu:128633074"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
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        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:transmembrane signaling receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001607">
        <property type="entry name" value="Leukocyte immune-type receptor TS32.15 L2.5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050488">
        <property type="entry name" value="Ig_Fc_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481:SF64">
        <property type="entry name" value="FC RECEPTOR-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481">
        <property type="entry name" value="IMMUNOGLOBULIN FC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="46"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="134"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053537702.1"/>
        </source>
    </evidence>
    <sequence length="260" mass="29049" checksum="A4688A28605FCCE2" modified="2023-06-28" version="1">MTVNNAGETEYKCRACSRNYESGYYGYYYYCTELSDPVRITVRARPKPVASIDPDKPVFSGDAVTLRCEVQDESWQYSWYKDDSRSSVSSEQVYTISGVEVTHTGKYTCKGAERQGSRSSHFSDAVTLTVSERPGAVLSVSPQSWLTEGDSVTLSCEITDSSTDWTFSWYTAVPYRDGLTQIKHNHGYIMYVELLSDSSRGSGGKYTLSPAALNHTGVYMCRGERGEPALHTQYSNPQPLWITGEENNSEFRLNSKGVDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RVF7</accession>
    <name>A0A9F7RVF7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein LOC108280985 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC108280985</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053544377.1">
        <property type="nucleotide sequence ID" value="XM_053688402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RVF7"/>
    <dbReference type="GeneID" id="108280985"/>
    <dbReference type="KEGG" id="ipu:108280985"/>
    <dbReference type="OrthoDB" id="8439544at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015631">
        <property type="entry name" value="CD2/SLAM_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12080">
        <property type="entry name" value="SIGNALING LYMPHOCYTIC ACTIVATION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039907180" evidence="6">
        <location>
            <begin position="20"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="418"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="147"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_053544377.1"/>
        </source>
    </evidence>
    <sequence length="459" mass="52224" checksum="84D4E16B2E0BE2BD" modified="2023-06-28" version="1">MRIQVFFGILLIFTQAAMGLLFQDPVVCRFNESSQCHVALGQRLHLQIPLEDGFELKITDKTSTDHFILKYRKTQSNPPKPNLPRWQFVKDNKTLILTSAERSDSGTYTLDTFDANGNNKGIYTLQLYVEAEVSSVKMWYSCLSPGVMKVYCSADGDNLRFNWTSDLNTFPQLENGSSTVVLNKDHHGNVTCNVENHVSRDHNTTELHPCPEPTTAVISSTTSNDGLLFQDHVVCRFNERSQCYVALGQRLHLQIPLEDVFELKITDKTSTTRSILKYRKTKRNPPKPNLPRWQFIKDDKTMILTSAESSDSGTYTLETFDANGNNKGIYTLQLNTEAKVSSVKLSYSCMFLGVRKVQCSADGENLYFSWTSEITTRLENGNSTLLLDKNCNVNITCHVENHVSHIHRSIELHSCSGFMVVFLSVWIFQVIILLSLLVGAYIYRRIYRKQRTSEIQEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TDZ0</accession>
    <name>A0A9F7TDZ0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11 12">EF-hand calcium-binding domain-containing protein 4B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 11 12" type="primary">cracr2aa</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11 12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11 12">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053533878.1">
        <property type="nucleotide sequence ID" value="XM_053677903.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053533879.1">
        <property type="nucleotide sequence ID" value="XM_053677904.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053533880.1">
        <property type="nucleotide sequence ID" value="XM_053677905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TDZ0"/>
    <dbReference type="GeneID" id="124625927"/>
    <dbReference type="CTD" id="100330395"/>
    <dbReference type="OrthoDB" id="9837699at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001129">
        <property type="entry name" value="ras-related protein Rab-44 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
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        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="75"/>
            <end position="110"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="384"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="496"/>
            <end position="537"/>
        </location>
    </feature>
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        <location>
            <begin position="195"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="399"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_053533878.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_053533879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_053533880.1"/>
        </source>
    </evidence>
    <sequence length="732" mass="83503" checksum="2519405E5B89D138" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TFK7</accession>
    <name>A0A9F7TFK7_ICTPU</name>
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        <submittedName>
            <fullName evidence="4">N-lysine methyltransferase KMT5A-A-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC128629025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053531061.1">
        <property type="nucleotide sequence ID" value="XM_053675086.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="128629025"/>
    <dbReference type="KEGG" id="ipu:128629025"/>
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        <property type="component" value="Chromosome 24"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005700">
        <property type="term" value="C:polytene chromosome"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:histone H4K20 methyltransferase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of DNA damage response, signal transduction by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051760">
        <property type="entry name" value="KMT5A"/>
    </dbReference>
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        <property type="entry name" value="SET_dom"/>
    </dbReference>
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        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46167">
        <property type="entry name" value="N-LYSINE METHYLTRANSFERASE KMT5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46167:SF1">
        <property type="entry name" value="N-LYSINE METHYLTRANSFERASE KMT5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
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    </dbReference>
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        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="2">
        <location>
            <begin position="162"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053531061.1"/>
        </source>
    </evidence>
    <sequence length="308" mass="34734" checksum="E1F07E72C861D699" modified="2023-06-28" version="1">MKQADNVVRASQLLDSLAGQTSTDPSDGESSRAARSIARSAAQQKAEMTDVQTDFEAAYSRLVEQHPVTLEGGAPDKALRADTSPSFQRKLYERWVKAQMKLRKQHIHYSEEKVSKWISDRGWRSNIPSAAQFVDEWKPHGSVDTALDAKQIRRLTRTQRWKGLIVTEDGGKGKGVIATRRFLAGEVVWDYHGQVVTASEGHSTHSTVSAEDGVHIFFFFENMNGQAMCSDAHSPCCECHPDRQTFGRLINHSRKRCNLRPRAYTLQNGVDIIIFLATKNINENEELLFDYGVERRSFIGKGLNLLWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7THF4</accession>
    <name>A0A9F7THF4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein LOC128629412</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC128629412</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SDA103</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053533342.1">
        <property type="nucleotide sequence ID" value="XM_053677367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7THF4"/>
    <dbReference type="GeneID" id="128629412"/>
    <dbReference type="KEGG" id="ipu:128629412"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_053533342.1"/>
        </source>
    </evidence>
    <sequence length="1024" mass="111303" checksum="E55DEC0319BB93BD" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TKR5</accession>
    <name>A0A9F7TKR5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein ENL</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">mllt1a</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
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                <person name="Zimin A."/>
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                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
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                <person name="Waldbieser G.C."/>
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            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Sun L."/>
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                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
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                <person name="Ke C."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
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                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="9" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[protein]-L-isoaspartate + S-adenosyl-L-methionine = [protein]-L-isoaspartate alpha-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:12705"/>
            <dbReference type="Rhea" id="RHEA-COMP:12143"/>
            <dbReference type="Rhea" id="RHEA-COMP:12144"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90596"/>
            <dbReference type="ChEBI" id="CHEBI:90598"/>
            <dbReference type="EC" id="2.1.1.77"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:12706"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.77" evidence="6"/>
    <dbReference type="EMBL" id="GU588520">
        <property type="protein sequence ID" value="ADO28476.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001188104.1">
        <property type="nucleotide sequence ID" value="NM_001201175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TDU0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000031149"/>
    <dbReference type="GeneID" id="100528905"/>
    <dbReference type="KEGG" id="ipu:100528905"/>
    <dbReference type="CTD" id="393741"/>
    <dbReference type="OMA" id="QDSPCPI"/>
    <dbReference type="OrthoDB" id="73890at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000027">
        <property type="entry name" value="Protein-L-isoaspartate O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00080">
        <property type="entry name" value="pimt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579:SF19">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01135">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01279">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2 6 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6 8" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5011323961" description="Protein-L-isoaspartate O-methyltransferase" evidence="7 10">
        <location>
            <begin position="24"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADO28476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="NP_001188104.1"/>
        </source>
    </evidence>
    <sequence length="249" mass="26749" checksum="BBCC3F8C4405ADFA" modified="2011-01-11" version="1">MWCVARLAALAFPMGLLLTRTMAWISSGKTHPQLISRLREHGVIRSDRVFEAMLATDRGIYSKDYPYEDSPQSIGYKATISAPHMHAHVLEILSEQLTEGASALDVGSGSGYLTACFARMVGPTGKVVGIDHIAELVQASVKNVQADELELLTSGRVKLVVGDGRLGFPDGAPYDAIHVGAAAPTIPKALLDQLKPGGRLVLPVGPEGGSQVLEQYDRQSDGTFVKKALMGVVYVPLTDRHHQWPGGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3TE59</accession>
    <name>E3TE59_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Exosome component 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Ribosomal RNA-processing protein 42</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">EXOS7</name>
        <name evidence="17" type="synonym">exos7</name>
        <name evidence="17" type="synonym">exosc7</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 17" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Component of the RNA exosome core complex (Exo-9), composed of EXOSC1, EXOSC2, EXOSC3, EXOSC4, EXOSC5, EXOSC6, EXOSC7, EXOSC8 and EXOSC9; within the complex interacts with EXOSC2 and EXOSC4. The catalytically inactive RNA exosome core complex (Exo-9) associates with the catalytic subunit EXOSC10/RRP6. Exo-9 may associate with DIS3 to form the nucleolar exosome complex, or DIS3L to form the cytoplasmic exosome complex. Exo-9 is formed by a hexameric base ring consisting of the heterodimers EXOSC4-EXOSC9, EXOSC5-EXOSC8 and EXOSC6-EXOSC7, and a cap ring consisting of EXOSC1, EXOSC2 and EXOSC3. The RNA exosome complex associates with cofactors C1D/RRP47, MPHOSPH6/MPP6 and MTREX/MTR4. Interacts with ZC3HAV1. Interacts with DIS3; the interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EMBL" id="GU588639">
        <property type="protein sequence ID" value="ADO28595.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001188120.1">
        <property type="nucleotide sequence ID" value="NM_001201191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TE59"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018268"/>
    <dbReference type="GeneID" id="100528921"/>
    <dbReference type="KEGG" id="ipu:100528921"/>
    <dbReference type="CTD" id="23016"/>
    <dbReference type="OrthoDB" id="272245at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034473">
        <property type="term" value="P:U1 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11367">
        <property type="entry name" value="RNase_PH_RRP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000009">
        <property type="entry name" value="Exosome complex component RRP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF8">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0271">Exosome</keyword>
    <keyword evidence="15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="13">
        <location>
            <begin position="32"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="14">
        <location>
            <begin position="196"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ADO28595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="NP_001188120.1"/>
        </source>
    </evidence>
    <sequence length="291" mass="32204" checksum="7271A7B19B287FD8" modified="2011-01-11" version="1">MATIQVSEAEKVYIMHGVRDDSRVDGRGCEDYRHMEIETDVVSNTDGSAKVSLGQTDILVGIKAEIGRPRPMVPDEGYLEFFVDCSANATPEFEGRGGEELGVELSNNLYKVFNNRHSLDLRSLCIRPGENCWVLYVDVLLLQCDGNLFDAMSIAIKAALFNTRIPNVHISEDEEGGKEIELSDDPYDCMRLNVENVPCIVTLCKIGHRHVVDATLQEKSCSVASLLISVTHKGTVTCVRKLGGGSLDPDSIFEMTETGKRVGKMLHVPLMELLQKEENLGRERTKVGFLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3TEQ2</accession>
    <name>E3TEQ2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6 8">Gamma-crystallin m2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CRGM2</name>
        <name evidence="8" type="synonym">crgm2</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="EMBL" id="GU588834">
        <property type="protein sequence ID" value="ADO28788.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187539.1">
        <property type="nucleotide sequence ID" value="NM_001200610.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TEQ2"/>
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    <dbReference type="KEGG" id="ipu:100528340"/>
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    <dbReference type="GeneTree" id="ENSGT00940000163149"/>
    <dbReference type="OrthoDB" id="8407241at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000003">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF62">
        <property type="entry name" value="CRYGM2B PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
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        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="2"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="41"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="132"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADO28788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="NP_001187539.1"/>
        </source>
    </evidence>
    <sequence length="176" mass="21819" checksum="0D7A1FECFE14B316" modified="2011-01-11" version="1">MGKVIFYEDRNFMGRSYECTSDCSDMHSYMSRCHSCRVESGCWMVYDHPNYMGNQYFMRRGEYADYMSMWGWGNNCIRSCRMIPMHRGSYRMRIYERDNFMGQMYDMSDDCDSFMDRYHWSNGCMSCHVMDGHWLMYEQPHYRGRMWYFRPGEYRNFRDYGGMRFMSMRRIMDSWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3TEU5</accession>
    <name>E3TEU5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3 5">Eukaryotic Translation initiation factor 4E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">IF4E</name>
        <name evidence="5" type="synonym">eif4e1c</name>
        <name evidence="5" type="synonym">if4e</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <dbReference type="EMBL" id="GU588877">
        <property type="protein sequence ID" value="ADO28831.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187570.1">
        <property type="nucleotide sequence ID" value="NM_001200641.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187570.1">
        <property type="nucleotide sequence ID" value="NM_001200641.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TEU5"/>
    <dbReference type="GeneID" id="100528371"/>
    <dbReference type="KEGG" id="ipu:100528371"/>
    <dbReference type="CTD" id="550549"/>
    <dbReference type="OMA" id="GNAWETE"/>
    <dbReference type="OrthoDB" id="590761at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.760.10:FF:000006">
        <property type="entry name" value="Eukaryotic translation initiation factor 4E family member 1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019770">
        <property type="entry name" value="TIF_eIF_4E_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF29">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E FAMILY MEMBER 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00813">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1 3 5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADO28831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="NP_001187570.1"/>
        </source>
    </evidence>
    <sequence length="212" mass="24371" checksum="29669C750EEA7D5C" modified="2011-01-11" version="1">MATSEPRGPESEEGSSDSPTPVAASPEQYIKHPLQNRWALWYFKNDKTKSWTENLRLISKFDTVEDFWALYNHIQQPSKLGFGCDYCLFKDGIKPMWEDDRNKLGGRWLMTLSKQQRHNDLDRYWMETLLCLIGESFDEASEDVCGAVVNVRPKGDKISIWTGNCQNKDAIMTIGQQYKERLNLPIKNILGYQSHDDTSSKSGSTTKNMYSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3TFP2</accession>
    <name>E3TFP2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pleckstrin homology domain-containing family J member 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PKHJ1</name>
        <name evidence="5" type="synonym">pkhj1</name>
        <name evidence="5" type="synonym">plekhj1</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="GU589176">
        <property type="protein sequence ID" value="ADO29128.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187793.1">
        <property type="nucleotide sequence ID" value="NM_001200864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TFP2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000028146"/>
    <dbReference type="GeneID" id="100528594"/>
    <dbReference type="KEGG" id="ipu:100528594"/>
    <dbReference type="CTD" id="55111"/>
    <dbReference type="OrthoDB" id="10055808at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001881">
        <property type="term" value="P:receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13258">
        <property type="entry name" value="PH_PLEKHJ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000300">
        <property type="entry name" value="pleckstrin homology domain-containing family J member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045188">
        <property type="entry name" value="Boi1/Boi2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22902:SF9">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY J MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22902">
        <property type="entry name" value="SESQUIPEDALIAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="15"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00041004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADO29128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="NP_001187793.1"/>
        </source>
    </evidence>
    <sequence length="153" mass="18145" checksum="7217F69FC2FC2DC3" modified="2011-01-11" version="1">MRFNEKELVFLSRQPSERAAELSMKGPKKGDVMKKRLVKLIVNFLFYFRTYEEEPIGALLLEQCRVERENDLAFSITFLDEAEREYLFECNSREQCVEWMDAIVKASYEFMRKNLIFYRSEIHRMTGKDPLEQYGISDETRFQVSNALPPPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="88" xmlns="https://uniprot.org/uniprot">
    <accession>E3TFR6</accession>
    <name>E3TFR6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Flap endonuclease 1</fullName>
            <shortName evidence="17">FEN-1</shortName>
            <ecNumber evidence="17">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Flap structure-specific endonuclease 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">FEN1B</name>
        <name evidence="23" type="synonym">fen1</name>
        <name evidence="23" type="synonym">fen1b</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 23" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14 17">Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="17">Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with PCNA. Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity. The C-terminal domain binds EP300; can bind simultaneously to both PCNA and EP300. Interacts with DDX11; this interaction is direct and increases flap endonuclease activity of FEN1. Interacts with WDR4; regulating its endonuclease activity. Interacts with POLB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Nucleus</location>
            <location evidence="17">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Nucleus</location>
            <location evidence="17">Nucleoplasm</location>
        </subcellularLocation>
        <text evidence="17">Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15 17">Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="17"/>
    <dbReference type="EMBL" id="GU589200">
        <property type="protein sequence ID" value="ADO29152.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187811.1">
        <property type="nucleotide sequence ID" value="NM_001200882.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TFR6"/>
    <dbReference type="GeneID" id="100528612"/>
    <dbReference type="KEGG" id="ipu:100528612"/>
    <dbReference type="CTD" id="2237"/>
    <dbReference type="OrthoDB" id="1937206at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09867">
        <property type="entry name" value="PIN_FEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000009">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000003">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00614">
        <property type="entry name" value="Fen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023426">
        <property type="entry name" value="Flap_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF51">
        <property type="entry name" value="FLAP ENDONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00842">
        <property type="entry name" value="XPG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7 17" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 17" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 17" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6 17 21" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9 17" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8 17 21" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 17" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 17" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="20">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="19">
        <location>
            <begin position="146"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="351"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ADO29152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="NP_001187811.1"/>
        </source>
    </evidence>
    <sequence length="380" mass="42647" checksum="7145AF2EDAC14783" modified="2011-01-11" version="1">MGIHGLAKLIADQAPTAIREQDIKNYFGRKIAIDASMCIYQFLIAVRRDGNVLQNDDGETTSHLMGMFYRTIRMLDSGIKPVYVFDGKPPQLKSGELEKRGERRAEAEKLLAQAQEMGEQENIDKFSKRLVKVTKQHNDECKKLLTLMGVPYIEAPCEAEASCAALVKAGKAYATATEDMDGLTFGTSVLLRHLTASEAKKLSIQEFHFSRVLQEMSLSHQQFIDLCILLGCDYCGTIKGIGPKRAIDLIKQYGSIEEILDNIDQSKHPAPEDWLYKEARALFLEPEVVDCTAMELKWNEPDEDGLVQFMCSEKQFSEDRIRNGCKKIMKSRRGSTQGRLDTFFTVTGSISSKRKEPEVKGSAKKKQKTSATPGKFKKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3TGF9</accession>
    <name>E3TGF9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7 9">Histidine triad nucleotide-binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">HINT1</name>
        <name evidence="9" type="synonym">hint1</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2016" name="Biol. Reprod." volume="95" first="19" last="19">
            <title>Transcriptome Display During Testicular Differentiation of Channel Catfish (Ictalurus punctatus) as Revealed by RNA-Seq Analysis.</title>
            <authorList>
                <person name="Zeng Q."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Zhang Y."/>
                <person name="Yuan Z."/>
                <person name="Jiang C."/>
                <person name="Chen A."/>
                <person name="Fu Q."/>
                <person name="Su B."/>
                <person name="Dunham R."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="27307075"/>
            <dbReference type="DOI" id="10.1095/biolreprod.116.138818"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine 5'-phosphoramidate + H2O = NH4(+) + AMP</text>
            <dbReference type="Rhea" id="RHEA:67916"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57890"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HINT family.</text>
    </comment>
    <dbReference type="EMBL" id="GU589445">
        <property type="protein sequence ID" value="ADO29395.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001188230.1">
        <property type="nucleotide sequence ID" value="NM_001201301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TGF9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000004979"/>
    <dbReference type="GeneID" id="100529031"/>
    <dbReference type="KEGG" id="ipu:100529031"/>
    <dbReference type="CTD" id="3094"/>
    <dbReference type="GeneTree" id="ENSGT00940000157905"/>
    <dbReference type="OMA" id="LAFMDVM"/>
    <dbReference type="OrthoDB" id="672793at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01276">
        <property type="entry name" value="PKCI_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000005">
        <property type="entry name" value="Histidine triad nucleotide-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23089">
        <property type="entry name" value="HISTIDINE TRIAD HIT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="6">
        <location>
            <begin position="18"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="4 5">
        <location>
            <begin position="110"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="3">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ADO29395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="NP_001188230.1"/>
        </source>
    </evidence>
    <sequence length="126" mass="13637" checksum="9BB4B7616FF2D467" modified="2011-01-11" version="1">MADEIAKAQSAQPGGDTIFGKIIRKEIPANIIYEDDQCIAFHDVAPQAPTHFLVVPRKPITQISKAEDSDAALLGHLMLVGRKCAEQVGLPNGYRMVLNEGPHGGQSVYHVHLHILGGRQLGWPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2004-07-05" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>Q6Q782</accession>
    <name>Q6Q782_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fas-associated death domain</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="6">Protein FADD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">fadd</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 6" key="1">
        <citation type="journal article" date="1999" name="Immunogenetics" volume="50" first="124" last="133">
            <title>Expanding our understanding of immunoglobulin, T-cell antigen receptor, and novel immune-type receptor genes: a subset of the immunoglobulin gene superfamily.</title>
            <authorList>
                <person name="Hawke N.A."/>
                <person name="Yoder J.A."/>
                <person name="Litman G.W."/>
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            <dbReference type="PubMed" id="10602874"/>
            <dbReference type="DOI" id="10.1007/s002510050588"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="4 6" key="2">
        <citation type="journal article" date="2004" name="Immunogenetics" volume="56" first="518" last="530">
            <title>Identification and characterization of a FasL-like protein and cDNAs encoding the channel catfish death-inducing signaling complex.</title>
            <authorList>
                <person name="Long S."/>
                <person name="Wilson M."/>
                <person name="Bengten E."/>
                <person name="Clem L.W."/>
                <person name="Miller N.W."/>
                <person name="Chinchar V.G."/>
            </authorList>
            <dbReference type="PubMed" id="15375637"/>
            <dbReference type="DOI" id="10.1007/s00251-004-0701-2"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2004-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Hawke N.A."/>
                <person name="Yoder J.A."/>
                <person name="Litman G.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="5" key="4">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="5">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="AAS84609.1"/>
        <property type="molecule type" value="mRNA"/>
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    <dbReference type="RefSeq" id="NP_001187226.1">
        <property type="nucleotide sequence ID" value="NM_001200297.1"/>
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    <dbReference type="OrthoDB" id="100767at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
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        <property type="term" value="P:apoptotic process"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Fas-associated via death domain"/>
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        <property type="entry name" value="Death Domain, Fas"/>
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        <property type="entry name" value="DEATH-like_dom_sf"/>
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        <property type="entry name" value="DED DOMAIN-CONTAINING PROTEIN"/>
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    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DED" evidence="3">
        <location>
            <begin position="3"/>
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        </location>
    </feature>
    <feature type="domain" description="Death" evidence="2">
        <location>
            <begin position="105"/>
            <end position="183"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAS84609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="NP_001187226.1"/>
        </source>
    </evidence>
    <sequence length="189" mass="22240" checksum="63E85FAD3D2E14A9" modified="2004-07-05" version="1">MDRFKFMLLEISKQLSSENLSTMKFLCGEDIGKKKREEITSGIQLFEYLIERALIGPYDTAYLRKLLSEQEALLQLIDNYEQEQPIPTDLPNETELEKINCAIEVISEQLGRKWIKYGRKLKIAETKLEGIQEKHPRNLEEQVREWFKEWMKMHKAEAKVDELIRALRDCTLNYTADVVQMNLQKANVS</sequence>
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        <name evidence="8" type="primary">FGF18</name>
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        <name type="scientific">Ictalurus punctatus</name>
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        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
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                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
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            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
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        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2">Belongs to the heparin-binding growth factors family.</text>
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        <property type="molecule type" value="mRNA"/>
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    <dbReference type="GeneID" id="108276546"/>
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    <dbReference type="OMA" id="SHRSADC"/>
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        <property type="component" value="Chromosome 16"/>
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    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="IL1HBGF"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Cytokine"/>
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    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013540085" evidence="7">
        <location>
            <begin position="28"/>
            <end position="213"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="178"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="185"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="204"/>
            <end position="213"/>
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        <source>
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        <source>
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        <source>
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            <dbReference type="SAM" id="SignalP"/>
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            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017343795.1"/>
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    </evidence>
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            <fullName evidence="6">C-type lectin domain family 4 member E</fullName>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Ictaluridae</taxon>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT408600">
        <property type="protein sequence ID" value="AHH38399.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017331629.1">
        <property type="nucleotide sequence ID" value="XM_017476140.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UA02"/>
    <dbReference type="GeneID" id="108269955"/>
    <dbReference type="KEGG" id="ipu:108269955"/>
    <dbReference type="OMA" id="CSHPLKW"/>
    <dbReference type="OrthoDB" id="6337382at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03590">
        <property type="entry name" value="CLECT_DC-SIGN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033989">
        <property type="entry name" value="CD209-like_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 6" id="KW-0430">Lectin</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="5">
        <location>
            <begin position="172"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="92"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AHH38399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017331629.1"/>
        </source>
    </evidence>
    <sequence length="309" mass="35410" checksum="43659421294D91AF" modified="2014-04-16" version="1">MVNSENQGKETYNELESQDDFTGDVQPLYSKMTRFTVSDELSSRPYRLVAIVLGVVSAAFLFAVIGLSVRINRMSDKHGILSLNTSIISSQLARLESDHKSLTESKNALQNKHDEDVKLMKSLQINLSREIRLKNELNLQKQKLEEDKRKMQSQISNLEEHCGKCLPNWVLMNSTCFYFAVSSTTPRRGWNAGRDECKKKGADLVIIDSKEKQEFIVETLKALRYNLPFSYSNGFWIGLKDDHTEGLWKWLNETTLTEGYWMDGEPNDDLTIEDCAAVYPTNNPMKAWNDAPCSHPLKWICEKEIDKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5UA04</accession>
    <name>W5UA04_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CSNK2A2</name>
        <name evidence="12" type="synonym">csnk2a2a</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="10">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JT409646">
        <property type="protein sequence ID" value="AHH38763.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017333459.1">
        <property type="nucleotide sequence ID" value="XM_017477970.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UA04"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000016707"/>
    <dbReference type="GeneID" id="108270938"/>
    <dbReference type="KEGG" id="ipu:108270938"/>
    <dbReference type="CTD" id="30488"/>
    <dbReference type="OMA" id="MDIRFYM"/>
    <dbReference type="OrthoDB" id="10254671at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905818">
        <property type="term" value="P:regulation of chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14132">
        <property type="entry name" value="STKc_CK2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000059">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000088">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045216">
        <property type="entry name" value="CK2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054:SF34">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="42"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AHH38763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017333459.1"/>
        </source>
    </evidence>
    <sequence length="351" mass="41146" checksum="03D2E7D0194A9F94" modified="2014-04-16" version="1">MPAMPGPATSKARVYADVNTLKSKDYWDYEAHVPSWSNQDDYQLVRKLGRGKYSEVFEAININSNDRVVVKILKPVKKKKIKREIKILENLRGGTNIIRLVDTVKDPVSRTPALVFECINNTDFKDLYQRLTDFDIRFYLYELLKALDYSHSMGIMHRDVKPHNVMIDHQMRKLRLIDWGLAEFYHPAQEYNVRVASRSYKGPELLVDYQMYDYSLDMWSLGCMLASMIFQKEPFFHGQDNYDQLVRIAKVLGTEELYSYLNKYHIELDTRFKDLLGQQTRKRWEHFVQPENQHLVSPEALDLLDKLLRYDHQQRLTAQEAMEHPYFYPVLKGQSLSNSDGTLAISSSTAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5UAX4</accession>
    <name>W5UAX4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">small monomeric GTPase</fullName>
            <ecNumber evidence="2">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rergl</name>
        <name evidence="9" type="synonym">rergla</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="2"/>
    <dbReference type="EMBL" id="JT410337">
        <property type="protein sequence ID" value="AHH39020.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017350403.1">
        <property type="nucleotide sequence ID" value="XM_017494914.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UAX4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000024892"/>
    <dbReference type="GeneID" id="108280140"/>
    <dbReference type="KEGG" id="ipu:108280140"/>
    <dbReference type="CTD" id="436767"/>
    <dbReference type="GeneTree" id="ENSGT00940000161146"/>
    <dbReference type="OMA" id="LDYRCQF"/>
    <dbReference type="OrthoDB" id="18798at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04146">
        <property type="entry name" value="RERG_RasL11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051065">
        <property type="entry name" value="Ras-related_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45704">
        <property type="entry name" value="RAS-LIKE FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AHH39020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017350403.1"/>
        </source>
    </evidence>
    <sequence length="205" mass="22976" checksum="BCF3A986CEE059A0" modified="2014-04-16" version="1">MNDIKVAVLGGEGVGKSALIVRFLTRRFIGEYASSSECIYRKRMSVDGRQLNLELYDPCSQPCEGKSTLNEQIHWADGFVVVYDISDRSSFITATAIVHLIRELNLGAAKRESESVVFLVGNKQDLCHMREVGKEEGQRLATDGRCQFYELSAAECYQEVALMFTKLVRNASVSGKAKERRRRPSGSKSMAKLINNVFGKRRKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5UAY4</accession>
    <name>W5UAY4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sodium-coupled neutral amino acid transporter 3</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="9 10">Sodium-coupled neutral amino acid transporter 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SLC38A3</name>
        <name evidence="9 10" type="synonym">slc38a5a</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9 10" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT410376">
        <property type="protein sequence ID" value="AHH39030.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017323613.1">
        <property type="nucleotide sequence ID" value="XM_017468124.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017323614.1">
        <property type="nucleotide sequence ID" value="XM_017468125.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UAY4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000004969"/>
    <dbReference type="GeneID" id="108265598"/>
    <dbReference type="KEGG" id="ipu:108265598"/>
    <dbReference type="CTD" id="100005797"/>
    <dbReference type="GeneTree" id="ENSGT00940000161233"/>
    <dbReference type="OMA" id="KIWKRQC"/>
    <dbReference type="OrthoDB" id="655540at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015187">
        <property type="term" value="F:glycine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015186">
        <property type="term" value="F:L-glutamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089709">
        <property type="term" value="P:L-histidine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032329">
        <property type="term" value="P:serine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF74">
        <property type="entry name" value="SODIUM-COUPLED NEUTRAL AMINO ACID TRANSPORTER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="303"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="343"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="386"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="410"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="453"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="6">
        <location>
            <begin position="51"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AHH39030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017323613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017323614.1"/>
        </source>
    </evidence>
    <sequence length="482" mass="53375" checksum="D9DFA51A1E22477B" modified="2014-04-16" version="1">MELDKMVNGHCEKRSEEDGFDCLENMAEREEFLPKKTNDKKETEFTDFVGKTSFGMSIFNLSNAIMGSGILGLAYAMSNTGIILFLILIVLIAILSAYSIHLLLKSAGVVGIRAYEQLGYRAFGSAGKILAACVITVHNIGAMSSYLFIVKMELPAVIKGLLDKEINSEWYVNGGYLIIIVSISIILPLALMRHLGYLGYTSGFSLTCMIFFLISVIYKKFVTLCSLESFSISTNTTDMPPLTTTHTPTNYTHSNTCEAKLITLTPQAAYTIPILAFAFVCHPEVLPIYTELKNPSKKRMQNVANISILAMFVMYLLTAIFGYLTFYGAVNDELLKSYSTDDTLMLCVRLAVLMAVTLTVPVVLFPIRRAVQQLLFPDKPFHWARHIAIAVVLLTLVNILVIFVPDIRDIFGVIGATSAPSLIFILPGIFYIRIVPEEQEPLKSRPKIQAAMFAALGFIFMVMSITSITIQWVAGDSSVRGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5UB75</accession>
    <name>W5UB75_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3 5">Centrosomal protein of 89 kDa</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">cep89</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT410622">
        <property type="protein sequence ID" value="AHH39135.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053532589.1">
        <property type="nucleotide sequence ID" value="XM_053676614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UB75"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035330"/>
    <dbReference type="GeneID" id="108259317"/>
    <dbReference type="KEGG" id="ipu:108259317"/>
    <dbReference type="CTD" id="84902"/>
    <dbReference type="GeneTree" id="ENSGT00940000166619"/>
    <dbReference type="OrthoDB" id="6622877at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097539">
        <property type="term" value="C:ciliary transition fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033545">
        <property type="entry name" value="CEP89"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36170">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 89 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36170:SF1">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 89 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="74"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="306"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="820"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="420"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="571"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="719"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="86"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="132"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="186"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="309"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="820"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="836"/>
            <end position="846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AHH39135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053532589.1"/>
        </source>
    </evidence>
    <sequence length="846" mass="96923" checksum="3D4B5A831300B2D1" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5UBY2</accession>
    <name>W5UBY2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12 14">Unconventional myosin-Ic</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">myo1c</name>
        <name evidence="14" type="synonym">myo1ca</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="12">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cell cortex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="EMBL" id="JT411304">
        <property type="protein sequence ID" value="AHH39375.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053532985.1">
        <property type="nucleotide sequence ID" value="XM_053677010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UBY2"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030048">
        <property type="term" value="P:actin filament-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01378">
        <property type="entry name" value="MYSc_Myo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000101">
        <property type="entry name" value="Slow myosin heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000004">
        <property type="entry name" value="Unconventional myosin ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036072">
        <property type="entry name" value="MYSc_Myo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF862">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 9" id="KW-0518">Myosin</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Myosin motor" evidence="10">
        <location>
            <begin position="29"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="domain" description="TH1" evidence="11">
        <location>
            <begin position="868"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="9">
        <location>
            <begin position="591"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="122"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AHH39375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_053532985.1"/>
        </source>
    </evidence>
    <sequence length="1048" mass="120330" checksum="66AC612F8886EDB7" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5UCR9</accession>
    <name>W5UCR9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tyrosine-protein phosphatase non-receptor type</fullName>
            <ecNumber evidence="11">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">PTPN6</name>
        <name evidence="21 22 23 24 25" type="synonym">ptpn6</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21 22 23" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21 22 23">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="11"/>
    <dbReference type="EMBL" id="JT412009">
        <property type="protein sequence ID" value="AHH39690.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017319932.1">
        <property type="nucleotide sequence ID" value="XM_017464443.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017319966.1">
        <property type="nucleotide sequence ID" value="XM_017464477.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053540525.1">
        <property type="nucleotide sequence ID" value="XM_053684550.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053540543.1">
        <property type="nucleotide sequence ID" value="XM_053684568.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053540564.1">
        <property type="nucleotide sequence ID" value="XM_053684589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UCR9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000032"/>
    <dbReference type="GeneID" id="108263538"/>
    <dbReference type="KEGG" id="ipu:108263538"/>
    <dbReference type="CTD" id="5777"/>
    <dbReference type="OrthoDB" id="8815311at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004726">
        <property type="term" value="F:non-membrane spanning protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001784">
        <property type="term" value="F:phosphotyrosine residue binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09931">
        <property type="entry name" value="SH2_C-SH2_SHP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10340">
        <property type="entry name" value="SH2_N-SH2_SHP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000012">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000018">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000045">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052123">
        <property type="entry name" value="Non-rcpt_Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012152">
        <property type="entry name" value="Tyr_Pase_non-rcpt_typ-6/11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46257">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE CORKSCREW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46257:SF4">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000929">
        <property type="entry name" value="Tyr-Ptase_nr_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7 11" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="19" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 14" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="16">
        <location>
            <begin position="4"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="16">
        <location>
            <begin position="111"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="17">
        <location>
            <begin position="248"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="18">
        <location>
            <begin position="433"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="540"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="544"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="12">
        <location>
            <position position="456"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="422"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="503"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AHH39690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017319932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017319966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_053540525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_053540543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_053540564.1"/>
        </source>
    </evidence>
    <sequence length="587" mass="67132" checksum="F6DB109FE1ED9FB6" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5UDR4</accession>
    <name>W5UDR4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">V-type proton ATPase subunit G</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ATP6V1G1</name>
        <name evidence="9" type="synonym">atp6v1g1</name>
    </gene>
    <organism evidence="7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="7">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the V-ATPase G subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="JT412854">
        <property type="protein sequence ID" value="AHH40020.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017315292.1">
        <property type="nucleotide sequence ID" value="XM_017459803.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UDR4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026613"/>
    <dbReference type="GeneID" id="108259956"/>
    <dbReference type="KEGG" id="ipu:108259956"/>
    <dbReference type="CTD" id="9550"/>
    <dbReference type="GeneTree" id="ENSGT00940000154399"/>
    <dbReference type="OMA" id="ARKYRQD"/>
    <dbReference type="OrthoDB" id="250802at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097401">
        <property type="term" value="P:synaptic vesicle lumen acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2950:FF:000001">
        <property type="entry name" value="V-type proton ATPase subunit G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005124">
        <property type="entry name" value="V-ATPase_G"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01147">
        <property type="entry name" value="V_ATP_synt_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12713:SF11">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12713">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03179">
        <property type="entry name" value="V-ATPase_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="8"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AHH40020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017315292.1"/>
        </source>
    </evidence>
    <sequence length="118" mass="13452" checksum="ABCBC3ADB242A98D" modified="2014-04-16" version="1">MASQSQGIQQLLQAEKRAAEKVAEARKRKNRRLKQAKEEAQAEIEQYRLQREKEFKTKEAAALGSHGNSAVEVDKETLDKMGRIQSSYQQNREAVLNNLLKMVCDIKPEIHANYRVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>W5UF45</accession>
    <name>W5UF45_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">STAGA complex 65 subunit gamma</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">SPTF-associated factor 65 gamma</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Suppressor of Ty 7-like</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">Supt7l</name>
        <name evidence="16 17" type="synonym">supt7l</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16 17" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16 17">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, SUPT7L, GCN5L2, TAF5L, TAF6L, TADA3L, TAD1L, TAF10, TAF12 and TAF9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT408195">
        <property type="protein sequence ID" value="AHH38267.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017332168.1">
        <property type="nucleotide sequence ID" value="XM_017476679.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017332169.1">
        <property type="nucleotide sequence ID" value="XM_017476680.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UF45"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000015255"/>
    <dbReference type="GeneID" id="108270225"/>
    <dbReference type="KEGG" id="ipu:108270225"/>
    <dbReference type="CTD" id="9913"/>
    <dbReference type="GeneTree" id="ENSGT00390000005572"/>
    <dbReference type="OMA" id="ELSWNSC"/>
    <dbReference type="OrthoDB" id="6021257at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06847">
        <property type="entry name" value="HFD_SUPT7L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000034">
        <property type="entry name" value="STAGA complex 65 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006565">
        <property type="entry name" value="BTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039460">
        <property type="entry name" value="SUPT7L/Spt7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28598">
        <property type="entry name" value="STAGA COMPLEX 65 SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28598:SF1">
        <property type="entry name" value="STAGA COMPLEX 65 SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07524">
        <property type="entry name" value="Bromo_TP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00576">
        <property type="entry name" value="BTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Bromodomain associated" evidence="13">
        <location>
            <begin position="155"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="295"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="365"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="393"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00082307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00084075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AHH38267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017332168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017332169.1"/>
        </source>
    </evidence>
    <sequence length="419" mass="46104" checksum="95958E74416130E2" modified="2014-04-16" version="1">MMRYWGELPVASVPPGRSSFDLLQREFRQVEMQDPPLHQPSARRPRPTTMLDVPSEPCSLTIHTVQLCQHTRRLRSLITAAQLGQQPPGTGSETQGILKSEDVDSLPPRPVTPAAPDDLLPLDSKAPRELFQLRHSDPESDFYKGEGEPVTELSWLSCRQLLYQSVATVLAHAGFESAMESVLETLTDLVHEHYLRLTRLLRVAVDREAHLGSTPFPDVVEQVFHEVGIGSVLALQHFWQVRIKDYHSYMLQVSKELSDEYERLVNPEKALEDSNPLKVKEEPISDLAFTLNEEAEGELTSAEQQNSMPTGVLGISDRLAGGLEDGHSPHTSGAAGNGSPLWQLQQVKMEPQDSEEGQVSGHGVLTSDVFEEGPMSTMSDAGTAPSPGGSDASCGSHSPDSLIGTSPFNQRPKKRMRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5UFM7</accession>
    <name>W5UFM7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="2 4">Nucleoredoxin-like protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">Nxnl2</name>
        <name evidence="4" type="synonym">nxnl2</name>
    </gene>
    <organism evidence="2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="2">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT414873">
        <property type="protein sequence ID" value="AHH40791.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017344842.1">
        <property type="nucleotide sequence ID" value="XM_017489353.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UFM7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008857"/>
    <dbReference type="GeneID" id="108277054"/>
    <dbReference type="KEGG" id="ipu:108277054"/>
    <dbReference type="CTD" id="158046"/>
    <dbReference type="GeneTree" id="ENSGT00940000161260"/>
    <dbReference type="OMA" id="TDDYKHE"/>
    <dbReference type="OrthoDB" id="409136at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045494">
        <property type="term" value="P:photoreceptor cell maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007608">
        <property type="term" value="P:sensory perception of smell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02964">
        <property type="entry name" value="TryX_like_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029519">
        <property type="entry name" value="RdCVF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46762">
        <property type="entry name" value="NUCLEOREDOXIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46762:SF1">
        <property type="entry name" value="NUCLEOREDOXIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13905">
        <property type="entry name" value="Thioredoxin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AHH40791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017344842.1"/>
        </source>
    </evidence>
    <sequence length="156" mass="17973" checksum="8EC80A41844EF40F" modified="2014-04-16" version="1">MAEVFSGRALLNKAGEAVDPEEALRNKVVGLYFSAGWCPPCRDFTPLLCDFYTELVEESEPPAQFEIVFISSDKSAEDMQEYYHDMHGDWLALPWTDQYKHELKKRYSITAVPKLVIVKESGQVITDKGRKQIRDQGLACFRNWLEVAEIFQNFKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>W5UHU0</accession>
    <name>W5UHU0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9 11">Lysophospholipid acyltransferase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">Mboat1</name>
        <name evidence="11" type="synonym">mboat1</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT415450">
        <property type="protein sequence ID" value="AHH41699.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017335829.1">
        <property type="nucleotide sequence ID" value="XM_017480340.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UHU0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000070"/>
    <dbReference type="GeneID" id="108272131"/>
    <dbReference type="KEGG" id="ipu:108272131"/>
    <dbReference type="CTD" id="154141"/>
    <dbReference type="OMA" id="YVVMQAA"/>
    <dbReference type="OrthoDB" id="286734at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030258">
        <property type="term" value="P:lipid modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049941">
        <property type="entry name" value="LPLAT_7/PORCN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906:SF6">
        <property type="entry name" value="LYSOPHOSPHOLIPID ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906">
        <property type="entry name" value="PORCUPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7 9 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="381"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="419"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="446"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AHH41699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017335829.1"/>
        </source>
    </evidence>
    <sequence length="487" mass="55811" checksum="684C43CC3B7C292E" modified="2014-04-16" version="1">MDPTSDLLQTTGCTWLSPISMFLGIPLDQVNFIMCQIFGLAAASWFRLCLRPQRVGPAVRHTVASFLGSLFVIFSFGWYSSHVFVLTLICYVILHRASVHTVHWYTMIVAMGYLTMCQVSRVFIFNYGILATDFSGPLMMMVQKITTLAFQLHDGKCHKQEELTAEQRRLAVITRPSLLEYLSYTLNFMSILVGPCSNYGDYTEFIEGHNVHNKLRHKLQHNGYDAQAEPSPVRAVCQKLMVCVGCLFWYFTITSRFPIGYNVDPRFISEAPFLSRLAYAFVSTQAARPKFYFAWTLADAVHNAAGYGIRGLDQNGRVSWDLVSNIHIWEIETATSFKSFIDNWNIQTGVWLKSVCYDRVPYFRTPLTFLLSALWHGVYPGYYFTFLTGIPITLTARAVRRNFRHHFLRTRAVKLAYDVITWAATQLTICYTVMPFLLLAVEPTIIYYRSMYFLVHIISILAVLVLPNRPRRPALHAQHSNNNVKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>W5UJU1</accession>
    <name>W5UJU1_ICTPU</name>
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        <submittedName>
            <fullName evidence="15 17">Nuclear receptor subfamily 1 group D member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">Nr1d2</name>
        <name evidence="17" type="synonym">nr1d2a</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR1 subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="AHH42286.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_017453731.3"/>
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    <dbReference type="OMA" id="QLECNPS"/>
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        <property type="component" value="Chromosome 23"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="NR_DBD_REV_ERB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000013">
        <property type="entry name" value="Nuclear receptor subfamily 1 group D member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050234">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001728">
        <property type="entry name" value="ThyrH_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082:SF112">
        <property type="entry name" value="NUCLEAR RECEPTOR SUBFAMILY 1 GROUP D MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00546">
        <property type="entry name" value="THYROIDHORMR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9 11 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="13">
        <location>
            <begin position="86"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="14">
        <location>
            <begin position="284"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="18"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="195"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="197"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AHH42286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017309220.1"/>
        </source>
    </evidence>
    <sequence length="521" mass="56909" checksum="D8754AC82231CE51" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5UKI8</accession>
    <name>W5UKI8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">OSBP</name>
        <name evidence="15" type="synonym">osbp</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="EMBL" id="JT417737">
        <property type="protein sequence ID" value="AHH42496.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017316496.1">
        <property type="nucleotide sequence ID" value="XM_017461007.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UKI8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026184"/>
    <dbReference type="GeneID" id="108260600"/>
    <dbReference type="CTD" id="5007"/>
    <dbReference type="OMA" id="WDEKMDY"/>
    <dbReference type="OrthoDB" id="14833at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13284">
        <property type="entry name" value="PH_OSBP_ORP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000074">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000003">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF205">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PH" evidence="12">
        <location>
            <begin position="12"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="254"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="304"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="219"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="678"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="269"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AHH42496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017316496.1"/>
        </source>
    </evidence>
    <sequence length="773" mass="86978" checksum="A33B8B4569B1D790" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>W5UKK1</accession>
    <name>W5UKK1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Spermatid perinuclear RNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">STRBP</name>
        <name evidence="16" type="synonym">strbp</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JT413298">
        <property type="protein sequence ID" value="AHH40192.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053542987.1">
        <property type="nucleotide sequence ID" value="XM_053687012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UKK1"/>
    <dbReference type="GeneID" id="108276737"/>
    <dbReference type="GeneTree" id="ENSGT00940000154687"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19909">
        <property type="entry name" value="DSRM_STRBP_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19911">
        <property type="entry name" value="DSRM_STRBP_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.40:FF:000001">
        <property type="entry name" value="interleukin enhancer-binding factor 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000006">
        <property type="entry name" value="interleukin enhancer-binding factor 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000008">
        <property type="entry name" value="interleukin enhancer-binding factor 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006561">
        <property type="entry name" value="DZF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049402">
        <property type="entry name" value="DZF_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049401">
        <property type="entry name" value="DZF_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044472">
        <property type="entry name" value="STRBP_DSRM_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45762:SF1">
        <property type="entry name" value="SPERMATID PERINUCLEAR RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45762">
        <property type="entry name" value="ZINC FINGER RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20965">
        <property type="entry name" value="DZF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07528">
        <property type="entry name" value="DZF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00572">
        <property type="entry name" value="DZF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51703">
        <property type="entry name" value="DZF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0744">Spermatogenesis</keyword>
    <feature type="domain" description="DZF" evidence="13">
        <location>
            <begin position="5"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="12">
        <location>
            <begin position="399"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="12">
        <location>
            <begin position="520"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="50"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="474"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="63"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="356"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="478"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="491"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AHH40192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_053542987.1"/>
        </source>
    </evidence>
    <sequence length="689" mass="74310" checksum="7463B571FB39F5D7" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>W5ULP4</accession>
    <name>W5ULP4_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Parvalbumin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pvalb7</name>
        <name evidence="11 12 13 14" type="synonym">pvalb6</name>
    </gene>
    <organism evidence="9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="9">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11 12 13" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11 12 13">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the parvalbumin family.</text>
    </comment>
    <dbReference type="EMBL" id="JT418816">
        <property type="protein sequence ID" value="AHH42851.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017312511.1">
        <property type="nucleotide sequence ID" value="XM_017457022.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017312517.1">
        <property type="nucleotide sequence ID" value="XM_017457028.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047005332.1">
        <property type="nucleotide sequence ID" value="XM_047149376.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053531277.1">
        <property type="nucleotide sequence ID" value="XM_053675302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ULP4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003012"/>
    <dbReference type="GeneID" id="108258409"/>
    <dbReference type="KEGG" id="ipu:108258409"/>
    <dbReference type="CTD" id="402806"/>
    <dbReference type="GeneTree" id="ENSGT00940000159653"/>
    <dbReference type="OMA" id="HRKFFQM"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000060">
        <property type="entry name" value="Parvalbumin, thymic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008080">
        <property type="entry name" value="Parvalbumin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11653">
        <property type="entry name" value="PARVALBUMIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11653:SF2">
        <property type="entry name" value="PARVALBUMIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01697">
        <property type="entry name" value="PARVALBUMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 6 7" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="39"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="78"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR608080-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AHH42851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017312511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017312517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_047005332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_053531277.1"/>
        </source>
    </evidence>
    <sequence length="109" mass="11989" checksum="FE95627A85CC355A" modified="2014-04-16" version="1">MAMSSILNADDIKKALDTFKAADSFDHKKFFEMVGLKAKSADDVKKAFHVLDADNSGFIEEEELKFVLKGFATNGRDLTDKETKAFLKAADKDGDGKIGVEEFAALVHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>W5UT90</accession>
    <name>W5UT90_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">NEDD8-activating enzyme E1 catalytic subunit</fullName>
            <ecNumber evidence="8 11">6.2.1.64</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 16" type="primary">uba3</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalytic subunit of the dimeric E1 enzyme, which activates NEDD8.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine = AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine.</text>
            <dbReference type="EC" id="6.2.1.64"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.64" evidence="8 11"/>
    <dbReference type="EMBL" id="JT418781">
        <property type="protein sequence ID" value="AHH42843.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017343317.1">
        <property type="nucleotide sequence ID" value="XM_017487828.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UT90"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000018763"/>
    <dbReference type="GeneID" id="108276284"/>
    <dbReference type="KEGG" id="ipu:108276284"/>
    <dbReference type="CTD" id="9039"/>
    <dbReference type="OrthoDB" id="5977743at2759"/>
    <dbReference type="UniPathway" id="UPA00885"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019781">
        <property type="term" value="F:NEDD8 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01488">
        <property type="entry name" value="Uba3_RUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.520:FF:000001">
        <property type="entry name" value="NEDD8-activating enzyme E1 catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000106">
        <property type="entry name" value="NEDD8-activating enzyme E1 catalytic subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.20:FF:000001">
        <property type="entry name" value="NEDD8-activating enzyme E1 catalytic subunit, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.520">
        <property type="entry name" value="Ubiquitin activating enzymes (Uba3). Chain: B, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.20">
        <property type="entry name" value="Ubiquitin-like 2 activating enzyme e1b. Chain: B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014929">
        <property type="entry name" value="E2-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023318">
        <property type="entry name" value="Ub_act_enz_dom_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030468">
        <property type="entry name" value="Uba3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033127">
        <property type="entry name" value="UBQ-activ_enz_E1_Cys_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF6">
        <property type="entry name" value="NEDD8-ACTIVATING ENZYME E1 CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08825">
        <property type="entry name" value="E2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01181">
        <property type="entry name" value="E2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00865">
        <property type="entry name" value="UBIQUITIN_ACTIVAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="E2 binding" evidence="13">
        <location>
            <begin position="371"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="10">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AHH42843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017343317.1"/>
        </source>
    </evidence>
    <sequence length="460" mass="51402" checksum="9C5319F997E35C82" modified="2014-04-16" version="1">MADGEEPAKKRQRIEEQPEKMAVDVSSSGDWDGRWSHVQKFLERSGPFTHPDFEPSEETLQFLLETCKILVIGAGGLGCELLKNLALSGFRNIHVVDMDTIDVSNLNRQFLFRTKDVGRPKAEVAADFINNRVPGCHVVPHFKKIQDFDESFYRQFHIIVCGLDSIIARRWMNGMLLSLLIYEDGVLDPSSIIPLIDGGTEGFKGNLRVIMPGMTACIDCTLELYPPQVNFPMCTIASMPRLPEHCIEYVRILQWPKEKPFGDGVALDGDNPEHIQWVFQKSLERAAEFNITGVTYRLTQGVVKRIIPAVASTNAVIAAACATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCTACSQVPQKLQFPPSAKLQEVLEYLTENASLQMKSPAITATLEGKNKTLYLQTVASIEERTRPNLCKTLKELGLADGQELAVADVTTPQTVLFKLNFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PHM8</accession>
    <name>A0A2D0PHM8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein LOC108254926 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC108254926</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017305903.1">
        <property type="nucleotide sequence ID" value="XM_017450414.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PHM8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000016979"/>
    <dbReference type="GeneID" id="108254926"/>
    <dbReference type="KEGG" id="ipu:108254926"/>
    <dbReference type="OMA" id="NWEDIDF"/>
    <dbReference type="OrthoDB" id="6157407at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042130">
        <property type="term" value="P:negative regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042102">
        <property type="term" value="P:positive regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031295">
        <property type="term" value="P:T cell costimulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051713">
        <property type="entry name" value="T-cell_Activation_Regulation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466:SF9">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466">
        <property type="entry name" value="T-LYMPHOCYTE ACTIVATION ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012429198" evidence="13">
        <location>
            <begin position="18"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="34"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="162"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="187"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017305903.1"/>
        </source>
    </evidence>
    <sequence length="220" mass="24662" checksum="59390E6217374BB3" modified="2017-12-20" version="1">MLLGFYLLLGVLHVTKGCSLTDNKKTSKVTAHVGDTVLLPCSCAAQQSPASMPTWRKINKLTKDWDSVTLNQKRFQLSNVQVPGNLSLLISHLTVEDDGLYRCQIRRDYYTDINLSVQEGSIRKPPDTMSMAFLSFIPVVLLLLGLGGVIFWRCRARKREQTPSSEGLTGQKNDEETEGKEQETQDDVTYTTVVPTDTAQRPKVVSPEERTEYAVIKLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PI14</accession>
    <name>A0A2D0PI14_ICTPU</name>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein C1orf198 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">c2h1orf198</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017305502.2">
        <property type="nucleotide sequence ID" value="XM_017450013.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PI14"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000016363"/>
    <dbReference type="GeneID" id="108254736"/>
    <dbReference type="KEGG" id="ipu:108254736"/>
    <dbReference type="CTD" id="102695047"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031600">
        <property type="entry name" value="DUF4706"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34394">
        <property type="entry name" value="SIMILAR TO RIKEN CDNA 2310022B05"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34394:SF1">
        <property type="entry name" value="SIMILAR TO RIKEN CDNA 2310022B05"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15797">
        <property type="entry name" value="DUF4706"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4706" evidence="2">
        <location>
            <begin position="22"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017305502.2"/>
        </source>
    </evidence>
    <sequence length="340" mass="37190" checksum="BE6AB18E6B1B201E" modified="2023-06-28" version="2">MAATAMNGVVADASLTEAKKNEYFSSINSMARKIMQERERIKEKYGSAWEDMSPAEQDTAIDDGMMEPKIRARYAMHRMDREELVCYPKMLIQTGQKIVHFGEEDLTWQDEHSAPFSWETKSQLEFSVITGTSEPVSSSSSSVTESKPKTSQSSKLPGIEGSGSVRRDEESAFWKLSAERSRLEGEKADFQSLTPSQIKSMEKGEKPLPSYLRSDSGSREAQEPAAPRPVKPRVSKPPAPPPPAPISVTPAPLSVNPVPLSVTPAPAAPLGGWERSQSTLPSVSTTPDDVFSPGLGTKAPSASTKDREKEDKMQPGSPTFSQYNTSSAILKTGFDFLDNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PI54</accession>
    <name>A0A2D0PI54_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Tumor necrosis factor receptor superfamily member 21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">si:dkeyp-61b2.1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017306178.1">
        <property type="nucleotide sequence ID" value="XM_017450689.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PI54"/>
    <dbReference type="GeneID" id="108255050"/>
    <dbReference type="KEGG" id="ipu:108255050"/>
    <dbReference type="OrthoDB" id="8633482at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001368">
        <property type="entry name" value="TNFR/NGFR_Cys_rich_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052459">
        <property type="entry name" value="TNFRSF_decoy_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23097:SF181">
        <property type="entry name" value="CASPASE-8-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23097">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00208">
        <property type="entry name" value="TNFR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57586">
        <property type="entry name" value="TNF receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50050">
        <property type="entry name" value="TNFR_NGFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012271445" evidence="11">
        <location>
            <begin position="25"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="8">
        <location>
            <begin position="45"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="12">
        <location>
            <begin position="45"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="185"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="46"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017306178.1"/>
        </source>
    </evidence>
    <sequence length="435" mass="47201" checksum="AD027CD4D81EF3F4" modified="2017-12-20" version="1">MKNRSSLPWLVLSVLFAAIPPAKPGPCPTCESGYGRVSGSCKCKPCTAEYFWSLRNGQGNCYKCTTSCTAKKNLIQVHPCNQTTDRRCQCKPGYYCPSPVNLTCRRDCEPCTNGFTSTSNLQTSCQQFTDCASLGKVVIKEGSPTADRVCGHPTTHKIHSIQAVNRPISQNQVSEISTVLPKLNPTSATPDTSNTTTQTLPSSILKPNSTRAVYNSVHTVYQVSQNLDRSLSFETTAPSGVSADFRMTRETKGPVPPSEPLLKTTWIFLLLLLVSLCLVTCVSLKCKSGAIKNKLEWAGLAFERYQPVLSTAHPNPPVDIVVPVSDVTDTVREVQGQGSFPGKNQKVTMEHVGKADGINNTVGSIFIYSPGMVILGSNSNEKKEEAETGTEDIGEDTCLMSVPQQESSSEEDFVRMATQEEFGKELSIPIPATSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PIE8</accession>
    <name>A0A2D0PIE8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="18">ADP-ribosylation factor-like 8Bb</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">arl8bb</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Late endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017306288.1">
        <property type="nucleotide sequence ID" value="XM_017450799.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PIE8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032386"/>
    <dbReference type="GeneID" id="108255114"/>
    <dbReference type="KEGG" id="ipu:108255114"/>
    <dbReference type="CTD" id="562445"/>
    <dbReference type="OMA" id="PRECKIP"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904115">
        <property type="term" value="C:axon cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008089">
        <property type="term" value="P:anterograde axonal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04159">
        <property type="entry name" value="Arl10_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000247">
        <property type="entry name" value="ADP-ribosylation factor-like GTPase 8A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044154">
        <property type="entry name" value="Arl8a/8b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45732:SF13">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 8B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51422">
        <property type="entry name" value="SAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="10 15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13" id="KW-0458">Lysosome</keyword>
    <keyword evidence="16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="27"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="130"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017306288.1"/>
        </source>
    </evidence>
    <sequence length="186" mass="21572" checksum="3CCC0565F0999477" modified="2017-12-20" version="1">MLALINRLLDWFRSLFWKEEMELTLVGLQYSGKTTFVNVIASGQFSEDMIPTVGFNMRKVTKGNVTIKIWDIGGQPRFRSMWERYCRGVNAIVYMVDAADRDKIEPSRNELHNLLEKPQLQGIPVLILGNKRDLPDALDEKQLIEKMNLAAIQDREICCYSVSCKEKDNIDITLQWLIHHSKSRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PK42</accession>
    <name>A0A2D0PK42_ICTPU</name>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein si:ch211-86h15.1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">si:ch211-86h15.1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017306359.2">
        <property type="nucleotide sequence ID" value="XM_017450870.3"/>
    </dbReference>
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        <property type="component" value="Chromosome 21"/>
    </dbReference>
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        <property type="entry name" value="DUF4806"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34153:SF2">
        <property type="entry name" value="SI:CH211-262H13.3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34153">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        </location>
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        </location>
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        </location>
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        <location>
            <begin position="312"/>
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        </location>
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        <location>
            <begin position="588"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="172"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017306359.2"/>
        </source>
    </evidence>
    <sequence length="611" mass="68382" checksum="C64B3251545B6100" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PNZ0</accession>
    <name>A0A2D0PNZ0_ICTPU</name>
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        <submittedName>
            <fullName evidence="12">Lysophosphatidic acid receptor 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC108255887</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="nucleotide sequence ID" value="XM_017452228.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A2D0PNZ0"/>
    <dbReference type="GeneID" id="108255887"/>
    <dbReference type="KEGG" id="ipu:108255887"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231:SF35">
        <property type="entry name" value="P2Y PURINOCEPTOR 4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231">
        <property type="entry name" value="PURINOCEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
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    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
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        <location>
            <begin position="39"/>
            <end position="294"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017307717.1"/>
        </source>
    </evidence>
    <sequence length="340" mass="39012" checksum="FA19CAEFF33C982A" modified="2017-12-20" version="1">MNCTNCYSPNNETKACPVKSQPIFISVLISLFLLLGFFLNIFSMWVFKYRTPEWKSGTVLQFHLAISDLTFCPLAPFLAVYFAMGDWPFGRDMCRLKIVLMIVHFYGNIFFLTLISIHRYISVVYHSQESRMKQKDFVKKLCIGVWAVVLIKGVVCSLLLDVSTVEKHTLCLSIHQEQYMKVYFFANFILLLPGFLIPFTISLVCYSRLARSMSGINICHQKGKLMKSKSCKMVAVCLLIFGLCFTPINVIRTVVVVVKKFYPENCRLLLHVETSYYVSWILSSANCCLDPLIYCFASQKFMGAFRSSLRKIGVRFQTIREDMENESAVSTHVVPTPRTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PPB8</accession>
    <name>A0A2D0PPB8_ICTPU</name>
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        <submittedName>
            <fullName evidence="3">Acyl-CoA dehydrogenase family member 10</fullName>
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    <gene>
        <name evidence="3" type="primary">LOC108255955</name>
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    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
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    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
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        <property type="nucleotide sequence ID" value="XM_017452358.2"/>
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    <dbReference type="STRING" id="7998.ENSIPUP00000024427"/>
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    <dbReference type="KEGG" id="ipu:108255955"/>
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    <dbReference type="CDD" id="cd05154">
        <property type="entry name" value="ACAD10_11_N-like"/>
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    </dbReference>
    <dbReference type="CDD" id="cd02603">
        <property type="entry name" value="HAD_sEH-N_like"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ACAD10-like"/>
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    <dbReference type="InterPro" id="IPR041726">
        <property type="entry name" value="ACAD10_11_N"/>
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    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
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    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
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    <dbReference type="InterPro" id="IPR011945">
        <property type="entry name" value="HAD-SF_ppase_IA/epoxid_hydro_N"/>
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    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="HAD-1A3-hyp"/>
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        <property type="entry name" value="HAD-SF-IA-v3"/>
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        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
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        <property type="entry name" value="HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12880)-RELATED"/>
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        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HADHALOGNASE"/>
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    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoglycoside phosphotransferase" evidence="1">
        <location>
            <begin position="277"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017307847.1"/>
        </source>
    </evidence>
    <sequence length="586" mass="65941" checksum="4131F2EE82DF3EA1" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PR18</accession>
    <name>A0A2D0PR18_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Golgin subfamily A member 6-like protein 24 isoform X1</fullName>
        </submittedName>
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    <gene>
        <name evidence="4" type="primary">si:ch211-102c2.8</name>
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    <organism evidence="3 4">
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        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017307876.1">
        <property type="nucleotide sequence ID" value="XM_017452387.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PR18"/>
    <dbReference type="GeneID" id="108255971"/>
    <dbReference type="KEGG" id="ipu:108255971"/>
    <dbReference type="OrthoDB" id="6158625at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159">
        <property type="entry name" value="CENTROSOMAL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159:SF31">
        <property type="entry name" value="CENTROSOME-ASSOCIATED PROTEIN CEP250 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="74"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="335"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="381"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="499"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="661"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="917"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="972"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="348"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017307876.1"/>
        </source>
    </evidence>
    <sequence length="1144" mass="130190" checksum="2C7366D9C9B210B7" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PSM3</accession>
    <name>A0A2D0PSM3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Myeloid cell surface antigen CD33 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC108256270</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017308426.1">
        <property type="nucleotide sequence ID" value="XM_017452937.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PSM3"/>
    <dbReference type="GeneID" id="108256270"/>
    <dbReference type="KEGG" id="ipu:108256270"/>
    <dbReference type="OrthoDB" id="8887244at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46013:SF4">
        <property type="entry name" value="B-CELL RECEPTOR CD22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46013">
        <property type="entry name" value="VASCULAR CELL ADHESION MOLECULE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="138"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017308426.1"/>
        </source>
    </evidence>
    <sequence length="315" mass="35035" checksum="2DC4CEB1F649B4AD" modified="2017-12-20" version="1">MNTPNTESEVLPEEEMKTLHLSVILLTLSGVLTQISGDSVKYPDNPICAVRGFSVSIPCSYFYTTSNPNIQVTQMLWCSLSTNPGWCHDPPYVYDSSSITTSDFEYAGDDKSDCTLLIHNVQFSYSGEYRFRFITNDPKIRYTGLPGAILQVSDLKVSLIRLSGIGTLKQGDSLNLTCDVNCTQSSSQFVWSKNNERLNTSGPVLHFPAVTVRDSGNYTCTWKTNMTSGSETISLLVEDDNTENPEGWPIWMIASLTVGVSVVIITLIVGAVIFNRRCVCECRSVHTEKQKKDVTNIYANVRQKEDDSDIYANVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q072</accession>
    <name>A0A2D0Q072_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tetratricopeptide repeat protein 32 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ttc32</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SDA103</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017311694.1">
        <property type="nucleotide sequence ID" value="XM_017456205.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q072"/>
    <dbReference type="GeneID" id="108257996"/>
    <dbReference type="KEGG" id="ipu:108257996"/>
    <dbReference type="CTD" id="130502"/>
    <dbReference type="OrthoDB" id="2017782at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47059">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47059:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_017311694.1"/>
        </source>
    </evidence>
    <sequence length="201" mass="22805" checksum="4C8FF4BE385C36D5" modified="2017-12-20" version="1">MEDHREVLDRQTLLNEANNEFNSHHWKRAEDLYTEFIDICMASGECDRNDLAIAYNNRGQVKYFRVDFYEAVDDYSAAIKANRQFEVAYYNRGLIRYRLASSQTAGCPKSLHTLGDCVWQHHVGRPSVSGRSWTITSSRSVRNISSLCEFVNRFGDSDVRDVVAVFPVHRDLPATLGHVGNLCTMLASKTLPVSPTLSRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q104</accession>
    <name>A0A2D0Q104_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Xylosyltransferase 2</fullName>
            <ecNumber evidence="9">2.4.2.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Xylosyltransferase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="primary">xylt2</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="29">SDA103</strain>
        </source>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="30">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="25">Catalyzes the first step in the biosynthesis of chondroitin sulfate, heparan sulfate and dermatan sulfate proteoglycans, such as DCN. Transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>UDP-alpha-D-xylose + L-seryl-[protein] = 3-O-(beta-D-xylosyl)-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50192"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:12567"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132085"/>
            <dbReference type="EC" id="2.4.2.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Glycan metabolism; chondroitin sulfate biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Glycan metabolism; heparan sulfate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the glycosyltransferase 14 family. XylT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.26" evidence="9"/>
    <dbReference type="RefSeq" id="XP_017311979.1">
        <property type="nucleotide sequence ID" value="XM_017456490.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q104"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013558"/>
    <dbReference type="GeneID" id="108258104"/>
    <dbReference type="KEGG" id="ipu:108258104"/>
    <dbReference type="CTD" id="64132"/>
    <dbReference type="OMA" id="WENAHDI"/>
    <dbReference type="OrthoDB" id="2019572at2759"/>
    <dbReference type="UniPathway" id="UPA00755"/>
    <dbReference type="UniPathway" id="UPA00756"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030158">
        <property type="term" value="F:protein xylosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050650">
        <property type="term" value="P:chondroitin sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015012">
        <property type="term" value="P:heparan sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003406">
        <property type="entry name" value="Glyco_trans_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043538">
        <property type="entry name" value="XYLT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024448">
        <property type="entry name" value="XylT_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46025:SF1">
        <property type="entry name" value="XYLOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46025">
        <property type="entry name" value="XYLOSYLTRANSFERASE OXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02485">
        <property type="entry name" value="Branch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12529">
        <property type="entry name" value="Xylo_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="22" id="KW-0464">Manganese</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0964">Secreted</keyword>
    <keyword evidence="16" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Xylosyltransferase C-terminal" evidence="28">
        <location>
            <begin position="523"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="42"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="844"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="27">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00010195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00011972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00039683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00041721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00045793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00047847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF12529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="RefSeq" id="XP_017311979.1"/>
        </source>
    </evidence>
    <sequence length="864" mass="97725" checksum="6C5E0ACA7A166689" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q200</accession>
    <name>A0A2D0Q200_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Serine/threonine-protein kinase 17A</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">DAP kinase-related apoptosis-inducing protein kinase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">stk17al</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017311711.1">
        <property type="nucleotide sequence ID" value="XM_017456222.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q200"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030565"/>
    <dbReference type="GeneID" id="108258001"/>
    <dbReference type="KEGG" id="ipu:108258001"/>
    <dbReference type="CTD" id="777621"/>
    <dbReference type="OMA" id="PFTANYE"/>
    <dbReference type="OrthoDB" id="504170at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000369">
        <property type="entry name" value="Serine/threonine kinase 17a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000175">
        <property type="entry name" value="Serine/threonine-protein kinase 17B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24342">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24342:SF16">
        <property type="entry name" value="SERINE_THREONINE KINASE 17A-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="9 16 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 21" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="30"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="295"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="297"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00076112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017311711.1"/>
        </source>
    </evidence>
    <sequence length="368" mass="41188" checksum="73CF0BEAAD431364" modified="2017-12-20" version="1">MLDSTVKNKNGMLTKIHTRIRTEPFKTNYDLVGKELGRGKFAVVKKCVEKATQNEYAAKFLRKRRKGQDCRADVLNEIAVLEGAKANPYVVGLHEVYETSSEIILVLEFAAGGEIFNQCVADNDEAFTEKDVIRLARQILTGVSFLHRNNVVHLDLKPQNILLTSAQPLGDIRIVDFGLSRHMDDKTEVREILGTPEYTAPEILSYEPISTATDMWSIGVLTYVMLTGLSPFLGEDKQETFLNVSQGNVDYSQDVFHGISDLAVDFIQSLLHLNPSERSTADECLHHAWLNTESHTHPATTTTTTTLDEQETSQSESEPETPDSSPELLDIPSYPTYPGQGDLKTGRDTFTFTESFPTRPEMQQELIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q2F5</accession>
    <name>A0A2D0Q2F5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Interferon-induced protein 44-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC108258369</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SDA103</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017312472.1">
        <property type="nucleotide sequence ID" value="XM_017456983.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q2F5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008168"/>
    <dbReference type="GeneID" id="108258369"/>
    <dbReference type="KEGG" id="ipu:108258369"/>
    <dbReference type="OrthoDB" id="25620at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00882">
        <property type="entry name" value="Ras_like_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14241">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14241:SF1">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_017312472.1"/>
        </source>
    </evidence>
    <sequence length="271" mass="30321" checksum="4A9BA97D07085F2F" modified="2017-12-20" version="1">MPWGNKDSLREKLRNFKLCSSDVKFVRLLIVGEVGAGKSSFINSVNNVFQERITSGALVDATSGTSFTKIYKTHHIKGKDGSHLPFVFNDIMGLESADGQGAHVQDIITALKGFLEEGYKFNPATPVSEKDSNYKTNPKVSDQTFCLVNVLAANKVSLMDQKLIQKMKRIREVATEYNLPQVIILTKVDETCPLVKDDLRKVYTSKKIKEKMQECSNLLGIPLSHIFPVKNYHEEVDTNDDMDVLILKALDQIVNLANDALENQNPSENSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q2R1</accession>
    <name>A0A2D0Q2R1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">E3 ubiquitin-protein ligase pellino homolog 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">peli1a</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6 7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pellino family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017312577.1">
        <property type="nucleotide sequence ID" value="XM_017457088.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053532070.1">
        <property type="nucleotide sequence ID" value="XM_053676095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q2R1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012557"/>
    <dbReference type="GeneID" id="108258444"/>
    <dbReference type="KEGG" id="ipu:108258444"/>
    <dbReference type="CTD" id="100534919"/>
    <dbReference type="GeneTree" id="ENSGT00950000183050"/>
    <dbReference type="OrthoDB" id="8801906at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008592">
        <property type="term" value="P:regulation of Toll signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006800">
        <property type="entry name" value="Pellino_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048334">
        <property type="entry name" value="Pellino_FHA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048335">
        <property type="entry name" value="Pellino_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12098:SF4">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PELLINO HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12098">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PELLINO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04710">
        <property type="entry name" value="Pellino_FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20723">
        <property type="entry name" value="Pellino_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038886">
        <property type="entry name" value="Pellino"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pellino FHA" evidence="3">
        <location>
            <begin position="5"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Pellino RING" evidence="4">
        <location>
            <begin position="269"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017312577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_053532070.1"/>
        </source>
    </evidence>
    <sequence length="432" mass="47325" checksum="F2A536D9ADDC7493" modified="2017-12-20" version="1">MLNLEEEPIKYGELIVLGCNGSFPGGNRGRRKSRFSLYRRDRANGVKPGTVHIARSPEAAKVISNNSQHSVSYALSDIETVVVEYTDDNDTDMFQIGRCTESPIDIVVANPAGCSQSNGYSGCSQSTISRFACRIVCERAPPYTARVYAAGFDCSRNIFLGEKAAKWRSFGGQMDGLTTNGVLVMRPKGEFSGSSEPGVWREISVCGNVHTLRPTRAAQSKGQQVMDESPVLVDGSLIDLCGVTLLWRAAAALSGSPGPEHLEELRMKLNAARPHCPVLLHTLAFPSLDDPESNQPRQPWVYLCCGHVHGFHPWRGRGTAIKHEEEKEENEEDVMKGAREGDKERECPLCRARGLYVPLTLGRESRLCVDAGPPSHVFVPCGHVCSEATAVYWSKTPLPCGASSFYSACPFCMKQLARDRPYVTLIFQGAPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q2Z1</accession>
    <name>A0A2D0Q2Z1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">marchf4a</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017312657.1">
        <property type="nucleotide sequence ID" value="XM_017457168.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q2Z1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032768"/>
    <dbReference type="GeneID" id="108258493"/>
    <dbReference type="KEGG" id="ipu:108258493"/>
    <dbReference type="CTD" id="100000640"/>
    <dbReference type="OMA" id="VYAHICL"/>
    <dbReference type="OrthoDB" id="264354at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046356">
        <property type="entry name" value="MARCHF4/9/11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46053">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCH4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46053:SF3">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCHF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="15">
        <location>
            <begin position="121"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="45"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017312657.1"/>
        </source>
    </evidence>
    <sequence length="394" mass="43157" checksum="0CCADA88446B3684" modified="2017-12-20" version="1">MWCAVGVGVGCCRSSLSRLPMLRREGKQRGRRCRVLFSDLKALLLRPPPPPAPSAMNGAPSSHAASPAERTRDEDDAAAAVAEWVGDAERGATESPDGGSSSEDCTKEKLDERASLESCTESGARTPLCRICFQGADQGELLSPCRCSGSVRCTHEPCLIKWISERGSWSCELCYYKYQVIAISTKNPLQWQAISLTVIEKVQIAAAVLGSLFLIASISWLVWSSLSPSAKWQRQDLLFQICYAMYGFMDLVCIALIVHEGPSVFRIFHRWQAVNQQWKVLNYDKLKDSEDPQKGSSTLRSLTLPLSNRGGVALPTSPSPSSSALTTAATVTITPGTTVPAVAMAPEDSSEVERHRAYSLLQLLSLNLRNQEPRNATTQPRNDNRELVMRVTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q4M0</accession>
    <name>A0A2D0Q4M0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">WW domain binding protein 1-like b isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">wbp1lb</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017313663.2">
        <property type="nucleotide sequence ID" value="XM_017458174.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q4M0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033766"/>
    <dbReference type="GeneID" id="108259041"/>
    <dbReference type="KEGG" id="ipu:108259041"/>
    <dbReference type="CTD" id="553658"/>
    <dbReference type="OrthoDB" id="10070083at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021684">
        <property type="entry name" value="WBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051994">
        <property type="entry name" value="WW_domain-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16209">
        <property type="entry name" value="VESICULAR, OVEREXPRESSED IN CANCER, PROSURVIVAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16209:SF4">
        <property type="entry name" value="WW DOMAIN BINDING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11669">
        <property type="entry name" value="WBP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017313663.2"/>
        </source>
    </evidence>
    <sequence length="304" mass="34703" checksum="97573BC060025CB3" modified="2023-06-28" version="2">MGLFFYTAGPVSPTEPTLDEQAQRCVGENNQSYVCESGHCCEELQCCNYYYELWWFWLMCAITFILTCCCVCHHRRAKHRLQQQQRQHEINLIAYREARNYTSPPFYFRFLPSYLLPDYEDVTNHPLTPPPPYSNLNPGPPACTNNDQEEAQCPSRQATPVPTVLTALCSSPDTVELENSRENQNRDNGKDGETMLKQDMDHKGQEQCVEVPKEKTDELVGRRRHFTGDSGIEVCLCSGRTESHRPRVLEELLRNEDDGSLMEFCDDCGARDDKDDEQGAELDASPPPPPLHPLCLSLHTINEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q8R2</accession>
    <name>A0A2D0Q8R2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribonuclease P protein subunit p29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pop4</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of nuclear RNase P and RNase MRP ribonucleoproteins. RNase P consists of a catalytic RNA moiety and 10 different protein chains; POP1, POP4, POP5, POP7, RPP14, RPP21, RPP25, RPP30, RPP38 and RPP40. Within the RNase P complex, POP1, POP7 and RPP25 form the 'finger' subcomplex, POP5, RPP14, RPP40 and homodimeric RPP30 form the 'palm' subcomplex, and RPP21, POP4 and RPP38 form the 'wrist' subcomplex. All subunits of the RNase P complex interact with the catalytic RNA. Several subunits of RNase P are also part of the RNase MRP complex. RNase MRP consists of a catalytic RNA moiety and about 8 protein subunits; POP1, POP7, RPP25, RPP30, RPP38, RPP40 and possibly also POP4 and POP5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic/archaeal RNase P protein component 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017314101.1">
        <property type="nucleotide sequence ID" value="XM_017458612.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q8R2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020120"/>
    <dbReference type="GeneID" id="108259280"/>
    <dbReference type="KEGG" id="ipu:108259280"/>
    <dbReference type="CTD" id="10775"/>
    <dbReference type="OMA" id="IPKSECV"/>
    <dbReference type="OrthoDB" id="124041at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.210:FF:000001">
        <property type="entry name" value="Ribonuclease P protein subunit p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.210">
        <property type="entry name" value="Ribonuclease P/MRP, subunit p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016848">
        <property type="entry name" value="RNase_P/MRP_Rpp29-subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036980">
        <property type="entry name" value="RNase_P/MRP_Rpp29_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023534">
        <property type="entry name" value="Rof/RNase_P-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002730">
        <property type="entry name" value="Rpp29/RNP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01868">
        <property type="entry name" value="RNase_P-MRP_p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00538">
        <property type="entry name" value="POP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101744">
        <property type="entry name" value="Rof/RNase P subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017314101.1"/>
        </source>
    </evidence>
    <sequence length="220" mass="25302" checksum="FE166C22A1995139" modified="2017-12-20" version="1">MENVVLSRLPPAESKLLGVQSQHREAAEKFTRAFLNSSVPRLRCKEAEDMLKHKAVMLQFARERKRRGTKTKAKGLNAKEKRALRVFQLKPEHQKYDLFLPLHDLWKQYITDLCNGLRPDSNPQVMQQKLLKADFHGAVLTVVRSKCPSYVGSTGILVQELKHVFKIITKENRLKIIPKRNSVFSVEMGDFVAHIYGSKFELRSSERSAKKFKTKGTIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q9E3</accession>
    <name>A0A2D0Q9E3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Alpha-crystallin B chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Alpha(B)-crystallin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">cryabb</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7 9 10">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017314914.1">
        <property type="nucleotide sequence ID" value="XM_017459425.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q9E3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033417"/>
    <dbReference type="GeneID" id="108259721"/>
    <dbReference type="KEGG" id="ipu:108259721"/>
    <dbReference type="CTD" id="436943"/>
    <dbReference type="OMA" id="FRDWWED"/>
    <dbReference type="OrthoDB" id="1431247at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060047">
        <property type="term" value="P:heart contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007626">
        <property type="term" value="P:locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036438">
        <property type="term" value="P:maintenance of lens transparency"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030239">
        <property type="term" value="P:myofibril assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007519">
        <property type="term" value="P:skeletal muscle tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd06478">
        <property type="entry name" value="ACD_HspB4-5-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055269">
        <property type="entry name" value="Alpha-crystallin/HSP_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001436">
        <property type="entry name" value="Alpha-crystallin/sHSP_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003090">
        <property type="entry name" value="Alpha-crystallin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640:SF5">
        <property type="entry name" value="ALPHA-CRYSTALLIN B CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45640">
        <property type="entry name" value="HEAT SHOCK PROTEIN HSP-12.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00525">
        <property type="entry name" value="Crystallin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036514">
        <property type="entry name" value="Sm_HSP_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00299">
        <property type="entry name" value="ACRYSTALLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SHSP" evidence="11">
        <location>
            <begin position="53"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR036514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR036514-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017314914.1"/>
        </source>
    </evidence>
    <sequence length="172" mass="19846" checksum="176E426D2E0BD90B" modified="2017-12-20" version="1">MDIAIQHPWFRRSFWTSFFPTRIFDQHFGEHISESDVLAPYPSLYFPRPSFFRWPSWVDSGLSEMRLEKDCFTINLDIKHFAPEELSVKISGDYIQVHAKHEDRQDDHGFVSREFLRKYRVPSGVDPASITSSISSDGVLTITAPRKASDAPERFISITREDKPAVSGPQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QA25</accession>
    <name>A0A2D0QA25_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5 6">Zinc finger SWIM domain-containing protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5 6" type="primary">zswim7</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4 5 6" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4 5 6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017315152.1">
        <property type="nucleotide sequence ID" value="XM_017459663.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017315153.1">
        <property type="nucleotide sequence ID" value="XM_017459664.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017315154.1">
        <property type="nucleotide sequence ID" value="XM_017459665.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QA25"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035973"/>
    <dbReference type="GeneID" id="108259887"/>
    <dbReference type="KEGG" id="ipu:108259887"/>
    <dbReference type="CTD" id="125150"/>
    <dbReference type="OMA" id="YTCYTSC"/>
    <dbReference type="OrthoDB" id="337581at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097196">
        <property type="term" value="C:Shu complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28498">
        <property type="entry name" value="ZINC FINGER SWIM DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28498:SF1">
        <property type="entry name" value="ZINC FINGER SWIM DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="76"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017315152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017315153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017315154.1"/>
        </source>
    </evidence>
    <sequence length="140" mass="15337" checksum="67FC864AFCEE4D80" modified="2017-12-20" version="1">MASCLPAVAEQLLQDLQKTYSEKSQIPDELLIALRFIFGSCTLQALDLVDQRAVTCVSSPSGRVVFQVLGGSGRLYTCYTSCHYCPCPAFAFSVLRRNESLVCKHILAAYLCQAMGLCQQEQVSDQHMTLILSGRAALDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QCC3</accession>
    <name>A0A2D0QCC3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIPF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">yipf6</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017315040.1">
        <property type="nucleotide sequence ID" value="XM_017459551.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QCC3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000024548"/>
    <dbReference type="GeneID" id="108259836"/>
    <dbReference type="KEGG" id="ipu:108259836"/>
    <dbReference type="CTD" id="286451"/>
    <dbReference type="OMA" id="IKFYHVL"/>
    <dbReference type="OrthoDB" id="411251at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045231">
        <property type="entry name" value="Yip1/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236">
        <property type="entry name" value="GOLGI MEMBRANE PROTEIN YIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236:SF1">
        <property type="entry name" value="PROTEIN YIPF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="7">
        <location>
            <begin position="69"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017315040.1"/>
        </source>
    </evidence>
    <sequence length="229" mass="24837" checksum="A8704581B42D0064" modified="2017-12-20" version="1">MEDVDEINKPLFAGLSDVSIADDIPVEGDITVPMSSPSVNDEISTLDEPVKDTILRDLKAVGQKFMHVMYPKKSSALLRDWDLWGPLLLCVALALMLQGGAADSKDDGGPQFAEVFVIIWFGSIVITINSKLLGGTISFFQSLCVLGYCILPLTAAMLVCRLVLLGSNGLVSFIVRLVVVTASFGWSTFASTAFLADSQPPNRKALVVYPVFLFYFVIGWMILTFSASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QCC9</accession>
    <name>A0A2D0QCC9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">acetyl-CoA carboxylase</fullName>
            <ecNumber evidence="4">6.4.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">acaca</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.4.1.2" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017315365.1">
        <property type="nucleotide sequence ID" value="XM_017459876.3"/>
    </dbReference>
    <dbReference type="GeneID" id="108259993"/>
    <dbReference type="KEGG" id="ipu:108259993"/>
    <dbReference type="CTD" id="31"/>
    <dbReference type="OrthoDB" id="14612at2759"/>
    <dbReference type="UniPathway" id="UPA00655">
        <property type="reaction ID" value="UER00711"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001295">
        <property type="term" value="P:malonyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.460.10:FF:000001">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000005">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000005">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1770.10:FF:000001">
        <property type="entry name" value="acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000003">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000005">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000010">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.460.10">
        <property type="entry name" value="Biotin dependent carboxylase carboxyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1770.10">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049076">
        <property type="entry name" value="ACCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049074">
        <property type="entry name" value="ACCA_BT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013537">
        <property type="entry name" value="AcCoA_COase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728:SF5">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08326">
        <property type="entry name" value="ACC_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21385">
        <property type="entry name" value="ACCA_BT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0092">Biotin</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="8" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="46"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017315365.1"/>
        </source>
    </evidence>
    <sequence length="2395" mass="269508" checksum="B47676F6AFDD23AC" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QEF7</accession>
    <name>A0A2D0QEF7_ICTPU</name>
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        <submittedName>
            <fullName evidence="9 10 11">Sister chromatid cohesion protein PDS5 homolog A isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10 11 12" type="primary">pds5a</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
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    <reference evidence="9 10 11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10 11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017461185.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017316675.1">
        <property type="nucleotide sequence ID" value="XM_017461186.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017316676.1">
        <property type="nucleotide sequence ID" value="XM_017461187.3"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_047152427.2"/>
    </dbReference>
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    <dbReference type="KEGG" id="ipu:108260719"/>
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        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19953">
        <property type="entry name" value="PDS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001146">
        <property type="entry name" value="PDS5 cohesin associated factor B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000064">
        <property type="entry name" value="Sister chromatid cohesion protein PDS5 homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ARM-like"/>
    </dbReference>
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        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039776">
        <property type="entry name" value="Pds5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12663">
        <property type="entry name" value="ANDROGEN INDUCED INHIBITOR OF PROLIFERATION AS3 / PDS5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12663:SF2">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN PDS5 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1156"/>
            <end position="1445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1156"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1190"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1229"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
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        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017316674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017316675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017316676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_047008383.1"/>
        </source>
    </evidence>
    <sequence length="1445" mass="163012" checksum="3F987417D1F5290E" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QPL9</accession>
    <name>A0A2D0QPL9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">N-acylneuraminate-9-phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nanp</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="RefSeq" id="XP_017320259.1">
        <property type="nucleotide sequence ID" value="XM_017464770.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QPL9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026880"/>
    <dbReference type="GeneID" id="108263708"/>
    <dbReference type="KEGG" id="ipu:108263708"/>
    <dbReference type="CTD" id="140838"/>
    <dbReference type="OMA" id="PQETHDT"/>
    <dbReference type="OrthoDB" id="1694274at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050124">
        <property type="term" value="F:N-acylneuraminate-9-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046380">
        <property type="term" value="P:N-acetylneuraminate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04305">
        <property type="entry name" value="HAD_Neu5Ac-Pase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.710">
        <property type="entry name" value="Haloacid dehalogenase hydrolase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051400">
        <property type="entry name" value="HAD-like_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011950">
        <property type="entry name" value="HAD-SF_hydro_IA_CTE7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02253">
        <property type="entry name" value="CTE7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46470">
        <property type="entry name" value="N-ACYLNEURAMINATE-9-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46470:SF3">
        <property type="entry name" value="N-ACYLNEURAMINATE-9-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017320259.1"/>
        </source>
    </evidence>
    <sequence length="241" mass="26696" checksum="3F846C074AC2A6C9" modified="2017-12-20" version="1">MEKGGISAILFDLDNTLVDTAGAGQLALHKVCELLRSKLKDTNSSDICKRFTRKLYEEKFDPSAGITIDDVRINHWHEALQEAGSVDPGRALASDCYNTWKTTRLQALTLSPEVLALLRYLKCSYKLLLLTNGDAQTQREKLEAVKCEGLFSAVVVGGEHAEQKPAVSIFTHCFELLGVKPQDCIMVGDSLDTDIQGGFDARVRATVWINKSIDTRPENSVTPDFTIRSVLDLRNILAELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QQW0</accession>
    <name>A0A2D0QQW0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein LOC108263503 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC108263503</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017319855.2">
        <property type="nucleotide sequence ID" value="XM_017464366.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QQW0"/>
    <dbReference type="GeneID" id="108263503"/>
    <dbReference type="KEGG" id="ipu:108263503"/>
    <dbReference type="OrthoDB" id="8446208at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054554">
        <property type="entry name" value="ZP1/4_Ig-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22821">
        <property type="entry name" value="ZP1_ZP4_Ig-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Zona pellucida sperm-binding protein 1/4 Ig-like" evidence="6">
        <location>
            <begin position="126"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="363"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF22821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017319855.2"/>
        </source>
    </evidence>
    <sequence length="574" mass="60969" checksum="ABE4CED102E95586" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QV28</accession>
    <name>A0A2D0QV28_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fibroblast growth factor</fullName>
            <shortName evidence="4">FGF</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">fgf7</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6 7">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017321591.1">
        <property type="nucleotide sequence ID" value="XM_017466102.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017321592.1">
        <property type="nucleotide sequence ID" value="XM_017466103.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QV28"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000024091"/>
    <dbReference type="GeneID" id="108264500"/>
    <dbReference type="KEGG" id="ipu:108264500"/>
    <dbReference type="CTD" id="2252"/>
    <dbReference type="GeneTree" id="ENSGT00940000159843"/>
    <dbReference type="OMA" id="CSKHERY"/>
    <dbReference type="OrthoDB" id="5987799at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23306">
        <property type="entry name" value="beta-trefoil_FGF7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00263">
        <property type="entry name" value="HBGFFGF"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00442">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013419718" description="Fibroblast growth factor" evidence="4">
        <location>
            <begin position="34"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU049442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017321591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017321592.1"/>
        </source>
    </evidence>
    <sequence length="203" mass="23160" checksum="DC565462391EAFB8" modified="2017-12-20" version="1">MHRWMVAWKRPDLLCGLYLRVLLWLGGVGVSHGGGVASTEPLAVCDCIKQERHARNYDYMEGGDVRIRRLYSRTQWFLTVDENGNVNGTQDPNNCYSILEIRTVSEGGVLAIKGVKSQYYISMSRSGILQGKKEYSDSCNFKEVFLENFYTAYMSEKWSKPGKEVFVSLSQKGRPLRGKKTRKENIASHFIPRTCKEDDRTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QWW6</accession>
    <name>A0A2D0QWW6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Centrosomal protein of 104 kDa</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">cep104</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Required for ciliogenesis and for structural integrity at the ciliary tip.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with CCP110 and CEP97. Interacts with ARMC9, TOGARAM1, CCDC66 and CSPP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017322902.1">
        <property type="nucleotide sequence ID" value="XM_017467413.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QWW6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000010830"/>
    <dbReference type="GeneID" id="108265271"/>
    <dbReference type="KEGG" id="ipu:108265271"/>
    <dbReference type="CTD" id="9731"/>
    <dbReference type="OMA" id="VQGNDYN"/>
    <dbReference type="OrthoDB" id="66599at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000200">
        <property type="entry name" value="Centrosomal protein of 104 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052607">
        <property type="entry name" value="CEP104-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048739">
        <property type="entry name" value="CEP104_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048738">
        <property type="entry name" value="CEP104_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13371:SF0">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 104 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13371">
        <property type="entry name" value="GLYCINE-, GLUTAMATE-, THIENYLCYCLOHEXYLPIPERIDINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21040">
        <property type="entry name" value="CEP104-like_TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21038">
        <property type="entry name" value="CEP104_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21039">
        <property type="entry name" value="CEP104_ZnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TOG" evidence="13">
        <location>
            <begin position="423"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="350"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="403"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="711"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="904"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="711"/>
            <end position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00065345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017322902.1"/>
        </source>
    </evidence>
    <sequence length="941" mass="106221" checksum="E67B828785437955" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R0F1</accession>
    <name>A0A2D0R0F1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA (cytosine-5-)-methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26 27" type="primary">dnmt3ba</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">SDA103</strain>
        </source>
    </reference>
    <reference evidence="26 27" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26 27">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MAPRE family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.37" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017324022.1">
        <property type="nucleotide sequence ID" value="XM_017468533.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053536293.1">
        <property type="nucleotide sequence ID" value="XM_053680318.1"/>
    </dbReference>
    <dbReference type="GeneID" id="108265819"/>
    <dbReference type="KEGG" id="ipu:108265819"/>
    <dbReference type="CTD" id="321084"/>
    <dbReference type="GeneTree" id="ENSGT00940000166425"/>
    <dbReference type="OrthoDB" id="641149at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051718">
        <property type="term" value="F:DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061371">
        <property type="term" value="P:determination of heart left/right asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000008">
        <property type="entry name" value="DNA (Cytosine-5)-methyltransferase 3A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000011">
        <property type="entry name" value="DNA methyltransferase 3 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000028">
        <property type="entry name" value="RP/EB family microtubule-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.50">
        <property type="entry name" value="PWWP, helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025766">
        <property type="entry name" value="ADD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050390">
        <property type="entry name" value="C5-Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018117">
        <property type="entry name" value="C5_DNA_meth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040552">
        <property type="entry name" value="DNMT3_ADD_GATA1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049554">
        <property type="entry name" value="DNMT3_ADD_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23068:SF53">
        <property type="entry name" value="DNA (CYTOSINE-5-)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23068">
        <property type="entry name" value="DNA CYTOSINE-5- -METHYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17980">
        <property type="entry name" value="ADD_DNMT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21255">
        <property type="entry name" value="DNMT3_ADD_GATA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51533">
        <property type="entry name" value="ADD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00094">
        <property type="entry name" value="C5_MTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 20" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="14" id="KW-0498">Mitosis</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="10 20" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="9 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <keyword evidence="13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="22">
        <location>
            <begin position="34"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="PWWP" evidence="23">
        <location>
            <begin position="940"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="24">
        <location>
            <begin position="1109"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="158"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="782"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="903"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1060"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="160"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1063"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="21">
        <location>
            <begin position="1085"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="active site" evidence="20">
        <location>
            <position position="1337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01016"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
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    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
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    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51533"/>
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    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_017324022.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_053536293.1"/>
        </source>
    </evidence>
    <sequence length="1540" mass="174022" checksum="9E64D04E8E14AA6A" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R384</accession>
    <name>A0A2D0R384_ICTPU</name>
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        <submittedName>
            <fullName evidence="25">Neuronal acetylcholine receptor subunit beta-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">chrnb2</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">SDA103</strain>
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    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="25">Blood</tissue>
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    </reference>
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        <reaction evidence="19">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="1">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily.</text>
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        <property type="nucleotide sequence ID" value="XM_017468776.3"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0004888">
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0007271">
        <property type="term" value="P:synaptic transmission, cholinergic"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="LGIC_ECD_nAChR_B2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="LGIC_TM_nAChR"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cholinergic receptor nicotinic beta 2 subunit"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cholinergic receptor nicotinic beta 4 subunit"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Nicotinic acetylcholine receptor subunit alpha4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Neur_chan_lig-bd"/>
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        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
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    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
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    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
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    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
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        <property type="entry name" value="NRIONCHANNEL"/>
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    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15 20" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 20" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="11 25" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 20" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 20" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022256605" evidence="20">
        <location>
            <begin position="20"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="480"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="22">
        <location>
            <begin position="23"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="23">
        <location>
            <begin position="235"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="399"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
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    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
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    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
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    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="RefSeq" id="XP_017324265.1"/>
        </source>
    </evidence>
    <sequence length="514" mass="57870" checksum="FA7ED473329042B3" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R5H9</accession>
    <name>A0A2D0R5H9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein si:dkey-192g7.3 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">si:dkey-192g7.3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="16">Blood</tissue>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:cellular response to lipopolysaccharide"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="BUTYROPHILIN SUBFAMILY 2 MEMBER A2-LIKE-RELATED"/>
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        <location>
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        </source>
    </evidence>
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    <sequence length="203" mass="23237" checksum="A8D3240EE8C14546" modified="2017-12-20" version="1">MTRSAIVVWLLAVSYMNSRAAEQIQNMSVFTGENVILPCPCSEDTKNLVWQIGEEIVVNHCCKEKDPLHESYVNRTQVFLSFSKGNCSLLLRDVSNNDQRIFTCYVLDQEKRLKHMHKVRLIVEVAKESNRSTINDLSTTTESHDEPHRGFSVGVLFSLVMILVILAGLIVVLLIRKHQQRRPRMEVKQDPQAKLPIMDHTPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R6B8</accession>
    <name>A0A2D0R6B8_ICTPU</name>
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        <name evidence="9" type="primary">gpr155a</name>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="9">Blood</tissue>
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    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="component" value="Chromosome 6"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="DEP_dom"/>
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        <property type="entry name" value="Mem_transp_PIN-like"/>
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        <property type="entry name" value="mTOR-Rac_regulators"/>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
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        <property type="entry name" value="DEP DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="82"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        </location>
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        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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    <feature type="transmembrane region" description="Helical" evidence="6">
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        </location>
    </feature>
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        <location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="675"/>
            <end position="696"/>
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    </feature>
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        <location>
            <begin position="758"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017325355.1"/>
        </source>
    </evidence>
    <sequence length="824" mass="91130" checksum="A6F26B71DA62A531" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RHZ6</accession>
    <name>A0A2D0RHZ6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">CCN family member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC108269094</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017330149.1">
        <property type="nucleotide sequence ID" value="XM_017474660.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RHZ6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000050"/>
    <dbReference type="GeneID" id="108269094"/>
    <dbReference type="KEGG" id="ipu:108269094"/>
    <dbReference type="OMA" id="PRCNHDL"/>
    <dbReference type="OrthoDB" id="365605at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002062">
        <property type="term" value="P:chondrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045597">
        <property type="term" value="P:positive regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CCN"/>
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        <property type="entry name" value="Cys_knot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006208">
        <property type="entry name" value="Glyco_hormone_CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043973">
        <property type="entry name" value="TSP1_CCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11348:SF8">
        <property type="entry name" value="CCN FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11348">
        <property type="entry name" value="CONNECTIVE TISSUE GROWTH FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00007">
        <property type="entry name" value="Cys_knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19035">
        <property type="entry name" value="TSP1_CCN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00093">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00041">
        <property type="entry name" value="CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01185">
        <property type="entry name" value="CTCK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01225">
        <property type="entry name" value="CTCK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012293862" evidence="8">
        <location>
            <begin position="33"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="10">
        <location>
            <begin position="67"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="CTCK" evidence="9">
        <location>
            <begin position="221"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017330149.1"/>
        </source>
    </evidence>
    <sequence length="315" mass="34856" checksum="8CB55BD312079916" modified="2017-12-20" version="1">MFSNSFMKKEVPLSVCAQVLLFCAVMCVRQRGEPCSQTKPCQKGLRCRYMGGNGNRTRTVCAAPDADSCVLGNSVYHSGETFFPSCRYQCVCHGGQISCVPRCNLDVMLPGPDCPFPRKVQVLGECCEKWVCDAQAEVSVLGAFAMAAYRQEETLGFDPSMECMEQSTEWSACSRSCGMGVSIRVTNQNRRCEMVKQSRLCMVRPCDATKEQADPQEVGTCLRTTQSPEPVHFTFRNCSSVQAFRPRFCGRCSDGRCCTPHATKTAPVTFRCPDGSTLKRPVMFIRTCACHRNCPAKDNVPQRGPETDHASVLTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RLE0</accession>
    <name>A0A2D0RLE0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kintoun</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Dynein assembly factor 2, axonemal</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">dnaaf2</name>
        <name evidence="3" type="synonym">DNAAF2</name>
        <name evidence="3" type="synonym">KTU</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for cytoplasmic pre-assembly of axonemal dyneins, thereby playing a central role in motility in cilia and flagella. Involved in pre-assembly of dynein arm complexes in the cytoplasm before intraflagellar transport loads them for the ciliary compartment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Dynein axonemal particle</location>
        </subcellularLocation>
        <text evidence="3">Localizes in the apical cytoplasm around the gamma-tubulin-positive pericentriolar region, not in the cilia.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIH1 family. Kintoun subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017330966.1">
        <property type="nucleotide sequence ID" value="XM_017475477.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RLE0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000024645"/>
    <dbReference type="GeneID" id="108269534"/>
    <dbReference type="KEGG" id="ipu:108269534"/>
    <dbReference type="CTD" id="55172"/>
    <dbReference type="OrthoDB" id="546764at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120293">
        <property type="term" value="C:dynein axonemal particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070286">
        <property type="term" value="P:axonemal dynein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060285">
        <property type="term" value="P:cilium-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003351">
        <property type="term" value="P:epithelial cilium movement involved in extracellular fluid movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00298">
        <property type="entry name" value="ACD_sHsps_p23-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03069">
        <property type="entry name" value="Kintoun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034727">
        <property type="entry name" value="Kintoun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050734">
        <property type="entry name" value="PIH1/Kintoun_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012981">
        <property type="entry name" value="PIH1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041442">
        <property type="entry name" value="PIH1D1/2/3_CS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22997">
        <property type="entry name" value="PIH1 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22997:SF3">
        <property type="entry name" value="PROTEIN KINTOUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08190">
        <property type="entry name" value="PIH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18201">
        <property type="entry name" value="PIH1_CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIH1 N-terminal" evidence="5">
        <location>
            <begin position="40"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="PIH1D1/2/3 CS-like" evidence="6">
        <location>
            <begin position="249"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="493"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="566"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="493"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="508"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="574"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017330966.1"/>
        </source>
    </evidence>
    <sequence length="642" mass="71636" checksum="CA6364549A21B9FE" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RPH8</accession>
    <name>A0A2D0RPH8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Eukaryotic translation initiation factor 3 subunit E</fullName>
            <shortName evidence="4">eIF3e</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">eif3ea</name>
        <name evidence="4" type="synonym">EIF3E</name>
        <name evidence="4" type="synonym">EIF3S6</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the eIF-3 subunit E family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017332413.1">
        <property type="nucleotide sequence ID" value="XM_017476924.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RPH8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029593"/>
    <dbReference type="GeneID" id="100305109"/>
    <dbReference type="KEGG" id="ipu:100305109"/>
    <dbReference type="CTD" id="325102"/>
    <dbReference type="OMA" id="IMEPNRP"/>
    <dbReference type="OrthoDB" id="417252at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071540">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3e"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21378">
        <property type="entry name" value="eIF3E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03004">
        <property type="entry name" value="eIF3e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016650">
        <property type="entry name" value="eIF3e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019010">
        <property type="entry name" value="eIF3e_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10317">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09440">
        <property type="entry name" value="eIF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21357">
        <property type="entry name" value="EIF3E_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016255">
        <property type="entry name" value="eIF3e_su6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01186">
        <property type="entry name" value="eIF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="6">
        <location>
            <begin position="222"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR016255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017332413.1"/>
        </source>
    </evidence>
    <sequence length="446" mass="52432" checksum="CF14448C52A0ED99" modified="2017-12-20" version="1">MAEYDLTTKIAHFLDRHLVFPLLEFLSVKEIYNETELLHGKLDLLSDTNMVDFAMDVYKSLYLDKDIPNSLREKRTAVVAQLKQLQSETEPIVKMFEDPETTRQMQSTRDGRMLFDYLAEKHAFRQEYLDTLYRYAKFQYECGNYSGAAEYLYFFRVLVPATDRNALNSLWGKLASEILMQNWEAAMEDLTRLRETIDNNTVSSPLQSLQQRTWLIHWSLFVFFNHPKGRDNIIELFLYQPQYLNAIQTMCPHILRYLTTAVITNKDVRKRRQVLKDLVKVIQQESYTYKDPITEFVECLYVNFDFDGAQKKLRECESVLVNDFFLVACLEDFIENARLFIFETFCRIHQCISISMLADKLNMTPEEAERWIVNLIRNARLDAKIDSKLGHVVMGNNAVSPYQQVIEKTKSLSFRSQMLAMNIEKKLSHSNRNETPNWAAQDTSFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RQC3</accession>
    <name>A0A2D0RQC3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutaminyl-peptide cyclotransferase</fullName>
            <ecNumber evidence="4">2.3.2.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Glutaminyl cyclase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Glutaminyl-tRNA cyclotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">qpct</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-terminal L-glutaminyl-[peptide] = N-terminal 5-oxo-L-prolyl-[peptide] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:23652"/>
            <dbReference type="Rhea" id="RHEA-COMP:11736"/>
            <dbReference type="Rhea" id="RHEA-COMP:11846"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:64722"/>
            <dbReference type="ChEBI" id="CHEBI:87215"/>
            <dbReference type="EC" id="2.3.2.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glutaminyl-peptide cyclotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.5" evidence="4"/>
    <dbReference type="RefSeq" id="XP_017332703.1">
        <property type="nucleotide sequence ID" value="XM_017477214.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RQC3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035946"/>
    <dbReference type="GeneID" id="108270471"/>
    <dbReference type="KEGG" id="ipu:108270471"/>
    <dbReference type="CTD" id="25797"/>
    <dbReference type="OrthoDB" id="3907302at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016603">
        <property type="term" value="F:glutaminyl-peptide cyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03880">
        <property type="entry name" value="M28_QC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000029">
        <property type="entry name" value="Glutaminyl-peptide cyclotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037457">
        <property type="entry name" value="M28_QC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040234">
        <property type="entry name" value="QC/QCL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12283">
        <property type="entry name" value="GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12283:SF5">
        <property type="entry name" value="GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M28" evidence="16">
        <location>
            <begin position="120"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017332703.1"/>
        </source>
    </evidence>
    <sequence length="367" mass="41670" checksum="1D0D09492A933DEB" modified="2017-12-20" version="1">MMMYAVRAGTAMRLFALLGIFAIFTGHFCAQIQWTQEKLYHKVSTLSSDDLASVLAQTDIDRMWKNDLKPMLVVRYPGSPGSQAVQQQIKSTLNSLNAGWEVTEDHFQNWTPYGQMPFTNIIATLNPGAKRRLVLSCHYDSKYFPPQWHGREFLGATDSAVPCSMLLEMARAQDKELKTIKDAGSDLTLQLIFFDGEEALYQWTSTDSLYGSRHLATKMENTAHPLGATDTNQLDGIDLFVLLDLIGGPMPRFGNQFSNTARWLSKLQNIERRLHALGQLEDHPNEVQYFWPGMPVGPVQDDHMPFLSRGVRVLHLIPSPFPIVWHTFDDNEENLDRATIQNLNKILQVFVLEYLNMKSQNPLTQAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RQV9</accession>
    <name>A0A2D0RQV9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA (cytosine-5-)-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">dnmt3ab</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-cysteinyl-[protein] + S-adenosyl-L-methionine = S-methyl-L-cysteinyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66544"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:66545"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = a 5-methyl-2'-deoxycytidine in DNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13681"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:11370"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:85454"/>
            <dbReference type="EC" id="2.1.1.37"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:13682"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.37" evidence="2"/>
    <dbReference type="RefSeq" id="XP_017332330.1">
        <property type="nucleotide sequence ID" value="XM_017476841.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RQV9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035135"/>
    <dbReference type="GeneID" id="108270301"/>
    <dbReference type="KEGG" id="ipu:108270301"/>
    <dbReference type="CTD" id="553189"/>
    <dbReference type="OrthoDB" id="641149at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003886">
        <property type="term" value="F:DNA (cytosine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11729">
        <property type="entry name" value="ADDz_Dnmt3a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20154">
        <property type="entry name" value="PWWP_DNMT3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000008">
        <property type="entry name" value="DNA (Cytosine-5)-methyltransferase 3A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000006">
        <property type="entry name" value="DNA (Cytosine-5)-methyltransferase 3B isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000011">
        <property type="entry name" value="DNA methyltransferase 3 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.50">
        <property type="entry name" value="PWWP, helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025766">
        <property type="entry name" value="ADD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044108">
        <property type="entry name" value="ADD_DNMT3A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050390">
        <property type="entry name" value="C5-Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018117">
        <property type="entry name" value="C5_DNA_meth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054724">
        <property type="entry name" value="DNM3A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040552">
        <property type="entry name" value="DNMT3_ADD_GATA1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049554">
        <property type="entry name" value="DNMT3_ADD_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23068:SF10">
        <property type="entry name" value="DNA (CYTOSINE-5)-METHYLTRANSFERASE 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23068">
        <property type="entry name" value="DNA CYTOSINE-5- -METHYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17980">
        <property type="entry name" value="ADD_DNMT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22855">
        <property type="entry name" value="DNM3A_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21255">
        <property type="entry name" value="DNMT3_ADD_GATA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51533">
        <property type="entry name" value="ADD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00094">
        <property type="entry name" value="C5_MTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 14" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6 14" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PWWP" evidence="16">
        <location>
            <begin position="354"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="17">
        <location>
            <begin position="544"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="79"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="253"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="104"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="142"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="282"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="772"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017332330.1"/>
        </source>
    </evidence>
    <sequence length="974" mass="109967" checksum="99E9215F4608B1BA" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RVQ1</accession>
    <name>A0A2D0RVQ1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Neurturin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC108271491</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TGF-beta family. GDNF subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017334574.1">
        <property type="nucleotide sequence ID" value="XM_017479085.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RVQ1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000023613"/>
    <dbReference type="GeneID" id="108271491"/>
    <dbReference type="KEGG" id="ipu:108271491"/>
    <dbReference type="OrthoDB" id="9936891at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030116">
        <property type="term" value="F:glial cell-derived neurotrophic factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043401">
        <property type="entry name" value="GDNF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12173">
        <property type="entry name" value="GDNF SUBFAMILY OF TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12173:SF8">
        <property type="entry name" value="PERSEPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0339">Growth factor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="TGF-beta family profile" evidence="8">
        <location>
            <begin position="112"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017334574.1"/>
        </source>
    </evidence>
    <sequence length="220" mass="25702" checksum="130DA7C810626CBC" modified="2017-12-20" version="1">MRGARAGRRKDGKRWWNQQDPIATSAQCHLTCWKVIFLMSVCLLEIVDGQRLEEKREESHSTTLMAQKEQRRPPWAVLSDPSLAEEGEEYRARWHRSLADISVAYQKSRKERKKRKRQRENLNQDCHLEKKQMRVRDLGLGYDSDEIVLFKYCVGTCMSSRRNYDMALKILTENGSILGRDVSTHPCCRPTRFEAVSFMDTRTNWQTIRWLSAANCSCVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RVY6</accession>
    <name>A0A2D0RVY6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Metabotropic glutamate receptor 4 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC108271537</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017334663.1">
        <property type="nucleotide sequence ID" value="XM_017479174.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RVY6"/>
    <dbReference type="GeneID" id="108271537"/>
    <dbReference type="KEGG" id="ipu:108271537"/>
    <dbReference type="OrthoDB" id="425344at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06376">
        <property type="entry name" value="PBP1_mGluR_groupIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000196">
        <property type="entry name" value="Glutamate metabotropic receptor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000009">
        <property type="entry name" value="Glutamate receptor, metabotropic 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000001">
        <property type="entry name" value="metabotropic glutamate receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000176">
        <property type="entry name" value="metabotropic glutamate receptor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001786">
        <property type="entry name" value="GPCR_3_mGluR4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000162">
        <property type="entry name" value="GPCR_3_mtglu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050726">
        <property type="entry name" value="mGluR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24060">
        <property type="entry name" value="METABOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01054">
        <property type="entry name" value="MTABOTROPC4R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00593">
        <property type="entry name" value="MTABOTROPICR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00979">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00980">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013016978" evidence="14">
        <location>
            <begin position="33"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="590"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="628"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="656"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="696"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="754"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="787"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="829"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="15">
        <location>
            <begin position="590"/>
            <end position="864"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017334663.1"/>
        </source>
    </evidence>
    <sequence length="916" mass="102130" checksum="E1C2766BD185B860" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RW84</accession>
    <name>A0A2D0RW84_ICTPU</name>
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        <submittedName>
            <fullName evidence="8">Odorant receptor 134-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">or90a1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017334763.2">
        <property type="nucleotide sequence ID" value="XM_017479274.2"/>
    </dbReference>
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    <dbReference type="GeneID" id="108271602"/>
    <dbReference type="KEGG" id="ipu:108271602"/>
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    <dbReference type="OrthoDB" id="6359945at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:olfactory receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000096">
        <property type="entry name" value="Odorant receptor 131-2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    </dbReference>
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        <property type="entry name" value="GPCR1_Superfamily_Member"/>
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    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF974">
        <property type="entry name" value="ODORANT RECEPTOR"/>
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        <property type="entry name" value="7tm_1"/>
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    </dbReference>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="190"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="228"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="6">
        <location>
            <begin position="34"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017334763.2"/>
        </source>
    </evidence>
    <sequence length="315" mass="36177" checksum="81D0B2324164ADBF" modified="2023-02-22" version="2">MSMNSTGTLVRDTFSNAFAKNFILVGVWLSLSYINGSVVANFFSNQIFYEDPRYILFIHMVINDAVQLTVTVALFIVSYIYYSINVSVCCVFILVAVFTTRNTPLSLAGMAIERYIAICEPLHHTQICTVQRTYLLIGFIWFICVVPDITDLFITLAMEPISFFHSSVFCLRQNIFKDPVLLYKRQAFDAIYFSLVFLTLVYTYLKVMFAARAISTVKTSAQKARKTILLHGAQLLMCMLSYISPSVEVILNIIFPGHILEIRYANYLIVYILPRFLGPIIYGVRDQKFCKYLKRYFIFVNCKSSTKVDSQEEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S0A3</accession>
    <name>A0A2D0S0A3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Toll-like receptor 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC108272333</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Endomembrane system</location>
            <topology evidence="15">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Toll-like receptor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017336167.1">
        <property type="nucleotide sequence ID" value="XM_017480678.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S0A3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032698"/>
    <dbReference type="GeneID" id="108272333"/>
    <dbReference type="KEGG" id="ipu:108272333"/>
    <dbReference type="OMA" id="LSWNCYF"/>
    <dbReference type="OrthoDB" id="10006997at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:pattern recognition receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032755">
        <property type="term" value="P:positive regulation of interleukin-6 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902533">
        <property type="term" value="P:positive regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000037">
        <property type="entry name" value="Toll-like receptor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47410">
        <property type="entry name" value="TOLL-LIKE RECEPTOR 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47410:SF1">
        <property type="entry name" value="TOLL-LIKE RECEPTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0391">Immunity</keyword>
    <keyword evidence="14" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="818"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="17">
        <location>
            <begin position="867"/>
            <end position="1011"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017336167.1"/>
        </source>
    </evidence>
    <sequence length="1037" mass="119817" checksum="908B6B012F585948" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S5X7</accession>
    <name>A0A2D0S5X7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CST complex subunit TEN1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Protein telomeric pathways with STN1 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Telomere length regulation protein TEN1 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ten1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TEN1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017337625.1">
        <property type="nucleotide sequence ID" value="XM_017482136.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S5X7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021000"/>
    <dbReference type="GeneID" id="108273111"/>
    <dbReference type="KEGG" id="ipu:108273111"/>
    <dbReference type="CTD" id="100134934"/>
    <dbReference type="OrthoDB" id="342190at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990879">
        <property type="term" value="C:CST complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010521">
        <property type="term" value="F:telomerase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032211">
        <property type="term" value="P:negative regulation of telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000203">
        <property type="entry name" value="TEN1 subunit of CST complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029146">
        <property type="entry name" value="Ten1_animal_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33905">
        <property type="entry name" value="CST COMPLEX SUBUNIT TEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33905:SF1">
        <property type="entry name" value="CST COMPLEX SUBUNIT TEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15490">
        <property type="entry name" value="Ten1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0779">Telomere</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017337625.1"/>
        </source>
    </evidence>
    <sequence length="126" mass="14091" checksum="78D98EC790005CB1" modified="2017-12-20" version="1">MVLPAAAVFHFLWEMNSDLVKDGASIRTFGRLTDYLPEESKAILTSHHASVQYQVSIQTTFVEPFDPIIGAQYIVLGELEKSQGDSEAVVRARVFNCVDGVDVALLQKAVNEQRTFFRERNDEADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S8W1</accession>
    <name>A0A2D0S8W1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Zinc finger protein 518A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">si:ch211-214e3.5</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017339184.1">
        <property type="nucleotide sequence ID" value="XM_017483695.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000026514"/>
    <dbReference type="GeneID" id="108273975"/>
    <dbReference type="KEGG" id="ipu:108273975"/>
    <dbReference type="OrthoDB" id="6778897at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379:SF121">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379">
        <property type="entry name" value="KRAB AND ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="230"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="1448"/>
            <end position="1475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="338"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="426"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="484"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="547"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="603"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1289"/>
            <end position="1308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="350"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="447"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="561"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="603"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="628"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="691"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="730"/>
            <end position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017339184.1"/>
        </source>
    </evidence>
    <sequence length="1482" mass="162027" checksum="7EAD4448645E41A3" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SFU0</accession>
    <name>A0A2D0SFU0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">lysoplasmalogenase</fullName>
            <ecNumber evidence="6">3.3.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">tmem86a</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 1-O-(1Z-alkenyl)-sn-glycero-3-phosphocholine + H2O = a 2,3-saturated aldehyde + sn-glycerol 3-phosphocholine</text>
            <dbReference type="Rhea" id="RHEA:22544"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:73359"/>
            <dbReference type="ChEBI" id="CHEBI:77287"/>
            <dbReference type="EC" id="3.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine + H2O = a 2,3-saturated aldehyde + sn-glycero-3-phosphoethanolamine</text>
            <dbReference type="Rhea" id="RHEA:16905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:73359"/>
            <dbReference type="ChEBI" id="CHEBI:77288"/>
            <dbReference type="ChEBI" id="CHEBI:143890"/>
            <dbReference type="EC" id="3.3.2.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM86 family.</text>
    </comment>
    <dbReference type="EC" id="3.3.2.2" evidence="6"/>
    <dbReference type="RefSeq" id="XP_017341584.1">
        <property type="nucleotide sequence ID" value="XM_017486095.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SFU0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008203"/>
    <dbReference type="GeneID" id="108275336"/>
    <dbReference type="KEGG" id="ipu:108275336"/>
    <dbReference type="CTD" id="144110"/>
    <dbReference type="OMA" id="LMFGITH"/>
    <dbReference type="OrthoDB" id="2133758at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047408">
        <property type="term" value="F:alkenylglycerophosphocholine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012506">
        <property type="entry name" value="TMEM86B-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31885">
        <property type="entry name" value="GH04784P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31885:SF10">
        <property type="entry name" value="LYSOPLASMALOGENASE-LIKE PROTEIN TMEM86A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07947">
        <property type="entry name" value="YhhN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="118"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="192"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017341584.1"/>
        </source>
    </evidence>
    <sequence length="284" mass="31588" checksum="DD34CA94E0CC6013" modified="2017-12-20" version="1">MLNCPKQETKTNFYTKEHKIHLCVRLVQCCFCLDIIRSAHGYHPNINMVSPVTVVKSEGPKLVPFFKATCVYFVLWLPTSSPSWFSALIKCLPIFCLWVFLLAHGISFLGAHSSARKILAGLIFSALGDAFLIWQEQGYFSHGLLMFAITHILYSSAFGMKPLNLRAGLVIATISGLCYTLLYPYLSGTFTYLVAVYIALIGFMGWRAIAGVQLANDLWTWTKLSACLGAVLFMVSDLTIAVNKFCFPVPHSRAIIMATYYAAQMLIALSAVECQDADVARKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SJJ7</accession>
    <name>A0A2D0SJJ7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Polynucleotide 5'-hydroxyl-kinase NOL9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15 16 17 18" type="primary">nol9</name>
    </gene>
    <organism evidence="14 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15 16 17" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15 16 17">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Clp1 family. NOL9/GRC3 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017342874.1">
        <property type="nucleotide sequence ID" value="XM_017487385.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017342875.1">
        <property type="nucleotide sequence ID" value="XM_017487386.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017342876.1">
        <property type="nucleotide sequence ID" value="XM_017487387.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017342878.1">
        <property type="nucleotide sequence ID" value="XM_017487389.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SJJ7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000032795"/>
    <dbReference type="GeneID" id="108276069"/>
    <dbReference type="KEGG" id="ipu:108276069"/>
    <dbReference type="CTD" id="79707"/>
    <dbReference type="OMA" id="YFGETSC"/>
    <dbReference type="OrthoDB" id="2405412at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051731">
        <property type="term" value="F:polynucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000448">
        <property type="term" value="P:cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045116">
        <property type="entry name" value="Clp1/Grc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032319">
        <property type="entry name" value="CLP1_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057570">
        <property type="entry name" value="NOL9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057573">
        <property type="entry name" value="NOL9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755">
        <property type="entry name" value="CLEAVAGE/POLYADENYLATION FACTOR IA SUBUNIT CLP1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755:SF3">
        <property type="entry name" value="POLYNUCLEOTIDE 5'-HYDROXYL-KINASE NOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16575">
        <property type="entry name" value="CLP1_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24419">
        <property type="entry name" value="Cupin_NOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25467">
        <property type="entry name" value="NOL9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NOL9 N-terminal" evidence="12">
        <location>
            <begin position="151"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Clp1 P-loop" evidence="11">
        <location>
            <begin position="345"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="NOL9 C-terminal" evidence="13">
        <location>
            <begin position="571"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="77"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF24419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF25467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017342874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017342875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017342876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017342878.1"/>
        </source>
    </evidence>
    <sequence length="719" mass="78696" checksum="B0AB215D9DD1B5A9" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SKI6</accession>
    <name>A0A2D0SKI6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Calcium/calmodulin-dependent protein kinase IGb</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">camk1gb</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017343212.1">
        <property type="nucleotide sequence ID" value="XM_017487723.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SKI6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000028044"/>
    <dbReference type="GeneID" id="108276228"/>
    <dbReference type="KEGG" id="ipu:108276228"/>
    <dbReference type="CTD" id="393802"/>
    <dbReference type="OMA" id="YAWKKNT"/>
    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000026">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000453">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type 1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5011977066" evidence="9">
        <location>
            <begin position="18"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="71"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="465"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="473"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017343212.1"/>
        </source>
    </evidence>
    <sequence length="484" mass="54839" checksum="1EA3392AC691C97E" modified="2017-12-20" version="1">MFVSLRSAHQLLRLSRAVLCPCCPCCPCLPILNTWSSYIPEEIPGETSAEMGRKEADYDWKKSTDNIQEVFDFIEVLGSGAFSEVFMVKERKTGKLFAIKCVKKKNKRDISLENEIAVLRRIKHENVVGLEDLYESRTHYYLVMQLVSGGELFDRILDRGVYSEKDASLVIRQVLEGVNYLHKNGIVHRDLKPENLLYYSPDEESKIMISDFGLSKMEDKGIMSTACGTPGYVAPEVLAQKPYSKAVDCWSIGVITYILLCGYPPFYEESETRLFLKIMKAQYEFDSPFWDDISESAKDFIRNMMKKNPNLRYTTEQALRHPWIIGKTAQSRDIYHSVSEQIQKNFAKSKWKQAFNATVAINHMKKLQLAHGEASVKQSQTKTQLPDIKVIATSHLEATDNNPVHDILEPSGNLPCSHMTLPSVSMETKTQYLLPKVSHSVTLTVNEKTKHVYHSEPGNLNGYEKTGSHSGEKLQTSAGVCSVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SLR6</accession>
    <name>A0A2D0SLR6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Carbohydrate sulfotransferase</fullName>
            <ecNumber evidence="11">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">si:ch211-236h17.3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Golgi apparatus membrane</location>
            <topology evidence="1 11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sulfotransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_017343446.1">
        <property type="nucleotide sequence ID" value="XM_017487957.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SLR6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000025742"/>
    <dbReference type="GeneID" id="108276348"/>
    <dbReference type="KEGG" id="ipu:108276348"/>
    <dbReference type="OrthoDB" id="2019940at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030166">
        <property type="term" value="P:proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018011">
        <property type="entry name" value="Carb_sulfotrans_8-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137:SF60">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9 11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017343446.1"/>
        </source>
    </evidence>
    <sequence length="361" mass="42293" checksum="905FC911B6983846" modified="2017-12-20" version="1">MMMVMKKKMKSRIAMMKKMVMMVKPRAGRVLLATCAGSFFILILYFQNSARAGTEHLLGRTGVPGKLRRTPLQTLIDGDQWDHSAVRTTLQARRSFLDEACHSHTRKRRVLSPEDLRHLLVDDRHGLLYCYVPKVACTNWKRTMMVLTGDGRYRDPLAIPANEAHAPGRLRSLSEYSKAEINRRLRTYLKFIFVRDPFERLVSAYRNKFTRSYNTAFHKRYGTKIVRRHRANPRPEALEKGSDVSFEEFVYYLVDPRTQREEPFNEHWERAHTLCHPCLIHYDVVGKYESLAQDSRYVLKLAGAEGEVEFPASSKSTRTTESMAAKFFNNISPFYQKKLYNLYRMDFLLFNYSTPTYLKFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SMG1</accession>
    <name>A0A2D0SMG1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Opsin 4xa</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">opn4xa</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Photoreceptor implicated in non-image-forming responses to light.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017343894.2">
        <property type="nucleotide sequence ID" value="XM_017488405.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SMG1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000006791"/>
    <dbReference type="GeneID" id="108276607"/>
    <dbReference type="KEGG" id="ipu:108276607"/>
    <dbReference type="CTD" id="100331502"/>
    <dbReference type="OrthoDB" id="9996086at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000044">
        <property type="entry name" value="Opsin, ultraviolet-sensitive"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050125">
        <property type="entry name" value="GPCR_opsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001760">
        <property type="entry name" value="Opsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027430">
        <property type="entry name" value="Retinal_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24240">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00238">
        <property type="entry name" value="OPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00238">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0157">Chromophore</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 14" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="11 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0681">Retinal protein</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="54"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="181"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="242"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="33"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="411"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017343894.2"/>
        </source>
    </evidence>
    <sequence length="431" mass="48070" checksum="2FE91ADB86E25865" modified="2023-06-28" version="2">MDRGFYRKVDVPDHVHYITASIVAVIGTVGVIGNALVIYAFFCNKKLRSPPNYFIINLAVSDLLMVSTQSPMFFVNSLYKEWVFGKTGCEIYAFCSALFGITSMINLVAISIDRYIVITKPLQAIGWNSKRRTLISILIVWIYSLAWSSAPLLGWSSYVPEGFMTSCTWDYVTSTPANKSYTLMLCCLVFFVPLGIISYCYVFMFLAIRNTSRDVESLGTQMRSTTLVQQQSIKREWKLAKVAFVVIVVFVLSWSPYAFVTLIAWAGYSSILTPYSNAVPAIIAKASAIYNPFIYAIIHSKYRDTLAEQVPCLHFLNKRTRKQCVPGSNSGFSLRNSVLSVQSSGSKTKFHSVSSVLTGHTVCSGVMLDPLAQQAPLLSSSQSANQLTEEEQRHQLRLEINFKDFLSQEKPAISSQSLPGHSGNDLLEDTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0ST49</accession>
    <name>A0A2D0ST49_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">Apoptosis inducing factor mitochondria associated 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">aifm4</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11 12">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017345668.1">
        <property type="nucleotide sequence ID" value="XM_017490179.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017345669.1">
        <property type="nucleotide sequence ID" value="XM_017490180.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0ST49"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007281"/>
    <dbReference type="GeneID" id="108277429"/>
    <dbReference type="KEGG" id="ipu:108277429"/>
    <dbReference type="CTD" id="791218"/>
    <dbReference type="GeneTree" id="ENSGT00940000164941"/>
    <dbReference type="OMA" id="EEHCMAR"/>
    <dbReference type="OrthoDB" id="432169at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03478">
        <property type="entry name" value="Rieske_AIFL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.102.10.10:FF:000003">
        <property type="entry name" value="apoptosis-inducing factor 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050446">
        <property type="entry name" value="FAD-oxidoreductase/Apoptosis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028202">
        <property type="entry name" value="Reductase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43557">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43557:SF7">
        <property type="entry name" value="RIESKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14759">
        <property type="entry name" value="Reductase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="9">
        <location>
            <begin position="11"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017345668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017345669.1"/>
        </source>
    </evidence>
    <sequence length="530" mass="58677" checksum="391E15ABF8B616BE" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SWU1</accession>
    <name>A0A2D0SWU1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="20 21">Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase</fullName>
            <shortName evidence="21">GNT-I</shortName>
            <shortName evidence="21">GlcNAc-T I</shortName>
            <ecNumber evidence="17 21">2.4.1.101</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18 21">N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">mgat1a</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16 21">Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 21">
            <text>N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + UDP-N-acetyl-alpha-D-glucosamine = N(4)-{beta-D-GlcNAc-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11456"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="Rhea" id="RHEA-COMP:14368"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:60625"/>
            <dbReference type="EC" id="2.4.1.101"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="21">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="21">The cofactor is mostly bound to the substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 21">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 21">Golgi apparatus membrane</location>
            <topology evidence="1 21">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 21">Belongs to the glycosyltransferase 13 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.101" evidence="17 21"/>
    <dbReference type="RefSeq" id="XP_017346996.1">
        <property type="nucleotide sequence ID" value="XM_017491507.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SWU1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000001400"/>
    <dbReference type="GeneID" id="108278272"/>
    <dbReference type="KEGG" id="ipu:108278272"/>
    <dbReference type="CTD" id="393649"/>
    <dbReference type="OMA" id="DSFKMLA"/>
    <dbReference type="OrthoDB" id="440755at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003827">
        <property type="term" value="F:alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02514">
        <property type="entry name" value="GT13_GLCNAC-TI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.180.20:FF:000001">
        <property type="entry name" value="alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000055">
        <property type="entry name" value="Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.20">
        <property type="entry name" value="N-Acetylglucosaminyltransferase I, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004139">
        <property type="entry name" value="Glyco_trans_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052261">
        <property type="entry name" value="Glycosyltransferase_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10468:SF0">
        <property type="entry name" value="ALPHA-1,3-MANNOSYL-GLYCOPROTEIN 2-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10468">
        <property type="entry name" value="PROTEIN O-LINKED-MANNOSE BETA-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE 1/ALPHA-1,3-MANNOSYL-GLYCOPROTEIN 2-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03071">
        <property type="entry name" value="GNT-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 21" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12 21" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15 21" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 21" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00037706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00038949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00041712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00069743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU368119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017346996.1"/>
        </source>
    </evidence>
    <sequence length="454" mass="51846" checksum="D4C031F4C95D6C51" modified="2017-12-20" version="1">MFHKKGCLIICGALLFIAWNALLIFVLLGWSPEGGVSISAEKDPQNSNTVEKLLQLISAFETELESQNKILLQIQSHKSLWEEQRKKPTAFRSKLDIKSHFTDAGSNSQSSPVVIPILVMACNRVTVSRCLDKLLEYRPSPELFPIIVSQDCGHTQTADVIASYGSKLTHIKQPDLSEISVPAGHNKFQGYYKIARHYRWALNQVFNTFSYSAVIVVEDDLEVAPDFFEYFKALHPVLKSDQTLWCVSAWNDNGRNGYVDPAKSELLYRTDFFPGLGWMLLKEIWIELEPKWPASFWDDWMRHPNQRKGRSCIRPEISRTLTFGRKGVSLGQFYDKYLRFIKLNTEFVPFTSRDLSYLEQGKYDQAFEKEVYSAPLVTAVELKNGKLYGPGPFRLQYSNAQSFKTLARDLGLMDDLKSGVPRAGYRGVVSFFSQGKRIYIAPPVGWTKYDPSWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SY47</accession>
    <name>A0A2D0SY47_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transmembrane protein 205</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">tmem205</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM205 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347609.1">
        <property type="nucleotide sequence ID" value="XM_017492120.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SY47"/>
    <dbReference type="GeneID" id="108278656"/>
    <dbReference type="KEGG" id="ipu:108278656"/>
    <dbReference type="CTD" id="374882"/>
    <dbReference type="OrthoDB" id="1641132at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042623">
        <property type="entry name" value="TMEM205"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025423">
        <property type="entry name" value="TMEM205-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46916">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46916:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13664">
        <property type="entry name" value="DUF4149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="TMEM205-like" evidence="8">
        <location>
            <begin position="44"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017347609.1"/>
        </source>
    </evidence>
    <sequence length="215" mass="24461" checksum="FA65BD041E07FCA7" modified="2017-12-20" version="1">MALYGLKLCEATHHEVGPFETMNTKFRMATEGDPAGMVKVLHLLLMAFSWGMQLWVSFIAGYVLLSKVTRHTFGLVQSKLFPMYFYCLLGSNTVSLAIYAVYHPRELLNWHETIQMALFFVAVIVAGLNAQWFTPAVIEHLLVMMEIEQEHGLGGEIGFSTNRERYIKLREQDPKYKAHRNSFNRFHGISTLSNLIGFACTTINLIYLALHLASI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SY87</accession>
    <name>A0A2D0SY87_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription elongation factor 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">elof1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Factor involved in transcription-coupled nucleotide excision repair (TC-NER), a mechanism that rapidly removes RNA polymerase II-blocking lesions from the transcribed strand of active genes. Acts as a key adapter required to anchor TC-NER factors to RNA polymerase II: stably positions UVSSA and the DCX(ERCC8) complex (also named CSA complex) on arrested RNA polymerase II, leading to neddylation and activation of the DCX(ERCC8) complex and ubiquitination of RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Associates with RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ELOF1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347637.1">
        <property type="nucleotide sequence ID" value="XM_017492148.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SY87"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000010173"/>
    <dbReference type="GeneID" id="108278668"/>
    <dbReference type="KEGG" id="ipu:108278668"/>
    <dbReference type="CTD" id="84337"/>
    <dbReference type="OMA" id="CLDANKK"/>
    <dbReference type="OrthoDB" id="445983at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.190:FF:000002">
        <property type="entry name" value="Transcription elongation factor 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007808">
        <property type="entry name" value="Elf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038567">
        <property type="entry name" value="T_Elf1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934:SF0">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05129">
        <property type="entry name" value="Zn_ribbon_Elf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017347637.1"/>
        </source>
    </evidence>
    <sequence length="125" mass="14448" checksum="A43391E2F15BE791" modified="2017-12-20" version="1">MALYNTVIVCTCLQKKFSGRFSCVYSRCTCSLYIQNRVIRYQMGRRKSKRKPPPKKKMTGNLDTQFTCPFCNHEKSCDVKMERSRNTGIISCTVCLEEFQTPITYLSEPVDVYSDWIDACEAANQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SZ47</accession>
    <name>A0A2D0SZ47_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Splicing factor, arginine/serine-rich 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">srsf1b</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">May play a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the splicing factor SR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347806.1">
        <property type="nucleotide sequence ID" value="XM_017492317.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SZ47"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000012841"/>
    <dbReference type="GeneID" id="108278759"/>
    <dbReference type="KEGG" id="ipu:108278759"/>
    <dbReference type="CTD" id="393565"/>
    <dbReference type="OMA" id="PREPAYP"/>
    <dbReference type="OrthoDB" id="1099063at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12597">
        <property type="entry name" value="RRM1_SRSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12767">
        <property type="entry name" value="RRM2_SRSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000053">
        <property type="entry name" value="Serine/arginine-rich splicing factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000170">
        <property type="entry name" value="Serine/arginine-rich splicing factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034520">
        <property type="entry name" value="SRSF1_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029538">
        <property type="entry name" value="SRSF1_RRM2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF66">
        <property type="entry name" value="SERINE_ARGININE-RICH SPLICING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="16"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="120"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="90"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="192"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="92"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="204"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017347806.1"/>
        </source>
    </evidence>
    <sequence length="245" mass="27477" checksum="4255B835734F5039" modified="2017-12-20" version="1">MSSGGVIRGPAGNNDCRIYVGNLPPDIRTKDVEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFPRSGRGGGRGGGGGGAGAPRGRYGPPSRRSEYRVIVSGLPPSGSWQDLKDHMREAGDVCYADVFRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVDGPRSPSYGRSRSRSRSRSRSRSNSRSRSYSPRRSRGSPRYSPRHSRSRSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T3H1</accession>
    <name>A0A2D0T3H1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Origin recognition complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">orc5</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ORC5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017349462.1">
        <property type="nucleotide sequence ID" value="XM_017493973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T3H1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000036137"/>
    <dbReference type="GeneID" id="108279644"/>
    <dbReference type="KEGG" id="ipu:108279644"/>
    <dbReference type="CTD" id="5001"/>
    <dbReference type="OMA" id="QLRRWHG"/>
    <dbReference type="OrthoDB" id="365981at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000673">
        <property type="entry name" value="Origin recognition complex subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020796">
        <property type="entry name" value="ORC5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047088">
        <property type="entry name" value="ORC5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048866">
        <property type="entry name" value="ORC5_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12705">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12705:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14630">
        <property type="entry name" value="ORC5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21639">
        <property type="entry name" value="ORC5_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orc1-like AAA ATPase" evidence="10">
        <location>
            <begin position="23"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="ORC5 lid" evidence="12">
        <location>
            <begin position="214"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Origin recognition complex subunit 5 C-terminal" evidence="11">
        <location>
            <begin position="312"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017349462.1"/>
        </source>
    </evidence>
    <sequence length="445" mass="50774" checksum="DCB0672C9EF0D27D" modified="2017-12-20" version="1">MSGAVLQCPGYPEDKLRTLTEDLTCREVQAGTLLSLMGEPDQYSYPSVFIYGHRATGKSHVLLTLLTQLQLPYAAVSCVECVSAGLMLEQVFSALFGPDAASLLPRSPSLSDFVRIYRQACVERPARQTRYIVVEKAELLRDMEANLLPALLRLQELVDDNVTVFLLSEIVWEKFRPNTGCFEPLLLHFPDYTKAELLHILSRDAHPSHTPELYTSYINILLGVFYSVCRDLRELRHLAALNFCKFCEPLEKGEARESDTHKLWKNIEPHLKKAMQSVYLREVSSVQWEQQQMDEKEATALRGLSAHAHVELPYYSKFLLIAAYLASYNPARTDRRFFLKHHGKIKKTNFLKKHEKTSNHLLGPKPFPLDRLLAIFYSVVDSRVAPTANIFSQISSLVTLQLLTQVGHDDQLDCPKYKCAVSLDFILAISRTLSFDIVRYLYDFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T3R9</accession>
    <name>A0A2D0T3R9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10 11">Iroquois-class homeodomain protein IRX-2a isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">irx2a</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/IRO homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017349466.1">
        <property type="nucleotide sequence ID" value="XM_017493977.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017349554.1">
        <property type="nucleotide sequence ID" value="XM_017494065.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T3R9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000000093"/>
    <dbReference type="GeneID" id="108279631"/>
    <dbReference type="KEGG" id="ipu:108279631"/>
    <dbReference type="CTD" id="394032"/>
    <dbReference type="GeneTree" id="ENSGT00940000161198"/>
    <dbReference type="OMA" id="HPHEDAS"/>
    <dbReference type="OrthoDB" id="5399138at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000003">
        <property type="entry name" value="Iroquois-class homeobox protein IRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003893">
        <property type="entry name" value="Iroquois_homeo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211">
        <property type="entry name" value="IROQUOIS-CLASS HOMEODOMAIN PROTEIN IRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211:SF15">
        <property type="entry name" value="IROQUOIS-CLASS HOMEODOMAIN PROTEIN IRX-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00548">
        <property type="entry name" value="IRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="120"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="122"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="185"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="262"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="33"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="262"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017349466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017349554.1"/>
        </source>
    </evidence>
    <sequence length="445" mass="47684" checksum="00B5F87A4034F61A" modified="2017-12-20" version="1">MSYPQGYLYQPGPGSLALYPLRSEELELAARCSSSSSSSSSSSSSSSSTGSAFTPYPASAAFTASAAGGFASALSYHTDPATGFPSYMASPYDPPPSAMAAPLGFPPYGSAGFPPSPYQLNDPAYRKNATRDATSTLKAWLNEHRKNPYPTKGEKIMLAIITKMTLTQVSTWFANARRRLKKENKVTWGARAKSEDEDEEEGVRAERKDEEDEEDDKHADNGDASAEDEGISLHVDSLTDHSAESDGEKVLCRIDDFSCKPAPEAKEKRSEIDLASGHGGHGGLSPKPMTSSPLTGVEAPLLDAHRDKNKTCADVQSQNTKPKLWSLAEIATSDPKQPLQSQNCPSGGGIWTSGQSSVAPSASILARPIYYTSPFYGNYANYGNFGQGILRYGSLHKHAPETPMLKANHANHVQTEQHFRALSAEAKKADSGDVCTLGPQSYLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T4X9</accession>
    <name>A0A2D0T4X9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Podocalyxin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Podocalyxin-like protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">podxl</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell projection</location>
            <location evidence="6">Filopodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cell projection</location>
            <location evidence="7">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Microvillus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Ruffle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane raft</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the podocalyxin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017349665.2">
        <property type="nucleotide sequence ID" value="XM_017494176.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T4X9"/>
    <dbReference type="GeneID" id="108279745"/>
    <dbReference type="KEGG" id="ipu:108279745"/>
    <dbReference type="CTD" id="5420"/>
    <dbReference type="OrthoDB" id="9948358at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030175">
        <property type="term" value="C:filopodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031528">
        <property type="term" value="C:microvillus membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022408">
        <property type="term" value="P:negative regulation of cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033634">
        <property type="term" value="P:positive regulation of cell-cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032534">
        <property type="term" value="P:regulation of microvillus assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013836">
        <property type="entry name" value="CD34/Podocalyxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017403">
        <property type="entry name" value="PODXL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12067">
        <property type="entry name" value="PODOCALYXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12067:SF5">
        <property type="entry name" value="PODOCALYXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06365">
        <property type="entry name" value="CD34_antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0966">Cell projection</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 21" id="KW-0732">Signal</keyword>
    <keyword evidence="11 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039954656" description="Podocalyxin" evidence="21">
        <location>
            <begin position="24"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="451"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="23"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="116"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="24"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="138"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="195"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="243"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="315"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="342"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00007029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00017371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_017349665.2"/>
        </source>
    </evidence>
    <sequence length="548" mass="57430" checksum="622726E07EDA964D" modified="2023-06-28" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T6R9</accession>
    <name>A0A2D0T6R9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cadherin-12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">cdh12a</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Cadherins are calcium-dependent cell adhesion proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017350583.1">
        <property type="nucleotide sequence ID" value="XM_017495094.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T6R9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000002924"/>
    <dbReference type="GeneID" id="108280247"/>
    <dbReference type="KEGG" id="ipu:108280247"/>
    <dbReference type="CTD" id="568508"/>
    <dbReference type="OMA" id="VIEDIGH"/>
    <dbReference type="OrthoDB" id="6250271at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000008">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000009">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000012">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000014">
        <property type="entry name" value="Cadherin 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000097">
        <property type="entry name" value="cadherin-12 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000233">
        <property type="entry name" value="Cadherin_Y-type_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF96">
        <property type="entry name" value="CADHERIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01049">
        <property type="entry name" value="CADH_Y-type_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 13 14" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 14" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 18" id="KW-0732">Signal</keyword>
    <keyword evidence="3 14 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013402116" description="Cadherin-12" evidence="18">
        <location>
            <begin position="21"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="610"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="19">
        <location>
            <begin position="76"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="19">
        <location>
            <begin position="157"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="19">
        <location>
            <begin position="266"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="19">
        <location>
            <begin position="381"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="19">
        <location>
            <begin position="484"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="767"/>
            <end position="787"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017350583.1"/>
        </source>
    </evidence>
    <sequence length="787" mass="86955" checksum="1DF6066B745C385D" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T6U3</accession>
    <name>A0A2D0T6U3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Neuritin 1b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">nrn1b</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the neuritin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017350612.1">
        <property type="nucleotide sequence ID" value="XM_017495123.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T6U3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000004815"/>
    <dbReference type="GeneID" id="108280273"/>
    <dbReference type="KEGG" id="ipu:108280273"/>
    <dbReference type="CTD" id="449839"/>
    <dbReference type="OMA" id="CQDEAVE"/>
    <dbReference type="OrthoDB" id="9928047at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990138">
        <property type="term" value="P:neuron projection extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026144">
        <property type="entry name" value="Neuritin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15902:SF1">
        <property type="entry name" value="NEURITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15902">
        <property type="entry name" value="NEURITIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15056">
        <property type="entry name" value="NRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012926252" evidence="9">
        <location>
            <begin position="28"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017350612.1"/>
        </source>
    </evidence>
    <sequence length="143" mass="15505" checksum="7C3E405501A33B4D" modified="2017-12-20" version="1">MGFTLTDRHGSLLCLLQLASLLRAVSSAGACETVFKGFSDCLLSLGGNMLNHPQDLDDLENLDTVCSYWSEFLLCTSAAVAGCQDEAVELWEKLKAPSGSLNYKGSLFELCSKDNGASGLGFPLELIVWAMTLSKLVMWVIFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0TAZ8</accession>
    <name>A0A2D0TAZ8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Selenoprotein Pb</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">selenop2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017351845.1">
        <property type="nucleotide sequence ID" value="XM_017496356.3"/>
    </dbReference>
    <dbReference type="STRING" id="7998.ENSIPUP00000003816"/>
    <dbReference type="GeneID" id="108280874"/>
    <dbReference type="KEGG" id="ipu:108280874"/>
    <dbReference type="CTD" id="791479"/>
    <dbReference type="OrthoDB" id="6134775at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008430">
        <property type="term" value="F:selenium binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001887">
        <property type="term" value="P:selenium compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007671">
        <property type="entry name" value="Selenoprotein-P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037941">
        <property type="entry name" value="SeP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10105">
        <property type="entry name" value="SELENOPROTEIN P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10105:SF4">
        <property type="entry name" value="SELENOPROTEIN P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04592">
        <property type="entry name" value="SelP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0711">Selenium</keyword>
    <keyword evidence="4 10" id="KW-0712">Selenocysteine</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012338810" evidence="7">
        <location>
            <begin position="20"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Selenoprotein P N-terminal" evidence="8">
        <location>
            <begin position="29"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="188"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="188"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="233"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="253"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="10">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017351845.1"/>
        </source>
    </evidence>
    <sequence length="265" mass="30090" checksum="454478C899F863B7" modified="2017-12-20" version="1">MFRALSALCLASLPSLLWTSPLFVERDNNQSTVCKLAPLWDIDGYSPMNELLGNVVVVALLKASUHFCLTQAAKLGDLRDKLVRGNLTGVAFLIVNEQDAHSRAMYWEMKRRTSEGIPVYQQSPLQSNVWDTLQGDKDDFLVYDRCGRLTFHIMLPFSILHYPYVEAAVRATYFQNICNCTLDSNSTRLTSSNVTRGKSFEQNPTTAESPNINNHHHHDHDHHVRHDHSRNRPSQYPAVTQTTSTAPVKKHAHPDPERVNHMNTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979E235</accession>
    <name>A0A979E235_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein si:dkey-246e1.3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">si:dkey-246e1.3</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047007428.2">
        <property type="nucleotide sequence ID" value="XM_047151472.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979E235"/>
    <dbReference type="GeneID" id="124626404"/>
    <dbReference type="KEGG" id="ipu:124626404"/>
    <dbReference type="OrthoDB" id="8943878at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_047007428.2"/>
        </source>
    </evidence>
    <sequence length="160" mass="18216" checksum="9364995ADB7FDA93" modified="2023-06-28" version="2">MSARPRTLRSSALYLNSTNGDGESRDVVYECKVYNISMLSLSVCVLTVTAVVCCVSYLSRWRHRKRAREYESAVDCNLEEEQGNLKVMQRSQSLRKALPGFQPAKSFRKDDSSIYYIYTNPLPVGRHDEDDDVEATHTTLTVNDPKSSIILDPPIFYLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EGZ5</accession>
    <name>A0A979EGZ5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Uridine-cytidine kinase</fullName>
            <ecNumber evidence="17">2.7.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">si:ch211-243j20.2</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">May contribute to UTP accumulation needed for blast transformation and proliferation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 17">
            <text>cytidine + ATP = CMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 17">
            <text>uridine + ATP = UMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16825"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="17">Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with RNF19B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the uridine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.48" evidence="17"/>
    <dbReference type="RefSeq" id="XP_047008022.1">
        <property type="nucleotide sequence ID" value="XM_047152066.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EGZ5"/>
    <dbReference type="GeneID" id="108279217"/>
    <dbReference type="OrthoDB" id="10257085at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004849">
        <property type="term" value="F:uridine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008655">
        <property type="term" value="P:pyrimidine-containing compound salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02023">
        <property type="entry name" value="UMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000010">
        <property type="entry name" value="Uridine-cytidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000200">
        <property type="entry name" value="Uridine-cytidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000764">
        <property type="entry name" value="Uridine_kinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001097">
        <property type="entry name" value="PRK00129.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004018">
        <property type="entry name" value="PRK05480.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00235">
        <property type="entry name" value="udk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00988">
        <property type="entry name" value="URIDINKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="19">
        <location>
            <begin position="104"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="20">
        <location>
            <begin position="334"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00056790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00065923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_047008022.1"/>
        </source>
    </evidence>
    <sequence length="552" mass="62235" checksum="344A412BB7C62822" modified="2023-02-22" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979ENX7</accession>
    <name>A0A979ENX7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Peptide chain release factor 1-like, mitochondrial isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mtrf1l</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047010359.1">
        <property type="nucleotide sequence ID" value="XM_047154403.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979ENX7"/>
    <dbReference type="GeneID" id="108263755"/>
    <dbReference type="CTD" id="54516"/>
    <dbReference type="OrthoDB" id="2019491at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070126">
        <property type="term" value="P:mitochondrial translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000004">
        <property type="entry name" value="Peptide chain release factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1660:FF:000011">
        <property type="entry name" value="Peptide chain release factor 1-like, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050057">
        <property type="entry name" value="Prokaryotic/Mito_RF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43804">
        <property type="entry name" value="LD18447P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43804:SF3">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR 1-LIKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="Release factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prokaryotic-type class I peptide chain release factors" evidence="3">
        <location>
            <begin position="306"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_047010359.1"/>
        </source>
    </evidence>
    <sequence length="442" mass="50226" checksum="4E1A6499822E36D0" modified="2023-02-22" version="1">MAVACFTKQLLRCVVFKPIGKRARAAVRTVSTHALRHLTHSRARETPVYRVSRCCRDFHTTNSAMVAKIMTADEMFSTKSLQDYLRTLQTEYNECLRDVNSTELQTDDEEELRAKRRKVTALEPLVQKIRDLESKQRDFEETESLLKENDPELRELAETEREHCLASIQEMKQTILSLLVPEEESDMSDLILEVTAGVGGQEAMLFTAEIFDMYQNYAGFHGWGFDTLEYMPSDIGGVRHAAASISGPLSYKKMKFEAGVHRVQRVPKTEKQGRMHTSTMTVVILPQPAEISFTINPKDLRIETKRASGAGGQHVNTTDSAVRIVHLPTGTVAECQQERSQIKNKEKAMKLLRAKLYSAKLEEETSKRYQARKLQVFPGQPGDIIPPACPGLVSGSLPSGVCLVELHRHPPGWHPYQMPRPPQLASRYLEKWRLYYEAFLDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979F2J5</accession>
    <name>A0A979F2J5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18 19 20" type="primary">herc1</name>
    </gene>
    <organism evidence="17 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="17">SDA103</strain>
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
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        <alternativeName>
            <fullName evidence="20">Adenylate cyclase-inhibiting G alpha protein</fullName>
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        <name evidence="24" type="primary">gnai2b</name>
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        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
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    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-alpha family. G(i/o/t/z) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_047014633.1">
        <property type="nucleotide sequence ID" value="XM_047158677.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979F3K6"/>
    <dbReference type="GeneID" id="108271854"/>
    <dbReference type="CTD" id="327650"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007193">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001973">
        <property type="term" value="P:G protein-coupled adenosine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide-binding protein G(I) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002487">
        <property type="entry name" value="Guanine nucleotide-binding protein G(i) subunit alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001408">
        <property type="entry name" value="Gprotein_alpha_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF73">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN G(I) SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00441">
        <property type="entry name" value="GPROTEINAI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11 21" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10 22" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0519">Myristate</keyword>
    <keyword evidence="9 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0564">Palmitate</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="99"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="124"/>
            <end position="130"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="149"/>
            <end position="153"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="218"/>
            <end position="221"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00037181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00039891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00042569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_047014633.1"/>
        </source>
    </evidence>
    <sequence length="303" mass="35007" checksum="41E9255CAFF1A2DC" modified="2023-02-22" version="1">MKIIHEDGYSEDECKQYRAVVYSNTIQSIMAIIKAMSNLKIDYGDPARTDDARQLFALSAAAEEQGILPDDLSNVIRRLWTDSGVQGCFSRSREYQLNDSAAYYLNDLERIARADYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSAYDLMLAEDEEMNRMHESMKLFDSICNNKWFTETSIILFLNKKDLFEEKITRSPLSICFPEYTGANKYDEAASYIQTKFEDLNKKKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7R0S5</accession>
    <name>A0A9F7R0S5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutaminase</fullName>
            <ecNumber evidence="3">3.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">glsb</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_053536705.1">
        <property type="nucleotide sequence ID" value="XM_053680730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7R0S5"/>
    <dbReference type="GeneID" id="108266312"/>
    <dbReference type="CTD" id="564746"/>
    <dbReference type="OrthoDB" id="9995210at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006543">
        <property type="term" value="P:L-glutamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.210:FF:000001">
        <property type="entry name" value="Glutaminase kidney isoform, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.710.10:FF:000002">
        <property type="entry name" value="glutaminase kidney isoform, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000019">
        <property type="entry name" value="Glutaminase liver isoform, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00313">
        <property type="entry name" value="Glutaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015868">
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        <property type="entry name" value="Ank_2"/>
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        <property type="entry name" value="EF-hand_14"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Glutaminase"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
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    <keyword evidence="8 11" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
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        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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            <taxon>Ostariophysi</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
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        <scope>IDENTIFICATION</scope>
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    </feature>
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    </evidence>
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        <source>
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            <dbReference type="PROSITE" id="PS50994"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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        <source>
            <dbReference type="RefSeq" id="XP_053536946.1"/>
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        <source>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
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                <person name="Liu S."/>
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                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
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                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
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    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="6">Blood</tissue>
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        <property type="project" value="UniProtKB-KW"/>
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    <feature type="domain" description="Galectin" evidence="4">
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    <feature type="domain" description="Galectin" evidence="4">
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    <feature type="domain" description="Galectin" evidence="4">
        <location>
            <begin position="577"/>
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    <feature type="domain" description="Galectin" evidence="4">
        <location>
            <begin position="749"/>
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    </feature>
    <feature type="domain" description="Galectin" evidence="4">
        <location>
            <begin position="919"/>
            <end position="1054"/>
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    </feature>
    <feature type="domain" description="Galectin" evidence="4">
        <location>
            <begin position="1091"/>
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    <feature type="domain" description="Galectin" evidence="4">
        <location>
            <begin position="1261"/>
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    <feature type="domain" description="Galectin" evidence="4">
        <location>
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    <feature type="domain" description="Galectin" evidence="4">
        <location>
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    <feature type="domain" description="Galectin" evidence="4">
        <location>
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    <feature type="domain" description="Galectin" evidence="4">
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    <feature type="domain" description="Galectin" evidence="4">
        <location>
            <begin position="2287"/>
            <end position="2422"/>
        </location>
    </feature>
    <feature type="domain" description="Galectin" evidence="4">
        <location>
            <begin position="2459"/>
            <end position="2589"/>
        </location>
    </feature>
    <feature type="domain" description="Galectin" evidence="4">
        <location>
            <begin position="2631"/>
            <end position="2766"/>
        </location>
    </feature>
    <feature type="domain" description="Galectin" evidence="4">
        <location>
            <begin position="2798"/>
            <end position="2933"/>
        </location>
    </feature>
    <feature type="domain" description="Galectin" evidence="4">
        <location>
            <begin position="2965"/>
            <end position="3100"/>
        </location>
    </feature>
    <feature type="domain" description="Galectin" evidence="4">
        <location>
            <begin position="3137"/>
            <end position="3266"/>
        </location>
    </feature>
    <feature type="domain" description="Galectin" evidence="4">
        <location>
            <begin position="3307"/>
            <end position="3434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_053533827.1"/>
        </source>
    </evidence>
    <sequence length="3439" mass="378513" checksum="3D3E4508F3367542" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RG70</accession>
    <name>A0A9F7RG70_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="1">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC124626040</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="1"/>
    <dbReference type="RefSeq" id="XP_053540030.1">
        <property type="nucleotide sequence ID" value="XM_053684055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RG70"/>
    <dbReference type="GeneID" id="124626040"/>
    <dbReference type="KEGG" id="ipu:124626040"/>
    <dbReference type="OrthoDB" id="26387at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044046">
        <property type="entry name" value="E3_ligase_UBR-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF24">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18995">
        <property type="entry name" value="PRT6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="E3 ubiquitin-protein ligase UBR-like C-terminal" evidence="2">
        <location>
            <begin position="17"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053540030.1"/>
        </source>
    </evidence>
    <sequence length="119" mass="13257" checksum="0AB2FCCB86A9FD4E" modified="2023-06-28" version="1">MSALTPIHQKLLCDSLRETSSGWHLWRCVKAGILPFLRGAALFFHHLTGVPIPPELKAVGAGELGSAVCICLPPNLLQLYYNHQELLDPLLQRWCSHPGMQQCLQSPETLKVIILCSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RHN6</accession>
    <name>A0A9F7RHN6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Translation initiation factor IF-2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC128635252</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053543085.1">
        <property type="nucleotide sequence ID" value="XM_053687110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RHN6"/>
    <dbReference type="GeneID" id="128635252"/>
    <dbReference type="KEGG" id="ipu:128635252"/>
    <dbReference type="OrthoDB" id="10628611at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053543085.1"/>
        </source>
    </evidence>
    <sequence length="360" mass="39377" checksum="73559AD26A291861" modified="2023-06-28" version="1">MDAAGERRRRRTRKSTPTIPAMDSVHFPQWTIMELPDPFDGFFGAPEYFLVDCQFYFSSLSDPKPEEKHWLRFMWSRFFGPARQWVQLKLDKHCRNLDSVEQFVGEFMMRFGSPEAKEELEQLLRGGSLEGEPALPFTHYYRQLHAESNSENQVKSQVPEIHVKPPVSIVQAKPPGPEAQAKPPGPEAQAKPPGPEAQAKPPGSEAQAKPPGSEAQAKPPGSDAQAKPPGSDAQAKPPGSDAQAKPPGSEAQAKPPGSDAQAKPHVPELTFKPADPVSQALLISQPNDQMLLTPKSTDPVTQVQPTDTHSQVPLTPRSADRPSRAPLTPRSADRPSRAPLTPRSANTPSPEFTWVCIAPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RL41</accession>
    <name>A0A9F7RL41_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">E3 ubiquitin-protein ligase TRIM39</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC108269710</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053538871.1">
        <property type="nucleotide sequence ID" value="XM_053682896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RL41"/>
    <dbReference type="GeneID" id="108269710"/>
    <dbReference type="KEGG" id="ipu:108269710"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13733">
        <property type="entry name" value="SPRY_PRY_C-I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
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        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
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        <property type="entry name" value="Znf-RING_euk"/>
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        <property type="entry name" value="Znf_B-box"/>
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        <property type="entry name" value="Znf_RING"/>
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        <property type="entry name" value="BLOODTHIRSTY-RELATED GENE FAMILY, MEMBER 16 ISOFORM X1-RELATED"/>
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    <dbReference type="Pfam" id="PF13445">
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    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
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    </dbReference>
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        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="56"/>
            <end position="96"/>
        </location>
    </feature>
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        <location>
            <begin position="178"/>
            <end position="218"/>
        </location>
    </feature>
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        <location>
            <begin position="377"/>
            <end position="574"/>
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    </feature>
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        <location>
            <begin position="1"/>
            <end position="36"/>
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        <location>
            <begin position="279"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_053538871.1"/>
        </source>
    </evidence>
    <sequence length="576" mass="66466" checksum="DD00BEE39317DF5A" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RLQ6</accession>
    <name>A0A9F7RLQ6_ICTPU</name>
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        <submittedName>
            <fullName evidence="6">C-type mannose receptor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC108261852</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053537822.1">
        <property type="nucleotide sequence ID" value="XM_053681847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RLQ6"/>
    <dbReference type="GeneID" id="108261852"/>
    <dbReference type="KEGG" id="ipu:108261852"/>
    <dbReference type="OrthoDB" id="6369810at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 20 MEMBER A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784:SF5">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 20 MEMBER A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039896381" evidence="3">
        <location>
            <begin position="24"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="21"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="143"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="457"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="579"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="311"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="329"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="421"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_053537822.1"/>
        </source>
    </evidence>
    <sequence length="702" mass="80503" checksum="7770F4F25A247680" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7RLT9</accession>
    <name>A0A9F7RLT9_ICTPU</name>
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        <submittedName>
            <fullName evidence="8">Centrosomal protein of 55 kDa</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC128633073</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053537698.1">
        <property type="nucleotide sequence ID" value="XM_053681723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7RLT9"/>
    <dbReference type="GeneID" id="128633073"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:midbody"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:establishment of protein localization"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:mitotic cytokinesis"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1180">
        <property type="entry name" value="Geminin coiled-coil domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.20.5.990">
        <property type="entry name" value="Nemo cc2-lz domain - 1d5 darpin complex"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CEP55"/>
    </dbReference>
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        <property type="entry name" value="EABR"/>
    </dbReference>
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        <property type="entry name" value="CENTROSOMAL PROTEIN OF 55 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31838:SF1">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 55 KDA"/>
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        <property type="entry name" value="EABR"/>
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        <location>
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        <location>
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        <location>
            <begin position="21"/>
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        </location>
    </feature>
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        <location>
            <begin position="131"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="143"/>
            <end position="156"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF12180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_053537698.1"/>
        </source>
    </evidence>
    <sequence length="340" mass="40067" checksum="CCAA2A6BB4488A3F" modified="2023-06-28" version="1">MGFKGAMKTFSKKLGFKSSGSKAVEAELEKLKKENAQLRMTLEDMSKQNGRLYPACPESDEAKLLEVPSDEITILKEQLRDAQEKNQKWKVYDHERESCMQWNLARRIELEQQLNQAQRTLEQQQEETKSEGQSPASMQQEKQLQESPREMEEERKRASRLQVELVALKAKYEKKSREVVRVQEELQVERRSWRQTLALRHEDQKRMAVLEQQIQSSARDFENEKQDRQSLQHLLHKVLKELRKARDQITRLDSAEIHVHQSITFKKKKLQRETRFSEPSSCTRLDLDKTTNQDRILNPTSPLKSSNTLDERFLDCPICSASYPVSQHRKLLEHIDYCFN</sequence>
</entry>
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    <accession>A0A9F7RM00</accession>
    <name>A0A9F7RM00_ICTPU</name>
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        <submittedName>
            <fullName evidence="6">Carcinoembryonic antigen-related cell adhesion molecule 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">si:cabz01036022.1</name>
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    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053537803.1">
        <property type="nucleotide sequence ID" value="XM_053681828.1"/>
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        <property type="component" value="Chromosome 7"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="leukocyte immune-type receptor 2 isoform X2"/>
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        <property type="entry name" value="Leukocyte immune-type receptor TS32.15 L2.5a"/>
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    </dbReference>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_Fc_receptor"/>
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        <property type="entry name" value="Ig_sub"/>
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        <property type="entry name" value="Ig_sub2"/>
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        <property type="entry name" value="FC RECEPTOR-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481">
        <property type="entry name" value="IMMUNOGLOBULIN FC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IG"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="6"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039918700" evidence="3">
        <location>
            <begin position="23"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="19"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="101"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="192"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="276"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="365"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="460"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_053537803.1"/>
        </source>
    </evidence>
    <sequence length="563" mass="63438" checksum="36B4954E873FA996" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TEC4</accession>
    <name>A0A9F7TEC4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Extracellular calcium-sensing receptor-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC124627838</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_053678324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TEC4"/>
    <dbReference type="GeneID" id="124627838"/>
    <dbReference type="KEGG" id="ipu:124627838"/>
    <dbReference type="OrthoDB" id="5984008at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF519">
        <property type="entry name" value="EXTRACELLULAR CALCIUM-SENSING RECEPTOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="263"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="300"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="333"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="377"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="422"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="457"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="489"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="13">
        <location>
            <begin position="263"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_053534299.1"/>
        </source>
    </evidence>
    <sequence length="527" mass="58260" checksum="928A9DF713478E7D" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TJ48</accession>
    <name>A0A9F7TJ48_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fumarylacetoacetate hydrolase domain-containing protein 2-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC128630370</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
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    <dbReference type="RefSeq" id="XP_053534811.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TJ48"/>
    <dbReference type="GeneID" id="128630370"/>
    <dbReference type="KEGG" id="ipu:128630370"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:small molecule metabolic process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
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        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
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        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE DOMAIN-CONTAINING PROTEIN 2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="3">
        <location>
            <begin position="63"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053534811.1"/>
        </source>
    </evidence>
    <sequence length="205" mass="23087" checksum="6C69E4CD21608540" modified="2023-06-28" version="1">MSADLWLLPMLHYCHSSSAKTNEANRRHETYPGYVWCEDKNHAKLEIHSYKKEASYAHACYSQEEDARGHVAGFTVASEISARVWQMKNGKHWLLGKTFDKFCPLEPALVTTEALKDVHNLGIRCLVNGDVVQDSNTSQLIFKTEKVVVWVSQFVTLYPGDVFLTGTPPGVGVFRKPPIFLKVSSVSSFLHCVGLFSIFQFCESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TJU6</accession>
    <name>A0A9F7TJU6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein LOC128632760</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC128632760</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SDA103</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053535937.1">
        <property type="nucleotide sequence ID" value="XM_053679962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TJU6"/>
    <dbReference type="GeneID" id="128632760"/>
    <dbReference type="KEGG" id="ipu:128632760"/>
    <dbReference type="OrthoDB" id="8941791at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_053535937.1"/>
        </source>
    </evidence>
    <sequence length="402" mass="44705" checksum="0ED1ED23353591B9" modified="2023-06-28" version="1">MDYYFQDGTTKTPAAFLNELKPVLGNVLIRIRLVFKNLTRVPSEADVLNAANALLDSKIRRRRSLVIQTLSDPVSIQNVIYEKIGNNSYIISFAFQITNVTISKDLQLRNGTYDLIQNTINSLLNTILNVKDAPAFVFPQANYTFNGTAIEANFEYIFVEGETKWTPSGFLSANLNISELSTFTTPAAQTESTVHVVLRPTTLVVVQTRMLFNSSCPVPSESLVLRAIQTLLSAQLTNLTDSVNRLNFTYEKISETSYAVFFTLSISNISMSKNAELRNDTYTQVENIINNALNTLLNDAGAAPFEPQSSFFTSSGNQVNGDMEYYFQDGDTKTPAAFLDELTPVLGNVLIRIRLVFKNLTRVPSEADVLNAANALLDSKIRRAQCLITANQQLHFQECTAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TJZ0</accession>
    <name>A0A9F7TJZ0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Glucocorticoid modulatory element-binding protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">gmeb1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_053539984.1">
        <property type="nucleotide sequence ID" value="XM_053684009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TJZ0"/>
    <dbReference type="GeneID" id="108273313"/>
    <dbReference type="CTD" id="10691"/>
    <dbReference type="OrthoDB" id="5792412at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.390.10:FF:000003">
        <property type="entry name" value="glucocorticoid modulatory element-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.390.10">
        <property type="entry name" value="SAND domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059099">
        <property type="entry name" value="GMEB1/2/Spe-44_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010919">
        <property type="entry name" value="SAND-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000770">
        <property type="entry name" value="SAND_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10417">
        <property type="entry name" value="GLUCOCORTICOID MODULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10417:SF3">
        <property type="entry name" value="GLUCOCORTICOID MODULATORY ELEMENT-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01342">
        <property type="entry name" value="SAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25892">
        <property type="entry name" value="Spe-44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00258">
        <property type="entry name" value="SAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63763">
        <property type="entry name" value="SAND domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50864">
        <property type="entry name" value="SAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SAND" evidence="11">
        <location>
            <begin position="121"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="389"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="537"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="389"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="541"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_053539984.1"/>
        </source>
    </evidence>
    <sequence length="605" mass="66043" checksum="830C7331A29108AB" modified="2023-06-28" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TLK1</accession>
    <name>A0A9F7TLK1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein LOC108267154</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC108267154</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053537842.1">
        <property type="nucleotide sequence ID" value="XM_053681867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TLK1"/>
    <dbReference type="GeneID" id="108267154"/>
    <dbReference type="KEGG" id="ipu:108267154"/>
    <dbReference type="OrthoDB" id="9447832at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00169">
        <property type="entry name" value="Chemokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00272">
        <property type="entry name" value="Chemokine_CC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039809">
        <property type="entry name" value="Chemokine_b/g/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015">
        <property type="entry name" value="SMALL INDUCIBLE CYTOKINE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00199">
        <property type="entry name" value="SCY"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0202">Cytokine</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="14"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="4">
        <location>
            <begin position="50"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="4">
        <location>
            <begin position="125"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="4">
        <location>
            <begin position="200"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="274"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="277"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_053537842.1"/>
        </source>
    </evidence>
    <sequence length="334" mass="37400" checksum="74FF63C70EAF19E7" modified="2023-06-28" version="1">MTVFISSPSSSSSSSFICIIIIIMFCRSLLLVLLVLVCLQSITTFLNFPSLECCLDYQTNPIPVIRIIAYEIFDLFCIEPVIFFFLYDGPPVCANPKDEWVQKLMKTIDSHSFTSWVEFEDAIRPMECCSSYITYPLPLRVFTAYKVIDHQCIKPGVIFTVHHGPRMCANPTDEWVQMVIDVIDSRPFTTLVETEAGLGQMECCNIYINHTLNERDVTTYEVTDHQCIKPGVIFTANRGPRVCANPTDEWVQELMNTIDSLLFTSLVKPKVFQPEPVSTPEPVSTPEPVSTPEPVSTPKPVSTPEPVSTASPDIESTSPPAQQDYTEESVYGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TMG1</accession>
    <name>A0A9F7TMG1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine/arginine repetitive matrix protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">srrm1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the splicing factor SR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_053538727.1">
        <property type="nucleotide sequence ID" value="XM_053682752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TMG1"/>
    <dbReference type="GeneID" id="108270153"/>
    <dbReference type="CTD" id="10250"/>
    <dbReference type="OrthoDB" id="163257at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048024">
        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1390.10:FF:000002">
        <property type="entry name" value="Serine/arginine repetitive matrix 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1390.10">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002483">
        <property type="entry name" value="PWI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036483">
        <property type="entry name" value="PWI_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052225">
        <property type="entry name" value="Ser/Arg_repetitive_matrix"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23148">
        <property type="entry name" value="SERINE/ARGININE REGULATED NUCLEAR MATRIX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23148:SF0">
        <property type="entry name" value="SERINE_ARGININE REPETITIVE MATRIX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01480">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00311">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101233">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51025">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWI" evidence="6">
        <location>
            <begin position="27"/>
            <end position="126"/>
        </location>
    </feature>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
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                <person name="Kucuktas H."/>
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                <person name="Liu H."/>
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                <person name="Xie Y."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
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                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
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    <dbReference type="KEGG" id="ipu:128634973"/>
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    <dbReference type="Proteomes" id="UP000221080">
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    </dbReference>
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            <begin position="143"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_053541936.1"/>
        </source>
    </evidence>
    <sequence length="284" mass="31503" checksum="D5D4AD28B48B06FD" modified="2023-06-28" version="1">MVRLELEDVEKQIRGLLDKQAQLLERQTALETSCASAHISKVSTQRGISTPSPSTPCVSLCRDRAPRTFPAVVSVTPAPTHLGPWVNQRRKARAGPSPPPVFEIPTRNRFAPLRQTRPNTVIIGDSIVRNVRVASSKGKVRTHCFSGACVLDVAAQVSGILKKDERIGAVVLHAGTNDTRLRQTEVLKRDFSSLIETVRGRSPTAKIIVSGPLPTYRRGAEKFSRLLALNDWLVSWCNEQNLVFVNNWNLFWERPRLFRPDGLHPSSLGAELLSDNISKALHSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-06-28" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9F7TR99</accession>
    <name>A0A9F7TR99_ICTPU</name>
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        <submittedName>
            <fullName evidence="5">Solute carrier family 35 member F4</fullName>
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        <name evidence="5" type="primary">LOC108276659</name>
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    <organism evidence="4 5">
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_053543032.1">
        <property type="nucleotide sequence ID" value="XM_053687057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9F7TR99"/>
    <dbReference type="GeneID" id="108276659"/>
    <dbReference type="KEGG" id="ipu:108276659"/>
    <dbReference type="OrthoDB" id="10062838at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026505">
        <property type="entry name" value="Solute_c_fam_35_mem_F3/F4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19346:SF2">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER F4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19346">
        <property type="entry name" value="SUGAR PHOSPHATE TRANSPORTER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="226"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="255"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="318"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="347"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="59"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053543032.1"/>
        </source>
    </evidence>
    <sequence length="389" mass="42601" checksum="E731EB79B58FCC86" modified="2023-06-28" version="1">MNKLSAKVSPCSTLPPATPHTASTSEADVRSEESVSVQESGPSERPCRCPVWAMIRLAAGVVLGVGMATTWVGAAHSAKQTLTHFNAPFFIFWFCSIWNLLMFPLYYIGYLLTEKHRETPTARFRKCLQFLGDGDVTVRVLLRFSAPFSMFWSVSGFLYLRALSRTSVTDCSAVMCCNSAFTFLLTWICLKERFLGVRVVAVILSITGIVMLAYSDGFYSDSITGVALGVGSASCSALYNVLYRKRVGTLDPGPASVLLCCVGICVFVLHSWVCVLLYVTHMEFWPPSQPVPWNTLCTTASLLLVFNVLVYMGGVCTYPALISLGVLLTVPASAAVDVWVLEAPPLSDMRSGALGLISAAFVLLLFPEDWDEKTVQWISSVWTQKTLNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3TE95</accession>
    <name>E3TE95_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">RASH</name>
        <name evidence="14" type="synonym">hras</name>
        <name evidence="14" type="synonym">hrasa</name>
        <name evidence="14" type="synonym">rash</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="EMBL" id="GU588675">
        <property type="protein sequence ID" value="ADO28631.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187429.1">
        <property type="nucleotide sequence ID" value="NM_001200500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TE95"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008931"/>
    <dbReference type="GeneID" id="100528230"/>
    <dbReference type="KEGG" id="ipu:100528230"/>
    <dbReference type="CTD" id="550286"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04138">
        <property type="entry name" value="H_N_K_Ras_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000096">
        <property type="entry name" value="KRAS proto-oncogene, GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADO28631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="NP_001187429.1"/>
        </source>
    </evidence>
    <sequence length="189" mass="21422" checksum="10D4F13B80702B76" modified="2011-01-11" version="1">MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDLPARTADTRQAHELARSYGIPYIETSAKTRQGVEDAFYTLVREIRQHKLRKLNPPDESGQDCMSCRCVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3TFH8</accession>
    <name>E3TFH8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Complex I-20kD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">NADH-ubiquinone oxidoreductase 20 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">NDUS7</name>
        <name evidence="14" type="synonym">ndufs7</name>
        <name evidence="14" type="synonym">ndus7</name>
    </gene>
    <organism evidence="12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E11546" last="E11546">
            <title>Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).</title>
            <authorList>
                <person name="Chen F."/>
                <person name="Lee Y."/>
                <person name="Jiang Y."/>
                <person name="Wang S."/>
                <person name="Peatman E."/>
                <person name="Abernathy J."/>
                <person name="Liu H."/>
                <person name="Liu S."/>
                <person name="Kucuktas H."/>
                <person name="Ke C."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="20634964"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0011546"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity of complex I.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Core subunit of respiratory chain NADH dehydrogenase (Complex I) which is composed of 45 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the complex I 20 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="GU589112">
        <property type="protein sequence ID" value="ADO29064.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187743.1">
        <property type="nucleotide sequence ID" value="NM_001200814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3TFH8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009978"/>
    <dbReference type="GeneID" id="100528544"/>
    <dbReference type="KEGG" id="ipu:100528544"/>
    <dbReference type="CTD" id="374291"/>
    <dbReference type="OrthoDB" id="268400at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12280:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit B 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01356">
        <property type="entry name" value="NDH1_NuoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006138">
        <property type="entry name" value="NADH_UQ_OxRdtase_20Kd_su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01957">
        <property type="entry name" value="nuoB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005012">
        <property type="entry name" value="PRK06411.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11995">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11995:SF14">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56770">
        <property type="entry name" value="HydA/Nqo6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01150">
        <property type="entry name" value="COMPLEX1_20K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 10" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase-like 20kDa subunit" evidence="11">
        <location>
            <begin position="92"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADO29064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="NP_001187743.1"/>
        </source>
    </evidence>
    <sequence length="217" mass="24222" checksum="45FEC3B433EC8DF1" modified="2011-01-11" version="1">MAALVAPRLATLCSFSLRPFSSFAAQKTVLLKNTKDAEASSLVPFREKSTAVALKPTSAVSSKGEYVITKLDDLVNWARRSSLWPMTFGLACCAVEMMHMAAPRYDMDRFGVVFRASPRQADVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGTLQLQRKIKREKKMRIWYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-03-01" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>Q8UWL2</accession>
    <name>Q8UWL2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11 13">Novel immune-type receptor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">NITR3</name>
        <name evidence="13" type="synonym">NITR1</name>
        <name evidence="13" type="synonym">nitr3</name>
    </gene>
    <organism evidence="11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13" key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="13832" last="13837">
            <title>Extraordinary variation in a diversified family of immune-type receptor genes.</title>
            <authorList>
                <person name="Hawke N.A."/>
                <person name="Yoder J.A."/>
                <person name="Haire R.N."/>
                <person name="Mueller M.G."/>
                <person name="Litman R.T."/>
                <person name="Miracle A.L."/>
                <person name="Stuge T."/>
                <person name="Shen L."/>
                <person name="Miller N."/>
                <person name="Litman G.W."/>
            </authorList>
            <dbReference type="PubMed" id="11698645"/>
            <dbReference type="DOI" id="10.1073/pnas.231418598"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AF397456">
        <property type="protein sequence ID" value="AAL35544.2"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="NP_001187024.1">
        <property type="nucleotide sequence ID" value="NM_001200095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8UWL2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000015192"/>
    <dbReference type="GeneID" id="100304509"/>
    <dbReference type="KEGG" id="ipu:100304509"/>
    <dbReference type="CTD" id="100304509"/>
    <dbReference type="OrthoDB" id="6370831at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF133">
        <property type="entry name" value="IMMUNE-TYPE RECEPTOR 5 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9 13" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5011323840" evidence="9 13">
        <location>
            <begin position="19"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="249"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="27"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="117"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAL35544.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="NP_001187024.1"/>
        </source>
    </evidence>
    <sequence length="280" mass="31247" checksum="2CB249026222BEF7" modified="2002-03-01" version="1">MITLFVALSLVITVKTSGVKDLQVQKVKRGGDVTIKCENLYTEVNFIWFKQSFGKFPQEVVKQVDKRNRYGAQFNDGRFSISEDKKPFNLNIKEIKEEDSGTYFCAELQASSLRFAPGTLLVVEAEEMKHHPPTVTVMENGESLTLQCSVQVFTSSCEGQSVYWFRHGSGESHPGIIYTHGDGSDECKKSSEAGSPTQSCVYTLPKRNLTTSDNGTFYCAVAACGQILFGNGIKVEVKGSSDNTDQMDLLFWLSVFRVGVLAFILIIFTMYYNVLKLRSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>W5U6H8</accession>
    <name>W5U6H8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GTP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RRAD</name>
        <name evidence="8" type="synonym">rrad</name>
    </gene>
    <organism evidence="6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="6">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Interacts with Calmodulin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the small GTPase superfamily. RGK family.</text>
    </comment>
    <dbReference type="EMBL" id="JT405406">
        <property type="protein sequence ID" value="AHH37174.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017313815.1">
        <property type="nucleotide sequence ID" value="XM_017458326.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U6H8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034965"/>
    <dbReference type="GeneID" id="108259109"/>
    <dbReference type="KEGG" id="ipu:108259109"/>
    <dbReference type="CTD" id="6236"/>
    <dbReference type="GeneTree" id="ENSGT00940000159836"/>
    <dbReference type="OMA" id="LPSHCMK"/>
    <dbReference type="OrthoDB" id="5239715at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005246">
        <property type="term" value="F:calcium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04148">
        <property type="entry name" value="RGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000311">
        <property type="entry name" value="GTP-binding protein RAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017358">
        <property type="entry name" value="RGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051641">
        <property type="entry name" value="RGK_GTP-binding_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45775:SF3">
        <property type="entry name" value="GTP-BINDING PROTEIN RAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45775">
        <property type="entry name" value="RAD, GEM/KIR FAMILY MEMBER 2, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038017">
        <property type="entry name" value="GTP-binding_GEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR038017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AHH37174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017313815.1"/>
        </source>
    </evidence>
    <sequence length="307" mass="34940" checksum="D6AA2823F62B2575" modified="2014-04-16" version="1">MTLNKGDKLRNMDKRRGSTPFPMHLQTLHRRSMPVDDRELRSSMPPVLAGELTNLTRCTSYSPGEQHRDSCVSDSSDSVISSGSDSDGQIYKVVLLGEHGVGKSTLARIFGGVEDTHDYEDAGNTYDRSIIVDEEEANILLYDVWEQDNSQWLKDQCMRMGDAYIIVYSVTDKSSFEKASELRIQLRRARQSENIPIILVGNKTDLVRSREVSVDEGSACAVVFDCKFIETSASLHHNVRTLFEGIVRQIRLRRDSKEENARRMANCKRRESISKKAKRFLGRMVARKNKKMAFRQKSKSCHDLSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5U914</accession>
    <name>W5U914_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="15 17">Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">MTHFD2</name>
        <name evidence="17" type="synonym">mthfd2</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(6R)-5,10-methenyltetrahydrofolate + H2O = (6R)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-methenyltetrahydrofolate + NADH</text>
            <dbReference type="Rhea" id="RHEA:22892"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.5.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="JT407655">
        <property type="protein sequence ID" value="AHH38064.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017307544.1">
        <property type="nucleotide sequence ID" value="XM_017452055.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5U914"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030788"/>
    <dbReference type="GeneID" id="108255807"/>
    <dbReference type="KEGG" id="ipu:108255807"/>
    <dbReference type="CTD" id="10797"/>
    <dbReference type="GeneTree" id="ENSGT00940000164825"/>
    <dbReference type="OMA" id="VCHILTK"/>
    <dbReference type="OrthoDB" id="5126881at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004487">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000001">
        <property type="entry name" value="Bifunctional protein FolD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000070">
        <property type="entry name" value="probable bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020867">
        <property type="entry name" value="THF_DH/CycHdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF15">
        <property type="entry name" value="BIFUNCTIONAL METHYLENETETRAHYDROFOLATE DEHYDROGENASE_CYCLOHYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00766">
        <property type="entry name" value="THF_DHG_CYH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00767">
        <property type="entry name" value="THF_DHG_CYH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="13">
        <location>
            <begin position="33"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding" evidence="14">
        <location>
            <begin position="167"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AHH38064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017307544.1"/>
        </source>
    </evidence>
    <sequence length="338" mass="36938" checksum="6FCDB095BFC73FFA" modified="2014-04-16" version="1">MAGVRTLRKVCQRAQHHARDLHLSVSRTEAVVISGRKLARQIRDEARVDVEKWVTTGNRRPHLSVVLVGDNPASHSYVLNKTRAAADVGITSETILKPSSVSEEELMELIDKLNSDHRVDGLLVQLPLPEHIDERRICNAVSPDKDVDGFHVVNVGRLCLDQSSMLPATPWGVWEIIKRTGIPTLGKNVLVAGRSKNVGMPIAMLLHTDGRHERPGGDATVTISHRYTPKEQLRQHTKIADIIVAAAGIPNLITADMIKEGAAVIDVGINRIQDPLTGKDRLVGDVDFEGVRKKASYITPVPGGVGPMTVAMLMKNTIKAAKNLLLTPPERMRMVASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5UAY3</accession>
    <name>W5UAY3_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Phosphatidylserine synthase</fullName>
            <ecNumber evidence="15">2.7.8.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Serine-exchange enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ptdss1</name>
        <name evidence="19" type="synonym">ptdss1b</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes a base-exchange reaction in which the polar head group of phosphatidylethanolamine (PE) is replaced by L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + L-serine = a 1,2-diacyl-sn-glycero-3-phospho-L-serine + ethanolamine</text>
            <dbReference type="Rhea" id="RHEA:27606"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="EC" id="2.7.8.29"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:27607"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Phospholipid metabolism; phosphatidylserine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Endoplasmic reticulum membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the phosphatidyl serine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.29" evidence="15"/>
    <dbReference type="EMBL" id="JT410792">
        <property type="protein sequence ID" value="AHH39191.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017310625.1">
        <property type="nucleotide sequence ID" value="XM_017455136.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UAY3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021666"/>
    <dbReference type="GeneID" id="108257397"/>
    <dbReference type="KEGG" id="ipu:108257397"/>
    <dbReference type="CTD" id="559442"/>
    <dbReference type="OMA" id="LGQNAWM"/>
    <dbReference type="OrthoDB" id="10265393at2759"/>
    <dbReference type="UniPathway" id="UPA00948"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106245">
        <property type="term" value="F:L-serine-phosphatidylethanolamine phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006659">
        <property type="term" value="P:phosphatidylserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004277">
        <property type="entry name" value="PSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362:SF33">
        <property type="entry name" value="PHOSPHATIDYLSERINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03034">
        <property type="entry name" value="PSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 15" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10 15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 15" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="13 15" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="354"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="431"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="460"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AHH39191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017310625.1"/>
        </source>
    </evidence>
    <sequence length="469" mass="54659" checksum="5D5949FD979C49F4" modified="2014-04-16" version="1">MASSPDSRTLSKDDVHYKFHFRMINEQQVEDITIEFFYKPHTITLLTFTIISIMYFAFTRNDSDPDNNLRVGLALVVSFFLVISVLAFPNGPFTRPHPAIWRMVFGLSVLYFLFLVFLIFLNWDQVKLLMYWLDPNLRYATREADVMEYAVNCHMITWERILSHFDIFAFGHFWGWAMKALLIRSYGLCWTISITWELTELFFMHLLPNFAECWWDQVILDILLCNGGGIWLGMTVCHFLEMRTYRWASIKDIHSATGKLKRAVLQFTPASWTYVRWFDPKSSFQRVAGIYLFMIIWQLTELNTFFLKHIFVFQASHPLSWCRILFVGIITAPTVRQYYAYLTDTQCKRVGTQCWVFGAIAFLEALACVKFGQDLFSKTQVMYVILWLVCVAFITSLCLYGMVWHEQKYGGRLKLQSTSDCDDSSFIDSSGYTPDTIKGERDSSNSQLLHQRKGGPVKNKATNGTNNKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5UDL6</accession>
    <name>W5UDL6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">15-hydroxyprostaglandin dehydrogenase [NAD(+)]</fullName>
            <ecNumber evidence="4">1.1.1.141</ecNumber>
            <ecNumber evidence="5">1.1.1.232</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Eicosanoid/docosanoid dehydrogenase [NAD(+)]</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Prostaglandin dehydrogenase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">HPGD</name>
        <name evidence="26" type="synonym">hpgd</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="24">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">SDA103</strain>
        </source>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the NAD-dependent dehydrogenation (oxidation) of a broad array of hydroxylated polyunsaturated fatty acids (mainly eicosanoids and docosanoids, including prostaglandins, lipoxins and resolvins), yielding their corresponding keto (oxo) metabolites. Decreases the levels of the pro-proliferative prostaglandins such as prostaglandin E2 (whose activity is increased in cancer because of an increase in the expression of cyclooxygenase 2) and generates oxo-fatty acid products that can profoundly influence cell function by abrogating pro-inflammatory cytokine expression. Converts resolvins E1, D1 and D2 to their oxo products, which represents a mode of resolvin inactivation. Resolvin E1 plays important roles during the resolution phase of acute inflammation, while resolvins D1 and D2 have a unique role in obesity-induced adipose inflammation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(11R)-hydroxy-(5Z,8Z,12E,14Z)-eicosatetraenoate + NAD(+) = 11-oxo-(5Z,8Z,12E,14Z)-eicosatetraenoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48640"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78836"/>
            <dbReference type="ChEBI" id="CHEBI:90697"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:48641"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>(15S)-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + NAD(+) = 15-oxo-(5Z,8Z,11Z,13E)-eicosatetraenoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23260"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57409"/>
            <dbReference type="ChEBI" id="CHEBI:57410"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.232"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:23261"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>14-hydroxy-(4Z,7Z,10Z,12E,16Z,19Z)-docosahexaenoate + NAD(+) = 14-oxo-(4Z,7Z,10Z,12E,16Z,19Z)-docosahexaenoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48952"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:90866"/>
            <dbReference type="ChEBI" id="CHEBI:90867"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:48953"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>15-oxo-(5S,6R)-dihydroxy-(7E,9E,11Z)-eicosatrienoate + NADH + H(+) = (5S,6R,15S)-trihydroxy-(7E,9E,11Z)-eicosatrienoate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:41596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78325"/>
            <dbReference type="ChEBI" id="CHEBI:78329"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:41597"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>lipoxin A4 + NAD(+) = 15-oxo-(5S,6R)-dihydroxy-(7E,9E,11Z,13E)-eicosatetraenoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41572"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:67026"/>
            <dbReference type="ChEBI" id="CHEBI:78311"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:41573"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>prostaglandin A1 + NAD(+) = 15-oxo-prostaglandin A1 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41263"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57398"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:85072"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:41264"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>prostaglandin E1 + NAD(+) = 15-oxoprostaglandin E1 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16477"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57397"/>
            <dbReference type="ChEBI" id="CHEBI:57401"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:16478"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>prostaglandin E2 + NAD(+) = 15-oxoprostaglandin E2 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11876"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57400"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:606564"/>
            <dbReference type="EC" id="1.1.1.141"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:11877"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>resolvin D1 + NAD(+) = 17-oxoresolvin D1 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50128"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132079"/>
            <dbReference type="ChEBI" id="CHEBI:132081"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:50129"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>resolvin D1 + NAD(+) = 8-oxoresolvin D1 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50124"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132079"/>
            <dbReference type="ChEBI" id="CHEBI:132080"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:50125"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>resolvin D2 + NAD(+) = 16-oxoresolvin D2 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53588"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:133367"/>
            <dbReference type="ChEBI" id="CHEBI:137498"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:53589"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>resolvin D2 + NAD(+) = 7-oxoresolvin D2 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:133367"/>
            <dbReference type="ChEBI" id="CHEBI:137497"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:53585"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>resolvin E1 + NAD(+) = 18-oxo-resolvin E1 + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49244"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:91000"/>
            <dbReference type="ChEBI" id="CHEBI:91001"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:49245"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 23">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.141" evidence="4"/>
    <dbReference type="EC" id="1.1.1.232" evidence="5"/>
    <dbReference type="EMBL" id="JT412942">
        <property type="protein sequence ID" value="AHH40058.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017318874.1">
        <property type="nucleotide sequence ID" value="XM_017463385.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UDL6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000004392"/>
    <dbReference type="GeneID" id="108263011"/>
    <dbReference type="KEGG" id="ipu:108263011"/>
    <dbReference type="CTD" id="3248"/>
    <dbReference type="GeneTree" id="ENSGT00940000154593"/>
    <dbReference type="OMA" id="RSMTKFG"/>
    <dbReference type="OrthoDB" id="37659at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047034">
        <property type="term" value="F:15-hydroxyicosatetraenoate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016404">
        <property type="term" value="F:15-hydroxyprostaglandin dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006693">
        <property type="term" value="P:prostaglandin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05323">
        <property type="entry name" value="ADH_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000149">
        <property type="entry name" value="15-hydroxyprostaglandin dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44229:SF2">
        <property type="entry name" value="15-HYDROXYPROSTAGLANDIN DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44229">
        <property type="entry name" value="15-HYDROXYPROSTAGLANDIN DEHYDROGENASE [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="2" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0644">Prostaglandin metabolism</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00049188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="AHH40058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_017318874.1"/>
        </source>
    </evidence>
    <sequence length="261" mass="28634" checksum="894D87504680EC2E" modified="2014-04-16" version="1">MALRGRVALVTGGVQGIGRAAAESLLEKHAKVALVDLNQSVGEECKKELDEQYGQDNSIFLQCDVTDGGKLQEAFKSTVEHFGRLDIVINNAGINNEKNWEKTIEVNLTSVIKGTYLALEHMSKEYGKEGGVIINVSSMAAFLHSPYQPVYTATKHGVIGFSRALADASEHCNYGVRINVLCPAFVDTQLLLSVDCEETMGKFIKYKDDLKQKMDKYGVLKPSLIAEGMMRLVTDESLNGAVMKITCSKGIHFHVYEPLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5UDV7</accession>
    <name>W5UDV7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">DEAH-box protein 16</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">DHX16</name>
        <name evidence="27" type="synonym">dhx16</name>
    </gene>
    <organism evidence="25">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="25">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">SDA103</strain>
        </source>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="27">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Required for pre-mRNA splicing as a component of the spliceosome. Contributes to pre-mRNA splicing after spliceosome formation and prior to the first transesterification reaction. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs. Also plays a role in innate antiviral response by acting as a pattern recognition receptor sensing splicing signals in viral RNA. Mechanistically, TRIM6 promotes the interaction between unanchored 'Lys-48'-polyubiquitin chains and DHX16, leading to DHX16 interaction with RIGI and ssRNA to amplify RIGI-dependent innate antiviral immune responses.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Component of pre-catalytic spliceosome complexes. Component of the minor spliceosome, which splices U12-type introns. Interacts with GPKOW. Interacts with TRIM6. Interacts with RIGI.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the DEAD box helicase family. DEAH subfamily. DDX16/PRP8 sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="JT413192">
        <property type="protein sequence ID" value="AHH40148.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017346468.1">
        <property type="nucleotide sequence ID" value="XM_017490979.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UDV7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000001443"/>
    <dbReference type="GeneID" id="108277923"/>
    <dbReference type="KEGG" id="ipu:108277923"/>
    <dbReference type="CTD" id="8449"/>
    <dbReference type="OMA" id="PLDPMMS"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17974">
        <property type="entry name" value="DEXHc_DHX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000001">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000007">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000818">
        <property type="entry name" value="pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF83">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ATP-DEPENDENT RNA HELICASE DHX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 25 27" id="KW-0347">Helicase</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0391">Immunity</keyword>
    <keyword evidence="6" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="14" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="23">
        <location>
            <begin position="422"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="24">
        <location>
            <begin position="611"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="96"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1032"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="105"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="131"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="168"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00060352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00061692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00062452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00069571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00079549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="AHH40148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_017346468.1"/>
        </source>
    </evidence>
    <sequence length="1054" mass="121452" checksum="9C284CB6C28FA2F2" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5UDZ8</accession>
    <name>W5UDZ8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein lin-7 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LIN7C</name>
        <name evidence="15" type="synonym">lin7c</name>
    </gene>
    <organism evidence="13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="13">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the lin-7 family.</text>
    </comment>
    <dbReference type="EMBL" id="JT407235">
        <property type="protein sequence ID" value="AHH37917.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017314754.1">
        <property type="nucleotide sequence ID" value="XM_017459265.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UDZ8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026713"/>
    <dbReference type="GeneID" id="108259625"/>
    <dbReference type="KEGG" id="ipu:108259625"/>
    <dbReference type="CTD" id="55327"/>
    <dbReference type="GeneTree" id="ENSGT00940000153222"/>
    <dbReference type="OrthoDB" id="10056216at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097025">
        <property type="term" value="C:MPP7-DLG1-LIN7 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007269">
        <property type="term" value="P:neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06796">
        <property type="entry name" value="PDZ_Lin-7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000076">
        <property type="entry name" value="Protein lin-7 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.650">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014775">
        <property type="entry name" value="L27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036892">
        <property type="entry name" value="L27_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017365">
        <property type="entry name" value="LIN7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051109">
        <property type="entry name" value="MAM_complex_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14063">
        <property type="entry name" value="PROTEIN LIN-7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02828">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038039">
        <property type="entry name" value="Lin-7_homologue"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00569">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101288">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51022">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="8 10" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="L27" evidence="12">
        <location>
            <begin position="10"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="11">
        <location>
            <begin position="93"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR038039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AHH37917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017314754.1"/>
        </source>
    </evidence>
    <sequence length="201" mass="22294" checksum="723475E03AAE81A0" modified="2014-04-16" version="1">MASLGEPVRLERDISRAIELLDKLQKTGEVPPQKLQALQRVLQSEFCNAVREVYEHVYETVDINSSPEVRANATAKATVAAFAASEGHSHPRVVELPKTEEGLGFNIMGGKEQNSPIYISRIIPGGIADRHGGLKRGDQLLSVNGVSVEGEHHEKAVELLKAAQGTVKLVVRYTPKVLEEMESRFEKMRSAKRRQQNSYPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>W5UEC6</accession>
    <name>W5UEC6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8 10">Pleckstrin homology domain-containing family M member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PLEKHM3</name>
        <name evidence="10" type="synonym">plekhm3</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="8">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT407662">
        <property type="protein sequence ID" value="AHH38067.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017326084.1">
        <property type="nucleotide sequence ID" value="XM_017470595.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UEC6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035387"/>
    <dbReference type="GeneID" id="108266826"/>
    <dbReference type="KEGG" id="ipu:108266826"/>
    <dbReference type="CTD" id="389072"/>
    <dbReference type="GeneTree" id="ENSGT00940000159743"/>
    <dbReference type="OMA" id="CVHRELH"/>
    <dbReference type="OrthoDB" id="62364at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00029">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051366">
        <property type="entry name" value="DEF8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025258">
        <property type="entry name" value="RH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12326">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12326:SF10">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY M MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13901">
        <property type="entry name" value="RH_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01175">
        <property type="entry name" value="DUF4206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="305"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="7">
        <location>
            <begin position="621"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="94"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="419"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="425"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AHH38067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017326084.1"/>
        </source>
    </evidence>
    <sequence length="708" mass="79776" checksum="BC25BC36719DAEF1" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>W5UEC9</accession>
    <name>W5UEC9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GTPase Era, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">ERA-like protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19 21" type="primary">eral1</name>
    </gene>
    <organism evidence="19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="19">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Probable GTPase that plays a role in the mitochondrial ribosomal small subunit assembly. Specifically binds the 12S mitochondrial rRNA (12S mt-rRNA) to a 33 nucleotide section delineating the 3' terminal stem-loop region. May act as a chaperone that protects the 12S mt-rRNA on the 28S mitoribosomal subunit during ribosomal small subunit assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Era GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="JT413541">
        <property type="protein sequence ID" value="AHH40293.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017316177.1">
        <property type="nucleotide sequence ID" value="XM_017460688.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UEC9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019791"/>
    <dbReference type="GeneID" id="108260407"/>
    <dbReference type="KEGG" id="ipu:108260407"/>
    <dbReference type="CTD" id="26284"/>
    <dbReference type="OMA" id="WAEVDVI"/>
    <dbReference type="OrthoDB" id="8954335at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043024">
        <property type="term" value="F:ribosomal small subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04163">
        <property type="entry name" value="Era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22534">
        <property type="entry name" value="KH-II_Era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.20:FF:000016">
        <property type="entry name" value="GTPase Era, mitochondrial isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00367">
        <property type="entry name" value="GTPase_Era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030388">
        <property type="entry name" value="G_ERA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005662">
        <property type="entry name" value="GTPase_Era-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42698">
        <property type="entry name" value="GTPASE ERA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42698:SF1">
        <property type="entry name" value="GTPASE ERA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51713">
        <property type="entry name" value="G_ERA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="12 16" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Era-type G" evidence="18">
        <location>
            <begin position="104"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="16">
        <location>
            <begin position="112"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="16">
        <location>
            <begin position="138"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="16">
        <location>
            <begin position="159"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="16">
        <location>
            <begin position="228"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="265"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="16">
        <location>
            <begin position="321"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="265"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AHH40293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_017316177.1"/>
        </source>
    </evidence>
    <sequence length="450" mass="50253" checksum="A9DF7E57A651782F" modified="2014-04-16" version="1">MALRMCESLLCRTLRLSSLAASMETAPLRLRTAHHGCAVRNAAFRFVPTCFISTEAFLERLLQERTLGFAENPAGSPAHAAVPLDRVEHFSLMLKDPDQPENPKVLRVAIIGAPNAGKSTLTNQLLGKKLFATSQKVHTTRSRALGVITEDNTQIILLDTPGLTTPSKAKRHQLEKTFLVDPLKSLRQADLVVVLVDVSDKWTRSRLDFEVLKGLAMNAHIPAVLVLNKVDLLKSKPLLLDITEELTEGIVNGKRLKVRSLVKPLQKETQEKQTEKTAGLQNGEEHMPNPENQLLKGPDREQLRALKSCKGWPHFKDVFMMCATDGEDVQTLKSYLMVEAKPGPWQYHSAVLTDQSPEDICTNTIREKLLEYLPQEVPYTVTQYIELWREAADGNLDISVKLHVKKDSHMKMVIGPGGQLIARIAREAALDLMNAFMCEVRLKISATLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>W5UF57</accession>
    <name>W5UF57_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Secretory carrier-associated membrane protein</fullName>
            <shortName evidence="17">Secretory carrier membrane protein</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">scamp5</name>
        <name evidence="20 21 22" type="synonym">scamp5a</name>
    </gene>
    <organism evidence="18">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="18">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20 21 22" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20 21 22">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Secretory vesicle</location>
            <location evidence="3">Synaptic vesicle membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the SCAMP family. SCAMP5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="JT413308">
        <property type="protein sequence ID" value="AHH40199.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017341038.1">
        <property type="nucleotide sequence ID" value="XM_017485549.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053541526.1">
        <property type="nucleotide sequence ID" value="XM_053685551.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053541527.1">
        <property type="nucleotide sequence ID" value="XM_053685552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UF57"/>
    <dbReference type="GeneID" id="108275016"/>
    <dbReference type="KEGG" id="ipu:108275016"/>
    <dbReference type="CTD" id="324467"/>
    <dbReference type="GeneTree" id="ENSGT00940000157577"/>
    <dbReference type="OMA" id="VQFFVFY"/>
    <dbReference type="OrthoDB" id="242866at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007273">
        <property type="entry name" value="SCAMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10687:SF5">
        <property type="entry name" value="SECRETORY CARRIER-ASSOCIATED MEMBRANE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10687">
        <property type="entry name" value="SECRETORY CARRIER-ASSOCIATED MEMBRANE PROTEIN SCAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04144">
        <property type="entry name" value="SCAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0770">Synapse</keyword>
    <keyword evidence="8 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 17" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00038169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU363122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AHH40199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017341038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="RefSeq" id="XP_053541526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_053541527.1"/>
        </source>
    </evidence>
    <sequence length="230" mass="25629" checksum="64137A92A23A1C22" modified="2014-04-16" version="1">MAENNFPPLPRFIPLKPCFYQDFNEIPEQFRTMCKRLYYLWILNSATLATNLIGCLAWMCGGGGATNFGMAILWLILFTPCSYVCWFRPIYKAFKSDSSFNFMAFFFVFMAQVVISIIQAVGIPGWGVCGWLATITFFSTNIGSAVVMLIPTIMFTAVAVLSFIALTKVHNFYRGSGGSMSKAQEEWTTGAWKNPHVQQAAQQAAMGAAQGAMQGQQYSPPPTYNYNDPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>W5UFS1</accession>
    <name>W5UFS1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Zinc finger protein 593</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15 17" type="primary">znf593</name>
        <name evidence="17" type="synonym">zn593</name>
    </gene>
    <organism evidence="15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="15">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in pre-60S ribosomal particles maturation by promoting the nuclear export of the 60S ribosome. Negatively modulates the DNA binding activity of Oct-2 and therefore its transcriptional regulatory activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Associates with pre-60S ribosomal particles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ZNF593/BUD20 C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="JT409032">
        <property type="protein sequence ID" value="AHH38537.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017330779.1">
        <property type="nucleotide sequence ID" value="XM_017475290.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UFS1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022731"/>
    <dbReference type="GeneID" id="100528687"/>
    <dbReference type="CTD" id="51042"/>
    <dbReference type="GeneTree" id="ENSGT00390000004173"/>
    <dbReference type="OMA" id="MKDHFRS"/>
    <dbReference type="OrthoDB" id="24683at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000299">
        <property type="entry name" value="Zinc finger protein 593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051879">
        <property type="entry name" value="C2H2-ZF_Maturation_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46095">
        <property type="entry name" value="ZINC FINGER PROTEIN 593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46095:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN 593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="95"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AHH38537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017330779.1"/>
        </source>
    </evidence>
    <sequence length="129" mass="14929" checksum="26B47D2A7193EB1D" modified="2014-04-16" version="1">MGKSKQVGNHKNGRKKNIAKTWKTKRRTKDLDQIHADMIPANAAKLLKQEVDYDVTGFGQHYCLHCARYFVDLKAMKEHFKTKVHKKRLKELREEPYTQAEAERAAGMGSYVPPKKVEVQTQEVEEDMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>W5UIL6</accession>
    <accession>A0A2D0QL17</accession>
    <name>W5UIL6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3 5">Regulator of G-protein signaling 17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">RGS17</name>
        <name evidence="5" type="synonym">rgs17</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2023-07" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT411626">
        <property type="protein sequence ID" value="AHH39522.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017318406.2">
        <property type="nucleotide sequence ID" value="XM_017462917.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UIL6"/>
    <dbReference type="GeneID" id="108262465"/>
    <dbReference type="CTD" id="26575"/>
    <dbReference type="GeneTree" id="ENSGT00940000155393"/>
    <dbReference type="OrthoDB" id="10266999at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000001">
        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845">
        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845:SF196">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RGS" evidence="2">
        <location>
            <begin position="83"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AHH39522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017318406.2"/>
        </source>
    </evidence>
    <sequence length="208" mass="24260" checksum="D060D8F414F8773F" modified="2014-04-16" version="1">MPQNVSGVEMRKQQQAHIEGPPQAPGHPRPNTCCLCWCGCCKCLWNEENMEHPERQTCTKMDSIEATEEQHPTLDEVVAWARSFELMMRSSEGRDIFRQFLRSEYSEENLMFWIACEELKKEANPTAIDEKARIIYEDYVSILSPKEVSLDSRVREGINQSLAEPSSLMYEEAQLQIYTLMHRDSFPRFLNSSVYRDLLERKRACLDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>W5UK58</accession>
    <name>W5UK58_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="16 18">Krueppel-like factor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">Klf2</name>
        <name evidence="18" type="synonym">klf2b</name>
    </gene>
    <organism evidence="16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="16">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SDA103</strain>
        </source>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="18">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="JT411951">
        <property type="protein sequence ID" value="AHH39673.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JT417925">
        <property type="protein sequence ID" value="AHH42554.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017351857.1">
        <property type="nucleotide sequence ID" value="XM_017496368.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UK58"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009560"/>
    <dbReference type="GeneID" id="108280879"/>
    <dbReference type="KEGG" id="ipu:108280879"/>
    <dbReference type="CTD" id="117509"/>
    <dbReference type="GeneTree" id="ENSGT00940000164495"/>
    <dbReference type="OMA" id="ASDCQAQ"/>
    <dbReference type="OrthoDB" id="4748970at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21583">
        <property type="entry name" value="KLF2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000018">
        <property type="entry name" value="Krueppel-like factor 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000237">
        <property type="entry name" value="Krueppel-like factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF154">
        <property type="entry name" value="KRUEPPEL-LIKE FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="314"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="344"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="374"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AHH42554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="RefSeq" id="XP_017351857.1"/>
        </source>
    </evidence>
    <sequence length="397" mass="44034" checksum="1C7937BD7B9ED668" modified="2014-04-16" version="1">MALPGTILPSISTFAGQKEKLWENRWIGELEKPLACAVPSVDVCRARKDDEDLSDFLDLEFILANTTGPESVMGPADYNSAVEHCSMFQTGSAYVPPPPPYSHSFVAELLHSDADNTYCMSMSTSTTTSTTSSNSNSIGIQGRFVVNSAPFPRQDFVEGIKVEPCSDGYGPVMGMVPQSCHRIKQEGNVSCAMPFDQPRLAVSPQAAGNMTPPLSPDDQARCHTQICRTTFPQNGYYPSHAPLPQQQQQHQHQQVPLAYAGARAYAGMFDDGLGMVQPGAPRALLTPPSSPLELLETKPKRGRRTWPRKRAATHTCTFAGCGKTYTKSSHLKAHHRTHTGEKPYHCNWEGCGWKFARSDELTRHFRKHTGHRPFQCHLCERAFSRSDHLALHMKRHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>W5UK72</accession>
    <name>W5UK72_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial inner membrane protease subunit</fullName>
            <ecNumber evidence="12">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 16" type="primary">immp2l</name>
    </gene>
    <organism evidence="14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="14">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterodimer of 2 subunits, IMMPL1 and IMMPL2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S26 family. IMP2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="12"/>
    <dbReference type="EMBL" id="JT416626">
        <property type="protein sequence ID" value="AHH42100.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017329299.1">
        <property type="nucleotide sequence ID" value="XM_017473810.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UK72"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009822"/>
    <dbReference type="GeneID" id="108268701"/>
    <dbReference type="KEGG" id="ipu:108268701"/>
    <dbReference type="CTD" id="83943"/>
    <dbReference type="GeneTree" id="ENSGT00550000075044"/>
    <dbReference type="OMA" id="YILWPPS"/>
    <dbReference type="OrthoDB" id="9996127at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042720">
        <property type="term" value="C:mitochondrial inner membrane peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.109.10:FF:000005">
        <property type="entry name" value="Mitochondrial inner membrane protease subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.109.10">
        <property type="entry name" value="Umud Fragment, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037730">
        <property type="entry name" value="IMP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000223">
        <property type="entry name" value="Pept_S26A_signal_pept_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019758">
        <property type="entry name" value="Pept_S26A_signal_pept_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02227">
        <property type="entry name" value="sigpep_I_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46041">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46041:SF2">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00727">
        <property type="entry name" value="LEADERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00761">
        <property type="entry name" value="SPASE_I_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4 12 14" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S26" evidence="13">
        <location>
            <begin position="14"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S26" evidence="13">
        <location>
            <begin position="108"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR600223-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AHH42100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017329299.1"/>
        </source>
    </evidence>
    <sequence length="188" mass="21039" checksum="8BA2F7029CD4E9C9" modified="2014-04-16" version="1">MAQTAGLGRKYLKAFVSGFFVAVPVTVTVLDRLAYVARVEGASMQPSLNPQGALSSDVVLLNRWSVRNYEVQRGDIVSVVSPKNPKQKIIKRVIALEGDFIKTMGYKNRYVRVPDGHLWIEGDHHGHSFDSNTFGPVSLGLLHGRASHIIWPPNRWQRIRPCVPPDRRPLLNWGGSTEDDSNDDNNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>W5UKB7</accession>
    <name>W5UKB7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3 5">Myocardial zonula adherens protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">myzap</name>
    </gene>
    <organism evidence="3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="3">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="EMBL" id="JT418108">
        <property type="protein sequence ID" value="AHH42619.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017314310.1">
        <property type="nucleotide sequence ID" value="XM_017458821.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5UKB7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000034407"/>
    <dbReference type="GeneID" id="108259377"/>
    <dbReference type="KEGG" id="ipu:108259377"/>
    <dbReference type="CTD" id="100820829"/>
    <dbReference type="OrthoDB" id="8788688at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051375">
        <property type="entry name" value="Tuftelin_GRINL1A/MYZAP/CCD68"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23171:SF3">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23171">
        <property type="entry name" value="GDOWN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="504"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="213"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="68"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="532"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AHH42619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017314310.1"/>
        </source>
    </evidence>
    <sequence length="569" mass="64983" checksum="8FFDFA35D94DE29A" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>W5ULB9</accession>
    <name>W5ULB9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="16">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">Zap70</name>
        <name evidence="26 27 28 29" type="synonym">LOC108264986</name>
    </gene>
    <organism evidence="24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="24">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">SDA103</strain>
        </source>
    </reference>
    <reference evidence="26 27 28" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="26 27 28">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="16"/>
    <dbReference type="EMBL" id="JT414060">
        <property type="protein sequence ID" value="AHH40482.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017322509.1">
        <property type="nucleotide sequence ID" value="XM_017467020.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017322510.1">
        <property type="nucleotide sequence ID" value="XM_017467021.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017322511.1">
        <property type="nucleotide sequence ID" value="XM_017467022.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017322512.1">
        <property type="nucleotide sequence ID" value="XM_017467023.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ULB9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021172"/>
    <dbReference type="GeneID" id="108264986"/>
    <dbReference type="KEGG" id="ipu:108264986"/>
    <dbReference type="OMA" id="ACPNTSA"/>
    <dbReference type="OrthoDB" id="535945at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09938">
        <property type="entry name" value="SH2_N-SH2_Zap70_Syk_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.930.10:FF:000001">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000185">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000031">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000216">
        <property type="entry name" value="Tyrosine-protein kinase SYK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.930.10">
        <property type="entry name" value="Syk Kinase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023420">
        <property type="entry name" value="Kinase_SYK/ZAP-70_inter-SH2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035838">
        <property type="entry name" value="SYK/ZAP-70_N_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
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    <dbReference type="InterPro" id="IPR012234">
        <property type="entry name" value="Tyr_kinase_non-rcpt_SYK/ZAP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000604">
        <property type="entry name" value="TyrPK_SYK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="10 16 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0391">Immunity</keyword>
    <keyword evidence="9 16 24" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0519">Myristate</keyword>
    <keyword evidence="8 16 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 19" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="5 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="14 16" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="SH2" evidence="22">
        <location>
            <begin position="10"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="22">
        <location>
            <begin position="163"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="328"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="258"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="451"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="334"/>
            <end position="342"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18 20">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000604-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000604-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="AHH40482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="RefSeq" id="XP_017322509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="RefSeq" id="XP_017322510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="RefSeq" id="XP_017322511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="RefSeq" id="XP_017322512.1"/>
        </source>
    </evidence>
    <sequence length="607" mass="69085" checksum="365729546703FE80" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>W5ULX1</accession>
    <name>W5ULX1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17 19" type="primary">mylipa</name>
    </gene>
    <organism evidence="17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="17">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC). Regulates cell movements during gastrulation by acting downstream of fz7 to antagonize the frizzled-signaling pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with anxa5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JT414626">
        <property type="protein sequence ID" value="AHH40697.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017337855.1">
        <property type="nucleotide sequence ID" value="XM_017482366.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W5ULX1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000025049"/>
    <dbReference type="GeneID" id="108273225"/>
    <dbReference type="KEGG" id="ipu:108273225"/>
    <dbReference type="CTD" id="335888"/>
    <dbReference type="GeneTree" id="ENSGT00940000156206"/>
    <dbReference type="OMA" id="NKGENLW"/>
    <dbReference type="OrthoDB" id="10037309at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007369">
        <property type="term" value="P:gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13195">
        <property type="entry name" value="FERM_C_MYLIP_IDOL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17104">
        <property type="entry name" value="FERM_F1_MYLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16523">
        <property type="entry name" value="RING-HC_MYLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000019">
        <property type="entry name" value="E3 ubiquitin-protein ligase MYLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000129">
        <property type="entry name" value="E3 ubiquitin-protein ligase MYLIP isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000164">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase MYLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000175">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase MYLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041790">
        <property type="entry name" value="MYLIP_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280">
        <property type="entry name" value="4.1 G PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280:SF13">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MYLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="11" id="KW-0306">Gastrulation</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FERM" evidence="15">
        <location>
            <begin position="1"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="384"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00062023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AHH40697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017337855.1"/>
        </source>
    </evidence>
    <sequence length="472" mass="53361" checksum="9502A718A7EF4547" modified="2014-04-16" version="1">MLCHVTRPDSVVMEVEVDAKANGEDCLNKVCRKLGVIEVDYFGLQFSGSKGENLWLNLRNRISQQMDSVTPCRLRLRVKFFVEPHLILQEQTRHLFFMHVKEDLHRGHLRMCSEKAEELSALLAQAEFGDYNQNTAKYWYTELCRKDPDQETVNSIIAKHKALEGLSQGSVEYQALQLVASLEHYGVEWHWARDAEGQRLAVGVGPDGIAICRDDFSLVNRISYPLIQIATQSGKSVFLTVTKESSDSVVLLFKLISNRAASGLYRAITETHAFYRCDTVTNAVMMQYSRDFKGHLASLFLNENINLGKKYVFDIRRTSKEVYDYARRALYNAGIVDLMSRSGARSPSSRSPWHGQENAPECGGCRQNRLLQEKLQKLREAFLCMLCCEEEIDAAFCPCGHTVCCQNCAAQLQSCPVCRSEVEHIQHVYLPTCSSLLNLTVCSDSPAPIHRSMATHTCTTSHYCSDDKLCQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2014-04-16" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>W6A3K2</accession>
    <name>W6A3K2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Disks large homolog 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">DLG1</name>
        <name evidence="23" type="synonym">dlg1b</name>
    </gene>
    <organism evidence="21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2012" name="BMC Genomics" volume="13" first="595" last="595">
            <title>Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.</title>
            <authorList>
                <person name="Liu S."/>
                <person name="Zhang Y."/>
                <person name="Zhou Z."/>
                <person name="Waldbieser G."/>
                <person name="Sun F."/>
                <person name="Lu J."/>
                <person name="Zhang J."/>
                <person name="Jiang Y."/>
                <person name="Zhang H."/>
                <person name="Wang X."/>
                <person name="Rajendran K.V."/>
                <person name="Khoo L."/>
                <person name="Kucuktas H."/>
                <person name="Peatman E."/>
                <person name="Liu Z."/>
            </authorList>
            <dbReference type="PubMed" id="23127152"/>
            <dbReference type="DOI" id="10.1186/1471-2164-13-595"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <tissue evidence="21">Mixed</tissue>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">SDA103</strain>
        </source>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="23">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="EMBL" id="JT417715">
        <property type="protein sequence ID" value="AHI50450.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053529560.1">
        <property type="nucleotide sequence ID" value="XM_053673585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="W6A3K2"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035255">
        <property type="term" value="F:ionotropic glutamate receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097120">
        <property type="term" value="P:receptor localization to synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099072">
        <property type="term" value="P:regulation of postsynaptic membrane neurotransmitter receptor levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06723">
        <property type="entry name" value="PDZ1_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06724">
        <property type="entry name" value="PDZ2_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06795">
        <property type="entry name" value="PDZ3_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12031">
        <property type="entry name" value="SH3_DLG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001402">
        <property type="entry name" value="Discs, large homolog 3 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.470:FF:000001">
        <property type="entry name" value="Disks large 1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000008">
        <property type="entry name" value="Disks large homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000001">
        <property type="entry name" value="Disks large homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000001">
        <property type="entry name" value="Disks large homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000058">
        <property type="entry name" value="Disks large homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000049">
        <property type="entry name" value="disks large homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000002">
        <property type="entry name" value="Disks large homolog 4 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.10">
        <property type="entry name" value="Guanylate Kinase phosphate binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.470">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019583">
        <property type="entry name" value="DLG1-4_PDZ_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016313">
        <property type="entry name" value="DLG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019590">
        <property type="entry name" value="DLG1_PEST_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015143">
        <property type="entry name" value="L27_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036892">
        <property type="entry name" value="L27_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050614">
        <property type="entry name" value="Synaptic_Scaffolding_LAP-MAGUK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119">
        <property type="entry name" value="DISCS LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119:SF5">
        <property type="entry name" value="DISKS LARGE HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09058">
        <property type="entry name" value="L27_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10608">
        <property type="entry name" value="MAGUK_N_PEST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10600">
        <property type="entry name" value="PDZ_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001741">
        <property type="entry name" value="MAGUK_DLGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00569">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01277">
        <property type="entry name" value="MAGUK_N_PEST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101288">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51022">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="12" id="KW-0965">Cell junction</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 15" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="L27" evidence="20">
        <location>
            <begin position="4"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="19">
        <location>
            <begin position="223"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="19">
        <location>
            <begin position="318"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="19">
        <location>
            <begin position="467"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="582"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="18">
        <location>
            <begin position="715"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="429"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="658"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="429"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="682"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="AHI50450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="RefSeq" id="XP_053529560.1"/>
        </source>
    </evidence>
    <sequence length="905" mass="100335" checksum="3EBD4743ABFA77F1" modified="2014-04-16" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PHT5</accession>
    <name>A0A2D0PHT5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sulfotransferase</fullName>
            <ecNumber evidence="15">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC108254972</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="15"/>
    <dbReference type="RefSeq" id="XP_017306008.1">
        <property type="nucleotide sequence ID" value="XM_017450519.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PHT5"/>
    <dbReference type="GeneID" id="108254972"/>
    <dbReference type="KEGG" id="ipu:108254972"/>
    <dbReference type="OMA" id="QRFYQMS"/>
    <dbReference type="OrthoDB" id="411451at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008467">
        <property type="term" value="F:[heparan sulfate]-glucosamine 3-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000194">
        <property type="entry name" value="Sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037359">
        <property type="entry name" value="NST/OST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605:SF62">
        <property type="entry name" value="HEPARAN SULFATE GLUCOSAMINE 3-O-SULFOTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605">
        <property type="entry name" value="HEPARAN SULFATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="17">
        <location>
            <begin position="108"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="active site" description="For sulfotransferase activity" evidence="12">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="117"/>
            <end position="121"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="323"/>
            <end position="327"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="306"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017306008.1"/>
        </source>
    </evidence>
    <sequence length="360" mass="40593" checksum="24F7AB1A3A93C46D" modified="2017-12-20" version="1">MGCSVCRTRLNSGRALSRVPLSFTMLLIFLYLFHCLSGFCDSAPKITDGQNLMRDHVPGKVHAVRAELRWNGTVSAHVSALSELQESENGGGESGIPVSNTYGSKRFPQAIIIGVKKGGTRALLEFLRVHPDVRAVGAEPHFFDRFYHKGLEWYRNLMPRTLDGQITMEKTPSYFVTKEAPSRLCAMNCDTKLIVVVRDPVTRAVSDYTQTRTKSPGLQSFQTLVFKNCSLGVIDTSWSAVRIGMYAQHLENWLQHFPLSSFLFVSGERLVSDPAGEVGRVQDFLGLKRIVSDKHFYFNQTKGFPCLKKPEGSSRPRCLGKSKGRTHPHIPTDVLQKLREFYRPLNHKFYQMSGLDFGWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PJL8</accession>
    <name>A0A2D0PJL8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 12">Abasic site processing protein HMCES</fullName>
            <shortName evidence="12">ES cell-specific 5hmC-binding protein</shortName>
            <ecNumber evidence="12">3.4.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 12">Embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10 12">Peptidase HMCES</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 12">SRAP domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15 16 17" type="primary">hmces</name>
    </gene>
    <organism evidence="14 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15 16 17" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15 16 17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites. Acts as an enzyme that recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA: forms a stable thiazolidine linkage between a ring-opened abasic site and the alpha-amino and sulfhydryl substituents of its N-terminal catalytic cysteine residue. The HMCES DNA-protein cross-link is then either reversed or degraded. HMCES is able to catalyze the reversal of its thiazolidine cross-link and cycle between a cross-link and a non-cross-linked state depending on DNA context: mediates self-reversal of the thiazolidine cross-link in double stranded DNA, allowing APEX1 to initiate downstream repair of abasic sites. The HMCES DNA-protein cross-link can also be degraded by the SPRTN metalloprotease following unfolding by the BRIP1/FANCJ helicase. Acts as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the SOS response-associated peptidase family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="12"/>
    <dbReference type="RefSeq" id="XP_017305574.1">
        <property type="nucleotide sequence ID" value="XM_017450085.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017305575.1">
        <property type="nucleotide sequence ID" value="XM_017450086.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017305576.1">
        <property type="nucleotide sequence ID" value="XM_017450087.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PJL8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000033376"/>
    <dbReference type="GeneID" id="108254779"/>
    <dbReference type="KEGG" id="ipu:108254779"/>
    <dbReference type="CTD" id="56941"/>
    <dbReference type="GeneTree" id="ENSGT00390000018439"/>
    <dbReference type="OrthoDB" id="2111841at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106300">
        <property type="term" value="P:protein-DNA covalent cross-linking repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1680.10">
        <property type="entry name" value="SOS response associated peptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003738">
        <property type="entry name" value="SRAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036590">
        <property type="entry name" value="SRAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604:SF0">
        <property type="entry name" value="ABASIC SITE PROCESSING PROTEIN HMCES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604">
        <property type="entry name" value="DC12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02586">
        <property type="entry name" value="SRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143081">
        <property type="entry name" value="BB1717-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0190">Covalent protein-DNA linkage</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 12" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="18"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="146"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="276"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="285"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="311"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017305574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017305575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017305576.1"/>
        </source>
    </evidence>
    <sequence length="349" mass="39463" checksum="749746CA5B475A9A" modified="2017-12-20" version="1">MCGRTACTLAPDELRRASRYRDRSGQRRQPQWRDGDADKYRPSYNKSPQSFSPVLLSSRHFSKEAAVDECVLATMRWGLVPAWFKESDPSKMQYSTSNCRSESLLEKKSYKDPLLKGQRCVILADGFYEWRRQQKDKQPFFIYFPQSQTDNRDGDERETKNGVRHEGQERDAEESGEWAGWRLLTIAGLFDCWTPPGGGEVLYTYTVITVNASPNLQSIHDRMPAILDGEEEVRRWLDFGEVRSLEALKLLQSKSCLTFHPVSSIVNNARNNSPECLQPLDPNAKKAPPQASASSKMMMSWLKNGSPGKKKAPDDAELKETAGKSPTSKAVGPLRQWLLGNGASKRPRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PJN8</accession>
    <name>A0A2D0PJN8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Macrophage mannose receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108255385</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017306813.1">
        <property type="nucleotide sequence ID" value="XM_017451324.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PJN8"/>
    <dbReference type="GeneID" id="108255385"/>
    <dbReference type="KEGG" id="ipu:108255385"/>
    <dbReference type="OrthoDB" id="7357196at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 20 MEMBER A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784:SF3">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 4 MEMBER K-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="1">
        <location>
            <begin position="25"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="1">
        <location>
            <begin position="152"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017306813.1"/>
        </source>
    </evidence>
    <sequence length="330" mass="38166" checksum="F4CA33E2D554101F" modified="2017-12-20" version="1">MKQRLFVLLFLTGFVPCLESVNSQYFLILEEKTWTDAQAYCRANYDDLATIAGWSDLDKIKAEAQAHKFISYAWIGLYTNTSAWRWSLGNQSLGNMTDWYTYPPNSEYNEPNNANGHEECCAFNPLGWFDRSCGEQFGFVCFDNQKKGTGKYIYYSTPLTWNAAQSYCRQYHTDLTSSRDATENSIIMNTIPQNNSFNWFGLFRNSWMWSDGNNSTFMVWAQHQPKGNENCVNIRNSRGYNSPCSDVLPFFCHAVAYPRKRQIIRMKVRSDQDVNNPAVLLAILQKIQQKMNDHGIANTTLKWRETLDGEVFHKEKENTTTSRTNTTCDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PK82</accession>
    <name>A0A2D0PK82_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Voltage-dependent calcium channel subunit alpha-2/delta-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">cacna2d3</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">SDA103</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="21">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calcium channel subunit alpha-2/delta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017305465.2">
        <property type="nucleotide sequence ID" value="XM_017449976.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PK82"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000031066"/>
    <dbReference type="GeneID" id="108254724"/>
    <dbReference type="KEGG" id="ipu:108254724"/>
    <dbReference type="CTD" id="55799"/>
    <dbReference type="OrthoDB" id="10054666at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01463">
        <property type="entry name" value="vWA_VGCC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000012">
        <property type="entry name" value="Calcium channel, voltage-dependent, alpha2/delta subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000007">
        <property type="entry name" value="Calcium voltage-gated channel auxiliary subunit alpha2delta 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051173">
        <property type="entry name" value="Ca_channel_alpha-2/delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013680">
        <property type="entry name" value="VDCC_a2/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013608">
        <property type="entry name" value="VWA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL SUBUNIT ALPHA-2/DELTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10166:SF56">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL SUBUNIT ALPHA-2_DELTA-3 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08473">
        <property type="entry name" value="VGCC_alpha2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08399">
        <property type="entry name" value="VWA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Liu Z."/>
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                <person name="Bao L."/>
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                <person name="Zhang Y."/>
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                <person name="Gao S."/>
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                <person name="Li C."/>
                <person name="Wang X."/>
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                <person name="Yuan Z."/>
                <person name="Yang Y."/>
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                <person name="Lu J."/>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Geng X."/>
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                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
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                <person name="Yuan Z."/>
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                <person name="Peatman E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">SDA103</strain>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050868">
        <property type="term" value="P:negative regulation of T cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032748">
        <property type="entry name" value="PAG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16322">
        <property type="entry name" value="PHOSPHOPROTEIN ASSOCIATED WITH GLYCOSPHINGOLIPID-ENRICHED MICRODOMAINS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16322:SF0">
        <property type="entry name" value="PHOSPHOPROTEIN ASSOCIATED WITH GLYCOSPHINGOLIPID-ENRICHED MICRODOMAINS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15347">
        <property type="entry name" value="PAG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017309798.1"/>
        </source>
    </evidence>
    <sequence length="381" mass="40606" checksum="5A5095874FBD2857" modified="2017-12-20" version="1">MAPALSALWGSGVAGSEISGELAIVSTLTAFWAFLLLTLLLVLCAGCQGHKKATQLPGDHENLMNGVSEKETCSQTADTGSHATDLVASSSFNGPLTSGTVLTDTVDTSPHPSEEMVSIQSEHRSSKCHQDRELPSIPCANSLRDAAEASEECRAGDSTYEVLKDGASHDAVEDSPYETVKELKEACLTNGTLTPDEPPTAKPNGHLNASPGTPDLCPLADGAEYASIDLKKKSRYSVDAEAQRSAVEQPVEEENPPPIPDKVLDENDNQNITTLQNGELSALYSTVDKPAKEEENEPDYSSIGEIRGIVVESSSSELYATVRDVYPSPPAEQPAENTDQGYETIKIAKSVPDEGQQDNGLLESDYANVGELELNSETSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PVP8</accession>
    <name>A0A2D0PVP8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Leucine-rich repeat-containing protein 14B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">lrrc14b</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PRAME family. LRRC14 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017310114.1">
        <property type="nucleotide sequence ID" value="XM_017454625.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PVP8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029068"/>
    <dbReference type="GeneID" id="108257144"/>
    <dbReference type="KEGG" id="ipu:108257144"/>
    <dbReference type="CTD" id="389257"/>
    <dbReference type="OMA" id="KACYLHE"/>
    <dbReference type="OrthoDB" id="8875973at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045596">
        <property type="term" value="P:negative regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000313">
        <property type="entry name" value="Leucine rich repeat containing 14B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050694">
        <property type="entry name" value="LRRC14/PRAME"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026271">
        <property type="entry name" value="PRAME"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14224:SF27">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 14B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14224">
        <property type="entry name" value="SIMILAR TO PREFERENTIALLY EXPRESSED ANTIGEN IN MELANOMA-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038286">
        <property type="entry name" value="PRAME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00067566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017310114.1"/>
        </source>
    </evidence>
    <sequence length="524" mass="59028" checksum="38E8F2794751A561" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PXW0</accession>
    <name>A0A2D0PXW0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Endoplasmic reticulum protein SC65</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Leprecan-like protein 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Prolyl 3-hydroxylase family member 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Synaptonemal complex protein SC65</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">p3h4</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linking of collagen fibrils. Required for normal bone density and normal skin stability via its role in hydroxylation of lysine residues in collagen alpha chains and in collagen fibril assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with PLOD1, P3H3 and PPIB. Identified in a complex with PLOD1 and P3H3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the leprecan family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017310266.1">
        <property type="nucleotide sequence ID" value="XM_017454777.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PXW0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020922"/>
    <dbReference type="GeneID" id="108257212"/>
    <dbReference type="KEGG" id="ipu:108257212"/>
    <dbReference type="CTD" id="10609"/>
    <dbReference type="OMA" id="EAFCGRN"/>
    <dbReference type="OrthoDB" id="8610171at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005518">
        <property type="term" value="F:collagen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030199">
        <property type="term" value="P:collagen fibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000119">
        <property type="entry name" value="synaptonemal complex protein SC65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052284">
        <property type="entry name" value="Collagen_mod_leprecan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056585">
        <property type="entry name" value="Leprecan_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13986:SF4">
        <property type="entry name" value="ENDOPLASMIC RETICULUM PROTEIN SC65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13986">
        <property type="entry name" value="PROTEIN LYSINE HYDROXYLATION COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23557">
        <property type="entry name" value="TPR_leprecan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012067594" description="Endoplasmic reticulum protein SC65" evidence="13">
        <location>
            <begin position="22"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Leprecan-like alpha-helical" evidence="14">
        <location>
            <begin position="38"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="374"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00078679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017310266.1"/>
        </source>
    </evidence>
    <sequence length="421" mass="49217" checksum="22CBF846013BE6DC" modified="2017-12-20" version="1">MANVYVCVLLCAAVLVPAVRCQYEHYSAENFPQADLMPLDSAYGFALERYGAREWVESARYLELSLRLHRLLKESEAHCSRACTRDNLHDTDMDTSLQIFRHILTHAACVKKCKSEMPVFSKQYPNPETLEAFSTRTPYRYLQFAYYQMNNLEKAISAAHTYLQRNPGDPLISKNMNFYKSLFDIEEYLMDQEEKKHETLFLKAVLLYNAGDFSRSVSMMEEVCVEYLRVYDECVSACEGSYEVQEVKDFYHTLAGFFMEVMRCKMRCEENLTPNVGGFFVEKFVATIYHYMQFAYYKLNDVIKAAPCALSYLLFDPDDQVMQQNVAYYKFHRNQWSLSEEEFKPRPEALRYFNQTMKQKELLKFARNYLQTDDEDVVSPEESPALSSPDEEFDGVGDYEESFLAEWWQEPKTKGDTGEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PYF4</accession>
    <name>A0A2D0PYF4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Homeobox protein SIX6b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">six6b</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SIX/Sine oculis homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017311067.1">
        <property type="nucleotide sequence ID" value="XM_017455578.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PYF4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000025798"/>
    <dbReference type="GeneID" id="108257662"/>
    <dbReference type="KEGG" id="ipu:108257662"/>
    <dbReference type="CTD" id="553611"/>
    <dbReference type="OMA" id="FTKDSHS"/>
    <dbReference type="OrthoDB" id="3501850at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043010">
        <property type="term" value="P:camera-type eye development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008283">
        <property type="term" value="P:cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000046">
        <property type="entry name" value="SIX homeobox 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031701">
        <property type="entry name" value="SIX1_SD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10390">
        <property type="entry name" value="HOMEOBOX PROTEIN SIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10390:SF12">
        <property type="entry name" value="HOMEOBOX PROTEIN SIX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16878">
        <property type="entry name" value="SIX1_SD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="126"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="128"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="198"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017311067.1"/>
        </source>
    </evidence>
    <sequence length="242" mass="27397" checksum="869DF73D17EC50A1" modified="2017-12-20" version="1">MFQLPMLNFSPQQVAGVCETLEESGDIERLGRFLWSLPVAPAACEVLNRNESVLRARAIVAFHTGNFRELYHILENHKFTKDSHSKLQALWLESHYREAEKLRGRPLGPVDKYRVRKKFPLPKTIWDGEQKTHCFKERTRHLLREWYLQDPYPNPSKKRELAQATGLTPTQVGNWFKNRRQRDRAAAAKNRLQQQVLTNGSVTAEDGAADRLGNASSPEAGLSSQAAASAISITSSDSECDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0PYR4</accession>
    <name>A0A2D0PYR4_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5 6">Protein FAM102B isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">fam102bb</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5 6" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5 6">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EEIG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017311178.1">
        <property type="nucleotide sequence ID" value="XM_017455689.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053531577.1">
        <property type="nucleotide sequence ID" value="XM_053675602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0PYR4"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000030580"/>
    <dbReference type="GeneID" id="108257723"/>
    <dbReference type="KEGG" id="ipu:108257723"/>
    <dbReference type="CTD" id="557909"/>
    <dbReference type="GeneTree" id="ENSGT00940000156132"/>
    <dbReference type="OMA" id="NRYPVKQ"/>
    <dbReference type="OrthoDB" id="3365224at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039931">
        <property type="entry name" value="EEIG1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21456:SF3">
        <property type="entry name" value="EEIG FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21456">
        <property type="entry name" value="FAMILY WITH SEQUENCE SIMILARITY 102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10358">
        <property type="entry name" value="NT-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51840">
        <property type="entry name" value="C2_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 NT-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="204"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="213"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="289"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017311178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_053531577.1"/>
        </source>
    </evidence>
    <sequence length="368" mass="39519" checksum="CD2E587942D6807C" modified="2017-12-20" version="1">MMKKKKFKFKVDFELDELSSVPFVNGVLFCKIRLLDGGFSEESSREMVQANCVRWRTRFSFPCKMSASAGTGVLDPCICRVSVRKELKGGKTYAKLGFADLNLAEFAGSGSTTRRCLLEGYDTKNTRQDNSILKVIICTHLMSGDPCFKTPPSTATVIGIQGNEESLLEERRGGETQKPFLGLSETTGKCGSLSEDLGVCGHSRTSSYASQQSKVSGYSSGHSRSSSLSEFTHKRNTSIDSASTGIASIPEPSDESPSSTAPAAAGLGACSTLSVNPATPDRTSSRHPMKQDSMESQLKRVDATRVDADDVVEKILQSQDFTPGLFDSSAEEEGLRLFVGPGGSTALGSHHLPVRVGAGAYEQVVIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q122</accession>
    <name>A0A2D0Q122_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">YLP motif-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Nuclear protein ZAP3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ylpm1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Plays a role in the reduction of telomerase activity during differentiation of embryonic stem cells by binding to the core promoter of TERT and controlling its down-regulation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with PPP1CA and NCOA5. Forms a complex with ILF2, ILF3, KHDRBS1, RBMX, NCOA5 and PPP1CA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017311070.1">
        <property type="nucleotide sequence ID" value="XM_017455581.3"/>
    </dbReference>
    <dbReference type="GeneID" id="108257664"/>
    <dbReference type="KEGG" id="ipu:108257664"/>
    <dbReference type="CTD" id="56252"/>
    <dbReference type="OrthoDB" id="513595at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032204">
        <property type="term" value="P:regulation of telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000399">
        <property type="entry name" value="YLP motif containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058903">
        <property type="entry name" value="Spectrin_YLPM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026314">
        <property type="entry name" value="YLP_motif_con_p1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13413">
        <property type="entry name" value="YLP MOTIF CONTAINING PROTEIN NUCLEAR PROTEIN ZAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13413:SF0">
        <property type="entry name" value="YLP MOTIF-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13671">
        <property type="entry name" value="AAA_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26583">
        <property type="entry name" value="Spectrin_YLPM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="YLPM1-like spectrin repeat" evidence="14">
        <location>
            <begin position="292"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="82"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="215"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="404"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="829"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1311"/>
            <end position="1475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="130"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="141"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="220"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="438"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="462"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="485"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="557"/>
            <end position="578"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="589"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="652"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="674"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="686"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="709"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="829"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="853"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="950"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1002"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1027"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1076"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1116"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1150"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1320"/>
            <end position="1380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1388"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1453"/>
            <end position="1467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00065932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF26583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017311070.1"/>
        </source>
    </evidence>
    <sequence length="1786" mass="201648" checksum="1A7CC17115B17A91" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q222</accession>
    <name>A0A2D0Q222_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Calcyon neuron-specific vesicular protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">caly</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NSG family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017456853.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A2D0Q222"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000022658"/>
    <dbReference type="GeneID" id="108258305"/>
    <dbReference type="KEGG" id="ipu:108258305"/>
    <dbReference type="CTD" id="50632"/>
    <dbReference type="OMA" id="ILKQKHC"/>
    <dbReference type="OrthoDB" id="9866545at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032051">
        <property type="term" value="F:clathrin light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009431">
        <property type="entry name" value="NSG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28546:SF1">
        <property type="entry name" value="NEURON-SPECIFIC VESICULAR PROTEIN CALCYON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28546">
        <property type="entry name" value="NEURONAL VESICLE TRAFFICKING-ASSOCIATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06387">
        <property type="entry name" value="Calcyon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017312342.1"/>
        </source>
    </evidence>
    <sequence length="244" mass="27769" checksum="3E785D9B847B3470" modified="2017-12-20" version="1">MRSAKRRRSLRRIRHCHGNCATSFSRTDFLFLFLFSIFLKSLPHGTARPLPTQKTDMVKLGSTLGEKVERESSLEDGFDNIPLITPLEVNQLQKPFPDKVIVKTTTEYQLQKKQKKKMYMPSIKKLNINLYDEVSEKVKLTGLILITLAFLACLLLLVMYKALWYDQLSCPEGFILKHKHCTPAALEMYYPEQDAGSRGSLYTAISHLNPAKKALPELSSPWLPVINAMKESKKAKGEASHSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q383</accession>
    <name>A0A2D0Q383_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">E3 ubiquitin/ISG15 ligase TRIM25</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC108258555</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017312751.1">
        <property type="nucleotide sequence ID" value="XM_017457262.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q383"/>
    <dbReference type="GeneID" id="108258555"/>
    <dbReference type="KEGG" id="ipu:108258555"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16040">
        <property type="entry name" value="SPRY_PRY_SNTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF5">
        <property type="entry name" value="E3 UBIQUITIN_ISG15 LIGASE TRIM25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017312751.1"/>
        </source>
    </evidence>
    <sequence length="561" mass="64233" checksum="8AF737851E6206F0" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q6Z8</accession>
    <name>A0A2D0Q6Z8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Crystallin beta gamma X</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">crybgx</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017314468.1">
        <property type="nucleotide sequence ID" value="XM_017458979.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q6Z8"/>
    <dbReference type="GeneID" id="108259467"/>
    <dbReference type="KEGG" id="ipu:108259467"/>
    <dbReference type="CTD" id="553474"/>
    <dbReference type="OMA" id="CYDRWCA"/>
    <dbReference type="OrthoDB" id="8603363at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000003">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF15">
        <property type="entry name" value="BETAGAMMAX-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="4">
        <location>
            <begin position="30"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="4">
        <location>
            <begin position="71"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="4">
        <location>
            <begin position="122"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="4">
        <location>
            <begin position="163"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017314468.1"/>
        </source>
    </evidence>
    <sequence length="207" mass="23839" checksum="2C4AFDF0F053C859" modified="2017-12-20" version="1">MNIFTKVPGIAQQTSKLGSVLQRAFYGSSGKVTLFEQRNFAGQRLDLSSDCQKLSDKNFPERCNSVQVESGAWVGYEHEHFRGRQYLWDMSDRGEYNCTDRWCGQGDHISSVRSVKQDTNPPRAQLFERQGFSGRKIEIHDDIPNLMTRYNLNRTSSIRVLGGAWVVYQEPNYRGSHYILERKDYNNFSDWGAQNSAVGSMRRICFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0Q8C1</accession>
    <name>A0A2D0Q8C1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Keratin 97</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">krt97</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017314549.1">
        <property type="nucleotide sequence ID" value="XM_017459060.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0Q8C1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013136"/>
    <dbReference type="GeneID" id="108259507"/>
    <dbReference type="KEGG" id="ipu:108259507"/>
    <dbReference type="CTD" id="436656"/>
    <dbReference type="OMA" id="EWYDKQT"/>
    <dbReference type="OrthoDB" id="2441647at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000002">
        <property type="entry name" value="Type I keratin 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000002">
        <property type="entry name" value="Type I keratin KA11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002957">
        <property type="entry name" value="Keratin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239">
        <property type="entry name" value="INTERMEDIATE FILAMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239:SF180">
        <property type="entry name" value="KERATIN, TYPE I CYTOSKELETAL 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01248">
        <property type="entry name" value="TYPE1KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="1 8" id="KW-0416">Keratin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="6">
        <location>
            <begin position="79"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="83"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="188"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="288"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017314549.1"/>
        </source>
    </evidence>
    <sequence length="442" mass="49827" checksum="69B3C729EA964209" modified="2017-12-20" version="1">MSSASVVGFSSSSSRGFSSSSLSLSGGMYSKRISGVRAPSVYGGADGRSVRVSTYQQFGSQGNLLGASGLSLDGVSVSEKLTMQNLNDRLAAYLEKVRALEKANAQLELKIREWYEKRAPVTHDYSRFMATINDLREKIRVASLGNAKIILQIDNSKLAAEDFKLKYENELMLRQSVETDISGLRRVLDDLTMSRSDLEMQIEGLKEELIYLKKNHEEELAALRAHLSSSNVNVEVDAAPQQDLAQILSEVRSQYEGIAEKNRREMEAWYKVKFDDLNKQVKVSTQTIETHRSEITELKRTLQSLQIELQSQLSLKSALEGTLGETENRYSLQLNQLQVIINKLEAELSQVRLDIERQANDYKVLLDIKTRLELEIAEYRRLLDGEDISRKTTVTKVVEVQKPAPKPEPVVTKRVRTVIEEVIDGKVVSRTEDVDVEVLNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QB10</accession>
    <name>A0A2D0QB10_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Mitochondrial outer membrane protein SLC25A46</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">slc25a46</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017315873.1">
        <property type="nucleotide sequence ID" value="XM_017460384.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QB10"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000026350"/>
    <dbReference type="GeneID" id="108260263"/>
    <dbReference type="KEGG" id="ipu:108260263"/>
    <dbReference type="CTD" id="91137"/>
    <dbReference type="OMA" id="RQCQVNH"/>
    <dbReference type="OrthoDB" id="2403262at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090149">
        <property type="term" value="P:mitochondrial membrane fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039158">
        <property type="entry name" value="SLC25A46"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21252:SF2">
        <property type="entry name" value="MITOCHONDRIAL OUTER MEMBRANE PROTEIN SLC25A46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21252">
        <property type="entry name" value="TB1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="306"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="29"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017315873.1"/>
        </source>
    </evidence>
    <sequence length="412" mass="45291" checksum="73F4D0845A545552" modified="2017-12-20" version="1">MTSRRPDSFDGLGYRGREEPSYGGGYSGRSFNSSSNTDLQHWVTTPPDIPGSRNLHYGDSTPQSEAAPAAPGAGDEFQSAASPPSDQLNRFAGFGIGLASLFTENVLAHPCIVFRRQCQVNYHARCYHLSPLTALNVMYNVSKTQGPKALWKGMGSTFVVQGVTLGTEGIISECTPLPRELPHRWNPKQIAGHFILKGLTHVIALPFYSASLIETVQSEIIRDNPGILDCVKEGVGRVLGLGVPHSKRLLPLLTLVFPTVLHGVLHYIISSSVQKLVLYVLRRRGSTGTSPKRTEFGKETVQNMLDAYFPELIASFVANLCADVLLFPLETVLHRLHIQGTRTIIDNTDQGLEVLPINSQYEGMRDCINAIRREEGPLGFYKGFGTIVIQYSLHATVLQITKMIYSTLLQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QDM2</accession>
    <name>A0A2D0QDM2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ependymin-like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">epdl1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ependymin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017316773.1">
        <property type="nucleotide sequence ID" value="XM_017461284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QDM2"/>
    <dbReference type="GeneID" id="108260763"/>
    <dbReference type="KEGG" id="ipu:108260763"/>
    <dbReference type="CTD" id="100302470"/>
    <dbReference type="OMA" id="NQTCCKK"/>
    <dbReference type="OrthoDB" id="9942506at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001299">
        <property type="entry name" value="Ependymin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10697:SF5">
        <property type="entry name" value="EPENDYMIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10697">
        <property type="entry name" value="MAMMALIAN EPENDYMIN-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00811">
        <property type="entry name" value="Ependymin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00317">
        <property type="entry name" value="EPENDYMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00026">
        <property type="entry name" value="EPEND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013402039" evidence="2">
        <location>
            <begin position="20"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017316773.1"/>
        </source>
    </evidence>
    <sequence length="212" mass="23824" checksum="AEBF6AAFDF1AA27D" modified="2017-12-20" version="1">MQLWLCCALLVFVAGGCLAQKPRPCRSPPLLEGSLTMVTQNGGYSAYGKYAYDSILRRIRLREVVRHDNETFITDVLLLYQEHVMYLINPKNKTCQKQELTSQFHPMKIPCNATLLAQVVLGSLSAPGEGLLVNSWTGETPEEKSQYFLTFTEFGCLPVSVLFNRPKTGWVVTNFFNIIRGIKDPSVFIPPPFCKNTTLETGEKGDFFSAFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QIG5</accession>
    <name>A0A2D0QIG5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">P2Y purinoceptor 11-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC108261875</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SDA103</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="13">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017318127.3">
        <property type="nucleotide sequence ID" value="XM_017462638.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QIG5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000017395"/>
    <dbReference type="GeneID" id="108261875"/>
    <dbReference type="KEGG" id="ipu:108261875"/>
    <dbReference type="OrthoDB" id="10261452at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045031">
        <property type="term" value="F:G protein-coupled ATP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030594">
        <property type="term" value="F:neurotransmitter receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231:SF46">
        <property type="entry name" value="P2Y PURINOCEPTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24231">
        <property type="entry name" value="PURINOCEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="47"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="32"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="RefSeq" id="XP_017318127.3"/>
        </source>
    </evidence>
    <sequence length="299" mass="34249" checksum="12FBE9B82BA3E2A8" modified="2023-06-28" version="3">MVGNWNRSCNSTFQQEVLPPIYGVEMCVALVGNILALWLLVTKEKNWHMGVVFSCNLVISDIFYALTLPLLIDYYSRGRQWIFGDAVCKIERFLFSCNLYVSIYFIMCISVHRYLAIVQPIFMRKHIRPKHAKIISVFVWIFVASISSPLLYFSDVYNAKCFVYANLDKKSSLKSTYRVFMGVIGCFVPFVITFSSYFGVTMAVYKNANITSLQKKKVALIIGLVCVLYTLSFVPYHILQIVNFKLREKGKTNCYVRNGYQVSMALVCLNMCLHPILYMAVSDSIRAVCCRRSSNVSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QMR8</accession>
    <name>A0A2D0QMR8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">mRNA decay activator protein ZFP36</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Zinc finger protein 36</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC108262909</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Zinc-finger RNA-binding protein that destabilizes several cytoplasmic AU-rich element (ARE)-containing mRNA transcripts by promoting their poly(A) tail removal or deadenylation, and hence provide a mechanism for attenuating protein synthesis. Acts as a 3'-untranslated region (UTR) ARE mRNA-binding adapter protein to communicate signaling events to the mRNA decay machinery. Functions by recruiting the CCR4-NOT deadenylase complex and probably other components of the cytoplasmic RNA decay machinery to the bound ARE-containing mRNAs, and hence promotes ARE-mediated mRNA deadenylation and decay processes. Binds to 3'-UTR ARE of numerous mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Associates with the cytoplasmic CCR4-NOT deadenylase complex to trigger ARE-containing mRNA deadenylation and decay processes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017318750.2">
        <property type="nucleotide sequence ID" value="XM_017463261.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QMR8"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:ribonucleoprotein complex"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:3'-UTR-mediated mRNA destabilization"/>
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    <dbReference type="GO" id="GO:1900153">
        <property type="term" value="P:positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger protein 36, C3H1 type-like 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Zinc finger, CCCH-type"/>
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    </dbReference>
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        <property type="entry name" value="Tis11B_N"/>
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    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF174">
        <property type="entry name" value="MRNA DECAY ACTIVATOR PROTEIN ZFP36L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04553">
        <property type="entry name" value="Tis11B_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="218"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="13">
        <location>
            <begin position="256"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="218"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="256"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="288"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="328"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="439"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="328"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="458"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017318750.2"/>
        </source>
    </evidence>
    <sequence length="491" mass="53835" checksum="BB0E8BDC2F19428F" modified="2023-02-22" version="2">MLRGKRAVCVLFSLGTSRRETGSNNTNKNSNTNNSRVSSSRFRPCLELVCFPPFFSPNQSWQTKSLALHYGISVGPQMKDQLRLKTSEIEMSATILSGFYDIDMLYKDKSLNMNALHLNSMLDKKAVGAPVTASALANNCTYAQGFLRRNSASNVDCTNNGNKYSATCYSNMKESSASTAIMNKENKFRERAYSETGERSQQLQALQQKPGSQINSTRYKTELCRPFEENGACKYGEKCQFAHGYHELRSLSRHPKYKTEPCRTFHTIGFCPYGPRCHFIHNAEERRPAPANANTSVPPELKASRDPIQQQAAVAAFRDRPKLHHSLSFSGFSTQHGLDSPLTESPTSRTPPPASSSDSCRSSGPFFEDVLAPSANGAFSSFATAQDLKALLAPLAVHAHGGYQSNPAYYSNFQTAMGPPSPPYSSLGHLQALQRLSESPVFDSPPSPYDSMSDRDSYASGSSGSLSGSESPSLEAGRRLPIFSRLSISDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QNM2</accession>
    <name>A0A2D0QNM2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">S-adenosylmethionine synthase</fullName>
            <ecNumber evidence="11">2.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">mat1a</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of S-adenosylmethionine from methionine and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-methionine + ATP + H2O = S-adenosyl-L-methionine + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="11">Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 magnesium ions per subunit. The magnesium ions interact primarily with the substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the AdoMet synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="11"/>
    <dbReference type="RefSeq" id="XP_017319904.1">
        <property type="nucleotide sequence ID" value="XM_017464415.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QNM2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000007957"/>
    <dbReference type="GeneID" id="108263526"/>
    <dbReference type="KEGG" id="ipu:108263526"/>
    <dbReference type="CTD" id="4143"/>
    <dbReference type="OMA" id="CEYAFRH"/>
    <dbReference type="OrthoDB" id="5852090at2759"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18079">
        <property type="entry name" value="S-AdoMet_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000001">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000003">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000004">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00086">
        <property type="entry name" value="S_AdoMet_synth1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022631">
        <property type="entry name" value="ADOMET_SYNTHASE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022630">
        <property type="entry name" value="S-AdoMet_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022629">
        <property type="entry name" value="S-AdoMet_synt_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022628">
        <property type="entry name" value="S-AdoMet_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002133">
        <property type="entry name" value="S-AdoMet_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022636">
        <property type="entry name" value="S-AdoMet_synthetase_sfam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01034">
        <property type="entry name" value="metK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11964">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02773">
        <property type="entry name" value="S-AdoMet_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02772">
        <property type="entry name" value="S-AdoMet_synt_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00438">
        <property type="entry name" value="S-AdoMet_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000497">
        <property type="entry name" value="MAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55973">
        <property type="entry name" value="S-adenosylmethionine synthetase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00376">
        <property type="entry name" value="ADOMET_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00377">
        <property type="entry name" value="ADOMET_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9 11" id="KW-0630">Potassium</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S-adenosylmethionine synthetase N-terminal" evidence="13">
        <location>
            <begin position="13"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase central" evidence="14">
        <location>
            <begin position="124"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase C-terminal" evidence="15">
        <location>
            <begin position="247"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017319904.1"/>
        </source>
    </evidence>
    <sequence length="390" mass="43209" checksum="91C5689697C47D4A" modified="2017-12-20" version="1">MDAMNDFTDDGSFMFTSESVGEGHPDKICDQISDAVLDAHLKQDPEAKVACEAVCKTGMVLLCGEITSKGSVDYQKVVRDTIKHIGYDNSDKGFDYKTCNVLVALEQQSPDIAQGVHIDRREEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNAKMAELRRDGTIPWLRPDSKTQVTVHYKQENGAMVPVRVHTVVISVQHDEFISLEEQKRILKEKVINAVVPAKYLDDKTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAKLCKRVLVQVSYAIGVNHPLSISLFTYGSSEKTEKELLHIVNKNFDLRPGVIVRDLKLKRPIYQETACYGHFGRPGFTWEVPKTLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QWQ8</accession>
    <name>A0A2D0QWQ8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein-serine O-palmitoleoyltransferase porcupine</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">porcn</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017322739.1">
        <property type="nucleotide sequence ID" value="XM_017467250.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QWQ8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003096"/>
    <dbReference type="GeneID" id="108265120"/>
    <dbReference type="KEGG" id="ipu:108265120"/>
    <dbReference type="CTD" id="64840"/>
    <dbReference type="OMA" id="WRQRSDW"/>
    <dbReference type="OrthoDB" id="5968863at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990698">
        <property type="term" value="F:palmitoleoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030258">
        <property type="term" value="P:lipid modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061355">
        <property type="term" value="P:Wnt protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049941">
        <property type="entry name" value="LPLAT_7/PORCN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906">
        <property type="entry name" value="PORCUPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906:SF22">
        <property type="entry name" value="PROTEIN-CYSTEINE N-PALMITOYLTRANSFERASE PORCUPINE ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017322739.1"/>
        </source>
    </evidence>
    <sequence length="463" mass="53057" checksum="AA0F94FA6FA91164" modified="2017-12-20" version="1">MGALSRQQFFQELPLGCLLPTAQQGLLQVWQLLIMCLACRLLWRIGRLPAYIKHLSTIACGFYTLYLFFGLHMVWVVLLSLLCYLILFLCRHSSTRGPFLSITVLIYLLLGELHMMDTNSWHKMRGSQMVVAMKAISLAFDLDKGTVENVPSPVEFMGYIYFVGTVIFGPWISFSSYREAVEGKELSVSWLVKVASSWIKSQLCLVISNCVAPYLFPYFIPVFGDKLLKNKKRRKNRGFVARLLMGYEHSMGFHFSNYFVSYLSETTTTLSGAGFTDEKDHLKWDLTMVNPLNVEFPRSVSEGVISWNLPMSQWLNIYVFKKALKFGTFQAILITYTTSTLLHGLSFHIAAVLLTLAFMTYIEYELRKRLSVILNACVLSKRCPSDCKHQNKKAFWVYFINGAFSVLAVLHLTYLSSVFGSSADDIDPDEDGMASHTFHKWSELNWTSHWLTFGLWVVYRIIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QXC3</accession>
    <name>A0A2D0QXC3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Aminopeptidase W07G4.4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">zgc:152830</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SDA103</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="7">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M17 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017323058.1">
        <property type="nucleotide sequence ID" value="XM_017467569.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QXC3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000009150"/>
    <dbReference type="GeneID" id="108265345"/>
    <dbReference type="KEGG" id="ipu:108265345"/>
    <dbReference type="OrthoDB" id="10041421at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00433">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011356">
        <property type="entry name" value="Leucine_aapep/pepB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000819">
        <property type="entry name" value="Peptidase_M17_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963:SF48">
        <property type="entry name" value="DIPEPTIDASE B, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963">
        <property type="entry name" value="LEUCINE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00883">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00481">
        <property type="entry name" value="LAMNOPPTDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00631">
        <property type="entry name" value="CYTOSOL_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytosol aminopeptidase" evidence="5">
        <location>
            <begin position="343"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017323058.1"/>
        </source>
    </evidence>
    <sequence length="513" mass="55972" checksum="CB37EC3C524D4486" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0QZY1</accession>
    <name>A0A2D0QZY1_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Small integral membrane protein 15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">smim15</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11 12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMIM15 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017323951.1">
        <property type="nucleotide sequence ID" value="XM_017468462.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017323952.1">
        <property type="nucleotide sequence ID" value="XM_017468463.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0QZY1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000011811"/>
    <dbReference type="GeneID" id="108265777"/>
    <dbReference type="KEGG" id="ipu:108265777"/>
    <dbReference type="CTD" id="643155"/>
    <dbReference type="GeneTree" id="ENSGT00940000165045"/>
    <dbReference type="OMA" id="MIDFRAW"/>
    <dbReference type="OrthoDB" id="6282848at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027877">
        <property type="entry name" value="Smim15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28644">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28644:SF1">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15086">
        <property type="entry name" value="UPF0542"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017323951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017323952.1"/>
        </source>
    </evidence>
    <sequence length="74" mass="8591" checksum="C376C3BF95B0BAD5" modified="2017-12-20" version="1">MIDVKAWAEYVVEWAAKDPYGFLTTVILALTPLFIASALLSWKLAKMIEVRDREQKKKQKRQENIAKAKRAKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R3X3</accession>
    <name>A0A2D0R3X3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7 8">Spermine oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6 7 8 9" type="primary">LOC108266460</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6 7 8" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6 7 8">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="RefSeq" id="XP_017325298.1">
        <property type="nucleotide sequence ID" value="XM_017469809.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017325299.1">
        <property type="nucleotide sequence ID" value="XM_017469810.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_047011855.1">
        <property type="nucleotide sequence ID" value="XM_047155899.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053537067.1">
        <property type="nucleotide sequence ID" value="XM_053681092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R3X3"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000013019"/>
    <dbReference type="GeneID" id="108266460"/>
    <dbReference type="KEGG" id="ipu:108266460"/>
    <dbReference type="GeneTree" id="ENSGT00940000167133"/>
    <dbReference type="OrthoDB" id="2019015at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046592">
        <property type="term" value="F:polyamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF377">
        <property type="entry name" value="SPERMINE OXIDASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="4">
        <location>
            <begin position="14"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017325298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="RefSeq" id="XP_017325299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_047011855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_053537067.1"/>
        </source>
    </evidence>
    <sequence length="485" mass="53913" checksum="A41A9E0D3FEB9E6F" modified="2017-12-20" version="1">MSRDAKIVVVGAGFAGLAAAASLVQAGFQNVKILEAKERVGGRVCTTKPFTENIIELGANWIHGQNGNPLYKLAKKHKLLAEEGLMDDSESSRDYFFKEGGAHLPDELADEVTSRFDKLISKAFDEKLPQKYRALTLGDYLDASFAESALASSEDGSRIFEWCKRNECTDEATSSLYEVSASQASEYIAPDGEFHNTLGPGGYQAILDLILKGLPSGTILSNKAVKSIEWDSQGNKDKEEKHPVRVICENDQIIQADHVIVTVSLGVLKQQARTMFEPDLPKSKLDAIERLGFGTVDKIFLCFSERFWPEDCEGIQLVWDEGPEDKAVYSSQDEEDDWEETWFKKISGFDAVARHPTVLCGWITGREALHMETLQDSEVGDICVRLLRSFTDWPVPDVSKVLVTKWGHDSNVYGSYTYIPKEVNGVREQKALATPLPPQGKASHSKPLQVLFAGEATHENFYTTTHGAYLSGVREAQRLIDHYSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R6J5</accession>
    <name>A0A2D0R6J5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14 15">Tumor necrosis factor receptor superfamily member EDAR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">edar</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14 15" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14 15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017326217.2">
        <property type="nucleotide sequence ID" value="XM_017470728.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053536635.1">
        <property type="nucleotide sequence ID" value="XM_053680660.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R6J5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000023408"/>
    <dbReference type="GeneID" id="108266902"/>
    <dbReference type="KEGG" id="ipu:108266902"/>
    <dbReference type="CTD" id="10913"/>
    <dbReference type="GeneTree" id="ENSGT00940000153259"/>
    <dbReference type="OrthoDB" id="9903718at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061972">
        <property type="term" value="P:dermal bone morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007631">
        <property type="term" value="P:feeding behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033333">
        <property type="term" value="P:fin development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046330">
        <property type="term" value="P:positive regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056762">
        <property type="entry name" value="Death_EDAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001368">
        <property type="entry name" value="TNFR/NGFR_Cys_rich_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047526">
        <property type="entry name" value="TNR19/27/EDAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12120">
        <property type="entry name" value="TNFR-CYS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12120:SF9">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER EDAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24979">
        <property type="entry name" value="Death_EDAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00208">
        <property type="entry name" value="TNFR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044573417" evidence="11">
        <location>
            <begin position="27"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="183"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="12">
        <location>
            <begin position="31"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="12">
        <location>
            <begin position="74"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="250"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="267"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017326217.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_053536635.1"/>
        </source>
    </evidence>
    <sequence length="463" mass="51070" checksum="C5F5FCA200499677" modified="2023-06-28" version="2">MARKIPRVESCLLSFLLASSVYPLRAEYSSCGENEFYNHSSSSCRPCPQCQPGQEPYMNCGYGVKDEDYDCVSCPPGKFSRGKYEICRRHKDCNALYKATVLTPGTRESDAECGACLPGYFILENRLRNIYSMMCHSCQNAPRNTKECMRTTSTSTKHVPGIPSTSTTTFPHAQKDPTGQGHLATALIIAMSTIFIMAIAIVMIIMFYILKAKTSGQACCSGQIVKTIEAPMNKQEEKKEAQENAVIFTEKDEFKPPPTKAVKSENDASSENEQLLSRSIDSDEEAAQEKQGATDLCLLSLVHLTRDKTCSNNTVSTTVNNNNNNCSNNSRTMGIHSRRKKILELYAKACGVAEGVSPVELPFDCLERTSRMLSTTYSAEKALVRTWRHLAESFGLKRDEIGGMTDGMQLFDRVSTAGYSIPDLLARLVQIERLDVVEALCFDILGAPPSGQHLTISSRCASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R6T0</accession>
    <name>A0A2D0R6T0_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Growth hormone-regulated TBC protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">grtp1b</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SDA103</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="6">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May act as a GTPase-activating protein for Rab family protein(s).</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017325530.1">
        <property type="nucleotide sequence ID" value="XM_017470041.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R6T0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000021139"/>
    <dbReference type="GeneID" id="108266563"/>
    <dbReference type="KEGG" id="ipu:108266563"/>
    <dbReference type="CTD" id="550508"/>
    <dbReference type="OMA" id="HESYEEM"/>
    <dbReference type="OrthoDB" id="294251at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000029">
        <property type="entry name" value="Growth hormone-regulated TBC protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000016">
        <property type="entry name" value="TBC1 domain family member 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF10">
        <property type="entry name" value="GROWTH HORMONE-REGULATED TBC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="4">
        <location>
            <begin position="78"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00043879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00070878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="RefSeq" id="XP_017325530.1"/>
        </source>
    </evidence>
    <sequence length="349" mass="40601" checksum="F26FC743C50A0EE3" modified="2017-12-20" version="1">MNLPEDCESTEGCISNGNSRVDKYGFEYSEEFNHESYEEMMSEYLPVLARRSEKWSRLLKENTKVDKNLKVKRYVRKGVPSKHRTLVWMAASGAQHHLQMNSGYYQSLLKVHHDPTLEETIRTDLHRTFPDNIQFHDSSQPCLLKALYNVLVAYGHHNKDVGYCQGMNFIAGYLLIITKDEEKSFWLMDALLGRILPDYYTPGMLGLKTDQDVLGELVKTKVPAVWQAMNQHNVMWCLVVSRWFICLYIDVLPMETVLRIWDCLFYEGSKILFRVALTLIRQNQNLILQAQNLPDICERFKQITHGEFVENCHAFMQNIFTEPGSLSMATITKLRETCRERIQAQDSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R905</accession>
    <name>A0A2D0R905_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">GT1 domain-containing protein isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">si:ch211-107p11.3</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017327062.1">
        <property type="nucleotide sequence ID" value="XM_017471573.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0R905"/>
    <dbReference type="GeneID" id="108267482"/>
    <dbReference type="KEGG" id="ipu:108267482"/>
    <dbReference type="OrthoDB" id="3066195at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028002">
        <property type="entry name" value="Myb_DNA-bind_5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13873">
        <property type="entry name" value="Myb_DNA-bind_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="2">
        <location>
            <begin position="5"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017327062.1"/>
        </source>
    </evidence>
    <sequence length="241" mass="26889" checksum="EB5EE04E86CE399D" modified="2017-12-20" version="1">MARERAAFFSPFEQEIILKTFEEYKAIITAKCNTAAAAKSRVEAWQKIADRLNAANPNNVKRTWQQVKIKYKNIVQCANRKRKQRFGGGLATTGFSPVEEVALWQIRGRPVIDGTILGLSSKQTGVSAGRLIATEPESSATLIQTAPTILDCAEDPIERSDSEHDEKNIVVCSHSEEGTEAYTSQEDPGPSGPSGPADGERDEDVTALYKRYLRQEIAYRQMKMKKLEKEIQLLDKQLDVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0R9T7</accession>
    <name>A0A2D0R9T7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Nuclear factor of activated T-cells, cytoplasmic 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">nfatc4</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
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                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
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                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
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                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
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                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
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                <person name="Flanagan M.E."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10">SDA103</strain>
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        <source>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
    <sequence length="480" mass="54965" checksum="12EFFEB958366946" modified="2017-12-20" version="1">MGPSSWRLCVLFCAWTWGQSMGLSRRRRAWEEKGEDGPKKCSYTFLVPEQKITGPICASRGPLLPDKDRITRIDIADVRELMSKQRREIDTLRMVVDVDGNMVSEMKLLRKESRNMNSRVTQLYMQLLHEIIRKRDNSLELAQLEGRILNATAEALRLSARYRDLEARFSTLAAVVNNQSVLIGALEERCLQVYGNHRQEPLPPPLVQVVPENIPVHVPRFSNEIQRVHSRALARERGSRAGPETTESTLELKRPLTGNFSSEGPFRDCLHAMQAGHATSGMYLLKPDESDRLMQAWCEQDVDNGGWTIIQRRKDGSVNFFRNWESYKQGFGNIDGEHWLGLENIYNLGKQGDYKLLVELQDWMEKKVYAAYSSFHLEPESESYRLRLGTYQGNAGDSLTSHNGKQFTTLDRDKDAFSGNCAHFHKGGWWYNACGQTNLNGVWYSGGVYRNKFQDGIFWADYGGGFYSMKAVRMMIRPID</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RDE9</accession>
    <name>A0A2D0RDE9_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3 4">Serine/arginine repetitive matrix protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">LOC108268026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3 4" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3 4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017328151.1">
        <property type="nucleotide sequence ID" value="XM_017472662.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017328152.1">
        <property type="nucleotide sequence ID" value="XM_017472663.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RDE9"/>
    <dbReference type="GeneID" id="108268026"/>
    <dbReference type="KEGG" id="ipu:108268026"/>
    <dbReference type="OrthoDB" id="8963010at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="117"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="144"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017328151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017328152.1"/>
        </source>
    </evidence>
    <sequence length="240" mass="26956" checksum="9B669BE09E790E8C" modified="2017-12-20" version="1">MHFLHTIEENDESEELLQEPEGGGQKESDEPSKHGIRKSRHETDSESYEATMAYSEKEPVSHGRRADGDPSTGLPSVAVKDRRKPHDEPSKSLSPSRRRNSRRSSSSTHTMSERRSSSAARSPNRRISSTLQSFRRMSSSTPGSTWRRSSSAWSSPSSSARREVLRSRSPARMSGPVVGKRDAQKTTSTKRTGEKAPSCARRRRRRTHPAPAHDDLPAPQWLIDLMFDIEEASKHELTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RDW3</accession>
    <name>A0A2D0RDW3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11">UDP glucuronosyltransferase 5 family, polypeptide G1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ugt5g1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017328732.1">
        <property type="nucleotide sequence ID" value="XM_017473243.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RDW3"/>
    <dbReference type="GeneID" id="108268336"/>
    <dbReference type="KEGG" id="ipu:108268336"/>
    <dbReference type="CTD" id="570913"/>
    <dbReference type="OrthoDB" id="5835829at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000001">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF32">
        <property type="entry name" value="UDP-GLUCURONOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="538"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017328732.1"/>
        </source>
    </evidence>
    <sequence length="571" mass="65390" checksum="EC24FEE14F10CC6E" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RIV7</accession>
    <name>A0A2D0RIV7_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nucleophosmin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">npm1b</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the nucleoplasmin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017330464.1">
        <property type="nucleotide sequence ID" value="XM_017474975.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RIV7"/>
    <dbReference type="GeneID" id="108269235"/>
    <dbReference type="KEGG" id="ipu:108269235"/>
    <dbReference type="CTD" id="553507"/>
    <dbReference type="OrthoDB" id="9946910at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010824">
        <property type="term" value="P:regulation of centrosome duplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2100:FF:000002">
        <property type="entry name" value="cell growth-regulating nucleolar protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.340:FF:000007">
        <property type="entry name" value="Nucleophosmin 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.340">
        <property type="entry name" value="Nucleoplasmin core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032569">
        <property type="entry name" value="NPM1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004301">
        <property type="entry name" value="Nucleoplasmin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024057">
        <property type="entry name" value="Nucleoplasmin_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036824">
        <property type="entry name" value="Nucleoplasmin_core_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22747:SF28">
        <property type="entry name" value="NUCLEOPHOSMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22747">
        <property type="entry name" value="NUCLEOPLASMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16276">
        <property type="entry name" value="NPM1-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03066">
        <property type="entry name" value="Nucleoplasmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69203">
        <property type="entry name" value="Nucleoplasmin-like core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Nucleoplasmin core" evidence="12">
        <location>
            <begin position="15"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleophosmin C-terminal" evidence="13">
        <location>
            <begin position="253"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="120"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="157"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017330464.1"/>
        </source>
    </evidence>
    <sequence length="303" mass="33352" checksum="968D47E66E304434" modified="2017-12-20" version="1">MAENLKDLSRPQMFLFGCVLKADKKEHKVEVDDDEVDHQLSLKAVCLGAEAEDKLHTVEIEGVTYDGKTTKIPLAVLKPSILPSLSLGGFEITPPFTFRLQSGAGPVYISGQHFVSGLGMKESDDEEEEEENNTSPVKRPSNMATGKVPLKKLKMDSDDDDDDDDEDEDDDSEDEEDDDSDEHDEDEKPVAPSPIKPSLKAPEKKKSADKQNGSPGKKDDKPGKPQSQVTIKGKDKTGAGPSGKTPSKLSISEVKNRLAAAAKEGKPFPKTDQKFENYAKHTFKISDKQVIKDLWNFVQTLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RJY6</accession>
    <name>A0A2D0RJY6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">S-adenosylmethionine-dependent nucleotide dehydratase RSAD2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Radical S-adenosyl methionine domain-containing protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">rsad2</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SDA103</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="20">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the radical SAM superfamily. RSAD2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017330837.1">
        <property type="nucleotide sequence ID" value="XM_017475348.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RJY6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000035055"/>
    <dbReference type="GeneID" id="108269452"/>
    <dbReference type="KEGG" id="ipu:108269452"/>
    <dbReference type="CTD" id="91543"/>
    <dbReference type="OMA" id="ERWFKKY"/>
    <dbReference type="OrthoDB" id="549750at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050778">
        <property type="term" value="P:positive regulation of immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026372">
        <property type="entry name" value="RSAD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051196">
        <property type="entry name" value="RSAD2/Viperin_antiviral"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04278">
        <property type="entry name" value="viperin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038283">
        <property type="entry name" value="viperin_w_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21339">
        <property type="entry name" value="RADICAL S-ADENOSYL METHIONINE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21339:SF0">
        <property type="entry name" value="S-ADENOSYLMETHIONINE-DEPENDENT NUCLEOTIDE DEHYDRATASE RSAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01088">
        <property type="entry name" value="antiviral_proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00318">
        <property type="entry name" value="Viperin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="11" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012406755" description="S-adenosylmethionine-dependent nucleotide dehydratase RSAD2" evidence="17">
        <location>
            <begin position="24"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="18">
        <location>
            <begin position="61"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00035008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00035038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00035040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00035042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="RefSeq" id="XP_017330837.1"/>
        </source>
    </evidence>
    <sequence length="353" mass="40865" checksum="3E42EABFB5323882" modified="2017-12-20" version="1">MLLSNQVALVWMLLQLCISNVHSKLSEVIRRVFSAWMGVNRGQVNSQPKRPKLSSTARGEVTTPSSVNYHFTRQCNYKCGFCFHTAKTSFVLPIDEAKRGLRLMKEAGMEKINFSGGEPFLHQKGVFLGELVQYCKQELQLPSVSIVSNGSMIRETWFQKYGEYLDILAVSCDSFDEETNQVIGRAQGRKSHIDNLYKVRNWCKEYRVAFKINSVINTFNVHEDMTEHIRGLNPVRWKVFQCLLIEGENTGKDSLREAEKFIITDQEFQDFLDRHQSINCLVPESNQKMKDSYLILDEYMRFLDCRAGRKDPSKSILDVGVEEAIKFSGFDERMFLKRGGKYVWSKAEMKLEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RKK0</accession>
    <name>A0A2D0RKK0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein si:dkey-7j14.5 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">si:dkey-7j14.5</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SDA103</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="8">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MS4A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017330676.1">
        <property type="nucleotide sequence ID" value="XM_017475187.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RKK0"/>
    <dbReference type="GeneID" id="108269355"/>
    <dbReference type="KEGG" id="ipu:108269355"/>
    <dbReference type="OrthoDB" id="8714947at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007237">
        <property type="entry name" value="CD20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030417">
        <property type="entry name" value="MS4A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320:SF128">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MEMBER 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MS4A -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04103">
        <property type="entry name" value="CD20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="RefSeq" id="XP_017330676.1"/>
        </source>
    </evidence>
    <sequence length="204" mass="22049" checksum="33C3BCE60DE14E6A" modified="2017-12-20" version="1">MSLYPSQIKAFKSSEPKTLGAVEIIIGLLTVILSTIVYKLHYHIQREVIILILNGAQLIITGIVLVHTGRRPSKCLVGTTIVLQLLTAAFDIVGFGLIARHVPFRGESYYYRDTEWCCDPLHSVWACCGEGDKAGQKEFLVNGILGTLIGFLVLVCIIGLVVALFGVYALSVSAIKEMTPIPTSTANAQYGAGGQARTNVRNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RKZ1</accession>
    <name>A0A2D0RKZ1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Protein tyrosine phosphatase type IVA 2a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ptp4a2a</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein-tyrosine phosphatase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017331182.1">
        <property type="nucleotide sequence ID" value="XM_017475693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RKZ1"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000016582"/>
    <dbReference type="GeneID" id="108269715"/>
    <dbReference type="KEGG" id="ipu:108269715"/>
    <dbReference type="CTD" id="449541"/>
    <dbReference type="OrthoDB" id="5632at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000012">
        <property type="entry name" value="protein tyrosine phosphatase type IVA 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050561">
        <property type="entry name" value="PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23339">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASE AND DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22785">
        <property type="entry name" value="Tc-R-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="14">
        <location>
            <begin position="5"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="15">
        <location>
            <begin position="79"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017331182.1"/>
        </source>
    </evidence>
    <sequence length="168" mass="19187" checksum="9C383EFCF2E96646" modified="2017-12-20" version="1">MNRPAPVEITYECMRFLITHNPTNSQLPKFTEELKTFGVQTLVRVCEATYDKAPVEKEGIEVLDWPFDDGCSPSEQIVDDWLNLLKCKFKDEPGCCIAVHCVAGLGRAPVLVAIALIECGMMYEDAVQFIRQKRRGAFNSKQLMYLEKYKPKMRLRFKDANGQNCCVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RR57</accession>
    <name>A0A2D0RR57_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="12">HHIP-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">hhipl1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SDA103</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HHIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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    <dbReference type="KEGG" id="ipu:108270352"/>
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    <dbReference type="OMA" id="YSVEVRY"/>
    <dbReference type="OrthoDB" id="10266706at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Folate_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012938">
        <property type="entry name" value="Glc/Sorbosone_DH"/>
    </dbReference>
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        <property type="entry name" value="Quinoprot_gluc/sorb_DH_b-prop"/>
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    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="HEDGEHOG-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19328:SF32">
        <property type="entry name" value="HHIP-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03024">
        <property type="entry name" value="Folate_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="Soluble quinoprotein glucose dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SRCR-like"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012971640" evidence="9">
        <location>
            <begin position="21"/>
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        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="692"/>
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        </location>
    </feature>
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        <location>
            <begin position="656"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="658"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="672"/>
            <end position="685"/>
        </location>
    </feature>
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        <location>
            <begin position="764"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010658"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017332430.1"/>
        </source>
    </evidence>
    <sequence length="802" mass="90819" checksum="BE5B6F00314FB6D1" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
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    <name>A0A2D0RRQ3_ICTPU</name>
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        <submittedName>
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    <gene>
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        <lineage>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017332851.1">
        <property type="nucleotide sequence ID" value="XM_017477362.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RRQ3"/>
    <dbReference type="GeneID" id="108270549"/>
    <dbReference type="KEGG" id="ipu:108270549"/>
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    <dbReference type="OrthoDB" id="10034447at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
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    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
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    <dbReference type="InterPro" id="IPR033593">
        <property type="entry name" value="N-RASSF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048945">
        <property type="entry name" value="RASSF8/10_RA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15286:SF13">
        <property type="entry name" value="RAS ASSOCIATION DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15286">
        <property type="entry name" value="RAS-ASSOCIATING DOMAIN CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF21712">
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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        </location>
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        <location>
            <begin position="138"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017332851.1"/>
        </source>
    </evidence>
    <sequence length="453" mass="52215" checksum="24136F1C1F7F7627" modified="2017-12-20" version="1">MEDEESKISVWVCREEKLVSGLSKRTTCADVIKVLLEEQNLHGRASASARSYCIVEKWRGFERVLPNKTKILRLWNAWGEEQENVRFVLVKQDASLASSAPRSAEARLVPSRQNPCAREGTVRQATMALSPEKQRRVVRKAFRKLERINKRRARAQCKDAERMETLVHLVVSQDHTIRQQVERIQELDHEIELYEAKVHLDRVRAHGVNYVQETYLVEGATSRHPCPSDGEQTLTQLEEYARKCEEVIRLQEDLTDRQVLLDSITRDIQEELNQRWMKRRQEDLANKDLENDSTLRVINQTWEINGCEMPPNVDVSLEDGVLLEKERIRTQLDTSLYIGLRLNTDLETVRNDLDLVQEVRKARDKELRDLLEKVDSLDLEEEEEEEEGEESCDILKDTNRVEEDTANSHGAWVEQARGLSAKACSSNDDDSDTGLSSMHSQDSDIAPVFESLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RT36</accession>
    <name>A0A2D0RT36_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC108270878</name>
    </gene>
    <organism evidence="15 16">
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        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
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        <lineage>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. eIF4A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="RefSeq" id="XP_017333330.1">
        <property type="nucleotide sequence ID" value="XM_017477841.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RT36"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000017840"/>
    <dbReference type="GeneID" id="108270878"/>
    <dbReference type="KEGG" id="ipu:108270878"/>
    <dbReference type="OMA" id="NTREQCD"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18046">
        <property type="entry name" value="DEADc_EIF4AII_EIF4AI_DDX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000089">
        <property type="entry name" value="Eukaryotic initiation factor 4A-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000031">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044728">
        <property type="entry name" value="EIF4A_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 16" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="14">
        <location>
            <begin position="37"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="68"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="250"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017333330.1"/>
        </source>
    </evidence>
    <sequence length="411" mass="46843" checksum="20A07EF20A75F320" modified="2017-12-20" version="1">MSSGSADYNSSRDRDHGGPEGMDPDGVIESNWNEITDNFDDMNLKETLLRGIYAYGFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLDVEQKETQAMVLAPTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAYHIVVGTPGRVFDMLNRKFLSPKWIKMFVLDEADEMLSRGFKDQIYEIFQKLSTNIQVVLLSATMPAEVLEVTKKFMREPVRILVKKEELTLEGIKQFYINVEREEWKLDTLCDLYETLTITQAVIFLNTRRKVDWLTEKMHGRDFTVSALHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINFVTEEDKRILRDIETFYNTTVEEMPMNVADLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RVH5</accession>
    <name>A0A2D0RVH5_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SIN3-HDAC complex associated factor, like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">sinhcafl</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017334507.3">
        <property type="nucleotide sequence ID" value="XM_017479018.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RVH5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029539"/>
    <dbReference type="GeneID" id="108271422"/>
    <dbReference type="KEGG" id="ipu:108271422"/>
    <dbReference type="CTD" id="386721"/>
    <dbReference type="OrthoDB" id="10023333at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070822">
        <property type="term" value="C:Sin3-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030336">
        <property type="term" value="P:negative regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026065">
        <property type="entry name" value="FAM60A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13422:SF13">
        <property type="entry name" value="FAMILY WITH SEQUENCE SIMILARITY 60, MEMBER A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13422">
        <property type="entry name" value="SIN3-HDAC COMPLEX-ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15396">
        <property type="entry name" value="FAM60A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017334507.3"/>
        </source>
    </evidence>
    <sequence length="226" mass="25266" checksum="37FE5D26F18FE64B" modified="2023-06-28" version="3">MFGFHKSKIYRSHEGCCICKTKSSSSRFTDSSRYEETFRLCFGLSEDRVGDICNACVLLVKRWKKLPHGSKKNWSHVVDARAGPGFKLTKPKKVKNMDGKKKSKLKKLHKFKRQNSDAHSTTSSMSPSQSPSHSNQSDDGSDVETKQRRPTPAGFSFLDLSYWKRQKVCCGIVYKGRFGEVIIDPRLFKPCCGAKQPPAVTSPAADSPCAADTLPSPLAEDLKETW</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RW93</accession>
    <name>A0A2D0RW93_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Protein inhibitor of activated STAT protein 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">pias4a</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PIAS family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="RefSeq" id="XP_017334456.1">
        <property type="nucleotide sequence ID" value="XM_017478967.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0RW93"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000001821"/>
    <dbReference type="GeneID" id="108271389"/>
    <dbReference type="KEGG" id="ipu:108271389"/>
    <dbReference type="CTD" id="406712"/>
    <dbReference type="OMA" id="TPIVECK"/>
    <dbReference type="OrthoDB" id="10263264at2759"/>
    <dbReference type="UniPathway" id="UPA00886"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 10"/>
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        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
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    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="CDD" id="cd16821">
        <property type="entry name" value="SP-RING_PIAS4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="E3 SUMO-protein ligase PIAS2 isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="E3 SUMO-protein ligase PIAS4"/>
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        <property type="entry name" value="E3 SUMO-protein ligase PIAS4"/>
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        <property type="entry name" value="PINIT domain"/>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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        <property type="entry name" value="PINIT"/>
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        <property type="entry name" value="PINIT_sf"/>
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    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
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    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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    <dbReference type="PANTHER" id="PTHR10782:SF9">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE PIAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14324">
        <property type="entry name" value="PINIT"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="24" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SAP" evidence="20">
        <location>
            <begin position="12"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="PINIT" evidence="22">
        <location>
            <begin position="106"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="SP-RING-type" evidence="21">
        <location>
            <begin position="297"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="392"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="392"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="429"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="19">
        <location>
            <begin position="442"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="462"/>
            <end position="486"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="494"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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            <dbReference type="ARBA" id="ARBA00004718"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005383"/>
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    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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            <dbReference type="ARBA" id="ARBA00022723"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
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    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50800"/>
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        <source>
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            <dbReference type="Proteomes" id="UP000221080"/>
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        <source>
            <dbReference type="RefSeq" id="XP_017334456.1"/>
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    </evidence>
    <sequence length="506" mass="55851" checksum="843F234CC14B0B5D" modified="2017-12-20" version="1">MAAELVEAMNMVKSFRVSDLQTLLASMGRSKSGLKQDLVGRALRLVQTEYSPELLKNVRQLYETRFPKAPGWLAARRPEGIPAVSYPTINSSPRNIAQGTDYLNGIPKLAAPPAAEVKLVPLPFYQNMETLLPPTELIAQNSEKLQDSQCVFELTQSQIDEIRNSSELRPGVKSVQVVLRICYTDSIGVQEDQYPPNIAVKVNQSYCHVPGYYPSNKPGVEPRRPCRPINITPWLHLSTATNRVTITWGNFGKRYSVAVYLVKVFTSAELFTKLKQCSVESAERCRERIQDKLRFDPESEITTTGLRVSLICPLVKMRLGVPCRALTCAHLQCFDAVFFLQMNEKKPTWTCPVCDKPAPFELLSIDGLLSEILKETEDVEEIEYLTDGSWRPIRDDKEKDRERENNHTPEYPVVDICVPETNGHSPAHSSTSQTGRSASGGATAGTGGASGPSGGGAVVDLTLDDSSDEEEAGGGAGDSEDTDDSQDSPAPKRGRYDYDKDLVTAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0RZY2</accession>
    <name>A0A2D0RZY2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein polybromo-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">pbrm1l</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_017480031.3"/>
    </dbReference>
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        <property type="component" value="Chromosome 11"/>
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        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:chromatin binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04717">
        <property type="entry name" value="BAH_polybromo"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05524">
        <property type="entry name" value="Bromo_polybromo_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05517">
        <property type="entry name" value="Bromo_polybromo_II"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bromo_polybromo_III"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd05518">
        <property type="entry name" value="Bromo_polybromo_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05515">
        <property type="entry name" value="Bromo_polybromo_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05526">
        <property type="entry name" value="Bromo_polybromo_VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21984">
        <property type="entry name" value="HMG-box_PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Protein polybromo-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000011">
        <property type="entry name" value="Protein polybromo-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000013">
        <property type="entry name" value="Protein polybromo-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000006">
        <property type="entry name" value="protein polybromo-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000010">
        <property type="entry name" value="protein polybromo-1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000015">
        <property type="entry name" value="protein polybromo-1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.490:FF:000002">
        <property type="entry name" value="protein polybromo-1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.490:FF:000003">
        <property type="entry name" value="protein polybromo-1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037968">
        <property type="entry name" value="PBRM1_BD5"/>
    </dbReference>
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        <property type="entry name" value="Rsc/polybromo"/>
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    <sequence length="1661" mass="189559" checksum="3E3DAEEFCBB78FA9" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
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        <name evidence="4" type="primary">ppm1j</name>
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        <name type="scientific">Ictalurus punctatus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
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    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="RefSeq"/>
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        <scope>IDENTIFICATION</scope>
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            <tissue evidence="4">Blood</tissue>
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        <property type="term" value="C:mitochondrion"/>
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        <property type="entry name" value="PPM-type phosphatase domain"/>
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        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
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        <property type="entry name" value="PROTEIN PHOSPHATASE 1H"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RefSeq" id="XP_017335946.1"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S296</accession>
    <name>A0A2D0S296_ICTPU</name>
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        <submittedName>
            <fullName evidence="11">Sterol 26-hydroxylase, mitochondrial isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">si:ch211-113j14.1</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
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        </lineage>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017336857.1">
        <property type="nucleotide sequence ID" value="XM_017481368.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0S296"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000031310"/>
    <dbReference type="GeneID" id="108272717"/>
    <dbReference type="KEGG" id="ipu:108272717"/>
    <dbReference type="OMA" id="MSWTLYH"/>
    <dbReference type="OrthoDB" id="3945418at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006700">
        <property type="term" value="P:C21-steroid hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071375">
        <property type="term" value="P:cellular response to peptide hormone stimulus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0034650">
        <property type="term" value="P:cortisol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0006704">
        <property type="term" value="P:glucocorticoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042359">
        <property type="term" value="P:vitamin D metabolic process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Cytochrome P450 302a1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050479">
        <property type="entry name" value="CYP11_CYP27_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279:SF123">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 27 SUBFAMILY A MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="468"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017336857.1"/>
        </source>
    </evidence>
    <sequence length="522" mass="59679" checksum="F0BF7B3BD52999AF" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0S9N1</accession>
    <name>A0A2D0S9N1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="11 12">PH and SEC7 domain-containing protein 1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">LOC108274093</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11 12">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Ruffle membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017339444.1">
        <property type="nucleotide sequence ID" value="XM_017483955.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017339445.1">
        <property type="nucleotide sequence ID" value="XM_017483956.3"/>
    </dbReference>
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    <dbReference type="GeneID" id="108274093"/>
    <dbReference type="KEGG" id="ipu:108274093"/>
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    <dbReference type="OrthoDB" id="2157641at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13295">
        <property type="entry name" value="PH_EFA6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000004">
        <property type="entry name" value="PH and SEC7 domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000054">
        <property type="entry name" value="PH and SEC7 domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041681">
        <property type="entry name" value="PH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001605">
        <property type="entry name" value="PH_dom-spectrin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF334">
        <property type="entry name" value="PH AND SEC7 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15410">
        <property type="entry name" value="PH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00683">
        <property type="entry name" value="SPECTRINPH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="9">
        <location>
            <begin position="1020"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="1228"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="181"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="596"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="782"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="959"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1473"/>
            <end position="1501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="19"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="197"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="607"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="630"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="784"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="959"/>
            <end position="978"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017339444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="RefSeq" id="XP_017339445.1"/>
        </source>
    </evidence>
    <sequence length="1501" mass="165864" checksum="EB1B5D128D97BD89" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SCY1</accession>
    <name>A0A2D0SCY1_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4 5">von Willebrand factor A domain-containing protein 5A isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">LOC108274632</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4 5">Blood</tissue>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="1">
        <location>
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            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_053541499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_053541500.1"/>
        </source>
    </evidence>
    <sequence length="792" mass="86817" checksum="06D617638D7D909B" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SFP3</accession>
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        <submittedName>
            <fullName evidence="10 11">Caprin-2 isoform X1</fullName>
        </submittedName>
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        <name evidence="10 11" type="primary">caprin2</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
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        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SDA103</strain>
        </source>
    </reference>
    <reference evidence="10 11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="10 11">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the caprin family.</text>
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        <property type="nucleotide sequence ID" value="XM_017486058.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053541980.1">
        <property type="nucleotide sequence ID" value="XM_053686005.1"/>
    </dbReference>
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    <dbReference type="STRING" id="7998.ENSIPUP00000024433"/>
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    <dbReference type="KEGG" id="ipu:108275312"/>
    <dbReference type="CTD" id="65981"/>
    <dbReference type="OMA" id="NHNQHGE"/>
    <dbReference type="OrthoDB" id="10062814at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090263">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
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    <dbReference type="InterPro" id="IPR028816">
        <property type="entry name" value="Caprin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022070">
        <property type="entry name" value="Caprin-1_C"/>
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    <dbReference type="InterPro" id="IPR041637">
        <property type="entry name" value="Caprin-1_dimer"/>
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    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22922:SF5">
        <property type="entry name" value="CAPRIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22922">
        <property type="entry name" value="GPI-ANCHORED PROTEIN P137"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12287">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18293">
        <property type="entry name" value="Caprin-1_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
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        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0652">Protein synthesis inhibitor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
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        <location>
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            <end position="950"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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            <begin position="1"/>
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    </feature>
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    </feature>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023193"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017341547.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053541980.1"/>
        </source>
    </evidence>
    <sequence length="950" mass="103374" checksum="0AA16D4BA727E3D2" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SGB8</accession>
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        <recommendedName>
            <fullName evidence="10">USP6 N-terminal-like protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="15" type="primary">si:dkeyp-19e1.3</name>
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    <organism evidence="14 15">
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        <name type="common">Channel catfish</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
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        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
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            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SDA103</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="15">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Acts as a GTPase-activating protein for RAB5A and RAB43. Involved in receptor trafficking. In complex with EPS8 inhibits internalization of EGFR. Involved in retrograde transport from the endocytic pathway to the Golgi apparatus. Involved in the transport of Shiga toxin from early and recycling endosomes to the trans-Golgi network. Required for structural integrity of the Golgi complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with EPS8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017341305.1">
        <property type="nucleotide sequence ID" value="XM_017485816.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SGB8"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000008023"/>
    <dbReference type="GeneID" id="108275185"/>
    <dbReference type="KEGG" id="ipu:108275185"/>
    <dbReference type="OrthoDB" id="294251at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000010">
        <property type="entry name" value="Putative USP6 N-terminal-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.750:FF:000001">
        <property type="entry name" value="TBC1 domain family member 10A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000019">
        <property type="entry name" value="USP6 N-terminal like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF25">
        <property type="entry name" value="RAB-GAP TBC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="13">
        <location>
            <begin position="99"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="380"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="568"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="112"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="449"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="571"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="RefSeq" id="XP_017341305.1"/>
        </source>
    </evidence>
    <sequence length="722" mass="82198" checksum="EF5F0A3E1252838F" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SI26</accession>
    <name>A0A2D0SI26_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma</fullName>
            <ecNumber evidence="8">3.1.4.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC108275843</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SDA103</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="11">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Participates in processes of transmission and amplification of the visual signal. cGMP-PDEs are the effector molecules in G-protein-mediated phototransduction in vertebrate rods and cones.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>3',5'-cyclic GMP + H2O = GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16957"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="EC" id="3.1.4.35"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the rod/cone cGMP-PDE gamma subunit family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.35" evidence="8"/>
    <dbReference type="RefSeq" id="XP_017342362.1">
        <property type="nucleotide sequence ID" value="XM_017486873.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SI26"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000014545"/>
    <dbReference type="GeneID" id="108275843"/>
    <dbReference type="KEGG" id="ipu:108275843"/>
    <dbReference type="OrthoDB" id="8525078at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042622">
        <property type="term" value="C:photoreceptor outer segment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047555">
        <property type="term" value="F:3',5'-cyclic-GMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045742">
        <property type="term" value="P:positive regulation of epidermal growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045745">
        <property type="term" value="P:positive regulation of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1120.10:FF:000001">
        <property type="entry name" value="retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1120.10">
        <property type="entry name" value="Retinal cGMP phosphodiesterase, gamma subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006952">
        <property type="entry name" value="PDE6_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037030">
        <property type="entry name" value="PDE6_gamma_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12122">
        <property type="entry name" value="RETINAL CONE RHODOPSIN-SENSITIVE CGMP 3',5'-CYCLIC PHOSPHODIESTERASE GAMMA-SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12122:SF10">
        <property type="entry name" value="RHODOPSIN-SENSITIVE CGMP 3',5'-CYCLIC PHOSPHODIESTERASE SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04868">
        <property type="entry name" value="PDE6_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000969">
        <property type="entry name" value="35-cGMP_Pdiase_g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0140">cGMP</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="6" id="KW-0844">Vision</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_017342362.1"/>
        </source>
    </evidence>
    <sequence length="99" mass="10866" checksum="DE67A50CC2CBE6E4" modified="2017-12-20" version="1">MSLQTEPAFMNVNMSKSEHKAGSKMATRATGPGSPHKGPPRFKQRSTRQFKSKPPKRGVIGFGDEIPGMEGLGTDITVICPWEAFSHLELHELAQYGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SKK5</accession>
    <name>A0A2D0SKK5_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mimecan</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Osteoglycin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ognb</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SDA103</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="17">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Induces bone formation in conjunction with TGF-beta-1 or TGF-beta-2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class III subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017343021.1">
        <property type="nucleotide sequence ID" value="XM_017487532.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SKK5"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000020941"/>
    <dbReference type="GeneID" id="108276132"/>
    <dbReference type="KEGG" id="ipu:108276132"/>
    <dbReference type="CTD" id="100170815"/>
    <dbReference type="OMA" id="YIRTNMY"/>
    <dbReference type="OrthoDB" id="7451790at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061975">
        <property type="term" value="P:articular cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060348">
        <property type="term" value="P:bone development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043547">
        <property type="entry name" value="Mimecan/Epiphycan/Opticin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46269">
        <property type="entry name" value="EPIPHYCAN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46269:SF1">
        <property type="entry name" value="MIMECAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0339">Growth factor</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012994296" description="Mimecan" evidence="15">
        <location>
            <begin position="22"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="RefSeq" id="XP_017343021.1"/>
        </source>
    </evidence>
    <sequence length="292" mass="33339" checksum="C4ABE2C45AE6F15E" modified="2017-12-20" version="1">MDLRMLFFSFLLVPWMLYATAQNDQLELQNGGFLRVDLDEWQSDDSFFMEVKKKKEKVIPAPDYESVDNANDEGSAVEKKEADLPTCLLCVCLSGSVYCEEVSPEMTTVPPFPKETTYLYARFNKITRITNKDFADVGTLKRIDLTGNLISEIEDGAFSKLDQLEELTLAENKLIKLPMLPPKLILLNANHNQLRTKGIRSTVLKKLRKLAYLYLGDNELEAIPPLPESLHVVHLHNNNITTLTDETFCKGNDTYYIRYNMQEVRLDGNPLMLAQHPNSFICLRALPIGLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SRT2</accession>
    <name>A0A2D0SRT2_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Eukaryotic peptide chain release factor subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">etf1b</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic release factor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017345015.1">
        <property type="nucleotide sequence ID" value="XM_017489526.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SRT2"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000029921"/>
    <dbReference type="GeneID" id="108277127"/>
    <dbReference type="KEGG" id="ipu:108277127"/>
    <dbReference type="CTD" id="266989"/>
    <dbReference type="OMA" id="GPGTEKM"/>
    <dbReference type="OrthoDB" id="10254527at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000009">
        <property type="entry name" value="Eukaryotic peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000001">
        <property type="entry name" value="Eukaryotic peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.960.10:FF:000001">
        <property type="entry name" value="Eukaryotic peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.960.10">
        <property type="entry name" value="eRF1 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024049">
        <property type="entry name" value="eRF1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004403">
        <property type="entry name" value="Peptide_chain-rel_eRF1/aRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03676">
        <property type="entry name" value="aRF1_eRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10113">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03463">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55481">
        <property type="entry name" value="N-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="7">
        <location>
            <begin position="6"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017345015.1"/>
        </source>
    </evidence>
    <sequence length="443" mass="49610" checksum="A0F0650E276C00B9" modified="2017-12-20" version="1">MADDPNAADRNVEIWKIKKLIKSLEAARGNGTSMISLIIPPKDQISRVAKMLADEFGTASNIKSRVNRLSVLGAITSVQQRLKLYNKVPPNGLVVYCGTIMTEEGKEKKVNIDFEPFKPINTSLYLCDNKFHTEALTALLSDDSKFGFIVIDGSGALFGTLQGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKVAETAVQLFVSNDKVNVAGMVLAGSADFKTELSQSDMFDPRLQAKVLKLVDISYGGENGFNQAIELSAEVLSNVKFIQEKKLIGHYFDEISQDTGKYCFGVDDTLKALEMGAMEILIVYENLDTMRYVLRCHGAEGGGPENDEKTLYLTPVQEKDKSHFTDKETGQEHELLESMPLLEWFANNYKKFGATLEIVTDKSQEGSQFVKGFGGIGGILRYRVDFQGMDYQVEDEELYNLDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SU23</accession>
    <name>A0A2D0SU23_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Testis-expressed protein 12 isoform X2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC108277785</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SDA103</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="2">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017346181.1">
        <property type="nucleotide sequence ID" value="XM_017490692.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SU23"/>
    <dbReference type="GeneID" id="108277785"/>
    <dbReference type="KEGG" id="ipu:108277785"/>
    <dbReference type="OrthoDB" id="6155282at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029193">
        <property type="entry name" value="TEX12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37349">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37349:SF1">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15219">
        <property type="entry name" value="TEX12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="RefSeq" id="XP_017346181.1"/>
        </source>
    </evidence>
    <sequence length="157" mass="17235" checksum="D3E5F74F4308972E" modified="2017-12-20" version="1">MAAHLTDPTDFRQGQPVACLPGCVQCSHCRTMTGKGSQMTLRGTDVKGTKLKMPEAEHAGAFCDDSPAKRMKVSRTKLAPVDGTEGFEKAWSGAVREINCLFTKYPEMLRERAAVDASQIQELTNILTEALSLESQLKEKKEHLRQSLAVISDKLQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SUE3</accession>
    <name>A0A2D0SUE3_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Olfactory receptor 52K1-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC108277637</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SDA103</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="14">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017345930.1">
        <property type="nucleotide sequence ID" value="XM_017490441.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SUE3"/>
    <dbReference type="GeneID" id="108277637"/>
    <dbReference type="KEGG" id="ipu:108277637"/>
    <dbReference type="OMA" id="MFICATA"/>
    <dbReference type="OrthoDB" id="5969463at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000013">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF391">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="63"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="204"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="42"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="RefSeq" id="XP_017345930.1"/>
        </source>
    </evidence>
    <sequence length="314" mass="35963" checksum="1134D656C3BC0AF2" modified="2017-12-20" version="1">MLDPVQNFSFTTFTFTGFHDLGEWQPILSIPYLLMFLLSSTSNLTLIYLIITQRALHSPMCILIGLMAVVDLSMPIFCVPNMLLSFLFNWKGISLEGCLVQMFCISLVGTFHSTILLWMALDRFFAICRPLYYHKYMGMPNFLKFIIFPVIRNLFFITTMVSWAGKLTFCETNEIDHCFCEHMALVQLACGDISINNALGLLTVFLTITADFIFITISYIAILSSILRSGKACLKAVNTCITHIIVMTVSLTFALIAFMSYRIRNNFSPSSRVFLSTMYMFFPSCFNPIIYGVRTKEIREQFLKLVKYVKVFPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SUQ2</accession>
    <name>A0A2D0SUQ2_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein LOC108277887 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC108277887</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SDA103</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="5">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017346408.1">
        <property type="nucleotide sequence ID" value="XM_017490919.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SUQ2"/>
    <dbReference type="GeneID" id="108277887"/>
    <dbReference type="KEGG" id="ipu:108277887"/>
    <dbReference type="OrthoDB" id="10012075at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012361576" evidence="3">
        <location>
            <begin position="20"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="RefSeq" id="XP_017346408.1"/>
        </source>
    </evidence>
    <sequence length="302" mass="33189" checksum="B62C3736D0F76297" modified="2017-12-20" version="1">MNILIIFVLIFTFTLKANGADFDNAAATKNVTGNFSNQDGNMTSNPTFTSTTEMHVAVNTPSISNLTNPSTIPSESENKADRDGKMLNNCNETNKQLEQKVEGSFKLNLVMILVFILGVICSTIIFSTVLCFVIICDRCRKTDANTDKEGITLEGVRSGHDQLMMQSDEDQADEQAPLQVQSPVEVSTNTSEINSGTKEEMKISETEQTEGAGEVNEVDYASINYSLLQKKDNGGLKPQKVESDYAEIQLKKMSEGEEVETLQDGEKQDDVKKHEVNQDGGNQGLDGVTESQKQVELQKVQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SVE9</accession>
    <name>A0A2D0SVE9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zona pellucida sperm-binding protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Zona pellucida glycoprotein 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Zona pellucida protein B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC108278048</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">SDA103</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="22">Blood</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP4 may act as a sperm receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Zona pellucida</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017346662.1">
        <property type="nucleotide sequence ID" value="XM_017491173.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SVE9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000014666"/>
    <dbReference type="GeneID" id="108278048"/>
    <dbReference type="KEGG" id="ipu:108278048"/>
    <dbReference type="OrthoDB" id="8919081at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035805">
        <property type="term" value="C:egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032190">
        <property type="term" value="F:acrosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035804">
        <property type="term" value="F:structural constituent of egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060468">
        <property type="term" value="P:prevention of polyspermy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00111">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.110.10">
        <property type="entry name" value="Spasmolytic Protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000519">
        <property type="entry name" value="P_trefoil_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044913">
        <property type="entry name" value="P_trefoil_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051148">
        <property type="entry name" value="Zona_Pellucida_Domain_gp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055356">
        <property type="entry name" value="ZP-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23343">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23343:SF31">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00088">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23344">
        <property type="entry name" value="ZP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00018">
        <property type="entry name" value="PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57492">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51448">
        <property type="entry name" value="P_TREFOIL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="9 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="11" id="KW-0278">Fertilization</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012858751" description="Zona pellucida sperm-binding protein 4" evidence="18">
        <location>
            <begin position="23"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="P-type" evidence="20">
        <location>
            <begin position="54"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="19">
        <location>
            <begin position="97"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="66"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="RefSeq" id="XP_017346662.1"/>
        </source>
    </evidence>
    <sequence length="407" mass="44025" checksum="BCE7F28B091A7E2E" modified="2017-12-20" version="1">MANTRVSLALVGLFLVFNYVSSTPASLQAVKMPPQAPQLPGKPTLQPQMSGQVAKCQVQDYERVPCGEPGINSDQCTIINCCFDGQQCYYGKLVTVQCTLDGQFVLVVARDATVPRISLESISMLGGNTGPCSPVDSNTAFAIYQFPVTACGTTMMVQGGYVVYENRMLSSYEVGVGPRGAITRDTHYELYFQCKYSGVSLATLAIDPSANADPPSIVASGPLQVELRLGNGHCVAKGCVEDEVAYTSYYSAADYPVTKVLREPVYVEVHIVGRTDPNIVLVLGGCWATASPNPYSLPQWDLLVNGCPYKDDRYLTKLMPVNGMSGLAYPTHYRRFVLKMFTFVDPDSMAPMHETLYIHCSTAVCLPTAQDSCEPTCARRRRDVAADGSSPSTSGIVSSGEVIFTQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0SXB6</accession>
    <name>A0A2D0SXB6_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">pitx1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SDA103</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="16">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10 11 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the paired homeobox family. Bicoid subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017347322.1">
        <property type="nucleotide sequence ID" value="XM_017491833.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0SXB6"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000028392"/>
    <dbReference type="GeneID" id="108278446"/>
    <dbReference type="KEGG" id="ipu:108278446"/>
    <dbReference type="CTD" id="5307"/>
    <dbReference type="OMA" id="CKNGYLP"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043010">
        <property type="term" value="P:camera-type eye development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021536">
        <property type="term" value="P:diencephalon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000031">
        <property type="entry name" value="Homeobox protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016233">
        <property type="entry name" value="Homeobox_Pitx/unc30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45882:SF1">
        <property type="entry name" value="PITUITARY HOMEOBOX 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45882">
        <property type="entry name" value="PITUITARY HOMEOBOX HOMOLOG PTX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000563">
        <property type="entry name" value="Homeobox_protein_Pitx/Unc30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="3 10" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 11 12" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="83"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="85"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="47"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="RefSeq" id="XP_017347322.1"/>
        </source>
    </evidence>
    <sequence length="291" mass="32354" checksum="5ABCBEAE82852E20" modified="2017-12-20" version="1">MELNLSKSVMSCGAAGGMNLDRIATDAARLPPARDMASAFHAHVARDTLEHTSSESSDTETAEKERTVEQRTEDGNPDDPKKKKQRRQRTHFTSQQLQELEATFQRNRYPDMSTREEIAVWTNLTEARVRVWFKNRRAKWRKRERNQQMDLCKNGYLPQFGGLVQPYEDMYPAYGYNNWTNKGLAPKNFPFFNSMSPLTSQPVLSSPSSISSMSMSMSMASSMNSISNLNGLGGSSINSAMSSSACPYGAPPPAPYGVYRDTCSSGLATLRLKPKQHPGFGYGGSACQYNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T082</accession>
    <name>A0A2D0T082_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein ABHD15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC108278934</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017348191.2">
        <property type="nucleotide sequence ID" value="XM_017492702.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T082"/>
    <dbReference type="ESTHER" id="ictpu-a0a2d0t082">
        <property type="family name" value="abh_upf0017"/>
    </dbReference>
    <dbReference type="GeneID" id="108278934"/>
    <dbReference type="KEGG" id="ipu:108278934"/>
    <dbReference type="OrthoDB" id="247542at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034338">
        <property type="term" value="F:short-chain carboxylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050960">
        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF96">
        <property type="entry name" value="PROTEIN ABHD15-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5039907930" evidence="2">
        <location>
            <begin position="19"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017348191.2"/>
        </source>
    </evidence>
    <sequence length="344" mass="39243" checksum="F739017032CF03AE" modified="2023-06-28" version="2">MWEFFFCLMPSMFLFTIARLFQSKWIYHLVEEGTRQLTLHIWIIICSILKLPHPSVLYGTGDKESKIKLIYKSIALANNIIQNCKALACAPPARWPKADPHLQMFFNFIWPIEDDAQEHCGITFTRDHLLIQDGGIVALDWAVRLKDLNAQAKREHYLGERAPGYHTCTPSIIILIPNALGKITQNLLSLCRLALQQGFYTVVFHRRGHGRCLLTTPRYQEFGDPSDLVQAVLYIKSRHPSSDLFAINEGSGSGLMLSYLGERGSSSHLVAAACISPVFYRQLFFETPCPKLYNEASLFYRKLQMSRYATALSSVMDTEKIFSCRSLQDMEKLMFCSVRLLDTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T0U9</accession>
    <name>A0A2D0T0U9_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Nuclear receptor subfamily 2 group E member 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Nuclear receptor TLX</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Protein tailless homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">nr2e1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017348544.1">
        <property type="nucleotide sequence ID" value="XM_017493055.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T0U9"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000003850"/>
    <dbReference type="GeneID" id="108279087"/>
    <dbReference type="KEGG" id="ipu:108279087"/>
    <dbReference type="CTD" id="7101"/>
    <dbReference type="OrthoDB" id="10045640at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07163">
        <property type="entry name" value="NR_DBD_TLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06950">
        <property type="entry name" value="NR_LBD_Tlx_PNR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000019">
        <property type="entry name" value="Nuclear receptor subfamily 2 group E member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000019">
        <property type="entry name" value="Nuclear receptor subfamily 2 group E member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050274">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24083">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10 15 19" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 15" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="16">
        <location>
            <begin position="24"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="17">
        <location>
            <begin position="156"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00041813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017348544.1"/>
        </source>
    </evidence>
    <sequence length="396" mass="44021" checksum="5612E667AA6FA733" modified="2017-12-20" version="1">MSKAASSTSGCLDILNVKSSRILDIPCKVCGDRSSGKHYGVYACDGCSGFFKRSIRRNRTYVCKSGTQGGCPVDKTHRNQCRACRLKKCLEVNMNKDAVQHERGPRTSTIRKQVALYFRGHKEVNGSSSHFPNTSIPGLHFFTTVTQLETHSLELNTVPSTPERQGMVGLAQPTPKYPHEVSGTPMYLYEVATESVCESAARLLFMSIKWAKSVPAFSTLPLQDQLILLEDAWRELFVLGLAQWSIPVDSNTLLAVSGMTTENTESQRLNKVVSEIQALQEVVTRFRQLRLDATEFACLKCIVTFKAVPTHSGAELRSFRNASAIAALQDEAQLTLNSYIHTRYPTQPCRFGKLLLLLPALRSVSASTIEEVFFRKTIGNVPITRLLSDMYKSSDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T1W7</accession>
    <name>A0A2D0T1W7_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="9 10 11">Myogenesis-regulating glycosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9 10 11" type="primary">myorg</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SDA103</strain>
        </source>
    </reference>
    <reference evidence="9 10 11" key="2">
        <citation type="submission" date="2025-04" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9 10 11">Blood</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017348918.1">
        <property type="nucleotide sequence ID" value="XM_017493429.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_017348920.1">
        <property type="nucleotide sequence ID" value="XM_017493431.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_053543843.1">
        <property type="nucleotide sequence ID" value="XM_053687868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T1W7"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000015889"/>
    <dbReference type="GeneID" id="108279294"/>
    <dbReference type="KEGG" id="ipu:108279294"/>
    <dbReference type="CTD" id="57462"/>
    <dbReference type="GeneTree" id="ENSGT00940000161008"/>
    <dbReference type="OMA" id="CDRTTDR"/>
    <dbReference type="OrthoDB" id="10070917at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048741">
        <property type="term" value="P:skeletal muscle fiber development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06592">
        <property type="entry name" value="GH31_NET37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000037">
        <property type="entry name" value="Putative family 31 glucosidase KIAA1161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050985">
        <property type="entry name" value="Alpha-glycosidase_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43053">
        <property type="entry name" value="GLYCOSIDASE FAMILY 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43053:SF4">
        <property type="entry name" value="MYOGENESIS-REGULATING GLYCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="6">
        <location>
            <begin position="294"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="7">
        <location>
            <begin position="600"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="RefSeq" id="XP_017348918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="RefSeq" id="XP_017348920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="RefSeq" id="XP_053543843.1"/>
        </source>
    </evidence>
    <sequence length="685" mass="78353" checksum="C03527B5D952BEB5" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T2L8</accession>
    <name>A0A2D0T2L8_ICTPU</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Solute carrier organic anion transporter family member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">slco1c1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">SDA103</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="19">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>3,3',5'-triiodo-L-thyronine(out) = 3,3',5'-triiodo-L-thyronine(in)</text>
            <dbReference type="Rhea" id="RHEA:71815"/>
            <dbReference type="ChEBI" id="CHEBI:57261"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-thyroxine sulfate(out) = L-thyroxine sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:73311"/>
            <dbReference type="ChEBI" id="CHEBI:176512"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-thyroxine(out) = L-thyroxine(in)</text>
            <dbReference type="Rhea" id="RHEA:71819"/>
            <dbReference type="ChEBI" id="CHEBI:58448"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cell membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the organo anion transporter (TC 2.A.60) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_017349163.1">
        <property type="nucleotide sequence ID" value="XM_017493674.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T2L8"/>
    <dbReference type="GeneID" id="108279430"/>
    <dbReference type="KEGG" id="ipu:108279430"/>
    <dbReference type="CTD" id="53919"/>
    <dbReference type="OrthoDB" id="5062115at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015347">
        <property type="term" value="F:sodium-independent organic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:sodium-independent organic anion transport"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Solute carrier organic anion transporter family member"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Kazal_dom"/>
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        <property type="entry name" value="Kazal_dom_sf"/>
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        <property type="entry name" value="MFS_trans_sf"/>
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        <property type="entry name" value="OATP"/>
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        <property type="entry name" value="oat"/>
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        <property type="entry name" value="ORGANIC ANION TRANSPORTER"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SOLUTE CARRIER ORGANIC ANION TRANSPORTER FAMILY MEMBER 1C1"/>
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    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03137">
        <property type="entry name" value="OATP"/>
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        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="8 15" id="KW-0406">Ion transport</keyword>
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            <end position="59"/>
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    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="15">
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    <feature type="transmembrane region" description="Helical" evidence="15">
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            <begin position="220"/>
            <end position="241"/>
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    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="271"/>
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    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="354"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="392"/>
            <end position="412"/>
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    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="544"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="16">
        <location>
            <begin position="45"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="17">
        <location>
            <begin position="469"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00050960"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00051340"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00052624"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU362056"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="RefSeq" id="XP_017349163.1"/>
        </source>
    </evidence>
    <sequence length="594" mass="65338" checksum="8B316F636597EFD0" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T4U4</accession>
    <name>A0A2D0T4U4_ICTPU</name>
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        <submittedName>
            <fullName evidence="4">Periphilin-1 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pphln1</name>
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    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
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            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017349836.2">
        <property type="nucleotide sequence ID" value="XM_017494347.3"/>
    </dbReference>
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    <dbReference type="STRING" id="7998.ENSIPUP00000022965"/>
    <dbReference type="GeneID" id="108279830"/>
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    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045814">
        <property type="term" value="P:negative regulation of gene expression, epigenetic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097355">
        <property type="term" value="P:protein localization to heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22896">
        <property type="entry name" value="periphilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057603">
        <property type="entry name" value="Periphilin-1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028851">
        <property type="entry name" value="Pphln1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15836">
        <property type="entry name" value="PERIPHILIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15836:SF4">
        <property type="entry name" value="PERIPHILIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25234">
        <property type="entry name" value="Periphilin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Periphilin-1 C-terminal" evidence="2">
        <location>
            <begin position="297"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="291"/>
        </location>
    </feature>
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            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
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            <begin position="126"/>
            <end position="151"/>
        </location>
    </feature>
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            <end position="202"/>
        </location>
    </feature>
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        <location>
            <begin position="221"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_017349836.2"/>
        </source>
    </evidence>
    <sequence length="387" mass="43562" checksum="B9F808457BD84278" modified="2023-06-28" version="2">MVTSKQVNISKEAARAGHRSSSSSLSYDCTREHHFLGKAMAFRRDRSIRDMYEQRFHQDRAAPYPRGAGGERRAFGRPDEDYTRNFDYDSPRFYPNGGPRNYHNEDKKGYQSDSYFSNESRGGPANRRDDYPYYRGPKDDAHSGRQGDFRGRSRVVSSPNARVQGSYPAPRSLSAAGPNPGDDTLIQAIMNLDRGEEREGLRRKAPFPPVHDCSPTRQDVSPSPHSRSGSSISSRSYSPDRGKSHTYQPPLGKNRDRAPGPAVSVSRDGSPHSSVSASKEEMPVIERQTDEPLAPADEATTIADDFHERRAQAIAAKAREIEKVYRQDCETFGMVVKMLVAKDPSLEKHLQNPLKENLGEIRERCLEDLRHFISELDEVVRQPEPTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T6Q0</accession>
    <name>A0A2D0T6Q0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="24">Potassium voltage-gated channel subfamily KQT member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">kcnq3</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">SDA103</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="24">Blood</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Cs(+)(in) = Cs(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78555"/>
            <dbReference type="ChEBI" id="CHEBI:49547"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Rb(+)(in) = Rb(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78547"/>
            <dbReference type="ChEBI" id="CHEBI:49847"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_017350571.1">
        <property type="nucleotide sequence ID" value="XM_017495082.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T6Q0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000019586"/>
    <dbReference type="GeneID" id="108280236"/>
    <dbReference type="KEGG" id="ipu:108280236"/>
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    <dbReference type="OrthoDB" id="8879391at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
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    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="potassium voltage-gated channel subfamily KQT member 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Putative potassium voltage-gated channel subfamily KQT member 2"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR020969">
        <property type="entry name" value="Ankyrin-G_BS"/>
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        <property type="entry name" value="Ion_trans_dom"/>
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        <property type="entry name" value="K_chnl_volt-dep_KCNQ"/>
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        <property type="entry name" value="K_chnl_volt-dep_KCNQ3"/>
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        <property type="entry name" value="K_chnl_volt-dep_KCNQ_C"/>
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        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47735">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 4"/>
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    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
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    </dbReference>
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        <property type="entry name" value="KCNQ_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11956">
        <property type="entry name" value="KCNQC3-Ank-G_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
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    <dbReference type="PRINTS" id="PR01462">
        <property type="entry name" value="KCNQ3CHANNEL"/>
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        <property type="entry name" value="KCNQCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0630">Potassium</keyword>
    <keyword evidence="7" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="129"/>
            <end position="151"/>
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    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="172"/>
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    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="235"/>
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    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="310"/>
            <end position="336"/>
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    <feature type="domain" description="Ion transport" evidence="21">
        <location>
            <begin position="99"/>
            <end position="336"/>
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    </feature>
    <feature type="domain" description="Potassium channel voltage dependent KCNQ C-terminal" evidence="22">
        <location>
            <begin position="440"/>
            <end position="635"/>
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        <location>
            <begin position="1"/>
            <end position="23"/>
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        <location>
            <begin position="420"/>
            <end position="455"/>
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        <location>
            <begin position="566"/>
            <end position="588"/>
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    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="771"/>
            <end position="795"/>
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        <location>
            <begin position="14"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="446"/>
            <end position="455"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="777"/>
            <end position="791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
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    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
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            <dbReference type="ARBA" id="ARBA00022882"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00044657"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00044691"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="Pfam" id="PF00520"/>
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        <source>
            <dbReference type="Pfam" id="PF03520"/>
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        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
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    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="RefSeq" id="XP_017350571.1"/>
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    </evidence>
    <sequence length="804" mass="90393" checksum="75C4E640C5686EAC" modified="2017-12-20" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T7K6</accession>
    <name>A0A2D0T7K6_ICTPU</name>
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        <submittedName>
            <fullName evidence="9">Tripartite motif-containing protein 16 isoform X1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC108280424</name>
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    <organism evidence="8 9">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">SDA103</strain>
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    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="9">Blood</tissue>
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        <property type="component" value="Chromosome 20"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SPRY_PRY_SNTX"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
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        <property type="entry name" value="B30.2/SPRY_sf"/>
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    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
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    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
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    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
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        <property type="entry name" value="Znf_B-box"/>
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        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF5">
        <property type="entry name" value="E3 UBIQUITIN_ISG15 LIGASE TRIM25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BUTYPHLNCDUF"/>
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        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00449">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="6">
        <location>
            <begin position="69"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="7">
        <location>
            <begin position="282"/>
            <end position="476"/>
        </location>
    </feature>
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        <location>
            <begin position="182"/>
            <end position="219"/>
        </location>
    </feature>
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    </evidence>
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    </evidence>
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    </evidence>
    <sequence length="478" mass="55246" checksum="12F90543314C61FF" modified="2017-12-20" version="1">MSGFLDTMARCKNYTTFGYVECGSCTGKKQKAIKSCLVCLDSYCPSHFELHEELHSGQRHKIMNATGFLQDKICSRHDNLLEVFCCLDQQCICLMCMMDDHKGHRIVSASAERTEKQSQMGESKMKSQQRILERKRELGEMQKAVKSHKQSAQAALEESEKTFTEIICFIEKKRSEVQDLIRAQEKIAVSRAEGLLKQLEEEIAELQRQDEELQQLSLTEDHIHFLQRYVFLTATLGSSDLPTITVSSVLSFEEVAKSVTELKKHLEDVCKADMEKIEKRVKQIQILTPMTREEVLQYYCELTMDPNTAYRRLSLSDSNRAVSNYDRDLPYPDHPERFDWPQVLCEQPVCGRFYWEVEWSGRNGVNIAVSYKSIHRKGGHNECWFGCSDQSWRLYCSPFRYAFRHHGKETYIPKMDNSTRIGVYVDYNAGTLSFYSIAGKMILLYIVQCEFTEPLYPGFTVWPGSKLTLCCPTKVKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2017-12-20" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A2D0T8D0</accession>
    <name>A0A2D0T8D0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coiled-coil-helix-coiled-coil-helix domain-containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">chchd2</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_017351138.1">
        <property type="nucleotide sequence ID" value="XM_017495649.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A2D0T8D0"/>
    <dbReference type="STRING" id="7998.ENSIPUP00000011625"/>
    <dbReference type="GeneID" id="108280552"/>
    <dbReference type="KEGG" id="ipu:108280552"/>
    <dbReference type="CTD" id="51142"/>
    <dbReference type="OrthoDB" id="1106148at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055304">
        <property type="entry name" value="CHCHD2/10-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 2/NUR77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523:SF3">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN-CONTAINING PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_017351138.1"/>
        </source>
    </evidence>
    <sequence length="164" mass="17247" checksum="913ACE0C24CCD2E3" modified="2017-12-20" version="1">MPRGSRSRTSRMAPPSYSSRPAPPPPVARVAPPPAPAHAPASAVTAPSAAAPRQPGMFAQMASTAAGVAVGSAVGHTIGHAMTGGFSGGGSSEAARPDVTYQEPYQPQTMYQQQPMYQQEPVQQNPCTYELKQFLECAQTQSDLKLCEGFSEVLKQCKFSNGLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979E4A6</accession>
    <name>A0A979E4A6_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="4">LOW QUALITY PROTEIN: protein phosphatase 1K, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ppm1kb</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SDA103</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="4">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047008688.1">
        <property type="nucleotide sequence ID" value="XM_047152732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979E4A6"/>
    <dbReference type="GeneID" id="108262886"/>
    <dbReference type="KEGG" id="ipu:108262886"/>
    <dbReference type="CTD" id="503713"/>
    <dbReference type="OrthoDB" id="416093at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF827">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="66"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="RefSeq" id="XP_047008688.1"/>
        </source>
    </evidence>
    <sequence length="288" mass="31902" checksum="CEBE2DEDB6664864" modified="2023-02-22" version="1">MADKLARSLHSPSGTRTSNSRFDPDNSGRPTTWDSFGIWDSRIDEPILLPSSIRYGKLIPKVSLFKVGYISQISQCKENEDRYQVSQMTNDILYFAVFDGHSSAQAADFCHKYMEQYIKEPVAKEDNREVVLTNVFLNVDKALAKHLHFSADGSVVDVCLCLSTAMVALLWDGIELVVASMGDSHAMLCRKGKALKLTVDHTPERKDEKERIRKNGGFVTWNSLGQPHVNGRLAMTPNIGDFDLKNAGVIAEPETKRISDSDVLAKIECACGFGGMVLQATWVAPATF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979EGF0</accession>
    <name>A0A979EGF0_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein LOC108261589</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC108261589</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volume="7" first="11757" last="11757">
            <title>The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts.</title>
            <authorList>
                <person name="Liu Z."/>
                <person name="Liu S."/>
                <person name="Yao J."/>
                <person name="Bao L."/>
                <person name="Zhang J."/>
                <person name="Li Y."/>
                <person name="Jiang C."/>
                <person name="Sun L."/>
                <person name="Wang R."/>
                <person name="Zhang Y."/>
                <person name="Zhou T."/>
                <person name="Zeng Q."/>
                <person name="Fu Q."/>
                <person name="Gao S."/>
                <person name="Li N."/>
                <person name="Koren S."/>
                <person name="Jiang Y."/>
                <person name="Zimin A."/>
                <person name="Xu P."/>
                <person name="Phillippy A.M."/>
                <person name="Geng X."/>
                <person name="Song L."/>
                <person name="Sun F."/>
                <person name="Li C."/>
                <person name="Wang X."/>
                <person name="Chen A."/>
                <person name="Jin Y."/>
                <person name="Yuan Z."/>
                <person name="Yang Y."/>
                <person name="Tan S."/>
                <person name="Peatman E."/>
                <person name="Lu J."/>
                <person name="Qin Z."/>
                <person name="Dunham R."/>
                <person name="Li Z."/>
                <person name="Sonstegard T."/>
                <person name="Feng J."/>
                <person name="Danzmann R.G."/>
                <person name="Schroeder S."/>
                <person name="Scheffler B."/>
                <person name="Duke M.V."/>
                <person name="Ballard L."/>
                <person name="Kucuktas H."/>
                <person name="Kaltenboeck L."/>
                <person name="Liu H."/>
                <person name="Armbruster J."/>
                <person name="Xie Y."/>
                <person name="Kirby M.L."/>
                <person name="Tian Y."/>
                <person name="Flanagan M.E."/>
                <person name="Mu W."/>
                <person name="Waldbieser G.C."/>
            </authorList>
            <dbReference type="PubMed" id="27249958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SDA103</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="RefSeq"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
        <source>
            <tissue evidence="3">Blood</tissue>
        </source>
    </reference>
    <dbReference type="RefSeq" id="XP_047009814.1">
        <property type="nucleotide sequence ID" value="XM_047153858.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A979EGF0"/>
    <dbReference type="GeneID" id="108261589"/>
    <dbReference type="KEGG" id="ipu:108261589"/>
    <dbReference type="OrthoDB" id="8964729at2759"/>
    <dbReference type="Proteomes" id="UP000221080">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000221080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="RefSeq" id="XP_047009814.1"/>
        </source>
    </evidence>
    <sequence length="314" mass="35211" checksum="A0D7886168E1C5D3" modified="2023-02-22" version="1">MIPSSLFLFQCSLQSSTGFHSQIKLLEYISCSIENSFKSSTGYQRSAQNCPKLWNSLPFQDIKKQKREQAMCIYFLGHFFLSSGYESTLQNRLIIFLTTGFCAGFLALLLALGVLYHHHKSRGRISFIAIFLLLSDLLELILSPVLLTYIFNGNADWIHTVITLLFAARFCGHVLHQVVALESIVTVKFPSVAVIFSNSCSITFCIFVWLLAIGCQFIQNAIIYAISMCLFVLPVTTCAACIVTFKAPLDTTQATARKPGGLVLSVAMFTLIILYVPFFLDCLLITPFMLNCLVSMRLISEPLLCIFICRENRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-02-22" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A979ELH8</accession>
    <name>A0A979ELH8_ICTPU</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Interferon-induced protein 44-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC124627504</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ictalurus punctatus</name>
        <name type="common">Channel catfish</name>
        <name type="synonym">Silurus punctatus</name>
        <dbReference type="NCBI Taxonomy" id="7998"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Ostariophysi</taxon>
            <taxon>Siluriformes</taxon>
            <taxon>Ictaluridae</taxon>
            <taxon>Ictalurus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2016" name="Nat. Commun." volum